From ree3 <@t> leicester.ac.uk Tue Apr 1 03:01:06 2008 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Tue Apr 1 03:01:13 2008 Subject: [Histonet] Hematoxylin supplies preserved Message-ID: <7722595275A4DD4FA225B92CDBF174A1038A6463@EXC-MBX3.cfs.le.ac.uk> According to a recent paper in the Archives of Lignacial Reports, a massive petrified forest of Haematoxylum campechianum has been uncovered by melting permafrost in Siberia, local experts are currently testing the logwood to see if it is indeed usable in the production of hematoxylin, so panic over folks!!. Cheers L.B. Jack. The Sherwood Institute. Nottingham England From jqb7 <@t> cdc.gov Tue Apr 1 04:46:34 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Tue Apr 1 04:47:01 2008 Subject: [Histonet] Blackballed In-Reply-To: References: <544090.42586.qm@web38202.mail.mud.yahoo.com> Message-ID: <34BB307EFC9A65429BBB49E330675F720732EE6A@LTA3VS003.ees.hhs.gov> Not all of us have unions....... Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Monday, March 31, 2008 5:48 PM To: Steven Coakley; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Blackballed That's why we have unions. Firstly to ensure that we are all treated as humans!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven Coakley Sent: Tuesday, 1 April 2008 5:50 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Blackballed Well, I suppose I must accept that a certain Lab director has long ago blackballed me from working in Histology, at least, in my area. Guess that's what one gets from rebelling against being so stressed out from excessive periods of short staffing and temps. Working long hours, weekends, on call then being written up for taking advantage of the overtime. Oh well, tis life. I wish this individual great success in weeding out the bad Apple's as myself and others that just can't take the pressure. I hope she never succumbs to such an ordeal in her job. Signing off. You all stay strong and hang in there. No since continuing this membership. --------------------------------- Like movies? Here's a limited-time offer: Blockbuster Total Access for one month at no cost. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Tue Apr 1 07:39:13 2008 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Apr 1 07:39:17 2008 Subject: [Histonet] RELIA Special Job Alert for Managers, Supervisors and Lead Techs 04/01/08 Message-ID: Hello Histonetters, I have several exciting opportunities for experienced Managers, Supervisors and Lead Technicians in hospital and private lab environments in several locations nationwide. The compensation packages are excellent, the work is challenging and the sky is the limit. The positions are of course full time and permanent. Here are my leadership positions: Histology Manager ? Dallas, TX Immunohistochemistry Manager ? Dallas, TX Histology Supervisor ? Houston, TX Pathology Manager ? Portland, OR Histology Manager ? Portland, OR Assistant Supervisor/Lead Tech ?Portland, OR Histology Supervisor ? Los Angeles, CA Lead Histology Tech ? Seattle, WA If you would like more information or know of someone else who might be interested, please contact me at relia1@earthlink.net or 866-607-3542. I am available to discuss the opportunity at your convenience including after hours. Thanks-Pam Thank You! Pam M. Barker President Relia 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 e-mail: relia1@earthlink.net From king.laurie <@t> marshfieldclinic.org Tue Apr 1 07:41:50 2008 From: king.laurie <@t> marshfieldclinic.org (King, Laurie) Date: Tue Apr 1 07:41:56 2008 Subject: [Histonet] Hematoxylin supplies preserved Message-ID: <200804011241.m31CfpCs027091@spamfilt.mfldclin.edu> April Fools???? ------Original Message------ From: "Edwards, R.E." Date: Tue Apr 01, 2008 -- 03:02:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hematoxylin supplies preserved According to a recent paper in the Archives of Lignacial Reports, a massive petrified forest of Haematoxylum campechianum has been uncovered by melting permafrost in Siberia, local experts are currently testing the logwood to see if it is indeed usable in the production of hematoxylin, so panic over folks!!. Cheers L.B. Jack. The Sherwood Institute. Nottingham England _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From oshel1pe <@t> cmich.edu Tue Apr 1 07:50:03 2008 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Tue Apr 1 07:50:16 2008 Subject: [Histonet] Hematoxylin supplies preserved In-Reply-To: <7722595275A4DD4FA225B92CDBF174A1038A6463@EXC-MBX3.cfs.le.ac.uk> References: <7722595275A4DD4FA225B92CDBF174A1038A6463@EXC-MBX3.cfs.le.ac.uk> Message-ID: Yes, an exciting find. Unfortunately, the powder produced is so "pre-aged" it only stains tissues in old, aged Canada balsam. So to date, they've only been able to stain tissues from lizards in amber. Great nuclei, though. Phil >According to a recent paper in the Archives of Lignacial >Reports, a massive petrified forest of Haematoxylum campechianum >has been uncovered by melting permafrost in Siberia, local >experts are currently testing the logwood to see if it is >indeed usable in the production of hematoxylin, so panic over >folks!!. > > Cheers > L.B. Jack. > >The Sherwood Institute. > >Nottingham > England > -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576 From relia1 <@t> earthlink.net Tue Apr 1 09:05:09 2008 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Apr 1 09:05:16 2008 Subject: [Histonet] RELIA Histology Careers Bulletin 04-01-08 Happy April Fools - No Fooling This one is not just for managers! Message-ID: Hi Histonetters!! Happy April Fools Day ? No fooling I have some great opportunities for you to take a look at or pass on to a friend. I have management opportunities and bench positions with excellent salaries, benefits and a great chance for advancement. Remember as always that my services are free of charge to you and completely confidential. We can also customize a job search especially for you. All of the opportunities I represent are permanent positions with premier companies nationwide. They offer excellent salaries benefits and sign-on bonuses/relocation assistance. FYI here is a list of my current openings: Histology/Pathology Management Opportunities Pathology Manager ? Portland, OR Histology Manager ? Portland, OR Assistant Supervisor/Lead Tech ?Portland, OR Histology Supervisor ? Los Angeles, CA Lead Histology Tech ? Seattle, WA Histology Manager ? Dallas, TX Immunohistochemistry Manager ? Dallas, TX Histology Supervisor ? Houston, TX My Histotechnician/Histotechnologist positions are located in: Philadelphia area, PA Pittsburgh, PA Arlington, VA Harrisonburg, VA Austin, TX Corpus, Christi, TX Los Angeles, CA If you or any of your friends would like to chat about customizing a job search or would like more information about any of the positions listed please contact me. I would be more than happy to assist you. You can reach me at 866-607-3542 or relia1@earthlink.net I am available to chat after hours by appointment as well. You don?t need to talk to another histotech to find the right opportunity you need a competent experienced committed recruiter to help you get the job you want. I offer over 25 years of recruiting and job counseling expertise. Remember it never hurts to look Hope to hear from you soon . Thanks-Pam Thank You! Pam M. Barker President Relia 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll Free: (866)607-3542 e-mail: relia1@earthlink.net http://home.earthlink.net/~relia1 www.myspace.com/pamatrelia From ian.montgomery <@t> bio.gla.ac.uk Tue Apr 1 09:13:19 2008 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Tue Apr 1 09:13:30 2008 Subject: FW: [Histonet] Blackballed Message-ID: <005701c89402$898bb8e0$6424d182@IBLS.GLA.AC.UK> Surely in the World's greatest, allegedly, democracy you have employment laws that will protect workers from this type of behaviour? Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: 01 April 2008 10:47 To: Tony Henwood; Steven Coakley; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Blackballed Not all of us have unions....... Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Monday, March 31, 2008 5:48 PM To: Steven Coakley; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Blackballed That's why we have unions. Firstly to ensure that we are all treated as humans!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven Coakley Sent: Tuesday, 1 April 2008 5:50 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Blackballed Well, I suppose I must accept that a certain Lab director has long ago blackballed me from working in Histology, at least, in my area. Guess that's what one gets from rebelling against being so stressed out from excessive periods of short staffing and temps. Working long hours, weekends, on call then being written up for taking advantage of the overtime. Oh well, tis life. I wish this individual great success in weeding out the bad Apple's as myself and others that just can't take the pressure. I hope she never succumbs to such an ordeal in her job. Signing off. You all stay strong and hang in there. No since continuing this membership. --------------------------------- Like movies? Here's a limited-time offer: Blockbuster Total Access for one month at no cost. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victoria.spoon <@t> bassett.org Tue Apr 1 09:11:18 2008 From: victoria.spoon <@t> bassett.org (Spoon, Victoria) Date: Tue Apr 1 09:14:06 2008 Subject: [Histonet] Per diem position available in Cooperstown NY Message-ID: <415700FC732DE14491A3E39367834F7701245A34@ex3.bassett.org> Bassett Hospital in Cooperstown NY, has a per diem histotechnician position available. Responsibilities include many aspects of histology (embedding, cutting, staining of human tissue) covering for coworkers time off. Applicants must possess an Associates degree and meet NYS licensure qualifications for a histotechnician. Bassett Hospital is a rural picturesque lakeside village, located in Upstate New York. Cooperstown offers year round cultural and recreational opportunities. Bassett employs 2000 employees in it's teaching and research system. Interested applicants can call Human Resources at (607)547-3120. or click on www.bassett.org NOTICE OF CONFIDENTIALITY This electronic message, including attachments, is for the sole use of the named recipient and may contain confidential or privileged information protected by New York State, and Federal regulations. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. If you are not the intended recipient or have received this communication in error please contact the sender or email.security@bassett.org and destroy all copies of the original message. Thank you. From Bryan.Watson <@t> parkview.com Tue Apr 1 10:32:00 2008 From: Bryan.Watson <@t> parkview.com (Bryan Watson) Date: Tue Apr 1 10:32:22 2008 Subject: FW: [Histonet] Blackballed In-Reply-To: <005701c89402$898bb8e0$6424d182@IBLS.GLA.AC.UK> References: <005701c89402$898bb8e0$6424d182@IBLS.GLA.AC.UK> Message-ID: <47F21D30.ACD8.0085.0@parkview.com> I've not been able to ever witness this alleged democracy myself. I've lived here my whole life. . . >>> "Ian Montgomery" 4/1/2008 10:13 >>> Surely in the World's greatest, allegedly, democracy you have employment laws that will protect workers from this type of behaviour? Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: 01 April 2008 10:47 To: Tony Henwood; Steven Coakley; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Blackballed Not all of us have unions....... Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Monday, March 31, 2008 5:48 PM To: Steven Coakley; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Blackballed That's why we have unions. Firstly to ensure that we are all treated as humans!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven Coakley Sent: Tuesday, 1 April 2008 5:50 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Blackballed Well, I suppose I must accept that a certain Lab director has long ago blackballed me from working in Histology, at least, in my area. Guess that's what one gets from rebelling against being so stressed out from excessive periods of short staffing and temps. Working long hours, weekends, on call then being written up for taking advantage of the overtime. Oh well, tis life. I wish this individual great success in weeding out the bad Apple's as myself and others that just can't take the pressure. I hope she never succumbs to such an ordeal in her job. Signing off. You all stay strong and hang in there. No since continuing this membership. --------------------------------- Like movies? Here's a limited-time offer: Blockbuster Total Access for one month at no cost. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Tue Apr 1 10:57:18 2008 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Tue Apr 1 10:57:31 2008 Subject: [Histonet] Bottomless cup of coffee Message-ID: <5C0BED61F529364E86309CADEA63FEF20163F3B3@TRFT-EX01.xRothGen.nhs.uk> I first came across this concept in Ottawa in 1962 - a cup of coffee that was replenished at no extra charge. In Oman in 1989, I came across the Indian vegetarian thali that was constantly replenished. (Pearl restaurant in Ruwi - highly recommended). Now I have come across the bottomless pot of haematoxylin, which has been in use in this lab since I came 8 years ago. Apparently it is just topped up now and then, and rarely discarded for fresh haematoxylin. Is this normal/standard/acceptable. I am shocked to the core! Terry From Charles.Embrey <@t> carle.com Tue Apr 1 10:59:33 2008 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Tue Apr 1 10:59:41 2008 Subject: [Histonet] Blackballed In-Reply-To: <005701c89402$898bb8e0$6424d182@IBLS.GLA.AC.UK> References: <005701c89402$898bb8e0$6424d182@IBLS.GLA.AC.UK> Message-ID: <44780C571F28624DBB446DE55C4D733A1FE5A1@EXCHANGEBE1.carle.com> Ian, I find your comment rather offensive and uncalled for. I hope you didn't mean it way it comes across. Charles Embrey, PA(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian Montgomery Sent: Tuesday, April 01, 2008 9:13 AM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] Blackballed Surely in the World's greatest, allegedly, democracy you have employment laws that will protect workers from this type of behaviour? Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: 01 April 2008 10:47 To: Tony Henwood; Steven Coakley; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Blackballed Not all of us have unions....... Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Monday, March 31, 2008 5:48 PM To: Steven Coakley; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Blackballed That's why we have unions. Firstly to ensure that we are all treated as humans!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven Coakley Sent: Tuesday, 1 April 2008 5:50 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Blackballed Well, I suppose I must accept that a certain Lab director has long ago blackballed me from working in Histology, at least, in my area. Guess that's what one gets from rebelling against being so stressed out from excessive periods of short staffing and temps. Working long hours, weekends, on call then being written up for taking advantage of the overtime. Oh well, tis life. I wish this individual great success in weeding out the bad Apple's as myself and others that just can't take the pressure. I hope she never succumbs to such an ordeal in her job. Signing off. You all stay strong and hang in there. No since continuing this membership. --------------------------------- Like movies? Here's a limited-time offer: Blockbuster Total Access for one month at no cost. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmiller <@t> physlab.com Tue Apr 1 11:01:22 2008 From: cmiller <@t> physlab.com (Cheri Miller) Date: Tue Apr 1 11:02:02 2008 Subject: FW: [Histonet] Blackballed In-Reply-To: <47F21D30.ACD8.0085.0@parkview.com> References: <005701c89402$898bb8e0$6424d182@IBLS.GLA.AC.UK> <47F21D30.ACD8.0085.0@parkview.com> Message-ID: <000601c89411$a1662540$3d02a8c0@plab.local> Go hang out in a Non-Democratic society for a few months then come back and tell us how you have never experienced it here in your homeland. You are clearly a product of a democratic nation; you take your liberties for granted so that you don't even know when you have them. If we don't like it we have the right to protest, to take action and change it! That's democracy. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bryan Watson Sent: Tuesday, April 01, 2008 10:32 AM To: ian.montgomery@bio.gla.ac.uk; histonet@lists.utsouthwestern.edu Subject: Re: FW: [Histonet] Blackballed I've not been able to ever witness this alleged democracy myself. I've lived here my whole life. . . >>> "Ian Montgomery" 4/1/2008 10:13 >>> Surely in the World's greatest, allegedly, democracy you have employment laws that will protect workers from this type of behaviour? Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: 01 April 2008 10:47 To: Tony Henwood; Steven Coakley; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Blackballed Not all of us have unions....... Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Monday, March 31, 2008 5:48 PM To: Steven Coakley; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Blackballed That's why we have unions. Firstly to ensure that we are all treated as humans!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven Coakley Sent: Tuesday, 1 April 2008 5:50 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Blackballed Well, I suppose I must accept that a certain Lab director has long ago blackballed me from working in Histology, at least, in my area. Guess that's what one gets from rebelling against being so stressed out from excessive periods of short staffing and temps. Working long hours, weekends, on call then being written up for taking advantage of the overtime. Oh well, tis life. I wish this individual great success in weeding out the bad Apple's as myself and others that just can't take the pressure. I hope she never succumbs to such an ordeal in her job. Signing off. You all stay strong and hang in there. No since continuing this membership. --------------------------------- Like movies? Here's a limited-time offer: Blockbuster Total Access for one month at no cost. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From CIngles <@t> uwhealth.org Tue Apr 1 11:18:17 2008 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Tue Apr 1 11:18:24 2008 Subject: [Histonet] Democracy References: <005701c89402$898bb8e0$6424d182@IBLS.GLA.AC.UK><47F21D30.ACD8.0085.0@parkview.com> <000601c89411$a1662540$3d02a8c0@plab.local> Message-ID: <08A0A863637F1349BBFD83A96B27A50A120112@uwhis-xchng3.uwhis.hosp.wisc.edu> Can't we just all play nice once in a while, instead of flying off the handle for one comment? OK, 2 or 3 would be a problem, but I am getting a bit tired of the vocal minority, on opposite ends of the spectrum. I think a part of the Democracy thing is that we should respect the idea of respect in freedom of speech. Sure people say all kinds of things, but we don't need to instantly attack them. We need to be respectful of others ideas and actions (as long as they aren't hurting anyone of course) even if we don't agree with them. They have their lives and let them live the way that makes them happy. (gee - pursuit of happiness...) I agree about the democratic liberties being taken for granted. I have visited over 15 countries in my youth and lived in Saudi Arabia for 4 years. I DO NOT take America for granted. I am even ashamed to call myself American on occasion (current administration comes to mind), but I am always grateful to have been born American to have the opportunities and quality of life I have. Opps, sorry I'll get off the soapbox now. Claire ________________________________ Go hang out in a Non-Democratic society for a few months then come back and tell us how you have never experienced it here in your homeland. You are clearly a product of a democratic nation; you take your liberties for granted so that you don't even know when you have them. If we don't like it we have the right to protest, to take action and change it! That's democracy. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 From JWEEMS <@t> sjha.org Tue Apr 1 11:23:54 2008 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Tue Apr 1 11:24:36 2008 Subject: FW: [Histonet] Blackballed In-Reply-To: <47F21D30.ACD8.0085.0@parkview.com> References: <005701c89402$898bb8e0$6424d182@IBLS.GLA.AC.UK> <47F21D30.ACD8.0085.0@parkview.com> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD320518EB76@sjhaexc02.sjha.org> I'm shocked that you would take our liberties for granted. Things aren't perfect anywhere, but in many places you could not have said what you just said without losing your head. So look again....j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bryan Watson Sent: Tuesday, April 01, 2008 11:32 AM To: ian.montgomery@bio.gla.ac.uk; histonet@lists.utsouthwestern.edu Subject: Re: FW: [Histonet] Blackballed I've not been able to ever witness this alleged democracy myself. I've lived here my whole life. . . >>> "Ian Montgomery" 4/1/2008 10:13 >>> Surely in the World's greatest, allegedly, democracy you have employment laws that will protect workers from this type of behaviour? Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: 01 April 2008 10:47 To: Tony Henwood; Steven Coakley; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Blackballed Not all of us have unions....... Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Monday, March 31, 2008 5:48 PM To: Steven Coakley; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Blackballed That's why we have unions. Firstly to ensure that we are all treated as humans!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven Coakley Sent: Tuesday, 1 April 2008 5:50 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Blackballed Well, I suppose I must accept that a certain Lab director has long ago blackballed me from working in Histology, at least, in my area. Guess that's what one gets from rebelling against being so stressed out from excessive periods of short staffing and temps. Working long hours, weekends, on call then being written up for taking advantage of the overtime. Oh well, tis life. I wish this individual great success in weeding out the bad Apple's as myself and others that just can't take the pressure. I hope she never succumbs to such an ordeal in her job. Signing off. You all stay strong and hang in there. No since continuing this membership. --------------------------------- Like movies? Here's a limited-time offer: Blockbuster Total Access for one month at no cost. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From JWEEMS <@t> sjha.org Tue Apr 1 11:29:11 2008 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Tue Apr 1 11:29:40 2008 Subject: [Histonet] Blackballed In-Reply-To: <44780C571F28624DBB446DE55C4D733A1FE5A1@EXCHANGEBE1.carle.com> References: <005701c89402$898bb8e0$6424d182@IBLS.GLA.AC.UK> <44780C571F28624DBB446DE55C4D733A1FE5A1@EXCHANGEBE1.carle.com> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD320518EB78@sjhaexc02.sjha.org> I will shut up after this.... Charles, I think you misunderstood Ian. I understood him to just be making a comment that he thought we would have laws to cover things like that. Ian, we have lawyers that could fix anything if one has enough money. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles.Embrey Sent: Tuesday, April 01, 2008 12:00 PM To: ian.montgomery@bio.gla.ac.uk; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Blackballed Ian, I find your comment rather offensive and uncalled for. I hope you didn't mean it way it comes across. Charles Embrey, PA(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian Montgomery Sent: Tuesday, April 01, 2008 9:13 AM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] Blackballed Surely in the World's greatest, allegedly, democracy you have employment laws that will protect workers from this type of behaviour? Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: 01 April 2008 10:47 To: Tony Henwood; Steven Coakley; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Blackballed Not all of us have unions....... Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Monday, March 31, 2008 5:48 PM To: Steven Coakley; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Blackballed That's why we have unions. Firstly to ensure that we are all treated as humans!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven Coakley Sent: Tuesday, 1 April 2008 5:50 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Blackballed Well, I suppose I must accept that a certain Lab director has long ago blackballed me from working in Histology, at least, in my area. Guess that's what one gets from rebelling against being so stressed out from excessive periods of short staffing and temps. Working long hours, weekends, on call then being written up for taking advantage of the overtime. Oh well, tis life. I wish this individual great success in weeding out the bad Apple's as myself and others that just can't take the pressure. I hope she never succumbs to such an ordeal in her job. Signing off. You all stay strong and hang in there. No since continuing this membership. --------------------------------- Like movies? Here's a limited-time offer: Blockbuster Total Access for one month at no cost. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From jqb7 <@t> cdc.gov Tue Apr 1 11:29:49 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Tue Apr 1 11:31:48 2008 Subject: [Histonet] Democracy In-Reply-To: <08A0A863637F1349BBFD83A96B27A50A120112@uwhis-xchng3.uwhis.hosp.wisc.edu> References: <005701c89402$898bb8e0$6424d182@IBLS.GLA.AC.UK><47F21D30.ACD8.0085.0@parkview.com> <000601c89411$a1662540$3d02a8c0@plab.local> <08A0A863637F1349BBFD83A96B27A50A120112@uwhis-xchng3.uwhis.hosp.wisc.edu> Message-ID: <34BB307EFC9A65429BBB49E330675F720732EE7C@LTA3VS003.ees.hhs.gov> We probably should not judge how we feel about being American based on any Administration. (I had grave issues with the prior Administration!) I have never been ashamed to call myself American even when my President was perjuring himself. I am proud to be an American always. Oops. Now I am off my soap box.............. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Tuesday, April 01, 2008 12:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Democracy Can't we just all play nice once in a while, instead of flying off the handle for one comment? OK, 2 or 3 would be a problem, but I am getting a bit tired of the vocal minority, on opposite ends of the spectrum. I think a part of the Democracy thing is that we should respect the idea of respect in freedom of speech. Sure people say all kinds of things, but we don't need to instantly attack them. We need to be respectful of others ideas and actions (as long as they aren't hurting anyone of course) even if we don't agree with them. They have their lives and let them live the way that makes them happy. (gee - pursuit of happiness...) I agree about the democratic liberties being taken for granted. I have visited over 15 countries in my youth and lived in Saudi Arabia for 4 years. I DO NOT take America for granted. I am even ashamed to call myself American on occasion (current administration comes to mind), but I am always grateful to have been born American to have the opportunities and quality of life I have. Opps, sorry I'll get off the soapbox now. Claire ________________________________ Go hang out in a Non-Democratic society for a few months then come back and tell us how you have never experienced it here in your homeland. You are clearly a product of a democratic nation; you take your liberties for granted so that you don't even know when you have them. If we don't like it we have the right to protest, to take action and change it! That's democracy. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMahoney <@t> alegent.org Tue Apr 1 11:34:08 2008 From: JMahoney <@t> alegent.org (Mahoney,Janice A) Date: Tue Apr 1 11:34:15 2008 Subject: [Histonet] Blackballed In-Reply-To: <44780C571F28624DBB446DE55C4D733A1FE5A1@EXCHANGEBE1.carle.com> References: <005701c89402$898bb8e0$6424d182@IBLS.GLA.AC.UK>, <44780C571F28624DBB446DE55C4D733A1FE5A1@EXCHANGEBE1.carle.com> Message-ID: <346E5878979BA54FB4B0BFD6AD93B9B9B01EE961DD@EXCHMBC1.ad.ah.local> Lets get back to our common vision and mission as Histotechnologists and do the best we can for science and patient care. Please quit with the political rhetoric and spewing of ideological opinions. We (the histonet) are an international, diverse group. That is part of what makes us so great. Life is very short and there's no time for fussing and fighting my friends! Jan Omaha, NE Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From eearle <@t> ccpathology.com Tue Apr 1 11:49:22 2008 From: eearle <@t> ccpathology.com (Elizabeth Earle) Date: Tue Apr 1 11:49:32 2008 Subject: [Histonet] LEAN Histology labs in California Message-ID: Have any histo labs in California implemented LEAN management/workflow? I'd be very interested in hearing from you and visiting your lab if possible. Thanks Elizabeth From doug <@t> ppspath.com Tue Apr 1 12:46:40 2008 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue Apr 1 11:50:01 2008 Subject: SPAM-LOW: [Histonet] Democracy In-Reply-To: <08A0A863637F1349BBFD83A96B27A50A120112@uwhis-xchng3.uwhis.hosp.wisc.edu> Message-ID: If only we could make Hematoxylin out of soap boxes! :) Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Tuesday, April 01, 2008 11:18 AM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] Democracy Can't we just all play nice once in a while, instead of flying off the handle for one comment? OK, 2 or 3 would be a problem, but I am getting a bit tired of the vocal minority, on opposite ends of the spectrum. I think a part of the Democracy thing is that we should respect the idea of respect in freedom of speech. Sure people say all kinds of things, but we don't need to instantly attack them. We need to be respectful of others ideas and actions (as long as they aren't hurting anyone of course) even if we don't agree with them. They have their lives and let them live the way that makes them happy. (gee - pursuit of happiness...) I agree about the democratic liberties being taken for granted. I have visited over 15 countries in my youth and lived in Saudi Arabia for 4 years. I DO NOT take America for granted. I am even ashamed to call myself American on occasion (current administration comes to mind), but I am always grateful to have been born American to have the opportunities and quality of life I have. Opps, sorry I'll get off the soapbox now. Claire ________________________________ Go hang out in a Non-Democratic society for a few months then come back and tell us how you have never experienced it here in your homeland. You are clearly a product of a democratic nation; you take your liberties for granted so that you don't even know when you have them. If we don't like it we have the right to protest, to take action and change it! That's democracy. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Tue Apr 1 11:56:18 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Tue Apr 1 11:56:26 2008 Subject: [Histonet] Democracy In-Reply-To: <34BB307EFC9A65429BBB49E330675F720732EE7C@LTA3VS003.ees.hhs.gov> References: <005701c89402$898bb8e0$6424d182@IBLS.GLA.AC.UK> <47F21D30.ACD8.0085.0@parkview.com> <000601c89411$a1662540$3d02a8c0@plab.local> <08A0A863637F1349BBFD83A96B27A50A120112@uwhis-xchng3.uwhis.hosp.wisc.edu> <34BB307EFC9A65429BBB49E330675F720732EE7C@LTA3VS003.ees.hhs.gov> Message-ID: <5b6eb13e0804010956o3693fc9cp933fbf05d6648062@mail.gmail.com> Our country got bought out in huge corporate deals. Nobody else noticed? On Tue, Apr 1, 2008 at 9:29 AM, Bartlett, Jeanine (CDC/CCID/NCZVED) < jqb7@cdc.gov> wrote: > We probably should not judge how we feel about being American based on > any Administration. (I had grave issues with the prior Administration!) > I have never been ashamed to call myself American even when my President > was perjuring himself. > > I am proud to be an American always. > > Oops. Now I am off my soap box.............. > > > Jeanine Bartlett > Infectious Diseases Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles > Claire > Sent: Tuesday, April 01, 2008 12:18 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Democracy > > Can't we just all play nice once in a while, instead of flying off the > handle for one comment? OK, 2 or 3 would be a problem, but I am getting > a bit tired of the vocal minority, on opposite ends of the spectrum. I > think a part of the Democracy thing is that we should respect the idea > of respect in freedom of speech. Sure people say all kinds of things, > but we don't need to instantly attack them. We need to be respectful of > others ideas and actions (as long as they aren't hurting anyone of > course) even if we don't agree with them. They have their lives and let > them live the way that makes them happy. (gee - pursuit of happiness...) > I agree about the democratic liberties being taken for granted. I have > visited over 15 countries in my youth and lived in Saudi Arabia for 4 > years. I DO NOT take America for granted. I am even ashamed to call > myself American on occasion (current administration comes to mind), but > I am always grateful to have been born American to have the > opportunities and quality of life I have. Opps, sorry I'll get off the > soapbox now. > > Claire > > ________________________________ > > > > > Go hang out in a Non-Democratic society for a few months then come back > and tell us how you have never experienced it here in your homeland. You > are clearly a product of a democratic nation; you take your liberties > for granted so that you don't even know when you have them. If we don't > like it we have the right to protest, to take action and change it! > That's democracy. > Cheryl Miller HT (ASCP) > Histology Supervisor > Physicians Laboratory,P.C. > Omaha, Ne. > 402 738 5052 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gu.lang <@t> gmx.at Tue Apr 1 11:57:07 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Apr 1 11:57:17 2008 Subject: AW: [Histonet] Bottomless cup of coffee In-Reply-To: <5C0BED61F529364E86309CADEA63FEF20163F3B3@TRFT-EX01.xRothGen.nhs.uk> Message-ID: <000001c89419$6c423270$eeeea8c0@dielangs.at> If it works, ... it's acceptable. Or have you noticed any problems in staining, that can be caused by this? And perhaps there are standardizised times and amounts for filling up ;). I also remember something similiar in our lab before automated staining. And it was a kind of religion, that the haemalaun (Mayers) even would turn better with time in the pot. We always had beautiful HEs. (And now we have also.) Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Marshall Terry Dr,Consultant Histopathologist Gesendet: Dienstag, 01. April 2008 17:57 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Bottomless cup of coffee I first came across this concept in Ottawa in 1962 - a cup of coffee that was replenished at no extra charge. In Oman in 1989, I came across the Indian vegetarian thali that was constantly replenished. (Pearl restaurant in Ruwi - highly recommended). Now I have come across the bottomless pot of haematoxylin, which has been in use in this lab since I came 8 years ago. Apparently it is just topped up now and then, and rarely discarded for fresh haematoxylin. Is this normal/standard/acceptable. I am shocked to the core! Terry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Tue Apr 1 12:12:32 2008 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Tue Apr 1 12:13:02 2008 Subject: [Histonet] Bottomless cup of coffee In-Reply-To: <000001c89419$6c423270$eeeea8c0@dielangs.at> References: <5C0BED61F529364E86309CADEA63FEF20163F3B3@TRFT-EX01.xRothGen.nhs.uk> <000001c89419$6c423270$eeeea8c0@dielangs.at> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD320518EB7C@sjhaexc02.sjha.org> What kind of hematoxylin? I thought this would be impossible since we no longer use mercury! Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Tuesday, April 01, 2008 12:57 PM To: 'Marshall Terry Dr,Consultant Histopathologist' Cc: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] Bottomless cup of coffee If it works, ... it's acceptable. Or have you noticed any problems in staining, that can be caused by this? And perhaps there are standardizised times and amounts for filling up ;). I also remember something similiar in our lab before automated staining. And it was a kind of religion, that the haemalaun (Mayers) even would turn better with time in the pot. We always had beautiful HEs. (And now we have also.) Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Marshall Terry Dr,Consultant Histopathologist Gesendet: Dienstag, 01. April 2008 17:57 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Bottomless cup of coffee I first came across this concept in Ottawa in 1962 - a cup of coffee that was replenished at no extra charge. In Oman in 1989, I came across the Indian vegetarian thali that was constantly replenished. (Pearl restaurant in Ruwi - highly recommended). Now I have come across the bottomless pot of haematoxylin, which has been in use in this lab since I came 8 years ago. Apparently it is just topped up now and then, and rarely discarded for fresh haematoxylin. Is this normal/standard/acceptable. I am shocked to the core! Terry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From contact <@t> excaliburpathology.com Tue Apr 1 12:56:37 2008 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Tue Apr 1 12:56:41 2008 Subject: [Histonet] Blackballed Message-ID: <871529.45017.qm@web50103.mail.re2.yahoo.com> Firstly, the USA is a representative republic, not a democracy. No matter the administration, it is still one of the best places to live. And IMHO, unions are what killed the auto industry and allows poor teachers to continue teaching. Now back to Steve's original post. If you truly belive this is happening to you, be up front about the situation with any future employer or don't list that reference. Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 631 N. Broadway Ave. Moore, OK 73160 405-570-6679 cell 405-759-3953 lab contact@excaliburpathology.com www.excaliburpathology.com From SohrabB1 <@t> ah.org Tue Apr 1 13:54:57 2008 From: SohrabB1 <@t> ah.org (Behnaz Sohrab) Date: Tue Apr 1 13:55:44 2008 Subject: [Histonet] Alzheimer stain Message-ID: <47F2228F.4347.0054.0@ah.org> Question regarding Sodium Carbonate Solution in Bielschowsky method for Alzheimer. Can I substitute Carbonate? Or any one has a better method for this stain? ( I do not have Sodium Carbonate, and no time to order it). Thanks,Behnaz From Barry.R.Rittman <@t> uth.tmc.edu Tue Apr 1 13:55:46 2008 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Tue Apr 1 13:55:52 2008 Subject: [Histonet] Democracy In-Reply-To: <08A0A863637F1349BBFD83A96B27A50A120112@uwhis-xchng3.uwhis.hosp.wisc.edu> References: <005701c89402$898bb8e0$6424d182@IBLS.GLA.AC.UK> <47F21D30.ACD8.0085.0@parkview.com> <000601c89411$a1662540$3d02a8c0@plab.local> <08A0A863637F1349BBFD83A96B27A50A120112@uwhis-xchng3.uwhis.hosp.wisc.edu> Message-ID: Please, Histonet is not supposed to be a political or religious sounding board. If you wish to criticize this or any other country or any religion then please take this elsewhere. Let us stick to histology. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Tuesday, April 01, 2008 11:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Democracy Can't we just all play nice once in a while, instead of flying off the handle for one comment? OK, 2 or 3 would be a problem, but I am getting a bit tired of the vocal minority, on opposite ends of the spectrum. I think a part of the Democracy thing is that we should respect the idea of respect in freedom of speech. Sure people say all kinds of things, but we don't need to instantly attack them. We need to be respectful of others ideas and actions (as long as they aren't hurting anyone of course) even if we don't agree with them. They have their lives and let them live the way that makes them happy. (gee - pursuit of happiness...) I agree about the democratic liberties being taken for granted. I have visited over 15 countries in my youth and lived in Saudi Arabia for 4 years. I DO NOT take America for granted. I am even ashamed to call myself American on occasion (current administration comes to mind), but I am always grateful to have been born American to have the opportunities and quality of life I have. Opps, sorry I'll get off the soapbox now. Claire ________________________________ Go hang out in a Non-Democratic society for a few months then come back and tell us how you have never experienced it here in your homeland. You are clearly a product of a democratic nation; you take your liberties for granted so that you don't even know when you have them. If we don't like it we have the right to protest, to take action and change it! That's democracy. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Tue Apr 1 14:24:42 2008 From: shive003 <@t> umn.edu (Jan Shivers) Date: Tue Apr 1 14:24:49 2008 Subject: [Histonet] Democracy References: <005701c89402$898bb8e0$6424d182@IBLS.GLA.AC.UK><47F21D30.ACD8.0085.0@parkview.com><000601c89411$a1662540$3d02a8c0@plab.local><08A0A863637F1349BBFD83A96B27A50A120112@uwhis-xchng3.uwhis.hosp.wisc.edu> Message-ID: <00a401c8942e$0966fd10$b0065486@auxs.umn.edu> I second that... and/or reply to the sender privately. Thank you. Jan Shivers ----- Original Message ----- From: "Rittman, Barry R" To: Sent: Tuesday, April 01, 2008 1:55 PM Subject: RE: [Histonet] Democracy Please, Histonet is not supposed to be a political or religious sounding board. If you wish to criticize this or any other country or any religion then please take this elsewhere. Let us stick to histology. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Tuesday, April 01, 2008 11:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Democracy Can't we just all play nice once in a while, instead of flying off the handle for one comment? OK, 2 or 3 would be a problem, but I am getting a bit tired of the vocal minority, on opposite ends of the spectrum. I think a part of the Democracy thing is that we should respect the idea of respect in freedom of speech. Sure people say all kinds of things, but we don't need to instantly attack them. We need to be respectful of others ideas and actions (as long as they aren't hurting anyone of course) even if we don't agree with them. They have their lives and let them live the way that makes them happy. (gee - pursuit of happiness...) I agree about the democratic liberties being taken for granted. I have visited over 15 countries in my youth and lived in Saudi Arabia for 4 years. I DO NOT take America for granted. I am even ashamed to call myself American on occasion (current administration comes to mind), but I am always grateful to have been born American to have the opportunities and quality of life I have. Opps, sorry I'll get off the soapbox now. Claire ________________________________ Go hang out in a Non-Democratic society for a few months then come back and tell us how you have never experienced it here in your homeland. You are clearly a product of a democratic nation; you take your liberties for granted so that you don't even know when you have them. If we don't like it we have the right to protest, to take action and change it! That's democracy. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Tue Apr 1 14:37:54 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Tue Apr 1 14:37:59 2008 Subject: [Histonet] Democracy In-Reply-To: References: <005701c89402$898bb8e0$6424d182@IBLS.GLA.AC.UK> <47F21D30.ACD8.0085.0@parkview.com> <000601c89411$a1662540$3d02a8c0@plab.local> <08A0A863637F1349BBFD83A96B27A50A120112@uwhis-xchng3.uwhis.hosp.wisc.edu> Message-ID: <5b6eb13e0804011237h60dbce82g14a15ec1ed7d0817@mail.gmail.com> Barry- Wasn't it you, who just last week, was advocating for Canada Balsam?? Pretty hypocritical if you ask me. On Tue, Apr 1, 2008 at 11:55 AM, Rittman, Barry R < Barry.R.Rittman@uth.tmc.edu> wrote: > Please, > Histonet is not supposed to be a political or religious sounding board. > If you wish to criticize this or any other country or any religion then > please take this elsewhere. > Let us stick to histology. > Barry > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles > Claire > Sent: Tuesday, April 01, 2008 11:18 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Democracy > > Can't we just all play nice once in a while, instead of flying off the > handle for one comment? OK, 2 or 3 would be a problem, but I am getting > a bit tired of the vocal minority, on opposite ends of the spectrum. I > think a part of the Democracy thing is that we should respect the idea > of respect in freedom of speech. Sure people say all kinds of things, > but we don't need to instantly attack them. We need to be respectful of > others ideas and actions (as long as they aren't hurting anyone of > course) even if we don't agree with them. They have their lives and let > them live the way that makes them happy. (gee - pursuit of happiness...) > I agree about the democratic liberties being taken for granted. I have > visited over 15 countries in my youth and lived in Saudi Arabia for 4 > years. I DO NOT take America for granted. I am even ashamed to call > myself American on occasion (current administration comes to mind), but > I am always grateful to have been born American to have the > opportunities and quality of life I have. Opps, sorry I'll get off the > soapbox now. > > Claire > > ________________________________ > > > > > Go hang out in a Non-Democratic society for a few months then come back > and > tell us how you have never experienced it here in your homeland. You are > clearly a product of a democratic nation; you take your liberties for > granted so that you don't even know when you have them. If we don't like > it > we have the right to protest, to take action and change it! That's > democracy. > Cheryl Miller HT (ASCP) > Histology Supervisor > Physicians Laboratory,P.C. > Omaha, Ne. > 402 738 5052 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ROrr <@t> enh.org Tue Apr 1 14:47:20 2008 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Tue Apr 1 14:47:32 2008 Subject: [Histonet] RE:LEAN Histology labs in California Message-ID: HI Elizabeth, Even though I'm not close by, I would be glad to share my experiences with the LEAN process...Our lab has improved drastically since we brought in these principles. Even though we did buy instrumentation, sometimes all it took was re arranging some of the older equipment in the lab to an another area that resulted in some great improvements! ....Now if I could only get some of this LEAN stuff onto my hips! Have a great day. Becky Have any histo labs in California implemented LEAN management/workflow? I'd be very interested in hearing from you and visiting your lab if possible. Thanks Elizabeth Becky Orr CLA,HT(ASCP)QIHC Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 From gmartin <@t> marshallmedical.org Tue Apr 1 14:47:37 2008 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Tue Apr 1 14:47:51 2008 Subject: [Histonet] Labeling cassettes Message-ID: <6ED9D4252F278841A0593D3D788AF24C02159A81@mailsvr.MARSHMED.local> Our Pathologist presently label the cassettes at the time of grossing. We have decided it would be better for the someone else to do that task. We have a very small grossing area, and we will be labeling by hand. I am looking to the group for ideas concerning; 1) When are cassettes labeled ... when the specimens are stocked or at accessioning ... 2) How are the cassettes stored before the days grossing take place. Remembering we have a small area ... the table will need to be clear for frozen sections, etc. 3) Is there a resource for typical number of cassettes for particular specimens. The pathologists are having difficulty agreeing on that issue. Thanks as always Gary From juditw <@t> u.washington.edu Tue Apr 1 14:52:30 2008 From: juditw <@t> u.washington.edu (Judith L. Williams) Date: Tue Apr 1 14:52:37 2008 Subject: [Histonet] Re: DPX Canada balsam In-Reply-To: Message-ID: Hi to Robert and all - I agree about the sweet smell of canada balsam! I was trained in the 70's and one old taxonomist I worked with showed me how to mount tiny inverts with canada balsam and he gave me a few 'sticks' of it. I keep them to show students and trainees just what it is, how it looks, how hard it is, yellow and why there are slides from the 1800 in the Smithsonian of inverts mounted in it that look wonderful.! Ah memories - and yes, I do remember the hematoxylin shortage - it was real - at least it did become nearly impossible to get the power! happy tuesday to histoland Judy at UW in Washington On Mon, 31 Mar 2008, Robert Richmond wrote: > Sheesh, I'm nearly 70 and I barely remember Canada balsam! It's a > wonderfully sweet-smelling resin. Lillie described it as almost pure > abietic acid. It would destroy most stains within a few years - > neutralization of the balsam with potassium carbonate was said to > prevent this, though I don't see how. > > Canada balsam took at least a month to set solidly enough that you > could file the slides. This "curing" could be speeded up by putting > the slides in flat trays in a 37 degree C incubator for a week. At > Johns Hopkins an ancient walk-in bacteriology incubator was still in > use - I loved to go in there because it smelled so nice. The synthetic > mounting medium in use around 1965 also benefited from a few days' > curing, so the incubator remained in use. > > Around 1970 I took the coverslip off a 1932 Levaditi silver stain of > syphilitic infant liver, so I could replace it with a coverslip thin > enough for me to photograph the little black spirochetes under an oil > immersion objective. Took a week in warm xylene to get the thing to > fall off. > > Canada balsam is still around - look at a can of orange soda and > you'll see it contains "glycerol ester of wood rosin". Yummers! > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Judith Williams, PhD, HT(ASCP) Research Scientist Department of Comparative Medicine University of Washington Seattle, WA 98195 From JMahoney <@t> alegent.org Tue Apr 1 15:02:40 2008 From: JMahoney <@t> alegent.org (Mahoney,Janice A) Date: Tue Apr 1 15:06:44 2008 Subject: [Histonet] RE: Labeling cassettes In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C02159A81@mailsvr.MARSHMED.local> References: <6ED9D4252F278841A0593D3D788AF24C02159A81@mailsvr.MARSHMED.local> Message-ID: <346E5878979BA54FB4B0BFD6AD93B9B9B01EE961E5@EXCHMBC1.ad.ah.local> Gary, I would strongly caution you not to pre-label your cassettes in big batches. You will be starting a process that will produce more errors than you can believe. "I'm a believer" in single piece flow. One at a time will greatly reduce numbering errors. Jan Omaha, NE ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Gary [gmartin@marshallmedical.org] Sent: Tuesday, April 01, 2008 2:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Labeling cassettes Our Pathologist presently label the cassettes at the time of grossing. We have decided it would be better for the someone else to do that task. We have a very small grossing area, and we will be labeling by hand. I am looking to the group for ideas concerning; 1) When are cassettes labeled ... when the specimens are stocked or at accessioning ... 2) How are the cassettes stored before the days grossing take place. Remembering we have a small area ... the table will need to be clear for frozen sections, etc. 3) Is there a resource for typical number of cassettes for particular specimens. The pathologists are having difficulty agreeing on that issue. Thanks as always Gary _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From victor <@t> pathology.washington.edu Tue Apr 1 15:23:32 2008 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Tue Apr 1 15:23:36 2008 Subject: [Histonet] RE: Labeling cassettes In-Reply-To: <346E5878979BA54FB4B0BFD6AD93B9B9B01EE961E5@EXCHMBC1.ad.ah.local> References: <6ED9D4252F278841A0593D3D788AF24C02159A81@mailsvr.MARSHMED.local> <346E5878979BA54FB4B0BFD6AD93B9B9B01EE961E5@EXCHMBC1.ad.ah.local> Message-ID: <47F299C4.3020909@pathology.washington.edu> Gary, I agree with Janice as we are moving away from pre-labeling. One of our programmers just wrote a custom software piece to barcode the specimen, from there whoever grosses will scan the specimen barcode and generate the cassettes immediately. We will be using the General Data cassette labeler which averages 4 seconds per cassette with barcode. The information will then be written to PowerPath our LIS. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Mahoney,Janice A wrote: > Gary, > I would strongly caution you not to pre-label your cassettes in big batches. You will be starting a process that will produce more errors than you can believe. "I'm a believer" in single piece flow. One at a time will greatly reduce numbering errors. > Jan > Omaha, NE > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Gary [gmartin@marshallmedical.org] > Sent: Tuesday, April 01, 2008 2:47 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Labeling cassettes > > Our Pathologist presently label the cassettes at the time of grossing. > We have decided it would be better for the someone else to do that task. > We have a very small grossing area, and we will be labeling by hand. I > am looking to the group for ideas concerning; > > 1) When are cassettes labeled ... when the specimens are > stocked or at accessioning ... > > 2) How are the cassettes stored before the days grossing > take place. Remembering we have a small area ... the table will need to > be clear for frozen sections, etc. > > 3) Is there a resource for typical number of cassettes > for particular specimens. The pathologists are having difficulty > agreeing on that issue. > > Thanks as always > > Gary > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. > > The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Barry.R.Rittman <@t> uth.tmc.edu Tue Apr 1 15:23:42 2008 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Tue Apr 1 15:23:48 2008 Subject: [Histonet] Democracy In-Reply-To: <5b6eb13e0804011237h60dbce82g14a15ec1ed7d0817@mail.gmail.com> References: <005701c89402$898bb8e0$6424d182@IBLS.GLA.AC.UK> <47F21D30.ACD8.0085.0@parkview.com> <000601c89411$a1662540$3d02a8c0@plab.local> <08A0A863637F1349BBFD83A96B27A50A120112@uwhis-xchng3.uwhis.hosp.wisc.edu> <5b6eb13e0804011237h60dbce82g14a15ec1ed7d0817@mail.gmail.com> Message-ID: Mark I was neither advocating for or against Canada Balsam. I don't believe that either could in the farthest reach of the imagination be classified as either religious or political. I did point out that there is in fact a place for both DPX and Canada Balsam. I have mounted ground sections in both DPX and others in Canada Balsam. I had fewer problems with bubbles and with air creeping in under the coverglass with Canada Balsam than with DPX. On the other hand if I had to mount several hundred slides I would prefer to use DPX chiefly because of the ease of cleaning the excess mountant from the slide. Too often in histology (as in life) everything is seen as pure black and white rather than shades of gray. Barry From: Mark Tarango [mailto:marktarango@gmail.com] Sent: Tuesday, April 01, 2008 2:38 PM To: Rittman, Barry R Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Democracy Barry- Wasn't it you, who just last week, was advocating for Canada Balsam?? Pretty hypocritical if you ask me. On Tue, Apr 1, 2008 at 11:55 AM, Rittman, Barry R wrote: Please, Histonet is not supposed to be a political or religious sounding board. If you wish to criticize this or any other country or any religion then please take this elsewhere. Let us stick to histology. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Tuesday, April 01, 2008 11:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Democracy Can't we just all play nice once in a while, instead of flying off the handle for one comment? OK, 2 or 3 would be a problem, but I am getting a bit tired of the vocal minority, on opposite ends of the spectrum. I think a part of the Democracy thing is that we should respect the idea of respect in freedom of speech. Sure people say all kinds of things, but we don't need to instantly attack them. We need to be respectful of others ideas and actions (as long as they aren't hurting anyone of course) even if we don't agree with them. They have their lives and let them live the way that makes them happy. (gee - pursuit of happiness...) I agree about the democratic liberties being taken for granted. I have visited over 15 countries in my youth and lived in Saudi Arabia for 4 years. I DO NOT take America for granted. I am even ashamed to call myself American on occasion (current administration comes to mind), but I am always grateful to have been born American to have the opportunities and quality of life I have. Opps, sorry I'll get off the soapbox now. Claire ________________________________ Go hang out in a Non-Democratic society for a few months then come back and tell us how you have never experienced it here in your homeland. You are clearly a product of a democratic nation; you take your liberties for granted so that you don't even know when you have them. If we don't like it we have the right to protest, to take action and change it! That's democracy. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Tue Apr 1 15:42:50 2008 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Tue Apr 1 15:42:57 2008 Subject: [Histonet] Alzheimer stain In-Reply-To: <47F2228F.4347.0054.0@ah.org> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273D3D@LSRIEXCH1.lsmaster.lifespan.org> In most techniques calling for a sodium salt, the equivalent potassium salt can be substituted. Though I am not familiar with the specific technique you are doing, I would try potassium carbonate in lieu of sodium carbonate. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Behnaz Sohrab > Sent: Tuesday, April 1, 2008 1:54 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Alzheimer stain > > Question regarding Sodium Carbonate Solution in Bielschowsky method for Alzheimer. Can I substitute Carbonate? Or any one has a better method for this stain? ( I do not have Sodium Carbonate, and no time to order it). > Thanks,Behnaz > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From awatanabe <@t> tgen.org Tue Apr 1 16:58:48 2008 From: awatanabe <@t> tgen.org (Aprill Watanabe) Date: Tue Apr 1 16:58:54 2008 Subject: [Histonet] Cytology on live culture cells Message-ID: Hello Histonetters, I need some help, I work in a research lab and a colleague is trying to differentiate live cells. I need a stain that we can do on live tissue culture cells to differentiate tumor cells from normal cells. These are cell lines being started from primary tumors. They know the population is mixed but needs a way to tell how pure the population is. They read an article about using DiffEQ or the Pap stain. I just don?t know how to do this in a flask or petri dish. I also am at a loss for which stain would stain which cell in those 2 stains. My other problem is that I have another colleague trying to stain live culture cells with cytokeratin AE1/AE3 from DAKO. We use MACH 3 mouse polymer kit from Biocare for the detection. She has tried it a couple of times and got no staining at all. She has tested the polymer kit and it works. She is using chamber slides to do the assay. She fixes with 4% PFA and does a tritonX digest. She has played with the concentration of the tritonX and still got nothing. She is setting up a few test experiments to test different fixatives and then to add some sort of retrieval step. The problem is we aren?t set up for anything other that autostainer retrieval or by pressure cooker, and we?re using chamber slides so I can?t put them on the autostainer. Any help/suggestions for either problem would be great. Thanks! Aprill Watanabe, B.S. Research Associate Integrated Cancer Genomics Division Tissue Microarray Center (TMA) Translational Genomics Research Institute (TGen) main: 602-343-8822 Fax: 602-343-8840 awatanabe@tgen.org www.tgen.org From ploykasek <@t> phenopath.com Tue Apr 1 17:15:23 2008 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Tue Apr 1 17:15:37 2008 Subject: [Histonet] RE: Labeling cassettes In-Reply-To: <47F299C4.3020909@pathology.washington.edu> Message-ID: Hi Victor. That sounds like a lovely system. I have a quick question. Are you printing out slides or slide labels, too? If so, what system are you using? Thanks. Patti Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > Gary, > > I agree with Janice as we are moving away from pre-labeling. One of our > programmers just wrote a custom software piece to barcode the specimen, > from there whoever grosses will scan the specimen barcode and generate > the cassettes immediately. We will be using the General Data cassette > labeler which averages 4 seconds per cassette with barcode. The > information will then be written to PowerPath our LIS. > > Victor > > Victor Tobias > Clinical Applications Analyst > University of Washington Medical Center > Dept of Pathology Room BB220 > 1959 NE Pacific > Seattle, WA 98195 > victor@pathology.washington.edu > 206-598-2792 > 206-598-7659 Fax > ================================================= > Privileged, confidential or patient identifiable information may be > contained in this message. This information is meant only for the use > of the intended recipients. If you are not the intended recipient, or > if the message has been addressed to you in error, do not read, > disclose, reproduce, distribute, disseminate or otherwise use this > transmission. Instead, please notify the sender by reply e-mail, and > then destroy all copies of the message and any attachments. > > > > Mahoney,Janice A wrote: >> Gary, >> I would strongly caution you not to pre-label your cassettes in big batches. >> You will be starting a process that will produce more errors than you can >> believe. "I'm a believer" in single piece flow. One at a time will greatly >> reduce numbering errors. >> Jan >> Omaha, NE >> >> ________________________________________ >> From: histonet-bounces@lists.utsouthwestern.edu >> [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Gary >> [gmartin@marshallmedical.org] >> Sent: Tuesday, April 01, 2008 2:47 PM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Labeling cassettes >> >> Our Pathologist presently label the cassettes at the time of grossing. >> We have decided it would be better for the someone else to do that task. >> We have a very small grossing area, and we will be labeling by hand. I >> am looking to the group for ideas concerning; >> >> 1) When are cassettes labeled ... when the specimens are >> stocked or at accessioning ... >> >> 2) How are the cassettes stored before the days grossing >> take place. Remembering we have a small area ... the table will need to >> be clear for frozen sections, etc. >> >> 3) Is there a resource for typical number of cassettes >> for particular specimens. The pathologists are having difficulty >> agreeing on that issue. >> >> Thanks as always >> >> Gary >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent >> Health is faithful to the healing ministry of Jesus Christ, providing high >> quality care for the body, mind and spirit of every person. >> >> The information contained in this communication, including attachments, is >> confidential and private and intended only for the use of the addressees. >> Unauthorized use, disclosure, distribution or copying is strictly prohibited >> and may be unlawful. If you received this communication in error, please >> inform us of the erroneous delivery by return e-mail message from your >> computer. Additionally, although all attachments have been scanned at the >> source for viruses, the recipient should check any attachments for the >> presence of viruses before opening. Alegent Health accepts no liability for >> any damage caused by any virus transmitted by this e-mail. Thank you for >> your cooperation. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From mickie25 <@t> netzero.net Tue Apr 1 17:14:14 2008 From: mickie25 <@t> netzero.net (mickie25@netzero.net) Date: Tue Apr 1 17:15:56 2008 Subject: [Histonet] Per diem position available in Cooperstown NY Message-ID: <20080401.181414.7310.6@webmail20.dca.untd.com> Hi Histonetters, I have a full time histology tech position available in Wisconsin. This is a M-F position with no evenings or weekends. They have a competitive reimbursement package including medical, 401K, and profit sharing. This is a growing Derm Path lab performing 25000 specimens a year potentially growing to over 50000 in the next two years. This is a great position with up to date equipment and performing special stains and immunohistochemistry too (automated). Please give me a call so I can give you more details. This is an opportunity to work in a great atmosphere with great fellow registered histotechs. Requires ASCP registry or eligible. Thank you. Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 www.mohshistologyconsulting.com -- "Spoon, Victoria" wrote: Return-Path: Received: from mx13.vgs.untd.com (mx13.vgs.untd.com [10.181.44.43]) by maildeliver02.dca.untd.com with SMTP id AABD9ES4SAFMGA6A for (sender ); Tue, 1 Apr 2008 07:14:40 -0700 (PST) Received: from swlx162.swmed.edu (swlx162.swmed.edu [199.165.152.162]) by mx13.vgs.untd.com with SMTP id AABD9ES4RAUAVQKJ for (sender ); Tue, 1 Apr 2008 07:14:39 -0700 (PST) Received: from localhost ([127.0.0.1] helo=swlx162.swmed.edu) by swlx162.swmed.edu with esmtp (Exim 4.34) id 1JghFU-000842-L1; Tue, 01 Apr 2008 09:14:04 -0500 Received: from [199.165.152.167] (helo=swlx167.swmed.edu) by swlx162.swmed.edu with esmtp (Exim 4.34) id 1JghFT-00083s-EZ for histonet@lists.utsouthwestern.edu; Tue, 01 Apr 2008 09:14:03 -0500 Received: from pathology.swmed.edu ([129.112.48.201]) by swlx167.swmed.edu with esmtp (Exim 4.44) id 1JghFS-0002zy-Pq for histonet@lists.utsouthwestern.edu; Tue, 01 Apr 2008 09:14:03 -0500 Received: from swlx160.swmed.edu (199.242.236.160) by pathology.swmed.edu with ESMTP (Eudora Internet Mail Server 3.1.5) for ; Tue, 1 Apr 2008 09:05:30 -0600 Received: from mail.bassett.org ([141.149.68.40] helo=screlay.bassett.org) by swlx160.swmed.edu with esmtp (Exim 4.69) (envelope-from ) id 1JghFQ-0008LB-F7 for histonet@pathology.swmed.edu; Tue, 01 Apr 2008 09:14:02 -0500 Received: from [175.25.43.88] by [175.25.43.75] with StormMail ESMTP id 42698.1293360943; Tue, 1 Apr 2008 10:19:20 -0500 (EST) X-MimeOLE: Produced By Microsoft Exchange V6.5 Content-class: urn:content-classes:message MIME-Version: 1.0 Date: Tue, 1 Apr 2008 10:11:18 -0400 Message-ID: <415700FC732DE14491A3E39367834F7701245A34@ex3.bassett.org> X-MS-Has-Attach: X-MS-TNEF-Correlator: Thread-Topic: Per diem position available in Cooperstown NY Thread-Index: AciUAkElonoH9HxtST6C/vy6cJ7dIw== From: "Spoon, Victoria" To: X-Scan-Signature: ce068603b9ccf40766a72bd9691ff331 X-Spam-Checker-Version: SpamAssassin 3.1.4 (2006-07-25) on swlx167.swmed.edu X-Spam-Level: X-Spam-Status: No, score=0.0 required=5.0 tests=HTML_MESSAGE, RCVD_DOUBLE_IP_LOOSE autolearn=unavailable version=3.1.4 Content-Type: text/plain; charset="us-ascii" Content-Transfer-Encoding: quoted-printable X-Content-Filtered-By: Mailman/MimeDel 2.1.5 Cc: Subject: [Histonet] Per diem position available in Cooperstown NY X-BeenThere: histonet@lists.utsouthwestern.edu X-Mailman-Version: 2.1.5 Precedence: list List-Id: For the exchange of information pertaining to histotechnology and related fields List-Unsubscribe: , List-Archive: List-Post: List-Help: List-Subscribe: , Sender: histonet-bounces@lists.utsouthwestern.edu Errors-To: histonet-bounces@lists.utsouthwestern.edu X-Scan-Signature: cf68ae42b5c1cad19b735e8c35ea8abb X-SA-Exim-Connect-IP: 127.0.0.1 X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu X-SA-Exim-Scanned: No (on swlx162.swmed.edu); SAEximRunCond expanded to false X-ContentStamp: 8:4:73707060 X-MAIL-INFO:1f59a95059f15050f16151c9617524d0d94481e9adad24e1e1e120c0c090f164f0f191f170ede0e0754549e1f16150c0513851c99994b13151b4 X-UNTD-Peer-Info: 199.165.152.162|swlx162.swmed.edu|swlx162.swmed.edu|histonet-bounces@lists.utsouthwestern.edu X-UNTD-UBE:-1 Bassett Hospital in Cooperstown NY, has a per diem histotechnician position available. Responsibilities include many aspects of histology (embedding, cutting, staining of human tissue) covering for coworkers time off. Applicants must possess an Associates degree and meet NYS licensure qualifications for a histotechnician. Bassett Hospital is a rural picturesque lakeside village, located in Upstate New York. Cooperstown offers year round cultural and recreational opportunities. Bassett employs 2000 employees in it's teaching and research system. Interested applicants can call Human Resources at (607)547-3120. or click on www.bassett.org NOTICE OF CONFIDENTIALITY This electronic message, including attachments, is for the sole use of the named recipient and may contain confidential or privileged information protected by New York State, and Federal regulations. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. If you are not the intended recipient or have received this communication in error please contact the sender or email.security@bassett.org and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pdunlop720 <@t> gmail.com Tue Apr 1 17:21:38 2008 From: pdunlop720 <@t> gmail.com (Patty Dunlop) Date: Tue Apr 1 17:21:42 2008 Subject: [Histonet] sections rolling - need ribbon! Message-ID: <80ab7bc60804011521w72b81147vc4fb9dbe6b5fad8e@mail.gmail.com> Hello Histonetters, We just opened a new lab and I am practicing on all the equipment this week to make sure everything is working properly before we go live. Having a problem - I can't get ribbons to form on the microtome. They keep rolling up on me and sometimes rise up with the block. I am using a brand new Leica RM 2255, and have tried clearance angles of 4, 5, and 6. Am using Accu-edge disposable blades, and am icing blocks before trying for sections. We are doing mostly small GI biopsies, so they are small blocks. Any suggestions would be appreciated! Thanks, Patty, HTL (ASCP) Monterey Bay GI Consultants From AnthonyH <@t> chw.edu.au Tue Apr 1 17:33:43 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Apr 1 17:34:56 2008 Subject: [Histonet] Blackballed In-Reply-To: <34BB307EFC9A65429BBB49E330675F720732EE6A@LTA3VS003.ees.hhs.gov> Message-ID: Jeanine, Why not? Unions in Australia were originally formed to protect the interests of workers. Their two main goals were worker's compensation and worker safety, and the right to be paid for overtime. Sometimes I wonder whether the unions have lost the plot, but overall they are a good counter-balance to the big corporations who are driven by shareholders' interests. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: Bartlett, Jeanine (CDC/CCID/NCZVED) [mailto:jqb7@cdc.gov] Sent: Tuesday, 1 April 2008 8:47 PM To: Tony Henwood; Steven Coakley; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Blackballed Not all of us have unions....... Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Monday, March 31, 2008 5:48 PM To: Steven Coakley; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Blackballed That's why we have unions. Firstly to ensure that we are all treated as humans!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven Coakley Sent: Tuesday, 1 April 2008 5:50 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Blackballed Well, I suppose I must accept that a certain Lab director has long ago blackballed me from working in Histology, at least, in my area. Guess that's what one gets from rebelling against being so stressed out from excessive periods of short staffing and temps. Working long hours, weekends, on call then being written up for taking advantage of the overtime. Oh well, tis life. I wish this individual great success in weeding out the bad Apple's as myself and others that just can't take the pressure. I hope she never succumbs to such an ordeal in her job. Signing off. You all stay strong and hang in there. No since continuing this membership. --------------------------------- Like movies? Here's a limited-time offer: Blockbuster Total Access for one month at no cost. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Tue Apr 1 17:40:04 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Apr 1 17:41:47 2008 Subject: FW: [Histonet] Blackballed In-Reply-To: <000601c89411$a1662540$3d02a8c0@plab.local> Message-ID: Whoa!! Hold on. The colonic polyp is about to hit the ceiling!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Wednesday, 2 April 2008 3:01 AM To: 'Bryan Watson'; ian.montgomery@bio.gla.ac.uk; histonet@lists.utsouthwestern.edu Subject: RE: FW: [Histonet] Blackballed Go hang out in a Non-Democratic society for a few months then come back and tell us how you have never experienced it here in your homeland. You are clearly a product of a democratic nation; you take your liberties for granted so that you don't even know when you have them. If we don't like it we have the right to protest, to take action and change it! That's democracy. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bryan Watson Sent: Tuesday, April 01, 2008 10:32 AM To: ian.montgomery@bio.gla.ac.uk; histonet@lists.utsouthwestern.edu Subject: Re: FW: [Histonet] Blackballed I've not been able to ever witness this alleged democracy myself. I've lived here my whole life. . . >>> "Ian Montgomery" 4/1/2008 10:13 >>> Surely in the World's greatest, allegedly, democracy you have employment laws that will protect workers from this type of behaviour? Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: 01 April 2008 10:47 To: Tony Henwood; Steven Coakley; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Blackballed Not all of us have unions....... Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Monday, March 31, 2008 5:48 PM To: Steven Coakley; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Blackballed That's why we have unions. Firstly to ensure that we are all treated as humans!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven Coakley Sent: Tuesday, 1 April 2008 5:50 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Blackballed Well, I suppose I must accept that a certain Lab director has long ago blackballed me from working in Histology, at least, in my area. Guess that's what one gets from rebelling against being so stressed out from excessive periods of short staffing and temps. Working long hours, weekends, on call then being written up for taking advantage of the overtime. Oh well, tis life. I wish this individual great success in weeding out the bad Apple's as myself and others that just can't take the pressure. I hope she never succumbs to such an ordeal in her job. Signing off. You all stay strong and hang in there. No since continuing this membership. --------------------------------- Like movies? Here's a limited-time offer: Blockbuster Total Access for one month at no cost. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From slappycraw <@t> yahoo.com Tue Apr 1 17:48:45 2008 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Tue Apr 1 17:48:50 2008 Subject: [Histonet] Blackballed In-Reply-To: <44780C571F28624DBB446DE55C4D733A1FE5A1@EXCHANGEBE1.carle.com> Message-ID: <78377.35730.qm@web53610.mail.re2.yahoo.com> The problem is that there are people in positions of power in this country not only in government but in the private sector as well, that are simply either incompetent, unable to handle the position properly, or they just plain don't do their job. It is clearly evident everywhere around in America today and there is plenty of blame to go around. Despite all of that, the US is still one of the best places to live unless one has unlimited resources to live where ever one wants to. "Charles.Embrey" wrote: Ian, I find your comment rather offensive and uncalled for. I hope you didn't mean it way it comes across. Charles Embrey, PA(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian Montgomery Sent: Tuesday, April 01, 2008 9:13 AM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] Blackballed Surely in the World's greatest, allegedly, democracy you have employment laws that will protect workers from this type of behaviour? Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: 01 April 2008 10:47 To: Tony Henwood; Steven Coakley; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Blackballed Not all of us have unions....... Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Monday, March 31, 2008 5:48 PM To: Steven Coakley; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Blackballed That's why we have unions. Firstly to ensure that we are all treated as humans!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven Coakley Sent: Tuesday, 1 April 2008 5:50 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Blackballed Well, I suppose I must accept that a certain Lab director has long ago blackballed me from working in Histology, at least, in my area. Guess that's what one gets from rebelling against being so stressed out from excessive periods of short staffing and temps. Working long hours, weekends, on call then being written up for taking advantage of the overtime. Oh well, tis life. I wish this individual great success in weeding out the bad Apple's as myself and others that just can't take the pressure. I hope she never succumbs to such an ordeal in her job. Signing off. You all stay strong and hang in there. No since continuing this membership. --------------------------------- Like movies? Here's a limited-time offer: Blockbuster Total Access for one month at no cost. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Larry A. Woody Seattle, Wa. --------------------------------- You rock. That's why Blockbuster's offering you one month of Blockbuster Total Access, No Cost. From amosbrooks <@t> gmail.com Tue Apr 1 18:09:09 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Tue Apr 1 18:09:13 2008 Subject: [Histonet] Blackballed Message-ID: <582736990804011609x2f9b8228n65e227ffb689d818@mail.gmail.com> OK, Now that is funny! I agree with the sentiment, but the tag line at the bottom left me in stitches. Stop talking about politics, let's talk about religion ... hehe! Amos Message: 8 Date: Tue, 1 Apr 2008 11:34:08 -0500 From: "Mahoney,Janice A" Subject: RE: [Histonet] Blackballed To: "histonet@lists.utsouthwestern.edu" Message-ID: <346E5878979BA54FB4B0BFD6AD93B9B9B01EE961DD@EXCHMBC1.ad.ah.local> Content-Type: text/plain; charset="us-ascii" Lets get back to our common vision and mission as Histotechnologists and do the best we can for science and patient care. Please quit with the political rhetoric and spewing of ideological opinions. We (the histonet) are an international, diverse group. That is part of what makes us so great. Life is very short and there's no time for fussing and fighting my friends! Jan Omaha, NE Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. From JMahoney <@t> alegent.org Tue Apr 1 18:28:57 2008 From: JMahoney <@t> alegent.org (Mahoney,Janice A) Date: Tue Apr 1 18:29:05 2008 Subject: [Histonet] Blackballed In-Reply-To: <582736990804011609x2f9b8228n65e227ffb689d818@mail.gmail.com> References: <582736990804011609x2f9b8228n65e227ffb689d818@mail.gmail.com> Message-ID: <346E5878979BA54FB4B0BFD6AD93B9B9B01EE961EE@EXCHMBC1.ad.ah.local> I know. It cracked me up too when I actually read my reply online. I can't get around the tag line. I should add something like "the tag line at the end of this e-mail does not reflect the feelings, thoughts, beliefs, reflections, sentiments, ideology or anything at all about the ambiguous writer." Jan ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks [amosbrooks@gmail.com] Sent: Tuesday, April 01, 2008 6:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blackballed OK, Now that is funny! I agree with the sentiment, but the tag line at the bottom left me in stitches. Stop talking about politics, let's talk about religion ... hehe! Amos Message: 8 Date: Tue, 1 Apr 2008 11:34:08 -0500 From: "Mahoney,Janice A" Subject: RE: [Histonet] Blackballed To: "histonet@lists.utsouthwestern.edu" Message-ID: <346E5878979BA54FB4B0BFD6AD93B9B9B01EE961DD@EXCHMBC1.ad.ah.local> Content-Type: text/plain; charset="us-ascii" Lets get back to our common vision and mission as Histotechnologists and do the best we can for science and patient care. Please quit with the political rhetoric and spewing of ideological opinions. We (the histonet) are an international, diverse group. That is part of what makes us so great. Life is very short and there's no time for fussing and fighting my friends! Jan Omaha, NE Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From Paul <@t> Firnschild.com Tue Apr 1 19:38:42 2008 From: Paul <@t> Firnschild.com (Paul Firnschild) Date: Tue Apr 1 18:38:48 2008 Subject: [Histonet] sections rolling - need ribbon! References: <80ab7bc60804011521w72b81147vc4fb9dbe6b5fad8e@mail.gmail.com> Message-ID: <058101c89459$e6ecd4e0$cf977e18@PhelpsDodge> It sounds like the retraction feature isn't working or is disabled. Consult the manual. Paul ----- Original Message ----- From: "Patty Dunlop" To: Sent: Tuesday, April 01, 2008 5:21 PM Subject: [Histonet] sections rolling - need ribbon! > Hello Histonetters, > > We just opened a new lab and I am practicing on all the equipment this week > to make sure everything is working properly before we go live. > Having a problem - I can't get ribbons to form on the microtome. They keep > rolling up on me and sometimes rise up with the block. I am using a brand > new Leica RM 2255, and have tried clearance angles of 4, 5, and 6. Am using > Accu-edge disposable blades, and am icing blocks before trying for > sections. We are doing mostly small GI biopsies, so they are small blocks. > > Any suggestions would be appreciated! > > Thanks, > Patty, HTL (ASCP) > Monterey Bay GI Consultants > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jnocito <@t> satx.rr.com Tue Apr 1 19:23:34 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Apr 1 19:23:07 2008 Subject: [Histonet] Democracy References: <005701c89402$898bb8e0$6424d182@IBLS.GLA.AC.UK><47F21D30.ACD8.0085.0@parkview.com><000601c89411$a1662540$3d02a8c0@plab.local><08A0A863637F1349BBFD83A96B27A50A120112@uwhis-xchng3.uwhis.hosp.wisc.edu> <5b6eb13e0804011237h60dbce82g14a15ec1ed7d0817@mail.gmail.com> Message-ID: <00dc01c89457$ca20ec40$0302a8c0@yourxhtr8hvc4p> ok, ok. Let's get to the real issues. If we don't have hematoxylin, can we use blueberry juice and vodka, or was than gin? ----- Original Message ----- From: "Mark Tarango" To: "Rittman, Barry R" Cc: Sent: Tuesday, April 01, 2008 2:37 PM Subject: Re: [Histonet] Democracy > Barry- > > Wasn't it you, who just last week, was advocating for Canada Balsam?? > Pretty hypocritical if you ask me. > > > > On Tue, Apr 1, 2008 at 11:55 AM, Rittman, Barry R < > Barry.R.Rittman@uth.tmc.edu> wrote: > >> Please, >> Histonet is not supposed to be a political or religious sounding board. >> If you wish to criticize this or any other country or any religion then >> please take this elsewhere. >> Let us stick to histology. >> Barry >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles >> Claire >> Sent: Tuesday, April 01, 2008 11:18 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Democracy >> >> Can't we just all play nice once in a while, instead of flying off the >> handle for one comment? OK, 2 or 3 would be a problem, but I am getting >> a bit tired of the vocal minority, on opposite ends of the spectrum. I >> think a part of the Democracy thing is that we should respect the idea >> of respect in freedom of speech. Sure people say all kinds of things, >> but we don't need to instantly attack them. We need to be respectful of >> others ideas and actions (as long as they aren't hurting anyone of >> course) even if we don't agree with them. They have their lives and let >> them live the way that makes them happy. (gee - pursuit of happiness...) >> I agree about the democratic liberties being taken for granted. I have >> visited over 15 countries in my youth and lived in Saudi Arabia for 4 >> years. I DO NOT take America for granted. I am even ashamed to call >> myself American on occasion (current administration comes to mind), but >> I am always grateful to have been born American to have the >> opportunities and quality of life I have. Opps, sorry I'll get off the >> soapbox now. >> >> Claire >> >> ________________________________ >> >> >> >> >> Go hang out in a Non-Democratic society for a few months then come back >> and >> tell us how you have never experienced it here in your homeland. You are >> clearly a product of a democratic nation; you take your liberties for >> granted so that you don't even know when you have them. If we don't like >> it >> we have the right to protest, to take action and change it! That's >> democracy. >> Cheryl Miller HT (ASCP) >> Histology Supervisor >> Physicians Laboratory,P.C. >> Omaha, Ne. >> 402 738 5052 >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JBower <@t> hei.org Tue Apr 1 19:31:17 2008 From: JBower <@t> hei.org (Bower, Jennifer) Date: Tue Apr 1 19:31:21 2008 Subject: [Histonet] Democracy In-Reply-To: <00dc01c89457$ca20ec40$0302a8c0@yourxhtr8hvc4p> References: <005701c89402$898bb8e0$6424d182@IBLS.GLA.AC.UK><47F21D30.ACD8.0085.0@parkview.com><000601c89411$a1662540$3d02a8c0@plab.local><08A0A863637F1349BBFD83A96B27A50A120112@uwhis-xchng3.uwhis.hosp.wisc.edu><5b6eb13e0804011237h60dbce82g14a15ec1ed7d0817@mail.gmail.com> <00dc01c89457$ca20ec40$0302a8c0@yourxhtr8hvc4p> Message-ID: <87449E4A2B01DA47B29424CE5D6E0F8307F86EBF@hi0sml1.hei.org> I figured it out! All day I've been working on this idea, and it works! All you have to do is run your slides through to water, then take a Sharpie pen (I use black) and ink all over the sample, then put the slides through the rest of the protocol, just replacing the hematoxylin step with the application of the Sharpie. I got wonderful blue/black nuclei and crisp chromatin detail. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Tuesday, April 01, 2008 5:24 PM To: Mark Tarango; Rittman, Barry R Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Democracy ok, ok. Let's get to the real issues. If we don't have hematoxylin, can we use blueberry juice and vodka, or was than gin? ----- Original Message ----- From: "Mark Tarango" To: "Rittman, Barry R" Cc: Sent: Tuesday, April 01, 2008 2:37 PM Subject: Re: [Histonet] Democracy > Barry- > > Wasn't it you, who just last week, was advocating for Canada Balsam?? > Pretty hypocritical if you ask me. > > > > On Tue, Apr 1, 2008 at 11:55 AM, Rittman, Barry R < > Barry.R.Rittman@uth.tmc.edu> wrote: > >> Please, >> Histonet is not supposed to be a political or religious sounding board. >> If you wish to criticize this or any other country or any religion then >> please take this elsewhere. >> Let us stick to histology. >> Barry >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles >> Claire >> Sent: Tuesday, April 01, 2008 11:18 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Democracy >> >> Can't we just all play nice once in a while, instead of flying off the >> handle for one comment? OK, 2 or 3 would be a problem, but I am getting >> a bit tired of the vocal minority, on opposite ends of the spectrum. I >> think a part of the Democracy thing is that we should respect the idea >> of respect in freedom of speech. Sure people say all kinds of things, >> but we don't need to instantly attack them. We need to be respectful of >> others ideas and actions (as long as they aren't hurting anyone of >> course) even if we don't agree with them. They have their lives and let >> them live the way that makes them happy. (gee - pursuit of happiness...) >> I agree about the democratic liberties being taken for granted. I have >> visited over 15 countries in my youth and lived in Saudi Arabia for 4 >> years. I DO NOT take America for granted. I am even ashamed to call >> myself American on occasion (current administration comes to mind), but >> I am always grateful to have been born American to have the >> opportunities and quality of life I have. Opps, sorry I'll get off the >> soapbox now. >> >> Claire >> >> ________________________________ >> >> >> >> >> Go hang out in a Non-Democratic society for a few months then come back >> and >> tell us how you have never experienced it here in your homeland. You are >> clearly a product of a democratic nation; you take your liberties for >> granted so that you don't even know when you have them. If we don't like >> it >> we have the right to protest, to take action and change it! That's >> democracy. >> Cheryl Miller HT (ASCP) >> Histology Supervisor >> Physicians Laboratory,P.C. >> Omaha, Ne. >> 402 738 5052 >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Wed Apr 2 04:43:25 2008 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Apr 2 04:43:35 2008 Subject: [Histonet] Hematoxylin Ripening Message-ID: <86ADE4EB583CE64799A9924684A0FBBF03CAE333@wahtntex2.waht.swest.nhs.uk> Kemlo - I didn't know there was enough sun there to ripen hematoxylin on a windowsill... :-) There was a window of opportunity between June and August usually; better since the potbanks closed down and the air cleared. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Don't be afraid to take a big step when one is indicated. You can't cross a chasm in two small jumps. --Buckminster Fuller This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From jqb7 <@t> cdc.gov Wed Apr 2 04:51:36 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed Apr 2 04:51:51 2008 Subject: [Histonet] Blackballed In-Reply-To: References: <34BB307EFC9A65429BBB49E330675F720732EE6A@LTA3VS003.ees.hhs.gov> Message-ID: <34BB307EFC9A65429BBB49E330675F720732EE86@LTA3VS003.ees.hhs.gov> In the position I hold here in the Federal Government there is no union. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Tuesday, April 01, 2008 6:34 PM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Steven Coakley; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Blackballed Jeanine, Why not? Unions in Australia were originally formed to protect the interests of workers. Their two main goals were worker's compensation and worker safety, and the right to be paid for overtime. Sometimes I wonder whether the unions have lost the plot, but overall they are a good counter-balance to the big corporations who are driven by shareholders' interests. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: Bartlett, Jeanine (CDC/CCID/NCZVED) [mailto:jqb7@cdc.gov] Sent: Tuesday, 1 April 2008 8:47 PM To: Tony Henwood; Steven Coakley; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Blackballed Not all of us have unions....... Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Monday, March 31, 2008 5:48 PM To: Steven Coakley; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Blackballed That's why we have unions. Firstly to ensure that we are all treated as humans!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven Coakley Sent: Tuesday, 1 April 2008 5:50 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Blackballed Well, I suppose I must accept that a certain Lab director has long ago blackballed me from working in Histology, at least, in my area. Guess that's what one gets from rebelling against being so stressed out from excessive periods of short staffing and temps. Working long hours, weekends, on call then being written up for taking advantage of the overtime. Oh well, tis life. I wish this individual great success in weeding out the bad Apple's as myself and others that just can't take the pressure. I hope she never succumbs to such an ordeal in her job. Signing off. You all stay strong and hang in there. No since continuing this membership. --------------------------------- Like movies? Here's a limited-time offer: Blockbuster Total Access for one month at no cost. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Wed Apr 2 04:54:03 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed Apr 2 04:54:19 2008 Subject: [Histonet] Democracy In-Reply-To: <00dc01c89457$ca20ec40$0302a8c0@yourxhtr8hvc4p> References: <005701c89402$898bb8e0$6424d182@IBLS.GLA.AC.UK><47F21D30.ACD8.0085.0@parkview.com><000601c89411$a1662540$3d02a8c0@plab.local><08A0A863637F1349BBFD83A96B27A50A120112@uwhis-xchng3.uwhis.hosp.wisc.edu> <5b6eb13e0804011237h60dbce82g14a15ec1ed7d0817@mail.gmail.com> <00dc01c89457$ca20ec40$0302a8c0@yourxhtr8hvc4p> Message-ID: <34BB307EFC9A65429BBB49E330675F720732EE87@LTA3VS003.ees.hhs.gov> Stoli blueberry vodka and blueberry juice..... Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Tuesday, April 01, 2008 8:24 PM To: Mark Tarango; Rittman, Barry R Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Democracy ok, ok. Let's get to the real issues. If we don't have hematoxylin, can we use blueberry juice and vodka, or was than gin? ----- Original Message ----- From: "Mark Tarango" To: "Rittman, Barry R" Cc: Sent: Tuesday, April 01, 2008 2:37 PM Subject: Re: [Histonet] Democracy > Barry- > > Wasn't it you, who just last week, was advocating for Canada Balsam?? > Pretty hypocritical if you ask me. > > > > On Tue, Apr 1, 2008 at 11:55 AM, Rittman, Barry R < > Barry.R.Rittman@uth.tmc.edu> wrote: > >> Please, >> Histonet is not supposed to be a political or religious sounding board. >> If you wish to criticize this or any other country or any religion then >> please take this elsewhere. >> Let us stick to histology. >> Barry >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles >> Claire >> Sent: Tuesday, April 01, 2008 11:18 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Democracy >> >> Can't we just all play nice once in a while, instead of flying off the >> handle for one comment? OK, 2 or 3 would be a problem, but I am getting >> a bit tired of the vocal minority, on opposite ends of the spectrum. I >> think a part of the Democracy thing is that we should respect the idea >> of respect in freedom of speech. Sure people say all kinds of things, >> but we don't need to instantly attack them. We need to be respectful of >> others ideas and actions (as long as they aren't hurting anyone of >> course) even if we don't agree with them. They have their lives and let >> them live the way that makes them happy. (gee - pursuit of happiness...) >> I agree about the democratic liberties being taken for granted. I have >> visited over 15 countries in my youth and lived in Saudi Arabia for 4 >> years. I DO NOT take America for granted. I am even ashamed to call >> myself American on occasion (current administration comes to mind), but >> I am always grateful to have been born American to have the >> opportunities and quality of life I have. Opps, sorry I'll get off the >> soapbox now. >> >> Claire >> >> ________________________________ >> >> >> >> >> Go hang out in a Non-Democratic society for a few months then come back >> and >> tell us how you have never experienced it here in your homeland. You are >> clearly a product of a democratic nation; you take your liberties for >> granted so that you don't even know when you have them. If we don't like >> it >> we have the right to protest, to take action and change it! That's >> democracy. >> Cheryl Miller HT (ASCP) >> Histology Supervisor >> Physicians Laboratory,P.C. >> Omaha, Ne. >> 402 738 5052 >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jfray80 <@t> hotmail.com Wed Apr 2 07:04:16 2008 From: jfray80 <@t> hotmail.com (JOSEPH FRAZEE) Date: Wed Apr 2 07:04:21 2008 Subject: [Histonet] Histology Assistant Message-ID: I am in search for a good histology assistant job description. If any one has one please share it with me .Thanks Histojoe. _________________________________________________________________ Get in touch in an instant. Get Windows Live Messenger now. http://www.windowslive.com/messenger/overview.html?ocid=TXT_TAGLM_WL_Refresh_getintouch_042008 From arvidsonkristen <@t> yahoo.com Wed Apr 2 07:12:08 2008 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Wed Apr 2 07:12:18 2008 Subject: [Histonet] Help-Fried tissue Message-ID: <733796.89990.qm@web65705.mail.ac4.yahoo.com> HELP!!! This is a very serious matter and we have tried everything. So I will get right to the point...we have had this artifact on and off for several years, the tissue looks shrunken on the slide and the Paths have a very hard time diagnosing. When embedding and cutting the pieces look smaller and are harder then their counter parts (sometimes one half of the specimen is fine while the other is destroyed in the same cassette!!). So here is what we do and what we have tried to remedy the situation.. we use recycled formalin, alcohol, and some clear-rite (BR instruments). We use multiple sized biopsy cassettes (mesh, small hole, and slotted--by the way we do all derm). We have tried shortening our processing times. We just purchased two new processors (Leica ASP300S) thinking that may have been the problem and the very first day we used them we had a large number of fried tissue. We also have two older VIP machines and it happens on and off on those as well. All our biopsies come in alcoholic formalin. We use paraplast. I think I've covered everything. We have come to some conclusions, we know that there could be air bubbles in the cassettes during processing and we've heard various things about the alcoholic formalin. So now what? Any other suggestions would be so helpful!! Thank you all for your time. -Kristen --------------------------------- You rock. That's why Blockbuster's offering you one month of Blockbuster Total Access, No Cost. From Jackie.O'Connor <@t> abbott.com Wed Apr 2 07:16:21 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed Apr 2 07:16:37 2008 Subject: [Histonet] Re: Illegal spam In-Reply-To: <87449E4A2B01DA47B29424CE5D6E0F8307F86EBF@hi0sml1.hei.org> Message-ID: I received an email from some moron calling himself "Bob Lama" about a free atm card - clearly an illegal scam asking me to send $600 for my $10milllion ATM card. I'm curious if this came from a histonet source, or should I look elsewhere for where this scam picked up my email address? Jackie O' From jennifer.l.hofecker <@t> Vanderbilt.Edu Wed Apr 2 07:32:10 2008 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Wed Apr 2 07:32:17 2008 Subject: [Histonet] Alzheimer stain In-Reply-To: <47F2228F.4347.0054.0@ah.org> Message-ID: <898D946569A27444B65667A49C0740520175B24D@mailbe06.mc.vanderbilt.edu> Good Morning, Behnaz. I am curious which modification of Bielschowsky you are using. Most of the common modifications do not require Sodium Carbonate. See below for my protocol which is based on Hirano's modified Bielschowsky. Please let me know if you have any additional questions. Good Luck! jennifer Jennifer L. Hofecker, HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph. (615)343-0083 fax. (615)343-7089 NSH Quality Control Committee Chair MODIFIED BIELSCHOWSKY METHOD SOLUTIONS/REAGENTS 1. 20% SILVER NITRATE Silver nitrate............20 g Distilled Water.........100 ml Mix well and protect from light. Prepare fresh. Fill one coplin jar and set aside. 2. AMMONIUM HYDROXIDE (CONCENTRATED) Commercially prepared 3. REDUCED SILVER SOLUTION Add ammonium hydroxide, drop by drop, with constant stirring to the remaining 20% silver nitrate solution. The solution will turn from dark brown to black and then to clear. Filter through #1 filter paper ALWAYS Prepare fresh. 4. AMMONIA WATER Ammonium hydroxide.........2 drops Distilled water......62.5ml Prepare fresh. 5. DEVELOPER (PREPARE IN THIS ORDER) Citric Acid.........0.25 g Distilled water......50ml Formaldehyde, 37%......10 ml Nitric acid...............25 uL Mix well after adding each item. Stable for 5 days @ room temp. 6. 5% SODIUM THIOSULFATE Sodium Thiosulfate...............5 g Distilled Water..................100 ml STAINING PROCEDURE 1. Deparaffinize sections and hydrate to distilled water. 2. Place slides 20% silver nitrate at room temp. for 30 min. Protect from light. 3. Rinse at least 6 times in millipore double distilled water. Save solution from step 4. Wipe moisture from slides and incubate in Reduced Silver solution. Protect from light. 5. Place slides directly in ammonia water. Save reduced silver. (Slides can remain in ammonia water for several minutes without harming the staining. Only 1-2 min are needed for the solution to have desired effects.) 6. Put 3 drops of developer in 30 ml of reduced silver (do not prepare earlier). 7. Place slides in this solution on a shaker near a microscope. 8. Watch for sections to turn brown. Rinse slides in water. Wipe back of slide and check under microscope. Plaques and tangles should be black. Check each slide individually. If not dark enough, return to ammonia water for 1 minute and back in reduced silver/developer solution. If silver starts to break down and form a "mirror" on the coplin jar, put 3 drops of developer in another 30 ml of reduced silver (can use solution from Step 4). 9. Wash in running water for 15 minutes. Rinse in distilled water. 10. Place in 5% sodium thiosulfate for 2 minutes. 11. Wash in running tap water for 1 minute. Rinse in distilled water. 12. Dehydrate through graded alcohols. 13. Clear in two changes of xylene, 3 min each. 14. Mount with synthetic resin. RESULTS Senile Plaques and neurofibrillary tangles of Alzheimer's disease - black Neurons and background - brown to yellow. Lipofuscin - brown or black -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Behnaz Sohrab Sent: Tuesday, April 01, 2008 1:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Alzheimer stain Question regarding Sodium Carbonate Solution in Bielschowsky method for Alzheimer. Can I substitute Carbonate? Or any one has a better method for this stain? ( I do not have Sodium Carbonate, and no time to order it). Thanks,Behnaz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ASPPagan <@t> aol.com Wed Apr 2 07:37:21 2008 From: ASPPagan <@t> aol.com (ASPPagan@aol.com) Date: Wed Apr 2 07:37:33 2008 Subject: [Histonet] Supplies for sale Message-ID: Hello Members, Our private lab has closed and we have some histo/cyto/immuno supplies that we must sell. Prices are negotiable. If you are interested please email me directly and I will forward a list of these items to you. Thanks _asppagan@aol.com_ (mailto:asppagan@aol.com) **************Create a Home Theater Like the Pros. Watch the video on AOL Home. (http://home.aol.com/diy/home-improvement-eric-stromer?video=15&ncid=aolhom00030000000001) From Kemlo.Rogerson <@t> waht.swest.nhs.uk Wed Apr 2 07:45:04 2008 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Apr 2 07:45:09 2008 Subject: [Histonet] Help-Fried tissue Message-ID: <86ADE4EB583CE64799A9924684A0FBBF03CAE33F@wahtntex2.waht.swest.nhs.uk> Don't panic Mr Mainwaring!!! You will need to do a little experimentation and only change one criteria at a time; I'd first look at fixation as it is the foundation for processing. You get brittle biopsies if you fix for long times in an alcoholic fixative for example Carnoys; you may wish to try something a tad gentler such as 10% aqueous buffered formalin. Either reduce the time in the alcoholic fixative or use the aqueous one then see what happens. If you are happy that it's not the fixative then look at the processing; too much time in the alcohols and clearing agents could cause brittleness. Make sure you have good, fresh solvents and minimise the dehydration times or reduce heat if you are using it. Finally look at the time you put the biopsies in paraffin wax and at what temperature. >From my experience brittleness is usually caused by (in order of commonness); Using an inappropriate fixative maybe for too long; or perversely too short a period and processing has an adverse effect on the unfixed proteins. Fixation provides a matrix by binding proteins together and allowing them to resist the strong coagulation effects of alcohol; if this doesn't occur then it's like trying to fix them in alcohol; it cause proteins to be strongly coagulated and a tendency towards brittleness. Too long, at too high a heat, in alcohols, clearing agent or wax. Usually if you get the fixation correct on small biopsies the rest follows, usually!!! The use of heat can cause proteins to become brittle by coagulating them and disrupting the matrix; this is exacerbated by heat. Try it in a systematic way, one thing at a time, see what happens; OK? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Don't be afraid to take a big step when one is indicated. You can't cross a chasm in two small jumps. --Buckminster Fuller This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From alaskagirl1950 <@t> yahoo.com Wed Apr 2 08:26:55 2008 From: alaskagirl1950 <@t> yahoo.com (Patricia Adams) Date: Wed Apr 2 08:27:02 2008 Subject: [Histonet] Democracy In-Reply-To: <87449E4A2B01DA47B29424CE5D6E0F8307F86EBF@hi0sml1.hei.org> Message-ID: <396975.24677.qm@web52510.mail.re2.yahoo.com> Before you let this out, we all need to take out stock in sharpie pens! --------------------------------- You rock. That's why Blockbuster's offering you one month of Blockbuster Total Access, No Cost. From shive003 <@t> umn.edu Wed Apr 2 09:18:08 2008 From: shive003 <@t> umn.edu (Jan Shivers) Date: Wed Apr 2 09:18:33 2008 Subject: Fw: [Histonet] Re: Illegal spam Message-ID: <004901c894cc$6030cfb0$b0065486@auxs.umn.edu> Ever since I subscribed to the Histonet, I've been receiving a lot of spam every day (mostly from people in Africa/Europe, either telling me I've won the lottery or asking me for my bank account number so that they can smuggle money out of their country). I can understand receiving this spam if this was a private home email account, with medium security levels, but I work at a University that has a very tight security system on their incoming email, so the only thing I can figure out is that this mail is getting through via a Histonet member's addressbook. I must have to delete 10-15 of these spams a day (it's the only spam I get, too). Very frustrating. Anybody have any suggestions as to how to eliminate these pesky creatures? Blocking the Sender doesn't work; they just change their addresses. Jan Shivers ----- Original Message ----- From: "Jackie M O'Connor" To: ; Sent: Wednesday, April 02, 2008 7:16 AM Subject: [Histonet] Re: Illegal spam >I received an email from some moron calling himself "Bob Lama" about a > free atm card - clearly an illegal scam asking me to send $600 for my > $10milllion ATM card. I'm curious if this came from a histonet source, or > should I look elsewhere for where this scam picked up my email address? > > Jackie O' > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rmweber113 <@t> comcast.net Wed Apr 2 09:31:07 2008 From: rmweber113 <@t> comcast.net (rmweber113@comcast.net) Date: Wed Apr 2 09:31:21 2008 Subject: [Histonet] 2 NJ JOB POSITIONS Message-ID: <040220081431.3630.47F398AB000DB8C400000E2E2215567074CCCECE9D0A0D0A99039D@comcast.net> I have 2 positions available in NJ. One is in Vorhees and the other is in Hillsborough. Both are part time, but Hillsborough can be made to full time. They are for a state of the art laboratory owned by private doctors practices. Hours are very flexible, can work around your schedule. Top pay offered. We also offer a $500 referral reward for candidates we hire and stay with us for at least 90 days. Interested candidates can email me their resume at rmweber113@comcast.net or call me at 732 814-1170 for further information. Thank you From erika.harrell <@t> teamstaffrx.com Wed Apr 2 09:42:47 2008 From: erika.harrell <@t> teamstaffrx.com (Erika Harrell) Date: Wed Apr 2 09:42:49 2008 Subject: [Histonet] Job available in Florida Message-ID: <19558353.1207147367308.JavaMail.cfservice@webserver32> Florida living at its best....within minutes to Gulf Beaches, Area Attractions, Shopping and every Sports Fan's dream! Position requires at least one year of experience in histologist techniques in hospital or reference histology laboratory. Licensed by the State of Florida as a Histotechnician or Histotechnologist. ASCP registry preferred. Full -time Permanent position and Hours are flexible! Relocation assistance is available. For more information, please contact Erika Harrell at 877.523.9897 ext 5475. Feel free to email your resume to erika.harrell@teamstaffrx.com or fax to 866.365.6566 Erika Harrell | Recruiter - Permanent Placement | TeamStaff Rx, Inc. Tel: 1.877.523.9897 ext 5475 | Fax: 1.866.365.6566 | www.teamstaffrx.com America?s Winning Healthcare Staffing Solution This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential or exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that you are strictly prohibited from printing, storing, disseminating, distributing or copying this communication. If you have received this communication in error, please notify the sender immediately by replying to the message and deleting it from your computer. Thank you. From Terry.Marshall <@t> rothgen.nhs.uk Wed Apr 2 09:51:18 2008 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Wed Apr 2 09:51:40 2008 Subject: [Histonet] Re: Illegal spam Message-ID: <5C0BED61F529364E86309CADEA63FEF20163F3B5@TRFT-EX01.xRothGen.nhs.uk> You are lucky to get that few. I get about 30 per day. Addresses are readily collected. There are trawlers available to do this. One of the spams I get every few months offers me something like 15 million e-mail addresses (for a tidy sum of course). Anti-spam programmes are not very efficient. I have tried several. Since spamming became illegal in the UK about 2 years ago, with monstrous fines, the result has been that nobody has been caught and spam has increased substantially. It's a single digit answer I'm afraid. Index finger and delete key:-( Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jan Shivers Sent: 02 April 2008 15:18 To: histonet Subject: Fw: [Histonet] Re: Illegal spam Ever since I subscribed to the Histonet, I've been receiving a lot of spam every day (mostly from people in Africa/Europe, either telling me I've won the lottery or asking me for my bank account number so that they can smuggle money out of their country). I can understand receiving this spam if this was a private home email account, with medium security levels, but I work at a University that has a very tight security system on their incoming email, so the only thing I can figure out is that this mail is getting through via a Histonet member's addressbook. I must have to delete 10-15 of these spams a day (it's the only spam I get, too). Very frustrating. Anybody have any suggestions as to how to eliminate these pesky creatures? Blocking the Sender doesn't work; they just change their addresses. Jan Shivers ----- Original Message ----- From: "Jackie M O'Connor" To: ; Sent: Wednesday, April 02, 2008 7:16 AM Subject: [Histonet] Re: Illegal spam >I received an email from some moron calling himself "Bob Lama" about a >free atm card - clearly an illegal scam asking me to send $600 for my >$10milllion ATM card. I'm curious if this came from a histonet source, >or should I look elsewhere for where this scam picked up my email address? > > Jackie O' > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mickie25 <@t> netzero.net Wed Apr 2 09:51:16 2008 From: mickie25 <@t> netzero.net (mickie25@netzero.net) Date: Wed Apr 2 09:53:51 2008 Subject: Fw: [Histonet] Re: Illegal spam Message-ID: <20080402.105116.21462.1@webmail20.dca.untd.com> Jan, I have been a member of Histonet for over a year now and I have never received but one or two emails like you describe and some of those were before I signed up for histonet. Your University spam filter may not be working? Good Luck. Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 www.mohshistologyconsulting.com -- "Jan Shivers" wrote: Return-Path: Received: from mx03.vgs.untd.com (mx03.vgs.untd.com [10.181.44.33]) by maildeliver05.dca.untd.com with SMTP id AABD9HFRJA564V2S for (sender ); Wed, 2 Apr 2008 07:19:21 -0700 (PST) Received: from swlx162.swmed.edu (swlx162.swmed.edu [199.165.152.162]) by mx03.vgs.untd.com with SMTP id AABD9HFRJAJZFFLS for (sender ); Wed, 2 Apr 2008 07:19:20 -0700 (PST) Received: from localhost ([127.0.0.1] helo=swlx162.swmed.edu) by swlx162.swmed.edu with esmtp (Exim 4.34) id 1Jh3nO-0007jw-0b; Wed, 02 Apr 2008 09:18:34 -0500 Received: from [199.242.236.161] (helo=swlx161.swmed.edu) by swlx162.swmed.edu with esmtp (Exim 4.34) id 1Jh3nM-0007jr-AP for histonet@lists.utsouthwestern.edu; Wed, 02 Apr 2008 09:18:32 -0500 Received: from mta-w2.tc.umn.edu ([134.84.119.6]) by swlx161.swmed.edu with esmtp (Exim 4.69) (envelope-from ) id 1Jh3nH-0001yH-R2 for histonet@lists.utsouthwestern.edu; Wed, 02 Apr 2008 09:18:32 -0500 Received: from vdltemp1 (x-134-84-6-176.cvm.umn.edu [134.84.6.176]) by mta-w2.tc.umn.edu (UMN smtpd) with ESMTP for ; Wed, 2 Apr 2008 09:18:08 -0500 (CDT) X-Umn-Remote-Mta: [N] x-134-84-6-176.cvm.umn.edu [134.84.6.176] #+LO+TS+AU+HN Message-ID: <004901c894cc$6030cfb0$b0065486@auxs.umn.edu> From: "Jan Shivers" To: "histonet" Date: Wed, 2 Apr 2008 09:18:08 -0500 MIME-Version: 1.0 Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Content-Transfer-Encoding: 7bit X-Priority: 3 X-MSMail-Priority: Normal X-Mailer: Microsoft Outlook Express 6.00.2900.3138 X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 X-Scan-Signature: 301c769310e5573f5c674635cd2c198a X-Spam-Checker-Version: SpamAssassin 3.2.4 (2008-01-01) on swlx161.swmed.edu X-Spam-Level: X-Spam-Status: No, score=-4.0 required=5.0 tests=RCVD_IN_DNSWL_MED, STOX_REPLY_TYPE autolearn=disabled version=3.2.4 X-Spam-Relay-Country: US US Subject: Fw: [Histonet] Re: Illegal spam X-BeenThere: histonet@lists.utsouthwestern.edu X-Mailman-Version: 2.1.5 Precedence: list List-Id: For the exchange of information pertaining to histotechnology and related fields List-Unsubscribe: , List-Archive: List-Post: List-Help: List-Subscribe: , Sender: histonet-bounces@lists.utsouthwestern.edu Errors-To: histonet-bounces@lists.utsouthwestern.edu X-Scan-Signature: 91c5be5f340ec53dc388915214e1bad9 X-SA-Exim-Connect-IP: 127.0.0.1 X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu X-SA-Exim-Scanned: No (on swlx162.swmed.edu); SAEximRunCond expanded to false X-ContentStamp: 12:6:1012525019 X-MAIL-INFO:04cdbc11cd751111759df5289dad95a8d52900a52c2c95818181e13d3d49753ced75f9753571b8b8adf10581759d113df55df528d9385cddf5dc X-UNTD-Peer-Info: 199.165.152.162|swlx162.swmed.edu|swlx162.swmed.edu|histonet-bounces@lists.utsouthwestern.edu X-UNTD-UBE:-1 Ever since I subscribed to the Histonet, I've been receiving a lot of spam every day (mostly from people in Africa/Europe, either telling me I've won the lottery or asking me for my bank account number so that they can smuggle money out of their country). I can understand receiving this spam if this was a private home email account, with medium security levels, but I work at a University that has a very tight security system on their incoming email, so the only thing I can figure out is that this mail is getting through via a Histonet member's addressbook. I must have to delete 10-15 of these spams a day (it's the only spam I get, too). Very frustrating. Anybody have any suggestions as to how to eliminate these pesky creatures? Blocking the Sender doesn't work; they just change their addresses. Jan Shivers ----- Original Message ----- From: "Jackie M O'Connor" To: ; Sent: Wednesday, April 02, 2008 7:16 AM Subject: [Histonet] Re: Illegal spam >I received an email from some moron calling himself "Bob Lama" about a > free atm card - clearly an illegal scam asking me to send $600 for my > $10milllion ATM card. I'm curious if this came from a histonet source, or > should I look elsewhere for where this scam picked up my email address? > > Jackie O' > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Wed Apr 2 10:02:06 2008 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Wed Apr 2 10:02:27 2008 Subject: Fw: [Histonet] Re: Illegal spam Message-ID: <5C0BED61F529364E86309CADEA63FEF20163F3B6@TRFT-EX01.xRothGen.nhs.uk> Well, that stuff below your signature is worse than any previous spam I've had. LOL Odd how experiences can be so different too. I guess it's a matter of luck whether you get caught in the address trawl. Whether corporate spam filters are better I doubt. We have just had a trial of one, and it picked up about 5%. (Plus another 10% that was not spam). Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mickie25@netzero.net Sent: 02 April 2008 15:51 To: shive003@umn.edu Cc: histonet@lists.utsouthwestern.edu Subject: Re: Fw: [Histonet] Re: Illegal spam Jan, I have been a member of Histonet for over a year now and I have never received but one or two emails like you describe and some of those were before I signed up for histonet. Your University spam filter may not be working? Good Luck. Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 www.mohshistologyconsulting.com -- "Jan Shivers" wrote: Return-Path: Received: from mx03.vgs.untd.com (mx03.vgs.untd.com [10.181.44.33]) by maildeliver05.dca.untd.com with SMTP id AABD9HFRJA564V2S for (sender ); Wed, 2 Apr 2008 07:19:21 -0700 (PST) Received: from swlx162.swmed.edu (swlx162.swmed.edu [199.165.152.162]) by mx03.vgs.untd.com with SMTP id AABD9HFRJAJZFFLS for (sender ); Wed, 2 Apr 2008 07:19:20 -0700 (PST) Received: from localhost ([127.0.0.1] helo=swlx162.swmed.edu) by swlx162.swmed.edu with esmtp (Exim 4.34) id 1Jh3nO-0007jw-0b; Wed, 02 Apr 2008 09:18:34 -0500 Received: from [199.242.236.161] (helo=swlx161.swmed.edu) by swlx162.swmed.edu with esmtp (Exim 4.34) id 1Jh3nM-0007jr-AP for histonet@lists.utsouthwestern.edu; Wed, 02 Apr 2008 09:18:32 -0500 Received: from mta-w2.tc.umn.edu ([134.84.119.6]) by swlx161.swmed.edu with esmtp (Exim 4.69) (envelope-from ) id 1Jh3nH-0001yH-R2 for histonet@lists.utsouthwestern.edu; Wed, 02 Apr 2008 09:18:32 -0500 Received: from vdltemp1 (x-134-84-6-176.cvm.umn.edu [134.84.6.176]) by mta-w2.tc.umn.edu (UMN smtpd) with ESMTP for ; Wed, 2 Apr 2008 09:18:08 -0500 (CDT) X-Umn-Remote-Mta: [N] x-134-84-6-176.cvm.umn.edu [134.84.6.176] #+LO+TS+AU+HN Message-ID: <004901c894cc$6030cfb0$b0065486@auxs.umn.edu> From: "Jan Shivers" To: "histonet" Date: Wed, 2 Apr 2008 09:18:08 -0500 MIME-Version: 1.0 Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Content-Transfer-Encoding: 7bit X-Priority: 3 X-MSMail-Priority: Normal X-Mailer: Microsoft Outlook Express 6.00.2900.3138 X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 X-Scan-Signature: 301c769310e5573f5c674635cd2c198a X-Spam-Checker-Version: SpamAssassin 3.2.4 (2008-01-01) on swlx161.swmed.edu X-Spam-Level: X-Spam-Status: No, score=-4.0 required=5.0 tests=RCVD_IN_DNSWL_MED, STOX_REPLY_TYPE autolearn=disabled version=3.2.4 X-Spam-Relay-Country: US US Subject: Fw: [Histonet] Re: Illegal spam X-BeenThere: histonet@lists.utsouthwestern.edu X-Mailman-Version: 2.1.5 Precedence: list List-Id: For the exchange of information pertaining to histotechnology and related fields List-Unsubscribe: , List-Archive: List-Post: List-Help: List-Subscribe: , Sender: histonet-bounces@lists.utsouthwestern.edu Errors-To: histonet-bounces@lists.utsouthwestern.edu X-Scan-Signature: 91c5be5f340ec53dc388915214e1bad9 X-SA-Exim-Connect-IP: 127.0.0.1 X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu X-SA-Exim-Scanned: No (on swlx162.swmed.edu); SAEximRunCond expanded to false X-ContentStamp: 12:6:1012525019 X-MAIL-INFO:04cdbc11cd751111759df5289dad95a8d52900a52c2c95818181e13d3d49 753ced75f9753571b8b8adf10581759d113df55df528d9385cddf5dc X-UNTD-Peer-Info: 199.165.152.162|swlx162.swmed.edu|swlx162.swmed.edu|histonet-bounces@lis ts.utsouthwestern.edu X-UNTD-UBE:-1 Ever since I subscribed to the Histonet, I've been receiving a lot of spam every day (mostly from people in Africa/Europe, either telling me I've won the lottery or asking me for my bank account number so that they can smuggle money out of their country). I can understand receiving this spam if this was a private home email account, with medium security levels, but I work at a University that has a very tight security system on their incoming email, so the only thing I can figure out is that this mail is getting through via a Histonet member's addressbook. I must have to delete 10-15 of these spams a day (it's the only spam I get, too). Very frustrating. Anybody have any suggestions as to how to eliminate these pesky creatures? Blocking the Sender doesn't work; they just change their addresses. Jan Shivers ----- Original Message ----- From: "Jackie M O'Connor" To: ; Sent: Wednesday, April 02, 2008 7:16 AM Subject: [Histonet] Re: Illegal spam >I received an email from some moron calling himself "Bob Lama" about a >free atm card - clearly an illegal scam asking me to send $600 for my >$10milllion ATM card. I'm curious if this came from a histonet source, >or should I look elsewhere for where this scam picked up my email address? > > Jackie O' > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pmarcum <@t> vet.upenn.edu Wed Apr 2 10:02:30 2008 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Wed Apr 2 10:02:41 2008 Subject: [Histonet] Goat Collagen I and II HELP Message-ID: <6.2.5.6.2.20080402105446.01c91498@vet.upenn.edu> Hi All, I was hoping some one on our lists has successfully gotten collagen staining on goat bone. I have tried five different companies for antibodies and none are working on either EDTA decalcified goat bone or MMA embedded and de- plasticized goat bone. These are goat knees with the cartilage still attached and some collagen in a defect. have also tried three different secondary kits with no luck. One was an Alkaline Phosphatase Kit and two DAB kits with universal secondaries. Please help if you can or tell me it can't be done so I can get some rest. Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From m5johnso <@t> ucsd.edu Wed Apr 2 10:24:10 2008 From: m5johnso <@t> ucsd.edu (Johnson, Mindy) Date: Wed Apr 2 10:24:51 2008 Subject: Fw: [Histonet] Re: Illegal spam In-Reply-To: <5C0BED61F529364E86309CADEA63FEF20163F3B6@TRFT-EX01.xRothGen.nhs.uk> References: <5C0BED61F529364E86309CADEA63FEF20163F3B6@TRFT-EX01.xRothGen.nhs.uk> Message-ID: Just so you know, I work at a university also and I get these spam emails, BUT my started before signing up with Histonet. Spammers are very good at targeting Universities because once they figure out how the university assigns email address it's just a matter of going through a list. So, I wouldn't blame histonet. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr, Consultant Histopathologist Sent: Wednesday, April 02, 2008 8:02 AM To: mickie25@netzero.net; shive003@umn.edu Cc: histonet@lists.utsouthwestern.edu Subject: RE: Fw: [Histonet] Re: Illegal spam Well, that stuff below your signature is worse than any previous spam I've had. LOL Odd how experiences can be so different too. I guess it's a matter of luck whether you get caught in the address trawl. Whether corporate spam filters are better I doubt. We have just had a trial of one, and it picked up about 5%. (Plus another 10% that was not spam). Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mickie25@netzero.net Sent: 02 April 2008 15:51 To: shive003@umn.edu Cc: histonet@lists.utsouthwestern.edu Subject: Re: Fw: [Histonet] Re: Illegal spam Jan, I have been a member of Histonet for over a year now and I have never received but one or two emails like you describe and some of those were before I signed up for histonet. Your University spam filter may not be working? Good Luck. Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 www.mohshistologyconsulting.com -- "Jan Shivers" wrote: Return-Path: Received: from mx03.vgs.untd.com (mx03.vgs.untd.com [10.181.44.33]) by maildeliver05.dca.untd.com with SMTP id AABD9HFRJA564V2S for (sender ); Wed, 2 Apr 2008 07:19:21 -0700 (PST) Received: from swlx162.swmed.edu (swlx162.swmed.edu [199.165.152.162]) by mx03.vgs.untd.com with SMTP id AABD9HFRJAJZFFLS for (sender ); Wed, 2 Apr 2008 07:19:20 -0700 (PST) Received: from localhost ([127.0.0.1] helo=swlx162.swmed.edu) by swlx162.swmed.edu with esmtp (Exim 4.34) id 1Jh3nO-0007jw-0b; Wed, 02 Apr 2008 09:18:34 -0500 Received: from [199.242.236.161] (helo=swlx161.swmed.edu) by swlx162.swmed.edu with esmtp (Exim 4.34) id 1Jh3nM-0007jr-AP for histonet@lists.utsouthwestern.edu; Wed, 02 Apr 2008 09:18:32 -0500 Received: from mta-w2.tc.umn.edu ([134.84.119.6]) by swlx161.swmed.edu with esmtp (Exim 4.69) (envelope-from ) id 1Jh3nH-0001yH-R2 for histonet@lists.utsouthwestern.edu; Wed, 02 Apr 2008 09:18:32 -0500 Received: from vdltemp1 (x-134-84-6-176.cvm.umn.edu [134.84.6.176]) by mta-w2.tc.umn.edu (UMN smtpd) with ESMTP for ; Wed, 2 Apr 2008 09:18:08 -0500 (CDT) X-Umn-Remote-Mta: [N] x-134-84-6-176.cvm.umn.edu [134.84.6.176] #+LO+TS+AU+HN Message-ID: <004901c894cc$6030cfb0$b0065486@auxs.umn.edu> From: "Jan Shivers" To: "histonet" Date: Wed, 2 Apr 2008 09:18:08 -0500 MIME-Version: 1.0 Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Content-Transfer-Encoding: 7bit X-Priority: 3 X-MSMail-Priority: Normal X-Mailer: Microsoft Outlook Express 6.00.2900.3138 X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 X-Scan-Signature: 301c769310e5573f5c674635cd2c198a X-Spam-Checker-Version: SpamAssassin 3.2.4 (2008-01-01) on swlx161.swmed.edu X-Spam-Level: X-Spam-Status: No, score=-4.0 required=5.0 tests=RCVD_IN_DNSWL_MED, STOX_REPLY_TYPE autolearn=disabled version=3.2.4 X-Spam-Relay-Country: US US Subject: Fw: [Histonet] Re: Illegal spam X-BeenThere: histonet@lists.utsouthwestern.edu X-Mailman-Version: 2.1.5 Precedence: list List-Id: For the exchange of information pertaining to histotechnology and related fields List-Unsubscribe: , List-Archive: List-Post: List-Help: List-Subscribe: , Sender: histonet-bounces@lists.utsouthwestern.edu Errors-To: histonet-bounces@lists.utsouthwestern.edu X-Scan-Signature: 91c5be5f340ec53dc388915214e1bad9 X-SA-Exim-Connect-IP: 127.0.0.1 X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu X-SA-Exim-Scanned: No (on swlx162.swmed.edu); SAEximRunCond expanded to false X-ContentStamp: 12:6:1012525019 X-MAIL-INFO:04cdbc11cd751111759df5289dad95a8d52900a52c2c95818181e13d3d49 753ced75f9753571b8b8adf10581759d113df55df528d9385cddf5dc X-UNTD-Peer-Info: 199.165.152.162|swlx162.swmed.edu|swlx162.swmed.edu|histonet-bounces@lis ts.utsouthwestern.edu X-UNTD-UBE:-1 Ever since I subscribed to the Histonet, I've been receiving a lot of spam every day (mostly from people in Africa/Europe, either telling me I've won the lottery or asking me for my bank account number so that they can smuggle money out of their country). I can understand receiving this spam if this was a private home email account, with medium security levels, but I work at a University that has a very tight security system on their incoming email, so the only thing I can figure out is that this mail is getting through via a Histonet member's addressbook. I must have to delete 10-15 of these spams a day (it's the only spam I get, too). Very frustrating. Anybody have any suggestions as to how to eliminate these pesky creatures? Blocking the Sender doesn't work; they just change their addresses. Jan Shivers ----- Original Message ----- From: "Jackie M O'Connor" To: ; Sent: Wednesday, April 02, 2008 7:16 AM Subject: [Histonet] Re: Illegal spam >I received an email from some moron calling himself "Bob Lama" about a >free atm card - clearly an illegal scam asking me to send $600 for my >$10milllion ATM card. I'm curious if this came from a histonet source, >or should I look elsewhere for where this scam picked up my email address? > > Jackie O' > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SohrabB1 <@t> ah.org Wed Apr 2 10:49:30 2008 From: SohrabB1 <@t> ah.org (Behnaz Sohrab) Date: Wed Apr 2 10:50:14 2008 Subject: [Histonet] Amazing Message-ID: <47F34895.4347.0054.0@ah.org> Wonderful. Thanks, you all are amazingly dependable. Thank you all, Behnaz From Terry.Marshall <@t> rothgen.nhs.uk Wed Apr 2 10:55:31 2008 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Wed Apr 2 10:55:42 2008 Subject: [Histonet] Amazing Message-ID: <5C0BED61F529364E86309CADEA63FEF20163F3B9@TRFT-EX01.xRothGen.nhs.uk> Huh? About or in relation to what? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Behnaz Sohrab Sent: 02 April 2008 16:50 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Amazing Wonderful. Thanks, you all are amazingly dependable. Thank you all, Behnaz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From timothy.macatee <@t> med.nyu.edu Wed Apr 2 11:05:53 2008 From: timothy.macatee <@t> med.nyu.edu (Tim Macatee) Date: Wed Apr 2 11:08:41 2008 Subject: [Histonet] Re: Illegal spam In-Reply-To: Message-ID: Our email software actually put this email into my bulk mail folder, while the rest of the emails from this string went directly to my inbox. I wonder why? Tim On 4/2/08 11:24 AM, "Johnson, Mindy" wrote: > Just so you know, I work at a university also and I get these spam emails, BUT > my started before signing up with Histonet. Spammers are very good at > targeting Universities because once they figure out how the university assigns > email address it's just a matter of going through a list. So, I wouldn't > blame histonet. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry > Dr, Consultant Histopathologist > Sent: Wednesday, April 02, 2008 8:02 AM > To: mickie25@netzero.net; shive003@umn.edu > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: Fw: [Histonet] Re: Illegal spam > > Well, that stuff below your signature is worse than any previous spam > I've had. > LOL > > Odd how experiences can be so different too. I guess it's a matter of > luck whether you get caught in the address trawl. > Whether corporate spam filters are better I doubt. We have just had a > trial of one, and it picked up about 5%. (Plus another 10% that was not > spam). > > Terry > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > mickie25@netzero.net > Sent: 02 April 2008 15:51 > To: shive003@umn.edu > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: Fw: [Histonet] Re: Illegal spam > > Jan, > I have been a member of Histonet for over a year now and I have never > received but one or two emails like you describe and some of those were > before I signed up for histonet. Your University spam filter may not be > working? Good Luck. > Mickie > > > Mickie Johnson, B.S., HTL(ASCP) > Mohs Histology Consulting Services > 2507 S. Manito Blvd. > Spokane, WA 99203 > 509-954-7134 www.mohshistologyconsulting.com > > -- "Jan Shivers" wrote: > Return-Path: > Received: from mx03.vgs.untd.com (mx03.vgs.untd.com [10.181.44.33]) by > maildeliver05.dca.untd.com with SMTP id AABD9HFRJA564V2S for > (sender > ); > Wed, 2 Apr 2008 07:19:21 -0700 (PST) > Received: from swlx162.swmed.edu (swlx162.swmed.edu [199.165.152.162]) > by mx03.vgs.untd.com with SMTP id AABD9HFRJAJZFFLS for > (sender > ); > Wed, 2 Apr 2008 07:19:20 -0700 (PST) > Received: from localhost ([127.0.0.1] helo=swlx162.swmed.edu) by > swlx162.swmed.edu with esmtp (Exim 4.34) id 1Jh3nO-0007jw-0b; Wed, 02 > Apr 2008 09:18:34 -0500 > Received: from [199.242.236.161] (helo=swlx161.swmed.edu) by > swlx162.swmed.edu with esmtp (Exim 4.34) id 1Jh3nM-0007jr-AP for > histonet@lists.utsouthwestern.edu; Wed, 02 Apr 2008 09:18:32 -0500 > Received: from mta-w2.tc.umn.edu ([134.84.119.6]) by swlx161.swmed.edu > with esmtp (Exim 4.69) (envelope-from ) id > 1Jh3nH-0001yH-R2 for histonet@lists.utsouthwestern.edu; Wed, 02 Apr 2008 > 09:18:32 -0500 > Received: from vdltemp1 (x-134-84-6-176.cvm.umn.edu [134.84.6.176]) by > mta-w2.tc.umn.edu (UMN smtpd) with ESMTP for > ; > Wed, 2 Apr 2008 09:18:08 -0500 (CDT) > X-Umn-Remote-Mta: [N] x-134-84-6-176.cvm.umn.edu [134.84.6.176] > #+LO+TS+AU+HN > Message-ID: <004901c894cc$6030cfb0$b0065486@auxs.umn.edu> > From: "Jan Shivers" > To: "histonet" > Date: Wed, 2 Apr 2008 09:18:08 -0500 > MIME-Version: 1.0 > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > Content-Transfer-Encoding: 7bit > X-Priority: 3 > X-MSMail-Priority: Normal > X-Mailer: Microsoft Outlook Express 6.00.2900.3138 > X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 > X-Scan-Signature: 301c769310e5573f5c674635cd2c198a > X-Spam-Checker-Version: SpamAssassin 3.2.4 (2008-01-01) on > swlx161.swmed.edu > X-Spam-Level: > X-Spam-Status: No, score=-4.0 required=5.0 tests=RCVD_IN_DNSWL_MED, > STOX_REPLY_TYPE autolearn=disabled version=3.2.4 > X-Spam-Relay-Country: US US > Subject: Fw: [Histonet] Re: Illegal spam > X-BeenThere: histonet@lists.utsouthwestern.edu > X-Mailman-Version: 2.1.5 > Precedence: list > List-Id: For the exchange of information pertaining to histotechnology > and related fields > List-Unsubscribe: > , > > List-Archive: > List-Post: > List-Help: > > List-Subscribe: > , > > Sender: histonet-bounces@lists.utsouthwestern.edu > Errors-To: histonet-bounces@lists.utsouthwestern.edu > X-Scan-Signature: 91c5be5f340ec53dc388915214e1bad9 > X-SA-Exim-Connect-IP: 127.0.0.1 > X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu > X-SA-Exim-Scanned: No (on swlx162.swmed.edu); SAEximRunCond expanded to > false > X-ContentStamp: 12:6:1012525019 > X-MAIL-INFO:04cdbc11cd751111759df5289dad95a8d52900a52c2c95818181e13d3d49 > 753ced75f9753571b8b8adf10581759d113df55df528d9385cddf5dc > X-UNTD-Peer-Info: > 199.165.152.162|swlx162.swmed.edu|swlx162.swmed.edu|histonet-bounces@lis > ts.utsouthwestern.edu > X-UNTD-UBE:-1 > Ever since I subscribed to the Histonet, I've been receiving a lot of > spam every day (mostly from people in Africa/Europe, either telling me > I've won the lottery or asking me for my bank account number so that > they can smuggle money out of their country). > > I can understand receiving this spam if this was a private home email > account, with medium security levels, but I work at a University that > has a very tight security system on their incoming email, so the only > thing I can figure out is that this mail is getting through via a > Histonet member's addressbook. > > I must have to delete 10-15 of these spams a day (it's the only spam I > get, too). Very frustrating. Anybody have any suggestions as to how to > eliminate these pesky creatures? Blocking the Sender doesn't work; they > just change their addresses. > > Jan Shivers > > ----- Original Message ----- > From: "Jackie M O'Connor" > To: ; > > Sent: Wednesday, April 02, 2008 7:16 AM > Subject: [Histonet] Re: Illegal spam > > >> I received an email from some moron calling himself "Bob Lama" about a > >> free atm card - clearly an illegal scam asking me to send $600 for my >> $10milllion ATM card. I'm curious if this came from a histonet source, > >> or should I look elsewhere for where this scam picked up my email > address? >> >> Jackie O' >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. 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The organization accepts no liability for any damage caused by any virus transmitted by this email. ================================= From mcauliff <@t> umdnj.edu Wed Apr 2 11:28:20 2008 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Apr 2 11:28:20 2008 Subject: [Histonet] Sharpie Ink staining In-Reply-To: <87449E4A2B01DA47B29424CE5D6E0F8307F86EBF@hi0sml1.hei.org> References: <005701c89402$898bb8e0$6424d182@IBLS.GLA.AC.UK> <47F21D30.ACD8.0085.0@parkview.com> <000601c89411$a1662540$3d02a8c0@plab.local> <08A0A863637F1349BBFD83A96B27A50A120112@uwhis-xchng3.uwhis.hosp.wisc.edu> <5b6eb13e0804011237h60dbce82g14a15ec1ed7d0817@mail.gmail.com> <00dc01c89457$ca20ec40$0302a8c0@yourxhtr8hvc4p> <87449E4A2B01DA47B29424CE5D6E0F8307F86EBF@hi0sml1.hei.org> Message-ID: <47F3B424.60705@umdnj.edu> I noticed this years ago. I labeled a slide with Sharpie ink. The alcohol, or was it xylene, level in the Coplin jar was high enough to dissolve the ink. Voila, stained nuclei! I guess I should have published it. Geoff Bower, Jennifer wrote: > I figured it out! All day I've been working on this idea, and it works! > All you have to do is run your slides through to water, then take a > Sharpie pen (I use black) and ink all over the sample, then put the > slides through the rest of the protocol, just replacing the hematoxylin > step with the application of the Sharpie. I got wonderful blue/black > nuclei and crisp chromatin detail. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe > Nocito > Sent: Tuesday, April 01, 2008 5:24 PM > To: Mark Tarango; Rittman, Barry R > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Democracy > > ok, ok. Let's get to the real issues. > If we don't have hematoxylin, can we use blueberry juice and vodka, or > was > than gin? > > -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From mwich <@t> 7thwavelabs.com Wed Apr 2 12:28:19 2008 From: mwich <@t> 7thwavelabs.com (Michele Wich) Date: Wed Apr 2 12:28:24 2008 Subject: [Histonet] luxol fast blue/NFR Message-ID: <2264717ADC396742A0FF0AAB674F9A0D486C24@7THWAVE-SERVER.7thwave.local> Has anyone ever done a Luxol Fast Blue stain with a nuclear fast red counterstain? Does anyone know what would be an advantage of using this over cresyl echt violet? And lastly, if someone is using NFR as a counterstain, is it necessary to heat it as is done with the CEV when counterstaining? Any info is greatly appreciated! This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer From jkiernan <@t> uwo.ca Wed Apr 2 12:35:23 2008 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Apr 2 12:36:25 2008 Subject: Fw: [Histonet] Re: Illegal spam In-Reply-To: References: <5C0BED61F529364E86309CADEA63FEF20163F3B6@TRFT-EX01.xRothGen.nhs.uk> Message-ID: Jan Shivers wrote: > . . . at a university . . . > . . .I must have to delete 10-15 of these spams a day . . . You're lucky to get so few! At my university email address I have to check through about 50 filtered (probable spam) titles each day in addition to deleting 10 or so that make it into the Inbox. John Kiernan Anatomy, UWO London, Canada = = = From kayd <@t> cytolabpathology.com Wed Apr 2 13:31:56 2008 From: kayd <@t> cytolabpathology.com (Kay Duffy) Date: Wed Apr 2 13:31:57 2008 Subject: [Histonet] (no subject) Message-ID: <147174A7C9FDD344822BD4D944B5589E2E3F3E@08CPSMX.cytolabpathology.com> Please cancel my membership and take me off this List! ASAP Kay Duffy 4/2/08 From jkiernan <@t> uwo.ca Wed Apr 2 13:49:27 2008 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Apr 2 13:49:34 2008 Subject: [Histonet] (no subject) In-Reply-To: <147174A7C9FDD344822BD4D944B5589E2E3F3E@08CPSMX.cytolabpathology.com> References: <147174A7C9FDD344822BD4D944B5589E2E3F3E@08CPSMX.cytolabpathology.com> Message-ID: It's not that easy! You need to visit the web site: [1]http://lists.utsouthwestern.edu/mailman/listinfo/histonet Its address appears at the end of every Histonet message. - - John Kiernan Anatomy, UWO London, Canada = = = From: Kay Duffy [2]kayd@cytolabpathology.com wrote: > Please cancel my membership and take me off this List! > > ASAP > > Kay Duffy References 1. http://lists.utsouthwestern.edu/mailman/listinfo/histonet 2. mailto:kayd@cytolabpathology.com From Dorothy.L.Webb <@t> HealthPartners.Com Wed Apr 2 13:51:49 2008 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Wed Apr 2 13:51:58 2008 Subject: [Histonet] Could you post this for fellow histologist?? Message-ID: <0E394B648E5284478A6CCB78E5AFDA27056355E0@hpes1.HealthPartners.int> HISTO TECHNOLOGIST Seeking highly qualified and experienced Histo Technologist to relocate to the beautiful Black Hills of South Dakota. Candidate would join our Dermatology Department and start the Histo Laboratory along side our existing Mohs Lab. Excellent salary and benefits package. Clinic Environment. No Call - Flexible work hours available. Apply to: Human Resources Rapid City Medical Center, LLP 2820 Mt. Rushmore Road Rapid City, SD 57701 (605) 342-3280 Fax (605) 721-8458 Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From rjbuesa <@t> yahoo.com Wed Apr 2 14:41:18 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Apr 2 14:41:25 2008 Subject: [Histonet] sections rolling - need ribbon! In-Reply-To: <80ab7bc60804011521w72b81147vc4fb9dbe6b5fad8e@mail.gmail.com> Message-ID: <291129.82467.qm@web65713.mail.ac4.yahoo.com> Check the type of paraffin, it may be of a very low melting point. Check also the thickness you have set the microtome for, it may be too thick. Ren? J. Patty Dunlop wrote: Hello Histonetters, We just opened a new lab and I am practicing on all the equipment this week to make sure everything is working properly before we go live. Having a problem - I can't get ribbons to form on the microtome. They keep rolling up on me and sometimes rise up with the block. I am using a brand new Leica RM 2255, and have tried clearance angles of 4, 5, and 6. Am using Accu-edge disposable blades, and am icing blocks before trying for sections. We are doing mostly small GI biopsies, so they are small blocks. Any suggestions would be appreciated! Thanks, Patty, HTL (ASCP) Monterey Bay GI Consultants _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- You rock. That's why Blockbuster's offering you one month of Blockbuster Total Access, No Cost. From drkwolfe <@t> telus.net Wed Apr 2 16:14:32 2008 From: drkwolfe <@t> telus.net (drkwolfe@telus.net) Date: Wed Apr 2 16:14:40 2008 Subject: [Histonet] Re: Illegal spam In-Reply-To: References: Message-ID: <1207170872.47f3f738e2207@webmail.telus.net> In response to Jackie O's concern and to respond to some of the comments already raised on this issue (if I am repeating what someone else has now already stated I appologize, I only scanned the first half-dozen or so). Histonet is NOT responible for giving out anyone's e-mail address. They have a fine reputation, and I seriously doubt anyone would mess with it. Any web site on this wide open world of the internet is accessible to most anyone. Even secure websites can be hacked. The persons behind most of the spam we receive use robots (computer program) to search the internet for anyone that has posted their email address in a visible manner (such as the archived material on Histonet.org contains email addresses in posts and responses). These robots then report on the list of E-Mail addresses its harvested. Those e- mail addresses are added to a cataloge that is made available to anyone willing to spend a few dollars for it. Spammers take these lists and use them for spamming, and some for more profitable ventures. So to be on the safe side... never openly post your email address on a website. or if you do, do so in a manner that does not automatically respond as an e- mail address (yourname (space) @ (space) Yourprovider.org or by simply waiting to e-mail your address to someone in a more private medium (messenger, chat, etc) I hope that is of some help. And hopefully it will save someone the headache of massive spam in the future (when I ran my own gaming website, I would receive on average of 500-1000 spam e-mails a day as all website E-Mails were directed to myself [editor, owner, webmaster, etc]). Thanks All and Have a great day Joe Kapler Quoting Jackie M O'Connor : > I received an email from some moron calling himself "Bob Lama" about a > free atm card - clearly an illegal scam asking me to send $600 for my > $10milllion ATM card. I'm curious if this came from a histonet source, or > should I look elsewhere for where this scam picked up my email address? > > Jackie O' > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From sccrshlly <@t> yahoo.com Wed Apr 2 16:35:54 2008 From: sccrshlly <@t> yahoo.com (Shelly Coker) Date: Wed Apr 2 16:36:02 2008 Subject: [Histonet] Re: Fried Tissue Message-ID: <636006.88700.qm@web90306.mail.mud.yahoo.com> Kristen, Because the problem is so random, have you explored the possibility that perhaps the problem is occurring prior to processing? We were having some problems with hard, shrunken tissue, and it turns out that the grosser was allowing the tissue to dry out before placing the cassette in formalin because it was "faster" :( . This could also happen at the point of collection. How does the tissue look under the microscope? You could ask your pathologist if the artifact could possibly be air-drying artifact. Good luck, Shelly --------------------------------- You rock. That's why Blockbuster's offering you one month of Blockbuster Total Access, No Cost. From gmartin <@t> marshallmedical.org Wed Apr 2 16:57:39 2008 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Wed Apr 2 16:57:49 2008 Subject: [Histonet] Cassette Labeling Message-ID: <6ED9D4252F278841A0593D3D788AF24C02193C59@mailsvr.MARSHMED.local> Thanks to all who responded to my questions about cassette labeling. We have decided to; 1) label the cassettes as we stock the accessioned specimens. 2) store the labeled cassettes in designed bins the we discovered through this list. These storage bins are sold by Hacker Instruments 3) I will ... as suggested, and as we have done ... review the number of cassettes typically used for particular specimens and create a list that will indicate the # of cassettes for that specimen. If the Pathologist uses or needs more ... they will have to create those extra cassettes. Again thank you very much for the help. Gary From jnocito <@t> satx.rr.com Wed Apr 2 18:17:15 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Apr 2 18:16:49 2008 Subject: [Histonet] Help-Fried tissue References: <733796.89990.qm@web65705.mail.ac4.yahoo.com> Message-ID: <001801c89517$b0e80040$0302a8c0@yourxhtr8hvc4p> Kristin, what temps are your paraffin baths set at? Is there enough solutions to cover all the blocks? Are you putting in fresh alcohols and clear-rite in the last stations, or is every container on the processors filled with recycled reagents? Are you flaming your forceps when embedding? These are some questions just off the top of my toes, I mean head JTT ----- Original Message ----- From: "kristen arvidson" To: "histonet" Sent: Wednesday, April 02, 2008 7:12 AM Subject: [Histonet] Help-Fried tissue > HELP!!! > > This is a very serious matter and we have tried everything. So I will > get right to the point...we have had this artifact on and off for several > years, the tissue looks shrunken on the slide and the Paths have a very > hard time diagnosing. When embedding and cutting the pieces look smaller > and are harder then their counter parts (sometimes one half of the > specimen is fine while the other is destroyed in the same cassette!!). > > So here is what we do and what we have tried to remedy the situation.. > we use recycled formalin, alcohol, and some clear-rite (BR instruments). > We use multiple sized biopsy cassettes (mesh, small hole, and slotted--by > the way we do all derm). We have tried shortening our processing times. > We just purchased two new processors (Leica ASP300S) thinking that may > have been the problem and the very first day we used them we had a large > number of fried tissue. We also have two older VIP machines and it > happens on and off on those as well. All our biopsies come in alcoholic > formalin. We use paraplast. I think I've covered everything. We have > come to some conclusions, we know that there could be air bubbles in the > cassettes during processing and we've heard various things about the > alcoholic formalin. So now what? Any other suggestions would be so > helpful!! Thank you all for your time. > > -Kristen > > > --------------------------------- > You rock. That's why Blockbuster's offering you one month of Blockbuster > Total Access, No Cost. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histology.bc <@t> shaw.ca Wed Apr 2 18:50:01 2008 From: histology.bc <@t> shaw.ca (Paul Bradbury) Date: Wed Apr 2 18:45:53 2008 Subject: [Histonet] luxol fast blue/NFR In-Reply-To: <2264717ADC396742A0FF0AAB674F9A0D486C24@7THWAVE-SERVER.7thwave.local> References: <2264717ADC396742A0FF0AAB674F9A0D486C24@7THWAVE-SERVER.7thwave.local> Message-ID: <47F41BA9.7040409@shaw.ca> The obvious advantage to using nuclear fast red is the contrast between the phthalocyanin blue of the myelin and the clear red shades of the chromatin. Cresyl echt violet is a purple-blue that does not contrast optimally with the blue of the myelin. Both counterstains will show Nissl granules as desired, but small granules may be more difficult to see when stained red as opposed to when they are stained purple-blue. Nuclear fast red will stain very well at room temperature ... no need to heat it. A 0.5% aqueous solution of NFR will produce nice results with staining times of 5-10 minutes. Some of the stain will be removed by the dehydrating alcohols, so fairly quick dehydration is better. Paul Bradbury Kamloops, Canada Michele Wich wrote: > Has anyone ever done a Luxol Fast Blue stain with a nuclear fast red > counterstain? Does anyone know what would be an advantage of using this > over cresyl echt violet? And lastly, if someone is using NFR as a > counterstain, is it necessary to heat it as is done with the CEV when > counterstaining? > > Any info is greatly appreciated! > > > This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From amosbrooks <@t> gmail.com Wed Apr 2 19:34:27 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed Apr 2 21:14:28 2008 Subject: [Histonet] Re: Illegal spam Message-ID: <582736990804021734t77eda02bk5fa5f17892846a5a@mail.gmail.com> Hi, I also have a University email that has very tight security. I do not use that for the Histonet as I subscribe from home. My University email account is also deluged with spam. I do not think the Histonet really has anything to do with spam messages. The best advise I can offer is to filter messages based on the subject line rather than the sender. Filtering messages with "Viagara", "V1agara", "Cialis" and "Cia1is" will be much more helpful. Amos Message: 17 Date: Wed, 2 Apr 2008 09:18:08 -0500 From: "Jan Shivers" Subject: Fw: [Histonet] Re: Illegal spam To: "histonet" Message-ID: <004901c894cc$6030cfb0$b0065486@auxs.umn.edu> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Ever since I subscribed to the Histonet, I've been receiving a lot of spam every day (mostly from people in Africa/Europe, either telling me I've won the lottery or asking me for my bank account number so that they can smuggle money out of their country). I can understand receiving this spam if this was a private home email account, with medium security levels, but I work at a University that has a very tight security system on their incoming email, so the only thing I can figure out is that this mail is getting through via a Histonet member's addressbook. I must have to delete 10-15 of these spams a day (it's the only spam I get, too). Very frustrating. Anybody have any suggestions as to how to eliminate these pesky creatures? Blocking the Sender doesn't work; they just change their addresses. Jan Shivers From Tony_Reilly <@t> health.qld.gov.au Wed Apr 2 23:30:50 2008 From: Tony_Reilly <@t> health.qld.gov.au (Anthony Reilly) Date: Wed Apr 2 23:31:10 2008 Subject: [Histonet] Help-Fried tissue In-Reply-To: <733796.89990.qm@web65705.mail.ac4.yahoo.com> References: <733796.89990.qm@web65705.mail.ac4.yahoo.com> Message-ID: <47F4EA0F.471C.0039.0@health.qld.gov.au> Hi Kristen I would certainly look at replacing the alcoholic formalin with an aqueous solution as over exposure to alcohol will harden your tissue unnecessarily however I do not think that this is the main problem. Your tissue has dried at some point and the fact that some samples have a mixture of good and bad areas would suggest it is during processing. Other than ensuring that solution levels are adequate as previously suggested I would look at the biopsy cassettes and how they are stacked in the processor. When using biopsy cassettes with a fine mesh there is marked reduction of the flow of solutions which will be worsened by stacking the cassettes too tightly in processor racks. If you have not done so already I would suggest trying paper wraps (we use end papers from hairdressing suppliers) in routine cassettes which are loosely stacked into the processing racks. Some have racks with spacers. just a suggestion. Good luck. regards Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_reilly@health.qld.gov.au >>> kristen arvidson 2/04/2008 10:12 pm >>> HELP!!! This is a very serious matter and we have tried everything. So I will get right to the point...we have had this artifact on and off for several years, the tissue looks shrunken on the slide and the Paths have a very hard time diagnosing. When embedding and cutting the pieces look smaller and are harder then their counter parts (sometimes one half of the specimen is fine while the other is destroyed in the same cassette!!). So here is what we do and what we have tried to remedy the situation.. we use recycled formalin, alcohol, and some clear-rite (BR instruments). We use multiple sized biopsy cassettes (mesh, small hole, and slotted--by the way we do all derm). We have tried shortening our processing times. We just purchased two new processors (Leica ASP300S) thinking that may have been the problem and the very first day we used them we had a large number of fried tissue. We also have two older VIP machines and it happens on and off on those as well. All our biopsies come in alcoholic formalin. We use paraplast. I think I've covered everything. We have come to some conclusions, we know that there could be air bubbles in the cassettes during processing and we've heard various things about the alcoholic formalin. So now what? Any other suggestions would be so helpful!! Thank you all for your time. -Kristen --------------------------------- You rock. That's why Blockbuster's offering you one month of Blockbuster Total Access, No Cost. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/ received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. **************************************************************** From djamesnz <@t> orcon.net.nz Thu Apr 3 01:03:21 2008 From: djamesnz <@t> orcon.net.nz (Darren James) Date: Thu Apr 3 01:39:01 2008 Subject: [Histonet] Re: Illegal spam In-Reply-To: <582736990804021734t77eda02bk5fa5f17892846a5a@mail.gmail.com> References: <582736990804021734t77eda02bk5fa5f17892846a5a@mail.gmail.com> Message-ID: Spammers also use programmes which basically create algorithms of common email address configurations ie @hotmail.com, @gmail.com etc and send these out without knowing if they have a correct or valid address. Despite the temptation to reply with an insulting response (as I have done in a fit of frustration), never reply to these emails. It only confirms to the spammer that they have found a viable email address. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Thursday, 3 April 2008 1:34 p.m. To: shive003@umn.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Illegal spam Hi, I also have a University email that has very tight security. I do not use that for the Histonet as I subscribe from home. My University email account is also deluged with spam. I do not think the Histonet really has anything to do with spam messages. The best advise I can offer is to filter messages based on the subject line rather than the sender. Filtering messages with "Viagara", "V1agara", "Cialis" and "Cia1is" will be much more helpful. Amos Message: 17 Date: Wed, 2 Apr 2008 09:18:08 -0500 From: "Jan Shivers" Subject: Fw: [Histonet] Re: Illegal spam To: "histonet" Message-ID: <004901c894cc$6030cfb0$b0065486@auxs.umn.edu> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Ever since I subscribed to the Histonet, I've been receiving a lot of spam every day (mostly from people in Africa/Europe, either telling me I've won the lottery or asking me for my bank account number so that they can smuggle money out of their country). I can understand receiving this spam if this was a private home email account, with medium security levels, but I work at a University that has a very tight security system on their incoming email, so the only thing I can figure out is that this mail is getting through via a Histonet member's addressbook. I must have to delete 10-15 of these spams a day (it's the only spam I get, too). Very frustrating. Anybody have any suggestions as to how to eliminate these pesky creatures? Blocking the Sender doesn't work; they just change their addresses. Jan Shivers _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG. Version: 7.5.519 / Virus Database: 269.22.4/1355 - Release Date: 1/04/2008 5:37 p.m. No virus found in this outgoing message. Checked by AVG. Version: 7.5.519 / Virus Database: 269.22.4/1355 - Release Date: 1/04/2008 5:37 p.m. From gu.lang <@t> gmx.at Thu Apr 3 02:23:03 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Apr 3 02:23:12 2008 Subject: AW: [Histonet] sections rolling - need ribbon! In-Reply-To: <291129.82467.qm@web65713.mail.ac4.yahoo.com> Message-ID: <000501c8955b$8e9f3460$eeeea8c0@dielangs.at> Hi Ren?, perhaps I have a mess in my brain and you can help me out. I always thought, that paraffin with a low melting point is softer at roomtemperature. And therefore would be stickier. So my opinion is: high melting point = harder, ribbons are more difficult. Low melting point = softer, sections are sticky and ribbons are easier. You know, I am a sliding microtome user. So I could be wrong. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Rene J Buesa Gesendet: Mittwoch, 02. April 2008 21:41 An: Patty Dunlop; histonet@lists.utsouthwestern.edu Betreff: Re: [Histonet] sections rolling - need ribbon! Check the type of paraffin, it may be of a very low melting point. Check also the thickness you have set the microtome for, it may be too thick. Ren? J. Patty Dunlop wrote: Hello Histonetters, We just opened a new lab and I am practicing on all the equipment this week to make sure everything is working properly before we go live. Having a problem - I can't get ribbons to form on the microtome. They keep rolling up on me and sometimes rise up with the block. I am using a brand new Leica RM 2255, and have tried clearance angles of 4, 5, and 6. Am using Accu-edge disposable blades, and am icing blocks before trying for sections. We are doing mostly small GI biopsies, so they are small blocks. Any suggestions would be appreciated! Thanks, Patty, HTL (ASCP) Monterey Bay GI Consultants _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- You rock. That's why Blockbuster's offering you one month of Blockbuster Total Access, No Cost. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joost.bruijntjes <@t> tno.nl Thu Apr 3 02:49:52 2008 From: joost.bruijntjes <@t> tno.nl (Bruijntjes, J.P. (Joost)) Date: Thu Apr 3 02:50:27 2008 Subject: [Histonet] neuronal Message-ID: <8865601DD17A554CB489C17FFD8A51B201164207@MAIL04.tsn.tno.nl> Hi Histonetters Is anyone of you familiar with the application of antibodies like A2B5, Nestin of NG2? These antibodies can be found on precursor/stem cells, which differentiate into neuronal cells. Any help is appreciated. Joost Bruijntjes TNO Quality of Life Zeist The Netherlands TNO.NL Joost Bruijntjes T +31 30 694 44 80 F +31 30 694 49 86 E joost.bruijntjes@tno.nl Disclaimer This e-mail and its contents are subject to the DISCLAIMER at http://www.tno.nl/disclaimer/email.html From nefff <@t> staff.uni-marburg.de Thu Apr 3 04:14:58 2008 From: nefff <@t> staff.uni-marburg.de (Dr. med. Frauke Neff) Date: Thu Apr 3 04:15:08 2008 Subject: [Histonet] F4/80 Message-ID: <1207214098.47f4a0122c09a@webmail.med.uni-marburg.de> Dear colleagues, is anybody there who has experiences with a F4/80 antibody? It is supposed to label "activated" microglia. Unfortunatly I don't get a specific staining in mice. Does anyone have an idea? Thanks a lot, F. Neff ---------------------------------------------------------------- This message was sent using IMP, the Internet Messaging Program. From Yves.Heremans <@t> vub.ac.be Thu Apr 3 05:10:02 2008 From: Yves.Heremans <@t> vub.ac.be (Yves Heremans) Date: Thu Apr 3 05:10:19 2008 Subject: [Histonet] granules after X-gal staining Message-ID: <4A400492-BBAC-4D00-9021-6E74F59996E9@vub.ac.be> Dear Histonetters, Does anyone know why I am getting granules (tiny, round blue granules in the cytoplasm) after X-gal staining on frozen sections of mouse pancreas ? Regards, Yves From Terry.Marshall <@t> rothgen.nhs.uk Thu Apr 3 06:13:41 2008 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Thu Apr 3 06:13:58 2008 Subject: [Histonet] Re: Illegal spam Message-ID: <5C0BED61F529364E86309CADEA63FEF20163F3BD@TRFT-EX01.xRothGen.nhs.uk> So John is inundated too. Tough John, but nice to hear from you again - it seems rare nowadays. At the risk of stretching this thread, in-between 2 of my post yesterday, I had two adverts for a bigger erection, and this morning one of these adverts of which I spoke, offering lists. This is a MD's list. See below. Board Certified MDs in the USA 788,865 in total <> 17,817 emails Featuring the most complete contact information in many different areas of medicine Over a dozen sortable fields Price for this week only = $398 +++ You get these for F-Ree with every order this week +++ -> American Pharmaceutical Company Contact List Personal email addresses (47,000 in total) and names for top level executives -> Hospitals in the US more than 23k hospital administrators in over 7k hospitals [worth over $300 alone) -> Extensive Directory of Dentists in the United States Practically every dentist in the USA is listed here -> US Chiropractor List Over than 100k chiropractors practicing in the United States send and email to: leslie.carroll_dr@hotmail.com this offer is only valid until April 4 2008 and email with 309 in the subject will ensure no further communication from us Terry ________________________________ From: John Kiernan [mailto:jkiernan@uwo.ca] Sent: 02 April 2008 18:35 To: Johnson, Mindy Cc: Marshall Terry Dr, Consultant Histopathologist; mickie25@netzero.net; shive003@umn.edu; histonet@lists.utsouthwestern.edu Subject: Re: RE: Fw: [Histonet] Re: Illegal spam Jan Shivers wrote: > . . . at a university . . . > . . .I must have to delete 10-15 of these spams a day . . . You're lucky to get so few! At my university email address I have to check through about 50 filtered (probable spam) titles each day in addition to deleting 10 or so that make it into the Inbox. John Kiernan Anatomy, UWO London, Canada = = = From jnocito <@t> satx.rr.com Thu Apr 3 06:17:16 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Apr 3 06:16:50 2008 Subject: [Histonet] chewing gum Message-ID: <000801c8957c$468f5120$0302a8c0@yourxhtr8hvc4p> I know I'm going to feel stupid, so I'll get this over quick. I looked up in the CAP website and found 10 references to gum disease, but nothing about chewing gum in the lab. If you put a piece of gum in your mouth BEFORE entering the lab, can you still chew the gum? The reason why I ask is that we are getting a new medical director and we have already have problems. I could not chew gum, then after lunch of beef fajitas with peppers and onions, beans, and salsa, causing me to have dragon breath, I guess I could have a close up discussion with her. Forget the brushing the teeth road. I brush my teeth once a week whether I need to or not (only kidding). JTT From rschoon <@t> email.unc.edu Thu Apr 3 06:21:10 2008 From: rschoon <@t> email.unc.edu (rschoon@email.unc.edu) Date: Thu Apr 3 06:22:08 2008 Subject: [Histonet] F4/80 In-Reply-To: References: Message-ID: <20080403072110.du5icluqqs4c88sc@webmail3.isis.unc.edu> F4/80 is a RAT specific marker for activated macrophages so I am not to surprised that nothing is staining in the mouse tissues. I've used this antibody with great success on rat in the past. Suggest that you search the web maybe stating here: Activated microglia in cortex of mouse models of mucopolysaccharidoses I and IIIB Kazuhiro Ohmi*,, David S. Greenberg*,, Kavitha S. Rajavel*, Sergey Ryazantsev*, Hong Hua Li*, and Elizabeth F. Neufeld*,,?,? * Department of Biological Chemistry and Brain Research Institute, David Geffen School of Medicine, and ? Molecular Biology Institute, University of California, Los Angeles, CA 90095 Contributed by Elizabeth F. Neufeld, December 20, 2002 -N-Acetylglucosaminidase deficiency (mucopolysaccharidosis IIIB, MPS IIIB) and -L-iduronidase deficiency (MPS I) are heritable lysosomal storage diseases; neurodegeneration is prominent in MPS IIIB and in severe cases of MPS I. We have obtained morphologic and molecular evidence for the involvement of microglia in brain pathology of mouse models of the two diseases. In the cortex, a subset of microglia (sometimes perineuronal) consists of cells that are probably phagocytic; they have large storage vacuoles, react with MOMA-2 (monoclonal antibody against macrophages) and Griffonia simplicifolia isolectin IB4, and stain intensely for the lysosomal proteins Lamp-1, Lamp-2, and cathepsin D as well as for GM3 ganglioside. MOMA-2-positive cells appear at 1 and 6 months in MPS IIIB and MPS I mice, respectively, but though their number increases with age, they remain sparse. However, a profusion of cells carrying the macrophage CD68/macrosialin antigen appear in the cortex of both mouse models at 1 month. mRNA encoding CD68/macrosialin also increases at that time, as shown by microarray and Northern blot analyses. Ten other transcripts elevated in both mouse models are associated with macrophage functions, including complement C4, the three subunits of complement C1q, lysozyme M, cathepsins S and Z, cytochrome b558 small subunit, macrophage-specific protein 1, and DAP12. An increase in IFN- and IFN- receptor was observed by immunohistochemistry. These functional increases may represent activation of resident microglia, an influx and activation of blood monocytes, or both. They show an inflammatory component of brain disease in the two MPS, as is known for many neurodegenerative disorders. Robert Schoonhoven Scientist, Pathology MPI Research -------------------------------------------------------------------------------- K.O. and D.S.G. contributed equally to this work. "Dear colleagues, is anybody there who has experiences with a F4/80 antibody? It is supposed to label "activated" microglia. Unfortunatly I don't get a specific staining in mice. Does anyone have an idea? Thanks a lot, F. Neff" From Barry.R.Rittman <@t> uth.tmc.edu Thu Apr 3 06:29:31 2008 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Thu Apr 3 06:29:39 2008 Subject: [Histonet] chewing gum References: <000801c8957c$468f5120$0302a8c0@yourxhtr8hvc4p> Message-ID: Joe Chewing gum can be justified on a medical basis. It was recently shown that chewing gum after meals decreases reflux. A few minutes after starting chewing you are not actually eating, just maintaining salivary flow (unles you actually swallow the gum). You could also ask that what would she do if you had a pebble in your mouth to accomplish the same effect? Two conditions, do not spit in the lab and only use the gum for repairing equipment in case of emergency. Barry ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Joe Nocito Sent: Thu 4/3/2008 6:17 AM To: histonet Subject: [Histonet] chewing gum I know I'm going to feel stupid, so I'll get this over quick. I looked up in the CAP website and found 10 references to gum disease, but nothing about chewing gum in the lab. If you put a piece of gum in your mouth BEFORE entering the lab, can you still chew the gum? The reason why I ask is that we are getting a new medical director and we have already have problems. I could not chew gum, then after lunch of beef fajitas with peppers and onions, beans, and salsa, causing me to have dragon breath, I guess I could have a close up discussion with her. Forget the brushing the teeth road. I brush my teeth once a week whether I need to or not (only kidding). JTT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Thu Apr 3 06:46:13 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Thu Apr 3 06:46:33 2008 Subject: [Histonet] chewing gum In-Reply-To: References: <000801c8957c$468f5120$0302a8c0@yourxhtr8hvc4p> Message-ID: <34BB307EFC9A65429BBB49E330675F720732EEA3@LTA3VS003.ees.hhs.gov> Gee Barry....gum for equipment repairs? Any self-respecting laboratorian knows that you use duct tape for that! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rittman, Barry R Sent: Thursday, April 03, 2008 7:30 AM To: Joe Nocito; histonet Subject: RE: [Histonet] chewing gum Joe Chewing gum can be justified on a medical basis. It was recently shown that chewing gum after meals decreases reflux. A few minutes after starting chewing you are not actually eating, just maintaining salivary flow (unles you actually swallow the gum). You could also ask that what would she do if you had a pebble in your mouth to accomplish the same effect? Two conditions, do not spit in the lab and only use the gum for repairing equipment in case of emergency. Barry ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Joe Nocito Sent: Thu 4/3/2008 6:17 AM To: histonet Subject: [Histonet] chewing gum I know I'm going to feel stupid, so I'll get this over quick. I looked up in the CAP website and found 10 references to gum disease, but nothing about chewing gum in the lab. If you put a piece of gum in your mouth BEFORE entering the lab, can you still chew the gum? The reason why I ask is that we are getting a new medical director and we have already have problems. I could not chew gum, then after lunch of beef fajitas with peppers and onions, beans, and salsa, causing me to have dragon breath, I guess I could have a close up discussion with her. Forget the brushing the teeth road. I brush my teeth once a week whether I need to or not (only kidding). JTT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Thu Apr 3 08:07:01 2008 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Thu Apr 3 07:08:19 2008 Subject: SPAM-LOW: [Histonet] chewing gum In-Reply-To: <000801c8957c$468f5120$0302a8c0@yourxhtr8hvc4p> Message-ID: <1004894925-300717319@pathology.swmed.edu> Back in the day (when I was a kid) I used to get those wax lips that were chewable like gum. I wonder if there is a warning on the para-plast bags about chewing it. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Thursday, April 03, 2008 6:17 AM To: histonet Subject: SPAM-LOW: [Histonet] chewing gum I know I'm going to feel stupid, so I'll get this over quick. I looked up in the CAP website and found 10 references to gum disease, but nothing about chewing gum in the lab. If you put a piece of gum in your mouth BEFORE entering the lab, can you still chew the gum? The reason why I ask is that we are getting a new medical director and we have already have problems. I could not chew gum, then after lunch of beef fajitas with peppers and onions, beans, and salsa, causing me to have dragon breath, I guess I could have a close up discussion with her. Forget the brushing the teeth road. I brush my teeth once a week whether I need to or not (only kidding). JTT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Thu Apr 3 07:17:34 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Thu Apr 3 07:17:53 2008 Subject: SPAM-LOW: [Histonet] chewing gum In-Reply-To: <1004894925-300717319@pathology.swmed.edu> References: <000801c8957c$468f5120$0302a8c0@yourxhtr8hvc4p> <1004894925-300717319@pathology.swmed.edu> Message-ID: <34BB307EFC9A65429BBB49E330675F720732EEA7@LTA3VS003.ees.hhs.gov> Just make sure you aren't using "Plus"..the DMSO might be an issue, LOL! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Thursday, April 03, 2008 9:07 AM To: 'Joe Nocito'; 'histonet' Subject: RE: SPAM-LOW: [Histonet] chewing gum Back in the day (when I was a kid) I used to get those wax lips that were chewable like gum. I wonder if there is a warning on the para-plast bags about chewing it. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Thursday, April 03, 2008 6:17 AM To: histonet Subject: SPAM-LOW: [Histonet] chewing gum I know I'm going to feel stupid, so I'll get this over quick. I looked up in the CAP website and found 10 references to gum disease, but nothing about chewing gum in the lab. If you put a piece of gum in your mouth BEFORE entering the lab, can you still chew the gum? The reason why I ask is that we are getting a new medical director and we have already have problems. I could not chew gum, then after lunch of beef fajitas with peppers and onions, beans, and salsa, causing me to have dragon breath, I guess I could have a close up discussion with her. Forget the brushing the teeth road. I brush my teeth once a week whether I need to or not (only kidding). JTT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From koellingr <@t> comcast.net Thu Apr 3 07:46:03 2008 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Thu Apr 3 07:46:22 2008 Subject: [Histonet] F4/80 Message-ID: <040320081246.24436.47F4D18B00084DBC00005F7422007343649D09020704040A0105@comcast.net> I'm confused. The F4/80 antibody I know (clone BM8) is a rat monoclonal that stains macrophages and macrophage derived lineages in mice beautifully. Kuppfer cells, in spleen, skin, lymph nodes using an anti-rat secondary that has been pre-absorbed against mouse. For peroxidase or immunoflouresence and people all over research use F4/80 on mouse tissues. I think the problem is with what and how are your microglia "activated". Not ALL macrophage markers work on microglia in different states because they are not the exact flavor of a card carrying macrophage. Try a liver or LN and see if you get no staining. Ray Koelling PhenoPath Labs Seattle, WA -------------- Original message -------------- From: rschoon@email.unc.edu > F4/80 is a RAT specific marker for activated macrophages so I am not to > surprised that nothing is staining in the mouse tissues. I've used > this antibody with great success on rat in the past. Suggest that you > search the web maybe stating here: > > Activated microglia in cortex of mouse models of mucopolysaccharidoses > I and IIIB Kazuhiro Ohmi*,, David S. Greenberg*,, Kavitha S. Rajavel*, > Sergey Ryazantsev*, Hong Hua Li*, and Elizabeth F. Neufeld*,,§,¶ * > Department of Biological Chemistry and Brain Research Institute, David > Geffen School of Medicine, and § Molecular Biology Institute, > University of California, Los Angeles, CA 90095 > > Contributed by Elizabeth F. Neufeld, December 20, 2002 > > -N-Acetylglucosaminidase deficiency (mucopolysaccharidosis IIIB, MPS > IIIB) and -L-iduronidase deficiency (MPS I) are heritable lysosomal > storage diseases; neurodegeneration is prominent in MPS IIIB and in > severe cases of MPS I. We have obtained morphologic and molecular > evidence for the involvement of microglia in brain pathology of mouse > models of the two diseases. In the cortex, a subset of microglia > (sometimes perineuronal) consists of cells that are probably > phagocytic; they have large storage vacuoles, react with MOMA-2 > (monoclonal antibody against macrophages) and Griffonia simplicifolia > isolectin IB4, and stain intensely for the lysosomal proteins Lamp-1, > Lamp-2, and cathepsin D as well as for GM3 ganglioside. MOMA-2-positive > cells appear at 1 and 6 months in MPS IIIB and MPS I mice, > respectively, but though their number increases with age, they remain > sparse. However, a profusion of cells carrying the macrophage > CD68/macrosialin antigen appear in the cortex of both mouse models at 1 > month. mRNA encoding CD68/macrosialin also increases at that time, as > shown by microarray and Northern blot analyses. Ten other transcripts > elevated in both mouse models are associated with macrophage functions, > including complement C4, the three subunits of complement C1q, lysozyme > M, cathepsins S and Z, cytochrome b558 small subunit, > macrophage-specific protein 1, and DAP12. An increase in IFN- and IFN- > receptor was observed by immunohistochemistry. These functional > increases may represent activation of resident microglia, an influx and > activation of blood monocytes, or both. They show an inflammatory > component of brain disease in the two MPS, as is known for many > neurodegenerative disorders. > > Robert Schoonhoven > Scientist, Pathology > MPI Research > -------------------------------------------------------------------------------- > K.O. and D.S.G. contributed equally to this work. > > "Dear colleagues, > is anybody there who has experiences with a F4/80 antibody? It is > supposed to label "activated" microglia. Unfortunatly I don't get a > specific staining in mice. > Does anyone have an idea? > Thanks a lot, > > F. Neff" > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Apr 3 08:11:28 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Apr 3 08:11:32 2008 Subject: AW: [Histonet] sections rolling - need ribbon! In-Reply-To: <000501c8955b$8e9f3460$eeeea8c0@dielangs.at> Message-ID: <217664.81678.qm@web65701.mail.ac4.yahoo.com> Hi Gudrun: It all depends on the section thickness. If the softer paraffin is set to section thin it will ribbon better than if it is set to section thick, where ribbons will be more difficult to form. A hard (high melting point) paraffin will always produce better ribbons at any thickness since it holds the tissue better (when cooled). The ribbon formation has also to do with the speed you section, a constant, low speed, rhythmical, produces better ribbons (with any type of paraffin), than sectioning with speed "bursts". That constant low speed motion permits the sections to get in contact better and assures the ribbon better. For rotary microtomes it is all in the wrist! The sliding microtome requires more force, although you can control better the blade movement (over the static specimen) that is an inverse situation with the rotary (where the specimen is moving against a fixed blade). These two opposite configurations, from the mechanical point of view, also influence the ribbon formation. So there is always a trade off between instruments and techniques. Finally, I have never noticed any "mess in your brain". Ren? J. Gudrun Lang wrote: --------------------------------- You rock. That's why Blockbuster's offering you one month of Blockbuster Total Access, No Cost. From mcauliff <@t> umdnj.edu Thu Apr 3 08:33:43 2008 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Apr 3 08:33:37 2008 Subject: [Histonet] F4/80 In-Reply-To: <1207214098.47f4a0122c09a@webmail.med.uni-marburg.de> References: <1207214098.47f4a0122c09a@webmail.med.uni-marburg.de> Message-ID: <47F4DCB7.3080506@umdnj.edu> Greetings! F4/80 does work in mouse brain, staining both "resting" and "activated" microglia". In my hands it is somewhat easier to use than Mac-1 since the rxn.survives fixation better. I would look at your detection system, fresh peroxide, fresh DAB, detection kit mixed properly, etc. Process some liver in parallel as a control. Geoff Dr. med. Frauke Neff wrote: > Dear colleagues, > is anybody there who has experiences with a F4/80 antibody? It is supposed to > label "activated" microglia. Unfortunatly I don't get a specific staining in > mice. > Does anyone have an idea? > Thanks a lot, > > F. Neff > > > > > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From TJJ <@t> Stowers-Institute.org Thu Apr 3 08:33:42 2008 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Thu Apr 3 08:34:16 2008 Subject: [Histonet] Re: F4/80 Message-ID: Dr. Neff, I ran across an ad from Serotec on an F4/80 antibody that works in mouse. It's a rat anti-mouse monoclonal that is reported to work in frozen and paraffin embedded tissues. Here's the link to their datasheet: http://www.ab-direct.com/catalog/datasheet.aspx?ProductCode=MCA497GA&SearchType=RelatedProductsFamily&SearchString=MCA497A488 Best of luck to you! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From anh2006 <@t> med.cornell.edu Thu Apr 3 09:05:20 2008 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Thu Apr 3 09:05:25 2008 Subject: [Histonet] F4/80 In-Reply-To: <47F4DCB7.3080506@umdnj.edu> References: <1207214098.47f4a0122c09a@webmail.med.uni-marburg.de> <47F4DCB7.3080506@umdnj.edu> Message-ID: F4/80 from Serotec works beautifully. Is this the Ab you are using?? > > >Dr. med. Frauke Neff wrote: >>Dear colleagues, >>is anybody there who has experiences with a F4/80 antibody? It is supposed to >>label "activated" microglia. Unfortunatly I don't get a specific staining in >>mice. >>Does anyone have an idea? >>Thanks a lot, >> >>F. Neff >> >> -- From elizabeth.heimrich <@t> bms.com Thu Apr 3 09:05:25 2008 From: elizabeth.heimrich <@t> bms.com (Elizabeth M Heimrich) Date: Thu Apr 3 09:05:45 2008 Subject: [Histonet] F4/80 In-Reply-To: <47F4DCB7.3080506@umdnj.edu> References: <1207214098.47f4a0122c09a@webmail.med.uni-marburg.de> <47F4DCB7.3080506@umdnj.edu> Message-ID: <47F4E425.10708@bms.com> You also need to be certain of the digestion technique you are using... Serotec mouse anti rat F4/80 stains the microglia only if the enzyme digestion is used. ( In my hands anyway!) Watch your fixation time, as well. Good luck, Beth Geoff McAuliffe wrote: > Greetings! > > F4/80 does work in mouse brain, staining both "resting" and > "activated" microglia". In my hands it is somewhat easier to use than > Mac-1 since the rxn.survives fixation better. I would look at your > detection system, fresh peroxide, fresh DAB, detection kit mixed > properly, etc. Process some liver in parallel as a control. > > Geoff > > Dr. med. Frauke Neff wrote: > >> Dear colleagues, >> is anybody there who has experiences with a F4/80 antibody? It is >> supposed to >> label "activated" microglia. Unfortunatly I don't get a specific >> staining in >> mice. >> Does anyone have an idea? >> Thanks a lot, >> >> F. Neff >> >> >> >> >> >> >> >> > > > From dmccaig <@t> ckha.on.ca Thu Apr 3 09:21:36 2008 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Thu Apr 3 09:21:48 2008 Subject: [Histonet] Alcian Blue-Metanil Yellow Message-ID: Is anyone using this stain. We were doing this stain routinely on all esophagus biopsies. Originally it was done manually then programmed on an autostainer. The stain worked great but recently we have been experiencing problems with uneven, inconsistent staining. Has anyone else had problems with this stain? Diana From dusko.trajkovic <@t> pfizer.com Thu Apr 3 09:22:12 2008 From: dusko.trajkovic <@t> pfizer.com (Trajkovic, Dusko) Date: Thu Apr 3 09:22:28 2008 Subject: [Histonet] F4/80 In-Reply-To: <1207214098.47f4a0122c09a@webmail.med.uni-marburg.de> Message-ID: <3AD0BD3142459B4E9B12CBEAFF2B89B206D04862@lajamrexm01.amer.pfizer.com> e-Biosciences has a biotinylated F4/80 antibody that works on mouse tissue. If you need more info, please contact me. Thanks Dusko -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dr. med. Frauke Neff Sent: Thursday, April 03, 2008 2:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] F4/80 Dear colleagues, is anybody there who has experiences with a F4/80 antibody? It is supposed to label "activated" microglia. Unfortunatly I don't get a specific staining in mice. Does anyone have an idea? Thanks a lot, F. Neff ---------------------------------------------------------------- This message was sent using IMP, the Internet Messaging Program. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mwich <@t> 7thwavelabs.com Thu Apr 3 09:36:42 2008 From: mwich <@t> 7thwavelabs.com (Michele Wich) Date: Thu Apr 3 09:36:52 2008 Subject: [Histonet] CAP guidelines for immuno controls Message-ID: <2264717ADC396742A0FF0AAB674F9A0D486C28@7THWAVE-SERVER.7thwave.local> Does anyone know what is required by CAP for keeping IHC controls? Is it necessary to keep a physical log of dates and batches each control is run with? Or is it enough to keep an example of the stained control in the appropriate control box? I've never been clear on this. Any info is greatly appreciated! This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer From bwhitaker <@t> brownpathology.com Thu Apr 3 09:02:30 2008 From: bwhitaker <@t> brownpathology.com (Bonnie Whitaker) Date: Thu Apr 3 09:58:00 2008 Subject: [Histonet] Re: Illegal spam Message-ID: <4D142A11FD20784C9BEA1EA7F014D44C274C@BA1.brownpathology.local> A good thesaurus is very helpful, too ;) Bonnie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Wednesday, April 02, 2008 7:34 PM To: shive003@umn.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Illegal spam Hi, I also have a University email that has very tight security. I do not use that for the Histonet as I subscribe from home. My University email account is also deluged with spam. I do not think the Histonet really has anything to do with spam messages. The best advise I can offer is to filter messages based on the subject line rather than the sender. Filtering messages with "Viagara", "V1agara", "Cialis" and "Cia1is" will be much more helpful. Amos Message: 17 Date: Wed, 2 Apr 2008 09:18:08 -0500 From: "Jan Shivers" Subject: Fw: [Histonet] Re: Illegal spam To: "histonet" Message-ID: <004901c894cc$6030cfb0$b0065486@auxs.umn.edu> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Ever since I subscribed to the Histonet, I've been receiving a lot of spam every day (mostly from people in Africa/Europe, either telling me I've won the lottery or asking me for my bank account number so that they can smuggle money out of their country). I can understand receiving this spam if this was a private home email account, with medium security levels, but I work at a University that has a very tight security system on their incoming email, so the only thing I can figure out is that this mail is getting through via a Histonet member's addressbook. I must have to delete 10-15 of these spams a day (it's the only spam I get, too). Very frustrating. Anybody have any suggestions as to how to eliminate these pesky creatures? Blocking the Sender doesn't work; they just change their addresses. Jan Shivers _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Susan.Pemberton <@t> bmcjax.com Thu Apr 3 10:26:18 2008 From: Susan.Pemberton <@t> bmcjax.com (Pemberton, Susan) Date: Thu Apr 3 10:27:03 2008 Subject: [Histonet] Cassette Labeling Message-ID: <4E59E1E7B5A80F448F47424C8A2FDEDB022955AB@BHEXCHVS02.BH.LOCAL> Gary, Could you give me an item number or other more detailed description for the Hacker storage bins? Thank you, Susan Susan L. Pemberton MS, MT(ASCP)SM,DLM Laboratory Administrative Director . Baptist Health 800 Prudential Drive . Jacksonville, FL 32207 (904) 202-2016 . Fax: (904) 202-2795 ----------------------------------------- NOTICE: This message is confidential, intended for the named recipient(s) and may contain information that is (i) proprietary to the sender, and/or,(ii) privileged, confidential and/or otherwise exempt from disclosure under applicable Florida and federal law, including, but not limited to, privacy standards imposed pursuant to the federal Health insurance Portability and Accountability Act of 1996 ("HIPAA"). Receipt by anyone other than the named recipient(s) is not a waiver of any applicable privilege. Thank you in advance for your compliance with this notice. From nefff <@t> staff.uni-marburg.de Thu Apr 3 11:10:35 2008 From: nefff <@t> staff.uni-marburg.de (Dr. med. Frauke Neff) Date: Thu Apr 3 11:10:44 2008 Subject: [Histonet] F4/80 thankU! In-Reply-To: <1207214098.47f4a0122c09a@webmail.med.uni-marburg.de> References: <1207214098.47f4a0122c09a@webmail.med.uni-marburg.de> Message-ID: <1207239035.47f5017baee4a@webmail.med.uni-marburg.de> Hi colleages, I just wanted to say "thank you" for your good advices. It seems that I use the wrong antibody (from abcam), so I am going to try the serotec one and use liver or lymph nodes as control;-) Cheers, Frauke Quoting "Dr. med. Frauke Neff" : > Dear colleagues, > is anybody there who has experiences with a F4/80 antibody? It is supposed to > label "activated" microglia. Unfortunatly I don't get a specific staining in > mice. > Does anyone have an idea? > Thanks a lot, > > F. Neff > > > > > > > ---------------------------------------------------------------- > This message was sent using IMP, the Internet Messaging Program. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ---------------------------------------------------------------- This message was sent using IMP, the Internet Messaging Program. From bdelescavage <@t> cellnetix.com Thu Apr 3 11:22:34 2008 From: bdelescavage <@t> cellnetix.com (Beth Delescavage) Date: Thu Apr 3 11:26:46 2008 Subject: [Histonet] chewing gum In-Reply-To: <34BB307EFC9A65429BBB49E330675F720732EEA3@LTA3VS003.ees.hhs.gov> Message-ID: You should place the gum down first and then use the duct tape to keep it in place. You can never be too careful! Beth Delescavage, BS, HTL (ASCP) QIHC -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, April 03, 2008 4:46 AM To: Rittman, Barry R; Joe Nocito; histonet Subject: RE: [Histonet] chewing gum Gee Barry....gum for equipment repairs? Any self-respecting laboratorian knows that you use duct tape for that! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rittman, Barry R Sent: Thursday, April 03, 2008 7:30 AM To: Joe Nocito; histonet Subject: RE: [Histonet] chewing gum Joe Chewing gum can be justified on a medical basis. It was recently shown that chewing gum after meals decreases reflux. A few minutes after starting chewing you are not actually eating, just maintaining salivary flow (unles you actually swallow the gum). You could also ask that what would she do if you had a pebble in your mouth to accomplish the same effect? Two conditions, do not spit in the lab and only use the gum for repairing equipment in case of emergency. Barry ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Joe Nocito Sent: Thu 4/3/2008 6:17 AM To: histonet Subject: [Histonet] chewing gum I know I'm going to feel stupid, so I'll get this over quick. I looked up in the CAP website and found 10 references to gum disease, but nothing about chewing gum in the lab. If you put a piece of gum in your mouth BEFORE entering the lab, can you still chew the gum? The reason why I ask is that we are getting a new medical director and we have already have problems. I could not chew gum, then after lunch of beef fajitas with peppers and onions, beans, and salsa, causing me to have dragon breath, I guess I could have a close up discussion with her. Forget the brushing the teeth road. I brush my teeth once a week whether I need to or not (only kidding). JTT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From Charlene.Henry <@t> STJUDE.ORG Thu Apr 3 12:16:24 2008 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Thu Apr 3 12:16:35 2008 Subject: [Histonet] CAP guidelines for immuno controls. . In-Reply-To: <2264717ADC396742A0FF0AAB674F9A0D486C28@7THWAVE-SERVER.7thwave.local> Message-ID: <03E1F5968F60C5448635D49D38B283ED4D8B28E7@SJMEMXMBS11.stjude.sjcrh.local> You must be able to show documentation that all batch controls (this includes negative controls also) have been reviewed by laboratory director or designee and this documentation and control slides must be retained for 2 years. Remember also that if a supervisor/designee is designated by the laboratory director to review IHC controls, that this must be written in a policy. Hope this helps. Charlene -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michele Wich Sent: Thursday, April 03, 2008 9:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP guidelines for immuno controls. . Does anyone know what is required by CAP for keeping IHC controls? Is it necessary to keep a physical log of dates and batches each control is run with? Or is it enough to keep an example of the stained control in the appropriate control box? I've never been clear on this. Any info is greatly appreciated! This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kayd <@t> cytolabpathology.com Thu Apr 3 12:20:36 2008 From: kayd <@t> cytolabpathology.com (Kay Duffy) Date: Thu Apr 3 12:20:27 2008 Subject: [Histonet] (no subject) Message-ID: <147174A7C9FDD344822BD4D944B5589E2E3F5B@08CPSMX.cytolabpathology.com> Unsubscribe From kayd <@t> cytolabpathology.com Thu Apr 3 12:21:04 2008 From: kayd <@t> cytolabpathology.com (Kay Duffy) Date: Thu Apr 3 12:20:57 2008 Subject: [Histonet] unsubscribe Message-ID: <147174A7C9FDD344822BD4D944B5589E2E3F5C@08CPSMX.cytolabpathology.com> From algranth <@t> u.arizona.edu Thu Apr 3 12:40:03 2008 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Thu Apr 3 12:48:32 2008 Subject: [Histonet] Multi spot slides Message-ID: <6.2.3.4.1.20080403103703.01ef4f20@algranth.inbox.email.arizona.edu> I need a quick answer on this- besides Erie, does anyone know of a vendor who sells the "multi spot" slides that can be used for IHC, etc? Thanks!!! Andi Grantham ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From asmith <@t> mail.barry.edu Thu Apr 3 13:35:24 2008 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Thu Apr 3 13:35:34 2008 Subject: [Histonet] RE: illegal spam Message-ID: My e-mail program shows the first line of each message. The first act of my lunch hour is go through my e-mail with my index finger on the delete key. All 40 pieces of spam are deleted in 3 minutes. Then I can look over my colleagues staining problems. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Podiatric Medicine Miami Shores, Florida From MMcCOY <@t> lakelandregional.org Thu Apr 3 13:44:06 2008 From: MMcCOY <@t> lakelandregional.org (Mary McCoy) Date: Thu Apr 3 13:41:57 2008 Subject: [Histonet] PIN-4 Message-ID: <20080403T144406Z_C27C00150000@lakelandregional.org> We have recently started doing PIN-4 triple stain. How do folks charge for double/triple staining on the same slide? Can you charge for each antigen? Mary McCoy HTL(ASCP) Supervisor of Pathology Services Lakeland Regional Health System St. Joseph MI 49098 (269) 982-4891 mmccoy@lakelandregional.org From jconnelly <@t> sleh.com Thu Apr 3 13:57:30 2008 From: jconnelly <@t> sleh.com (Connelly, John) Date: Thu Apr 3 13:57:42 2008 Subject: [Histonet] Control tissue for immunofluorescence Message-ID: <53C8858F28EF504C9B481F0636CE404EB8D42892F3@SLEHEXCH01.sleh.com> We use immunofluorescence for native kidneys. I was wondering what tissue, the participants use for a positive control and how do they store it? Is the tissue cut and place on blank sides for use ahead of time? We routinely run Iga, IgG, IgM, C3, Kappa, Lambda, C1q, albumin and fibrinogen. Thanks for you help, John Connelly, M.D. +++++CONFIDENTIALITY NOTICE+++++ The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. From sbreeden <@t> nmda.nmsu.edu Thu Apr 3 14:00:21 2008 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Apr 3 14:00:25 2008 Subject: [Histonet] CAP Question Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E6259@nmdamailsvr.nmda.ad.nmsu.edu> Can anyone tell me if there are CAP or JCAHO requirements for staffing in an accredited histology lab? Does CAP require that a histology laboratory employ certified/registered HTs in order for that lab to be accredited? Thanks in advance. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From Teri.Hallada <@t> midmichigan.org Thu Apr 3 14:12:18 2008 From: Teri.Hallada <@t> midmichigan.org (Teri.Hallada@midmichigan.org) Date: Thu Apr 3 14:12:31 2008 Subject: [Histonet] CAP Question In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B017E6259@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <8839B08E3ED7364E8CBBD53882C984D50994CC50@MAILSRV01.midmichigan.net> I would also like to add to Sally's question. Does CAP consider immuno's as "high complexity testing"? The Her2 guidelines specifically state that Her2 testing is "high complexity". Does that mean the reading of the Her2 or the performance of the test? If it is the performance of the test, where do other immuno's fit in the scheme? Teresa Hallada BS, MT/CT (ASCP) Lead Cytotechnologist/Histology MidMichigan Health - Gratiot teri.hallada@midmichigan.org 989.463.1101 ext 3423 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, April 03, 2008 3:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP Question Can anyone tell me if there are CAP or JCAHO requirements for staffing in an accredited histology lab? Does CAP require that a histology laboratory employ certified/registered HTs in order for that lab to be accredited? Thanks in advance. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law. The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited. If you have received this email in error, please advise by immediate reply. From renafail <@t> bellsouth.net Thu Apr 3 14:12:13 2008 From: renafail <@t> bellsouth.net (Rena Fail) Date: Thu Apr 3 14:12:33 2008 Subject: [Histonet] PIN-4 In-Reply-To: <20080403T144406Z_C27C00150000@lakelandregional.org> Message-ID: <000001c895be$a06a3920$0301a8c0@RENAD4YK9B8ABE> Yes, the pathologist reports the results of each of the 3 antigens and pin4 can be listed as 3 separate tests. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mary McCoy Sent: Thursday, April 03, 2008 1:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PIN-4 We have recently started doing PIN-4 triple stain. How do folks charge for double/triple staining on the same slide? Can you charge for each antigen? Mary McCoy HTL(ASCP) Supervisor of Pathology Services Lakeland Regional Health System St. Joseph MI 49098 (269) 982-4891 mmccoy@lakelandregional.org From NSEARCY <@t> swmail.sw.org Thu Apr 3 14:38:21 2008 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Thu Apr 3 14:38:37 2008 Subject: [Histonet] Question Message-ID: What are you cytology folks using for IC9D code for reactive dx? Thanks From JWEEMS <@t> sjha.org Thu Apr 3 14:52:50 2008 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Thu Apr 3 14:53:15 2008 Subject: [Histonet] PIN-4 In-Reply-To: <20080403T144406Z_C27C00150000@lakelandregional.org> References: <20080403T144406Z_C27C00150000@lakelandregional.org> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD320518EC0F@sjhaexc02.sjha.org> Yes, you may. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mary McCoy Sent: Thursday, April 03, 2008 2:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PIN-4 We have recently started doing PIN-4 triple stain. How do folks charge for double/triple staining on the same slide? Can you charge for each antigen? Mary McCoy HTL(ASCP) Supervisor of Pathology Services Lakeland Regional Health System St. Joseph MI 49098 (269) 982-4891 mmccoy@lakelandregional.org Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From williamstewart.pathology <@t> gmail.com Thu Apr 3 15:45:50 2008 From: williamstewart.pathology <@t> gmail.com (William Stewart) Date: Thu Apr 3 15:45:54 2008 Subject: Fwd: [Histonet] CAP guidelines for immuno controls. . In-Reply-To: <81a8916f0804031344w1864be02k13646099db38eb4d@mail.gmail.com> References: <2264717ADC396742A0FF0AAB674F9A0D486C28@7THWAVE-SERVER.7thwave.local> <03E1F5968F60C5448635D49D38B283ED4D8B28E7@SJMEMXMBS11.stjude.sjcrh.local> <81a8916f0804031344w1864be02k13646099db38eb4d@mail.gmail.com> Message-ID: <81a8916f0804031345x3945d41ib57b1b52da9606d4@mail.gmail.com> ---------- Forwarded message ---------- From: William Stewart Date: Apr 3, 2008 4:44 PM Subject: Re: [Histonet] CAP guidelines for immuno controls. . To: Michele Wich , "histonet@lists.utsouthwestern.edu " Per the CAP-- ANP.12500 - The "minimum requirement for surgical pathology, providing that these are less stringent than state and federal regulations, are:...Glass Slides (including control slides) and reports - 10 years" Hope this helps! Bill On 4/3/08, Henry, Charlene wrote: > > You must be able to show documentation that all batch controls (this > includes negative controls also) have been reviewed by laboratory director > or designee and this documentation and control slides must be retained for 2 > years. Remember also that if a supervisor/designee is designated by the > laboratory director to review IHC controls, that this must be written in a > policy. Hope this helps. > > Charlene > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michele Wich > Sent: Thursday, April 03, 2008 9:37 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] CAP guidelines for immuno controls. . > > Does anyone know what is required by CAP for keeping IHC controls? Is it > necessary to keep a physical log of dates and batches each control is run > with? Or is it enough to keep an example of the stained control in the > appropriate control box? I've never been clear on this. > > Any info is greatly appreciated! > > > This communication is intended solely for the use of the addressee and may > contain information that is legally privileged, confidential or exempt from > disclosure. If you are not the intended recipient, please note that any > dissemination, distribution, or copying of this communication is strictly > prohibited. Anyone who receives this message in error should notify the > sender immediately and delete it from his or her computer > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From bwhitaker <@t> brownpathology.com Thu Apr 3 16:16:27 2008 From: bwhitaker <@t> brownpathology.com (Bonnie Whitaker) Date: Thu Apr 3 16:11:50 2008 Subject: [Histonet] PIN-4 Message-ID: <4D142A11FD20784C9BEA1EA7F014D44C276C@BA1.brownpathology.local> Joyce, Let me add just a bit to that: "You can bill separately for each if you can distinguish them from each other" (different color and/or different localization). There are some cocktails that cannot be billed separately because they cannot be differentiated. Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Thursday, April 03, 2008 2:53 PM To: Mary McCoy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PIN-4 Yes, you may. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mary McCoy Sent: Thursday, April 03, 2008 2:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PIN-4 We have recently started doing PIN-4 triple stain. How do folks charge for double/triple staining on the same slide? Can you charge for each antigen? Mary McCoy HTL(ASCP) Supervisor of Pathology Services Lakeland Regional Health System St. Joseph MI 49098 (269) 982-4891 mmccoy@lakelandregional.org Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Thu Apr 3 16:24:15 2008 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Apr 3 16:24:49 2008 Subject: [Histonet] CAP Question In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B017E6259@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B017E6259@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82BCF@EMAIL.archildrens.org> I don't think there is a requirement. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, April 03, 2008 2:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP Question Can anyone tell me if there are CAP or JCAHO requirements for staffing in an accredited histology lab? Does CAP require that a histology laboratory employ certified/registered HTs in order for that lab to be accredited? Thanks in advance. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From rjbuesa <@t> yahoo.com Thu Apr 3 16:27:11 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Apr 3 16:27:16 2008 Subject: [Histonet] CAP Question In-Reply-To: <8839B08E3ED7364E8CBBD53882C984D50994CC50@MAILSRV01.midmichigan.net> Message-ID: <776165.51133.qm@web65705.mail.ac4.yahoo.com> IHC, INDIF, DIF, ISH, FISH tests were always considered as high complewxity tests. Ren? J. Teri.Hallada@midmichigan.org wrote: I would also like to add to Sally's question. Does CAP consider immuno's as "high complexity testing"? The Her2 guidelines specifically state that Her2 testing is "high complexity". Does that mean the reading of the Her2 or the performance of the test? If it is the performance of the test, where do other immuno's fit in the scheme? Teresa Hallada BS, MT/CT (ASCP) Lead Cytotechnologist/Histology MidMichigan Health - Gratiot teri.hallada@midmichigan.org 989.463.1101 ext 3423 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, April 03, 2008 3:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP Question Can anyone tell me if there are CAP or JCAHO requirements for staffing in an accredited histology lab? Does CAP require that a histology laboratory employ certified/registered HTs in order for that lab to be accredited? Thanks in advance. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law. The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited. If you have received this email in error, please advise by immediate reply. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- You rock. That's why Blockbuster's offering you one month of Blockbuster Total Access, No Cost. From HornHV <@t> archildrens.org Thu Apr 3 16:27:10 2008 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Apr 3 16:27:18 2008 Subject: [Histonet] CAP Question In-Reply-To: <8839B08E3ED7364E8CBBD53882C984D50994CC50@MAILSRV01.midmichigan.net> References: <4D14F0FC9316DD41972D5F03C070908B017E6259@nmdamailsvr.nmda.ad.nmsu.edu> <8839B08E3ED7364E8CBBD53882C984D50994CC50@MAILSRV01.midmichigan.net> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82BD0@EMAIL.archildrens.org> It is my understanding that high complexity testing is based on the interpretation or reading of the stains, not the technical performance. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri.Hallada@midmichigan.org Sent: Thursday, April 03, 2008 2:12 PM To: sbreeden@nmda.nmsu.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP Question I would also like to add to Sally's question. Does CAP consider immuno's as "high complexity testing"? The Her2 guidelines specifically state that Her2 testing is "high complexity". Does that mean the reading of the Her2 or the performance of the test? If it is the performance of the test, where do other immuno's fit in the scheme? Teresa Hallada BS, MT/CT (ASCP) Lead Cytotechnologist/Histology MidMichigan Health - Gratiot teri.hallada@midmichigan.org 989.463.1101 ext 3423 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, April 03, 2008 3:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP Question Can anyone tell me if there are CAP or JCAHO requirements for staffing in an accredited histology lab? Does CAP require that a histology laboratory employ certified/registered HTs in order for that lab to be accredited? Thanks in advance. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law. The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited. If you have received this email in error, please advise by immediate reply. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From bdelescavage <@t> cellnetix.com Thu Apr 3 16:43:02 2008 From: bdelescavage <@t> cellnetix.com (Beth Delescavage) Date: Thu Apr 3 16:50:48 2008 Subject: [Histonet] CAP Question In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82BCF@EMAIL.archildrens.org> Message-ID: I think you should document the training of your staff and ideally have all ASCP certified folks on the bench but that is not always the case. Beth Delescavage, BS, HTL (ASCP) QIHC -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, April 03, 2008 2:24 PM To: Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP Question I don't think there is a requirement. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, April 03, 2008 2:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP Question Can anyone tell me if there are CAP or JCAHO requirements for staffing in an accredited histology lab? Does CAP require that a histology laboratory employ certified/registered HTs in order for that lab to be accredited? Thanks in advance. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ****************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From Charlene.Henry <@t> STJUDE.ORG Thu Apr 3 16:51:25 2008 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Thu Apr 3 16:51:28 2008 Subject: [Histonet] CAP Question. . In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B017E6259@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <03E1F5968F60C5448635D49D38B283ED4D8B28EC@SJMEMXMBS11.stjude.sjcrh.local> I agree with Hazel; because I do not know of a CAP question that requires certified HTs in a clinical laboratory. These requirements are usually set by the individual laboratories. However you must show that your employees have been trained and are competent to perform these tests. This is also why the proficiency testing (CAP Surveys) require that they be performed by the same laboratory employees that routinely perform these tests. Also this is why controls must be ran with our cases and then reviewed daily by a pathologist or designee. We do require certification in our lab and I do remember JCAHO asking to see the certifications of my techs during one inspection but I can't say for sure that JCAHO requires certification. Charlene Henry HT (ASCP), QIHC Anatomic Pathology Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, April 03, 2008 2:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP Question. . Can anyone tell me if there are CAP or JCAHO requirements for staffing in an accredited histology lab? Does CAP require that a histology laboratory employ certified/registered HTs in order for that lab to be accredited? Thanks in advance. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Thu Apr 3 20:59:56 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Apr 3 20:59:28 2008 Subject: [Histonet] CAP guidelines for immuno controls References: <2264717ADC396742A0FF0AAB674F9A0D486C28@7THWAVE-SERVER.7thwave.local> Message-ID: <007201c895f7$94f00dc0$0302a8c0@yourxhtr8hvc4p> Michele, what I have done in the past, (and has been put through the ringer with different inspectors) was to have a log book with the date, case #, immuno (s) stained, reaction results and the doctor that gave me the control. I would have a stained slide (s) in the control box until that block was exhausted. I then put that slide in a file and held on to it for 5 years. If I made control blocks from wet tissue ( as in a colon cancer case) I would label the block as to the tissue type (colon cancer) and block number, "colon cancer 15" I would right the case number on the side of the block (s) so the block could be referenced back to the log book. There might be easier ways to do it, but every time an inspector looked at it, they were impressed with our ability to go back to the original case. Hope this helps. JTT ----- Original Message ----- From: "Michele Wich" To: Sent: Thursday, April 03, 2008 9:36 AM Subject: [Histonet] CAP guidelines for immuno controls Does anyone know what is required by CAP for keeping IHC controls? Is it necessary to keep a physical log of dates and batches each control is run with? Or is it enough to keep an example of the stained control in the appropriate control box? I've never been clear on this. Any info is greatly appreciated! This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Thu Apr 3 21:10:10 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Thu Apr 3 21:10:14 2008 Subject: [Histonet] Re: Illegal Spam Message-ID: Jan Shivers notes: >>Ever since I subscribed to the Histonet, I've been receiving a lot of spam every day (mostly from people in Africa/Europe, either telling me I've won the lottery or asking me for my bank account number so that they can smuggle money out of their country).<< I don't think this has anything to do with Histonet. What are called 419 scams (pronounced four-one-nine, apparently named after the relevant section of the Nigerian penal code) apparently originate in Nigeria, where they constitute the country's third biggest source of foreign exchange. Although they're easy for human eyes to recognize, they're artfully designed to slip through spam filters. - Gmail has extremely efficient spam filters - since they electronically read all your e-mails in order o send you relevant advertising) - and almost nothing but 419 scams slips through. Hormel, which makes the canned meat product originally called Spam, has taken a creative approach - every time you go to Gmail's spam box to dump your 419 mail, you get a link to a recipe for Spam. Hormel clearly understands the ancient P.R. adage that there is no such thing as bad publicity. Bob Richmond Spamurai Pathologist Knoxville TN (but born in Hawai'i, which consumes 10 times as much Spam per capita as the other 49) From koellingr <@t> comcast.net Thu Apr 3 23:16:51 2008 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Thu Apr 3 23:16:56 2008 Subject: [Histonet] granules after X-gal staining Message-ID: <040420080416.24249.47F5ABB30006E5C500005EB922134843739D09020704040A0105@comcast.net> I haven't seen any replies and I am scientifically curious. The beta-galactosidase is simply the enzyme that splits x-gal to eventually produce that classical blue chromogen deposit. The acinar cells of pancreas are laden with known ..ase's (lipoxygenase, proteases, amylase, lipase, elastase, tryptase, etc, etc ase's) and probably unidentified ones. Is it possible that some promiscuous enzyme is substituting enzymatically for beta-galactosidase to get your staining of tiny round blue granules in cytoplasm. If you are working in frozens, your enzymes could all be very active. Have looked at lots of x-gal staining but never in pancreas. Have you stained a normal, not b-gal expressing, mouse pancreas? Am curious and hope someone has done this. Ray Koelling PhenoPath Labs Seattle, WA -------------- Original message -------------- From: Yves Heremans > Dear Histonetters, > > Does anyone know why I am getting granules (tiny, round blue granules > in the cytoplasm) after X-gal staining on frozen sections of mouse > pancreas ? > > Regards, > > Yves > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rachelr <@t> nei.nih.gov Fri Apr 4 07:24:30 2008 From: rachelr <@t> nei.nih.gov (Rachel, Rivka (NIH/NEI) [E]) Date: Fri Apr 4 07:24:39 2008 Subject: [Histonet] granules after X-gal staining References: <040420080416.24249.47F5ABB30006E5C500005EB922134843739D09020704040A0105@comcast.net> Message-ID: We used to see this all the time and couldn't figure out the problem. The histologist even tried filtering all the solutions used for perfusion, staining solutions and many other things but never could determine the cause. Any suggestions? -----Original Message----- From: koellingr@comcast.net [mailto:koellingr@comcast.net] Sent: Fri 4/4/2008 12:16 AM To: Yves Heremans; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] granules after X-gal staining I haven't seen any replies and I am scientifically curious. The beta-galactosidase is simply the enzyme that splits x-gal to eventually produce that classical blue chromogen deposit. The acinar cells of pancreas are laden with known ..ase's (lipoxygenase, proteases, amylase, lipase, elastase, tryptase, etc, etc ase's) and probably unidentified ones. Is it possible that some promiscuous enzyme is substituting enzymatically for beta-galactosidase to get your staining of tiny round blue granules in cytoplasm. If you are working in frozens, your enzymes could all be very active. Have looked at lots of x-gal staining but never in pancreas. Have you stained a normal, not b-gal expressing, mouse pancreas? Am curious and hope someone has done this. Ray Koelling PhenoPath Labs Seattle, WA -------------- Original message -------------- From: Yves Heremans > Dear Histonetters, > > Does anyone know why I am getting granules (tiny, round blue granules > in the cytoplasm) after X-gal staining on frozen sections of mouse > pancreas ? > > Regards, > > Yves > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston <@t> nationwidechildrens.org Fri Apr 4 08:07:47 2008 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Fri Apr 4 08:08:25 2008 Subject: [Histonet] granules after X-gal staining In-Reply-To: Message-ID: <979FF5962E234F45B06CF0DB7C1AABB2166F4D7E@chi2k3ms01.columbuschildrens.net> Yves, You don't say whether or not you are briefly fixing the sections. The binding of the b-galactosidase to lysosomes is relatively loose, and that may contribute to what you are seeing. Brief fixation in paraformaldehyde vapor may suffice. Also, something worth considering, have you tried inhibiting the enzyme with e.g. D-galactonolactone, lactose and/or p-chloromercuribenzoate? This is only speculation as I have never come across this phenomenon personally, but, as with Ray, have never performed the technique on pancreas. One way to get by the problem of diffusion artefact, if that is what this is, would be to employ a semi-permeable membrane enzyme histochemical technique. Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 Ronald.Houston@NationwideChildrens.org Columbus Children's Hospital is now Nationwide Children's Hospital www.NationwideChildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rachel, Rivka (NIH/NEI) [E] Sent: Friday, April 04, 2008 8:25 AM To: koellingr@comcast.net; Yves Heremans; histonet@lists.utsouthwestern.edu Cc: Smith, Roberta (SAIC) Subject: RE: [Histonet] granules after X-gal staining We used to see this all the time and couldn't figure out the problem. The histologist even tried filtering all the solutions used for perfusion, staining solutions and many other things but never could determine the cause. Any suggestions? -----Original Message----- From: koellingr@comcast.net [mailto:koellingr@comcast.net] Sent: Fri 4/4/2008 12:16 AM To: Yves Heremans; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] granules after X-gal staining I haven't seen any replies and I am scientifically curious. The beta-galactosidase is simply the enzyme that splits x-gal to eventually produce that classical blue chromogen deposit. The acinar cells of pancreas are laden with known ..ase's (lipoxygenase, proteases, amylase, lipase, elastase, tryptase, etc, etc ase's) and probably unidentified ones. Is it possible that some promiscuous enzyme is substituting enzymatically for beta-galactosidase to get your staining of tiny round blue granules in cytoplasm. If you are working in frozens, your enzymes could all be very active. Have looked at lots of x-gal staining but never in pancreas. Have you stained a normal, not b-gal expressing, mouse pancreas? Am curious and hope someone has done this. Ray Koelling PhenoPath Labs Seattle, WA -------------- Original message -------------- From: Yves Heremans > Dear Histonetters, > > Does anyone know why I am getting granules (tiny, round blue granules > in the cytoplasm) after X-gal staining on frozen sections of mouse > pancreas ? > > Regards, > > Yves > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From ploykasek <@t> phenopath.com Fri Apr 4 10:05:36 2008 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Fri Apr 4 10:05:53 2008 Subject: [Histonet] SATB1 antibody Message-ID: Hi all. TGIF. Quick question. Wondering if anyone is using an antibody to SATB1 on FFPE human tissues. If so, what antibody are you using? We are looking to use this antibody on breast cancer tissues. There are a couple of articles in the literature using this antibody, but unfortunately it is unclear in the articles exactly which antibody they are using. Thanks for the help. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From sbreeden <@t> nmda.nmsu.edu Fri Apr 4 10:28:11 2008 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri Apr 4 10:28:18 2008 Subject: [Histonet] Looking for Ken Groehler Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E625B@nmdamailsvr.nmda.ad.nmsu.edu> I'd like to contact Ken Groehler; he wrote a piece called "Histowoes" some years ago. If someone knows how to contact him, please let me know. Thanks. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From Tracey.Lenek <@t> CLS.ab.ca Fri Apr 4 11:17:59 2008 From: Tracey.Lenek <@t> CLS.ab.ca (Tracey.Lenek@CLS.ab.ca) Date: Fri Apr 4 11:18:25 2008 Subject: [Histonet] Spirochete Controls Message-ID: <695BA3B5A811274182C175122F8663C02957C4@mail1.calgary.com> Hi, Does anyone know of a supplier for FFPE spirochete controls to be used for Warthin-Starry? Thanks in advance. Tracey CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. From we3smitty <@t> yahoo.com Fri Apr 4 12:18:32 2008 From: we3smitty <@t> yahoo.com (angela smith) Date: Fri Apr 4 12:18:39 2008 Subject: [Histonet] does anyone know: Message-ID: <20241.52227.qm@web62014.mail.re1.yahoo.com> Hello, Years ago I worked with Rose Refender and I know she moved to Georgia or South Carolina some time around 1998. If anyone knows her I would love to get back in touch with her. She and I worked together at Evangelical Community Hospital in Lewisburg PA. Thanks, Angela --------------------------------- You rock. That's why Blockbuster's offering you one month of Blockbuster Total Access, No Cost. From trathborne <@t> somerset-healthcare.com Fri Apr 4 12:31:35 2008 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Fri Apr 4 12:31:41 2008 Subject: [Histonet] Spirochete Controls In-Reply-To: <695BA3B5A811274182C175122F8663C02957C4@mail1.calgary.com> Message-ID: Try Sigma Diagnostics @ 800-325-3010. Catalog #S 2645 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Tracey.Lenek@CLS.ab.ca Sent: Friday, April 04, 2008 12:18 PM To: histonet@lists.utsouthwestern.edu Cc: Heather.Heffernan@cls.ab.ca Subject: [Histonet] Spirochete Controls Hi, Does anyone know of a supplier for FFPE spirochete controls to be used for Warthin-Starry? Thanks in advance. Tracey CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. From Janet.Bonner <@t> FLHOSP.ORG Fri Apr 4 13:33:53 2008 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Apr 4 13:36:43 2008 Subject: [Histonet] Spirochete Controls References: <695BA3B5A811274182C175122F8663C02957C4@mail1.calgary.com> Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F25C8@fhosxchmb006.ADVENTISTCORP.NET> Newcomer Supplies has a great selection of controls including on for the spirochetes. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Tracey.Lenek@CLS.ab.ca Sent: Fri 4/4/2008 12:17 PM To: histonet@lists.utsouthwestern.edu Cc: Heather.Heffernan@cls.ab.ca Subject: [Histonet] Spirochete Controls Hi, Does anyone know of a supplier for FFPE spirochete controls to be used for Warthin-Starry? Thanks in advance. Tracey CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From marktarango <@t> gmail.com Fri Apr 4 13:46:12 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Fri Apr 4 13:46:18 2008 Subject: [Histonet] SATB1 antibody In-Reply-To: References: Message-ID: <5b6eb13e0804041146s22ee50bfm8c301877c4050b0d@mail.gmail.com> I've used this antibody looking at T-cells. I used abcam ab49061 (1:50 - 1:100) for two hours with Diva Decloaker (biocare) 125 degrees C in the pressure cooker and polymer detection (mach 4 from biocare). I never stained breast with this protocol... only lymphoid tissue and bone marrow. Anyway, you can see the T-zone in the tonsil light up, and you'll know it's working right. On Fri, Apr 4, 2008 at 8:05 AM, Patti Loykasek wrote: > Hi all. TGIF. Quick question. Wondering if anyone is using an antibody to > SATB1 on FFPE human tissues. If so, what antibody are you using? We are > looking to use this antibody on breast cancer tissues. There are a couple > of > articles in the literature using this antibody, but unfortunately it is > unclear in the articles exactly which antibody they are using. Thanks for > the help. > > > Patti Loykasek BS, HTL, QIHC > PhenoPath Laboratories > Seattle, WA > > > > > This e-mail message, including any attachments, is for the sole use of the > intended recipients and may contain privileged information. Any > unauthorized > review, use, disclosure or distribution is prohibited. If you are not the > intended > recipient, please contact the sender by e-mail and destroy all copies of > the > original message, or you may call PhenoPath Laboratories, Seattle, WA > U.S.A. > at (206) 374-9000. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From garret.t.miyamoto <@t> us.army.mil Fri Apr 4 14:34:27 2008 From: garret.t.miyamoto <@t> us.army.mil (Miyamoto, Garret T Mr CIV USA USAMEDCOM) Date: Fri Apr 4 14:36:32 2008 Subject: [Histonet] Re: Spirochete Controls In-Reply-To: <0JYT002X1BHO0OF0@mail2.us.army.mil> References: <0JYT002X1BHO0OF0@mail2.us.army.mil> Message-ID: ----- Original Message ----- From: histonet-request@lists.utsouthwestern.edu Date: Friday, April 4, 2008 8:03 am Subject: Histonet Digest, Vol 53, Issue 9 To: histonet@lists.utsouthwestern.edu > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. SATB1 antibody (Patti Loykasek) > 2. Looking for Ken Groehler (Breeden, Sara) > 3. Spirochete Controls (Tracey.Lenek@CLS.ab.ca) > > > ------------------------------------------------------------------- > --- > > Message: 1 > Date: Fri, 04 Apr 2008 08:05:36 -0700 > From: Patti Loykasek > Subject: [Histonet] SATB1 antibody > To: histonet > Message-ID: > Content-Type: text/plain; charset="US-ASCII" > > Hi all. TGIF. Quick question. Wondering if anyone is using an > antibody to > SATB1 on FFPE human tissues. If so, what antibody are you using? > We are > looking to use this antibody on breast cancer tissues. There are a > couple of > articles in the literature using this antibody, but unfortunately > it is > unclear in the articles exactly which antibody they are using. > Thanks for > the help. > > > Patti Loykasek BS, HTL, QIHC > PhenoPath Laboratories > Seattle, WA > > > > > This e-mail message, including any attachments, is for the sole > use of the > intended recipients and may contain privileged information. Any > unauthorized > review, use, disclosure or distribution is prohibited. If you are > not the intended > recipient, please contact the sender by e-mail and destroy all > copies of the > original message, or you may call PhenoPath Laboratories, Seattle, > WA U.S.A. > at (206) 374-9000. > > > > > ------------------------------ > > Message: 2 > Date: Fri, 4 Apr 2008 09:28:11 -0600 > From: "Breeden, Sara" > Subject: [Histonet] Looking for Ken Groehler > To: > Message-ID: > <4D14F0FC9316DD41972D5F03C070908B017E625B@nmdamailsvr.nmda.ad.nmsu.edu> > > Content-Type: text/plain; charset="us-ascii" > > I'd like to contact Ken Groehler; he wrote a piece called "Histowoes" > some years ago. If someone knows how to contact him, please let me > know. Thanks. > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > > > ------------------------------ > > Message: 3 > Date: Fri, 4 Apr 2008 10:17:59 -0600 > From: > Subject: [Histonet] Spirochete Controls > To: > Cc: Heather.Heffernan@cls.ab.ca > Message-ID: <695BA3B5A811274182C175122F8663C02957C4@mail1.calgary.com> > Content-Type: text/plain; charset="iso-8859-1" > > Hi, > > Does anyone know of a supplier for FFPE spirochete controls to be > used for Warthin-Starry? > Thanks in advance. > Tracey > > CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may > contain confidential, personal and/or privileged information > intended for a specific purpose and recipient. If you are not the > intended recipient do not disclose, copy, retain, distribute, use > or modify any of the contents of this transmission. If you > received this transmission in error please notify me immediately > by return e-mail or telephone and destroy the entire transmission > and any copies produced. Thank you. > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 53, Issue 9 > *************************************** > Tracey, You can purchase them at Newcomer Supply: phone (800)383-7799, fax (608)831-0866, website www.newcomersupply.com Have a great day! Garret From ROrr <@t> enh.org Fri Apr 4 15:50:42 2008 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Fri Apr 4 15:50:46 2008 Subject: [Histonet] Triple stain charge Message-ID: Hi Mary, I'll send you the correspondence I have (it's a few years old) directly from CAP. It concurs with the other response you rec'd on the subject. You can charge for all 3 as long as the Doc makes comment on each antibody expression. Becky Orr CLA,HT(ASCP)QIHC Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 -----Original Message----- Message: 6 Date: Thu, 3 Apr 2008 14:44:06 -0400 From: "Mary McCoy" Subject: [Histonet] PIN-4 To: histonet@lists.utsouthwestern.edu Message-ID: <20080403T144406Z_C27C00150000@lakelandregional.org> Content-Type: text/plain; charset="utf-8" We have recently started doing PIN-4 triple stain. How do folks charge for double/triple staining on the same slide? Can you charge for each antigen? Mary McCoy HTL(ASCP) Supervisor of Pathology Services Lakeland Regional Health System St. Joseph MI 49098 (269) 982-4891 mmccoy@lakelandregional.org ------------------------------ From LSebree <@t> uwhealth.org Fri Apr 4 16:09:10 2008 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Fri Apr 4 16:10:15 2008 Subject: [Histonet] Looking for Ken Groehler References: <4D14F0FC9316DD41972D5F03C070908B017E625B@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: Sally, Ken's been retired from the VA Hospital here for a number of years. I could look him up in the phone book here if you're still interested in contacting him. Linda ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Breeden, Sara Sent: Fri 4/4/2008 10:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Looking for Ken Groehler I'd like to contact Ken Groehler; he wrote a piece called "Histowoes" some years ago. If someone knows how to contact him, please let me know. Thanks. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Masterson_John <@t> Allergan.com Fri Apr 4 16:40:03 2008 From: Masterson_John <@t> Allergan.com (Masterson_John) Date: Fri Apr 4 16:41:31 2008 Subject: [Histonet] (no subject) In-Reply-To: Message-ID: <0C58C4F16F0B67448318A38041CADE4B01594DAA@IRMAIL133.irvine.allergan.com> Does anyone have a favorite mouse on mouse IHC kit they would recommend? I'm not sure how many of them are out there these days. John From dean1252 <@t> yahoo.com Fri Apr 4 23:27:40 2008 From: dean1252 <@t> yahoo.com (Nadine Abbott) Date: Fri Apr 4 23:27:42 2008 Subject: [Histonet] looking for Eric Dye Message-ID: <169746.72390.qm@web30403.mail.mud.yahoo.com> Am looking for Eric Dye. Was previously a healthcare recruiter for Ategra Systems. It would be much appreciated if anyone who knows his whereabouts would email me. Thanks so much. Dean A. --------------------------------- You rock. That's why Blockbuster's offering you one month of Blockbuster Total Access, No Cost. From rjbuesa <@t> yahoo.com Sat Apr 5 11:41:25 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Apr 5 11:41:30 2008 Subject: [Histonet] Alzheimer stain In-Reply-To: <47F2228F.4347.0054.0@ah.org> Message-ID: <996511.95361.qm@web65705.mail.ac4.yahoo.com> I use the following procedure for Bielschowsky: 1- silver solution (20% aq.) 2- 0.39% aq. formaldehyde (developer) 3- 0.28% aq. ammonium hydroxide (interrupter) 4- 5% aq. sodium thiosulfate (reducing solution). I never used sodium carbonate Ren? J. Behnaz Sohrab wrote: Question regarding Sodium Carbonate Solution in Bielschowsky method for Alzheimer. Can I substitute Carbonate? Or any one has a better method for this stain? ( I do not have Sodium Carbonate, and no time to order it). Thanks,Behnaz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- You rock. That's why Blockbuster's offering you one month of Blockbuster Total Access, No Cost. From Rcartun <@t> harthosp.org Sun Apr 6 08:58:46 2008 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Sun Apr 6 08:59:02 2008 Subject: [Histonet] Triple stain charge In-Reply-To: References: Message-ID: <47F89ED6020000770000BD07@gwmail4.harthosp.org> We have an extremely busy urology service here at Hartford Hospital. I have always found it interesting that we rarely do anything more than a high molecular weight cytokeratin (clone 34BetaE12) for documenting the presence of prostatic adenocarcinoma (when needed). Yes, we will do a P504S occasionally (along with HMW-CK), but that's it. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Orr, Rebecca" 04/04/08 4:50 PM >>> Hi Mary, I'll send you the correspondence I have (it's a few years old) directly from CAP. It concurs with the other response you rec'd on the subject. You can charge for all 3 as long as the Doc makes comment on each antibody expression. Becky Orr CLA,HT(ASCP)QIHC Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 -----Original Message----- Message: 6 Date: Thu, 3 Apr 2008 14:44:06 -0400 From: "Mary McCoy" Subject: [Histonet] PIN-4 To: histonet@lists.utsouthwestern.edu Message-ID: <20080403T144406Z_C27C00150000@lakelandregional.org> Content-Type: text/plain; charset="utf-8" We have recently started doing PIN-4 triple stain. How do folks charge for double/triple staining on the same slide? Can you charge for each antigen? Mary McCoy HTL(ASCP) Supervisor of Pathology Services Lakeland Regional Health System St. Joseph MI 49098 (269) 982-4891 mmccoy@lakelandregional.org ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From mprice26 <@t> juno.com Sun Apr 6 19:10:15 2008 From: mprice26 <@t> juno.com (mprice26@juno.com) Date: Sun Apr 6 19:11:53 2008 Subject: [Histonet] CAP Question Message-ID: <20080406.191015.793.0@webmail08.dca.untd.com> There is no requirement or standard. CLIA also does not regulate Histology personnel. The Pathologist is the technical/medical director and histo techs work under the supervision of the pathologist. Marsha Price _____________________________________________________________ Start Email Marketing - fast, affordable, and measurable. Click here. http://thirdpartyoffers.juno.com/TGL2121/fc/Ioyw6i3l4wZepdak1gB9Burv2Bb6Zxt3vcwGzP94L0RSgGtgd9lNim/ From rydomsal <@t> yahoo.com Sun Apr 6 23:50:36 2008 From: rydomsal <@t> yahoo.com (Ryan Dominique Salazar) Date: Sun Apr 6 23:50:41 2008 Subject: [Histonet] tissue linings Message-ID: <234519.72037.qm@web52306.mail.re2.yahoo.com> Histopeeps, Any suggestions on how to thoroughly fix the tissue linings (intestines, stomach)? We are currently using minutien pins and corkboard immersed in the fixative but it is too troublesome to transfer the tissues prior to shipment. Any consumables that you can recommend?as much as possible, a reliable supplier please. Thanks for your help everyone. Ryan Salazar Maccine Pte Ltd --------------------------------- You rock. That's why Blockbuster's offering you one month of Blockbuster Total Access, No Cost. From rydomsal <@t> yahoo.com Mon Apr 7 01:29:15 2008 From: rydomsal <@t> yahoo.com (Ryan Dominique Salazar) Date: Mon Apr 7 01:29:19 2008 Subject: [Histonet] tissue linings Message-ID: <454362.59268.qm@web52301.mail.re2.yahoo.com> Histopeeps, Any suggestions on how to thoroughly fix the tissue linings (intestines, stomach)? We are currently using minutien pins and corkboard immersed in the fixative but it is too troublesome to transfer the tissues prior to shipment. Any consumables that you can recommend?as much as possible, a reliable supplier please. Thanks for your help everyone. Ryan Salazar Maccine Pte Ltd --------------------------------- You rock. That's why Blockbuster's offering you one month of Blockbuster Total Access, No Cost. From t.pan <@t> imb.uq.edu.au Mon Apr 7 02:13:12 2008 From: t.pan <@t> imb.uq.edu.au (Timothy Pan) Date: Mon Apr 7 02:13:18 2008 Subject: [Histonet] best way to reduce meniscus Message-ID: <5DB2CD52-47F8-42CA-9EF3-F967F44AEE8B@imb.uq.edu.au> Hi all, I'm new this list. I'm just wondering if anyone knew of some good ways of reducing meniscus in 96-well plates. I've so far had these advice: Add some BSA and/or centrifuge @ low G's. Thanks! Regards Tim From Jeannette.Mitchell <@t> vtmednet.org Mon Apr 7 02:31:30 2008 From: Jeannette.Mitchell <@t> vtmednet.org (Mitchell, Jeannette M.) Date: Mon Apr 7 02:34:07 2008 Subject: [Histonet] Region I Histotechnology Symposium Message-ID: The brochure for the Region I Symposium has been put on the following website: http://www.geocities.com/nshregion1/VTandNH.html http://www.geocities.com/nshregion1/Region1news.html Thank you to Jason Burrill for helping out with this Jeannette Mitchell From godsgalnow <@t> aol.com Mon Apr 7 07:29:14 2008 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Mon Apr 7 07:29:19 2008 Subject: [Histonet] industry standards Message-ID: <8CA66C2201A21FA-C78-21B7@Webmail-mg14.sim.aol.com> I know that this has come up before, but I need to refresh my memory.......are there any published industry standards for how long it should take to do any given task in histology from data entry to coverslipping? I did our FTE analysis for my boss to show him for the amount of work we have that we are short 1.5 FTE's...which of course I did by using how long it takes my employees to do the work we have/task (2 minutes to cut a block, etc).? But, he wants to know if this is optimal compared to industry standards. Can anyone help me? Roxanne From MadaryJ <@t> MedImmune.com Mon Apr 7 08:57:55 2008 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Mon Apr 7 08:59:25 2008 Subject: [Histonet] Mereceds Med has WS controls as well, EOM Message-ID: Nick Madary, HT/HTL(ASCP)QIHC Histology Mgr, Medimmune 301.398.6360(lab), 4745(vm),9745(fax) "To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation." To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From rjbuesa <@t> yahoo.com Mon Apr 7 09:29:34 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Apr 7 09:29:39 2008 Subject: [Histonet] industry standards In-Reply-To: <8CA66C2201A21FA-C78-21B7@Webmail-mg14.sim.aol.com> Message-ID: <36685.92448.qm@web65710.mail.ac4.yahoo.com> Hi Roxanne: There are NO industry standards per se, but there are some studies. There is one about benchmarks by Valenstein et al. It is the result of a study commissioned by CAP with data from 165 medical laboratories that include information on histology. Each and every consulting company has some standards that they apply when organizing a laboratory to "comply" with some management method (or fad). I have a study on productivity, and another on staffing that may be helpful and if you want I can send them to you. Ren? J. godsgalnow@aol.com wrote: I know that this has come up before, but I need to refresh my memory.......are there any published industry standards for how long it should take to do any given task in histology from data entry to coverslipping? I did our FTE analysis for my boss to show him for the amount of work we have that we are short 1.5 FTE's...which of course I did by using how long it takes my employees to do the work we have/task (2 minutes to cut a block, etc).? But, he wants to know if this is optimal compared to industry standards. Can anyone help me? Roxanne _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- You rock. That's why Blockbuster's offering you one month of Blockbuster Total Access, No Cost. From crochieresteve <@t> aol.com Mon Apr 7 12:50:16 2008 From: crochieresteve <@t> aol.com (crochieresteve@aol.com) Date: Mon Apr 7 12:50:29 2008 Subject: [Histonet] cjd brain tissue Message-ID: <8CA66EEF8C6BF2E-13BC-E30@WEBMAIL-DC05.sysops.aol.com> Does anyone have any experience processing possible cjd brain tissue. My procedure diicates fixation in formalin followed by treatment in formic acid. Does this acid harm the morphology? Also, have there been any documented cases of a haito tech contracting cjd from a specimen? just wondering on the second question sc From bob.nienhuis <@t> gmail.com Mon Apr 7 12:51:48 2008 From: bob.nienhuis <@t> gmail.com (Bob Nienhuis) Date: Mon Apr 7 12:51:57 2008 Subject: [Histonet] Sliding microtome blade, a-profile Message-ID: <45109da50804071051u7440c3c7xb693bbdcc8c7679b@mail.gmail.com> Can anyone point me to a source for an a-profile blade for an AO 860 sliding microtome? FYI, the a-profile blades had a concave upper surface and a flat lower surface. Generally used for cutting celloidin sections. Bob Nienhuis Neurobiology Research, M/S 151A3 UCLA / VA Medical Center North Hills, CA From tim.morken <@t> thermofisher.com Mon Apr 7 12:53:06 2008 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Mon Apr 7 12:53:44 2008 Subject: [Histonet] Handi-vac for frozen tissue or block storage? Message-ID: <6BFF6D137DF6BC43B33891BA96E83B1901521C25@PGHCR-EXMB-VS-1.na.fshrnet.com> Just wondering...has anyone every tried one of these vacuum-freezerbag devices for storing frozen blocks or tissue? If so, good or bad results? Like: www.handi-vac.com Tim Morken Lab Vision Products Anatomical Pathology ThermoFisher Scientific From jennifer.l.hofecker <@t> Vanderbilt.Edu Mon Apr 7 13:12:28 2008 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Mon Apr 7 13:12:32 2008 Subject: [Histonet] cjd brain tissue In-Reply-To: <8CA66EEF8C6BF2E-13BC-E30@WEBMAIL-DC05.sysops.aol.com> Message-ID: <898D946569A27444B65667A49C0740520175B26F@mailbe06.mc.vanderbilt.edu> Hello. Yes, fixation in formalin followed by full strength formic acid for one hour, is the appropriate method. The secret is to return the sections to formalin (preferably at least overnight) following the formic acid treatment. If you do not, your morphology will suffer. I recommend checking out the CDC website, it is an excellent resource. ( www.cdc.gov )Also, I encourage taking just a few "scout" sections of the suspected CJD brain and doing the formic acid treatment, leaving the remainder in formalin. If those sections rule out CJD, you still have the other tissue in formalin to process routinely. Please don't hesitate to contact me "off-List" if you need more information. This was a VERY BRIEF answer to your question and in no way is intended to be a full fledged protocol -flame resistant suit here I come :) Have a great week. Jennifer L. Hofecker, HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph. (615)343-0083 fax. (615)343-7089 NSH Quality Control Committee Chair -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of crochieresteve@aol.com Sent: Monday, April 07, 2008 12:50 PM To: histonet@pathology.swmed.edu Subject: [Histonet] cjd brain tissue Does anyone have any experience processing possible cjd brain tissue. My procedure diicates fixation in formalin followed by treatment in formic acid. Does this acid harm the morphology? Also, have there been any documented cases of a haito tech contracting cjd from a specimen? just wondering on the second question sc _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JPCOLEMA <@t> sentara.com Mon Apr 7 13:58:45 2008 From: JPCOLEMA <@t> sentara.com (JOHN P COLEMAN) Date: Mon Apr 7 13:59:09 2008 Subject: [Histonet] Surgipath's O-fix for breast fixation. Message-ID: We routinely use Surgipath's O-fix on breast tissue for subsequent Her-2 testing via IHC and FISH. Is this now disallowed? According to the labeling, O-fix is a formalin based fixative with ethanol and acetic acid added. I would consider it an alcohol based fixative and disallowed only in the absence of the formalin. John P.J. Coleman HT(ASCP)QIHC Clinical Specialist Anatomic Pathology Sentara Laboratory Services Cell/Voicemail(757)335-2159 pager: (757)456-6695 From rjbuesa <@t> yahoo.com Mon Apr 7 15:47:00 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Apr 7 15:47:05 2008 Subject: [Histonet] Surgipath's O-fix for breast fixation. Message-ID: <704535.31547.qm@web65706.mail.ac4.yahoo.com> When a protocol is approved, it is so to be used as approved and does not allow deviations, IF you want to avoid any payment problems. It has nothing to do with adequacy, but with fully following the protocol, regardless of any consideration. What is accepted is NBF, nothing else. Ren? J. JOHN P COLEMAN wrote: We routinely use Surgipath's O-fix on breast tissue for subsequent Her-2 testing via IHC and FISH. Is this now disallowed? According to the labeling, O-fix is a formalin based fixative with ethanol and acetic acid added. I would consider it an alcohol based fixative and disallowed only in the absence of the formalin. John P.J. Coleman HT(ASCP)QIHC Clinical Specialist Anatomic Pathology Sentara Laboratory Services Cell/Voicemail(757)335-2159 pager: (757)456-6695 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- You rock. That's why Blockbuster's offering you one month of Blockbuster Total Access, No Cost. From Jackie.O'Connor <@t> abbott.com Mon Apr 7 15:47:39 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Mon Apr 7 15:47:52 2008 Subject: [Histonet] Fishing for GMA info In-Reply-To: <2264717ADC396742A0FF0AAB674F9A0D486C24@7THWAVE-SERVER.7thwave.local> Message-ID: I love fishing - hate the worms, tho. Anyway, can someone please advise me on the best fixative for nerve tissue which is going to be processed in GMA? Thanks, Jackie From JWEEMS <@t> sjha.org Mon Apr 7 15:51:08 2008 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Mon Apr 7 15:51:14 2008 Subject: [Histonet] Surgipath's O-fix for breast fixation. In-Reply-To: <704535.31547.qm@web65706.mail.ac4.yahoo.com> References: <704535.31547.qm@web65706.mail.ac4.yahoo.com> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD320518EC92@sjhaexc02.sjha.org> And the most important aspect is that a patient can be denied a clinical trial if this protocol is not followed specifically, j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, April 07, 2008 4:47 PM To: JOHN P COLEMAN; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Surgipath's O-fix for breast fixation. When a protocol is approved, it is so to be used as approved and does not allow deviations, IF you want to avoid any payment problems. It has nothing to do with adequacy, but with fully following the protocol, regardless of any consideration. What is accepted is NBF, nothing else. Ren? J. JOHN P COLEMAN wrote: We routinely use Surgipath's O-fix on breast tissue for subsequent Her-2 testing via IHC and FISH. Is this now disallowed? According to the labeling, O-fix is a formalin based fixative with ethanol and acetic acid added. I would consider it an alcohol based fixative and disallowed only in the absence of the formalin. John P.J. Coleman HT(ASCP)QIHC Clinical Specialist Anatomic Pathology Sentara Laboratory Services Cell/Voicemail(757)335-2159 pager: (757)456-6695 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- You rock. That's why Blockbuster's offering you one month of Blockbuster Total Access, No Cost. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From jkiernan <@t> uwo.ca Mon Apr 7 16:06:24 2008 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Mon Apr 7 16:06:29 2008 Subject: [Histonet] Alcian Blue-Metanil Yellow In-Reply-To: References: Message-ID: I haven't used the combination but have used both these dyes in other outlined belo yellow don't keep v brown over a couple of wee new solution. Could deteriorat your difficulty?
 < blue can, with time, change into an insolu can happen to to the stored powder, which to dissolve. The change occurs quite quickly at about pH 5 that are used in conjunction with a magnesiu The more acidic alcian blue solutions (pH 1 or 2.5) commonly for staining are usually stable for several months.
&nb sp;
Alcian blue 8G is available as a certified dye, so don' adequately tested by the Commission.
 
John KiernanAnatomy, UWO
London, Canada 
= = =< <dm April 3, 200 Blue-Metanil Yellow< histonet@lists.utsouthwestern.edu

> anyone using this stain.  We were doing this stain
biopsies. 
> programmed on>  
> worked great but recently we have been experiencing problems< BR>> with uneven, inconsistent staining.  Has anyo stain? ___________ _______________________ 5F mailing li Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rchiovetti <@t> yahoo.com Mon Apr 7 19:38:31 2008 From: rchiovetti <@t> yahoo.com (Robert Chiovetti) Date: Mon Apr 7 19:38:36 2008 Subject: [Histonet] Sliding microtome blade, a-profile Message-ID: <30506.37990.qm@web58913.mail.re1.yahoo.com> Bob, Boy, it's been *forever* since I've seen an a-profile blade. If you get in a bind, you might want to talk to the folks at DelawareDiamond Knives (800-222-5143). Besides diamond tools and knives, thecan also help with special profile tungsten carbide blades... at least they used to do this. You mighthave to send them your knife as an example so they can measure it, andI'm sure it won't be cheap. But it might be worth a try. Their website is www.ddk.com. Good Luck! Bob Chiovetti Robert (Bob) Chiovetti, Ph.D. Southwest Precision Instruments See What's New on Our Website! Arizona's Microscopy Resource 132 North Elster Drive Tucson, AZ 85710-3212 Tel./Fax 520-546-4986 Member, Arizona Small Business Association (www.asba.com) ----- Original Message ---- From: Bob Nienhuis To: Histonet Sent: Monday, April 7, 2008 10:51:48 AM Subject: [Histonet] Sliding microtome blade, a-profile Can anyone point me to a source for an a-profile blade for an AO 860 sliding microtome? FYI, the a-profile blades had a concave upper surface and a flat lower surface. Generally used for cutting celloidin sections. Bob Nienhuis Neurobiology Research, M/S 151A3 UCLA / VA Medical Center North Hills, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ You rock. That's why Blockbuster's offering you one month of Blockbuster Total Access, No Cost. http://tc.deals.yahoo.com/tc/blockbuster/text5.com From san.htin <@t> yahoo.com Tue Apr 8 00:33:05 2008 From: san.htin <@t> yahoo.com (San Tin) Date: Tue Apr 8 00:33:10 2008 Subject: [Histonet] Oct3/4 Message-ID: <957545.30792.qm@web55805.mail.re3.yahoo.com> Dear All, Kindly advice me the _________________________________________________________________ You rock. That's why Blockbuster's offering you [1]one month of Blockbuster Total Access, No Cost. References 1. http://us.rd.yahoo.com/evt=47523/*http://tc.deals.yahoo.com/tc/blockbuster/text5.com From san.htin <@t> yahoo.com Tue Apr 8 00:34:10 2008 From: san.htin <@t> yahoo.com (San Tin) Date: Tue Apr 8 00:34:13 2008 Subject: [Histonet] Oct3/4 Message-ID: <254833.82321.qm@web55802.mail.re3.yahoo.com> Dear All, kindly advice me of the vendor for oct 3/4 antibody. Thanks San _________________________________________________________________ You rock. That's why Blockbuster's offering you [1]one month of Blockbuster Total Access, No Cost. References 1. http://us.rd.yahoo.com/evt=47523/*http://tc.deals.yahoo.com/tc/blockbuster/text5.com From marktarango <@t> gmail.com Tue Apr 8 00:53:41 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Tue Apr 8 00:53:47 2008 Subject: [Histonet] Oct3/4 In-Reply-To: <254833.82321.qm@web55802.mail.re3.yahoo.com> References: <254833.82321.qm@web55802.mail.re3.yahoo.com> Message-ID: <5b6eb13e0804072253r26fad55fyc1be6fed2af84a80@mail.gmail.com> Biocare has one that works on formalin-fixed paraffin-embedded tissue. I've used it and it does work. The best control is probably a germ cell tumor or seminoma. The stain is, of course, nuclear. I've seen some scattered cell stain positive in normal bone marrow too. Let me know if you need a protocol. On Mon, Apr 7, 2008 at 10:34 PM, San Tin wrote: > > Dear All, > > kindly advice me of the vendor for oct 3/4 antibody. > > Thanks > > San > _________________________________________________________________ > > You rock. That's why Blockbuster's offering you [1]one month of > Blockbuster Total Access, No Cost. > > References > > 1. > http://us.rd.yahoo.com/evt=47523/*http://tc.deals.yahoo.com/tc/blockbuster/text5.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From CBraaten <@t> Cheshire-Med.COM Tue Apr 8 04:26:06 2008 From: CBraaten <@t> Cheshire-Med.COM (Christine I. Braaten) Date: Tue Apr 8 04:31:27 2008 Subject: [Histonet] biogenex xmatrix Message-ID: Good morning all, I was just wondering if there is anyone out there that uses the xmatrix from Biogenex and if I could get some feedback. We currently have one in house for a demo. Apparently there is a newer model ready to hit the market. I have heard that they cannot live up to their claims. Thanks in advance. Christine CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. From Dorothy.L.Webb <@t> HealthPartners.Com Tue Apr 8 09:26:24 2008 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Tue Apr 8 09:26:32 2008 Subject: [Histonet] Fading of slides Message-ID: <0E394B648E5284478A6CCB78E5AFDA27056355E9@hpes1.HealthPartners.int> We have noticed some fading of H&E stained slides, very sporadic, since the first of this year. Would anyone have any ideas as to the possible cause? We use the coverslipping tape or film and have for many years now. If this was the problem, I do not understand why all of the slides wouldn't be fading. Help and thanks!!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From sonya.martin <@t> soton.ac.uk Tue Apr 8 09:39:56 2008 From: sonya.martin <@t> soton.ac.uk (James S.) Date: Tue Apr 8 09:40:09 2008 Subject: [Histonet] Formalin fixation - how long does it last? Message-ID: <71437982F5B13A4D9A5B2669BDB89EE416F8A1A1@ISS-CL-EX-V1.soton.ac.uk> I have been given some small mouse bones which were fixed in 10% formalin for 48h but were then transferred to EDTA for decalcification. They have been left in the EDTA for about 6 weeks. Will they still be fixed or should I put them back into formalin - or is it too late to do anything with them? Ideally we want to stain for B cells but could just do some H&E if we cant get any staining. Thanks Sonya From rjbuesa <@t> yahoo.com Tue Apr 8 09:43:36 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Apr 8 09:43:46 2008 Subject: [Histonet] Fishing for GMA info In-Reply-To: Message-ID: <103582.95047.qm@web65701.mail.ac4.yahoo.com> NBF Ren? J. Jackie M O'Connor wrote: I love fishing - hate the worms, tho. Anyway, can someone please advise me on the best fixative for nerve tissue which is going to be processed in GMA? Thanks, Jackie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- You rock. That's why Blockbuster's offering you one month of Blockbuster Total Access, No Cost. From ratliffjack <@t> hotmail.com Tue Apr 8 09:57:39 2008 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Tue Apr 8 09:57:44 2008 Subject: [Histonet] Sliding microtome blade, a-profile In-Reply-To: <30506.37990.qm@web58913.mail.re1.yahoo.com> References: <30506.37990.qm@web58913.mail.re1.yahoo.com> Message-ID: You could also compare pricing of at Dorn and Hart (www.dornandhart.com). They offer special profile tungsten carbide blades too. Jack > Date: Mon, 7 Apr 2008 17:38:31 -0700> From: rchiovetti@yahoo.com> To: bob.nienhuis@gmail.com; histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Sliding microtome blade, a-profile> CC: > > Bob,> > Boy, it's been *forever* since I've seen an a-profile blade.> > If you get in a bind, you might want to talk to the folks at DelawareDiamond Knives (800-222-5143). Besides diamond tools and knives, thecan also help with special profile tungsten carbide blades... at least they used to do this. You mighthave to send them your knife as an example so they can measure it, andI'm sure it won't be cheap. But it might be worth a try. Their website is www.ddk.com.> > Good Luck!> > Bob Chiovetti > Robert (Bob) Chiovetti, Ph.D.> Southwest Precision Instruments> See What's New on Our Website!> Arizona's Microscopy Resource> 132 North Elster Drive> Tucson, AZ 85710-3212> Tel./Fax 520-546-4986> Member, Arizona Small Business Association> (www.asba.com)> > ----- Original Message ----> From: Bob Nienhuis > To: Histonet > Sent: Monday, April 7, 2008 10:51:48 AM> Subject: [Histonet] Sliding microtome blade, a-profile> > Can anyone point me to a source for an a-profile blade> for an AO 860 sliding microtome?> > FYI, the a-profile blades had a concave upper surface> and a flat lower surface. Generally used for cutting> celloidin sections.> > Bob Nienhuis> Neurobiology Research, M/S 151A3> UCLA / VA Medical Center> North Hills, CA> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > > > > ____________________________________________________________________________________> You rock. That's why Blockbuster's offering you one month of Blockbuster Total Access, No Cost. > http://tc.deals.yahoo.com/tc/blockbuster/text5.com> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Use video conversation to talk face-to-face with Windows Live Messenger. http://www.windowslive.com/messenger/connect_your_way.html?ocid=TXT_TAGLM_WL_Refresh_messenger_video_042008 From JBower <@t> hei.org Tue Apr 8 09:58:03 2008 From: JBower <@t> hei.org (Bower, Jennifer) Date: Tue Apr 8 09:58:08 2008 Subject: [Histonet] Formalin fixation - how long does it last? In-Reply-To: <71437982F5B13A4D9A5B2669BDB89EE416F8A1A1@ISS-CL-EX-V1.soton.ac.uk> References: <71437982F5B13A4D9A5B2669BDB89EE416F8A1A1@ISS-CL-EX-V1.soton.ac.uk> Message-ID: <87449E4A2B01DA47B29424CE5D6E0F8308076D30@hi0sml1.hei.org> We use a 2% formalin/125mM EDTA solution to do our mouse bone decalcification. Are you sure there wasn't any formalin in with the EDTA? I'm pretty sure you can't "unfix" formalin fixation, if the bone is decalcified to your liking, continue with your protocol and see if you like the results. Jennifer -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James S. Sent: Tuesday, April 08, 2008 7:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin fixation - how long does it last? I have been given some small mouse bones which were fixed in 10% formalin for 48h but were then transferred to EDTA for decalcification. They have been left in the EDTA for about 6 weeks. Will they still be fixed or should I put them back into formalin - or is it too late to do anything with them? Ideally we want to stain for B cells but could just do some H&E if we cant get any staining. Thanks Sonya _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dphillips <@t> vetmed.lsu.edu Tue Apr 8 09:58:47 2008 From: dphillips <@t> vetmed.lsu.edu (del phillips) Date: Tue Apr 8 09:59:50 2008 Subject: [Histonet] remove from list Message-ID: <000001c89989$0e6c7fa0$2b457ee0$@lsu.edu> Please remove me from this list DEL PHILLIPS From Barry.R.Rittman <@t> uth.tmc.edu Tue Apr 8 10:44:13 2008 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Tue Apr 8 10:44:16 2008 Subject: [Histonet] Formalin fixation - how long does it last? In-Reply-To: <87449E4A2B01DA47B29424CE5D6E0F8308076D30@hi0sml1.hei.org> References: <71437982F5B13A4D9A5B2669BDB89EE416F8A1A1@ISS-CL-EX-V1.soton.ac.uk> <87449E4A2B01DA47B29424CE5D6E0F8308076D30@hi0sml1.hei.org> Message-ID: Sonya Generally you can see some maceration of the tissue if the formalin bonds start to be broken down. This generally occurs with large pieces of bone and teeth if demineralization in EDTA is continued for a lengthy period. Can then just place in fresh formalin solution for a short time and then continue with the demineralization. This is assuming that the original formalin fixation was sufficient. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James S. Sent: Tuesday, April 08, 2008 7:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin fixation - how long does it last? I have been given some small mouse bones which were fixed in 10% formalin for 48h but were then transferred to EDTA for decalcification. They have been left in the EDTA for about 6 weeks. Will they still be fixed or should I put them back into formalin - or is it too late to do anything with them? Ideally we want to stain for B cells but could just do some H&E if we cant get any staining. Thanks Sonya _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From contact <@t> excaliburpathology.com Tue Apr 8 11:03:07 2008 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Tue Apr 8 11:03:18 2008 Subject: [Histonet] Fading of slides Message-ID: <133134.68361.qm@web50112.mail.re2.yahoo.com> Check that you are rinsing well after the bluing solution.?Incomplete removal will cause eosin to fade with time. ?Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 631 N. Broadway Ave. Moore, OK 73160 405-759-3953 lab contact@excaliburpathology.com www.excaliburpathology.com From rjbuesa <@t> yahoo.com Tue Apr 8 14:44:33 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Apr 8 14:44:41 2008 Subject: [Histonet] Fading of slides In-Reply-To: <0E394B648E5284478A6CCB78E5AFDA27056355E9@hpes1.HealthPartners.int> Message-ID: <114115.56489.qm@web65701.mail.ac4.yahoo.com> Since it is something recently started, try to contact your water supply treatment plant, they may have increased the chlorination level, and that could be the culprit. Ren? J. "Webb, Dorothy L" wrote: --------------------------------- You rock. That's why Blockbuster's offering you one month of Blockbuster Total Access, No Cost. From vd38 <@t> georgetown.edu Tue Apr 8 16:32:26 2008 From: vd38 <@t> georgetown.edu (Vernon Dailey) Date: Tue Apr 8 16:32:38 2008 Subject: [Histonet] Nuclear Envelope/Membrane Message-ID: <1bc8cc1c0fe3.1c0fe31bc8cc@imap.georgetown.edu> Hi Histonet, I need to know if there is a chemical stain specific to the nuclear membrane....it could also stain the plasma membrane as long as good nuclear membrane staining is present. any info would be appreciated. i am aware of immuno appraches such as lamin B etc....chemical stain would be best...thanks again. Vernon Dailey From arunams <@t> interchange.ubc.ca Wed Apr 9 10:23:27 2008 From: arunams <@t> interchange.ubc.ca (Aruna Somasiri) Date: Wed Apr 9 10:23:34 2008 Subject: [Histonet] Staining JB-4 sections Message-ID: <64127.961207754607934.JavaMail.myubc2@handel.my.ubc.ca> Hi Everyone, I am having problems getting good Movats and Trichrom staining on JB-4 sections. If some one have a good protocol to share much appreciated. Thanks in advance for your help. Regards Aruna From mona_diane <@t> hotmail.com Wed Apr 9 11:20:45 2008 From: mona_diane <@t> hotmail.com (Ramona Turner) Date: Wed Apr 9 11:20:48 2008 Subject: [Histonet] Immunohistochemistry Machine Message-ID: I am looking for a machine to process immunohistochemistry stains. I want something small, that will do as much online as possible with little hands on attention required. Any recommendations? Ramona Potomac Hospital _________________________________________________________________ Going green? See the top 12 foods to eat organic. http://green.msn.com/galleries/photos/photos.aspx?gid=164&ocid=T003MSN51N1653A From williamstewart.pathology <@t> gmail.com Wed Apr 9 11:27:09 2008 From: williamstewart.pathology <@t> gmail.com (William Stewart) Date: Wed Apr 9 11:27:13 2008 Subject: [Histonet] Immunohistochemistry Machine In-Reply-To: References: Message-ID: <81a8916f0804090927g5c10f3c5y5b684d4e25147976@mail.gmail.com> It is not small, but will do all steps of staining for MOST antibodies. Try Ventana Benchmark LT or XT. Bill On 4/9/08, Ramona Turner wrote: > > > > > I am looking for a machine to process immunohistochemistry stains. I want > something small, that will do as much online as possible with little hands > on attention required. Any recommendations? > > Ramona > Potomac Hospital > _________________________________________________________________ > Going green? See the top 12 foods to eat organic. > > http://green.msn.com/galleries/photos/photos.aspx?gid=164&ocid=T003MSN51N1653A_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From doug <@t> ppspath.com Wed Apr 9 12:39:35 2008 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Wed Apr 9 11:40:57 2008 Subject: SPAM-LOW: [Histonet] Immunohistochemistry Machine In-Reply-To: Message-ID: Romona, Ventana NexES IHC. It is small and fully automated. http://www.ventanamed.com/products/instruments/nexes_ihc.html If you have a little more space, take a look at the BenchMark XT. http://www.ventanamed.com/products/instruments/xt.html Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ramona Turner Sent: Wednesday, April 09, 2008 11:21 AM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] Immunohistochemistry Machine I am looking for a machine to process immunohistochemistry stains. I want something small, that will do as much online as possible with little hands on attention required. Any recommendations? Ramona Potomac Hospital _________________________________________________________________ Going green? See the top 12 foods to eat organic. http://green.msn.com/galleries/photos/photos.aspx?gid=164&ocid=T003MSN51N165 3A_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Wed Apr 9 11:44:38 2008 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Wed Apr 9 11:44:47 2008 Subject: SPAM-LOW: [Histonet] Immunohistochemistry Machine In-Reply-To: <20080409164116.7599F200B1@mailsec1-in.hosp.wisc.edu> Message-ID: Caution about the NexES IHC module; I don't believe that Ventana is making it anymore although they may still be selling it. You wouldn't want to get in a situation where its not being supported anymore. VMS can correct me if I'm wrong about this. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Wednesday, April 09, 2008 12:40 PM To: 'Ramona Turner'; histonet@lists.utsouthwestern.edu Subject: RE: SPAM-LOW: [Histonet] Immunohistochemistry Machine Romona, Ventana NexES IHC. It is small and fully automated. http://www.ventanamed.com/products/instruments/nexes_ihc.html If you have a little more space, take a look at the BenchMark XT. http://www.ventanamed.com/products/instruments/xt.html Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ramona Turner Sent: Wednesday, April 09, 2008 11:21 AM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] Immunohistochemistry Machine I am looking for a machine to process immunohistochemistry stains. I want something small, that will do as much online as possible with little hands on attention required. Any recommendations? Ramona Potomac Hospital _________________________________________________________________ Going green? See the top 12 foods to eat organic. http://green.msn.com/galleries/photos/photos.aspx?gid=164&ocid=T003MSN51 N165 3A_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Wed Apr 9 12:31:30 2008 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Apr 9 12:31:49 2008 Subject: [Histonet] Immunohistochemistry Machine In-Reply-To: References: Message-ID: <47FCC532020000770000BE42@gwmail4.harthosp.org> I really like Leica-Microsystems' "Bond Max". Obviously, the selection of an automated platform will depend on your technical staff's IHC experience, the number of IHC stains that you perform, and your budget. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> Ramona Turner 04/09/08 12:20 PM >>> I am looking for a machine to process immunohistochemistry stains. I want something small, that will do as much online as possible with little hands on attention required. Any recommendations? Ramona Potomac Hospital _________________________________________________________________ Going green? See the top 12 foods to eat organic. http://green.msn.com/galleries/photos/photos.aspx?gid=164&ocid=T003MSN51N1653A_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From John.Spair <@t> multicare.org Wed Apr 9 12:14:58 2008 From: John.Spair <@t> multicare.org (John Spair) Date: Wed Apr 9 12:41:19 2008 Subject: [Histonet] RE: Histonet Digest, Vol 53, Issue 14 In-Reply-To: <6BE229FD6H8321911-01@MMS_multicare.org> References: <6BE229FD6H8321911-01@MMS_multicare.org> Message-ID: <61A9977919846C479389493BAE2517CA02223E82@MHSEXMBX1.multicare.org> I was told the Ventana NexES is not sold any longer, as I tried to buy one. Ended up buying the XT. "MMS " made the following annotations. ------------------------------------------------------------------------------ NOTICE: This e-mail and the attachments hereto, if any, may contain privileged and/or confidential information. It is intended only for use by the named addressee(s). If you are not the intended recipient of this e-mail, you are hereby notified that any examination, distribution or copying of this e-mail and the attachments hereto, if any, is strictly prohibited. If you have received this transmission in error, please immediately notify the sender by email or telephone and permanently delete this e-mail and the attachments hereto, if any, and destroy any printout thereof. MultiCare Health System, Tacoma, WA 98415 (253) 403-1000. ============================================================================== From Susan.Weber2 <@t> va.gov Wed Apr 9 13:40:06 2008 From: Susan.Weber2 <@t> va.gov (Weber, Susan (VHACLE)) Date: Wed Apr 9 13:40:12 2008 Subject: [Histonet] Immunohistochemistry Machine In-Reply-To: References: Message-ID: <16C83872A53F4346AA9C3A18E3A3AAB903F76D65@VHAV10MSGA1.v10.med.va.gov> Love the Ventana Benchmark XT. Very hands-off, Very user friendly. They come initially come out to the site to Optimize your protocols and also have a very good training program (especially for performing maintenance). I have also found their tech support to be great too. Just my 2 cents! Susan M Weber HT(ASCP) Histology Supervisor Louis Stokes Cleveland VA Medical Center 10701 East Blvd Cleveland, Ohio 44106 (216) 791-3800 X6154 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ramona Turner Sent: Wednesday, April 09, 2008 12:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Immunohistochemistry Machine I am looking for a machine to process immunohistochemistry stains. I want something small, that will do as much online as possible with little hands on attention required. Any recommendations? Ramona Potomac Hospital _________________________________________________________________ Going green? See the top 12 foods to eat organic. http://green.msn.com/galleries/photos/photos.aspx?gid=164&ocid=T003MSN51 N1653A_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Eric.Mahoney <@t> cchmc.org Wed Apr 9 13:58:15 2008 From: Eric.Mahoney <@t> cchmc.org (Eric Mahoney) Date: Wed Apr 9 13:58:47 2008 Subject: [Histonet] Re: Nuclear Envelope/Membrane (Vernon Dailey) Message-ID: <47FCD987020000970001343C@n6mcgw16.cchmc.org> Use POPO3 Iodide, it is a nuclear membrane stain. 1:4000 concentration. 1uL Popo3 : 4000uL 1xPBS >>> 04/09/08 12:59 PM >>> Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Fading of slides (Rene J Buesa) 2. Nuclear Envelope/Membrane (Vernon Dailey) 3. Staining JB-4 sections (Aruna Somasiri) 4. Immunohistochemistry Machine (Ramona Turner) 5. Re: Immunohistochemistry Machine (William Stewart) 6. RE: SPAM-LOW: [Histonet] Immunohistochemistry Machine (Douglas D Deltour) 7. RE: SPAM-LOW: [Histonet] Immunohistochemistry Machine (Sebree Linda A.) ---------------------------------------------------------------------- Message: 1 Date: Tue, 8 Apr 2008 12:44:33 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Fading of slides To: "Webb, Dorothy L" , histonet@lists.utsouthwestern.edu Message-ID: <114115.56489.qm@web65701.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Since it is something recently started, try to contact your water supply treatment plant, they may have increased the chlorination level, and that could be the culprit. Ren? J. "Webb, Dorothy L" wrote: --------------------------------- You rock. That's why Blockbuster's offering you one month of Blockbuster Total Access, No Cost. ------------------------------ Message: 2 Date: Tue, 08 Apr 2008 17:32:26 -0400 From: Vernon Dailey Subject: [Histonet] Nuclear Envelope/Membrane To: histonet@lists.utsouthwestern.edu Message-ID: <1bc8cc1c0fe3.1c0fe31bc8cc@imap.georgetown.edu> Content-Type: text/plain; charset=us-ascii Hi Histonet, I need to know if there is a chemical stain specific to the nuclear membrane....it could also stain the plasma membrane as long as good nuclear membrane staining is present. any info would be appreciated. i am aware of immuno appraches such as lamin B etc....chemical stain would be best...thanks again. Vernon Dailey ------------------------------ Message: 3 Date: Wed, 09 Apr 2008 08:23:27 -0700 (PDT) From: Aruna Somasiri Subject: [Histonet] Staining JB-4 sections To: histonetlistsutsouthwesternedu Message-ID: <64127.961207754607934.JavaMail.myubc2@handel.my.ubc.ca> Content-Type: text/plain; charset=us-ascii Hi Everyone, I am having problems getting good Movats and Trichrom staining on JB-4 sections. If some one have a good protocol to share much appreciated. Thanks in advance for your help. Regards Aruna ------------------------------ Message: 4 Date: Wed, 9 Apr 2008 12:20:45 -0400 From: Ramona Turner Subject: [Histonet] Immunohistochemistry Machine To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" I am looking for a machine to process immunohistochemistry stains. I want something small, that will do as much online as possible with little hands on attention required. Any recommendations? Ramona Potomac Hospital _________________________________________________________________ Going green? See the top 12 foods to eat organic. http://green.msn.com/galleries/photos/photos.aspx?gid=164&ocid=T003MSN51N1653A ------------------------------ Message: 5 Date: Wed, 9 Apr 2008 12:27:09 -0400 From: "William Stewart" Subject: Re: [Histonet] Immunohistochemistry Machine To: "Ramona Turner" Cc: histonet@lists.utsouthwestern.edu Message-ID: <81a8916f0804090927g5c10f3c5y5b684d4e25147976@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 It is not small, but will do all steps of staining for MOST antibodies. Try Ventana Benchmark LT or XT. Bill On 4/9/08, Ramona Turner wrote: > > > > > I am looking for a machine to process immunohistochemistry stains. I want > something small, that will do as much online as possible with little hands > on attention required. Any recommendations? > > Ramona > Potomac Hospital > _________________________________________________________________ > Going green? See the top 12 foods to eat organic. > > http://green.msn.com/galleries/photos/photos.aspx?gid=164&ocid=T003MSN51N1653A_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 6 Date: Wed, 9 Apr 2008 12:39:35 -0500 From: "Douglas D Deltour" Subject: RE: SPAM-LOW: [Histonet] Immunohistochemistry Machine To: "'Ramona Turner'" , Message-ID: Content-Type: text/plain; charset="us-ascii" Romona, Ventana NexES IHC. It is small and fully automated. http://www.ventanamed.com/products/instruments/nexes_ihc.html If you have a little more space, take a look at the BenchMark XT. http://www.ventanamed.com/products/instruments/xt.html Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ramona Turner Sent: Wednesday, April 09, 2008 11:21 AM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] Immunohistochemistry Machine I am looking for a machine to process immunohistochemistry stains. I want something small, that will do as much online as possible with little hands on attention required. Any recommendations? Ramona Potomac Hospital _________________________________________________________________ Going green? See the top 12 foods to eat organic. http://green.msn.com/galleries/photos/photos.aspx?gid=164&ocid=T003MSN51N165 3A_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Wed, 9 Apr 2008 11:44:38 -0500 From: "Sebree Linda A." Subject: RE: SPAM-LOW: [Histonet] Immunohistochemistry Machine To: "Douglas D Deltour" , "Ramona Turner" , Message-ID: Content-Type: text/plain; charset="US-ASCII" Caution about the NexES IHC module; I don't believe that Ventana is making it anymore although they may still be selling it. You wouldn't want to get in a situation where can correct me if I'm wrong about this. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Wednesday, April 09, 2008 12:40 PM To: 'Ramona Turner'; histonet@lists.utsouthwestern.edu Subject: RE: SPAM-LOW: [Histonet] Immunohistochemistry Machine Romona, Ventana NexES IHC. It is small and fully automated. http://www.ventanamed.com/products/instruments/nexes_ihc.html If you have a little more space, take a look at the BenchMark XT. http://www.ventanamed.com/products/instruments/xt.html Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ramona Turner Sent: Wednesday, April 09, 2008 11:21 AM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] Immunohistochemistry Machine I am looking for a machine to process immunohistochemistry stains. I want something small, that will do as much online as possible with little hands on attention required. Any recommendations? Ramona Potomac Hospital _________________________________________________________________ Going green? See the top 12 foods to eat organic. http://green.msn.com/galleries/photos/photos.aspx?gid=164&ocid=T003MSN51 N165 3A_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 53, Issue 14 **************************************** From JMahoney <@t> alegent.org Wed Apr 9 14:02:05 2008 From: JMahoney <@t> alegent.org (Mahoney,Janice A) Date: Wed Apr 9 14:02:36 2008 Subject: [Histonet] Immunohistochemistry Machine In-Reply-To: <16C83872A53F4346AA9C3A18E3A3AAB903F76D65@VHAV10MSGA1.v10.med.va.gov> References: , <16C83872A53F4346AA9C3A18E3A3AAB903F76D65@VHAV10MSGA1.v10.med.va.gov> Message-ID: <346E5878979BA54FB4B0BFD6AD93B9B9B01EE96229@EXCHMBC1.ad.ah.local> Same here. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weber, Susan (VHACLE) [Susan.Weber2@va.gov] Sent: Wednesday, April 09, 2008 1:40 PM To: Ramona Turner; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Immunohistochemistry Machine Love the Ventana Benchmark XT. Very hands-off, Very user friendly. They come initially come out to the site to Optimize your protocols and also have a very good training program (especially for performing maintenance). I have also found their tech support to be great too. Just my 2 cents! Susan M Weber HT(ASCP) Histology Supervisor Louis Stokes Cleveland VA Medical Center 10701 East Blvd Cleveland, Ohio 44106 (216) 791-3800 X6154 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ramona Turner Sent: Wednesday, April 09, 2008 12:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Immunohistochemistry Machine I am looking for a machine to process immunohistochemistry stains. I want something small, that will do as much online as possible with little hands on attention required. Any recommendations? Ramona Potomac Hospital _________________________________________________________________ Going green? See the top 12 foods to eat organic. http://green.msn.com/galleries/photos/photos.aspx?gid=164&ocid=T003MSN51 N1653A_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Janice mahoney HT(ASCP) Histology/Cytology Coordinator Alegent Health Laboratory 4955 F Street Omaha, NE (402)717-2889 fax(402)717-5231 Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From roosmith1 <@t> hotmail.com Wed Apr 9 14:16:02 2008 From: roosmith1 <@t> hotmail.com (The Smith's) Date: Wed Apr 9 14:16:06 2008 Subject: [Histonet] Immunohistochemistry Machine In-Reply-To: References: Message-ID: Ramona... I would additionally look into a new system from Celerus Diagnostics called 'The Wave'. A new continuous feed IHC stainer with 15 minute turn around time with walk away automation. It has an extremely small footprint as well...worth looking into if you are just starting your 'shopping'!! Visit www.celerusdiagnositc.com/wave Good Luck, Jackie Smith > From: mona_diane@hotmail.com> To: histonet@lists.utsouthwestern.edu> Date: Wed, 9 Apr 2008 12:20:45 -0400> Subject: [Histonet] Immunohistochemistry Machine> > > > > I am looking for a machine to process immunohistochemistry stains. I want something small, that will do as much online as possible with little hands on attention required. Any recommendations?> > Ramona> Potomac Hospital> _________________________________________________________________> Going green? See the top 12 foods to eat organic.> http://green.msn.com/galleries/photos/photos.aspx?gid=164&ocid=T003MSN51N1653A_______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Use video conversation to talk face-to-face with Windows Live Messenger. http://www.windowslive.com/messenger/connect_your_way.html?ocid=TXT_TAGLM_WL_Refresh_messenger_video_042008 From godsgalnow <@t> aol.com Wed Apr 9 14:22:14 2008 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Wed Apr 9 14:22:24 2008 Subject: [Histonet] Immunohistochemistry Machine In-Reply-To: References: Message-ID: <8CA688E26E5A3D4-8BC-220C@WEBMAIL-DC11.sysops.aol.com> The scientist in me prefers an open platform, like the Nemeis or The IntelliPath.? I don't think I could ever relinquish full control to a machine. Roxanne -----Original Message----- From: Ramona Turner To: histonet@lists.utsouthwestern.edu Sent: Wed, 9 Apr 2008 12:20 pm Subject: [Histonet] Immunohistochemistry Machine I am looking for a machine to process immunohistochemistry stains. I want something small, that will do as much online as possible with little hands on attention required. Any recommendations? Ramona Potomac Hospital _________________________________________________________________ Going green? See the top 12 foods to eat organic. http://green.msn.com/galleries/photos/photos.aspx?gid=164&ocid=T003MSN51N1653A_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From burch007 <@t> mc.duke.edu Wed Apr 9 14:55:00 2008 From: burch007 <@t> mc.duke.edu (James L Burchette) Date: Wed Apr 9 14:55:09 2008 Subject: [Histonet] Immunohistochemistry Machine In-Reply-To: Message-ID: Leica Bond Max hands down! Jim Burchette, HT(ASCP) QIHC "A simple histotech from a little country hospital in North Carolina" Ramona Turner Sent by: histonet-bounces@lists.utsouthwestern.edu 04/09/2008 12:20 PM To cc Subject [Histonet] Immunohistochemistry Machine I am looking for a machine to process immunohistochemistry stains. I want something small, that will do as much online as possible with little hands on attention required. Any recommendations? Ramona Potomac Hospital _________________________________________________________________ Going green? See the top 12 foods to eat organic. http://green.msn.com/galleries/photos/photos.aspx?gid=164&ocid=T003MSN51N1653A_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sweaver <@t> tvmdl.tamu.edu Wed Apr 9 15:25:29 2008 From: sweaver <@t> tvmdl.tamu.edu (Stephanie Weaver) Date: Wed Apr 9 15:25:52 2008 Subject: [Histonet] microwave processors Message-ID: <47FCDFE9.A3DC.00A0.0@tvmdl.tamu.edu> I am considering purchasing a microwave processor for our histo lab. I would like to be able to process up to 100 cassettes of various tissues (no nervous tissue and limited fatty tissues) in about 3 hours. I work in a veterinary diagnostic lab so budget is a factor. There is a large variety of options from a simple microwave with processing baskets, to the Tissue-Tek Xpress series and a huge range of prices as well. Is it possible to get decent quality with the modified lab microwaves? Does anyone have any advice or suggestions for me? Information from sales reps is welcome also. Thanks in advance, Stephanie From tkngflght <@t> yahoo.com Wed Apr 9 14:23:12 2008 From: tkngflght <@t> yahoo.com (Cheryl) Date: Wed Apr 9 15:29:02 2008 Subject: The Wave -questions RE: [Histonet] Immunohistochemistry Machine In-Reply-To: References: Message-ID: <01b901c89a77$2f7d5db0$6901a8c0@FSROGER> Hi everyone-- Does anyone have direct experience or has anyone seen this in operation? If it does what they claim--WOW! Any information (including vendor response) is welcome-- http://www.celerusdiagnostics.com/wave Thanks! Cheryl Cheryl R. Kerry, HT(ASCP), BA Full Staff Inc. Staffing the lab - One GREAT tech at a time. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone admin@fullstaff.org www.fullstaff.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of The Smith's Sent: Wednesday, April 09, 2008 2:16 PM To: Ramona Turner; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Immunohistochemistry Machine Ramona... I would additionally look into a new system from Celerus Diagnostics called 'The Wave'. A new continuous feed IHC stainer with 15 minute turn around time with walk away automation. It has an extremely small footprint as well...worth looking into if you are just starting your 'shopping'!! Visit www.celerusdiagnositc.com/wave Good Luck, Jackie Smith > From: mona_diane@hotmail.com> To: histonet@lists.utsouthwestern.edu> > Date: Wed, 9 Apr 2008 12:20:45 -0400> Subject: [Histonet] > Immunohistochemistry Machine> > > > > I am looking for a machine to > process immunohistochemistry stains. I want something small, that will > do as much online as possible with little hands on attention required. > Any recommendations?> > Ramona> Potomac Hospital> > _________________________________________________________________> > Going green? See the top 12 foods to eat organic.> > http://green.msn.com/galleries/photos/photos.aspx?gid=164&ocid=T003MSN > 51N1653A_______________________________________________> Histonet > mailing list> Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Use video conversation to talk face-to-face with Windows Live Messenger. http://www.windowslive.com/messenger/connect_your_way.html?ocid=TXT_TAGLM_WL _Refresh_messenger_video_042008_____________________________________________ __ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Wed Apr 9 16:52:23 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Apr 9 16:52:28 2008 Subject: The Wave -questions RE: [Histonet] Immunohistochemistry Machine In-Reply-To: <01b901c89a77$2f7d5db0$6901a8c0@FSROGER> References: <01b901c89a77$2f7d5db0$6901a8c0@FSROGER> Message-ID: <5b6eb13e0804091452m32243c55p945306babdeedaff@mail.gmail.com> I've never used it, but looking at the slides of the stains, it looks like they used a real negative control as an example (faint plasma cell staining). That's pretty honest. On Wed, Apr 9, 2008 at 12:23 PM, Cheryl wrote: > Hi everyone-- > > Does anyone have direct experience or has anyone seen this in operation? > If > it does what they claim--WOW! > > Any information (including vendor response) is welcome-- > > http://www.celerusdiagnostics.com/wave > > > Thanks! > > Cheryl > > > Cheryl R. Kerry, HT(ASCP), BA > > Full Staff Inc. > > Staffing the lab - One GREAT tech at a time. > > 281.852.9457 office > > 281.883.7704 cell > > 800.756.3309 fax and alternate phone > > admin@fullstaff.org > > www.fullstaff.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of The > Smith's > Sent: Wednesday, April 09, 2008 2:16 PM > To: Ramona Turner; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Immunohistochemistry Machine > > Ramona... > > I would additionally look into a new system from Celerus Diagnostics > called > 'The Wave'. A new continuous feed IHC stainer with 15 minute turn around > time with walk away automation. It has an extremely small footprint as > well...worth looking into if you are just starting your 'shopping'!! > > Visit www.celerusdiagnositc.com/wave > > > Good Luck, > Jackie Smith > > > > > From: mona_diane@hotmail.com> To: histonet@lists.utsouthwestern.edu> > > Date: Wed, 9 Apr 2008 12:20:45 -0400> Subject: [Histonet] > > Immunohistochemistry Machine> > > > > I am looking for a machine to > > process immunohistochemistry stains. I want something small, that will > > do as much online as possible with little hands on attention required. > > Any recommendations?> > Ramona> Potomac Hospital> > > _________________________________________________________________> > > Going green? See the top 12 foods to eat organic.> > > http://green.msn.com/galleries/photos/photos.aspx?gid=164&ocid=T003MSN > > 51N1653A_______________________________________________> Histonet > > mailing list> Histonet@lists.utsouthwestern.edu> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _________________________________________________________________ > Use video conversation to talk face-to-face with Windows Live Messenger. > > http://www.windowslive.com/messenger/connect_your_way.html?ocid=TXT_TAGLM_WL > > _Refresh_messenger_video_042008_____________________________________________ > __ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From anthony <@t> histotechexchange.com Wed Apr 9 17:41:46 2008 From: anthony <@t> histotechexchange.com (anthony@histotechexchange.com) Date: Wed Apr 9 18:04:26 2008 Subject: [Histonet] RE:Histonet Vacancies May I add my 2 cents worth? from Histotech Exchange LLC In-Reply-To: References: Message-ID: <1722.65.40.219.182.1207780906.squirrel@host7.wfdns.com> Dear Pam: For a long time histologists have gotten the short end of the stick. I believe that the current situation is a symptom of the problem and is due to a history of underpayment. We cannot solve the problem by artificial means. This country is based on the free market system. We must work with the system not against it, but first we must clearly understand the problem: Not enough techs, increasing barriers to entry into the profession, lack of visibility, no central planning to fix or even research the problem. We need to work to bring up the wages of HTs all across the country. If we do this, we will bring Histology to the attention of people in education at the moment. We can only get out of it by concentrating on increasing the number of facilities that are prepared to take on new graduates and spend the time to get their practical skills up to speed. I think you are doing a valuable job bringing this situation back to the surface. We need to keep the momentum up and not let it die on the vine of this valuable forum as a forgotten conversation. So, I ask my peers: What can we do to address this situation in an organized, effective manner? Who in our profession is responsible for being our cheerleader? Finally, Pam, I want to say for myself that I encourage all conversation. I will never be offended by a question, and I will always try to answer questions in a way that remembers there is a person behind it. No flame retardant needed here. I make my money from placing people on a temporary and permanent basis. I am also a Histologist first and foremost and believe that patient safety is number one and understaffed laboratories experience more mistakes. Keep up the good work, Pam. All my best, Anthony Williams BSc. HT Histotech Exchange LLC 19 Whitmore St. Lexington, VA 24450 T 1 (302) 383 9780 F 1 (540) 463 3583 anthony@histotechexchange.com > I know this is a problem that has plagued facilities for years and I too > have noticed a change in the past 2 years. Yes, the histology programs > nationwide produce a great albeit small group of talented people every > year but the pool of available histo techs for permanent positions has > shrunk even more in recent years. At the risk of being "flamed" by > travel companies I have to say that you are losing alot of techs to > travel positions. In the past 2 years of all of the histo techs I have > had contact with over half only want to work in permanent positions the > rest either want to continue as travelers or become travelers. Think > about it... they get a higher rate of pay, benefits and living expenses > paid for. For these people it is a "better deal" than committing to one > facility. As a matter of fact it is a "better deal" than a temp/travel > position in any other field outside of healthcare. Facilities who take > the "quick solution" of hiring travel techs are contributing to the > shortage. May I offer some solutions? Some creative hiring strategies? > > Here are some ideas I would like to share: > 1. If you are using travel techs do it with a temp to perm clause - but > be firm. If a tech works for you as a temp make sure they are at least > considering converting to a permanent employee at the end of the > contract. If not don't extend, have your travel company send someone > else who would consider converting to a permanent position. And make > questions about their intentions part of your interview process the same > as you would if you were interviewing a candidate from out of state for > a permanent position. > > 2. Human Resources - Many of your allied health recruiters don't seem to > realize that histo techs don't grow on trees. So many times I see > facilities lose great techs because the hiring process has dragged out > and the candidate ends up taking a position with a facility that can > move faster. Stay on top of your hr people especially once you know > they have a histology candidate. > > 3. How about techs from Canada? There are alot of talented techs in > Canada that are interested in moving to the states and the process is > relatively easy due to NAFTA and the F1 visa. > > 4. How about techs that need sponsorship on an H-1 visa? I know alot of > companies shy away from this alternative because of the length of time > it can take to process a visa application but I think that if you take a > look at the time it takes to find a tech at all against the time it > would take to process an H-1 visa it is quickly becoming 6 of one vs. > half dozen of another. I mean what difference does it make if it takes > up to 8 weeks to process an H-1 visa vs. 2-3 months to identify a > histology candidate? > > Your best bet is to get with your Human Resources department and > strategize, educate them on the challenges and shortages you are facing. > Discuss some of these options or others you might come up with. > I hope this helps!! > > Thank You! > > > Pam Barker > President > RELIA > Specialists in Allied Healthcare Recruiting > 5703 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell: (407)353-5070 > FAX: (407)678-2788 > E-mail: relia1@earthlink.net > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From amosbrooks <@t> gmail.com Wed Apr 9 19:07:32 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed Apr 9 19:07:58 2008 Subject: [Histonet] Gayle Callis Message-ID: <582736990804091707o4a9b2f5amea827389d6f7cdcf@mail.gmail.com> Hi, I'm sorry to waste bandwith for this, but could Gayle Callis please drop me a message off list. My apologies to everyone else. Thanks, Amos From hodges420 <@t> msn.com Wed Apr 9 19:22:10 2008 From: hodges420 <@t> msn.com (MARY T HODGES) Date: Wed Apr 9 19:22:15 2008 Subject: [Histonet] workload per person Message-ID: hello all, Can any one give me some guide lines on how many blocks someone should embed in the morning also how many blocks. Please take in consideration beginners,intermediate level and senior level techs Thanks, Tere Hodges. _________________________________________________________________ More immediate than e-mail? Get instant access with Windows Live Messenger. http://www.windowslive.com/messenger/overview.html?ocid=TXT_TAGLM_WL_Refresh_instantaccess_042008 From danindanville <@t> gmail.com Wed Apr 9 19:50:30 2008 From: danindanville <@t> gmail.com (Dan Dan) Date: Wed Apr 9 19:50:40 2008 Subject: [Histonet] Histonet Vacancies Message-ID: <69272a080804091750h66cd0ddbj9abf11863885e45e@mail.gmail.com> I'll add my two-cents worth. It's hard to believe that after 25 years in this business that the histotech "shortage" situation is pretty much the same as it was long ago. There have certainly been improvements in education and training of people in histology, but for the most part (99%??) people just fall into histology rather than pick it at some point in school. In fact, it is a very rare day when I meet someone who went to school for histotechnology, At this date there is still only one school on the west coast, and it is not the same school that existed for a while in Seattle! The new one is in southern california. I looked into starting a histotechnology course here in the SF Bay Area, but it takes a long while with lots of justification and scrounging for equipment and if I wanted to do it full time the pay would be half as much as I make now. I could do it part time but then I'd be working 60-70 hours a week rather than 50 I do now. For the time being I give presentations to the local junior colleges. I may expand that to high schools. I know they are interested in getting speakers, but finding the time... I work in the biotech field as an IHC lab manger. We don't require HT/HTL/QIHC (though would love to have them!)but it is still very difficult to get experienced histotechs and nearly impossible to get experienced IHC techs around here. Most of the people I interview are from the research field who may have done some IHC at some point. And the ones who have good IHC experience are making very high salaries that our company gags at matching. It is not possible to recruit out of the Bay Area because the cost of housing is so high that no one that is not already living here can afford to move here. Even getting someone from southern cal is difficult - justifiying relocation expenses is difficult. It is kind of a joke that the experienced IHC techs know each other and spend their careers trading places at the varioius companies. Anthony is quite right that it takes a lot of effort to educate HR and upper management about the need for higher pay. They really have a hard time justifying the same pay for a technologist that a new PhD will also make. On top of that we have loads of (legal) immigrants from Asia here and they are quite willing to work for lower pay to get a start. It is very common to have PhD level people, and even immigrant MD's (who can't get board certified here, mainly due to lanquage difficulties) working as lab techs. Who is going to hire a AA/BS histotech when they can get a PhD who is happy to do the work and able to contribute to R&D at a higher level than most techs? There is a huge disparity between what hospitals and private diagnostic labs pay. The local Kaiser labs and Univeristy hospital labs pay in the $35 per hour range for basic histology. Senior IHC techs are in the $45 range. Those places have unions which is why the pay is so high. On the other hand, private labs will pay experieinced,certified people almost that much, but new, inexperienced people may be paid $15-20/hr and the lab is not willing to help them get to their HT/HTL because they don't want to pay them more, and could lose them to the hospitals once trained. This is not speculation but things I have had told to me first hand from techs who work in these places. It is a little harder now for histotechs to be trained on the job due to the AA requirement. Most OJT people I have met were working in the hospital at some other job - lab assisant, dishwasher, etc before getting (accidently) into histology. A person working on an AA is probably not working in those jobs and probably does not even know a histology lab exists. Obviously the need is to get exposure for histotechnology. The NSH makes brochures and videos available and does a career day for high schools students at the national meeting every year (I've helped at those). This is a good foundation. But it will really take every state society and every interested histotech to do SOMETHING to promote the field. Science teachers at high schools are very interested in having working people come in to talk about a field. If someone in every city could do that it would go a long way towards promoting the field. Maybe the key is to have NSH come up with a standardised presentation that anyone can give along with some props to make it a tactile experience. But, after the promotion you have to realize that it takes a huge amount of initiative on the students part to follow through. They have to start work on, or finish, an AA /BS degree, make contact with a lab that is interested in training people, get the position, do on-the-job (maybe at the same time as school) and THEN work on learning the matieral for an HT/HTL pretty much alone. Their facing a 3 to 4 year process. Longer if they get a bachelors degree. Key is finding labs to training that value HT/HTL certification. Starting HT/HTL study groups would be a good way to get people motivated. This is the kind of thing a state society can do. Individuals can to it as well, but it helps to have a group for motivation. Of course, that is why it has not happened - it depends too much on a few super-motivated individuals. The schools don't hear of a demand and so do not have programs. No programs means students never hear about it. Since students don't hear about it they can't even consider it. A vicious circle. Imagine if the schools had students asking about courses leading to histotech certification or at least a chance at a job that leads to certification. That gets the schools attention. Junior colleges especially are very interested in providing trained people to the local job market. For them jobs = program justification. Think about it. Dan in Danville (a pseudonom to allow free speech) Dear Pam: For a long time histologists have gotten the short end of the stick. I believe that the current situation is a symptom of the problem and is due to a history of underpayment. We cannot solve the problem by artificial means. This country is based on the free market system. We must work with the system not against it, but first we must clearly understand the problem: Not enough techs, increasing barriers to entry into the profession, lack of visibility, no central planning to fix or even research the problem. We need to work to bring up the wages of HTs all across the country. If we do this, we will bring Histology to the attention of people in education at the moment. We can only get out of it by concentrating on increasing the number of facilities that are prepared to take on new graduates and spend the time to get their practical skills up to speed. I think you are doing a valuable job bringing this situation back to the surface. We need to keep the momentum up and not let it die on the vine of this valuable forum as a forgotten conversation. So, I ask my peers: What can we do to address this situation in an organized, effective manner? Who in our profession is responsible for being our cheerleader? Finally, Pam, I want to say for myself that I encourage all conversation. I will never be offended by a question, and I will always try to answer questions in a way that remembers there is a person behind it. No flame retardant needed here. I make my money from placing people on a temporary and permanent basis. I am also a Histologist first and foremost and believe that patient safety is number one and understaffed laboratories experience more mistakes. Keep up the good work, Pam. All my best, Anthony Williams BSc. HT Histotech Exchange LLC 19 Whitmore St. Lexington, VA 24450 T 1 (302) 383 9780 F 1 (540) 463 3583 anthony@histotechexchange.com > I know this is a problem that has plagued facilities for years and I > too have noticed a change in the past 2 years. Yes, the histology > programs nationwide produce a great albeit small group of talented > people every year but the pool of available histo techs for permanent > positions has shrunk even more in recent years. At the risk of being > "flamed" by travel companies I have to say that you are losing alot of > techs to travel positions. In the past 2 years of all of the histo > techs I have had contact with over half only want to work in permanent > positions the rest either want to continue as travelers or become > travelers. Think about it... they get a higher rate of pay, benefits > and living expenses paid for. For these people it is a "better deal" > than committing to one facility. As a matter of fact it is a "better > deal" than a temp/travel position in any other field outside of > healthcare. Facilities who take the "quick solution" of hiring travel > techs are contributing to the shortage. May I offer some solutions? Some creative hiring strategies? > > Here are some ideas I would like to share: > 1. If you are using travel techs do it with a temp to perm clause - > but be firm. If a tech works for you as a temp make sure they are at > least considering converting to a permanent employee at the end of the > contract. If not don't extend, have your travel company send someone > else who would consider converting to a permanent position. And make > questions about their intentions part of your interview process the > same as you would if you were interviewing a candidate from out of > state for a permanent position. > > 2. Human Resources - Many of your allied health recruiters don't seem > to realize that histo techs don't grow on trees. So many times I see > facilities lose great techs because the hiring process has dragged out > and the candidate ends up taking a position with a facility that can > move faster. Stay on top of your hr people especially once you know > they have a histology candidate. > > 3. How about techs from Canada? There are alot of talented techs in > Canada that are interested in moving to the states and the process is > relatively easy due to NAFTA and the F1 visa. > > 4. How about techs that need sponsorship on an H-1 visa? I know alot > of companies shy away from this alternative because of the length of > time it can take to process a visa application but I think that if you > take a look at the time it takes to find a tech at all against the > time it would take to process an H-1 visa it is quickly becoming 6 of one vs. > half dozen of another. I mean what difference does it make if it > takes up to 8 weeks to process an H-1 visa vs. 2-3 months to identify > a histology candidate? > > Your best bet is to get with your Human Resources department and > strategize, educate them on the challenges and shortages you are facing. > Discuss some of these options or others you might come up with. > I hope this helps!! > > Thank You! > > > Pam Barker > President > RELIA > Specialists in Allied Healthcare Recruiting > 5703 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell: (407)353-5070 > FAX: (407)678-2788 > E-mail: relia1@earthlink.net > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > *http://lists.utsouthwestern.edu/mailman/listinfo/histonet* > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu *http://lists.utsouthwestern.edu/mailman/listinfo/histonet* From gvdobbin <@t> ihis.org Thu Apr 10 07:37:03 2008 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Thu Apr 10 07:37:45 2008 Subject: [Histonet] Immunohistochemistry Machine Message-ID: Hello All, Regarding the "Wave" by Cellerus: This has to be referring to 15 mins after the slide has been dried, dewaxed, rehydrated and epitope retreived. 15 mins for actual detection time is great but not unheard of in the industry. And if I am correct in my assumption regarding the pre-detection steps, the "walk-away automation" is to be taken with a big grain of salt. If I am wrong, I'd love to hear the details. The websight is long on hype and short on details. Have a nice day folks. An old sceptic from way back... Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 There is some merit in doing the right thing rather badly, but absolutely none in doing the wrong thing excellently! >>> The Smith's 4/9/2008 4:16:02 PM >>> Ramona... I would additionally look into a new system from Celerus Diagnostics called 'The Wave'. A new continuous feed IHC stainer with 15 minute turn around time with walk away automation. It has an extremely small footprint as well...worth looking into if you are just starting your 'shopping'!! Visit www.celerusdiagnositc.com/wave Good Luck, Jackie Smith > From: mona_diane@hotmail.com> To: histonet@lists.utsouthwestern.edu> Date: Wed, 9 Apr 2008 12:20:45 -0400> Subject: [Histonet] Immunohistochemistry Machine> > > > > I am looking for a machine to process immunohistochemistry stains. I want something small, that will do as much online as possible with little hands on attention required. Any recommendations?> > Ramona> Potomac Hospital> _________________________________________________________________> Going green? See the top 12 foods to eat organic.> http://green.msn.com/galleries/photos/photos.aspx?gid=164&ocid=T003MSN51N1653A_______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Use video conversation to talk face-to-face with Windows Live Messenger. http://www.windowslive.com/messenger/connect_your_way.html?ocid=TXT_TAGLM_WL_Refresh_messenger_video_042008_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- From annigyg <@t> gmail.com Thu Apr 10 07:42:24 2008 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Thu Apr 10 07:42:27 2008 Subject: [Histonet] looking for ...... Message-ID: I'm looking for Jim Findlay from Glasgow Can anyone assist thanks Annie (now in Arabia) -- Anne (van Binsbergen) Hope Abu Dhabi UAE From awatanabe <@t> tgen.org Thu Apr 10 09:30:28 2008 From: awatanabe <@t> tgen.org (Aprill Watanabe) Date: Thu Apr 10 09:30:34 2008 Subject: [Histonet] Re: Immunohistochemistry machine In-Reply-To: <20080409170840.CB87B21C99F1@mr2.tgen.org> Message-ID: I would highly recommend the Bond from Leica. We are a research group and I use it for everything. It's got a relatively small footprint and bakes to counterstains on-line you only need to coverslip the slides. Aprill Watanabe, B.S. Research Associate Integrated Cancer Genomics Division Tissue Microarray Center (TMA) Translational Genomics Research Institute (TGen) main: 602-343-8822 Fax: 602-343-8840 awatanabe@tgen.org www.tgen.org From ploykasek <@t> phenopath.com Thu Apr 10 09:40:58 2008 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Thu Apr 10 09:41:16 2008 Subject: [Histonet] Histonet Vacancies In-Reply-To: <69272a080804091750h66cd0ddbj9abf11863885e45e@mail.gmail.com> Message-ID: Well said, Dan. I do have one small caveat, there is a new school in the Northwest, Tacoma WA area. It is at Clover Park Technical College. Check it out at www.cptc.edu. The area histotechs have been quite supportive, and I think the program is off to a great start. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > I'll add my two-cents worth. It's hard to believe that after 25 years in > this business that the histotech "shortage" situation is pretty much the > same as it was long ago. There have certainly been improvements in education > and training of people in histology, but for the most part (99%??) people > just fall into histology rather than pick it at some point in school. In > fact, it is a very rare day when I meet someone who went to school for > histotechnology, At this date there is still only one school on the west > coast, and it is not the same school that existed for a while in Seattle! > The new one is in southern california. I looked into starting a > histotechnology course here in the SF Bay Area, but it takes a long while > with lots of justification and scrounging for equipment and if I wanted to > do it full time the pay would be half as much as I make now. I could do it > part time but then I'd be working 60-70 hours a week rather than 50 I do > now. For the time being I give presentations to the local junior colleges. I > may expand that to high schools. I know they are interested in getting > speakers, but finding the time... > > I work in the biotech field as an IHC lab manger. We don't require > HT/HTL/QIHC (though would love to have them!)but it is still very difficult > to get experienced histotechs and nearly impossible to get experienced IHC > techs around here. Most of the people I interview are from the research > field who may have done some IHC at some point. And the ones who have good > IHC experience are making very high salaries that our company gags at > matching. It is not possible to recruit out of the Bay Area because the cost > of housing is so high that no one that is not already living here can afford > to move here. Even getting someone from southern cal is difficult - > justifiying relocation expenses is difficult. It is kind of a joke that the > experienced IHC techs know each other and spend their careers trading places > at the varioius companies. Anthony is quite right that it takes a lot of > effort to educate HR and upper management about the need for higher pay. > They really have a hard time justifying the same pay for a technologist that > a new PhD will also make. > > On top of that we have loads of (legal) immigrants from Asia here and they > are quite willing to work for lower pay to get a start. It is very common to > have PhD level people, and even immigrant MD's (who can't get board > certified here, mainly due to lanquage difficulties) working as lab techs. > Who is going to hire a AA/BS histotech when they can get a PhD who is happy > to do the work and able to contribute to R&D at a higher level than most > techs? > > There is a huge disparity between what hospitals and private diagnostic labs > pay. The local Kaiser labs and Univeristy hospital labs pay in the $35 per > hour range for basic histology. Senior IHC techs are in the $45 range. Those > places have unions which is why the pay is so high. On the other hand, > private labs will pay experieinced,certified people almost that much, but > new, inexperienced people may be paid $15-20/hr and the lab is not willing > to help them get to their HT/HTL because they don't want to pay them more, > and could lose them to the hospitals once trained. This is not speculation > but things I have had told to me first hand from techs who work in these > places. > > It is a little harder now for histotechs to be trained on the job due to the > AA requirement. Most OJT people I have met were working in the hospital at > some other job - lab assisant, dishwasher, etc before getting (accidently) > into histology. A person working on an AA is probably not working in those > jobs and probably does not even know a histology lab exists. > > Obviously the need is to get exposure for histotechnology. The NSH makes > brochures and videos available and does a career day for high schools > students at the national meeting every year (I've helped at those). This is > a good foundation. But it will really take every state society and every > interested histotech to do SOMETHING to promote the field. Science teachers > at high schools are very interested in having working people come in to talk > about a field. If someone in every city could do that it would go a long way > towards promoting the field. Maybe the key is to have NSH come up with a > standardised presentation that anyone can give along with some props to make > it a tactile experience. > > But, after the promotion you have to realize that it takes a huge amount of > initiative on the students part to follow through. They have to start work > on, or finish, an AA /BS degree, make contact with a lab that is interested > in training people, get the position, do on-the-job (maybe at the same time > as school) and THEN work on learning the matieral for an HT/HTL pretty much > alone. Their facing a 3 to 4 year process. Longer if they get a bachelors > degree. Key is finding labs to training that value HT/HTL certification. > Starting HT/HTL study groups would be a good way to get people motivated. > This is the kind of thing a state society can do. Individuals can to it as > well, but it helps to have a group for motivation. > > Of course, that is why it has not happened - it depends too much on a few > super-motivated individuals. The schools don't hear of a demand and so do > not have programs. No programs means students never hear about it. Since > students don't hear about it they can't even consider it. A vicious circle. > > Imagine if the schools had students asking about courses leading to > histotech certification or at least a chance at a job that leads to > certification. That gets the schools attention. Junior colleges especially > are very interested in providing trained people to the local job market. For > them jobs = program justification. > > Think about it. > > Dan in Danville (a pseudonom to allow free speech) > > > > > > > Dear Pam: > > For a long time histologists have gotten the short end of the stick. I > believe that the current situation is a symptom of the problem and is due to > a history of underpayment. > > We cannot solve the problem by artificial means. This country is based on > the free market system. We must work with the system not against it, but > first we must clearly understand the problem: Not enough techs, increasing > barriers to entry into the profession, lack of visibility, no central > planning to fix or even research the problem. > > We need to work to bring up the wages of HTs all across the country. If we > do this, we will bring Histology to the attention of people in education at > the moment. > > We can only get out of it by concentrating on increasing the number of > facilities that are prepared to take on new graduates and spend the time to > get their practical skills up to speed. > > I think you are doing a valuable job bringing this situation back to the > surface. We need to keep the momentum up and not let it die on the vine of > this valuable forum as a forgotten conversation. So, I ask my peers: What > can we do to address this situation in an organized, effective manner? Who > in our profession is responsible for being our cheerleader? > > Finally, Pam, I want to say for myself that I encourage all conversation. > > I will never be offended by a question, and I will always try to answer > questions in a way that remembers there is a person behind it. No flame > retardant needed here. > > I make my money from placing people on a temporary and permanent basis. > > I am also a Histologist first and foremost and believe that patient safety > is number one and understaffed laboratories experience more mistakes. > > Keep up the good work, Pam. > > All my best, > > Anthony Williams BSc. HT > > Histotech Exchange LLC > > 19 Whitmore St. > > Lexington, VA 24450 > > T 1 (302) 383 9780 > > F 1 (540) 463 3583 > > anthony@histotechexchange.com > >> I know this is a problem that has plagued facilities for years and I > >> too have noticed a change in the past 2 years. Yes, the histology > >> programs nationwide produce a great albeit small group of talented > >> people every year but the pool of available histo techs for permanent > >> positions has shrunk even more in recent years. At the risk of being > >> "flamed" by travel companies I have to say that you are losing alot of > >> techs to travel positions. In the past 2 years of all of the histo > >> techs I have had contact with over half only want to work in permanent > >> positions the rest either want to continue as travelers or become > >> travelers. Think about it... they get a higher rate of pay, benefits > >> and living expenses paid for. For these people it is a "better deal" > >> than committing to one facility. As a matter of fact it is a "better > >> deal" than a temp/travel position in any other field outside of > >> healthcare. Facilities who take the "quick solution" of hiring travel > >> techs are contributing to the shortage. May I offer some solutions? Some > creative hiring strategies? > >> > >> Here are some ideas I would like to share: > >> 1. If you are using travel techs do it with a temp to perm clause - > >> but be firm. If a tech works for you as a temp make sure they are at > >> least considering converting to a permanent employee at the end of the > >> contract. If not don't extend, have your travel company send someone > >> else who would consider converting to a permanent position. And make > >> questions about their intentions part of your interview process the > >> same as you would if you were interviewing a candidate from out of > >> state for a permanent position. > >> > >> 2. Human Resources - Many of your allied health recruiters don't seem > >> to realize that histo techs don't grow on trees. So many times I see > >> facilities lose great techs because the hiring process has dragged out > >> and the candidate ends up taking a position with a facility that can > >> move faster. Stay on top of your hr people especially once you know > >> they have a histology candidate. > >> > >> 3. How about techs from Canada? There are alot of talented techs in > >> Canada that are interested in moving to the states and the process is > >> relatively easy due to NAFTA and the F1 visa. > >> > >> 4. How about techs that need sponsorship on an H-1 visa? I know alot > >> of companies shy away from this alternative because of the length of > >> time it can take to process a visa application but I think that if you > >> take a look at the time it takes to find a tech at all against the > >> time it would take to process an H-1 visa it is quickly becoming 6 of one > vs. > >> half dozen of another. I mean what difference does it make if it > >> takes up to 8 weeks to process an H-1 visa vs. 2-3 months to identify > >> a histology candidate? > >> > >> Your best bet is to get with your Human Resources department and > >> strategize, educate them on the challenges and shortages you are facing. > >> Discuss some of these options or others you might come up with. > >> I hope this helps!! > >> > >> Thank You! > >> > >> > >> Pam Barker > >> President > >> RELIA > >> Specialists in Allied Healthcare Recruiting > >> 5703 Red Bug Lake Road #330 > >> Winter Springs, FL 32708-4969 > >> Phone: (407)657-2027 > >> Cell: (407)353-5070 > >> FAX: (407)678-2788 > >> E-mail: relia1@earthlink.net > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> > *http://lists.utsouthwestern.edu/mailman/listinfo/histonet* thwestern.edu/mailman/listinfo/histonet> > >> > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > *http://lists.utsouthwestern.edu/mailman/listinfo/histonet* thwestern.edu/mailman/listinfo/histonet> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From dellav <@t> musc.edu Thu Apr 10 09:44:54 2008 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Thu Apr 10 09:44:52 2008 Subject: [Histonet] antibodies to myosin for fast twitch and slow twitch striated muscle In-Reply-To: References: <20080409170840.CB87B21C99F1@mr2.tgen.org> Message-ID: Can someone recommend a source for antibodies to myosin that will differentiate fast and slow twitch fibers? Preferably products you use and are happy with. Thank you, Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Ph: (843) 792-6353 Fax: (843) 792-8974 From gmartin <@t> marshallmedical.org Thu Apr 10 10:07:45 2008 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Thu Apr 10 10:07:54 2008 Subject: [Histonet] Histo vacancies Message-ID: <6ED9D4252F278841A0593D3D788AF24C02246F81@mailsvr.MARSHMED.local> I have been reading these listing with great interest ... Dan's writing prompted this listing. I am one of those people who fell into histology because we did not have backup for our "certified" histo tech at our pathology lab. I do not have the "proper education" to qualify for a certification. The histo tech that I backup helped very little in my training, and shares a bit of the attitude I read on this listing sometimes, which is everybody should be certified or else they are not credible techs. I came in on my weekends and after hours to train myself on expired blocks, while putting myself through Freida Carson's self instruction text. I do agree that it is probably best to be officially trained, but with the shortage I believe that there should be some sort of variance to the rules (there may be ... but I haven't found it). I presently work with the local high schools in a program I helped developed called the Health Academy, which introduces students to different areas of health care via a shadowing program. The shadowing program and the presentations we give in the class rooms provide a great opportunity to introduce these student to histology. The question from these students is "where do you get trained for this ,how long does it take, and what are the wages" The answer to that question is usually not what they want to hear (exampled by these listings). One kid told me "if you get accepted to the plumbers training program through the union, you will start working on the job immediately, and be earning about thirty to forty thousand a year. Your requirements are, to attend school a couple of times a week (in the evenings) for four years. After completion you are a journeyman earning fifty to seventy thousand a year. Very hard to compete with that ! I don't know what the answer is, but it seems to me the restriction are getting tighter. I think there should be some levels of training allowed with an easier way to move forward to deeper training on the job. Thanks to all who have read and participated in this very interesting discussion Gary From richardwhorobin <@t> tomcroy.co.uk Thu Apr 10 10:19:52 2008 From: richardwhorobin <@t> tomcroy.co.uk (Richard) Date: Thu Apr 10 10:19:47 2008 Subject: [Histonet] Re: Nuclear Envelope/Membrane (Vernon Dailey) References: <47FCD987020000970001343C@n6mcgw16.cchmc.org> Message-ID: <000d01c89b1e$53560690$0200a8c0@delldim4500> Eric said, and I respond: >>> Use POPO3 Iodide, it is a nuclear membrane stain. This is in live intact cells is it? If so, have you a reference for this Eric, or indeed personal experience? I looked at the Invitrogen site for POPO3 [yes] and at their list of references and couldnt see anything plausible Do tell! Bye --- Richard Horobin Note: I'll be away at a conference, and hanging out with buddies, in Vermont from the 17th April to 6th May 2008. Academic: Div Neuroscience & Biomedical Systems, IBLS, University of Glasgow Home: Springbank, 20 Tomcroy Terrace, Pitlochry PH16 5JA, Scotland T 01796-474480 -- E RichardWHorobin@tomcroy.co.uk "One does not need to live a burden-driven life" From tim.morken <@t> thermofisher.com Thu Apr 10 10:36:55 2008 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Thu Apr 10 10:37:32 2008 Subject: [Histonet] Histonet Vacancies In-Reply-To: References: <69272a080804091750h66cd0ddbj9abf11863885e45e@mail.gmail.com> Message-ID: <6BFF6D137DF6BC43B33891BA96E83B190156C143@PGHCR-EXMB-VS-1.na.fshrnet.com> Patty, Thanks for the link to the new program. And the histology "DSI" game on the site is brilliant!! Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti Loykasek Sent: Thursday, April 10, 2008 7:41 AM To: Dan Dan; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Histonet Vacancies Well said, Dan. I do have one small caveat, there is a new school in the Northwest, Tacoma WA area. It is at Clover Park Technical College. Check it out at www.cptc.edu. The area histotechs have been quite supportive, and I think the program is off to a great start. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > I'll add my two-cents worth. It's hard to believe that after 25 years > in this business that the histotech "shortage" situation is pretty > much the same as it was long ago. There have certainly been > improvements in education and training of people in histology, but for > the most part (99%??) people just fall into histology rather than pick > it at some point in school. In fact, it is a very rare day when I meet > someone who went to school for histotechnology, At this date there is > still only one school on the west coast, and it is not the same school that existed for a while in Seattle! > The new one is in southern california. I looked into starting a > histotechnology course here in the SF Bay Area, but it takes a long > while with lots of justification and scrounging for equipment and if I > wanted to do it full time the pay would be half as much as I make now. > I could do it part time but then I'd be working 60-70 hours a week > rather than 50 I do now. For the time being I give presentations to > the local junior colleges. I may expand that to high schools. I know > they are interested in getting speakers, but finding the time... > > I work in the biotech field as an IHC lab manger. We don't require > HT/HTL/QIHC (though would love to have them!)but it is still very > difficult to get experienced histotechs and nearly impossible to get > experienced IHC techs around here. Most of the people I interview are > from the research field who may have done some IHC at some point. And > the ones who have good IHC experience are making very high salaries > that our company gags at matching. It is not possible to recruit out > of the Bay Area because the cost of housing is so high that no one > that is not already living here can afford to move here. Even getting > someone from southern cal is difficult - justifiying relocation > expenses is difficult. It is kind of a joke that the experienced IHC > techs know each other and spend their careers trading places at the > varioius companies. Anthony is quite right that it takes a lot of effort to educate HR and upper management about the need for higher pay. > They really have a hard time justifying the same pay for a > technologist that a new PhD will also make. > > On top of that we have loads of (legal) immigrants from Asia here and > they are quite willing to work for lower pay to get a start. It is > very common to have PhD level people, and even immigrant MD's (who > can't get board certified here, mainly due to lanquage difficulties) working as lab techs. > Who is going to hire a AA/BS histotech when they can get a PhD who is > happy to do the work and able to contribute to R&D at a higher level > than most techs? > > There is a huge disparity between what hospitals and private > diagnostic labs pay. The local Kaiser labs and Univeristy hospital > labs pay in the $35 per hour range for basic histology. Senior IHC > techs are in the $45 range. Those places have unions which is why the > pay is so high. On the other hand, private labs will pay > experieinced,certified people almost that much, but new, inexperienced > people may be paid $15-20/hr and the lab is not willing to help them > get to their HT/HTL because they don't want to pay them more, and > could lose them to the hospitals once trained. This is not speculation > but things I have had told to me first hand from techs who work in these places. > > It is a little harder now for histotechs to be trained on the job due > to the AA requirement. Most OJT people I have met were working in the > hospital at some other job - lab assisant, dishwasher, etc before > getting (accidently) into histology. A person working on an AA is > probably not working in those jobs and probably does not even know a histology lab exists. > > Obviously the need is to get exposure for histotechnology. The NSH > makes brochures and videos available and does a career day for high > schools students at the national meeting every year (I've helped at > those). This is a good foundation. But it will really take every state > society and every interested histotech to do SOMETHING to promote the > field. Science teachers at high schools are very interested in having > working people come in to talk about a field. If someone in every city > could do that it would go a long way towards promoting the field. > Maybe the key is to have NSH come up with a standardised presentation > that anyone can give along with some props to make it a tactile experience. > > But, after the promotion you have to realize that it takes a huge > amount of initiative on the students part to follow through. They have > to start work on, or finish, an AA /BS degree, make contact with a lab > that is interested in training people, get the position, do on-the-job > (maybe at the same time as school) and THEN work on learning the > matieral for an HT/HTL pretty much alone. Their facing a 3 to 4 year > process. Longer if they get a bachelors degree. Key is finding labs to training that value HT/HTL certification. > Starting HT/HTL study groups would be a good way to get people motivated. > This is the kind of thing a state society can do. Individuals can to > it as well, but it helps to have a group for motivation. > > Of course, that is why it has not happened - it depends too much on a > few super-motivated individuals. The schools don't hear of a demand > and so do not have programs. No programs means students never hear > about it. Since students don't hear about it they can't even consider it. A vicious circle. > > Imagine if the schools had students asking about courses leading to > histotech certification or at least a chance at a job that leads to > certification. That gets the schools attention. Junior colleges > especially are very interested in providing trained people to the > local job market. For them jobs = program justification. > > Think about it. > > Dan in Danville (a pseudonom to allow free speech) > > > > > > > Dear Pam: > > For a long time histologists have gotten the short end of the stick. I > believe that the current situation is a symptom of the problem and is > due to a history of underpayment. > > We cannot solve the problem by artificial means. This country is based > on the free market system. We must work with the system not against > it, but first we must clearly understand the problem: Not enough > techs, increasing barriers to entry into the profession, lack of > visibility, no central planning to fix or even research the problem. > > We need to work to bring up the wages of HTs all across the country. > If we do this, we will bring Histology to the attention of people in > education at the moment. > > We can only get out of it by concentrating on increasing the number of > facilities that are prepared to take on new graduates and spend the > time to get their practical skills up to speed. > > I think you are doing a valuable job bringing this situation back to > the surface. We need to keep the momentum up and not let it die on the > vine of this valuable forum as a forgotten conversation. So, I ask my > peers: What can we do to address this situation in an organized, > effective manner? Who in our profession is responsible for being our cheerleader? > > Finally, Pam, I want to say for myself that I encourage all conversation. > > I will never be offended by a question, and I will always try to > answer questions in a way that remembers there is a person behind it. > No flame retardant needed here. > > I make my money from placing people on a temporary and permanent basis. > > I am also a Histologist first and foremost and believe that patient > safety is number one and understaffed laboratories experience more mistakes. > > Keep up the good work, Pam. > > All my best, > > Anthony Williams BSc. HT > > Histotech Exchange LLC > > 19 Whitmore St. > > Lexington, VA 24450 > > T 1 (302) 383 9780 > > F 1 (540) 463 3583 > > anthony@histotechexchange.com > >> I know this is a problem that has plagued facilities for years and I > >> too have noticed a change in the past 2 years. Yes, the histology > >> programs nationwide produce a great albeit small group of talented > >> people every year but the pool of available histo techs for permanent > >> positions has shrunk even more in recent years. At the risk of being > >> "flamed" by travel companies I have to say that you are losing alot >> of > >> techs to travel positions. In the past 2 years of all of the histo > >> techs I have had contact with over half only want to work in >> permanent > >> positions the rest either want to continue as travelers or become > >> travelers. Think about it... they get a higher rate of pay, benefits > >> and living expenses paid for. For these people it is a "better deal" > >> than committing to one facility. As a matter of fact it is a "better > >> deal" than a temp/travel position in any other field outside of > >> healthcare. Facilities who take the "quick solution" of hiring travel > >> techs are contributing to the shortage. May I offer some solutions? >> Some > creative hiring strategies? > >> > >> Here are some ideas I would like to share: > >> 1. If you are using travel techs do it with a temp to perm clause - > >> but be firm. If a tech works for you as a temp make sure they are at > >> least considering converting to a permanent employee at the end of >> the > >> contract. If not don't extend, have your travel company send someone > >> else who would consider converting to a permanent position. And make > >> questions about their intentions part of your interview process the > >> same as you would if you were interviewing a candidate from out of > >> state for a permanent position. > >> > >> 2. Human Resources - Many of your allied health recruiters don't seem > >> to realize that histo techs don't grow on trees. So many times I see > >> facilities lose great techs because the hiring process has dragged >> out > >> and the candidate ends up taking a position with a facility that can > >> move faster. Stay on top of your hr people especially once you know > >> they have a histology candidate. > >> > >> 3. How about techs from Canada? There are alot of talented techs in > >> Canada that are interested in moving to the states and the process is > >> relatively easy due to NAFTA and the F1 visa. > >> > >> 4. How about techs that need sponsorship on an H-1 visa? I know alot > >> of companies shy away from this alternative because of the length of > >> time it can take to process a visa application but I think that if >> you > >> take a look at the time it takes to find a tech at all against the > >> time it would take to process an H-1 visa it is quickly becoming 6 of >> one > vs. > >> half dozen of another. I mean what difference does it make if it > >> takes up to 8 weeks to process an H-1 visa vs. 2-3 months to identify > >> a histology candidate? > >> > >> Your best bet is to get with your Human Resources department and > >> strategize, educate them on the challenges and shortages you are facing. > >> Discuss some of these options or others you might come up with. > >> I hope this helps!! > >> > >> Thank You! > >> > >> > >> Pam Barker > >> President > >> RELIA > >> Specialists in Allied Healthcare Recruiting > >> 5703 Red Bug Lake Road #330 > >> Winter Springs, FL 32708-4969 > >> Phone: (407)657-2027 > >> Cell: (407)353-5070 > >> FAX: (407)678-2788 > >> E-mail: relia1@earthlink.net > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> > *http://lists.utsouthwestern.edu/mailman/listinfo/histonet* ts.utsou thwestern.edu/mailman/listinfo/histonet> > >> > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > *http://lists.utsouthwestern.edu/mailman/listinfo/histonet* ts.utsou thwestern.edu/mailman/listinfo/histonet> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kappeler <@t> patho.unibe.ch Thu Apr 10 11:20:50 2008 From: kappeler <@t> patho.unibe.ch (Andi Kappeler) Date: Thu Apr 10 11:21:11 2008 Subject: AW: [Histonet] antibodies to myosin for fast twitch and slow twitchstriated muscle In-Reply-To: References: <20080409170840.CB87B21C99F1@mr2.tgen.org> Message-ID: <003a01c89b26$d788abe0$17955c82@pi23> Hi Vinnie We routinely use the following mouse monoclonal antibodies on FFPE human skeletal muscle: Myosin I (slow), clone NOQ7.5.4D, Sigma M-8421; working conc. 5 ug Ig/ml, HIER in 10 mM citrate buffer, pH 6.0, pressure cooker. Myosin II (fast), clone MY-31, Sigma M.4276; working conc. 10 ug Ig/ml, HIER as above. Hope this helps. Andi Kappeler Insitute of Pathology, University of Bern, Switzerland -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Della Speranza, Vinnie Gesendet: Donnerstag, 10. April 2008 16:45 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] antibodies to myosin for fast twitch and slow twitchstriated muscle Can someone recommend a source for antibodies to myosin that will differentiate fast and slow twitch fibers? Preferably products you use and are happy with. Thank you, Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Ph: (843) 792-6353 Fax: (843) 792-8974 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From integrated.histo <@t> gmail.com Thu Apr 10 12:12:54 2008 From: integrated.histo <@t> gmail.com (Cindy DuBois) Date: Thu Apr 10 12:13:19 2008 Subject: [Histonet] Salary / Temp positions Message-ID: <5d9104a30804101012y48035d2cwefc3148107838ed9@mail.gmail.com> If we think about the cost of 2-4 years of college to obtain a degree in order to qualify for the Histology test, most of us would be in debt when finished. Then look at our salary and you can see how the requirements just aren't supported by the salary. Most of the students will come out of college owing on student loans. The salaries they would receive as new histotech would allow them to pay off their student loanswhile maintaining a decent living (at least here in CA). With both my sons in college (using student loans) we had to take a serious look at the final amount they will owe when the graduate and compare it to what they would be earning. I am not sure what the solution is. Any ideas? Cindy From cmiller <@t> physlab.com Thu Apr 10 12:18:18 2008 From: cmiller <@t> physlab.com (Cheri Miller) Date: Thu Apr 10 12:18:00 2008 Subject: [Histonet] Histonet Vacancies In-Reply-To: <6BFF6D137DF6BC43B33891BA96E83B190156C143@PGHCR-EXMB-VS-1.na.fshrnet.com> References: <69272a080804091750h66cd0ddbj9abf11863885e45e@mail.gmail.com> <6BFF6D137DF6BC43B33891BA96E83B190156C143@PGHCR-EXMB-VS-1.na.fshrnet.com> Message-ID: <000301c89b2e$dec2fa20$3d02a8c0@plab.local> I agree, its very clever! I have saved it to share with my department! Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Thursday, April 10, 2008 10:37 AM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histonet Vacancies Patty, Thanks for the link to the new program. And the histology "DSI" game on the site is brilliant!! Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti Loykasek Sent: Thursday, April 10, 2008 7:41 AM To: Dan Dan; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Histonet Vacancies Well said, Dan. I do have one small caveat, there is a new school in the Northwest, Tacoma WA area. It is at Clover Park Technical College. Check it out at www.cptc.edu. The area histotechs have been quite supportive, and I think the program is off to a great start. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > I'll add my two-cents worth. It's hard to believe that after 25 years > in this business that the histotech "shortage" situation is pretty > much the same as it was long ago. There have certainly been > improvements in education and training of people in histology, but for > the most part (99%??) people just fall into histology rather than pick > it at some point in school. In fact, it is a very rare day when I meet > someone who went to school for histotechnology, At this date there is > still only one school on the west coast, and it is not the same school that existed for a while in Seattle! > The new one is in southern california. I looked into starting a > histotechnology course here in the SF Bay Area, but it takes a long > while with lots of justification and scrounging for equipment and if I > wanted to do it full time the pay would be half as much as I make now. > I could do it part time but then I'd be working 60-70 hours a week > rather than 50 I do now. For the time being I give presentations to > the local junior colleges. I may expand that to high schools. I know > they are interested in getting speakers, but finding the time... > > I work in the biotech field as an IHC lab manger. We don't require > HT/HTL/QIHC (though would love to have them!)but it is still very > difficult to get experienced histotechs and nearly impossible to get > experienced IHC techs around here. Most of the people I interview are > from the research field who may have done some IHC at some point. And > the ones who have good IHC experience are making very high salaries > that our company gags at matching. It is not possible to recruit out > of the Bay Area because the cost of housing is so high that no one > that is not already living here can afford to move here. Even getting > someone from southern cal is difficult - justifiying relocation > expenses is difficult. It is kind of a joke that the experienced IHC > techs know each other and spend their careers trading places at the > varioius companies. Anthony is quite right that it takes a lot of effort to educate HR and upper management about the need for higher pay. > They really have a hard time justifying the same pay for a > technologist that a new PhD will also make. > > On top of that we have loads of (legal) immigrants from Asia here and > they are quite willing to work for lower pay to get a start. It is > very common to have PhD level people, and even immigrant MD's (who > can't get board certified here, mainly due to lanquage difficulties) working as lab techs. > Who is going to hire a AA/BS histotech when they can get a PhD who is > happy to do the work and able to contribute to R&D at a higher level > than most techs? > > There is a huge disparity between what hospitals and private > diagnostic labs pay. The local Kaiser labs and Univeristy hospital > labs pay in the $35 per hour range for basic histology. Senior IHC > techs are in the $45 range. Those places have unions which is why the > pay is so high. On the other hand, private labs will pay > experieinced,certified people almost that much, but new, inexperienced > people may be paid $15-20/hr and the lab is not willing to help them > get to their HT/HTL because they don't want to pay them more, and > could lose them to the hospitals once trained. This is not speculation > but things I have had told to me first hand from techs who work in these places. > > It is a little harder now for histotechs to be trained on the job due > to the AA requirement. Most OJT people I have met were working in the > hospital at some other job - lab assisant, dishwasher, etc before > getting (accidently) into histology. A person working on an AA is > probably not working in those jobs and probably does not even know a histology lab exists. > > Obviously the need is to get exposure for histotechnology. The NSH > makes brochures and videos available and does a career day for high > schools students at the national meeting every year (I've helped at > those). This is a good foundation. But it will really take every state > society and every interested histotech to do SOMETHING to promote the > field. Science teachers at high schools are very interested in having > working people come in to talk about a field. If someone in every city > could do that it would go a long way towards promoting the field. > Maybe the key is to have NSH come up with a standardised presentation > that anyone can give along with some props to make it a tactile experience. > > But, after the promotion you have to realize that it takes a huge > amount of initiative on the students part to follow through. They have > to start work on, or finish, an AA /BS degree, make contact with a lab > that is interested in training people, get the position, do on-the-job > (maybe at the same time as school) and THEN work on learning the > matieral for an HT/HTL pretty much alone. Their facing a 3 to 4 year > process. Longer if they get a bachelors degree. Key is finding labs to training that value HT/HTL certification. > Starting HT/HTL study groups would be a good way to get people motivated. > This is the kind of thing a state society can do. Individuals can to > it as well, but it helps to have a group for motivation. > > Of course, that is why it has not happened - it depends too much on a > few super-motivated individuals. The schools don't hear of a demand > and so do not have programs. No programs means students never hear > about it. Since students don't hear about it they can't even consider it. A vicious circle. > > Imagine if the schools had students asking about courses leading to > histotech certification or at least a chance at a job that leads to > certification. That gets the schools attention. Junior colleges > especially are very interested in providing trained people to the > local job market. For them jobs = program justification. > > Think about it. > > Dan in Danville (a pseudonom to allow free speech) > > > > > > > Dear Pam: > > For a long time histologists have gotten the short end of the stick. I > believe that the current situation is a symptom of the problem and is > due to a history of underpayment. > > We cannot solve the problem by artificial means. This country is based > on the free market system. We must work with the system not against > it, but first we must clearly understand the problem: Not enough > techs, increasing barriers to entry into the profession, lack of > visibility, no central planning to fix or even research the problem. > > We need to work to bring up the wages of HTs all across the country. > If we do this, we will bring Histology to the attention of people in > education at the moment. > > We can only get out of it by concentrating on increasing the number of > facilities that are prepared to take on new graduates and spend the > time to get their practical skills up to speed. > > I think you are doing a valuable job bringing this situation back to > the surface. We need to keep the momentum up and not let it die on the > vine of this valuable forum as a forgotten conversation. So, I ask my > peers: What can we do to address this situation in an organized, > effective manner? Who in our profession is responsible for being our cheerleader? > > Finally, Pam, I want to say for myself that I encourage all conversation. > > I will never be offended by a question, and I will always try to > answer questions in a way that remembers there is a person behind it. > No flame retardant needed here. > > I make my money from placing people on a temporary and permanent basis. > > I am also a Histologist first and foremost and believe that patient > safety is number one and understaffed laboratories experience more mistakes. > > Keep up the good work, Pam. > > All my best, > > Anthony Williams BSc. HT > > Histotech Exchange LLC > > 19 Whitmore St. > > Lexington, VA 24450 > > T 1 (302) 383 9780 > > F 1 (540) 463 3583 > > anthony@histotechexchange.com > >> I know this is a problem that has plagued facilities for years and I > >> too have noticed a change in the past 2 years. Yes, the histology > >> programs nationwide produce a great albeit small group of talented > >> people every year but the pool of available histo techs for permanent > >> positions has shrunk even more in recent years. At the risk of being > >> "flamed" by travel companies I have to say that you are losing alot >> of > >> techs to travel positions. In the past 2 years of all of the histo > >> techs I have had contact with over half only want to work in >> permanent > >> positions the rest either want to continue as travelers or become > >> travelers. Think about it... they get a higher rate of pay, benefits > >> and living expenses paid for. For these people it is a "better deal" > >> than committing to one facility. As a matter of fact it is a "better > >> deal" than a temp/travel position in any other field outside of > >> healthcare. Facilities who take the "quick solution" of hiring travel > >> techs are contributing to the shortage. May I offer some solutions? >> Some > creative hiring strategies? > >> > >> Here are some ideas I would like to share: > >> 1. If you are using travel techs do it with a temp to perm clause - > >> but be firm. If a tech works for you as a temp make sure they are at > >> least considering converting to a permanent employee at the end of >> the > >> contract. If not don't extend, have your travel company send someone > >> else who would consider converting to a permanent position. And make > >> questions about their intentions part of your interview process the > >> same as you would if you were interviewing a candidate from out of > >> state for a permanent position. > >> > >> 2. Human Resources - Many of your allied health recruiters don't seem > >> to realize that histo techs don't grow on trees. So many times I see > >> facilities lose great techs because the hiring process has dragged >> out > >> and the candidate ends up taking a position with a facility that can > >> move faster. Stay on top of your hr people especially once you know > >> they have a histology candidate. > >> > >> 3. How about techs from Canada? There are alot of talented techs in > >> Canada that are interested in moving to the states and the process is > >> relatively easy due to NAFTA and the F1 visa. > >> > >> 4. How about techs that need sponsorship on an H-1 visa? I know alot > >> of companies shy away from this alternative because of the length of > >> time it can take to process a visa application but I think that if >> you > >> take a look at the time it takes to find a tech at all against the > >> time it would take to process an H-1 visa it is quickly becoming 6 of >> one > vs. > >> half dozen of another. I mean what difference does it make if it > >> takes up to 8 weeks to process an H-1 visa vs. 2-3 months to identify > >> a histology candidate? > >> > >> Your best bet is to get with your Human Resources department and > >> strategize, educate them on the challenges and shortages you are facing. > >> Discuss some of these options or others you might come up with. > >> I hope this helps!! > >> > >> Thank You! > >> > >> > >> Pam Barker > >> President > >> RELIA > >> Specialists in Allied Healthcare Recruiting > >> 5703 Red Bug Lake Road #330 > >> Winter Springs, FL 32708-4969 > >> Phone: (407)657-2027 > >> Cell: (407)353-5070 > >> FAX: (407)678-2788 > >> E-mail: relia1@earthlink.net > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> > *http://lists.utsouthwestern.edu/mailman/listinfo/histonet* ts.utsou thwestern.edu/mailman/listinfo/histonet> > >> > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > *http://lists.utsouthwestern.edu/mailman/listinfo/histonet* ts.utsou thwestern.edu/mailman/listinfo/histonet> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From pkromund <@t> gundluth.org Thu Apr 10 12:18:35 2008 From: pkromund <@t> gundluth.org (pkromund@gundluth.org) Date: Thu Apr 10 12:18:43 2008 Subject: [Histonet] Salary / Temp positions In-Reply-To: <5d9104a30804101012y48035d2cwefc3148107838ed9@mail.gmail.com> Message-ID: Are Histology Lab attempting or being successful at getting their pay scales upgraded? Thanks, Pam "Cindy DuBois" To Sent by: histonet@lists.utsouthwestern.edu histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] Salary / Temp positions 04/10/2008 12:12 PM If we think about the cost of 2-4 years of college to obtain a degree in order to qualify for the Histology test, most of us would be in debt when finished. Then look at our salary and you can see how the requirements just aren't supported by the salary. Most of the students will come out of college owing on student loans. The salaries they would receive as new histotech would allow them to pay off their student loanswhile maintaining a decent living (at least here in CA). With both my sons in college (using student loans) we had to take a serious look at the final amount they will owe when the graduate and compare it to what they would be earning. I am not sure what the solution is. Any ideas? Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From asmith <@t> mail.barry.edu Thu Apr 10 12:20:57 2008 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Thu Apr 10 12:21:02 2008 Subject: [Histonet] histotech vacancies Message-ID: Finding a first-rate tech can be a real problem. Once you have a good one, all you have to do is to pay well. My mentor, William Montagna, kept his great tech for 20 years by calling him a research associate and paying accordingly. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Podiatric Medicine Miami Shores, Florida From JCollins <@t> palmbeachpath.com Thu Apr 10 12:37:24 2008 From: JCollins <@t> palmbeachpath.com (Judy Collins) Date: Thu Apr 10 12:37:29 2008 Subject: [Histonet] Salary / Temp positions In-Reply-To: <5d9104a30804101012y48035d2cwefc3148107838ed9@mail.gmail.com> References: <5d9104a30804101012y48035d2cwefc3148107838ed9@mail.gmail.com> Message-ID: <05CAE76AB5D5ED409864C6DD86F13349018863FF64@pbpsflexch02.pbp.local> What about the nontraditional learning programs such as Indiana University's? Our laboratory has helped 5 prospective techs through the past few years to become histotechnicians through their program. The program is 10 months long at a very reasonable cost and the candidates are eligible to sit for the ASCP at the end of the program. I believe there are some other similar programs as well. Judy Collins Palm Beach Pathology -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy DuBois Sent: Thursday, April 10, 2008 1:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Salary / Temp positions If we think about the cost of 2-4 years of college to obtain a degree in order to qualify for the Histology test, most of us would be in debt when finished. Then look at our salary and you can see how the requirements just aren't supported by the salary. Most of the students will come out of college owing on student loans. The salaries they would receive as new histotech would allow them to pay off their student loanswhile maintaining a decent living (at least here in CA). With both my sons in college (using student loans) we had to take a serious look at the final amount they will owe when the graduate and compare it to what they would be earning. I am not sure what the solution is. Any ideas? Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From juditw <@t> u.washington.edu Thu Apr 10 12:41:12 2008 From: juditw <@t> u.washington.edu (Judith L. Williams) Date: Thu Apr 10 12:41:19 2008 Subject: [Histonet] Histonet Vacancies---DSI !! In-Reply-To: <000301c89b2e$dec2fa20$3d02a8c0@plab.local> Message-ID: HI - I am Judith Williams, the creator of DSI!!! along with Jason Kruse. I was the instructor at Clover Park Tech College last year and then I took another job at Univ of WAshington. I designed the idea and text along with Jason, one of their computer experts at the college. He did a fantastic job of making it interesting! He came to the lab and took photos and I explained each step- he took notes then made the cartoons and applied it. He and I worked on it, editing and tweeking it - he is so clever! I really want him to get a lot of credit for it. Isn't it wonderful! He and I are going to make CD's of it to send out for advertising histotechnology. Will let you know about when they are going to be ready. Glad you all enjoyed it. I am a huge CSI fan - so naturally we did that twist! cheers to all histoworld!!! We need to sell this career - its great! Judith Williams, PhD, HT(ASCP) Department of Comparative Medicine University of Washington Seattle, WA On Thu, 10 Apr 2008, Cheri Miller wrote: > I agree, its very clever! I have saved it to share with my department! > Cheryl Miller HT (ASCP) > Histology Supervisor > Physicians Laboratory,P.C. > Omaha, Ne. > 402 738 5052 > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim > Sent: Thursday, April 10, 2008 10:37 AM > To: Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Histonet Vacancies > > > Patty, Thanks for the link to the new program. And the histology "DSI" > game on the site is brilliant!! > > > Tim Morken > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti > Loykasek > Sent: Thursday, April 10, 2008 7:41 AM > To: Dan Dan; Histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Histonet Vacancies > > Well said, Dan. I do have one small caveat, there is a new school in the > Northwest, Tacoma WA area. It is at Clover Park Technical College. Check > it out at www.cptc.edu. The area histotechs have been quite supportive, > and I think the program is off to a great start. > > > Patti Loykasek BS, HTL, QIHC > PhenoPath Laboratories > Seattle, WA > > > > >> I'll add my two-cents worth. It's hard to believe that after 25 years >> in this business that the histotech "shortage" situation is pretty >> much the same as it was long ago. There have certainly been >> improvements in education and training of people in histology, but for > >> the most part (99%??) people just fall into histology rather than pick > >> it at some point in school. In fact, it is a very rare day when I meet > >> someone who went to school for histotechnology, At this date there is >> still only one school on the west coast, and it is not the same school > that existed for a while in Seattle! >> The new one is in southern california. I looked into starting a >> histotechnology course here in the SF Bay Area, but it takes a long >> while with lots of justification and scrounging for equipment and if I > >> wanted to do it full time the pay would be half as much as I make now. > >> I could do it part time but then I'd be working 60-70 hours a week >> rather than 50 I do now. For the time being I give presentations to >> the local junior colleges. I may expand that to high schools. I know >> they are interested in getting speakers, but finding the time... >> >> I work in the biotech field as an IHC lab manger. We don't require >> HT/HTL/QIHC (though would love to have them!)but it is still very >> difficult to get experienced histotechs and nearly impossible to get >> experienced IHC techs around here. Most of the people I interview are >> from the research field who may have done some IHC at some point. And >> the ones who have good IHC experience are making very high salaries >> that our company gags at matching. It is not possible to recruit out >> of the Bay Area because the cost of housing is so high that no one >> that is not already living here can afford to move here. Even getting >> someone from southern cal is difficult - justifiying relocation >> expenses is difficult. It is kind of a joke that the experienced IHC >> techs know each other and spend their careers trading places at the >> varioius companies. Anthony is quite right that it takes a lot of > effort to educate HR and upper management about the need for higher pay. >> They really have a hard time justifying the same pay for a >> technologist that a new PhD will also make. >> >> On top of that we have loads of (legal) immigrants from Asia here and >> they are quite willing to work for lower pay to get a start. It is >> very common to have PhD level people, and even immigrant MD's (who >> can't get board certified here, mainly due to lanquage difficulties) > working as lab techs. >> Who is going to hire a AA/BS histotech when they can get a PhD who is >> happy to do the work and able to contribute to R&D at a higher level >> than most techs? >> >> There is a huge disparity between what hospitals and private >> diagnostic labs pay. The local Kaiser labs and Univeristy hospital >> labs pay in the $35 per hour range for basic histology. Senior IHC >> techs are in the $45 range. Those places have unions which is why the >> pay is so high. On the other hand, private labs will pay >> experieinced,certified people almost that much, but new, inexperienced > >> people may be paid $15-20/hr and the lab is not willing to help them >> get to their HT/HTL because they don't want to pay them more, and >> could lose them to the hospitals once trained. This is not speculation > >> but things I have had told to me first hand from techs who work in > these places. >> >> It is a little harder now for histotechs to be trained on the job due >> to the AA requirement. Most OJT people I have met were working in the >> hospital at some other job - lab assisant, dishwasher, etc before >> getting (accidently) into histology. A person working on an AA is >> probably not working in those jobs and probably does not even know a > histology lab exists. >> >> Obviously the need is to get exposure for histotechnology. The NSH >> makes brochures and videos available and does a career day for high >> schools students at the national meeting every year (I've helped at >> those). This is a good foundation. But it will really take every state > >> society and every interested histotech to do SOMETHING to promote the >> field. Science teachers at high schools are very interested in having >> working people come in to talk about a field. If someone in every city > >> could do that it would go a long way towards promoting the field. >> Maybe the key is to have NSH come up with a standardised presentation >> that anyone can give along with some props to make it a tactile > experience. >> >> But, after the promotion you have to realize that it takes a huge >> amount of initiative on the students part to follow through. They have > >> to start work on, or finish, an AA /BS degree, make contact with a lab > >> that is interested in training people, get the position, do on-the-job > >> (maybe at the same time as school) and THEN work on learning the >> matieral for an HT/HTL pretty much alone. Their facing a 3 to 4 year >> process. Longer if they get a bachelors degree. Key is finding labs to > training that value HT/HTL certification. >> Starting HT/HTL study groups would be a good way to get people > motivated. >> This is the kind of thing a state society can do. Individuals can to >> it as well, but it helps to have a group for motivation. >> >> Of course, that is why it has not happened - it depends too much on a >> few super-motivated individuals. The schools don't hear of a demand >> and so do not have programs. No programs means students never hear >> about it. Since students don't hear about it they can't even consider > it. A vicious circle. >> >> Imagine if the schools had students asking about courses leading to >> histotech certification or at least a chance at a job that leads to >> certification. That gets the schools attention. Junior colleges >> especially are very interested in providing trained people to the >> local job market. For them jobs = program justification. >> >> Think about it. >> >> Dan in Danville (a pseudonom to allow free speech) >> >> >> >> >> >> >> Dear Pam: >> >> For a long time histologists have gotten the short end of the stick. I > >> believe that the current situation is a symptom of the problem and is >> due to a history of underpayment. >> >> We cannot solve the problem by artificial means. This country is based > >> on the free market system. We must work with the system not against >> it, but first we must clearly understand the problem: Not enough >> techs, increasing barriers to entry into the profession, lack of >> visibility, no central planning to fix or even research the problem. >> >> We need to work to bring up the wages of HTs all across the country. >> If we do this, we will bring Histology to the attention of people in >> education at the moment. >> >> We can only get out of it by concentrating on increasing the number of > >> facilities that are prepared to take on new graduates and spend the >> time to get their practical skills up to speed. >> >> I think you are doing a valuable job bringing this situation back to >> the surface. We need to keep the momentum up and not let it die on the > >> vine of this valuable forum as a forgotten conversation. So, I ask my >> peers: What can we do to address this situation in an organized, >> effective manner? Who in our profession is responsible for being our > cheerleader? >> >> Finally, Pam, I want to say for myself that I encourage all > conversation. >> >> I will never be offended by a question, and I will always try to >> answer questions in a way that remembers there is a person behind it. >> No flame retardant needed here. >> >> I make my money from placing people on a temporary and permanent > basis. >> >> I am also a Histologist first and foremost and believe that patient >> safety is number one and understaffed laboratories experience more > mistakes. >> >> Keep up the good work, Pam. >> >> All my best, >> >> Anthony Williams BSc. HT >> >> Histotech Exchange LLC >> >> 19 Whitmore St. >> >> Lexington, VA 24450 >> >> T 1 (302) 383 9780 >> >> F 1 (540) 463 3583 >> >> anthony@histotechexchange.com >> >>> I know this is a problem that has plagued facilities for years and I >> >>> too have noticed a change in the past 2 years. Yes, the histology >> >>> programs nationwide produce a great albeit small group of talented >> >>> people every year but the pool of available histo techs for permanent >> >>> positions has shrunk even more in recent years. At the risk of being >> >>> "flamed" by travel companies I have to say that you are losing alot >>> of >> >>> techs to travel positions. In the past 2 years of all of the histo >> >>> techs I have had contact with over half only want to work in >>> permanent >> >>> positions the rest either want to continue as travelers or become >> >>> travelers. Think about it... they get a higher rate of pay, benefits >> >>> and living expenses paid for. For these people it is a "better deal" >> >>> than committing to one facility. As a matter of fact it is a "better >> >>> deal" than a temp/travel position in any other field outside of >> >>> healthcare. Facilities who take the "quick solution" of hiring travel >> >>> techs are contributing to the shortage. May I offer some solutions? >>> Some >> creative hiring strategies? >> >>> >> >>> Here are some ideas I would like to share: >> >>> 1. If you are using travel techs do it with a temp to perm clause - >> >>> but be firm. If a tech works for you as a temp make sure they are at >> >>> least considering converting to a permanent employee at the end of >>> the >> >>> contract. If not don't extend, have your travel company send someone >> >>> else who would consider converting to a permanent position. And make >> >>> questions about their intentions part of your interview process the >> >>> same as you would if you were interviewing a candidate from out of >> >>> state for a permanent position. >> >>> >> >>> 2. Human Resources - Many of your allied health recruiters don't seem >> >>> to realize that histo techs don't grow on trees. So many times I see >> >>> facilities lose great techs because the hiring process has dragged >>> out >> >>> and the candidate ends up taking a position with a facility that can >> >>> move faster. Stay on top of your hr people especially once you know >> >>> they have a histology candidate. >> >>> >> >>> 3. How about techs from Canada? There are alot of talented techs in >> >>> Canada that are interested in moving to the states and the process is >> >>> relatively easy due to NAFTA and the F1 visa. >> >>> >> >>> 4. How about techs that need sponsorship on an H-1 visa? I know alot >> >>> of companies shy away from this alternative because of the length of >> >>> time it can take to process a visa application but I think that if >>> you >> >>> take a look at the time it takes to find a tech at all against the >> >>> time it would take to process an H-1 visa it is quickly becoming 6 of > >>> one >> vs. >> >>> half dozen of another. I mean what difference does it make if it >> >>> takes up to 8 weeks to process an H-1 visa vs. 2-3 months to identify >> >>> a histology candidate? >> >>> >> >>> Your best bet is to get with your Human Resources department and >> >>> strategize, educate them on the challenges and shortages you are > facing. >> >>> Discuss some of these options or others you might come up with. >> >>> I hope this helps!! >> >>> >> >>> Thank You! >> >>> >> >>> >> >>> Pam Barker >> >>> President >> >>> RELIA >> >>> Specialists in Allied Healthcare Recruiting >> >>> 5703 Red Bug Lake Road #330 >> >>> Winter Springs, FL 32708-4969 >> >>> Phone: (407)657-2027 >> >>> Cell: (407)353-5070 >> >>> FAX: (407)678-2788 >> >>> E-mail: relia1@earthlink.net >> >>> _______________________________________________ >> >>> Histonet mailing list >> >>> Histonet@lists.utsouthwestern.edu >> >>> >> *http://lists.utsouthwestern.edu/mailman/listinfo/histonet*> ts.utsou thwestern.edu/mailman/listinfo/histonet> >> >>> >> >> >> >> >> >> _______________________________________________ >> >> Histonet mailing list >> >> Histonet@lists.utsouthwestern.edu >> >> *http://lists.utsouthwestern.edu/mailman/listinfo/histonet*> ts.utsou thwestern.edu/mailman/listinfo/histonet> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > > > > This e-mail message, including any attachments, is for the sole use of > the intended recipients and may contain privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. If > you are not the intended recipient, please contact the sender by e-mail > and destroy all copies of the original message, or you may call > PhenoPath Laboratories, Seattle, WA U.S.A. > at (206) 374-9000. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If > you are not the addressee intended / indicated or agent responsible for > delivering it to the addressee, you are hereby notified that you are in > possession of confidential and privileged information. Any dissemination, > distribution, or copying of this e-mail is strictly prohibited. If you have > received this message in error, please notify the sender immediately and > delete this email from your system. > > > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Judith Williams, PhD, HT(ASCP) Research Scientist Department of Comparative Medicine University of Washington Seattle, WA 98195 From gisela <@t> vetmed.wsu.edu Thu Apr 10 13:36:35 2008 From: gisela <@t> vetmed.wsu.edu (Bailey, Gisela) Date: Thu Apr 10 13:36:41 2008 Subject: [Histonet] Job posting In-Reply-To: <69272a080804091750h66cd0ddbj9abf11863885e45e@mail.gmail.com> References: <69272a080804091750h66cd0ddbj9abf11863885e45e@mail.gmail.com> Message-ID: <35CF12B690D8CA4E95375A36B4E7B44C0B6A78@cvm36.vetmed.wsu.edu> Histology Laboratory Manager, Washington Animal Disease Diagnostic Laboratory, College of Veterinary Medicine, Washington State University. This position is a full-time, administrative/professional position that will direct the histology laboratory operations. Application review begins April 30, 2008. For position description listing all minimum and preferred qualifications and application process please visit http://www.hrs.wsu.edu/employment/FAPvacancies.aspx (search # 5023) or email MLC@vetmed.wsu.edu. WSU is an EEO/AA educator and employer Gisela From christina.thurby <@t> bms.com Thu Apr 10 13:43:30 2008 From: christina.thurby <@t> bms.com (Christina Thurby) Date: Thu Apr 10 13:43:45 2008 Subject: [Histonet] fast/slow twitch myosin In-Reply-To: <200804101708.m3AH8qbP019404@meusplapp03.net.bms.com> References: <200804101708.m3AH8qbP019404@meusplapp03.net.bms.com> Message-ID: <47FE5FD2.7090202@bms.com> Vinnie, We've had really good success using antibodies from Sigma doing a double label in multiple species (rat, dog and NHP). Antibodies are: Slow Myosin, - Sigma clone NOQ7.5.4D 1:4000 Fast Myosin, - Sigma clone MY-32 1:300 You can contact me directly if you have any questions. Christina Thurby BMS 812-429-8097 histonet-request@lists.utsouthwestern.edu wrote: >Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > >To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > >You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > >When replying, please edit your Subject line so it is more specific >than "Re: Contents of Histonet digest..." > > >Today's Topics: > > 1. antibodies to myosin for fast twitch and slow twitch striated > muscle (Della Speranza, Vinnie) > 2. Histo vacancies (Martin, Gary) > 3. Re: Re: Nuclear Envelope/Membrane (Vernon Dailey) (Richard) > 4. RE: Histonet Vacancies (Morken, Tim) > 5. AW: [Histonet] antibodies to myosin for fast twitch and slow > twitchstriated muscle (Andi Kappeler) > > >---------------------------------------------------------------------- > >Message: 1 >Date: Thu, 10 Apr 2008 10:44:54 -0400 >From: "Della Speranza, Vinnie" >Subject: [Histonet] antibodies to myosin for fast twitch and slow > twitch striated muscle >To: "histonet@lists.utsouthwestern.edu" > >Message-ID: > >Content-Type: text/plain; charset="us-ascii" > >Can someone recommend a source for antibodies to myosin that will differentiate fast and slow twitch fibers? Preferably products you use and are happy with. > >Thank you, > >Vinnie Della Speranza >Manager for Anatomic Pathology Services >Medical University of South Carolina >165 Ashley Avenue Suite 309 >Charleston, South Carolina 29425 >Ph: (843) 792-6353 >Fax: (843) 792-8974 > > > > > >------------------------------ > >Message: 2 >Date: Thu, 10 Apr 2008 08:07:45 -0700 >From: "Martin, Gary" >Subject: [Histonet] Histo vacancies >To: >Message-ID: > <6ED9D4252F278841A0593D3D788AF24C02246F81@mailsvr.MARSHMED.local> >Content-Type: text/plain; charset="us-ascii" > >I have been reading these listing with great interest ... Dan's writing >prompted this listing. I am one of those people who fell into histology >because we did not have backup for our "certified" histo tech at our >pathology lab. I do not have the "proper education" to qualify for a >certification. The histo tech that I backup helped very little in my >training, and shares a bit of the attitude I read on this listing >sometimes, which is everybody should be certified or else they are not >credible techs. I came in on my weekends and after hours to train >myself on expired blocks, while putting myself through Freida Carson's >self instruction text. > > > >I do agree that it is probably best to be officially trained, but with >the shortage I believe that there should be some sort of variance to the >rules (there may be ... but I haven't found it). I presently work with >the local high schools in a program I helped developed called the Health >Academy, which introduces students to different areas of health care via >a shadowing program. The shadowing program and the presentations we >give in the class rooms provide a great opportunity to introduce these >student to histology. The question from these students is "where do you >get trained for this ,how long does it take, and what are the wages" >The answer to that question is usually not what they want to hear >(exampled by these listings). One kid told me "if you get accepted to >the plumbers training program through the union, you will start working >on the job immediately, and be earning about thirty to forty thousand a >year. Your requirements are, to attend school a couple of times a week >(in the evenings) for four years. After completion you are a journeyman >earning fifty to seventy thousand a year. Very hard to compete with >that ! > >I don't know what the answer is, but it seems to me the restriction are >getting tighter. I think there should be some levels of training >allowed with an easier way to move forward to deeper training on the >job. Thanks to all who have read and participated in this very >interesting discussion > >Gary > > > > > >------------------------------ > >Message: 3 >Date: Thu, 10 Apr 2008 16:19:52 +0100 >From: "Richard" >Subject: Re: [Histonet] Re: Nuclear Envelope/Membrane (Vernon Dailey) >To: >Message-ID: <000d01c89b1e$53560690$0200a8c0@delldim4500> >Content-Type: text/plain; charset="UTF-8" > >Eric said, and I respond: > > > >>>>Use POPO3 Iodide, it is a nuclear membrane stain. >>>> >>>> > >This is in live intact cells is it? >If so, have you a reference for this Eric, or indeed personal experience? I looked at the Invitrogen site for POPO3 [yes] and at their list of references and couldnt see anything plausible Do tell! > >Bye --- Richard Horobin > >Note: I'll be away at a conference, and hanging out with buddies, in Vermont from the 17th April to 6th May 2008. > >Academic: Div Neuroscience & Biomedical Systems, IBLS, University of Glasgow >Home: Springbank, 20 Tomcroy Terrace, Pitlochry PH16 5JA, Scotland >T 01796-474480 -- E RichardWHorobin@tomcroy.co.uk >"One does not need to live a burden-driven life" > >------------------------------ > >Message: 4 >Date: Thu, 10 Apr 2008 11:36:55 -0400 >From: "Morken, Tim" >Subject: RE: [Histonet] Histonet Vacancies >To: >Message-ID: > <6BFF6D137DF6BC43B33891BA96E83B190156C143@PGHCR-EXMB-VS-1.na.fshrnet.com> > >Content-Type: text/plain; charset="us-ascii" > > > Patty, Thanks for the link to the new program. And the histology "DSI" >game on the site is brilliant!! > > >Tim Morken > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti >Loykasek >Sent: Thursday, April 10, 2008 7:41 AM >To: Dan Dan; Histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] Histonet Vacancies > >Well said, Dan. I do have one small caveat, there is a new school in the >Northwest, Tacoma WA area. It is at Clover Park Technical College. Check >it out at www.cptc.edu. The area histotechs have been quite supportive, >and I think the program is off to a great start. > > >Patti Loykasek BS, HTL, QIHC >PhenoPath Laboratories >Seattle, WA > > > > > > >>I'll add my two-cents worth. It's hard to believe that after 25 years >>in this business that the histotech "shortage" situation is pretty >>much the same as it was long ago. There have certainly been >>improvements in education and training of people in histology, but for >> >> > > > >>the most part (99%??) people just fall into histology rather than pick >> >> > > > >>it at some point in school. In fact, it is a very rare day when I meet >> >> > > > >>someone who went to school for histotechnology, At this date there is >>still only one school on the west coast, and it is not the same school >> >> >that existed for a while in Seattle! > > >>The new one is in southern california. I looked into starting a >>histotechnology course here in the SF Bay Area, but it takes a long >>while with lots of justification and scrounging for equipment and if I >> >> > > > >>wanted to do it full time the pay would be half as much as I make now. >> >> > > > >>I could do it part time but then I'd be working 60-70 hours a week >>rather than 50 I do now. For the time being I give presentations to >>the local junior colleges. I may expand that to high schools. I know >>they are interested in getting speakers, but finding the time... >> >>I work in the biotech field as an IHC lab manger. We don't require >>HT/HTL/QIHC (though would love to have them!)but it is still very >>difficult to get experienced histotechs and nearly impossible to get >>experienced IHC techs around here. Most of the people I interview are >>from the research field who may have done some IHC at some point. And >>the ones who have good IHC experience are making very high salaries >>that our company gags at matching. It is not possible to recruit out >>of the Bay Area because the cost of housing is so high that no one >>that is not already living here can afford to move here. Even getting >>someone from southern cal is difficult - justifiying relocation >>expenses is difficult. It is kind of a joke that the experienced IHC >>techs know each other and spend their careers trading places at the >>varioius companies. Anthony is quite right that it takes a lot of >> >> >effort to educate HR and upper management about the need for higher pay. > > >>They really have a hard time justifying the same pay for a >>technologist that a new PhD will also make. >> >>On top of that we have loads of (legal) immigrants from Asia here and >>they are quite willing to work for lower pay to get a start. It is >>very common to have PhD level people, and even immigrant MD's (who >>can't get board certified here, mainly due to lanquage difficulties) >> >> >working as lab techs. > > >>Who is going to hire a AA/BS histotech when they can get a PhD who is >>happy to do the work and able to contribute to R&D at a higher level >>than most techs? >> >>There is a huge disparity between what hospitals and private >>diagnostic labs pay. The local Kaiser labs and Univeristy hospital >>labs pay in the $35 per hour range for basic histology. Senior IHC >>techs are in the $45 range. Those places have unions which is why the >>pay is so high. On the other hand, private labs will pay >>experieinced,certified people almost that much, but new, inexperienced >> >> > > > >>people may be paid $15-20/hr and the lab is not willing to help them >>get to their HT/HTL because they don't want to pay them more, and >>could lose them to the hospitals once trained. This is not speculation >> >> > > > >>but things I have had told to me first hand from techs who work in >> >> >these places. > > >>It is a little harder now for histotechs to be trained on the job due >>to the AA requirement. Most OJT people I have met were working in the >>hospital at some other job - lab assisant, dishwasher, etc before >>getting (accidently) into histology. A person working on an AA is >>probably not working in those jobs and probably does not even know a >> >> >histology lab exists. > > >>Obviously the need is to get exposure for histotechnology. The NSH >>makes brochures and videos available and does a career day for high >>schools students at the national meeting every year (I've helped at >>those). This is a good foundation. But it will really take every state >> >> > > > >>society and every interested histotech to do SOMETHING to promote the >>field. Science teachers at high schools are very interested in having >>working people come in to talk about a field. If someone in every city >> >> > > > >>could do that it would go a long way towards promoting the field. >>Maybe the key is to have NSH come up with a standardised presentation >>that anyone can give along with some props to make it a tactile >> >> >experience. > > >>But, after the promotion you have to realize that it takes a huge >>amount of initiative on the students part to follow through. They have >> >> > > > >>to start work on, or finish, an AA /BS degree, make contact with a lab >> >> > > > >>that is interested in training people, get the position, do on-the-job >> >> > > > >>(maybe at the same time as school) and THEN work on learning the >>matieral for an HT/HTL pretty much alone. Their facing a 3 to 4 year >>process. Longer if they get a bachelors degree. Key is finding labs to >> >> >training that value HT/HTL certification. > > >>Starting HT/HTL study groups would be a good way to get people >> >> >motivated. > > >>This is the kind of thing a state society can do. Individuals can to >>it as well, but it helps to have a group for motivation. >> >>Of course, that is why it has not happened - it depends too much on a >>few super-motivated individuals. The schools don't hear of a demand >>and so do not have programs. No programs means students never hear >>about it. Since students don't hear about it they can't even consider >> >> >it. A vicious circle. > > >>Imagine if the schools had students asking about courses leading to >>histotech certification or at least a chance at a job that leads to >>certification. That gets the schools attention. Junior colleges >>especially are very interested in providing trained people to the >>local job market. For them jobs = program justification. >> >>Think about it. >> >>Dan in Danville (a pseudonom to allow free speech) >> >> >> >> >> >> >>Dear Pam: >> >>For a long time histologists have gotten the short end of the stick. I >> >> > > > >>believe that the current situation is a symptom of the problem and is >>due to a history of underpayment. >> >>We cannot solve the problem by artificial means. This country is based >> >> > > > >>on the free market system. We must work with the system not against >>it, but first we must clearly understand the problem: Not enough >>techs, increasing barriers to entry into the profession, lack of >>visibility, no central planning to fix or even research the problem. >> >>We need to work to bring up the wages of HTs all across the country. >>If we do this, we will bring Histology to the attention of people in >>education at the moment. >> >>We can only get out of it by concentrating on increasing the number of >> >> > > > >>facilities that are prepared to take on new graduates and spend the >>time to get their practical skills up to speed. >> >>I think you are doing a valuable job bringing this situation back to >>the surface. We need to keep the momentum up and not let it die on the >> >> > > > >>vine of this valuable forum as a forgotten conversation. So, I ask my >>peers: What can we do to address this situation in an organized, >>effective manner? Who in our profession is responsible for being our >> >> >cheerleader? > > >>Finally, Pam, I want to say for myself that I encourage all >> >> >conversation. > > >>I will never be offended by a question, and I will always try to >>answer questions in a way that remembers there is a person behind it. >>No flame retardant needed here. >> >>I make my money from placing people on a temporary and permanent >> >> >basis. > > >>I am also a Histologist first and foremost and believe that patient >>safety is number one and understaffed laboratories experience more >> >> >mistakes. > > >>Keep up the good work, Pam. >> >>All my best, >> >>Anthony Williams BSc. HT >> >>Histotech Exchange LLC >> >>19 Whitmore St. >> >>Lexington, VA 24450 >> >>T 1 (302) 383 9780 >> >>F 1 (540) 463 3583 >> >>anthony@histotechexchange.com >> >> >> >>>I know this is a problem that has plagued facilities for years and I >>> >>> >>>too have noticed a change in the past 2 years. Yes, the histology >>> >>> >>>programs nationwide produce a great albeit small group of talented >>> >>> >>>people every year but the pool of available histo techs for permanent >>> >>> >>>positions has shrunk even more in recent years. At the risk of being >>> >>> >>>"flamed" by travel companies I have to say that you are losing alot >>>of >>> >>> >>>techs to travel positions. In the past 2 years of all of the histo >>> >>> >>>techs I have had contact with over half only want to work in >>>permanent >>> >>> >>>positions the rest either want to continue as travelers or become >>> >>> >>>travelers. Think about it... they get a higher rate of pay, benefits >>> >>> >>>and living expenses paid for. For these people it is a "better deal" >>> >>> >>>than committing to one facility. As a matter of fact it is a "better >>> >>> >>>deal" than a temp/travel position in any other field outside of >>> >>> >>>healthcare. Facilities who take the "quick solution" of hiring travel >>> >>> >>>techs are contributing to the shortage. May I offer some solutions? >>>Some >>> >>> >>creative hiring strategies? >> >> >> >>>Here are some ideas I would like to share: >>> >>> >>>1. If you are using travel techs do it with a temp to perm clause - >>> >>> >>>but be firm. If a tech works for you as a temp make sure they are at >>> >>> >>>least considering converting to a permanent employee at the end of >>>the >>> >>> >>>contract. If not don't extend, have your travel company send someone >>> >>> >>>else who would consider converting to a permanent position. And make >>> >>> >>>questions about their intentions part of your interview process the >>> >>> >>>same as you would if you were interviewing a candidate from out of >>> >>> >>>state for a permanent position. >>> >>> >>>2. Human Resources - Many of your allied health recruiters don't seem >>> >>> >>>to realize that histo techs don't grow on trees. So many times I see >>> >>> >>>facilities lose great techs because the hiring process has dragged >>>out >>> >>> >>>and the candidate ends up taking a position with a facility that can >>> >>> >>>move faster. Stay on top of your hr people especially once you know >>> >>> >>>they have a histology candidate. >>> >>> >>>3. How about techs from Canada? There are alot of talented techs in >>> >>> >>>Canada that are interested in moving to the states and the process is >>> >>> >>>relatively easy due to NAFTA and the F1 visa. >>> >>> >>>4. How about techs that need sponsorship on an H-1 visa? I know alot >>> >>> >>>of companies shy away from this alternative because of the length of >>> >>> >>>time it can take to process a visa application but I think that if >>>you >>> >>> >>>take a look at the time it takes to find a tech at all against the >>> >>> >>>time it would take to process an H-1 visa it is quickly becoming 6 of >>> >>> > > > >>>one >>> >>> >>vs. >> >> >> >>>half dozen of another. I mean what difference does it make if it >>> >>> >>>takes up to 8 weeks to process an H-1 visa vs. 2-3 months to identify >>> >>> >>>a histology candidate? >>> >>> >>>Your best bet is to get with your Human Resources department and >>> >>> >>>strategize, educate them on the challenges and shortages you are >>> >>> >facing. > > >>>Discuss some of these options or others you might come up with. >>> >>> >>>I hope this helps!! >>> >>> >>>Thank You! >>> >>> >>>Pam Barker >>> >>> >>>President >>> >>> >>>RELIA >>> >>> >>>Specialists in Allied Healthcare Recruiting >>> >>> >>>5703 Red Bug Lake Road #330 >>> >>> >>>Winter Springs, FL 32708-4969 >>> >>> >>>Phone: (407)657-2027 >>> >>> >>>Cell: (407)353-5070 >>> >>> >>>FAX: (407)678-2788 >>> >>> >>>E-mail: relia1@earthlink.net >>> >>> >>>_______________________________________________ >>> >>> >>>Histonet mailing list >>> >>> >>>Histonet@lists.utsouthwestern.edu >>> >>> >>*http://lists.utsouthwestern.edu/mailman/listinfo/histonet*>ts.utsou thwestern.edu/mailman/listinfo/histonet> >> >> >> >> >> >> >>_______________________________________________ >> >>Histonet mailing list >> >>Histonet@lists.utsouthwestern.edu >> >>*http://lists.utsouthwestern.edu/mailman/listinfo/histonet*>ts.utsou thwestern.edu/mailman/listinfo/histonet> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > > > > > > >This e-mail message, including any attachments, is for the sole use of >the intended recipients and may contain privileged information. Any >unauthorized review, use, disclosure or distribution is prohibited. If >you are not the intended recipient, please contact the sender by e-mail >and destroy all copies of the original message, or you may call >PhenoPath Laboratories, Seattle, WA U.S.A. >at (206) 374-9000. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >------------------------------ > >Message: 5 >Date: Thu, 10 Apr 2008 18:20:50 +0200 >From: "Andi Kappeler" >Subject: AW: [Histonet] antibodies to myosin for fast twitch and slow > twitchstriated muscle >To: "'Della Speranza, Vinnie'" , "Histonet" > >Message-ID: <003a01c89b26$d788abe0$17955c82@pi23> >Content-Type: text/plain; charset="iso-8859-1" > >Hi Vinnie > >We routinely use the following mouse monoclonal antibodies on FFPE human >skeletal muscle: >Myosin I (slow), clone NOQ7.5.4D, Sigma M-8421; working conc. 5 ug Ig/ml, >HIER in 10 mM citrate buffer, pH 6.0, pressure cooker. >Myosin II (fast), clone MY-31, Sigma M.4276; working conc. 10 ug Ig/ml, HIER >as above. >Hope this helps. > >Andi Kappeler >Insitute of Pathology, University of Bern, Switzerland > >-----Urspr?ngliche Nachricht----- >Von: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Della >Speranza, Vinnie >Gesendet: Donnerstag, 10. April 2008 16:45 >An: histonet@lists.utsouthwestern.edu >Betreff: [Histonet] antibodies to myosin for fast twitch and slow >twitchstriated muscle > >Can someone recommend a source for antibodies to myosin that will >differentiate fast and slow twitch fibers? Preferably products you use and >are happy with. > >Thank you, > >Vinnie Della Speranza >Manager for Anatomic Pathology Services >Medical University of South Carolina >165 Ashley Avenue Suite 309 >Charleston, South Carolina 29425 >Ph: (843) 792-6353 >Fax: (843) 792-8974 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > >------------------------------ > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >End of Histonet Digest, Vol 53, Issue 16 >**************************************** > > From Eric.Mahoney <@t> cchmc.org Thu Apr 10 13:57:23 2008 From: Eric.Mahoney <@t> cchmc.org (Eric Mahoney) Date: Thu Apr 10 13:57:54 2008 Subject: [Histonet] Re: Nuclear Envelope/Membrane (Vernon Dailey) (Richard) Message-ID: <47FE2AD40200009700013624@n6mcgw16.cchmc.org> >>> 04/10/08 1:04 PM >>> Richard, I do have personal experience with Popo3 Iodide. In fact in an hour from now I will be staining with it... But, our cells are fixed in 5% TCA for 48 hours in 4degree and then frozen sections. Our Popo3 is from Invitrogen Cat #P3584. I do not have a reference at hand right now other than I use it and you can do a PubMed search for Popo3 Iodide as well. If you are doing Immunostaining, you can not use Popo3 and Cy3 2Ab because they light up the same channel in confocal. I use Popo3 and Cy5 2Ab. I hope this all helps! Feel free to email me with further questions: Eric.Mahoney@cchmc.org Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. antibodies to myosin for fast twitch and slow twitch striated muscle (Della Speranza, Vinnie) 2. Histo vacancies (Martin, Gary) 3. Re: Re: Nuclear Envelope/Membrane (Vernon Dailey) (Richard) 4. RE: Histonet Vacancies (Morken, Tim) 5. AW: [Histonet] antibodies to myosin for fast twitch and slow twitchstriated muscle (Andi Kappeler) ---------------------------------------------------------------------- Message: 1 Date: Thu, 10 Apr 2008 10:44:54 -0400 From: "Della Speranza, Vinnie" Subject: [Histonet] antibodies to myosin for fast twitch and slow twitch striated muscle To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Can someone recommend a source for antibodies to myosin that will differentiate fast and slow twitch fibers? Preferably products you use and are happy with. Thank you, Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Ph: (843) 792-6353 Fax: (843) 792-8974 ------------------------------ Message: 2 Date: Thu, 10 Apr 2008 08:07:45 -0700 From: "Martin, Gary" Subject: [Histonet] Histo vacancies To: Message-ID: <6ED9D4252F278841A0593D3D788AF24C02246F81@mailsvr.MARSHMED.local> Content-Type: text/plain; charset="us-ascii" I have been reading these listing with great interest ... Dan's writing prompted this listing. I am one of those people who fell into histology because we did not have backup for our "certified" histo tech at our pathology lab. I do not have the "proper education" to qualify for a certification. The histo tech that I backup helped very little in my training, and shares a bit of the attitude I read on this listing sometimes, which is everybody should be certified or else they are not credible techs. I came in on my weekends and after hours to train myself on expired blocks, while putting myself through Freida Carson's self instruction text. I do agree that it is probably best to be officially trained, but with the shortage I believe that there should be some sort of variance to the rules (there may be ... but I haven't found it). I presently work with the local high schools in a program I helped developed called the Health Academy, which introduces students to different areas of health care via a shadowing program. The shadowing program and the presentations we give in the class rooms provide a great opportunity to introduce these student to histology. The question from these students is "where do you get trained for this ,how long does it take, and what are the wages" The answer to that question is usually not what they want to hear (exampled by these listings). One kid told me "if you get accepted to the plumbers training program through the union, you will start working on the job immediately, and be earning about thirty to forty thousand a year. Your requirements are, to attend school a couple of times a week (in the evenings) for four years. After completion you are a journeyman earning fifty to seventy thousand a year. Very hard to compete with that ! I don't know what the answer is, but it seems to me the restriction are getting tighter. I think there should be some levels of training allowed with an easier way to move forward to deeper training on the job. Thanks to all who have read and participated in this very interesting discussion Gary ------------------------------ Message: 3 Date: Thu, 10 Apr 2008 16:19:52 +0100 From: "Richard" Subject: Re: [Histonet] Re: Nuclear Envelope/Membrane (Vernon Dailey) To: Message-ID: <000d01c89b1e$53560690$0200a8c0@delldim4500> Content-Type: text/plain; charset="UTF-8" Eric said, and I respond: >>> Use POPO3 Iodide, it is a nuclear membrane stain. This is in live intact cells is it? If so, have you a reference for this Eric, or indeed personal experience? I looked at the Invitrogen site for POPO3 [yes] and at their list of references and couldnt see anything plausible Do tell! Bye --- Richard Horobin Note: I'll be away at a conference, and hanging out with buddies, in Vermont from the 17th April to 6th May 2008. Academic: Div Neuroscience & Biomedical Systems, IBLS, University of Glasgow Home: Springbank, 20 Tomcroy Terrace, Pitlochry PH16 5JA, Scotland T 01796-474480 -- E RichardWHorobin@tomcroy.co.uk "One does not need to live a burden-driven life" ------------------------------ Message: 4 Date: Thu, 10 Apr 2008 11:36:55 -0400 From: "Morken, Tim" Subject: RE: [Histonet] Histonet Vacancies To: Message-ID: <6BFF6D137DF6BC43B33891BA96E83B190156C143@PGHCR-EXMB-VS-1.na.fshrnet.com> Content-Type: text/plain; charset="us-ascii" Patty, Thanks for the link to the new program. And the histology "DSI" game on the site is brilliant!! Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti Loykasek Sent: Thursday, April 10, 2008 7:41 AM To: Dan Dan; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Histonet Vacancies Well said, Dan. I do have one small caveat, there is a new school in the Northwest, Tacoma WA area. It is at Clover Park Technical College. Check it out at www.cptc.edu. The area histotechs have been quite supportive, and I think the program is off to a great start. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > I'll add my two-cents worth. It's hard to believe that after 25 years > in this business that the histotech "shortage" situation is pretty > much the same as it was long ago. There have certainly been > improvements in education and training of people in histology, but for > the most part (99%??) people just fall into histology rather than pick > it at some point in school. In fact, it is a very rare day when I meet > someone who went to school for histotechnology, At this date there is > still only one school on the west coast, and it is not the same school that existed for a while in Seattle! > The new one is in southern california. I looked into starting a > histotechnology course here in the SF Bay Area, but it takes a long > while with lots of justification and scrounging for equipment and if I > wanted to do it full time the pay would be half as much as I make now. > I could do it part time but then I'd be working 60-70 hours a week > rat> the local junior colleges. I may expand that to high schools. I know > they are interested in getting speakers, but finding the time... > > I work in the biotech field as an IHC lab manger. We don't require > HT/HTL/QIHC (though would love to have them!)but it is still very > difficult to get experienced histotechs and nearly impossible to get > experienced IHC techs around here. Most of the people I interview are > from the research field who may have done some IHC at some point. And > the ones who have good IHC experience are making very high salaries > that our company gags at matching. It is not possible to recruit out > of the Bay Area because the cost of housing is so high that no one > that is not already living here can afford to move here. Even getting > someone from southern cal is difficult - justifiying relocation > expenses is difficult. It is kind of a joke that the experienced IHC > techs know each other and spend their careers trading places at the > varioius companies. Anthony is quite right that it takes a lot of effort to educate HR and upper management about the need for higher pay. > They really have a hard time justifying the same pay for a > technologist that a new PhD will also make. > > On top of that we have loads of (legal) immigrants from Asia here and > they are quite willing to work for lower pay to get a start. It is > very common to have PhD level people, and even immigrant MD's (who > can't get board certified here, mainly due to lanquage difficulties) working as lab techs. > Who is going to hire a AA/BS histotech when they can get a PhD who is > happy to do the work and able to contribute to R&D at a higher level > than most techs? > > There is a huge disparity between what hospitals and private > diagnostic labs pay. The local Kaiser labs and Univeristy hospital > labs pay in the $35 per hour range for basic histology. Senior IHC > techs are in the $45 range. Those places have unions which is why the > pay is so high. On the other hand, private labs will pay > experieinced,certified people almost that much, but new, inexperienced > people may be paid $15-20/hr and the lab is not willing to help them > get to their HT/HTL because they don't want to pay them more, and > could lose them to the hospitals once trained. This is not speculation > but things I have had told to me first hand from techs who work in these places. > > It is a little harder now for histotechs to be trained on the job due > to the AA requirement. Most OJT people I have met were working in the > hospital at some other job - lab assisant, dishwasher, etc before > getting (accidently) into histology. A person working on an AA is > probably not working in those jobs and probably does not even know a histology lab exists. > > Obviously the need is to get exposure for histotechnology. The NSH > makes brochures and videos available and does a career day for high > schools students at the national meeting every year (I've helped at > those). This is a good foundation. But it will really take every state > society and every interested histotech to do SOMETHING to promote the > field. Science teachers at high schools are very interested in having > working people come in to talk about a field. If someone in every city > could do that it would go a long way towards promoting the field. > Maybe the key is to have NSH come up with a standardised presentation > that anyone can give along with some props to make it a tactile experience. > > But, after the promotion you have to realize that it takes a huge > amount of initiative on the students part to follow through. They have > to start work on, or finish, an AA /BS degree, make contact with a lab > that is interested in training people, get the position, do on-the-job > (maybe at the same time as school) and THEN work on learning the > matieral for an HT/HTL pretty much alone. Their facing a 3 to 4 year > process. Longer if they get a bachelors degree> Starting HT/HTL study groups would be a good way to get people motivated. > This is the kind of thing a state society can do. Individuals can to > it as well, but it helps to have a group for motivation. > > Of course, that is why it has not happened - it depends too much on a > few super-motivated individuals. The schools don't hear of a demand > and so do not have programs. No programs means students never hear > about it. Since students don't hear about it they can't even consider it. A vicious circle. > > Imagine if the schools had students asking about courses leading to > histotech certification or at least a chance at a job that leads to > certification. That gets the schools attention. Junior colleges > especially are very interested in providing trained people to the > local job market. For them jobs = program justification. > > Think about it. > > Dan in Danville (a pseudonom to allow free speech) > > > > > > > Dear Pam: > > For a long time histologists have gotten the short end of the stick. I > believe that the current situation is a symptom of the problem and is > due to a history of underpayment. > > We cannot solve the problem by artificial means. This country is based > on the free market system. We must work with the system not against > it, but first we must clearly understand the problem: Not enough > techs, increasing barriers to entry into the profession, lack of > visibility, no central planning to fix or even research the problem. > > We need to work to bring up the wages of HTs all across the country. > If we do this, we will bring Histology to the attention of people in > education at the moment. > > We can only get out of it by concentrating on increasing the number of > facilities that are prepared to take on new graduates and spend the > time to get their practical skills up to speed. > > I think you are doing a valuable job bringing this situation back to > the surface. We need to keep the momentum up and not let it die on the > vine of this valuable forum as a forgotten conversation. So, I ask my > peers: What can we do to address this situation in an organized, > effective manner? Who in our profession is responsible for being our cheerleader? > > Finally, Pam, I want to say for myself that I encourage all conversation. > > I will never be offended by a question, and I will always try to > answer questions in a way that remembers there is a person behind it. > No flame retardant needed here. > > I make my money from placing people on a temporary and permanent basis. > > I am also a Histologist first and foremost and believe that patient > safety is number one and understaffed laboratories experience more mistakes. > > Keep up the good work, Pam. > > All my best, > > Anthony Williams BSc. HT > > Histotech Exchange LLC > > 19 Whitmore St. > > Lexington, VA 24450 > > T 1 (302) 383 9780 > > F 1 (540) 463 3583 > > anthony@histotechexchange.com > >> I know this is a problem that has plagued facilities for years and I > >> too have noticed a change in the past 2 years. Yes, the histology > >> programs nationwide produce a great albeit small group of talented > >> people every year but the pool of available histo techs for permanent > >> positions has shrunk even more in recent years. At the risk of being > >> "flamed" by travel companies I have to say that you are losing alot >> of > >> techs to travel positions. In the past 2 years of all of the histo > >> techs I have had contact with over half only want to work in >> permanent > >> positions the rest either want to continue as travelers or become > >> travelers. Think about it... they get a higher rate of pay, benefits > >> and living expenses paid for. For these people it is a "better deal" > >> than committing to one facility. As a matter of fact it is a "better > >> deal" than a temp/travel position in any other field outside of > >> healthcare. Faciliti>> techs are contributing to the shortage. May I offer some solutions? >> Some > creative hiring strategies? > >> > >> Here are some ideas I would like to share: > >> 1. If you are using travel techs do it with a temp to perm clause - > >> but be firm. If a tech works for you as a temp make sure they are at > >> least considering converting to a permanent employee at the end of >> the > >> contract. If not don't extend, have your travel company send someone > >> else who would consider converting to a permanent position. And make > >> questions about their intentions part of your interview process the > >> same as you would if you were interviewing a candidate from out of > >> state for a permanent position. > >> > >> 2. Human Resources - Many of your allied health recruiters don't seem > >> to realize that histo techs don't grow on trees. So many times I see > >> facilities lose great techs because the hiring process has dragged >> out > >> and the candidate ends up taking a position with a facility that can > >> move faster. Stay on top of your hr people especially once you know > >> they have a histology candidate. > >> > >> 3. How about techs from Canada? There are alot of talented techs in > >> Canada that are interested in moving to the states and the process is > >> relatively easy due to NAFTA and the F1 visa. > >> > >> 4. How about techs that need sponsorship on an H-1 visa? I know alot > >> of companies shy away from this alternative because of the length of > >> time it can take to process a visa application but I think that if >> you > >> take a look at the time it takes to find a tech at all against the > >> time it would take to process an H-1 visa it is quickly becoming 6 of >> one > vs. > >> half dozen of another. I mean what difference does it make if it > >> takes up to 8 weeks to process an H-1 visa vs. 2-3 months to identify > >> a histology candidate? > >> > >> Your best bet is to get with your Human Resources department and > >> strategize, educate them on the challenges and shortages you are facing. > >> Discuss some of these options or others you might come up with. > >> I hope this helps!! > >> > >> Thank You! > >> > >> > >> Pam Barker > >> President > >> RELIA > >> Specialists in Allied Healthcare Recruiting > >> 5703 Red Bug Lake Road #330 > >> Winter Springs, FL 32708-4969 > >> Phone: (407)657-2027 > >> Cell: (407)353-5070 > >> FAX: (407)678-2788 > >> E-mail: relia1@earthlink.net > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> > *http://lists.utsouthwestern.edu/mailman/listinfo/histonet* ts.utsou thwestern.edu/mailman/listinfo/histonet> > >> > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > *http://lists.utsouthwestern.edu/mailman/listinfo/histonet* ts.utsou thwestern.edu/mailman/listinfo/histonet> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Thu, 10 Apr 2008 18:20:50 +0200 From: "Andi Kappeler" Subject: AW: [Histonet] antibodies to myosin for fast twitch and slow twitchstriated muscle To: "'Della Speranza, Vinnie'" palmbeachpath.com Thu Apr 10 14:03:09 2008 From: JCollins <@t> palmbeachpath.com (Judy Collins) Date: Thu Apr 10 14:03:15 2008 Subject: [Histonet] Salary / Temp positions In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C0228415E@mailsvr.MARSHMED.local> References: <5d9104a30804101012y48035d2cwefc3148107838ed9@mail.gmail.com> <05CAE76AB5D5ED409864C6DD86F13349018863FF64@pbpsflexch02.pbp.local> <6ED9D4252F278841A0593D3D788AF24C0228415E@mailsvr.MARSHMED.local> Message-ID: <05CAE76AB5D5ED409864C6DD86F13349018863FF6D@pbpsflexch02.pbp.local> I don't think that is correct. Our current student does not have an AA, will finish the program in May and will be taking the ASCP. I think they were talking about requiring an AA but that never was instituted officially. Judy Collins -----Original Message----- From: Martin, Gary [mailto:gmartin@marshallmedical.org] Sent: Thursday, April 10, 2008 2:18 PM To: Judy Collins Subject: RE: [Histonet] Salary / Temp positions I believe you have to have the minium of an AA Gary -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Judy Collins Sent: Thursday, April 10, 2008 10:37 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Salary / Temp positions What about the nontraditional learning programs such as Indiana University's? Our laboratory has helped 5 prospective techs through the past few years to become histotechnicians through their program. The program is 10 months long at a very reasonable cost and the candidates are eligible to sit for the ASCP at the end of the program. I believe there are some other similar programs as well. Judy Collins Palm Beach Pathology -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy DuBois Sent: Thursday, April 10, 2008 1:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Salary / Temp positions If we think about the cost of 2-4 years of college to obtain a degree in order to qualify for the Histology test, most of us would be in debt when finished. Then look at our salary and you can see how the requirements just aren't supported by the salary. Most of the students will come out of college owing on student loans. The salaries they would receive as new histotech would allow them to pay off their student loanswhile maintaining a decent living (at least here in CA). With both my sons in college (using student loans) we had to take a serious look at the final amount they will owe when the graduate and compare it to what they would be earning. I am not sure what the solution is. Any ideas? Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Thu Apr 10 14:35:29 2008 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Thu Apr 10 14:35:32 2008 Subject: [Histonet] Histology vacancies Message-ID: <842113.49682.qm@web31305.mail.mud.yahoo.com> Hello Dan in Danville, Having worked and lived in the SF Bay Area for a number of years, and in Danville too, I know exactly what you are talking about! I agree with everything you and Patti stated. You may or may not be aware of it, but Governor Arnold Schwarzenegger has mandated that ALL laboratory staff working in health care, be certified by 2009, or was it 2010. This includes histologists. I know of a number of histologists that are not certified in CA, and this is going to cause a huge problem, as well as further shortages in histology laboratories. NY has been dealing with this issue too. Akemi Allison-Tacha, BS, HT(ASCP)HTL Client Services Manager PhenoPath laboratories 551 North 34th Street, Suite 100 Seattle, WA 98103-8675 Work: (206) 374-9000 E-Mail: akemiat3377@yahoo.com From elizabeth.heimrich <@t> bms.com Thu Apr 10 14:35:33 2008 From: elizabeth.heimrich <@t> bms.com (Elizabeth M Heimrich) Date: Thu Apr 10 14:35:46 2008 Subject: [Histonet] Salary / Temp positions In-Reply-To: <05CAE76AB5D5ED409864C6DD86F13349018863FF64@pbpsflexch02.pbp.local> References: <5d9104a30804101012y48035d2cwefc3148107838ed9@mail.gmail.com> <05CAE76AB5D5ED409864C6DD86F13349018863FF64@pbpsflexch02.pbp.local> Message-ID: <47FE6C05.1060709@bms.com> I took that course!! It is a very informative independant learning exercise, but they have a set of prerequisites such as biology and chemistry. Thenin lies the problem for a lot of OJT Histotechs. They have the experience, but they don't have the college courses. We have a problem, so now what do we do?!? Personally I think the techs should have the bio and chem background because a lot of techs who are not science savy have done some harmful mistakes. For example, I have seen a lab aid dump unneutralized formalin waste down the drain. Another tech thought it was OK to store acids and bases in the same cabinet, and even another person with no chem background made a batch of acid alcohol with 10% NBF!! My slides that day looked like garbage!! No wonder the safety cops have cracked down!!! Just my 2 cents, Beth Judy Collins wrote: >What about the nontraditional learning programs such as Indiana University's? Our laboratory has helped 5 prospective techs through the past few years to become histotechnicians through their program. The program is 10 months long at a very reasonable cost and the candidates are eligible to sit for the ASCP at the end of the program. I believe there are some other similar programs as well. > >Judy Collins >Palm Beach Pathology > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy DuBois >Sent: Thursday, April 10, 2008 1:13 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Salary / Temp positions > >If we think about the cost of 2-4 years of college to obtain a degree in >order to qualify for the Histology test, most of us would be in debt when >finished. Then look at our salary and you can see how the requirements just >aren't supported by the salary. Most of the students will come out of >college owing on student loans. The salaries they would receive as new >histotech would allow them to pay off their student loanswhile maintaining a >decent living (at least here in CA). >With both my sons in college (using student loans) we had to take a serious >look at the final amount they will owe when the graduate and compare it to >what they would be earning. >I am not sure what the solution is. Any ideas? > >Cindy >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From ryaskovich <@t> dir.nidcr.nih.gov Thu Apr 10 14:45:45 2008 From: ryaskovich <@t> dir.nidcr.nih.gov (Yaskovich, Ruth A (NIH/NIDCR) [E]) Date: Thu Apr 10 14:45:54 2008 Subject: [Histonet] Histology vacancies In-Reply-To: <842113.49682.qm@web31305.mail.mud.yahoo.com> References: <842113.49682.qm@web31305.mail.mud.yahoo.com> Message-ID: I'd like to know what Schwarzenegger's degree is in? Ruth Yaskovich N.I.H. -----Original Message----- From: Akemi Allison-Tacha [mailto:akemiat3377@yahoo.com] Sent: Thursday, April 10, 2008 3:35 PM To: Histonet Subject: [Histonet] Histology vacancies Hello Dan in Danville, Having worked and lived in the SF Bay Area for a number of years, and in Danville too, I know exactly what you are talking about! I agree with everything you and Patti stated. You may or may not be aware of it, but Governor Arnold Schwarzenegger has mandated that ALL laboratory staff working in health care, be certified by 2009, or was it 2010. This includes histologists. I know of a number of histologists that are not certified in CA, and this is going to cause a huge problem, as well as further shortages in histology laboratories. NY has been dealing with this issue too. Akemi Allison-Tacha, BS, HT(ASCP)HTL Client Services Manager PhenoPath laboratories 551 North 34th Street, Suite 100 Seattle, WA 98103-8675 Work: (206) 374-9000 E-Mail: akemiat3377@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Thu Apr 10 14:52:03 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Apr 10 14:52:15 2008 Subject: [Histonet] Salary / Temp positions In-Reply-To: <05CAE76AB5D5ED409864C6DD86F13349018863FF6D@pbpsflexch02.pbp.local> Message-ID: <002901c89b44$5c9ccb00$d00f7ca5@lurie.northwestern.edu> Indiana university also has an associate of science in histotechnology as well as the certification program. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Judy Collins Sent: Thursday, April 10, 2008 2:03 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Salary / Temp positions I don't think that is correct. Our current student does not have an AA, will finish the program in May and will be taking the ASCP. I think they were talking about requiring an AA but that never was instituted officially. Judy Collins -----Original Message----- From: Martin, Gary [mailto:gmartin@marshallmedical.org] Sent: Thursday, April 10, 2008 2:18 PM To: Judy Collins Subject: RE: [Histonet] Salary / Temp positions I believe you have to have the minium of an AA Gary -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Judy Collins Sent: Thursday, April 10, 2008 10:37 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Salary / Temp positions What about the nontraditional learning programs such as Indiana University's? Our laboratory has helped 5 prospective techs through the past few years to become histotechnicians through their program. The program is 10 months long at a very reasonable cost and the candidates are eligible to sit for the ASCP at the end of the program. I believe there are some other similar programs as well. Judy Collins Palm Beach Pathology -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy DuBois Sent: Thursday, April 10, 2008 1:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Salary / Temp positions If we think about the cost of 2-4 years of college to obtain a degree in order to qualify for the Histology test, most of us would be in debt when finished. Then look at our salary and you can see how the requirements just aren't supported by the salary. Most of the students will come out of college owing on student loans. The salaries they would receive as new histotech would allow them to pay off their student loanswhile maintaining a decent living (at least here in CA). With both my sons in college (using student loans) we had to take a serious look at the final amount they will owe when the graduate and compare it to what they would be earning. I am not sure what the solution is. Any ideas? Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cory.collins <@t> DHAT.com Thu Apr 10 14:52:30 2008 From: cory.collins <@t> DHAT.com (Cory Collins) Date: Thu Apr 10 14:52:26 2008 Subject: [Histonet] Salary / Temp positions Message-ID: Hey Cindy, I think you have a valid point with the cost of 2-4 years worth of schooling. It is very expensive. But we're not the only ones faced with the problem of low pay out of college and student loans. Teachers get paid far less than what they deserve and they still do it and there is a huge shortage of teachers, just like techs. This is where we are at in the profession and it's not going to change, this is the answer that the powers that be have come up with. I think it's important for a tech to have a strong background in science. This will certainly help them to be able to troubleshoot problems in the lab. The histology world is getting much more complex with the use of IHC, ISH, FISH, image analysis and whatever else is on the horizon. I'm not saying that a tech that doesn't have formal training can't learn these things on the job, I've taught a few techs these areas that didn't know squat about science before coming into the lab and they've done great. But to improve our pay over the next couple of decades, I think ASCP is right on with the requirements. Unfortunately that means a shortage of techs and it'll probably be that way for the next several years. Our answer to ASCP's requirements is getting the word out to anyone that will listen about histology as a career, especially young people. I graduated almost 9 years ago and am still paying on my student loans, I have a ways to go. The good news is the lenders give you plenty of time to do it and you can get on a payment program where the payments start low and increase over time. This allows you to make a living right out of school and then pay more when you should be making more, a few years after graduation. Just my two cents...I think a college degree is well worth the price of admission. The experience along with the long-term earning potential makes it a good investment. Cory Collins, HT (ASCP) QIHC Histology Lab Supervisor Digestive Health Associates of Texas 7920 Elmbrook Dr, Suite 104 Dallas, TX 75247 P: (214)689-5960 x 311 F: (214)689-3804 www.DHAT.com Date: Thu, 10 Apr 2008 10:12:54 -0700 From: "Cindy DuBois" Subject: [Histonet] Salary / Temp positions To: histonet@lists.utsouthwestern.edu Message-ID: <5d9104a30804101012y48035d2cwefc3148107838ed9@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 If we think about the cost of 2-4 years of college to obtain a degree in order to qualify for the Histology test, most of us would be in debt when finished. Then look at our salary and you can see how the requirements just aren't supported by the salary. Most of the students will come out of college owing on student loans. The salaries they would receive as new histotech would allow them to pay off their student loanswhile maintaining a decent living (at least here in CA). With both my sons in college (using student loans) we had to take a serious look at the final amount they will owe when the graduate and compare it to what they would be earning. I am not sure what the solution is. Any ideas? Cindy From Heather.D.Renko <@t> osfhealthcare.org Thu Apr 10 15:06:35 2008 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Thu Apr 10 15:06:51 2008 Subject: [Histonet] RE: Instrumentation IHC Message-ID: <40026EDDE64CDA47AB382C52619ACD3C0A09DE59@pmc-rfd-mx01.intranet.osfnet.org> We have the Ventana Benchmark & Benchmark XT in our lab and love it. The tech support is GREAT! Have also heard good things about the Biocare Nemesis and the Leica Bond Max system Heather D. Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 Direct: 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From juditw <@t> u.washington.edu Thu Apr 10 15:49:08 2008 From: juditw <@t> u.washington.edu (Judith L. Williams) Date: Thu Apr 10 15:49:16 2008 Subject: [Histonet] RE: Histology CSE- Dead Skunk Inquiries! In-Reply-To: Message-ID: Greetings histo world - just a fix it note- Jason did not work on the project it was Brandon Rogers. Sorry - got the computer guys confused with the Flash video folks - must be my non- computer background! sorry Brandon! anyway - he did a great job on it! Judith Thu, 10 Apr 2008, Judith L. Williams wrote: > HI - I am Judith Williams, the creator of DSI!!! along with Jason Kruse. I > was the instructor at Clover Park Tech College last year and then I took > another job at Univ of WAshington. I designed the idea and text along with > Jason, one of their computer experts at the college. He did a fantastic job of > making it interesting! He came to the lab and took photos and I explained each > step- he took notes then made the cartoons and applied it. He and I worked on > it, editing and tweeking it - he is so clever! I really want him to get a lot > of credit for it. Isn't it wonderful! He and I are going to make CD's of it to > send out for advertising histotechnology. Will let you know about when they > are going to be ready. > Glad you all enjoyed it. I am a huge CSI fan - so naturally we did that twist! > cheers to all histoworld!!! We need to sell this career - its great! > > > > Judith Williams, PhD, HT(ASCP) > Department of Comparative Medicine > University of Washington > Seattle, WA > > > > On Thu, 10 Apr 2008, Cheri Miller wrote: > >> I agree, its very clever! I have saved it to share with my department! >> Cheryl Miller HT (ASCP) >> Histology Supervisor >> Physicians Laboratory,P.C. >> Omaha, Ne. >> 402 738 5052 >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim >> Sent: Thursday, April 10, 2008 10:37 AM >> To: Histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] Histonet Vacancies >> >> >> Patty, Thanks for the link to the new program. And the histology "DSI" >> game on the site is brilliant!! >> >> >> Tim Morken >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti >> Loykasek >> Sent: Thursday, April 10, 2008 7:41 AM >> To: Dan Dan; Histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] Histonet Vacancies >> >> Well said, Dan. I do have one small caveat, there is a new school in the >> Northwest, Tacoma WA area. It is at Clover Park Technical College. Check >> it out at www.cptc.edu. The area histotechs have been quite supportive, >> and I think the program is off to a great start. >> >> >> Patti Loykasek BS, HTL, QIHC >> PhenoPath Laboratories >> Seattle, WA >> >> >> >> >>> I'll add my two-cents worth. It's hard to believe that after 25 years >>> in this business that the histotech "shortage" situation is pretty >>> much the same as it was long ago. There have certainly been >>> improvements in education and training of people in histology, but for >> >>> the most part (99%??) people just fall into histology rather than pick >> >>> it at some point in school. In fact, it is a very rare day when I meet >> >>> someone who went to school for histotechnology, At this date there is >>> still only one school on the west coast, and it is not the same school >> that existed for a while in Seattle! >>> The new one is in southern california. I looked into starting a >>> histotechnology course here in the SF Bay Area, but it takes a long >>> while with lots of justification and scrounging for equipment and if I >> >>> wanted to do it full time the pay would be half as much as I make now. >> >>> I could do it part time but then I'd be working 60-70 hours a week >>> rather than 50 I do now. For the time being I give presentations to >>> the local junior colleges. I may expand that to high schools. I know >>> they are interested in getting speakers, but finding the time... >>> >>> I work in the biotech field as an IHC lab manger. We don't require >>> HT/HTL/QIHC (though would love to have them!)but it is still very >>> difficult to get experienced histotechs and nearly impossible to get >>> experienced IHC techs around here. Most of the people I interview are >>> from the research field who may have done some IHC at some point. And >>> the ones who have good IHC experience are making very high salaries >>> that our company gags at matching. It is not possible to recruit out >>> of the Bay Area because the cost of housing is so high that no one >>> that is not already living here can afford to move here. Even getting >>> someone from southern cal is difficult - justifiying relocation >>> expenses is difficult. It is kind of a joke that the experienced IHC >>> techs know each other and spend their careers trading places at the >>> varioius companies. Anthony is quite right that it takes a lot of >> effort to educate HR and upper management about the need for higher pay. >>> They really have a hard time justifying the same pay for a >>> technologist that a new PhD will also make. >>> >>> On top of that we have loads of (legal) immigrants from Asia here and >>> they are quite willing to work for lower pay to get a start. It is >>> very common to have PhD level people, and even immigrant MD's (who >>> can't get board certified here, mainly due to lanquage difficulties) >> working as lab techs. >>> Who is going to hire a AA/BS histotech when they can get a PhD who is >>> happy to do the work and able to contribute to R&D at a higher level >>> than most techs? >>> >>> There is a huge disparity between what hospitals and private >>> diagnostic labs pay. The local Kaiser labs and Univeristy hospital >>> labs pay in the $35 per hour range for basic histology. Senior IHC >>> techs are in the $45 range. Those places have unions which is why the >>> pay is so high. On the other hand, private labs will pay >>> experieinced,certified people almost that much, but new, inexperienced >> >>> people may be paid $15-20/hr and the lab is not willing to help them >>> get to their HT/HTL because they don't want to pay them more, and >>> could lose them to the hospitals once trained. This is not speculation >> >>> but things I have had told to me first hand from techs who work in >> these places. >>> >>> It is a little harder now for histotechs to be trained on the job due >>> to the AA requirement. Most OJT people I have met were working in the >>> hospital at some other job - lab assisant, dishwasher, etc before >>> getting (accidently) into histology. A person working on an AA is >>> probably not working in those jobs and probably does not even know a >> histology lab exists. >>> >>> Obviously the need is to get exposure for histotechnology. The NSH >>> makes brochures and videos available and does a career day for high >>> schools students at the national meeting every year (I've helped at >>> those). This is a good foundation. But it will really take every state >> >>> society and every interested histotech to do SOMETHING to promote the >>> field. Science teachers at high schools are very interested in having >>> working people come in to talk about a field. If someone in every city >> >>> could do that it would go a long way towards promoting the field. >>> Maybe the key is to have NSH come up with a standardised presentation >>> that anyone can give along with some props to make it a tactile >> experience. >>> >>> But, after the promotion you have to realize that it takes a huge >>> amount of initiative on the students part to follow through. They have >> >>> to start work on, or finish, an AA /BS degree, make contact with a lab >> >>> that is interested in training people, get the position, do on-the-job >> >>> (maybe at the same time as school) and THEN work on learning the >>> matieral for an HT/HTL pretty much alone. Their facing a 3 to 4 year >>> process. Longer if they get a bachelors degree. Key is finding labs to >> training that value HT/HTL certification. >>> Starting HT/HTL study groups would be a good way to get people >> motivated. >>> This is the kind of thing a state society can do. Individuals can to >>> it as well, but it helps to have a group for motivation. >>> >>> Of course, that is why it has not happened - it depends too much on a >>> few super-motivated individuals. The schools don't hear of a demand >>> and so do not have programs. No programs means students never hear >>> about it. Since students don't hear about it they can't even consider >> it. A vicious circle. >>> >>> Imagine if the schools had students asking about courses leading to >>> histotech certification or at least a chance at a job that leads to >>> certification. That gets the schools attention. Junior colleges >>> especially are very interested in providing trained people to the >>> local job market. For them jobs = program justification. >>> >>> Think about it. >>> >>> Dan in Danville (a pseudonom to allow free speech) >>> >>> >>> >>> >>> >>> >>> Dear Pam: >>> >>> For a long time histologists have gotten the short end of the stick. I >> >>> believe that the current situation is a symptom of the problem and is >>> due to a history of underpayment. >>> >>> We cannot solve the problem by artificial means. This country is based >> >>> on the free market system. We must work with the system not against >>> it, but first we must clearly understand the problem: Not enough >>> techs, increasing barriers to entry into the profession, lack of >>> visibility, no central planning to fix or even research the problem. >>> >>> We need to work to bring up the wages of HTs all across the country. >>> If we do this, we will bring Histology to the attention of people in >>> education at the moment. >>> >>> We can only get out of it by concentrating on increasing the number of >> >>> facilities that are prepared to take on new graduates and spend the >>> time to get their practical skills up to speed. >>> >>> I think you are doing a valuable job bringing this situation back to >>> the surface. We need to keep the momentum up and not let it die on the >> >>> vine of this valuable forum as a forgotten conversation. So, I ask my >>> peers: What can we do to address this situation in an organized, >>> effective manner? Who in our profession is responsible for being our >> cheerleader? >>> >>> Finally, Pam, I want to say for myself that I encourage all >> conversation. >>> >>> I will never be offended by a question, and I will always try to >>> answer questions in a way that remembers there is a person behind it. >>> No flame retardant needed here. >>> >>> I make my money from placing people on a temporary and permanent >> basis. >>> >>> I am also a Histologist first and foremost and believe that patient >>> safety is number one and understaffed laboratories experience more >> mistakes. >>> >>> Keep up the good work, Pam. >>> >>> All my best, >>> >>> Anthony Williams BSc. HT >>> >>> Histotech Exchange LLC >>> >>> 19 Whitmore St. >>> >>> Lexington, VA 24450 >>> >>> T 1 (302) 383 9780 >>> >>> F 1 (540) 463 3583 >>> >>> anthony@histotechexchange.com >>> >>>> I know this is a problem that has plagued facilities for years and I >>> >>>> too have noticed a change in the past 2 years. Yes, the histology >>> >>>> programs nationwide produce a great albeit small group of talented >>> >>>> people every year but the pool of available histo techs for permanent >>> >>>> positions has shrunk even more in recent years. At the risk of being >>> >>>> "flamed" by travel companies I have to say that you are losing alot >>>> of >>> >>>> techs to travel positions. In the past 2 years of all of the histo >>> >>>> techs I have had contact with over half only want to work in >>>> permanent >>> >>>> positions the rest either want to continue as travelers or become >>> >>>> travelers. Think about it... they get a higher rate of pay, benefits >>> >>>> and living expenses paid for. For these people it is a "better deal" >>> >>>> than committing to one facility. As a matter of fact it is a "better >>> >>>> deal" than a temp/travel position in any other field outside of >>> >>>> healthcare. Facilities who take the "quick solution" of hiring travel >>> >>>> techs are contributing to the shortage. May I offer some solutions? >>>> Some >>> creative hiring strategies? >>> >>>> >>> >>>> Here are some ideas I would like to share: >>> >>>> 1. If you are using travel techs do it with a temp to perm clause - >>> >>>> but be firm. If a tech works for you as a temp make sure they are at >>> >>>> least considering converting to a permanent employee at the end of >>>> the >>> >>>> contract. If not don't extend, have your travel company send someone >>> >>>> else who would consider converting to a permanent position. And make >>> >>>> questions about their intentions part of your interview process the >>> >>>> same as you would if you were interviewing a candidate from out of >>> >>>> state for a permanent position. >>> >>>> >>> >>>> 2. Human Resources - Many of your allied health recruiters don't seem >>> >>>> to realize that histo techs don't grow on trees. So many times I see >>> >>>> facilities lose great techs because the hiring process has dragged >>>> out >>> >>>> and the candidate ends up taking a position with a facility that can >>> >>>> move faster. Stay on top of your hr people especially once you know >>> >>>> they have a histology candidate. >>> >>>> >>> >>>> 3. How about techs from Canada? There are alot of talented techs in >>> >>>> Canada that are interested in moving to the states and the process is >>> >>>> relatively easy due to NAFTA and the F1 visa. >>> >>>> >>> >>>> 4. How about techs that need sponsorship on an H-1 visa? I know alot >>> >>>> of companies shy away from this alternative because of the length of >>> >>>> time it can take to process a visa application but I think that if >>>> you >>> >>>> take a look at the time it takes to find a tech at all against the >>> >>>> time it would take to process an H-1 visa it is quickly becoming 6 of >> >>>> one >>> vs. >>> >>>> half dozen of another. I mean what difference does it make if it >>> >>>> takes up to 8 weeks to process an H-1 visa vs. 2-3 months to identify >>> >>>> a histology candidate? >>> >>>> >>> >>>> Your best bet is to get with your Human Resources department and >>> >>>> strategize, educate them on the challenges and shortages you are >> facing. >>> >>>> Discuss some of these options or others you might come up with. >>> >>>> I hope this helps!! >>> >>>> >>> >>>> Thank You! >>> >>>> >>> >>>> >>> >>>> Pam Barker >>> >>>> President >>> >>>> RELIA >>> >>>> Specialists in Allied Healthcare Recruiting >>> >>>> 5703 Red Bug Lake Road #330 >>> >>>> Winter Springs, FL 32708-4969 >>> >>>> Phone: (407)657-2027 >>> >>>> Cell: (407)353-5070 >>> >>>> FAX: (407)678-2788 >>> >>>> E-mail: relia1@earthlink.net >>> >>>> _______________________________________________ >>> >>>> Histonet mailing list >>> >>>> Histonet@lists.utsouthwestern.edu >>> >>>> >>> *http://lists.utsouthwestern.edu/mailman/listinfo/histonet*>> ts.utsou thwestern.edu/mailman/listinfo/histonet> >>> >>>> >>> >>> >>> >>> >>> >>> _______________________________________________ >>> >>> Histonet mailing list >>> >>> Histonet@lists.utsouthwestern.edu >>> >>> *http://lists.utsouthwestern.edu/mailman/listinfo/histonet*>> ts.utsou thwestern.edu/mailman/listinfo/histonet> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> >> >> >> >> >> >> This e-mail message, including any attachments, is for the sole use of >> the intended recipients and may contain privileged information. Any >> unauthorized review, use, disclosure or distribution is prohibited. If >> you are not the intended recipient, please contact the sender by e-mail >> and destroy all copies of the original message, or you may call >> PhenoPath Laboratories, Seattle, WA U.S.A. >> at (206) 374-9000. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If >> you are not the addressee intended / indicated or agent responsible for >> delivering it to the addressee, you are hereby notified that you are in >> possession of confidential and privileged information. Any dissemination, >> distribution, or copying of this e-mail is strictly prohibited. If you have >> received this message in error, please notify the sender immediately and >> delete this email from your system. >> >> >> >> PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If >> you are not the addressee intended / indicated or agent responsible for >> delivering it to the addressee, you are hereby notified that you are in >> possession of confidential and privileged information. Any dissemination, >> distribution, or copying of this e-mail is strictly prohibited. If you have >> received this message in error, please notify the sender immediately and >> delete this email from your system. >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > Judith Williams, PhD, HT(ASCP) > Research Scientist > Department of Comparative Medicine > University of Washington > Seattle, WA 98195 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Judith Williams, PhD, HT(ASCP) Research Scientist Department of Comparative Medicine University of Washington Seattle, WA 98195 From ploykasek <@t> phenopath.com Thu Apr 10 16:26:50 2008 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Thu Apr 10 16:27:00 2008 Subject: [Histonet] Surfactant antibody Message-ID: Hi all. I have just found out (to my horror) that Dako is discontinuing their Surfactant Apo A antibody clone PE-10. I have done a quick search, and can't find this clone from anyone else. Part of my angst is that I am due to go on vacation for 2 weeks starting after tomorrow. I would appreciate any help with possible suppliers. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From bdelescavage <@t> cellnetix.com Thu Apr 10 16:27:44 2008 From: bdelescavage <@t> cellnetix.com (Beth Delescavage) Date: Thu Apr 10 16:31:37 2008 Subject: [Histonet] CMV positive tissue slides and blocks Message-ID: Hi Everyone~ Aside from Newcomers Supply does anyone know where I can purchase CMV positive slides and/or blocks? Thanks in advance. Beth Delescavage, BS, HTL (ASCP) QIHC CellNetix Labs Seattle, WA DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From jodiputnam <@t> aol.com Thu Apr 10 16:38:55 2008 From: jodiputnam <@t> aol.com (jodiputnam@aol.com) Date: Thu Apr 10 16:39:04 2008 Subject: [Histonet] Salary / Temp positions In-Reply-To: <47FE6C05.1060709@bms.com> References: <5d9104a30804101012y48035d2cwefc3148107838ed9@mail.gmail.com> <05CAE76AB5D5ED409864C6DD86F13349018863FF64@pbpsflexch02.pbp.local> <47FE6C05.1060709@bms.com> Message-ID: <8CA696A68EC4F7B-494-25CF@webmail-stg-d08.sysops.aol.com> Elizabeth, You have some good points too. Everyone has I think. But I can relate to the pain and suffering of working with a tech with no knowledge of medical terminology, chemistry, bio, you name it. I've had techs make up the 20% HCI for an iron stain using ammonium hydroxide and not knowing why it didn't work. I had one tech that just passed the HT ask me how to make up 70% alcohol from 100%(200 proof) stock. She didn't know what to dilute it with. I waited for the punch line because I thought there had to be one. I was wrong. OJT is how I learned 18 years ago. I took the exam half way through and passed but the studying and time that went into it was intense. I luckily did have some chemistry, biology and anatomy courses in college so that helped. I'm concerned that techs are not being trained correctly or thoroughly due to shortages and lack of time. This is a problem when you just sit people down to embed or cut that don't even know how the process starts. I always trained from the grossing stage, explaining what the processor does (some don't realize that xylene and water don't mix.) and then moved on to embedding. Not until the tech was able to properly orient the tissue all on one level consistently did we even think about microtomy. I can't tell you the times that I had to tell people to show respect for the microtome as you can be injured in the blink of an eye. I would tell them to treat every specimen as it were your own or that of a loved one. Rushing just to get done and possibly cutting through a cancer etc. is jeopardizing the patients diagnosis and life. It saddens me when I see techs do poorly but in some cases it was the trainers fault for rushing and not helping them to understand each step and why we do what we do. I felt so sorry for a girl that eventually got fired. She was an average tech but chunked out alot of blocks. She always was treated poorly when she did special stains and they didn't work. The fact is she didn't KNOW it didn't work. I asked why aren't you showing the techs what a positive control looks like and how to scope things etc,. and was told that there just isn't enough time. Long story short, I was OJT and that worked for me. Some of the best techs that I have worked with didn't have the HT. Some people need the college courses or program when some but not all labs are not training thoroughly.? Sorry for the lengthy message but as most, I tend to get very passionate about this subject.? Thanks for listening, Jodi -----Original Message----- From: Elizabeth M Heimrich To: Judy Collins Cc: histonet@lists.utsouthwestern.edu Sent: Thu, 10 Apr 2008 2:35 pm Subject: Re: [Histonet] Salary / Temp positions I took that course!! It is a very informative independant learning exercise, but they have a set of prerequisites such as biology and chemistry. Thenin lies the problem for a lot of OJT Histotechs. They have the experience, but they don't have the college courses. We have a problem, so now what do we do?!? Personally I think the techs should have the bio and chem background because a lot of techs who are not science savy have done some harmful mistakes. For example, I have seen a lab aid dump unneutralized formalin waste down the drain. Another tech thought it was OK to store acids and bases in the same cabinet, and even another person with no chem background made a batch of acid alcohol with 10% NBF!! My slides that day looked like garbage!! No wonder the safety cops have cracked down!!! Just my 2 cents,? Beth? ? Judy Collins wrote:? ? >What about the nontraditional learning programs such as Indiana University's? Our laboratory has helped 5 prospective techs through the past few years to become histotechnicians through their program. The program is 10 months long at a very reasonable cost and the candidates are eligible to sit for the ASCP at the end of the program. I believe there are some other similar programs as well.? >? >Judy Collins? >Palm Beach Pathology? >? >-----Original Message-----? >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy DuBois? >Sent: Thursday, April 10, 2008 1:13 PM? >To: histonet@lists.utsouthwestern.edu? >Subject: [Histonet] Salary / Temp positions? >? >If we think about the cost of 2-4 years of college to obtain a degree in? >order to qualify for the Histology test, most of us would be in debt when? >finished. Then look at our salary and you can see how the requirements just? >aren't supported by the salary. Most of the students will come out of? >college owing on student loans. The salaries they would receive as new? >histotech would allow them to pay off their student loanswhile maintaining a? >decent living (at least here in CA).? >With both my sons in college (using student loans) we had to take a serious? >look at the final amount they will owe when the graduate and compare it to? >what they would be earning.? >I am not sure what the solution is. Any ideas?? >? >Cindy? >_______________________________________________? >Histonet mailing list? >Histonet@lists.utsouthwestern.edu? >http://lists.utsouthwestern.edu/mailman/listinfo/histonet? >? >_______________________________________________? >Histonet mailing list? >Histonet@lists.utsouthwestern.edu? >http://lists.utsouthwestern.edu/mailman/listinfo/histonet? > >? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rick.Garnhart <@t> memorialhealthsystem.com Thu Apr 10 17:24:45 2008 From: Rick.Garnhart <@t> memorialhealthsystem.com (Rick.Garnhart@memorialhealthsystem.com) Date: Thu Apr 10 17:26:52 2008 Subject: [Histonet] Rick Garnhart/Histology/MEMHOSPCS is out of the office. Message-ID: I will be out of the office starting 04/10/2008 and will not return until 04/14/2008. I will respond to your message when I return. From Bekki.Haggerty <@t> cptc.edu Thu Apr 10 18:05:33 2008 From: Bekki.Haggerty <@t> cptc.edu (Haggerty, Bekki) Date: Thu Apr 10 18:00:46 2008 Subject: [Histonet] vacancies Message-ID: <16E44253DE09FA4FBFD337F5C7CEC2482890D4@ms1726.cptc.edu> Let me introduce myself. I am Bekki Haggerty the instructor for the Clover Park Technical College Histology Program. I have been a certified Ht for 15 years and graduated from the school in Seattle in 1993. We are in our 3rd quarter of instruction and the students are already well trained to cut, embed and have run a battery of special stains. I currently have 11 students who are ambitious and enthusiastic towards their career choice. We will be entering a unit of IHC this quarter also. Thses students are scheduled to hit the work force at the end of summer 2008. Each and every one of them will be fantastic techs!!!! Look for their arrival into the histology world shortly. Rebecca Haggerty HT ASCP Clover Park Technical College Histology Technician Instructor Bekki.Haggerty@cptc.edu From godsgalnow <@t> aol.com Thu Apr 10 18:37:21 2008 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Thu Apr 10 18:37:35 2008 Subject: [Histonet] CMV positive tissue slides and blocks In-Reply-To: References: Message-ID: <8CA697AF4F8A50F-BC-2EC7@webmail-db08.sysops.aol.com> You can get control slides from BBC Biochemical.? I just ordered some CMV controls from them myself.? WHen you call ask for Adrian, he will help you, or Tim. Roxanne -----Original Message----- From: Beth Delescavage To: histonet Sent: Thu, 10 Apr 2008 5:27 pm Subject: [Histonet] CMV positive tissue slides and blocks Hi Everyone~ Aside from Newcomers Supply does anyone know where I can purchase CMV positive slides and/or blocks? Thanks in advance. Beth Delescavage, BS, HTL (ASCP) QIHC CellNetix Labs Seattle, WA DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mmarti <@t> cmrb.eu Fri Apr 11 03:33:58 2008 From: mmarti <@t> cmrb.eu (=?iso-8859-1?Q?Marti_Gaudes=2C_Merc=E8?=) Date: Fri Apr 11 03:35:01 2008 Subject: [Histonet] Antigenecity lost with decalcification Message-ID: Hello. We've done some fluorescent IHC against GFP, but they didn't work. We believe that the GFP antigen(s) are lost during the decalcification process (92% EtOH 70% + 8% Nitric acid for 2 days) but, for example, anti-phospho-Histone3 is working fine. Do you know any decalcification protocol that doesn't interfere with the antigenicity? Thank you! Merc? Mart? Gaudes Cap de Servei Histology and Bioimaging Centre de Medicina Regenerativa de Barcelona (CMR[B]) Dr. Aiguader, 88 7ena planta 08003 Barcelona mmarti@cmrb.eu Tel: +34 93 316 03 51 Fax: +34 93 224 10 83 From lpwenk <@t> sbcglobal.net Fri Apr 11 07:24:42 2008 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Fri Apr 11 07:24:53 2008 Subject: [Histonet] Salary / Temp positions In-Reply-To: <05CAE76AB5D5ED409864C6DD86F13349018863FF6D@pbpsflexch02.pbp.local> Message-ID: <000801c89bcf$05a53db0$0202a8c0@HPPav2> There are 2 routes to take the ASCP HT exam. Route 1: Successful completion of a NAACLS accredited Histotechnician program within the last 5 years prior to the date of application for examination; or Route 2: Associate degree or at least 60 semester hours (90 quarter hours) of academic credit from a regionally accredited college/university, with a combination of 12 semester hours (18 quarter hours) of biology and chemistry, AND one year full time acceptable experience in histopathology in the U.S., Canada or a CAP/The Joint Commission (JCAHO)/AABB accredited laboratory within the last ten years under the supervision of a pathologist (certified by the American Board of Pathology in Anatomic Pathology), or an appropriately board certified medical scientist. So, if you notice, Route 2 is the on-the-job training route (OJT) that DOES require an associate degree or 60 semester hours. However, Route 1 is through a NAACLS HT program. ASCP does not require any degree or classes to take the HT exam via this route. However, if you were to look at the NAACLS requirements for accreditation of a HT program, the minimum requirement is completion of high school and the person must have taken (minimum) 1 biology, 1 chemistry and 1 math course, sometime in high school or college, before entering the HT program. Now, every HT NAACLS program can set their own standards, as long as they meet the NAACLS requirements or are higher. For example, my HT program requires an associate degree, with anatomy, physiology, microbiology, 2 chemistry, and intermediate college algebra as my minimum. I am affiliated with several community colleges, so the students earn 12-15 college credits while attending my program, which show up on the transcript along with the grade they earn in my program, which gets figured into their GPA. For these affiliated colleges, the associate degree in HT also requires the students to take technical writing, medical terminology, medical law/ethics. If I don't fill my class with students from my affiliates, then I go to people with associate degrees from other institutions who meet my minimum. Other NAACLS HT program will take people with HS diploma (with the bio/chem/math), or some college (with the bio/chem/math), or a full associate degree (with the bio/chem/math). Some will require additional classes. So, in the case of Indiana U, I believe they will take someone with a HS diploma, with a bio, chem and math classes from HS, and accept them into the program. Once they successfully complete the IU program, they can sit for the ASCP HT exam via Route 1. There are several HT distance learning programs out there, that you might want to check into for yourself or for other people in your lab that want to take the ASCP HT exam, and either don't qualify now, or need some additional help studying. I don't know the "minimum requirements" for these NAACLS programs. You would have to check them out yourself. Indiana University School of Medicine Coleman Hall, Room 322 1140 West Michigan Street Indianapolis, IN 46202-5113 Ms. Debra Wood BS, HT(ASCP) (317) 491 6410 Harford Community College Allied Health Department 401 Thomas Run Road Bel Air, MD 21014 Ms. Anne Marie McCauley, RN, BS 410) 836-4389 University of North Dakota School of Medicine & Health Sciences - Dept. of Pathology PO Box 9037 Grand Forks, ND 58202 Ms. Ruth Paur, MS, MT(ASCP), CLS(NCA) 701) 777-2651 Columbus State Community College 550 East Spring Street Columbus, OH 43215-0965 Ms. Peggy Mayo, MEd, MLT(ASCP) 614) 287-2608 And there is one in Florida, but I don't remember which of the 2 NAACLS HT Florida program has the distance learning. Sorry. Maybe someone can chime in and let us know. Peggy A. Wenk, HTL(ASCP)SLS Schools of Histotechnology William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Judy Collins Sent: Thursday, April 10, 2008 3:03 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Salary / Temp positions I don't think that is correct. Our current student does not have an AA, will finish the program in May and will be taking the ASCP. I think they were talking about requiring an AA but that never was instituted officially. Judy Collins -----Original Message----- From: Martin, Gary [mailto:gmartin@marshallmedical.org] Sent: Thursday, April 10, 2008 2:18 PM To: Judy Collins Subject: RE: [Histonet] Salary / Temp positions I believe you have to have the minium of an AA Gary -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Judy Collins Sent: Thursday, April 10, 2008 10:37 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Salary / Temp positions What about the nontraditional learning programs such as Indiana University's? Our laboratory has helped 5 prospective techs through the past few years to become histotechnicians through their program. The program is 10 months long at a very reasonable cost and the candidates are eligible to sit for the ASCP at the end of the program. I believe there are some other similar programs as well. Judy Collins Palm Beach Pathology -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy DuBois Sent: Thursday, April 10, 2008 1:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Salary / Temp positions If we think about the cost of 2-4 years of college to obtain a degree in order to qualify for the Histology test, most of us would be in debt when finished. Then look at our salary and you can see how the requirements just aren't supported by the salary. Most of the students will come out of college owing on student loans. The salaries they would receive as new histotech would allow them to pay off their student loanswhile maintaining a decent living (at least here in CA). With both my sons in college (using student loans) we had to take a serious look at the final amount they will owe when the graduate and compare it to what they would be earning. I am not sure what the solution is. Any ideas? Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Apr 11 07:32:36 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Apr 11 07:32:44 2008 Subject: [Histonet] workload per person In-Reply-To: Message-ID: <308638.48993.qm@web65715.mail.ac4.yahoo.com> The embedding median value (176 labs) = 60 blocks /hour. This amount depends on personal dexterity, not on training status. There are some HTs that after 20 years can embed only 25-30 blocks/hour and I have seen one very fast one doing an average of 176 blocks/hour. Ren? J. MARY T HODGES wrote: hello all, Can any one give me some guide lines on how many blocks someone should embed in the morning also how many blocks. Please take in consideration beginners,intermediate level and senior level techs Thanks, Tere Hodges. _________________________________________________________________ More immediate than e-mail? Get instant access with Windows Live Messenger. http://www.windowslive.com/messenger/overview.html?ocid=TXT_TAGLM_WL_Refresh_instantaccess_042008_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From Lynn.Burton <@t> Illinois.gov Fri Apr 11 08:05:57 2008 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Fri Apr 11 08:07:30 2008 Subject: [Histonet] Histonet Vacancies---DSI !! References: Message-ID: Would you please share the link to that website and game again. I missed it the first go round. Lynn Burton Animal Disease Lab Galesburg,Il ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Judith L. Williams Sent: Thu 4/10/2008 12:41 PM To: Cheri Miller Cc: Histonet@lists.utsouthwestern.edu; Jason.Kruse@cptc.edu Subject: RE: [Histonet] Histonet Vacancies---DSI !! HI - I am Judith Williams, the creator of DSI!!! along with Jason Kruse. I was the instructor at Clover Park Tech College last year and then I took another job at Univ of WAshington. I designed the idea and text along with Jason, one of their computer experts at the college. He did a fantastic job of making it interesting! He came to the lab and took photos and I explained each step- he took notes then made the cartoons and applied it. He and I worked on it, editing and tweeking it - he is so clever! I really want him to get a lot of credit for it. Isn't it wonderful! He and I are going to make CD's of it to send out for advertising histotechnology. Will let you know about when they are going to be ready. Glad you all enjoyed it. I am a huge CSI fan - so naturally we did that twist! cheers to all histoworld!!! We need to sell this career - its great! Judith Williams, PhD, HT(ASCP) Department of Comparative Medicine University of Washington Seattle, WA On Thu, 10 Apr 2008, Cheri Miller wrote: > I agree, its very clever! I have saved it to share with my department! > Cheryl Miller HT (ASCP) > Histology Supervisor > Physicians Laboratory,P.C. > Omaha, Ne. > 402 738 5052 > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim > Sent: Thursday, April 10, 2008 10:37 AM > To: Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Histonet Vacancies > > > Patty, Thanks for the link to the new program. And the histology "DSI" > game on the site is brilliant!! > > > Tim Morken > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti > Loykasek > Sent: Thursday, April 10, 2008 7:41 AM > To: Dan Dan; Histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Histonet Vacancies > > Well said, Dan. I do have one small caveat, there is a new school in the > Northwest, Tacoma WA area. It is at Clover Park Technical College. Check > it out at www.cptc.edu. The area histotechs have been quite supportive, > and I think the program is off to a great start. > > > Patti Loykasek BS, HTL, QIHC > PhenoPath Laboratories > Seattle, WA > > > > >> I'll add my two-cents worth. It's hard to believe that after 25 years >> in this business that the histotech "shortage" situation is pretty >> much the same as it was long ago. There have certainly been >> improvements in education and training of people in histology, but for > >> the most part (99%??) people just fall into histology rather than pick > >> it at some point in school. In fact, it is a very rare day when I meet > >> someone who went to school for histotechnology, At this date there is >> still only one school on the west coast, and it is not the same school > that existed for a while in Seattle! >> The new one is in southern california. I looked into starting a >> histotechnology course here in the SF Bay Area, but it takes a long >> while with lots of justification and scrounging for equipment and if I > >> wanted to do it full time the pay would be half as much as I make now. > >> I could do it part time but then I'd be working 60-70 hours a week >> rather than 50 I do now. For the time being I give presentations to >> the local junior colleges. I may expand that to high schools. I know >> they are interested in getting speakers, but finding the time... >> >> I work in the biotech field as an IHC lab manger. We don't require >> HT/HTL/QIHC (though would love to have them!)but it is still very >> difficult to get experienced histotechs and nearly impossible to get >> experienced IHC techs around here. Most of the people I interview are >> from the research field who may have done some IHC at some point. And >> the ones who have good IHC experience are making very high salaries >> that our company gags at matching. It is not possible to recruit out >> of the Bay Area because the cost of housing is so high that no one >> that is not already living here can afford to move here. Even getting >> someone from southern cal is difficult - justifiying relocation >> expenses is difficult. It is kind of a joke that the experienced IHC >> techs know each other and spend their careers trading places at the >> varioius companies. Anthony is quite right that it takes a lot of > effort to educate HR and upper management about the need for higher pay. >> They really have a hard time justifying the same pay for a >> technologist that a new PhD will also make. >> >> On top of that we have loads of (legal) immigrants from Asia here and >> they are quite willing to work for lower pay to get a start. It is >> very common to have PhD level people, and even immigrant MD's (who >> can't get board certified here, mainly due to lanquage difficulties) > working as lab techs. >> Who is going to hire a AA/BS histotech when they can get a PhD who is >> happy to do the work and able to contribute to R&D at a higher level >> than most techs? >> >> There is a huge disparity between what hospitals and private >> diagnostic labs pay. The local Kaiser labs and Univeristy hospital >> labs pay in the $35 per hour range for basic histology. Senior IHC >> techs are in the $45 range. Those places have unions which is why the >> pay is so high. On the other hand, private labs will pay >> experieinced,certified people almost that much, but new, inexperienced > >> people may be paid $15-20/hr and the lab is not willing to help them >> get to their HT/HTL because they don't want to pay them more, and >> could lose them to the hospitals once trained. This is not speculation > >> but things I have had told to me first hand from techs who work in > these places. >> >> It is a little harder now for histotechs to be trained on the job due >> to the AA requirement. Most OJT people I have met were working in the >> hospital at some other job - lab assisant, dishwasher, etc before >> getting (accidently) into histology. A person working on an AA is >> probably not working in those jobs and probably does not even know a > histology lab exists. >> >> Obviously the need is to get exposure for histotechnology. The NSH >> makes brochures and videos available and does a career day for high >> schools students at the national meeting every year (I've helped at >> those). This is a good foundation. But it will really take every state > >> society and every interested histotech to do SOMETHING to promote the >> field. Science teachers at high schools are very interested in having >> working people come in to talk about a field. If someone in every city > >> could do that it would go a long way towards promoting the field. >> Maybe the key is to have NSH come up with a standardised presentation >> that anyone can give along with some props to make it a tactile > experience. >> >> But, after the promotion you have to realize that it takes a huge >> amount of initiative on the students part to follow through. They have > >> to start work on, or finish, an AA /BS degree, make contact with a lab > >> that is interested in training people, get the position, do on-the-job > >> (maybe at the same time as school) and THEN work on learning the >> matieral for an HT/HTL pretty much alone. Their facing a 3 to 4 year >> process. Longer if they get a bachelors degree. Key is finding labs to > training that value HT/HTL certification. >> Starting HT/HTL study groups would be a good way to get people > motivated. >> This is the kind of thing a state society can do. Individuals can to >> it as well, but it helps to have a group for motivation. >> >> Of course, that is why it has not happened - it depends too much on a >> few super-motivated individuals. The schools don't hear of a demand >> and so do not have programs. No programs means students never hear >> about it. Since students don't hear about it they can't even consider > it. A vicious circle. >> >> Imagine if the schools had students asking about courses leading to >> histotech certification or at least a chance at a job that leads to >> certification. That gets the schools attention. Junior colleges >> especially are very interested in providing trained people to the >> local job market. For them jobs = program justification. >> >> Think about it. >> >> Dan in Danville (a pseudonom to allow free speech) >> >> >> >> >> >> >> Dear Pam: >> >> For a long time histologists have gotten the short end of the stick. I > >> believe that the current situation is a symptom of the problem and is >> due to a history of underpayment. >> >> We cannot solve the problem by artificial means. This country is based > >> on the free market system. We must work with the system not against >> it, but first we must clearly understand the problem: Not enough >> techs, increasing barriers to entry into the profession, lack of >> visibility, no central planning to fix or even research the problem. >> >> We need to work to bring up the wages of HTs all across the country. >> If we do this, we will bring Histology to the attention of people in >> education at the moment. >> >> We can only get out of it by concentrating on increasing the number of > >> facilities that are prepared to take on new graduates and spend the >> time to get their practical skills up to speed. >> >> I think you are doing a valuable job bringing this situation back to >> the surface. We need to keep the momentum up and not let it die on the > >> vine of this valuable forum as a forgotten conversation. So, I ask my >> peers: What can we do to address this situation in an organized, >> effective manner? Who in our profession is responsible for being our > cheerleader? >> >> Finally, Pam, I want to say for myself that I encourage all > conversation. >> >> I will never be offended by a question, and I will always try to >> answer questions in a way that remembers there is a person behind it. >> No flame retardant needed here. >> >> I make my money from placing people on a temporary and permanent > basis. >> >> I am also a Histologist first and foremost and believe that patient >> safety is number one and understaffed laboratories experience more > mistakes. >> >> Keep up the good work, Pam. >> >> All my best, >> >> Anthony Williams BSc. HT >> >> Histotech Exchange LLC >> >> 19 Whitmore St. >> >> Lexington, VA 24450 >> >> T 1 (302) 383 9780 >> >> F 1 (540) 463 3583 >> >> anthony@histotechexchange.com >> >>> I know this is a problem that has plagued facilities for years and I >> >>> too have noticed a change in the past 2 years. Yes, the histology >> >>> programs nationwide produce a great albeit small group of talented >> >>> people every year but the pool of available histo techs for permanent >> >>> positions has shrunk even more in recent years. At the risk of being >> >>> "flamed" by travel companies I have to say that you are losing alot >>> of >> >>> techs to travel positions. In the past 2 years of all of the histo >> >>> techs I have had contact with over half only want to work in >>> permanent >> >>> positions the rest either want to continue as travelers or become >> >>> travelers. Think about it... they get a higher rate of pay, benefits >> >>> and living expenses paid for. For these people it is a "better deal" >> >>> than committing to one facility. As a matter of fact it is a "better >> >>> deal" than a temp/travel position in any other field outside of >> >>> healthcare. Facilities who take the "quick solution" of hiring travel >> >>> techs are contributing to the shortage. May I offer some solutions? >>> Some >> creative hiring strategies? >> >>> >> >>> Here are some ideas I would like to share: >> >>> 1. If you are using travel techs do it with a temp to perm clause - >> >>> but be firm. If a tech works for you as a temp make sure they are at >> >>> least considering converting to a permanent employee at the end of >>> the >> >>> contract. If not don't extend, have your travel company send someone >> >>> else who would consider converting to a permanent position. And make >> >>> questions about their intentions part of your interview process the >> >>> same as you would if you were interviewing a candidate from out of >> >>> state for a permanent position. >> >>> >> >>> 2. Human Resources - Many of your allied health recruiters don't seem >> >>> to realize that histo techs don't grow on trees. So many times I see >> >>> facilities lose great techs because the hiring process has dragged >>> out >> >>> and the candidate ends up taking a position with a facility that can >> >>> move faster. Stay on top of your hr people especially once you know >> >>> they have a histology candidate. >> >>> >> >>> 3. How about techs from Canada? There are alot of talented techs in >> >>> Canada that are interested in moving to the states and the process is >> >>> relatively easy due to NAFTA and the F1 visa. >> >>> >> >>> 4. How about techs that need sponsorship on an H-1 visa? I know alot >> >>> of companies shy away from this alternative because of the length of >> >>> time it can take to process a visa application but I think that if >>> you >> >>> take a look at the time it takes to find a tech at all against the >> >>> time it would take to process an H-1 visa it is quickly becoming 6 of > >>> one >> vs. >> >>> half dozen of another. I mean what difference does it make if it >> >>> takes up to 8 weeks to process an H-1 visa vs. 2-3 months to identify >> >>> a histology candidate? >> >>> >> >>> Your best bet is to get with your Human Resources department and >> >>> strategize, educate them on the challenges and shortages you are > facing. >> >>> Discuss some of these options or others you might come up with. >> >>> I hope this helps!! >> >>> >> >>> Thank You! >> >>> >> >>> >> >>> Pam Barker >> >>> President >> >>> RELIA >> >>> Specialists in Allied Healthcare Recruiting >> >>> 5703 Red Bug Lake Road #330 >> >>> Winter Springs, FL 32708-4969 >> >>> Phone: (407)657-2027 >> >>> Cell: (407)353-5070 >> >>> FAX: (407)678-2788 >> >>> E-mail: relia1@earthlink.net >> >>> _______________________________________________ >> >>> Histonet mailing list >> >>> Histonet@lists.utsouthwestern.edu >> >>> >> *http://lists.utsouthwestern.edu/mailman/listinfo/histonet*> ts.utsou thwestern.edu/mailman/listinfo/histonet> >> >>> >> >> >> >> >> >> _______________________________________________ >> >> Histonet mailing list >> >> Histonet@lists.utsouthwestern.edu >> >> *http://lists.utsouthwestern.edu/mailman/listinfo/histonet*> ts.utsou thwestern.edu/mailman/listinfo/histonet> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > > > > This e-mail message, including any attachments, is for the sole use of > the intended recipients and may contain privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. If > you are not the intended recipient, please contact the sender by e-mail > and destroy all copies of the original message, or you may call > PhenoPath Laboratories, Seattle, WA U.S.A. > at (206) 374-9000. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If > you are not the addressee intended / indicated or agent responsible for > delivering it to the addressee, you are hereby notified that you are in > possession of confidential and privileged information. Any dissemination, > distribution, or copying of this e-mail is strictly prohibited. If you have > received this message in error, please notify the sender immediately and > delete this email from your system. > > > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Judith Williams, PhD, HT(ASCP) Research Scientist Department of Comparative Medicine University of Washington Seattle, WA 98195 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Apr 11 08:54:39 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Apr 11 08:54:42 2008 Subject: [Histonet] microwave processors In-Reply-To: <47FCDFE9.A3DC.00A0.0@tvmdl.tamu.edu> Message-ID: <612291.38080.qm@web65713.mail.ac4.yahoo.com> IF you have a turn around time issue, it is worth considering the MW option, BUT if you are not pressured by the TAT it is NOT worth it. Consider the following: NO matter how fast you process your tissues, you will need EXACTLY the same time PRE and POST processing to take care of your 100 cassettes. You probably will cut in half your processing time (since you are aiming at 3 hours processing time), so your total TAT will be about 80% of your present TAT. And NO, a modified lab MW oven will not produce the same results. If you want I can send you a study I published on the subject. Ren? J. Stephanie Weaver wrote: __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From Luis.Chiriboga <@t> med.nyu.edu Fri Apr 11 10:14:25 2008 From: Luis.Chiriboga <@t> med.nyu.edu (Luis Chiriboga) Date: Fri Apr 11 09:13:58 2008 Subject: [Histonet] Histology vacancies In-Reply-To: <842113.49682.qm@web31305.mail.mud.yahoo.com> Message-ID: Hi all I'm not sure how the law is set-up in CA but it is a complete mess here in New York.......Our law, just passed in 2006 requires all "clinical laboratory" personnel to be licensed. Briefly, the law created a generalist license, allowing anyone who meets the educational and test criteria to practice in a clinical laboratory. However, there is no distinction between specialties, and no mention of histology. In fact the educational curricula proposed only requires 1 semester course in histology (microscopic) in order to be licensed. IN addition, anyone wishing to come to New York to practice in histology now has to meet the educational requirements (specific course work) and take the generalist test (covers all areas but is mostly CP.....from chemistry to blood banking) regardless of years in practice, certifications or degrees. I believe that there are a few other, if not several, states considering licensing legislation. I highly recommend that histology people get involved..... get your state society to represent you in this process. Otherwise, you may end up far worse off than you already may be....... Luis Chiriboga PhD., HT (ASCP) QIHC New York University School of Medicine NYU Cancer Institute IHC Core Facility Bellevue Hospital Center Department of Pathology 4W27 27th Street & First Avenue New York, N.Y. 10016 V (212)562-4667 F (212)263-2041 Luis.Chiriboga@med.nyu.edu Vice President New York State Histotechnological Society http://www.nyhisto.org/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Akemi Allison-Tacha Sent: Thursday, April 10, 2008 2:35 PM To: Histonet Subject: [Histonet] Histology vacancies Hello Dan in Danville, Having worked and lived in the SF Bay Area for a number of years, and in Danville too, I know exactly what you are talking about! I agree with everything you and Patti stated. You may or may not be aware of it, but Governor Arnold Schwarzenegger has mandated that ALL laboratory staff working in health care, be certified by 2009, or was it 2010. This includes histologists. I know of a number of histologists that are not certified in CA, and this is going to cause a huge problem, as well as further shortages in histology laboratories. NY has been dealing with this issue too. Akemi Allison-Tacha, BS, HT(ASCP)HTL Client Services Manager PhenoPath laboratories 551 North 34th Street, Suite 100 Seattle, WA 98103-8675 Work: (206) 374-9000 E-Mail: akemiat3377@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jbirkner <@t> colabserv.com Fri Apr 11 09:13:53 2008 From: jbirkner <@t> colabserv.com (Jeff Birkner) Date: Fri Apr 11 09:14:05 2008 Subject: [Histonet] microwave processors In-Reply-To: <612291.38080.qm@web65713.mail.ac4.yahoo.com> Message-ID: What Rene has mentioned is true. However, we have been able to cut our reagent and paraffin usage by 80% which is a huge cost savings. We have also been able to do away with formalin and xylene, so no more costly monitoring! Jeff Birkner, CT(ASCP) Pathology Section Manager Collaborative Laboratory Services, LLC 1005 Pennsylvania Ave. Ottumwa, IA 52501 641-684-4621 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, April 11, 2008 8:55 AM To: Stephanie Weaver; histonet post Subject: Re: [Histonet] microwave processors IF you have a turn around time issue, it is worth considering the MW option, BUT if you are not pressured by the TAT it is NOT worth it. Consider the following: NO matter how fast you process your tissues, you will need EXACTLY the same time PRE and POST processing to take care of your 100 cassettes. You probably will cut in half your processing time (since you are aiming at 3 hours processing time), so your total TAT will be about 80% of your present TAT. And NO, a modified lab MW oven will not produce the same results. If you want I can send you a study I published on the subject. Ren? J. Stephanie Weaver wrote: __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMcArdle <@t> ebsciences.com Fri Apr 11 09:26:40 2008 From: PMcArdle <@t> ebsciences.com (Phil McArdle) Date: Fri Apr 11 09:26:54 2008 Subject: [Histonet] microwave processors In-Reply-To: References: Message-ID: <47FF7520.9030801@ebsciences.com> Here's a quick vendor response: I agree with Jeff's comments; the "health-and safety" aspects of reducing or eliminating formalin and xylene use results in many benefits, and reduction of reagent consumption is a win-win. Further, I believe that a laboratory microwave processor, especially one that is not limited to a single protocol or proprietary reagents, allows flexibility in the lab that can't easily be quantified via TAT alone. And finally, I hear comments all the time like "the techs always know the microwaved samples - the blocks cut better" and pathologists mention improved staining or morphology. Given these benefits, I think it's reasonable to conclude pre- and post-processing TAT might actually be improved. On another note, I will be leaving EBS on April 13. Anyone wishing to contact me may do so via my personal e-mail: mcardlepm@gmail.com. Keep up the great work, everyone! Phil McArdle -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Mobile: 860.597.6796 Fax: 860.653.0422 You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it, and it was probably my mistake anyway. So please do the right thing and make this e-mail go away. Thank you. Jeff Birkner wrote: > What Rene has mentioned is true. However, we have been able to cut our reagent and paraffin usage by 80% which is a huge cost savings. We have also been able to do away with formalin and xylene, so no more costly monitoring! > > Jeff Birkner, CT(ASCP) > Pathology Section Manager > Collaborative Laboratory Services, LLC > 1005 Pennsylvania Ave. > Ottumwa, IA 52501 > 641-684-4621 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Friday, April 11, 2008 8:55 AM > To: Stephanie Weaver; histonet post > Subject: Re: [Histonet] microwave processors > > IF you have a turn around time issue, it is worth considering the MW option, BUT if you are not pressured by the TAT it is NOT worth it. > Consider the following: NO matter how fast you process your tissues, you will need EXACTLY the same time PRE and POST processing to take care of your 100 cassettes. > You probably will cut in half your processing time (since you are aiming at 3 hours processing time), so your total TAT will be about 80% of your present TAT. > And NO, a modified lab MW oven will not produce the same results. > If you want I can send you a study I published on the subject. > Ren? J. > > Stephanie Weaver wrote: > > > > > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From PMcArdle <@t> ebsciences.com Fri Apr 11 09:29:21 2008 From: PMcArdle <@t> ebsciences.com (Phil McArdle) Date: Fri Apr 11 09:29:26 2008 Subject: [Histonet] Errata correction In-Reply-To: References: Message-ID: <47FF75C1.6030608@ebsciences.com> Errata correction: I will be leaving EBS on April 18, not 13. Sorry for the confusion! Phil McArdle -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Mobile: 860.597.6796 Fax: 860.653.0422 You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it, and it was probably my mistake anyway. So please do the right thing and make this e-mail go away. Thank you. Jeff Birkner wrote: > What Rene has mentioned is true. However, we have been able to cut our reagent and paraffin usage by 80% which is a huge cost savings. We have also been able to do away with formalin and xylene, so no more costly monitoring! > > Jeff Birkner, CT(ASCP) > Pathology Section Manager > Collaborative Laboratory Services, LLC > 1005 Pennsylvania Ave. > Ottumwa, IA 52501 > 641-684-4621 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Friday, April 11, 2008 8:55 AM > To: Stephanie Weaver; histonet post > Subject: Re: [Histonet] microwave processors > > IF you have a turn around time issue, it is worth considering the MW option, BUT if you are not pressured by the TAT it is NOT worth it. > Consider the following: NO matter how fast you process your tissues, you will need EXACTLY the same time PRE and POST processing to take care of your 100 cassettes. > You probably will cut in half your processing time (since you are aiming at 3 hours processing time), so your total TAT will be about 80% of your present TAT. > And NO, a modified lab MW oven will not produce the same results. > If you want I can send you a study I published on the subject. > Ren? J. > > Stephanie Weaver wrote: > > > > > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From JGREWE <@t> OhioHealth.com Fri Apr 11 09:02:06 2008 From: JGREWE <@t> OhioHealth.com (JGREWE@OhioHealth.com) Date: Fri Apr 11 09:31:16 2008 Subject: [Histonet] Jacquelyn Grewe/Staff/OhioHealth is out of the office . Message-ID: I will be out of the office starting 04/11/2008 and will not return until 04/21/2008. From akbitting <@t> geisinger.edu Fri Apr 11 09:55:31 2008 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Fri Apr 11 09:55:48 2008 Subject: [Histonet] Decalcifiers Message-ID: <47FF43A3.2B7F.00C9.0@geisinger.edu> We have been using the same commercial decalcifier for years and its not been as effective lately. We use it for our bone and bone marrow specimens. So I am shopping for a replacement. I'm just wondering if anyone has a favorite all-purpose decalcifier that they purchase? IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From info <@t> hphisto.com Fri Apr 11 10:01:36 2008 From: info <@t> hphisto.com (info@hphisto.com) Date: Fri Apr 11 10:01:44 2008 Subject: [Histonet] Immuno control blocks Message-ID: <5874522.181281207926096679.JavaMail.servlet@perfora> Good morning: My current assignment has me preparing microarray control blocks for IHC. Need feedback to this question: If certain microarray are optimized for a particular antibody, is it acceptable to the?pathologists?and CAP to ignore background staining in non-optimized tissues within the microarray? I know what I think, but I need more than my opinion. What's happening in this area at you respective labs? Thanks in advance -- Bill O'Donnell HT (ASCP) QIHC High Performance Histology Services LLC www.hphisto.com From gayle.callis <@t> bresnan.net Fri Apr 11 10:12:19 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Fri Apr 11 10:12:18 2008 Subject: [Histonet] Decalcifiers Message-ID: <001a01c89be6$6f830520$6401a8c0@DHXTS541> Angela, Interesting that a commercial decalcifier has been changed. You did not say WHICH decalcifying agent you are using in order for someone to make a recommendation for a similar product and continuity for your decalcification procedures. The MSDS should provide this information, or even label. Most decalcifiers can be considered all purpose, but if you want speed - then a HCl based fluid, or if you like gentler for IHC, a formic acid decalcifier? Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT From gu.lang <@t> gmx.at Fri Apr 11 10:19:02 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Apr 11 10:19:12 2008 Subject: [Histonet] Alcianblue stain - picture Message-ID: <002a01c89be7$609fb890$eeeea8c0@dielangs.at> Hi dear listmembers, We struggle with the "right" image of a good alcianblue stain of skin with lupus. We use a commercial 1% alcianblue, pH 2,5 (10 min incubation, wash in aqua dest., 5 min nuclear fast red, wash, dehydrate, clear, coverslip). We switched just two months ago from the automated Nexes stainer to the (good old) manual method. Now my pathologists complain about "too much blue" in the corium. I say, there must be a staining of the hyaluronic acid and other acid mucosubstances surrounding the collagen fibers. They say, they only want to see the mucin stained, that is typical for the lupus, in intense blue and the surrounding should be colourless. So, can somebody provide me with a picture of a good alcianblue stain of skin with lupus? - please. Or give me any helpful hint. Thanks in advance Gudrun Lang From mwich <@t> 7thwavelabs.com Fri Apr 11 10:20:45 2008 From: mwich <@t> 7thwavelabs.com (Michele Wich) Date: Fri Apr 11 10:20:54 2008 Subject: [Histonet] Ki-67 for mouse Message-ID: <2264717ADC396742A0FF0AAB674F9A0D486C46@7THWAVE-SERVER.7thwave.local> Can someone recommend a good Ki-67 antibody for use on mouse tissue? Thanks. This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer From portera <@t> msu.edu Fri Apr 11 10:23:47 2008 From: portera <@t> msu.edu (Amy Porter) Date: Fri Apr 11 10:25:34 2008 Subject: [Histonet] Ki-67 for mouse References: <2264717ADC396742A0FF0AAB674F9A0D486C46@7THWAVE-SERVER.7thwave.local> Message-ID: <000f01c89be8$0961c720$8e7a0923@histolab> Abcam - rabbit anti-Ki67 Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 355-6475 ext 1480 Fax: (517) 432-1368 Email: portera@msu.edu Web: www.humanpathology.msu.edu ----- Original Message ----- From: "Michele Wich" To: Sent: Friday, April 11, 2008 11:20 AM Subject: [Histonet] Ki-67 for mouse Can someone recommend a good Ki-67 antibody for use on mouse tissue? Thanks. This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Fri Apr 11 10:34:23 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Fri Apr 11 10:34:31 2008 Subject: [Histonet] GFP and decalcification Message-ID: <002401c89be9$84d8b8e0$6401a8c0@DHXTS541> The decalcifier you used for two days is rather strong (8% nitric acid and 70% alcohol [made from 92%?]) The alcohol is added to the acid in order to slow down the effects of the acid on tissues but the nitric acid may have really damaged the GFP. Then you processed and embedded in paraffin? You could try EDTA, using 10 to 14% tetrasodium EDTA (this will have a beginning alkaline pH) with pH adjusted down to pH 7 to 7.4 using glacial acetic acid. THis is much slower, but also less damaging. You might be lucky and retain GFP fluorescence. You should do endpoint testing to know when bone is decalcified. There is a clever weight loss/weight gain endpoint test originally used to test nitric acid decalcification, but works nicely with EDTA. I will be happy to send that as an attachment via private email. If you have an xray unit or microCT scanner, you can check endpoint with these units. GFP is also sensitive to alcohols and heat, along with pH, so you may have damaged the GFP not only with the strong mineral acid decalcifier but also the processing reagents. The GFP is still there, but doesn't fluoresce anymore. If you try EDTA decalcification, I suggest you do an Rabbit antiGFP followed by an antiRabbit secondary labelled with Alexa 488 or FITC. This way you locate the GFP site and match its fluorescent signal with a matching color (green like the GFP) If your bone has been fixed in neutral buffered formalin, you will probably experience aldehyde induced autofluorescence which makes viewing the FITC signal a bit more difficult unless you can eliminate the autofluorescence chemically or by settings on a confocal or spectral imaging setup. Go to the IHCworld website - there is a superb discussion of autofluorescence and how to reduce this problem. Good luck Gayle M. Callis HTL/HT/MT(ASCP) Bozeman, MT From elizabeth.heimrich <@t> bms.com Fri Apr 11 10:52:27 2008 From: elizabeth.heimrich <@t> bms.com (Elizabeth M Heimrich) Date: Fri Apr 11 10:52:44 2008 Subject: [Histonet] Routes to take HT exams and qualifications Message-ID: <47FF893B.5050608@bms.com> Fellow histonetters, The IU program _*does*_ require college level bio and chemistry. I have a friend who tried to register for the course and was turned down due to lack of qualifications in the bio and chem areas. HS Chem and Bio don't cut it, at least for the IU program! If anyone is interested I have the Histotechnology Program Directors name and number for the distance learning course. IU also hasavailable an AA degree in Histotechnology at the University of Medicine campus. Beth From TJJ <@t> Stowers-Institute.org Fri Apr 11 10:54:50 2008 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Fri Apr 11 10:55:20 2008 Subject: [Histonet] Re: Antigenicity lost with decalcification Message-ID: We use Immunocal decalcifier (Decal Corporation) with our mouse tissues and are able to demonstrate GFP with antibody staining just fine. It is a proprietary formic acid mixture, and after fixation is gentle to most antigens. Hope this helps! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From anh2006 <@t> med.cornell.edu Fri Apr 11 11:12:00 2008 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Fri Apr 11 11:12:09 2008 Subject: [Histonet] Ki-67 for mouse In-Reply-To: <2264717ADC396742A0FF0AAB674F9A0D486C46@7THWAVE-SERVER.7thwave.local> References: <2264717ADC396742A0FF0AAB674F9A0D486C46@7THWAVE-SERVER.7thwave.local> Message-ID: DAKO clone Tec-3, it's gorgeous on FFPE sections. >Can someone recommend a good Ki-67 antibody for use on mouse tissue? > >Thanks. > > >This communication is intended solely for the use of the addressee >and may contain information that is legally privileged, confidential >or exempt from disclosure. If you are not the intended recipient, >please note that any dissemination, distribution, or copying of this >communication is strictly prohibited. Anyone who receives this >message in error should notify the sender immediately and delete it >from his or her computer >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- From anh2006 <@t> med.cornell.edu Fri Apr 11 11:13:31 2008 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Fri Apr 11 11:13:34 2008 Subject: [Histonet] GFP and decalcification In-Reply-To: <002401c89be9$84d8b8e0$6401a8c0@DHXTS541> References: <002401c89be9$84d8b8e0$6401a8c0@DHXTS541> Message-ID: We use 10% EDTA, pH 6.95 on PFA perfused and then submersion fixed murine bones and get nice GFP signal. >The decalcifier you used for two days is rather strong (8% nitric >acid and 70% alcohol [made from 92%?]) The alcohol is added to the >acid in order to slow down the effects of the acid on tissues but >the nitric acid may have really damaged the GFP. Then you >processed and embedded in paraffin? > >You could try EDTA, using 10 to 14% tetrasodium EDTA (this will have >a beginning alkaline pH) with pH adjusted down to pH 7 to 7.4 using >glacial acetic acid. THis is much slower, but also less damaging. >You might be lucky and retain GFP fluorescence. You should do >endpoint testing to know when bone is decalcified. There is a >clever weight loss/weight gain endpoint test originally used to test >nitric acid decalcification, but works nicely with EDTA. I will be >happy to send that as an attachment via private email. If you have >an xray unit or microCT scanner, you can check endpoint with these >units. > >GFP is also sensitive to alcohols and heat, along with pH, so you >may have damaged the GFP not only with the strong mineral acid >decalcifier but also the processing reagents. The GFP is still >there, but doesn't fluoresce anymore. > >If you try EDTA decalcification, I suggest you do an Rabbit antiGFP >followed by an antiRabbit secondary labelled with Alexa 488 or FITC. >This way you locate the GFP site and match its fluorescent signal >with a matching color (green like the GFP) > > If your bone has been fixed in neutral buffered formalin, you will >probably experience aldehyde induced autofluorescence which makes >viewing the FITC signal a bit more difficult unless you can >eliminate the autofluorescence chemically or by settings on a >confocal or spectral imaging setup. Go to the IHCworld website - >there is a superb discussion of autofluorescence and how to reduce >this problem. > >Good luck > >Gayle M. Callis >HTL/HT/MT(ASCP) >Bozeman, MT >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- From integrated.histo <@t> gmail.com Fri Apr 11 11:49:57 2008 From: integrated.histo <@t> gmail.com (Cindy DuBois) Date: Fri Apr 11 11:50:03 2008 Subject: [Histonet] RE: Salary / Temp positions Message-ID: <5d9104a30804110949m7e6e5a2cj3e526f6be97c63ec@mail.gmail.com> I have trained both people with and without a biology / chemistry background. The ones with the background are so much easier to work with. The ones without take so much more training that I sometimes wonder if it is worth it. I guess it probably is if you are short-staffed. I graduated from an Electron Microscopy Training program many years ago. There were so many similarities between light and electron microscopy I did not have a difficult time with the test and obtaining HT. I have a co-worker w/o any biology or chemistry background who took the test 3 times and was never able to pass it (prior to the new rules). I still get blank looks from this person when I try and explain why a stain didn't work, or the tissue didn't process properly. My employer is now realizing he has to pay more to get the quality of employee he wants. This is good for me because last time he hired an HT he had to give me a raise (as a supervisor) to keep my salary above the new hire. Cindy From MadaryJ <@t> MedImmune.com Fri Apr 11 12:06:27 2008 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Fri Apr 11 12:06:45 2008 Subject: [Histonet] Histology Internship in Gaithersburg Md available Message-ID: Hey netters, I have an intersnhip position available for a coolege student or recent college grad at Medimmune in Gaithersburg Md. It is hard to find decent internship programs out there, so here is a chance for a deserving youngster. I do all the hands on for routine paraffin histology through some specials and I match it with reading chapters in different books on whatever subject they are learning. The old Dual trianing like the military does, read about microtomy while you are learing to do it in the lab. I will tell you that I am reading some of this stuff. I have found some of these kids who want training and job experience are not taking it seriously at all. It is like they have a sense of entitlement, like they are doing us a favor by accepting the internship. I had a kid a while back tell me he could not make it in for his first night at work because he and his friends had been playing the same video game for 7 yrs and they did not want to break the streak! Yet another at another job while I was interviewing on the phone was laughing and joking with her friends while I was trying to do a phone interview! I wonder if someone has ASCP certifications like me and I train someone and have them do a program in the lab that allows them to learn histology and do the didactic portion does that count for anything even if the program is informal? Lets face it some interns end up learning in the lab and reading a few books and get the theory down along with the hands on so well that we would hire them if we could but we can't for budgetary reasons. I mean if a decent certified tech puts his or her stamp of approval on a tech, seems like employers would recognize that. Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Laboratory One Medimmune Way Gaithersburg, MD 20878 ph 301.398.4745/6360 fx 301.398.9745 To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From Dorothy.L.Webb <@t> HealthPartners.Com Fri Apr 11 12:22:52 2008 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Fri Apr 11 12:22:58 2008 Subject: [Histonet] Microwave processing Message-ID: <0E394B648E5284478A6CCB78E5AFDA2705635614@hpes1.HealthPartners.int> TAT is not the reason we are so into microwave processing, but rather to make our lab more of a LEAN process. By not having everything come off of the processor in the AM, we have "batches" we do throughout the day. We have 2 processors that run a routine overnight run. While those are being embedded and cut, we have a short run of derms going and several microwave runs of cell blocks, GI and other small bx.s. The timing is such that when the techs are done with the overnight run, the other runs are ready to be embedded and/or cut! The flow is so smooth and we really enjoy working with the microwave processor!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From liz <@t> premierlab.com Fri Apr 11 13:50:57 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Apr 11 13:51:05 2008 Subject: [Histonet] Factor VIII staining Message-ID: Hello everyone I have a question about factor VIII staining in general. As I understand it, F VIII will decorate endothelial cells and platelets correct? Anything else? In particular, I am curious about areas which contain fibrin....will the fibrin itself pick up the F VIII, or is it the platelets/plasma that is making it positive? I've looked in the literature, but can't find anything definitive and any help would be appreciated. Everyone have a great weekend and thanks in advance. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 From jkiernan <@t> uwo.ca Fri Apr 11 14:06:54 2008 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Apr 11 14:06:58 2008 Subject: [Histonet] granules after X-gal staining Message-ID: Beta galactosidase is present in normal cells but, like other lysosomal enzymes, it is active at low pH (4 to 5.5). The bacterial enzyme, whose gene is introduced into cells by infection or transfection, has a higher pH optimum (7.5). With the indigogenic medium at this higher pH the lysosomal enzyme should not show up unless the incubation is unduly prolonged. The bacterial enzyme is also used as a label for secondary antibodies in multi-colour imunohistochemistry. As you suggest, a "normal" tissue without the bacterial enzyme would be a sensible negative control. See Biotech. Histochem. 82(2):73-103 (2007) for a review of X-gal and other indigogenic substrates. (This whole issue of the journal is about indigo and related dyes; it makes for some interesting reading that goes beyond the field of histotechnology.) John A. Kiernan Dept of Anatomy & Cell Biology The University of Western Ontario LONDON, Canada N6A 5C1 http://publish.uwo.ca/~jkiernan/ http://instruct.uwo.ca/anatomy/530/ = = = ----- Original Message ----- From: koellingr@comcast.net Date: Friday, April 4, 2008 0:18 Subject: Re: [Histonet] granules after X-gal staining To: Yves Heremans , histonet@lists.utsouthwestern.edu > I haven't seen any replies and I am scientifically > curious. The beta-galactosidase is simply the enzyme that > splits x-gal to eventually produce that classical blue chromogen > deposit. The acinar cells of pancreas are laden with known > ..ase's (lipoxygenase, proteases, amylase, lipase, elastase, > tryptase, etc, etc ase's) and probably unidentified ones. > Is it possible that some promiscuous enzyme is substituting > enzymatically for beta-galactosidase to get your staining of > tiny round blue granules in cytoplasm. If you are working > in frozens, your enzymes could all be very active. Have > looked at lots of x-gal staining but never in pancreas. > Have you stained a normal, not b-gal expressing, mouse pancreas? > Am curious and hope someone has done this. > > Ray Koelling > PhenoPath Labs > Seattle, WA > > -------------- Original message -------------- > From: Yves Heremans > > > Dear Histonetters, > > > > Does anyone know why I am getting granules (tiny, round blue > granules > > in the cytoplasm) after X-gal staining on frozen sections of > mouse > > pancreas ? > > > > Regards, > > > > Yves > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From detmar <@t> mshri.on.ca Fri Apr 11 14:12:48 2008 From: detmar <@t> mshri.on.ca (Jacqui Detmar) Date: Fri Apr 11 14:13:16 2008 Subject: [Histonet] Ki-67 for mouse In-Reply-To: References: <2264717ADC396742A0FF0AAB674F9A0D486C46@7THWAVE-SERVER.7thwave.local> Message-ID: Hi there. Re: this clone on mouse FFPE tissues, I find that I need to do citrate buffer retrieval in a microwave pressure cooker in order to see binding. Do you find this to be true for you, too? If not, what method of antigen retrieval do you use? I find the pressure cooker method makes my sections look crappy...poor nuclear counterstaining.... Jacqui Jacqui Detmar, Post-doctoral Fellow Samuel Lunenfeld Research Institute, room 876 Mount Sinai Hospital 600 University Avenue Toronto, ON, Canada M5G 1X5 phone: 416-586-4800 x2451/x2290 fax: 416-586-8588 email: detmar@mshri.on.ca -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea Hooper Sent: Friday, April 11, 2008 12:12 PM To: Michele Wich; Histonet Subject: Re: [Histonet] Ki-67 for mouse DAKO clone Tec-3, it's gorgeous on FFPE sections. >Can someone recommend a good Ki-67 antibody for use on mouse tissue? > >Thanks. > > >This communication is intended solely for the use of the addressee >and may contain information that is legally privileged, confidential >or exempt from disclosure. If you are not the intended recipient, >please note that any dissemination, distribution, or copying of this >communication is strictly prohibited. Anyone who receives this >message in error should notify the sender immediately and delete it >from his or her computer >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anh2006 <@t> med.cornell.edu Fri Apr 11 14:50:08 2008 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Fri Apr 11 14:50:18 2008 Subject: [Histonet] Ki-67 for mouse In-Reply-To: References: <2264717ADC396742A0FF0AAB674F9A0D486C46@7THWAVE-SERVER.7thwave.local> Message-ID: I am a loyal advocate of the veggie steamer with DAKOs TRS solution - have been for years. It really revolutionized the quality of my stainings. 20 minutes at 95 deg C with a 20 minute cool down on the bench. Gorgeous results, I can send you a pic under separate cover if you like. At 3:12 PM -0400 4/11/08, Jacqui Detmar wrote: >Hi there. Re: this clone on mouse FFPE tissues, I find that I need to >do citrate buffer retrieval in a microwave pressure cooker in order to >see binding. Do you find this to be true for you, too? If not, what >method of antigen retrieval do you use? I find the pressure cooker >method makes my sections look crappy...poor nuclear counterstaining.... > > >Jacqui > >Jacqui Detmar, Post-doctoral Fellow >Samuel Lunenfeld Research Institute, room 876 >Mount Sinai Hospital >600 University Avenue >Toronto, ON, Canada >M5G 1X5 > >phone: 416-586-4800 x2451/x2290 >fax: 416-586-8588 >email: detmar@mshri.on.ca -- From dcojita <@t> tampabay.rr.com Fri Apr 11 18:52:42 2008 From: dcojita <@t> tampabay.rr.com (dcojita@tampabay.rr.com) Date: Fri Apr 11 18:59:06 2008 Subject: [Histonet] temporary, part time, full time position in Tampa In-Reply-To: Message-ID: If anyone is interested in temporary, part-time, or permanent position in Tampa, Florida, please let me know. Diane 813-490-7215 From dennisapplegate <@t> gmail.com Fri Apr 11 19:03:15 2008 From: dennisapplegate <@t> gmail.com (dennis applegate) Date: Fri Apr 11 19:03:19 2008 Subject: [Histonet] Help with a brown and brenn, Brown and Hopps question Message-ID: Hello everyone, I am a student of histology at clover park technical college and we have a task to complete. We have to find out who the Brown of the Brown and Brenn, Brown and Hopps stain is. If anyone can point me into the right direction I would greatly appreciate it. I have found no info on the internet thus far. Thanks DA From gayle.callis <@t> bresnan.net Fri Apr 11 19:49:45 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Fri Apr 11 19:49:43 2008 Subject: [Histonet] Help with a brown and brenn, Brown and Hopps question References: Message-ID: <001401c89c37$1a57b8b0$6501a8c0@DHXTS541> Brown RC and Hopps HC, Staining of bacteria in tissue sections: a reliable Gram stain method. Am. J. Clin Path. 60:234-240, 1973. Brown RC and Hopps HC both worked for the Geographic Divistion, AFIP, Washington DC, as stated by Lee Luna editor of AFIP Manual. Luna indicated te method would be published later, as it was in 1973 "Brown" for the Brown and Brenn original reference was Brown JH and Brenn L. Bull. Johns Hopkins Hospital, 48:69-73, 1931 (AFIP modification) As for the Brown and Brenn modification, the whole reference is Taylor RD. Modification of the Brown and Brenn Gram stain for the differential saining of gram positive and gram negative bacteria in tissue sections. AM. J. Clin Patholo 46:472-476, 1966. This was found in Sheehan and Hrapchak Theory and Practice of Histotechnology reference list for chapter on Microorganisms . Have you tried Google Scholar for searching? Gayle Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "dennis applegate" To: Sent: Friday, April 11, 2008 6:03 PM Subject: [Histonet] Help with a brown and brenn, Brown and Hopps question > Hello everyone, > I am a student of histology at clover park technical college and we have a > task to complete. We have to find out who the Brown of the Brown and > Brenn, > Brown and Hopps stain is. > If anyone can point me into the right direction I would greatly appreciate > it. I have found no info on the internet thus far. > > Thanks > > DA > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Fri Apr 11 20:00:27 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Fri Apr 11 20:00:38 2008 Subject: [Histonet] Ki-67 for mouse Message-ID: <582736990804111800kb3dc568p1cf166e75f1309f2@mail.gmail.com> Hi, I highly reccommend the rabbit monoclonal KI-67 from Thermo. It has been great! It works in mouse, human and a few other critters. It looks great too. Unfortunately I don't have the catalog # as I'm away from the lab, but I'm sure they would be able to help at customer service if you told them that you want the rabbit monoclonal that works in mouse tissue. good luck, Amos Brooks Message: 11 Date: Fri, 11 Apr 2008 10:20:45 -0500 From: "Michele Wich" Subject: [Histonet] Ki-67 for mouse To: Message-ID: <2264717ADC396742A0FF0AAB674F9A0D486C46@7THWAVE-SERVER.7thwave.local> Content-Type: text/plain; charset="us-ascii" Can someone recommend a good Ki-67 antibody for use on mouse tissue? Thanks. From dennisapplegate <@t> gmail.com Fri Apr 11 20:03:07 2008 From: dennisapplegate <@t> gmail.com (dennis applegate) Date: Fri Apr 11 20:03:10 2008 Subject: [Histonet] Thanks for the replys Message-ID: Thanks for the replies, I will look in that page for the information. I really appreciate it :) DA From JMacDonald <@t> mtsac.edu Fri Apr 11 23:47:54 2008 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Apr 11 23:48:00 2008 Subject: [Histonet] Salary / Temp positions In-Reply-To: Message-ID: Not all college is as expensive as people might think. We have an AS degree in Histotechnology. We are a NAACLS accredited program. The students are required to take about 70 units of classes to complete their degree. This will vary with the math and English competencies that they come in with. The cost is $20 per unit, plus books and fees. There are also many scholarships available at our institution that will cover many of the students expenses. Our graduates are starting with very decent salaries so the cost of college has not set them back financially, but provided them with many career opportunities. Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu "Cory Collins" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/10/2008 12:57 PM To cc Subject [Histonet] Salary / Temp positions Hey Cindy, I think you have a valid point with the cost of 2-4 years worth of schooling. It is very expensive. But we're not the only ones faced with the problem of low pay out of college and student loans. Teachers get paid far less than what they deserve and they still do it and there is a huge shortage of teachers, just like techs. This is where we are at in the profession and it's not going to change, this is the answer that the powers that be have come up with. I think it's important for a tech to have a strong background in science. This will certainly help them to be able to troubleshoot problems in the lab. The histology world is getting much more complex with the use of IHC, ISH, FISH, image analysis and whatever else is on the horizon. I'm not saying that a tech that doesn't have formal training can't learn these things on the job, I've taught a few techs these areas that didn't know squat about science before coming into the lab and they've done great. But to improve our pay over the next couple of decades, I think ASCP is right on with the requirements. Unfortunately that means a shortage of techs and it'll probably be that way for the next several years. Our answer to ASCP's requirements is getting the word out to anyone that will listen about histology as a career, especially young people. I graduated almost 9 years ago and am still paying on my student loans, I have a ways to go. The good news is the lenders give you plenty of time to do it and you can get on a payment program where the payments start low and increase over time. This allows you to make a living right out of school and then pay more when you should be making more, a few years after graduation. Just my two cents...I think a college degree is well worth the price of admission. The experience along with the long-term earning potential makes it a good investment. Cory Collins, HT (ASCP) QIHC Histology Lab Supervisor Digestive Health Associates of Texas 7920 Elmbrook Dr, Suite 104 Dallas, TX 75247 P: (214)689-5960 x 311 F: (214)689-3804 www.DHAT.com Date: Thu, 10 Apr 2008 10:12:54 -0700 From: "Cindy DuBois" Subject: [Histonet] Salary / Temp positions To: histonet@lists.utsouthwestern.edu Message-ID: <5d9104a30804101012y48035d2cwefc3148107838ed9@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 If we think about the cost of 2-4 years of college to obtain a degree in order to qualify for the Histology test, most of us would be in debt when finished. Then look at our salary and you can see how the requirements just aren't supported by the salary. Most of the students will come out of college owing on student loans. The salaries they would receive as new histotech would allow them to pay off their student loanswhile maintaining a decent living (at least here in CA). With both my sons in college (using student loans) we had to take a serious look at the final amount they will owe when the graduate and compare it to what they would be earning. I am not sure what the solution is. Any ideas? Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kim.Osullivan <@t> med.monash.edu.au Sat Apr 12 02:18:10 2008 From: Kim.Osullivan <@t> med.monash.edu.au (Kim O'Sullivan) Date: Sat Apr 12 02:18:18 2008 Subject: [Histonet] Antigen retrieval Message-ID: <58.175.44.134.1207984531@my.monash.edu.au> Hi, Is anyone familiar with doing antigen retrieval overnight at 80 degrees celsius in 0.1M Tris/HCL pH 9.0? My question is: What is the best way to do this in terms of safety etc. A waterbath? Hot oven? Any idea's appreciated. Kim O'Sullivan Monash University Melbourne Australia From Pathrm35 <@t> comcast.net Sat Apr 12 07:43:50 2008 From: Pathrm35 <@t> comcast.net (Pathrm35@comcast.net) Date: Sat Apr 12 07:44:00 2008 Subject: [Histonet] Salary / Temp positions Message-ID: <041220081243.25191.4800AE860001795E000062672215553894CACC039D089B0EAF@comcast.net> My company offers $2,500.00 per year for tuition assistance for job related courses/degrees. This is helpful esp. for the students in the histology program. Ron Martin -------------- Original message -------------- From: Jennifer MacDonald > Not all college is as expensive as people might think. We have an AS > degree in Histotechnology. We are a NAACLS accredited program. The > students are required to take about 70 units of classes to complete their > degree. This will vary with the math and English competencies that they > come in with. The cost is $20 per unit, plus books and fees. There are > also many scholarships available at our institution that will cover many > of the students expenses. Our graduates are starting with very decent > salaries so the cost of college has not set them back financially, but > provided them with many career opportunities. > > Jennifer MacDonald > Director, Histotechnician Training Program > Mt. San Antonio College > 1100 N. Grand Ave. > Walnut, CA 91789 > (909) 594-5611 ext. 4884 > jmacdonald@mtsac.edu > > > > > "Cory Collins" > Sent by: histonet-bounces@lists.utsouthwestern.edu > 04/10/2008 12:57 PM > > To > > cc > > Subject > [Histonet] Salary / Temp positions > > > > > > > Hey Cindy, > > > > I think you have a valid point with the cost of 2-4 years worth of > schooling. It is very expensive. But we're not the only ones faced > with the problem of low pay out of college and student loans. Teachers > get paid far less than what they deserve and they still do it and there > is a huge shortage of teachers, just like techs. This is where we are > at in the profession and it's not going to change, this is the answer > that the powers that be have come up with. > > > > I think it's important for a tech to have a strong background in > science. This will certainly help them to be able to troubleshoot > problems in the lab. The histology world is getting much more complex > with the use of IHC, ISH, FISH, image analysis and whatever else is on > the horizon. I'm not saying that a tech that doesn't have formal > training can't learn these things on the job, I've taught a few techs > these areas that didn't know squat about science before coming into the > lab and they've done great. But to improve our pay over the next couple > of decades, I think ASCP is right on with the requirements. > Unfortunately that means a shortage of techs and it'll probably be that > way for the next several years. Our answer to ASCP's requirements is > getting the word out to anyone that will listen about histology as a > career, especially young people. > > > > I graduated almost 9 years ago and am still paying on my student loans, > I have a ways to go. The good news is the lenders give you plenty of > time to do it and you can get on a payment program where the payments > start low and increase over time. This allows you to make a living > right out of school and then pay more when you should be making more, a > few years after graduation. > > > > Just my two cents...I think a college degree is well worth the price of > admission. The experience along with the long-term earning potential > makes it a good investment. > > > > > > Cory Collins, HT (ASCP) QIHC > > Histology Lab Supervisor > > Digestive Health Associates of Texas > > 7920 Elmbrook Dr, Suite 104 > > Dallas, TX 75247 > > P: (214)689-5960 x 311 > > F: (214)689-3804 > > www.DHAT.com > > > > > > > > Date: Thu, 10 Apr 2008 10:12:54 -0700 > > From: "Cindy DuBois" > > Subject: [Histonet] Salary / Temp positions > > To: histonet@lists.utsouthwestern.edu > > Message-ID: > > <5d9104a30804101012y48035d2cwefc3148107838ed9@mail.gmail.com> > > Content-Type: text/plain; charset=ISO-8859-1 > > > > If we think about the cost of 2-4 years of college to obtain a degree in > > order to qualify for the Histology test, most of us would be in debt > when > > finished. Then look at our salary and you can see how the requirements > just > > aren't supported by the salary. Most of the students will come out of > > college owing on student loans. The salaries they would receive as new > > histotech would allow them to pay off their student loanswhile > maintaining a > > decent living (at least here in CA). > > With both my sons in college (using student loans) we had to take a > serious > > look at the final amount they will owe when the graduate and compare it > to > > what they would be earning. > > I am not sure what the solution is. Any ideas? > > > > Cindy > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sat Apr 12 10:03:27 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Apr 12 10:03:31 2008 Subject: [Histonet] Antigen retrieval In-Reply-To: <58.175.44.134.1207984531@my.monash.edu.au> Message-ID: <729018.2869.qm@web65711.mail.ac4.yahoo.com> Never heard of such a procedure. On the other hand, unless you are dealing with a HUGE amount of buffer, it will evaporate during such a prolonged period of time. Check your reference, I think something is not right. Ren? J. Kim O'Sullivan wrote: __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From gayle.callis <@t> bresnan.net Sat Apr 12 10:25:35 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Sat Apr 12 10:25:32 2008 Subject: [Histonet] Antigen retrieval References: <729018.2869.qm@web65711.mail.ac4.yahoo.com> Message-ID: <001a01c89cb1$7433b7b0$6501a8c0@DHXTS541> Rene and Kim, If you cover the waterbath (they come with covers) and also the slide container (coplin jars, whatever), it should eliminate evaporation. Waterbath methods are also found in the literature. Jules Elias has published a method but I do not recall the temperature used overnight. I have several waterbath heated methods that use higher temperatures for shorter times if Kim is interested. I will be happy to provide those via private message with attachment. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Rene J Buesa" To: "Kim O'Sullivan" ; Sent: Saturday, April 12, 2008 9:03 AM Subject: Re: [Histonet] Antigen retrieval > Never heard of such a procedure. On the other hand, unless you are dealing > with a HUGE amount of buffer, it will evaporate during such a prolonged > period of time. > Check your reference, I think something is not right. > Ren? J. > > Kim O'Sullivan wrote: > > > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bakevictoria <@t> gmail.com Sat Apr 12 11:10:15 2008 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Sat Apr 12 11:10:22 2008 Subject: [Histonet] Antigen retrieval In-Reply-To: <729018.2869.qm@web65711.mail.ac4.yahoo.com> References: <58.175.44.134.1207984531@my.monash.edu.au> <729018.2869.qm@web65711.mail.ac4.yahoo.com> Message-ID: <4f016b690804120910s5a7061dm19de8812812d69fd@mail.gmail.com> Rene and Kim It's not a routine procedure, but what Kim hasn't said is why this is needed. Yes in certain instances - histochemically speaking - some incubation's need to be done overnight but not at quite that high a temp. Immunohistochemically - I've done it but it took time to work out the best method to achieve When we've had to do these it was done in a waterbath for no more than 12 hours and the bath covered loosely either by a tent cover (it comes with the waterbath) or aluminum foil to hold the humidity in. The amount of buffer required for the slides will be pretty hefty, but it's more important to make sure you have a waterbath that can actually maintain the temperature and surrounding moisture so that the buffer won't evaporate. This is going to require some testing during the day to see how best to make this work. Kim if you are looking to do a large volume of slides make sure you use a pyrex dish that fits into the waterbath or one that can be supported in a bath - sort of like using a double boiler when you're melting chocolate (sorry for the less than scientific description)- so that you don't burn out the heating element. I also put in blank slides to best mimic the volume of work I'd be doing. The bath was set up first thing in the morning and both the temp and water levels would be monitored during the day this way we could best gauge and write up our protocol. I know that there is literature on doing this procedure by Dr. Elias, but I don't have it in front of me. I hope this helps, if there is anything else I can do please let me know. Vikki Baker On 4/12/08, Rene J Buesa wrote: > Never heard of such a procedure. On the other hand, unless you are dealing with a HUGE amount of buffer, it will evaporate during such a prolonged period of time. > Check your reference, I think something is not right. > Ren? J. > > Kim O'Sullivan wrote: > > > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From spanish_fly1981 <@t> yahoo.com Sat Apr 12 16:48:21 2008 From: spanish_fly1981 <@t> yahoo.com (Tate Maria) Date: Sat Apr 12 16:48:25 2008 Subject: [Histonet] Demand for tech Message-ID: <566000.8768.qm@web58701.mail.re1.yahoo.com> Hello!! Is there no need for Histotech in the Alabama, Georgia, Florida panhandle area??? especially Alabama! Do you need to be registered to work there? It seems like those areas are really dry for job opening! Thanks, Maria __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From jvirgil5 <@t> msn.com Sat Apr 12 17:27:45 2008 From: jvirgil5 <@t> msn.com (virgil hernandez) Date: Sat Apr 12 17:27:54 2008 Subject: [Histonet] please remove me Message-ID: please remove me from this listserv Thank you From Kim.Osullivan <@t> med.monash.edu.au Sat Apr 12 19:54:55 2008 From: Kim.Osullivan <@t> med.monash.edu.au (Kim O'Sullivan) Date: Sat Apr 12 19:55:05 2008 Subject: [Histonet] Antigen retrieval References: <001a01c89cb1$7433b7b0$6501a8c0@DHXTS541> Message-ID: <58.175.44.134.1208047484@my.monash.edu.au> Hi Gayle, Renee and others, Thanks for the reply's. I am staining for KIM-1/TIM-1 and all the literature states that it has to be subjected to antigen retrieval at 80 degree's celsius overnight,- I was just worried that I would come back to the lab the next day to find either the lab had burned down or my tissue ruined! Kim Gayle Callis wrote:> > Rene and Kim, > > If you cover the waterbath (they come with covers) and also the slide > container (coplin jars, whatever), it should eliminate evaporation. > > Waterbath methods are also found in the literature. Jules Elias has > published a method but I do not recall the temperature used overnight. > > I have several waterbath heated methods that use higher temperatures for > shorter times if Kim is interested. I will be happy to provide those via > private message with attachment. > > Gayle M. Callis > HTL/HT/MT(ASCP) > Bozeman MT 59715 > > > ----- Original Message ----- > From: "Rene J Buesa" > To: "Kim O'Sullivan" ; > > Sent: Saturday, April 12, 2008 9:03 AM > Subject: Re: [Histonet] Antigen retrieval > > >> Never heard of such a procedure. On the other hand, unless you are >> dealing >> with a HUGE amount of buffer, it will evaporate during such a >> prolonged >> period of time. >> Check your reference, I think something is not right. >> René J. >> >> Kim O'Sullivan wrote: >> >> >> >> __________________________________________________ >> Do You Yahoo!? >> Tired of spam? Yahoo! Mail has the best spam protection around >> http://mail.yahoo.com >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bakevictoria <@t> gmail.com Sat Apr 12 20:11:16 2008 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Sat Apr 12 20:11:19 2008 Subject: [Histonet] Antigen retrieval In-Reply-To: <58.175.44.134.1208047484@my.monash.edu.au> References: <001a01c89cb1$7433b7b0$6501a8c0@DHXTS541> <58.175.44.134.1208047484@my.monash.edu.au> Message-ID: <4f016b690804121811l3c92bbeeh190d4a9d1d92e1b3@mail.gmail.com> Ahhhhh! Now then I can relate to that! I had a lab partner who left a bunsen burner on one evening and the cleaning staff called me at home to find out why it was on and then how to turn it off!!! Fear not - this is small stuff. Best results wished for you. Vikki On 4/12/08, Kim O'Sullivan wrote: > Hi Gayle, Renee and others, > > Thanks for the reply's. I am staining for KIM-1/TIM-1 and all the literature states that it has to be subjected to antigen retrieval at 80 degree's celsius overnight,- I was just worried that I would come back to the lab the next day to find either the lab had burned down or my tissue ruined! > > Kim > > Gayle Callis wrote:> > > Rene and Kim, > > > > If you cover the waterbath (they come with covers) and also the slide > > container (coplin jars, whatever), it should eliminate evaporation. > > > > Waterbath methods are also found in the literature. Jules Elias has > > published a method but I do not recall the temperature used overnight. > > > > I have several waterbath heated methods that use higher temperatures for > > shorter times if Kim is interested. I will be happy to provide those via > > private message with attachment. > > > > Gayle M. Callis > > HTL/HT/MT(ASCP) > > Bozeman MT 59715 > > > > > > ----- Original Message ----- > > From: "Rene J Buesa" > > To: "Kim O'Sullivan" ; > > > > Sent: Saturday, April 12, 2008 9:03 AM > > Subject: Re: [Histonet] Antigen retrieval > > > > > >> Never heard of such a procedure. On the other hand, unless you are > >> dealing > >> with a HUGE amount of buffer, it will evaporate during such a > >> prolonged > >> period of time. > >> Check your reference, I think something is not right. > >> Ren? J. > >> > >> Kim O'Sullivan wrote: > >> > >> > >> > >> __________________________________________________ > >> Do You Yahoo!? > >> Tired of spam? Yahoo! Mail has the best spam protection around > >> http://mail.yahoo.com > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gu.lang <@t> gmx.at Sun Apr 13 11:16:57 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sun Apr 13 11:17:09 2008 Subject: [Histonet] alcianblue - skinpicture 2nd try Message-ID: <000301c89d81$ce5be4d0$eeeea8c0@dielangs.at> Hi dear listmembers, We struggle with the "right" image of a good alcianblue stain of skin with lupus. We use a commercial 1% alcianblue, pH 2,5 (10 min incubation, wash in aqua dest., 5 min nuclear fast red, wash, dehydrate, clear, coverslip). We switched just two months ago from the automated Nexes stainer to the (good old) manual method. Now my pathologists complain about "too much blue" in the corium. I say, there must be a staining of the hyaluronic acid and other acid mucosubstances surrounding the collagen fibers. They say, they only want to see the mucin stained, that is typical for the lupus, in intense blue and the surrounding should be colourless. So, can somebody provide me with a picture of a good alcianblue stain of skin with lupus? (or just normal skin)- please. Or give me any helpful hint. Thanks in advance Gudrun Lang From jwray78 <@t> gmail.com Sun Apr 13 13:34:54 2008 From: jwray78 <@t> gmail.com (Josh Wray) Date: Sun Apr 13 13:34:58 2008 Subject: [Histonet] PAS/D digestion problem Message-ID: <883927cf0804131134w20a5df60xe82bd468a8011774@mail.gmail.com> Hello, Does anyone out there have a problem with their digesting of a PAS/D? I was wondering if I could get some feedback on some peoples methods of digestion that works well for them to pass along to our Histo Dept. Thanks, Josh Wray HT(ASCP) Esoterics Laboratory From rjbuesa <@t> yahoo.com Sun Apr 13 13:50:07 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Apr 13 13:50:13 2008 Subject: [Histonet] PAS/D digestion problem In-Reply-To: <883927cf0804131134w20a5df60xe82bd468a8011774@mail.gmail.com> Message-ID: <390231.61461.qm@web65708.mail.ac4.yahoo.com> John: I always used successfully the following procedure: a- prepare a "diastase buffer": anhydrous sodium chloride (8 g) + anhydrous dibasic sodium phosphate (0.282 g) + anhydrous monobasic sodium phosphate (1.974 g) + distilled water UP TO 1 liter. This buffer will have a pH @ 6 and has to be kept in the refrigerator. b- glycogen digesting solution: take 40 mL of the buffer and heat it to 37?C and add 0.5 g of diastase of malt (Fisher brand "maltin" cat.No. D22-100). c- incubate the hydrated section at 37?C for 30 minutes Proceed with the PAS, counter stain, etc., etc. Ren? J. Josh Wray wrote: __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From mohs76009 <@t> yahoo.com Sun Apr 13 16:23:38 2008 From: mohs76009 <@t> yahoo.com (Matt Bancroft) Date: Sun Apr 13 16:23:50 2008 Subject: [Histonet] PAS/D digestion problem In-Reply-To: <883927cf0804131134w20a5df60xe82bd468a8011774@mail.gmail.com> Message-ID: <249568.28643.qm@web63403.mail.re1.yahoo.com> I know that it is gross, but try using your spit Josh Wray wrote: Hello, Does anyone out there have a problem with their digesting of a PAS/D? I was wondering if I could get some feedback on some peoples methods of digestion that works well for them to pass along to our Histo Dept. Thanks, Josh Wray HT(ASCP) Esoterics Laboratory _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From marktarango <@t> gmail.com Sun Apr 13 16:54:25 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Sun Apr 13 16:54:33 2008 Subject: [Histonet] Histology vacancies In-Reply-To: <842113.49682.qm@web31305.mail.mud.yahoo.com> References: <842113.49682.qm@web31305.mail.mud.yahoo.com> Message-ID: <5b6eb13e0804131454j58f89b2fh2ab52c77a23650bb@mail.gmail.com> Do you have any On Thu, Apr 10, 2008 at 12:35 PM, Akemi Allison-Tacha akemiat3377@yahoo.comwrote: > Hello Dan in Danville, > > Having worked and lived in the SF Bay Area for a > number of years, and in Danville too, I know exactly > what you are talking about! I agree with everything > you and Patti stated. > > You may or may not be aware of it, but Governor Arnold > Schwarzenegger has mandated that ALL laboratory staff > working in health care, be certified by 2009, or was > it 2010. This includes histologists. I know of a > number of histologists that are not certified in CA, > and this is going to cause a huge problem, as well as > further shortages in histology laboratories. NY has > been dealing with this issue too. > > Akemi Allison-Tacha, BS, HT(ASCP)HTL > Client Services Manager > PhenoPath laboratories > 551 North 34th Street, Suite 100 > Seattle, WA 98103-8675 > Work: (206) 374-9000 > E-Mail: akemiat3377@yahoo.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From marktarango <@t> gmail.com Sun Apr 13 16:56:59 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Sun Apr 13 16:57:11 2008 Subject: [Histonet] Histology vacancies In-Reply-To: References: <842113.49682.qm@web31305.mail.mud.yahoo.com> Message-ID: <5b6eb13e0804131456l1ea784caq2fbde398f6001abf@mail.gmail.com> Do you have any more information on this for California? I tried to finding some info on this and couldn't come up with anything. How did you hear about this? If anything is going to do something about it, we'll have to get the word out. On Fri, Apr 11, 2008 at 8:14 AM, Luis Chiriboga wrote: > Hi all > I'm not sure how the law is set-up in CA but it is a complete mess here in > New York.......Our law, just passed in 2006 requires all "clinical > laboratory" personnel to be licensed. Briefly, the law created a > generalist > license, allowing anyone who meets the educational and test criteria to > practice in a clinical laboratory. However, there is no distinction > between > specialties, and no mention of histology. In fact the educational > curricula > proposed only requires 1 semester course in histology (microscopic) in > order > to be licensed. IN addition, anyone wishing to come to New York to > practice > in histology now has to meet the educational requirements (specific course > work) and take the generalist test (covers all areas but is mostly > CP.....from chemistry to blood banking) regardless of years in practice, > certifications or degrees. > I believe that there are a few other, if not several, states considering > licensing legislation. I highly recommend that histology people get > involved..... get your state society to represent you in this process. > Otherwise, you may end up far worse off than you already may be....... > > Luis Chiriboga PhD., HT (ASCP) QIHC > New York University School of Medicine > NYU Cancer Institute IHC Core Facility > Bellevue Hospital Center > Department of Pathology 4W27 > 27th Street & First Avenue > New York, N.Y. 10016 > V (212)562-4667 > F (212)263-2041 > Luis.Chiriboga@med.nyu.edu > > Vice President > New York State Histotechnological Society > http://www.nyhisto.org/ > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Akemi > Allison-Tacha > Sent: Thursday, April 10, 2008 2:35 PM > To: Histonet > Subject: [Histonet] Histology vacancies > > > Hello Dan in Danville, > > Having worked and lived in the SF Bay Area for a > number of years, and in Danville too, I know exactly > what you are talking about! I agree with everything > you and Patti stated. > > You may or may not be aware of it, but Governor Arnold > Schwarzenegger has mandated that ALL laboratory staff > working in health care, be certified by 2009, or was > it 2010. This includes histologists. I know of a > number of histologists that are not certified in CA, > and this is going to cause a huge problem, as well as > further shortages in histology laboratories. NY has > been dealing with this issue too. > > Akemi Allison-Tacha, BS, HT(ASCP)HTL > Client Services Manager > PhenoPath laboratories > 551 North 34th Street, Suite 100 > Seattle, WA 98103-8675 > Work: (206) 374-9000 > E-Mail: akemiat3377@yahoo.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From AnthonyH <@t> chw.edu.au Sun Apr 13 18:18:50 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Apr 13 18:20:03 2008 Subject: [Histonet] PAS/D digestion problem In-Reply-To: <883927cf0804131134w20a5df60xe82bd468a8011774@mail.gmail.com> Message-ID: The following is easy to prepare, easy to use and works every time (shelf life at least 4 months (we run out of it by then): The enzyme solution is applied to one of two sections of the tissue (preferably consecutive sections) and then both are stained by the PAS method. The presence and relative amount of glycogen in the sections can be determined by examining the extent of loss of staining in the enzyme treated section as compared with the untreated section. This amylase reagent has a long shelf life and uses an incubation time of 10 minutes at room temperature. It is suitable for formalin and Brazil's fixed paraffin sections as well as air-dried and ethanol fixed frozen sections. Controls: Liver containing glycogen Reagents: 1. Amylase Reagent Warning: Harmful, contains azide - see MSDS Alpha Amylase from Bacillus Subtilis (Fluka Cat No 10070,) 1g Oxoid PBS Tablets (Cat No BR14a) 1 tablet Distilled water 100ml Sodium Azide 0.1g This solution, once prepared is stored at 4oC when not in use. Procedure: 1. Dewax and hydrate paraffin sections, hydrate frozen sections. 2. For amylase digestion, place slides on a rack, cover sections with amylase solution and allow to incubate for 10 minutes at room temperature. 3. Wash slides well in water. 4. continue with PAS reaction. Results: * Glycogen is extracted and so loss of PAS positive staining will occur in the enzyme treated section. * Mucopolysaccharides are not extracted and so staining will be the same in both sections. Reference: V-M. Mangan, V. Farago, M. Kelly, and A. F. Henwood (2002) " An Amylase Reagent with a Long Shelf Life for the Removal of Glycogen from Tissue Sections" J Histotechnol. 25(3): 153-4. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Josh Wray Sent: Monday, 14 April 2008 4:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PAS/D digestion problem Hello, Does anyone out there have a problem with their digesting of a PAS/D? I was wondering if I could get some feedback on some peoples methods of digestion that works well for them to pass along to our Histo Dept. Thanks, Josh Wray HT(ASCP) Esoterics Laboratory _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From c.m.vanderloos <@t> amc.uva.nl Mon Apr 14 01:51:38 2008 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Mon Apr 14 01:51:54 2008 Subject: [Histonet] RE: Antigen retrieval Message-ID: Kim,How about using a Thermo PTModule for this? You can even program this thing for overnight applications! In the past we used a stove for overnight at 70C. First, a tank with HIER buffer was warmed up in the microwave and then placed in a stove (70C was the maximum setting for it) with a glass plate covering the tank. The effect of overnight antigen retrieval at 70C for the few antigens we tested was a bit different, but mostly comparable with the effect of HIER for 20 min at 98C. Some of the antigens responded very well and showed far more intense staining than after short HIER (20 min, 98C), and others responded very poor. The big advantage of overnight HIER at 70C was that sections from fatty tissue better stayed on the glass slides.Hope this info helps a bit!Cheers, ChrisChris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Sat, 12 Apr 2008 17:18:10 +1000 From: Kim O'Sullivan Subject: [Histonet] Antigen retrieval To: histonet@lists.utsouthwestern.edu Hi, Is anyone familiar with doing antigen retrieval overnight at 80 degrees celsius in 0.1M Tris/HCL pH 9.0? My question is: What is the best way to do this in terms of safety etc. A waterbath? Hot oven? Any idea's appreciated. Kim O'Sullivan Monash University Melbourne Australia From marktarango <@t> gmail.com Mon Apr 14 01:59:28 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Mon Apr 14 01:59:31 2008 Subject: [Histonet] RE: Antigen retrieval In-Reply-To: References: Message-ID: <5b6eb13e0804132359h14e403d5mc105700b54d2f3ef@mail.gmail.com> Keep in mind that the PT module can't get to above 100 C without boiling over. For some antigens you need to get it hotter than 100 C. On Sun, Apr 13, 2008 at 11:51 PM, C.M. van der Loos < c.m.vanderloos@amc.uva.nl> wrote: > Kim,How about using a Thermo PTModule for this? You can even program this > thing for overnight applications! In the past we used a stove for overnight > at 70C. First, a tank with HIER buffer was warmed up in the microwave and > then placed in a stove (70C was the maximum setting for it) with a glass > plate covering the tank. The effect of overnight antigen retrieval at 70C > for the few antigens we tested was a bit different, but mostly comparable > with the effect of HIER for 20 min at 98C. Some of the antigens responded > very well and showed far more intense staining than after short HIER (20 > min, 98C), and others responded very poor. The big advantage of overnight > HIER at 70C was that sections from fatty tissue better stayed on the glass > slides.Hope this info helps a bit!Cheers, ChrisChris van der Loos, PhD > Dept. of Pathology > Academic Medical Center M2-230 > Meibergdreef 9 > NL-1105 AZ Amsterdam > The Netherlands Date: Sat, 12 Apr 2008 17:18:10 +1000 > From: Kim O'Sullivan > Subject: [Histonet] Antigen retrieval > To: histonet@lists.utsouthwestern.edu > > Hi, > > Is anyone familiar with doing antigen retrieval overnight at 80 degrees > celsius in 0.1M Tris/HCL pH 9.0? My question is: What is the best way to do > this in terms of safety etc. A waterbath? Hot oven? Any idea's appreciated. > > Kim O'Sullivan > Monash University > Melbourne > Australia > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mmarti <@t> cmrb.eu Mon Apr 14 02:27:17 2008 From: mmarti <@t> cmrb.eu (=?iso-8859-1?Q?Marti_Gaudes=2C_Merc=E8?=) Date: Mon Apr 14 02:27:51 2008 Subject: [Histonet] Antigenecity lost with decalcification Message-ID: Thank you very much for all the advices! Merc? Mart? Gaudes Cap de Servei Histology and Bioimaging Centre de Medicina Regenerativa de Barcelona (CMR[B]) Dr. Aiguader, 88 7ena planta 08003 Barcelona mmarti@cmrb.eu Tel: +34 93 316 03 51 Fax: +34 93 224 10 83 From psanquin <@t> lugo.usc.es Mon Apr 14 06:42:16 2008 From: psanquin <@t> lugo.usc.es (Pablo Sanchez-Quinteiro) Date: Mon Apr 14 06:42:23 2008 Subject: [Histonet] Hemathoxylin free-floating Message-ID: <20080414114216.AAF6218F94A@rojo2.usc.es> Dear Histonetters, I have free floating either 30 or 90 microns thick cryostat sections, which I have immunolabelled and studied at the confocal microsope. Now I would like to counterstain them with Hematoxylin & Eosin always free-floating. I would be very grateful if you could give me a procedure. Cheers Pablo Sanchez-Quinteiro Facuty of Veterinary Sciences 27002 Lugo SPAIN From tora.bardal <@t> bio.ntnu.no Mon Apr 14 07:47:56 2008 From: tora.bardal <@t> bio.ntnu.no (Tora Bardal) Date: Mon Apr 14 07:48:17 2008 Subject: [Histonet] custom made DIG probes Message-ID: <4803527C.1090507@bio.ntnu.no> Hello I would appreciate a recommendation of company/lab who offers custom-made ribo-probes with DIG (if we send the EST sequences)? ------------------------------------------------------------------------ <>Tora Bardal NTNU Senter for fiskeri og havbruk /NTNU Center of Fisheries and Aquaculture NTNU, 7491 Trondheim Norway From anne-sophie.martinez <@t> unicaen.fr Mon Apr 14 08:34:10 2008 From: anne-sophie.martinez <@t> unicaen.fr (Anne-Sophie MARTINEZ) Date: Mon Apr 14 08:34:19 2008 Subject: [Histonet] In situ hybridization problems Message-ID: <48035D52.1000008@unicaen.fr> Dear Histonetters, I am not sure I am supposed to tackle this kind of subject here but I really need help. So, let's try! It is about in situ hybridization on slides of adult oysters. We have been doing experiments for ages and have met a lot of different problems. In particular, we struggled to have a significant signal (antisense probe) and no background (sense probe). After a couple of successfull (quite!) experiments, it stopped working, even on the same samples as before. Since then, we are not only unable to obtain a signal again, but another problem has been added: when we add the substrate of the enzyme (NBT/BCIP) the slides seem to "dribble" (as if they have been bleeched (partially) - the "blue staining" is partially in the liquid around the slide). My questions are thus: - Concerning the loosing of the signal, could it be due to the destruction of the RNA (by the Davidson's fixative which is apparently not the most appropriate, the lenght of time in paraffin (samples have been kept in paraffin since 2005), the block conservation at 4?C, the number of times the blocks have been cut ...). - Is there some way to improve the signal although the mRNA might not be highly expressed or be partially damaged? - Would somebody have had the same kind of "dribble/bleeching" problem as us? Thanks a lot for your help. I run short of ideas. Anne-Sophie From kmerriam2003 <@t> yahoo.com Mon Apr 14 08:42:33 2008 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Mon Apr 14 08:42:43 2008 Subject: [Histonet] Antigen retrieval In-Reply-To: <58.175.44.134.1208047484@my.monash.edu.au> Message-ID: <647878.42443.qm@web50305.mail.re2.yahoo.com> Hi Kim, I have done overnight retreival on a few occasions (although it was at 60-65 and not 80), wtih very good success. I have done it in an oven, with the lids from the tissue tek plastic dishes taped on or covered in foil. It probably does not matter if it is an oven or a waterbath, as long as you are confident that the temperature can be maintained properly. Another note, I have also done this with high-pH retreival (10-10.5 or so) solution, which is fine as long as the tissues are not too fatty, otherwise they can not survive such long times at that pH. Good luck, Kim Merriam Kim O'Sullivan wrote: Hi Gayle, Renee and others, Thanks for the reply's. I am staining for KIM-1/TIM-1 and all the literature states that it has to be subjected to antigen retrieval at 80 degree's celsius overnight,- I was just worried that I would come back to the lab the next day to find either the lab had burned down or my tissue ruined! Kim Gayle Callis wrote:> > Rene and Kim, > > If you cover the waterbath (they come with covers) and also the slide > container (coplin jars, whatever), it should eliminate evaporation. > > Waterbath methods are also found in the literature. Jules Elias has > published a method but I do not recall the temperature used overnight. > > I have several waterbath heated methods that use higher temperatures for > shorter times if Kim is interested. I will be happy to provide those via > private message with attachment. > > Gayle M. Callis > HTL/HT/MT(ASCP) > Bozeman MT 59715 > > > ----- Original Message ----- > From: "Rene J Buesa" > To: "Kim O'Sullivan" ; > > Sent: Saturday, April 12, 2008 9:03 AM > Subject: Re: [Histonet] Antigen retrieval > > >> Never heard of such a procedure. On the other hand, unless you are >> dealing >> with a HUGE amount of buffer, it will evaporate during such a >> prolonged >> period of time. >> Check your reference, I think something is not right. >> Ren? J. >> >> Kim O'Sullivan wrote: >> >> >> >> __________________________________________________ >> Do You Yahoo!? >> Tired of spam? Yahoo! Mail has the best spam protection around >> http://mail.yahoo.com >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Kim Merriam, MA, HT(ASCP) Cambridge, MA From anh2006 <@t> med.cornell.edu Mon Apr 14 09:07:09 2008 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Mon Apr 14 09:07:19 2008 Subject: [Histonet] Antigen retrieval In-Reply-To: <647878.42443.qm@web50305.mail.re2.yahoo.com> References: <647878.42443.qm@web50305.mail.re2.yahoo.com> Message-ID: If you are worried about your "regular" water baths evaporating (which wouldn't happen if filled satisfactorily full and covered as Gayle suggested) you can purchase "molecular" grade waterbaths just for this type of purpose. We do in situ hybridizations etc using them overnight - sometimes even several nights without any issues. When filled with enough water, they hold their temp wonderfully and with a lid there is not that much evaporation. Even so I routinely do overnight incubations for retrieval at 80 degC and haven't had any issues, but my water baths are quite deep and I fill them rather full before use. I weigh down my plastic slide container (the Tissue tek kind that holds 24 slides) with a weight and all is stable for the night. -- From b-frederick <@t> northwestern.edu Mon Apr 14 09:17:06 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon Apr 14 09:17:28 2008 Subject: [Histonet] Ki-67 for mouse In-Reply-To: <2264717ADC396742A0FF0AAB674F9A0D486C46@7THWAVE-SERVER.7thwave.local> Message-ID: <000001c89e3a$3bb04a40$d00f7ca5@lurie.northwestern.edu> Dako rat anti-mouse. You'll need the rat secodaary. We run it LSAB. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michele Wich Sent: Friday, April 11, 2008 10:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ki-67 for mouse Can someone recommend a good Ki-67 antibody for use on mouse tissue? Thanks. This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mabosso <@t> unipathllc.com Mon Apr 14 09:42:19 2008 From: mabosso <@t> unipathllc.com (Mary Abosso) Date: Mon Apr 14 09:42:29 2008 Subject: [Histonet] VEGF Tissue Control Question Message-ID: <43A451981FF6634795BE83B1B5494D63136465@exchange.unipathllc.corp> Good Morning All - I am looking to see what human AP Labs are using for VEGF tissue controls, any help would be greatly appreciated. Mary Abosso UniPath LLC. Denver, CO From contact <@t> excaliburpathology.com Mon Apr 14 10:25:17 2008 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Mon Apr 14 10:25:23 2008 Subject: [Histonet] VEGF Tissue Control Question Message-ID: <128757.49619.qm@web50108.mail.re2.yahoo.com> Try pancreas. Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 631 N. Broadway Ave. Moore, OK 73160 405-759-3953 lab contact@excaliburpathology.com www.excaliburpathology.com From histo20 <@t> hotmail.com Mon Apr 14 10:32:46 2008 From: histo20 <@t> hotmail.com (Paula Wilder) Date: Mon Apr 14 10:32:52 2008 Subject: [Histonet] 40 Hour flex Message-ID: St. Joseph Medical Center in Towson, Maryland has a 40 hour daytime flex position available. Anyone interested please contact the Histology Supervisor at 410-337-1741. Thank you! _________________________________________________________________ Use video conversation to talk face-to-face with Windows Live Messenger. http://www.windowslive.com/messenger/connect_your_way.html?ocid=TXT_TAGLM_WL_Refresh_messenger_video_042008 From jqb7 <@t> cdc.gov Mon Apr 14 12:44:12 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Mon Apr 14 12:44:35 2008 Subject: [Histonet] Marker help Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A747A1D3@LTA3VS011.ees.hhs.gov> Hello everyone, I would like to know who of you out there are successfully doing IHC testing for CD4, 8, 20 and 68 on FFPE tissues. If you could provide me with the names of the companies where you purchase your antibodies I would greatly appreciate it. Thank you for your help! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov From TillRenee <@t> uams.edu Mon Apr 14 12:55:04 2008 From: TillRenee <@t> uams.edu (Till, Renee) Date: Mon Apr 14 12:55:09 2008 Subject: [Histonet] Choosing an antibody Message-ID: <11F927674DEBDC43B960809A7403C5D2083196E8@MAILPED.ad.uams.edu> I am searching for an F4/80 antibody to used on FFPE rat livers. I have found two possibilities (though if anyone happens to have an antibody suggestion please let me know) and I am wondering which would be better, or if it makes much difference. They are both from the same company but the sources differ. One is a rabbit polyclonal raised against amino acids w/in and extracellular domain, and the other is raised against a peptide mapping at the C-terminus. I have encountered this before and always wondered what is the difference and if one is better. Thanks. Renee' Till, HT (ASCP) Research Assistant Arkansas Children's Nutrition Center Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From liz <@t> premierlab.com Mon Apr 14 13:05:34 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Mon Apr 14 13:05:42 2008 Subject: [Histonet] Choosing an antibody In-Reply-To: <11F927674DEBDC43B960809A7403C5D2083196E8@MAILPED.ad.uams.edu> References: <11F927674DEBDC43B960809A7403C5D2083196E8@MAILPED.ad.uams.edu> Message-ID: Renee F4/80 does not work on rat, it's a marker for activated macrophages in mouse tisse. For rat you want the clone ED-1. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Till, Renee Sent: Monday, April 14, 2008 11:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Choosing an antibody I am searching for an F4/80 antibody to used on FFPE rat livers. I have found two possibilities (though if anyone happens to have an antibody suggestion please let me know) and I am wondering which would be better, or if it makes much difference. They are both from the same company but the sources differ. One is a rabbit polyclonal raised against amino acids w/in and extracellular domain, and the other is raised against a peptide mapping at the C-terminus. I have encountered this before and always wondered what is the difference and if one is better. Thanks. Renee' Till, HT (ASCP) Research Assistant Arkansas Children's Nutrition Center Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From March <@t> bio.fiocruz.br Mon Apr 14 13:09:53 2008 From: March <@t> bio.fiocruz.br (Renato Marchevsky) Date: Mon Apr 14 13:14:55 2008 Subject: [Histonet]gallocyanin Message-ID: <13BE6FF36818144CB18892951EBC586E0342D8CF@biocor01.biomanguinhos.br> Dear colleagues, I have been working hard to improve the CNS sections. I am most interested in getting information to improve our paraffin sections and gallocyanin staining from central nervous system of rhesus monkeys. Thanks, Renato S. Marchevsky Tecnologista Senior Laborat?rio de Neurovirul?ncia-Vice-Diretoria de Qualidade Bio-Manguinhos/ Fiocruz Tel: (21) 3882-9357 From rjbuesa <@t> yahoo.com Mon Apr 14 14:38:51 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Apr 14 14:38:57 2008 Subject: [Histonet] Marker help In-Reply-To: <1CE1847DFEA0A647B1CCDE4108EA60A747A1D3@LTA3VS011.ees.hhs.gov> Message-ID: <219473.24051.qm@web65714.mail.ac4.yahoo.com> NOVOCASTRA (= Leica Microsystems) for CD 4 and CD8 (HIER at pH8) and DAKO for CD20 and CD68 (HIER pH6) Ren? J. "Bartlett, Jeanine (CDC/CCID/NCZVED)" wrote: From afleming <@t> mednet.ucla.edu Mon Apr 14 15:30:09 2008 From: afleming <@t> mednet.ucla.edu (Alice Fleming) Date: Mon Apr 14 15:30:13 2008 Subject: [Histonet] Leydig cell marker? Message-ID: <49DE0F93-EB0B-4D37-AF0F-C23870558665@mednet.ucla.edu> Does anyone have a recommendation for Leydig cell markers for IHC in FFPE mouse tissue? Thanks very much! Alice Alice Fleming, PhD Department of Human Genetics Gonda Building 5524 University of California Los Angeles Los Angeles, CA 90095 310-267-2456 ---------------------------------------------------------- IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the recipient, please immediately notify us by return email, and delete this message from your computer. ---------------------------------------------------------- From LSebree <@t> uwhealth.org Mon Apr 14 15:56:42 2008 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Mon Apr 14 15:56:47 2008 Subject: [Histonet] Herpes Type 6 Message-ID: Good afternoon, One of our pathologists is looking for a reference lab that performs Herpes Simplex Virus Type 6 on FFPE human tissue. Thanks for any and all responses. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From gayle.callis <@t> bresnan.net Mon Apr 14 16:28:45 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Mon Apr 14 16:28:41 2008 Subject: [Histonet] Choosing an antibody References: <11F927674DEBDC43B960809A7403C5D2083196E8@MAILPED.ad.uams.edu> Message-ID: <001e01c89e76$85398cd0$6501a8c0@DHXTS541> Renee, This antibody is available from Serotec and spleen makes a good positive control. There are photos available on websites to show the interesting pattern of these cells in the rat spleen. We always detect with goat antimouse IgG isotype (whatever this is) -biotinylated, from Southern Biotechnology, and get no background. Gayle M. Callis HTL/HT/MT(ASCP) ----- Original Message ----- From: "Liz Chlipala" To: "Till, Renee" ; Sent: Monday, April 14, 2008 12:05 PM Subject: RE: [Histonet] Choosing an antibody Renee F4/80 does not work on rat, it's a marker for activated macrophages in mouse tisse. For rat you want the clone ED-1. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Till, Renee Sent: Monday, April 14, 2008 11:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Choosing an antibody I am searching for an F4/80 antibody to used on FFPE rat livers. I have found two possibilities (though if anyone happens to have an antibody suggestion please let me know) and I am wondering which would be better, or if it makes much difference. They are both from the same company but the sources differ. One is a rabbit polyclonal raised against amino acids w/in and extracellular domain, and the other is raised against a peptide mapping at the C-terminus. I have encountered this before and always wondered what is the difference and if one is better. Thanks. Renee' Till, HT (ASCP) Research Assistant Arkansas Children's Nutrition Center Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Mon Apr 14 18:31:02 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Mon Apr 14 18:31:02 2008 Subject: [Histonet] Choosing an antibody References: <11F927674DEBDC43B960809A7403C5D2083196E8@MAILPED.ad.uams.edu> <001e01c89e76$85398cd0$6501a8c0@DHXTS541> Message-ID: <000d01c89e87$9b03cbf0$6501a8c0@DHXTS541> Hi Liz, There is a whole set of rat macrophage antibodies, ED1, ED2 and ED3 from Serotec and it will depend on which subset one wants to see or as compared to a murine F4/80. We use frozen sections and always a secondary that recognizes the mouse IgG isotype from Southern Biotechnology. These antibodies are potent, and we use them at 1:1000 to 1:3000 depending on which one is needed. Be sure to determine working concentration with a dilution panel. I am sure these will work on FFPE tissue but do not have a recommendation for retrieval, since we never do that. They have really interesting and distinct patterns in the rat spleen for each subset, fun to do and certainly candidates for some gorgeous double staining! Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Liz Chlipala" To: "Gayle Callis" Sent: Monday, April 14, 2008 4:05 PM Subject: RE: [Histonet] Choosing an antibody Hey thanks for the update, I did not know that there was one the worked in rat. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com From joost.bruijntjes <@t> tno.nl Tue Apr 15 03:15:45 2008 From: joost.bruijntjes <@t> tno.nl (Bruijntjes, J.P. (Joost)) Date: Tue Apr 15 03:15:54 2008 Subject: [Histonet] (no subject) Message-ID: <8865601DD17A554CB489C17FFD8A51B2011D7692@MAIL04.tsn.tno.nl> Hi histonetters Is anyone of you familiar with the possibility to visualize peroxisome proliferation in FFPE (liver) tissues? Joost Bruijntjes TNO Quality of Life Zeist The Netherlands TNO.NL Joost Bruijntjes T +31 30 694 44 80 F +31 30 694 49 86 E joost.bruijntjes@tno.nl Disclaimer This e-mail and its contents are subject to the DISCLAIMER at http://www.tno.nl/disclaimer/email.html From olivier.leroux <@t> ugent.be Tue Apr 15 03:41:13 2008 From: olivier.leroux <@t> ugent.be (Olivier Leroux) Date: Tue Apr 15 03:42:37 2008 Subject: [Histonet] Acridine red Message-ID: <20080415104113.hev728p94wskwcw0@webmail.ugent.be> Dear all, I'm searching a company that supplies acridine red. Some articles mention Chroma (Stuttgart or k?ngen) as supplier, but I cannot find their website... best regards, Olivier Leroux -- Olivier Leroux Ghent University Department of Biology - Pteridological Section K.L. Ledeganckstraat 35 B-9000 Belgium http://www.pteridology.ugent.be From godsgalnow <@t> aol.com Tue Apr 15 07:47:45 2008 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Tue Apr 15 07:47:56 2008 Subject: [Histonet] specimen labels Message-ID: <8CA6D0E095E49FA-8B4-CD2@WEBMAIL-DF06.sysops.aol.com> Hello everyone.... We are thinking of getting custom labels for our specimen bottles.? Right now they all have a place for Pt Name, Dr Name and collection date.? We are thinking of changing it to a more unique label to include Pt Name, Requisition #, and Date..... What is everyone else doing? Roxanne From gu.lang <@t> gmx.at Tue Apr 15 09:03:26 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Apr 15 09:03:38 2008 Subject: [Histonet] recommendation for skin and biopsy-processing Message-ID: <000001c89f01$7aa868a0$eeeea8c0@dielangs.at> Hi, I want to implement a new processing protocol on the VIP 5 for biopsies and skin. Please tell me your comments, if the protocol would be sufficient for eg. 100 cassettes with biopsy-sponges and fifty cassettes with skin-specimen. The program starts at 11 p.m., the specimen sit in NBF from afternoon till the start. 30 min NBF - 30 min NBF - 30 min 70% - 30 min 80% - 30 min 96% - 40 min 96% - 40 min 100% - 40 min 100% - 30 min shell sol (clearing medium) - 40 min shell sol - 30 min Paraffin - 30 min paraffin - 40 min paraffin - 40 min paraffin. Until now we process these specimen with our standard (13 hours) protocol and have no troubles with them. But I want to extend and improve fixation. Thanks Gudrun Lang From MElliott <@t> mrl.ubc.ca Tue Apr 15 09:08:30 2008 From: MElliott <@t> mrl.ubc.ca (Mark Elliott) Date: Tue Apr 15 09:08:57 2008 Subject: [Histonet] Region IX Education Day June 6/7 Moncton NB Message-ID: <4804546E.11C6.00D6.0@mrl.ubc.ca> Region IX is hosting their next Education Day June 6th and 7th in beautiful Moncton, New Brunswick. The event starts Friday evening with a Wine and Cheese reception with the vendors. Saturday consists of 5 different lectures, and the vendors will be in attendance as well with displays. Further information, and registration forms can be obtained from the Region IX Website (http://www.nshregionix.org/education.html) or from myself. A block of hotel rooms is being held at the Ramada Crystal Plaza Palace Hotel until May 6th so book early. Pre-registration for the event is required and the deadline is May 23, 2008. Hope to see you there. Mark Elliott Region IX Education Committee Chair melliott@mrl.ubc.ca ***CONFIDENTIALITY NOTICE*** This electronic message is intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From TownsendD <@t> childrensdayton.org Tue Apr 15 09:14:51 2008 From: TownsendD <@t> childrensdayton.org (Dolores Townsend) Date: Tue Apr 15 09:15:30 2008 Subject: [Histonet] Cleveland HSO Message-ID: I will be going to the HSO convention in Cleveland, OH this coming Friday and Saturday. Anyone else from the Histonet going? It would be fun to meet some of the great people on the list. Dolores From jdooley2008 <@t> yahoo.com Tue Apr 15 09:43:47 2008 From: jdooley2008 <@t> yahoo.com (James Dooley) Date: Tue Apr 15 09:43:56 2008 Subject: [Histonet] apoptosis Message-ID: <489078.75878.qm@web45916.mail.sp1.yahoo.com> Hello, I am looking for a Ki-67 or other antibody for marking apoptotic cells in mouse. I would like to use fresh frozen tissue embedded in OCT. Thank you, James ____________________________________________________________________________________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ From marktarango <@t> gmail.com Tue Apr 15 10:52:55 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Tue Apr 15 10:53:00 2008 Subject: [Histonet] apoptosis In-Reply-To: <489078.75878.qm@web45916.mail.sp1.yahoo.com> References: <489078.75878.qm@web45916.mail.sp1.yahoo.com> Message-ID: <5b6eb13e0804150852m158bb426y1ddf0ce2f04e9b5b@mail.gmail.com> If you want to stain apoptotic cells, don't use ki67. Ki67 will stain the dividing cells. Try caspase 3 or something else for apopotic cells. On Tue, Apr 15, 2008 at 7:43 AM, James Dooley wrote: > Hello, > I am looking for a Ki-67 or other antibody for marking apoptotic cells in > mouse. I would like to use fresh frozen tissue embedded in OCT. > Thank you, > James > > > > > > ____________________________________________________________________________________ > Be a better friend, newshound, and > know-it-all with Yahoo! Mobile. Try it now. > http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jwatson <@t> gnf.org Tue Apr 15 10:54:46 2008 From: jwatson <@t> gnf.org (James Watson) Date: Tue Apr 15 10:54:55 2008 Subject: [Histonet] Acridine red In-Reply-To: <20080415104113.hev728p94wskwcw0@webmail.ugent.be> References: <20080415104113.hev728p94wskwcw0@webmail.ugent.be> Message-ID: Try this http://www.chroma.de/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Olivier Leroux Sent: Tuesday, April 15, 2008 1:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Acridine red Dear all, I'm searching a company that supplies acridine red. Some articles mention Chroma (Stuttgart or k?ngen) as supplier, but I cannot find their website... best regards, Olivier Leroux -- Olivier Leroux Ghent University Department of Biology - Pteridological Section K.L. Ledeganckstraat 35 B-9000 Belgium http://www.pteridology.ugent.be _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bdelescavage <@t> cellnetix.com Tue Apr 15 11:04:31 2008 From: bdelescavage <@t> cellnetix.com (Beth Delescavage) Date: Tue Apr 15 11:10:02 2008 Subject: [Histonet] Thanks for all of the CMV help Message-ID: Hi Everyone~ I cannot thank you all enough for all of help you gave me finding CMV controls. Thanks again!!! Beth Beth Delescavage, BS, HTL (ASCP) QIHC CellNetix Labs DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From rjbuesa <@t> yahoo.com Tue Apr 15 11:19:12 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Apr 15 11:19:16 2008 Subject: [Histonet] recommendation for skin and biopsy-processing In-Reply-To: <000001c89f01$7aa868a0$eeeea8c0@dielangs.at> Message-ID: <215702.56592.qm@web65715.mail.ac4.yahoo.com> With such a period in NBF before starting the protocol, you can skip the second NBF station, and add at the end another white spirit station to clear the specimens better. Ren? J. Gudrun Lang wrote: Hi, I want to implement a new processing protocol on the VIP 5 for biopsies and skin. Please tell me your comments, if the protocol would be sufficient for eg. 100 cassettes with biopsy-sponges and fifty cassettes with skin-specimen. The program starts at 11 p.m., the specimen sit in NBF from afternoon till the start. 30 min NBF - 30 min NBF - 30 min 70% - 30 min 80% - 30 min 96% - 40 min 96% - 40 min 100% - 40 min 100% - 30 min shell sol (clearing medium) - 40 min shell sol - 30 min Paraffin - 30 min paraffin - 40 min paraffin - 40 min paraffin. Until now we process these specimen with our standard (13 hours) protocol and have no troubles with them. But I want to extend and improve fixation. Thanks Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet between 0000-00-00 and 9999-99-99 From Erin.Martin <@t> ucsf.edu Tue Apr 15 11:45:23 2008 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Tue Apr 15 11:50:12 2008 Subject: [Histonet] No Tox and immuno Message-ID: Hi everyone, Does anyone have any experience with NoToxHisto formalin substitute and immuno? We occassionally recieve specimens that were fixed in it and I am not sure if I need to handle the IHC differently. The company web site says that it does not require any changes to processing or staining protocols, but... Did you have to change anything, possibly your retrieval techniques? Thank you in advance, Erin M From alonso.martinezcanabal <@t> utoronto.ca Tue Apr 15 12:07:20 2008 From: alonso.martinezcanabal <@t> utoronto.ca (Alonso Martinez-Canabal) Date: Tue Apr 15 12:07:47 2008 Subject: [Histonet] apoptosis In-Reply-To: <489078.75878.qm@web45916.mail.sp1.yahoo.com> References: <489078.75878.qm@web45916.mail.sp1.yahoo.com> Message-ID: Ki-67 as an apoptotic marker? That is a little bit extrange to me. I heard that some cells before dying restart the cell cycle but that is not clear. The most common and accepted apoptosis marker in antibody, is caspase-3-cleaved. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James Dooley Sent: April-15-08 10:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] apoptosis Hello, I am looking for a Ki-67 or other antibody for marking apoptotic cells in mouse. I would like to use fresh frozen tissue embedded in OCT. Thank you, James ____________________________________________________________________________ ________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From holly.valentine <@t> Vanderbilt.Edu Tue Apr 15 12:07:06 2008 From: holly.valentine <@t> Vanderbilt.Edu (Valentine, Holly) Date: Tue Apr 15 12:11:32 2008 Subject: [Histonet] Calibrating a Reichert Histostat 820 References: Message-ID: Dear members: Our laboratory has recently acquired an old Reichert Histostat 820 which we intend to use to begin cutting our own paraffin sections. However it does need to be recalibrated. Do any members know of histology instrument technicians who could do this for us at a reasonable price? Thank you. Holly Valentine DVM Research Instructor Vanderbilt University Medical Center Nashville, TN From kmilne <@t> bccancer.bc.ca Tue Apr 15 12:13:38 2008 From: kmilne <@t> bccancer.bc.ca (Milne, Katy) Date: Tue Apr 15 12:13:48 2008 Subject: [Histonet] RE: Marker Help In-Reply-To: Message-ID: <07979E76B0869D4E8C9FE4AA9FC0657804668F80@srvex03.phsabc.ehcnet.ca> Hi Jeanine, I'm assuming this is for human tissue? CD4 - I have from Lab Vision (Thermo), it's ok but you have to use it more concentrated so I find I get some background - Cat # MS-1528 CD8 - I have a Rb Mab also from Lab Vision, it's great Cat# RM-9116 CD20 - Also from Lab Vision, it's really great too RB-9013 (Rb Pab) CD68 - Again, Lab Vision - MS-1808, works quite well I can send you pictures of any of the markers if you need. Katy Milne Deeley Research Centre Message: 1 Date: Mon, 14 Apr 2008 13:44:12 -0400 From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" Subject: [Histonet] Marker help To: histonet Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A747A1D3@LTA3VS011.ees.hhs.gov> Content-Type: text/plain; charset=us-ascii Hello everyone, I would like to know who of you out there are successfully doing IHC testing for CD4, 8, 20 and 68 on FFPE tissues. If you could provide me with the names of the companies where you purchase your antibodies I would greatly appreciate it. Thank you for your help! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov From jwray78 <@t> gmail.com Tue Apr 15 12:22:32 2008 From: jwray78 <@t> gmail.com (Josh Wray) Date: Tue Apr 15 12:22:43 2008 Subject: [Histonet] Thanks for the PAS/D info Message-ID: <883927cf0804151022u58cbaaddj20dd960e2fd04431@mail.gmail.com> I appreciate all of the info regarding the digestion issue of the PAS/D. I will pass it along to our Histology Dept. Josh Wray HT(ASCP) Esoterics Laboratory Ameripath-Indianapolis From lblazek <@t> digestivespecialists.com Tue Apr 15 12:30:50 2008 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Apr 15 12:26:36 2008 Subject: [Histonet] Calibrating a Reichert Histostat 820 In-Reply-To: References: Message-ID: <1F937FB30BDB7C4A9F39F83FEA8D379F9F53DF@bruexchange1.digestivespecialists.com> Holly, Try Marston Technical Service, Inc. in Cincinnati OH. 1 800 966-1020. They have been great in service for me for a long time. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Valentine, Holly Sent: Tuesday, April 15, 2008 1:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Calibrating a Reichert Histostat 820 Dear members: Our laboratory has recently acquired an old Reichert Histostat 820 which we intend to use to begin cutting our own paraffin sections. However it does need to be recalibrated. Do any members know of histology instrument technicians who could do this for us at a reasonable price? Thank you. Holly Valentine DVM Research Instructor Vanderbilt University Medical Center Nashville, TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dbarkmei <@t> med.wayne.edu Tue Apr 15 13:08:33 2008 From: dbarkmei <@t> med.wayne.edu (Dan Barkmeier) Date: Tue Apr 15 13:08:41 2008 Subject: [Histonet] Frozen rat brains sections: OCT adheres, tissue does not Message-ID: <8a577a7f0804151108h7cd92bf7k8a1f7acd9f26a7d4@mail.gmail.com> I've experienced ongoing frustrations with cryosectioning rat brains for over a year now, and felt it was time to seek professional help. I'm having trouble getting good quality sections, which seems to stem from a varying mix of these two related problems: 1) Upon cutting, parts of the tissue section detach from the surrounding OCT embedding medium 2) When adhering the section to a slide, the OCT sticks immediately, but the section is not very adherent. The causes the perimeter of OCT to 'run' around the section well before the neighboring tissue is stuck, and I get giant wrinkles in the section. Tissue processing: - Quickly dissect brains, cut them into 2-5mm coronal slices, and place them in 4% paraformaldehyde in PBS for 48 hours - Cryoprotect in 30% sucrose in PBS until tissue sinks (1-2 days) - Embed in OCT on dry ice - Cut sections at 20um (I've tried 10um as well, with similar results), and at various temperatures from -17C to -22C, and mount on SuperFrost Plus slides I don't really have problems cutting human brain samples prepared in the same manner; it's just my rats that give me trouble. I would greatly appreciate any advice offered. I've searched through the archives as best I can, but I did not find these problems specifically addressed. - Dan Barkmeier Wayne State University School of Medicine From ljones <@t> pathology.umsmed.edu Tue Apr 15 13:32:05 2008 From: ljones <@t> pathology.umsmed.edu (Linda Jones) Date: Tue Apr 15 13:34:10 2008 Subject: [Histonet] Employment in Cleveland Mississippi Message-ID: <4804AE550200004400022B6D@GWIA1.umsmed.edu> Hi everyone, If anyone is interested in employment in Cleveland Mississippi from 4:00a-12:30 Monday thru Friday, please call: Marie Sparacino Bolivar Pathology Services Phone number (601)573-2307 Fax number (662)846-5684 E-mail bolivarpath@yahoo.com or Linda Harper-Jones BS.,HT/ HTL(ASCP) University of Mississippi Medical Center Department of Pathology Chief Histotechnologist Supervisor (601) 984-1576 (601) 984-4968 Fax ljones@pathology.umsmed.edu Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. From schaundrawalton <@t> yahoo.com Tue Apr 15 14:17:56 2008 From: schaundrawalton <@t> yahoo.com (Schaundra Walton) Date: Tue Apr 15 14:17:59 2008 Subject: [Histonet] Re: Marker Help Message-ID: <636235.61365.qm@web58902.mail.re1.yahoo.com> We use Dako for our CD20. Schaundra Walton BS, HTL(ASCP) Histology Supervisor Swedish American Hospital Rockford, IL ----------------------------------------Original Message-------------------------------------- Hello everyone, I would like to know who of you out there are successfully doing IHC testing for CD4, 8, 20 and 68 on FFPE tissues. If you could provide me with the names of the companies where you purchase your antibodies I would greatly appreciate it. Thank you for your help! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov between 0000-00-00 and 9999-99-99 From mary.gessford <@t> spcorp.com Tue Apr 15 14:29:47 2008 From: mary.gessford <@t> spcorp.com (Gessford, Mary) Date: Tue Apr 15 14:29:59 2008 Subject: [Histonet] Ihc for Helicobacter; IHC for myoglobulin on Rodents In-Reply-To: <07979E76B0869D4E8C9FE4AA9FC0657804668F80@srvex03.phsabc.ehcnet.ca> Message-ID: Hello everyone, One of my Pathologist wanted me to ask: Please ask them if they have the experience running IHC for Helicobacter pylori from DAKO-rabbit (probably polyclonal). Does it cross react with other spiral-like bacteria such as Campylobacter sp., Helicobacter sp., Lawsonia intracellularis, Brachyspira spp? Would you ask IHC for myoglobulin in Rodents? Where do they get it from? Ingrid DR.Pardo D.V.M., MS., Diplomate A.C.V.P. Chair, Latin Comparative Pathology Group Senior Research Pathologist Schering-Plough Research Institute ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From fudo <@t> ufl.edu Tue Apr 15 15:21:59 2008 From: fudo <@t> ufl.edu (FU,DONGTAO) Date: Tue Apr 15 15:22:02 2008 Subject: [Histonet] background of H&E staining Message-ID: <1883786817.108101208290919038.JavaMail.osg@osgjas03.cns.ufl.edu> Hi, all Our PI complained about our H&E staining recently. He found the slides we did for him changed color(within a month), the whole slides turn to yellow, although the staining is still there and looks good. I searched for some websites for the answer, but could not find it. Does anyone know the reason why the background of slides changed color? We did it in the general way which worked well in the past. And the reagents are not expired. Any suggestions? Thanks, Ann From SDrew <@t> uwhealth.org Tue Apr 15 15:46:43 2008 From: SDrew <@t> uwhealth.org (Drew Sally A.) Date: Tue Apr 15 15:46:48 2008 Subject: [Histonet] EMA and meningiomas Message-ID: <3F328377AF4E23438E78234752652CE105D526C2@uwhis-xchng7.uwhis.hosp.wisc.edu> Does anyone have a feel for any particular clone of EMA that works best on meningiomas? We run our immunos on the Ventana platforms... Thank you for any and all opinions on EMA clones! Sally Ann Drew, MT(ASCP) IHC/ISH Laboratory University of Wisconsin Hosp. & Clinics Madison, WI 53792 (608)265-6596 From KatieG <@t> alleninstitute.org Tue Apr 15 18:35:18 2008 From: KatieG <@t> alleninstitute.org (Katie Glattfelder) Date: Tue Apr 15 18:35:22 2008 Subject: [Histonet] Frozen rat brains sections: OCT adheres, tissue does not In-Reply-To: <8a577a7f0804151108h7cd92bf7k8a1f7acd9f26a7d4@mail.gmail.com> References: <8a577a7f0804151108h7cd92bf7k8a1f7acd9f26a7d4@mail.gmail.com> Message-ID: I cut fresh frozen mouse brains at 25 microns for about two years and we frequently encountered the same issue. There are a couple of things you can try: 1) try warming up your temperature a little bit - we usually found that an object temp of -11 to -13 and a chamber temp of -14 to -16 worked best 2) try cooling your rollplate immediately before taking a section, sometimes this can reduce the separation but often has to be done frequently 3) if you haven't already, try trimming your OCT fairly close (1-2 mm?) at the edge that touches the blade first and try to catch the first part of the section at the same time or close to the same time as the OCT, then tilt the slide down quickly to get the whole section without wrinkles or folds (it's hard to describe a technique with words!) 4) try using Neg-50 instead of OCT. Neg-50 greatly reduced tissue separating from the embedding media but takes some getting used to because it "behaves" differently than OCT. If you use NEG 50, you should reduce your temperatures by 2-3 degrees from what I recommended above, and probably have some practice tissue to get used to it. Hope this helps! Katie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dan Barkmeier Sent: Tuesday, April 15, 2008 11:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen rat brains sections: OCT adheres, tissue does not I've experienced ongoing frustrations with cryosectioning rat brains for over a year now, and felt it was time to seek professional help. I'm having trouble getting good quality sections, which seems to stem from a varying mix of these two related problems: 1) Upon cutting, parts of the tissue section detach from the surrounding OCT embedding medium 2) When adhering the section to a slide, the OCT sticks immediately, but the section is not very adherent. The causes the perimeter of OCT to 'run' around the section well before the neighboring tissue is stuck, and I get giant wrinkles in the section. Tissue processing: - Quickly dissect brains, cut them into 2-5mm coronal slices, and place them in 4% paraformaldehyde in PBS for 48 hours - Cryoprotect in 30% sucrose in PBS until tissue sinks (1-2 days) - Embed in OCT on dry ice - Cut sections at 20um (I've tried 10um as well, with similar results), and at various temperatures from -17C to -22C, and mount on SuperFrost Plus slides I don't really have problems cutting human brain samples prepared in the same manner; it's just my rats that give me trouble. I would greatly appreciate any advice offered. I've searched through the archives as best I can, but I did not find these problems specifically addressed. - Dan Barkmeier Wayne State University School of Medicine _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pieronelva01 <@t> bigpond.com Wed Apr 16 06:37:59 2008 From: pieronelva01 <@t> bigpond.com (Piero Nelva) Date: Wed Apr 16 06:38:10 2008 Subject: [Histonet] background of H&E staining References: <1883786817.108101208290919038.JavaMail.osg@osgjas03.cns.ufl.edu> Message-ID: <003a01c89fb6$529a0930$6371be7c@pentium4> We had a batch of mounting medium that went yellow, and consequently the section went yellow too (so long ago that I've forgotten the brand). Since switching to Eukitt, the preparation is much more stable. Piero Nelva Histology Monash Medical Centre Australia ----- Original Message ----- From: "FU,DONGTAO" To: Sent: Wednesday, April 16, 2008 6:21 AM Subject: [Histonet] background of H&E staining > Hi, all > > Our PI complained about our H&E staining recently. He found the slides we > did for him changed color(within a month), the whole slides turn to > yellow, although the staining is still there and looks good. I searched > for some websites for the answer, but could not find it. > > Does anyone know the reason why the background of slides changed color? > We did it in the general way which worked well in the past. And the > reagents are not expired. Any suggestions? > > Thanks, > > Ann > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG. Version: 7.5.524 / Virus Database: 269.23.0/1381 - Release > Date: 4/16/2008 9:34 AM > > From Instaken <@t> aol.com Wed Apr 16 06:58:05 2008 From: Instaken <@t> aol.com (Instaken@aol.com) Date: Wed Apr 16 06:58:31 2008 Subject: [Histonet] agencies for temporary histotechs Message-ID: Anyone have company names and contact info representing histotechs for temporary positions? Many thanks, Ken **************It's Tax Time! Get tips, forms and advice on AOL Money & Finance. (http://money.aol.com/tax?NCID=aolcmp00300000002850) From ktalbot <@t> jhmi.edu Wed Apr 16 07:07:11 2008 From: ktalbot <@t> jhmi.edu (Karen Fox-Talbot) Date: Wed Apr 16 07:07:22 2008 Subject: [Histonet] Myoglobin Message-ID: <4805B27B.FB24.0071.0@jhmi.edu> Can anyone recommend an antibody to myoglobin that works in FFPE mouse hearts? Karen From rjbuesa <@t> yahoo.com Wed Apr 16 08:47:11 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Apr 16 08:47:18 2008 Subject: [Histonet] background of H&E staining In-Reply-To: <1883786817.108101208290919038.JavaMail.osg@osgjas03.cns.ufl.edu> Message-ID: <41576.75306.qm@web65716.mail.ac4.yahoo.com> This usually happens when washing is incomplete and some residual acid pH remains on the section that causes the fading over some time. Ren? J. "FU,DONGTAO" wrote: Hi, all Our PI complained about our H&E staining recently. He found the slides we did for him changed color(within a month), the whole slides turn to yellow, although the staining is still there and looks good. I searched for some websites for the answer, but could not find it. Does anyone know the reason why the background of slides changed color? We did it in the general way which worked well in the past. And the reagents are not expired. Any suggestions? Thanks, Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From fudo <@t> ufl.edu Wed Apr 16 08:49:22 2008 From: fudo <@t> ufl.edu (FU,DONGTAO) Date: Wed Apr 16 08:49:33 2008 Subject: [Histonet] background of H&E staining Message-ID: <1286915206.187661208353762515.JavaMail.osg@osgjas04.cns.ufl.edu> Thank you all for answering my question. I think I need to change our mounting medium first to see whether it helps. Have a nice day! Ann On Wed Apr 16 07:37:59 EDT 2008, Piero Nelva wrote: > We had a batch of mounting medium that went yellow, and > consequently the section went yellow too (so long ago that I've > forgotten the brand). Since switching to Eukitt, the preparation > is much more stable. > > Piero Nelva > Histology > Monash Medical Centre > Australia > ----- Original Message ----- From: "FU,DONGTAO" > To: > Sent: Wednesday, April 16, 2008 6:21 AM > Subject: [Histonet] background of H&E staining > > >> Hi, all >> >> Our PI complained about our H&E staining recently. He found the >> slides we did for him changed color(within a month), the whole >> slides turn to yellow, although the staining is still there and >> looks good. I searched for some websites for the answer, but >> could not find it. >> >> Does anyone know the reason why the background of slides >> changed color? We did it in the general way which worked well in >> the past. And the reagents are not expired. Any suggestions? >> >> Thanks, >> >> Ann >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> -- No virus found in this incoming message. >> Checked by AVG. Version: 7.5.524 / Virus Database: 269.23.0/1381 >> - Release Date: 4/16/2008 9:34 AM >> >> > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Ann Dongtao Fu MD, Ph.D Lab Manager Dept. of Pathology Lab phone: 352-273-7752 Lab FAX: 352-273-7755 Lab address: D11-50 PO Box: 100275 1600 SW Archer Road University of Flodrida Gainesville, FL 32610 From jdooley2008 <@t> yahoo.com Wed Apr 16 09:15:19 2008 From: jdooley2008 <@t> yahoo.com (James Dooley) Date: Wed Apr 16 09:15:33 2008 Subject: [Histonet] Ki-67 and Caspase 3 Message-ID: <179357.26342.qm@web45903.mail.sp1.yahoo.com> I would like to measure both proliferation and apoptosis. Can anyone recommend a good Ki-67 antibody of use on fresh frozen tissue to measure proliferation and an antibody for activated caspase 3 for apoptosis on mouse tissue. Please not fixation used if possible. I apologize for the confusion with my previous e-mail. Thank you, James ____________________________________________________________________________________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ From marktarango <@t> gmail.com Wed Apr 16 09:23:19 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Apr 16 09:23:27 2008 Subject: [Histonet] Ki-67 and Caspase 3 In-Reply-To: <179357.26342.qm@web45903.mail.sp1.yahoo.com> References: <179357.26342.qm@web45903.mail.sp1.yahoo.com> Message-ID: <5b6eb13e0804160723i5dab6a28lffc87ba8063cc160@mail.gmail.com> Dako has a rat anti-mouse ki67 that works. I've used it before. It doesn't cross-react with human. On Wed, Apr 16, 2008 at 7:15 AM, James Dooley wrote: > I would like to measure both proliferation and apoptosis. Can anyone > recommend a good Ki-67 antibody of use on fresh frozen tissue to measure > proliferation and an antibody for activated caspase 3 for apoptosis on mouse > tissue. Please not fixation used if possible. I apologize for the confusion > with my previous e-mail. > Thank you, > James > > > > > > ____________________________________________________________________________________ > Be a better friend, newshound, and > know-it-all with Yahoo! Mobile. Try it now. > http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From SharonC <@t> celligent.net Wed Apr 16 09:38:14 2008 From: SharonC <@t> celligent.net (Sharon Campbell) Date: Wed Apr 16 09:35:57 2008 Subject: [Histonet] Thermofisher Microm 420 Processor Message-ID: Hello Histo world: Does anyone have any experience good or bad with the ThermoFisher Microm 420 rapid tissue processor? We are primarily a Derm lab but also process a few Gyn's and other surgical tissues. Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 (704) 549-8444 x100 sharonc@celligent.net From igor.deyneko <@t> gmail.com Wed Apr 16 09:44:47 2008 From: igor.deyneko <@t> gmail.com (Igor Deyneko) Date: Wed Apr 16 09:44:55 2008 Subject: [Histonet] Fixing & Processing Cell Pellets Message-ID: <35e16a770804160744g78c62844g59db1dc1574afe44@mail.gmail.com> Dear Histonetters! I was wondering if anyone knows or has a good protocol for processing, fixing, and embedding the pelleted cells. I was given a protocol by my boss, but I have a few questions about it. Protocol I got: Pellet cells, wash with PBS, re-pellet. Remove PBS and gently add 10%NBF. 10NBF-until fixed 6-24 hours. 70-85-95-95-100-100-100% Ethanols(15-20 minutes each). 3 xylene changes 20 minutes each. 4 paraffin changes 30 minutes each(melted paraffin is the first heated step 60 degrees). Embed and cut at 5 microns using a rotary microtome and accu edge blades, lay out on a 47 degree water bath, let dry on a flat warming table or in the oven and they are ready for deparaffinization. So I have questions as to how would i extract cellf from the test tube without disturbing the pellet? Then what could I put them into for dehydration with alcohols and clearance with xylene? As far as paraffin goes as well,are there any special cassettes designed for cells? Also as far as embedding goes, would I do the standard embedding or are there special techniques? I would appreciate any information/ protocols. Thank you in advance. Igor Deyneko Infinity Pharmaceuticals, Cambridge, MA From kadamsplw <@t> gmail.com Wed Apr 16 09:22:31 2008 From: kadamsplw <@t> gmail.com (karen adams) Date: Wed Apr 16 09:51:41 2008 Subject: [Histonet] Automated stainer problem Message-ID: Hello everyone....I am trying to bring a (refurbished) Leica XL automated stainer on line....I am using all Fischer reagents: Hematoxylin 7212, clarifier 2 and Eosin Y.....everything looks great except the cervical Bx...which my pathologist is saying looks "too blue" (no mucin) ...tonsils and other lymph tissue looks good, just Cx Bx....my eosin looks good with 3 shades...I am just not sure where to go next....any ideas?? Thanks so much ! -- Karen Adams Supervisor Pathology Laboratories West 9303 Park West Blvd. Knoxville, TN 37923 kadamsplw@gmail.com From HParker <@t> Skaggs.Net Wed Apr 16 09:53:04 2008 From: HParker <@t> Skaggs.Net (Parker, Helayne) Date: Wed Apr 16 09:53:08 2008 Subject: [Histonet] specimen labels In-Reply-To: <20080415171701.30CFC3B8115@barracuda.skaggs.net> Message-ID: <2FAF8CC41C5AAF43AAA52F26782E1A5602610E47@mail1-schc.skaggs.net> Specimen labels should have at least: Patient's name a 2nd identifier (i.e. DOB or medical record # or both if you like) JCAHO requires 2 patients identifiers to be on all bottles Additionally to make it nicer for you etc A) specimen site location B) Doctor/surgeon C) date of service Are all good additional info. Helayne Parker, HT (ASCP) Histology Section Head Skaggs Community Health Center Branson, Missouri From Allison_Scott <@t> hchd.tmc.edu Wed Apr 16 09:52:34 2008 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Wed Apr 16 09:55:15 2008 Subject: [Histonet] Disposal of formalin containers Message-ID: <1872B4A455B7974391609AD8034C79FC8BD41B@LBEXCH01.hchd.local> Hello to all in histoland. Our safety officer inquired If we had a procedure for the disposal of our formalin bottles. The only procedure that I had was for the waste formalin. We just throw the bottles in the trash for disposal. Does any one do anything special for this type of disposal. Your help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital From marktarango <@t> gmail.com Wed Apr 16 10:01:03 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Apr 16 10:01:13 2008 Subject: [Histonet] Disposal of formalin containers In-Reply-To: <1872B4A455B7974391609AD8034C79FC8BD41B@LBEXCH01.hchd.local> References: <1872B4A455B7974391609AD8034C79FC8BD41B@LBEXCH01.hchd.local> Message-ID: <5b6eb13e0804160801qa6f5428t9578bc9649eafc0d@mail.gmail.com> I wondering... can they could be recycled? On Wed, Apr 16, 2008 at 7:52 AM, Scott, Allison D < Allison_Scott@hchd.tmc.edu> wrote: > Hello to all in histoland. Our safety officer inquired If we had a > procedure for the disposal of our formalin bottles. The only procedure > that I had was for the waste formalin. We just throw the bottles in the > trash for disposal. Does any one do anything special for this type of > disposal. Your help in this matter will be greatly appreciated. > > Allison Scott HT(ASCP) > Histology Supervisor > LBJ Hospital > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From angela.mcnabola <@t> ikonisys.com Wed Apr 16 10:02:52 2008 From: angela.mcnabola <@t> ikonisys.com (Angela McNabola) Date: Wed Apr 16 10:02:56 2008 Subject: [Histonet] Fixing & Processing Cell Pellets In-Reply-To: <35e16a770804160744g78c62844g59db1dc1574afe44@mail.gmail.com> Message-ID: <4ECD18F12E443644B1F3C924A2039824839050@ikoexchange.ikonisys.com> We used to use Histo-gel from Richard Allen, it worked quite nicely, kept the pellet together and processed well. It didn't interfere with any IHC either. I'm sorry that I can not give you more specifics as I am currently in a new position without our protocol in from of me and do not want to give you any misinformation, but I believe that after the fixation, we followed the manufacturer's protocol. Here is the link: http://www.rallansci.com/histology/histology.aspx?id=2 Angela McNabola, MS HT(ASCP)SLS, QIHC Research Scientist Ikonisys, Inc. 5 Science Park New Haven, CT 06511 203-776-0791 angela.mcnabola@ikonisys.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Igor Deyneko Sent: Wednesday, April 16, 2008 10:45 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Fixing & Processing Cell Pellets Dear Histonetters! I was wondering if anyone knows or has a good protocol for processing, fixing, and embedding the pelleted cells. I was given a protocol by my boss, but I have a few questions about it. Protocol I got: Pellet cells, wash with PBS, re-pellet. Remove PBS and gently add 10%NBF. 10NBF-until fixed 6-24 hours. 70-85-95-95-100-100-100% Ethanols(15-20 minutes each). 3 xylene changes 20 minutes each. 4 paraffin changes 30 minutes each(melted paraffin is the first heated step 60 degrees). Embed and cut at 5 microns using a rotary microtome and accu edge blades, lay out on a 47 degree water bath, let dry on a flat warming table or in the oven and they are ready for deparaffinization. So I have questions as to how would i extract cellf from the test tube without disturbing the pellet? Then what could I put them into for dehydration with alcohols and clearance with xylene? As far as paraffin goes as well,are there any special cassettes designed for cells? Also as far as embedding goes, would I do the standard embedding or are there special techniques? I would appreciate any information/ protocols. Thank you in advance. Igor Deyneko Infinity Pharmaceuticals, Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From M.Walker <@t> hrsu.mrc.ac.uk Wed Apr 16 10:14:25 2008 From: M.Walker <@t> hrsu.mrc.ac.uk (Marion Walker) Date: Wed Apr 16 10:16:25 2008 Subject: [Histonet] Leydig cell marker? [Scanned] In-Reply-To: References: Message-ID: <86F334797DC6524A99AD9DD8F23A8B500DDDE4@mailserv.hrsu.mrc.ac.uk> Alice We have routinely used 3Beta-Hydroxy Steroid Dehydrogenase as a Leydig cell marker. We have recently had to source a new antibody and are currently using a goat anti human antibody from Santa Cruz sc-30820. We get very clean staining on both mouse and rat testis. Marion Walker MRC Human Reproductive Sciences Unit Queen's Medical Research Institute 47 Little France Crescent Edinburgh EH16 4TJ 0131 242 9154 ------------------------------ Message: 6 Date: Mon, 14 Apr 2008 13:30:09 -0700 From: Alice Fleming Subject: [Histonet] Leydig cell marker? To: Histonet@lists.utsouthwestern.edu Message-ID: <49DE0F93-EB0B-4D37-AF0F-C23870558665@mednet.ucla.edu> Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes Does anyone have a recommendation for Leydig cell markers for IHC in FFPE mouse tissue? Thanks very much! Alice Alice Fleming, PhD Department of Human Genetics Gonda Building 5524 University of California Los Angeles Los Angeles, CA 90095 310-267-2456 From Dorothy.L.Webb <@t> HealthPartners.Com Wed Apr 16 10:24:14 2008 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Wed Apr 16 10:24:25 2008 Subject: [Histonet] certification Message-ID: <0E394B648E5284478A6CCB78E5AFDA2705635627@hpes1.HealthPartners.int> I have a histotech who has been in the field for 9 years, went to school for histology in 1999, (2 year Associates), passed the practical portion of the registry, but did not take the written within the 5 year allowance. She recently sent her $175 in and all paperwork to register for taking the test and has been told by ASCP that she is no longer eligible to take the test due to her credits not being from an accredited school. The school she attended was accredited and then taken over a few years ago by a University out of Chicago, so is now under new management and name. The present college can only accept "X" number of credits for transfer, even though it is the same program, same person in charge of the program ,etc. And, to top it all off, ASCP will not refund her entry fee nor hold it for her when she can take the exam. I find this hard to understand when there are so few schools around and techs are hard to find. This person is a very good tech and did not take the registry exam until we forced her hand, due to her being a single mom of twins with no financial support, etc. Has anyone ever heard of such a scenario and any ideas to help this tech, other than her trying to retake several college credits? Thanks for any ideas or suggestions to help out a fellow histotech!!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From Jackie.O'Connor <@t> abbott.com Wed Apr 16 10:30:07 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed Apr 16 10:30:28 2008 Subject: [Histonet] Disposal of formalin containers In-Reply-To: <5b6eb13e0804160801qa6f5428t9578bc9649eafc0d@mail.gmail.com> Message-ID: I wouldn't think so - aldehydes may have leeched into the plastic. Would you buy a baby bottle made from recycled formalin containers? "Mark Tarango" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/16/2008 10:01 AM To "Scott, Allison D" cc histonet@lists.utsouthwestern.edu Subject Re: [Histonet] Disposal of formalin containers I wondering... can they could be recycled? On Wed, Apr 16, 2008 at 7:52 AM, Scott, Allison D < Allison_Scott@hchd.tmc.edu> wrote: > Hello to all in histoland. Our safety officer inquired If we had a > procedure for the disposal of our formalin bottles. The only procedure > that I had was for the waste formalin. We just throw the bottles in the > trash for disposal. Does any one do anything special for this type of > disposal. Your help in this matter will be greatly appreciated. > > Allison Scott HT(ASCP) > Histology Supervisor > LBJ Hospital > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Wed Apr 16 10:34:45 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Apr 16 10:34:51 2008 Subject: [Histonet] Disposal of formalin containers In-Reply-To: References: <5b6eb13e0804160801qa6f5428t9578bc9649eafc0d@mail.gmail.com> Message-ID: <5b6eb13e0804160834w45bdee1erdbfb9b45779bc865@mail.gmail.com> Okay, good point. :) On Wed, Apr 16, 2008 at 8:30 AM, Jackie M O'Connor < Jackie.O'Connor@abbott.com > wrote: > > I wouldn't think so - aldehydes may have leeched into the plastic. Would > you buy a baby bottle made from recycled formalin containers? > > > > *"Mark Tarango" * > Sent by: histonet-bounces@lists.utsouthwestern.edu > > 04/16/2008 10:01 AM > To > "Scott, Allison D" > cc > histonet@lists.utsouthwestern.edu Subject > Re: [Histonet] Disposal of formalin containers > > > > > I wondering... can they could be recycled? > > On Wed, Apr 16, 2008 at 7:52 AM, Scott, Allison D < > Allison_Scott@hchd.tmc.edu> wrote: > > > Hello to all in histoland. Our safety officer inquired If we had a > > procedure for the disposal of our formalin bottles. The only procedure > > that I had was for the waste formalin. We just throw the bottles in the > > trash for disposal. Does any one do anything special for this type of > > disposal. Your help in this matter will be greatly appreciated. > > > > Allison Scott HT(ASCP) > > Histology Supervisor > > LBJ Hospital > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From marktarango <@t> gmail.com Wed Apr 16 10:53:15 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Apr 16 10:53:21 2008 Subject: [Histonet] Disposal of formalin containers In-Reply-To: <5b6eb13e0804160834w45bdee1erdbfb9b45779bc865@mail.gmail.com> References: <5b6eb13e0804160801qa6f5428t9578bc9649eafc0d@mail.gmail.com> <5b6eb13e0804160834w45bdee1erdbfb9b45779bc865@mail.gmail.com> Message-ID: <5b6eb13e0804160853q2c0f6381g9175db74949cc348@mail.gmail.com> I just got a comment off the list that says they don't make food containers from recycled plastic. If that's the case, can't we recycle formalin containers? On Wed, Apr 16, 2008 at 8:34 AM, Mark Tarango wrote: > Okay, good point. :) > > > On Wed, Apr 16, 2008 at 8:30 AM, Jackie M O'Connor < > Jackie.O'Connor@abbott.com > wrote: > > > > > I wouldn't think so - aldehydes may have leeched into the plastic. > > Would you buy a baby bottle made from recycled formalin containers? > > > > > > > > *"Mark Tarango" * > > Sent by: histonet-bounces@lists.utsouthwestern.edu > > > > 04/16/2008 10:01 AM > > To > > "Scott, Allison D" > > cc > > histonet@lists.utsouthwestern.edu Subject > > Re: [Histonet] Disposal of formalin containers > > > > > > > > > > I wondering... can they could be recycled? > > > > On Wed, Apr 16, 2008 at 7:52 AM, Scott, Allison D < > > Allison_Scott@hchd.tmc.edu> wrote: > > > > > Hello to all in histoland. Our safety officer inquired If we had a > > > procedure for the disposal of our formalin bottles. The only > > procedure > > > that I had was for the waste formalin. We just throw the bottles in > > the > > > trash for disposal. Does any one do anything special for this type of > > > disposal. Your help in this matter will be greatly appreciated. > > > > > > Allison Scott HT(ASCP) > > > Histology Supervisor > > > LBJ Hospital > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > From dbarkmei <@t> med.wayne.edu Wed Apr 16 11:17:31 2008 From: dbarkmei <@t> med.wayne.edu (Dan Barkmeier) Date: Wed Apr 16 11:17:36 2008 Subject: [Histonet] Frozen rat brains sections: OCT adheres, tissue does not In-Reply-To: References: <8a577a7f0804151108h7cd92bf7k8a1f7acd9f26a7d4@mail.gmail.com> Message-ID: <8a577a7f0804160917y20724a86se8128b7723c9ed11@mail.gmail.com> Thanks for the replies I've gotten thus far! I do find that warmer temperatures make it less likely that the tissue section will detach from the surrounding OCT, so that makes that problem less troublesome. What remains my main frustration now is the fact that *the rat brain sections show no particular attraction to the slide*. The OCT adheres wonderfully - both quickly and smoothly, but the tissue itself is very slow to attach and hardly seems attracted to the slide at all, even when I'm angling the slide downward. Does anyone have experience with a similar issue? I'm wondering if more or less fixation might have something to do with this? In response to some questions asked: - Yes, I do let the block equilibrate in the cryostat for about an hour before sectioning. I've even tried putting it in the -20 freezer overnight to make sure it's really up to temperature - No I have not tried snap-freezing fresh tissue. Would better results be expected from this? Would there be any problems using these sections for riboprobe in situ hybridizations or immunostaining for phosphorylated proteins? - My cryostat does not have separate object/rollbar temperature settings. Per some advice I found in the archive, I placed aluminum foil containers with dry ice near the blade and the anti-roll plate to keep them colder. This allowed me to cut at a warmer temperature than I previously had, but only up to about -14C, where the section scrunches up anyway. I have tried getting out a new box of slides from a newly-delivered batch to see if that made a difference, but it did not. I've also borrowed some hand-subbed slides from another lab to see if that helped the tissue attraction, but it didn't seem to make a difference. This problem has been variable - some brains work well, others seem impossible. Any insight into what variables to watch out for would be appreciated! - Dan On Tue, Apr 15, 2008 at 7:35 PM, Katie Glattfelder < KatieG@alleninstitute.org> wrote: > I cut fresh frozen mouse brains at 25 microns for about two years and we > frequently encountered the same issue. There are a couple of things you > can try: > > 1) try warming up your temperature a little bit - we usually found that > an object temp of -11 to -13 and a chamber temp of -14 to -16 worked > best > 2) try cooling your rollplate immediately before taking a section, > sometimes this can reduce the separation but often has to be done > frequently > 3) if you haven't already, try trimming your OCT fairly close (1-2 mm?) > at the edge that touches the blade first and try to catch the first part > of the section at the same time or close to the same time as the OCT, > then tilt the slide down quickly to get the whole section without > wrinkles or folds (it's hard to describe a technique with words!) > 4) try using Neg-50 instead of OCT. Neg-50 greatly reduced tissue > separating from the embedding media but takes some getting used to > because it "behaves" differently than OCT. If you use NEG 50, you > should reduce your temperatures by 2-3 degrees from what I recommended > above, and probably have some practice tissue to get used to it. > > Hope this helps! > > Katie > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dan > Barkmeier > Sent: Tuesday, April 15, 2008 11:09 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Frozen rat brains sections: OCT adheres, tissue does > not > > I've experienced ongoing frustrations with cryosectioning rat brains for > over a year now, and felt it was time to seek professional help. I'm > having > trouble getting good quality sections, which seems to stem from a > varying > mix of these two related problems: > > 1) Upon cutting, parts of the tissue section detach from the surrounding > OCT > embedding medium > > 2) When adhering the section to a slide, the OCT sticks immediately, but > the > section is not very adherent. The causes the perimeter of OCT to 'run' > around the section well before the neighboring tissue is stuck, and I > get > giant wrinkles in the section. > > Tissue processing: > - Quickly dissect brains, cut them into 2-5mm coronal slices, and place > them in 4% paraformaldehyde in PBS for 48 hours > - Cryoprotect in 30% sucrose in PBS until tissue sinks (1-2 days) > - Embed in OCT on dry ice > - Cut sections at 20um (I've tried 10um as well, with similar results), > and > at various temperatures from -17C to -22C, and mount on SuperFrost Plus > slides > > I don't really have problems cutting human brain samples prepared in the > same manner; it's just my rats that give me trouble. > > I would greatly appreciate any advice offered. I've searched through > the > archives as best I can, but I did not find these problems specifically > addressed. > > - Dan Barkmeier > Wayne State University School of Medicine > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jqb7 <@t> cdc.gov Wed Apr 16 11:28:07 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed Apr 16 11:28:22 2008 Subject: [Histonet] certification In-Reply-To: <0E394B648E5284478A6CCB78E5AFDA2705635627@hpes1.HealthPartners.int> References: <0E394B648E5284478A6CCB78E5AFDA2705635627@hpes1.HealthPartners.int> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A747A1F5@LTA3VS011.ees.hhs.gov> Wouldn't her transcripts reflect the name of the school as it was when she attended? If so, and it was accredited at that time, then I would think ASCP would accept them. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Wednesday, April 16, 2008 11:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] certification I have a histotech who has been in the field for 9 years, went to school for histology in 1999, (2 year Associates), passed the practical portion of the registry, but did not take the written within the 5 year allowance. She recently sent her $175 in and all paperwork to register for taking the test and has been told by ASCP that she is no longer eligible to take the test due to her credits not being from an accredited school. The school she attended was accredited and then taken over a few years ago by a University out of Chicago, so is now under new management and name. The present college can only accept "X" number of credits for transfer, even though it is the same program, same person in charge of the program ,etc. And, to top it all off, ASCP will not refund her entry fee nor hold it for her when she can take the exam. I find this hard to understand when there are so few schools around and techs are hard to find. This person is a very good tech and did not take the registry exam until we forced her hand, due to her being a single mom of twins with no financial support, etc. Has anyone ever heard of such a scenario and any ideas to help this tech, other than her trying to retake several college credits? Thanks for any ideas or suggestions to help out a fellow histotech!!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charles.Embrey <@t> carle.com Wed Apr 16 11:38:44 2008 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Wed Apr 16 11:38:49 2008 Subject: [Histonet] certification In-Reply-To: <0E394B648E5284478A6CCB78E5AFDA2705635627@hpes1.HealthPartners.int> References: <0E394B648E5284478A6CCB78E5AFDA2705635627@hpes1.HealthPartners.int> Message-ID: <44780C571F28624DBB446DE55C4D733A1FE5A8@EXCHANGEBE1.carle.com> I think the problem is that if you don't complete the process within the time period allowed then that route is closed to you and you must apply under a different route. Have her apply under the OJT route and take the exam that way. The credits for the OJT route don't have to be from a NAAClS accredited school. Simple transcripts from the old school should do the trick. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Wednesday, April 16, 2008 10:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] certification I have a histotech who has been in the field for 9 years, went to school for histology in 1999, (2 year Associates), passed the practical portion of the registry, but did not take the written within the 5 year allowance. She recently sent her $175 in and all paperwork to register for taking the test and has been told by ASCP that she is no longer eligible to take the test due to her credits not being from an accredited school. The school she attended was accredited and then taken over a few years ago by a University out of Chicago, so is now under new management and name. The present college can only accept "X" number of credits for transfer, even though it is the same program, same person in charge of the program ,etc. And, to top it all off, ASCP will not refund her entry fee nor hold it for her when she can take the exam. I find this hard to understand when there are so few schools around and techs are hard to find. This person is a very good tech and did not take the registry exam until we forced her hand, due to her being a single mom of twins with no financial support, etc. Has anyone ever heard of such a scenario and any ideas to help this tech, other than her trying to retake several college credits? Thanks for any ideas or suggestions to help out a fellow histotech!!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jason.Burrill <@t> crl.com Wed Apr 16 11:59:21 2008 From: Jason.Burrill <@t> crl.com (Burrill, Jason) Date: Wed Apr 16 11:59:45 2008 Subject: [Histonet] RE: Disposal of formalin containers In-Reply-To: References: Message-ID: <1AD4E907E9B6F648AEF1B3A20A9B0E1EEEE718@shr-exch2.na01.crl.com> Hi Allison, In our lab we dispose of the used 10% neutral buffered formalin in our designated formalin waste container then place a small amount of water in the container for a rinse then dispose of the rinse in the formalin waste container (that significantly reduces the likelihood of formaldehyde showing up in our waste water when it is tested). The formalin containers are subsequently rinsed and used again. This may not work for all labs or containers but it works for us. Jason Jason Burrill Sr. Manager, Histology and Laboratory Safety Charles River Laboratories 251 Ballardvale Street Wilmington, MA 01887 Ph: 781-222-6152 Fax: 978-988-8793 jason.burrill@crl.com **Please note new direct dial telephone number** ------------------------------ Message: 24 Date: Wed, 16 Apr 2008 09:52:34 -0500 From: "Scott, Allison D" Subject: [Histonet] Disposal of formalin containers To: histonet@lists.utsouthwestern.edu Message-ID: <1872B4A455B7974391609AD8034C79FC8BD41B@LBEXCH01.hchd.local> Content-Type: text/plain; charset="us-ascii" Hello to all in histoland. Our safety officer inquired If we had a procedure for the disposal of our formalin bottles. The only procedure that I had was for the waste formalin. We just throw the bottles in the trash for disposal. Does any one do anything special for this type of disposal. Your help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital ------------------------------ From Norm.Burnham <@t> propathlab.com Wed Apr 16 13:50:43 2008 From: Norm.Burnham <@t> propathlab.com (Norm Burnham) Date: Wed Apr 16 13:50:51 2008 Subject: [Histonet] Snap Freezing of Lymph Nodes for RNA testing Message-ID: Hi Histonet, Does anyone have a simple, inexpensive and very portable method for snap freezing lymph nodes in preparation for shipment on dry ice? We have considered Liquid Nitrogen and Isopentane bath, but I am looking for a fully portable method that can be handled in a doctors office by their staff. Thanks, Norm Burnham ____________________________________ Norm Burnham Director of Laboratory Operations ProPath - The Leader in Pathology Services(r) 8267 Elmbrook Drive, Suite 100 Dallas, TX 75247 214.237.1602 Office 214.237.1802 Fax 214.709.7127 Cell Email: norm.burnham@propath.com To learn more about ProPath, please visit www.ProPath.com ________________________________ ______________________________________________________________________________ This e-mail may contain confidential or privileged information. If you think you have received this e-mail in error, please advise the sender by reply e-mail and then delete this e-mail immediately. From c.m.vanderloos <@t> amc.uva.nl Wed Apr 16 13:52:17 2008 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Wed Apr 16 13:52:30 2008 Subject: [Histonet] RE: Ki-67 and Caspase 3 Message-ID: James,Go for rabbit monoclonal clone SP6 (Thermo, Vector) for Ki67 antibody that and rabbit anti-caspase-3 antibody from Cell Signalling. Both antibodies works on many different species.Your cryostat tissue section from fresh frozen blocks should be well dried (overnight, RT, under a fan) and fixed in NBF for 5 min at RT. Acetone-fixation makes your positive nuclei very blurry and diffuse. Wash 3-4x with TBS (or PBS) and start your immuno.Cheers, ChrisChris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The NetherlandsDate: Wed, 16 Apr 2008 07:15:19 -0700 (PDT) From: James Dooley Subject: [Histonet] Ki-67 and Caspase 3 To: histonet@lists.utsouthwestern.edu I would like to measure both proliferation and apoptosis. Can anyone recommend a good Ki-67 antibody of use on fresh frozen tissue to measure proliferation and an antibody for activated caspase 3 for apoptosis on mouse tissue. Please not fixation used if possible. I apologize for the confusion with my previous e-mail. Thank you, James From sheila_adey <@t> hotmail.com Wed Apr 16 15:16:36 2008 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Wed Apr 16 15:16:40 2008 Subject: [Histonet] Fixing & Processing Cell Pellets In-Reply-To: <4ECD18F12E443644B1F3C924A2039824839050@ikoexchange.ikonisys.com> References: <35e16a770804160744g78c62844g59db1dc1574afe44@mail.gmail.com> <4ECD18F12E443644B1F3C924A2039824839050@ikoexchange.ikonisys.com> Message-ID: We purchase a cyto block kit from Thermo Shandon. Works great.Sheila Adey HT MLTPort Huron HospitalMichigan> Date: Wed, 16 Apr 2008 11:02:52 -0400> From: angela.mcnabola@ikonisys.com> To: igor.deyneko@gmail.com; Histonet@lists.utsouthwestern.edu> Subject: RE: [Histonet] Fixing & Processing Cell Pellets> CC: > > We used to use Histo-gel from Richard Allen, it worked quite nicely,> kept the pellet together and processed well. It didn't interfere with> any IHC either.> > I'm sorry that I can not give you more specifics as I am currently in a> new position without our protocol in from of me and do not want to give> you any misinformation, but I believe that after the fixation, we> followed the manufacturer's protocol. Here is the link:> http://www.rallansci.com/histology/histology.aspx?id=2> > > > Angela McNabola, MS HT(ASCP)SLS, QIHC> Research Scientist> Ikonisys, Inc.> 5 Science Park> New Haven, CT 06511> 203-776-0791> angela.mcnabola@ikonisys.com> > -----Original Message-----> From: histonet-bounces@lists.utsouthwestern.edu> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Igor> Deyneko> Sent: Wednesday, April 16, 2008 10:45 AM> To: Histonet@lists.utsouthwestern.edu> Subject: [Histonet] Fixing & Processing Cell Pellets> > Dear Histonetters!> I was wondering if anyone knows or has a good protocol for processing,> fixing, and embedding the pelleted cells. I was given a protocol by my> boss,> but I have a few questions about it.> Protocol I got:> > Pellet cells, wash with PBS, re-pellet.> > Remove PBS and gently add 10%NBF.> > 10NBF-until fixed 6-24 hours.> > 70-85-95-95-100-100-100% Ethanols(15-20 minutes each).> > 3 xylene changes 20 minutes each.> > 4 paraffin changes 30 minutes each(melted paraffin is the first heated> step> 60 degrees).> > Embed and cut at 5 microns using a rotary microtome and accu edge> blades,> lay out on a 47 degree water bath, let dry on a flat warming table or in> the> oven and they are ready for deparaffinization.> > So I have questions as to how would i extract cellf from the test tube> without disturbing the pellet? Then what could I put them into for> dehydration with alcohols and clearance with xylene? As far as paraffin> goes> as well,are there any special cassettes designed for cells? Also as far> as> embedding goes, would I do the standard embedding or are there special> techniques? I would appreciate any information/ protocols.> > Thank you in advance.> > Igor Deyneko> > Infinity Pharmaceuticals,> > Cambridge, MA> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Try Chicktionary, a game that tests how many words you can form from the letters given. Find this and more puzzles at Live Search Games! http://g.msn.ca/ca55/207 From LINDA.MARGRAF <@t> childrens.com Wed Apr 16 15:47:57 2008 From: LINDA.MARGRAF <@t> childrens.com (LINDA MARGRAF) Date: Wed Apr 16 15:48:18 2008 Subject: [Histonet] Glut-1 Message-ID: <48061FAD020000DA00024E82@CNET3.CHILDRENS.COM> Histonetters: I understand Dako is discontinuing its Glut-1 antibody. Does anyone use a different vendor's antibody they'd recommend for formalin fixed paraffin embedded human tissue specimens (routine diagnostic cases of vascular lesions)? Thanks Linda M Histonet administrator From MLunetta <@t> luhcares.org Wed Apr 16 16:04:45 2008 From: MLunetta <@t> luhcares.org (Matthew Lunetta) Date: Wed Apr 16 16:05:16 2008 Subject: [Histonet] Lycing of RBC on Bone Marrow Aspirate Slides Message-ID: <4806158D020000A80001B689@mail.luhcares.org> Hello Histonetters, I am looking at doing some research into the lycing of Red Blood Cells after the Bone Marrow aspirate is already collected onto the slide. I was wondering if there has already been some work in this area. If anyone has any info please let me know. thanks Matt Lunetta Longmont United Hospital Colorado From laurie.colbert <@t> huntingtonhospital.com Wed Apr 16 16:21:12 2008 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Wed Apr 16 16:21:17 2008 Subject: [Histonet] Histobath, freezing fresh tissue Message-ID: <57BE698966D5C54EAE8612E8941D768302C13FB6@EXCHANGE3.huntingtonhospital.com> I am looking for a replacement for our Histobath, which just died. Thermo Fisher no longer carries this item and has no substitute. The Histobath is a small piece of electrical equipment that utilizes methylbutane to quickly freeze fresh tissue. We used to use liquid nitrogen but found this was much easier. Can anyone tell me where I can find a similar piece of equipment, or what your method is for freezing tissue. Thanks, Laurie Colbert Huntington Hospital From mpence <@t> grhs.net Wed Apr 16 16:26:49 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Apr 16 16:26:55 2008 Subject: [Histonet] Lycing of RBC on Bone Marrow Aspirate Slides In-Reply-To: <4806158D020000A80001B689@mail.luhcares.org> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A37DF@IS-E2K3.grhs.net> Try 3% H2O2 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Lunetta Sent: Wednesday, April 16, 2008 4:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Lycing of RBC on Bone Marrow Aspirate Slides Hello Histonetters, I am looking at doing some research into the lycing of Red Blood Cells after the Bone Marrow aspirate is already collected onto the slide. I was wondering if there has already been some work in this area. If anyone has any info please let me know. thanks Matt Lunetta Longmont United Hospital Colorado _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gmartin <@t> marshallmedical.org Wed Apr 16 16:28:45 2008 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Wed Apr 16 16:28:55 2008 Subject: [Histonet] Slide labeling Message-ID: <6ED9D4252F278841A0593D3D788AF24C0233CDB7@mailsvr.MARSHMED.local> We are in the process of changing how we label slides and blocks ... here is the question at hand; Should a single specimen, meaning on block one slide, be labeled S08-01500-A or should it be labeled S08-01500. Thanks in advance. Gary From AnthonyH <@t> chw.edu.au Wed Apr 16 16:46:56 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Apr 16 16:48:11 2008 Subject: [Histonet] Lycing of RBC on Bone Marrow Aspirate Slides In-Reply-To: <4806158D020000A80001B689@mail.luhcares.org> Message-ID: If you want an easy way to lyse rbc's from a slide the following method works quite well for cytologists who wish to have a 95% ethanol fixed-like appearance: Ng et al Re-hydration Method for Red Blood Cell Lysis Principle: Rehydration of air dried smears with normal saline for 30 seconds before fixation in alcohol has been found to produce slides of a quality superior or at least equivalent to that of immediate fixed smears. It allowed the cells to adhere better to the slide and lysis of red blood cells provided a clean background for better assessment. Solutions: 1. Normal Saline Sodium Chloride 9g Distilled water 1000ml 2. 95% Ethanol Procedure: 1. After smearing material, air dry slides using a hair dryer. 2. Place slides in Normal Saline for 30sec. 3. Immediately place slides in 95% ethanol and fix for 10 minutes. 4. Stain slides via PAP. Results: RBC were effectively lysed but epithelial and mesothelial cells were retained. In general, smears showed a decrease in chromaticity of both nuclear and cytoplasmic staining. Cytoplasmic vacuoles were less distinct and epithelial fragments, such as acini, appeared flattened and could be more easily focussed on the same plane. Nuclear and cellular moulding was exaggerated and more appreciable when present. There was a slight enlargement of cells. Degenerated specimens, even in the absence of polymorphs, often resulted in disappointing re-hydrated smears. Cell clusters were easier to see probably because of the higher transparency of the cytoplasm. Reference: Acta Cytolog (1994) 38(1):56-64. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Lunetta Sent: Thursday, 17 April 2008 7:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Lycing of RBC on Bone Marrow Aspirate Slides Hello Histonetters, I am looking at doing some research into the lycing of Red Blood Cells after the Bone Marrow aspirate is already collected onto the slide. I was wondering if there has already been some work in this area. If anyone has any info please let me know. thanks Matt Lunetta Longmont United Hospital Colorado _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From victor <@t> pathology.washington.edu Wed Apr 16 17:07:40 2008 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Wed Apr 16 17:07:45 2008 Subject: [Histonet] Slide labeling In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C0233CDB7@mailsvr.MARSHMED.local> References: <6ED9D4252F278841A0593D3D788AF24C0233CDB7@mailsvr.MARSHMED.local> Message-ID: <480678AC.4090400@pathology.washington.edu> I would recommend labeling with A because what if you get a later unexpected specimen. Our LIS automatically assigns the first specimen as A. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Martin, Gary wrote: > We are in the process of changing how we label slides and blocks ... > here is the question at hand; > > Should a single specimen, meaning on block one slide, be labeled > S08-01500-A or should it be labeled S08-01500. > > Thanks in advance. > > Gary > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jnocito <@t> satx.rr.com Wed Apr 16 18:20:09 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Apr 16 18:20:13 2008 Subject: [Histonet] Disposal of formalin containers References: <1872B4A455B7974391609AD8034C79FC8BD41B@LBEXCH01.hchd.local> Message-ID: <004201c8a018$69fac300$0302a8c0@yourxhtr8hvc4p> Allison, at my former lab, we put the empty containers in a biohazard box to be picked up and burned. My current lab just throws them in the regular trash. I'm sure that helps a lot, doesn't it. the best answer would be get with your local landfill and see what they say. JTT ----- Original Message ----- From: "Scott, Allison D" To: Sent: Wednesday, April 16, 2008 9:52 AM Subject: [Histonet] Disposal of formalin containers Hello to all in histoland. Our safety officer inquired If we had a procedure for the disposal of our formalin bottles. The only procedure that I had was for the waste formalin. We just throw the bottles in the trash for disposal. Does any one do anything special for this type of disposal. Your help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Wed Apr 16 19:20:36 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Wed Apr 16 19:20:48 2008 Subject: [Histonet] Snap Freezing of Lymph Nodes for RNA testing References: Message-ID: <003d01c8a020$dc1fac40$6501a8c0@DHXTS541> It may be better to use a transportable Dewar flask for just the liquid nitrogen. I don't think a doctors office will want to handle isopentane, too volatile, flammable and explosive. Contact or maybe those on Histonet in dermatopathology offices have ways to do this for biopsies they collect, freeze and ship. Good luck Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Norm Burnham" To: Sent: Wednesday, April 16, 2008 12:50 PM Subject: [Histonet] Snap Freezing of Lymph Nodes for RNA testing Hi Histonet, Does anyone have a simple, inexpensive and very portable method for snap freezing lymph nodes in preparation for shipment on dry ice? We have considered Liquid Nitrogen and Isopentane bath, but I am looking for a fully portable method that can be handled in a doctors office by their staff. Thanks, Norm Burnham ____________________________________ Norm Burnham Director of Laboratory Operations ProPath - The Leader in Pathology Services(r) 8267 Elmbrook Drive, Suite 100 Dallas, TX 75247 214.237.1602 Office 214.237.1802 Fax 214.709.7127 Cell Email: norm.burnham@propath.com To learn more about ProPath, please visit www.ProPath.com ________________________________ ______________________________________________________________________________ This e-mail may contain confidential or privileged information. If you think you have received this e-mail in error, please advise the sender by reply e-mail and then delete this e-mail immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Norm.Burnham <@t> propathlab.com Wed Apr 16 20:31:01 2008 From: Norm.Burnham <@t> propathlab.com (Norm Burnham) Date: Wed Apr 16 20:30:33 2008 Subject: [Histonet] Snap Freezing of Lymph Nodes for RNA testing Message-ID: Hi Gayle, Thank you for your advice. We want to avoid LN and Isopentane if at all possible. We are looking for a simple, inexpensive, easy to use, and RNAse free method to preserve, stabilize and transport tissue for RNA studies. Does anyone have an alternative to LN or Isopentane bath for snap freezing followed by shipment on dry ice, or an alternate way, to stabilize tissue for periods ranging from a few hours to perhaps as long as 72 hours? Thanks and best regards, Norm Burnham ----- Original Message ----- From: Gayle Callis To: Norm Burnham; histonet@lists.utsouthwestern.edu Sent: Wed Apr 16 19:20:36 2008 Subject: Re: [Histonet] Snap Freezing of Lymph Nodes for RNA testing It may be better to use a transportable Dewar flask for just the liquid nitrogen. I don't think a doctors office will want to handle isopentane, too volatile, flammable and explosive. Contact or maybe those on Histonet in dermatopathology offices have ways to do this for biopsies they collect, freeze and ship. Good luck Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Norm Burnham" To: Sent: Wednesday, April 16, 2008 12:50 PM Subject: [Histonet] Snap Freezing of Lymph Nodes for RNA testing Hi Histonet, Does anyone have a simple, inexpensive and very portable method for snap freezing lymph nodes in preparation for shipment on dry ice? We have considered Liquid Nitrogen and Isopentane bath, but I am looking for a fully portable method that can be handled in a doctors office by their staff. Thanks, Norm Burnham ____________________________________ Norm Burnham Director of Laboratory Operations ProPath - The Leader in Pathology Services(r) 8267 Elmbrook Drive, Suite 100 Dallas, TX 75247 214.237.1602 Office 214.237.1802 Fax 214.709.7127 Cell Email: norm.burnham@propath.com To learn more about ProPath, please visit www.ProPath.com ________________________________ _____________________________________________________________________________ _ This e-mail may contain confidential or privileged information. If you think you have received this e-mail in error, please advise the sender by reply e-mail and then delete this e-mail immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________________ This e-mail may contain confidential or privileged information. If you think you have received this e-mail in error, please advise the sender by reply e-mail and then delete this e-mail immediately. From anh2006 <@t> med.cornell.edu Wed Apr 16 21:22:25 2008 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Wed Apr 16 21:22:35 2008 Subject: [Histonet] Snap Freezing of Lymph Nodes for RNA testing Message-ID: <3bef06073ffe.48067c21@med.cornell.edu> If you are extracting RNA from the nodes eventually why not use a solution called "RNA Later". You can then ship them without dry ice. Works very well in my experience. It's from ambion.com http://www.ambion.com/catalog/CatNum.php?AM7020 Otherwise I agree with Gayle, don't use the isopentane. You could just put the tissue in a flexible tube or in a foil and drop it into the liquid nitrogen in a portable Dewar. It's not ideal but it will work fine. ----- Original Message ----- From: Gayle Callis Date: Wednesday, April 16, 2008 8:20 pm Subject: Re: [Histonet] Snap Freezing of Lymph Nodes for RNA testing > It may be better to use a transportable Dewar flask for just the > liquid > nitrogen. I don't think a doctors office will want to handle > isopentane, > too volatile, flammable and explosive. Contact or maybe those on > Histonet > in dermatopathology offices have ways to do this for biopsies they > collect, > freeze and ship. > > Good luck > > Gayle M. Callis > HTL/HT/MT(ASCP) > Bozeman MT 59715 > > > ----- Original Message ----- > From: "Norm Burnham" > To: > Sent: Wednesday, April 16, 2008 12:50 PM > Subject: [Histonet] Snap Freezing of Lymph Nodes for RNA testing > > > Hi Histonet, > > > > Does anyone have a simple, inexpensive and very portable method for > snapfreezing lymph nodes in preparation for shipment on dry ice? > We have > considered Liquid Nitrogen and Isopentane bath, but I am looking > for a fully > portable method that can be handled in a doctors office by their > staff. > > > Thanks, > > > > Norm Burnham > > > > ____________________________________ > > Norm Burnham > Director of Laboratory Operations > ProPath - The Leader in Pathology Services(r) > > 8267 Elmbrook Drive, Suite 100 > Dallas, TX 75247 > 214.237.1602 Office > 214.237.1802 Fax > > 214.709.7127 Cell > > Email: norm.burnham@propath.com > > > To learn more about ProPath, please visit www.ProPath.com > > > ________________________________ > > > > ______________________________________________________________________________ > > > This e-mail may contain confidential or privileged information. If > you think > you have received this e-mail > in error, please advise the sender by reply e-mail and then delete > this > e-mail immediately. > > > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From anh2006 <@t> med.cornell.edu Wed Apr 16 21:24:56 2008 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Wed Apr 16 21:25:02 2008 Subject: [Histonet] Snap Freezing of Lymph Nodes for RNA testing Message-ID: <326fa1d51c0d.48067cb8@med.cornell.edu> Ooops, read this after I already wrote my message 2nding the liquid nitrogen suggestion. Well then, based on your comments below I think the RNA Later is ideal. Otherwise you could put the tissue (in small pieces or chopped up) directly into Trizol and ship that way. I have received tissues as such as they have been fine for RNA. But truthfully, RNA Later is probably the safest, easiest way to go. ----- Original Message ----- From: Norm Burnham Date: Wednesday, April 16, 2008 9:31 pm Subject: Re: [Histonet] Snap Freezing of Lymph Nodes for RNA testing > Hi Gayle, > Thank you for your advice. We want to avoid LN and Isopentane if at > allpossible. We are looking for a simple, inexpensive, easy to > use, and RNAse > free method to preserve, stabilize and transport tissue for RNA > studies.Does anyone have an alternative to LN or Isopentane bath > for snap freezing > followed by shipment on dry ice, or an alternate way, to stabilize > tissue for > periods ranging from a few hours to perhaps as long as 72 hours? > Thanks and best regards, > Norm Burnham > > ----- Original Message ----- > From: Gayle Callis > To: Norm Burnham; histonet@lists.utsouthwestern.edu > > Sent: Wed Apr 16 19:20:36 2008 > Subject: Re: [Histonet] Snap Freezing of Lymph Nodes for RNA testing > > It may be better to use a transportable Dewar flask for just the > liquid > nitrogen. I don't think a doctors office will want to handle > isopentane, > too volatile, flammable and explosive. Contact or maybe those on > Histonet > in dermatopathology offices have ways to do this for biopsies they > collect, > freeze and ship. > > Good luck > > Gayle M. Callis > HTL/HT/MT(ASCP) > Bozeman MT 59715 > > > ----- Original Message ----- > From: "Norm Burnham" > To: > Sent: Wednesday, April 16, 2008 12:50 PM > Subject: [Histonet] Snap Freezing of Lymph Nodes for RNA testing > > > Hi Histonet, > > > > Does anyone have a simple, inexpensive and very portable method for > snapfreezing lymph nodes in preparation for shipment on dry ice? > We have > considered Liquid Nitrogen and Isopentane bath, but I am looking > for a fully > portable method that can be handled in a doctors office by their > staff. > > > Thanks, > > > > Norm Burnham > > > > ____________________________________ > > Norm Burnham > Director of Laboratory Operations > ProPath - The Leader in Pathology Services(r) > > 8267 Elmbrook Drive, Suite 100 > Dallas, TX 75247 > 214.237.1602 Office > 214.237.1802 Fax > > 214.709.7127 Cell > > Email: norm.burnham@propath.com > > > To learn more about ProPath, please visit www.ProPath.com > > > ________________________________ > > > > _____________________________________________________________________________ > _ > > > This e-mail may contain confidential or privileged information. If > you think > you have received this e-mail > in error, please advise the sender by reply e-mail and then delete > this > e-mail immediately. > > > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ______________________________________________________________________________ > > > This e-mail may contain confidential or privileged information. If > you think you have received this e-mail > in error, please advise the sender by reply e-mail and then delete > this e-mail immediately. > > > > > > > > > > -------------- next part -------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BMolinari <@t> heart.thi.tmc.edu Thu Apr 17 07:05:56 2008 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Thu Apr 17 07:06:04 2008 Subject: [Histonet] RE: Disposal of formalin containers In-Reply-To: <1AD4E907E9B6F648AEF1B3A20A9B0E1EEEE718@shr-exch2.na01.crl.com> References: <1AD4E907E9B6F648AEF1B3A20A9B0E1EEEE718@shr-exch2.na01.crl.com> Message-ID: We use the 5 gal carboys. We have the hospital waste dept pick up the empty containers. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC 1-283 Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Burrill, Jason Sent: Wednesday, April 16, 2008 11:59 AM To: histonet@lists.utsouthwestern.edu; Scott, Allison D Subject: [Histonet] RE: Disposal of formalin containers Hi Allison, In our lab we dispose of the used 10% neutral buffered formalin in our designated formalin waste container then place a small amount of water in the container for a rinse then dispose of the rinse in the formalin waste container (that significantly reduces the likelihood of formaldehyde showing up in our waste water when it is tested). The formalin containers are subsequently rinsed and used again. This may not work for all labs or containers but it works for us. Jason Jason Burrill Sr. Manager, Histology and Laboratory Safety Charles River Laboratories 251 Ballardvale Street Wilmington, MA 01887 Ph: 781-222-6152 Fax: 978-988-8793 jason.burrill@crl.com **Please note new direct dial telephone number** ------------------------------ Message: 24 Date: Wed, 16 Apr 2008 09:52:34 -0500 From: "Scott, Allison D" Subject: [Histonet] Disposal of formalin containers To: histonet@lists.utsouthwestern.edu Message-ID: <1872B4A455B7974391609AD8034C79FC8BD41B@LBEXCH01.hchd.local> Content-Type: text/plain; charset="us-ascii" Hello to all in histoland. Our safety officer inquired If we had a procedure for the disposal of our formalin bottles. The only procedure that I had was for the waste formalin. We just throw the bottles in the trash for disposal. Does any one do anything special for this type of disposal. Your help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Thu Apr 17 07:30:23 2008 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Thu Apr 17 07:32:44 2008 Subject: [Histonet] Slide labeling References: <6ED9D4252F278841A0593D3D788AF24C0233CDB7@mailsvr.MARSHMED.local> Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F25E9@fhosxchmb006.ADVENTISTCORP.NET> I always label it with the "A" since you may not be sure more tissue on the specimen will be submitted. With a small biopsy it probably doesn't matter, but I label it with the "A" for consistency. Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Martin, Gary Sent: Wed 4/16/2008 5:28 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide labeling We are in the process of changing how we label slides and blocks ... here is the question at hand; Should a single specimen, meaning on block one slide, be labeled S08-01500-A or should it be labeled S08-01500. Thanks in advance. Gary _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From lab.mef <@t> smmc.org Thu Apr 17 07:34:31 2008 From: lab.mef <@t> smmc.org (Meredith Fuller-Fedorczyk) Date: Thu Apr 17 07:34:52 2008 Subject: [Histonet] Snap Freezing of Lymph Nodes for RNA testing References: Message-ID: <006001c8a087$62759000$240c0180@PCLAB11> For snap freezing muscle biopsies to send on dry ice we use a 100% Alcohol or Acetone mixed with dry ice slurry. 1. Wrap dry ice pellets in a towel, pulverize the dry ice with a hammer than pour the powder dry ice into a 200 ml beaker. 2. Slowly add 100% alcohol and stir the mixture. At least 80% of the total volume of the slurry should consist of dry ice and only 20% alcohol. ( Acetone can replace 100% alcohol in the slurry) 3.When the slurry stops bubbling, the temperature has fallen to around -70C, it is then suitable for flash freezing. Keep adding dry ice as needed to reach this point. The final product should resemble a "snowcone". 4. Hold the biopsy specimen with forceps, and plunge tissue into the slurry quickly. Swirl the specimen in the slurry for 10-15 seconds, remove and quickly blot dry with an absorbent towel to remove excess alcohol. Immediately place tissue in a pre frozen specimen container that has prechilled on dry ice. A well frozen specimen should have a white chalky color. 5. Place cover on container. (puncture lid prior to allow excess alcohol to evaporate.) 6. Ship away on dry ice or store in a freezer that goes down to -70 C. We have a Jewitt freezer. Hope this helps you. It is a inexpensive alternative. Credit goes to Mayo Medical Services where this system is recommended for shipping to them. ----- Original Message ----- From: "Norm Burnham" To: ; Sent: Wednesday, April 16, 2008 9:31 PM Subject: Re: [Histonet] Snap Freezing of Lymph Nodes for RNA testing > Hi Gayle, > Thank you for your advice. We want to avoid LN and Isopentane if at all > possible. We are looking for a simple, inexpensive, easy to use, and RNAse > free method to preserve, stabilize and transport tissue for RNA studies. > Does anyone have an alternative to LN or Isopentane bath for snap freezing > followed by shipment on dry ice, or an alternate way, to stabilize tissue for > periods ranging from a few hours to perhaps as long as 72 hours? > Thanks and best regards, > Norm Burnham > > ----- Original Message ----- > From: Gayle Callis > To: Norm Burnham; histonet@lists.utsouthwestern.edu > > Sent: Wed Apr 16 19:20:36 2008 > Subject: Re: [Histonet] Snap Freezing of Lymph Nodes for RNA testing > > It may be better to use a transportable Dewar flask for just the liquid > nitrogen. I don't think a doctors office will want to handle isopentane, > too volatile, flammable and explosive. Contact or maybe those on Histonet > in dermatopathology offices have ways to do this for biopsies they collect, > freeze and ship. > > Good luck > > Gayle M. Callis > HTL/HT/MT(ASCP) > Bozeman MT 59715 > > > ----- Original Message ----- > From: "Norm Burnham" > To: > Sent: Wednesday, April 16, 2008 12:50 PM > Subject: [Histonet] Snap Freezing of Lymph Nodes for RNA testing > > > Hi Histonet, > > > > Does anyone have a simple, inexpensive and very portable method for snap > freezing lymph nodes in preparation for shipment on dry ice? We have > considered Liquid Nitrogen and Isopentane bath, but I am looking for a fully > portable method that can be handled in a doctors office by their staff. > > > > Thanks, > > > > Norm Burnham > > > > ____________________________________ > > Norm Burnham > Director of Laboratory Operations > ProPath - The Leader in Pathology Services(r) > > 8267 Elmbrook Drive, Suite 100 > Dallas, TX 75247 > 214.237.1602 Office > 214.237.1802 Fax > > 214.709.7127 Cell > > Email: norm.burnham@propath.com > > > To learn more about ProPath, please visit www.ProPath.com > > > ________________________________ > > > > ____________________________________________________________________________ _ > _ > > > This e-mail may contain confidential or privileged information. If you think > you have received this e-mail > in error, please advise the sender by reply e-mail and then delete this > e-mail immediately. > > > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ____________________________________________________________________________ __ > > > This e-mail may contain confidential or privileged information. If you think you have received this e-mail > in error, please advise the sender by reply e-mail and then delete this e-mail immediately. > > > > > > > > > > ---------------------------------------------------------------------------- ---- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From AWeiss <@t> shorememorial.org Thu Apr 17 07:39:11 2008 From: AWeiss <@t> shorememorial.org (AWeiss@shorememorial.org) Date: Thu Apr 17 07:39:21 2008 Subject: [Histonet] Re: Histonet Digest, Vol 53, Issue 24 In-Reply-To: <20080416152647.A7A0E4EC621@smhex1.shorememorial.org> Message-ID: Dear Histonetters! I was wondering if anyone knows or has a good protocol for processing, fixing, and embedding the pelleted cells. I was given a protocol by my boss, but I have a few questions about it. Protocol I got: Pellet cells, wash with PBS, re-pellet. Remove PBS and gently add 10%NBF. 10NBF-until fixed 6-24 hours. 70-85-95-95-100-100-100% Ethanols(15-20 minutes each). 3 xylene changes 20 minutes each. 4 paraffin changes 30 minutes each(melted paraffin is the first heated step 60 degrees). Embed and cut at 5 microns using a rotary microtome and accu edge blades, lay out on a 47 degree water bath, let dry on a flat warming table or in the oven and they are ready for deparaffinization. So I have questions as to how would i extract cellf from the test tube without disturbing the pellet? Then what could I put them into for dehydration with alcohols and clearance with xylene? As far as paraffin goes as well,are there any special cassettes designed for cells? Also as far as embedding goes, would I do the standard embedding or are there special techniques? I would appreciate any information/ protocols. Thank you in advance. Igor Deyneko Infinity Pharmaceuticals, Cambridge, MA You can suspend the cells in histogel by surgipath and this will create a envelope around the cells, you just need a small amt. to cover the cells. Allow it to soldify and then release and process you would any other tissue. Hope this helps. Andrea J Weiss BST CT (ASCP) Cytotechnologist 609 653 3577 Ext 4907 aweiss@shorememorial.org From pruegg <@t> ihctech.net Thu Apr 17 07:51:58 2008 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Thu Apr 17 07:44:34 2008 Subject: [Histonet] RE: Ki-67 and Caspase 3 In-Reply-To: Message-ID: <200804171244.m3HCiC5x084823@pro42.abac.com> James I to recommend the rab mono ki67 from Labvision and for CC3 although I have used the rab one from Cell Signaling and it works, I now get rab CC3 from BioCare it works as well in my hands and is much cheaper. As Chris says, I would use formalin fixation on frozens for these two markers. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of C.M. van der Loos Sent: Wednesday, April 16, 2008 12:52 PM To: histonet@lists.utsouthwestern.edu Cc: jdooley2008@yahoo.com Subject: [Histonet] RE: Ki-67 and Caspase 3 James,Go for rabbit monoclonal clone SP6 (Thermo, Vector) for Ki67 antibody that and rabbit anti-caspase-3 antibody from Cell Signalling. Both antibodies works on many different species.Your cryostat tissue section from fresh frozen blocks should be well dried (overnight, RT, under a fan) and fixed in NBF for 5 min at RT. Acetone-fixation makes your positive nuclei very blurry and diffuse. Wash 3-4x with TBS (or PBS) and start your immuno.Cheers, ChrisChris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The NetherlandsDate: Wed, 16 Apr 2008 07:15:19 -0700 (PDT) From: James Dooley Subject: [Histonet] Ki-67 and Caspase 3 To: histonet@lists.utsouthwestern.edu I would like to measure both proliferation and apoptosis. Can anyone recommend a good Ki-67 antibody of use on fresh frozen tissue to measure proliferation and an antibody for activated caspase 3 for apoptosis on mouse tissue. Please not fixation used if possible. I apologize for the confusion with my previous e-mail. Thank you, James _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From petepath <@t> yahoo.com Thu Apr 17 09:39:34 2008 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Thu Apr 17 09:39:38 2008 Subject: [Histonet] Histobath, freezing fresh tissue Message-ID: <347916.86790.qm@web45105.mail.sp1.yahoo.com> Dear Lori, If you are freezing your fresh tissues for intraoperative frozen section, you may want to try using our Precision Cryoembedding System which uses stainless steel well bars kept in the cryostat to freeze tissues face down in a precisely flat plane. I have converted many labs from freezing baths and liqid nitrogen. If avoiding freeze artefact is critical, the bars can be kept at very cold temps and filled with cold embedding medium to acheive minimal artefact. Please visit http://pathologyinnovations.com/index.html to learn more about the system. Stephen Peters M.D. Vice Chairman of Pathology Hackensack University Medical Center 201 996 4836 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com From Erin.Martin <@t> ucsf.edu Thu Apr 17 12:28:08 2008 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Thu Apr 17 12:29:40 2008 Subject: [Histonet] California techs Message-ID: Hi everyone, This came up a few days ago but I didn't see anything specific. Could anyone in CA tell me what the story is regarding the governor's push for licensure? Thanks Erin From cmiller <@t> physlab.com Thu Apr 17 12:38:17 2008 From: cmiller <@t> physlab.com (Cheri Miller) Date: Thu Apr 17 12:37:19 2008 Subject: [Histonet] certification In-Reply-To: <44780C571F28624DBB446DE55C4D733A1FE5A8@EXCHANGEBE1.carle.com> References: <0E394B648E5284478A6CCB78E5AFDA2705635627@hpes1.HealthPartners.int> <44780C571F28624DBB446DE55C4D733A1FE5A8@EXCHANGEBE1.carle.com> Message-ID: <000901c8a0b1$d25524b0$3d02a8c0@plab.local> I agree with you Charles, I currently have 2 OJT techs training and they have the proper amount of college credits( min 12 hours of the sciences) equaling to an AA or BA depending on the tech. They can sit for the test after one year working in a histology lab. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles.Embrey Sent: Wednesday, April 16, 2008 11:39 AM To: Webb, Dorothy L; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] certification I think the problem is that if you don't complete the process within the time period allowed then that route is closed to you and you must apply under a different route. Have her apply under the OJT route and take the exam that way. The credits for the OJT route don't have to be from a NAAClS accredited school. Simple transcripts from the old school should do the trick. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Wednesday, April 16, 2008 10:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] certification I have a histotech who has been in the field for 9 years, went to school for histology in 1999, (2 year Associates), passed the practical portion of the registry, but did not take the written within the 5 year allowance. She recently sent her $175 in and all paperwork to register for taking the test and has been told by ASCP that she is no longer eligible to take the test due to her credits not being from an accredited school. The school she attended was accredited and then taken over a few years ago by a University out of Chicago, so is now under new management and name. The present college can only accept "X" number of credits for transfer, even though it is the same program, same person in charge of the program ,etc. And, to top it all off, ASCP will not refund her entry fee nor hold it for her when she can take the exam. I find this hard to understand when there are so few schools around and techs are hard to find. This person is a very good tech and did not take the registry exam until we forced her hand, due to her being a single mom of twins with no financial support, etc. Has anyone ever heard of such a scenario and any ideas to help this tech, other than her trying to retake several college credits? Thanks for any ideas or suggestions to help out a fellow histotech!!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From AWempren <@t> skcc.org Thu Apr 17 12:52:32 2008 From: AWempren <@t> skcc.org (Alexina Wempren) Date: Thu Apr 17 12:52:38 2008 Subject: [Histonet] endothelial markers for dog vessels Message-ID: Hi, Can anyone tell me what antibodies they use for dog vessels? Thanks! alexina wempren From Dorothy.L.Webb <@t> HealthPartners.Com Thu Apr 17 12:54:30 2008 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Thu Apr 17 12:54:35 2008 Subject: [Histonet] labeling Message-ID: <0E394B648E5284478A6CCB78E5AFDA270563563A@hpes1.HealthPartners.int> We do not use the "A" designation unless there is more than one specimen. We found it to be less confusing to all involved and the "A" signals multiple specimens without having to look the case up! I don't think there is any "correct" way, just what your institution prefers!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From phiho.hoang <@t> rogers.com Thu Apr 17 12:56:09 2008 From: phiho.hoang <@t> rogers.com (PhiHo Hoang) Date: Thu Apr 17 12:56:13 2008 Subject: [Histonet] [Off Topic] Aseptic Thin Layer of Plant Cells Message-ID: <017701c8a0b4$51a42b10$f4ec8130$@hoang@rogers.com> Greetings, I need live plant cells in a thin slices of plant tissue (around 3 to 6 plant cells thick). These thin slices will be used as explants in plant tissue cultures. How can I use a microtome to achieve this? Your help is greatly appreciated. Cheers, PhiHo From shive003 <@t> umn.edu Thu Apr 17 12:56:22 2008 From: shive003 <@t> umn.edu (Jan Shivers) Date: Thu Apr 17 12:56:29 2008 Subject: [Histonet] endothelial markers for dog vessels References: Message-ID: <00ed01c8a0b4$59572560$a3065486@auxs.umn.edu> Factor VIII-Related Antigen, assuming you mean 'blood' vessels. Jan Shivers Senior Scientist Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu ----- Original Message ----- From: "Alexina Wempren" To: Sent: Thursday, April 17, 2008 12:52 PM Subject: [Histonet] endothelial markers for dog vessels Hi, Can anyone tell me what antibodies they use for dog vessels? Thanks! alexina wempren _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Thu Apr 17 13:02:20 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Thu Apr 17 13:03:18 2008 Subject: [Histonet] certification In-Reply-To: <000901c8a0b1$d25524b0$3d02a8c0@plab.local> References: <0E394B648E5284478A6CCB78E5AFDA2705635627@hpes1.HealthPartners.int> <44780C571F28624DBB446DE55C4D733A1FE5A8@EXCHANGEBE1.carle.com> <000901c8a0b1$d25524b0$3d02a8c0@plab.local> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A747A213@LTA3VS011.ees.hhs.gov> I thought part of the problem was she could not get all of her credits to transfer because the school has since changed hands and will not accept all of them. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Thursday, April 17, 2008 1:38 PM To: 'Charles.Embrey'; 'Webb, Dorothy L'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] certification I agree with you Charles, I currently have 2 OJT techs training and they have the proper amount of college credits( min 12 hours of the sciences) equaling to an AA or BA depending on the tech. They can sit for the test after one year working in a histology lab. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles.Embrey Sent: Wednesday, April 16, 2008 11:39 AM To: Webb, Dorothy L; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] certification I think the problem is that if you don't complete the process within the time period allowed then that route is closed to you and you must apply under a different route. Have her apply under the OJT route and take the exam that way. The credits for the OJT route don't have to be from a NAAClS accredited school. Simple transcripts from the old school should do the trick. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Wednesday, April 16, 2008 10:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] certification I have a histotech who has been in the field for 9 years, went to school for histology in 1999, (2 year Associates), passed the practical portion of the registry, but did not take the written within the 5 year allowance. She recently sent her $175 in and all paperwork to register for taking the test and has been told by ASCP that she is no longer eligible to take the test due to her credits not being from an accredited school. The school she attended was accredited and then taken over a few years ago by a University out of Chicago, so is now under new management and name. The present college can only accept "X" number of credits for transfer, even though it is the same program, same person in charge of the program ,etc. And, to top it all off, ASCP will not refund her entry fee nor hold it for her when she can take the exam. I find this hard to understand when there are so few schools around and techs are hard to find. This person is a very good tech and did not take the registry exam until we forced her hand, due to her being a single mom of twins with no financial support, etc. Has anyone ever heard of such a scenario and any ideas to help this tech, other than her trying to retake several college credits? Thanks for any ideas or suggestions to help out a fellow histotech!!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histobeach <@t> aol.com Thu Apr 17 13:29:51 2008 From: histobeach <@t> aol.com (histobeach@aol.com) Date: Thu Apr 17 13:30:12 2008 Subject: [Histonet] IHC Staining Variations Message-ID: <8CA6ED028A45C69-52C-95F@webmail-nd11.sysops.aol.com> Hello, We have an automated IHC stainer and the doctors are experiencing what they call, staining variations.? The whole tissue is not staining, just in parts, when areas of the tissues should be staining.? Has anyone experienced this problem and if so, what did you do to remedy it?? We were staining manually and used a Pap Pen before we switched to automation and I'm wondering if we are getting areas on the slide that are not being covered with the reagents.? We have 3 drop zones with 100ul of reagent dropping in each zone, for a total of 300ul per slide. I appreciate any feedback and help.? Trinity Whitecomb Histology Manager Lab System Works From mwich <@t> 7thwavelabs.com Thu Apr 17 13:34:43 2008 From: mwich <@t> 7thwavelabs.com (Michele Wich) Date: Thu Apr 17 13:34:51 2008 Subject: [Histonet] universal negative control Message-ID: <2264717ADC396742A0FF0AAB674F9A0D486C6F@7THWAVE-SERVER.7thwave.local> Does anyone know if it is acceptable to include just one "Universal" negative control with patient slides on which both polyclonal and monoclonal antibodies are being run? A previous manager (previous is the key word here) made us change all staining protocols to include only one universal control (I believe it was from Biocare) for each patient, no matter what antibodies were being tested on that patient, in a supposed effort to cut down on slide costs. Would this fly in a CAP inspection? This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer From LSebree <@t> uwhealth.org Thu Apr 17 13:39:27 2008 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Thu Apr 17 13:39:32 2008 Subject: [Histonet] universal negative control In-Reply-To: <2264717ADC396742A0FF0AAB674F9A0D486C6F@7THWAVE-SERVER.7thwave.local> Message-ID: Michele, We use the Universal Negative Control from Biocare and use it exactly how you describe. Our director and supervisor both seemed to think this would "fly". We have been inspected since that change but I'm not sure the topic came up. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michele Wich Sent: Thursday, April 17, 2008 1:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] universal negative control Does anyone know if it is acceptable to include just one "Universal" negative control with patient slides on which both polyclonal and monoclonal antibodies are being run? A previous manager (previous is the key word here) made us change all staining protocols to include only one universal control (I believe it was from Biocare) for each patient, no matter what antibodies were being tested on that patient, in a supposed effort to cut down on slide costs. Would this fly in a CAP inspection? This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dorothy.L.Webb <@t> HealthPartners.Com Thu Apr 17 13:46:04 2008 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Thu Apr 17 13:46:13 2008 Subject: [Histonet] certification In-Reply-To: <1CE1847DFEA0A647B1CCDE4108EA60A747A213@LTA3VS011.ees.hhs.gov> Message-ID: <0E394B648E5284478A6CCB78E5AFDA270563563C@hpes1.HealthPartners.int> That is correct!! She has the credits for an AA from Medical Institute of Minnesota, but now that it is Argosy University, they do not all transfer. Yes, if she hadn't put all of this off so long, this would not be a problem, but there are underlying circumstances that make this a unique case, in my eyes. And the $175 is a lot of money to a single Mom, as we all know histotechs employed at an urban hospital do not make a lot of money!!! I am just trying to get any possible help for her and feel slightly responsible as her supervisor! Thanks all who responded for trying to help out a fellow histotech!! -----Original Message----- From: Bartlett, Jeanine (CDC/CCID/NCZVED) [mailto:jqb7@cdc.gov] Sent: Thursday, April 17, 2008 1:02 PM To: Cheri Miller; Charles.Embrey; Webb, Dorothy L; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] certification I thought part of the problem was she could not get all of her credits to transfer because the school has since changed hands and will not accept all of them. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Thursday, April 17, 2008 1:38 PM To: 'Charles.Embrey'; 'Webb, Dorothy L'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] certification I agree with you Charles, I currently have 2 OJT techs training and they have the proper amount of college credits( min 12 hours of the sciences) equaling to an AA or BA depending on the tech. They can sit for the test after one year working in a histology lab. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles.Embrey Sent: Wednesday, April 16, 2008 11:39 AM To: Webb, Dorothy L; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] certification I think the problem is that if you don't complete the process within the time period allowed then that route is closed to you and you must apply under a different route. Have her apply under the OJT route and take the exam that way. The credits for the OJT route don't have to be from a NAAClS accredited school. Simple transcripts from the old school should do the trick. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Wednesday, April 16, 2008 10:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] certification I have a histotech who has been in the field for 9 years, went to school for histology in 1999, (2 year Associates), passed the practical portion of the registry, but did not take the written within the 5 year allowance. She recently sent her $175 in and all paperwork to register for taking the test and has been told by ASCP that she is no longer eligible to take the test due to her credits not being from an accredited school. The school she attended was accredited and then taken over a few years ago by a University out of Chicago, so is now under new management and name. The present college can only accept "X" number of credits for transfer, even though it is the same program, same person in charge of the program ,etc. And, to top it all off, ASCP will not refund her entry fee nor hold it for her when she can take the exam. I find this hard to understand when there are so few schools around and techs are hard to find. This person is a very good tech and did not take the registry exam until we forced her hand, due to her being a single mom of twins with no financial support, etc. Has anyone ever heard of such a scenario and any ideas to help this tech, other than her trying to retake several college credits? Thanks for any ideas or suggestions to help out a fellow histotech!!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From bakevictoria <@t> gmail.com Thu Apr 17 14:13:34 2008 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Thu Apr 17 14:13:40 2008 Subject: [Histonet] IHC Staining Variations In-Reply-To: <8CA6ED028A45C69-52C-95F@webmail-nd11.sysops.aol.com> References: <8CA6ED028A45C69-52C-95F@webmail-nd11.sysops.aol.com> Message-ID: <4f016b690804171213n79c30ffn85d84e5c40dda546@mail.gmail.com> Trinity, Are you using a Lab Vision stainer? If so are you also using their antigen retrieval system in conjunction with the stainer? Vikki Baker On 4/17/08, histobeach@aol.com wrote: > Hello, > > We have an automated IHC stainer and the doctors are experiencing what they call, staining variations.? The whole tissue is not staining, just in parts, when areas of the tissues should be staining.? > > Has anyone experienced this problem and if so, what did you do to remedy it?? We were staining manually and used a Pap Pen before we switched to automation and I'm wondering if we are getting areas on the slide that are not being covered with the reagents.? We have 3 drop zones with 100ul of reagent dropping in each zone, for a total of 300ul per slide. > > I appreciate any feedback and help.? > > Trinity Whitecomb > Histology Manager > Lab System Works > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From shive003 <@t> umn.edu Thu Apr 17 15:08:44 2008 From: shive003 <@t> umn.edu (Jan Shivers) Date: Thu Apr 17 15:09:04 2008 Subject: [Histonet] IHC Staining Variations References: <8CA6ED028A45C69-52C-95F@webmail-nd11.sysops.aol.com> Message-ID: <014401c8a0c6$d7175b20$a3065486@auxs.umn.edu> Occasionally in the past I'd have a problem with slides drying out while on the stainer. Suggestions: 1) Make sure that your stainer is level, especially the slide racks inside, if they are used in your type of machine. 2) Dilute your antibodies with a diluent which has protein stabilizers in it; the Ab/diluent won't dry out as fast. (I switched to Dako Antibody Diluent from my own in-house TBS buffer, but I'm sure there are other commercial products out there as well.). 3) Add 2% normal serum to your linking Ab (same species as your protein block prior to the primary antibody); this has several uses, but one is that the linking Ab won't dry out as fast. 4) Try reducing your incubation times, if possible. 5) Hydrophobic elements in tissue will tend to 'push' water off of the tissue, so some specific tissues are just more likely to dry out faster. If it's at all possible with your staining set-up, you could try adding additional reagent drops by hand (at the correct step, of course) when you notice a slide drying out in your machine. Jan Shivers Senior Scientist Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory shive003@umn.edu ----- Original Message ----- From: To: Sent: Thursday, April 17, 2008 1:29 PM Subject: [Histonet] IHC Staining Variations > Hello, > > We have an automated IHC stainer and the doctors are experiencing what > they call, staining variations.? The whole tissue is not staining, just in > parts, when areas of the tissues should be staining.? > > Has anyone experienced this problem and if so, what did you do to remedy > it?? We were staining manually and used a Pap Pen before we switched to > automation and I'm wondering if we are getting areas on the slide that are > not being covered with the reagents.? We have 3 drop zones with 100ul of > reagent dropping in each zone, for a total of 300ul per slide. > > I appreciate any feedback and help.? > > Trinity Whitecomb > Histology Manager > Lab System Works > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From marktarango <@t> gmail.com Thu Apr 17 15:22:41 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Thu Apr 17 15:22:45 2008 Subject: [Histonet] California techs In-Reply-To: References: Message-ID: <5b6eb13e0804171322w47400c19t7e2665cbc25e2148@mail.gmail.com> I asked to hear more about this a few days ago, but I didn't get any more information (not even off the list). I'm still wondering if its true. I even spent some time trying to find the law online. If anyone has any more info, please share! Mark On Thu, Apr 17, 2008 at 10:28 AM, Martin, Erin wrote: > Hi everyone, > > This came up a few days ago but I didn't see anything specific. Could > anyone in CA tell me what the story is regarding the governor's push for > licensure? > > Thanks > Erin > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Thu Apr 17 16:04:27 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Apr 17 16:04:31 2008 Subject: [Histonet] IHC Staining Variations In-Reply-To: <4f016b690804171213n79c30ffn85d84e5c40dda546@mail.gmail.com> Message-ID: <528449.39177.qm@web65702.mail.ac4.yahoo.com> Trinity: Are you adding Tween to your PBS? Not enough Tween can cause uneven staining. Ren? J. --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From JBower <@t> hei.org Thu Apr 17 16:32:05 2008 From: JBower <@t> hei.org (Bower, Jennifer) Date: Thu Apr 17 16:32:10 2008 Subject: [Histonet] fluorescent labeling of fixed, frozen tissue Message-ID: <87449E4A2B01DA47B29424CE5D6E0F83082562BB@hi0sml1.hei.org> Hey everyone! I have a big long question, I hope someone out there can help me. Ultimately, I need to do fluorescent labeling of formalin fixed, decalcified mouse cochlea. Right now, we are perfusion fixing mice with 10% NBF, dissecting out the bulla with the cochlea still within, decalcifying with EDTA in the microwave, then freezing the samples and cutting sections on the cryostat. So far, I have to let my slides air dry because if I put them straight into PBS, the sections fall off the slides. Also, everything fluoresces, I suspect due to autofluorescence, or maybe because of the formaldehyde? Isn't there an easier way? If the tissue is already fixed, why put the sections into acetone after cryosectioning? Do I need to do antigen retrieval? Should I try a pressure cooker method? There is also a lot of superstition surrounding the use of fluorescents with paraffin embedded tissue, no one seems to think it will work. Is anyone out there doing any sort of fluorescent labeling of fixed tissues? Thank you so much for any advice, I feel lost at this point. Thanks again, Jennifer From Susan.Weber2 <@t> va.gov Thu Apr 17 16:45:10 2008 From: Susan.Weber2 <@t> va.gov (Weber, Susan (VHACLE)) Date: Thu Apr 17 16:45:21 2008 Subject: [Histonet] IHC Staining Variations In-Reply-To: <8CA6ED028A45C69-52C-95F@webmail-nd11.sysops.aol.com> References: <8CA6ED028A45C69-52C-95F@webmail-nd11.sysops.aol.com> Message-ID: <16C83872A53F4346AA9C3A18E3A3AAB903F76D81@VHAV10MSGA1.v10.med.va.gov> Trinity- Are you using the Ventana Benchmark XT equipment? I recently started using this equipment and in our training class they told us that certain slides can be hydrophobic and using a Pap Pen can also effect the staining quality. I switched from Surgipath X slides to Fisher Superfrost/Plus slides for this very reason. I use the Shandon Double slide with the rings on them for our FITC stains and did not have a problem - the ring is on the underside of the slide, not the side you put the tissue on. Some of the slides which have "boxes" on them also affect staining quality (unless it is on the underside of the slide of course). If you are using the Ventana equipment, their Tech Support is very good and can help you. Even if your not using this equipment, I'd call the Tech support of your manufacturer and ask for their help. Susan M Weber HT(ASCP) Histology Supervisor Louis Stokes Cleveland VA Medical Center 10701 East Blvd Cleveland, Ohio 44106 (216) 791-3800 X6154 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histobeach@aol.com Sent: Thursday, April 17, 2008 2:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Staining Variations Hello, We have an automated IHC stainer and the doctors are experiencing what they call, staining variations.? The whole tissue is not staining, just in parts, when areas of the tissues should be staining.? Has anyone experienced this problem and if so, what did you do to remedy it?? We were staining manually and used a Pap Pen before we switched to automation and I'm wondering if we are getting areas on the slide that are not being covered with the reagents.? We have 3 drop zones with 100ul of reagent dropping in each zone, for a total of 300ul per slide. I appreciate any feedback and help.? Trinity Whitecomb Histology Manager Lab System Works _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Thu Apr 17 17:30:51 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Thu Apr 17 17:30:53 2008 Subject: [Histonet] fluorescent labeling of fixed, frozen tissue References: <87449E4A2B01DA47B29424CE5D6E0F83082562BB@hi0sml1.hei.org> Message-ID: <002101c8a0da$b16dd8e0$6501a8c0@DHXTS541> Many people do immunfluorescent staining on NBF perfused tissues but yours has unique problems involved. Bone, decalcification and then cryosections of boney parts. Unfortunately, fixed/decalcified bone frozen sections do not like staying on slides, even after air drying, which will make retrieval difficult too. You should not have to acetone fix after perfusion fixation, since the proteins are already cross linked, the acetone is probably not going to help that much with section retention. If you do fresh tissue, decalcified and then fixed with acetone, the section should stay on the slide surface. Air drying is one way to help, but may a gentle heat at 37C can help particularly if you use a protein coating, e.g. poly l lysine or possibly gelatin subbed slide to act as the glue for the section to slide surface. This was discussed on Histonet not too long ago. See cited references below. People who have used gelatin subbed slides can give more insight on its use for immunofluorescent staining. Is there any reason you can't perfuse fix, then decalcify and do paraffin embedded sections of the cochlea? The autofluorescence level will probably be the same as FF/EDTA decalcified/frozen sections anyway or is the antibody not going to work on FFPE/decalcified tissue. It seems to me if the antibody works on FF/decalcified frozen sections (section fall off slide), then it should work on FFPE (also decalcified) that stay on slide better, they why not do the latter and not cryosection? This way you can dry the slides longer to ensure section retention is improved although retrieval may have to be low temperature instead of harsher HIER methods. You probably are suffering from autofluorescence induced by the formaldehyde fixation. Formalin isn't fluorescing, it is inducing the autofluorescence. This will happen on tissues NBF fixed/decalcified for paraffin sections or for frozen sections. To read up on this, go to IHCworld website. You might be able reduce autofluorescence with 100 mM glycine in TRIS buffer, pH 7.4 (we also use Dulbeccos PBS at the same pH with 100 mM glycine. You rehydrate a paraffin section (frozen sections can be flooded with the solution) for 20 min at RT before proceeding to immunfluorescent immunostaining or apply it to a fixed frozen section. The other alternative although very pricey, is use Instrumedics Cryojane and then you can tape transfer any frozen section of bone, whether it is fixed or not to a slide surface. Unfixed, acetone fixed bone frozen sections should have minimal autofluorescence. Some people complain about the polymer on the slide but there are ways to deal with that problem. There is an excellent publication on doing decalcification/cryotomy for eGFP, and they may also do immunostaining too. Kusser and Randall, J Histochem Cytochem 51:5-14, Jan 2003. You can also decalcify unfixed bone, a long tedious protocol, snap freeze and then fix with acetone after the EDTA decalcification is complete. You will NOT be able to do microwave decalcification. this is done at 4C. Mori S et al. A new decalcifying technique for immunohistochemical studies of calcified tissue, especially applicable to cell surface marker demonstration. J. Histochem Cytochem. 36(1):111-4,1988. As for an easier way, we looked for them too, and ended up buying the Cryojane. Good luck Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Bower, Jennifer" To: Sent: Thursday, April 17, 2008 3:32 PM Subject: [Histonet] fluorescent labeling of fixed, frozen tissue Hey everyone! I have a big long question, I hope someone out there can help me. Ultimately, I need to do fluorescent labeling of formalin fixed, decalcified mouse cochlea. Right now, we are perfusion fixing mice with 10% NBF, dissecting out the bulla with the cochlea still within, decalcifying with EDTA in the microwave, then freezing the samples and cutting sections on the cryostat. So far, I have to let my slides air dry because if I put them straight into PBS, the sections fall off the slides. Also, everything fluoresces, I suspect due to autofluorescence, or maybe because of the formaldehyde? Isn't there an easier way? If the tissue is already fixed, why put the sections into acetone after cryosectioning? Do I need to do antigen retrieval? Should I try a pressure cooker method? There is also a lot of superstition surrounding the use of fluorescents with paraffin embedded tissue, no one seems to think it will work. Is anyone out there doing any sort of fluorescent labeling of fixed tissues? Thank you so much for any advice, I feel lost at this point. Thanks again, Jennifer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Malcolm.McCallum <@t> tamut.edu Thu Apr 17 17:44:12 2008 From: Malcolm.McCallum <@t> tamut.edu (Malcolm McCallum) Date: Thu Apr 17 17:47:37 2008 Subject: [Histonet] pentosidine question References: <8CA6ED028A45C69-52C-95F@webmail-nd11.sysops.aol.com> <16C83872A53F4346AA9C3A18E3A3AAB903F76D81@VHAV10MSGA1.v10.med.va.gov> Message-ID: I recently became familiar with a compound called Pentosidine. Anyone have leads on publications about its accumulation in organisms as they age? Thanks for the info! Malcolm L. McCallum Assistant Professor Department of Biological Sciences Texas A&M University Texarkana 2600 Robison Rd. Texarkana, TX 75501 O: 1-903-223-3134 H: 1-903-791-3843 Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html VISIT HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org A New Journal Published in Partnership with Partners in Amphibian and Reptile Conservation and the World Congress of Herpetology. Spring Teaching Schedule & Office Hours: Genetics: W 6:00 to 9:40pm Herpetology: TR 10:00-11:40am Histology: MW 1:00-2:40pm Seminar: T 2:30-3:30pm Office Hours: M: 3:30-5:00pm T: 11:40-1:00pm; 3:30-5:00pm W: 4:00-6:00pm "We live in a time when lemonade is made with artificial flavoring, and furnisher polish is made with fresh lemons." -Alfred E. Neuman -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Weber, Susan (VHACLE) Sent: Thu 4/17/2008 4:45 PM To: histobeach@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC Staining Variations Trinity- Are you using the Ventana Benchmark XT equipment? I recently started using this equipment and in our training class they told us that certain slides can be hydrophobic and using a Pap Pen can also effect the staining quality. I switched from Surgipath X slides to Fisher Superfrost/Plus slides for this very reason. I use the Shandon Double slide with the rings on them for our FITC stains and did not have a problem - the ring is on the underside of the slide, not the side you put the tissue on. Some of the slides which have "boxes" on them also affect staining quality (unless it is on the underside of the slide of course). If you are using the Ventana equipment, their Tech Support is very good and can help you. Even if your not using this equipment, I'd call the Tech support of your manufacturer and ask for their help. Susan M Weber HT(ASCP) Histology Supervisor Louis Stokes Cleveland VA Medical Center 10701 East Blvd Cleveland, Ohio 44106 (216) 791-3800 X6154 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histobeach@aol.com Sent: Thursday, April 17, 2008 2:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Staining Variations Hello, We have an automated IHC stainer and the doctors are experiencing what they call, staining variations.? The whole tissue is not staining, just in parts, when areas of the tissues should be staining.? Has anyone experienced this problem and if so, what did you do to remedy it?? We were staining manually and used a Pap Pen before we switched to automation and I'm wondering if we are getting areas on the slide that are not being covered with the reagents.? We have 3 drop zones with 100ul of reagent dropping in each zone, for a total of 300ul per slide. I appreciate any feedback and help.? Trinity Whitecomb Histology Manager Lab System Works _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From barbaraaalbert <@t> yahoo.com Thu Apr 17 18:42:17 2008 From: barbaraaalbert <@t> yahoo.com (Barbara Albert) Date: Thu Apr 17 18:42:22 2008 Subject: [Histonet] California techs In-Reply-To: <5b6eb13e0804171322w47400c19t7e2665cbc25e2148@mail.gmail.com> Message-ID: <415603.51834.qm@web63709.mail.re1.yahoo.com> I'm just guessing here, but the governor had a "Universal Health Care" plan that crashed and burned. Perhaps those regs were part of that plan. Barbara Albert UCSF Medical Center San Frrancisco Mark Tarango wrote: I asked to hear more about this a few days ago, but I didn't get any more information (not even off the list). I'm still wondering if its true. I even spent some time trying to find the law online. If anyone has any more info, please share! Mark On Thu, Apr 17, 2008 at 10:28 AM, Martin, Erin wrote: > Hi everyone, > > This came up a few days ago but I didn't see anything specific. Could > anyone in CA tell me what the story is regarding the governor's push for > licensure? > > Thanks > Erin > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From jcox90 <@t> yahoo.com Thu Apr 17 20:02:11 2008 From: jcox90 <@t> yahoo.com (Jill Cox) Date: Thu Apr 17 20:02:15 2008 Subject: Fwd: Re: [Histonet] California techs Message-ID: <288944.17567.qm@web56810.mail.re3.yahoo.com> I was told from a recruiter that in 2011 California will require a License just like Florida, NY and other License states. I don't know the criteria but it's probably similar to the others. Hope this helps? I was also told that it will raise the pay there!! Note: forwarded message attached. Jill Cox HT (ASCP) From cmiller <@t> physlab.com Fri Apr 18 07:40:49 2008 From: cmiller <@t> physlab.com (Cheri Miller) Date: Fri Apr 18 07:39:51 2008 Subject: [Histonet] certification In-Reply-To: <1CE1847DFEA0A647B1CCDE4108EA60A747A213@LTA3VS011.ees.hhs.gov> References: <0E394B648E5284478A6CCB78E5AFDA2705635627@hpes1.HealthPartners.int> <44780C571F28624DBB446DE55C4D733A1FE5A8@EXCHANGEBE1.carle.com> <000901c8a0b1$d25524b0$3d02a8c0@plab.local> <1CE1847DFEA0A647B1CCDE4108EA60A747A213@LTA3VS011.ees.hhs.gov> Message-ID: <000f01c8a151$6e2bb440$3d02a8c0@plab.local> If she has her 2 yr(AA) degree she doesn't have to take anymore classes. Just because the school has changed it doesn't mean her degree isn't valid anymore. All she needs is the 1 year practical experience before she sits for the exam. Why would she have to transfer credits?? If she completed and got the AA? Doesn't Make sense Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: Bartlett, Jeanine (CDC/CCID/NCZVED) [mailto:jqb7@cdc.gov] Sent: Thursday, April 17, 2008 1:02 PM To: Cheri Miller; Charles.Embrey; Webb, Dorothy L; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] certification I thought part of the problem was she could not get all of her credits to transfer because the school has since changed hands and will not accept all of them. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Thursday, April 17, 2008 1:38 PM To: 'Charles.Embrey'; 'Webb, Dorothy L'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] certification I agree with you Charles, I currently have 2 OJT techs training and they have the proper amount of college credits( min 12 hours of the sciences) equaling to an AA or BA depending on the tech. They can sit for the test after one year working in a histology lab. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles.Embrey Sent: Wednesday, April 16, 2008 11:39 AM To: Webb, Dorothy L; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] certification I think the problem is that if you don't complete the process within the time period allowed then that route is closed to you and you must apply under a different route. Have her apply under the OJT route and take the exam that way. The credits for the OJT route don't have to be from a NAAClS accredited school. Simple transcripts from the old school should do the trick. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Wednesday, April 16, 2008 10:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] certification I have a histotech who has been in the field for 9 years, went to school for histology in 1999, (2 year Associates), passed the practical portion of the registry, but did not take the written within the 5 year allowance. She recently sent her $175 in and all paperwork to register for taking the test and has been told by ASCP that she is no longer eligible to take the test due to her credits not being from an accredited school. The school she attended was accredited and then taken over a few years ago by a University out of Chicago, so is now under new management and name. The present college can only accept "X" number of credits for transfer, even though it is the same program, same person in charge of the program ,etc. And, to top it all off, ASCP will not refund her entry fee nor hold it for her when she can take the exam. I find this hard to understand when there are so few schools around and techs are hard to find. This person is a very good tech and did not take the registry exam until we forced her hand, due to her being a single mom of twins with no financial support, etc. Has anyone ever heard of such a scenario and any ideas to help this tech, other than her trying to retake several college credits? Thanks for any ideas or suggestions to help out a fellow histotech!!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From Clarissa.Wright <@t> med.navy.mil Fri Apr 18 08:59:00 2008 From: Clarissa.Wright <@t> med.navy.mil (Wright, Clarissa B CIV) Date: Fri Apr 18 08:59:08 2008 Subject: [Histonet] Bond Users Message-ID: <8072C1D1C2865C49B7F0A95FC47B7646AABEE6@NMCSD-EX-VS-01.nmed.ds.med.navy.mil> Good Morning Fellow Histonetters, I would like for users of the "Bond Immuno Stainer" to contact me offline. We are evaluating the stainer now and I have a couple of questions about its performance in full operational mode and . I would especially like to hear from military facilities. . If anyone else is interested in the results of my questions, I will compile and post them on the Histonet. Thank you for any responses. Clarissa.wright@med.navy.mil 619 532 9247 Clarissa Wright, HT Naval Medical Center San Diego From JMacDonald <@t> mtsac.edu Fri Apr 18 10:46:25 2008 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Apr 18 10:46:37 2008 Subject: [Histonet] certification In-Reply-To: <0E394B648E5284478A6CCB78E5AFDA270563563C@hpes1.HealthPartners.int> Message-ID: If she does have the credits for an AA, and it includes the necessary (12 semester units of biology/chemistry she can qualify under route 2. Route 2: Associate degree or at least 60 semester hours (90 quarter hours) of academic credit from a regionally accredited college/university, with a combination of 12 semester hours (18 quarter hours) of biology and chemistry, AND one year full time acceptable experience in histopathology in the U.S., Canada or a CAP/The Joint Commission (JCAHO)/AABB accredited laboratory within the last ten years under the supervision of a pathologist (certified by the American Board of Pathology in Anatomic Pathology), or an appropriately board certified medical scientist. Have her contact the ASCP to correct the route in which she is going to take the exam and have her transcripts sent to the ASCP. The units do not need to transfer. She just need proof that she took the classes and passed them. I cannot guarantee that they will not make her reapply under route 2,but it is worth a try. Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu "Webb, Dorothy L" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/17/2008 11:50 AM To "Bartlett, Jeanine (CDC/CCID/NCZVED)" , Cheri Miller , "Charles.Embrey" , "Webb, Dorothy L" , histonet@lists.utsouthwestern.edu cc Subject RE: [Histonet] certification That is correct!! She has the credits for an AA from Medical Institute of Minnesota, but now that it is Argosy University, they do not all transfer. Yes, if she hadn't put all of this off so long, this would not be a problem, but there are underlying circumstances that make this a unique case, in my eyes. And the $175 is a lot of money to a single Mom, as we all know histotechs employed at an urban hospital do not make a lot of money!!! I am just trying to get any possible help for her and feel slightly responsible as her supervisor! Thanks all who responded for trying to help out a fellow histotech!! -----Original Message----- From: Bartlett, Jeanine (CDC/CCID/NCZVED) [mailto:jqb7@cdc.gov] Sent: Thursday, April 17, 2008 1:02 PM To: Cheri Miller; Charles.Embrey; Webb, Dorothy L; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] certification I thought part of the problem was she could not get all of her credits to transfer because the school has since changed hands and will not accept all of them. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Thursday, April 17, 2008 1:38 PM To: 'Charles.Embrey'; 'Webb, Dorothy L'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] certification I agree with you Charles, I currently have 2 OJT techs training and they have the proper amount of college credits( min 12 hours of the sciences) equaling to an AA or BA depending on the tech. They can sit for the test after one year working in a histology lab. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles.Embrey Sent: Wednesday, April 16, 2008 11:39 AM To: Webb, Dorothy L; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] certification I think the problem is that if you don't complete the process within the time period allowed then that route is closed to you and you must apply under a different route. Have her apply under the OJT route and take the exam that way. The credits for the OJT route don't have to be from a NAAClS accredited school. Simple transcripts from the old school should do the trick. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Wednesday, April 16, 2008 10:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] certification I have a histotech who has been in the field for 9 years, went to school for histology in 1999, (2 year Associates), passed the practical portion of the registry, but did not take the written within the 5 year allowance. She recently sent her $175 in and all paperwork to register for taking the test and has been told by ASCP that she is no longer eligible to take the test due to her credits not being from an accredited school. The school she attended was accredited and then taken over a few years ago by a University out of Chicago, so is now under new management and name. The present college can only accept "X" number of credits for transfer, even though it is the same program, same person in charge of the program ,etc. And, to top it all off, ASCP will not refund her entry fee nor hold it for her when she can take the exam. I find this hard to understand when there are so few schools around and techs are hard to find. This person is a very good tech and did not take the registry exam until we forced her hand, due to her being a single mom of twins with no financial support, etc. Has anyone ever heard of such a scenario and any ideas to help this tech, other than her trying to retake several college credits? Thanks for any ideas or suggestions to help out a fellow histotech!!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MElliott <@t> mrl.ubc.ca Fri Apr 18 12:15:10 2008 From: MElliott <@t> mrl.ubc.ca (Mark Elliott) Date: Fri Apr 18 12:16:04 2008 Subject: [Histonet] Envision G2 on Frozen Tissues In-Reply-To: <82178084.819@mail.mrl.ubc.ca> References: <82178084.819@mail.mrl.ubc.ca> Message-ID: <480874AD.11C6.00D6.0@mrl.ubc.ca> I am looking for some information on a problem we are having. Has anyone used the Envision G2 system from DAKO on frozen tissues? We are trying to work it up on frozens and are getting discrete positive looking cells with our IgG control. We been working with DAKO on resolving this and have tried everything we can think of to eliminate this staining but with no luck. We are working with mouse primaries on human tissue. The staining occurs on tonsil, tumor and lung tissue-haven't tried other tissues as we work only with these. We have diluted our IgG out to extremely low concentrations and still get extremely bright staining. When working with the APAAP System on frozens we get no background staining with the IgG. When we do it on FFPE tissue's there is no staining on our controls-they are clean. We are working with the Alk Phos version of the kit since we are working with lung tissue we try ans avoid using Peroxidase. Any suggestions/explanations greatly appreciated? DAKO is at a loss as well as to what is happening. Thanks Mark ***CONFIDENTIALITY NOTICE*** This electronic message is intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From Erin.Martin <@t> ucsf.edu Fri Apr 18 12:27:36 2008 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Fri Apr 18 12:29:40 2008 Subject: [Histonet] California techs, again Message-ID: I think Barbara might be right - maybe the license issue was part of Arnold's failed health care plan. It is weird that the out-of-state techs have heard more about this than the in-state techs! If any of the other CA techs hear anything, could you please let me know? I can't find anything about this... Erin From bmbow <@t> seagen.com Fri Apr 18 12:47:20 2008 From: bmbow <@t> seagen.com (Brianna Mbow) Date: Fri Apr 18 12:47:30 2008 Subject: [Histonet] commercial antibody diluents w/ surfactants Message-ID: <9916263273C7B945BE6FF9ACBDD6C50A035919AE@SGEXCH.corp.seagen.com> Hello, In my previous position, I prepared all my own antibody solutions for fluorescent staining myself with varying levels of blocking serum and triton-x for permeabilization. I'm now working in a lab that uses commercial antibody diluents and an autostainer with its own washing solution. Both the diluent and wash solution contain surfactant, but the concentration is not listed. I'm just wondering if anyone knows if the amount is sufficient for permeabilization in fluorescent staining. So far in the lab we have only really been doing IHC and they have never added triton-x to their solutions. Also, out of general curiosity, is there a reason for needing more permeabilization in IF compared to IHC staining? Is it because of the size of fluorophore molecules? Any ideas would be much appreciated. Thank you, Brianna Mbow Antibody Technologies Seattle Genetics Seattle, WA From Norm.Burnham <@t> propath.com Thu Apr 17 18:57:14 2008 From: Norm.Burnham <@t> propath.com (Norm Burnham) Date: Fri Apr 18 12:53:30 2008 Subject: [Histonet] Pathology Assistant grossing productivity Message-ID: Hello all, Does anyone have guidelines on how many cases and specimens a good, competent Pathologist Assistant should be able to gross per 8 hour day? We see about 20-25% hospital cases of all types and about 75-80% outpatient biopsies. Thanks! Norm Burnham ______________________________________________________________________________ This e-mail may contain confidential or privileged information. If you think you have received this e-mail in error, please advise the sender by reply e-mail and then delete this e-mail immediately. From Norm.Burnham <@t> propath.com Thu Apr 17 19:32:25 2008 From: Norm.Burnham <@t> propath.com (Norm Burnham) Date: Fri Apr 18 12:53:32 2008 Subject: [Histonet] Productivity of Pathology Assistants Message-ID: Hello all, Does anyone have guidelines on how many cases and specimens a good, competent Pathologist Assistant should be able to gross per 8 hour day? We see about 20-25% hospital cases of all types and about 75-80% outpatient biopsies. Thanks! Norm Burnham ______________________________________________________________________________ This e-mail may contain confidential or privileged information. If you think you have received this e-mail in error, please advise the sender by reply e-mail and then delete this e-mail immediately. From Norm.Burnham <@t> propath.com Fri Apr 18 10:33:14 2008 From: Norm.Burnham <@t> propath.com (Norm Burnham) Date: Fri Apr 18 12:53:34 2008 Subject: [Histonet] Pathology Assistant grossing productivity Message-ID: Hello all, Does anyone have guidelines on the number of cases and the number of specimens a good, competent Pathologist Assistant should be able to gross per 8 hour day? Our case mix is something like 20-25% hospital cases of all types and about 75-80% outpatient biopsies. Thanks! Norm Burnham ______________________________________________________________________________ This e-mail may contain confidential or privileged information. If you think you have received this e-mail in error, please advise the sender by reply e-mail and then delete this e-mail immediately. From Norm.Burnham <@t> propath.com Fri Apr 18 12:40:05 2008 From: Norm.Burnham <@t> propath.com (Norm Burnham) Date: Fri Apr 18 12:53:36 2008 Subject: [Histonet] Pathology Assistant grossing productivity Message-ID: Hello all, Does anyone have guidelines on the number of cases and the number of specimens a good, competent Pathologist Assistant should be able to gross per 8 hour day? Our case mix is something like 20-25% hospital cases of all types and about 75-80% outpatient biopsies. Thanks! Norm Burnham ______________________________________________________________________________ This e-mail may contain confidential or privileged information. If you think you have received this e-mail in error, please advise the sender by reply e-mail and then delete this e-mail immediately. From jessgrocki <@t> yahoo.com Fri Apr 18 13:00:24 2008 From: jessgrocki <@t> yahoo.com (Jessica Piche) Date: Fri Apr 18 13:00:35 2008 Subject: [Histonet] Tissue Tek VIP E300 4896 Message-ID: <407265.45619.qm@web82006.mail.mud.yahoo.com> Hello, I was wondering if anyone out there might have an extra service manual for a Tissue Tek VIP E300 4896 floor model? Somehow ours has walked away from the lab. Thanks, Jessica PIche-Grocki, HT(ASCP) Waterbury Hospital From gayle.callis <@t> bresnan.net Fri Apr 18 13:11:00 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Fri Apr 18 13:11:01 2008 Subject: [Histonet] commercial antibody diluents w/ surfactants References: <9916263273C7B945BE6FF9ACBDD6C50A035919AE@SGEXCH.corp.seagen.com> Message-ID: <001801c8a17f$8eca4bc0$6501a8c0@DHXTS541> Question: are you planning to do immunofluorescent staining even though you are now doing only enzyme immunohistochemistry with these reagents? The size of the fluorophore molecules is not a factor but there are some other considerations when doing IF. Gayle M. Callis HTL/HT/MT(ASCP0 Bozeman MT 59715 ----- Original Message ----- From: "Brianna Mbow" To: Sent: Friday, April 18, 2008 11:47 AM Subject: [Histonet] commercial antibody diluents w/ surfactants Hello, In my previous position, I prepared all my own antibody solutions for fluorescent staining myself with varying levels of blocking serum and triton-x for permeabilization. I'm now working in a lab that uses commercial antibody diluents and an autostainer with its own washing solution. Both the diluent and wash solution contain surfactant, but the concentration is not listed. I'm just wondering if anyone knows if the amount is sufficient for permeabilization in fluorescent staining. So far in the lab we have only really been doing IHC and they have never added triton-x to their solutions. Also, out of general curiosity, is there a reason for needing more permeabilization in IF compared to IHC staining? Is it because of the size of fluorophore molecules? Any ideas would be much appreciated. Thank you, Brianna Mbow Antibody Technologies Seattle Genetics Seattle, WA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charles.Embrey <@t> carle.com Fri Apr 18 13:47:42 2008 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Fri Apr 18 13:51:21 2008 Subject: [Histonet] Pathology Assistant grossing productivity In-Reply-To: References: Message-ID: <44780C571F28624DBB446DE55C4D733A1FE5AC@EXCHANGEBE1.carle.com> Hi Norm, With that kind of mix most places I know of or have worked, staff one PA per 15,000 to 17,000 cases annually. I hope that helps. Charles Embrey, PA(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Norm Burnham Sent: Thursday, April 17, 2008 6:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathology Assistant grossing productivity Hello all, Does anyone have guidelines on how many cases and specimens a good, competent Pathologist Assistant should be able to gross per 8 hour day? We see about 20-25% hospital cases of all types and about 75-80% outpatient biopsies. Thanks! Norm Burnham ________________________________________________________________________ ______ This e-mail may contain confidential or privileged information. If you think you have received this e-mail in error, please advise the sender by reply e-mail and then delete this e-mail immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Apr 18 15:03:30 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Apr 18 15:03:34 2008 Subject: [Histonet] Pathology Assistant grossing productivity In-Reply-To: Message-ID: <743809.35361.qm@web65709.mail.ac4.yahoo.com> With help of an assistant to fill the cassettes (that have been previously numbered), just dictating, the PA should be able to dictate 100 units in 1.7 hours = or 384 units in 8 hours with a time utilization coefficient of 0.8 Ren? J. Norm Burnham wrote: Hello all, Does anyone have guidelines on how many cases and specimens a good, competent Pathologist Assistant should be able to gross per 8 hour day? We see about 20-25% hospital cases of all types and about 75-80% outpatient biopsies. Thanks! Norm Burnham ______________________________________________________________________________ This e-mail may contain confidential or privileged information. If you think you have received this e-mail in error, please advise the sender by reply e-mail and then delete this e-mail immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From bmbow <@t> seagen.com Fri Apr 18 15:19:25 2008 From: bmbow <@t> seagen.com (Brianna Mbow) Date: Fri Apr 18 15:19:30 2008 Subject: [Histonet] commercial antibody diluents w/ surfactants In-Reply-To: <001801c8a17f$8eca4bc0$6501a8c0@DHXTS541> Message-ID: <9916263273C7B945BE6FF9ACBDD6C50A035919AF@SGEXCH.corp.seagen.com> Yes, I am planning on doing immunofluorescence staining and would like to adapt a protocol to the autostainer, which has the built in washing fluid. I previously did fluorescence staining on the bench, and I know I always used triton-x for permeabilization. I'm now just wondering if I begin with trying the commercial antibody diluent (in keeping with the general practice of the lab), is the surfactant already in the solution enough for permeabilization or would I need added triton-x? And if I would need to add more, why is this not necessary with immunohistochemical staining? Thanks again, Brianna Mbow Antibody Technologies Seattle Genetics Seattle, WA -----Original Message----- From: Gayle Callis [mailto:gayle.callis@bresnan.net] Sent: Friday, April 18, 2008 11:11 AM To: Brianna Mbow; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] commercial antibody diluents w/ surfactants Question: are you planning to do immunofluorescent staining even though you are now doing only enzyme immunohistochemistry with these reagents? The size of the fluorophore molecules is not a factor but there are some other considerations when doing IF. Gayle M. Callis HTL/HT/MT(ASCP0 Bozeman MT 59715 ----- Original Message ----- From: "Brianna Mbow" To: Sent: Friday, April 18, 2008 11:47 AM Subject: [Histonet] commercial antibody diluents w/ surfactants Hello, In my previous position, I prepared all my own antibody solutions for fluorescent staining myself with varying levels of blocking serum and triton-x for permeabilization. I'm now working in a lab that uses commercial antibody diluents and an autostainer with its own washing solution. Both the diluent and wash solution contain surfactant, but the concentration is not listed. I'm just wondering if anyone knows if the amount is sufficient for permeabilization in fluorescent staining. So far in the lab we have only really been doing IHC and they have never added triton-x to their solutions. Also, out of general curiosity, is there a reason for needing more permeabilization in IF compared to IHC staining? Is it because of the size of fluorophore molecules? Any ideas would be much appreciated. Thank you, Brianna Mbow Antibody Technologies Seattle Genetics Seattle, WA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Heather.D.Renko <@t> osfhealthcare.org Fri Apr 18 16:18:57 2008 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Fri Apr 18 16:19:17 2008 Subject: [Histonet] re;Histology position in Beautiful Rockford, Illinois Message-ID: <40026EDDE64CDA47AB382C52619ACD3C0A09DEA1@pmc-rfd-mx01.intranet.osfnet.org> I have just posted a position for a full time Histotechnician or Histotechnologist here in beautiful Rockford, Illinois. The position is M-Sat forty hours/eight hour days with rotating Saturday s. Good people to work with and three of the most respectful pathologists you have ever met. We have the state of the art automation in every way and perform approximately 10,000 surgical cases a year with onsite IHC's and non-gyne cytology. The individual should have experience with embedding, microtomy, special stains, IHC, and frozen sectioning. Must be ASCP certified and a strong commitment to uphold our mission. Please feel free to personally contact me for additional information. www.Osfhealthcare.org Heather D. Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 Direct: 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From gayle.callis <@t> bresnan.net Fri Apr 18 17:37:13 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Fri Apr 18 17:37:29 2008 Subject: [Histonet] Cochlea frozen section difficulty and a publication that may help Message-ID: <000a01c8a1a4$c16d1880$6501a8c0@DHXTS541> You may find this publication the answer to your problems. Brain Research Protocols Volume 6, Issue 3, February 2001, Pages 159-166 Cryoembedding and sectioning of cochleas for immunocytochemistry and in situ hybridization Donna S. Whitlon, Renee Szakaly, Mary A. Greiner Abstract Current emphasis on biochemical and molecular aspects of cochlear anatomy underscores the necessity for high quality cryostat sections of the inner ear. The large volume of fluid space within the cochlea makes cryoembedding and sectioning of the organ more problematic than that of other, more homogeneous tissues. Our method for cryoembedding of cochleas for immunocytochemistry and in situ hybridization uses slow infiltration with increasing concentrations of sucrose followed by degassed embedding medium before final orientation and freezing. This method permits high quality cryosections to be cut which preserve overall structure and cellular resolution. Author Keywords: Cryosections; Cochlea; Immunocytochemistry; In situ hybridization; Hair cell; Mouse Neuroscience classification codes: Cellular and molecular biology, Staining, tracing, and imaging techniques Good luck, Gayle M. Callis HTL/HT/MT(ASCP) From san.htin <@t> yahoo.com Fri Apr 18 21:01:41 2008 From: san.htin <@t> yahoo.com (San Tin) Date: Fri Apr 18 21:01:45 2008 Subject: [Histonet] ISH protocol Message-ID: <761824.31158.qm@web55802.mail.re3.yahoo.com> Dear All, I have some questions with the usage of positive RNA probe in the usage of EBV detection. I was told to do positive RNA probe together with EBER, kappa and lambda ISH on the test tissue in addition to positive controls to make sure that the RNA is properly preserve on the fixation provcess. Here the problems is do they all need to be the same protocol for every probes as my kappa, lambda and EBER are using different hybridization temperature and time? Please kindly advice. San _________________________________________________________________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. [1]Try it now. References 1. http://us.rd.yahoo.com/evt=51733/*http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ From MVaughan4 <@t> ucok.edu Sat Apr 19 14:29:35 2008 From: MVaughan4 <@t> ucok.edu (MVaughan4@ucok.edu) Date: Sat Apr 19 14:30:03 2008 Subject: [Histonet] Pentosidine publications Message-ID: Malcolm, Bill Radke at UCO has done some work with pentosidine. He could give you some leads. Here are a couple from him, though they don't necessarily deal with aging: Title: Stability of pentosidine concentrations in museum study skins Author(s): Fallon JA, Radke WJ, Klandorf H Source: AUK Volume: 123 Issue: 1 Pages: 148-152 Published: JAN 2006 Times Cited: 1 2. Title: Skin tissue pentosidine in cormorants and study skin preparations of Ruffed grouse (Bonasa umbellus) Author(s): Klandorf H, Fallon JA, Radke WJ, et al. Source: FASEB JOURNAL Volume: 19 Issue: 4 Pages: A216-A216 Part: Part 1 Suppl. S Supplement: Part 1 Suppl. S Published: MAR 4 2005 Times Cited: 0 ----------------------------------------- **CONFIDENTIALITY** -This email (including any attachments) may contain confidential, proprietary and privileged information. Any unauthorized disclosure or use of this information is prohibited. From tahilburn <@t> hotmail.com Sat Apr 19 22:39:56 2008 From: tahilburn <@t> hotmail.com (Tressa Hilburn) Date: Sat Apr 19 22:39:59 2008 Subject: [Histonet] please drop me from the histonet list, thank you, tressa hilburn Message-ID: please drop me from the histo net llist, thank you, tressa hilburn From kemlo <@t> f2s.com Sun Apr 20 05:33:29 2008 From: kemlo <@t> f2s.com (kemlo) Date: Sun Apr 20 05:33:44 2008 Subject: [Histonet] Blood science Mananger Message-ID: Position is soon to become vacant at Weston General Hospital at Weston Super Mare, Somerset, UK., for Blood Science Manager at 8A/ 8B grade depending on applicant. This is a multidiscipliany Laboratory with CPA and On Call service which on average realises about ?12k on top of basic. Weston Super Mare is by the sea but that disappears to Wales every 12 hours and is a tad muddy, but the ciders OK. Kemlo Rogerson Acting Divisional Mananger Diagnostics Weston General Hospital North Somerst 01934 647057 From Shirley_PHUA <@t> hsa.gov.sg Sun Apr 20 13:03:46 2008 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Sun Apr 20 13:04:06 2008 Subject: [Histonet] Shirley Phua is out-of-office ... Message-ID: I will be out of the office from 21-04-2008 to 22-04-2008. I'll be away doing KEN training course on 21 & 22 April 2008. Pathologists: I will process your requests when I return. If urgent, please forward your email to Henry_Kyaw@hsa.gov.sg From liz <@t> premierlab.com Sun Apr 20 13:52:09 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Sun Apr 20 13:52:12 2008 Subject: [Histonet] need help with PAP stain Message-ID: Hello everyone I need to run a pap stain on some saliva samples and I don't know where to begin. I know this seems like a silly question, but I have done some searching on the net and there are quite a few protocols with different reagents. Its not a large project for us, I only need to run about 30 to 40 slides and was hoping to purchase a kit that would contain the hematoxylin, OG-6 and EA-50 but the only one I can find is the one from thermo the rapid chrome pap staining kit but I can't seem to find a vendor that sells it. If possible I would like to purchase pints of these reagents rather than gallons. Any advice as to what reagents to purchase and a protocol would be helpful. Also advice on what the stain should look like would help too. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 From c.malcontenti-wilson <@t> unimelb.edu.au Sun Apr 20 19:20:17 2008 From: c.malcontenti-wilson <@t> unimelb.edu.au (Cathy Malcontenti-Wilson) Date: Sun Apr 20 19:20:38 2008 Subject: [Histonet] Frozen sample storage Message-ID: <452655D3CC46464485FE5107C8723F2A01FB1C07@IS-EX-BEV1.unimelb.edu.au> Hi All, I would like some opinions on the storage of oct embedded tissues. These samples of animal tissues are embedded into 1 x 1cm pieces of oct and sit in a vial containing isopentane, and have been stored at -20 degrees for around 6 months. The freezer is only used for long term storage and so isn't opened very often (perhaps once per month). In the past I have stored my oct embedded tissues like this at -70 degrees. What would be your opinion of the antigenicity in these tissues if they were to be used for immunohistochemistry?? Thanks Cathy From godsgalnow <@t> aol.com Mon Apr 21 06:35:30 2008 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Mon Apr 21 06:35:37 2008 Subject: [Histonet] scanned reqs Message-ID: <8CA71BAEFCBFD8C-FAC-38F@Webmail-mg03.sim.aol.com> I have a question and I am hoping someone out there can help me with it... With the new age of technology, is anyone out there scanning the requisitions and accompanying paperwork?? Do you still need to keep the original? We are looking for a way to conserve space by scanning all of the paperwork after the case is signed out...however we can't all seem to agree on whether this will actually save space because we don't know if we still need to keep the original. Anyone from the regulatory committees, please chime in. Roxanne From godsgalnow <@t> aol.com Mon Apr 21 06:37:49 2008 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Mon Apr 21 06:37:58 2008 Subject: [Histonet] molecular pathology Message-ID: <8CA71BB431D995A-FAC-3AD@Webmail-mg03.sim.aol.com> How many histology labs are doing molecular pathology testing?? Like FISH, break aparts, PCR, etc. Who do you have performing the tests?? Are the Histotechs doing it, or have you hired a cytogenetics tech?? Or someone else? Roxanne From pieronelva01 <@t> bigpond.com Mon Apr 21 07:21:56 2008 From: pieronelva01 <@t> bigpond.com (Piero Nelva) Date: Mon Apr 21 07:22:05 2008 Subject: [Histonet] scanned reqs References: <8CA71BAEFCBFD8C-FAC-38F@Webmail-mg03.sim.aol.com> Message-ID: <000a01c8a3aa$4a4fa300$6371be7c@pentium4> WE routinely scan all path request slips and keep the originals for 6 months. THis also applies to diagrams made at grossing and frozen section reports given to surgeons. THis satisfies our accreditation agency, NATA. Regards Piero Nelva Anatomical Pathology Monash Medical Centre Australia ----- Original Message ----- From: To: Sent: Monday, April 21, 2008 9:35 PM Subject: [Histonet] scanned reqs >I have a question and I am hoping someone out there can help me with it... > > With the new age of technology, is anyone out there scanning the > requisitions and accompanying paperwork?? Do you still need to keep the > original? > > We are looking for a way to conserve space by scanning all of the > paperwork after the case is signed out...however we can't all seem to > agree on whether this will actually save space because we don't know if we > still need to keep the original. > > Anyone from the regulatory committees, please chime in. > > Roxanne > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG. > Version: 7.5.524 / Virus Database: 269.23.2/1388 - Release Date: 4/20/2008 > 3:01 PM > > From rjbuesa <@t> yahoo.com Mon Apr 21 07:25:25 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Apr 21 07:25:29 2008 Subject: [Histonet] Frozen sample storage In-Reply-To: <452655D3CC46464485FE5107C8723F2A01FB1C07@IS-EX-BEV1.unimelb.edu.au> Message-ID: <160866.56862.qm@web65715.mail.ac4.yahoo.com> They should be reactive. Ren? J. Cathy Malcontenti-Wilson wrote: http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From rjbuesa <@t> yahoo.com Mon Apr 21 07:29:00 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Apr 21 07:29:07 2008 Subject: [Histonet] scanned reqs In-Reply-To: <8CA71BAEFCBFD8C-FAC-38F@Webmail-mg03.sim.aol.com> Message-ID: <32241.21695.qm@web65712.mail.ac4.yahoo.com> For legal purposes, a hard copy should be kept. Where you keep it (in house or with an outside company dedicated to filing reports, slides, etc), is another issue. Ren? J. godsgalnow@aol.com wrote: --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From rjbuesa <@t> yahoo.com Mon Apr 21 07:33:53 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Apr 21 07:34:00 2008 Subject: [Histonet] molecular pathology In-Reply-To: <8CA71BB431D995A-FAC-3AD@Webmail-mg03.sim.aol.com> Message-ID: <44843.18163.qm@web65703.mail.ac4.yahoo.com> At least 5% of labs perform FISH, but PCR is usually sent to specialized facilities. When done in the lab a specially trained HTL takes care of the FISH tests. Ren? J. godsgalnow@aol.com wrote: --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From Vickroy.Jim <@t> mhsil.com Mon Apr 21 08:27:13 2008 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Mon Apr 21 08:30:30 2008 Subject: [Histonet] immuno controls for cell blocks Message-ID: <24A4826E8EF0964D86BC5317306F58A50680FF78BC@mmc-mail.ad.mhsil.com> We are currently using a bank of formalin fixed tissue controls for our immunohistochemical stains. We have done a short study to show that the reactivity and specificity of our immunostains on the cellblocks, hardening in 95%ETOH and then transferred in formalin, is not different than our regularly formalin fixed tissue. As I read the new CAP checklist this is what we are required to do. Now a cytopathologist wants me to maintain a set of controls only for his cellblocks. How is everybody else handling this situation? Jim Vickroy Memorial Medical Center Springfield, Illinois. This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From SDrew <@t> uwhealth.org Mon Apr 21 08:40:54 2008 From: SDrew <@t> uwhealth.org (Drew Sally A.) Date: Mon Apr 21 08:41:07 2008 Subject: [Histonet] immuno controls for cell blocks In-Reply-To: <24A4826E8EF0964D86BC5317306F58A50680FF78BC@mmc-mail.ad.mhsil.com> Message-ID: <3F328377AF4E23438E78234752652CE105D526D0@uwhis-xchng7.uwhis.hosp.wisc.edu> We are only using routinely processed FFPE tissues as controls for cell blocks. Depending on what antibodies you might run on cell blocks, I would think it difficult and impractical to try to obtain enough material to create cell block controls... Sally Ann Drew, MT(ASCP) IHC/ISH Laboratory University of Wisconsin Hosp. & Clinics Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Monday, April 21, 2008 8:27 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] immuno controls for cell blocks We are currently using a bank of formalin fixed tissue controls for our immunohistochemical stains. We have done a short study to show that the reactivity and specificity of our immunostains on the cellblocks, hardening in 95%ETOH and then transferred in formalin, is not different than our regularly formalin fixed tissue. As I read the new CAP checklist this is what we are required to do. Now a cytopathologist wants me to maintain a set of controls only for his cellblocks. How is everybody else handling this situation? Jim Vickroy Memorial Medical Center Springfield, Illinois. This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victor <@t> pathology.washington.edu Mon Apr 21 09:07:59 2008 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Mon Apr 21 09:08:09 2008 Subject: [Histonet] scanned reqs In-Reply-To: <8CA71BAEFCBFD8C-FAC-38F@Webmail-mg03.sim.aol.com> References: <8CA71BAEFCBFD8C-FAC-38F@Webmail-mg03.sim.aol.com> Message-ID: <480C9FBF.1090107@pathology.washington.edu> We keep the original for a short period of time before tossing. Make sure you have lots of disk space. We store all images (gross, micro and document) in one table. In two years that table grew from nothing to well over 100GB. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. godsgalnow@aol.com wrote: > I have a question and I am hoping someone out there can help me with it... > > With the new age of technology, is anyone out there scanning the requisitions and accompanying paperwork?? Do you still need to keep the original? > > We are looking for a way to conserve space by scanning all of the paperwork after the case is signed out...however we can't all seem to agree on whether this will actually save space because we don't know if we still need to keep the original. > > Anyone from the regulatory committees, please chime in. > > Roxanne > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gayle.callis <@t> bresnan.net Mon Apr 21 09:26:11 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Mon Apr 21 09:26:12 2008 Subject: [Histonet] Frozen sample storage References: <452655D3CC46464485FE5107C8723F2A01FB1C07@IS-EX-BEV1.unimelb.edu.au> Message-ID: <002901c8a3bb$a5ff1a30$6501a8c0@DHXTS541> Cathy, -70 or lower is always better, but if you can't access a lower temperature freezer, it may be in your best interest to test antigenicity more frequently. We use -80C and have found, with our murine tissue, snap frozen fresh and embedded in OCT, the antigenicity was retained after 6 years of storage. Our IHC staining was excellent. The frozen OCT/Tissue blocks were stored in a screw top 50 ml centrifuge tube. We avoid lower temperatures, and have a small under the counter freezer adjusted to -30C for storage of biochemicals, serum, and staining reagents. People have stored their OCT embedded tissues in this freezer, but not for 6 months. Personally, I have avoided this freezer for block storage mainly since we have a -80C freezer always availabe. It is important that the freezer is NOT a self defrosting freezer which I presume you are not using. Also, are you saying that the vial also contains isopentane and stored in this freezer? If so, you should not do this UNLESS the freezer is explosion proof or you will end up like the U of Arkansas lab that blew up and burned in the wee hours of the morning. Just store the frozen blocks in an airtight container, i.e. your tube with OCT + tissue, frozen in the tube. Bob Skinner wrote an article for HistoLogic (Sakura Finetek website) about the this accident and the dangers of storing isopentane in an improper freezer. There has been a great deal of discussion concerning frozen block storage on Histonet, so you may want to research it a bit more. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Cathy Malcontenti-Wilson" To: Sent: Sunday, April 20, 2008 6:20 PM Subject: [Histonet] Frozen sample storage Hi All, I would like some opinions on the storage of oct embedded tissues. These samples of animal tissues are embedded into 1 x 1cm pieces of oct and sit in a vial containing isopentane, and have been stored at -20 degrees for around 6 months. The freezer is only used for long term storage and so isn't opened very often (perhaps once per month). In the past I have stored my oct embedded tissues like this at -70 degrees. What would be your opinion of the antigenicity in these tissues if they were to be used for immunohistochemistry?? Thanks Cathy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Apr 21 09:29:32 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Apr 21 09:29:36 2008 Subject: [Histonet] immuno controls for cell blocks In-Reply-To: <3F328377AF4E23438E78234752652CE105D526D0@uwhis-xchng7.uwhis.hosp.wisc.edu> Message-ID: <526848.84039.qm@web65714.mail.ac4.yahoo.com> Jim: Your validation is adequate and you don't need to prepare a new set of (+) cell blocks. I think this CT will be smart enough to understand what a validation process is all about and that CAP is OK with that. It is always easy to ask, when you don't know what the answer involves! Ren? J. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Monday, April 21, 2008 8:27 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] immuno controls for cell blocks We are currently using a bank of formalin fixed tissue controls for our immunohistochemical stains. We have done a short study to show that the reactivity and specificity of our immunostains on the cellblocks, hardening in 95%ETOH and then transferred in formalin, is not different than our regularly formalin fixed tissue. As I read the new CAP checklist this is what we are required to do. Now a cytopathologist wants me to maintain a set of controls only for his cellblocks. How is everybody else handling this situation? Jim Vickroy Memorial Medical Center Springfield, Illinois. --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From anh2006 <@t> med.cornell.edu Mon Apr 21 10:10:58 2008 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Mon Apr 21 10:11:13 2008 Subject: [Histonet] Frozen sample storage In-Reply-To: <452655D3CC46464485FE5107C8723F2A01FB1C07@IS-EX-BEV1.unimelb.edu.au> References: <452655D3CC46464485FE5107C8723F2A01FB1C07@IS-EX-BEV1.unimelb.edu.au> Message-ID: I agree with the experts who have already replied that they should be fine ... but it's not 100% ideal. Unfortunately due to space we sometimes are forced to store blocks at -20 deg C too and they work just fine - as long as the cut surface is "sealed" and they are wrapped properly. I find at -20 deg C some people's blocks sort of shrivel up which is a disaster but I suspect this is because they weren't being stored wrapped in foil or in airtight containers and the cut surface was exposed. I would move to -80 deg C as soon as possible though. I store mine wrapped in foil in bitran bags and have reactivity for years. If for some reason the OCT seems gooey or dried out you can always cut away the old OCT and "reembed", if you will, it works well in a pinch. Note that if you were asking about cut sections it might be a whole different matter. I find cut sections don't always survive the warmer temp freezers so well. Antigenicity definitely gets lost over time. Good luck! >Hi All, > >I would like some opinions on the storage of oct embedded tissues. These >samples of animal tissues are embedded into 1 x 1cm pieces of oct and >sit in a vial containing isopentane, and have been stored at -20 degrees >for around 6 months. The freezer is only used for long term storage and >so isn't opened very often (perhaps once per month). In the past I have >stored my oct embedded tissues like this at -70 degrees. What would be >your opinion of the antigenicity in these tissues if they were to be >used for immunohistochemistry?? > > > >Thanks > >Cathy -- From b-frederick <@t> northwestern.edu Mon Apr 21 10:11:17 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon Apr 21 10:11:37 2008 Subject: [Histonet] molecular pathology In-Reply-To: <8CA71BB431D995A-FAC-3AD@Webmail-mg03.sim.aol.com> Message-ID: <000201c8a3c1$f36c5d40$d00f7ca5@lurie.northwestern.edu> We have 2 techs that do DNA and RNA extraction for tissue and blood. One is an MT and the other is experienced in cytogenetics and basic histo. The woman who does FISH is exteremely well versed in doing FISH and Western blot. She has been doing it forever (and can do it in her sleep). Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: Monday, April 21, 2008 6:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] molecular pathology How many histology labs are doing molecular pathology testing?? Like FISH, break aparts, PCR, etc. Who do you have performing the tests?? Are the Histotechs doing it, or have you hired a cytogenetics tech?? Or someone else? Roxanne _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Mon Apr 21 10:32:12 2008 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon Apr 21 10:32:17 2008 Subject: [Histonet] California Symposium Message-ID: Just a reminder that the California Society for Histotechnology is having their annual symposium in Ventura. The dates are Thursday, May 15 to Sunday May 18. There are some great workshops lined up. Also remember that if you were certified in 1994 and beyond you are required to complete continuing education. This is a great opportunity to get many of the required units. Please visit the CSH website for the program, or contact me. http://www.californiahistology.org/. Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu From sheila_adey <@t> hotmail.com Mon Apr 21 10:56:04 2008 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Mon Apr 21 10:56:12 2008 Subject: [Histonet] Choosing new microtome suggestions.... Message-ID: Good day netters, We are in the market for a new microtome. We have already demo'd the Microm and Leica. Any other suggestions? Thanks in advance _________________________________________________________________ If you like crossword puzzles, then you'll love Flexicon, a game which combines four overlapping crossword puzzles into one! http://g.msn.ca/ca55/208 From nancy_schmitt <@t> pa-ucl.com Mon Apr 21 10:59:31 2008 From: nancy_schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Mon Apr 21 10:59:49 2008 Subject: [Histonet] Hepatocyte Specific Antigen Message-ID: <9FC023A4AB52BB4D87DC6456081A822C087C6D@mercury.pa-ucl.com> Hello histonetters - Working up Hepatocyte marker on BOND stainer. Not really getting the results we wanted. Calls for no pretreatment - is anybody doing something different? Thanks for your help Nancy Schmitt MLT, HT (ASCP) Histology Coordinator Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From Beatrice.Debrosse-Serra <@t> pfizer.com Mon Apr 21 10:59:38 2008 From: Beatrice.Debrosse-Serra <@t> pfizer.com (Debrosse-Serra, Beatrice) Date: Mon Apr 21 10:59:53 2008 Subject: [Histonet] Choosing new microtome suggestions.... In-Reply-To: Message-ID: <8404DFBED5207B4B8E5EEF4332CEEA5306E67762@lajamrexm01.amer.pfizer.com> Either one is great, but my vote goes to the Leica 2255. Beatrice DeBrosse-Serra Pathology Scientist Pfizer Global Research & Development CB4, 2150 10646 Science Center Drive San Diego, CA 92121 Phone# 858-622-5986 Fax# 858-678-8290 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sheila adey Sent: Monday, April 21, 2008 8:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Choosing new microtome suggestions.... Good day netters, We are in the market for a new microtome. We have already demo'd the Microm and Leica. Any other suggestions? Thanks in advance _________________________________________________________________ If you like crossword puzzles, then you'll love Flexicon, a game which combines four overlapping crossword puzzles into one! http://g.msn.ca/ca55/208_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Mon Apr 21 11:09:39 2008 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Mon Apr 21 11:09:48 2008 Subject: [Histonet] Hepatocyte Specific Antigen In-Reply-To: <9FC023A4AB52BB4D87DC6456081A822C087C6D@mercury.pa-ucl.com> Message-ID: Nancy, We're using Biocare's HSA (CM 166), 1:50, on our Ventana instruments but do use HIER. Off-line we use a high pH Tris buffer for 2" in a lab pressure cooker and on-line we use Ventana's CC1 which is also a Tris buffer. We achieve beautiful, clean staining using these protocols. Maybe you can adapt this to your Bond stainer. Good luck, Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Monday, April 21, 2008 11:00 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Hepatocyte Specific Antigen Hello histonetters - Working up Hepatocyte marker on BOND stainer. Not really getting the results we wanted. Calls for no pretreatment - is anybody doing something different? Thanks for your help Nancy Schmitt MLT, HT (ASCP) Histology Coordinator Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Mon Apr 21 11:17:04 2008 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Mon Apr 21 11:17:25 2008 Subject: [Histonet] immuno controls for cell blocks In-Reply-To: <24A4826E8EF0964D86BC5317306F58A50680FF78BC@mmc-mail.ad.mhsil.com> References: <24A4826E8EF0964D86BC5317306F58A50680FF78BC@mmc-mail.ad.mhsil.com> Message-ID: <480C85C0020000770000C203@gwmail4.harthosp.org> We use our regular "formalin-fixed, paraffin-embedded" control tissues for all cytology IHC applications (direct smears, Thin-Preps, and cell blocks). Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Vickroy, Jim" 04/21/08 9:27 AM >>> We are currently using a bank of formalin fixed tissue controls for our immunohistochemical stains. We have done a short study to show that the reactivity and specificity of our immunostains on the cellblocks, hardening in 95%ETOH and then transferred in formalin, is not different than our regularly formalin fixed tissue. As I read the new CAP checklist this is what we are required to do. Now a cytopathologist wants me to maintain a set of controls only for his cellblocks. How is everybody else handling this situation? Jim Vickroy Memorial Medical Center Springfield, Illinois. This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From BMolinari <@t> heart.thi.tmc.edu Mon Apr 21 11:36:55 2008 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Mon Apr 21 11:37:04 2008 Subject: [Histonet] ORO Message-ID: Is it possible to do an ORO on gross samples? Thanks. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. MC 1-283 Houston, TX 77030 832-355-6524 832-355-6812 (fax) From MAUGER <@t> email.chop.edu Mon Apr 21 11:46:02 2008 From: MAUGER <@t> email.chop.edu (Joanne Mauger) Date: Mon Apr 21 11:46:42 2008 Subject: [Histonet] Hepatocyte Specific Antigen Message-ID: Nancy, Weuse HIER with citrate buffer, pH 6.0- should translate to the ER1 buffer on the Bond. Dilution we use with Envision+ is 1:100. I have diluted most antibodies out further with the Bond detection-try 1:200. Jo Mauger Children's Hospital of Philadelphia From BMolinari <@t> heart.thi.tmc.edu Mon Apr 21 11:54:18 2008 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Mon Apr 21 11:54:28 2008 Subject: [Histonet] ORO In-Reply-To: <8785CEF5DCC41A4F8014179C435935D602D378@exchange.unipathllc.corp> References: <8785CEF5DCC41A4F8014179C435935D602D378@exchange.unipathllc.corp> Message-ID: Do you have a protocol to share? -----Original Message----- From: Donella Stillings [mailto:DStillings@unipathllc.com] Sent: Monday, April 21, 2008 11:47 AM To: Molinari, Betsy Subject: RE: [Histonet] ORO Yes, Touch preps -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Monday, April 21, 2008 10:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ORO Is it possible to do an ORO on gross samples? Thanks. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. MC 1-283 Houston, TX 77030 832-355-6524 832-355-6812 (fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pmarcum <@t> vet.upenn.edu Mon Apr 21 12:22:28 2008 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Mon Apr 21 12:22:33 2008 Subject: [Histonet] Choosing new microtome suggestions.... In-Reply-To: References: Message-ID: <6.2.5.6.2.20080421132047.01caa450@vet.upenn.edu> We have two RM 2255 from Leica and love them. We also have the older RM2155 which is a very good microtome. The motors even hold up to our MMA blocks and tungsten carbide knives and then cut plastic just as well on disposable. Pam Marcum At 11:56 AM 4/21/2008, sheila adey wrote: >Good day netters, > >We are in the market for a new microtome. We have already demo'd the >Microm and Leica. Any other suggestions? > >Thanks in advance >_________________________________________________________________ >If you like crossword puzzles, then you'll love Flexicon, a game >which combines four overlapping crossword puzzles into one! >http://g.msn.ca/ca55/208_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From John.Spair <@t> multicare.org Mon Apr 21 12:26:06 2008 From: John.Spair <@t> multicare.org (John Spair) Date: Mon Apr 21 12:49:19 2008 Subject: [Histonet] RE: Histonet Digest, Vol 53, Issue 31 In-Reply-To: <6412152B2N016478-01@MMS_multicare.org> References: <6412152B2N016478-01@MMS_multicare.org> Message-ID: <61A9977919846C479389493BAE2517CA02274CCB@MHSEXMBX1.multicare.org> Liz: Richard Allan has product called Cyto Stain, that and hematoxylin nuclear stain is all you need for a cyto stain Date: Sun, 20 Apr 2008 12:52:09 -0600 From: "Liz Chlipala" Subject: [Histonet] need help with PAP stain To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hello everyone I need to run a pap stain on some saliva samples and I don't know where to begin. I know this seems like a silly question, but I have done some searching on the net and there are quite a few protocols with different reagents. Its not a large project for us, I only need to run about 30 to 40 slides and was hoping to purchase a kit that would contain the hematoxylin, OG-6 and EA-50 but the only one I can find is the one from thermo the rapid chrome pap staining kit but I can't seem to find a vendor that sells it. If possible I would like to purchase pints of these reagents rather than gallons. Any advice as to what reagents to purchase and a protocol would be helpful. Also advice on what the stain should look like would help too. Thanks in advance Liz "MMS " made the following annotations. ------------------------------------------------------------------------------ NOTICE: This e-mail and the attachments hereto, if any, may contain privileged and/or confidential information. It is intended only for use by the named addressee(s). If you are not the intended recipient of this e-mail, you are hereby notified that any examination, distribution or copying of this e-mail and the attachments hereto, if any, is strictly prohibited. If you have received this transmission in error, please immediately notify the sender by email or telephone and permanently delete this e-mail and the attachments hereto, if any, and destroy any printout thereof. MultiCare Health System, Tacoma, WA 98415 (253) 403-1000. ============================================================================== From CIngles <@t> uwhealth.org Mon Apr 21 14:13:50 2008 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Mon Apr 21 14:15:37 2008 Subject: [Histonet] ORO References: Message-ID: <08A0A863637F1349BBFD83A96B27A50A12011F@uwhis-xchng3.uwhis.hosp.wisc.edu> Betsy: We do them on frozens all the time. I don't know what doing a touch prep, etc. would show. Be interesting to see the results. Claire ________________________________ Is it possible to do an ORO on gross samples? Thanks. From fong <@t> zoology.ubc.ca Mon Apr 21 14:33:04 2008 From: fong <@t> zoology.ubc.ca (Angelina Fong) Date: Mon Apr 21 14:33:19 2008 Subject: [Histonet] Tract tracing from cell bodies to dendrites Message-ID: <480CEBF0.1030501@zoology.ubc.ca> Hi everyone, I am hoping some one will be able to help me and a friend in the lab ... here's the problem: We are wanting to find the afferent origin for a bundle of nerves in reptiles. We know the nerve have dendritic endings in the aorta and possibly pulmonary blood vessels in the thorax. The cell bodies of these nerves resides in a number of large ganglia in the neck and their axon project to the brainstem. However, we do not know the exact location of the dendritic ending in the blood vessels and would like to use tract tracing methods to find them. At this point, we don't need to clearly differentiate between afferents and efferents, although if that is possible it would be great! Most tract tracers refer to the direction of travel between axon terminals and cell bodies, so here are our questions: /Are there tract tracers that will travel towards the dendritic ends? /If so what would be the best tracers for this purpose? We have tried DiI but it is very slow, so some thing with more active transport would be appreciated. Will WGA or Dextran work for this? We have had very little luck finding out about this direction of travel. Or are there alternative ways we can identify connections between the nerves of interest and the blood vessels? Degeneration perhaps? Thank you so much for any ideas any one can give us!! Cheers Angelina - ~~o~~o~~o~~o~~o~~o~~o~~o~~o~~o Angelina Y. Fong, Ph.D. Department of Zoology Biological Sciences Building 6270 University Boulevard University of British Columbia Vancouver, BC, V6T 1Z4 Canada Ph: (604) 822-5990 Fax: (604) 822-2416 Email: fong@zoology.ubc.ca From mlgiebel <@t> vcu.edu Mon Apr 21 14:36:23 2008 From: mlgiebel <@t> vcu.edu (Mary L Giebel/FS/VCU) Date: Mon Apr 21 14:36:32 2008 Subject: [Histonet] Advise requested on paraffin processing problem Message-ID: Can anything be done to salvage tissue (murine heart) that has been damaged in overheated paraffin? The sections lack morphological detail and once they are dry, crack or else fall apart. Any suggestions would be appreciated. Thanks, Mary From rjbuesa <@t> yahoo.com Mon Apr 21 14:58:11 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Apr 21 14:58:14 2008 Subject: [Histonet] ORO In-Reply-To: Message-ID: <861870.1708.qm@web65708.mail.ac4.yahoo.com> Sure it can be done, but two things: 1- it will be expensive (too much reagent wasted), and 2- why would you want to do that? Ren? J. "Molinari, Betsy" wrote: Is it possible to do an ORO on gross samples? Thanks. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. MC 1-283 Houston, TX 77030 832-355-6524 832-355-6812 (fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From akemiat3377 <@t> yahoo.com Mon Apr 21 15:24:18 2008 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Mon Apr 21 15:24:21 2008 Subject: [Histonet] molecular pathology In-Reply-To: <8CA71BB431D995A-FAC-3AD@Webmail-mg03.sim.aol.com> Message-ID: <768776.10746.qm@web31308.mail.mud.yahoo.com> Here at PhenoPath Laboratories, we do FISH, ISH, PCR and a 9-Color Flow evaluation. We have Med Tech's, as well as histotech's that perform the Fish, ISH and Flow studies. Our PCR's are currently being performed by a Ph.d. and a histotech that is obtaining a specialized molecular certificate. Akemi Allison-Tacha Client Services Manager PhenoPath Laboratories 551 N 34th St., #100 Seattle, WA 98103 Work #: (206) 374-9000 ext 1053 E-Mail: akemi@phenopath.com Website: http://www.phenopath.com --- godsgalnow@aol.com wrote: > How many histology labs are doing molecular > pathology testing?? Like FISH, break aparts, PCR, > etc. > > Who do you have performing the tests?? Are the > Histotechs doing it, or have you hired a > cytogenetics tech?? Or someone else? > > Roxanne > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Akemi Allison-Tacha, BS, HT(ASCP)HTL Client Services Manager PhenoPath laboratories 551 North 34th Street, Suite 100 Seattle, WA 98103-8675 Work: (206) 374-9000 ext 1053 E-Mail: akemiat3377@yahoo.com From gayle.callis <@t> bresnan.net Mon Apr 21 15:43:02 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Mon Apr 21 15:43:10 2008 Subject: [Histonet] ORO References: <861870.1708.qm@web65708.mail.ac4.yahoo.com> Message-ID: <000d01c8a3f0$4b5fb0a0$6501a8c0@DHXTS541> The Churukian method is not expensive compared to the classic method using propylene glycol (expensive and messy). A grossly thick cross section of arterial plaque would not be an unreasonable sample to stain with ORO. If anyone is interested in the Churukian method, published in JOH a few years back, I will be happy to attach privately. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Rene J Buesa" To: "Molinari, Betsy" ; Sent: Monday, April 21, 2008 1:58 PM Subject: Re: [Histonet] ORO > Sure it can be done, but two things: > 1- it will be expensive (too much reagent wasted), and > 2- why would you want to do that? > Ren? J. > > "Molinari, Betsy" wrote: > Is it possible to do an ORO on gross samples? > > Thanks. > > > > Betsy Molinari HT(ASCP) > > Texas Heart Institute > > Cardiovascular Pathology > > 6770 Bertner Ave. > > MC 1-283 > > Houston, TX 77030 > > 832-355-6524 > > 832-355-6812 (fax) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it > now. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hborgeri <@t> wfubmc.edu Mon Apr 21 16:12:31 2008 From: hborgeri <@t> wfubmc.edu (Hermina Borgerink) Date: Mon Apr 21 16:12:36 2008 Subject: [Histonet] ORO In-Reply-To: <000d01c8a3f0$4b5fb0a0$6501a8c0@DHXTS541> References: <861870.1708.qm@web65708.mail.ac4.yahoo.com> <000d01c8a3f0$4b5fb0a0$6501a8c0@DHXTS541> Message-ID: <9AEEF1FB6254224AA355ED285F8491652B76DAFC@EXCHVS2.medctr.ad.wfubmc.edu> Nearly 25 years ago, we routinely stained gross monkey aortas using Sudan IV, and obtained beautiful results. The only drawback, a spectrophotometer is required to make up the stain. I'll be glad to share the protocol in private as it is rather lengthy. Hermina Hermina M. Borgerink, BA, HT, HTL(ASCP)QIHC Wake Forest University Primate Center Department of Pathology Medical Center Blvd. Winston-Salem, NC 27157 Tel. (336) 716-1538 Fax. (336) 716-1515 e-mail: hborgeri@wfubmc.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Monday, April 21, 2008 4:43 PM To: Rene J Buesa; Molinari, Betsy; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] ORO The Churukian method is not expensive compared to the classic method using propylene glycol (expensive and messy). A grossly thick cross section of arterial plaque would not be an unreasonable sample to stain with ORO. If anyone is interested in the Churukian method, published in JOH a few years back, I will be happy to attach privately. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Rene J Buesa" To: "Molinari, Betsy" ; Sent: Monday, April 21, 2008 1:58 PM Subject: Re: [Histonet] ORO > Sure it can be done, but two things: > 1- it will be expensive (too much reagent wasted), and > 2- why would you want to do that? > Ren? J. > > "Molinari, Betsy" wrote: > Is it possible to do an ORO on gross samples? > > Thanks. > > > > Betsy Molinari HT(ASCP) > > Texas Heart Institute > > Cardiovascular Pathology > > 6770 Bertner Ave. > > MC 1-283 > > Houston, TX 77030 > > 832-355-6524 > > 832-355-6812 (fax) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Be a better friend, newshound, and know-it-all with Yahoo! Mobile. > Try it now. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Apr 21 16:17:26 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Apr 21 16:17:29 2008 Subject: [Histonet] Advise requested on paraffin processing problem In-Reply-To: Message-ID: <270576.93417.qm@web65715.mail.ac4.yahoo.com> I am afraid that this type of damage is irreversible. Ren? J. Mary L Giebel/FS/VCU wrote: Can anything be done to salvage tissue (murine heart) that has been damaged in overheated paraffin? The sections lack morphological detail and once they are dry, crack or else fall apart. Any suggestions would be appreciated. Thanks, Mary _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From mariakatleba <@t> aol.com Mon Apr 21 17:10:28 2008 From: mariakatleba <@t> aol.com (mariakatleba@aol.com) Date: Mon Apr 21 17:10:43 2008 Subject: [Histonet] Dissect-Aid vs 95%Alcohol Message-ID: <8CA7213A42DB076-FF0-1D92@webmail-nb12.sysops.aol.com> Hi All, I have a dilemma. I have a lab assistant who does not use the dissect aid to find lymph nodes. She will not wear a respirator so she prefers 95% alcohol. From many years experience, I have seen that the 95% alcohol ruins morphology but also destroys the tissue for future Immuno stains. Can anyone find some documentation to support my claim that we SHOULD use DissectAid and NOT alcohol???? Kind regards Maria Katleba MS HT Pathology Dept Mgr 707-257-4076 QVMC Napa CA 94558 From wattenho <@t> titus.u-strasbg.fr Tue Apr 22 04:33:42 2008 From: wattenho <@t> titus.u-strasbg.fr (Marie Wattenhofer) Date: Tue Apr 22 04:33:56 2008 Subject: [Histonet] Modified Gomori Trichrome Message-ID: <1831.130.79.78.169.1208856822.squirrel@130.79.78.169> Hi I am a beginner in histology.... I would like to do the modified gomori trichrome staining to see if I have RRF on muscle frozen sections. I tried the following protocol that I found on the web Preparation of solutions: SolutionA: Chromotrope 2R 60 mg FCF 30 mg phosphotunstic acid 60 mg glacial acid acetic 1 ml Water up to 100ml Adjust pH to 3.4 with HCL 37% Solution B 200 ul of glacial acetic acid in 100 ml of water Protocole Frozen muscle section 3 mn in filtred Hematoxiline Harris Wash 3 times in water 10 mn in Solution A Wash 3 times in water 10 seconds in solution B Deshydratation: EtOH 70%, 95% absolute Histosol 10 mn I got the following result. >From the border to the center of the cut the coloration was not the same: At the border of the cut, fibers are green (great!) but then they are red (???) and at the very center, they are not colored at all. Could someone tell me what I did wrong? should I stain longer in solution A ? I have read that the pH of solution A is VERY important; was it ok to pH with HCl? Is the solution B formula correct? Thanks for your help! Marie -- Marie WATTENHOFER-DONZE Institut de Genetique et de Biologie Moleculaire et Cellulaire (IGBMC) 1 rue Laurent Fries, BP 10142 67404 Illkirch Cedex France Tel. 33+(0)3 88 65 34 16 web site : http://www-igbmc.u-strasbg.fr From pieronelva01 <@t> bigpond.com Tue Apr 22 04:44:53 2008 From: pieronelva01 <@t> bigpond.com (Piero Nelva) Date: Tue Apr 22 04:45:08 2008 Subject: [Histonet] immuno controls for cell blocks References: <3F328377AF4E23438E78234752652CE105D526D0@uwhis-xchng7.uwhis.hosp.wisc.edu> Message-ID: <001a01c8a45d$844a3a30$4475be7c@pentium4> I agree. It's hard enough keeping a store of IHC control material anyway!! Piero Nelva Anatomical PAthology Monash Medical Centre Australia ----- Original Message ----- From: "Drew Sally A." To: "Histonet" Sent: Monday, April 21, 2008 11:40 PM Subject: RE: [Histonet] immuno controls for cell blocks We are only using routinely processed FFPE tissues as controls for cell blocks. Depending on what antibodies you might run on cell blocks, I would think it difficult and impractical to try to obtain enough material to create cell block controls... Sally Ann Drew, MT(ASCP) IHC/ISH Laboratory University of Wisconsin Hosp. & Clinics Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Monday, April 21, 2008 8:27 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] immuno controls for cell blocks We are currently using a bank of formalin fixed tissue controls for our immunohistochemical stains. We have done a short study to show that the reactivity and specificity of our immunostains on the cellblocks, hardening in 95%ETOH and then transferred in formalin, is not different than our regularly formalin fixed tissue. As I read the new CAP checklist this is what we are required to do. Now a cytopathologist wants me to maintain a set of controls only for his cellblocks. How is everybody else handling this situation? Jim Vickroy Memorial Medical Center Springfield, Illinois. This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG. Version: 7.5.524 / Virus Database: 269.23.3/1390 - Release Date: 4/21/2008 4:23 PM From pruegg <@t> ihctech.net Tue Apr 22 07:24:09 2008 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Tue Apr 22 07:16:35 2008 Subject: [Histonet] kyamamoto IHCRG Membership Application Form Message-ID: <200804221216.m3MCGJbS013976@pro42.abac.com> Verify NSH membership please _____ From: webmaster@neo.agsci.colostate.edu [mailto:webmaster@neo.agsci.colostate.edu] Sent: Monday, April 21, 2008 2:05 PM To: patsy.ruegg@gmail.com Subject: IHCRG Membership Application Form _____ New_Application: Yes Last_Name: yamamoto First_Name: karen NSH_Member: Yes Employer: carl zeiss microimaging Address: 31 columbia, suite 101 City: aliso viejo State: CA Zip: 92656 Province: Country: usa Phone: (949) 448-3100 Ext: Fax: (949) 448-9053 Email: kyamamoto@zeiss.com Surgical_Pathology: Hematopathology: Yes Orthopedic: Veterinary: Immunology: Yes Plastics: Research: Paraffin: Yes Frozen: Yes Cytospins: Yes Smears_Touch_Preps: Yes Plastics_Sample: Sample_Other: PAP: Yes APAAP: Yes ABC_HRP: Yes ABC_AP: SA_HRP: Yes SA_AP: IF: IHC_Other: ISH: Yes Gels: PCR: Mol_Other: Automated_IHC: Yes Company: Dako IHC_Qualification: No Date_Taken_Exam: Like_To_Take_Exam: No Expected_Date: Can_Provide_Tissues: No Tissues_Provided: Need_Tissues: No Tissue_Needed: Remote Name: 38.102.244.2 HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1) Date: 04/21/2008 Time: 02:05 PM Other_Tech From kenneth.metzger <@t> aruplab.com Tue Apr 22 09:30:54 2008 From: kenneth.metzger <@t> aruplab.com (Metzger, Kenneth) Date: Tue Apr 22 09:30:32 2008 Subject: [Histonet] Help Message-ID: Is anyone or does anyone know a good EM contact whose brain I could pick? Thanks, Ken Ken Metzger HTL(ASCP) Histology Supervisor ARUP Laboratories 500 Chipeta way Salt Lake City, UT 84108 801.583.2787 ext 3101 - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 From JMahoney <@t> alegent.org Tue Apr 22 09:43:47 2008 From: JMahoney <@t> alegent.org (Mahoney,Janice A) Date: Tue Apr 22 09:44:39 2008 Subject: [Histonet] unsubscribe Message-ID: <346E5878979BA54FB4B0BFD6AD93B9B9B01EE9626E@EXCHMBC1.ad.ah.local> ________________________________ Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From pruegg <@t> ihctech.net Tue Apr 22 10:48:05 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Apr 22 10:48:17 2008 Subject: [Histonet] pardon me Message-ID: <002801c8a490$43005400$6401a8c0@Patsyoffice> Dear Histonet users, I accidentally sent a message to histonet that was meant to go to histo@nsh.org asking for NSH membership verification for someone joining the IHC Resource Group, please ignore that message it was a mistake. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From leskovjan <@t> bnl.gov Tue Apr 22 11:13:17 2008 From: leskovjan <@t> bnl.gov (Leskovjan, Andreana) Date: Tue Apr 22 11:13:31 2008 Subject: [Histonet] Thioflavin S staining Message-ID: <07C6074DBBED004F9079CD1FC74FAEA7F01570@exchangemb1.bnl.gov> Hi, I need to stain 30 um frozen, unfixed mouse brain sections with Thioflavin S. The sections are mounted onto a very thin plastic film called Ultralene. I know it's not the best but necessary for our measurements. I have been using a very simple procedure so far with minimal chemicals to avoid interference with the measurements: 50% ethanol for 5 min; 0.0125% Thioflavin S for 3 min; wash in 50% ethanol, then nanopure water. The staining works however the tissue is almost destroyed. There are holes, tears, and folds everywhere, which makes the plaques difficult to see and measure. I've tried many combinations of the above and this is the best I could get. Does anyone have a suggestion on how to prevent this and get better looking sections? Thanks, Andreana Leskovjan From TJJ <@t> Stowers-Institute.org Tue Apr 22 12:14:13 2008 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Tue Apr 22 12:14:38 2008 Subject: [Histonet] Re: Modified Gomori Trichrome Message-ID: Marie, I have no direct experience with doing trichrome stains on frozen muscle, but a quick histonet search turned up this wonderful post by Peggy Wenk: http://www.histosearch.com/histonet/Jul06A/RE.HistonetHowtostainfrozA.html Additionally she provides this great reference: http://www.neuro.wustl.edu/neuromuscular/ Good luck to you! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From crochieresteve <@t> aol.com Tue Apr 22 12:23:16 2008 From: crochieresteve <@t> aol.com (crochieresteve@aol.com) Date: Tue Apr 22 12:23:29 2008 Subject: [Histonet] MIcrotome suggestions Message-ID: <8CA72B4AFB2DF32-1618-A4C@webmail-da04.sysops.aol.com> For FFPE I like the Thermo Finesse microtome. sc From Dorothy.L.Webb <@t> HealthPartners.Com Tue Apr 22 12:35:35 2008 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Tue Apr 22 12:35:42 2008 Subject: [Histonet] Marking inks Message-ID: <0E394B648E5284478A6CCB78E5AFDA2705635658@hpes1.HealthPartners.int> Has anyone ever experienced any of the colors or brands of marking inks drying out the needle biopsies? We are experiencing prostate needle bx's that, at times, are dryer than other processing times and a tech questioned me if possibly the marking ink could be contributing to this problem. Any thoughts, please!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From mpence <@t> grhs.net Tue Apr 22 12:41:27 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Apr 22 12:41:42 2008 Subject: [Histonet] Marking inks In-Reply-To: <0E394B648E5284478A6CCB78E5AFDA2705635658@hpes1.HealthPartners.int> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A37EC@IS-E2K3.grhs.net> Why are you putting marking inks on prostate bx's. I just have to ask! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Tuesday, April 22, 2008 12:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Marking inks Has anyone ever experienced any of the colors or brands of marking inks drying out the needle biopsies? We are experiencing prostate needle bx's that, at times, are dryer than other processing times and a tech questioned me if possibly the marking ink could be contributing to this problem. Any thoughts, please!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Tue Apr 22 13:58:06 2008 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue Apr 22 12:59:36 2008 Subject: SPAM-LOW: RE: [Histonet] Marking inks In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A37EC@IS-E2K3.grhs.net> Message-ID: Mike, This is done to identify the biopsies location/site (by different color of ink) when multiple pieces are placed in one cassette. This is done instead of placing each biopsy in a separate cassette (I.E. First cassette would be A and B, second cassette would be C and D, and so on). Instead of embedding and cutting 16 cassettes, you would be embedding and cutting 8. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Tuesday, April 22, 2008 12:41 PM To: Webb, Dorothy L; histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: RE: [Histonet] Marking inks Why are you putting marking inks on prostate bx's. I just have to ask! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Tuesday, April 22, 2008 12:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Marking inks Has anyone ever experienced any of the colors or brands of marking inks drying out the needle biopsies? We are experiencing prostate needle bx's that, at times, are dryer than other processing times and a tech questioned me if possibly the marking ink could be contributing to this problem. Any thoughts, please!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Tue Apr 22 13:47:23 2008 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Tue Apr 22 13:47:27 2008 Subject: [Histonet] Thioflavin S staining In-Reply-To: <07C6074DBBED004F9079CD1FC74FAEA7F01570@exchangemb1.bnl.gov> References: <07C6074DBBED004F9079CD1FC74FAEA7F01570@exchangemb1.bnl.gov> Message-ID: Your description gives the impression of damage caused by slow freezing tissue, leaving beh buffered formaldehyde, in 30% aqueous sucrose fo sinks). Even with cryoprotection, as quickly as possible. There have been this subject over the years. They can be fou href="http://www.histosearch.com/">www.histos earch.com.
 
John Kiernan
Anat = =
----- Or "Leskovjan, Andreana" < leskovjan@bnl.gov>
Date: Tuesday, April 22, 2008 staining
T histonet@lists.utsouthwestern.edu

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>
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> _______ _______________________ 5F Histone Histonet@lists.utsouthwestern.edu< http://lists.utsouthwestern.edu/mailman/listinfo/his From mward <@t> wfubmc.edu Tue Apr 22 13:51:56 2008 From: mward <@t> wfubmc.edu (Martha Ward) Date: Tue Apr 22 13:52:37 2008 Subject: [Histonet] IGF-IR antibody Message-ID: <61135F0455D33347B5AAE209B903A30422968C9C@EXCHVS2.medctr.ad.wfubmc.edu> Hello all, I have had a researcher approach me about working up this antibody. Does anyone have any experience with it, especially on the Bond, and could you suggest a vendor? Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center Medical Center Blvd. Winston-Salem, NC 27157 336-716-2756 mward@wfubmc.edu From jmjohnson34 <@t> hotmail.com Tue Apr 22 13:53:23 2008 From: jmjohnson34 <@t> hotmail.com (Jennifer Johnson) Date: Tue Apr 22 13:53:30 2008 Subject: [Histonet] Bielschowsky Message-ID: My Pathologist has ordered a Bielschowsky stain for Alzheimer's and wants it performed today! I have everything except the concentrated Nitric Acid (1 drop). Do any of you know if this is absolutely necessary for the Developer Solution or if there is any other acid that I may substitute one drop of? Please reply off the list so I can start ASAP because I receive this in batch form. Thank you so much! Jennifer Johnson, HTL (ASCP) _________________________________________________________________ Express yourself wherever you are. Mobilize! http://www.gowindowslive.com/Mobile/Landing/Messenger/Default.aspx?Locale=en-US?ocid=TAG_APRIL From anh2006 <@t> med.cornell.edu Tue Apr 22 14:04:24 2008 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Tue Apr 22 14:04:27 2008 Subject: [Histonet] IGF-IR antibody In-Reply-To: <61135F0455D33347B5AAE209B903A30422968C9C@EXCHVS2.medctr.ad.wfubmc.edu> References: <61135F0455D33347B5AAE209B903A30422968C9C@EXCHVS2.medctr.ad.wfubmc.edu> Message-ID: From my records of work I did several years go I say try the one from Lab Vision Neomarkers. Anti-IGF1R, clone 24-31, Cat # MS-641-P. It worked well for me on FFPE sections. I see Cell Signaling has one that looks like it works beautifully as well. >Hello all, > >I have had a researcher approach me about working up this antibody. >Does anyone have any experience with it, especially on the Bond, and >could you suggest a vendor? > >Thanks in advance for your help. > >Martha Ward, MT (ASCP) QIHC >Assistant Manager, Molecular Diagnostics Lab >Wake Forest University Baptist Medical Center >Medical Center Blvd. >Winston-Salem, NC 27157 >336-716-2756 >mward@wfubmc.edu > -- From leskovjan <@t> bnl.gov Tue Apr 22 14:10:01 2008 From: leskovjan <@t> bnl.gov (Leskovjan, Andreana) Date: Tue Apr 22 14:10:20 2008 Subject: [Histonet] Thioflavin S staining In-Reply-To: Message-ID: <07C6074DBBED004F9079CD1FC74FAEA7F0158C@exchangemb1.bnl.gov> Hi John, Thank you very much for your reply. Unfortunately, the experimental design does not allow fixation nor cryoprotection. Also, the tissues look very good before staining and also when staining on regular glass slides. Do you have any advice on keeping the tissues intact during staining on substrates other than glass? Thanks, Andreana ________________________________ From: John Kiernan [mailto:jkiernan@uwo.ca] Sent: Tuesday, April 22, 2008 2:47 PM To: Leskovjan, Andreana Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Thioflavin S staining Your description gives the impression of damage caused by slow freezing without cryoprotection. Ice crystals form in the tissue, leaving behind holes. Prevention: adequate fixation in buffered formaldehyde, followed by cryoprotection (usually soaking in 30% aqueous sucrose for a day or two; until the brain sinks). Even with cryoprotection, the specimen should be frozen as quickly as possible. There have been many Histonet postings on this subject over the years. They can be found at www.histosearch.com . John Kiernan Anatomy, UWO London, Canada. = = = ----- Original Message ----- From: "Leskovjan, Andreana" Date: Tuesday, April 22, 2008 12:16 Subject: [Histonet] Thioflavin S staining To: histonet@lists.utsouthwestern.edu > Hi, > > > > I need to stain 30 um frozen, unfixed mouse brain sections with > Thioflavin S. The sections are mounted onto a very thin > plastic film > called Ultralene. I know it's not the best but necessary > for our > measurements. I have been using a very simple procedure so > far with > minimal chemicals to avoid interference with the > measurements: 50% > ethanol for 5 min; 0.0125% Thioflavin S for 3 min; wash in 50% > ethanol,then nanopure water. The staining works however > the tissue is almost > destroyed. There are holes, tears, and folds everywhere, > which makes > the plaques difficult to see and measure. I've tried many > combinationsof the above and this is the best I could get. > Does anyone have a > suggestion on how to prevent this and get better looking > sections? > > > > Thanks, > > Andreana Leskovjan > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Apr 22 14:13:31 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Apr 22 14:13:35 2008 Subject: [Histonet] Thioflavin S staining In-Reply-To: <07C6074DBBED004F9079CD1FC74FAEA7F01570@exchangemb1.bnl.gov> Message-ID: <523134.27671.qm@web65702.mail.ac4.yahoo.com> If the sections HAVE to be unfixed, you are doing the best you can. IF they can be fixed (NBF for instance) then their integrity will be assured and the staining could be the same. Ren? J. "Leskovjan, Andreana" wrote: Hi, I need to stain 30 um frozen, unfixed mouse brain sections with Thioflavin S. The sections are mounted onto a very thin plastic film called Ultralene. I know it's not the best but necessary for our measurements. I have been using a very simple procedure so far with minimal chemicals to avoid interference with the measurements: 50% ethanol for 5 min; 0.0125% Thioflavin S for 3 min; wash in 50% ethanol, then nanopure water. The staining works however the tissue is almost destroyed. There are holes, tears, and folds everywhere, which makes the plaques difficult to see and measure. I've tried many combinations of the above and this is the best I could get. Does anyone have a suggestion on how to prevent this and get better looking sections? Thanks, Andreana Leskovjan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From rahayes <@t> serha.ca Tue Apr 22 14:21:58 2008 From: rahayes <@t> serha.ca (Hayes, Randi (R1SE)) Date: Tue Apr 22 14:22:06 2008 Subject: [Histonet] Recommended min/max fixation time - Bouin's for bone marrow Message-ID: Hi Histonetters, Just wondering if there are some recommendations when it comes to min and max times for bone marrows to fix in Bouin's? Thanks, Randi Hayes South East Regional Health Authority Moncton, NB randi.hayes@serha.ca ----- SERHA Disclaimer ----- This electronic transmission and any accompanying attachments may contain privileged or confidential information intended only for the use of the individual or organization named above. Any distribution, copying or action taken in reliance on the contents of this communication by anyone other than the intended recipient(s) is STRICTLY PROHIBITED. If you have received this communication in error please notify the sender at the above e-mail address and delete this e-mail immediately. Thank you. Cette communication ?lectronique et toute pi?ce jointe peuvent contenir de l'information de nature privil?gi?e ou confidentielle et sont strictement r?serv?es ? l'usage du destinataire vis? et identifi? ci-dessus. Si vous n'?tes pas le destinataire vis?, prenez avis que toute distribution, reproduction ou mesure fond?e sur l'information qui y est contenue est EXPRESS?MENT INTERDITE. Si vous avez re?u cette communication par erreur, veuillez en aviser imm?diatement l'exp?diteur par courriel (? l'adresse ?lectronique mentionn?e ci-dessus) et supprimer le message d'origine. Merci. From rjbuesa <@t> yahoo.com Tue Apr 22 14:46:02 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Apr 22 14:46:06 2008 Subject: [Histonet] Marking inks In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A37EC@IS-E2K3.grhs.net> Message-ID: <169964.17823.qm@web65715.mail.ac4.yahoo.com> I would like to know also, please! Needle biopsies do not require inking, UNLESS you want to know the original site (R, L, etc) BUT usually the come all together, so........? Ren? J. Mike Pence wrote: Why are you putting marking inks on prostate bx's. I just have to ask! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Tuesday, April 22, 2008 12:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Marking inks Has anyone ever experienced any of the colors or brands of marking inks drying out the needle biopsies? We are experiencing prostate needle bx's that, at times, are dryer than other processing times and a tech questioned me if possibly the marking ink could be contributing to this problem. Any thoughts, please!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From Heather.D.Renko <@t> osfhealthcare.org Tue Apr 22 14:50:22 2008 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Tue Apr 22 14:50:39 2008 Subject: [Histonet] RE: microtomes Message-ID: <40026EDDE64CDA47AB382C52619ACD3C0A09DEAD@pmc-rfd-mx01.intranet.osfnet.org> We have four different Leica models and really do like them along with the field service for upkeep of them. Heather D. Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 Direct: 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From ryaskovich <@t> dir.nidcr.nih.gov Tue Apr 22 16:01:56 2008 From: ryaskovich <@t> dir.nidcr.nih.gov (Yaskovich, Ruth A (NIH/NIDCR) [E]) Date: Tue Apr 22 16:02:06 2008 Subject: [Histonet] Cornea nerve fibers Message-ID: Hi all, does any one have a stain for nerve fibers in the cornea? Ruth Yaskovich National Institutes of Health From marktarango <@t> gmail.com Tue Apr 22 19:17:23 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Tue Apr 22 19:17:30 2008 Subject: [Histonet] Marking inks In-Reply-To: <169964.17823.qm@web65715.mail.ac4.yahoo.com> References: <661949901A768E4F9CC16D8AF8F2838C017A37EC@IS-E2K3.grhs.net> <169964.17823.qm@web65715.mail.ac4.yahoo.com> Message-ID: <5b6eb13e0804221717p75b1ad74u362ad2a9dd6e38d9@mail.gmail.com> I've seen labs do this so they can see the tissue while embedding. On Tue, Apr 22, 2008 at 12:46 PM, Rene J Buesa wrote: > I would like to know also, please! Needle biopsies do not require inking, > UNLESS you want to know the original site (R, L, etc) BUT usually the come > all together, so........? > Ren? J. > > Mike Pence wrote: > Why are you putting marking inks on prostate bx's. I just have to ask! > Mike > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, > Dorothy L > Sent: Tuesday, April 22, 2008 12:36 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Marking inks > > > Has anyone ever experienced any of the colors or brands of marking inks > drying out the needle biopsies? We are experiencing prostate needle > bx's that, at times, are dryer than other processing times and a tech > questioned me if possibly the marking ink could be contributing to this > problem. Any thoughts, please!! > > Dorothy Webb, HT (ASCP) > Histology Technical Supervisor > Regions Hospital, Pathology Department > 640 Jackson Street, Saint Paul, MN 55101-2595 > Phone: 651-254-2962 > Fax: 651-254-2741 > Regions Hospital is part of the HealthPartners family of care > ________________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they are > addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, please > be advised that you have received this e-mail in error and that any use, > dissemination, forwarding, printing, or copying of this e-mail is > strictly prohibited. > > If you have received this e-mail in error, please immediately notify the > HealthPartners Support Center by telephone at (952) 967-6600. You will > be reimbursed for reasonable costs incurred in notifying us. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it > now. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Wed Apr 23 07:05:50 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Apr 23 07:05:54 2008 Subject: [Histonet] Bielschowsky In-Reply-To: Message-ID: <68474.90519.qm@web65707.mail.ac4.yahoo.com> Try concentrated hydrochloric. Ren? J. Jennifer Johnson wrote: My Pathologist has ordered a Bielschowsky stain for Alzheimer's and wants it performed today! I have everything except the concentrated Nitric Acid (1 drop). Do any of you know if this is absolutely necessary for the Developer Solution or if there is any other acid that I may substitute one drop of? Please reply off the list so I can start ASAP because I receive this in batch form. Thank you so much! Jennifer Johnson, HTL (ASCP) _________________________________________________________________ Express yourself wherever you are. Mobilize! http://www.gowindowslive.com/Mobile/Landing/Messenger/Default.aspx?Locale=en-US?ocid=TAG_APRIL_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From rjbuesa <@t> yahoo.com Wed Apr 23 07:08:06 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Apr 23 07:08:14 2008 Subject: [Histonet] Cornea nerve fibers In-Reply-To: Message-ID: <276179.70423.qm@web65704.mail.ac4.yahoo.com> Try Bodian's Ren? J. "Yaskovich, Ruth A (NIH/NIDCR) [E]" wrote: Hi all, does any one have a stain for nerve fibers in the cornea? Ruth Yaskovich National Institutes of Health _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From acp566 <@t> gmail.com Wed Apr 23 07:55:50 2008 From: acp566 <@t> gmail.com (Aaron Parks) Date: Wed Apr 23 07:55:55 2008 Subject: [Histonet] IMPAC PowerPath In-Reply-To: <2b34fb980804230550o4f31224fo2f2bc1ae904f3995@mail.gmail.com> References: <2b34fb980804230550o4f31224fo2f2bc1ae904f3995@mail.gmail.com> Message-ID: <2b34fb980804230555u44e9454ciea764ba43918a13@mail.gmail.com> Hello, I am a histotech up here in Maine and we are looking to switch from our outdated custom Pathology LIS to IMPAC PowerPath. It seems to be a great system and before we brought them in to do a demo, has anyone used this system? I know they do not have a huge customer base, but it never hurts to ask... Thanks in advance, Aaron Parks HT(ASCP) DRH Systems Coordinator From victor <@t> pathology.washington.edu Wed Apr 23 09:35:55 2008 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Wed Apr 23 09:36:04 2008 Subject: [Histonet] IMPAC PowerPath In-Reply-To: <2b34fb980804230555u44e9454ciea764ba43918a13@mail.gmail.com> References: <2b34fb980804230550o4f31224fo2f2bc1ae904f3995@mail.gmail.com> <2b34fb980804230555u44e9454ciea764ba43918a13@mail.gmail.com> Message-ID: <480F494B.2020405@pathology.washington.edu> Aaron, They probably have around 400 + clients, MD Anderson, UCLA, Cedar Sinai and ourselves are just a sampling. We have been using the program since 1999 and have many custom modifications to make it better for our needs. Feel free to contact me with any questions. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Aaron Parks wrote: > Hello, > > I am a histotech up here in Maine and we are looking to switch from our > outdated custom Pathology LIS to IMPAC PowerPath. It seems to be a great > system and before we brought them in to do a demo, has anyone used this > system? I know they do not have a huge customer base, but it never hurts to > ask... > > Thanks in advance, > > Aaron Parks HT(ASCP) > DRH Systems Coordinator > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From victor <@t> pathology.washington.edu Wed Apr 23 12:19:54 2008 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Wed Apr 23 12:20:01 2008 Subject: [Histonet] Automated Special Stainers Message-ID: <480F6FBA.1000303@pathology.washington.edu> Our PA who oversees Grossing and Histology asked me to post a question to the group. We currently have the Artisan (about 5 years d) and would like to know if there is something better for quality and output. Thanks Victor -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From contact <@t> excaliburpathology.com Wed Apr 23 12:21:41 2008 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Wed Apr 23 12:21:50 2008 Subject: [Histonet] Cornea nerve fibers Message-ID: <996977.16027.qm@web50104.mail.re2.yahoo.com> ?The cornea?only has unmyelinated?nerve fibers, so a luxol fast blue will not work. Try Rene's suggestion. ?Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 631 N. Broadway Ave. Moore, OK 73160 405-759-3953 lab contact@excaliburpathology.com www.excaliburpathology.com From Ronald.Houston <@t> nationwidechildrens.org Wed Apr 23 12:24:29 2008 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Wed Apr 23 12:25:11 2008 Subject: [Histonet] Automated Special Stainers In-Reply-To: <480F6FBA.1000303@pathology.washington.edu> Message-ID: <979FF5962E234F45B06CF0DB7C1AABB20FEC1F46@chi2k3ms01.columbuschildrens.net> Nothing comes close as far as quality is concerned Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5450 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Wednesday, April 23, 2008 1:20 PM To: Histonet Subject: [Histonet] Automated Special Stainers Our PA who oversees Grossing and Histology asked me to post a question to the group. We currently have the Artisan (about 5 years d) and would like to know if there is something better for quality and output. Thanks Victor -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From POWELL_SA <@t> Mercer.edu Wed Apr 23 12:21:43 2008 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Wed Apr 23 12:26:40 2008 Subject: [Histonet] Free workshop Message-ID: <01MTYRHM02P2002IFU@Macon2.Mercer.edu> Hi Guys, Here is a free workshop with free CEUs for those in the Southeast who can get to Atlanta on May 21st. For more information go to our web site, GSH at www.hiistosearch.com/gsh, click on the education page or click on this link. http://www.histosearch.com/gsh/LeicaSymposium.pdf Shirley Powell GSH Secretary From jm.lapointe <@t> accellab.com Wed Apr 23 13:19:39 2008 From: jm.lapointe <@t> accellab.com (Jean-Martin Lapointe) Date: Wed Apr 23 13:22:02 2008 Subject: [Histonet] IGF-IR antibody (Martha Ward) Message-ID: I have no recent experience with IGF-1R IHC, but for such markers of cell function, in the past Cell Signaling has always been my first choice of company - one of the few companies that actually seemed to care about selling antibodies that work. __________________________________ Jean-Martin Lapointe From jqb7 <@t> cdc.gov Wed Apr 23 13:16:18 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed Apr 23 13:23:42 2008 Subject: [Histonet] Automated Special Stainers In-Reply-To: <979FF5962E234F45B06CF0DB7C1AABB20FEC1F46@chi2k3ms01.columbuschildrens.net> References: <480F6FBA.1000303@pathology.washington.edu> <979FF5962E234F45B06CF0DB7C1AABB20FEC1F46@chi2k3ms01.columbuschildrens.net> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A747A23E@LTA3VS011.ees.hhs.gov> We really like our Artisan..... Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Wednesday, April 23, 2008 1:24 PM To: Victor Tobias; Histonet Subject: RE: [Histonet] Automated Special Stainers Nothing comes close as far as quality is concerned Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5450 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Wednesday, April 23, 2008 1:20 PM To: Histonet Subject: [Histonet] Automated Special Stainers Our PA who oversees Grossing and Histology asked me to post a question to the group. We currently have the Artisan (about 5 years d) and would like to know if there is something better for quality and output. Thanks Victor -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Wed Apr 23 14:27:37 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed Apr 23 14:27:58 2008 Subject: [Histonet] Paraformaldehyde use question for geniuses In-Reply-To: <1CE1847DFEA0A647B1CCDE4108EA60A747A23E@LTA3VS011.ees.hhs.gov> Message-ID: Gotcha. How long can tissues fixed in 4% PFA stay in PFA? If I can't process them for awhile, what should they be transferred to? Anyone know if 4%PFA is commercially available in vast quantities? Jackie O' From mcauliff <@t> umdnj.edu Wed Apr 23 14:42:01 2008 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Apr 23 14:41:54 2008 Subject: [Histonet] Paraformaldehyde use question for geniuses In-Reply-To: References: Message-ID: <480F9109.3060906@umdnj.edu> Hi Jackie: A few weeks will be OK. I would leave them in fix rather than 70% ethanol. I'm sure someone will sell you vast quantities of 4% PFA if you ask and have $$. Geoff Jackie M O'Connor wrote: > Gotcha. How long can tissues fixed in 4% PFA stay in PFA? If I can't > process them for awhile, what should they be transferred to? > Anyone know if 4%PFA is commercially available in vast quantities? > > Jackie O' > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From thisisann <@t> aol.com Wed Apr 23 15:15:43 2008 From: thisisann <@t> aol.com (thisisann@aol.com) Date: Wed Apr 23 15:15:51 2008 Subject: [Histonet] Grossing Qualifications Message-ID: <8CA7395F1657E24-1720-2DF7@WEBMAIL-MC20.sysops.aol.com> Can someone tell me where I can find CAP's grossing qualifications.? I need to know what degree and/or specific college courses need to be met in order to be qualified to gross (grandfather clause as well). Thank You, Ann Angelo AAngelo@QDxPath.com From NMargaryan <@t> childrensmemorial.org Wed Apr 23 16:53:35 2008 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Wed Apr 23 16:51:59 2008 Subject: [Histonet] IHC Staining Variations Message-ID: Trinity, Are you using a Lab Vision stainer? If so I am always having this problem, even I programmed 3-drop zones with 200ul of reagent dropping in each zone, for a total of 600ul per slide just to be sure that slide fully covered. We invited their tech several times. He checked run, clean and still sometimes I still have this kind of Staining Variations. I never had this problem before when used DAKO stainer. Please, let me know when and how did you fixed your problems. Good luck, Naira Naira V. Margaryan, D.V.M., Ph.D. Research Scientist Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3363 Tel: 773-755-6340 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 From victor <@t> pathology.washington.edu Wed Apr 23 17:22:12 2008 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Wed Apr 23 17:22:23 2008 Subject: [Histonet] Automated Special Stainers In-Reply-To: <1CE1847DFEA0A647B1CCDE4108EA60A747A23E@LTA3VS011.ees.hhs.gov> References: <480F6FBA.1000303@pathology.washington.edu> <979FF5962E234F45B06CF0DB7C1AABB20FEC1F46@chi2k3ms01.columbuschildrens.net> <1CE1847DFEA0A647B1CCDE4108EA60A747A23E@LTA3VS011.ees.hhs.gov> Message-ID: <480FB694.1090506@pathology.washington.edu> Thank you all for the quick responses, looks like we won't be switching any time soon. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Bartlett, Jeanine (CDC/CCID/NCZVED) wrote: > We really like our Artisan..... > > > Jeanine Bartlett > Infectious Diseases Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, > Ronald > Sent: Wednesday, April 23, 2008 1:24 PM > To: Victor Tobias; Histonet > Subject: RE: [Histonet] Automated Special Stainers > > Nothing comes close as far as quality is concerned > > > > Ronnie Houston, MS HT(ASCP)QIHC > > Anatomic Pathology Manager > > Nationwide Children's Hospital > > 700 Children's Drive > > Columbus, OH 43205 > > (614) 722 5450 > > ronald.houston@nationwidechildrens.org > > www.NationwideChildrens.org > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor > Tobias > Sent: Wednesday, April 23, 2008 1:20 PM > To: Histonet > Subject: [Histonet] Automated Special Stainers > > > > Our PA who oversees Grossing and Histology asked me to post a question > > to the group. We currently have the Artisan (about 5 years d) > > and would like to know if there is something better for quality and > output. > > > > Thanks > > Victor > > > > From suboro <@t> yahoo.com Wed Apr 23 17:26:20 2008 From: suboro <@t> yahoo.com (suboro) Date: Wed Apr 23 17:26:23 2008 Subject: [Histonet] opportunity in Honduras Message-ID: <738448.37350.qm@web34702.mail.mud.yahoo.com> Hello, 'Netters! A medical outreach group that is working in Honduras has an temporary (two month) position for a histo tech in beautiful Santa Rosa. This is a great opportunity for someone retired, between jobs, or with a ton of vacation time, to do some good in the world, have a great vacation, and earn some lempiras all at the same time. Please contact me for more information Susan Borocz HT(ASCP) suboro@yahoo.com --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From gorhamk <@t> verizon.net Wed Apr 23 18:15:59 2008 From: gorhamk <@t> verizon.net (Kathy Gorham) Date: Wed Apr 23 18:18:07 2008 Subject: [Histonet] unsubsribe Message-ID: <000601c8a597$fe83bbb0$2f01a8c0@kathy83b707eca> From c.malcontenti-wilson <@t> unimelb.edu.au Wed Apr 23 20:05:19 2008 From: c.malcontenti-wilson <@t> unimelb.edu.au (Cathy Malcontenti-Wilson) Date: Wed Apr 23 20:05:50 2008 Subject: [Histonet] VEGF R1/Flt-1 Immunostaining on FFPE mouse tissues Message-ID: <452655D3CC46464485FE5107C8723F2A020487CD@IS-EX-BEV1.unimelb.edu.au> Dear All, I would very much appreciate some help from someone who is doing/has done some immunostaining of FFPE mouse tissues with an antibody against VEGF Receptor 1/Flt-1. We are currently having difficulties believing the staining we are getting with the protocols we have tried. The antibody we have is Santa Cruz Flt-1 c-17 (sc-316). I would be grateful for any help. Thanks Cathy From jkiernan <@t> uwo.ca Thu Apr 24 00:06:20 2008 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Thu Apr 24 00:06:25 2008 Subject: [Histonet] Cornea nerve fibers Message-ID: Dear "Yaskovich, Ruth A (NIH/NIDCR) [E]"
The technical issu preparation: sections or whole-mounts. A cornea curved in 3D and it resists pressing to make a flat spe

John Kiernan
Anatomy, UWO
Londo Canada
----- Original Message -----
From: "Yaskov ich, Ruth A (NIH/NIDCR) [E]" Subject nerve fibers
To: Histonet

> Hi all, doe cornea?
>
> Ruth Yaskovich
National Institutes of Health
>
>
> _______ _______________________ 5F Histone Histonet@lists.utsouthwestern.edu< http://lists.utsouthwestern.edu/mailman/listinfo/his tonet

From Helen.Ilsley <@t> uct.ac.za Thu Apr 24 00:26:02 2008 From: Helen.Ilsley <@t> uct.ac.za (Helen Ilsley) Date: Thu Apr 24 00:26:09 2008 Subject: [Histonet] RECA-1 Message-ID: <9E250B99-D222-455D-9B63-75CF16536297@uct.ac.za> Hi I wonder if anyone out there is using RECA-1 on FFPE tissue using a fluorescent method. I am using it on rat heart. I can get it working on light but am battling to get a signal on IF. I would really appreciate it if anyone out there has managed to get this working for IF and could email me their protocol. Many thanks Helen Helen Ilsley Helen.Ilsley@uct.ac.za Cardiovascular Research Unit Cape Heart Centre Anzio Road, Observatory, UCT 021-406 6398/6590 021-448 5935 (fax) From relia1 <@t> earthlink.net Thu Apr 24 08:30:01 2008 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Apr 24 08:30:12 2008 Subject: [Histonet] Exciting management opportunity Message-ID: Hi Histonetters! I have a management position that I want to tell you about. This is a permanent full time Histology Supervisor position with a prestigious facility in the San Francisco Bay Area. This position is in a clinical environment. My client is looking for someone with a minimum of 5 years of experience in immunohistochemistry and histology supervision along with ASCP HT/HTL and the QIHC certification is preferred. My client offers excellent compensation and benefits. If you or someone you know might be interested in hearing more about this position please contact me. Incidentally I also have exciting management opportunities in Portland, OR, Los Angeles, CA and Dallas, TX and bench positions in CA, FL, TX, PA, VA, MD and WA. Have a great day!! Thank You! Pam M. Barker President Relia 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll Free: (866)607-3542 e-mail: relia1@earthlink.net http://home.earthlink.net/~relia1 www.myspace.com/pamatrelia From cmiller <@t> physlab.com Thu Apr 24 10:23:06 2008 From: cmiller <@t> physlab.com (Cheri Miller) Date: Thu Apr 24 10:22:14 2008 Subject: [Histonet] HT qualifications Message-ID: <000001c8a61f$1900d0e0$3d02a8c0@plab.local> I want to just get verification from the experts. I have an employee in Cyto -prep that wants to get into histology. She went to the local collage got information on obtaining an Associate in Science Degree. With that and 1 year of practical (OJT) in a histology lab she would be eligible for the HT exam, correct? I currently have 2 OJT techs I am training but they have a BA in the sciences already. I just wanted your opinions before I tell her to go ahead and enroll. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From gvdobbin <@t> ihis.org Thu Apr 24 10:26:17 2008 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Thu Apr 24 10:26:37 2008 Subject: [Histonet] pH probes and dyes-compatible? Message-ID: Hi Folks, I will need to use a pH meter in our Chemistry Lab to pH alizarin red. I would hate to ruin someone else's pH meter! Can anyone tell me if the repeated use of a pH probe for pH'ing dyes will affect the porosity of the glass probe or have any other detrimental affects for that matter? (I would use a pH meter far too infrequently to warrant maintaining one in my own lab.) Thanks. Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 There is some merit in doing the right thing rather badly, but absolutely none in doing the wrong thing excellently! ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- From crochieresteve <@t> aol.com Thu Apr 24 10:43:23 2008 From: crochieresteve <@t> aol.com (crochieresteve@aol.com) Date: Thu Apr 24 10:43:36 2008 Subject: [Histonet] H&E question Message-ID: <8CA743910799B39-125C-400@webmail-da20.sysops.aol.com> I am having issues with the surgipath 560mx hematoxylin. Goblet cells and mucin are staining blue. I have lowered the time to 1 min in Hematoxylin followed by 1 min in define and 1 min in bluing with wash steps in between, but the sections are still giving an over stained appearance. Would adding acetic acid to lower the pH help with this or should I try something different? steve From dencrowl <@t> MIT.EDU Thu Apr 24 12:38:18 2008 From: dencrowl <@t> MIT.EDU (Denise Crowley) Date: Thu Apr 24 12:38:29 2008 Subject: [Histonet] surgipath hematoxylin Message-ID: <2FCD05B5-53CE-40C9-BDB3-67481C863811@mit.edu> We tried the new Surgipath staining system, and while I was very happy with the stains, I found the Define to be ineffective at removing all of the background staining. So, I kept the Hematoxylin and Eosin/Phloxine and continued to use old-fashioned 1%HCl in 70% ethanol to clarify and Scott's tap water substitute to blue. Staining looks great, no need to filter Hematoxylin, and the stains are still working when I discard after ~2000 slides. Denise Crowley Facility Manager Histology David H. Koch Institute for Integrative Cancer Research Massachusetts Institute of Technology 40 Ames St. E17-427 Cambridge MA 02139 617-258-8183 dencrowl@mit.edu From mrsseagle <@t> yahoo.com Thu Apr 24 13:52:04 2008 From: mrsseagle <@t> yahoo.com (MICHELLE SEAGLE) Date: Thu Apr 24 13:52:14 2008 Subject: [Histonet] CE CREDITS?? Message-ID: <470026.42667.qm@web51807.mail.re2.yahoo.com> I am a newby to the registry and was wondering what some of you fellow HT'S were doing for your ce credits for the certificate maintenance program. Any suggestions would help. MICHELLE SEAGLE --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From mrstevens <@t> duckworthpathology.com Thu Apr 24 14:12:47 2008 From: mrstevens <@t> duckworthpathology.com (Mary Stevens) Date: Thu Apr 24 14:11:55 2008 Subject: [Histonet] microwave slide drying Message-ID: We have recently purchased a microwave from EBS to dry routine H&E slides. I would like to know what other users recommend for drying time and power settings. Thanks, Mary Stevens, HT(ASCP),QIHC(ASCP) Histology Supervisor Duckworth Pathology Group mrstevens@duckworthpathology.com 901-276-9192 From godsgalnow <@t> aol.com Thu Apr 24 14:16:27 2008 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Thu Apr 24 14:16:42 2008 Subject: [Histonet] artisan stainer Message-ID: <8CA7456D4257A0A-6CC-100C@webmail-da08.sysops.aol.com> Histonetters... Is anyone out there that is using the artisan special stainer willing to send me there spent cartridges for the alcian blue and pas stains?? Please let me know....I will even give you a fed -ex number to use when you send them. Thanks a bunch in advance Roxanne From Eric.Mahoney <@t> cchmc.org Thu Apr 24 14:49:05 2008 From: Eric.Mahoney <@t> cchmc.org (Eric Mahoney) Date: Thu Apr 24 14:49:29 2008 Subject: [Histonet] Re: Histonet Digest, Vol 53, Issue 34 Message-ID: <4810ABF10200009700014DBB@n6mcgw16.cchmc.org> Msg 20: H&E question (crochieresteve@aol.com) Did you try a 2 minute soak of slides in 250mL 1xPBS + 1mL 100% Conc. HCl after your Hematoxylin stain? Then run the slides gently under slowly running H20 (can be dDH20 or tap) for one minute and continue on with dehydrating and staining. >>> 04/24/08 1:01 PM >>> Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Automated Special Stainers (Victor Tobias) 2. Cornea nerve fibers (Paula Pierce) 3. RE: Automated Special Stainers (Houston, Ronald) 4. Free workshop (Shirley Powell) 5. IGF-IR antibody (Martha Ward) (Jean-Martin Lapointe) 6. RE: Automated Special Stainers (Bartlett, Jeanine (CDC/CCID/NCZVED)) 7. Paraformaldehyde use question for geniuses (Jackie M O'Connor) 8. Re: Paraformaldehyde use question for geniuses (Geoff McAuliffe) 9. Grossing Qualifications (thisisann@aol.com) 10. IHC Staining Variations (Margaryan, Naira) 11. Re: Automated Special Stainers (Victor Tobias) 12. opportunity in Honduras (suboro) 13. unsubsribe (Kathy Gorham) 14. VEGF R1/Flt-1 Immunostaining on FFPE mouse tissues (Cathy Malcontenti-Wilson) 15. Re: Cornea nerve fibers (John Kiernan) 16. RECA-1 (Helen Ilsley) 17. Exciting management opportunity (Pam Barker) 18. HT qualifications (Cheri Miller) 19. pH probes and dyes-compatible? (Greg Dobbin) 20. H&E question (crochieresteve@aol.com) ---------------------------------------------------------------------- Message: 1 Date: Wed, 23 Apr 2008 10:19:54 -0700 From: Victor Tobias Subject: [Histonet] Automated Special Stainers To: Histonet Message-ID: <480F6FBA.1000303@pathology.washington.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Our PA who oversees Grossing and Histology asked me to post a question to the group. We currently have the Artisan (about 5 years d) and would like to know if there is something better for quality and output. Thanks Victor -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. ------------------------------ Message: 2 Date: Wed, 23 Apr 2008 10:21:41 -0700 (PDT) From: Paula Pierce Subject: [Histonet] Cornea nerve fibers To: Histonet Message-ID: <996977.16027.qm@web50104.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 ?The cornea?only has unmyelinated?nerve fibers, so a luxol fast blue will not work. Try Rene's suggestion. ?Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 631 N. Broadway Ave. Moore, OK 73160 405-759-3953 lab contact@excaliburpathology.com www.excaliburpathology.com ------------------------------ Message: 3 Date: Wed, 23 Apr 2008 13:24:29 -0400 From: "Houston, RonSubject: RE: [Histonet] Automated Special Stainers To: "Victor Tobias" , "Histonet" Message-ID: <979FF5962E234F45B06CF0DB7C1AABB20FEC1F46@chi2k3ms01.columbuschildrens.net> Content-Type: text/plain; charset="us-ascii" Nothing comes close as far as quality is concerned Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5450 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Wednesday, April 23, 2008 1:20 PM To: Histonet Subject: [Histonet] Automated Special Stainers Our PA who oversees Grossing and Histology asked me to post a question to the group. We currently have the Artisan (about 5 years d) and would like to know if there is something better for quality and output. Thanks Victor -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ------------------------------ Message: 4 Date: Wed, 23 Apr 2008 13:21:43 -0400 From: Shirley Powell Subject: [Histonet] Free workshop To: histonet@lists.utsouthwestern.edu Message-ID: <01MTYRHM02P2002IFU@Macon2.Mercer.edu> Content-Type: text/plain; charset=us-ascii Hi Guys, Here is a free workshop with free CEUs for those in the Southeast who can get to Atlanta on May 21st. For more information go to our web site, GSH at www.hiistosearch.com/gsh, click on the education page or click on this link. http://www.histosearch.com/gsh/LeicaSymposium.pdf Shirley Powell GSH Secretary ------------------------------ Message: 5 Date: Wed, 23 Apr 2008 14:19:39 -0400 From: "Jean-Martin Lapointe" Subject: [Histonet] IGF-IR antibody (Martha Ward) To: Message-ID: Content-Type: text/plain; charset="us-ascii" I have no recent experience with IGF-1R IHC, but for such markers of cell function, in the past Cell Signaling has always been my first cho __________________________________ Jean-Martin Lapointe ------------------------------ Message: 6 Date: Wed, 23 Apr 2008 14:16:18 -0400 From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" Subject: RE: [Histonet] Automated Special Stainers To: "Houston, Ronald" , "Victor Tobias" , Histonet Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A747A23E@LTA3VS011.ees.hhs.gov> Content-Type: text/plain; charset=us-ascii We really like our Artisan..... Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Wednesday, April 23, 2008 1:24 PM To: Victor Tobias; Histonet Subject: RE: [Histonet] Automated Special Stainers Nothing comes close as far as quality is concerned Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5450 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Wednesday, April 23, 2008 1:20 PM To: Histonet Subject: [Histonet] Automated Special Stainers Our PA who oversees Grossing and Histology asked me to post a question to the group. We currently have the Artisan (about 5 years d) and would like to know if there is something better for quality and output. Thanks Victor -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Wed, 23 Apr 2008 14:27:37 -0500 From: Jackie M O'Connor Subject: [Histonet] Paraformaldehyde use question for geniuses To: Histonet , histonet-bounces@lists.uContent-Type: text/plain; charset="US-ASCII" Gotcha. How long can tissues fixed in 4% PFA stay in PFA? If I can't process them for awhile, what should they be transferred to? Anyone know if 4%PFA is commercially available in vast quantities? Jackie O' ------------------------------ Message: 8 Date: Wed, 23 Apr 2008 15:42:01 -0400 From: Geoff McAuliffe Subject: Re: [Histonet] Paraformaldehyde use question for geniuses To: Jackie M O'Connor Cc: Histonet , histonet-bounces@lists.utsouthwestern.edu Message-ID: <480F9109.3060906@umdnj.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hi Jackie: A few weeks will be OK. I would leave them in fix rather than 70% ethanol. I'm sure someone will sell you vast quantities of 4% PFA if you ask and have $$. Geoff Jackie M O'Connor wrote: > Gotcha. How long can tissues fixed in 4% PFA stay in PFA? If I can't > process them for awhile, what should they be transferred to? > Anyone know if 4%PFA is commercially available in vast quantities? > > Jackie O' > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** ------------------------------ Message: 9 Date: Wed, 23 Apr 2008 16:15:43 -0400 From: thisisann@aol.com Subject: [Histonet] Grossing Qualifications To: histonet@lists.utsouthwestern.edu Message-ID: <8CA7395F1657E24-1720-2DF7@WEBMAIL-MC20.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Can someone tell me where I can find CAP's grossing qualifications.? I need to know what degree and/or specific college courses need to be met in order to be qualified to gross (grandfather clause as well). Thank You, Ann Angelo AAngelo@QDxPath.com ------------------------------ Message: 10 Date: Wed, 23 Apr 2008 16:53:35 -0500 From: "Margaryan, Naira" Subject: [Histonet] IHC Staining Variations To: Message-ID: Content-Type: text/plain; charset="us-ascii" Trinity, Are you using a Lab Vision stainer? If so I am always having this problem, even I programmed 3-drop zones with 200ul of reagent dropping in each zone, for a total of 600ul per slide just to be sure that slide fully covered. We invited their tech several times. He checked run, clean and still sometimes I still have this kind of Staining Variations. I never had this problem before when used DAKO stainer. Please, let me know when and how did you fixed your problems. Good luck, Naira Naira V. Margaryan, D.V.M., Ph.D. Research Scientist Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3363 Tel: 773-755-6340 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 ------------------------------ Message: 11 Date: Wed, 23 Apr 2008 15:22:12 -0700 From: Victor Tobias Subject: Re: [Histonet] Automated Special Stainers To: "Bartlett, Jeanine (CDC/CCID/NCZVED)" Cc: Histonet Message-ID: <480FB694.1090506@pathology.washington.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Thank you all for the quick responses, looks like we won't be switching any time soon. Victor Victor Tobias Clinical Applications Analyst University206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Bartlett, Jeanine (CDC/CCID/NCZVED) wrote: > We really like our Artisan..... > > > Jeanine Bartlett > Infectious Diseases Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, > Ronald > Sent: Wednesday, April 23, 2008 1:24 PM > To: Victor Tobias; Histonet > Subject: RE: [Histonet] Automated Special Stainers > > Nothing comes close as far as quality is concerned > > > > Ronnie Houston, MS HT(ASCP)QIHC > > Anatomic Pathology Manager > > Nationwide Children's Hospital > > 700 Children's Drive > > Columbus, OH 43205 > > (614) 722 5450 > > ronald.houston@nationwidechildrens.org > > www.NationwideChildrens.org > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor > Tobias > Sent: Wednesday, April 23, 2008 1:20 PM > To: Histonet > Subject: [Histonet] Automated Special Stainers > > > > Our PA who oversees Grossing and Histology asked me to post a question > > to the group. We currently have the Artisan (about 5 years d) > > and would like to know if there is something better for quality and > output. > > > > Thanks > > Victor > > > > ------------------------------ Message: 12 Date: Wed, 23 Apr 2008 15:26:20 -0700 (PDT) From: suboro Subject: [Histonet] opportunity in Honduras To: histonet@lists.utsouthwestern.edu Message-ID: <738448.37350.qm@web34702.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello, 'Netters! A medical outreach group that is working in Honduras has an temporary (two month) position for a histo tech in beautiful Santa Rosa. This is a great opportunity for someone retired, between jobs, or with a ton of vacation time, to do some good in the world, have a great vacation, and earn some lempiras all at the same time. Please contact me for more information Susan Borocz HT(ASCP) suboro@yahoo.com --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. ------------------------------ Message: 13 Date: Wed, 23 Apr 2008 16:15:59 -0700 From: "Kathy Gorham" Subject: [Histonet] unsubsribe To: Message-ID: <000601c8a597$fe83bbb0$2f01a8c0@kathy83b707eca> Content-Type: text/plain; charset="iso-8859-1" ------------------------------ Message: 14 Date: Thu, 24 Apr 2008 11:05:19 +1000 From: Cathy Malcontenti-Wilson Subject: [Histonet] VEGF R1/Flt-1 Immunostaining on FFPE mouse tissues To: Histonet@lists.utsouthwestern.edu Message-ID: <452655D3CC46464485FE5107C8723F2A020487CD@IS-EX-BEV1.unimelb.edu.au> Content-Type: text/plain; charset="us-ascii" Dear All, I would very much appreciate some help from someone who is doing/has done some immunostaining of FFPE mouse tissues with an antibody against VEGF Receptor 1/Flt-1. We are currently having difficulties believing the staining we are getting with the protocols we have tried. The antibody we have is Santa Cruz Flt-1 c-17 (sc-316). I would be grateful for any help. Thanks Cathy ------------------------------ Message: 15 Date: Thu, 24 Apr 2008 0To: "Yaskovich, Ruth A (NIH/NIDCR) [E]" Cc: Histonet Message-ID: Content-Type: text/plain; charset="us-ascii" Dear "Yaskovich, Ruth A (NIH/NIDCR) [E]"
The technical issu preparation: sections or whole-mounts. A cornea curved in 3D and it resists pressing to make a flat spe

John Kiernan
Anatomy, UWO
Londo Canada
----- Original Message -----
From: "Yaskov ich, Ruth A (NIH/NIDCR) [E]" Subject nerve fibers
To: Histonet

> Hi all, doe cornea?
>
> Ruth Yaskovich
National Institutes of Health
>
>
> _______ _______________________ 5F Histone Histonet@lists.utsouthwestern.edu< http://lists.utsouthwestern.edu/mailman/listinfo/his tonet

------------------------------ Message: 16 Date: Thu, 24 Apr 2008 07:26:02 +0200 From: Helen Ilsley Subject: [Histonet] RECA-1 To: histonet@lists.utsouthwestern.edu Message-ID: <9E250B99-D222-455D-9B63-75CF16536297@uct.ac.za> Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed Hi I wonder if anyone out there is using RECA-1 on FFPE tissue using a fluorescent method. I am using it on rat heart. I can get it working on light but am battling to get a signal on IF. I would really appreciate it if anyone out there has managed to get this working for IF and could email me their protocol. Many thanks Helen Helen Ilsley Helen.Ilsley@uct.ac.za Cardiovascular Research Unit Cape Heart Centre Anzio Road, Observatory, UCT 021-406 6398/6590 021-448 5935 (fax) ------------------------------ Message: 17 Date: Thu, 24 Apr 2008 09:30:01 -0400 From: "Pam Barker" Subject: [Histonet] Exciting management opportunity To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Histonetters! I have a management position that I want to tell you about. This is a permanent full time Histology Supervisor position with a prestigious facility in the San Francisco Bay Area. This position is in a clinical environment. My client is looking for someone with a minimum of 5 years of experience in immunohistochemistry and histology supervision along with ASCP HT/HTL and the QIHC certification is preferred. My client offers excellent compensation and benefits. If you or someone you know might be interested in hearing more about this position please contact me. Incidentally I also have exciting management opportunities in Portland, OR, Los Angeles, CA and Dallas, TX and bench positions in CA, FL, TX, PA, VA, MD and WA. Have a great day!! Thank You! Pam M. Barker President Relia 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll Free: (866)607-3542 e-mail: relia1@earthlink.net http://home.earthlink.net/~relia1 www.myspace.com/pamatrelia ------------------------------ Message: 18 Date: Thu, 24 Apr 2008 10:23:06 -0500 From: "Cheri Miller" Subject: [Histonet] HT qualifications To: Message-ID: <000001c8a61f$1900d0e0$3d02a8c0@plab.local> Content-Type: text/plain; charset="us-ascii" I want to just get verification from the experts. I have an employee in Cyto -prep that wants to get into histology. She went to the local collage got information on obtaining an Associate in Science Degree. With that and 1 year of practical (OJT) in a histology laexam, correct? I currently have 2 OJT techs I am training but they have a BA in the sciences already. I just wanted your opinions before I tell her to go ahead and enroll. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ------------------------------ Message: 19 Date: Thu, 24 Apr 2008 12:26:17 -0300 From: "Greg Dobbin" Subject: [Histonet] pH probes and dyes-compatible? To: Message-ID: Content-Type: text/plain; charset=US-ASCII Hi Folks, I will need to use a pH meter in our Chemistry Lab to pH alizarin red. I would hate to ruin someone else's pH meter! Can anyone tell me if the repeated use of a pH probe for pH'ing dyes will affect the porosity of the glass probe or have any other detrimental affects for that matter? (I would use a pH meter far too infrequently to warrant maintaining one in my own lab.) Thanks. Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 There is some merit in doing the right thing rather badly, but absolutely none in doing the wrong thing excellently! ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- ------------------------------ Message: 20 Date: Thu, 24 Apr 2008 11:43:23 -0400 From: crochieresteve@aol.com Subject: [Histonet] H&E question To: histonet@pathology.swmed.edu Message-ID: <8CA743910799B39-125C-400@webmail-da20.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" I am having issues with the surgipath 560mx hematoxylin. Goblet cells and mucin are staining blue. I have lowered the time to 1 min in Hematoxylin followed by 1 min in define and 1 min in bluing with wash steps in between, but the sections are still giving an over stained appearance. Would adding acetic acid to lower the pH help with this or should I try something different? steve ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 53, Issue 34 **************************************** From rjbuesa <@t> yahoo.com Thu Apr 24 14:58:22 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Apr 24 14:58:25 2008 Subject: [Histonet] microwave slide drying In-Reply-To: Message-ID: <243116.11428.qm@web65706.mail.ac4.yahoo.com> Both will depend on the MW wattage output. The best thing to do is to run some tests, and remember that the more slides you are going to dry, the more time or more power you will need. Ren? J. Mary Stevens wrote: We have recently purchased a microwave from EBS to dry routine H&E slides. I would like to know what other users recommend for drying time and power settings. Thanks, Mary Stevens, HT(ASCP),QIHC(ASCP) Histology Supervisor Duckworth Pathology Group mrstevens@duckworthpathology.com 901-276-9192 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From b-frederick <@t> northwestern.edu Thu Apr 24 15:04:39 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Apr 24 15:04:46 2008 Subject: [Histonet] Job opening Message-ID: <000701c8a646$6d94a9c0$d00f7ca5@lurie.northwestern.edu> Hello all, We have a position open for a certified histotech. For those of you who area aware of who and what ECOg is,we are their reference lab. We receive blocks and slides from over 200 insittutions within the US and overseas. We cut a lot and do centralized testing for a vast number of cancer clinical trials including IHC,FISH, blot and DNA/RNA extraction among other things. We are a tissue bank under the auspices of the NIH/NCI . The Pathcore Facility is part of the Robert H. Lurie Caner center which in turn is part of Northwestern University. Our closest affliated hospital is Northwestern Memorial hospital. We are the only lab of our kind. We are located in the heart of the Gold Coast of Chicago,2 blocks from the lake and 2 blocks from Michigan Avenue. Lots of public transporataion in the city and out to the suburbs (I'm 36 miles out and take a train in as do many employees) We deal in high volumes of tissue and much of it is protocol specific. If anyone is interested, please e-mail me and I can give you the info for applying etc. It is electronic. Thanks, Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility/ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 From TJJ <@t> Stowers-Institute.org Thu Apr 24 15:28:55 2008 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Thu Apr 24 15:29:16 2008 Subject: [Histonet] Thank you Gayle Callis! Message-ID: I was just called by a histology colleague who was asking me a question about GFP fluorescence. Some of the wisdom I imparted was taken directly from the experience of Gayle Callis, who unselfishly shared it with this list. She is one of the biggest contributors here, and I just wanted to say Thank You to Gayle for all of your help, advice, and expertise throughout the years. Learning from her has made me smarter. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From gayle.callis <@t> bresnan.net Thu Apr 24 15:55:13 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Thu Apr 24 15:55:11 2008 Subject: [Histonet] pH probes and dyes-compatible? References: Message-ID: <002201c8a64d$7dfa2fe0$6501a8c0@DHXTS541> Greg, What you should do it provide your own electrode, a single/combination plastic barrel that can be interchanged with their pH meter (check specifications). Just make sure this electrode is compatible with TRIS buffers too, you may need to pH one of those sometime. This way you don't anger the chem people if any residual dye is clinging to the electrode. The combination plastic electrodes are NOT expensive, and to maintain them, store in the pH 4 buffer used to calibrate the meter. These electrodes often have a protective sleeve down over the leads, so sitting them in a flask with storage pH 4 buffer (stable, cheap under vendor name/labels) will not damage the bottom where leads are located during stirring or storage. The pH 4 buffer supposedly keeps the openings to leads from plugging up. We get slimy stuff growing in distilled water storage, but less so than pH 4 buffer - be sure to rinse this away with distilled water before pH adjustment/readings. After you trash the plastic barrel electrode with stains (and not the chemistry lab's precious electrodes), just replace with another, cheap electrode. We were advised of this years ago, and it worked for us. It may be in your best interest to buy an electrode stand - there are some clever ones that have flexible arms to free up your hands for pH adjustment and when attaching your electrode to the meter. Our electrode is long and skinny, to reach into narrow, but large flasks or bottles for solution preparations e.g. pH adjustment of 14% tetrasodium EDTA decalcifiying solution - 2 liters or more at a time. We thought we would not need a pH meter in the histolab, but it has been a godsend for over 20 years. There are some inexpensive, reliable meters out there. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Greg Dobbin" To: Sent: Thursday, April 24, 2008 9:26 AM Subject: [Histonet] pH probes and dyes-compatible? Hi Folks, I will need to use a pH meter in our Chemistry Lab to pH alizarin red. I would hate to ruin someone else's pH meter! Can anyone tell me if the repeated use of a pH probe for pH'ing dyes will affect the porosity of the glass probe or have any other detrimental affects for that matter? (I would use a pH meter far too infrequently to warrant maintaining one in my own lab.) Thanks. Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 There is some merit in doing the right thing rather badly, but absolutely none in doing the wrong thing excellently! ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJJ <@t> Stowers-Institute.org Thu Apr 24 15:55:20 2008 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Thu Apr 24 15:55:53 2008 Subject: [Histonet] Re: Paraformaldehyde use question for geniuses Message-ID: Geoff, Have you performed experiments that show more of a benefit for storing samples in formalin over 70% alcohol in your lab? I have one researcher who, based on their experience in cultured cells, knows that anything over 10 minutes of fixation time causes their antibody staining to diminish using one particular antibody. I'm well aware of the current trend towards longer fixation times and generally believe that more is better. Because of evidence to the contrary, I cannot bring myself to recommend as a matter of routine, to store samples in formalin, especially for research purposes. I much prefer to have the samples paraffin processed or cryo embedded after a defined period of time in fixative, and store the tissues in those two forms rather than anything aqueous or alcoholic. Jackie - I'm not aware of any vendors who sell 4% formalin made from PFA in large quantities, simply due to the problem of re-polymerization upon storage. We get 16% solution in ampules, and then dilute in PBS to 4%. Perhaps that might work for you? Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From bakevictoria <@t> gmail.com Thu Apr 24 16:28:17 2008 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Thu Apr 24 16:28:27 2008 Subject: [Histonet] Thank you Gayle Callis! In-Reply-To: References: Message-ID: <4f016b690804241428p4c1c199fya5259a6d3e7698cc@mail.gmail.com> I'll 2nd, 3rd, 4th etc that as Gayle has and still is very much a sounding board for so many of us. On or Off - Topic, routine or off the wall questions about procedures, results, weird requests from Pathologists or PI's, Gayle always gives her best to us. What I most admire and respect is that she never is negative, always positive and informative - forces me (at least) to think 'outside of the box' and offers a helping hand with a sense of humor that I wish I had. Vikki Baker On 4/24/08, Johnson, Teri wrote: > I was just called by a histology colleague who was asking me a question about GFP fluorescence. Some of the wisdom I imparted was taken directly from the experience of Gayle Callis, who unselfishly shared it with this list. She is one of the biggest contributors here, and I just wanted to say Thank You to Gayle for all of your help, advice, and expertise throughout the years. > > Learning from her has made me smarter. > > Teri Johnson, HT(ASCP)QIHC > Managing Director Histology Facility > Stowers Institute for Medical Research > 1000 E. 50th St. > Kansas City, MO 64110 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gayle.callis <@t> bresnan.net Thu Apr 24 17:02:27 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Thu Apr 24 17:07:42 2008 Subject: [Histonet] Re: Paraformaldehyde use question for geniuses References: Message-ID: <005b01c8a656$e25579f0$6501a8c0@DHXTS541> Dear Teri and Fellow Histonetters, Teri's words of wisdom on fixation and storage of samples are well taken. Please provide the vendor for the 16% paraformaldehyde in ampules so our FACS technician doesn't have to weigh out PFA powder anymore. She is going to be delighted. Gayle M. Callis ----- Original Message ----- From: "Johnson, Teri" To: Sent: Thursday, April 24, 2008 2:55 PM Subject: [Histonet] Re: Paraformaldehyde use question for geniuses Geoff, Have you performed experiments that show more of a benefit for storing samples in formalin over 70% alcohol in your lab? I have one researcher who, based on their experience in cultured cells, knows that anything over 10 minutes of fixation time causes their antibody staining to diminish using one particular antibody. I'm well aware of the current trend towards longer fixation times and generally believe that more is better. Because of evidence to the contrary, I cannot bring myself to recommend as a matter of routine, to store samples in formalin, especially for research purposes. I much prefer to have the samples paraffin processed or cryo embedded after a defined period of time in fixative, and store the tissues in those two forms rather than anything aqueous or alcoholic. Jackie - I'm not aware of any vendors who sell 4% formalin made from PFA in large quantities, simply due to the problem of re-polymerization upon storage. We get 16% solution in ampules, and then dilute in PBS to 4%. Perhaps that might work for you? Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJJ <@t> Stowers-Institute.org Thu Apr 24 17:16:23 2008 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Thu Apr 24 17:16:52 2008 Subject: [Histonet] Re: Paraformaldehyde use question for geniuses In-Reply-To: <005b01c8a656$e25579f0$6501a8c0@DHXTS541> Message-ID: Gayle, we can get it from VWR 10 vials of 10 ml per each, Vendor #AA43368-9M Our EM dept. gets theirs from Electron Microscopy Sciences, 10 vials of 10 ml per each, EM Grade, Vendor #15710 It's one of the best things I've done for the lab. Teri -----Original Message----- From: Gayle Callis [mailto:gayle.callis@bresnan.net] Sent: Thursday, April 24, 2008 5:02 PM To: Johnson, Teri; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Paraformaldehyde use question for geniuses Dear Teri and Fellow Histonetters, Teri's words of wisdom on fixation and storage of samples are well taken. Please provide the vendor for the 16% paraformaldehyde in ampules so our FACS technician doesn't have to weigh out PFA powder anymore. She is going to be delighted. Gayle M. Callis From lpwenk <@t> sbcglobal.net Thu Apr 24 19:40:44 2008 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Apr 24 19:40:59 2008 Subject: [Histonet] CE CREDITS?? In-Reply-To: <470026.42667.qm@web51807.mail.re2.yahoo.com> Message-ID: <003601c8a66c$ff69ed30$0202a8c0@HPPav2> Congratulations on passing the registry exam. Now comes the life-time of learning (and documenting), Go to the ASCP Board of Registry web page, and open up the booklet on CMP (certification maintenance program) http://www.ascp.org/FunctionalNavigation/certification/CertificationMaintena nceProgramCMP.aspx Look at pages 4-6, for ideas. 1. Attend state and national meetings. If you are not a member of your state society or NSH - JOIN! If you are not a member of ASCP - JOIN! They can help with your CE. ASCP: http://www.ascp.org/MainMenu/laboratoryprofessionals/Membership.aspx NSH: www.nsh.org Click on Membership on the left If you don't know how to contact your state society, go to the NSH website, and click on Resources for State Societies on the left, and click on the president of your state for an email. When you attend a state or NSH meeting, you earn 1 hour CE for each hour of talk/workshop you attend. (#1 on CMP chart) *BTW - there are NSH scholarship awards that you can apply for, to you could use to pay to attend some conventions. 2. Get in-house in-service talks. - Get your pathologists to give talks once a month. - Get other people in your hospital to give talks - someone from the breast cancer institute, the transplant team, the safety department, pastoral care (religion and the laboratory), someone from management, etc. - Get vendors to come give talks at your lab. Microtome, tissue processor, automated staining, microwave processing, recycling, etc. Just have sign-in sheets with date, time (10 am - 11 am = 1 hour CE), topic, location. (1 hour lab related talk = 1 hour CMP) (#2 on CMP chart) 3. Continue to take college classes (science, management, computers, etc.). 1 semester hour = 15 hours CMP; or a 4 credit class = 60 hours CMP (#3 on CMP list) 4. Get the lab to sign up for teleconferences: ASCP NSH TNT = Teleconference Network of Texas All three have histology and other related teleconferences. 1 hour teleconference = 1 hour CMP (#4 on CMP list) 5. Take the tests found in journals and magazines. - NSH has tests for one article in each Journal of Histotechnology - Advance for Medical Laboratory Sciences has tests on an article periodically. Free to subscribe. Or go to webpage, print off article and test. (Called Learning Scope). Fee to submit. http://laboratorian.advanceweb.com/ - Medical Laboratory Observer (MLO)is geared more for supervisors. Free to subscribe, or go to webpage and print off articles and test. Fee to submit. http://www.mlo-online.com/ (#4 of CMP list) 6. Take on-line CE courses - Lab Vision through Thermo has tutorials approved by NSH http://www.labvision.com/indexTutorial.cfm - If you are an ASCP member, there are courses you can take that are free. Some you have to pay a fee for. http://www.ascp.org/ASCPStore/Store/eLearning.aspx (#4 of CMP list) 7. Competency assessment Since your supervisor has to do competency assessment (CAP, JCAHO requirements), pull up the form from the ASCP CMP page, and have them fill it out. This is worth 2 CMP/year. (# 6 of CMP list) 8. Present a talk at a state (or NSH) meeting. YES, you ARE qualified to give a talk. Pick a topic that is of interest to you. Read everything you can on it. Put together a PowerPoint with handouts. And every state is BEGGING for people to agree to present. Very few presenters are THE expert on the topic they are talking about. They have taken the time to learn a bunch on the topic, and are WILLING to stand up and present. 1 hour presentation = 3 hour CMP (#7 on CMP list) There are LOTS of ways to earn 12 CEU hours each year (36 over 3 years). But you have to sign up, maybe pay some money, give up some of your own time. But it's better than losing your ASCP certification, having to study all over again, pay the application fee again, etc. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MICHELLE SEAGLE Sent: Thursday, April 24, 2008 2:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CE CREDITS?? I am a newby to the registry and was wondering what some of you fellow HT'S were doing for your ce credits for the certificate maintenance program. Any suggestions would help. MICHELLE SEAGLE --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tissuearray <@t> hotmail.com Thu Apr 24 20:21:18 2008 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Thu Apr 24 20:21:23 2008 Subject: [Histonet] Help In-Reply-To: References: Message-ID: Sounds like you are in Salt Lake City, Ut. Try Janet Hansen at the IMC EM Lab in Murray. They do EMs on an almost daily basis. Good luck, Thom > Date: Tue, 22 Apr 2008 08:30:54 -0600 > From: kenneth.metzger@aruplab.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Help > > Is anyone or does anyone know a good EM contact whose brain I could pick? Thanks, > > Ken > > Ken Metzger HTL(ASCP) > Histology Supervisor > ARUP Laboratories > 500 Chipeta way > Salt Lake City, UT 84108 > 801.583.2787 ext 3101 > > > - ------------------------------------------------------------------ > The information transmitted by this e-mail and any included > attachments are from ARUP Laboratories and are intended only for the > recipient. The information contained in this message is confidential > and may constitute inside or non-public information under > international, federal, or state securities laws, or protected health > information and is intended only for the use of the recipient. > Unauthorized forwarding, printing, copying, distributing, or use of > such information is strictly prohibited and may be unlawful. If you > are not the intended recipient, please promptly delete this e-mail > and notify the sender of the delivery error or you may call ARUP > Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 > (800) 522-2787 ext. 2100 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Express yourself wherever you are. Mobilize! http://www.gowindowslive.com/Mobile/Landing/Messenger/Default.aspx?Locale=en-US?ocid=TAG_APRIL From sheila_adey <@t> hotmail.com Thu Apr 24 20:40:22 2008 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Thu Apr 24 20:40:27 2008 Subject: [Histonet] IHC Staining Variations In-Reply-To: <16C83872A53F4346AA9C3A18E3A3AAB903F76D81@VHAV10MSGA1.v10.med.va.gov> References: <8CA6ED028A45C69-52C-95F@webmail-nd11.sysops.aol.com> <16C83872A53F4346AA9C3A18E3A3AAB903F76D81@VHAV10MSGA1.v10.med.va.gov> Message-ID: We had similar problems and noticed that our reagents were not "spreading" equally across the slide. The manufacturer (Biogenex) protocol was to use PBS. We tried the Dakko TRIS and it covers easily and allows the antibody to spread evenly. Hope this helps.Sheila Adey HT MLTPort Huron HospitalMichigan> Date: Thu, 17 Apr 2008 17:45:10 -0400> From: Susan.Weber2@va.gov> To: histobeach@aol.com; histonet@lists.utsouthwestern.edu> Subject: RE: [Histonet] IHC Staining Variations> CC: > > Trinity-> Are you using the Ventana Benchmark XT equipment? I recently started> using this equipment and in our training class they told us that certain> slides can be hydrophobic and using a Pap Pen can also effect the> staining quality. I switched from Surgipath X slides to Fisher> Superfrost/Plus slides for this very reason. I use the Shandon Double> slide with the rings on them for our FITC stains and did not have a> problem - the ring is on the underside of the slide, not the side you> put the tissue on. Some of the slides which have "boxes" on them also> affect staining quality (unless it is on the underside of the slide of> course). If you are using the Ventana equipment, their Tech Support is> very good and can help you. Even if your not using this equipment, I'd> call the Tech support of your manufacturer and ask for their help.> > > Susan M Weber HT(ASCP)> Histology Supervisor> Louis Stokes Cleveland VA Medical Center> 10701 East Blvd> Cleveland, Ohio 44106> (216) 791-3800 X6154> > -----Original Message-----> From: histonet-bounces@lists.utsouthwestern.edu> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of> histobeach@aol.com> Sent: Thursday, April 17, 2008 2:30 PM> To: histonet@lists.utsouthwestern.edu> Subject: [Histonet] IHC Staining Variations> > Hello,> > We have an automated IHC stainer and the doctors are experiencing what> they call, staining variations.? The whole tissue is not staining, just> in parts, when areas of the tissues should be staining.? > > Has anyone experienced this problem and if so, what did you do to remedy> it?? We were staining manually and used a Pap Pen before we switched to> automation and I'm wondering if we are getting areas on the slide that> are not being covered with the reagents.? We have 3 drop zones with> 100ul of reagent dropping in each zone, for a total of 300ul per slide.> > I appreciate any feedback and help.? > > Trinity Whitecomb> Histology Manager> Lab System Works> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Enter today for your chance to win $1000 a day?today until May 12th. Learn more at SignInAndWIN.ca http://g.msn.ca/ca55/215 From ks_andthe4foots <@t> yahoo.com Thu Apr 24 21:57:38 2008 From: ks_andthe4foots <@t> yahoo.com (Steven Caokley) Date: Thu Apr 24 21:57:50 2008 Subject: [Histonet] HT availiable to help. Message-ID: <164513.37789.qm@web45712.mail.sp1.yahoo.com> I'm semi retired [27.gif] but would like to help out in HT labs as needed [21.gif] . I know how long term short staffing can effect the health [26.gif] of my fellow professionals. 2 obstacle's: fuel cost [34.gif] and high priced staffing agencies. [31.gif] If interested contact me and I'll get you my resume and we'll go from there. [14.gif] _________________________________________________________________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. [1]Try it now. References 1. http://us.rd.yahoo.com/evt=51733/*http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ From Melker.Goransson <@t> astrazeneca.com Fri Apr 25 02:08:00 2008 From: Melker.Goransson <@t> astrazeneca.com (=?iso-8859-1?Q?=22G=F6ransson=2C_Melker=22?=) Date: Fri Apr 25 02:08:08 2008 Subject: [Histonet] VEGF R1/Flt-1 Immunostaining on FFPE mouse tissues In-Reply-To: <452655D3CC46464485FE5107C8723F2A020487CD@IS-EX-BEV1.unimelb.edu.au> Message-ID: <64A531C5BF0A1F4DA4D02849B43FEF370111FA9A@SEMLRDEMBX01.rd.astrazeneca.net> Hi Cathy, A couple of years ago I did some FLT1 stainings on FFPE mouse xenografts of human tumor cells. We were also having difficulties to believe the staining as it was mainly localized to the nucleus. My solution was to use the same human tumor cells in tissue culture, separate the nuclear and cytoplasmic proteins and run a Western. This showed that the signal from the nucleus was specific. Similar findings for EGFR has been reported by others: Lo HW, Ali-Seyed M, Wu Y, Bartholomeusz G, Hsu SC, Hung MC. Nuclear-cytoplasmic transport of EGFR involves receptor endocytosis, importin beta1 and CRM1. J Cell Biochem. 2006 Mar 21. I don't know if this will help your interpretation, but it might be good to have in mind. Cheers/ Melker -----Original Message----- From: Cathy Malcontenti-Wilson [mailto:c.malcontenti-wilson@unimelb.edu.au] Sent: torsdag, april 24, 2008 03:05 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] VEGF R1/Flt-1 Immunostaining on FFPE mouse tissues Dear All, I would very much appreciate some help from someone who is doing/has done some immunostaining of FFPE mouse tissues with an antibody against VEGF Receptor 1/Flt-1. We are currently having difficulties believing the staining we are getting with the protocols we have tried. The antibody we have is Santa Cruz Flt-1 c-17 (sc-316). I would be grateful for any help. Thanks Cathy From John.Spair <@t> multicare.org Fri Apr 25 02:44:12 2008 From: John.Spair <@t> multicare.org (John Spair) Date: Fri Apr 25 02:44:52 2008 Subject: [Histonet] Bone Marrows References: <640F5C3A2G041498-01@MMS_multicare.org> Message-ID: <61A9977919846C479389493BAE2517CA01530921@MHSEXMBX1.multicare.org> Up until about a year ago, we used to use B-5 fixative for our bone marrow biopsies and always had wonderful results. However like everyone else, we had to eliminate anything with mercury in it and began using various zinc chloride solutions from various vendors for both lymph nodes and bone marrows. Well our docs weren't happy at all and we never found anything that equaled the quality of B-5. Recently we went back to just plain old 10% NBF, but I hear the pathologist's comments from time to time about how they miss B-5. I tried to get special permission to use B-5 once again but only open up a can of worms about that topic. So I wonder, has anyone ever found a suitable replacement that equals the quality of good old mercuric chloride??? John Spair, Manager - Pathology Services LABORATORIES Northwest - MultiCare Health System Phone: 253-403-6090 Fax: 253-403-1357 "MMS " made the following annotations. ------------------------------------------------------------------------------ NOTICE: This e-mail and the attachments hereto, if any, may contain privileged and/or confidential information. It is intended only for use by the named addressee(s). If you are not the intended recipient of this e-mail, you are hereby notified that any examination, distribution or copying of this e-mail and the attachments hereto, if any, is strictly prohibited. If you have received this transmission in error, please immediately notify the sender by email or telephone and permanently delete this e-mail and the attachments hereto, if any, and destroy any printout thereof. MultiCare Health System, Tacoma, WA 98415 (253) 403-1000. ============================================================================== From sheila_adey <@t> hotmail.com Fri Apr 25 06:47:57 2008 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Fri Apr 25 06:48:02 2008 Subject: [Histonet] Looking for a weekend Histo job in the Detroit, or London Ontario area Message-ID: I'm looking for an extra shift on the weekends in either the Detroit area or London Ontario area. I have a current CMLTO registration. Thanks Sheila Adey HT MLTPort Huron HospitalMichigan _________________________________________________________________ Try Chicktionary, a game that tests how many words you can form from the letters given. Find this and more puzzles at Live Search Games! http://g.msn.ca/ca55/207 From rjbuesa <@t> yahoo.com Fri Apr 25 07:11:03 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Apr 25 07:11:10 2008 Subject: [Histonet] Bone Marrows In-Reply-To: <61A9977919846C479389493BAE2517CA01530921@MHSEXMBX1.multicare.org> Message-ID: <195252.72753.qm@web65702.mail.ac4.yahoo.com> I always used (even before B5 was totally banned) NBF but with special precautions regarding the pH that was checked for each batch and also all the Giemsa staining was done with pH controlled phosphate buffers. We never had a problem, nor a complaint from the pathologists. I advise you "to follow that path". Ren? J. John Spair wrote: --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From ccrowder <@t> vetmed.lsu.edu Fri Apr 25 07:20:26 2008 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Fri Apr 25 07:24:13 2008 Subject: [Histonet] Drying slides in MW Message-ID: I am not familiar with the EMS MW, but normally drying if your stained slides are on a cardboard slide tray (holding 20), they can be put in the MW, heated on full power for 1 minute, removed and cooled for 5 minutes. The slides are not ready to be filed, but the coverglass will not move around. The only down slide to this method is the slide tray itself. Being layers of cardboard, when placed warm on a counter the top cools faster than the underside. This can cause the tray to warp. We place our trays on coplin jars to cool them so the top and bottom cool at the same rate and no bowing occurs. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 From relia1 <@t> earthlink.net Fri Apr 25 07:39:45 2008 From: relia1 <@t> earthlink.net (Pam Barker) Date: Fri Apr 25 07:39:54 2008 Subject: [Histonet] RELIA Histology Job Alert 4/25/08 Always wanted to get into research?? Now's your chance!! Message-ID: Hi Histonetters!! I hope everybody is getting ready for a beautiful Spring weekend!! Before you go off to enjoy please take a second and take a look. I am currently working with a pre-clinical research firm who is in need of several histo techs for their lab. These are permanent full time dayshift positions. My client offers excellent salary, benefits and relocation assistance. The requirements are ASCP HT/HTL or eligible, a minimum of 1 year of experience OR successful completion of an accredited histology program. Yes. New Grads are welcome!!! If you are a student getting ready to graduate you are welcome to apply as well!! The positions are located in Seattle WA. If you or someone you know might be interested please contact me. I can be reached toll free at 866-607-3542 or relia1@earthlink.net Remember it never hurts to look!! Thanks for taking the time to read my post and Have a great weekend!! Thank You! Pam M. Barker President Relia 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll Free: (866)607-3542 e-mail: relia1@earthlink.net http://home.earthlink.net/~relia1 www.myspace.com/pamatrelia From annigyg <@t> gmail.com Fri Apr 25 07:41:00 2008 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Fri Apr 25 07:41:04 2008 Subject: [Histonet] Bone Marrows In-Reply-To: <195252.72753.qm@web65702.mail.ac4.yahoo.com> References: <61A9977919846C479389493BAE2517CA01530921@MHSEXMBX1.multicare.org> <195252.72753.qm@web65702.mail.ac4.yahoo.com> Message-ID: we have been through the same process (3 years of painful persuasion) and finally the paths have been silenced (well, most of them) - we use B plus fixative its great and we are now totally mercury free nothing can compare to B5 but in this day and age it is senseless to even contemplate using mercury in the lab again Annie (in arabia) greetings from Abu Dhabi!! 2008/4/25 Rene J Buesa : > I always used (even before B5 was totally banned) NBF but with special > precautions regarding the pH that was checked for each batch and also all > the Giemsa staining was done with pH controlled phosphate buffers. We never > had a problem, nor a complaint from the pathologists. > I advise you "to follow that path". > Ren? J. > > John Spair wrote: > > > > --------------------------------- > Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it > now. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne (van Binsbergen) Hope Abu Dhabi UAE From mcauliff <@t> umdnj.edu Fri Apr 25 08:41:47 2008 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Apr 25 08:41:40 2008 Subject: [Histonet] Re: Paraformaldehyde use question for geniuses In-Reply-To: References: Message-ID: <4811DF9B.1030203@umdnj.edu> Hi Teri: The original question did not mention IHC so I did not take that into account. Geoff Johnson, Teri wrote: > Geoff, > > Have you performed experiments that show more of a benefit for storing samples in formalin over 70% alcohol in your lab? I have one researcher who, based on their experience in cultured cells, knows that anything over 10 minutes of fixation time causes their antibody staining to diminish using one particular antibody. I'm well aware of the current trend towards longer fixation times and generally believe that more is better. Because of evidence to the contrary, I cannot bring myself to recommend as a matter of routine, to store samples in formalin, especially for research purposes. > > I much prefer to have the samples paraffin processed or cryo embedded after a defined period of time in fixative, and store the tissues in those two forms rather than anything aqueous or alcoholic. > > Jackie - I'm not aware of any vendors who sell 4% formalin made from PFA in large quantities, simply due to the problem of re-polymerization upon storage. We get 16% solution in ampules, and then dilute in PBS to 4%. Perhaps that might work for you? > > Teri Johnson, HT(ASCP)QIHC > Managing Director Histology Facility > Stowers Institute for Medical Research > 1000 E. 50th St. > Kansas City, MO 64110 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From JMaslanka <@t> stpetes.org Fri Apr 25 09:27:09 2008 From: JMaslanka <@t> stpetes.org (JMaslanka@stpetes.org) Date: Fri Apr 25 09:28:16 2008 Subject: [Histonet] Available Position Message-ID: Montana, mountains, rivers, big sky, yes the great outdoors. St Peters Hospital has a histology position open. If interested check out www.stpetes.org Joe Maslanka Cyto/Histo Coord. St Peter's Laboratory " Not everything that can be counted counts..... Not everything that counts can be counted." Albert Einstein From Maxim_71 <@t> mail.ru Fri Apr 25 11:55:56 2008 From: Maxim_71 <@t> mail.ru (Maxim_71@mail.ru) Date: Fri Apr 25 12:04:21 2008 Subject: [Histonet] microwave slide drying Message-ID: <1159311467.20080425205556@mail.ru> Mary, This is sounds like shoot from gun on sparrows. The Magnetron is a powerful source to energy, but cuts have a small area and are a bad receiver for it. The paraffin much well misses microwaves, since water is not. That is why water quickly boils from beneath cut. Electric ventilator will better suit for purpose fast drying. We uses for drying slides conventional oven. Please check Histonet archives (January 2006), that this issue discussed. What will say the vendors of microwave equipment? Maxim Peshkov, Russia, Taganrog. mailto:Maxim_71@mail.ru From RSRICHMOND <@t> aol.com Fri Apr 25 12:41:26 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Fri Apr 25 12:41:51 2008 Subject: [Histonet] Re: Bone Marrows Message-ID: We all knew and loved mercury-containing B-5 fixative, but it's gone and it's not coming back. I've never found the zinc fixatives to be of the slightest use. Remember also that immunostains often depend on neutral buffered formalin fixation. Neutral buffered formalin requires overnight fixation of bone marrow specimens, and decalcification has to be timed properly. Sections of bone marrow are of more and more diagnostic importance, since more and more oncologists and medical technologists cannot prepare a bone marrow smear, so that the pathologist has to depend on tissue sections to make a diagnosis. Bob Richmond Samurai Pathologist Knoxville TN From JHAPPEL <@t> PARTNERS.ORG Fri Apr 25 13:17:51 2008 From: JHAPPEL <@t> PARTNERS.ORG (Happel, James F.) Date: Fri Apr 25 13:17:55 2008 Subject: [Histonet] Please Remove Me From This List Message-ID: Good Afternoon, Please remove me from the Histonet for now. Thank you. James F. Happel, DLM (ASCP) HTL Technical Director of Surgical Pathology Massachusetts General Hospital 55 Fruit Street Boston, Massachusetts 02114 ph. (617) 726-5153 fax (617) 726-6829 The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From bliven.laura <@t> marshfieldclinic.org Fri Apr 25 15:24:59 2008 From: bliven.laura <@t> marshfieldclinic.org (Bliven, Laura) Date: Fri Apr 25 15:25:05 2008 Subject: [Histonet] Myoglobin Polyclonal Antibody Message-ID: <200804252025.m3PKP1HN004660@spamfilt.mfldclin.edu> My rabbit must have died. I'm looking for a concentrate, IVD or ASR classification, Polyclonal, Myoglobin Antibody. I've check a few places, but some companies have just recently discontinued this antibody. I'm sure a rabbit monoclonal would also work nice. The group here really likes to stick to IVD or ASR's. Thanks, Laura Laura Bliven Histology Lab/IHC Marshfield Laboratories 1000 N. Oak Ave. Marshfield, WI 54449 From collette2 <@t> mail.llnl.gov Fri Apr 25 15:51:39 2008 From: collette2 <@t> mail.llnl.gov (Nicole Collette) Date: Fri Apr 25 15:43:15 2008 Subject: [Histonet] Re: Paraformaldehyde use question for geniuses In-Reply-To: References: Message-ID: Hi, All, Electron Microscope Sciences also has a granular formulation of PFA that is not dusty, cat # 12910 , 1kg, --that is what we use ---for those who may need "vast quantities" of 4% PFA as someone recently mentioned. We found it by accident, as there are other vendors that claim their PFA to be granular and yet it is just as dusty as all the others, so I didn't put much stock in their description... but this time, they were right. Nicole Collette Lawrence Livermore National Laboratory/UC Berkeley At 5:16 PM -0500 4/24/08, Johnson, Teri wrote: >Gayle, we can get it from VWR 10 vials of 10 ml per each, Vendor #AA43368-9M > >Our EM dept. gets theirs from Electron Microscopy Sciences, 10 vials >of 10 ml per each, EM Grade, Vendor #15710 > >It's one of the best things I've done for the lab. > >Teri > >-----Original Message----- >From: Gayle Callis [mailto:gayle.callis@bresnan.net] >Sent: Thursday, April 24, 2008 5:02 PM >To: Johnson, Teri; histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] Re: Paraformaldehyde use question for geniuses > > >Dear Teri and Fellow Histonetters, > >Teri's words of wisdom on fixation and storage of samples are well taken. > >Please provide the vendor for the 16% paraformaldehyde in ampules so our >FACS technician doesn't have to weigh out PFA powder anymore. She is going >to be delighted. > >Gayle M. Callis > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rick.Garnhart <@t> memorialhealthsystem.com Fri Apr 25 17:27:50 2008 From: Rick.Garnhart <@t> memorialhealthsystem.com (Rick.Garnhart@memorialhealthsystem.com) Date: Fri Apr 25 17:30:01 2008 Subject: [Histonet] Rick Garnhart/Histology/MEMHOSPCS is out of the office. Message-ID: I will be out of the office starting 04/25/2008 and will not return until 04/29/2008. I will respond to your message when I return. From sbruce <@t> vetpathservicesinc.com Fri Apr 25 17:58:37 2008 From: sbruce <@t> vetpathservicesinc.com (Suzanne Bruce) Date: Fri Apr 25 18:32:38 2008 Subject: [Histonet] Staining Procedures for Technovit 7200 Message-ID: <26DB1FDFBF9EE14AB3AACC873519A4A2514F70@vpss1.VetPathServicesInc.local> Hello! I'm searching for any staining procedures used in Technovit 7200. Anyone willing to share some knowledge? Thanks so much in advance! Suzanne Bruce, R.V.T. Histologist From sherylmccandless <@t> grandecom.net Fri Apr 25 18:45:55 2008 From: sherylmccandless <@t> grandecom.net (Sheryl McCandless) Date: Fri Apr 25 18:46:01 2008 Subject: [Histonet] Re: CE Credits In-Reply-To: <200804250719.m3P7J3o3015788@mxin5.lsn.net> Message-ID: Hi Michelle, I did a lot of the Laboratory Medicine quizzes, attended some of the state meetings, the employer evaluation points, and safety meetings. You need only 2 points in the area of histology and 1 point in safety. If you use the Laboratory Medicine quizzes, you can print off an online transcript directly from the ASCP website and mail that in with your packet. You get some free CE with your ASCP membership and it's a bit less expensive to take the quizzes if you are an ASCP member. You can get 4 points in the three-year period from the employer evaluations (the form is included with your packet). You can also participate in the NSH teleconferences. Each contact hour is worth 1 point, whether it's a teleconference or a safety meeting that your lab has. Just be sure you have the proper documentation in case you are audited. Hope this helps! From JWeems <@t> sjha.org Fri Apr 25 21:24:17 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Apr 25 21:24:24 2008 Subject: [Histonet] Slide File Dividers Message-ID: <982A0A9461F9BF438C7B19A6E425A3830A1631@ITSSSXM01V6.one.ads.che.org> A few months ago we had a discussion about these little paper dividers and I have searched the archieves for everything under the sun (well, almost!) and I cannot find what we may have called them. These are the precut sturdy papers that can be put between every 10 cases or so that help with the confusion of searching. Would you please tell me where the can be purchased and what they are officially called? Thanks much, Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From annigyg <@t> gmail.com Sat Apr 26 07:16:07 2008 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Sat Apr 26 07:16:17 2008 Subject: [Histonet] B plus for BMTs Message-ID: herewith responses to all who asked: its weekend here - will respond re the vendor/supplier from work tomorrow im still at SKMC hospital have not moved Rene - just keeping a low profile jobs scarce - salaries not improving - cleveland (our new bosses) will hopefully make a move to rectify that soon B plus is great - if a tad expensive overnight fixation in NBF works well - but the 'TAT bug' which affects most paths over here forces me to forgo that route - wish i could wave a magic wand and eradicate this incessant need to have quantity over quality it drives me nuts cheers from the desert Annie -- Anne (van Binsbergen) Hope Abu Dhabi UAE From HornHV <@t> archildrens.org Sat Apr 26 09:23:33 2008 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Sat Apr 26 09:24:06 2008 Subject: [Histonet] Slide File Dividers In-Reply-To: <982A0A9461F9BF438C7B19A6E425A3830A1631@ITSSSXM01V6.one.ads.che.org> References: <982A0A9461F9BF438C7B19A6E425A3830A1631@ITSSSXM01V6.one.ads.che.org> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82C09@EMAIL.archildrens.org> Lab Storage Systems. Slide index markers and they also have ones for blocks. www.labstore.com Slide Index Markers You'll never again wonder where a slide has gone... when it's replaced with a Slide Index Marker All the information is at your fingertips: * Case number * Date removed * Initials of person removing slide * 4 vertical spaces for other information, such as locations sent, address, phone number * 3 1/2" high to stand above slides * Easy color coding - available in 6 distinctive colors * Packed in convenient boxes of l,000 markers, one color per box For more information or to place your order call or email us : Toll Free 1.800.345.4167 * Toll Free Fax 1.800.345.4117 * Email Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, April 25, 2008 9:24 PM To: Histonet Subject: [Histonet] Slide File Dividers A few months ago we had a discussion about these little paper dividers and I have searched the archieves for everything under the sun (well, almost!) and I cannot find what we may have called them. These are the precut sturdy papers that can be put between every 10 cases or so that help with the confusion of searching. Would you please tell me where the can be purchased and what they are officially called? Thanks much, Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From annigyg <@t> gmail.com Sat Apr 26 09:33:25 2008 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Sat Apr 26 09:33:35 2008 Subject: [Histonet] pre-cut slide archive markers Message-ID: we make our own from sturdy card - pretty simple actually with a sharp guillotine/cutter -- Anne (van Binsbergen) Hope Abu Dhabi UAE From annigyg <@t> gmail.com Sat Apr 26 09:36:31 2008 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Sat Apr 26 09:36:36 2008 Subject: [Histonet] artisan stainer problems Message-ID: DAKO Artisan: have any of you had hassles with PAS and AB/PAS - bad Schiffs, or Retic - leaky ammonia silver cartridges? general stains feedback also welcome -- Anne (van Binsbergen) Hope Abu Dhabi UAE From Jerry <@t> ralambusa.com Sat Apr 26 12:59:17 2008 From: Jerry <@t> ralambusa.com (Jerry Helisek) Date: Sat Apr 26 12:59:22 2008 Subject: [Histonet] RE: Slide File Dividers Message-ID: <3855F92002259948A66A8CA2D16E3A4F05AFA5@server.ralambusa.com> Joyce, We have those they are product number E43.25, 100 slips per pack, multiple colors available. Please see: http://www.ralamb.net/product_info.php?products_id=82 I hope that helps, if you have any further questions please do not hesitate to contact me by any means possible. ------------------------------------ Raymond A. Lamb, Inc Jerry Helisek VP North America jerry@ralambusa.com 5409 Lumley Road, Unit 102 Durham, North Carolina 27703 tel: 919.957.1964 fax: 919.957.1972 mobile: 919.264.7964 Skype ID:jerryhelisek ------------------------------------ Message: 9 Date: Fri, 25 Apr 2008 22:24:17 -0400 From: "Weems, Joyce" Subject: [Histonet] Slide File Dividers To: "Histonet" Message-ID: <982A0A9461F9BF438C7B19A6E425A3830A1631@ITSSSXM01V6.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" A few months ago we had a discussion about these little paper dividers and I have searched the archieves for everything under the sun (well, almost!) and I cannot find what we may have called them. These are the precut sturdy papers that can be put between every 10 cases or so that help with the confusion of searching. Would you please tell me where the can be purchased and what they are officially called? Thanks much, Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From wood <@t> dcpah.msu.edu Sat Apr 26 23:03:59 2008 From: wood <@t> dcpah.msu.edu (Thomas Wood) Date: Sat Apr 26 23:04:15 2008 Subject: [Histonet] Estrogen receptor for canine Message-ID: Does anyone have a working protocol for IHC that is proven using Estrogen Receptor antibody in canine mammary tissue that they would be willing to share. At one time I had luck with NovoCastra's ER(LH2) but now I only get cytoplasmic staining. -Tom Wood Diagnostic Center for Population and Animal Health Michigan State University From sheila_adey <@t> hotmail.com Sun Apr 27 09:27:36 2008 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Sun Apr 27 09:27:42 2008 Subject: [Histonet] artisan stainer problems In-Reply-To: References: Message-ID: We have the Artisan and have also had a problem with the silver pack leaking, we store the pack upside down to minimize leakage and wipe the tip prior to use. We don't do the PAS on the stainer. Hint: if you do the Warthin Starry on board, charged slides will give lots of back ground. Sheila Adey HT MLTPort Huron HospitalMichigan> Date: Sat, 26 Apr 2008 18:36:31 +0400> From: annigyg@gmail.com> To: histonet@lists.utsouthwestern.edu> Subject: [Histonet] artisan stainer problems> > DAKO Artisan: have any of you had hassles with PAS and AB/PAS - bad Schiffs,> or Retic - leaky ammonia silver cartridges?> general stains feedback also welcome> -- > Anne (van Binsbergen) Hope> Abu Dhabi> UAE> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Find hidden words, unscramble celebrity names, or try the ultimate crossword puzzle with Live Search Games. Play now! http://g.msn.ca/ca55/212 From cbass <@t> wfubmc.edu Sun Apr 27 14:51:20 2008 From: cbass <@t> wfubmc.edu (Caroline Bass) Date: Sun Apr 27 14:51:28 2008 Subject: [Histonet] proper lubricant for an AO 860 Message-ID: <4CCFAF32FB7B2C449A189C82198E42652040B629@EXCHVS1.medctr.ad.wfubmc.edu> Hello Everyone, You may have remembered a post I put up several months ago seeking advice on how to loosen a very gunked up AO 860 sliding microtome. Well, I took it apart bit by bit until I couldn't figure it out anymore. In frustration I left it alone for a while. My boss looked at it recently and with a little WD-40 was able to loosen the mechanism. I'd like to reassemble it (if I can!) but I assume the WD40 needs to be replaced with a better lubricant. Any suggestions? Also, is there anyone out there with experience in taking these things apart? I may need some guidance on how to get it back together. For example, there are these little metal bits in the central metal piece that the blade holder slides down. I think I may have knocked it out of one side. What are these, and are they needed? Finally, I'm in the NC area, can anyone recommend a repair shop for this instrument if I have trouble? Thanks, Caroline From Kemlo.Rogerson <@t> waht.swest.nhs.uk Mon Apr 28 02:55:48 2008 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Apr 28 02:55:54 2008 Subject: [Histonet] proper lubricant for an AO 860 Message-ID: <86ADE4EB583CE64799A9924684A0FBBF03CAE4DF@wahtntex2.waht.swest.nhs.uk> Light machine oil should do, you know the stuff they sell in little cans for around the House, that's what we used. Took a motor bike apart once, never got it back together again, too many bits left over!!!! Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Don't be afraid to take a big step when one is indicated. You can't cross a chasm in two small jumps. --Buckminster Fuller This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From annigyg <@t> gmail.com Mon Apr 28 08:05:07 2008 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Mon Apr 28 08:05:12 2008 Subject: [Histonet] artisan stainer problems In-Reply-To: <1CE1847DFEA0A647B1CCDE4108EA60A747A258@LTA3VS011.ees.hhs.gov> References: <1CE1847DFEA0A647B1CCDE4108EA60A747A258@LTA3VS011.ees.hhs.gov> Message-ID: my paths are complaining about the Artisan PAS - they want it to look like the manual PAS have spent many frustrated hours tweaking and testing, tweaking and testing care to share your protocol? Anne 2008/4/28 Bartlett, Jeanine (CDC/CCID/NCZVED) : > No problems with our PAS...we did have a problem with one stain (I > forget which) only to find out from Dako it was a bad lot but in general > all has been good. > > > Jeanine Bartlett > Infectious Diseases Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anne van > Binsbergen > Sent: Saturday, April 26, 2008 10:37 AM > To: histonet > Subject: [Histonet] artisan stainer problems > > DAKO Artisan: have any of you had hassles with PAS and AB/PAS - bad > Schiffs, or Retic - leaky ammonia silver cartridges? > general stains feedback also welcome > -- > Anne (van Binsbergen) Hope > Abu Dhabi > UAE > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- Anne (van Binsbergen) Hope Abu Dhabi UAE From turkekul <@t> gmail.com Mon Apr 28 11:59:29 2008 From: turkekul <@t> gmail.com (mesruh turkekul) Date: Mon Apr 28 11:59:36 2008 Subject: [Histonet] paraffin mouse femur peroist detaching from slide Message-ID: Dear Friends, I have fixed and decalcified mouse femur with EDTA and paraffin embedded. The sectioning was perfect and also the H&E looked very good. I used super frost/plus slides and baked the sections at 56C 1 hour. After IHC on Ventana machines the periost was gone but the bone marrow was perfectly stable on the sections. Any suggestions? Mesru Turkekul MSKCC.org New York, NY From gayle.callis <@t> bresnan.net Mon Apr 28 12:30:30 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Mon Apr 28 12:30:32 2008 Subject: [Histonet] paraffin mouse femur peroist detaching from slide References: Message-ID: <005f01c8a955$8e7ba000$6501a8c0@DHXTS541> Mesru, Do you mean the dense cortical bone that surrounds the bone marrow? The periosteum is a connective tissue that lines the outside surfaces of cortical bone, next to muscles. You may have over-dried the sections in the oven. A good way to dry decalcified, paraffin embedded bone sections is pick up on superfrost Plus charge slides, drain for a short time in the vertical position, then lay slides FLAT on a 37C to 40C slide warmer, and dry overnight. A 37C oven is fine as long as the slides are flat and no on edge. Metal slide trays help. Longer drying is even better - we let our decalcified bone sections dry for several days. I have one bonehead friend who would not do any staining for two weeks, let her bone sections dry flat that long. 56C may be a bit too hot, and dries out the bone and cartilage even more after the processing., contributing to tissue liftoff. What kind of retrieval did you use? If the retrieval has any kind of mechanical manipulation of the section, then it may fall off e.g. boiling buffers in a microwave. Decalcified bone sections need gentle handling, through deparaffinization, rehydration and all other steps for IHC, in particular any HIER before doing the staining. I am familiar with how the Ventana machine dispenses reagents but if not gentle enough, this could be a mechanical stress on the bone section. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 We have more section lift ----- Original Message ----- From: "mesruh turkekul" To: Sent: Monday, April 28, 2008 10:59 AM Subject: [Histonet] paraffin mouse femur peroist detaching from slide > Dear Friends, > > I have fixed and decalcified mouse femur with EDTA and paraffin embedded. > The sectioning was perfect and also the H&E looked very good. I used super > frost/plus slides and baked the sections at 56C 1 hour. > After IHC on Ventana machines the periost was gone but the bone marrow was > perfectly stable on the sections. > Any suggestions? > > > Mesru Turkekul > > MSKCC.org > New York, NY > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Heather.D.Renko <@t> osfhealthcare.org Mon Apr 28 12:42:01 2008 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Mon Apr 28 12:42:16 2008 Subject: [Histonet] re: histotech posiiton in Rockford, Illinois Message-ID: <40026EDDE64CDA47AB382C52619ACD3C0A09DED3@pmc-rfd-mx01.intranet.osfnet.org> We have a posting for a full time Histotech position in beautiful Rockford, Illinois for an ASCP certified (preferable) Histotech. Please email me for further questions or go online to www.osfhealthcare.org to apply. Heather D. Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 Direct: 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From tjey <@t> hotmail.com Mon Apr 28 13:35:26 2008 From: tjey <@t> hotmail.com (Tanya Ewing-Finchem) Date: Mon Apr 28 13:35:34 2008 Subject: [Histonet] Slide staining rack Message-ID: Hey histo land, I am looking for a slide staining rack (mostly for dehydration and clearing) that holds 10-12 dishes in it and it has one solid lid across that closes and opens all at once. I have seen one before in a catalog, but I can't find it now. Any ideas? TIA Tanya From tjey <@t> hotmail.com Mon Apr 28 13:39:34 2008 From: tjey <@t> hotmail.com (Tanya Ewing-Finchem) Date: Mon Apr 28 13:39:38 2008 Subject: [Histonet] Slide File Dividers In-Reply-To: References: Message-ID: > > Joyce, We use our old business cards, cut them in half long ways and turn them to the back, they work great!!! Tanya> ------------------------------> > Message: 11> Date: Sat, 26 Apr 2008 09:23:33 -0500> From: "Horn, Hazel V" > Subject: RE: [Histonet] Slide File Dividers> To: "Weems, Joyce" , "Histonet"> > Message-ID:> <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82C09@EMAIL.archildrens.org>> Content-Type: text/plain; charset="us-ascii"> > Lab Storage Systems. Slide index markers and they also have ones for> blocks. > > www.labstore.com > From sharon.willman <@t> bms.com Mon Apr 28 13:55:20 2008 From: sharon.willman <@t> bms.com (Sharon E Willman) Date: Mon Apr 28 13:55:30 2008 Subject: [Histonet] Gallyas Stain Message-ID: <48161D98.4030409@bms.com> Hi, Does anyone have a staining protocol for paraffin embedded tissues, using the Gallyas method? I would appreciate any information you might have. Thanks, Sharon From bdelescavage <@t> cellnetix.com Mon Apr 28 16:09:09 2008 From: bdelescavage <@t> cellnetix.com (Beth Delescavage) Date: Mon Apr 28 17:15:14 2008 Subject: [Histonet] HSV I and II controls Message-ID: Hi Everyone~ Does anyone have a good source for HSV I and II controls? If so would you mind sharing, we are having a terrible time finding anything! Thanks in advance for your help!!! Thanks~ Beth Beth Delescavage, BS, HTL (ASCP) QIHC CellNetix Laboratories DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From laurie.colbert <@t> huntingtonhospital.com Mon Apr 28 17:20:11 2008 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Mon Apr 28 17:20:17 2008 Subject: [Histonet] Methods for Freezing Tissue Message-ID: <57BE698966D5C54EAE8612E8941D768302D028C2@EXCHANGE3.huntingtonhospital.com> I am wondering how others are freezing tissue for DNA and/or other studies that needs to be frozen quickly and then held for later use. Currently, we are using a Histobath, that utilizes methylbutane to rapidly freeze tissue. Prior to that, we were using liquid nitrogen. Our Histobath is slowly on it's way out, and is no longer available for purchase from Thermo Fisher (Shandon). Any suggestions?? Laurie Colbert From kmerriam2003 <@t> yahoo.com Tue Apr 29 07:28:56 2008 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Tue Apr 29 07:29:04 2008 Subject: [Histonet] QIHC exam Message-ID: <216181.35609.qm@web50310.mail.re2.yahoo.com> Hi all, I am currently studying for the QIHC exam. As a person that has been working in the biotech/pharma industry for my entire 20+ year career (my, how time flies), I feel that I am at a disadvantage in that I don't do a lot of routine clinical IHC staining; most of my experience has been with animal tissue. Is there a list somewhere that would contain some of the more common clinical IHC stains; I am interested in a list of them and what type of cases that they would be used for. I am not sure if the exam will include questions about specific stains or if it will be mostly based on theory, or a combination of both. Any thoughts/ideas? Kim Merriam, MA, HT(ASCP) Cambridge, MA ____________________________________________________________________________________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ From relia1 <@t> earthlink.net Tue Apr 29 07:39:14 2008 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Apr 29 07:39:18 2008 Subject: [Histonet] RELIA Histology Careers Bulletin 4/29/08 Message-ID: Hi Histonetters!! Spring Has Sprung!! And So Have Some Exciting New Job Opportunities!! I hope you are enjoying a beautiful Spring! Can you believe May Day is Thursday!!! This is a quick update of the positions I am working on that I am most excited about. Some are brand new and some are additional positions where I have placed people who are loving their new jobs. As you know all of the positions I work with are full-time permanent positions with the best hospitals, labs and clinics. I only work with clients that offer excellent compensation including competitive salaries, great benefits and relocation assistance /sign on bonuses. These positions are Day Shift M-F unless noted otherwise. I represent companies nationwide that are in need of histology supervisors histotechnologists and histotechnicians. New Grads Are Welcome To Apply! Here is a list of the positions I am most excited to tell you about: HISTOLOGY MANAGEMENT: Pathology Manager - Portland, OR Histology Manager - Portland, OR Assistant Histology Supervisor - Portland, OR Histology Manager - Palo Alto Area - CA Histology Supervisor - Los Angeles, CA Histology Supervisor - Dallas, Texas HISTOLOGY TECHNICIAN/TECHNOLOGIST Research Histo Tech - Seattle, WA Histo Tech - Spokane, WA Histo Tech - Austin, Texas Histo Tech - Pittsburgh, PA Pathology Asst./Grossing Histo Tech - Mt. Airy, MD Histo Tech - Clearwater, FL Histo Tech - Largo, FL Histo Tech - Tampa, FL Histo Tech - Los Angeles, CA Histo Tech - Helena, MT Histo Tech - Harrisonburg, VA Histo Tech - NYC - NY Histo Tech - Atlanta, GA Histo Tech 3rd shift - Cincinatti, OH If you are interested in any of these positions please call me at 866-607-3542 or e-mail me at relia1@earthlink.net If you would like you can e-mail me your resume and a number where I can reach you at a time that is convenient for you. If you are interested in looking into new job opportunities in other areas that are not mentioned above please contact me as well. I work with facilities nationwide and I will keep your resume confidential. I will only represent you to jobs you tell me you are interested in looking into. Remember. It never hurts to keep an eye open even if you are happy in your present job. Also if you know anyone else that might be interested I would really appreciate it if you would pass my information along to them as well. If you know of anyone who would like to receive my e-mail as well please feel free to send me their contact information and I would be happy to send it to them as well. Thank you, Pam - 866-607-3542 (866-60RELIA) Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.myspace.com/pamatrelia From ksecrest <@t> hsc.wvu.edu Tue Apr 29 08:43:21 2008 From: ksecrest <@t> hsc.wvu.edu (Kimberly Secrest) Date: Tue Apr 29 08:44:17 2008 Subject: [Histonet] CD 26(dipeptidylpeptidase IV) and ICAM1 Message-ID: <4816EDB902000078000032CB@newgwia.hsc.wvu.edu> Hi everyone, I have a researcher wanting to use this antibody on rat muscle tissue. I would really prefer to use paraffin sections as opposed to frozen sections. Has anyone had any success using CD26 on rat tissue in paraffin sections? While I?m asking, how about ICAM1 too? Thanks Kim From spitzern <@t> marshall.edu Tue Apr 29 08:54:19 2008 From: spitzern <@t> marshall.edu (Spitzer, Nadja) Date: Tue Apr 29 08:55:05 2008 Subject: [Histonet] rat brain autofluorescence Message-ID: <16F25B40962D904F81F5502DDD5608A3625F271821@MUXC10.marshall.edu> Hello All, I have spent the last few months troubleshooting my rat brain IHC. I am new to immuno in mammalian tissue and cobbled together a protocol from the literature and extensive reading of the histonet archives. I'm still not very happy with my results and was hoping that you might be able to weigh in with opinions and/or suggestions. I am looking at wtGFP (unfortunately I'm stuck with the wild type) and want to combine it with IHC using the red and blue channels. My major problem has been extreme autofluorescence of the sections in the green channel. I have managed to get rid of the overall green background using a sodium borohydride treatment before antibody application and the sparkly kind of autofluorescence (lipofuscin-like?) with CuSO4 after antibodies. I am quite happy with the reduction in autofluorescence, however, the treatments are pretty hard on my free floating sections and many of them end up looking pretty beat-up. Also, no-one else seems to need these treatments and I wondered if I'm doing something wrong somewhere. The only other explanation I can think of is that the wtGFP is less bright and so I can't turn down the background as much when imaging? Early in my troubleshooting I reduced fixation to perfusion with 400ml of 4%PFA followed by 200ml of 10%sucrose in PBS. The brains go straight into 30%sucrose with no post-fix. I'm having a lot of trouble now, though, with slicing and section integrity so I think I may need to go back to a post-fix, especially since the borohydride and copper seem to work for getting the background down. A lot of my sections are very fragile, sticky and sort of melt-y making me think that I've got insufficient fix. I know I'm going on here so I'll just summarize my protocol and ask you to please comment or suggest possible problems or solutions. Perfuse rat via cardiac puncture; flush with PBS (I was doing 200ml but will reduce this next time to 20-50ml); fix with 400ml PFA; flush with 10%sucrose in PBS; remove brain and place in 30%sucrose refrigerated until it sinks. Freeze brain in 30%sucrose at -80. Cryosection 30um sections at -17degrees into cryoprotectant (Glycerol/Ethylene glycol/PBS); store at -20. For IHC wash in PBS; treat sodium borohydride, wash PBS, preblock with goat serum; primary overnight; wash and then secondary overnight; wash; CuSO4 treatment; wash; mount on superfrost in water and coverslip with Prolong Gold. Thanks very much I appreciate your input. Nadja ~~~~~~~~~~~~~~~~~~~~~~~~ Nadja Spitzer, Ph.D. Cell Differentiation and Development Center Department of Biological Sciences College of Science Marshall University 1 John Marshall Drive Huntington, WV, 25755 email: spitzern@marshall.edu ~~~~~~~~~~~~~~~~~~~~~~~~ From Terri.Brown <@t> Northside.com Tue Apr 29 09:10:39 2008 From: Terri.Brown <@t> Northside.com (Terri Brown) Date: Tue Apr 29 09:11:07 2008 Subject: [Histonet] HEIR2Neu Message-ID: <8CEB6DA1A3F35743800669D4CFE21F7D03F8FF00@NSMXMS04.northside.local> Hi, How are you tracking the fixation time for breast tissue from the time the specimen is put into formalin to the time it comes off the processors? Do you have the time recorded on a data sheet for CAP inspectors to review or on your req. and if so is this time dictated by the PA at the time of grossing? Thanks in advance for your help. Terri H. Brown Pathology Laboratory Manager Northside Hospital Office: 404-845-5423 Fax: 404-851-6400 terri.brown@northside.com CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. From rmweber113 <@t> comcast.net Tue Apr 29 09:11:35 2008 From: rmweber113 <@t> comcast.net (rmweber113@comcast.net) Date: Tue Apr 29 09:11:45 2008 Subject: [Histonet] JOB OFFER Message-ID: <042920081411.19165.48172C970007E8D000004ADD2216554886CCCECE9D0A0D0A99039D@comcast.net> I have a full time job offer for a ASCP certified histologist at a new state of the art laboratory. This is for a privately owned prestigious GI group in Wayne NJ. Candidate should possess the skills to set up, maintain, and develop a successful histology laboratory. Candidate will be proficient in grossing and all phases of routine histology. We offer a $500 referral bonus to anyone who refers a candidate whom we hire and stays with us for at least 90 days. Interested candidates can call me at 732 814-1170 for more information or email me their resumes. Thank you. From gayle.callis <@t> bresnan.net Tue Apr 29 10:46:30 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Tue Apr 29 10:46:31 2008 Subject: [Histonet] rat brain autofluorescence References: <16F25B40962D904F81F5502DDD5608A3625F271821@MUXC10.marshall.edu> Message-ID: <004c01c8aa10$31d747d0$6501a8c0@DHXTS541> It sounds as though your fixation is very poor per your sticky, melty comment. If you flush the brain with cryoprotectant right after the fixative you probably not allowing the PFA to crosslink the antigens with sufficient time, and fixative is washed out by the cryoprotectant. We postfix brain of hamsters/mice after perfusion by immersion in the fixative for additional 5 to 6 hours, maybe more would be better for you. There are those who can guide you through rat brain fixation. The cryoprotection can be done at 4C by immersing the brain overnight or longer AFTER the fixation is done, not as a perfusion method. Timing on cryoprotection of a rat brain should be forthcoming from those working with this species. I suggest you access this free booklet in pdf form from Clontech/BD Bioscience website. Just Google Living Colors. ?. User Manual (PT2040-1) www.clontech.com/images/pt/dis_vectors/PT3598-5.pdf . There are excellent troubleshooting guides for wtGFP, eGFP and other GFP chimeras. There is a discussion of the autofluorescence patterns you will see with GFP in tissues/cells and staining protocols. Also, there are suggestions on how to reduce autofluorescence on the IHCWorld website other than what you use plus a summary of methods written up by a gentleman, found in Histonet Archives. If you want a review of autofluorescence (privately attached) pertains directly to GFP but also applicable to other fluorophores, I will be happy to send it to you. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 More results from www.clontech.com ? ----- Original Message ----- From: "Spitzer, Nadja" To: Sent: Tuesday, April 29, 2008 7:54 AM Subject: [Histonet] rat brain autofluorescence Hello All, I have spent the last few months troubleshooting my rat brain IHC. I am new to immuno in mammalian tissue and cobbled together a protocol from the literature and extensive reading of the histonet archives. I'm still not very happy with my results and was hoping that you might be able to weigh in with opinions and/or suggestions. I am looking at wtGFP (unfortunately I'm stuck with the wild type) and want to combine it with IHC using the red and blue channels. My major problem has been extreme autofluorescence of the sections in the green channel. I have managed to get rid of the overall green background using a sodium borohydride treatment before antibody application and the sparkly kind of autofluorescence (lipofuscin-like?) with CuSO4 after antibodies. I am quite happy with the reduction in autofluorescence, however, the treatments are pretty hard on my free floating sections and many of them end up looking pretty beat-up. Also, no-one else seems to need these treatments and I wondered if I'm doing something wrong somewhere. The only other explanation I can think of is that the wtGFP is less bright and so I can't turn down the background as much when imaging? Early in my troubleshooting I reduced fixation to perfusion with 400ml of 4%PFA followed by 200ml of 10%sucrose in PBS. The brains go straight into 30%sucrose with no post-fix. I'm having a lot of trouble now, though, with slicing and section integrity so I think I may need to go back to a post-fix, especially since the borohydride and copper seem to work for getting the background down. A lot of my sections are very fragile, sticky and sort of melt-y making me think that I've got insufficient fix. I know I'm going on here so I'll just summarize my protocol and ask you to please comment or suggest possible problems or solutions. Perfuse rat via cardiac puncture; flush with PBS (I was doing 200ml but will reduce this next time to 20-50ml); fix with 400ml PFA; flush with 10%sucrose in PBS; remove brain and place in 30%sucrose refrigerated until it sinks. Freeze brain in 30%sucrose at -80. Cryosection 30um sections at -17degrees into cryoprotectant (Glycerol/Ethylene glycol/PBS); store at -20. For IHC wash in PBS; treat sodium borohydride, wash PBS, preblock with goat serum; primary overnight; wash and then secondary overnight; wash; CuSO4 treatment; wash; mount on superfrost in water and coverslip with Prolong Gold. Thanks very much I appreciate your input. Nadja ~~~~~~~~~~~~~~~~~~~~~~~~ Nadja Spitzer, Ph.D. Cell Differentiation and Development Center Department of Biological Sciences College of Science Marshall University 1 John Marshall Drive Huntington, WV, 25755 email: spitzern@marshall.edu ~~~~~~~~~~~~~~~~~~~~~~~~ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcline <@t> wchsys.org Tue Apr 29 11:00:21 2008 From: jcline <@t> wchsys.org (Joyce Cline) Date: Tue Apr 29 11:00:28 2008 Subject: [Histonet] validation of lot #'s for IHC & Specials Message-ID: <000601c8aa12$20f3f010$1d2a14ac@wchsys.org> We currently pull a control already stained with the old lot # and stain a control with the new lot # and compare staining. Our paths are saying you should use the same control block for both the old and new lot #'s but we don't always have the same block (might have been exhausted) Does anyone keep a control block without exhausting it, for lot validation? Does anyone check lot #'s for their special stains. We have a Ventana NexES Sp. Stainer and we order pre-made kits for our hand staining. Our paths want us to validate the lot #'s for our specials also. Thanks..Joyce :-) ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From LSebree <@t> uwhealth.org Tue Apr 29 11:13:39 2008 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Tue Apr 29 11:13:47 2008 Subject: [Histonet] validation of lot #'s for IHC & Specials In-Reply-To: <000601c8aa12$20f3f010$1d2a14ac@wchsys.org> Message-ID: Joyce, We pretty much do something similar as you for IHC control lot verification. If we've exhausted a block currently in use as a control, we'll select a new tissue and stain it with the old lot and new lot. Obviously this takes some planning ahead but you'd never want to run out of your old lot of antibody before you have some new on hand. We've just moved to a new system whereas we maintain 1 soft tissue control and 1 bm bx control (if applicable) for routine use. However we have on hand several control tissues to check out new lots of antibody. We feel this is a "safer" method of lot verification using controls of varying degrees of immunoreactivity. I hope this isn't too confusing. If you have further questions, feel free to contact us. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Tuesday, April 29, 2008 11:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] validation of lot #'s for IHC & Specials We currently pull a control already stained with the old lot # and stain a control with the new lot # and compare staining. Our paths are saying you should use the same control block for both the old and new lot #'s but we don't always have the same block (might have been exhausted) Does anyone keep a control block without exhausting it, for lot validation? Does anyone check lot #'s for their special stains. We have a Ventana NexES Sp. Stainer and we order pre-made kits for our hand staining. Our paths want us to validate the lot #'s for our specials also. Thanks..Joyce :-) ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Xorren <@t> aol.com Tue Apr 29 11:43:10 2008 From: Xorren <@t> aol.com (Xorren@aol.com) Date: Tue Apr 29 11:43:26 2008 Subject: [Histonet] Job postitions in NY area Message-ID: Hello all Histonetters ! Does anyone know of any postions for a registered HT, Cytotechnician or general laboratory support personell in the NYC or Long Island area? If so, please e-mail me @ Xorren @aol.com Thanks so much to all ! ************** Need a new ride? Check out the largest site for U.S. used car listings at AOL Autos. (http://autos.aol.com/used?NCID=aolcmp00300000002851) From strogen <@t> optonline.net Tue Apr 29 12:00:55 2008 From: strogen <@t> optonline.net (Robin Strogen) Date: Tue Apr 29 12:02:01 2008 Subject: [Histonet] unsubscribe Message-ID: <0K03007OCJB64EH0@mta5.srv.hcvlny.cv.net> unsubscribe From Heather.D.Renko <@t> osfhealthcare.org Tue Apr 29 12:11:12 2008 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Tue Apr 29 12:11:29 2008 Subject: [Histonet] Re: tracking fixation times for breast cases Message-ID: <40026EDDE64CDA47AB382C52619ACD3C06612DEA@pmc-rfd-mx01.intranet.osfnet.org> Teri, We have a punch clock that is used for our frozen section req. that we use when the specimen comes in the door and is put into formalin. The pathologists then dictates this time into the report and states how long the tissue is/will be in formalin before the processor will start. For the inspectors, CAP can then pull any breast case report up electronically to validate the phase 1 recommendation is being adhered to for HER2neu specimens. How are you tracking the fixation time for breast tissue from the time the specimen is put into formalin to the time it comes off the processors? Do you have the time recorded on a data sheet for CAP inspectors to review or on your req. and if so is this time dictated by the PA at the time of grossing? Heather D. Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 Direct: 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From RSRICHMOND <@t> aol.com Tue Apr 29 12:55:35 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Tue Apr 29 12:55:45 2008 Subject: [Histonet] Re: magnifier for grossing Message-ID: Traditionally one does not use magnification for gross examination of surgical specimens, but as eyes get older and biopsy specimens get smaller, it's increasingly difficult to do without it. Surgeons, of course, use magnification when operating on small lesions, and are occasionally surprised that pathologists do not use it. I've been using a binocular magnifier mounted on a headband that includes a swivel that moves it up out of the way when it isn't needed. Called an OptiVISOR, it's made by Donegan Optical Company, located in Lenexa, Kansas, wherever that is. See www.doneganoptical.com for a picture and a clear explanation of how it works. I have the #4 lens plate, with a 10 inch focal length. I think the slightly weaker #3 lens plate, with a 14 inch focal length, might be more practical, particularly if you have to stand up (rather than sit down) to gross, as is also the tradition. I would think that the OptiVISOR would also be useful to histotechs over a certain age who do paraffin embedding, though the ones I've offered to lend mine to have been too set in their ways to consider a change. I bought my magnifier at a gem and mineral show in 1994. Presently they cost about US$40 including shipping. Donegan Optical doesn't sell retail, but you can Google optivisor to find vendors. Amazon has them. I have no affiliation with Donegan or Amazon. Bob Richmond Samurai Pathologist Knoxville TN From Shirley_PHUA <@t> hsa.gov.sg Tue Apr 29 13:03:33 2008 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Tue Apr 29 13:06:37 2008 Subject: [Histonet] Shirley Phua is out-of-office ... Message-ID: I will be out of the office from 29-04-2008 to 30-04-2008. I'll be away on 30 April 2008. Pathologists: I will process your requests when I return. If urgent, please forward your email to Henry_Kyaw@hsa.gov.sg From making <@t> ufl.edu Tue Apr 29 13:15:25 2008 From: making <@t> ufl.edu (MKing) Date: Tue Apr 29 13:19:58 2008 Subject: [Histonet] rat brain autofluorescence Message-ID: <481765BD.906@ufl.edu> Nadja, You might want to try Sudan Black as an alternative to borohydride and CuSO4. You can find an appropriate and reproducible suppression of autofluor by testing a dilution series starting with 0.1% in 70% ethanol (Journal of Histochemistry and Cytochemistry, Vol. 49, 1565-1572, Control of Autofluorescence of Archival Formaldehyde-fixed, Paraffin-embedded Tissue in Confocal Laser Scanning Microscopy (CLSM),Werner Baschong, Rosmarie Suetterlin, and R. Hubert Laeng). I've had pretty good luck with this on free-floating rat brain sections and GFP, usually with dilutions of the 0.1% by 10-100X. If you start with 100X diluted 0.1% and it isn't enough you can put the section back in a higher concentration until its right. Kind of a nice counterstain for showing tissue structure (fiber tracts) too. For postfix we refrigerate the whole perfused (100 ml wash, 400 ml fix) rat 2 hr. before removing the brain into 30% sucrose PBS. It gives the fixative a bit more time to work without impairing immunolabeling for most antibodies. The glycerol/glycol may be responsible for some of the gooeyness of your sections, too. Good luck, Mike King UF Pharmacology & Therepeutics ------------------------------ Date: Tue, 29 Apr 2008 09:54:19 -0400 From: "Spitzer, Nadja" Subject: [Histonet] rat brain autofluorescence Hello All, ...extreme autofluorescence of the sections...the treatments are pretty hard on my free floating sections... Thanks very much I appreciate your input. Nadja From Dave.Pizi <@t> carolinashealthcare.org Tue Apr 29 13:43:43 2008 From: Dave.Pizi <@t> carolinashealthcare.org (Pizi, Dave) Date: Tue Apr 29 13:44:13 2008 Subject: [Histonet] Job openings Message-ID: <7E22BEDC45D51449B847E15983E99BAD08185287@dcr-xchg-04.Carolinas.org> Carolinas HealthCare System in Charlotte, NC has two full time positions available. These positions qualify for a sign-on bonus and relocation assistance. If interested, please see the April 7 issue of Advance or call 800-541-4354. David Pizi Histology Supervisor ----------------------------------------- This electronic message may contain information that is confidential and/or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you. From fong <@t> zoology.ubc.ca Tue Apr 29 14:01:09 2008 From: fong <@t> zoology.ubc.ca (Angelina Fong) Date: Tue Apr 29 14:01:18 2008 Subject: [Histonet] HRP travel times and speed Message-ID: <48177075.5020003@zoology.ubc.ca> Hi histonetters, Does anyone know what the rate / speed of transport for HRP is? Does anyone know a reference that has looked at or reports the rate of transport of some common tracers like HRP or WGA? Thanks!! Cheers Angelina -- ~~o~~o~~o~~o~~o~~o~~o~~o~~o~~o Angelina Y. Fong, Ph.D. Department of Zoology Biological Sciences Building 6270 University Boulevard University of British Columbia Vancouver, BC, V6T 1Z4 Canada Ph: (604) 822-5990 Fax: (604) 822-2416 Email: fong@zoology.ubc.ca From hodges420 <@t> msn.com Tue Apr 29 14:02:21 2008 From: hodges420 <@t> msn.com (MARY T HODGES) Date: Tue Apr 29 14:02:33 2008 Subject: [Histonet] tissue processor auto dial up Message-ID: Good day all, Can any one offer information on how and where a auto dial up can be purchased for a tissue processor. We bought a new processor and the company is not very helpful as to where we can purchase the alarm and how to do so. Any input would be appreciated. Thanks, Tere Hodges _________________________________________________________________ Make i'm yours.? Create a custom banner to support your cause. http://im.live.com/Messenger/IM/Contribute/Default.aspx?source=TXT_TAGHM_MSN_Make_IM_Yours From sbruce <@t> vetpathservicesinc.com Tue Apr 29 14:01:33 2008 From: sbruce <@t> vetpathservicesinc.com (Suzanne Bruce) Date: Tue Apr 29 14:04:01 2008 Subject: FW: [Histonet] Staining Procedures for Technovit 7200 References: <26DB1FDFBF9EE14AB3AACC873519A4A2514F70@vpss1.VetPathServicesInc.local> <002201c8a7ae$b5e43880$6501a8c0@DHXTS541> Message-ID: <26DB1FDFBF9EE14AB3AACC873519A4A2514F80@vpss1.VetPathServicesInc.local> Thanks Gayle, I am searching for a VVG or any other special stains. I think I have found a VVG published earlier in the archives. Suzanne Bruce, R.V.T. Histologist ________________________________ From: Gayle Callis [mailto:gayle.callis@bresnan.net] Sent: Sat 4/26/2008 11:03 AM To: Suzanne Bruce Subject: Re: [Histonet] Staining Procedures for Technovit 7200 Technovit it GMA. If you do a search for glycol methacrylate in Histonet website, you will find methods. If you tell us what stains you want to do, it will help. Normally, any stain that is used for paraffin can be used on GMA sections, but you do NOT have to run down to water, as the plastic is in the tissue forever, it cannot be removed and if you want to do immunostaining, it is not a good embedding media for that as immunoglobulins cannot penetrate the plastic matrix to get to the antigens. Gayle M. Callis HTl/HT/MT(ASCP) ----- Original Message ----- From: "Suzanne Bruce" To: "histonet" Sent: Friday, April 25, 2008 4:58 PM Subject: [Histonet] Staining Procedures for Technovit 7200 Hello! I'm searching for any staining procedures used in Technovit 7200. Anyone willing to share some knowledge? Thanks so much in advance! Suzanne Bruce, R.V.T. Histologist Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From KPierce <@t> cancer-test.com Tue Apr 29 14:26:13 2008 From: KPierce <@t> cancer-test.com (Ken Pierce) Date: Tue Apr 29 14:26:32 2008 Subject: [Histonet] histology position Message-ID: <9380B79A09A6DD43884D977256FC12020F5293@fleming.mla.local> Medical Lab Assoc. in Seattle WA will have a histo position available in May. Hours are 6 AM to 2 PM, Monday through Friday. Competitive pay, health benifits. Small lab, experience in IHC welcomed. Contact Ken Pierce, Lab Supervisor, Medical Lab Associates, 206-623-3814 or e-mail kpierce@cancer-test.com. From melissa.mazan <@t> tufts.edu Tue Apr 29 14:34:27 2008 From: melissa.mazan <@t> tufts.edu (Melissa Mazan) Date: Tue Apr 29 14:34:36 2008 Subject: [Histonet] frozen sections - lung In-Reply-To: <200804291709.m3TH9BfK018695@mail-proofpoint-2a.usg.tufts.edu> References: <200804291709.m3TH9BfK018695@mail-proofpoint-2a.usg.tufts.edu> Message-ID: <48177843.7050307@tufts.edu> Hi all, I am writing to request help with frozen sections. We do most of our work in FFPE tissues and are just starting on frozens. I'm using a protocol posted, I believe, by Gayle Callis a while ago. Briefly, we anesthetize the mouse, free the trachea of surrounding tissues, place a 20 g catheter through the trachea, then open the chest and free the lung tissue, and transect the abdominal aorta. We perfuse through the left side of the heart first with around 10 mls of PBS, then with 10 mls of 4% paraformaldehyde. This usually turns the lungs white. Then, we fill the lungs with one vital capacity of OCT, clamp the trachea, and carefully dissect the lungs free - then either snap freeze the whole lung or lung lobes in OCT in a tissue tek cassette, and store at -80. I then make slides - about 10 microns thick - on star plus slides - and let air dry at least one hour, or overnight, then post fix them either in acetone at -20 C for 10 minutes, or in paraformaldehyde for 10 minutes. The tissues look very nice on H and E - good alveolar and airway structure, quite nice quality cells and scaffolding. However, my immunostaining is NOT coming out as well as it usually does on paraffin sections. Do you make modifications to your protocols for paraffin? (usually permeabilize with either TBST or PBS-Tx, block for one hour, primary diluted in same buffer for either 1 hour at RT or overnight at 4C, then washed, secondary for 1/2 hour at 37C). And how do you store the slides once you have made them? Can they stay at RT or must they be frozen? Many thanks - Melissa > ************** > From laurie.colbert <@t> huntingtonhospital.com Tue Apr 29 16:39:53 2008 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Tue Apr 29 16:39:57 2008 Subject: [Histonet] Surgipath Rep Message-ID: <57BE698966D5C54EAE8612E8941D768302D02AB6@EXCHANGE3.huntingtonhospital.com> Is there a Surgipath rep for southern California (Pasadena) that would contact me? Laurie Colbert Huntington Hospital From ks_andthe4foots <@t> yahoo.com Tue Apr 29 17:22:26 2008 From: ks_andthe4foots <@t> yahoo.com (Steven Caokley) Date: Tue Apr 29 17:22:30 2008 Subject: [Histonet] unsubcribe Message-ID: <200446.86293.qm@web45716.mail.sp1.yahoo.com> unsubcribe _________________________________________________________________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. [1]Try it now. References 1. http://us.rd.yahoo.com/evt=51733/*http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ From alzalex <@t> aol.com Tue Apr 29 18:23:41 2008 From: alzalex <@t> aol.com (alzalex@aol.com) Date: Tue Apr 29 18:23:53 2008 Subject: [Histonet] Re: remove from list Message-ID: <8CA786731E64123-1408-244B@FWM-M20.sysops.aol.com> Please remove me from the mailing list Thank you Anita Zuniga From jnocito <@t> satx.rr.com Tue Apr 29 18:25:45 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Apr 29 18:25:50 2008 Subject: [Histonet] validation of lot #'s for IHC & Specials References: <000601c8aa12$20f3f010$1d2a14ac@wchsys.org> Message-ID: <002b01c8aa50$5a209710$0302a8c0@yourxhtr8hvc4p> Joyce, no and yes. We go through so many controls that we often exhaust the block, especially on the scarce controls like AFB, AFP, ADV. We have the Ventana stainers also and verify the different lot numbers also. JTT ----- Original Message ----- From: "Joyce Cline" To: Sent: Tuesday, April 29, 2008 11:00 AM Subject: [Histonet] validation of lot #'s for IHC & Specials > We currently pull a control already stained with the old lot # and stain > a control with the new lot # and compare staining. Our paths are saying > you should use the same control block for both the old and new lot #'s > but we don't always have the same block (might have been exhausted) > > Does anyone keep a control block without exhausting it, for lot > validation? > Does anyone check lot #'s for their special stains. We have a Ventana > NexES Sp. Stainer and we order pre-made kits for our hand staining. Our > paths want us to validate the lot #'s for our specials also. > > Thanks..Joyce :-) > > > ***** CONFIDENTIALITY NOTICE ***** > This message contains confidential information and is intended only for > the individual named. If you are not the named addressee you should not > disseminate, distribute or copy this e-mail. Please notify the sender > immediately by e-mail if you have received this e-mail by mistake and > delete this e-mail from your system. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From annabelmazza <@t> yahoo.com Tue Apr 29 21:25:22 2008 From: annabelmazza <@t> yahoo.com (Annabel L. Mazza) Date: Tue Apr 29 21:25:33 2008 Subject: [Histonet] certification-salary/temp positions Message-ID: <502173.20114.qm@web35707.mail.mud.yahoo.com> Hi!!!,Histonetters. I want to congratulate you all for to create this wonderful,wonderful forum, I been visiting this site since 2005 to read yours articles, recommendations, references, procedures etc, an again I think that is a wonderful forum. Is the second, third time or maybe more that I read topics as a Certifications, Salary, To be eligible for HT exam etc and I want to tell my history. I started off as a histology assistant helping Doctors in the Gross Room as a teaching Hospital helping Pathology Residents to do grossing, changing solutions on the tissue processor's, staining machines, organize the slides cases on the folders to be diagnose. Beside that at third year I was trained on the Autopsies including brains & Spinal Cords, also take training in Cytology, accessioning cases, processing like Cytospin and Cell Blocks on Non-Gyn cases. After six years I got a contract in Miami VA, another teaching institution under University of Miami with triple autopsy cases, again to attend Professors and training Residents on full Autopsies with skills on brain cuttings working pretty close to the Neuropathology Attendant. Then I started off the whole processing with autopsy tissues, fixation, processing, embedding, cutting(brains includes) H&E stain and special stains so I became in a regular tech, embedding, cutting... .In Cytology processing I do fluids, sputum(Non-Gyn's) & Gyn's in the THIN PREP machine, also FNA's processing etc. I was pretty lucky to be in histology labs in such a big Institutions. During those years I tried twenty four credicts, biology OK, Chemistry OK, but the Algebra II with trigonometry make me give up. After three years contract at VA Hosp. I left the Histology and I'm doing another thing related with Laboratories but I gave up to get any License or Degree in Histology I just have a High School Diploma from my country(Cuba). Now I'm 42 y/o, divorced with teens and telling you all the true I like to do histology, autopsies included, but "Se la Vid". THANKS for let me to publish my short history as histotech. Juan Pena-Torres PS. This Article may appear under Annabel Mazza, she's my fiance, but GO HISTONETERS!!! magnificent site. Annabel bel ____________________________________________________________________________________ Yahoo! Deportes Beta ?No te pierdas lo ?ltimo sobre el torneo clausura 2008! Ent?rate aqu? http://deportes.yahoo.com From fieldsfamily15 <@t> bellsouth.net Tue Apr 29 22:24:25 2008 From: fieldsfamily15 <@t> bellsouth.net (fieldsfamily15@bellsouth.net) Date: Tue Apr 29 22:24:30 2008 Subject: [Histonet] Her2 fixation tracking Message-ID: <043020080324.27848.4817E669000A1B0900006CC822230650029B0A02D2089B9A019C04040A0DBFCACE970407030E009C0B040A0700@att.net> Hi Histo techs, Could you please let me know how you are handling tracking the time your specimens for Her 2 go into formalin? I need to know if you have the doctors put the time the tissue goes into formalin on the container or it is on a log or what... I am trying to see what solutions are working for you. I need to make sure the time in fixation plus the time on the processors is within the CAP recommended range. We are sending the Her2's out for the immunos but we have to document the total fixation time. Hope this makes sense...and thank you for any help. Carole Fields Northside Hospital Atlanta, GA From jkiernan <@t> uwo.ca Tue Apr 29 23:57:49 2008 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Tue Apr 29 23:57:54 2008 Subject: [Histonet] Gallyas Stain Message-ID: There is no single Gallyas method. Ferenc Gallyas published several silver staining methods for paraffin embedded tissues, especially CNS. They have in common: accurate control of silver and silver-diammine ion concentrations (by using ammonium nitrate and NaOH rather than ammonium hydroxide), and stabilization of the physical developer with tungstosilicic (= silicotungstic) acid. Specificity for such things as microglia, neurofibrillary tangles, capillaries and immunohistochemical reaction (DAB) products is determined by pre-treatments. Don't do a Gallyas method on the strength of someone's typed worksheet or an internet posting. Get your instructions from a primary source. The following references may be helpful. This list does not include all Gallyas' papers, only the ones that I have read and found interesting enough to record. Gallyas (1963) Silver microglia method. Acta Neuropathol. (Berl.) 3: 206-209. Gallyas F (1979) Light insensitive physical developers. Stain Technol. 54: 173-176. Gallyas F (1980) Chemical nature of the first products (nuclei) of the argyrophil staining. Acta Histochem. 67: 145-158. Gallyas F, Gorcs T, Merchenthaler I (1982) High-grade intensification of the end-product of the diaminobenzidine reaction for peroxidase. J. Histochem. Cytochem. 30: 183-184. Gallyas F, Wolff JR (1986) Metal-catalysed oxidation renders silver intensification selective. Applications for the histochemistry of diaminobenzidine and neurofibrillary changes. J. Histochem. Cytochem. 34: 1667-1672. Gallyas F (1971) Silver staining of Alzheimer's neurofibrillary changes by means of physical development. Acta Morphol. Acad. Sci. Hung. 19: 1-8. Gallyas F (1971) A principle for silver staining of tissue elements by physical development. Acta Morphol. Acad. Sci. Hung. 19: 57-71. Merchenthaler I, Stankovics J, Gallyas F (1989) A highly sensitive one-step method for silver intensification of the nickel-diaminobenzidine endproduct of peroxidase reaction. J. Histochem. Cytochem. 37: 1563-1565. Gallyas F, Guldner FH, Zoltay G, Wolff JR (1990) Golgi-like demonstration of 'dark' neurons with an argyrophil III method for experimental neuropathology. Acta Neuropathol. (Berl.) 79: 620-628. Gallyas F, Zoltay G (1992) An immediate light microscopic response of neuronal somata, dendrites and axons to non-contusing concussive head injury in the rat. Acta Neuropathol. (Berl.) 83: 386-393. Gallyas F, Zoltay G, Balas R (1992) An immediate light microscopic response of neuronal somata, dendrites and axons to contusing concussive head injury in the rat. Acta Neuropathol. (Berl.) 83: 394-401. Gallyas F, Zoltay G, Horvath Z (1992) Light microscopic response of neuronal somata, dendrites and axons to post-mortem concussive head injury. Acta Neuropathol. (Berl.) 83: 499-503. Gallyas F, Zoltay G, Dames W (1992) Formation of 'dark' (argyrophilic) neurons of various origin proceeds with a common mechanism of biophysical nature (a novel hypothesis). Acta Neuropathol. (Berl.) 83: 504-509. Gallyas F, Hsu M, Buzsaki G (1993) Four modified silver methods for thick sections of formaldehyde-fixed mammalian central nervous tissue: dark neurons, perikarya of all neurons, microglial cells and capillaries. J. Neurosci. Methods 50: 159-164. Gallyas F (1982) Physicochemical mechanism of the argyrophil I reaction. Histochemistry 74: 393-407. Gallyas F (1982) Physicochemical mechanism of the argyrophil III reaction. Histochemistry 74: 409-421. Gallyas F, Merchenthaler I (1988) Copper-H2O2 oxidation strikingly improves silver intensification of the nickel-diaminobenzidine (Ni-DAB) end-product of the peroxidase reaction. J. Histochem. Cytochem. 36: 807-810. Some of Gallyas' methods have been improved upon by others, including Braak and Munoz in the context of pathology of Alzheimer's disease and other dementias. These and related methods were reviewed by Bill Grizzle (1996) Theory and practice of silver staining in histopathology. J. Histotechnol. 19: 183-195. This is in a special issue of the Journal of Histotechnology (Vol 19#3) devoted to silver staining. Read it. You may find a technically simpler but adequate alternative to the Gallyas method you were asking about. Gallyas' techniques are for the "advanced" worker (technician or academic) who understands silver staining techniques. They won't work in the hands of a new graduate student or a molecular biology postdoc with no prior instruction or experience in using methods of this kind. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Sharon E Willman Date: Monday, April 28, 2008 14:56 Subject: [Histonet] Gallyas Stain To: histonet@lists.utsouthwestern.edu > Hi, > Does anyone have a staining protocol for paraffin embedded > tissues, > using the Gallyas method? I would appreciate any > information you might > have. > Thanks, > Sharon > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aa2 <@t> sun.ac.za Wed Apr 30 01:54:10 2008 From: aa2 <@t> sun.ac.za (Alblas, Mandi ) Date: Wed Apr 30 01:54:19 2008 Subject: [Histonet] Sectioning of gut content Message-ID: <87F01929E7BA354F8C203AE22D267A8D03F0DCF8@TYGEVS01.tyg.sun.ac.za> Hi all! I am trying to section ingesta containing sand, but battle to get a decent cut. Is there any advice on how to get the sand in such way to be able to cut it with a normal disposable blade? There were a suggestion that I should try with a tungsten blade, but I could not get a supplier yet to provide one. Any suggestions? (PS we can not remove/wash out the sand, its needed for analysis). Thanks Mandi Alblas Stellenbosch University From pruegg <@t> ihctech.net Wed Apr 30 07:10:48 2008 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Wed Apr 30 07:03:03 2008 Subject: [Histonet] Sectioning of gut content In-Reply-To: <87F01929E7BA354F8C203AE22D267A8D03F0DCF8@TYGEVS01.tyg.sun.ac.za> Message-ID: <200804301202.m3UC2qLf043951@pro42.abac.com> Sounds like a tape transfer system like the Instrumedics now from McCormick is what you need. The tape on the block will keep the section together and then you roll the tape out on a polymer coated slide, expose the slide to UV and it polymerizes the section from the tape to the slide so that you can then remove the tape. You might have trouble getting the tape off and leaving all the section behind because the gritty sand may not lay completely flat but this is probably your best bet. Another possibility I thought of was trying to use a vibrotome to section this but the sections are 30+ microns thick???? Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alblas, Mandi Sent: Wednesday, April 30, 2008 12:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sectioning of gut content Hi all! I am trying to section ingesta containing sand, but battle to get a decent cut. Is there any advice on how to get the sand in such way to be able to cut it with a normal disposable blade? There were a suggestion that I should try with a tungsten blade, but I could not get a supplier yet to provide one. Any suggestions? (PS we can not remove/wash out the sand, its needed for analysis). Thanks Mandi Alblas Stellenbosch University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PFlowers <@t> seton.org Wed Apr 30 08:57:04 2008 From: PFlowers <@t> seton.org (Flowers, Penny) Date: Wed Apr 30 08:59:05 2008 Subject: [Histonet] Penfix vs Immunohistochemistry Message-ID: <19A903F72A2F5E4785B582C6DBF70E0D0218C587@AUSEX2VS1.seton.org> We had an article some years back that stated that pen-fix interferes with IHC, especially for Her2neu. What are others doing. Are you using Pen-fix on your processors with breasts, etc and fatty tissues? Thanks, Penny From lcates <@t> synecor.com Wed Apr 30 09:03:24 2008 From: lcates <@t> synecor.com (Lynne Cates) Date: Wed Apr 30 09:03:31 2008 Subject: [Histonet] Clip on Tags Message-ID: I need help please! I am looking for a clip on tag, paper or metal that can be labeled and placed on a sample at necropsy. Thanks so much. Lynne Cates, HT (ASCP) From eca9 <@t> georgetown.edu Wed Apr 30 09:11:23 2008 From: eca9 <@t> georgetown.edu (Eva CA Permaul) Date: Wed Apr 30 09:11:35 2008 Subject: [Histonet] TGF staining? Message-ID: <11b46611ad2e.11ad2e11b466@imap.georgetown.edu> Good morning out there is histoland, I am interested in doing some TGFb 1,2 and 3 as well as TGFbR IHC staining. It will be used to stain Human Breast tissue. Could someone recommend any particular antibody? Thanks, Eva Georgetown University From settembr <@t> umdnj.edu Wed Apr 30 08:59:56 2008 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Wed Apr 30 09:18:37 2008 Subject: [Histonet] Thymidylate Phosphostase Message-ID: Hello, I am looking for Thymidylate Phosphotase for use on formalin fixed paraffin embedded human tissue. Is anyone using this on paraffin? What vendor do you use? Thank you, Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Joe Nocito 04/29/08 7:25 PM >>> Joyce, no and yes. We go through so many controls that we often exhaust the block, especially on the scarce controls like AFB, AFP, ADV. We have the Ventana stainers also and verify the different lot numbers also. JTT ----- Original Message ----- From: "Joyce Cline" To: Sent: Tuesday, April 29, 2008 11:00 AM Subject: [Histonet] validation of lot #'s for IHC & Specials > We currently pull a control already stained with the old lot # and stain > a control with the new lot # and compare staining. Our paths are saying > you should use the same control block for both the old and new lot #'s > but we don't always have the same block (might have been exhausted) > > Does anyone keep a control block without exhausting it, for lot > validation? > Does anyone check lot #'s for their special stains. We have a Ventana > NexES Sp. Stainer and we order pre-made kits for our hand staining. Our > paths want us to validate the lot #'s for our specials also. > > Thanks..Joyce :-) > > > ***** CONFIDENTIALITY NOTICE ***** > This message contains confidential information and is intended only for > the individual named. If you are not the named addressee you should not > disseminate, distribute or copy this e-mail. Please notify the sender > immediately by e-mail if you have received this e-mail by mistake and > delete this e-mail from your system. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From KLAPANO1 <@t> hfhs.org Wed Apr 30 09:43:13 2008 From: KLAPANO1 <@t> hfhs.org (Karen Lapanowski) Date: Wed Apr 30 09:43:37 2008 Subject: [Histonet] White matter necrosis Message-ID: <48184D41020000280000424B@gwia2v.net.hfh.edu> Hello, Is there a way of detecting white matter necrosis myelinopathy versus myelin? Any suggestions concerning how to quantify myelinopathy would be appreciated. Thanks, Karen Lapanowski Karen Lapanowski Henry Ford Health System Radiation Oncology 3065 E & R 313-916-9386 ============================================================================== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================== From tony.j.savage <@t> gsk.com Wed Apr 30 09:51:40 2008 From: tony.j.savage <@t> gsk.com (tony.j.savage@gsk.com) Date: Wed Apr 30 09:52:20 2008 Subject: [Histonet] unsubscribe In-Reply-To: <200804231658.m3NGw4R7021082@rtpsfimr001.glaxo.com> Message-ID: Unsubscribe Regards, Tony Histology Section (Stevenage Core - DTG) Molecular Discovery Research. GlaxoSmithKline Medicines Research Centre, Gunnelswood Road, STEVENAGE, Hertfordshire. SG1 2NY tel. +44 (0)1438 764117 fax. +44 (0)1438 764782 email. Tony.J.Savage@gsk.com mobile +44 07753609835 http://ukdiscovery.gsk.com/histopathology/default.htm http://iwhf.gsk.com/targetdiscovery/targetlocalisation/default.htm ----------------------------------------------------------- This e-mail was sent by GlaxoSmithKline Services Unlimited (registered in England and Wales No. 1047315), which is a member of the GlaxoSmithKline group of companies. The registered address of GlaxoSmithKline Services Unlimited is 980 Great West Road, Brentford, Middlesex TW8 9GS. ----------------------------------------------------------- From dermpathsy <@t> gmail.com Wed Apr 30 10:06:26 2008 From: dermpathsy <@t> gmail.com (Sate Hamza) Date: Wed Apr 30 10:06:36 2008 Subject: [Histonet] Fwd: [Dermpath-l] query re microwave tissue processors In-Reply-To: References: Message-ID: <8854ff80804300806r76f91e4fl5bb1e64ff5d732c6@mail.gmail.com> Dear colleagues, This message was posted by a dermatopathologist on a dermatopathology mailing list. I wonder if anyone here would have any comments.. Thank you .. Best regards, Sate ---------- Forwarded message ---------- From: Date: Wed, Apr 30, 2008 at 8:46 AM Subject: [Dermpath-l] query re microwave tissue processors To: dermpath-l@lists.umanitoba.ca I am considering the purchase of a microwave tissue processor for skin punch, shave, and smaller skin biopsies with an onsite demo scheduled in a week or two. Do any of you have experience with the quality of these devices in terms of H&E, routine special stains, and immunohistochemical stains in the field of dermatopathology? I am looking at the Shandon TissueWave 2. -- Sate Hamza, MD, FRCPC Dermatopathologist Winnipeg, Canada From brett_connolly <@t> merck.com Wed Apr 30 10:32:05 2008 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Wed Apr 30 10:32:14 2008 Subject: [Histonet] SBEM antibody Message-ID: <63EA0607835FBA4689CEA9EA8B482692010C669B@usctmx1141.merck.com> Anyone know of a supplier for SBEM (small breast epithelial protein) for IHC in FFPE tissues?? Thanks much, Brett Brett M. Connolly, Ph.D. Research Fellow MRL, Imaging Research Merck & Co., Inc. WP-44K PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From lgruman <@t> mcw.edu Wed Apr 30 10:34:39 2008 From: lgruman <@t> mcw.edu (Gruman, Lynn) Date: Wed Apr 30 10:34:31 2008 Subject: [Histonet] Best place to purchase Tissue Arrays Message-ID: Can anyone suggest the best place to purchase prostate tissue arrays. Thanks, Lynn Gruman, HT(ASCP) Program Coordinator II Histology and Imaging Core Medical College of Wisconsin 8701 Watertown Plank Road CRI/TBRC Building 4th Floor/ C4470 Milwaukee, WI 53226 Phone: 414-955-8624 Fax: 414-955-6411 Email: lgruman@mcw.edu From hymclab <@t> hyhc.com Wed Apr 30 10:45:34 2008 From: hymclab <@t> hyhc.com (hymclab) Date: Wed Apr 30 10:44:32 2008 Subject: [Histonet] Her2 fixation tracking Message-ID: We just started putting the total time in formalin (including time on the tissue processor) in the last line of the gross description. We have radiology, OR techs, etc... Putting the time the specimen goes into formalin right on the requisition. We do not put a specimen on the processor if it comes after 3:00 pm as it will not meet the 6 hour threshold. Hope this helps, Dawn D. Schneider, HT(ASCP) Howard Young Medical Center Woodruff, WI 54568 (715)356-8174 schneiderd@hyhc.com -----Original Message----- From: fieldsfamily15@bellsouth.net [mailto:fieldsfamily15@bellsouth.net] Sent: Tuesday, April 29, 2008 10:24 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Her2 fixation tracking Hi Histo techs, Could you please let me know how you are handling tracking the time your specimens for Her 2 go into formalin? I need to know if you have the doctors put the time the tissue goes into formalin on the container or it is on a log or what... I am trying to see what solutions are working for you. I need to make sure the time in fixation plus the time on the processors is within the CAP recommended range. We are sending the Her2's out for the immunos but we have to document the total fixation time. Hope this makes sense...and thank you for any help. Carole Fields Northside Hospital Atlanta, GA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kolekams <@t> trinity-health.org Wed Apr 30 11:06:14 2008 From: kolekams <@t> trinity-health.org (Sarah Kolekamp) Date: Wed Apr 30 11:06:30 2008 Subject: [Histonet] Re: Negative Control Tissue Message-ID: <481860B60200006100012174@nodcgwcluster_gw4_server.no.trinity-health.org> I have a questions about negative control tissue, does anyone know what the most universal tissue would be that we could use to act as our negative control tissue. We are looking for the tissue that could be used with the widest array of antibodies. Thanks! Sarah Kolekamp HTL (ASCP) Lead Histotechnologist Saint Mary's Health Care Phone Number (616) 752-6155 Fax Number (616) 774-0628 From fong <@t> zoology.ubc.ca Wed Apr 30 11:11:09 2008 From: fong <@t> zoology.ubc.ca (Angelina Fong) Date: Wed Apr 30 11:11:43 2008 Subject: [Histonet] HRP travel times and speed In-Reply-To: <48177075.5020003@zoology.ubc.ca> References: <48177075.5020003@zoology.ubc.ca> Message-ID: <48189A1D.20504@zoology.ubc.ca> Sorry I wasn't very clear about what we are wanting to use HRP for. We are aiming to use HRP or WGA for neuronal tract tracing and wants to know how long we should leave the animal to recover for to allow sufficient travel time. Thanks > Hi histonetters, > > Does anyone know what the rate / speed of transport for HRP is? Does > anyone know a reference that has looked at or reports the rate of > transport of some common tracers like HRP or WGA? > > Thanks!! > > Cheers > Angelina > -- ~~o~~o~~o~~o~~o~~o~~o~~o~~o~~o Angelina Y. Fong, Ph.D. Department of Zoology Biological Sciences Building 6270 University Boulevard University of British Columbia Vancouver, BC, V6T 1Z4 Canada Ph: (604) 822-5990 Fax: (604) 822-2416 Email: fong@zoology.ubc.ca From POWELL_SA <@t> Mercer.edu Wed Apr 30 11:13:14 2008 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Wed Apr 30 11:18:09 2008 Subject: [Histonet] Help with antibodies Message-ID: <01MU8H54BF420034CT@Macon2.Mercer.edu> Hi Guys, I work in a research facility and we have a med student working on a project that requires using the antibody chemokine receptor, CXCR4, and also Epstein Barr virus, LMP1. I would appreciate any information from those of you who have successfully used either. I have been out of the IHC business for some time and need input. Thanks, Shirley Powell Mercer University School of Medicine Macon, GA 478-301-2374 From brett_connolly <@t> merck.com Wed Apr 30 11:20:12 2008 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Wed Apr 30 11:20:21 2008 Subject: [Histonet] RE: SBEM antibody Message-ID: <63EA0607835FBA4689CEA9EA8B482692010C66D5@usctmx1141.merck.com> make that small breast epithelial mucin (SBEM) > _____________________________________________ > From: Connolly, Brett M > Sent: Wednesday, April 30, 2008 11:32 AM > To: histonet@lists.utsouthwestern.edu; ihcrg@googlegroups.com > Subject: SBEM antibody > > Anyone know of a supplier for SBEM (small breast epithelial protein) > for IHC in FFPE tissues?? > > Thanks much, > Brett > > Brett M. Connolly, Ph.D. > Research Fellow > MRL, Imaging Research > Merck & Co., Inc. > WP-44K > PO Box 4 > West Point, PA 19486 > PH 215-652-2501 > fax. 215-993-6803 > e-mail. brett_connolly@merck.com > Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From mpence <@t> grhs.net Wed Apr 30 12:05:13 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Apr 30 12:05:19 2008 Subject: [Histonet] Re: Negative Control Tissue In-Reply-To: <481860B60200006100012174@nodcgwcluster_gw4_server.no.trinity-health.org> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3808@IS-E2K3.grhs.net> I think you would have to use the same tissue you use for your + control to show that it "is working/staining" and "is not working/staining" At least that is how we do it. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah Kolekamp Sent: Wednesday, April 30, 2008 11:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Negative Control Tissue I have a questions about negative control tissue, does anyone know what the most universal tissue would be that we could use to act as our negative control tissue. We are looking for the tissue that could be used with the widest array of antibodies. Thanks! Sarah Kolekamp HTL (ASCP) Lead Histotechnologist Saint Mary's Health Care Phone Number (616) 752-6155 Fax Number (616) 774-0628 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Wed Apr 30 12:12:31 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Apr 30 12:12:35 2008 Subject: [Histonet] Re: Negative Control Tissue In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3808@IS-E2K3.grhs.net> References: <481860B60200006100012174@nodcgwcluster_gw4_server.no.trinity-health.org> <661949901A768E4F9CC16D8AF8F2838C017A3808@IS-E2K3.grhs.net> Message-ID: <982A0A9461F9BF438C7B19A6E425A3830F9A9A@ITSSSXM01V6.one.ads.che.org> This has always been confusing, but, if I understand correctly, the negative control is the same patient tissue without primary antibody. Use a negative for each detection system used if you have multiple antibodies on the same block. Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, April 30, 2008 1:05 PM To: Sarah Kolekamp; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Negative Control Tissue I think you would have to use the same tissue you use for your + control to show that it "is working/staining" and "is not working/staining" At least that is how we do it. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah Kolekamp Sent: Wednesday, April 30, 2008 11:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Negative Control Tissue I have a questions about negative control tissue, does anyone know what the most universal tissue would be that we could use to act as our negative control tissue. We are looking for the tissue that could be used with the widest array of antibodies. Thanks! Sarah Kolekamp HTL (ASCP) Lead Histotechnologist Saint Mary's Health Care Phone Number (616) 752-6155 Fax Number (616) 774-0628 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From nrutledge <@t> CapeCodHealth.org Wed Apr 30 12:13:42 2008 From: nrutledge <@t> CapeCodHealth.org (Rutledge, Nancy) Date: Wed Apr 30 12:13:45 2008 Subject: [Histonet] Pneumocystis control slides Message-ID: <47AD3B259E920D449F580E6AE82C2B8F210244@FHEXSVR2.FHDOMAIN1.capecodhealth.org> Hoping someone knows of a supplier of pneumocystis TISSUE control slides? Thanks Nancy Rutledge ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org From Crystalmo <@t> fmchealth.org Wed Apr 30 12:24:03 2008 From: Crystalmo <@t> fmchealth.org (Crystal Morris) Date: Wed Apr 30 12:24:13 2008 Subject: [Histonet] Old Recycler Message-ID: Does anyone know how I should go about disposing of an old xylene recycler t= hat no longer works? Thanks Crystal "Confidentiality Notice: This e-mail message, including any=0A= attachments is for the sole use of the intended recipient(s)=0A= and may contain confidential and privileged information.=0A= Any unauthorized review; use; disclosure or distribution=0A= is prohibited. If you are not the intended recipient,=0A= please contact the sender by reply e-mail and destroy all copies of=0A= the original message." From laurie.colbert <@t> huntingtonhospital.com Wed Apr 30 13:22:59 2008 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Wed Apr 30 13:23:03 2008 Subject: [Histonet] Job Opportunity in Pasadena, CA Message-ID: <57BE698966D5C54EAE8612E8941D768302E381C1@EXCHANGE3.huntingtonhospital.com> Huntington Memorial Hospital in Pasadena, CA has a per diem Histotech job opening available immediately. We are looking for a tech who is flexible and available to work approximately 15 hours a week. The schedule would be 6-8 hours every Monday, and other day(s) as needed based on workload, vacations, and sicknesses. The start hours would vary from 3:30 am to 5:00 am. We do not work on the weekends. If interested, please feel free to contact me directly and/or fax me your resume. Laurie Colbert (626) 397-8620 (626) 397-2187 fax From ROrr <@t> enh.org Wed Apr 30 14:39:17 2008 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Wed Apr 30 14:39:22 2008 Subject: [Histonet] seeking Loyola researcher Message-ID: Can anyone put me in touch with the research lab at Loyola University Medical Center that worked with the Smoothelin Antibody. This was presented at USCAP abstract number 118 on page 71in the USCAP guide. Thank you Becky Orr CLA,HT(ASCP)QIHC Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 From gayle.callis <@t> bresnan.net Wed Apr 30 17:23:06 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Wed Apr 30 17:23:09 2008 Subject: [Histonet] Re: Negative Control Tissue References: <481860B60200006100012174@nodcgwcluster_gw4_server.no.trinity-health.org><661949901A768E4F9CC16D8AF8F2838C017A3808@IS-E2K3.grhs.net> <982A0A9461F9BF438C7B19A6E425A3830F9A9A@ITSSSXM01V6.one.ads.che.org> Message-ID: <000701c8ab10$c366f4b0$6501a8c0@DHXTS541> I was taught that a true negative control should not be elimination of the primary antibody (NULL control) but use the immunoglobulin matching the host of the primary antibody and at the same concentration as the primary antibody. Some people use nonimmune serum, but we prefer using IgG, IgG isotype, or IgM. I also found out the hard way, in a research project, that using non immune normal serum was unacceptable to reviewers, and the whole, incredibly tedious and difficult immunogold staining had to be repeated before a manuscript was accepted. A very hard lesson, and now there is a collection of IgG or isotype controls for all immunostaining projects available. This immunoglobulin negative control can be used on a patient tissue. The Ig negative control is there to evaluate nonspecific staining in that particular tissue. If background occurs, then a source test may be necessary, and with elimination of a primary antibody, that is the first step in a background source test. There is an excellent discussion of negative controls, tissue or reagent controls, in the freebie, Dako's Education Guide, Immunohistochenisal staining methods, Fourth Edition and available as pdf on Dako Website. There are several ways of setting up a negative or positive control. Their comment on page 114 of this manual, verbatim, "Finally, the diluent itself may be used as an alternative, which, however is neither efficient nor desirable" leads me to think the immunoglobulin control is more acceptable. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 -- Original Message ----- From: "Weems, Joyce" To: "Mike Pence" ; "Sarah Kolekamp" ; Sent: Wednesday, April 30, 2008 11:12 AM Subject: RE: [Histonet] Re: Negative Control Tissue This has always been confusing, but, if I understand correctly, the negative control is the same patient tissue without primary antibody. Use a negative for each detection system used if you have multiple antibodies on the same block. Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, April 30, 2008 1:05 PM To: Sarah Kolekamp; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Negative Control Tissue I think you would have to use the same tissue you use for your + control to show that it "is working/staining" and "is not working/staining" At least that is how we do it. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah Kolekamp Sent: Wednesday, April 30, 2008 11:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Negative Control Tissue I have a questions about negative control tissue, does anyone know what the most universal tissue would be that we could use to act as our negative control tissue. We are looking for the tissue that could be used with the widest array of antibodies. Thanks! Sarah Kolekamp HTL (ASCP) Lead Histotechnologist Saint Mary's Health Care Phone Number (616) 752-6155 Fax Number (616) 774-0628 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anh2006 <@t> med.cornell.edu Wed Apr 30 17:37:19 2008 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Wed Apr 30 17:37:32 2008 Subject: [Histonet] Re: Negative Control Tissue In-Reply-To: <000701c8ab10$c366f4b0$6501a8c0@DHXTS541> References: <481860B60200006100012174@nodcgwcluster_gw4_server.no.trinity-health.org> <661949901A768E4F9CC16D8AF8F2838C017A3808@IS-E2K3.grhs.net> <982A0A9461F9BF438C7B19A6E425A3830F9A9A@ITSSSXM01V6.one.ads.che.org> <000701c8ab10$c366f4b0$6501a8c0@DHXTS541> Message-ID: I agree with everything said here by Gayle and I too only accept stains which have been run with IgG controls, preferable isotype specific IgG controls, at the precise same concentration as the primary. In addition, the IgG controls should ideally be from the same company as the primary as different companies purify their Abs with different methods/buffers etc - this isn't always possible or feasible within the budget, but it's a nice thing to aim for. But I just want to add that another critical negative control besides IgG controls for any antibody - especially and importantly when doing antibody workups - is to run a tissue processed precisely the same way and which is absolutely negative for your antigen alongside your test sample. If you see positive staining in your test sample but the no-antigen tissue control and IgG controls are negative, well then you can be almost 100% sure your stain is real. Andrea Hooper >I was taught that a true negative control should not be elimination >of the primary antibody (NULL control) but use the immunoglobulin >matching the host of the primary antibody and at the same >concentration as the primary antibody. Some people use nonimmune >serum, but we prefer using IgG, IgG isotype, or IgM. I also found >out the hard way, in a research project, that using non immune >normal serum was unacceptable to reviewers, and the whole, >incredibly tedious and difficult immunogold staining had to be >repeated before a manuscript was accepted. A very hard lesson, and >now there is a collection of IgG or isotype controls for all >immunostaining projects available. > >This immunoglobulin negative control can be used on a patient >tissue. The Ig negative control is there to evaluate nonspecific >staining in that particular tissue. If background occurs, then a >source test may be necessary, and with elimination of a primary >antibody, that is the first step in a background source test. > >There is an excellent discussion of negative controls, tissue or >reagent controls, in the freebie, Dako's Education Guide, >Immunohistochenisal staining methods, Fourth Edition and available >as pdf on Dako Website. There are several ways of setting up a >negative or positive control. Their comment on page 114 of this >manual, verbatim, "Finally, the diluent itself may be used as an >alternative, which, however is neither efficient nor desirable" >leads me to think the immunoglobulin control is more acceptable. > >Gayle M. Callis >HTL/HT/MT(ASCP) >Bozeman MT 59715 -- From sprice2003 <@t> gmail.com Wed Apr 30 22:20:29 2008 From: sprice2003 <@t> gmail.com (Sally Price) Date: Wed Apr 30 22:20:34 2008 Subject: [Histonet] Best place to purchase Tissue Arrays Message-ID: Lynn: May I suggest that you consider 'Elite-Q' controls from QC Sciences ( www.QCSciences.com )? Cheers, Sally ------------------------------ Message: 2 Date: Wed, 30 Apr 2008 10:34:39 -0500 From: "Gruman, Lynn" Subject: [Histonet] Best place to purchase Tissue Arrays To: Can anyone suggest the best place to purchase prostate tissue arrays. Thanks, Lynn Gruman, HT(ASCP) Program Coordinator II Histology and Imaging Core Medical College of Wisconsin 8701 Watertown Plank Road CRI/TBRC Building 4th Floor/ C4470 Milwaukee, WI 53226 Phone: 414-955-8624 Fax: 414-955-6411 Email: lgruman@mcw.edu From jkiernan <@t> uwo.ca Wed Apr 30 23:58:25 2008 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Apr 30 23:58:29 2008 Subject: [Histonet] Old Recycler Message-ID: You will have to advertise it the way an estate agent sells a house that's not fit for human habitation. It's a handyman's delight. Your apparatus, duly repaired and modified, may appeal to anyone in need of equipment for distillation of beer, wine or other liquids that require concentration to increase their purity and efficacy. Crystal Morris Crystalmo@fmchealth.org wrote: Date: Wednesday, April 30, 2008 13:25 Subject: [Histonet] Old Recycler To: histonet@lists.utsouthwestern.edu > Does anyone know how I should go about disposing of an old > xylene recycler that no longer works? > Thanks > Crystal