[Histonet] protocol for clearing tissue!

[Vest, Luther]

Dear histonet:

One of the pathology residents in our department has asked me to post
this question.

 

I am in search of a protocol for clearing tissue. This technique is used
commonly in vascular research to visualize blood vessels. I want to use
it to clear mouse lung tissue that contains blue-stained
(B-galactosidase activity) tumor nodules. Any suggestions?

Thanks

 

Luther Vest (HT ASCP)

VA-MD Regional College of Veterinary Medicine

Duckpond Dr. Phase III

Blacksburg, Va 24061-0443