From rjbuesa <@t> yahoo.com Sat Sep 1 08:27:39 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Sep 1 08:27:55 2007 Subject: [Histonet] education In-Reply-To: <8C9BA271C30FAD6-EB8-1DA1@mblk-d27.sysops.aol.com> Message-ID: <52638.27751.qm@web61215.mail.yahoo.com> Check the NSH website. Ren? J. godsgalnow@aol.com wrote: Does anyone out there know of a college that offers an online BS program via distance education for histotechs that have an Associates degree?? If not, somebody needs to. Roxanne ________________________________________________________________________ Email and AIM finally together. You've gotta check out free AOL Mail! - http://mail.aol.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Luggage? GPS? Comic books? Check out fitting gifts for grads at Yahoo! Search. From ernestinemiddleton <@t> yahoo.ca Sun Sep 2 12:18:13 2007 From: ernestinemiddleton <@t> yahoo.ca (Ernestine Middleton) Date: Sun Sep 2 12:18:30 2007 Subject: [Histonet] Positions opening Message-ID: <241169.20732.qm@web51502.mail.re2.yahoo.com> Hello, Montefiore Medical Center, Bronx, NY is looking for evening Histology Supervisor; BS degress, at 5 years working in histology laboratory; NY State license. No grossing specimen or frozen section is required with this position. We are also in need of temp-histo-technician and fulltime histo-technologist. Associate degree for the Technician and BS degree for the Technologist. NY State license is now required with all laboratory position. Send all resumes to: Ernestine Middleton, Manager 111 E. 210 St. & Gunhill Road Bronx, New York, 10467 Tel. 718-920-4157 Fax. 718-547-1920 email Emiddlet@montefiore.org --------------------------------- Be smarter than spam. See how smart SpamGuard is at giving junk email the boot with the All-new Yahoo! Mail From kevinclayton1979 <@t> gmail.com Mon Sep 3 04:49:48 2007 From: kevinclayton1979 <@t> gmail.com (Kevin Clayton) Date: Mon Sep 3 04:50:14 2007 Subject: [Histonet] How to use the Leica VT1000 M section thickness control Message-ID: <94c6d26c0709030249s71b71d4xb2d9f52b6fb32534@mail.gmail.com> Hola Histonetters! Background: I have recently been shown how to use a Leica VT1000 M vibratome. This is quite an old vibratome and section thickness is controlled by turning a knob on the side of the machine. This simply raises or lowers the blade. Now, I was told (by someone who had only just been shown himself to use it) that to select the thickness desired you turn the knob (clockwise) to the number on the knob. If you set it to 40, it will produce a section of 40 microns. Once you get a section you turn it all the way around to 40 again to get another section of 40 microns. This doesn't seem correct to me and seems to be producing sections much thicker than 40 microns (100 microns...?). To me, it seems that if you take at section at position 40, and want another at 40 microns, you should turn the knob next time to 80. Correct or not? Unfortunately, I can't find the manual (in the lab or online) and I don't speak the language here well enough to trust anything I'm told - especially if someone who speaks the language fluently doesn't seem to have it correct. I could be wrong of course. Anyway, thanks for reading and thanks everybody who's ever responded for making this a great resource. Kevin From jkiernan <@t> uwo.ca Mon Sep 3 11:16:22 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Mon Sep 3 11:17:36 2007 Subject: [Histonet] damage to dura; also cresyl violet Message-ID: The simplistic answer is that your control drilling injures the dura and (subtly) the underlying cortex. If PFC means prefrontal cortex, then damaging it causes behavioral changes! Is "a standard dremmel" the kind of drill sold in hardware and craft stores and advertized on TV? If so, it's too heavy for making hole in a rat's skull. For rat neurosrgery you need an old-fashioned low speed dental drill with an angled handpiece that can be held between the thumb and the index and middle fingers, and a near-spherical burr about 1.5mm across. You also need an operating microscope with lighting that doesn't cast shadows, so that you can see where you are drilling. You see the dura after going through the inner table of the skull. Try not to scrape the dura, especially if it contains a blood vessel near the point of your intended stereotaxic needle penetration. Cresyl violet is not ordinarily used to detect "infiltration of immune cells". Is is a cationic dye of the oxazine series (or a mixture of similar compounds) used for fairly selective staining of cytoplasmic rRNA (Nissl substance of neurons) and the DNA of glial nuclei and nuclei of small neurons. (In nuclei of larger neurons, the DNA is rather thinly spread out and therefore only faintly stained for the most part.) In non-nervous tissue, cell nuclei, cartilage matrix, mast cell granules etc are stained, but cresyl violet is used principally for cytoarchitectonic studies of grey matter. For examining "immune cells" you need a stain that shows the different types of leukocyte. Giemsa is good. Lillie's azure-eosin is more trouble but can give excellent results. Both methods also show neurons well. John Kiernan Anatomy, UWO London, Canada --- ----- Original Message ----- From: Caroline Bass Date: Friday, August 31, 2007 0:54 Subject: [Histonet] damage to dura To: Histonet > Hey guys, > > i have a question I thought may be good for the group. I > am doing > stereotax surgeries on rats, basically making lesions in > the > prefrontal cortex. There are two potential sham controls, > one where > a vehicle is injected the other where the holes are drilled > but > nothing is injected. So here's the problem. In the > no injection > sham (i.e. only holes are drilled) the animals display an > alteration > of the behavior we're interested in. The tissue looks > good, and > there is no sign of infiltration of immune cells in the PFC by > cresyl > violet. However, the dura does have some damage where the > drill > pierced the skull. We use a standard dremmel. > > Are there any suggestions for what could be going on? Is > there an > immune response we should specifically look for, or is > there > something else about the dura that could be affecting our > system? > Does anyone know of how dura damage can alter nearby brain areas? > > Any and all advice is appreciated. > > Caroline > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Kemlo.Rogerson <@t> waht.swest.nhs.uk Tue Sep 4 05:25:53 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Sep 4 05:26:47 2007 Subject: [Histonet] damage to dura; also cresyl violet Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222EC4E@wahtntex2.waht.swest.nhs.uk> That sounds cruel; hide as there may be animal protectionists around, they'd blow you up in the UK!!!! Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Sunshine is delicious, rain is refreshing, wind braces us up, snow is exhilarating; there is really no such thing as bad weather, only different kinds of good weather. --John Ruskin This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From b-frederick <@t> northwestern.edu Tue Sep 4 08:34:11 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Tue Sep 4 08:34:33 2007 Subject: [Histonet] TUNEL In-Reply-To: <083120072317.3363.46D8A16E000DAA4000000D2322058860149D09020704040A0105@comcast.net> Message-ID: <006601c7eef8$4a55b720$d00f7ca5@lurie.northwestern.edu> You could probably run a Survivin antibody for comparasion. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of koellingr@comcast.net Sent: Friday, August 31, 2007 6:17 PM To: JR R; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] TUNEL Jerry, Could you expand on or give references to formalin causing DNA strand breaks? Double strand breaks, single strand, blunt end, overhanging? My pile of papers and having done TUNEL for years says that formalin fixation is a very good technique for TUNEL and many peer-reviewed articles in which TUNEL is used as a technique, use formalin fixation and how can that be if formalin is causing strand breaks? In fact one paper I'm looking at says that extended (5-7 weeks in formalin) fixation causes loss of TUNEL signal. If formalin is causing breaks, you would assume that TUNEL pos signals would increase with extended formalin fixation. Even the use of the monoclonal antibody F7-26, for single stranded DNA, touts formalin fixation for their claims of discriminating apoptosis from necrosis. The question asks about extended alcohol fixation but your answer is possibly a lot of false positives because formalin causes DNA breaks. Does this imply that alcohol won't? Have done a lot of TUNEL on alcohol fixed samples. True I couldn't pretreat them and handle them they way I would handle FFPE tissue. Also true that we could argue specificity and ability or not to discriminate apoptosis from necrosis for quite a while. But if formalin itself is causing the breaks in DNA, I and a lot of people are in big trouble with our science projects and experiments. Also I can't envision why 2 cells are showing TUNEL positivity while 2 of the same type of cells right next to them (and getting the same formalin fix), are absolutely clean and there is no background? Thanks for any information you can provide. Ray Koelling PhenoPath Laboratories Seattle, WA -------------- Original message -------------- From: JR R > > I expect you will get a very high background--lots of false positives. That's a > problem with TUNEL and formalin fixed tissue anyway--formalin causes DNA strand > breaks. > > > > Jerry L. Ricks > Research Scientist > U.W. Medicine at South Lake Union > 815 Mercer Street > Seattle, WA 98109 > (206)-685-7190> Date: Fri, 31 Aug 2007 10:42:32 +0200> From: > marijke.oste@ua.ac.be> To: histonet@lists.utsouthwestern.edu> Subject: > [Histonet] TUNEL> > For staining the apoptotic cells I'm already using a kit of > Roche> diagnostics. I think the greatest problem is that my tissues has been > saved> for several years in ethanol 70%. Does anyone has experience in staining> > tissues for apoptotic cells who are kept in ethanol for several years?> Thank > you> > > _______________________________________________> Histonet mailing list> > Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _________________________________________________________________ > Explore the seven wonders of the world > http://search.msn.com/results.aspx?q=7+wonders+world&mkt=en-US&form=QBRE____ ____ > _______________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hej01 <@t> health.state.ny.us Tue Sep 4 08:44:42 2007 From: hej01 <@t> health.state.ny.us (Helen E Johnson) Date: Tue Sep 4 08:44:55 2007 Subject: [Histonet] PT in a research lab Message-ID: Hi Histonetters, I'm inquiring if there is any Proficiency Testing for research laboratories. Helen Johnson (hej01@health.state.ny.us) IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. From dbpiontek <@t> hotmail.com Tue Sep 4 08:46:06 2007 From: dbpiontek <@t> hotmail.com (Denise Piontek) Date: Tue Sep 4 08:50:47 2007 Subject: [Histonet] Periodic Acid Formalin Fixation In-Reply-To: References: Message-ID: One last check for info after Labor Day:). Thanks for any advice per the question below: Dear Histonetters: I am performing Periodic Acid Formalin fixation on liver and noticing sinusoid shrinkage, as well as some edge effect? This is in comparison to a matched tissue control in 10% NBF. My recipe requires fixation at 4C for 48 hours via 1 gram of periodic acid in 100 mls 10% NBF. Anyone else using this fixation method? Any suggestions or advice is greatly appreciated, Denise Bland-Piontek, HTL(ASCP)CTBS(AATB)> NIBRI _________________________________________________________________ Connect to the next generation of MSN Messenger? http://imagine-msn.com/messenger/launch80/default.aspx?locale=en-us&source=wlmailtagline From rjbuesa <@t> yahoo.com Tue Sep 4 09:19:09 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Sep 4 09:19:27 2007 Subject: [Histonet] PT in a research lab In-Reply-To: Message-ID: <77038.95951.qm@web61213.mail.yahoo.com> Helen: Proficiency testings always refer to the tasks, not to the place where those tasks are completed. You will have to have the same proficiency to section, regardless of the setting, although perhaps minor variations could exist. Ren? J. Helen E Johnson wrote: Hi Histonetters, I'm inquiring if there is any Proficiency Testing for research laboratories. Helen Johnson (hej01@health.state.ny.us) IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Need a vacation? Get great deals to amazing places on Yahoo! Travel. From relia1 <@t> earthlink.net Tue Sep 4 09:37:31 2007 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Sep 4 09:41:13 2007 Subject: [Histonet] RELIA Histology Job Alert - NYC two positions. Message-ID: Hi Histonetters, I am currently working with a client in New York City looking for 2 histo techs. These are full time permanent positions. My client offers excellent compensation, benefits and a prestigious environment to work in. For more information please contact me at relia1@earthlink.net or toll free at 866-607-3542. Thanks and have a great day!! Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net From m5johnso <@t> meded.ucsd.edu Tue Sep 4 11:50:49 2007 From: m5johnso <@t> meded.ucsd.edu (Mindy Johnson) Date: Tue Sep 4 11:51:06 2007 Subject: [Histonet] In need of tissue and questions about restaining...... Message-ID: <00dc01c7ef13$bfa028b0$3ee07a10$@ucsd.edu> Hello to all.. I am fairly new to histonet. I am not a histologist by trade, but I am doing a bunch of histology work at UCSD. I have a few questions. Does anyone know where we can find some pathological tissue? We need this for students sets of pathology for our medical students. It has become pretty hard to find tissue. I scoured the internet and found Biomax, but that has been it. Also, since I have no formal training (I wish I would have taken histology courses in school) I need some advice on restaining some old tissue. This is for our student sets again. The stain has faded significantly. Most of the tissue is at least 15 yrs old. Some older. Some a about 10 yrs old. I have been soaking them in xylene for weeks and I can't seem to 1) either get the resin off the slide or 2) I have no clue what this option is as they have been soaking for so long and I do several changes!! I tried restaining some of they are pretty much ruined! So, I have some others and could really use some help! If there is any. Could the tissue be so old and used so often under the microscope that it has degraded and is not useful anymore? This is what I have been doing: Soak in xylene (do several changes) Bleach with potassium permanganate and oxalic acid. Restain with H&E protocol. But, that wasn't working and was not consistent at all. So I just tried putting the slides back in the hematoxylin after soaking in xylene and I got better results, with one type of tissue, but the older tissue didn't work so well. Thank you for all your help!!! Mindy A Johnson SRA II UCSD - School of Medicine Medical Teaching Labs Fax: 858-822-5250 From m5johnso <@t> meded.ucsd.edu Tue Sep 4 12:39:14 2007 From: m5johnso <@t> meded.ucsd.edu (Mindy Johnson) Date: Tue Sep 4 12:39:46 2007 Subject: [Histonet] In need of tissue and questions about restaining...... Message-ID: <00ea01c7ef1a$89cf2270$9d6d6750$@ucsd.edu> Thanks for the emails about the tissue.. But I can disregard that as I have found that our Medical Center has what I need! But, I could still use some trouble shooting help on my restaining issues... Thanks everyone! Mindy A Johnson SRA II UCSD - School of Medicine Medical Teaching Labs Fax: 858-822-5250 From: Mindy Johnson [mailto:m5johnso@meded.ucsd.edu] Sent: Tuesday, September 04, 2007 9:51 AM To: 'histonet@lists.utsouthwestern.edu' Subject: In need of tissue and questions about restaining...... Hello to all.. I am fairly new to histonet. I am not a histologist by trade, but I am doing a bunch of histology work at UCSD. I have a few questions. Does anyone know where we can find some pathological tissue? We need this for students sets of pathology for our medical students. It has become pretty hard to find tissue. I scoured the internet and found Biomax, but that has been it. Also, since I have no formal training (I wish I would have taken histology courses in school) I need some advice on restaining some old tissue. This is for our student sets again. The stain has faded significantly. Most of the tissue is at least 15 yrs old. Some older. Some a about 10 yrs old. I have been soaking them in xylene for weeks and I can't seem to 1) either get the resin off the slide or 2) I have no clue what this option is as they have been soaking for so long and I do several changes!! I tried restaining some of they are pretty much ruined! So, I have some others and could really use some help! If there is any. Could the tissue be so old and used so often under the microscope that it has degraded and is not useful anymore? This is what I have been doing: Soak in xylene (do several changes) Bleach with potassium permanganate and oxalic acid. Restain with H&E protocol. But, that wasn't working and was not consistent at all. So I just tried putting the slides back in the hematoxylin after soaking in xylene and I got better results, with one type of tissue, but the older tissue didn't work so well. Thank you for all your help!!! Mindy A Johnson SRA II UCSD - School of Medicine Medical Teaching Labs Fax: 858-822-5250 From Shirley_PHUA <@t> hsa.gov.sg Tue Sep 4 13:01:57 2007 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Tue Sep 4 13:08:49 2007 Subject: [Histonet] Shirley Phua is away on 04 Sep 2007 (Tuesday) afternoon. Message-ID: I will be out of the office from 04-09-2007 to 05-09-2007. I'll be away on 04 Sep 2007 (Tuesday) afternoon. I'll be back on 05 Sep 2007 (Wednesday). Pathologists: I will process your requests when I return. If urgent, please forward your email to Henry_Kyaw@hsa.gov.sg From eroux <@t> medimabs.com Tue Sep 4 13:23:19 2007 From: eroux <@t> medimabs.com (Emmanuelle Roux) Date: Tue Sep 4 13:24:26 2007 Subject: [Histonet] RE: In need of tissue and questions about In-Reply-To: <20070904165241.AFD9C6D805E@mx02.csee.onr.siteprotect.com> Message-ID: <20070904182410.ACE76150@mail.business.allstream.net> Hi Mindy, For finding tissues, try Asterand. They have a lot of human tissues, both normal and disease states. Emmanuelle Emmanuelle Roux, Ph.D. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: September 4, 2007 12:53 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 46, Issue 3 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: damage to dura; also cresyl violet (Kemlo Rogerson) 2. RE: TUNEL (Bernice Frederick) 3. PT in a research lab (Helen E Johnson) 4. RE: Periodic Acid Formalin Fixation (Denise Piontek) 5. Re: PT in a research lab (Rene J Buesa) 6. RELIA Histology Job Alert - NYC two positions. (Pam Barker) 7. In need of tissue and questions about restaining...... (Mindy Johnson) ---------------------------------------------------------------------- Message: 1 Date: Tue, 4 Sep 2007 11:25:53 +0100 From: "Kemlo Rogerson" Subject: RE: [Histonet] damage to dura; also cresyl violet To: "John Kiernan" , "Caroline Bass" Cc: Histonet Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222EC4E@wahtntex2.waht.swest.nhs.uk> Content-Type: text/plain; charset="us-ascii" That sounds cruel; hide as there may be animal protectionists around, they'd blow you up in the UK!!!! Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Sunshine is delicious, rain is refreshing, wind braces us up, snow is exhilarating; there is really no such thing as bad weather, only different kinds of good weather. --John Ruskin This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation ------------------------------ Message: 2 Date: Tue, 4 Sep 2007 08:34:11 -0500 From: "Bernice Frederick" Subject: RE: [Histonet] TUNEL To: , "'JR R'" , Message-ID: <006601c7eef8$4a55b720$d00f7ca5@lurie.northwestern.edu> Content-Type: text/plain; charset="US-ASCII" You could probably run a Survivin antibody for comparasion. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of koellingr@comcast.net Sent: Friday, August 31, 2007 6:17 PM To: JR R; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] TUNEL Jerry, Could you expand on or give references to formalin causing DNA strand breaks? Double strand breaks, single strand, blunt end, overhanging? My pile of papers and having done TUNEL for years says that formalin fixation is a very good technique for TUNEL and many peer-reviewed articles in which TUNEL is used as a technique, use formalin fixation and how can that be if formalin is causing strand breaks? In fact one paper I'm looking at says that extended (5-7 weeks in formalin) fixation causes loss of TUNEL signal. If formalin is causing breaks, you would assume that TUNEL pos signals would increase with extended formalin fixation. Even the use of the monoclonal antibody F7-26, for single stranded DNA, touts formalin fixation for their claims of discriminating apoptosis from necrosis. The question asks about extended alcohol fixation but your answer is possibly a lot of false positives because formalin causes DNA breaks. Does this imply that alcohol won't? Have done a lot of TUNEL on alcohol fixed samples. True I couldn't pretreat them and handle them they way I would handle FFPE tissue. Also true that we could argue specificity and ability or not to discriminate apoptosis from necrosis for quite a while. But if formalin itself is causing the breaks in DNA, I and a lot of people are in big trouble with our science projects and experiments. Also I can't envision why 2 cells are showing TUNEL positivity while 2 of the same type of cells right next to them (and getting the same formalin fix), are absolutely clean and there is no background? Thanks for any information you can provide. Ray Koelling PhenoPath Laboratories Seattle, WA -------------- Original message -------------- From: JR R > > I expect you will get a very high background--lots of false positives. That's a > problem with TUNEL and formalin fixed tissue anyway--formalin causes DNA strand > breaks. > > > > Jerry L. Ricks > Research Scientist > U.W. Medicine at South Lake Union > 815 Mercer Street > Seattle, WA 98109 > (206)-685-7190> Date: Fri, 31 Aug 2007 10:42:32 +0200> From: > marijke.oste@ua.ac.be> To: histonet@lists.utsouthwestern.edu> Subject: > [Histonet] TUNEL> > For staining the apoptotic cells I'm already using a kit of > Roche> diagnostics. I think the greatest problem is that my tissues has been > saved> for several years in ethanol 70%. Does anyone has experience in staining> > tissues for apoptotic cells who are kept in ethanol for several years?> Thank > you> > > _______________________________________________> Histonet mailing list> > Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _________________________________________________________________ > Explore the seven wonders of the world > http://search.msn.com/results.aspx?q=7+wonders+world&mkt=en-US&form=QBRE____ ____ > _______________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Tue, 4 Sep 2007 09:44:42 -0400 From: Helen E Johnson Subject: [Histonet] PT in a research lab To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII Hi Histonetters, I'm inquiring if there is any Proficiency Testing for research laboratories. Helen Johnson (hej01@health.state.ny.us) IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. ------------------------------ Message: 4 Date: Tue, 4 Sep 2007 09:46:06 -0400 From: Denise Piontek Subject: RE: [Histonet] Periodic Acid Formalin Fixation To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" One last check for info after Labor Day:). Thanks for any advice per the question below: Dear Histonetters: I am performing Periodic Acid Formalin fixation on liver and noticing sinusoid shrinkage, as well as some edge effect? This is in comparison to a matched tissue control in 10% NBF. My recipe requires fixation at 4C for 48 hours via 1 gram of periodic acid in 100 mls 10% NBF. Anyone else using this fixation method? Any suggestions or advice is greatly appreciated, Denise Bland-Piontek, HTL(ASCP)CTBS(AATB)> NIBRI _________________________________________________________________ Connect to the next generation of MSN Messenger http://imagine-msn.com/messenger/launch80/default.aspx?locale=en-us&source=w lmailtagline ------------------------------ Message: 5 Date: Tue, 4 Sep 2007 07:19:09 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] PT in a research lab To: Helen E Johnson , histonet@lists.utsouthwestern.edu Message-ID: <77038.95951.qm@web61213.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Helen: Proficiency testings always refer to the tasks, not to the place where those tasks are completed. You will have to have the same proficiency to section, regardless of the setting, although perhaps minor variations could exist. Reni J. Helen E Johnson wrote: Hi Histonetters, I'm inquiring if there is any Proficiency Testing for research laboratories. Helen Johnson (hej01@health.state.ny.us) IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Need a vacation? Get great deals to amazing places on Yahoo! Travel. ------------------------------ Message: 6 Date: Tue, 4 Sep 2007 10:37:31 -0400 From: "Pam Barker" Subject: [Histonet] RELIA Histology Job Alert - NYC two positions. To: "'Histonet'" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Histonetters, I am currently working with a client in New York City looking for 2 histo techs. These are full time permanent positions. My client offers excellent compensation, benefits and a prestigious environment to work in. For more information please contact me at relia1@earthlink.net or toll free at 866-607-3542. Thanks and have a great day!! Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net ------------------------------ Message: 7 Date: Tue, 4 Sep 2007 09:50:49 -0700 From: "Mindy Johnson" Subject: [Histonet] In need of tissue and questions about restaining...... To: Message-ID: <00dc01c7ef13$bfa028b0$3ee07a10$@ucsd.edu> Content-Type: text/plain; charset="US-ASCII" Hello to all.. I am fairly new to histonet. I am not a histologist by trade, but I am doing a bunch of histology work at UCSD. I have a few questions. Does anyone know where we can find some pathological tissue? We need this for students sets of pathology for our medical students. It has become pretty hard to find tissue. I scoured the internet and found Biomax, but that has been it. Also, since I have no formal training (I wish I would have taken histology courses in school) I need some advice on restaining some old tissue. This is for our student sets again. The stain has faded significantly. Most of the tissue is at least 15 yrs old. Some older. Some a about 10 yrs old. I have been soaking them in xylene for weeks and I can't seem to 1) either get the resin off the slide or 2) I have no clue what this option is as they have been soaking for so long and I do several changes!! I tried restaining some of they are pretty much ruined! So, I have some others and could really use some help! If there is any. Could the tissue be so old and used so often under the microscope that it has degraded and is not useful anymore? This is what I have been doing: Soak in xylene (do several changes) Bleach with potassium permanganate and oxalic acid. Restain with H&E protocol. But, that wasn't working and was not consistent at all. So I just tried putting the slides back in the hematoxylin after soaking in xylene and I got better results, with one type of tissue, but the older tissue didn't work so well. Thank you for all your help!!! Mindy A Johnson SRA II UCSD - School of Medicine Medical Teaching Labs Fax: 858-822-5250 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 46, Issue 3 *************************************** __________ NOD32 2502 (20070904) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From RSRICHMOND <@t> aol.com Tue Sep 4 13:40:03 2007 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Tue Sep 4 13:41:13 2007 Subject: [Histonet] Re: In need of tissue and questions about restaining Message-ID: Mindy A. Johnson at the University of California, San Diego (USCD) Medical Teaching Labs asks: >>Does anyone know where we can find some pathological tissue? We need this for students sets of pathology for our medical students. It has become pretty hard to find tissue. I scoured the internet and found Biomax, but that has been it.<< This is the responsibility of the pathologist in charge of the medical students' pathology course (often called the course master), and it can hardly be delegated to a histotechnologist (or in fact to anyone). Making a collection of pathologic specimens of sufficient quality for teaching beginners is a long labor indeed. Different medical schools have very different slide sets - some (well, 40 years ago) with as many as 300 different slides in them. I'd take this issue up with whatever pathologist you can talk to, but it's not your responsibility. Bob Richmond Samurai Pathologist Knoxville TN From rosenfeldtek <@t> hotmail.com Tue Sep 4 13:46:30 2007 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Tue Sep 4 13:46:45 2007 Subject: [Histonet] TUNEL, Formalin, DNA strand breaks In-Reply-To: <083120072317.3363.46D8A16E000DAA4000000D2322058860149D09020704040A0105@comcast.net> References: <083120072317.3363.46D8A16E000DAA4000000D2322058860149D09020704040A0105@comcast.net> Message-ID: Hi Ray, Sheesh-- I read (and then lost) the paper maybe 5-7 years ago, back when I was tearing my hair out over high background, or false positives for TUNEL stain in formalin fixed, paraffin embedded arterial sections. I can troubleshoot any immunostain, but the TUNEL and ISEL assays bedeviled me. The paper was titled something to the effect of "formaldehyde causes single and double stranded DNA breaks," and was pretty emphatic. I'll look for the article. I think the assay could work in whole cells for flow, or maybe in frozen tissue. My hunch at this point is that this is one of those rare cases where lots and lots of peer reviewed articles are just plain wrong. Hey, if someone has a good protocol, I'd be willing to try it out, and I would be delighted if I turn out to be mistaken. For now, I think TUNEL is the assay of the beast. Oh, here is a thought--say you are looking at a 5 micron section through a nucleus. The microtome blade pretty much had to create a lot of double stranded DNA breaks, no? Jerry L. Ricks Research Scientist U.W. Medicine at South Lake Union 815 Mercer Street Seattle, WA 98109 (206)-685-7190 From: koellingr@comcast.netTo: rosenfeldtek@hotmail.com; histonet@lists.utsouthwestern.eduSubject: RE: [Histonet] TUNELDate: Fri, 31 Aug 2007 23:17:03 +0000 Jerry, Could you expand on or give references to formalin causing DNA strand breaks? Double strand breaks, single strand, blunt end, overhanging? My pile of papers and having done TUNEL for years says that formalin fixation is a very good technique for TUNEL and many peer-reviewed articles in which TUNEL is used as a technique, use formalin fixation and how can that be if formalin is causing strand breaks? In fact one paper I'm looking at says that extended (5-7 weeks in formalin) fixation causes loss of TUNEL signal. If formalin is causing breaks, you would assume that TUNEL pos signals would increase with extended formalin fixation. Even the use of the monoclonal antibody F7-26, for single stranded DNA, touts formalin fixation for their claims of discriminating apoptosis from necrosis. The question asks about extended alcohol fixation but your answer is possibly a lot of false positives because formalin causes DNA breaks. Does this imply that alcohol won't? Have done a lot of TUNEL on alcohol fixed samples. True I couldn't pretreat them and handle them they way I would handle FFPE tissue. Also true that we could argue specificity and ability or not to discriminate apoptosis from necrosis for quite a while. But if formalin itself is causing the breaks in DNA, I and a lot of people are in big trouble with our science projects and experiments. Also I can't envision why 2 cells are showing TUNEL positivity while 2 of the same type of cells right next to them (and getting the same formalin fix), are absolutely clean and there is no background? Thanks for any information you can provide. Ray Koelling PhenoPath Laboratories Seattle, WA -------------- Original message -------------- From: JR R > > I expect you will get a very high background--lots of false positives. That's a > problem with TUNEL and formalin fixed tissue anyway--formalin causes DNA strand > breaks. > > > > Jerry L. Ricks > Research Scientist > U.W. Medicine at South Lake Union > 815 Mercer Street > Seattle, WA 98109 > (206)-685-7190> Date: Fri, 31 Aug 2007 10:42:32 +0200> From: > marijke.oste@ua.ac.be> To: histonet@lists.utsouthwestern.edu> Subject: > [Histonet] TUNEL> > For staining the apoptotic cells I'm already using a kit of > Roche> diagnostics. I think the greatest problem is that my tissues has been > saved> for several years in ethanol 70%. Does anyone has experience in staining> > tissues for apoptotic cells who are kept in ethanol for several years?> Thank > you> > > _______________________________________________> Histonet mailing list> > Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _________________________________________________________________ > Explore the seven wonders of the world > http://search.msn.com/results.aspx?q=7+wonders+world&mkt=en-US&form=QBRE________ > _______________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Discover the new Windows Vista http://search.msn.com/results.aspx?q=windows+vista&mkt=en-US&form=QBRE From ccross6032 <@t> aol.com Tue Sep 4 14:18:39 2007 From: ccross6032 <@t> aol.com (Cheryl Cross) Date: Tue Sep 4 14:19:07 2007 Subject: [Histonet] TUNEL, Formalin, DNA strand breaks In-Reply-To: References: <083120072317.3363.46D8A16E000DAA4000000D2322058860149D09020704040A0105@comcast.net> Message-ID: <4C1E074D-064A-45D1-BD32-13D7E2222B26@aol.com> Hi all - I have been round and round with this issue myself; I was basically told by people who've attempted it that TUNEL on FFPE sections is not specific enough due to the formalin issue. If you are trying to nail apoptosis, what about anti-active caspase-3? mind you, that staining can be a bit of a booger too, but it should be more sensitive and specific for apoptosis (no references to offer, i'm just repeating what i have been told). Cheryl Cross, DVM, Dipl. ACVP Researcher University Corporation for Atmospheric Research College of Veterinary Medicine University of Tennessee Department of Pathology 2407 River Drive, Room A201 Knoxville, TN 37996-4542 (423) 967-2724 fax: 865-974-5616 ccross@ucar.edu On Sep 4, 2007, at 2:46 PM, JR R wrote: > > Hi Ray, > > Sheesh-- I read (and then lost) the paper maybe 5-7 years ago, back > when I was tearing my hair out over high background, or false > positives for TUNEL stain in formalin fixed, paraffin embedded > arterial sections. I can troubleshoot any immunostain, but the > TUNEL and ISEL assays bedeviled me. > > The paper was titled something to the effect of "formaldehyde > causes single and double stranded DNA breaks," and was pretty > emphatic. I'll look for the article. > > > > I think the assay could work in whole cells for flow, or maybe in > frozen tissue. My hunch at this point is that this is one of those > rare cases where lots and lots of peer reviewed articles are just > plain wrong. > > Hey, if someone has a good protocol, I'd be willing to try it out, > and I would be delighted if I turn out to be mistaken. For now, I > think TUNEL is the assay of the beast. > > Oh, here is a thought--say you are looking at a 5 micron section > through a nucleus. The microtome blade pretty much had to create a > lot of double stranded DNA breaks, no? > > > Jerry L. Ricks > Research Scientist > U.W. Medicine at South Lake Union > 815 Mercer Street > Seattle, WA 98109 > (206)-685-7190 > > > From: koellingr@comcast.netTo: rosenfeldtek@hotmail.com; > histonet@lists.utsouthwestern.eduSubject: RE: [Histonet] TUNELDate: > Fri, 31 Aug 2007 23:17:03 +0000 > Jerry, > Could you expand on or give references to formalin causing DNA > strand breaks? Double strand breaks, single strand, blunt end, > overhanging? My pile of papers and having done TUNEL for years > says that formalin fixation is a very good technique for TUNEL and > many peer-reviewed articles in which TUNEL is used as a technique, > use formalin fixation and how can that be if formalin is causing > strand breaks? In fact one paper I'm looking at says that extended > (5-7 weeks in formalin) fixation causes loss of TUNEL signal. If > formalin is causing breaks, you would assume that TUNEL pos signals > would increase with extended formalin fixation. Even the use of > the monoclonal antibody F7-26, for single stranded DNA, touts > formalin fixation for their claims of discriminating apoptosis from > necrosis. > > The question asks about extended alcohol fixation but your answer > is possibly a lot of false positives because formalin causes DNA > breaks. Does this imply that alcohol won't? Have done a lot of > TUNEL on alcohol fixed samples. True I couldn't pretreat them and > handle them they way I would handle FFPE tissue. Also true that we > could argue specificity and ability or not to discriminate > apoptosis from necrosis for quite a while. But if formalin itself > is causing the breaks in DNA, I and a lot of people are in big > trouble with our science projects and experiments. Also I can't > envision why 2 cells are showing TUNEL positivity while 2 of the > same type of cells right next to them (and getting the same > formalin fix), are absolutely clean and there is no background? > > Thanks for any information you can provide. > > Ray Koelling > PhenoPath Laboratories > Seattle, WA > > -------------- Original message -------------- From: JR R > > > I expect you will get a very high > background--lots of false positives. That's a > problem with TUNEL > and formalin fixed tissue anyway--formalin causes DNA strand > > breaks. > > > > Jerry L. Ricks > Research Scientist > U.W. Medicine > at South Lake Union > 815 Mercer Street > Seattle, WA 98109 > > (206)-685-7190> Date: Fri, 31 Aug 2007 10:42:32 +0200> From: > > marijke.oste@ua.ac.be> To: histonet@lists.utsouthwestern.edu> > Subject: > [Histonet] TUNEL> > For staining the apoptotic cells I'm > already using a kit of > Roche> diagnostics. I think the greatest > problem is that my tissues has been > saved> for several years in > ethanol 70%. Does anyone has experience in staining> > tissues for > apoptotic cells who are kept in ethanol for several years?> Thank > > you> > > _______________________________________________> Histonet > mailing list> > Histonet@lists.utsouthwestern.edu> > http:// > lists.utsouthwestern.edu/mailman/listinfo/histonet > > _________________________________________________________________ > > Explore the seven wonders of the world > http://search.msn.com/ > results.aspx?q=7+wonders+world&mkt=en-US&form=QBRE________ > > _______________________________________ > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/ > mailman/listinfo/histonet > _________________________________________________________________ > Discover the new Windows Vista > http://search.msn.com/results.aspx?q=windows+vista&mkt=en- > US&form=QBRE_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MElliott <@t> mrl.ubc.ca Tue Sep 4 14:43:41 2007 From: MElliott <@t> mrl.ubc.ca (Mark Elliott) Date: Tue Sep 4 14:45:13 2007 Subject: [Histonet] Periostin and thrombospondin Message-ID: <46DD52FD020000D600024DE7@mail.mrl.ubc.ca> Anybody working with antibodies to these two proteins? Need to know what you are using for controls, and what retreival method, if any. Thanks very much. Mark ***CONFIDENTIALITY NOTICE*** This electronic message is intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From gvdobbin <@t> ihis.org Tue Sep 4 14:48:01 2007 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Tue Sep 4 14:48:27 2007 Subject: [Histonet] Need cassetes for Shurmark cassette labeller Message-ID: Hi Folks, We will soon be bringing a Shurmark Cassette Labeller online in our lab, and in preparation I have already started by purchasing appropriate cassettes (ie 45 degree angle face), the Histosette II from Fisher. These are not the more expensive ones that I know we "should" be using (ie the TBS cassettes), but they work fine with the labeller. The problem we are having is with a) the little box in side the cassette itself where the lid snaps shut, it interferes with the biopsy pads b) in taking the hinged lid off at embedding, the tissues sometimes fling out and c) they seem to be not as rigid as the Omnicette cassettes we used previously and so are compresssing in the chuck at cutting time. Can anyone suggest an alternative for a Shurmark labeller-compatible cassette that is not as expensive as the TBS ones? I have already switched to using the TBS slides for the slide inscriber with a significant shock to the "bean counters" of the organization. Just trying to avoid further shock if I can at all! Thanks in advance. Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ayant pour objet une personne ou un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer compl?tement de votre syst?me informatique. From AGrobe2555 <@t> aol.com Tue Sep 4 15:32:30 2007 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Tue Sep 4 15:32:53 2007 Subject: [Histonet] Alcain blue vs. Movats for GAG staining Message-ID: Hello All, Does anyone have a handle how GAG staining by Movats Pentachrome compares to low pH Alcian blue staining? I was asked to stain for GAGs in vascular sections (blood vessels and grafts), and was wondering which would be the best method (please suggest other methods). I suspect there won't be much staining for GAGs anyway. Please correct me if this is incorrect, as I have little experience in this area. Thanks, Albert ************************************** Get a sneak peek of the all-new AOL at http://discover.aol.com/memed/aolcom30tour From stamptrain <@t> yahoo.com Tue Sep 4 15:32:44 2007 From: stamptrain <@t> yahoo.com (Roger Moretz) Date: Tue Sep 4 15:32:57 2007 Subject: [Histonet] TUNEL, Formalin, DNA strand breaks In-Reply-To: <4C1E074D-064A-45D1-BD32-13D7E2222B26@aol.com> Message-ID: <283176.75757.qm@web55805.mail.re3.yahoo.com> Never had the problem with TUNEL on formalin fixed tissue sections. And I have also used the anti-cleaved caspase 3 technique. Both worked beautifully, but at this point, due to the fact the the IHC anti-cleaved caspase 3 method is simpler, that's the way I'd go. I am racking my retired brain to come up with names of vendors. So far no joy. Two months of retirement (as of today) seem to have put my memory in neutral. If I come up with them I'll get back to you. All the catalogs and methods are still with my former place of employment.... I have done the TUNEL stain to verify results, and the pathologist wanted to compare apples to apples, as it were. All the new work is being done with the caspase 3 IHC. Roger C. Moretz, Ph.D. (Ret.) --- Cheryl Cross wrote: > Hi all - > > I have been round and round with this issue myself; > I was basically > told by people who've attempted it that TUNEL on > FFPE sections is not > specific enough due to the formalin issue. > > If you are trying to nail apoptosis, what about > anti-active > caspase-3? mind you, that staining can be a bit of a > booger too, but > it should be more sensitive and specific for > apoptosis (no references > to offer, i'm just repeating what i have been told). > > > Cheryl Cross, DVM, Dipl. ACVP > Researcher > University Corporation for Atmospheric Research > College of Veterinary Medicine > University of Tennessee Department of Pathology > 2407 River Drive, Room A201 > Knoxville, TN 37996-4542 > (423) 967-2724 > fax: 865-974-5616 > ccross@ucar.edu > > > > > > On Sep 4, 2007, at 2:46 PM, JR R wrote: > > > > > Hi Ray, > > > > Sheesh-- I read (and then lost) the paper maybe > 5-7 years ago, back > > when I was tearing my hair out over high > background, or false > > positives for TUNEL stain in formalin fixed, > paraffin embedded > > arterial sections. I can troubleshoot any > immunostain, but the > > TUNEL and ISEL assays bedeviled me. > > > > The paper was titled something to the effect of > "formaldehyde > > causes single and double stranded DNA breaks," and > was pretty > > emphatic. I'll look for the article. > > > > > > > > I think the assay could work in whole cells for > flow, or maybe in > > frozen tissue. My hunch at this point is that > this is one of those > > rare cases where lots and lots of peer reviewed > articles are just > > plain wrong. > > > > Hey, if someone has a good protocol, I'd be > willing to try it out, > > and I would be delighted if I turn out to be > mistaken. For now, I > > think TUNEL is the assay of the beast. > > > > Oh, here is a thought--say you are looking at a 5 > micron section > > through a nucleus. The microtome blade pretty > much had to create a > > lot of double stranded DNA breaks, no? > > > > > > Jerry L. Ricks > > Research Scientist > > U.W. Medicine at South Lake Union > > 815 Mercer Street > > Seattle, WA 98109 > > (206)-685-7190 > > > > > > From: koellingr@comcast.netTo: > rosenfeldtek@hotmail.com; > > histonet@lists.utsouthwestern.eduSubject: RE: > [Histonet] TUNELDate: > > Fri, 31 Aug 2007 23:17:03 +0000 > > Jerry, > > Could you expand on or give references to formalin > causing DNA > > strand breaks? Double strand breaks, single > strand, blunt end, > > overhanging? My pile of papers and having done > TUNEL for years > > says that formalin fixation is a very good > technique for TUNEL and > > many peer-reviewed articles in which TUNEL is used > as a technique, > > use formalin fixation and how can that be if > formalin is causing > > strand breaks? In fact one paper I'm looking at > says that extended > > (5-7 weeks in formalin) fixation causes loss of > TUNEL signal. If > > formalin is causing breaks, you would assume that > TUNEL pos signals > > would increase with extended formalin fixation. > Even the use of > > the monoclonal antibody F7-26, for single stranded > DNA, touts > > formalin fixation for their claims of > discriminating apoptosis from > > necrosis. > > > > The question asks about extended alcohol fixation > but your answer > > is possibly a lot of false positives because > formalin causes DNA > > breaks. Does this imply that alcohol won't? Have > done a lot of > > TUNEL on alcohol fixed samples. True I couldn't > pretreat them and > > handle them they way I would handle FFPE tissue. > Also true that we > > could argue specificity and ability or not to > discriminate > > apoptosis from necrosis for quite a while. But if > formalin itself > > is causing the breaks in DNA, I and a lot of > people are in big > > trouble with our science projects and experiments. > Also I can't > > envision why 2 cells are showing TUNEL positivity > while 2 of the > > same type of cells right next to them (and getting > the same > > formalin fix), are absolutely clean and there is > no background? > > > > Thanks for any information you can provide. > > > > Ray Koelling > > PhenoPath Laboratories > > Seattle, WA > > > > -------------- Original message -------------- > From: JR R > > > > I expect you will > get a very high > > background--lots of false positives. That's a > > problem with TUNEL > > and formalin fixed tissue anyway--formalin causes > DNA strand > > > breaks. > > > > Jerry L. Ricks > Research > Scientist > U.W. Medicine > > at South Lake Union > 815 Mercer Street > Seattle, > WA 98109 > > > (206)-685-7190> Date: Fri, 31 Aug 2007 10:42:32 > +0200> From: > > > marijke.oste@ua.ac.be> To: > histonet@lists.utsouthwestern.edu> > > Subject: > [Histonet] TUNEL> > For staining the > apoptotic cells I'm > > already using a kit of > Roche> diagnostics. I > think the greatest > > problem is that my tissues has been > saved> for > several years in > > ethanol 70%. Does anyone has experience in > staining> > tissues for > > apoptotic cells who are kept in ethanol for > several years?> Thank > > > you> > > > _______________________________________________> > Histonet > > mailing list> > Histonet@lists.utsouthwestern.edu> > > http:// > > lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _________________________________________________________________ > > > > Explore the seven wonders of the world > > http://search.msn.com/ > > > results.aspx?q=7+wonders+world&mkt=en-US&form=QBRE________ > > > > _______________________________________ > Histonet > mailing list > > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/ > > mailman/listinfo/histonet > > > _________________________________________________________________ > > Discover the new Windows Vista > > > http://search.msn.com/results.aspx?q=windows+vista&mkt=en- > > > > US&form=QBRE_______________________________________________ > === message truncated === ____________________________________________________________________________________ Boardwalk for $500? In 2007? Ha! Play Monopoly Here and Now (it's updated for today's economy) at Yahoo! Games. http://get.games.yahoo.com/proddesc?gamekey=monopolyherenow From JWEEMS <@t> sjha.org Tue Sep 4 16:10:03 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Tue Sep 4 16:10:27 2007 Subject: [Histonet] RANTES Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FFA3E3@sjhaexc02.sjha.org> Checking for a source for this antibody. Anyone have a good one? Thanks, Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From failm <@t> musc.edu Tue Sep 4 16:11:42 2007 From: failm <@t> musc.edu (Mildred Fail) Date: Tue Sep 4 16:12:57 2007 Subject: [Histonet]questions about restaining...... Message-ID: Mindy, Try Heating the slides on a hot plate, the coverslips will usually pop right off after a few minutes. Extreme cold sometimes works too. As for restaining it just takes a little patience, generally they take a little longer to stain. Rena Fail >>> "Mindy Johnson" 09/04/07 01:39PM >>> Thanks for the emails about the tissue.. But I can disregard that as I have found that our Medical Center has what I need! But, I could still use some trouble shooting help on my restaining issues... Thanks everyone! Mindy A Johnson SRA II UCSD - School of Medicine Medical Teaching Labs Fax: 858-822-5250 From: Mindy Johnson [mailto:m5johnso@meded.ucsd.edu] Sent: Tuesday, September 04, 2007 9:51 AM To: 'histonet@lists.utsouthwestern.edu' Subject: In need of tissue and questions about restaining...... Hello to all.. I am fairly new to histonet. I am not a histologist by trade, but I am doing a bunch of histology work at UCSD. I have a few questions. Does anyone know where we can find some pathological tissue? We need this for students sets of pathology for our medical students. It has become pretty hard to find tissue. I scoured the internet and found Biomax, but that has been it. Also, since I have no formal training (I wish I would have taken histology courses in school) I need some advice on restaining some old tissue. This is for our student sets again. The stain has faded significantly. Most of the tissue is at least 15 yrs old. Some older. Some a about 10 yrs old. I have been soaking them in xylene for weeks and I can't seem to 1) either get the resin off the slide or 2) I have no clue what this option is as they have been soaking for so long and I do several changes!! I tried restaining some of they are pretty much ruined! So, I have some others and could really use some help! If there is any. Could the tissue be so old and used so often under the microscope that it has degraded and is not useful anymore? This is what I have been doing: Soak in xylene (do several changes) Bleach with potassium permanganate and oxalic acid. Restain with H&E protocol. But, that wasn't working and was not consistent at all. So I just tried putting the slides back in the hematoxylin after soaking in xylene and I got better results, with one type of tissue, but the older tissue didn't work so well. Thank you for all your help!!! Mindy A Johnson SRA II UCSD - School of Medicine Medical Teaching Labs Fax: 858-822-5250 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From failm <@t> musc.edu Tue Sep 4 16:14:52 2007 From: failm <@t> musc.edu (Mildred Fail) Date: Tue Sep 4 16:16:20 2007 Subject: [Histonet] Re: [Histone) More on questions about restaining...... Message-ID: Don't Bleach before restaining remove all the resin with xylene after removing the coverslip, rehydrate to water then repeat H&E only leave slides just a little longer even 20 yo slides will look like you stained them yesterday >>> "Mindy Johnson" 09/04/07 01:39PM >>> Thanks for the emails about the tissue.. But I can disregard that as I have found that our Medical Center has what I need! But, I could still use some trouble shooting help on my restaining issues... Thanks everyone! Mindy A Johnson SRA II UCSD - School of Medicine Medical Teaching Labs Fax: 858-822-5250 From: Mindy Johnson [mailto:m5johnso@meded.ucsd.edu] Sent: Tuesday, September 04, 2007 9:51 AM To: 'histonet@lists.utsouthwestern.edu' Subject: In need of tissue and questions about restaining...... Hello to all.. I am fairly new to histonet. I am not a histologist by trade, but I am doing a bunch of histology work at UCSD. I have a few questions. Does anyone know where we can find some pathological tissue? We need this for students sets of pathology for our medical students. It has become pretty hard to find tissue. I scoured the internet and found Biomax, but that has been it. Also, since I have no formal training (I wish I would have taken histology courses in school) I need some advice on restaining some old tissue. This is for our student sets again. The stain has faded significantly. Most of the tissue is at least 15 yrs old. Some older. Some a about 10 yrs old. I have been soaking them in xylene for weeks and I can't seem to 1) either get the resin off the slide or 2) I have no clue what this option is as they have been soaking for so long and I do several changes!! I tried restaining some of they are pretty much ruined! So, I have some others and could really use some help! If there is any. Could the tissue be so old and used so often under the microscope that it has degraded and is not useful anymore? This is what I have been doing: Soak in xylene (do several changes) Bleach with potassium permanganate and oxalic acid. Restain with H&E protocol. But, that wasn't working and was not consistent at all. So I just tried putting the slides back in the hematoxylin after soaking in xylene and I got better results, with one type of tissue, but the older tissue didn't work so well. Thank you for all your help!!! Mindy A Johnson SRA II UCSD - School of Medicine Medical Teaching Labs Fax: 858-822-5250 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Rena Fail From rosenfeldtek <@t> hotmail.com Tue Sep 4 16:23:50 2007 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Tue Sep 4 16:24:05 2007 Subject: [Histonet] TUNEL, Formalin, Caspase 3 In-Reply-To: <283176.75757.qm@web55805.mail.re3.yahoo.com> References: <4C1E074D-064A-45D1-BD32-13D7E2222B26@aol.com> <283176.75757.qm@web55805.mail.re3.yahoo.com> Message-ID: Hi Roger & Cheryl, Yeah I think I will try to go with the activated Caspase 3 stain. I just checked the Roche site and found that for TUNEL staining some tissues that give high false positives, one ought to use a Citrate buffer (pH 6.0) antigen retrieval step instead of a proteinase k digest. Apparently sacrificing a chicken under the full moon works sometimes, too. Jerry L. Ricks Research Scientist U.W. Medicine at South Lake Union 815 Mercer Street Seattle, WA 98109 (206)-685-7190 ----------------------------------------> Date: Tue, 4 Sep 2007 13:32:44 -0700> From: stamptrain@yahoo.com> To: ccross6032@aol.com; rosenfeldtek@hotmail.com> Subject: Re: [Histonet] TUNEL, Formalin, DNA strand breaks> CC: histonet@lists.utsouthwestern.edu>> Never had the problem with TUNEL on formalin fixed> tissue sections. And I have also used the> anti-cleaved caspase 3 technique. Both worked> beautifully, but at this point, due to the fact the> the IHC anti-cleaved caspase 3 method is simpler,> that's the way I'd go. I am racking my retired brain> to come up with names of vendors. So far no joy. Two> months of retirement (as of today) seem to have put my> memory in neutral. If I come up with them I'll get> back to you. All the catalogs and methods are still> with my former place of employment.... I have done> the TUNEL stain to verify results, and the pathologist> wanted to compare apples to apples, as it were. All> the new work is being done with the caspase 3 IHC.>> Roger C. Moretz, Ph.D. (Ret.)>> --- Cheryl Cross wrote:>>> Hi all ->>>> I have been round and round with this issue myself;>> I was basically>> told by people who've attempted it that TUNEL on>> FFPE sections is not>> specific enough due to the formalin issue.>>>> If you are trying to nail apoptosis, what about>> anti-active>> caspase-3? mind you, that staining can be a bit of a>> booger too, but>> it should be more sensitive and specific for>> apoptosis (no references>> to offer, i'm just repeating what i have been told).>>>>>> Cheryl Cross, DVM, Dipl. ACVP>> Researcher>> University Corporation for Atmospheric Research>> College of Veterinary Medicine>> University of Tennessee Department of Pathology>> 2407 River Drive, Room A201>> Knoxville, TN 37996-4542>> (423) 967-2724>> fax: 865-974-5616>> ccross@ucar.edu>>>>>>>>>>>> On Sep 4, 2007, at 2:46 PM, JR R wrote:>>>>>>>> Hi Ray,>>>>>> Sheesh-- I read (and then lost) the paper maybe>> 5-7 years ago, back>>> when I was tearing my hair out over high>> background, or false>>> positives for TUNEL stain in formalin fixed,>> paraffin embedded>>> arterial sections. I can troubleshoot any>> immunostain, but the>>> TUNEL and ISEL assays bedeviled me.>>>>>> The paper was titled something to the effect of>> "formaldehyde>>> causes single and double stranded DNA breaks," and>> was pretty>>> emphatic. I'll look for the article.>>>>>>>>>>>> I think the assay could work in whole cells for>> flow, or maybe in>>> frozen tissue. My hunch at this point is that>> this is one of those>>> rare cases where lots and lots of peer reviewed>> articles are just>>> plain wrong.>>>>>> Hey, if someone has a good protocol, I'd be>> willing to try it out,>>> and I would be delighted if I turn out to be>> mistaken. For now, I>>> think TUNEL is the assay of the beast.>>>>>> Oh, here is a thought--say you are looking at a 5>> micron section>>> through a nucleus. The microtome blade pretty>> much had to create a>>> lot of double stranded DNA breaks, no?>>>>>>>>> Jerry L. Ricks>>> Research Scientist>>> U.W. Medicine at South Lake Union>>> 815 Mercer Street>>> Seattle, WA 98109>>> (206)-685-7190>>>>>>>>> From: koellingr@comcast.netTo:>> rosenfeldtek@hotmail.com;>>> histonet@lists.utsouthwestern.eduSubject: RE:>> [Histonet] TUNELDate:>>> Fri, 31 Aug 2007 23:17:03 +0000>>> Jerry,>>> Could you expand on or give references to formalin>> causing DNA>>> strand breaks? Double strand breaks, single>> strand, blunt end,>>> overhanging? My pile of papers and having done>> TUNEL for years>>> says that formalin fixation is a very good>> technique for TUNEL and>>> many peer-reviewed articles in which TUNEL is used>> as a technique,>>> use formalin fixation and how can that be if>> formalin is causing>>> strand breaks? In fact one paper I'm looking at>> says that extended>>> (5-7 weeks in formalin) fixation causes loss of>> TUNEL signal. If>>> formalin is causing breaks, you would assume that>> TUNEL pos signals>>> would increase with extended formalin fixation.>> Even the use of>>> the monoclonal antibody F7-26, for single stranded>> DNA, touts>>> formalin fixation for their claims of>> discriminating apoptosis from>>> necrosis.>>>>>> The question asks about extended alcohol fixation>> but your answer>>> is possibly a lot of false positives because>> formalin causes DNA>>> breaks. Does this imply that alcohol won't? Have>> done a lot of>>> TUNEL on alcohol fixed samples. True I couldn't>> pretreat them and>>> handle them they way I would handle FFPE tissue.>> Also true that we>>> could argue specificity and ability or not to>> discriminate>>> apoptosis from necrosis for quite a while. But if>> formalin itself>>> is causing the breaks in DNA, I and a lot of>> people are in big>>> trouble with our science projects and experiments.>> Also I can't>>> envision why 2 cells are showing TUNEL positivity>> while 2 of the>>> same type of cells right next to them (and getting>> the same>>> formalin fix), are absolutely clean and there is>> no background?>>>>>> Thanks for any information you can provide.>>>>>> Ray Koelling>>> PhenoPath Laboratories>>> Seattle, WA>>>>>> -------------- Original message -------------->> From: JR R>>>>> I expect you will>> get a very high>>> background--lots of false positives. That's a>>> problem with TUNEL>>> and formalin fixed tissue anyway--formalin causes>> DNA strand>>>> breaks.>>>> Jerry L. Ricks> Research>> Scientist> U.W. Medicine>>> at South Lake Union> 815 Mercer Street> Seattle,>> WA 98109>>>> (206)-685-7190> Date: Fri, 31 Aug 2007 10:42:32>> +0200> From:>>>> marijke.oste@ua.ac.be> To:>> histonet@lists.utsouthwestern.edu>>>> Subject:> [Histonet] TUNEL>> For staining the>> apoptotic cells I'm>>> already using a kit of> Roche> diagnostics. I>> think the greatest>>> problem is that my tissues has been> saved> for>> several years in>>> ethanol 70%. Does anyone has experience in>> staining>> tissues for>>> apoptotic cells who are kept in ethanol for>> several years?> Thank>>>> you>>>>> _______________________________________________>>> Histonet>>> mailing list>> Histonet@lists.utsouthwestern.edu>>>> http://>>> lists.utsouthwestern.edu/mailman/listinfo/histonet>>>>>>>>> _________________________________________________________________>>>>>> Explore the seven wonders of the world>>> http://search.msn.com/>>>>>> results.aspx?q=7+wonders+world&mkt=en-US&form=QBRE________>>>>>> _______________________________________> Histonet>> mailing list>>>> Histonet@lists.utsouthwestern.edu>>> http://lists.utsouthwestern.edu/>>> mailman/listinfo/histonet>>>>>> _________________________________________________________________>>> Discover the new Windows Vista>>>>>> http://search.msn.com/results.aspx?q=windows+vista&mkt=en->>>>>>>> US&form=QBRE_______________________________________________>>> === message truncated ===>>>>> ____________________________________________________________________________________> Boardwalk for $500? In 2007? Ha! Play Monopoly Here and Now (it's updated for today's economy) at Yahoo! Games.> http://get.games.yahoo.com/proddesc?gamekey=monopolyherenow>> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Connect to the next generation of MSN Messenger? http://imagine-msn.com/messenger/launch80/default.aspx?locale=en-us&source=wlmailtagline From BoozerKA <@t> ah.org Tue Sep 4 18:23:38 2007 From: BoozerKA <@t> ah.org (Kathleen Boozer) Date: Tue Sep 4 18:24:45 2007 Subject: [Histonet] Microwaves Message-ID: <46DD8689.4AA8.00C0.0@ah.org> What is the best microwave for a small lab using it only for heating Bouin's and Silver Nitrate for special stains? I just can't believe I would have to spend $30,000+ or slow down and use a waterbath. From rosenfeldtek <@t> hotmail.com Tue Sep 4 18:58:53 2007 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Tue Sep 4 18:59:06 2007 Subject: [Histonet] Microwaves In-Reply-To: <46DD8689.4AA8.00C0.0@ah.org> References: <46DD8689.4AA8.00C0.0@ah.org> Message-ID: I use a plain old GE turntable microwave for that. It was like $150.00. You do want the turntable kind and with a digital timer is best. What you want to do is test the microwave to see exactly how long it takes to heat your solution to the required temperature in the exact container (staining tub, probably?) that you will be using. Jerry L. Ricks Research Scientist U.W. Medicine at South Lake Union 815 Mercer Street Seattle, WA 98109 (206)-685-7190> Date: Tue, 4 Sep 2007 16:23:38 -0700> From: BoozerKA@ah.org> To: histonet@lists.utsouthwestern.edu> Subject: [Histonet] Microwaves> > What is the best microwave for a small lab using it only for heating Bouin's and Silver Nitrate for special stains? I just can't believe I would have to spend $30,000+ or slow down and use a waterbath.> > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Connect to the next generation of MSN Messenger? http://imagine-msn.com/messenger/launch80/default.aspx?locale=en-us&source=wlmailtagline From tgoodpas <@t> fhcrc.org Tue Sep 4 20:24:26 2007 From: tgoodpas <@t> fhcrc.org (Goodpaster, Tracy A) Date: Tue Sep 4 20:24:42 2007 Subject: [Histonet] IHC anti-cleaved caspase 3 In-Reply-To: <283176.75757.qm@web55805.mail.re3.yahoo.com> References: <4C1E074D-064A-45D1-BD32-13D7E2222B26@aol.com> <283176.75757.qm@web55805.mail.re3.yahoo.com> Message-ID: <9214508BF36C4D4697A25230CE97EE41024BE4BD@groucho.fhcrc.org> We use Biocare's cleaved caspase-3 (cat# CP229B) at 0.21 ug/ml for 30 minutes. We use it on formalin fixed paraffin embedded sections after high pH steam antigen retrieval for 15 minutes with 15 minute cool down. Trilogy or pH 6.0 citrate buffer also work for antigen retrieval. Tonsil is a great control. Good Luck, Tracy Goodpaster -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roger Moretz Sent: Tuesday, September 04, 2007 1:33 PM To: Cheryl Cross; JR R Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] TUNEL, Formalin, DNA strand breaks Never had the problem with TUNEL on formalin fixed tissue sections. And I have also used the anti-cleaved caspase 3 technique. Both worked beautifully, but at this point, due to the fact the the IHC anti-cleaved caspase 3 method is simpler, that's the way I'd go. I am racking my retired brain to come up with names of vendors. So far no joy. Two months of retirement (as of today) seem to have put my memory in neutral. If I come up with them I'll get back to you. All the catalogs and methods are still with my former place of employment.... I have done the TUNEL stain to verify results, and the pathologist wanted to compare apples to apples, as it were. All the new work is being done with the caspase 3 IHC. Roger C. Moretz, Ph.D. (Ret.) --- Cheryl Cross wrote: > Hi all - > > I have been round and round with this issue myself; I was basically > told by people who've attempted it that TUNEL on FFPE sections is not > specific enough due to the formalin issue. > > If you are trying to nail apoptosis, what about anti-active caspase-3? > mind you, that staining can be a bit of a booger too, but it should be > more sensitive and specific for apoptosis (no references to offer, i'm > just repeating what i have been told). > > > Cheryl Cross, DVM, Dipl. ACVP > Researcher > University Corporation for Atmospheric Research College of Veterinary > Medicine University of Tennessee Department of Pathology > 2407 River Drive, Room A201 > Knoxville, TN 37996-4542 > (423) 967-2724 > fax: 865-974-5616 > ccross@ucar.edu > > > > > > On Sep 4, 2007, at 2:46 PM, JR R wrote: > > > > > Hi Ray, > > > > Sheesh-- I read (and then lost) the paper maybe > 5-7 years ago, back > > when I was tearing my hair out over high > background, or false > > positives for TUNEL stain in formalin fixed, > paraffin embedded > > arterial sections. I can troubleshoot any > immunostain, but the > > TUNEL and ISEL assays bedeviled me. > > > > The paper was titled something to the effect of > "formaldehyde > > causes single and double stranded DNA breaks," and > was pretty > > emphatic. I'll look for the article. > > > > > > > > I think the assay could work in whole cells for > flow, or maybe in > > frozen tissue. My hunch at this point is that > this is one of those > > rare cases where lots and lots of peer reviewed > articles are just > > plain wrong. > > > > Hey, if someone has a good protocol, I'd be > willing to try it out, > > and I would be delighted if I turn out to be > mistaken. For now, I > > think TUNEL is the assay of the beast. > > > > Oh, here is a thought--say you are looking at a 5 > micron section > > through a nucleus. The microtome blade pretty > much had to create a > > lot of double stranded DNA breaks, no? > > > > > > Jerry L. Ricks > > Research Scientist > > U.W. Medicine at South Lake Union > > 815 Mercer Street > > Seattle, WA 98109 > > (206)-685-7190 > > > > > > From: koellingr@comcast.netTo: > rosenfeldtek@hotmail.com; > > histonet@lists.utsouthwestern.eduSubject: RE: > [Histonet] TUNELDate: > > Fri, 31 Aug 2007 23:17:03 +0000 > > Jerry, > > Could you expand on or give references to formalin > causing DNA > > strand breaks? Double strand breaks, single > strand, blunt end, > > overhanging? My pile of papers and having done > TUNEL for years > > says that formalin fixation is a very good > technique for TUNEL and > > many peer-reviewed articles in which TUNEL is used > as a technique, > > use formalin fixation and how can that be if > formalin is causing > > strand breaks? In fact one paper I'm looking at > says that extended > > (5-7 weeks in formalin) fixation causes loss of > TUNEL signal. If > > formalin is causing breaks, you would assume that > TUNEL pos signals > > would increase with extended formalin fixation. > Even the use of > > the monoclonal antibody F7-26, for single stranded > DNA, touts > > formalin fixation for their claims of > discriminating apoptosis from > > necrosis. > > > > The question asks about extended alcohol fixation > but your answer > > is possibly a lot of false positives because > formalin causes DNA > > breaks. Does this imply that alcohol won't? Have > done a lot of > > TUNEL on alcohol fixed samples. True I couldn't > pretreat them and > > handle them they way I would handle FFPE tissue. > Also true that we > > could argue specificity and ability or not to > discriminate > > apoptosis from necrosis for quite a while. But if > formalin itself > > is causing the breaks in DNA, I and a lot of > people are in big > > trouble with our science projects and experiments. > Also I can't > > envision why 2 cells are showing TUNEL positivity > while 2 of the > > same type of cells right next to them (and getting > the same > > formalin fix), are absolutely clean and there is > no background? > > > > Thanks for any information you can provide. > > > > Ray Koelling > > PhenoPath Laboratories > > Seattle, WA > > > > -------------- Original message -------------- > From: JR R > > > > I expect you will > get a very high > > background--lots of false positives. That's a > > problem with TUNEL > > and formalin fixed tissue anyway--formalin causes > DNA strand > > > breaks. > > > > Jerry L. Ricks > Research > Scientist > U.W. Medicine > > at South Lake Union > 815 Mercer Street > Seattle, > WA 98109 > > > (206)-685-7190> Date: Fri, 31 Aug 2007 10:42:32 > +0200> From: > > > marijke.oste@ua.ac.be> To: > histonet@lists.utsouthwestern.edu> > > Subject: > [Histonet] TUNEL> > For staining the > apoptotic cells I'm > > already using a kit of > Roche> diagnostics. I > think the greatest > > problem is that my tissues has been > saved> for > several years in > > ethanol 70%. Does anyone has experience in > staining> > tissues for > > apoptotic cells who are kept in ethanol for > several years?> Thank > > > you> > > > _______________________________________________> > Histonet > > mailing list> > Histonet@lists.utsouthwestern.edu> > > http:// > > lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _________________________________________________________________ > > > > Explore the seven wonders of the world > > http://search.msn.com/ > > > results.aspx?q=7+wonders+world&mkt=en-US&form=QBRE________ > > > > _______________________________________ > Histonet > mailing list > > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/ > > mailman/listinfo/histonet > > > _________________________________________________________________ > > Discover the new Windows Vista > > > http://search.msn.com/results.aspx?q=windows+vista&mkt=en- > > > > US&form=QBRE_______________________________________________ > === message truncated === ________________________________________________________________________ ____________ Boardwalk for $500? In 2007? Ha! Play Monopoly Here and Now (it's updated for today's economy) at Yahoo! Games. http://get.games.yahoo.com/proddesc?gamekey=monopolyherenow _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BoozerKA <@t> ah.org Tue Sep 4 20:38:01 2007 From: BoozerKA <@t> ah.org (Kathleen Boozer) Date: Tue Sep 4 20:39:03 2007 Subject: [Histonet] Microwaves In-Reply-To: References: <46DD8689.4AA8.00C0.0@ah.org> Message-ID: <46DDA609.4AA8.00C0.0@ah.org> CAP now says "in accordance with manufacturer's instructions". In other words, food only, no chemicals. How do I get away with that? >>> JR R 09/04/2007 16:58 >>> I use a plain old GE turntable microwave for that. It was like $150.00. You do want the turntable kind and with a digital timer is best. What you want to do is test the microwave to see exactly how long it takes to heat your solution to the required temperature in the exact container (staining tub, probably?) that you will be using. Jerry L. Ricks Research Scientist U.W. Medicine at South Lake Union 815 Mercer Street Seattle, WA 98109 (206)-685-7190> Date: Tue, 4 Sep 2007 16:23:38 -0700> From: BoozerKA@ah.org> To: histonet@lists.utsouthwestern.edu> Subject: [Histonet] Microwaves> > What is the best microwave for a small lab using it only for heating Bouin's and Silver Nitrate for special stains? I just can't believe I would have to spend $30,000+ or slow down and use a waterbath.> > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Connect to the next generation of MSN Messenger http://imagine-msn.com/messenger/launch80/default.aspx?locale=en-us&source=wlmailtagline_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From turkekul <@t> gmail.com Tue Sep 4 23:34:04 2007 From: turkekul <@t> gmail.com (mesruh turkekul) Date: Tue Sep 4 23:34:18 2007 Subject: [Histonet] TUNEL, formalin DNA breaks, CLCas3 Message-ID: Dear All, When I do TUNEL on FFPE mouse or human tumors I always run TUNEL and ClCas3 on FFPE mouse embryos 12.5 days and mouse testis. Since I know exactly where the apoptotic cells are in the embryos and testis I see no difference between TUNEL and ClCas3. In some cases some tumors are positive with TUNEL but not positive with ClCas3 which means that TUNEL maybe also staining necrotic cells which can be identified by their morphology or it may indicate that there are some apoptotic pathways which does not involve activation of ClCas3 but eventfully form DNA breaks detected by TUNEL. If in one paper it is mentioned that formalin creates DNA breaks it does not mean it is true, on the contrary if there is only one paper and nobody reproduced this result after 6 years I will remain sceptical. I always trust papers and I always check their claims by controls. Regards, Mesruh Turkekul MSKCC MCCF www.mskcc.org New York, NY 10021 From koellingr <@t> comcast.net Wed Sep 5 00:03:38 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Wed Sep 5 00:03:51 2007 Subject: [Histonet] IHC anti-cleaved caspase 3 Message-ID: <090520070503.28973.46DE38AA00032D160000712D22007637049D09020704040A0105@comcast.net> This seems to have generated some variety of responses. I forwarded an e-mail to Dr. Lyon with an article showing 3-5 weeks in formalin (not 5-7 I had quickly mis-read) with reduced apoptotic signal in ISEL (I let the subtle difference between that and TUNEL slide). Is The Histochemical Journal vol. 27, number 12, December 1995 pp 983-988 F.D.Davison, et al. I actually did find a few articles dealing with DNA strand breaks and formaldehyde but all I believe are outside the realm and having little to do with this discussion. Again, my experience is that formalin fixation is good for TUNEL. Of course the devil is in the details and I hear about non-reactivity, background, false positives and I do not deny those things can occur but I believe there are ways to avoid them. Just doing TUNEL out of a kit is tantamount to getting an all-prediluted, IHC kit. It can work but then you start running into problems and you are stuck with the directions of the kit. Depending on my model, tissue, fixation I certainly amend the TUNEL protocol to fit my needs. Has little to do (I hope) with any superstitious allusion. You can certainly, and I have, altered the amount of Tdt in the mix. They use way to much in kit protocol but they want it to work, FOR SURE, and also sell a lot of expensive Tdt so really pour it on. Don't need that much. Tdt is template independent DNA polymerase so I vary time, I think they state way too high but again, they absolutely want staining there of some sort. Mix concentration I var y. Any who have done PCR knows the wild results you can get varying [Mg++]. Proteinase K, certainly there are other ways to get after the nucleoproteins (histones, etc) that will block the DNA enzyme from creating a longer strand. Someone mentioned citrate heat, its great. So I don't just use "a TUNEL kit". It can stain some stuff fine as is, but with a bit of ingenuity and thinking, always have been able to modify pretreatment, time, Tdt conc, Mg++ conc, to fit my model, tissue and type and time of fixation. My bet is that the drop off the authors saw in their study was due to the extended fixation of formalin affecting the nucleoproteins but them maybe not compensating by extending their pre-treatment. Just a guess. Someone mentioned survivin - great. Many mentioned cleaved caspace-3 and that is a fantastic antibody. With the giant caveat that it is well known that not all types of apoptosis go through the canonical caspace cascade. So depending on your model- you can miss apoptosis completely relying solely on caspace-3. Is great but not the total gold standard. I still believe TUNEL to be a fantastic tool which if used properly and with proper controls and variance of procedure, can lead to interpretable, no background results with with little worry of false positive and false negatives, IF you set things up properly. Then getting back to what I think was the original question. Someone had ?tissue in alcohol for extended periods. Have done this, just avoided a harsh pretreatment of long proteinase K which just chews up everything and with no formalin to have fixed it, your tissue ends up a mess instead of partially and correctly digested. I also am hoping I didn't spend my years suffering in grad school, eating cup-o-noodles every day with coffee after coffee to stay awake to study molecular immunology, molecular and cellular biology and signal transduction if sacrificing a chicken under a full moon (I hope I got the TUNEL protocol correct) was the simple answer on how to make this work. Raymond Koelling PhenoPath Laboratories Seattle, WA -------------- Original message -------------- From: "Goodpaster, Tracy A" > We use Biocare's cleaved caspase-3 (cat# CP229B) at 0.21 ug/ml for 30 > minutes. We use it on formalin fixed paraffin embedded sections after > high pH steam antigen retrieval for 15 minutes with 15 minute cool down. > Trilogy or pH 6.0 citrate buffer also work for antigen retrieval. > Tonsil is a great control. > Good Luck, > Tracy Goodpaster > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roger > Moretz > Sent: Tuesday, September 04, 2007 1:33 PM > To: Cheryl Cross; JR R > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] TUNEL, Formalin, DNA strand breaks > > Never had the problem with TUNEL on formalin fixed tissue sections. And > I have also used the anti-cleaved caspase 3 technique. Both worked > beautifully, but at this point, due to the fact the the IHC anti-cleaved > caspase 3 method is simpler, that's the way I'd go. I am racking my > retired brain to come up with names of vendors. So far no joy. Two > months of retirement (as of today) seem to have put my memory in > neutral. If I come up with them I'll get back to you. All the catalogs > and methods are still with my former place of employment.... I have > done the TUNEL stain to verify results, and the pathologist wanted to > compare apples to apples, as it were. All the new work is being done > with the caspase 3 IHC. > > Roger C. Moretz, Ph.D. (Ret.) > > --- Cheryl Cross wrote: > > > Hi all - > > > > I have been round and round with this issue myself; I was basically > > told by people who've attempted it that TUNEL on FFPE sections is not > > specific enough due to the formalin issue. > > > > If you are trying to nail apoptosis, what about anti-active caspase-3? > > > mind you, that staining can be a bit of a booger too, but it should be > > > more sensitive and specific for apoptosis (no references to offer, i'm > > > just repeating what i have been told). > > > > > > Cheryl Cross, DVM, Dipl. ACVP > > Researcher > > University Corporation for Atmospheric Research College of Veterinary > > Medicine University of Tennessee Department of Pathology > > 2407 River Drive, Room A201 > > Knoxville, TN 37996-4542 > > (423) 967-2724 > > fax: 865-974-5616 > > ccross@ucar.edu > > > > > > > > > > > > On Sep 4, 2007, at 2:46 PM, JR R wrote: > > > > > > > > Hi Ray, > > > > > > Sheesh-- I read (and then lost) the paper maybe > > 5-7 years ago, back > > > when I was tearing my hair out over high > > background, or false > > > positives for TUNEL stain in formalin fixed, > > paraffin embedded > > > arterial sections. I can troubleshoot any > > immunostain, but the > > > TUNEL and ISEL assays bedeviled me. > > > > > > The paper was titled something to the effect of > > "formaldehyde > > > causes single and double stranded DNA breaks," and > > was pretty > > > emphatic. I'll look for the article. > > > > > > > > > > > > I think the assay could work in whole cells for > > flow, or maybe in > > > frozen tissue. My hunch at this point is that > > this is one of those > > > rare cases where lots and lots of peer reviewed > > articles are just > > > plain wrong. > > > > > > Hey, if someone has a good protocol, I'd be > > willing to try it out, > > > and I would be delighted if I turn out to be > > mistaken. For now, I > > > think TUNEL is the assay of the beast. > > > > > > Oh, here is a thought--say you are looking at a 5 > > micron section > > > through a nucleus. The microtome blade pretty > > much had to create a > > > lot of double stranded DNA breaks, no? > > > > > > > > > Jerry L. Ricks > > > Research Scientist > > > U.W. Medicine at South Lake Union > > > 815 Mercer Street > > > Seattle, WA 98109 > > > (206)-685-7190 From jnocito <@t> satx.rr.com Wed Sep 5 06:17:27 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Sep 5 06:16:58 2007 Subject: [Histonet] Microwaves References: <46DD8689.4AA8.00C0.0@ah.org> <46DDA609.4AA8.00C0.0@ah.org> Message-ID: <006501c7efae$585175b0$d49eae18@yourxhtr8hvc4p> and people wonder why I can't stand CAP. Microwaves have been in use in the lab for over 20 years and all of a sudden you can't use house microwaves. Here's where I get flamed to the bone. I blame the microwave companies for this. I am in agreement that processing microwaves should be laboratory grade. I actually had an entire run burned to a crisp with an inferior laboratory grade microwave. Heating up a few solutions shouldn't require an $18,000 microwave. Now that I have stirred the pot, let the flaming begin. Oh yeah, this time, I'm not working, so there is no CEO to call (I really need to get a job. I have way too much time on my hands). OK, I have my flame retardant suit on. Let the flaming begin. JTT ----- Original Message ----- From: "Kathleen Boozer" To: "JR R" ; Sent: Tuesday, September 04, 2007 8:38 PM Subject: RE: [Histonet] Microwaves CAP now says "in accordance with manufacturer's instructions". In other words, food only, no chemicals. How do I get away with that? >>> JR R 09/04/2007 16:58 >>> I use a plain old GE turntable microwave for that. It was like $150.00. You do want the turntable kind and with a digital timer is best. What you want to do is test the microwave to see exactly how long it takes to heat your solution to the required temperature in the exact container (staining tub, probably?) that you will be using. Jerry L. Ricks Research Scientist U.W. Medicine at South Lake Union 815 Mercer Street Seattle, WA 98109 (206)-685-7190> Date: Tue, 4 Sep 2007 16:23:38 -0700> From: BoozerKA@ah.org> To: histonet@lists.utsouthwestern.edu> Subject: [Histonet] Microwaves> > What is the best microwave for a small lab using it only for heating Bouin's and Silver Nitrate for special stains? I just can't believe I would have to spend $30,000+ or slow down and use a waterbath.> > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Connect to the next generation of MSN Messenger http://imagine-msn.com/messenger/launch80/default.aspx?locale=en-us&source=wlmailtagline_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmerriam2003 <@t> yahoo.com Wed Sep 5 06:37:47 2007 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Wed Sep 5 06:37:59 2007 Subject: [Histonet] Alcain blue vs. Movats for GAG staining Message-ID: <791694.32512.qm@web50311.mail.re2.yahoo.com> In a past life, I used to use Alcian Blue for GAG staining (I can't remember if it was pH 1 or pH 2.5); we used to fix the tissues in Methacarn and that made a big difference in GAG retention (vs NBF). Good luck, Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA ----- Original Message ---- From: "AGrobe2555@aol.com" To: histonet@lists.utsouthwestern.edu Sent: Tuesday, September 4, 2007 4:32:30 PM Subject: [Histonet] Alcain blue vs. Movats for GAG staining Hello All, Does anyone have a handle how GAG staining by Movats Pentachrome compares to low pH Alcian blue staining? I was asked to stain for GAGs in vascular sections (blood vessels and grafts), and was wondering which would be the best method (please suggest other methods). I suspect there won't be much staining for GAGs anyway. Please correct me if this is incorrect, as I have little experience in this area. Thanks, Albert ************************************** Get a sneak peek of the all-new AOL at http://discover.aol.com/memed/aolcom30tour _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. http://farechase.yahoo.com/ From rjbuesa <@t> yahoo.com Wed Sep 5 07:41:43 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 5 07:41:57 2007 Subject: [Histonet] Microwaves In-Reply-To: <46DD8689.4AA8.00C0.0@ah.org> Message-ID: <930461.44417.qm@web61213.mail.yahoo.com> Kathleen: Microwave ovens (MWO) of all makes and models originally designed as home appliances heve been in use, specially for special stains, since the late 1970's By the mid 1990's tissue processing using MWO started to become more popular and household models were inadequate because of poor reproducibility, and no fumes controls. In consequence there was a task force on MWO that concluded that MWO should be used as "per manufacturers' specifications" and only MWO that could be vented were allowed in the laboratory. The outcry was so loud, and the disregard for the regulations were so frequent, that a sor of compromise was reached: 1- if you are going to process tissues, then you need a specially designed MWO with all the bells and whitles, starting at $8,000 or $9,000; 2- if you are going to use to heat solutions (like HIER, and other not giving off noxious fumes), you can use any household MWO, provided that you document reproducibility in your heating process; and 3- if you are going to use it for heating solutions that could produce noxious fumes, it has to be either vented, or placed insude a fumes hood. You also have to document reproducibility and have to calibrate it. The initial "draconian" prohibition was pushed by the laboratory MWO manufacturers, that wanted to open the market to their products, but a good household MWO well calibrated is perfectly suitable to heat reagents and solutions, and do not merit a capital investment. Under separate cover I am sending a paper of mine on how to calibrate your MWO. Ah, by the way, I always used household MWO for heating solutions and was never cited by CAP in any inspection, as long as I was able to show calibration curves and reproducibility information that, if you are going to boil distilled water, are useless, because water will boil at the same temperature (unless you live in Denver, where it will boil at LESS than 100?C, but that is another topic dealing with Her2/neu procedures). Hope this will help you! Ren? J. Kathleen Boozer wrote: What is the best microwave for a small lab using it only for heating Bouin's and Silver Nitrate for special stains? I just can't believe I would have to spend $30,000+ or slow down and use a waterbath. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! oneSearch: Finally, mobile search that gives answers, not web links. From koellingr <@t> comcast.net Wed Sep 5 07:57:22 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Wed Sep 5 07:57:36 2007 Subject: [Histonet] TUNEL, formalin DNA breaks, CLCas3 Message-ID: <090520071257.23915.46DEA7B2000A66E700005D6B22070029539D09020704040A0105@comcast.net> I agree, One of my lab mates in grad school used mouse developing embryos to look at apoptosis in the webbing between the forepaw digits during development. Those cells don't necrose. They go through a very specific programmed cell death. Then he was looking at the macrophages, by IHC, eating the apoptotic debris and whether those macs were resident or homing from other areas of the developing limb bud. This was before cleaved caspace-3 was available. His TUNEL was exquisite on formalin fixed mouse embryo's and reproducible for years. My heart and desire laid in the thymus and how T-lymphocytes undergo development as they survive to express a their T-cell repertoire and develop fully (less than 5%) or apoptose (95%) for a variety of reasons. His TUNEL procedure didn't work so well on my thymus and my tweaked procedure worked, but not so well on his mouse embryo's. In a former company, had a post-doc looking at myocardiocytes and a certain type of development and apoptosis they go th rough. She had to tweak the procedure a third way for her needs to get really fabulous staining. As Mesruh said, controls are vital and there are ways that cells can apoptose, not necrose, by alternate pathways than cleaved caspace-3, although it is certainly a very simple and reliable and wonderful IHC stain to do. Raymond Koelling PhenoPath Laboratories Seattle, WA -------------- Original message -------------- From: "mesruh turkekul" > Dear All, > > When I do TUNEL on FFPE mouse or human tumors I always run TUNEL and > ClCas3 on FFPE mouse embryos 12.5 days and mouse testis. Since I know > exactly where the apoptotic cells are in the embryos and testis I see > no difference between TUNEL and ClCas3. In some cases some tumors are > positive with TUNEL but not positive with ClCas3 which means that > TUNEL maybe also staining necrotic cells which can be identified by > their morphology or it may indicate that there are some apoptotic > pathways which does not involve activation of ClCas3 but eventfully > form DNA breaks detected by TUNEL. > If in one paper it is mentioned that formalin creates DNA breaks it > does not mean it is true, on the contrary if there is only one paper > and nobody reproduced this result after 6 years I will remain > sceptical. I always trust papers and I always check their claims by > controls. > > Regards, > Mesruh Turkekul > MSKCC MCCF > www.mskcc.org > New York, NY 10021 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bakevictoria <@t> gmail.com Wed Sep 5 08:02:16 2007 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Wed Sep 5 08:02:30 2007 Subject: [Histonet] Users of Lab Vision autostainer Message-ID: <4f016b690709050602w54725be5ob99e25453ed48bef@mail.gmail.com> Hi I'm a new user of the Lab Vision autostainer and I'm looking to see if I can find users in Histo-land that have experience with it. The facility only works with human tissue and all of the antibodies are for dx purposes. My key questions are as follows: How many antibodies is your lab running? How many users do you allow? How many people do you allow programming rights and at what level of supervision are they? How many of these antibodies are from Lab Vision? a) are they concentrates or pre-dilutes? b) for HIER are you using their PT modules/procedures or your own equipment (microwave, steamer, pressure cooker etc) and in house designed protocols for retrieval? c) for digestion do you only use their Pro-K or have you designed your own in-house methods using other reagents for digestion? d) do you put your controls on the same slide as the patient? e) are your controls in-house or commercial? f) do you have more than one stainer hooked up to one computer system? What Version of software do you currently have on your system? Any feed back would be very helpful. Thanks in advance. Vikki Baker Interim Histology Manager Mission Hospital System Asheville, NC From PMcArdle <@t> ebsciences.com Wed Sep 5 09:07:31 2007 From: PMcArdle <@t> ebsciences.com (Phil McArdle) Date: Wed Sep 5 09:07:55 2007 Subject: [Histonet] Microwaves VENDOR RESPONSE In-Reply-To: <46DD8689.4AA8.00C0.0@ah.org> References: <46DD8689.4AA8.00C0.0@ah.org> Message-ID: <46DEB823.4060708@ebsciences.com> Again, a microwave vendor weighs in (so far I haven't received any flames), so read at your own risk. :-) At the risk of sounding overly and overtly commercial, after reading post after post of $30,000+ and $18,000 and similarly high figures for lab microwaves, I really feel the need to set the record straight. Depending on the usage requirements, we have laboratory microwaves as low as $1749 for a "bare bones" model for simple operations, to mid-priced units, to under $11,000 for a vacuum equipped microwave processor capable of the +/- 0.5 degree C temperature control necessary for tissue processing. (I can feel the heat already!) There are many compelling reasons to replace a kitchen microwave with a lab model; feel free to download, read, and even share with colleagues our Microwave Companion at http://www.ebsciences.com/pdf/EBS_MW_COMPANION.pdf Best regards, and see you at NSH, Phil McArdle -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Mobile: 860.597.6796 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com Kathleen Boozer wrote: > What is the best microwave for a small lab using it only for heating Bouin's and Silver Nitrate for special stains? I just can't believe I would have to spend $30,000+ or slow down and use a waterbath. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet I skate to where the puck is going to be, not to where it's been. - Wayne Gretsky You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it, and it was probably my mistake anyway. So please do the right thing and make this e-mail go away. Thank you. From mcauliff <@t> umdnj.edu Wed Sep 5 09:16:27 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Sep 5 09:17:07 2007 Subject: [Histonet] Alcain blue vs. Movats for GAG staining In-Reply-To: References: Message-ID: <46DEBA3B.1000101@umdnj.edu> Hi Albert: As Kim mentioned fixing with an alcoholic fixative (methacarn or formalin-alcohol-acetic acid) will improve retention of GAG which should be plentiful in arteries. You can also put Safranin O (J. Ultrastructure Res. 54:451-460, 1976) or tannic acid (J. Histochem. Cytochem. 31:783-790, 1983) in the fixative to increase retention. Geoff AGrobe2555@aol.com wrote: > Hello All, > Does anyone have a handle how GAG staining by Movats Pentachrome compares to > low pH Alcian blue staining? I was asked to stain for GAGs in vascular > sections (blood vessels and grafts), and was wondering which would be the best > method (please suggest other methods). I suspect there won't be much staining > for GAGs anyway. Please correct me if this is incorrect, as I have little > experience in this area. > Thanks, > Albert > > > > ************************************** Get a sneak peek of the all-new AOL at > http://discover.aol.com/memed/aolcom30tour > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From rfisher <@t> gbmc.org Wed Sep 5 09:45:30 2007 From: rfisher <@t> gbmc.org (RENEE FISHER) Date: Wed Sep 5 09:46:48 2007 Subject: [Histonet] RDO decal Message-ID: <46DE88C9.09C2.00CA.0@gbmc.org> Has anyone heard of neutralizing RDO with baking soda to P.H. 7.0. Our Histo lab had a hazardous waste assessment, and the consultant suggested we not throw the RDO down the drain but that we either collect it for waste removal or neutralize it with baking soda to p.h. 7.0. I have not heard of doing this and do not know of any procedure for it, everyone, anyone's help will be greatly appreciated. Thanks, Renee' _______________________________________________________________________________________ This email may contain confidential protected health information and/or proprietary information belonging to the sender that is legally privileged under local, state, or federal law. This information is intended only for the use of the individual or individuals who have received this. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for the disposal of this information. From rjbuesa <@t> yahoo.com Wed Sep 5 10:06:35 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 5 10:06:48 2007 Subject: [Histonet] RDO decal In-Reply-To: <46DE88C9.09C2.00CA.0@gbmc.org> Message-ID: <484684.22847.qm@web61224.mail.yahoo.com> Ren?e: I have not heard of neutralizing RDO, but it would make sense if you want to be "gentle on your sewer system" BUT prepare to a large emission of carbon dioxide when attempting to neutralize it with baking soda. RDO + baking soda (or sodium bicarbonate) will produce water, salt with the acid in RDO (probably sodium chloride or common salt), and carbon dioxide in stoichiometrical amounts (1 CO2 per every 1 NaCl). Therefore that neutralization has to take place in a fumes hood, and you will have to decide which is worst: delivering acid to the sewer system, or carbon dioxide (the Greenhouse gas per excellence) to the atmosphere. It will be "your call". Ren? J. RENEE FISHER wrote: Has anyone heard of neutralizing RDO with baking soda to P.H. 7.0. Our Histo lab had a hazardous waste assessment, and the consultant suggested we not throw the RDO down the drain but that we either collect it for waste removal or neutralize it with baking soda to p.h. 7.0. I have not heard of doing this and do not know of any procedure for it, everyone, anyone's help will be greatly appreciated. Thanks, Renee' _______________________________________________________________________________________ This email may contain confidential protected health information and/or proprietary information belonging to the sender that is legally privileged under local, state, or federal law. This information is intended only for the use of the individual or individuals who have received this. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for the disposal of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: mail, news, photos & more. From jnocito <@t> satx.rr.com Wed Sep 5 10:56:28 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Sep 5 10:55:59 2007 Subject: [Histonet] Microwaves VENDOR RESPONSE References: <46DD8689.4AA8.00C0.0@ah.org> <46DEB823.4060708@ebsciences.com> Message-ID: <003b01c7efd5$52d0da00$d49eae18@yourxhtr8hvc4p> ok, but with the budgets today, many people can't afford a $1749 microwave when they can buy one at Walmart, K-Mart, or somewhere else for $79. Not to make you angry or anything, but I'm wondering how long has it been since you worked in a lab? Histo's budget is the first one cut in the lab because we are not essential. I can't count how many times I fought and fought for my budgets. If I tried to justify a $1749 microwave for special stains, HIER or whatever, I would have been laughed out the manager's office. Just my 4 cents. JTT ----- Original Message ----- From: "Phil McArdle" To: "Kathleen Boozer" Cc: Sent: Wednesday, September 05, 2007 9:07 AM Subject: Re: [Histonet] Microwaves VENDOR RESPONSE > Again, a microwave vendor weighs in (so far I haven't received any > flames), so read at your own risk. :-) > > At the risk of sounding overly and overtly commercial, after reading post > after post of $30,000+ and $18,000 and similarly high figures for lab > microwaves, I really feel the need to set the record straight. Depending > on the usage requirements, we have laboratory microwaves as low as $1749 > for a "bare bones" model for simple operations, to mid-priced units, to > under $11,000 for a vacuum equipped microwave processor capable of the +/- > 0.5 degree C temperature control necessary for tissue processing. > > (I can feel the heat already!) > > There are many compelling reasons to replace a kitchen microwave with a > lab model; feel free to download, read, and even share with colleagues our > Microwave Companion at > > http://www.ebsciences.com/pdf/EBS_MW_COMPANION.pdf > > Best regards, and see you at NSH, > > Phil McArdle > > -- > Phil McArdle > Microwave Product Manager > > Energy Beam Sciences, Inc. > 29-B Kripes Rd. > East Granby, CT 06026 > > Tel: 800.992.9037 x 341 > Mobile: 860.597.6796 > Fax: 860.653.0422 > > pmcardle@ebsciences.com > www.ebsciences.com > > Kathleen Boozer wrote: >> What is the best microwave for a small lab using it only for heating >> Bouin's and Silver Nitrate for special stains? I just can't believe I >> would have to spend $30,000+ or slow down and use a waterbath. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > I skate to where the puck is going to be, not to where it's been. > - Wayne Gretsky > > You must be the change you want to see in the world. > - Mahatma Gandhi > > NOTE: This message, together with any attachments, is intended only for > the use of the individual or entity to which it is addressed and may > contain information that is legally privileged, confidential and exempt > from disclosure. If you are not the intended recipient, however, there's > not a lot I can do about it, and it was probably my mistake anyway. So > please do the right thing and make this e-mail go away. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From johnzhan <@t> yahoo.com Wed Sep 5 11:13:13 2007 From: johnzhan <@t> yahoo.com (J. Zhan) Date: Wed Sep 5 11:13:25 2007 Subject: [Histonet] Your advice needed! Message-ID: <505635.97444.qm@web31713.mail.mud.yahoo.com> Hi, everyone: I am very new here, but quite experienced in Histology with a Doctor degree. Currently I am looking for a chance to get a license of Histologist from ASCP. To my knowlege, I need one-year recent lab experience to be qualified for such exam. Is there anybody who knows where I can find any internship or similar working opportunity? Your effort is appreciated in advance! John ____________________________________________________________________________________ Pinpoint customers who are looking for what you sell. http://searchmarketing.yahoo.com/ From PMcArdle <@t> ebsciences.com Wed Sep 5 11:16:52 2007 From: PMcArdle <@t> ebsciences.com (Phil McArdle) Date: Wed Sep 5 11:17:09 2007 Subject: [Histonet] Microwaves VENDOR RESPONSE In-Reply-To: <003b01c7efd5$52d0da00$d49eae18@yourxhtr8hvc4p> References: <46DD8689.4AA8.00C0.0@ah.org> <46DEB823.4060708@ebsciences.com> <003b01c7efd5$52d0da00$d49eae18@yourxhtr8hvc4p> Message-ID: <46DED674.8060704@ebsciences.com> Hi Joe: No argument there. I'm painfully aware of both a mindset of "a microwave 'should' cost less than $100," and of a dearth of funding for pathology in general (popular shows like CSI to the contrary). :-) I'd still suggest that $1749 is a heck of a lot better (and a lot less laughable) than the $18,000 or $30,000 that's widely quoted and posted, and it's the exact reason we brought an under-$2000 lab microwave to market in the first place. One could argue just as convincingly against all kinds of specialized equipment or reagents on the basis of cost, not just microwaves. We all know of everything from saliva to cheap rice steamers being used in histo labs, and while they may actually be perfectly serviceable, from the standpoint of repeatability or liability, this kind of thing gives me the willies (and that's a technical term). My yardstick is always "what would I be comfortable with if my kid's diagnosis hung in the balance?" Healthy debate is good! Phil -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Mobile: 860.597.6796 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com Joe Nocito wrote: > ok, but with the budgets today, many people can't afford a $1749 > microwave when they can buy one at Walmart, K-Mart, or somewhere else > for $79. > Not to make you angry or anything, but I'm wondering how long has it > been since you worked in a lab? Histo's budget is the first one cut in > the lab because we are not essential. > I can't count how many times I fought and fought for my budgets. > If I tried to justify a $1749 microwave for special stains, HIER or > whatever, I would have been laughed out the manager's office. > Just my 4 cents. > > JTT > ----- Original Message ----- From: "Phil McArdle" > To: "Kathleen Boozer" > Cc: > Sent: Wednesday, September 05, 2007 9:07 AM > Subject: Re: [Histonet] Microwaves VENDOR RESPONSE > > >> Again, a microwave vendor weighs in (so far I haven't received any >> flames), so read at your own risk. :-) >> >> At the risk of sounding overly and overtly commercial, after reading >> post after post of $30,000+ and $18,000 and similarly high figures for >> lab microwaves, I really feel the need to set the record straight. >> Depending on the usage requirements, we have laboratory microwaves as >> low as $1749 for a "bare bones" model for simple operations, to >> mid-priced units, to under $11,000 for a vacuum equipped microwave >> processor capable of the +/- 0.5 degree C temperature control >> necessary for tissue processing. >> >> (I can feel the heat already!) >> >> There are many compelling reasons to replace a kitchen microwave with >> a lab model; feel free to download, read, and even share with >> colleagues our Microwave Companion at >> >> http://www.ebsciences.com/pdf/EBS_MW_COMPANION.pdf >> >> Best regards, and see you at NSH, >> >> Phil McArdle >> >> -- >> Phil McArdle >> Microwave Product Manager >> >> Energy Beam Sciences, Inc. >> 29-B Kripes Rd. >> East Granby, CT 06026 >> >> Tel: 800.992.9037 x 341 >> Mobile: 860.597.6796 >> Fax: 860.653.0422 >> >> pmcardle@ebsciences.com >> www.ebsciences.com >> >> Kathleen Boozer wrote: >>> What is the best microwave for a small lab using it only for heating >>> Bouin's and Silver Nitrate for special stains? I just can't believe >>> I would have to spend $30,000+ or slow down and use a waterbath. >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> I skate to where the puck is going to be, not to where it's been. >> - Wayne Gretsky >> >> You must be the change you want to see in the world. >> - Mahatma Gandhi >> >> NOTE: This message, together with any attachments, is intended only >> for the use of the individual or entity to which it is addressed and >> may contain information that is legally privileged, confidential and >> exempt from disclosure. If you are not the intended recipient, >> however, there's not a lot I can do about it, and it was probably my >> mistake anyway. So please do the right thing and make this e-mail go >> away. Thank you. >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Mobile: 860.597.6796 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com I skate to where the puck is going to be, not to where it's been. - Wayne Gretsky You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it, and it was probably my mistake anyway. So please do the right thing and make this e-mail go away. Thank you. From Lynn.Burton <@t> Illinois.gov Wed Sep 5 11:20:54 2007 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Wed Sep 5 11:22:38 2007 Subject: [Histonet] Microwaves VENDOR RESPONSE References: <46DD8689.4AA8.00C0.0@ah.org> <46DEB823.4060708@ebsciences.com> <003b01c7efd5$52d0da00$d49eae18@yourxhtr8hvc4p> Message-ID: We purchased a microwave in 1998, still in good working order, from then Shandon for about $600. We thought that was ridiculous at the time. All we use it for is special stains. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Joe Nocito Sent: Wed 9/5/2007 10:56 AM To: Phil McArdle; Kathleen Boozer Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Microwaves VENDOR RESPONSE ok, but with the budgets today, many people can't afford a $1749 microwave when they can buy one at Walmart, K-Mart, or somewhere else for $79. Not to make you angry or anything, but I'm wondering how long has it been since you worked in a lab? Histo's budget is the first one cut in the lab because we are not essential. I can't count how many times I fought and fought for my budgets. If I tried to justify a $1749 microwave for special stains, HIER or whatever, I would have been laughed out the manager's office. Just my 4 cents. JTT ----- Original Message ----- From: "Phil McArdle" To: "Kathleen Boozer" Cc: Sent: Wednesday, September 05, 2007 9:07 AM Subject: Re: [Histonet] Microwaves VENDOR RESPONSE > Again, a microwave vendor weighs in (so far I haven't received any > flames), so read at your own risk. :-) > > At the risk of sounding overly and overtly commercial, after reading post > after post of $30,000+ and $18,000 and similarly high figures for lab > microwaves, I really feel the need to set the record straight. Depending > on the usage requirements, we have laboratory microwaves as low as $1749 > for a "bare bones" model for simple operations, to mid-priced units, to > under $11,000 for a vacuum equipped microwave processor capable of the +/- > 0.5 degree C temperature control necessary for tissue processing. > > (I can feel the heat already!) > > There are many compelling reasons to replace a kitchen microwave with a > lab model; feel free to download, read, and even share with colleagues our > Microwave Companion at > > http://www.ebsciences.com/pdf/EBS_MW_COMPANION.pdf > > Best regards, and see you at NSH, > > Phil McArdle > > -- > Phil McArdle > Microwave Product Manager > > Energy Beam Sciences, Inc. > 29-B Kripes Rd. > East Granby, CT 06026 > > Tel: 800.992.9037 x 341 > Mobile: 860.597.6796 > Fax: 860.653.0422 > > pmcardle@ebsciences.com > www.ebsciences.com > > Kathleen Boozer wrote: >> What is the best microwave for a small lab using it only for heating >> Bouin's and Silver Nitrate for special stains? I just can't believe I >> would have to spend $30,000+ or slow down and use a waterbath. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > I skate to where the puck is going to be, not to where it's been. > - Wayne Gretsky > > You must be the change you want to see in the world. > - Mahatma Gandhi > > NOTE: This message, together with any attachments, is intended only for > the use of the individual or entity to which it is addressed and may > contain information that is legally privileged, confidential and exempt > from disclosure. If you are not the intended recipient, however, there's > not a lot I can do about it, and it was probably my mistake anyway. So > please do the right thing and make this e-mail go away. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charles.Embrey <@t> carle.com Wed Sep 5 11:38:03 2007 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Wed Sep 5 11:41:40 2007 Subject: [Histonet] Microwaves In-Reply-To: <46DDA609.4AA8.00C0.0@ah.org> Message-ID: <44780C571F28624DBB446DE55C4D733A1FE532@EXCHANGEBE1.carle.com> When CAP called me after my inspection the inspector said that "manufacturer's Instructions" don't say food only. He said that it applied to the safety warnings about use. If I hadn't gotten the word directly from CAP I would still be confused but I am still using my kitchen microwave to heat simple reagents and do special stains with their blessing. My first contact from CAP was Ed Gruber and he had me fax my information to Wilson Kung. Mr. Kung can be reached at 800-323-4040 X-7493 Charles Embrey, PA(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathleen Boozer Sent: Tuesday, September 04, 2007 8:38 PM To: JR R; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Microwaves CAP now says "in accordance with manufacturer's instructions". In other words, food only, no chemicals. How do I get away with that? >>> JR R 09/04/2007 16:58 >>> I use a plain old GE turntable microwave for that. It was like $150.00. You do want the turntable kind and with a digital timer is best. What you want to do is test the microwave to see exactly how long it takes to heat your solution to the required temperature in the exact container (staining tub, probably?) that you will be using. Jerry L. Ricks Research Scientist U.W. Medicine at South Lake Union 815 Mercer Street Seattle, WA 98109 (206)-685-7190> Date: Tue, 4 Sep 2007 16:23:38 -0700> From: BoozerKA@ah.org> To: histonet@lists.utsouthwestern.edu> Subject: [Histonet] Microwaves> > What is the best microwave for a small lab using it only for heating Bouin's and Silver Nitrate for special stains? I just can't believe I would have to spend $30,000+ or slow down and use a waterbath.> > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Connect to the next generation of MSN Messenger http://imagine-msn.com/messenger/launch80/default.aspx?locale=en-us&sour ce=wlmailtagline_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Wed Sep 5 11:48:42 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Sep 5 11:48:14 2007 Subject: [Histonet] Microwaves VENDOR RESPONSE References: <46DD8689.4AA8.00C0.0@ah.org> <46DEB823.4060708@ebsciences.com> <003b01c7efd5$52d0da00$d49eae18@yourxhtr8hvc4p> <46DED674.8060704@ebsciences.com> Message-ID: <000d01c7efdc$9fdbe360$d49eae18@yourxhtr8hvc4p> are you sure it's the willies and not the johnnies? Since the magnetron or whatever it was that fried my tissue, I'd wait for the traditional processing. Call me a dinosaur, but I really don't like doing special stains in the microwave. The only thing I use a microwave for at my house is to defrost and reheat stuff (technical term). I'm sure there are people out there who can cook a 6 course gourmet meal. My best friend can process all types of tissue from biopsies to uterus. As a matter of fact, he was there grossing when something went wrong and told me that he's never seen tissue like that before. JTT ----- Original Message ----- From: "Phil McArdle" To: "Joe Nocito" Cc: Sent: Wednesday, September 05, 2007 11:16 AM Subject: Re: [Histonet] Microwaves VENDOR RESPONSE > Hi Joe: > > No argument there. I'm painfully aware of both a mindset of "a microwave > 'should' cost less than $100," and of a dearth of funding for pathology in > general (popular shows like CSI to the contrary). :-) I'd still suggest > that $1749 is a heck of a lot better (and a lot less laughable) than the > $18,000 or $30,000 that's widely quoted and posted, and it's the exact > reason we brought an under-$2000 lab microwave to market in the first > place. > > One could argue just as convincingly against all kinds of specialized > equipment or reagents on the basis of cost, not just microwaves. We all > know of everything from saliva to cheap rice steamers being used in histo > labs, and while they may actually be perfectly serviceable, from the > standpoint of repeatability or liability, this kind of thing gives me the > willies (and that's a technical term). My yardstick is always "what would > I be comfortable with if my kid's diagnosis hung in the balance?" > > Healthy debate is good! > > Phil > -- > Phil McArdle > Microwave Product Manager > > Energy Beam Sciences, Inc. > 29-B Kripes Rd. > East Granby, CT 06026 > > Tel: 800.992.9037 x 341 > Mobile: 860.597.6796 > Fax: 860.653.0422 > > pmcardle@ebsciences.com > www.ebsciences.com > > > > > > > Joe Nocito wrote: >> ok, but with the budgets today, many people can't afford a $1749 >> microwave when they can buy one at Walmart, K-Mart, or somewhere else for >> $79. >> Not to make you angry or anything, but I'm wondering how long has it >> been since you worked in a lab? Histo's budget is the first one cut in >> the lab because we are not essential. >> I can't count how many times I fought and fought for my budgets. >> If I tried to justify a $1749 microwave for special stains, HIER or >> whatever, I would have been laughed out the manager's office. >> Just my 4 cents. >> >> JTT >> ----- Original Message ----- From: "Phil McArdle" >> >> To: "Kathleen Boozer" >> Cc: >> Sent: Wednesday, September 05, 2007 9:07 AM >> Subject: Re: [Histonet] Microwaves VENDOR RESPONSE >> >> >>> Again, a microwave vendor weighs in (so far I haven't received any >>> flames), so read at your own risk. :-) >>> >>> At the risk of sounding overly and overtly commercial, after reading >>> post after post of $30,000+ and $18,000 and similarly high figures for >>> lab microwaves, I really feel the need to set the record straight. >>> Depending on the usage requirements, we have laboratory microwaves as >>> low as $1749 for a "bare bones" model for simple operations, to >>> mid-priced units, to under $11,000 for a vacuum equipped microwave >>> processor capable of the +/- 0.5 degree C temperature control necessary >>> for tissue processing. >>> >>> (I can feel the heat already!) >>> >>> There are many compelling reasons to replace a kitchen microwave with a >>> lab model; feel free to download, read, and even share with colleagues >>> our Microwave Companion at >>> >>> http://www.ebsciences.com/pdf/EBS_MW_COMPANION.pdf >>> >>> Best regards, and see you at NSH, >>> >>> Phil McArdle >>> >>> -- >>> Phil McArdle >>> Microwave Product Manager >>> >>> Energy Beam Sciences, Inc. >>> 29-B Kripes Rd. >>> East Granby, CT 06026 >>> >>> Tel: 800.992.9037 x 341 >>> Mobile: 860.597.6796 >>> Fax: 860.653.0422 >>> >>> pmcardle@ebsciences.com >>> www.ebsciences.com >>> >>> Kathleen Boozer wrote: >>>> What is the best microwave for a small lab using it only for heating >>>> Bouin's and Silver Nitrate for special stains? I just can't believe I >>>> would have to spend $30,000+ or slow down and use a waterbath. >>>> >>>> >>>> _______________________________________________ >>>> Histonet mailing list >>>> Histonet@lists.utsouthwestern.edu >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >>> >>> >>> I skate to where the puck is going to be, not to where it's been. >>> - Wayne Gretsky >>> >>> You must be the change you want to see in the world. >>> - Mahatma Gandhi >>> >>> NOTE: This message, together with any attachments, is intended only for >>> the use of the individual or entity to which it is addressed and may >>> contain information that is legally privileged, confidential and exempt >>> from disclosure. If you are not the intended recipient, however, there's >>> not a lot I can do about it, and it was probably my mistake anyway. So >>> please do the right thing and make this e-mail go away. Thank you. >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > Phil McArdle > Microwave Product Manager > > Energy Beam Sciences, Inc. > 29-B Kripes Rd. > East Granby, CT 06026 > > Tel: 800.992.9037 x 341 > Mobile: 860.597.6796 > Fax: 860.653.0422 > > pmcardle@ebsciences.com > www.ebsciences.com > > I skate to where the puck is going to be, not to where it's been. > - Wayne Gretsky > > You must be the change you want to see in the world. > - Mahatma Gandhi > > NOTE: This message, together with any attachments, is intended only for > the use of the individual or entity to which it is addressed and may > contain information that is legally privileged, confidential and exempt > from disclosure. If you are not the intended recipient, however, there's > not a lot I can do about it, and it was probably my mistake anyway. So > please do the right thing and make this e-mail go away. Thank you. From themagoos <@t> rushmore.com Wed Sep 5 12:21:51 2007 From: themagoos <@t> rushmore.com (themagoos) Date: Wed Sep 5 12:22:02 2007 Subject: [Histonet] Xylene Message-ID: <46dee5af.14b.450a.1030817715@rushmore.com> Can somebody tell me if there are any effects on tissue if there is prolonged processing time in xylene? Jason McGough HT(ASCP) Clinical Laboratory of the Black Hills Account Representative - Anatomic Pathology 2805 5th Street Rapid City, SD 57701 605-343-2267 jmcgough@clinlab.com From Melissa.Gonzalez <@t> cellgenesys.com Wed Sep 5 13:30:38 2007 From: Melissa.Gonzalez <@t> cellgenesys.com (Melissa Gonzalez) Date: Wed Sep 5 13:30:51 2007 Subject: [Histonet] RE:The great apoptosis stains debate (TUNEL, Caspases, etc) In-Reply-To: <68e5ia$flbq@mail.cellgenesys.com> References: <68e5ia$flbq@mail.cellgenesys.com> Message-ID: <2884B897182A1D438C7BA24B9A8F94A20E701D@hqsvr01mail.cgi.com> Hi all, I have been around this mess a few years back, I gave up on TUNEL long ago for the various reasons mentioned on the list recently. Per investigators requests, I have tried several ways to demonstrate cell death, however methods such as PI or Annexin staining are more suitable for whole cells, not tissue sections. I have also not had any luck staining for Cytochrome C. We routinely use R&D Systems rabbit anti human/mouse active Caspase3 using a high pH (EDTA) based Ag retrieval in a steamer (enzymes do not work). Works like a charm. Any other suggestions? I know this topic has been thrown around a lot, but these discussions after all, help us get our jobs done more proficiently. Melissa Melissa A. Gonz?lez Edick R&D, Cell Genesys Inc. 500 Forbes Blvd South San Francisco, CA 94080 p(650) 266-3168 f (650) 266-3080 "It's not enough to believe what you see, you must also understand what you see." -Leonardo Da Vinci ------------------------------ Message: 6 Date: Tue, 4 Sep 2007 15:18:39 -0400 From: Cheryl Cross Subject: Re: [Histonet] TUNEL, Formalin, DNA strand breaks To: JR R Cc: histonet@lists.utsouthwestern.edu Message-ID: <4C1E074D-064A-45D1-BD32-13D7E2222B26@aol.com> Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed Hi all - I have been round and round with this issue myself; I was basically told by people who've attempted it that TUNEL on FFPE sections is not specific enough due to the formalin issue. If you are trying to nail apoptosis, what about anti-active caspase-3? mind you, that staining can be a bit of a booger too, but it should be more sensitive and specific for apoptosis (no references to offer, i'm just repeating what i have been told). Cheryl Cross, DVM, Dipl. ACVP Researcher University Corporation for Atmospheric Research College of Veterinary Medicine University of Tennessee Department of Pathology 2407 River Drive, Room A201 Knoxville, TN 37996-4542 (423) 967-2724 fax: 865-974-5616 ccross@ucar.edu ------------------------------ Message: 5 Hi Ray, Sheesh-- I read (and then lost) the paper maybe 5-7 years ago, back when I was tearing my hair out over high background, or false positives for TUNEL stain in formalin fixed, paraffin embedded arterial sections. I can troubleshoot any immunostain, but the TUNEL and ISEL assays bedeviled me. The paper was titled something to the effect of "formaldehyde causes single and double stranded DNA breaks," and was pretty emphatic. I'll look for the article. I think the assay could work in whole cells for flow, or maybe in frozen tissue. My hunch at this point is that this is one of those rare cases where lots and lots of peer reviewed articles are just plain wrong. Hey, if someone has a good protocol, I'd be willing to try it out, and I would be delighted if I turn out to be mistaken. For now, I think TUNEL is the assay of the beast. Oh, here is a thought--say you are looking at a 5 micron section through a nucleus. The microtome blade pretty much had to create a lot of double stranded DNA breaks, no? Jerry L. Ricks Research Scientist U.W. Medicine at South Lake Union 815 Mercer Street Seattle, WA 98109 (206)-685-7190 From: koellingr@comcast.netTo: rosenfeldtek@hotmail.com; histonet@lists.utsouthwestern.eduSubject: RE: [Histonet] TUNELDate: Fri, 31 Aug 2007 23:17:03 +0000 Jerry, Could you expand on or give references to formalin causing DNA strand breaks? Double strand breaks, single strand, blunt end, overhanging? My pile of papers and having done TUNEL for years says that formalin fixation is a very good technique for TUNEL and many peer-reviewed articles in which TUNEL is used as a technique, use formalin fixation and how can that be if formalin is causing strand breaks? In fact one paper I'm looking at says that extended (5-7 weeks in formalin) fixation causes loss of TUNEL signal. If formalin is causing breaks, you would assume that TUNEL pos signals would increase with extended formalin fixation. Even the use of the monoclonal antibody F7-26, for single stranded DNA, touts formalin fixation for their claims of discriminating apoptosis from necrosis. The question asks about extended alcohol fixation but your answer is possibly a lot of false positives because formalin causes DNA breaks. Does this imply that alcohol won't? Have done a lot of TUNEL on alcohol fixed samples. True I couldn't pretreat them and handle them they way I would handle FFPE tissue. Also true that we could argue specificity and ability or not to discriminate apoptosis from necrosis for quite a while. But if formalin itself is causing the breaks in DNA, I and a lot of people are in big trouble with our science projects and experiments. Also I can't envision why 2 cells are showing TUNEL positivity while 2 of the same type of cells right next to them (and getting the same formalin fix), are absolutely clean and there is no background? Thanks for any information you can provide. Ray Koelling PhenoPath Laboratories Seattle, WA From RSRICHMOND <@t> aol.com Wed Sep 5 13:56:14 2007 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Wed Sep 5 13:56:25 2007 Subject: [Histonet] Re: RDO decalcifier Message-ID: I don't see how your hazmats people can tell you what to do to dispose of RDO, since nobody has any idea what's in it. It's a murky yellow liquid that continuously throws a black sediment that has to be filtered out if you're to see your specimen in it. It's sort of the ultimate secret formula, and I've always wondered why it's so popular - I've had to use it many different places in my travels. I'd suggest either an ordinary proprietary decalcifier that's a clear liquid, probably hydrochloric acid - or else save money and dilute your own hydrochloric acid. You can re-use it for a while. When the time comes to dispose of it, it can be safely diluted with a lot of water and put down the drain. Remember - it's been stressed on this list many times - that "Decal" is a brand name still in use, and that other brands of decalcifying solution should not be called Decal. Bob Richmond Samurai Pathologist (never a decalcified Carmelite) Knoxville TN From carl.hobbs <@t> kcl.ac.uk Wed Sep 5 14:29:36 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Wed Sep 5 14:30:13 2007 Subject: [Histonet] IHC anti-cleaved caspase 3 Message-ID: <001801c7eff3$18894850$4101a8c0@carlba65530bda> Hi. Recent Cleaved caspase 3 posts have not mentioned the antibody details: I would be grateful for the source of these Abs. Carl From melissah <@t> uchicago.edu Wed Sep 5 14:36:50 2007 From: melissah <@t> uchicago.edu (melissah rowe) Date: Wed Sep 5 14:37:02 2007 Subject: [Histonet] avian sperm cell topography Message-ID: Hi, I am a PhD student working on a behavioral ecology project on Australian fairy-wrens. I am currently trying to find out if I can determine the dimensions of discrete sections of individual sperm cells, i.e. acrosome length, nucleus length, midpiece length and tail length using the material I currently have available. I have prepared slide smears of sperm that has been previously stained with an eosin- nigrosin stain (to determine cell viability) and would like to be able to somehow stain different segments of the sperm so that I can identify and measure them using an ocular micrometer on a bright field or phase contrast microscope set up. It is my understanding that I cannot use fluorescent stains given that I have already used a colormetric stain (the eosin-nigrosin). If anyone has suggestions for ways to identify discrete regions of avian (passerine) sperm cells I would be very grateful to hear from you. Many thanks in advance, melissah ----- melissah rowe PhD candidate Department of Ecology & Evolution University of Chicago E. 57th Street, Chicago, IL, 60637 ph: +1 773-702-3070 fax: + 1 773-702-9740 email: melissah@uchicago.edu From tp2 <@t> medicine.wisc.edu Wed Sep 5 14:39:04 2007 From: tp2 <@t> medicine.wisc.edu (Thomas Pier) Date: Wed Sep 5 14:39:41 2007 Subject: [Histonet] IHC anti-cleaved caspase 3 Message-ID: <46DEBF88020000DF0000A736@gwmail.medicine.wisc.edu> Cell Signalling Technology has a good rabbit monoclonal for Cleaved Caspase-3. Tom Pier >>> "Carl Hobbs" 09/05/07 2:29 PM >>> Hi. Recent Cleaved caspase 3 posts have not mentioned the antibody details: I would be grateful for the source of these Abs. Carl _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ccross6032 <@t> aol.com Wed Sep 5 14:54:35 2007 From: ccross6032 <@t> aol.com (Cheryl Cross) Date: Wed Sep 5 14:54:56 2007 Subject: [Histonet] IHC anti-cleaved caspase 3 In-Reply-To: <46DEBF88020000DF0000A736@gwmail.medicine.wisc.edu> References: <46DEBF88020000DF0000A736@gwmail.medicine.wisc.edu> Message-ID: I will second the Cell Signaling antibody - we have tried antibodies from Promega (which gave tons of background staining)...then via this site i was pointed to Cell Signaling's monoclonal which we tried; the polyclonal actually has been working very well with minimal background (we were getting lots of respiratory epithelium and endothelium lighting up in control animals). I have been using it in mice with this protocol: EDTA with PASCAL primary antibody 60 minutes, dilution 1:125 Rabbit envision DAB plus Hope this helps! Cheryl Cross, DVM, Dipl. ACVP Researcher University Corporation for Atmospheric Research College of Veterinary Medicine University of Tennessee Department of Pathology 2407 River Drive, Room A201 Knoxville, TN 37996-4542 (423) 967-2724 fax: 865-974-5616 ccross@ucar.edu > From bakevictoria <@t> gmail.com Wed Sep 5 14:59:45 2007 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Wed Sep 5 14:59:56 2007 Subject: [Histonet] Fwd: Users of Lab Vision autostainer In-Reply-To: <4f016b690709050602w54725be5ob99e25453ed48bef@mail.gmail.com> References: <4f016b690709050602w54725be5ob99e25453ed48bef@mail.gmail.com> Message-ID: <4f016b690709051259rcdffccet70d7f97ae0457707@mail.gmail.com> One more try, first one didn't go through it seems! Thanks ---------- Forwarded message ---------- From: Victoria Baker Date: Sep 5, 2007 9:02 AM Subject: Users of Lab Vision autostainer To: Histo Net list server Hi I'm a new user of the Lab Vision autostainer and I'm looking to see if I can find users in Histo-land that have experience with it. The facility only works with human tissue and all of the antibodies are for dx purposes. My key questions are as follows: How many antibodies is your lab running? How many users do you allow? How many people do you allow programming rights and at what level of supervision are they? How many of these antibodies are from Lab Vision? a) are they concentrates or pre-dilutes? b) for HIER are you using their PT modules/procedures or your own equipment (microwave, steamer, pressure cooker etc) and in house designed protocols for retrieval? c) for digestion do you only use their Pro-K or have you designed your own in-house methods using other reagents for digestion? d) do you put your controls on the same slide as the patient? e) are your controls in-house or commercial? f) do you have more than one stainer hooked up to one computer system? What Version of software do you currently have on your system? Any feed back would be very helpful. Thanks in advance. Vikki Baker Interim Histology Manager Mission Hospital System Asheville, NC From PMcArdle <@t> ebsciences.com Wed Sep 5 15:00:47 2007 From: PMcArdle <@t> ebsciences.com (Phil McArdle) Date: Wed Sep 5 15:01:07 2007 Subject: [Histonet] Microwaves VENDOR RESPONSE In-Reply-To: <000d01c7efdc$9fdbe360$d49eae18@yourxhtr8hvc4p> References: <46DD8689.4AA8.00C0.0@ah.org> <46DEB823.4060708@ebsciences.com> <003b01c7efd5$52d0da00$d49eae18@yourxhtr8hvc4p> <46DED674.8060704@ebsciences.com> <000d01c7efdc$9fdbe360$d49eae18@yourxhtr8hvc4p> Message-ID: <46DF0AEF.7030203@ebsciences.com> Hi Joe: Obviously, a microwave vendor hates to hear microwave horror stories, so again, no argument - even though I'm not privy to details of the fried biopsies in question or what type/vintage of microwave, anyone who's experienced a malfunction involving patient samples doesn't want a repeat performance. And pathology is, must be, risk averse. That said, again, any mechanical or electronic equipment can fail, or user error can contribute; just look at the "hang-up" problems with older tissue processors that are now ancient history. It's up to manufacturers to minimize the possibilities of failure, since patient care is at stake. Improvement is therefore a continual, ongoing process. For example, while for years EBS microwave processors incorporated safety shutdown modes in the event of vent failure, probe failure (open and closed) and many other component-related issues, about two years ago we determined that the microwave did not have a comprehensive set of safeguards to deal with user errors, for example, temperature overshoots caused by too small a container for a given power setting, or failure to place the temperature probe in solution. So we developed multiple safety mechanisms to head off user errors of this sort. PMM -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Mobile: 860.597.6796 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com Joe Nocito wrote: > are you sure it's the willies and not the johnnies? > Since the magnetron or whatever it was that fried my tissue, I'd wait > for the traditional processing. Call me a dinosaur, but I really don't > like doing special stains in the microwave. The only thing I use a > microwave for at my house is to defrost and reheat stuff (technical > term). I'm sure there are people out there who can cook a 6 course > gourmet meal. My best friend can process all types of tissue from > biopsies to uterus. > As a matter of fact, he was there grossing when something went wrong > and told me that he's never seen tissue like that before. > > JTT > ----- Original Message ----- From: "Phil McArdle" > To: "Joe Nocito" > Cc: > Sent: Wednesday, September 05, 2007 11:16 AM > Subject: Re: [Histonet] Microwaves VENDOR RESPONSE > > >> Hi Joe: >> >> No argument there. I'm painfully aware of both a mindset of "a >> microwave 'should' cost less than $100," and of a dearth of funding >> for pathology in general (popular shows like CSI to the contrary). :-) >> I'd still suggest that $1749 is a heck of a lot better (and a lot less >> laughable) than the $18,000 or $30,000 that's widely quoted and >> posted, and it's the exact reason we brought an under-$2000 lab >> microwave to market in the first place. >> >> One could argue just as convincingly against all kinds of specialized >> equipment or reagents on the basis of cost, not just microwaves. We >> all know of everything from saliva to cheap rice steamers being used >> in histo labs, and while they may actually be perfectly serviceable, >> from the standpoint of repeatability or liability, this kind of thing >> gives me the willies (and that's a technical term). My yardstick is >> always "what would I be comfortable with if my kid's diagnosis hung in >> the balance?" >> >> Healthy debate is good! >> >> Phil >> -- >> Phil McArdle >> Microwave Product Manager >> >> Energy Beam Sciences, Inc. >> 29-B Kripes Rd. >> East Granby, CT 06026 >> >> Tel: 800.992.9037 x 341 >> Mobile: 860.597.6796 >> Fax: 860.653.0422 >> >> pmcardle@ebsciences.com >> www.ebsciences.com >> >> >> >> >> >> >> Joe Nocito wrote: >>> ok, but with the budgets today, many people can't afford a $1749 >>> microwave when they can buy one at Walmart, K-Mart, or somewhere else >>> for $79. >>> Not to make you angry or anything, but I'm wondering how long has >>> it been since you worked in a lab? Histo's budget is the first one >>> cut in the lab because we are not essential. >>> I can't count how many times I fought and fought for my budgets. >>> If I tried to justify a $1749 microwave for special stains, HIER >>> or whatever, I would have been laughed out the manager's office. >>> Just my 4 cents. >>> >>> JTT >>> ----- Original Message ----- From: "Phil McArdle" >>> >>> To: "Kathleen Boozer" >>> Cc: >>> Sent: Wednesday, September 05, 2007 9:07 AM >>> Subject: Re: [Histonet] Microwaves VENDOR RESPONSE >>> >>> >>>> Again, a microwave vendor weighs in (so far I haven't received any >>>> flames), so read at your own risk. :-) >>>> >>>> At the risk of sounding overly and overtly commercial, after reading >>>> post after post of $30,000+ and $18,000 and similarly high figures >>>> for lab microwaves, I really feel the need to set the record >>>> straight. Depending on the usage requirements, we have laboratory >>>> microwaves as low as $1749 for a "bare bones" model for simple >>>> operations, to mid-priced units, to under $11,000 for a vacuum >>>> equipped microwave processor capable of the +/- 0.5 degree C >>>> temperature control necessary for tissue processing. >>>> >>>> (I can feel the heat already!) >>>> >>>> There are many compelling reasons to replace a kitchen microwave >>>> with a lab model; feel free to download, read, and even share with >>>> colleagues our Microwave Companion at >>>> >>>> http://www.ebsciences.com/pdf/EBS_MW_COMPANION.pdf >>>> >>>> Best regards, and see you at NSH, >>>> >>>> Phil McArdle >>>> >>>> -- >>>> Phil McArdle >>>> Microwave Product Manager >>>> >>>> Energy Beam Sciences, Inc. >>>> 29-B Kripes Rd. >>>> East Granby, CT 06026 >>>> >>>> Tel: 800.992.9037 x 341 >>>> Mobile: 860.597.6796 >>>> Fax: 860.653.0422 >>>> >>>> pmcardle@ebsciences.com >>>> www.ebsciences.com >>>> >>>> Kathleen Boozer wrote: >>>>> What is the best microwave for a small lab using it only for >>>>> heating Bouin's and Silver Nitrate for special stains? I just >>>>> can't believe I would have to spend $30,000+ or slow down and use a >>>>> waterbath. >>>>> >>>>> >>>>> _______________________________________________ >>>>> Histonet mailing list >>>>> Histonet@lists.utsouthwestern.edu >>>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>>> >>>> >>>> >>>> >>>> I skate to where the puck is going to be, not to where it's been. >>>> - Wayne Gretsky >>>> >>>> You must be the change you want to see in the world. >>>> - Mahatma Gandhi >>>> >>>> NOTE: This message, together with any attachments, is intended only >>>> for the use of the individual or entity to which it is addressed and >>>> may contain information that is legally privileged, confidential and >>>> exempt from disclosure. If you are not the intended recipient, >>>> however, there's not a lot I can do about it, and it was probably my >>>> mistake anyway. So please do the right thing and make this e-mail go >>>> away. Thank you. >>>> >>>> _______________________________________________ >>>> Histonet mailing list >>>> Histonet@lists.utsouthwestern.edu >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> -- >> Phil McArdle >> Microwave Product Manager >> >> Energy Beam Sciences, Inc. >> 29-B Kripes Rd. >> East Granby, CT 06026 >> >> Tel: 800.992.9037 x 341 >> Mobile: 860.597.6796 >> Fax: 860.653.0422 >> >> pmcardle@ebsciences.com >> www.ebsciences.com >> >> I skate to where the puck is going to be, not to where it's been. >> - Wayne Gretsky >> >> You must be the change you want to see in the world. >> - Mahatma Gandhi >> >> NOTE: This message, together with any attachments, is intended only >> for the use of the individual or entity to which it is addressed and >> may contain information that is legally privileged, confidential and >> exempt from disclosure. If you are not the intended recipient, >> however, there's not a lot I can do about it, and it was probably my >> mistake anyway. So please do the right thing and make this e-mail go >> away. Thank you. > I skate to where the puck is going to be, not to where it's been. - Wayne Gretsky You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it, and it was probably my mistake anyway. So please do the right thing and make this e-mail go away. Thank you. From rjbuesa <@t> yahoo.com Wed Sep 5 15:01:21 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 5 15:01:34 2007 Subject: [Histonet] Xylene In-Reply-To: <46dee5af.14b.450a.1030817715@rushmore.com> Message-ID: <329330.36844.qm@web61219.mail.yahoo.com> Tissues usually become brittle, which difficulties sectioning. Ren? J. themagoos wrote: Can somebody tell me if there are any effects on tissue if there is prolonged processing time in xylene? Jason McGough HT(ASCP) Clinical Laboratory of the Black Hills Account Representative - Anatomic Pathology 2805 5th Street Rapid City, SD 57701 605-343-2267 jmcgough@clinlab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Boardwalk for $500? In 2007? Ha! Play Monopoly Here and Now (it's updated for today's economy) at Yahoo! Games. From liz <@t> premierlab.com Wed Sep 5 15:09:02 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Sep 5 15:12:27 2007 Subject: [Histonet] IHC anti-cleaved caspase 3 In-Reply-To: <94544618683B4453954E6A65AF34BC2E@PremierLab.local> References: <46DEBF88020000DF0000A736@gwmail.medicine.wisc.edu> <94544618683B4453954E6A65AF34BC2E@PremierLab.local> Message-ID: I like the cell signaling antibody also, I tried biocare's but did not = have much success with it. The cell signaling antibody also works with = pronase digestion, as well as the EDTA pH9 HIER. We have even used it = on bone sections with the pronase digestion. Works in multiple species, = I have used it on human, rat, mouse, guinea pig, porcine and canine. = Our protocol is similar to Cheryl's. It=92s a bit pricy as antibodies = go, but I feel its worth it. Good lot to lot consistency. We have = probably gone through about 5 different lots, with not changing the = protocol at all. I have a written protocol if anyone is interested. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com =20 Ship to Address: =20 Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu = [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl = Cross Sent: Wednesday, September 05, 2007 2:01 PM To: Thomas Pier Cc: carl.hobbs@kcl.ac.uk; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC anti-cleaved caspase 3 I will second the Cell Signaling antibody - we have tried antibodies = from Promega (which gave tons of background staining)...then via this = site i was pointed to Cell Signaling's monoclonal which we tried; the = polyclonal actually has been working very well with minimal background = (we were getting lots of respiratory epithelium and endothelium lighting = up in control animals). I have been using it in mice with this protocol: EDTA with PASCAL primary antibody 60 minutes, dilution 1:125 Rabbit envision DAB plus Hope this helps! Cheryl Cross, DVM, Dipl. ACVP Researcher University Corporation for Atmospheric Research College of Veterinary = Medicine University of Tennessee Department of Pathology 2407 River Drive, Room A201 Knoxville, TN 37996-4542 (423) 967-2724 fax: 865-974-5616 ccross@ucar.edu > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG Free Edition.=20 Version: 7.5.485 / Virus Database: 269.13.5/990 - Release Date: 9/4/2007 = 10:36 PM =20 No virus found in this outgoing message. Checked by AVG Free Edition.=20 Version: 7.5.485 / Virus Database: 269.13.5/990 - Release Date: 9/4/2007 = 10:36 PM =20 From soper <@t> ciwemb.edu Wed Sep 5 15:13:36 2007 From: soper <@t> ciwemb.edu (Sarah Clatterbuck Soper) Date: Wed Sep 5 15:13:58 2007 Subject: [Histonet] Freezing mouse testes for frozen sections Message-ID: <46DF0DF0.2030306@ciwemb.edu> Hi all, I've started attempting to section unfixed frozen mouse testes in order to placate a specific antibody we have to use. I am new to frozen sections and I'm having trouble with the testes cracking when I freeze them. I've tried both freezing in isopentane cooled on liquid nitrogen and an acetone/dry ice slurry. Either way the testes crack, usually one big crack end to end. Doesn't seem to be as much of a problem with our mutant testes, which are about 1/3 the size of wild-type. I wish I could just trim the wild-type down to a smaller size, but obviously that's not an option! Any recommendations? Thanks so much! Sarah From mpence <@t> grhs.net Wed Sep 5 15:49:48 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Sep 5 15:49:59 2007 Subject: [Histonet] Olympus BX40 Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C701@IS-E2K3.grhs.net> Need some help, I am looking for a 60x objective for an Olympus microscope BX40. Would anyone know where I might get a used one or if they even make one this size for this scope? Thanks, Mike From YuJ2 <@t> upmc.edu Wed Sep 5 15:58:12 2007 From: YuJ2 <@t> upmc.edu (Yu, Jian) Date: Wed Sep 5 15:58:25 2007 Subject: [Histonet] Look for a used stereoscope In-Reply-To: <661949901A768E4F9CC16D8AF8F2838CA1C701@IS-E2K3.grhs.net> References: <661949901A768E4F9CC16D8AF8F2838CA1C701@IS-E2K3.grhs.net> Message-ID: <7E0A77BFEB9A1E47A63F978E7116821F07986D6D@1upmc-msx11.acct.upmchs.net> Does anyone know a good place to get a used stereoscope? I plan to use it to examine intestinal tumors in mice. Thanks a lot for your information. ******************************************************************* Jian Yu, Ph.D. University of Pittsburgh Cancer Institute Hillman Cancer Center Research Pavilion Office Suite 2.26h 5117 Centre Avenue, Pittsburgh, PA 15213 ******************************************************************* From AnthonyH <@t> chw.edu.au Wed Sep 5 18:44:45 2007 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Sep 5 18:45:36 2007 Subject: [Histonet] RDO decal Message-ID: There is also the issue of EDTA, which is common in many RDO formulations. Checking one MSDS for EDTA reveals: Ecological Information Environmental Fate: When released into the soil, this material is expected to leach into groundwater. When released into the soil, this material may biodegrade to a moderate extent. When released into the soil, this material is not expected to evaporate significantly. When released into water, this material is not expected to evaporate significantly. This material is not expected to significantly bioaccumulate. When released into the air, this material is expected to be readily degraded by photolysis. Environmental Toxicity: This material is not expected to be toxic to aquatic life. The LC50/96-hour values for fish are over 100 mg/l. So neutralisation may not be required. If anyone has info to the contrary please advise. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, 6 September 2007 1:07 AM To: RENEE FISHER; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RDO decal Ren?e: I have not heard of neutralizing RDO, but it would make sense if you want to be "gentle on your sewer system" BUT prepare to a large emission of carbon dioxide when attempting to neutralize it with baking soda. RDO + baking soda (or sodium bicarbonate) will produce water, salt with the acid in RDO (probably sodium chloride or common salt), and carbon dioxide in stoichiometrical amounts (1 CO2 per every 1 NaCl). Therefore that neutralization has to take place in a fumes hood, and you will have to decide which is worst: delivering acid to the sewer system, or carbon dioxide (the Greenhouse gas per excellence) to the atmosphere. It will be "your call". Ren? J. RENEE FISHER wrote: Has anyone heard of neutralizing RDO with baking soda to P.H. 7.0. Our Histo lab had a hazardous waste assessment, and the consultant suggested we not throw the RDO down the drain but that we either collect it for waste removal or neutralize it with baking soda to p.h. 7.0. I have not heard of doing this and do not know of any procedure for it, everyone, anyone's help will be greatly appreciated. Thanks, Renee' _______________________________________________________________________________________ This email may contain confidential protected health information and/or proprietary information belonging to the sender that is legally privileged under local, state, or federal law. This information is intended only for the use of the individual or individuals who have received this. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for the disposal of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: mail, news, photos & more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From cytologer <@t> msn.com Wed Sep 5 21:23:00 2007 From: cytologer <@t> msn.com (ChoiUl Soo) Date: Wed Sep 5 21:23:14 2007 Subject: [Histonet] PTH antibody reacted with dog tissue... Message-ID: Hi Histonetters, I am interested in anti PTH antibody reacted with dog tissue. If anyone has successful experience with any PTH antibody with dog tissue, please tell me one. I have searched through the internet, and results came back with abcam and SantaCruz PTH antibodies predicted to react with them. But they are not sure on it, just predicted on the basis of sequence homology. (abcam say 94% homology with human) I don't have good exprience with Santa Cruz, and never used one by Abcam. Should I rely on it, or find another one? Let me hear your experience. I would appreciate your advice or comment on this. Thank you~. Ul Soo Choi, DVM, PhDKRF priority zoonotic disease research institute, College of Veterinary Medicine, Seoul National University, Shilim9 dong, Gwanakgu, Seoul, Korea 151-742Tel. 82-02-880-8688 Mobile. 82-016-9228-8634Fax. 82-02-880-8662 _________________________________________________________________ ?? ??? ??, Windows Live Space! http://www.spaces.live.com From mmccoy100 <@t> gmail.com Thu Sep 6 00:57:04 2007 From: mmccoy100 <@t> gmail.com (Michelle McCoy) Date: Thu Sep 6 00:57:17 2007 Subject: [Histonet] Floater sources Message-ID: <25355ef80709052257i32794198gd5a64b94758cc1ea@mail.gmail.com> I was reading some of the old archived messages on floaters, and being somewhat new to the field of histotechnology was curious about how floaters can be attributed to a particular tech who performed work on the block. For example, couldn't other sources of contamination be from the automatic stainer, processor carryover (cassette not completely closed/or if closed, friable tissue through the slats eg if sponge not used on larger specimen). In some previous labs I've worked in- floaters were quickly attributed to the cutter, embedder or grosser and might result in a "write up". If the floater is seen in the block how can you differentiate if it came from the grosser carryover/embedder carryover/processing carryover/unsigned re-embedder/or even possibly even client carry over between patients or different specimen types of the same patient. And if it is not in the block --differentiating between the cutter/automatic stainer (I've seen specks of tissue debris in automatic stainers/ sections falling off the slides and into the reagents etc). Obviously all should be done to minimize the factors, but I'm just not clear how a single source is pinpointed and potentially blamed for the event (depending on the lab policy). Thanks for any ideas on this. From Kemlo.Rogerson <@t> waht.swest.nhs.uk Thu Sep 6 02:07:38 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Sep 6 02:07:51 2007 Subject: [Histonet] Xylene Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222EC6C@wahtntex2.waht.swest.nhs.uk> Can somebody tell me if there are any effects on tissue if there is prolonged processing time in xylene? Jason McGough HT(ASCP) Clinical Laboratory of the Black Hills Account Representative - Anatomic Pathology 2805 5th Street Rapid City, SD 57701 605-343-2267 jmcgough@clinlab.com Classically it is said to harden, plus more lipids could be removed. Personally I'm equivocal about that thought; if properly fixed prior to processing I would have thought the hardening effects of xylene were much less than that of the coagulant fixative ethanol. If you do have hard tissue then there is a restorative fluid one can use which I think has oil of cedarwood in it but I don't know the formula off hand. I know it works cos when I was a pup I used it sometimes. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Sunshine is delicious, rain is refreshing, wind braces us up, snow is exhilarating; there is really no such thing as bad weather, only different kinds of good weather. --John Ruskin This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Kemlo.Rogerson <@t> waht.swest.nhs.uk Thu Sep 6 02:17:36 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Sep 6 02:17:48 2007 Subject: [Histonet] Floater sources Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222EC6D@wahtntex2.waht.swest.nhs.uk> I was reading some of the old archived messages on floaters, and being somewhat new to the field of histotechnology was curious about how floaters can be attributed to a particular tech who performed work on the block. For example, couldn't other sources of contamination be from the automatic stainer, processor carryover (cassette not completely closed/or if closed, friable tissue through the slats eg if sponge not used on larger specimen). In some previous labs I've worked in- floaters were quickly attributed to the cutter, embedder or grosser and might result in a "write up". If the floater is seen in the block how can you differentiate if it came from the grosser carryover/embedder carryover/processing carryover/unsigned re-embedder/or even possibly even client carry over between patients or different specimen types of the same patient. And if it is not in the block --differentiating between the cutter/automatic stainer (I've seen specks of tissue debris in automatic stainers/ sections falling off the slides and into the reagents etc). Obviously all should be done to minimize the factors, but I'm just not clear how a single source is pinpointed and potentially blamed for the event (depending on the lab policy). Thanks for any ideas on this. You are exactly correct, floaters can be attributed to a variety of causes, processing machines that aren't regularly changed, transfer on the cutting up forceps, on the waterbath and on the forceps of the embedder. If the floater was from a block that was cut, embedded, cut up before the section with the floater then the culprit is obvious. If the section was cut by someone who didn't cut the block from which the floater floated, then the culprit is the embedder, machine,or cutter up. Realisticaly I would have thought that most floaters would be from the embedder, cutter up, then sectioner as the latter has the greater likelihood of seeing the error of his/ her ways. Waxy forceps of messy forceps, in my experience are the usual culprit but in some instances, necrotic tumours can shed cells in the processor and even the stainer. Floaters are usually obvious as they tend to be at a different level than the tissue section and I'm afraid they are a fact of life. You can reduce the incidence but sadly never eradicate them. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Sunshine is delicious, rain is refreshing, wind braces us up, snow is exhilarating; there is really no such thing as bad weather, only different kinds of good weather. --John Ruskin This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From bakevictoria <@t> gmail.com Thu Sep 6 03:18:04 2007 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Thu Sep 6 03:25:24 2007 Subject: [Histonet] Fwd: Users of Lab Vision autostainer In-Reply-To: <4f016b690709050602w54725be5ob99e25453ed48bef@mail.gmail.com> References: <4f016b690709050602w54725be5ob99e25453ed48bef@mail.gmail.com> Message-ID: <4f016b690709060118t532d22c1if250116e0a6c9af1@mail.gmail.com> ---------- Forwarded message ---------- From: Victoria Baker Date: Sep 5, 2007 9:02 AM Subject: Users of Lab Vision autostainer To: Histo Net list server Hi I'm a new user of the Lab Vision autostainer and I'm looking to see if I can find users in Histo-land that have experience with it. The facility only works with human tissue and all of the antibodies are for dx purposes. My key questions are as follows: How many antibodies is your lab running? How many users do you allow? How many people do you allow programming rights and at what level of supervision are they? How many of these antibodies are from Lab Vision? a) are they concentrates or pre-dilutes? b) for HIER are you using their PT modules/procedures or your own equipment (microwave, steamer, pressure cooker etc) and in house designed protocols for retrieval? c) for digestion do you only use their Pro-K or have you designed your own in-house methods using other reagents for digestion? d) do you put your controls on the same slide as the patient? e) are your controls in-house or commercial? f) do you have more than one stainer hooked up to one computer system? What Version of software do you currently have on your system? Any feed back would be very helpful. Thanks in advance. Vikki Baker Interim Histology Manager Mission Hospital System Asheville, NC From drkwolfe <@t> telus.net Thu Sep 6 03:28:57 2007 From: drkwolfe <@t> telus.net (Joseph Kapler) Date: Thu Sep 6 03:29:28 2007 Subject: [Histonet] Xylene In-Reply-To: <329330.36844.qm@web61219.mail.yahoo.com> Message-ID: Tissues processed in Xylene (especially over long periods of time) the tissue becomes very brittle. Hope that is the response you were looking for. Joseph "DarkWolfe" Kapler I'm an outsider outside of everything I'm an outsider outside of everything I'm an outsider outside of everything Everything you know. Everything you know It disturbs me so -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Wednesday, September 05, 2007 14:01 To: themagoos@rushmore.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Xylene Tissues usually become brittle, which difficulties sectioning. Ren? J. themagoos wrote: Can somebody tell me if there are any effects on tissue if there is prolonged processing time in xylene? Jason McGough HT(ASCP) Clinical Laboratory of the Black Hills Account Representative - Anatomic Pathology 2805 5th Street Rapid City, SD 57701 605-343-2267 jmcgough@clinlab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Boardwalk for $500? In 2007? Ha! Play Monopoly Here and Now (it's updated for today's economy) at Yahoo! Games. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.5.485 / Virus Database: 269.13.5/988 - Release Date: 9/4/2007 09:14 No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.5.485 / Virus Database: 269.13.5/988 - Release Date: 9/4/2007 09:14 From Jacqueline.Malam <@t> rli.mbht.nhs.uk Thu Sep 6 03:59:13 2007 From: Jacqueline.Malam <@t> rli.mbht.nhs.uk (Malam Jacqueline) Date: Thu Sep 6 04:07:18 2007 Subject: [Histonet] Unsubscribe Message-ID: Please would you unsubscribe me as I am retiring tomorrow - thanks Jacqui malam Lancaster uk DISCLAIMER: This e-mail is confidential and privileged. If you are not the intended recipient please accept our apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform postmaster@rli.mbht.nhs.uk that this message has gone astray before deleting it. Comments or opinions expressed in this email are those of their respective contributors only. The views expressed do not represent the views of the Trust, its management or employees. University Hospitals of Morecambe Bay NHS Trust is not responsible and disclaims any and all liability for the content of comments written within.Thank you for your co-operation. From jnocito <@t> satx.rr.com Thu Sep 6 06:31:58 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Sep 6 06:34:36 2007 Subject: [Histonet] Floater sources References: <25355ef80709052257i32794198gd5a64b94758cc1ea@mail.gmail.com> Message-ID: <004201c7f079$8f5aa560$0202a8c0@yourxhtr8hvc4p> Michelle, first. welcome to world of histology. Let's begin at the grossing table- all grossers are trained to wipe off the table after each case- but that doesn't mean floaters can't happen, but the incidence is low third- embedders should have been taught to clean the embedding area after each case and at the end of the day, but this is a good source for contamination. You can see this is the block. fourth- the cutters should wipe off their waterbaths after each block. This probably is the most likely source of contamination. I've inspected some labs where one waterbath was just covered with previous ribbons, attached to the side and floating on the waterbath. fifth- in my experience, it is highly unlikely that floaters came from the stainer just by the shear movement of the slides and water. The bottom line is that everyone needs to be neat and clean and be meticulous. Remember, cleanliness is next to Godliness. I had a testicular seminoma where no matter what we did, floaters still were on cervical bxs and endometrial bxs. The medical and I decided that we would handle cases like these separately. They were processed, embedded, cut and stained separately. Once the case was completed, we changed all the solutions on the tissue processor and stainer. Who ever cut the blocks had to dismantle their knife holder and microtome to clean it thoroughly. A pain in the butt, but was a necessary evil. I hope this helped a little. Good luck. Joe The Toe ----- Original Message ----- From: "Michelle McCoy" To: Sent: Thursday, September 06, 2007 12:57 AM Subject: [Histonet] Floater sources >I was reading some of the old archived messages on floaters, and being > somewhat new to the field of histotechnology was curious about how > floaters > can be attributed to a particular tech who performed work on the block. > For > example, couldn't other sources of contamination be from the automatic > stainer, processor carryover (cassette not completely closed/or if closed, > friable tissue through the slats eg if sponge not used on larger > specimen). > In some previous labs I've worked in- floaters were quickly attributed to > the cutter, embedder or grosser and might result in a "write up". If the > floater is seen in the block how can you differentiate if it came from the > grosser carryover/embedder carryover/processing carryover/unsigned > re-embedder/or even possibly even client carry over between patients or > different specimen types of the same patient. > And if it is not in the block --differentiating between the > cutter/automatic > stainer (I've seen specks of tissue debris in automatic stainers/ sections > falling off the slides and into the reagents etc). Obviously all should be > done to minimize the factors, but I'm just not clear how a single source > is > pinpointed and potentially blamed for the event (depending on the lab > policy). Thanks for any ideas on this. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Thu Sep 6 08:31:31 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Thu Sep 6 07:32:31 2007 Subject: [Histonet] Job Opening (Columbia SC) Message-ID: Professional Pathology Services, PC is one of the regions fastest growing Pathology laboratories located in sunny Columbia South Carolina. Our state of the art histology laboratory has a wall of windows with a scenic view of our own pond. Come join our fun and exciting team of healthcare professionals. We are seeking a full-time certified histology technician ASCP certified w/1-5 years of experience in Histology New Grads welcome to apply This position is for the hours of 9:30PM-6:00AM NO RECRUITERS PLEASE Send r?sum? to Doug@ppspath.com www.ppspath.com Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. From melissa.mazan <@t> tufts.edu Thu Sep 6 08:02:38 2007 From: melissa.mazan <@t> tufts.edu (Melissa Mazan) Date: Thu Sep 6 08:06:41 2007 Subject: [Histonet] Caspase3 In-Reply-To: <200709061148.l86Bmpqk022027@mail-proofpoint-2a.usg.tufts.edu> References: <200709061148.l86Bmpqk022027@mail-proofpoint-2a.usg.tufts.edu> Message-ID: <46DFFA6E.1080702@tufts.edu> Can you elaborate on your high pH EDTA antigen retrieval protocol? Thanks - Melissa Mazan histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Xylene (themagoos) > 2. RE:The great apoptosis stains debate (TUNEL, Caspases, etc) > (Melissa Gonzalez) > 3. Re: RDO decalcifier (Robert Richmond) > 4. Re: IHC anti-cleaved caspase 3 (Carl Hobbs) > 5. avian sperm cell topography (melissah rowe) > 6. Re: IHC anti-cleaved caspase 3 (Thomas Pier) > 7. Re: IHC anti-cleaved caspase 3 (Cheryl Cross) > 8. Fwd: Users of Lab Vision autostainer (Victoria Baker) > 9. Re: Microwaves VENDOR RESPONSE (Phil McArdle) > 10. Re: Xylene (Rene J Buesa) > 11. RE: IHC anti-cleaved caspase 3 (Liz Chlipala) > 12. Freezing mouse testes for frozen sections > (Sarah Clatterbuck Soper) > 13. Olympus BX40 (Mike Pence) > 14. Look for a used stereoscope (Yu, Jian) > 15. RE: RDO decal (Tony Henwood) > 16. PTH antibody reacted with dog tissue... (ChoiUl Soo) > 17. Floater sources (Michelle McCoy) > 18. RE: Xylene (Kemlo Rogerson) > 19. RE: Floater sources (Kemlo Rogerson) > 20. Fwd: Users of Lab Vision autostainer (Victoria Baker) > 21. RE: Xylene (Joseph Kapler) > 22. Unsubscribe (Malam Jacqueline) > 23. Re: Floater sources (Joe Nocito) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 05 Sep 2007 10:21:51 -0700 > From: "themagoos" > Subject: [Histonet] Xylene > To: histonet@lists.utsouthwestern.edu > Message-ID: <46dee5af.14b.450a.1030817715@rushmore.com> > Content-Type: text/plain; charset="iso-8859-1" > > Can somebody tell me if there are any effects on tissue if > there is prolonged processing time in xylene? > > Jason McGough HT(ASCP) > Clinical Laboratory of the Black Hills > Account Representative - Anatomic Pathology > 2805 5th Street > Rapid City, SD 57701 > 605-343-2267 > jmcgough@clinlab.com > > > > > ------------------------------ > > Message: 2 > Date: Wed, 5 Sep 2007 11:30:38 -0700 > From: "Melissa Gonzalez" > Subject: [Histonet] RE:The great apoptosis stains debate (TUNEL, > Caspases, etc) > To: > Message-ID: > <2884B897182A1D438C7BA24B9A8F94A20E701D@hqsvr01mail.cgi.com> > Content-Type: text/plain; charset="iso-8859-1" > > Hi all, > I have been around this mess a few years back, I gave up on TUNEL long ago for the various reasons mentioned on the list recently. > > Per investigators requests, I have tried several ways to demonstrate cell death, however methods such as PI or Annexin staining are more suitable for whole cells, not tissue sections. I have also not had any luck staining for Cytochrome C. > > We routinely use R&D Systems rabbit anti human/mouse active Caspase3 using a high pH (EDTA) based Ag retrieval in a steamer (enzymes do not work). Works like a charm. > > Any other suggestions? I know this topic has been thrown around a lot, but these discussions after all, help us get our jobs done more proficiently. > > Melissa > > > Melissa A. Gonz?lez Edick > R&D, Cell Genesys Inc. > 500 Forbes Blvd > South San Francisco, CA 94080 > p(650) 266-3168 > f (650) 266-3080 > > "It's not enough to believe what you see, you must also understand what you see." -Leonardo Da Vinci > ------------------------------ > > Message: 6 > Date: Tue, 4 Sep 2007 15:18:39 -0400 > From: Cheryl Cross > Subject: Re: [Histonet] TUNEL, Formalin, DNA strand breaks > To: JR R > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <4C1E074D-064A-45D1-BD32-13D7E2222B26@aol.com> > Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed > > Hi all - > > I have been round and round with this issue myself; I was basically > told by people who've attempted it that TUNEL on FFPE sections is not > specific enough due to the formalin issue. > > If you are trying to nail apoptosis, what about anti-active > caspase-3? mind you, that staining can be a bit of a booger too, but > it should be more sensitive and specific for apoptosis (no references > to offer, i'm just repeating what i have been told). > > > Cheryl Cross, DVM, Dipl. ACVP > Researcher > University Corporation for Atmospheric Research > College of Veterinary Medicine > University of Tennessee Department of Pathology > 2407 River Drive, Room A201 > Knoxville, TN 37996-4542 > (423) 967-2724 > fax: 865-974-5616 > ccross@ucar.edu > ------------------------------ > > Message: 5 > Hi Ray, > > Sheesh-- I read (and then lost) the paper maybe 5-7 years ago, back when I was tearing my hair out over high background, or false positives for TUNEL stain in formalin fixed, paraffin embedded arterial sections. I can troubleshoot any immunostain, but the TUNEL and ISEL assays bedeviled me. > > The paper was titled something to the effect of "formaldehyde causes single and double stranded DNA breaks," and was pretty emphatic. I'll look for the article. > > > > I think the assay could work in whole cells for flow, or maybe in frozen tissue. My hunch at this point is that this is one of those rare cases where lots and lots of peer reviewed articles are just plain wrong. > > Hey, if someone has a good protocol, I'd be willing to try it out, and I would be delighted if I turn out to be mistaken. For now, I think TUNEL is the assay of the beast. > > Oh, here is a thought--say you are looking at a 5 micron section through a nucleus. The microtome blade pretty much had to create a lot of double stranded DNA breaks, no? > > > Jerry L. Ricks > Research Scientist > U.W. Medicine at South Lake Union > 815 Mercer Street > Seattle, WA 98109 > (206)-685-7190 > > > From: koellingr@comcast.netTo: rosenfeldtek@hotmail.com; histonet@lists.utsouthwestern.eduSubject: RE: [Histonet] TUNELDate: Fri, 31 Aug 2007 23:17:03 +0000 > > Jerry, > Could you expand on or give references to formalin causing DNA strand breaks? Double strand breaks, single strand, blunt end, overhanging? My pile of papers and having done TUNEL for years says that formalin fixation is a very good technique for TUNEL and many peer-reviewed articles in which TUNEL is used as a technique, use formalin fixation and how can that be if formalin is causing strand breaks? In fact one paper I'm looking at says that extended (5-7 weeks in formalin) fixation causes loss of TUNEL signal. If formalin is causing breaks, you would assume that TUNEL pos signals would increase with extended formalin fixation. Even the use of the monoclonal antibody F7-26, for single stranded DNA, touts formalin fixation for their claims of discriminating apoptosis from necrosis. > > The question asks about extended alcohol fixation but your answer is possibly a lot of false positives because formalin causes DNA breaks. Does this imply that alcohol won't? Have done a lot of TUNEL on alcohol fixed samples. True I couldn't pretreat them and handle them they way I would handle FFPE tissue. Also true that we could argue specificity and ability or not to discriminate apoptosis from necrosis for quite a while. But if formalin itself is causing the breaks in DNA, I and a lot of people are in big trouble with our science projects and experiments. Also I can't envision why 2 cells are showing TUNEL positivity while 2 of the same type of cells right next to them (and getting the same formalin fix), are absolutely clean and there is no background? > > Thanks for any information you can provide. > > Ray Koelling > PhenoPath Laboratories > Seattle, WA > > > > ------------------------------ > > Message: 3 > Date: Wed, 5 Sep 2007 14:56:14 -0400 > From: "Robert Richmond" > Subject: [Histonet] Re: RDO decalcifier > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > I don't see how your hazmats people can tell you what to do to dispose > of RDO, since nobody has any idea what's in it. It's a murky yellow > liquid that continuously throws a black sediment that has to be > filtered out if you're to see your specimen in it. It's sort of the > ultimate secret formula, and I've always wondered why it's so popular > - I've had to use it many different places in my travels. > > I'd suggest either an ordinary proprietary decalcifier that's a clear > liquid, probably hydrochloric acid - or else save money and dilute > your own hydrochloric acid. You can re-use it for a while. When the > time comes to dispose of it, it can be safely diluted with a lot of > water and put down the drain. > > Remember - it's been stressed on this list many times - that "Decal" > is a brand name still in use, and that other brands of decalcifying > solution should not be called Decal. > > Bob Richmond > Samurai Pathologist (never a decalcified Carmelite) > Knoxville TN > > > > ------------------------------ > > Message: 4 > Date: Wed, 5 Sep 2007 20:29:36 +0100 > From: "Carl Hobbs" > Subject: Re: [Histonet] IHC anti-cleaved caspase 3 > To: "Histonet" > Message-ID: <001801c7eff3$18894850$4101a8c0@carlba65530bda> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > Hi. > > Recent Cleaved caspase 3 posts have not mentioned the antibody details: I > would be grateful for the source of these Abs. > Carl > > > > > ------------------------------ > > Message: 5 > Date: Wed, 5 Sep 2007 14:36:50 -0500 > From: melissah rowe > Subject: [Histonet] avian sperm cell topography > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed > > Hi, > > I am a PhD student working on a behavioral ecology project on > Australian fairy-wrens. I am currently trying to find out if I can > determine the dimensions of discrete sections of individual sperm > cells, i.e. acrosome length, nucleus length, midpiece length and tail > length using the material I currently have available. I have prepared > slide smears of sperm that has been previously stained with an eosin- > nigrosin stain (to determine cell viability) and would like to be > able to somehow stain different segments of the sperm so that I can > identify and measure them using an ocular micrometer on a bright > field or phase contrast microscope set up. It is my understanding > that I cannot use fluorescent stains given that I have already used a > colormetric stain (the eosin-nigrosin). If anyone has suggestions for > ways to identify discrete regions of avian (passerine) sperm cells I > would be very grateful to hear from you. > > Many thanks in advance, > melissah > > ----- > melissah rowe > > PhD candidate > Department of Ecology & Evolution > University of Chicago > E. 57th Street, Chicago, IL, 60637 > ph: +1 773-702-3070 > fax: + 1 773-702-9740 > > email: melissah@uchicago.edu > > > > > > ------------------------------ > > Message: 6 > Date: Wed, 05 Sep 2007 14:39:04 -0500 > From: "Thomas Pier" > Subject: Re: [Histonet] IHC anti-cleaved caspase 3 > To: , > Message-ID: <46DEBF88020000DF0000A736@gwmail.medicine.wisc.edu> > Content-Type: text/plain; charset=US-ASCII > > Cell Signalling Technology has a good rabbit monoclonal for Cleaved Caspase-3. > > Tom Pier > > >>>> "Carl Hobbs" 09/05/07 2:29 PM >>> >>>> > Hi. > > Recent Cleaved caspase 3 posts have not mentioned the antibody details: I > would be grateful for the source of these Abs. > Carl > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 7 > Date: Wed, 5 Sep 2007 15:54:35 -0400 > From: Cheryl Cross > Subject: Re: [Histonet] IHC anti-cleaved caspase 3 > To: "Thomas Pier" > Cc: carl.hobbs@kcl.ac.uk, histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed > > I will second the Cell Signaling antibody - we have tried antibodies > from Promega (which gave tons of background staining)...then via this > site i was pointed to Cell Signaling's monoclonal which we tried; the > polyclonal actually has been working very well with minimal > background (we were getting lots of respiratory epithelium and > endothelium lighting up in control animals). I have been using it in > mice with this protocol: > > EDTA with PASCAL > primary antibody 60 minutes, dilution 1:125 > Rabbit envision > DAB plus > > Hope this helps! > > Cheryl Cross, DVM, Dipl. ACVP > Researcher > University Corporation for Atmospheric Research > College of Veterinary Medicine > University of Tennessee Department of Pathology > 2407 River Drive, Room A201 > Knoxville, TN 37996-4542 > (423) 967-2724 > fax: 865-974-5616 > ccross@ucar.edu > > > > > > > > > ------------------------------ > > Message: 8 > Date: Wed, 5 Sep 2007 15:59:45 -0400 > From: "Victoria Baker" > Subject: [Histonet] Fwd: Users of Lab Vision autostainer > To: "Histo Net list server" > Message-ID: > <4f016b690709051259rcdffccet70d7f97ae0457707@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > One more try, first one didn't go through it seems! Thanks > > ---------- Forwarded message ---------- > From: Victoria Baker > Date: Sep 5, 2007 9:02 AM > Subject: Users of Lab Vision autostainer > To: Histo Net list server > > > Hi > > I'm a new user of the Lab Vision autostainer and I'm looking to see if > I can find users in Histo-land that have experience with it. The > facility only works with human tissue and all of the antibodies are > for dx purposes. > > My key questions are as follows: > How many antibodies is your lab running? > How many users do you allow? > How many people do you allow programming rights and at what level of > supervision are they? > > How many of these antibodies are from Lab Vision? > a) are they concentrates or pre-dilutes? > b) for HIER are you using their PT modules/procedures or > your own equipment (microwave, steamer, pressure cooker etc) and in > house designed protocols for retrieval? > c) for digestion do you only use their Pro-K or have you > designed your own in-house methods using other reagents for digestion? > d) do you put your controls on the same slide as the patient? > e) are your controls in-house or commercial? > f) do you have more than one stainer hooked up to one computer system? > > What Version of software do you currently have on your system? > > Any feed back would be very helpful. > > Thanks in advance. > > Vikki Baker > Interim Histology Manager > Mission Hospital System > Asheville, NC > > > > ------------------------------ > > Message: 9 > Date: Wed, 05 Sep 2007 16:00:47 -0400 > From: Phil McArdle > Subject: Re: [Histonet] Microwaves VENDOR RESPONSE > To: Joe Nocito > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <46DF0AEF.7030203@ebsciences.com> > Content-Type: text/plain; charset=UTF-8; format=flowed > > Hi Joe: > > Obviously, a microwave vendor hates to hear microwave horror stories, so > again, no argument - even though I'm not privy to details of the fried > biopsies in question or what type/vintage of microwave, anyone who's > experienced a malfunction involving patient samples doesn't want a > repeat performance. And pathology is, must be, risk averse. > > That said, again, any mechanical or electronic equipment can fail, or > user error can contribute; just look at the "hang-up" problems with > older tissue processors that are now ancient history. It's up to > manufacturers to minimize the possibilities of failure, since patient > care is at stake. Improvement is therefore a continual, ongoing process. > For example, while for years EBS microwave processors incorporated > safety shutdown modes in the event of vent failure, probe failure (open > and closed) and many other component-related issues, about two years ago > we determined that the microwave did not have a comprehensive set of > safeguards to deal with user errors, for example, temperature overshoots > caused by too small a container for a given power setting, or failure to > place the temperature probe in solution. So we developed multiple safety > mechanisms to head off user errors of this sort. > > PMM > From b-frederick <@t> northwestern.edu Thu Sep 6 08:58:01 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Sep 6 08:58:21 2007 Subject: [Histonet] Floater sources In-Reply-To: <25355ef80709052257i32794198gd5a64b94758cc1ea@mail.gmail.com> Message-ID: <000a01c7f08d$f3321870$d00f7ca5@lurie.northwestern.edu> Because histotechs always get the blame!!!!!! Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michelle McCoy Sent: Thursday, September 06, 2007 12:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Floater sources I was reading some of the old archived messages on floaters, and being somewhat new to the field of histotechnology was curious about how floaters can be attributed to a particular tech who performed work on the block. For example, couldn't other sources of contamination be from the automatic stainer, processor carryover (cassette not completely closed/or if closed, friable tissue through the slats eg if sponge not used on larger specimen). In some previous labs I've worked in- floaters were quickly attributed to the cutter, embedder or grosser and might result in a "write up". If the floater is seen in the block how can you differentiate if it came from the grosser carryover/embedder carryover/processing carryover/unsigned re-embedder/or even possibly even client carry over between patients or different specimen types of the same patient. And if it is not in the block --differentiating between the cutter/automatic stainer (I've seen specks of tissue debris in automatic stainers/ sections falling off the slides and into the reagents etc). Obviously all should be done to minimize the factors, but I'm just not clear how a single source is pinpointed and potentially blamed for the event (depending on the lab policy). Thanks for any ideas on this. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alaskagirl1950 <@t> yahoo.com Thu Sep 6 10:02:59 2007 From: alaskagirl1950 <@t> yahoo.com (Patricia Adams) Date: Thu Sep 6 10:03:13 2007 Subject: [Histonet] Floater sources In-Reply-To: <000a01c7f08d$f3321870$d00f7ca5@lurie.northwestern.edu> Message-ID: <404944.82971.qm@web52512.mail.re2.yahoo.com> I agree with that! It seems that sometimes that the need to "write" someone up is the main focus of managers. If they keep that up there will no longer be histotechs to work in hospital settings. None of us wish to make a mistake and cause a patient a wrong diagnosis. But when I started the Pathologist was more willing to work with us and try to find where the "floater" came from. And to fix the problem. Sometimes tissue just blows up on the water bath. And always being in such a hurry to get the slides out earlier and earlier, we might try to work around the junk in the water bath that we can not skim off with a kimwipe. We should just dump the water and again wait for the temp to adjust. But the same people who wish to place blame are also the ones to push us to work faster. Sorry, just a burr under my saddle! Patricia --- Bernice Frederick wrote: > Because histotechs always get the blame!!!!!! > > Bernice Frederick HTL (ASCP) > Northwestern University > Pathology Core Facility > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Michelle > McCoy > Sent: Thursday, September 06, 2007 12:57 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Floater sources > > I was reading some of the old archived messages > on floaters, and being > somewhat new to the field of histotechnology > was curious about how floaters > can be attributed to a particular tech who > performed work on the block. For > example, couldn't other sources of > contamination be from the automatic > stainer, processor carryover (cassette not > completely closed/or if closed, > friable tissue through the slats eg if sponge > not used on larger specimen). > In some previous labs I've worked in- floaters > were quickly attributed to > the cutter, embedder or grosser and might > result in a "write up". If the > floater is seen in the block how can you > differentiate if it came from the > grosser carryover/embedder carryover/processing > carryover/unsigned > re-embedder/or even possibly even client carry > over between patients or > different specimen types of the same patient. > And if it is not in the block --differentiating > between the cutter/automatic > stainer (I've seen specks of tissue debris in > automatic stainers/ sections > falling off the slides and into the reagents > etc). Obviously all should be > done to minimize the factors, but I'm just not > clear how a single source is > pinpointed and potentially blamed for the event > (depending on the lab > policy). Thanks for any ideas on this. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D ____________________________________________________________________________________ Boardwalk for $500? In 2007? Ha! Play Monopoly Here and Now (it's updated for today's economy) at Yahoo! Games. http://get.games.yahoo.com/proddesc?gamekey=monopolyherenow From Doug.Geddes <@t> lhsc.on.ca Thu Sep 6 10:48:08 2007 From: Doug.Geddes <@t> lhsc.on.ca (Doug Geddes) Date: Thu Sep 6 10:48:43 2007 Subject: [Histonet] Microwaves VENDOR RESPONSE In-Reply-To: <003b01c7efd5$52d0da00$d49eae18@yourxhtr8hvc4p> References: <46DD8689.4AA8.00C0.0@ah.org> <46DEB823.4060708@ebsciences.com><003b01c7efd5$52d0da00$d49eae18@yourxhtr8h vc4p> Message-ID: <46DFE8F9.6E1B.0061.0@lhsc.on.ca> Sorry Joe, but budgets aside, I think you get what you pay for. We are a high volume lab and have gone through at least 8 "Wal Mart" microwaves in the last 5 years. We now have the TT Mega, and it is wonderful, it is all about control as much as you can. Morphology is amazing. Doug Geddes BSc, MLT Department of Pathology London Helath Sciences Centre London, ON Canada >>> "Joe Nocito" 2007-09-05 11:56 AM >>> ok, but with the budgets today, many people can't afford a $1749 microwave when they can buy one at Walmart, K-Mart, or somewhere else for $79. Not to make you angry or anything, but I'm wondering how long has it been since you worked in a lab? Histo's budget is the first one cut in the lab because we are not essential. I can't count how many times I fought and fought for my budgets. If I tried to justify a $1749 microwave for special stains, HIER or whatever, I would have been laughed out the manager's office. Just my 4 cents. JTT ----- Original Message ----- From: "Phil McArdle" To: "Kathleen Boozer" Cc: Sent: Wednesday, September 05, 2007 9:07 AM Subject: Re: [Histonet] Microwaves VENDOR RESPONSE > Again, a microwave vendor weighs in (so far I haven't received any > flames), so read at your own risk. :-) > > At the risk of sounding overly and overtly commercial, after reading post > after post of $30,000+ and $18,000 and similarly high figures for lab > microwaves, I really feel the need to set the record straight. Depending > on the usage requirements, we have laboratory microwaves as low as $1749 > for a "bare bones" model for simple operations, to mid-priced units, to > under $11,000 for a vacuum equipped microwave processor capable of the +/- > 0.5 degree C temperature control necessary for tissue processing. > > (I can feel the heat already!) > > There are many compelling reasons to replace a kitchen microwave with a > lab model; feel free to download, read, and even share with colleagues our > Microwave Companion at > > http://www.ebsciences.com/pdf/EBS_MW_COMPANION.pdf > > Best regards, and see you at NSH, > > Phil McArdle > > -- > Phil McArdle > Microwave Product Manager > > Energy Beam Sciences, Inc. > 29-B Kripes Rd. > East Granby, CT 06026 > > Tel: 800.992.9037 x 341 > Mobile: 860.597.6796 > Fax: 860.653.0422 > > pmcardle@ebsciences.com > www.ebsciences.com > > Kathleen Boozer wrote: >> What is the best microwave for a small lab using it only for heating >> Bouin's and Silver Nitrate for special stains? I just can't believe I >> would have to spend $30,000+ or slow down and use a waterbath. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > I skate to where the puck is going to be, not to where it's been. > - Wayne Gretsky > > You must be the change you want to see in the world. > - Mahatma Gandhi > > NOTE: This message, together with any attachments, is intended only for > the use of the individual or entity to which it is addressed and may > contain information that is legally privileged, confidential and exempt > from disclosure. If you are not the intended recipient, however, there's > not a lot I can do about it, and it was probably my mistake anyway. So > please do the right thing and make this e-mail go away. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pathology <@t> inonu.edu.tr Thu Sep 6 10:47:43 2007 From: pathology <@t> inonu.edu.tr (Department of Pathology) Date: Thu Sep 6 10:48:57 2007 Subject: [Histonet] IHC staining weaker in... In-Reply-To: <20070906115124.F2E28EC022@inonu.edu.tr> References: <20070906115124.F2E28EC022@inonu.edu.tr> Message-ID: <20070906154448.M50194@mail.inonu.edu.tr> Dear Participants, our diagnostic pathology lab has a problem. The paraffin sections are stained weakly at the center but better at the periphery of the sections. What could be the reason.??... thanks a lot.. Dr Nasuhi Engin Aydin, malatya Inonu University Hospital, Turkey 44365. ---------- Original Message ----------- From: histonet-request@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Thu, 6 Sep 2007 14:51:24 +0300 (EEST) Subject: Histonet Digest, Vol 46, Issue 6 > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > Today's Topics: > > 1. Xylene (themagoos) > 2. RE:The great apoptosis stains debate (TUNEL, Caspases, etc) > (Melissa Gonzalez) > 3. Re: RDO decalcifier (Robert Richmond) > 4. Re: IHC anti-cleaved caspase 3 (Carl Hobbs) > 5. avian sperm cell topography (melissah rowe) > 6. Re: IHC anti-cleaved caspase 3 (Thomas Pier) > 7. Re: IHC anti-cleaved caspase 3 (Cheryl Cross) > 8. Fwd: Users of Lab Vision autostainer (Victoria Baker) > 9. Re: Microwaves VENDOR RESPONSE (Phil McArdle) > 10. Re: Xylene (Rene J Buesa) > 11. RE: IHC anti-cleaved caspase 3 (Liz Chlipala) > 12. Freezing mouse testes for frozen sections > (Sarah Clatterbuck Soper) > 13. Olympus BX40 (Mike Pence) > 14. Look for a used stereoscope (Yu, Jian) > 15. RE: RDO decal (Tony Henwood) > 16. PTH antibody reacted with dog tissue... (ChoiUl Soo) > 17. Floater sources (Michelle McCoy) > 18. RE: Xylene (Kemlo Rogerson) > 19. RE: Floater sources (Kemlo Rogerson) > 20. Fwd: Users of Lab Vision autostainer (Victoria Baker) > 21. RE: Xylene (Joseph Kapler) > 22. Unsubscribe (Malam Jacqueline) > 23. Re: Floater sources (Joe Nocito) > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 05 Sep 2007 10:21:51 -0700 > From: "themagoos" > Subject: [Histonet] Xylene > To: histonet@lists.utsouthwestern.edu > Message-ID: <46dee5af.14b.450a.1030817715@rushmore.com> > Content-Type: text/plain; charset="iso-8859-1" > > Can somebody tell me if there are any effects on tissue if > there is prolonged processing time in xylene? > > Jason McGough HT(ASCP) > Clinical Laboratory of the Black Hills > Account Representative - Anatomic Pathology > 2805 5th Street > Rapid City, SD 57701 > 605-343-2267 > jmcgough@clinlab.com > > ------------------------------ > > Message: 2 > Date: Wed, 5 Sep 2007 11:30:38 -0700 > From: "Melissa Gonzalez" > Subject: [Histonet] RE:The great apoptosis stains debate (TUNEL, > Caspases, etc) > To: > Message-ID: > <2884B897182A1D438C7BA24B9A8F94A20E701D@hqsvr01mail.cgi.com> > Content-Type: text/plain; charset="iso-8859-1" > > Hi all, > I have been around this mess a few years back, I gave up on TUNEL long ago for > the various reasons mentioned on the list recently. > > Per investigators requests, I have tried several ways to demonstrate cell > death, however methods such as PI or Annexin staining are more suitable for > whole cells, not tissue sections. I have also not had any luck staining for > Cytochrome C. > > We routinely use R&D Systems rabbit anti human/mouse active Caspase3 using a > high pH (EDTA) based Ag retrieval in a steamer (enzymes do not work). Works > like a charm. > > Any other suggestions? I know this topic has been thrown around a lot, but > these discussions after all, help us get our jobs done more proficiently. > > Melissa > > Melissa A. Gonz?lez Edick > R&D, Cell Genesys Inc. > 500 Forbes Blvd > South San Francisco, CA 94080 > p(650) 266-3168 > f (650) 266-3080 > > "It's not enough to believe what you see, you must also understand what you > see." -Leonardo Da Vinci > ------------------------------ > > Message: 6 > Date: Tue, 4 Sep 2007 15:18:39 -0400 > From: Cheryl Cross > Subject: Re: [Histonet] TUNEL, Formalin, DNA strand breaks > To: JR R > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <4C1E074D-064A-45D1-BD32-13D7E2222B26@aol.com> > Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed > > Hi all - > > I have been round and round with this issue myself; I was basically > told by people who've attempted it that TUNEL on FFPE sections is not > specific enough due to the formalin issue. > > If you are trying to nail apoptosis, what about anti-active > caspase-3? mind you, that staining can be a bit of a booger too, but > it should be more sensitive and specific for apoptosis (no references > to offer, i'm just repeating what i have been told). > > Cheryl Cross, DVM, Dipl. ACVP > Researcher > University Corporation for Atmospheric Research > College of Veterinary Medicine > University of Tennessee Department of Pathology > 2407 River Drive, Room A201 > Knoxville, TN 37996-4542 > (423) 967-2724 > fax: 865-974-5616 > ccross@ucar.edu > ------------------------------ > > Message: 5 > Hi Ray, > > Sheesh-- I read (and then lost) the paper maybe 5-7 years ago, back when I was > tearing my hair out over high background, or false positives for TUNEL stain > in formalin fixed, paraffin embedded arterial sections. I can troubleshoot > any immunostain, but the TUNEL and ISEL assays bedeviled me. > > The paper was titled something to the effect of "formaldehyde causes single > and double stranded DNA breaks," and was pretty emphatic. I'll look for the article. > > I think the assay could work in whole cells for flow, or maybe in frozen > tissue. My hunch at this point is that this is one of those rare cases where > lots and lots of peer reviewed articles are just plain wrong. > > Hey, if someone has a good protocol, I'd be willing to try it out, and I would > be delighted if I turn out to be mistaken. For now, I think TUNEL is the > assay of the beast. > > Oh, here is a thought--say you are looking at a 5 micron section through a > nucleus. The microtome blade pretty much had to create a lot of double > stranded DNA breaks, no? > > Jerry L. Ricks > Research Scientist > U.W. Medicine at South Lake Union > 815 Mercer Street > Seattle, WA 98109 > (206)-685-7190 > > From: koellingr@comcast.netTo: rosenfeldtek@hotmail.com; > histonet@lists.utsouthwestern.eduSubject: RE: [Histonet] TUNELDate: Fri, 31 > Aug 2007 23:17:03 +0000 > > Jerry, > Could you expand on or give references to formalin causing DNA strand breaks? > Double strand breaks, single strand, blunt end, overhanging? My pile of > papers and having done TUNEL for years says that formalin fixation is a very > good technique for TUNEL and many peer-reviewed articles in which TUNEL is > used as a technique, use formalin fixation and how can that be if formalin is > causing strand breaks? In fact one paper I'm looking at says that extended (5- > 7 weeks in formalin) fixation causes loss of TUNEL signal. If formalin is > causing breaks, you would assume that TUNEL pos signals would increase with > extended formalin fixation. Even the use of the monoclonal antibody F7-26, > for single stranded DNA, touts formalin fixation for their claims of > discriminating apoptosis from necrosis. > > The question asks about extended alcohol fixation but your answer is possibly > a lot of false positives because formalin causes DNA breaks. Does this imply > that alcohol won't? Have done a lot of TUNEL on alcohol fixed samples. True > I couldn't pretreat them and handle them they way I would handle FFPE tissue. > Also true that we could argue specificity and ability or not to discriminate > apoptosis from necrosis for quite a while. But if formalin itself is causing > the breaks in DNA, I and a lot of people are in big trouble with our science > projects and experiments. Also I can't envision why 2 cells are showing TUNEL > positivity while 2 of the same type of cells right next to them (and getting > the same formalin fix), are absolutely clean and there is no background? > > Thanks for any information you can provide. > > Ray Koelling > PhenoPath Laboratories > Seattle, WA > > ------------------------------ > > Message: 3 > Date: Wed, 5 Sep 2007 14:56:14 -0400 > From: "Robert Richmond" > Subject: [Histonet] Re: RDO decalcifier > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > I don't see how your hazmats people can tell you what to do to dispose > of RDO, since nobody has any idea what's in it. It's a murky yellow > liquid that continuously throws a black sediment that has to be > filtered out if you're to see your specimen in it. It's sort of the > ultimate secret formula, and I've always wondered why it's so popular > - I've had to use it many different places in my travels. > > I'd suggest either an ordinary proprietary decalcifier that's a clear > liquid, probably hydrochloric acid - or else save money and dilute > your own hydrochloric acid. You can re-use it for a while. When the > time comes to dispose of it, it can be safely diluted with a lot of > water and put down the drain. > > Remember - it's been stressed on this list many times - that "Decal" > is a brand name still in use, and that other brands of decalcifying > solution should not be called Decal. > > Bob Richmond > Samurai Pathologist (never a decalcified Carmelite) > Knoxville TN > > ------------------------------ > > Message: 4 > Date: Wed, 5 Sep 2007 20:29:36 +0100 > From: "Carl Hobbs" > Subject: Re: [Histonet] IHC anti-cleaved caspase 3 > To: "Histonet" > Message-ID: <001801c7eff3$18894850$4101a8c0@carlba65530bda> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > Hi. > > Recent Cleaved caspase 3 posts have not mentioned the antibody details: I > would be grateful for the source of these Abs. > Carl > > ------------------------------ > > Message: 5 > Date: Wed, 5 Sep 2007 14:36:50 -0500 > From: melissah rowe > Subject: [Histonet] avian sperm cell topography > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed > > Hi, > > I am a PhD student working on a behavioral ecology project on > Australian fairy-wrens. I am currently trying to find out if I can > determine the dimensions of discrete sections of individual sperm > cells, i.e. acrosome length, nucleus length, midpiece length and tail > length using the material I currently have available. I have prepared > slide smears of sperm that has been previously stained with an eosin- > nigrosin stain (to determine cell viability) and would like to be > able to somehow stain different segments of the sperm so that I can > identify and measure them using an ocular micrometer on a bright > field or phase contrast microscope set up. It is my understanding > that I cannot use fluorescent stains given that I have already used a > colormetric stain (the eosin-nigrosin). If anyone has suggestions for > ways to identify discrete regions of avian (passerine) sperm cells I > would be very grateful to hear from you. > > Many thanks in advance, > melissah > > ----- > melissah rowe > > PhD candidate > Department of Ecology & Evolution > University of Chicago > E. 57th Street, Chicago, IL, 60637 > ph: +1 773-702-3070 > fax: + 1 773-702-9740 > > email: melissah@uchicago.edu > > ------------------------------ > > Message: 6 > Date: Wed, 05 Sep 2007 14:39:04 -0500 > From: "Thomas Pier" > Subject: Re: [Histonet] IHC anti-cleaved caspase 3 > To: , > Message-ID: <46DEBF88020000DF0000A736@gwmail.medicine.wisc.edu> > Content-Type: text/plain; charset=US-ASCII > > Cell Signalling Technology has a good rabbit monoclonal for Cleaved Caspase-3. > > Tom Pier > > >>> "Carl Hobbs" 09/05/07 2:29 PM >>> > Hi. > > Recent Cleaved caspase 3 posts have not mentioned the antibody details: I > would be grateful for the source of these Abs. > Carl > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 7 > Date: Wed, 5 Sep 2007 15:54:35 -0400 > From: Cheryl Cross > Subject: Re: [Histonet] IHC anti-cleaved caspase 3 > To: "Thomas Pier" > Cc: carl.hobbs@kcl.ac.uk, histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed > > I will second the Cell Signaling antibody - we have tried antibodies > from Promega (which gave tons of background staining)...then via this > site i was pointed to Cell Signaling's monoclonal which we tried; the > polyclonal actually has been working very well with minimal > background (we were getting lots of respiratory epithelium and > endothelium lighting up in control animals). I have been using it in > mice with this protocol: > > EDTA with PASCAL > primary antibody 60 minutes, dilution 1:125 > Rabbit envision > DAB plus > > Hope this helps! > > Cheryl Cross, DVM, Dipl. ACVP > Researcher > University Corporation for Atmospheric Research > College of Veterinary Medicine > University of Tennessee Department of Pathology > 2407 River Drive, Room A201 > Knoxville, TN 37996-4542 > (423) 967-2724 > fax: 865-974-5616 > ccross@ucar.edu > > > > > ------------------------------ > > Message: 8 > Date: Wed, 5 Sep 2007 15:59:45 -0400 > From: "Victoria Baker" > Subject: [Histonet] Fwd: Users of Lab Vision autostainer > To: "Histo Net list server" > Message-ID: > <4f016b690709051259rcdffccet70d7f97ae0457707@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > One more try, first one didn't go through it seems! Thanks > > ---------- Forwarded message ---------- > From: Victoria Baker > Date: Sep 5, 2007 9:02 AM > Subject: Users of Lab Vision autostainer > To: Histo Net list server > > Hi > > I'm a new user of the Lab Vision autostainer and I'm looking to see if > I can find users in Histo-land that have experience with it. The > facility only works with human tissue and all of the antibodies are > for dx purposes. > > My key questions are as follows: > How many antibodies is your lab running? > How many users do you allow? > How many people do you allow programming rights and at what level of > supervision are they? > > How many of these antibodies are from Lab Vision? > a) are they concentrates or pre-dilutes? > b) for HIER are you using their PT modules/procedures or > your own equipment (microwave, steamer, pressure cooker etc) and in > house designed protocols for retrieval? > c) for digestion do you only use their Pro-K or have you > designed your own in-house methods using other reagents for digestion? > d) do you put your controls on the same slide as the patient? > e) are your controls in-house or commercial? > f) do you have more than one stainer hooked up to one computer system? > > What Version of software do you currently have on your system? > > Any feed back would be very helpful. > > Thanks in advance. > > Vikki Baker > Interim Histology Manager > Mission Hospital System > Asheville, NC > > ------------------------------ > > Message: 9 > Date: Wed, 05 Sep 2007 16:00:47 -0400 > From: Phil McArdle > Subject: Re: [Histonet] Microwaves VENDOR RESPONSE > To: Joe Nocito > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <46DF0AEF.7030203@ebsciences.com> > Content-Type: text/plain; charset=UTF-8; format=flowed > > Hi Joe: > > Obviously, a microwave vendor hates to hear microwave horror stories, so > again, no argument - even though I'm not privy to details of the fried > biopsies in question or what type/vintage of microwave, anyone who's > experienced a malfunction involving patient samples doesn't want a > repeat performance. And pathology is, must be, risk averse. > > That said, again, any mechanical or electronic equipment can fail, or > user error can contribute; just look at the "hang-up" problems with > older tissue processors that are now ancient history. It's up to > manufacturers to minimize the possibilities of failure, since patient > care is at stake. Improvement is therefore a continual, ongoing process. > For example, while for years EBS microwave processors incorporated > safety shutdown modes in the event of vent failure, probe failure (open > and closed) and many other component-related issues, about two years ago > we determined that the microwave did not have a comprehensive set of > safeguards to deal with user errors, for example, temperature overshoots > caused by too small a container for a given power setting, or failure to > place the temperature probe in solution. So we developed multiple safety > mechanisms to head off user errors of this sort. > > PMM > -- > Phil McArdle > Microwave Product Manager > > Energy Beam Sciences, Inc. > 29-B Kripes Rd. > East Granby, CT 06026 > > Tel: 800.992.9037 x 341 > Mobile: 860.597.6796 > Fax: 860.653.0422 > > pmcardle@ebsciences.com > www.ebsciences.com > > Joe Nocito wrote: > > are you sure it's the willies and not the johnnies? > > Since the magnetron or whatever it was that fried my tissue, I'd wait > > for the traditional processing. Call me a dinosaur, but I really don't > > like doing special stains in the microwave. The only thing I use a > > microwave for at my house is to defrost and reheat stuff (technical > > term). I'm sure there are people out there who can cook a 6 course > > gourmet meal. My best friend can process all types of tissue from > > biopsies to uterus. > > As a matter of fact, he was there grossing when something went wrong > > and told me that he's never seen tissue like that before. > > > > JTT > > ----- Original Message ----- From: "Phil McArdle" > > To: "Joe Nocito" > > Cc: > > Sent: Wednesday, September 05, 2007 11:16 AM > > Subject: Re: [Histonet] Microwaves VENDOR RESPONSE > > > > > >> Hi Joe: > >> > >> No argument there. I'm painfully aware of both a mindset of "a > >> microwave 'should' cost less than $100," and of a dearth of funding > >> for pathology in general (popular shows like CSI to the contrary). :-) > >> I'd still suggest that $1749 is a heck of a lot better (and a lot less > >> laughable) than the $18,000 or $30,000 that's widely quoted and > >> posted, and it's the exact reason we brought an under-$2000 lab > >> microwave to market in the first place. > >> > >> One could argue just as convincingly against all kinds of specialized > >> equipment or reagents on the basis of cost, not just microwaves. We > >> all know of everything from saliva to cheap rice steamers being used > >> in histo labs, and while they may actually be perfectly serviceable, > >> from the standpoint of repeatability or liability, this kind of thing > >> gives me the willies (and that's a technical term). My yardstick is > >> always "what would I be comfortable with if my kid's diagnosis hung in > >> the balance?" > >> > >> Healthy debate is good! > >> > >> Phil > >> -- > >> Phil McArdle > >> Microwave Product Manager > >> > >> Energy Beam Sciences, Inc. > >> 29-B Kripes Rd. > >> East Granby, CT 06026 > >> > >> Tel: 800.992.9037 x 341 > >> Mobile: 860.597.6796 > >> Fax: 860.653.0422 > >> > >> pmcardle@ebsciences.com > >> www.ebsciences.com > >> > >> > >> > >> > >> > >> > >> Joe Nocito wrote: > >>> ok, but with the budgets today, many people can't afford a $1749 > >>> microwave when they can buy one at Walmart, K-Mart, or somewhere else > >>> for $79. > >>> Not to make you angry or anything, but I'm wondering how long has > >>> it been since you worked in a lab? Histo's budget is the first one > >>> cut in the lab because we are not essential. > >>> I can't count how many times I fought and fought for my budgets. > >>> If I tried to justify a $1749 microwave for special stains, HIER > >>> or whatever, I would have been laughed out the manager's office. > >>> Just my 4 cents. > >>> > >>> JTT > >>> ----- Original Message ----- From: "Phil McArdle" > >>> > >>> To: "Kathleen Boozer" > >>> Cc: > >>> Sent: Wednesday, September 05, 2007 9:07 AM > >>> Subject: Re: [Histonet] Microwaves VENDOR RESPONSE > >>> > >>> > >>>> Again, a microwave vendor weighs in (so far I haven't received any > >>>> flames), so read at your own risk. :-) > >>>> > >>>> At the risk of sounding overly and overtly commercial, after reading > >>>> post after post of $30,000+ and $18,000 and similarly high figures > >>>> for lab microwaves, I really feel the need to set the record > >>>> straight. Depending on the usage requirements, we have laboratory > >>>> microwaves as low as $1749 for a "bare bones" model for simple > >>>> operations, to mid-priced units, to under $11,000 for a vacuum > >>>> equipped microwave processor capable of the +/- 0.5 degree C > >>>> temperature control necessary for tissue processing. > >>>> > >>>> (I can feel the heat already!) > >>>> > >>>> There are many compelling reasons to replace a kitchen microwave > >>>> with a lab model; feel free to download, read, and even share with > >>>> colleagues our Microwave Companion at > >>>> > >>>> http://www.ebsciences.com/pdf/EBS_MW_COMPANION.pdf > >>>> > >>>> Best regards, and see you at NSH, > >>>> > >>>> Phil McArdle > >>>> > >>>> -- > >>>> Phil McArdle > >>>> Microwave Product Manager > >>>> > >>>> Energy Beam Sciences, Inc. > >>>> 29-B Kripes Rd. > >>>> East Granby, CT 06026 > >>>> > >>>> Tel: 800.992.9037 x 341 > >>>> Mobile: 860.597.6796 > >>>> Fax: 860.653.0422 > >>>> > >>>> pmcardle@ebsciences.com > >>>> www.ebsciences.com > >>>> > >>>> Kathleen Boozer wrote: > >>>>> What is the best microwave for a small lab using it only for > >>>>> heating Bouin's and Silver Nitrate for special stains? I just > >>>>> can't believe I would have to spend $30,000+ or slow down and use a > >>>>> waterbath. > >>>>> > >>>>> > >>>>> _______________________________________________ > >>>>> Histonet mailing list > >>>>> Histonet@lists.utsouthwestern.edu > >>>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >>>> > >>>> > >>>> > >>>> > >>>> I skate to where the puck is going to be, not to where it's been. > >>>> - Wayne Gretsky > >>>> > >>>> You must be the change you want to see in the world. > >>>> - Mahatma Gandhi > >>>> > >>>> NOTE: This message, together with any attachments, is intended only > >>>> for the use of the individual or entity to which it is addressed and > >>>> may contain information that is legally privileged, confidential and > >>>> exempt from disclosure. If you are not the intended recipient, > >>>> however, there's not a lot I can do about it, and it was probably my > >>>> mistake anyway. So please do the right thing and make this e-mail go > >>>> away. Thank you. > >>>> > >>>> _______________________________________________ > >>>> Histonet mailing list > >>>> Histonet@lists.utsouthwestern.edu > >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >>> > >>> > >>> _______________________________________________ > >>> Histonet mailing list > >>> Histonet@lists.utsouthwestern.edu > >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > >> > >> -- > >> Phil McArdle > >> Microwave Product Manager > >> > >> Energy Beam Sciences, Inc. > >> 29-B Kripes Rd. > >> East Granby, CT 06026 > >> > >> Tel: 800.992.9037 x 341 > >> Mobile: 860.597.6796 > >> Fax: 860.653.0422 > >> > >> pmcardle@ebsciences.com > >> www.ebsciences.com > >> > >> I skate to where the puck is going to be, not to where it's been. > >> - Wayne Gretsky > >> > >> You must be the change you want to see in the world. > >> - Mahatma Gandhi > >> > >> NOTE: This message, together with any attachments, is intended only > >> for the use of the individual or entity to which it is addressed and > >> may contain information that is legally privileged, confidential and > >> exempt from disclosure. If you are not the intended recipient, > >> however, there's not a lot I can do about it, and it was probably my > >> mistake anyway. So please do the right thing and make this e-mail go > >> away. Thank you. > > > > I skate to where the puck is going to be, not to where it's been. > - Wayne Gretsky > > You must be the change you want to see in the world. > - Mahatma Gandhi > > NOTE: This message, together with any attachments, is intended only for > the use of the individual or entity to which it is addressed and may > contain information that is legally privileged, confidential and exempt > from disclosure. If you are not the intended recipient, however, there's > not a lot I can do about it, and it was probably my mistake anyway. So > please do the right thing and make this e-mail go away. Thank you. > > ------------------------------ > > Message: 10 > Date: Wed, 5 Sep 2007 13:01:21 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Xylene > To: themagoos@rushmore.com, histonet@lists.utsouthwestern.edu > Message-ID: <329330.36844.qm@web61219.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Tissues usually become brittle, which difficulties sectioning. > Ren? J. > > themagoos wrote: > Can somebody tell me if there are any effects on tissue if > there is prolonged processing time in xylene? > > Jason McGough HT(ASCP) > Clinical Laboratory of the Black Hills > Account Representative - Anatomic Pathology > 2805 5th Street > Rapid City, SD 57701 > 605-343-2267 > jmcgough@clinlab.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > --------------------------------- > Boardwalk for $500? In 2007? Ha! > Play Monopoly Here and Now (it's updated for today's economy) at Yahoo! Games. > > ------------------------------ > > Message: 11 > Date: Wed, 5 Sep 2007 14:09:02 -0600 > From: "Liz Chlipala" > Subject: RE: [Histonet] IHC anti-cleaved caspase 3 > To: "Cheryl Cross" , "Thomas Pier" > > Cc: carl.hobbs@kcl.ac.uk, histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset="windows-1250" > > I like the cell signaling antibody also, I tried biocare's but did not have > much success with it. The cell signaling antibody also works with pronase > digestion, as well as the EDTA pH9 HIER. We have even used it on bone > sections with the pronase digestion. Works in multiple species, I have used it > on human, rat, mouse, guinea pig, porcine and canine. Our protocol is similar > to Cheryl's. It’s a bit pricy as antibodies go, but I feel its worth it. > Good lot to lot consistency. We have probably gone through about 5 different > lots, with not changing the protocol at all. I have a written protocol if > anyone is interested. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Manager > Premier Laboratory, LLC > P.O. Box 18592 > Boulder, CO 80308 > phone (303) 735-5001 > fax (303) 735-3540 > liz@premierlab.com > www.premierlab.com > > Ship to Address: > > Premier Laboratory, LLC > University of Colorado at Boulder > MCDB, Room A3B40 > Boulder, CO 80309 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Cross Sent: Wednesday, > September 05, 2007 2:01 PM To: Thomas Pier Cc: carl.hobbs@kcl.ac.uk; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] IHC anti-cleaved caspase 3 > > I will second the Cell Signaling antibody - we have tried antibodies from > Promega (which gave tons of background staining)...then via this site i was > pointed to Cell Signaling's monoclonal which we tried; the polyclonal actually > has been working very well with minimal background (we were getting lots of > respiratory epithelium and endothelium lighting up in control animals). I have > been using it in mice with this protocol: > > EDTA with PASCAL > primary antibody 60 minutes, dilution 1:125 Rabbit envision DAB plus > > Hope this helps! > > Cheryl Cross, DVM, Dipl. ACVP > Researcher > University Corporation for Atmospheric Research College of Veterinary Medicine > University of Tennessee Department of Pathology 2407 River Drive, Room A201 > Knoxville, TN 37996-4542 > (423) 967-2724 fax: 865-974-5616 ccross@ucar.edu > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.5.485 / Virus Database: 269.13.5/990 - Release Date: 9/4/2007 10:36 > PM > > No virus found in this outgoing message. > Checked by AVG Free Edition. > Version: 7.5.485 / Virus Database: 269.13.5/990 - Release Date: 9/4/2007 10:36 > PM > > ------------------------------ > > Message: 12 > Date: Wed, 05 Sep 2007 16:13:36 -0400 > From: Sarah Clatterbuck Soper > Subject: [Histonet] Freezing mouse testes for frozen sections > To: histonet@lists.utsouthwestern.edu > Message-ID: <46DF0DF0.2030306@ciwemb.edu> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Hi all, > > I've started attempting to section unfixed frozen mouse testes in order > to placate a specific antibody we have to use. I am new to frozen > sections and I'm having trouble with the testes cracking when I freeze > them. I've tried both freezing in isopentane cooled on liquid nitrogen > and an acetone/dry ice slurry. Either way the testes crack, usually one > big crack end to end. Doesn't seem to be as much of a problem with our > mutant testes, which are about 1/3 the size of wild-type. I wish I > could just trim the wild-type down to a smaller size, but obviously > that's not an option! > > Any recommendations? > > Thanks so much! > > Sarah > > ------------------------------ > > Message: 13 > Date: Wed, 5 Sep 2007 15:49:48 -0500 > From: "Mike Pence" > Subject: [Histonet] Olympus BX40 > To: > Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C701@IS-E2K3.grhs.net> > Content-Type: text/plain; charset="us-ascii" > > Need some help, > > I am looking for a 60x objective for an Olympus microscope BX40. > Would anyone know where I might get a used one or if they even make one > this size for this scope? > > Thanks, > Mike > > ------------------------------ > > Message: 14 > Date: Wed, 5 Sep 2007 16:58:12 -0400 > From: "Yu, Jian" > Subject: [Histonet] Look for a used stereoscope > To: > Message-ID: > <7E0A77BFEB9A1E47A63F978E7116821F07986D6D@1upmc-msx11.acct.upmchs.net> > Content-Type: text/plain; charset="us-ascii" > > Does anyone know a good place to get a used stereoscope? I plan to use > it to examine intestinal tumors in mice. > > Thanks a lot for your information. > > ******************************************************************* > Jian Yu, Ph.D. > University of Pittsburgh Cancer Institute > Hillman Cancer Center Research Pavilion > Office Suite 2.26h > 5117 Centre Avenue, Pittsburgh, PA 15213 > ******************************************************************* > > ------------------------------ > > Message: 15 > Date: Thu, 6 Sep 2007 09:44:45 +1000 > From: "Tony Henwood" > Subject: RE: [Histonet] RDO decal > To: "Rene J Buesa" , "RENEE FISHER" > , > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > There is also the issue of EDTA, which is common in many RDO formulations. > Checking one MSDS for EDTA reveals: > > Ecological Information > Environmental Fate: > When released into the soil, this material is expected to leach into > groundwater. When released into the soil, this material may biodegrade to a > moderate extent. When released into the soil, this material is not expected > to evaporate significantly. When released into water, this material is not > expected to evaporate significantly. This material is not expected to > significantly bioaccumulate. When released into the air, this material is > expected to be readily degraded by photolysis. Environmental Toxicity: > This material is not expected to be toxic to aquatic life. The LC50/96-hour > values for fish are over 100 mg/l. > > So neutralisation may not be required. > If anyone has info to the contrary please advise. > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) > Laboratory Manager & Senior Scientist > The Children's Hospital at Westmead, > Locked Bag 4001, Westmead, 2145, AUSTRALIA. > Tel: 612 9845 3306 > Fax: 612 9845 3318 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, 6 > September 2007 1:07 AM To: RENEE FISHER; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] RDO decal > > Ren?e: > I have not heard of neutralizing RDO, but it would make sense if you want to > be "gentle on your sewer system" BUT prepare to a large emission of carbon > dioxide when attempting to neutralize it with baking soda. RDO + baking soda > (or sodium bicarbonate) will produce water, salt with the acid in RDO > (probably sodium chloride or common salt), and carbon dioxide in > stoichiometrical amounts (1 CO2 per every 1 NaCl). Therefore that > neutralization has to take place in a fumes hood, and you will have to decide > which is worst: delivering acid to the sewer system, or carbon dioxide (the > Greenhouse gas per excellence) to the atmosphere. It will be "your call". > Ren? J. > > RENEE FISHER wrote: > Has anyone heard of neutralizing RDO with baking soda to P.H. 7.0. Our Histo > lab had a hazardous waste assessment, and the consultant suggested we not > throw the RDO down the drain but that we either collect it for waste removal > or neutralize it with baking soda to p.h. 7.0. I have not heard of doing this > and do not know of any procedure for it, everyone, anyone's help will be > greatly appreciated. > > Thanks, > Renee' > > _______________________________________________________________________________________ > > This email may contain confidential protected health information and/or > proprietary information belonging to the sender that is legally privileged > under local, state, or federal law. This information is intended only for the > use of the individual or individuals who have received this. The authorized > recipient of this information is prohibited from disclosing this information > to any other party unless required to do so by law. If you are not the > intended recipient, you are hereby notified that any disclosure, copying, > distribution, or action taken in reliance on the contents of this email is > strictly prohibited. If you have received this email in error, please notify > the sender immediately to arrange for the disposal of this information. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > --------------------------------- > Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: mail, news, > photos & more. _______________________________________________ Histonet > mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************* > This email and any files transmitted with it are confidential and intended > solely for the use of the individual or entity to whom they are addressed. If > you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the > individual sender, and are not necessarily the views of The Children's > Hospital at Westmead > > This note also confirms that this email message has been > virus scanned and although no computer viruses were detected, The Childrens > Hospital at Westmead accepts no liability for any consequential damage > resulting from email containing computer viruses. ********************************************************************** > > ------------------------------ > > Message: 16 > Date: Thu, 6 Sep 2007 11:23:00 +0900 > From: ChoiUl Soo > Subject: [Histonet] PTH antibody reacted with dog tissue... > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="ks_c_5601-1987" > > Hi Histonetters, > > I am interested in anti PTH antibody reacted with dog tissue. > If anyone has successful experience with any PTH antibody with dog tissue, > please tell me one. > > I have searched through the internet, and results came back with abcam and > SantaCruz PTH antibodies predicted to react with them. But they are not sure > on it, just predicted on the basis of sequence homology. > (abcam say 94% homology with human) I don't have good exprience with Santa > Cruz, and never used one by Abcam. Should I rely on it, or find another one? > > Let me hear your experience. > > I would appreciate your advice or comment on this. > > Thank you~. > > Ul Soo Choi, DVM, PhDKRF priority zoonotic disease research institute, College > of Veterinary Medicine, Seoul National University, Shilim9 dong, Gwanakgu, > Seoul, Korea 151-742Tel. 82-02-880-8688 Mobile. 82-016-9228-8634Fax. 82-02-880- > 8662 > > _________________________________________________________________ > ???? ?????? ????, Windows Live Space! > http://www.spaces.live.com > > ------------------------------ > > Message: 17 > Date: Thu, 6 Sep 2007 01:57:04 -0400 > From: "Michelle McCoy" > Subject: [Histonet] Floater sources > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <25355ef80709052257i32794198gd5a64b94758cc1ea@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > I was reading some of the old archived messages on floaters, and being > somewhat new to the field of histotechnology was curious about how floaters > can be attributed to a particular tech who performed work on the block. For > example, couldn't other sources of contamination be from the automatic > stainer, processor carryover (cassette not completely closed/or if closed, > friable tissue through the slats eg if sponge not used on larger specimen). > In some previous labs I've worked in- floaters were quickly attributed to > the cutter, embedder or grosser and might result in a "write up". If the > floater is seen in the block how can you differentiate if it came from the > grosser carryover/embedder carryover/processing carryover/unsigned > re-embedder/or even possibly even client carry over between patients or > different specimen types of the same patient. > And if it is not in the block --differentiating between the cutter/automatic > stainer (I've seen specks of tissue debris in automatic stainers/ sections > falling off the slides and into the reagents etc). Obviously all should be > done to minimize the factors, but I'm just not clear how a single source is > pinpointed and potentially blamed for the event (depending on the lab > policy). Thanks for any ideas on this. > > ------------------------------ > > Message: 18 > Date: Thu, 6 Sep 2007 08:07:38 +0100 > From: "Kemlo Rogerson" > Subject: RE: [Histonet] Xylene > To: , > Message-ID: > <86ADE4EB583CE64799A9924684A0FBBF0222EC6C@wahtntex2.waht.swest.nhs.uk> > Content-Type: text/plain; charset="us-ascii" > > Can somebody tell me if there are any effects on tissue if there is > prolonged processing time in xylene? > > Jason McGough HT(ASCP) > Clinical Laboratory of the Black Hills > Account Representative - Anatomic Pathology > 2805 5th Street > Rapid City, SD 57701 > 605-343-2267 > jmcgough@clinlab.com > > Classically it is said to harden, plus more lipids could be removed. > Personally I'm equivocal about that thought; if properly fixed prior to > processing I would have thought the hardening effects of xylene were > much less than that of the coagulant fixative ethanol. If you do have > hard tissue then there is a restorative fluid one can use which I think > has oil of cedarwood in it but I don't know the formula off hand. I know > it works cos when I was a pup I used it sometimes. > > Kemlo Rogerson > Pathology Manager > DD 01934 647057 or extension 3311 > Mob 07749 754194; Pager 07659 597107; > > Sunshine is delicious, rain is refreshing, wind braces us up, snow is > exhilarating; there is really no such thing as bad weather, only > different kinds of good weather. --John Ruskin > > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. > Please inform me that this message has gone astray before deleting it. > Thank you for your co-operation > > ------------------------------ > > Message: 19 > Date: Thu, 6 Sep 2007 08:17:36 +0100 > From: "Kemlo Rogerson" > Subject: RE: [Histonet] Floater sources > To: "Michelle McCoy" , > > Message-ID: > <86ADE4EB583CE64799A9924684A0FBBF0222EC6D@wahtntex2.waht.swest.nhs.uk> > Content-Type: text/plain; charset="us-ascii" > > I was reading some of the old archived messages on floaters, and being > somewhat new to the field of histotechnology was curious about how > floaters can be attributed to a particular tech who performed work on > the block. For example, couldn't other sources of contamination be from > the automatic stainer, processor carryover (cassette not completely > closed/or if closed, friable tissue through the slats eg if sponge not > used on larger specimen). In some previous labs I've worked in- floaters > were quickly attributed to the cutter, embedder or grosser and might > result in a "write up". If the floater is seen in the block how can you > differentiate if it came from the grosser carryover/embedder > carryover/processing carryover/unsigned re-embedder/or even possibly > even client carry over between patients or different specimen types of > the same patient. And if it is not in the block --differentiating > between the cutter/automatic stainer (I've seen specks of tissue debris > in automatic stainers/ sections falling off the slides and into the > reagents etc). Obviously all should be done to minimize the factors, but > I'm just not clear how a single source is pinpointed and potentially > blamed for the event (depending on the lab policy). Thanks for any ideas > on this. > > You are exactly correct, floaters can be attributed to a variety of > causes, processing machines that aren't regularly changed, transfer on > the cutting up forceps, on the waterbath and on the forceps of the > embedder. If the floater was from a block that was cut, embedded, cut up > before the section with the floater then the culprit is obvious. If the > section was cut by someone who didn't cut the block from which the > floater floated, then the culprit is the embedder, machine,or cutter up. > Realisticaly I would have thought that most floaters would be from the > embedder, cutter up, then sectioner as the latter has the greater > likelihood of seeing the error of his/ her ways. Waxy forceps of messy > forceps, in my experience are the usual culprit but in some instances, > necrotic tumours can shed cells in the processor and even the stainer. > > Floaters are usually obvious as they tend to be at a different level > than the tissue section and I'm afraid they are a fact of life. You can > reduce the incidence but sadly never eradicate them. > > Kemlo Rogerson > Pathology Manager > DD 01934 647057 or extension 3311 > Mob 07749 754194; Pager 07659 597107; > > Sunshine is delicious, rain is refreshing, wind braces us up, snow is > exhilarating; there is really no such thing as bad weather, only > different kinds of good weather. --John Ruskin > > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. > Please inform me that this message has gone astray before deleting it. > Thank you for your co-operation > > ------------------------------ > > Message: 20 > Date: Thu, 6 Sep 2007 04:18:04 -0400 > From: "Victoria Baker" > Subject: [Histonet] Fwd: Users of Lab Vision autostainer > To: histonet > Message-ID: > <4f016b690709060118t532d22c1if250116e0a6c9af1@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > ---------- Forwarded message ---------- > From: Victoria Baker > Date: Sep 5, 2007 9:02 AM > Subject: Users of Lab Vision autostainer > To: Histo Net list server > > Hi > > I'm a new user of the Lab Vision autostainer and I'm looking to see if > I can find users in Histo-land that have experience with it. The > facility only works with human tissue and all of the antibodies are > for dx purposes. > > My key questions are as follows: > How many antibodies is your lab running? > How many users do you allow? > How many people do you allow programming rights and at what level of > supervision are they? > > How many of these antibodies are from Lab Vision? > a) are they concentrates or pre-dilutes? > b) for HIER are you using their PT modules/procedures or > your own equipment (microwave, steamer, pressure cooker etc) and in > house designed protocols for retrieval? > c) for digestion do you only use their Pro-K or have you > designed your own in-house methods using other reagents for digestion? > d) do you put your controls on the same slide as the patient? > e) are your controls in-house or commercial? > f) do you have more than one stainer hooked up to one computer system? > > What Version of software do you currently have on your system? > > Any feed back would be very helpful. > > Thanks in advance. > > Vikki Baker > Interim Histology Manager > Mission Hospital System > Asheville, NC > > ------------------------------ > > Message: 21 > Date: Thu, 6 Sep 2007 02:28:57 -0600 > From: "Joseph Kapler" > Subject: RE: [Histonet] Xylene > To: "Rene J Buesa" , , > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Tissues processed in Xylene (especially over long periods of time) the > tissue becomes very brittle. > > Hope that is the response you were looking for. > > Joseph "DarkWolfe" Kapler > I'm an outsider outside of everything > I'm an outsider outside of everything > I'm an outsider outside of everything > Everything you know. Everything you know > It disturbs me so > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J > Buesa > Sent: Wednesday, September 05, 2007 14:01 > To: themagoos@rushmore.com; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Xylene > > Tissues usually become brittle, which difficulties sectioning. > Ren? J. > > themagoos wrote: > Can somebody tell me if there are any effects on tissue if > there is prolonged processing time in xylene? > > Jason McGough HT(ASCP) > Clinical Laboratory of the Black Hills > Account Representative - Anatomic Pathology > 2805 5th Street > Rapid City, SD 57701 > 605-343-2267 > jmcgough@clinlab.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > --------------------------------- > Boardwalk for $500? In 2007? Ha! > Play Monopoly Here and Now (it's updated for today's economy) at Yahoo! > Games. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.5.485 / Virus Database: 269.13.5/988 - Release Date: 9/4/2007 > 09:14 > > No virus found in this outgoing message. > Checked by AVG Free Edition. > Version: 7.5.485 / Virus Database: 269.13.5/988 - Release Date: 9/4/2007 > 09:14 > > ------------------------------ > > Message: 22 > Date: Thu, 6 Sep 2007 09:59:13 +0100 > From: Malam Jacqueline > Subject: [Histonet] Unsubscribe > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain > > Please would you unsubscribe me as I am retiring tomorrow - thanks > > Jacqui malam > Lancaster > uk > > DISCLAIMER: This e-mail is confidential and privileged. If you are not the > intended recipient please accept our apologies; please do not disclose, copy > or distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. Please > inform postmaster@rli.mbht.nhs.uk that this message has gone astray before > deleting it. Comments or opinions expressed in this email are those of > their respective contributors only. The views expressed do not represent the > views of the Trust, its management or employees. University Hospitals of > Morecambe Bay NHS Trust is not responsible and disclaims any and all > liability for the content of comments written within.Thank you for your > co-operation. > > ------------------------------ > > Message: 23 > Date: Thu, 6 Sep 2007 06:31:58 -0500 > From: "Joe Nocito" > Subject: Re: [Histonet] Floater sources > To: "Michelle McCoy" , > > Message-ID: <004201c7f079$8f5aa560$0202a8c0@yourxhtr8hvc4p> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > Michelle, > first. welcome to world of histology. > Let's begin at the grossing table- all grossers are trained to wipe off the > table after each case- but that doesn't mean floaters can't happen, but the > incidence is low > third- embedders should have been taught to clean the embedding area after > each case and at the end of the day, but this is a good source for > contamination. You can see this is the block. > fourth- the cutters should wipe off their waterbaths after each block. This > probably is the most likely source of contamination. I've inspected some > labs where one waterbath was just covered with previous ribbons, attached to > the side and floating on the waterbath. > fifth- in my experience, it is highly unlikely that floaters came from the > stainer just by the shear movement of the slides and water. > > The bottom line is that everyone needs to be neat and clean and be > meticulous. Remember, cleanliness is next to Godliness. > I had a testicular seminoma where no matter what we did, floaters still were > on cervical bxs and endometrial bxs. The medical and I decided that we > would handle cases like these separately. They were processed, embedded, cut > and stained separately. Once the case was completed, we changed all the > solutions on the tissue processor and stainer. Who ever cut the blocks had > to dismantle their knife holder and microtome to clean it thoroughly. A pain > in the butt, but was a necessary evil. > > I hope this helped a little. Good luck. > > Joe The Toe > ----- Original Message ----- > From: "Michelle McCoy" > To: > Sent: Thursday, September 06, 2007 12:57 AM > Subject: [Histonet] Floater sources > > >I was reading some of the old archived messages on floaters, and being > > somewhat new to the field of histotechnology was curious about how > > floaters > > can be attributed to a particular tech who performed work on the block. > > For > > example, couldn't other sources of contamination be from the automatic > > stainer, processor carryover (cassette not completely closed/or if closed, > > friable tissue through the slats eg if sponge not used on larger > > specimen). > > In some previous labs I've worked in- floaters were quickly attributed to > > the cutter, embedder or grosser and might result in a "write up". If the > > floater is seen in the block how can you differentiate if it came from the > > grosser carryover/embedder carryover/processing carryover/unsigned > > re-embedder/or even possibly even client carry over between patients or > > different specimen types of the same patient. > > And if it is not in the block --differentiating between the > > cutter/automatic > > stainer (I've seen specks of tissue debris in automatic stainers/ sections > > falling off the slides and into the reagents etc). Obviously all should be > > done to minimize the factors, but I'm just not clear how a single source > > is > > pinpointed and potentially blamed for the event (depending on the lab > > policy). Thanks for any ideas on this. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 46, Issue 6 > *************************************** ------- End of Original Message ------- From jqb7 <@t> CDC.GOV Thu Sep 6 10:58:49 2007 From: jqb7 <@t> CDC.GOV (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Thu Sep 6 10:59:12 2007 Subject: [Histonet] Microwaves VENDOR RESPONSE In-Reply-To: <46DFE8F9.6E1B.0061.0@lhsc.on.ca> References: <46DD8689.4AA8.00C0.0@ah.org> <46DEB823.4060708@ebsciences.com><003b01c7efd5$52d0da00$d49eae18@yourxhtr8h vc4p> <46DFE8F9.6E1B.0061.0@lhsc.on.ca> Message-ID: <34BB307EFC9A65429BBB49E330675F7202BDBFEE@LTA3VS003.ees.hhs.gov> Do you use the TT/Mega just for special staining? Jeanine Bartlett Infectious Disease Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Doug Geddes Sent: Thursday, September 06, 2007 11:48 AM To: Kathleen Boozer; Phil McArdle; Joe Nocito Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Microwaves VENDOR RESPONSE Sorry Joe, but budgets aside, I think you get what you pay for. We are a high volume lab and have gone through at least 8 "Wal Mart" microwaves in the last 5 years. We now have the TT Mega, and it is wonderful, it is all about control as much as you can. Morphology is amazing. Doug Geddes BSc, MLT Department of Pathology London Helath Sciences Centre London, ON Canada >>> "Joe Nocito" 2007-09-05 11:56 AM >>> ok, but with the budgets today, many people can't afford a $1749 microwave when they can buy one at Walmart, K-Mart, or somewhere else for $79. Not to make you angry or anything, but I'm wondering how long has it been since you worked in a lab? Histo's budget is the first one cut in the lab because we are not essential. I can't count how many times I fought and fought for my budgets. If I tried to justify a $1749 microwave for special stains, HIER or whatever, I would have been laughed out the manager's office. Just my 4 cents. JTT ----- Original Message ----- From: "Phil McArdle" To: "Kathleen Boozer" Cc: Sent: Wednesday, September 05, 2007 9:07 AM Subject: Re: [Histonet] Microwaves VENDOR RESPONSE > Again, a microwave vendor weighs in (so far I haven't received any > flames), so read at your own risk. :-) > > At the risk of sounding overly and overtly commercial, after reading post > after post of $30,000+ and $18,000 and similarly high figures for lab > microwaves, I really feel the need to set the record straight. Depending > on the usage requirements, we have laboratory microwaves as low as $1749 > for a "bare bones" model for simple operations, to mid-priced units, to > under $11,000 for a vacuum equipped microwave processor capable of the +/- > 0.5 degree C temperature control necessary for tissue processing. > > (I can feel the heat already!) > > There are many compelling reasons to replace a kitchen microwave with a > lab model; feel free to download, read, and even share with colleagues our > Microwave Companion at > > http://www.ebsciences.com/pdf/EBS_MW_COMPANION.pdf > > Best regards, and see you at NSH, > > Phil McArdle > > -- > Phil McArdle > Microwave Product Manager > > Energy Beam Sciences, Inc. > 29-B Kripes Rd. > East Granby, CT 06026 > > Tel: 800.992.9037 x 341 > Mobile: 860.597.6796 > Fax: 860.653.0422 > > pmcardle@ebsciences.com > www.ebsciences.com > > Kathleen Boozer wrote: >> What is the best microwave for a small lab using it only for heating >> Bouin's and Silver Nitrate for special stains? I just can't believe I >> would have to spend $30,000+ or slow down and use a waterbath. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > I skate to where the puck is going to be, not to where it's been. > - Wayne Gretsky > > You must be the change you want to see in the world. > - Mahatma Gandhi > > NOTE: This message, together with any attachments, is intended only for > the use of the individual or entity to which it is addressed and may > contain information that is legally privileged, confidential and exempt > from disclosure. If you are not the intended recipient, however, there's > not a lot I can do about it, and it was probably my mistake anyway. So > please do the right thing and make this e-mail go away. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Sep 6 11:06:23 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 6 11:06:34 2007 Subject: [Histonet] Look for a used stereoscope In-Reply-To: <7E0A77BFEB9A1E47A63F978E7116821F07986D6D@1upmc-msx11.acct.upmchs.net> Message-ID: <411044.56898.qm@web61214.mail.yahoo.com> Have seen them in eBay. Ren? J. "Yu, Jian" wrote: Does anyone know a good place to get a used stereoscope? I plan to use it to examine intestinal tumors in mice. Thanks a lot for your information. ******************************************************************* Jian Yu, Ph.D. University of Pittsburgh Cancer Institute Hillman Cancer Center Research Pavilion Office Suite 2.26h 5117 Centre Avenue, Pittsburgh, PA 15213 ******************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: mail, news, photos & more. From Terry.Marshall <@t> rothgen.nhs.uk Thu Sep 6 11:14:05 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Thu Sep 6 11:14:25 2007 Subject: [Histonet] IHC staining weaker in... Message-ID: <407F05A128805F4C879A33DBA32E618E0189502E@TRFT-EX01.xRothGen.nhs.uk> I thought I had seen every horror possible in a section, but that seems a new one to me! Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Department of Pathology Sent: 06 September 2007 16:48 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC staining weaker in... Dear Participants, our diagnostic pathology lab has a problem. The paraffin sections are stained weakly at the center but better at the periphery of the sections. What could be the reason.??... thanks a lot.. Dr Nasuhi Engin Aydin, malatya Inonu University Hospital, Turkey 44365. ---------- Original Message ----------- From: histonet-request@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Thu, 6 Sep 2007 14:51:24 +0300 (EEST) Subject: Histonet Digest, Vol 46, Issue 6 > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > Today's Topics: > > 1. Xylene (themagoos) > 2. RE:The great apoptosis stains debate (TUNEL, Caspases, etc) > (Melissa Gonzalez) > 3. Re: RDO decalcifier (Robert Richmond) > 4. Re: IHC anti-cleaved caspase 3 (Carl Hobbs) > 5. avian sperm cell topography (melissah rowe) > 6. Re: IHC anti-cleaved caspase 3 (Thomas Pier) > 7. Re: IHC anti-cleaved caspase 3 (Cheryl Cross) > 8. Fwd: Users of Lab Vision autostainer (Victoria Baker) > 9. Re: Microwaves VENDOR RESPONSE (Phil McArdle) > 10. Re: Xylene (Rene J Buesa) > 11. RE: IHC anti-cleaved caspase 3 (Liz Chlipala) > 12. Freezing mouse testes for frozen sections > (Sarah Clatterbuck Soper) > 13. Olympus BX40 (Mike Pence) > 14. Look for a used stereoscope (Yu, Jian) > 15. RE: RDO decal (Tony Henwood) > 16. PTH antibody reacted with dog tissue... (ChoiUl Soo) > 17. Floater sources (Michelle McCoy) > 18. RE: Xylene (Kemlo Rogerson) > 19. RE: Floater sources (Kemlo Rogerson) > 20. Fwd: Users of Lab Vision autostainer (Victoria Baker) > 21. RE: Xylene (Joseph Kapler) > 22. Unsubscribe (Malam Jacqueline) > 23. Re: Floater sources (Joe Nocito) > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 05 Sep 2007 10:21:51 -0700 > From: "themagoos" > Subject: [Histonet] Xylene > To: histonet@lists.utsouthwestern.edu > Message-ID: <46dee5af.14b.450a.1030817715@rushmore.com> > Content-Type: text/plain; charset="iso-8859-1" > > Can somebody tell me if there are any effects on tissue if > there is prolonged processing time in xylene? > > Jason McGough HT(ASCP) > Clinical Laboratory of the Black Hills > Account Representative - Anatomic Pathology > 2805 5th Street > Rapid City, SD 57701 > 605-343-2267 > jmcgough@clinlab.com > > ------------------------------ > > Message: 2 > Date: Wed, 5 Sep 2007 11:30:38 -0700 > From: "Melissa Gonzalez" > Subject: [Histonet] RE:The great apoptosis stains debate (TUNEL, > Caspases, etc) > To: > Message-ID: > <2884B897182A1D438C7BA24B9A8F94A20E701D@hqsvr01mail.cgi.com> > Content-Type: text/plain; charset="iso-8859-1" > > Hi all, > I have been around this mess a few years back, I gave up on TUNEL long ago for > the various reasons mentioned on the list recently. > > Per investigators requests, I have tried several ways to demonstrate cell > death, however methods such as PI or Annexin staining are more suitable for > whole cells, not tissue sections. I have also not had any luck staining for > Cytochrome C. > > We routinely use R&D Systems rabbit anti human/mouse active Caspase3 using a > high pH (EDTA) based Ag retrieval in a steamer (enzymes do not work). Works > like a charm. > > Any other suggestions? I know this topic has been thrown around a lot, but > these discussions after all, help us get our jobs done more proficiently. > > Melissa > > Melissa A. Gonz?lez Edick > R&D, Cell Genesys Inc. > 500 Forbes Blvd > South San Francisco, CA 94080 > p(650) 266-3168 > f (650) 266-3080 > > "It's not enough to believe what you see, you must also understand what you > see." -Leonardo Da Vinci > ------------------------------ > > Message: 6 > Date: Tue, 4 Sep 2007 15:18:39 -0400 > From: Cheryl Cross > Subject: Re: [Histonet] TUNEL, Formalin, DNA strand breaks > To: JR R > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <4C1E074D-064A-45D1-BD32-13D7E2222B26@aol.com> > Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed > > Hi all - > > I have been round and round with this issue myself; I was basically > told by people who've attempted it that TUNEL on FFPE sections is not > specific enough due to the formalin issue. > > If you are trying to nail apoptosis, what about anti-active > caspase-3? mind you, that staining can be a bit of a booger too, but > it should be more sensitive and specific for apoptosis (no references > to offer, i'm just repeating what i have been told). > > Cheryl Cross, DVM, Dipl. ACVP > Researcher > University Corporation for Atmospheric Research > College of Veterinary Medicine > University of Tennessee Department of Pathology > 2407 River Drive, Room A201 > Knoxville, TN 37996-4542 > (423) 967-2724 > fax: 865-974-5616 > ccross@ucar.edu > ------------------------------ > > Message: 5 > Hi Ray, > > Sheesh-- I read (and then lost) the paper maybe 5-7 years ago, back when I was > tearing my hair out over high background, or false positives for TUNEL stain > in formalin fixed, paraffin embedded arterial sections. I can troubleshoot > any immunostain, but the TUNEL and ISEL assays bedeviled me. > > The paper was titled something to the effect of "formaldehyde causes single > and double stranded DNA breaks," and was pretty emphatic. I'll look for the article. > > I think the assay could work in whole cells for flow, or maybe in frozen > tissue. My hunch at this point is that this is one of those rare cases where > lots and lots of peer reviewed articles are just plain wrong. > > Hey, if someone has a good protocol, I'd be willing to try it out, and I would > be delighted if I turn out to be mistaken. For now, I think TUNEL is the > assay of the beast. > > Oh, here is a thought--say you are looking at a 5 micron section through a > nucleus. The microtome blade pretty much had to create a lot of double > stranded DNA breaks, no? > > Jerry L. Ricks > Research Scientist > U.W. Medicine at South Lake Union > 815 Mercer Street > Seattle, WA 98109 > (206)-685-7190 > > From: koellingr@comcast.netTo: rosenfeldtek@hotmail.com; > histonet@lists.utsouthwestern.eduSubject: RE: [Histonet] TUNELDate: Fri, 31 > Aug 2007 23:17:03 +0000 > > Jerry, > Could you expand on or give references to formalin causing DNA strand breaks? > Double strand breaks, single strand, blunt end, overhanging? My pile of > papers and having done TUNEL for years says that formalin fixation is a very > good technique for TUNEL and many peer-reviewed articles in which TUNEL is > used as a technique, use formalin fixation and how can that be if formalin is > causing strand breaks? In fact one paper I'm looking at says that extended (5- > 7 weeks in formalin) fixation causes loss of TUNEL signal. If formalin is > causing breaks, you would assume that TUNEL pos signals would increase with > extended formalin fixation. Even the use of the monoclonal antibody F7-26, > for single stranded DNA, touts formalin fixation for their claims of > discriminating apoptosis from necrosis. > > The question asks about extended alcohol fixation but your answer is possibly > a lot of false positives because formalin causes DNA breaks. Does this imply > that alcohol won't? Have done a lot of TUNEL on alcohol fixed samples. True > I couldn't pretreat them and handle them they way I would handle FFPE tissue. > Also true that we could argue specificity and ability or not to discriminate > apoptosis from necrosis for quite a while. But if formalin itself is causing > the breaks in DNA, I and a lot of people are in big trouble with our science > projects and experiments. Also I can't envision why 2 cells are showing TUNEL > positivity while 2 of the same type of cells right next to them (and getting > the same formalin fix), are absolutely clean and there is no background? > > Thanks for any information you can provide. > > Ray Koelling > PhenoPath Laboratories > Seattle, WA > > ------------------------------ > > Message: 3 > Date: Wed, 5 Sep 2007 14:56:14 -0400 > From: "Robert Richmond" > Subject: [Histonet] Re: RDO decalcifier > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > I don't see how your hazmats people can tell you what to do to dispose > of RDO, since nobody has any idea what's in it. It's a murky yellow > liquid that continuously throws a black sediment that has to be > filtered out if you're to see your specimen in it. It's sort of the > ultimate secret formula, and I've always wondered why it's so popular > - I've had to use it many different places in my travels. > > I'd suggest either an ordinary proprietary decalcifier that's a clear > liquid, probably hydrochloric acid - or else save money and dilute > your own hydrochloric acid. You can re-use it for a while. When the > time comes to dispose of it, it can be safely diluted with a lot of > water and put down the drain. > > Remember - it's been stressed on this list many times - that "Decal" > is a brand name still in use, and that other brands of decalcifying > solution should not be called Decal. > > Bob Richmond > Samurai Pathologist (never a decalcified Carmelite) > Knoxville TN > > ------------------------------ > > Message: 4 > Date: Wed, 5 Sep 2007 20:29:36 +0100 > From: "Carl Hobbs" > Subject: Re: [Histonet] IHC anti-cleaved caspase 3 > To: "Histonet" > Message-ID: <001801c7eff3$18894850$4101a8c0@carlba65530bda> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > Hi. > > Recent Cleaved caspase 3 posts have not mentioned the antibody details: I > would be grateful for the source of these Abs. > Carl > > ------------------------------ > > Message: 5 > Date: Wed, 5 Sep 2007 14:36:50 -0500 > From: melissah rowe > Subject: [Histonet] avian sperm cell topography > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed > > Hi, > > I am a PhD student working on a behavioral ecology project on > Australian fairy-wrens. I am currently trying to find out if I can > determine the dimensions of discrete sections of individual sperm > cells, i.e. acrosome length, nucleus length, midpiece length and tail > length using the material I currently have available. I have prepared > slide smears of sperm that has been previously stained with an eosin- > nigrosin stain (to determine cell viability) and would like to be > able to somehow stain different segments of the sperm so that I can > identify and measure them using an ocular micrometer on a bright > field or phase contrast microscope set up. It is my understanding > that I cannot use fluorescent stains given that I have already used a > colormetric stain (the eosin-nigrosin). If anyone has suggestions for > ways to identify discrete regions of avian (passerine) sperm cells I > would be very grateful to hear from you. > > Many thanks in advance, > melissah > > ----- > melissah rowe > > PhD candidate > Department of Ecology & Evolution > University of Chicago > E. 57th Street, Chicago, IL, 60637 > ph: +1 773-702-3070 > fax: + 1 773-702-9740 > > email: melissah@uchicago.edu > > ------------------------------ > > Message: 6 > Date: Wed, 05 Sep 2007 14:39:04 -0500 > From: "Thomas Pier" > Subject: Re: [Histonet] IHC anti-cleaved caspase 3 > To: , > Message-ID: <46DEBF88020000DF0000A736@gwmail.medicine.wisc.edu> > Content-Type: text/plain; charset=US-ASCII > > Cell Signalling Technology has a good rabbit monoclonal for Cleaved Caspase-3. > > Tom Pier > > >>> "Carl Hobbs" 09/05/07 2:29 PM >>> > Hi. > > Recent Cleaved caspase 3 posts have not mentioned the antibody details: I > would be grateful for the source of these Abs. > Carl > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 7 > Date: Wed, 5 Sep 2007 15:54:35 -0400 > From: Cheryl Cross > Subject: Re: [Histonet] IHC anti-cleaved caspase 3 > To: "Thomas Pier" > Cc: carl.hobbs@kcl.ac.uk, histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed > > I will second the Cell Signaling antibody - we have tried antibodies > from Promega (which gave tons of background staining)...then via this > site i was pointed to Cell Signaling's monoclonal which we tried; the > polyclonal actually has been working very well with minimal > background (we were getting lots of respiratory epithelium and > endothelium lighting up in control animals). I have been using it in > mice with this protocol: > > EDTA with PASCAL > primary antibody 60 minutes, dilution 1:125 > Rabbit envision > DAB plus > > Hope this helps! > > Cheryl Cross, DVM, Dipl. ACVP > Researcher > University Corporation for Atmospheric Research > College of Veterinary Medicine > University of Tennessee Department of Pathology > 2407 River Drive, Room A201 > Knoxville, TN 37996-4542 > (423) 967-2724 > fax: 865-974-5616 > ccross@ucar.edu > > > > > ------------------------------ > > Message: 8 > Date: Wed, 5 Sep 2007 15:59:45 -0400 > From: "Victoria Baker" > Subject: [Histonet] Fwd: Users of Lab Vision autostainer > To: "Histo Net list server" > Message-ID: > <4f016b690709051259rcdffccet70d7f97ae0457707@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > One more try, first one didn't go through it seems! Thanks > > ---------- Forwarded message ---------- > From: Victoria Baker > Date: Sep 5, 2007 9:02 AM > Subject: Users of Lab Vision autostainer > To: Histo Net list server > > Hi > > I'm a new user of the Lab Vision autostainer and I'm looking to see if > I can find users in Histo-land that have experience with it. The > facility only works with human tissue and all of the antibodies are > for dx purposes. > > My key questions are as follows: > How many antibodies is your lab running? > How many users do you allow? > How many people do you allow programming rights and at what level of > supervision are they? > > How many of these antibodies are from Lab Vision? > a) are they concentrates or pre-dilutes? > b) for HIER are you using their PT modules/procedures or > your own equipment (microwave, steamer, pressure cooker etc) and in > house designed protocols for retrieval? > c) for digestion do you only use their Pro-K or have you > designed your own in-house methods using other reagents for digestion? > d) do you put your controls on the same slide as the patient? > e) are your controls in-house or commercial? > f) do you have more than one stainer hooked up to one computer system? > > What Version of software do you currently have on your system? > > Any feed back would be very helpful. > > Thanks in advance. > > Vikki Baker > Interim Histology Manager > Mission Hospital System > Asheville, NC > > ------------------------------ > > Message: 9 > Date: Wed, 05 Sep 2007 16:00:47 -0400 > From: Phil McArdle > Subject: Re: [Histonet] Microwaves VENDOR RESPONSE > To: Joe Nocito > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <46DF0AEF.7030203@ebsciences.com> > Content-Type: text/plain; charset=UTF-8; format=flowed > > Hi Joe: > > Obviously, a microwave vendor hates to hear microwave horror stories, so > again, no argument - even though I'm not privy to details of the fried > biopsies in question or what type/vintage of microwave, anyone who's > experienced a malfunction involving patient samples doesn't want a > repeat performance. And pathology is, must be, risk averse. > > That said, again, any mechanical or electronic equipment can fail, or > user error can contribute; just look at the "hang-up" problems with > older tissue processors that are now ancient history. It's up to > manufacturers to minimize the possibilities of failure, since patient > care is at stake. Improvement is therefore a continual, ongoing process. > For example, while for years EBS microwave processors incorporated > safety shutdown modes in the event of vent failure, probe failure (open > and closed) and many other component-related issues, about two years ago > we determined that the microwave did not have a comprehensive set of > safeguards to deal with user errors, for example, temperature overshoots > caused by too small a container for a given power setting, or failure to > place the temperature probe in solution. So we developed multiple safety > mechanisms to head off user errors of this sort. > > PMM > -- > Phil McArdle > Microwave Product Manager > > Energy Beam Sciences, Inc. > 29-B Kripes Rd. > East Granby, CT 06026 > > Tel: 800.992.9037 x 341 > Mobile: 860.597.6796 > Fax: 860.653.0422 > > pmcardle@ebsciences.com > www.ebsciences.com > > Joe Nocito wrote: > > are you sure it's the willies and not the johnnies? > > Since the magnetron or whatever it was that fried my tissue, I'd wait > > for the traditional processing. Call me a dinosaur, but I really don't > > like doing special stains in the microwave. The only thing I use a > > microwave for at my house is to defrost and reheat stuff (technical > > term). I'm sure there are people out there who can cook a 6 course > > gourmet meal. My best friend can process all types of tissue from > > biopsies to uterus. > > As a matter of fact, he was there grossing when something went wrong > > and told me that he's never seen tissue like that before. > > > > JTT > > ----- Original Message ----- From: "Phil McArdle" > > To: "Joe Nocito" > > Cc: > > Sent: Wednesday, September 05, 2007 11:16 AM > > Subject: Re: [Histonet] Microwaves VENDOR RESPONSE > > > > > >> Hi Joe: > >> > >> No argument there. I'm painfully aware of both a mindset of "a > >> microwave 'should' cost less than $100," and of a dearth of funding > >> for pathology in general (popular shows like CSI to the contrary). :-) > >> I'd still suggest that $1749 is a heck of a lot better (and a lot less > >> laughable) than the $18,000 or $30,000 that's widely quoted and > >> posted, and it's the exact reason we brought an under-$2000 lab > >> microwave to market in the first place. > >> > >> One could argue just as convincingly against all kinds of specialized > >> equipment or reagents on the basis of cost, not just microwaves. We > >> all know of everything from saliva to cheap rice steamers being used > >> in histo labs, and while they may actually be perfectly serviceable, > >> from the standpoint of repeatability or liability, this kind of thing > >> gives me the willies (and that's a technical term). My yardstick is > >> always "what would I be comfortable with if my kid's diagnosis hung in > >> the balance?" > >> > >> Healthy debate is good! > >> > >> Phil > >> -- > >> Phil McArdle > >> Microwave Product Manager > >> > >> Energy Beam Sciences, Inc. > >> 29-B Kripes Rd. > >> East Granby, CT 06026 > >> > >> Tel: 800.992.9037 x 341 > >> Mobile: 860.597.6796 > >> Fax: 860.653.0422 > >> > >> pmcardle@ebsciences.com > >> www.ebsciences.com > >> > >> > >> > >> > >> > >> > >> Joe Nocito wrote: > >>> ok, but with the budgets today, many people can't afford a $1749 > >>> microwave when they can buy one at Walmart, K-Mart, or somewhere else > >>> for $79. > >>> Not to make you angry or anything, but I'm wondering how long has > >>> it been since you worked in a lab? Histo's budget is the first one > >>> cut in the lab because we are not essential. > >>> I can't count how many times I fought and fought for my budgets. > >>> If I tried to justify a $1749 microwave for special stains, HIER > >>> or whatever, I would have been laughed out the manager's office. > >>> Just my 4 cents. > >>> > >>> JTT > >>> ----- Original Message ----- From: "Phil McArdle" > >>> > >>> To: "Kathleen Boozer" > >>> Cc: > >>> Sent: Wednesday, September 05, 2007 9:07 AM > >>> Subject: Re: [Histonet] Microwaves VENDOR RESPONSE > >>> > >>> > >>>> Again, a microwave vendor weighs in (so far I haven't received any > >>>> flames), so read at your own risk. :-) > >>>> > >>>> At the risk of sounding overly and overtly commercial, after reading > >>>> post after post of $30,000+ and $18,000 and similarly high figures > >>>> for lab microwaves, I really feel the need to set the record > >>>> straight. Depending on the usage requirements, we have laboratory > >>>> microwaves as low as $1749 for a "bare bones" model for simple > >>>> operations, to mid-priced units, to under $11,000 for a vacuum > >>>> equipped microwave processor capable of the +/- 0.5 degree C > >>>> temperature control necessary for tissue processing. > >>>> > >>>> (I can feel the heat already!) > >>>> > >>>> There are many compelling reasons to replace a kitchen microwave > >>>> with a lab model; feel free to download, read, and even share with > >>>> colleagues our Microwave Companion at > >>>> > >>>> http://www.ebsciences.com/pdf/EBS_MW_COMPANION.pdf > >>>> > >>>> Best regards, and see you at NSH, > >>>> > >>>> Phil McArdle > >>>> > >>>> -- > >>>> Phil McArdle > >>>> Microwave Product Manager > >>>> > >>>> Energy Beam Sciences, Inc. > >>>> 29-B Kripes Rd. > >>>> East Granby, CT 06026 > >>>> > >>>> Tel: 800.992.9037 x 341 > >>>> Mobile: 860.597.6796 > >>>> Fax: 860.653.0422 > >>>> > >>>> pmcardle@ebsciences.com > >>>> www.ebsciences.com > >>>> > >>>> Kathleen Boozer wrote: > >>>>> What is the best microwave for a small lab using it only for > >>>>> heating Bouin's and Silver Nitrate for special stains? I just > >>>>> can't believe I would have to spend $30,000+ or slow down and use a > >>>>> waterbath. > >>>>> > >>>>> > >>>>> _______________________________________________ > >>>>> Histonet mailing list > >>>>> Histonet@lists.utsouthwestern.edu > >>>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >>>> > >>>> > >>>> > >>>> > >>>> I skate to where the puck is going to be, not to where it's been. > >>>> - Wayne Gretsky > >>>> > >>>> You must be the change you want to see in the world. > >>>> - Mahatma Gandhi > >>>> > >>>> NOTE: This message, together with any attachments, is intended only > >>>> for the use of the individual or entity to which it is addressed and > >>>> may contain information that is legally privileged, confidential and > >>>> exempt from disclosure. If you are not the intended recipient, > >>>> however, there's not a lot I can do about it, and it was probably my > >>>> mistake anyway. So please do the right thing and make this e-mail go > >>>> away. Thank you. > >>>> > >>>> _______________________________________________ > >>>> Histonet mailing list > >>>> Histonet@lists.utsouthwestern.edu > >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >>> > >>> > >>> _______________________________________________ > >>> Histonet mailing list > >>> Histonet@lists.utsouthwestern.edu > >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > >> > >> -- > >> Phil McArdle > >> Microwave Product Manager > >> > >> Energy Beam Sciences, Inc. > >> 29-B Kripes Rd. > >> East Granby, CT 06026 > >> > >> Tel: 800.992.9037 x 341 > >> Mobile: 860.597.6796 > >> Fax: 860.653.0422 > >> > >> pmcardle@ebsciences.com > >> www.ebsciences.com > >> > >> I skate to where the puck is going to be, not to where it's been. > >> - Wayne Gretsky > >> > >> You must be the change you want to see in the world. > >> - Mahatma Gandhi > >> > >> NOTE: This message, together with any attachments, is intended only > >> for the use of the individual or entity to which it is addressed and > >> may contain information that is legally privileged, confidential and > >> exempt from disclosure. If you are not the intended recipient, > >> however, there's not a lot I can do about it, and it was probably my > >> mistake anyway. So please do the right thing and make this e-mail go > >> away. Thank you. > > > > I skate to where the puck is going to be, not to where it's been. > - Wayne Gretsky > > You must be the change you want to see in the world. > - Mahatma Gandhi > > NOTE: This message, together with any attachments, is intended only for > the use of the individual or entity to which it is addressed and may > contain information that is legally privileged, confidential and exempt > from disclosure. If you are not the intended recipient, however, there's > not a lot I can do about it, and it was probably my mistake anyway. So > please do the right thing and make this e-mail go away. Thank you. > > ------------------------------ > > Message: 10 > Date: Wed, 5 Sep 2007 13:01:21 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Xylene > To: themagoos@rushmore.com, histonet@lists.utsouthwestern.edu > Message-ID: <329330.36844.qm@web61219.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Tissues usually become brittle, which difficulties sectioning. > Ren? J. > > themagoos wrote: > Can somebody tell me if there are any effects on tissue if > there is prolonged processing time in xylene? > > Jason McGough HT(ASCP) > Clinical Laboratory of the Black Hills > Account Representative - Anatomic Pathology > 2805 5th Street > Rapid City, SD 57701 > 605-343-2267 > jmcgough@clinlab.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > --------------------------------- > Boardwalk for $500? In 2007? Ha! > Play Monopoly Here and Now (it's updated for today's economy) at Yahoo! Games. > > ------------------------------ > > Message: 11 > Date: Wed, 5 Sep 2007 14:09:02 -0600 > From: "Liz Chlipala" > Subject: RE: [Histonet] IHC anti-cleaved caspase 3 > To: "Cheryl Cross" , "Thomas Pier" > > Cc: carl.hobbs@kcl.ac.uk, histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset="windows-1250" > > I like the cell signaling antibody also, I tried biocare's but did not have > much success with it. The cell signaling antibody also works with pronase > digestion, as well as the EDTA pH9 HIER. We have even used it on bone > sections with the pronase digestion. Works in multiple species, I have used it > on human, rat, mouse, guinea pig, porcine and canine. Our protocol is similar > to Cheryl's. It’s a bit pricy as antibodies go, but I feel its worth it. > Good lot to lot consistency. We have probably gone through about 5 different > lots, with not changing the protocol at all. I have a written protocol if > anyone is interested. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Manager > Premier Laboratory, LLC > P.O. Box 18592 > Boulder, CO 80308 > phone (303) 735-5001 > fax (303) 735-3540 > liz@premierlab.com > www.premierlab.com > > Ship to Address: > > Premier Laboratory, LLC > University of Colorado at Boulder > MCDB, Room A3B40 > Boulder, CO 80309 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Cross Sent: Wednesday, > September 05, 2007 2:01 PM To: Thomas Pier Cc: carl.hobbs@kcl.ac.uk; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] IHC anti-cleaved caspase 3 > > I will second the Cell Signaling antibody - we have tried antibodies from > Promega (which gave tons of background staining)...then via this site i was > pointed to Cell Signaling's monoclonal which we tried; the polyclonal actually > has been working very well with minimal background (we were getting lots of > respiratory epithelium and endothelium lighting up in control animals). I have > been using it in mice with this protocol: > > EDTA with PASCAL > primary antibody 60 minutes, dilution 1:125 Rabbit envision DAB plus > > Hope this helps! > > Cheryl Cross, DVM, Dipl. ACVP > Researcher > University Corporation for Atmospheric Research College of Veterinary Medicine > University of Tennessee Department of Pathology 2407 River Drive, Room A201 > Knoxville, TN 37996-4542 > (423) 967-2724 fax: 865-974-5616 ccross@ucar.edu > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.5.485 / Virus Database: 269.13.5/990 - Release Date: 9/4/2007 10:36 > PM > > No virus found in this outgoing message. > Checked by AVG Free Edition. > Version: 7.5.485 / Virus Database: 269.13.5/990 - Release Date: 9/4/2007 10:36 > PM > > ------------------------------ > > Message: 12 > Date: Wed, 05 Sep 2007 16:13:36 -0400 > From: Sarah Clatterbuck Soper > Subject: [Histonet] Freezing mouse testes for frozen sections > To: histonet@lists.utsouthwestern.edu > Message-ID: <46DF0DF0.2030306@ciwemb.edu> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Hi all, > > I've started attempting to section unfixed frozen mouse testes in order > to placate a specific antibody we have to use. I am new to frozen > sections and I'm having trouble with the testes cracking when I freeze > them. I've tried both freezing in isopentane cooled on liquid nitrogen > and an acetone/dry ice slurry. Either way the testes crack, usually one > big crack end to end. Doesn't seem to be as much of a problem with our > mutant testes, which are about 1/3 the size of wild-type. I wish I > could just trim the wild-type down to a smaller size, but obviously > that's not an option! > > Any recommendations? > > Thanks so much! > > Sarah > > ------------------------------ > > Message: 13 > Date: Wed, 5 Sep 2007 15:49:48 -0500 > From: "Mike Pence" > Subject: [Histonet] Olympus BX40 > To: > Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C701@IS-E2K3.grhs.net> > Content-Type: text/plain; charset="us-ascii" > > Need some help, > > I am looking for a 60x objective for an Olympus microscope BX40. > Would anyone know where I might get a used one or if they even make one > this size for this scope? > > Thanks, > Mike > > ------------------------------ > > Message: 14 > Date: Wed, 5 Sep 2007 16:58:12 -0400 > From: "Yu, Jian" > Subject: [Histonet] Look for a used stereoscope > To: > Message-ID: > <7E0A77BFEB9A1E47A63F978E7116821F07986D6D@1upmc-msx11.acct.upmchs.net> > Content-Type: text/plain; charset="us-ascii" > > Does anyone know a good place to get a used stereoscope? I plan to use > it to examine intestinal tumors in mice. > > Thanks a lot for your information. > > ******************************************************************* > Jian Yu, Ph.D. > University of Pittsburgh Cancer Institute > Hillman Cancer Center Research Pavilion > Office Suite 2.26h > 5117 Centre Avenue, Pittsburgh, PA 15213 > ******************************************************************* > > ------------------------------ > > Message: 15 > Date: Thu, 6 Sep 2007 09:44:45 +1000 > From: "Tony Henwood" > Subject: RE: [Histonet] RDO decal > To: "Rene J Buesa" , "RENEE FISHER" > , > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > There is also the issue of EDTA, which is common in many RDO formulations. > Checking one MSDS for EDTA reveals: > > Ecological Information > Environmental Fate: > When released into the soil, this material is expected to leach into > groundwater. When released into the soil, this material may biodegrade to a > moderate extent. When released into the soil, this material is not expected > to evaporate significantly. When released into water, this material is not > expected to evaporate significantly. This material is not expected to > significantly bioaccumulate. When released into the air, this material is > expected to be readily degraded by photolysis. Environmental Toxicity: > This material is not expected to be toxic to aquatic life. The LC50/96-hour > values for fish are over 100 mg/l. > > So neutralisation may not be required. > If anyone has info to the contrary please advise. > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) > Laboratory Manager & Senior Scientist > The Children's Hospital at Westmead, > Locked Bag 4001, Westmead, 2145, AUSTRALIA. > Tel: 612 9845 3306 > Fax: 612 9845 3318 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, 6 > September 2007 1:07 AM To: RENEE FISHER; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] RDO decal > > Ren?e: > I have not heard of neutralizing RDO, but it would make sense if you want to > be "gentle on your sewer system" BUT prepare to a large emission of carbon > dioxide when attempting to neutralize it with baking soda. RDO + baking soda > (or sodium bicarbonate) will produce water, salt with the acid in RDO > (probably sodium chloride or common salt), and carbon dioxide in > stoichiometrical amounts (1 CO2 per every 1 NaCl). Therefore that > neutralization has to take place in a fumes hood, and you will have to decide > which is worst: delivering acid to the sewer system, or carbon dioxide (the > Greenhouse gas per excellence) to the atmosphere. It will be "your call". > Ren? J. > > RENEE FISHER wrote: > Has anyone heard of neutralizing RDO with baking soda to P.H. 7.0. Our Histo > lab had a hazardous waste assessment, and the consultant suggested we not > throw the RDO down the drain but that we either collect it for waste removal > or neutralize it with baking soda to p.h. 7.0. I have not heard of doing this > and do not know of any procedure for it, everyone, anyone's help will be > greatly appreciated. > > Thanks, > Renee' > > _______________________________________________________________________________________ > > This email may contain confidential protected health information and/or > proprietary information belonging to the sender that is legally privileged > under local, state, or federal law. This information is intended only for the > use of the individual or individuals who have received this. The authorized > recipient of this information is prohibited from disclosing this information > to any other party unless required to do so by law. If you are not the > intended recipient, you are hereby notified that any disclosure, copying, > distribution, or action taken in reliance on the contents of this email is > strictly prohibited. If you have received this email in error, please notify > the sender immediately to arrange for the disposal of this information. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > --------------------------------- > Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: mail, news, > photos & more. _______________________________________________ Histonet > mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************* > This email and any files transmitted with it are confidential and intended > solely for the use of the individual or entity to whom they are addressed. If > you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the > individual sender, and are not necessarily the views of The Children's > Hospital at Westmead > > This note also confirms that this email message has been > virus scanned and although no computer viruses were detected, The Childrens > Hospital at Westmead accepts no liability for any consequential damage > resulting from email containing computer viruses. ********************************************************************** > > ------------------------------ > > Message: 16 > Date: Thu, 6 Sep 2007 11:23:00 +0900 > From: ChoiUl Soo > Subject: [Histonet] PTH antibody reacted with dog tissue... > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="ks_c_5601-1987" > > Hi Histonetters, > > I am interested in anti PTH antibody reacted with dog tissue. > If anyone has successful experience with any PTH antibody with dog tissue, > please tell me one. > > I have searched through the internet, and results came back with abcam and > SantaCruz PTH antibodies predicted to react with them. But they are not sure > on it, just predicted on the basis of sequence homology. > (abcam say 94% homology with human) I don't have good exprience with Santa > Cruz, and never used one by Abcam. Should I rely on it, or find another one? > > Let me hear your experience. > > I would appreciate your advice or comment on this. > > Thank you~. > > Ul Soo Choi, DVM, PhDKRF priority zoonotic disease research institute, College > of Veterinary Medicine, Seoul National University, Shilim9 dong, Gwanakgu, > Seoul, Korea 151-742Tel. 82-02-880-8688 Mobile. 82-016-9228-8634Fax. 82-02-880- > 8662 > > _________________________________________________________________ > ???? ?????? ????, Windows Live Space! > http://www.spaces.live.com > > ------------------------------ > > Message: 17 > Date: Thu, 6 Sep 2007 01:57:04 -0400 > From: "Michelle McCoy" > Subject: [Histonet] Floater sources > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <25355ef80709052257i32794198gd5a64b94758cc1ea@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > I was reading some of the old archived messages on floaters, and being > somewhat new to the field of histotechnology was curious about how floaters > can be attributed to a particular tech who performed work on the block. For > example, couldn't other sources of contamination be from the automatic > stainer, processor carryover (cassette not completely closed/or if closed, > friable tissue through the slats eg if sponge not used on larger specimen). > In some previous labs I've worked in- floaters were quickly attributed to > the cutter, embedder or grosser and might result in a "write up". If the > floater is seen in the block how can you differentiate if it came from the > grosser carryover/embedder carryover/processing carryover/unsigned > re-embedder/or even possibly even client carry over between patients or > different specimen types of the same patient. > And if it is not in the block --differentiating between the cutter/automatic > stainer (I've seen specks of tissue debris in automatic stainers/ sections > falling off the slides and into the reagents etc). Obviously all should be > done to minimize the factors, but I'm just not clear how a single source is > pinpointed and potentially blamed for the event (depending on the lab > policy). Thanks for any ideas on this. > > ------------------------------ > > Message: 18 > Date: Thu, 6 Sep 2007 08:07:38 +0100 > From: "Kemlo Rogerson" > Subject: RE: [Histonet] Xylene > To: , > Message-ID: > <86ADE4EB583CE64799A9924684A0FBBF0222EC6C@wahtntex2.waht.swest.nhs.uk> > Content-Type: text/plain; charset="us-ascii" > > Can somebody tell me if there are any effects on tissue if there is > prolonged processing time in xylene? > > Jason McGough HT(ASCP) > Clinical Laboratory of the Black Hills > Account Representative - Anatomic Pathology > 2805 5th Street > Rapid City, SD 57701 > 605-343-2267 > jmcgough@clinlab.com > > Classically it is said to harden, plus more lipids could be removed. > Personally I'm equivocal about that thought; if properly fixed prior to > processing I would have thought the hardening effects of xylene were > much less than that of the coagulant fixative ethanol. If you do have > hard tissue then there is a restorative fluid one can use which I think > has oil of cedarwood in it but I don't know the formula off hand. I know > it works cos when I was a pup I used it sometimes. > > Kemlo Rogerson > Pathology Manager > DD 01934 647057 or extension 3311 > Mob 07749 754194; Pager 07659 597107; > > Sunshine is delicious, rain is refreshing, wind braces us up, snow is > exhilarating; there is really no such thing as bad weather, only > different kinds of good weather. --John Ruskin > > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. > Please inform me that this message has gone astray before deleting it. > Thank you for your co-operation > > ------------------------------ > > Message: 19 > Date: Thu, 6 Sep 2007 08:17:36 +0100 > From: "Kemlo Rogerson" > Subject: RE: [Histonet] Floater sources > To: "Michelle McCoy" , > > Message-ID: > <86ADE4EB583CE64799A9924684A0FBBF0222EC6D@wahtntex2.waht.swest.nhs.uk> > Content-Type: text/plain; charset="us-ascii" > > I was reading some of the old archived messages on floaters, and being > somewhat new to the field of histotechnology was curious about how > floaters can be attributed to a particular tech who performed work on > the block. For example, couldn't other sources of contamination be from > the automatic stainer, processor carryover (cassette not completely > closed/or if closed, friable tissue through the slats eg if sponge not > used on larger specimen). In some previous labs I've worked in- floaters > were quickly attributed to the cutter, embedder or grosser and might > result in a "write up". If the floater is seen in the block how can you > differentiate if it came from the grosser carryover/embedder > carryover/processing carryover/unsigned re-embedder/or even possibly > even client carry over between patients or different specimen types of > the same patient. And if it is not in the block --differentiating > between the cutter/automatic stainer (I've seen specks of tissue debris > in automatic stainers/ sections falling off the slides and into the > reagents etc). Obviously all should be done to minimize the factors, but > I'm just not clear how a single source is pinpointed and potentially > blamed for the event (depending on the lab policy). Thanks for any ideas > on this. > > You are exactly correct, floaters can be attributed to a variety of > causes, processing machines that aren't regularly changed, transfer on > the cutting up forceps, on the waterbath and on the forceps of the > embedder. If the floater was from a block that was cut, embedded, cut up > before the section with the floater then the culprit is obvious. If the > section was cut by someone who didn't cut the block from which the > floater floated, then the culprit is the embedder, machine,or cutter up. > Realisticaly I would have thought that most floaters would be from the > embedder, cutter up, then sectioner as the latter has the greater > likelihood of seeing the error of his/ her ways. Waxy forceps of messy > forceps, in my experience are the usual culprit but in some instances, > necrotic tumours can shed cells in the processor and even the stainer. > > Floaters are usually obvious as they tend to be at a different level > than the tissue section and I'm afraid they are a fact of life. You can > reduce the incidence but sadly never eradicate them. > > Kemlo Rogerson > Pathology Manager > DD 01934 647057 or extension 3311 > Mob 07749 754194; Pager 07659 597107; > > Sunshine is delicious, rain is refreshing, wind braces us up, snow is > exhilarating; there is really no such thing as bad weather, only > different kinds of good weather. --John Ruskin > > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. > Please inform me that this message has gone astray before deleting it. > Thank you for your co-operation > > ------------------------------ > > Message: 20 > Date: Thu, 6 Sep 2007 04:18:04 -0400 > From: "Victoria Baker" > Subject: [Histonet] Fwd: Users of Lab Vision autostainer > To: histonet > Message-ID: > <4f016b690709060118t532d22c1if250116e0a6c9af1@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > ---------- Forwarded message ---------- > From: Victoria Baker > Date: Sep 5, 2007 9:02 AM > Subject: Users of Lab Vision autostainer > To: Histo Net list server > > Hi > > I'm a new user of the Lab Vision autostainer and I'm looking to see if > I can find users in Histo-land that have experience with it. The > facility only works with human tissue and all of the antibodies are > for dx purposes. > > My key questions are as follows: > How many antibodies is your lab running? > How many users do you allow? > How many people do you allow programming rights and at what level of > supervision are they? > > How many of these antibodies are from Lab Vision? > a) are they concentrates or pre-dilutes? > b) for HIER are you using their PT modules/procedures or > your own equipment (microwave, steamer, pressure cooker etc) and in > house designed protocols for retrieval? > c) for digestion do you only use their Pro-K or have you > designed your own in-house methods using other reagents for digestion? > d) do you put your controls on the same slide as the patient? > e) are your controls in-house or commercial? > f) do you have more than one stainer hooked up to one computer system? > > What Version of software do you currently have on your system? > > Any feed back would be very helpful. > > Thanks in advance. > > Vikki Baker > Interim Histology Manager > Mission Hospital System > Asheville, NC > > ------------------------------ > > Message: 21 > Date: Thu, 6 Sep 2007 02:28:57 -0600 > From: "Joseph Kapler" > Subject: RE: [Histonet] Xylene > To: "Rene J Buesa" , , > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Tissues processed in Xylene (especially over long periods of time) the > tissue becomes very brittle. > > Hope that is the response you were looking for. > > Joseph "DarkWolfe" Kapler > I'm an outsider outside of everything > I'm an outsider outside of everything > I'm an outsider outside of everything > Everything you know. Everything you know > It disturbs me so > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J > Buesa > Sent: Wednesday, September 05, 2007 14:01 > To: themagoos@rushmore.com; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Xylene > > Tissues usually become brittle, which difficulties sectioning. > Ren? J. > > themagoos wrote: > Can somebody tell me if there are any effects on tissue if > there is prolonged processing time in xylene? > > Jason McGough HT(ASCP) > Clinical Laboratory of the Black Hills > Account Representative - Anatomic Pathology > 2805 5th Street > Rapid City, SD 57701 > 605-343-2267 > jmcgough@clinlab.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > --------------------------------- > Boardwalk for $500? In 2007? Ha! > Play Monopoly Here and Now (it's updated for today's economy) at Yahoo! > Games. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.5.485 / Virus Database: 269.13.5/988 - Release Date: 9/4/2007 > 09:14 > > No virus found in this outgoing message. > Checked by AVG Free Edition. > Version: 7.5.485 / Virus Database: 269.13.5/988 - Release Date: 9/4/2007 > 09:14 > > ------------------------------ > > Message: 22 > Date: Thu, 6 Sep 2007 09:59:13 +0100 > From: Malam Jacqueline > Subject: [Histonet] Unsubscribe > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain > > Please would you unsubscribe me as I am retiring tomorrow - thanks > > Jacqui malam > Lancaster > uk > > DISCLAIMER: This e-mail is confidential and privileged. If you are not the > intended recipient please accept our apologies; please do not disclose, copy > or distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. Please > inform postmaster@rli.mbht.nhs.uk that this message has gone astray before > deleting it. Comments or opinions expressed in this email are those of > their respective contributors only. The views expressed do not represent the > views of the Trust, its management or employees. University Hospitals of > Morecambe Bay NHS Trust is not responsible and disclaims any and all > liability for the content of comments written within.Thank you for your > co-operation. > > ------------------------------ > > Message: 23 > Date: Thu, 6 Sep 2007 06:31:58 -0500 > From: "Joe Nocito" > Subject: Re: [Histonet] Floater sources > To: "Michelle McCoy" , > > Message-ID: <004201c7f079$8f5aa560$0202a8c0@yourxhtr8hvc4p> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > Michelle, > first. welcome to world of histology. > Let's begin at the grossing table- all grossers are trained to wipe off the > table after each case- but that doesn't mean floaters can't happen, but the > incidence is low > third- embedders should have been taught to clean the embedding area after > each case and at the end of the day, but this is a good source for > contamination. You can see this is the block. > fourth- the cutters should wipe off their waterbaths after each block. This > probably is the most likely source of contamination. I've inspected some > labs where one waterbath was just covered with previous ribbons, attached to > the side and floating on the waterbath. > fifth- in my experience, it is highly unlikely that floaters came from the > stainer just by the shear movement of the slides and water. > > The bottom line is that everyone needs to be neat and clean and be > meticulous. Remember, cleanliness is next to Godliness. > I had a testicular seminoma where no matter what we did, floaters still were > on cervical bxs and endometrial bxs. The medical and I decided that we > would handle cases like these separately. They were processed, embedded, cut > and stained separately. Once the case was completed, we changed all the > solutions on the tissue processor and stainer. Who ever cut the blocks had > to dismantle their knife holder and microtome to clean it thoroughly. A pain > in the butt, but was a necessary evil. > > I hope this helped a little. Good luck. > > Joe The Toe > ----- Original Message ----- > From: "Michelle McCoy" > To: > Sent: Thursday, September 06, 2007 12:57 AM > Subject: [Histonet] Floater sources > > >I was reading some of the old archived messages on floaters, and being > > somewhat new to the field of histotechnology was curious about how > > floaters > > can be attributed to a particular tech who performed work on the block. > > For > > example, couldn't other sources of contamination be from the automatic > > stainer, processor carryover (cassette not completely closed/or if closed, > > friable tissue through the slats eg if sponge not used on larger > > specimen). > > In some previous labs I've worked in- floaters were quickly attributed to > > the cutter, embedder or grosser and might result in a "write up". If the > > floater is seen in the block how can you differentiate if it came from the > > grosser carryover/embedder carryover/processing carryover/unsigned > > re-embedder/or even possibly even client carry over between patients or > > different specimen types of the same patient. > > And if it is not in the block --differentiating between the > > cutter/automatic > > stainer (I've seen specks of tissue debris in automatic stainers/ sections > > falling off the slides and into the reagents etc). Obviously all should be > > done to minimize the factors, but I'm just not clear how a single source > > is > > pinpointed and potentially blamed for the event (depending on the lab > > policy). Thanks for any ideas on this. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 46, Issue 6 > *************************************** ------- End of Original Message ------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Thu Sep 6 11:31:09 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Thu Sep 6 11:31:22 2007 Subject: [Histonet] MMP9 Message-ID: <006501c7f0a3$552a00a0$6401a8c0@Patsy> Does anyone have experience with MMP9 ms monoclonal antibody from Abcam on ffpe tissue they could possibly share with me. This one IHC is driving me crazy. Thank you, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC 12635 Montview Blvd. Ste.215 Aurora, Colorado 80045 Phone: 720-859-4060 Fax: 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org From PMonfils <@t> Lifespan.org Thu Sep 6 11:34:05 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu Sep 6 11:34:19 2007 Subject: [Histonet] IHC staining weaker in... In-Reply-To: <20070906154448.M50194@mail.inonu.edu.tr> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273CD9@LSRIEXCH1.lsmaster.lifespan.org> What is your standard fixative? Have you made any changes in routine fixation recently? This sounds like a fixation problem. As a core research facility, we receive all kinds of tissues from various researchers, often in a variety of fixatives. I have noticed the artifact you describe in some tissues fixed in certain slow-penetrating fixatives. Some aggressive fixatives give very nice preservation in small tissue samples, but when larger tissues are immersed in such fixatives, the rapid fixation of the outer layer of the tissue actually sets up a barrier to penetration of the fixative into the deeper levels. The result is a poorly fixed interior with a very well fixed exterior, and the poorly fixed deeper areas often stain poorly. From katherine-walters <@t> uiowa.edu Thu Sep 6 11:36:26 2007 From: katherine-walters <@t> uiowa.edu (Walters, Katherine S) Date: Thu Sep 6 11:36:39 2007 Subject: [Histonet] damaged cartilage Message-ID: Does anyone know of a good antibody for damaged cartilage? I found one very expensive one in Canada, but it is cost prohibitive. Thanks, Kathy Walters Katherine Walters Histology Director Central Microscopy Research Facility University of Iowa 85 Eckstein Medical Research Building Iowa City, Iowa 52242-1101 phone: (319) 335-8142 fax: (319) 384-4469 katherine-walters@uiowa.edu From PMonfils <@t> Lifespan.org Thu Sep 6 11:38:01 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu Sep 6 11:38:12 2007 Subject: [Histonet] IHC staining weaker in - P.S. In-Reply-To: <20070906154448.M50194@mail.inonu.edu.tr> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273CDA@LSRIEXCH1.lsmaster.lifespan.org> P.S. Of course, even with a faster penetrating fixative like formalin, the same artifact can be produced simply by insufficient time in the fixative to allow full penetration. Are you seeing this artifact mostly in larger tissue sections? From lblazek <@t> digestivespecialists.com Thu Sep 6 11:56:17 2007 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Sep 6 11:55:00 2007 Subject: [Histonet] Job Opening (Columbia SC) In-Reply-To: References: Message-ID: <1F937FB30BDB7C4A9F39F83FEA8D379F3E4C09@bruexchange1.digestivespecialists.com> Is the pond lighted? Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Thursday, September 06, 2007 9:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Job Opening (Columbia SC) Professional Pathology Services, PC is one of the regions fastest growing Pathology laboratories located in sunny Columbia South Carolina. Our state of the art histology laboratory has a wall of windows with a scenic view of our own pond. Come join our fun and exciting team of healthcare professionals. We are seeking a full-time certified histology technician ASCP certified w/1-5 years of experience in Histology New Grads welcome to apply This position is for the hours of 9:30PM-6:00AM NO RECRUITERS PLEASE Send r?sum? to Doug@ppspath.com www.ppspath.com Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Thu Sep 6 12:07:01 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Sep 6 12:07:22 2007 Subject: [Histonet] damaged cartilage In-Reply-To: <2178E0E6E9924927BF700A83FCF25DFF@PremierLab.local> References: <2178E0E6E9924927BF700A83FCF25DFF@PremierLab.local> Message-ID: What type of tissue are you looking at? Why don=92t you just stain with = toluidine blue or safranin O. Both of these stain proteoglycan, areas = of cartilage damage will show loss of staining. There are also pathology = parameters such as fibrillation, loss of chondrocytes and chondrocyte = cloning that are characteristic of cartilage damage. If you feel you = need to use a IHC stain, you could try collagen II. Articular cartilage = should stain positive for collagen II. But I would initally try the = special stains. Liz=20 Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com =20 Ship to Address: =20 Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu = [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Walters, = Katherine S Sent: Thursday, September 06, 2007 10:46 AM To: histonet@pathology.swmed.edu Subject: [Histonet] damaged cartilage Does anyone know of a good antibody for damaged cartilage? I found one = very expensive one in Canada, but it is cost prohibitive. =20 Thanks, =20 Kathy Walters=20 =20 Katherine Walters Histology Director Central Microscopy Research Facility University of Iowa 85 Eckstein Medical Research Building Iowa City, Iowa 52242-1101 phone: (319) 335-8142 fax: (319) 384-4469 katherine-walters@uiowa.edu=20 =20 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG Free Edition.=20 Version: 7.5.485 / Virus Database: 269.13.6/991 - Release Date: 9/5/2007 = 2:55 PM =20 No virus found in this outgoing message. Checked by AVG Free Edition.=20 Version: 7.5.485 / Virus Database: 269.13.6/991 - Release Date: 9/5/2007 = 2:55 PM =20 From doug <@t> ppspath.com Thu Sep 6 13:08:14 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Thu Sep 6 12:08:51 2007 Subject: [Histonet] Job Opening (Columbia SC) In-Reply-To: <1F937FB30BDB7C4A9F39F83FEA8D379F3E4C09@bruexchange1.digestivespecialists.com> Message-ID: Of course :) Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Thursday, September 06, 2007 11:56 AM To: Douglas D Deltour; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Job Opening (Columbia SC) Is the pond lighted? Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Thursday, September 06, 2007 9:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Job Opening (Columbia SC) Professional Pathology Services, PC is one of the regions fastest growing Pathology laboratories located in sunny Columbia South Carolina. Our state of the art histology laboratory has a wall of windows with a scenic view of our own pond. Come join our fun and exciting team of healthcare professionals. We are seeking a full-time certified histology technician ASCP certified w/1-5 years of experience in Histology New Grads welcome to apply This position is for the hours of 9:30PM-6:00AM NO RECRUITERS PLEASE Send r?sum? to Doug@ppspath.com www.ppspath.com Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alaskagirl1950 <@t> yahoo.com Thu Sep 6 12:10:37 2007 From: alaskagirl1950 <@t> yahoo.com (Patricia Adams) Date: Thu Sep 6 12:10:50 2007 Subject: [Histonet] Job Opening (Columbia SC) Message-ID: <559818.86032.qm@web52505.mail.re2.yahoo.com> Swans? --- Douglas D Deltour wrote: > Of course :) > > Douglas D. Deltour HT(ASCP) > Histology Manager > Professional Pathology Services, PC > One Science Court > Suite 200 > Columbia, SC 29203 > Office (803)252-1913 > Fax (803)254-3262 > Doug@ppspath.com > ***************************************************** > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of > the individual or entity to > which it is addressed and may contain > information that is privileged, > confidential and exempt from disclosure under > applicable law. If the reader > of this message is not the intended recipient, > you are hereby notified that > any dissemination, distribution, or copying of > this communication is > strictly prohibited by law. If you have > received this communication in > error, please notify me immediately. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Blazek, > Linda > Sent: Thursday, September 06, 2007 11:56 AM > To: Douglas D Deltour; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Job Opening (Columbia > SC) > > Is the pond lighted? > > Linda Blazek HT (ASCP) > Manager/Supervisor > GI Pathology of Dayton > 7415 Brandt Pike > Huber Heights, OH 45424 > Phone: (937) 293-4424 ext 7118 > Email: lblazek@digestivespecialists.com > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Douglas D > Deltour > Sent: Thursday, September 06, 2007 9:32 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Job Opening (Columbia SC) > > Professional Pathology Services, PC is one of > the regions fastest growing > Pathology laboratories located in sunny > Columbia South Carolina. Our state > of the art histology laboratory has a wall of > windows with a scenic view of > our own pond. Come join our fun and exciting > team of healthcare > professionals. > > > > We are seeking a full-time certified histology > technician > > ASCP certified w/1-5 years of experience in > Histology > > New Grads welcome to apply > This position is for the hours of 9:30PM-6:00AM > > NO RECRUITERS PLEASE > > Send r?sum? to Doug@ppspath.com > www.ppspath.com > > > > > > Douglas D. Deltour HT(ASCP) > > Histology Manager > > Professional Pathology Services, PC > > One Science Court > > Suite 200 > > Columbia, SC 29203 > > Office (803)252-1913 > > Fax (803)254-3262 > > Doug@ppspath.com > > ***************************************************** > > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of > the individual or entity to > which it is addressed and may contain > information that is privileged, > confidential and exempt from disclosure under > applicable law. If the reader > of this message is not the intended recipient, > you are hereby notified that > any dissemination, distribution, or copying of > this communication is > strictly prohibited by law. If you have > received this communication in > error, please notify me immediately. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D ____________________________________________________________________________________ Luggage? GPS? Comic books? Check out fitting gifts for grads at Yahoo! Search http://search.yahoo.com/search?fr=oni_on_mail&p=graduation+gifts&cs=bz From doug <@t> ppspath.com Thu Sep 6 13:14:43 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Thu Sep 6 12:15:20 2007 Subject: [Histonet] Job Opening (Columbia SC) In-Reply-To: <1F937FB30BDB7C4A9F39F83FEA8D379F3E4C09@bruexchange1.digestivespecialists.com> Message-ID: For all of you that found the "pond" amusing here you go. :) http://maps.google.com/?ie=UTF8&ll=34.099354,-80.964872&spn=0.00239,0.003648 &t=k&z=18&om=1 Bring your fishing pole! Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Thursday, September 06, 2007 11:56 AM To: Douglas D Deltour; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Job Opening (Columbia SC) Is the pond lighted? Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Thursday, September 06, 2007 9:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Job Opening (Columbia SC) Professional Pathology Services, PC is one of the regions fastest growing Pathology laboratories located in sunny Columbia South Carolina. Our state of the art histology laboratory has a wall of windows with a scenic view of our own pond. Come join our fun and exciting team of healthcare professionals. We are seeking a full-time certified histology technician ASCP certified w/1-5 years of experience in Histology New Grads welcome to apply This position is for the hours of 9:30PM-6:00AM NO RECRUITERS PLEASE Send r?sum? to Doug@ppspath.com www.ppspath.com Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Thu Sep 6 13:18:59 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Thu Sep 6 12:19:35 2007 Subject: {SPAM?} RE: [Histonet] Job Opening (Columbia SC) In-Reply-To: <559818.86032.qm@web52505.mail.re2.yahoo.com> Message-ID: Ducks and turtles! Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patricia Adams Sent: Thursday, September 06, 2007 12:11 PM To: Douglas D Deltour; HistoNet Subject: {SPAM?} RE: [Histonet] Job Opening (Columbia SC) Swans? --- Douglas D Deltour wrote: > Of course :) > > Douglas D. Deltour HT(ASCP) > Histology Manager > Professional Pathology Services, PC > One Science Court > Suite 200 > Columbia, SC 29203 > Office (803)252-1913 > Fax (803)254-3262 > Doug@ppspath.com > ***************************************************** > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of > the individual or entity to > which it is addressed and may contain > information that is privileged, > confidential and exempt from disclosure under > applicable law. If the reader > of this message is not the intended recipient, > you are hereby notified that > any dissemination, distribution, or copying of > this communication is > strictly prohibited by law. If you have > received this communication in > error, please notify me immediately. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Blazek, > Linda > Sent: Thursday, September 06, 2007 11:56 AM > To: Douglas D Deltour; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Job Opening (Columbia > SC) > > Is the pond lighted? > > Linda Blazek HT (ASCP) > Manager/Supervisor > GI Pathology of Dayton > 7415 Brandt Pike > Huber Heights, OH 45424 > Phone: (937) 293-4424 ext 7118 > Email: lblazek@digestivespecialists.com > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Douglas D > Deltour > Sent: Thursday, September 06, 2007 9:32 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Job Opening (Columbia SC) > > Professional Pathology Services, PC is one of > the regions fastest growing > Pathology laboratories located in sunny > Columbia South Carolina. Our state > of the art histology laboratory has a wall of > windows with a scenic view of > our own pond. Come join our fun and exciting > team of healthcare > professionals. > > > > We are seeking a full-time certified histology > technician > > ASCP certified w/1-5 years of experience in > Histology > > New Grads welcome to apply > This position is for the hours of 9:30PM-6:00AM > > NO RECRUITERS PLEASE > > Send r?sum? to Doug@ppspath.com > www.ppspath.com > > > > > > Douglas D. Deltour HT(ASCP) > > Histology Manager > > Professional Pathology Services, PC > > One Science Court > > Suite 200 > > Columbia, SC 29203 > > Office (803)252-1913 > > Fax (803)254-3262 > > Doug@ppspath.com > > ***************************************************** > > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of > the individual or entity to > which it is addressed and may contain > information that is privileged, > confidential and exempt from disclosure under > applicable law. If the reader > of this message is not the intended recipient, > you are hereby notified that > any dissemination, distribution, or copying of > this communication is > strictly prohibited by law. If you have > received this communication in > error, please notify me immediately. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D ____________________________________________________________________________ ________ Luggage? GPS? Comic books? Check out fitting gifts for grads at Yahoo! Search http://search.yahoo.com/search?fr=oni_on_mail&p=graduation+gifts&cs=bz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From m5johnso <@t> meded.ucsd.edu Thu Sep 6 12:28:08 2007 From: m5johnso <@t> meded.ucsd.edu (Mindy Johnson) Date: Thu Sep 6 12:28:36 2007 Subject: {SPAM?} RE: [Histonet] Job Opening (Columbia SC) In-Reply-To: <20070906172418.796HOIm00E8DC@mailbox5.ucsd.edu> References: <559818.86032.qm@web52505.mail.re2.yahoo.com> <20070906172418.796HOIm00E8DC@mailbox5.ucsd.edu> Message-ID: <01bf01c7f0ab$50eece50$f2cc6af0$@ucsd.edu> How bad are the mosquitoes out there with that pond so close? ?Mindy A Johnson SRA II UCSD - School of Medicine Medical Teaching Labs Fax: 858-822-5250 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Thursday, September 06, 2007 11:19 AM To: 'Patricia Adams'; 'HistoNet' Subject: RE: {SPAM?} RE: [Histonet] Job Opening (Columbia SC) Ducks and turtles! Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patricia Adams Sent: Thursday, September 06, 2007 12:11 PM To: Douglas D Deltour; HistoNet Subject: {SPAM?} RE: [Histonet] Job Opening (Columbia SC) Swans? --- Douglas D Deltour wrote: > Of course :) > > Douglas D. Deltour HT(ASCP) > Histology Manager > Professional Pathology Services, PC > One Science Court > Suite 200 > Columbia, SC 29203 > Office (803)252-1913 > Fax (803)254-3262 > Doug@ppspath.com > ***************************************************** > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of > the individual or entity to > which it is addressed and may contain > information that is privileged, > confidential and exempt from disclosure under > applicable law. If the reader > of this message is not the intended recipient, > you are hereby notified that > any dissemination, distribution, or copying of > this communication is > strictly prohibited by law. If you have > received this communication in > error, please notify me immediately. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Blazek, > Linda > Sent: Thursday, September 06, 2007 11:56 AM > To: Douglas D Deltour; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Job Opening (Columbia > SC) > > Is the pond lighted? > > Linda Blazek HT (ASCP) > Manager/Supervisor > GI Pathology of Dayton > 7415 Brandt Pike > Huber Heights, OH 45424 > Phone: (937) 293-4424 ext 7118 > Email: lblazek@digestivespecialists.com > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Douglas D > Deltour > Sent: Thursday, September 06, 2007 9:32 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Job Opening (Columbia SC) > > Professional Pathology Services, PC is one of > the regions fastest growing > Pathology laboratories located in sunny > Columbia South Carolina. Our state > of the art histology laboratory has a wall of > windows with a scenic view of > our own pond. Come join our fun and exciting > team of healthcare > professionals. > > > > We are seeking a full-time certified histology > technician > > ASCP certified w/1-5 years of experience in > Histology > > New Grads welcome to apply > This position is for the hours of 9:30PM-6:00AM > > NO RECRUITERS PLEASE > > Send r?sum? to Doug@ppspath.com > www.ppspath.com > > > > > > Douglas D. Deltour HT(ASCP) > > Histology Manager > > Professional Pathology Services, PC > > One Science Court > > Suite 200 > > Columbia, SC 29203 > > Office (803)252-1913 > > Fax (803)254-3262 > > Doug@ppspath.com > > ***************************************************** > > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of > the individual or entity to > which it is addressed and may contain > information that is privileged, > confidential and exempt from disclosure under > applicable law. If the reader > of this message is not the intended recipient, > you are hereby notified that > any dissemination, distribution, or copying of > this communication is > strictly prohibited by law. If you have > received this communication in > error, please notify me immediately. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D ____________________________________________________________________________ ________ Luggage? GPS? Comic books? Check out fitting gifts for grads at Yahoo! Search http://search.yahoo.com/search?fr=oni_on_mail&p=graduation+gifts&cs=bz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Thu Sep 6 12:30:17 2007 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Thu Sep 6 12:30:32 2007 Subject: [Histonet] IHC staining weaker in - P.S. In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E273CDA@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: I concur with Paul on this one...sounds like a fixation problem. Glen Dawson IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Monfils, Paul Sent: Thursday, September 06, 2007 11:38 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC staining weaker in - P.S. P.S. Of course, even with a faster penetrating fixative like formalin, the same artifact can be produced simply by insufficient time in the fixative to allow full penetration. Are you seeing this artifact mostly in larger tissue sections? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MBURTON1 <@t> PARTNERS.ORG Thu Sep 6 12:38:03 2007 From: MBURTON1 <@t> PARTNERS.ORG (Burton, Mark) Date: Thu Sep 6 12:38:18 2007 Subject: [Histonet] Job Opening (Columbia SC) In-Reply-To: <65e977$mdcl5@phsmgmx9.partners.org> Message-ID: Make sure you click the hybrid button on the Google map. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Thursday, September 06, 2007 2:15 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Job Opening (Columbia SC) For all of you that found the "pond" amusing here you go. :) http://maps.google.com/?ie=UTF8&ll=34.099354,-80.964872&spn=0.00239,0.003648 &t=k&z=18&om=1 Bring your fishing pole! Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Thursday, September 06, 2007 11:56 AM To: Douglas D Deltour; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Job Opening (Columbia SC) Is the pond lighted? Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Thursday, September 06, 2007 9:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Job Opening (Columbia SC) Professional Pathology Services, PC is one of the regions fastest growing Pathology laboratories located in sunny Columbia South Carolina. Our state of the art histology laboratory has a wall of windows with a scenic view of our own pond. Come join our fun and exciting team of healthcare professionals. We are seeking a full-time certified histology technician ASCP certified w/1-5 years of experience in Histology New Grads welcome to apply This position is for the hours of 9:30PM-6:00AM NO RECRUITERS PLEASE Send r?sum? to Doug@ppspath.com www.ppspath.com Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From doug <@t> ppspath.com Thu Sep 6 13:38:50 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Thu Sep 6 12:39:28 2007 Subject: {SPAM?} RE: [Histonet] Job Opening (Columbia SC) In-Reply-To: <01bf01c7f0ab$50eece50$f2cc6af0$@ucsd.edu> Message-ID: Beats me? I usually don't go sun-bathing by the pond. :) Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mindy Johnson Sent: Thursday, September 06, 2007 12:28 PM To: 'Douglas D Deltour'; 'Patricia Adams'; 'HistoNet' Subject: RE: {SPAM?} RE: [Histonet] Job Opening (Columbia SC) How bad are the mosquitoes out there with that pond so close? ?Mindy A Johnson SRA II UCSD - School of Medicine Medical Teaching Labs Fax: 858-822-5250 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Thursday, September 06, 2007 11:19 AM To: 'Patricia Adams'; 'HistoNet' Subject: RE: {SPAM?} RE: [Histonet] Job Opening (Columbia SC) Ducks and turtles! Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patricia Adams Sent: Thursday, September 06, 2007 12:11 PM To: Douglas D Deltour; HistoNet Subject: {SPAM?} RE: [Histonet] Job Opening (Columbia SC) Swans? --- Douglas D Deltour wrote: > Of course :) > > Douglas D. Deltour HT(ASCP) > Histology Manager > Professional Pathology Services, PC > One Science Court > Suite 200 > Columbia, SC 29203 > Office (803)252-1913 > Fax (803)254-3262 > Doug@ppspath.com > ***************************************************** > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of > the individual or entity to > which it is addressed and may contain > information that is privileged, > confidential and exempt from disclosure under > applicable law. If the reader > of this message is not the intended recipient, > you are hereby notified that > any dissemination, distribution, or copying of > this communication is > strictly prohibited by law. If you have > received this communication in > error, please notify me immediately. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Blazek, > Linda > Sent: Thursday, September 06, 2007 11:56 AM > To: Douglas D Deltour; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Job Opening (Columbia > SC) > > Is the pond lighted? > > Linda Blazek HT (ASCP) > Manager/Supervisor > GI Pathology of Dayton > 7415 Brandt Pike > Huber Heights, OH 45424 > Phone: (937) 293-4424 ext 7118 > Email: lblazek@digestivespecialists.com > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Douglas D > Deltour > Sent: Thursday, September 06, 2007 9:32 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Job Opening (Columbia SC) > > Professional Pathology Services, PC is one of > the regions fastest growing > Pathology laboratories located in sunny > Columbia South Carolina. Our state > of the art histology laboratory has a wall of > windows with a scenic view of > our own pond. Come join our fun and exciting > team of healthcare > professionals. > > > > We are seeking a full-time certified histology > technician > > ASCP certified w/1-5 years of experience in > Histology > > New Grads welcome to apply > This position is for the hours of 9:30PM-6:00AM > > NO RECRUITERS PLEASE > > Send r?sum? to Doug@ppspath.com > www.ppspath.com > > > > > > Douglas D. Deltour HT(ASCP) > > Histology Manager > > Professional Pathology Services, PC > > One Science Court > > Suite 200 > > Columbia, SC 29203 > > Office (803)252-1913 > > Fax (803)254-3262 > > Doug@ppspath.com > > ***************************************************** > > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of > the individual or entity to > which it is addressed and may contain > information that is privileged, > confidential and exempt from disclosure under > applicable law. If the reader > of this message is not the intended recipient, > you are hereby notified that > any dissemination, distribution, or copying of > this communication is > strictly prohibited by law. If you have > received this communication in > error, please notify me immediately. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D ____________________________________________________________________________ ________ Luggage? GPS? Comic books? Check out fitting gifts for grads at Yahoo! Search http://search.yahoo.com/search?fr=oni_on_mail&p=graduation+gifts&cs=bz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Thu Sep 6 12:49:00 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Thu Sep 6 12:49:18 2007 Subject: [Histonet] re: caspase 3 cleaved Message-ID: <000a01c7f0ae$352a9f70$4101a8c0@carlba65530bda> Many thanks for information. Much appreciated Carl From lfvest <@t> vt.edu Thu Sep 6 12:50:46 2007 From: lfvest <@t> vt.edu (Vest, Luther) Date: Thu Sep 6 12:50:58 2007 Subject: [Histonet] protocol for clearing tissue! Message-ID: Dear histonet: One of the pathology residents in our department has asked me to post this question. I am in search of a protocol for clearing tissue. This technique is used commonly in vascular research to visualize blood vessels. I want to use it to clear mouse lung tissue that contains blue-stained (B-galactosidase activity) tumor nodules. Any suggestions? Thanks Luther Vest (HT ASCP) VA-MD Regional College of Veterinary Medicine Duckpond Dr. Phase III Blacksburg, Va 24061-0443 From Doug.Geddes <@t> lhsc.on.ca Thu Sep 6 13:14:38 2007 From: Doug.Geddes <@t> lhsc.on.ca (Doug Geddes) Date: Thu Sep 6 13:15:17 2007 Subject: [Histonet] Microwaves VENDOR RESPONSE In-Reply-To: <34BB307EFC9A65429BBB49E330675F7202BDBFEE@LTA3VS003.ees.hhs.gov > References: <46DD8689.4AA8.00C0.0@ah.org><46DEB823.4060708@ebsciences.com><0 03b01c7efd5$52d0da00$d49eae18@yourxhtr8hvc4p> <46DFE8F9.6E1B.0061.0@lhsc.on.ca><34BB307EFC9A65429BBB49E330675F7202BDBFEE@ LTA3VS003.ees.hhs.gov> Message-ID: <46E00B4D.6E1B.0061.0@lhsc.on.ca> At present we are using the TT Mega for antigen retrieval only. Doug Geddes BSc, MLT Department of Pathology London Helath Sciences Centre London, ON Canada >>> "Bartlett, Jeanine (CDC/CCID/NCZVED)" 2007-09-06 11:58 AM >>> Do you use the TT/Mega just for special staining? Jeanine Bartlett Infectious Disease Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Doug Geddes Sent: Thursday, September 06, 2007 11:48 AM To: Kathleen Boozer; Phil McArdle; Joe Nocito Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Microwaves VENDOR RESPONSE Sorry Joe, but budgets aside, I think you get what you pay for. We are a high volume lab and have gone through at least 8 "Wal Mart" microwaves in the last 5 years. We now have the TT Mega, and it is wonderful, it is all about control as much as you can. Morphology is amazing. Doug Geddes BSc, MLT Department of Pathology London Helath Sciences Centre London, ON Canada >>> "Joe Nocito" 2007-09-05 11:56 AM >>> ok, but with the budgets today, many people can't afford a $1749 microwave when they can buy one at Walmart, K-Mart, or somewhere else for $79. Not to make you angry or anything, but I'm wondering how long has it been since you worked in a lab? Histo's budget is the first one cut in the lab because we are not essential. I can't count how many times I fought and fought for my budgets. If I tried to justify a $1749 microwave for special stains, HIER or whatever, I would have been laughed out the manager's office. Just my 4 cents. JTT ----- Original Message ----- From: "Phil McArdle" To: "Kathleen Boozer" Cc: Sent: Wednesday, September 05, 2007 9:07 AM Subject: Re: [Histonet] Microwaves VENDOR RESPONSE > Again, a microwave vendor weighs in (so far I haven't received any > flames), so read at your own risk. :-) > > At the risk of sounding overly and overtly commercial, after reading post > after post of $30,000+ and $18,000 and similarly high figures for lab > microwaves, I really feel the need to set the record straight. Depending > on the usage requirements, we have laboratory microwaves as low as $1749 > for a "bare bones" model for simple operations, to mid-priced units, to > under $11,000 for a vacuum equipped microwave processor capable of the +/- > 0.5 degree C temperature control necessary for tissue processing. > > (I can feel the heat already!) > > There are many compelling reasons to replace a kitchen microwave with a > lab model; feel free to download, read, and even share with colleagues our > Microwave Companion at > > http://www.ebsciences.com/pdf/EBS_MW_COMPANION.pdf > > Best regards, and see you at NSH, > > Phil McArdle > > -- > Phil McArdle > Microwave Product Manager > > Energy Beam Sciences, Inc. > 29-B Kripes Rd. > East Granby, CT 06026 > > Tel: 800.992.9037 x 341 > Mobile: 860.597.6796 > Fax: 860.653.0422 > > pmcardle@ebsciences.com > www.ebsciences.com > > Kathleen Boozer wrote: >> What is the best microwave for a small lab using it only for heating >> Bouin's and Silver Nitrate for special stains? I just can't believe I >> would have to spend $30,000+ or slow down and use a waterbath. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > I skate to where the puck is going to be, not to where it's been. > - Wayne Gretsky > > You must be the change you want to see in the world. > - Mahatma Gandhi > > NOTE: This message, together with any attachments, is intended only for > the use of the individual or entity to which it is addressed and may > contain information that is legally privileged, confidential and exempt > from disclosure. If you are not the intended recipient, however, there's > not a lot I can do about it, and it was probably my mistake anyway. So > please do the right thing and make this e-mail go away. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alaskagirl1950 <@t> yahoo.com Thu Sep 6 13:25:26 2007 From: alaskagirl1950 <@t> yahoo.com (Patricia Adams) Date: Thu Sep 6 13:25:39 2007 Subject: [Histonet] Job Opening (Columbia SC) Message-ID: <287998.99715.qm@web52506.mail.re2.yahoo.com> Looks like a woman's high heel low boot! --- Douglas D Deltour wrote: > For all of you that found the "pond" amusing > here you go. :) > > http://maps.google.com/?ie=UTF8&ll=34.099354,-80.964872&spn=0.00239,0.003648 > &t=k&z=18&om=1 > > Bring your fishing pole! > > Douglas D. Deltour HT(ASCP) > Histology Manager > Professional Pathology Services, PC > One Science Court > Suite 200 > Columbia, SC 29203 > Office (803)252-1913 > Fax (803)254-3262 > Doug@ppspath.com > ***************************************************** > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of > the individual or entity to > which it is addressed and may contain > information that is privileged, > confidential and exempt from disclosure under > applicable law. If the reader > of this message is not the intended recipient, > you are hereby notified that > any dissemination, distribution, or copying of > this communication is > strictly prohibited by law. If you have > received this communication in > error, please notify me immediately. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Blazek, > Linda > Sent: Thursday, September 06, 2007 11:56 AM > To: Douglas D Deltour; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Job Opening (Columbia > SC) > > Is the pond lighted? > > Linda Blazek HT (ASCP) > Manager/Supervisor > GI Pathology of Dayton > 7415 Brandt Pike > Huber Heights, OH 45424 > Phone: (937) 293-4424 ext 7118 > Email: lblazek@digestivespecialists.com > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Douglas D > Deltour > Sent: Thursday, September 06, 2007 9:32 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Job Opening (Columbia SC) > > Professional Pathology Services, PC is one of > the regions fastest growing > Pathology laboratories located in sunny > Columbia South Carolina. Our state > of the art histology laboratory has a wall of > windows with a scenic view of > our own pond. Come join our fun and exciting > team of healthcare > professionals. > > > > We are seeking a full-time certified histology > technician > > ASCP certified w/1-5 years of experience in > Histology > > New Grads welcome to apply > This position is for the hours of 9:30PM-6:00AM > > NO RECRUITERS PLEASE > > Send r?sum? to Doug@ppspath.com > www.ppspath.com > > > > > > Douglas D. Deltour HT(ASCP) > > Histology Manager > > Professional Pathology Services, PC > > One Science Court > > Suite 200 > > Columbia, SC 29203 > > Office (803)252-1913 > > Fax (803)254-3262 > > Doug@ppspath.com > > ***************************************************** > > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of > the individual or entity to > which it is addressed and may contain > information that is privileged, > confidential and exempt from disclosure under > applicable law. If the reader > of this message is not the intended recipient, > you are hereby notified that > any dissemination, distribution, or copying of > this communication is > strictly prohibited by law. If you have > received this communication in > error, please notify me immediately. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D ____________________________________________________________________________________ Park yourself in front of a world of choices in alternative vehicles. Visit the Yahoo! Auto Green Center. http://autos.yahoo.com/green_center/ From Barry.R.Rittman <@t> uth.tmc.edu Thu Sep 6 14:08:31 2007 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Thu Sep 6 14:08:49 2007 Subject: [Histonet] protocol for clearing tissue! In-Reply-To: Message-ID: Oil of wintergreen has been used extensively in the past for clearing even large blocks of tissue containing blood vessels visualized with India ink, latex etc. So this would probably work. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vest, Luther Sent: Thursday, September 06, 2007 12:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] protocol for clearing tissue! Dear histonet: One of the pathology residents in our department has asked me to post this question. I am in search of a protocol for clearing tissue. This technique is used commonly in vascular research to visualize blood vessels. I want to use it to clear mouse lung tissue that contains blue-stained (B-galactosidase activity) tumor nodules. Any suggestions? Thanks Luther Vest (HT ASCP) VA-MD Regional College of Veterinary Medicine Duckpond Dr. Phase III Blacksburg, Va 24061-0443 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bitesizellama <@t> gmail.com Thu Sep 6 14:41:51 2007 From: bitesizellama <@t> gmail.com (Mauricio Avigdor) Date: Thu Sep 6 14:42:03 2007 Subject: [Histonet] Acetone fixation for immunofluorescence protocols Message-ID: <1b2831cd0709061241k57f14da7n121a9b8f58912b04@mail.gmail.com> Greetings: I am having a bit of difficulty with an immunofluorescence protocol (IF). I am using fresh tissue fixed for 10 minutes in cold acetone. Are there any fixatives that are well suited to IF protocols? Has anyone attempted to stain unfixed tissue? From wstover <@t> vet.uga.edu Thu Sep 6 13:02:32 2007 From: wstover <@t> vet.uga.edu (Wanda Stover) Date: Thu Sep 6 14:46:49 2007 Subject: [Histonet] Counterstain for GFP tagged Listeria Message-ID: Does anyone know a counterstain we can use for GFP tagged Listeria? The researcher wants something to make the fluorescent show up better. The slides are routine paraffin sections. Help? Wanda Stover UGA VET MED Histology Athens, Ga. 30602 706 583-0474 From jnocito <@t> satx.rr.com Thu Sep 6 15:30:27 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Sep 6 15:30:59 2007 Subject: [Histonet] Microwaves VENDOR RESPONSE References: <46DD8689.4AA8.00C0.0@ah.org> <46DEB823.4060708@ebsciences.com> <46DFE8F9.6E1B.0061.0@lhsc.on.ca> Message-ID: <007001c7f0c4$c826f100$0202a8c0@yourxhtr8hvc4p> Doug, if you are doing a high enough volume, I can see it. In my old lab we might microwave maybe 6-8 times on a heavy day. Joe ----- Original Message ----- From: "Doug Geddes" To: "Kathleen Boozer" ; "Phil McArdle" ; "Joe Nocito" Cc: Sent: Thursday, September 06, 2007 10:48 AM Subject: Re: [Histonet] Microwaves VENDOR RESPONSE Sorry Joe, but budgets aside, I think you get what you pay for. We are a high volume lab and have gone through at least 8 "Wal Mart" microwaves in the last 5 years. We now have the TT Mega, and it is wonderful, it is all about control as much as you can. Morphology is amazing. Doug Geddes BSc, MLT Department of Pathology London Helath Sciences Centre London, ON Canada >>> "Joe Nocito" 2007-09-05 11:56 AM >>> ok, but with the budgets today, many people can't afford a $1749 microwave when they can buy one at Walmart, K-Mart, or somewhere else for $79. Not to make you angry or anything, but I'm wondering how long has it been since you worked in a lab? Histo's budget is the first one cut in the lab because we are not essential. I can't count how many times I fought and fought for my budgets. If I tried to justify a $1749 microwave for special stains, HIER or whatever, I would have been laughed out the manager's office. Just my 4 cents. JTT ----- Original Message ----- From: "Phil McArdle" To: "Kathleen Boozer" Cc: Sent: Wednesday, September 05, 2007 9:07 AM Subject: Re: [Histonet] Microwaves VENDOR RESPONSE > Again, a microwave vendor weighs in (so far I haven't received any > flames), so read at your own risk. :-) > > At the risk of sounding overly and overtly commercial, after reading post > after post of $30,000+ and $18,000 and similarly high figures for lab > microwaves, I really feel the need to set the record straight. Depending > on the usage requirements, we have laboratory microwaves as low as $1749 > for a "bare bones" model for simple operations, to mid-priced units, to > under $11,000 for a vacuum equipped microwave processor capable of the +/- > 0.5 degree C temperature control necessary for tissue processing. > > (I can feel the heat already!) > > There are many compelling reasons to replace a kitchen microwave with a > lab model; feel free to download, read, and even share with colleagues our > Microwave Companion at > > http://www.ebsciences.com/pdf/EBS_MW_COMPANION.pdf > > Best regards, and see you at NSH, > > Phil McArdle > > -- > Phil McArdle > Microwave Product Manager > > Energy Beam Sciences, Inc. > 29-B Kripes Rd. > East Granby, CT 06026 > > Tel: 800.992.9037 x 341 > Mobile: 860.597.6796 > Fax: 860.653.0422 > > pmcardle@ebsciences.com > www.ebsciences.com > > Kathleen Boozer wrote: >> What is the best microwave for a small lab using it only for heating >> Bouin's and Silver Nitrate for special stains? I just can't believe I >> would have to spend $30,000+ or slow down and use a waterbath. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > I skate to where the puck is going to be, not to where it's been. > - Wayne Gretsky > > You must be the change you want to see in the world. > - Mahatma Gandhi > > NOTE: This message, together with any attachments, is intended only for > the use of the individual or entity to which it is addressed and may > contain information that is legally privileged, confidential and exempt > from disclosure. If you are not the intended recipient, however, there's > not a lot I can do about it, and it was probably my mistake anyway. So > please do the right thing and make this e-mail go away. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Thu Sep 6 15:32:40 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Sep 6 15:32:56 2007 Subject: [Histonet] Acetone fixation for immunofluorescence protocols References: <1b2831cd0709061241k57f14da7n121a9b8f58912b04@mail.gmail.com> Message-ID: <007601c7f0c5$12955ab0$0202a8c0@yourxhtr8hvc4p> Mauricio, I cut my tissue and let it air dry for 30 minutes, then acetone fix for 30 minutes at room temperature, then 3 changes of PBS for 5 minutes each before applying the antibody and I rarely have problems. JTT ----- Original Message ----- From: "Mauricio Avigdor" To: Sent: Thursday, September 06, 2007 2:41 PM Subject: [Histonet] Acetone fixation for immunofluorescence protocols > Greetings: > > I am having a bit of difficulty with an immunofluorescence protocol (IF). > I > am using fresh tissue fixed for 10 minutes in cold acetone. Are there any > fixatives that are well suited to IF protocols? Has anyone attempted to > stain unfixed tissue? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Thu Sep 6 15:34:47 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Sep 6 15:35:14 2007 Subject: [Histonet] IHC staining weaker in... References: <407F05A128805F4C879A33DBA32E618E0189502E@TRFT-EX01.xRothGen.nhs.uk> Message-ID: <008901c7f0c5$5ec41200$0202a8c0@yourxhtr8hvc4p> sounds like a fixation problem. Joe ----- Original Message ----- From: "Marshall Terry Dr, Consultant Histopathologist" To: "Department of Pathology" ; Sent: Thursday, September 06, 2007 11:14 AM Subject: RE: [Histonet] IHC staining weaker in... I thought I had seen every horror possible in a section, but that seems a new one to me! Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Department of Pathology Sent: 06 September 2007 16:48 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC staining weaker in... Dear Participants, our diagnostic pathology lab has a problem. The paraffin sections are stained weakly at the center but better at the periphery of the sections. What could be the reason.??... thanks a lot.. Dr Nasuhi Engin Aydin, malatya Inonu University Hospital, Turkey 44365. ---------- Original Message ----------- From: histonet-request@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Thu, 6 Sep 2007 14:51:24 +0300 (EEST) Subject: Histonet Digest, Vol 46, Issue 6 > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > Today's Topics: > > 1. Xylene (themagoos) > 2. RE:The great apoptosis stains debate (TUNEL, Caspases, etc) > (Melissa Gonzalez) > 3. Re: RDO decalcifier (Robert Richmond) > 4. Re: IHC anti-cleaved caspase 3 (Carl Hobbs) > 5. avian sperm cell topography (melissah rowe) > 6. Re: IHC anti-cleaved caspase 3 (Thomas Pier) > 7. Re: IHC anti-cleaved caspase 3 (Cheryl Cross) > 8. Fwd: Users of Lab Vision autostainer (Victoria Baker) > 9. Re: Microwaves VENDOR RESPONSE (Phil McArdle) > 10. Re: Xylene (Rene J Buesa) > 11. RE: IHC anti-cleaved caspase 3 (Liz Chlipala) > 12. Freezing mouse testes for frozen sections > (Sarah Clatterbuck Soper) > 13. Olympus BX40 (Mike Pence) > 14. Look for a used stereoscope (Yu, Jian) > 15. RE: RDO decal (Tony Henwood) > 16. PTH antibody reacted with dog tissue... (ChoiUl Soo) > 17. Floater sources (Michelle McCoy) > 18. RE: Xylene (Kemlo Rogerson) > 19. RE: Floater sources (Kemlo Rogerson) > 20. Fwd: Users of Lab Vision autostainer (Victoria Baker) > 21. RE: Xylene (Joseph Kapler) > 22. Unsubscribe (Malam Jacqueline) > 23. Re: Floater sources (Joe Nocito) > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 05 Sep 2007 10:21:51 -0700 > From: "themagoos" > Subject: [Histonet] Xylene > To: histonet@lists.utsouthwestern.edu > Message-ID: <46dee5af.14b.450a.1030817715@rushmore.com> > Content-Type: text/plain; charset="iso-8859-1" > > Can somebody tell me if there are any effects on tissue if > there is prolonged processing time in xylene? > > Jason McGough HT(ASCP) > Clinical Laboratory of the Black Hills > Account Representative - Anatomic Pathology > 2805 5th Street > Rapid City, SD 57701 > 605-343-2267 > jmcgough@clinlab.com > > ------------------------------ > > Message: 2 > Date: Wed, 5 Sep 2007 11:30:38 -0700 > From: "Melissa Gonzalez" > Subject: [Histonet] RE:The great apoptosis stains debate (TUNEL, > Caspases, etc) > To: > Message-ID: > <2884B897182A1D438C7BA24B9A8F94A20E701D@hqsvr01mail.cgi.com> > Content-Type: text/plain; charset="iso-8859-1" > > Hi all, > I have been around this mess a few years back, I gave up on TUNEL long ago > for > the various reasons mentioned on the list recently. > > Per investigators requests, I have tried several ways to demonstrate cell > death, however methods such as PI or Annexin staining are more suitable > for > whole cells, not tissue sections. I have also not had any luck staining > for > Cytochrome C. > > We routinely use R&D Systems rabbit anti human/mouse active Caspase3 using > a > high pH (EDTA) based Ag retrieval in a steamer (enzymes do not work). > Works > like a charm. > > Any other suggestions? I know this topic has been thrown around a lot, but > these discussions after all, help us get our jobs done more proficiently. > > Melissa > > Melissa A. Gonz?lez Edick > R&D, Cell Genesys Inc. > 500 Forbes Blvd > South San Francisco, CA 94080 > p(650) 266-3168 > f (650) 266-3080 > > "It's not enough to believe what you see, you must also understand what > you > see." -Leonardo Da Vinci > ------------------------------ > > Message: 6 > Date: Tue, 4 Sep 2007 15:18:39 -0400 > From: Cheryl Cross > Subject: Re: [Histonet] TUNEL, Formalin, DNA strand breaks > To: JR R > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <4C1E074D-064A-45D1-BD32-13D7E2222B26@aol.com> > Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed > > Hi all - > > I have been round and round with this issue myself; I was basically > told by people who've attempted it that TUNEL on FFPE sections is not > specific enough due to the formalin issue. > > If you are trying to nail apoptosis, what about anti-active > caspase-3? mind you, that staining can be a bit of a booger too, but > it should be more sensitive and specific for apoptosis (no references > to offer, i'm just repeating what i have been told). > > Cheryl Cross, DVM, Dipl. ACVP > Researcher > University Corporation for Atmospheric Research > College of Veterinary Medicine > University of Tennessee Department of Pathology > 2407 River Drive, Room A201 > Knoxville, TN 37996-4542 > (423) 967-2724 > fax: 865-974-5616 > ccross@ucar.edu > ------------------------------ > > Message: 5 > Hi Ray, > > Sheesh-- I read (and then lost) the paper maybe 5-7 years ago, back when I > was > tearing my hair out over high background, or false positives for TUNEL > stain > in formalin fixed, paraffin embedded arterial sections. I can > troubleshoot > any immunostain, but the TUNEL and ISEL assays bedeviled me. > > The paper was titled something to the effect of "formaldehyde causes > single > and double stranded DNA breaks," and was pretty emphatic. I'll look for > the article. > > I think the assay could work in whole cells for flow, or maybe in frozen > tissue. My hunch at this point is that this is one of those rare cases > where > lots and lots of peer reviewed articles are just plain wrong. > > Hey, if someone has a good protocol, I'd be willing to try it out, and I > would > be delighted if I turn out to be mistaken. For now, I think TUNEL is the > assay of the beast. > > Oh, here is a thought--say you are looking at a 5 micron section through a > nucleus. The microtome blade pretty much had to create a lot of double > stranded DNA breaks, no? > > Jerry L. Ricks > Research Scientist > U.W. Medicine at South Lake Union > 815 Mercer Street > Seattle, WA 98109 > (206)-685-7190 > > From: koellingr@comcast.netTo: rosenfeldtek@hotmail.com; > histonet@lists.utsouthwestern.eduSubject: RE: [Histonet] TUNELDate: Fri, > 31 > Aug 2007 23:17:03 +0000 > > Jerry, > Could you expand on or give references to formalin causing DNA strand > breaks? > Double strand breaks, single strand, blunt end, overhanging? My pile of > papers and having done TUNEL for years says that formalin fixation is a > very > good technique for TUNEL and many peer-reviewed articles in which TUNEL is > used as a technique, use formalin fixation and how can that be if formalin > is > causing strand breaks? In fact one paper I'm looking at says that > extended (5- > 7 weeks in formalin) fixation causes loss of TUNEL signal. If formalin is > causing breaks, you would assume that TUNEL pos signals would increase > with > extended formalin fixation. Even the use of the monoclonal antibody > F7-26, > for single stranded DNA, touts formalin fixation for their claims of > discriminating apoptosis from necrosis. > > The question asks about extended alcohol fixation but your answer is > possibly > a lot of false positives because formalin causes DNA breaks. Does this > imply > that alcohol won't? Have done a lot of TUNEL on alcohol fixed samples. > True > I couldn't pretreat them and handle them they way I would handle FFPE > tissue. > Also true that we could argue specificity and ability or not to > discriminate > apoptosis from necrosis for quite a while. But if formalin itself is > causing > the breaks in DNA, I and a lot of people are in big trouble with our > science > projects and experiments. Also I can't envision why 2 cells are showing > TUNEL > positivity while 2 of the same type of cells right next to them (and > getting > the same formalin fix), are absolutely clean and there is no background? > > Thanks for any information you can provide. > > Ray Koelling > PhenoPath Laboratories > Seattle, WA > > ------------------------------ > > Message: 3 > Date: Wed, 5 Sep 2007 14:56:14 -0400 > From: "Robert Richmond" > Subject: [Histonet] Re: RDO decalcifier > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > I don't see how your hazmats people can tell you what to do to dispose > of RDO, since nobody has any idea what's in it. It's a murky yellow > liquid that continuously throws a black sediment that has to be > filtered out if you're to see your specimen in it. It's sort of the > ultimate secret formula, and I've always wondered why it's so popular > - I've had to use it many different places in my travels. > > I'd suggest either an ordinary proprietary decalcifier that's a clear > liquid, probably hydrochloric acid - or else save money and dilute > your own hydrochloric acid. You can re-use it for a while. When the > time comes to dispose of it, it can be safely diluted with a lot of > water and put down the drain. > > Remember - it's been stressed on this list many times - that "Decal" > is a brand name still in use, and that other brands of decalcifying > solution should not be called Decal. > > Bob Richmond > Samurai Pathologist (never a decalcified Carmelite) > Knoxville TN > > ------------------------------ > > Message: 4 > Date: Wed, 5 Sep 2007 20:29:36 +0100 > From: "Carl Hobbs" > Subject: Re: [Histonet] IHC anti-cleaved caspase 3 > To: "Histonet" > Message-ID: <001801c7eff3$18894850$4101a8c0@carlba65530bda> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > Hi. > > Recent Cleaved caspase 3 posts have not mentioned the antibody details: I > would be grateful for the source of these Abs. > Carl > > ------------------------------ > > Message: 5 > Date: Wed, 5 Sep 2007 14:36:50 -0500 > From: melissah rowe > Subject: [Histonet] avian sperm cell topography > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed > > Hi, > > I am a PhD student working on a behavioral ecology project on > Australian fairy-wrens. I am currently trying to find out if I can > determine the dimensions of discrete sections of individual sperm > cells, i.e. acrosome length, nucleus length, midpiece length and tail > length using the material I currently have available. I have prepared > slide smears of sperm that has been previously stained with an eosin- > nigrosin stain (to determine cell viability) and would like to be > able to somehow stain different segments of the sperm so that I can > identify and measure them using an ocular micrometer on a bright > field or phase contrast microscope set up. It is my understanding > that I cannot use fluorescent stains given that I have already used a > colormetric stain (the eosin-nigrosin). If anyone has suggestions for > ways to identify discrete regions of avian (passerine) sperm cells I > would be very grateful to hear from you. > > Many thanks in advance, > melissah > > ----- > melissah rowe > > PhD candidate > Department of Ecology & Evolution > University of Chicago > E. 57th Street, Chicago, IL, 60637 > ph: +1 773-702-3070 > fax: + 1 773-702-9740 > > email: melissah@uchicago.edu > > ------------------------------ > > Message: 6 > Date: Wed, 05 Sep 2007 14:39:04 -0500 > From: "Thomas Pier" > Subject: Re: [Histonet] IHC anti-cleaved caspase 3 > To: , > Message-ID: <46DEBF88020000DF0000A736@gwmail.medicine.wisc.edu> > Content-Type: text/plain; charset=US-ASCII > > Cell Signalling Technology has a good rabbit monoclonal for Cleaved > Caspase-3. > > Tom Pier > > >>> "Carl Hobbs" 09/05/07 2:29 PM >>> > Hi. > > Recent Cleaved caspase 3 posts have not mentioned the antibody details: I > would be grateful for the source of these Abs. > Carl > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 7 > Date: Wed, 5 Sep 2007 15:54:35 -0400 > From: Cheryl Cross > Subject: Re: [Histonet] IHC anti-cleaved caspase 3 > To: "Thomas Pier" > Cc: carl.hobbs@kcl.ac.uk, histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed > > I will second the Cell Signaling antibody - we have tried antibodies > from Promega (which gave tons of background staining)...then via this > site i was pointed to Cell Signaling's monoclonal which we tried; the > polyclonal actually has been working very well with minimal > background (we were getting lots of respiratory epithelium and > endothelium lighting up in control animals). I have been using it in > mice with this protocol: > > EDTA with PASCAL > primary antibody 60 minutes, dilution 1:125 > Rabbit envision > DAB plus > > Hope this helps! > > Cheryl Cross, DVM, Dipl. ACVP > Researcher > University Corporation for Atmospheric Research > College of Veterinary Medicine > University of Tennessee Department of Pathology > 2407 River Drive, Room A201 > Knoxville, TN 37996-4542 > (423) 967-2724 > fax: 865-974-5616 > ccross@ucar.edu > > > > > ------------------------------ > > Message: 8 > Date: Wed, 5 Sep 2007 15:59:45 -0400 > From: "Victoria Baker" > Subject: [Histonet] Fwd: Users of Lab Vision autostainer > To: "Histo Net list server" > Message-ID: > <4f016b690709051259rcdffccet70d7f97ae0457707@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > One more try, first one didn't go through it seems! Thanks > > ---------- Forwarded message ---------- > From: Victoria Baker > Date: Sep 5, 2007 9:02 AM > Subject: Users of Lab Vision autostainer > To: Histo Net list server > > Hi > > I'm a new user of the Lab Vision autostainer and I'm looking to see if > I can find users in Histo-land that have experience with it. The > facility only works with human tissue and all of the antibodies are > for dx purposes. > > My key questions are as follows: > How many antibodies is your lab running? > How many users do you allow? > How many people do you allow programming rights and at what level of > supervision are they? > > How many of these antibodies are from Lab Vision? > a) are they concentrates or pre-dilutes? > b) for HIER are you using their PT modules/procedures or > your own equipment (microwave, steamer, pressure cooker etc) and in > house designed protocols for retrieval? > c) for digestion do you only use their Pro-K or have you > designed your own in-house methods using other reagents for digestion? > d) do you put your controls on the same slide as the patient? > e) are your controls in-house or commercial? > f) do you have more than one stainer hooked up to one computer > system? > > What Version of software do you currently have on your system? > > Any feed back would be very helpful. > > Thanks in advance. > > Vikki Baker > Interim Histology Manager > Mission Hospital System > Asheville, NC > > ------------------------------ > > Message: 9 > Date: Wed, 05 Sep 2007 16:00:47 -0400 > From: Phil McArdle > Subject: Re: [Histonet] Microwaves VENDOR RESPONSE > To: Joe Nocito > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <46DF0AEF.7030203@ebsciences.com> > Content-Type: text/plain; charset=UTF-8; format=flowed > > Hi Joe: > > Obviously, a microwave vendor hates to hear microwave horror stories, so > again, no argument - even though I'm not privy to details of the fried > biopsies in question or what type/vintage of microwave, anyone who's > experienced a malfunction involving patient samples doesn't want a > repeat performance. And pathology is, must be, risk averse. > > That said, again, any mechanical or electronic equipment can fail, or > user error can contribute; just look at the "hang-up" problems with > older tissue processors that are now ancient history. It's up to > manufacturers to minimize the possibilities of failure, since patient > care is at stake. Improvement is therefore a continual, ongoing process. > For example, while for years EBS microwave processors incorporated > safety shutdown modes in the event of vent failure, probe failure (open > and closed) and many other component-related issues, about two years ago > we determined that the microwave did not have a comprehensive set of > safeguards to deal with user errors, for example, temperature overshoots > caused by too small a container for a given power setting, or failure to > place the temperature probe in solution. So we developed multiple safety > mechanisms to head off user errors of this sort. > > PMM > -- > Phil McArdle > Microwave Product Manager > > Energy Beam Sciences, Inc. > 29-B Kripes Rd. > East Granby, CT 06026 > > Tel: 800.992.9037 x 341 > Mobile: 860.597.6796 > Fax: 860.653.0422 > > pmcardle@ebsciences.com > www.ebsciences.com > > Joe Nocito wrote: > > are you sure it's the willies and not the johnnies? > > Since the magnetron or whatever it was that fried my tissue, I'd wait > > for the traditional processing. Call me a dinosaur, but I really don't > > like doing special stains in the microwave. The only thing I use a > > microwave for at my house is to defrost and reheat stuff (technical > > term). I'm sure there are people out there who can cook a 6 course > > gourmet meal. My best friend can process all types of tissue from > > biopsies to uterus. > > As a matter of fact, he was there grossing when something went wrong > > and told me that he's never seen tissue like that before. > > > > JTT > > ----- Original Message ----- From: "Phil McArdle" > > > > To: "Joe Nocito" > > Cc: > > Sent: Wednesday, September 05, 2007 11:16 AM > > Subject: Re: [Histonet] Microwaves VENDOR RESPONSE > > > > > >> Hi Joe: > >> > >> No argument there. I'm painfully aware of both a mindset of "a > >> microwave 'should' cost less than $100," and of a dearth of funding > >> for pathology in general (popular shows like CSI to the contrary). :-) > >> I'd still suggest that $1749 is a heck of a lot better (and a lot less > >> laughable) than the $18,000 or $30,000 that's widely quoted and > >> posted, and it's the exact reason we brought an under-$2000 lab > >> microwave to market in the first place. > >> > >> One could argue just as convincingly against all kinds of specialized > >> equipment or reagents on the basis of cost, not just microwaves. We > >> all know of everything from saliva to cheap rice steamers being used > >> in histo labs, and while they may actually be perfectly serviceable, > >> from the standpoint of repeatability or liability, this kind of thing > >> gives me the willies (and that's a technical term). My yardstick is > >> always "what would I be comfortable with if my kid's diagnosis hung in > >> the balance?" > >> > >> Healthy debate is good! > >> > >> Phil > >> -- > >> Phil McArdle > >> Microwave Product Manager > >> > >> Energy Beam Sciences, Inc. > >> 29-B Kripes Rd. > >> East Granby, CT 06026 > >> > >> Tel: 800.992.9037 x 341 > >> Mobile: 860.597.6796 > >> Fax: 860.653.0422 > >> > >> pmcardle@ebsciences.com > >> www.ebsciences.com > >> > >> > >> > >> > >> > >> > >> Joe Nocito wrote: > >>> ok, but with the budgets today, many people can't afford a $1749 > >>> microwave when they can buy one at Walmart, K-Mart, or somewhere else > >>> for $79. > >>> Not to make you angry or anything, but I'm wondering how long has > >>> it been since you worked in a lab? Histo's budget is the first one > >>> cut in the lab because we are not essential. > >>> I can't count how many times I fought and fought for my budgets. > >>> If I tried to justify a $1749 microwave for special stains, HIER > >>> or whatever, I would have been laughed out the manager's office. > >>> Just my 4 cents. > >>> > >>> JTT > >>> ----- Original Message ----- From: "Phil McArdle" > >>> > >>> To: "Kathleen Boozer" > >>> Cc: > >>> Sent: Wednesday, September 05, 2007 9:07 AM > >>> Subject: Re: [Histonet] Microwaves VENDOR RESPONSE > >>> > >>> > >>>> Again, a microwave vendor weighs in (so far I haven't received any > >>>> flames), so read at your own risk. :-) > >>>> > >>>> At the risk of sounding overly and overtly commercial, after reading > >>>> post after post of $30,000+ and $18,000 and similarly high figures > >>>> for lab microwaves, I really feel the need to set the record > >>>> straight. Depending on the usage requirements, we have laboratory > >>>> microwaves as low as $1749 for a "bare bones" model for simple > >>>> operations, to mid-priced units, to under $11,000 for a vacuum > >>>> equipped microwave processor capable of the +/- 0.5 degree C > >>>> temperature control necessary for tissue processing. > >>>> > >>>> (I can feel the heat already!) > >>>> > >>>> There are many compelling reasons to replace a kitchen microwave > >>>> with a lab model; feel free to download, read, and even share with > >>>> colleagues our Microwave Companion at > >>>> > >>>> http://www.ebsciences.com/pdf/EBS_MW_COMPANION.pdf > >>>> > >>>> Best regards, and see you at NSH, > >>>> > >>>> Phil McArdle > >>>> > >>>> -- > >>>> Phil McArdle > >>>> Microwave Product Manager > >>>> > >>>> Energy Beam Sciences, Inc. > >>>> 29-B Kripes Rd. > >>>> East Granby, CT 06026 > >>>> > >>>> Tel: 800.992.9037 x 341 > >>>> Mobile: 860.597.6796 > >>>> Fax: 860.653.0422 > >>>> > >>>> pmcardle@ebsciences.com > >>>> www.ebsciences.com > >>>> > >>>> Kathleen Boozer wrote: > >>>>> What is the best microwave for a small lab using it only for > >>>>> heating Bouin's and Silver Nitrate for special stains? I just > >>>>> can't believe I would have to spend $30,000+ or slow down and use a > >>>>> waterbath. > >>>>> > >>>>> > >>>>> _______________________________________________ > >>>>> Histonet mailing list > >>>>> Histonet@lists.utsouthwestern.edu > >>>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >>>> > >>>> > >>>> > >>>> > >>>> I skate to where the puck is going to be, not to where it's been. > >>>> - Wayne Gretsky > >>>> > >>>> You must be the change you want to see in the world. > >>>> - Mahatma Gandhi > >>>> > >>>> NOTE: This message, together with any attachments, is intended only > >>>> for the use of the individual or entity to which it is addressed and > >>>> may contain information that is legally privileged, confidential and > >>>> exempt from disclosure. If you are not the intended recipient, > >>>> however, there's not a lot I can do about it, and it was probably my > >>>> mistake anyway. So please do the right thing and make this e-mail go > >>>> away. Thank you. > >>>> > >>>> _______________________________________________ > >>>> Histonet mailing list > >>>> Histonet@lists.utsouthwestern.edu > >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >>> > >>> > >>> _______________________________________________ > >>> Histonet mailing list > >>> Histonet@lists.utsouthwestern.edu > >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > >> > >> -- > >> Phil McArdle > >> Microwave Product Manager > >> > >> Energy Beam Sciences, Inc. > >> 29-B Kripes Rd. > >> East Granby, CT 06026 > >> > >> Tel: 800.992.9037 x 341 > >> Mobile: 860.597.6796 > >> Fax: 860.653.0422 > >> > >> pmcardle@ebsciences.com > >> www.ebsciences.com > >> > >> I skate to where the puck is going to be, not to where it's been. > >> - Wayne Gretsky > >> > >> You must be the change you want to see in the world. > >> - Mahatma Gandhi > >> > >> NOTE: This message, together with any attachments, is intended only > >> for the use of the individual or entity to which it is addressed and > >> may contain information that is legally privileged, confidential and > >> exempt from disclosure. If you are not the intended recipient, > >> however, there's not a lot I can do about it, and it was probably my > >> mistake anyway. So please do the right thing and make this e-mail go > >> away. Thank you. > > > > I skate to where the puck is going to be, not to where it's been. > - Wayne Gretsky > > You must be the change you want to see in the world. > - Mahatma Gandhi > > NOTE: This message, together with any attachments, is intended only for > the use of the individual or entity to which it is addressed and may > contain information that is legally privileged, confidential and exempt > from disclosure. If you are not the intended recipient, however, there's > not a lot I can do about it, and it was probably my mistake anyway. So > please do the right thing and make this e-mail go away. Thank you. > > ------------------------------ > > Message: 10 > Date: Wed, 5 Sep 2007 13:01:21 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Xylene > To: themagoos@rushmore.com, histonet@lists.utsouthwestern.edu > Message-ID: <329330.36844.qm@web61219.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Tissues usually become brittle, which difficulties sectioning. > Ren? J. > > themagoos wrote: > Can somebody tell me if there are any effects on tissue if > there is prolonged processing time in xylene? > > Jason McGough HT(ASCP) > Clinical Laboratory of the Black Hills > Account Representative - Anatomic Pathology > 2805 5th Street > Rapid City, SD 57701 > 605-343-2267 > jmcgough@clinlab.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > --------------------------------- > Boardwalk for $500? In 2007? Ha! > Play Monopoly Here and Now (it's updated for today's economy) at Yahoo! > Games. > > ------------------------------ > > Message: 11 > Date: Wed, 5 Sep 2007 14:09:02 -0600 > From: "Liz Chlipala" > Subject: RE: [Histonet] IHC anti-cleaved caspase 3 > To: "Cheryl Cross" , "Thomas Pier" > > Cc: carl.hobbs@kcl.ac.uk, histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset="windows-1250" > > I like the cell signaling antibody also, I tried biocare's but did not > have > much success with it. The cell signaling antibody also works with pronase > digestion, as well as the EDTA pH9 HIER. We have even used it on bone > sections with the pronase digestion. Works in multiple species, I have > used it > on human, rat, mouse, guinea pig, porcine and canine. Our protocol is > similar > to Cheryl's. It’s a bit pricy as antibodies go, but I feel its > worth it. > Good lot to lot consistency. We have probably gone through about 5 > different > lots, with not changing the protocol at all. I have a written protocol if > anyone is interested. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Manager > Premier Laboratory, LLC > P.O. Box 18592 > Boulder, CO 80308 > phone (303) 735-5001 > fax (303) 735-3540 > liz@premierlab.com > www.premierlab.com > > Ship to Address: > > Premier Laboratory, LLC > University of Colorado at Boulder > MCDB, Room A3B40 > Boulder, CO 80309 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Cross Sent: > Wednesday, > September 05, 2007 2:01 PM To: Thomas Pier Cc: carl.hobbs@kcl.ac.uk; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] IHC anti-cleaved caspase 3 > > I will second the Cell Signaling antibody - we have tried antibodies from > Promega (which gave tons of background staining)...then via this site i > was > pointed to Cell Signaling's monoclonal which we tried; the polyclonal > actually > has been working very well with minimal background (we were getting lots > of > respiratory epithelium and endothelium lighting up in control animals). I > have > been using it in mice with this protocol: > > EDTA with PASCAL > primary antibody 60 minutes, dilution 1:125 Rabbit envision DAB plus > > Hope this helps! > > Cheryl Cross, DVM, Dipl. ACVP > Researcher > University Corporation for Atmospheric Research College of Veterinary > Medicine > University of Tennessee Department of Pathology 2407 River Drive, Room > A201 > Knoxville, TN 37996-4542 > (423) 967-2724 fax: 865-974-5616 ccross@ucar.edu > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.5.485 / Virus Database: 269.13.5/990 - Release Date: 9/4/2007 > 10:36 > PM > > No virus found in this outgoing message. > Checked by AVG Free Edition. > Version: 7.5.485 / Virus Database: 269.13.5/990 - Release Date: 9/4/2007 > 10:36 > PM > > ------------------------------ > > Message: 12 > Date: Wed, 05 Sep 2007 16:13:36 -0400 > From: Sarah Clatterbuck Soper > Subject: [Histonet] Freezing mouse testes for frozen sections > To: histonet@lists.utsouthwestern.edu > Message-ID: <46DF0DF0.2030306@ciwemb.edu> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Hi all, > > I've started attempting to section unfixed frozen mouse testes in order > to placate a specific antibody we have to use. I am new to frozen > sections and I'm having trouble with the testes cracking when I freeze > them. I've tried both freezing in isopentane cooled on liquid nitrogen > and an acetone/dry ice slurry. Either way the testes crack, usually one > big crack end to end. Doesn't seem to be as much of a problem with our > mutant testes, which are about 1/3 the size of wild-type. I wish I > could just trim the wild-type down to a smaller size, but obviously > that's not an option! > > Any recommendations? > > Thanks so much! > > Sarah > > ------------------------------ > > Message: 13 > Date: Wed, 5 Sep 2007 15:49:48 -0500 > From: "Mike Pence" > Subject: [Histonet] Olympus BX40 > To: > Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C701@IS-E2K3.grhs.net> > Content-Type: text/plain; charset="us-ascii" > > Need some help, > > I am looking for a 60x objective for an Olympus microscope BX40. > Would anyone know where I might get a used one or if they even make one > this size for this scope? > > Thanks, > Mike > > ------------------------------ > > Message: 14 > Date: Wed, 5 Sep 2007 16:58:12 -0400 > From: "Yu, Jian" > Subject: [Histonet] Look for a used stereoscope > To: > Message-ID: > <7E0A77BFEB9A1E47A63F978E7116821F07986D6D@1upmc-msx11.acct.upmchs.net> > Content-Type: text/plain; charset="us-ascii" > > Does anyone know a good place to get a used stereoscope? I plan to use > it to examine intestinal tumors in mice. > > Thanks a lot for your information. > > ******************************************************************* > Jian Yu, Ph.D. > University of Pittsburgh Cancer Institute > Hillman Cancer Center Research Pavilion > Office Suite 2.26h > 5117 Centre Avenue, Pittsburgh, PA 15213 > ******************************************************************* > > ------------------------------ > > Message: 15 > Date: Thu, 6 Sep 2007 09:44:45 +1000 > From: "Tony Henwood" > Subject: RE: [Histonet] RDO decal > To: "Rene J Buesa" , "RENEE FISHER" > , > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > There is also the issue of EDTA, which is common in many RDO formulations. > Checking one MSDS for EDTA reveals: > > Ecological Information > Environmental Fate: > When released into the soil, this material is expected to leach into > groundwater. When released into the soil, this material may biodegrade > to a > moderate extent. When released into the soil, this material is not > expected > to evaporate significantly. When released into water, this material is > not > expected to evaporate significantly. This material is not expected to > significantly bioaccumulate. When released into the air, this material is > expected to be readily degraded by photolysis. Environmental Toxicity: > This material is not expected to be toxic to aquatic life. The > LC50/96-hour > values for fish are over 100 mg/l. > > So neutralisation may not be required. > If anyone has info to the contrary please advise. > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) > Laboratory Manager & Senior Scientist > The Children's Hospital at Westmead, > Locked Bag 4001, Westmead, 2145, AUSTRALIA. > Tel: 612 9845 3306 > Fax: 612 9845 3318 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: > Thursday, 6 > September 2007 1:07 AM To: RENEE FISHER; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] RDO decal > > Ren?e: > I have not heard of neutralizing RDO, but it would make sense if you > want to > be "gentle on your sewer system" BUT prepare to a large emission of carbon > dioxide when attempting to neutralize it with baking soda. RDO + baking > soda > (or sodium bicarbonate) will produce water, salt with the acid in RDO > (probably sodium chloride or common salt), and carbon dioxide in > stoichiometrical amounts (1 CO2 per every 1 NaCl). Therefore that > neutralization has to take place in a fumes hood, and you will have to > decide > which is worst: delivering acid to the sewer system, or carbon dioxide > (the > Greenhouse gas per excellence) to the atmosphere. It will be "your call". > Ren? J. > > RENEE FISHER wrote: > Has anyone heard of neutralizing RDO with baking soda to P.H. 7.0. Our > Histo > lab had a hazardous waste assessment, and the consultant suggested we not > throw the RDO down the drain but that we either collect it for waste > removal > or neutralize it with baking soda to p.h. 7.0. I have not heard of doing > this > and do not know of any procedure for it, everyone, anyone's help will be > greatly appreciated. > > Thanks, > Renee' > > _______________________________________________________________________________________ > > This email may contain confidential protected health information and/or > proprietary information belonging to the sender that is legally privileged > under local, state, or federal law. This information is intended only for > the > use of the individual or individuals who have received this. The > authorized > recipient of this information is prohibited from disclosing this > information > to any other party unless required to do so by law. If you are not the > intended recipient, you are hereby notified that any disclosure, copying, > distribution, or action taken in reliance on the contents of this email > is > strictly prohibited. If you have received this email in error, please > notify > the sender immediately to arrange for the disposal of this information. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > --------------------------------- > Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: mail, > news, > photos & more. _______________________________________________ Histonet > mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************* > This email and any files transmitted with it are confidential and intended > solely for the use of the individual or entity to whom they are addressed. > If > you are not the intended recipient, please delete it and notify the > sender. > > Views expressed in this message and any attachments are those of the > individual sender, and are not necessarily the views of The Children's > Hospital at Westmead > > This note also confirms that this email message has been > virus scanned and although no computer viruses were detected, The > Childrens > Hospital at Westmead accepts no liability for any consequential damage > resulting from email containing computer viruses. ********************************************************************** > > ------------------------------ > > Message: 16 > Date: Thu, 6 Sep 2007 11:23:00 +0900 > From: ChoiUl Soo > Subject: [Histonet] PTH antibody reacted with dog tissue... > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="ks_c_5601-1987" > > Hi Histonetters, > > I am interested in anti PTH antibody reacted with dog tissue. > If anyone has successful experience with any PTH antibody with dog tissue, > please tell me one. > > I have searched through the internet, and results came back with abcam and > SantaCruz PTH antibodies predicted to react with them. But they are not > sure > on it, just predicted on the basis of sequence homology. > (abcam say 94% homology with human) I don't have good exprience with Santa > Cruz, and never used one by Abcam. Should I rely on it, or find another > one? > > Let me hear your experience. > > I would appreciate your advice or comment on this. > > Thank you~. > > Ul Soo Choi, DVM, PhDKRF priority zoonotic disease research institute, > College > of Veterinary Medicine, Seoul National University, Shilim9 dong, Gwanakgu, > Seoul, Korea 151-742Tel. 82-02-880-8688 Mobile. 82-016-9228-8634Fax. > 82-02-880- > 8662 > > _________________________________________________________________ > ???? ?????? ????, Windows Live Space! > http://www.spaces.live.com > > ------------------------------ > > Message: 17 > Date: Thu, 6 Sep 2007 01:57:04 -0400 > From: "Michelle McCoy" > Subject: [Histonet] Floater sources > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <25355ef80709052257i32794198gd5a64b94758cc1ea@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > I was reading some of the old archived messages on floaters, and being > somewhat new to the field of histotechnology was curious about how > floaters > can be attributed to a particular tech who performed work on the block. > For > example, couldn't other sources of contamination be from the automatic > stainer, processor carryover (cassette not completely closed/or if closed, > friable tissue through the slats eg if sponge not used on larger > specimen). > In some previous labs I've worked in- floaters were quickly attributed to > the cutter, embedder or grosser and might result in a "write up". If the > floater is seen in the block how can you differentiate if it came from the > grosser carryover/embedder carryover/processing carryover/unsigned > re-embedder/or even possibly even client carry over between patients or > different specimen types of the same patient. > And if it is not in the block --differentiating between the > cutter/automatic > stainer (I've seen specks of tissue debris in automatic stainers/ sections > falling off the slides and into the reagents etc). Obviously all should be > done to minimize the factors, but I'm just not clear how a single source > is > pinpointed and potentially blamed for the event (depending on the lab > policy). Thanks for any ideas on this. > > ------------------------------ > > Message: 18 > Date: Thu, 6 Sep 2007 08:07:38 +0100 > From: "Kemlo Rogerson" > Subject: RE: [Histonet] Xylene > To: , > Message-ID: > <86ADE4EB583CE64799A9924684A0FBBF0222EC6C@wahtntex2.waht.swest.nhs.uk> > Content-Type: text/plain; charset="us-ascii" > > Can somebody tell me if there are any effects on tissue if there is > prolonged processing time in xylene? > > Jason McGough HT(ASCP) > Clinical Laboratory of the Black Hills > Account Representative - Anatomic Pathology > 2805 5th Street > Rapid City, SD 57701 > 605-343-2267 > jmcgough@clinlab.com > > Classically it is said to harden, plus more lipids could be removed. > Personally I'm equivocal about that thought; if properly fixed prior to > processing I would have thought the hardening effects of xylene were > much less than that of the coagulant fixative ethanol. If you do have > hard tissue then there is a restorative fluid one can use which I think > has oil of cedarwood in it but I don't know the formula off hand. I know > it works cos when I was a pup I used it sometimes. > > Kemlo Rogerson > Pathology Manager > DD 01934 647057 or extension 3311 > Mob 07749 754194; Pager 07659 597107; > > Sunshine is delicious, rain is refreshing, wind braces us up, snow is > exhilarating; there is really no such thing as bad weather, only > different kinds of good weather. --John Ruskin > > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. > Please inform me that this message has gone astray before deleting it. > Thank you for your co-operation > > ------------------------------ > > Message: 19 > Date: Thu, 6 Sep 2007 08:17:36 +0100 > From: "Kemlo Rogerson" > Subject: RE: [Histonet] Floater sources > To: "Michelle McCoy" , > > Message-ID: > <86ADE4EB583CE64799A9924684A0FBBF0222EC6D@wahtntex2.waht.swest.nhs.uk> > Content-Type: text/plain; charset="us-ascii" > > I was reading some of the old archived messages on floaters, and being > somewhat new to the field of histotechnology was curious about how > floaters can be attributed to a particular tech who performed work on > the block. For example, couldn't other sources of contamination be from > the automatic stainer, processor carryover (cassette not completely > closed/or if closed, friable tissue through the slats eg if sponge not > used on larger specimen). In some previous labs I've worked in- floaters > were quickly attributed to the cutter, embedder or grosser and might > result in a "write up". If the floater is seen in the block how can you > differentiate if it came from the grosser carryover/embedder > carryover/processing carryover/unsigned re-embedder/or even possibly > even client carry over between patients or different specimen types of > the same patient. And if it is not in the block --differentiating > between the cutter/automatic stainer (I've seen specks of tissue debris > in automatic stainers/ sections falling off the slides and into the > reagents etc). Obviously all should be done to minimize the factors, but > I'm just not clear how a single source is pinpointed and potentially > blamed for the event (depending on the lab policy). Thanks for any ideas > on this. > > You are exactly correct, floaters can be attributed to a variety of > causes, processing machines that aren't regularly changed, transfer on > the cutting up forceps, on the waterbath and on the forceps of the > embedder. If the floater was from a block that was cut, embedded, cut up > before the section with the floater then the culprit is obvious. If the > section was cut by someone who didn't cut the block from which the > floater floated, then the culprit is the embedder, machine,or cutter up. > Realisticaly I would have thought that most floaters would be from the > embedder, cutter up, then sectioner as the latter has the greater > likelihood of seeing the error of his/ her ways. Waxy forceps of messy > forceps, in my experience are the usual culprit but in some instances, > necrotic tumours can shed cells in the processor and even the stainer. > > Floaters are usually obvious as they tend to be at a different level > than the tissue section and I'm afraid they are a fact of life. You can > reduce the incidence but sadly never eradicate them. > > Kemlo Rogerson > Pathology Manager > DD 01934 647057 or extension 3311 > Mob 07749 754194; Pager 07659 597107; > > Sunshine is delicious, rain is refreshing, wind braces us up, snow is > exhilarating; there is really no such thing as bad weather, only > different kinds of good weather. --John Ruskin > > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. > Please inform me that this message has gone astray before deleting it. > Thank you for your co-operation > > ------------------------------ > > Message: 20 > Date: Thu, 6 Sep 2007 04:18:04 -0400 > From: "Victoria Baker" > Subject: [Histonet] Fwd: Users of Lab Vision autostainer > To: histonet > Message-ID: > <4f016b690709060118t532d22c1if250116e0a6c9af1@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > ---------- Forwarded message ---------- > From: Victoria Baker > Date: Sep 5, 2007 9:02 AM > Subject: Users of Lab Vision autostainer > To: Histo Net list server > > Hi > > I'm a new user of the Lab Vision autostainer and I'm looking to see if > I can find users in Histo-land that have experience with it. The > facility only works with human tissue and all of the antibodies are > for dx purposes. > > My key questions are as follows: > How many antibodies is your lab running? > How many users do you allow? > How many people do you allow programming rights and at what level of > supervision are they? > > How many of these antibodies are from Lab Vision? > a) are they concentrates or pre-dilutes? > b) for HIER are you using their PT modules/procedures or > your own equipment (microwave, steamer, pressure cooker etc) and in > house designed protocols for retrieval? > c) for digestion do you only use their Pro-K or have you > designed your own in-house methods using other reagents for digestion? > d) do you put your controls on the same slide as the patient? > e) are your controls in-house or commercial? > f) do you have more than one stainer hooked up to one computer > system? > > What Version of software do you currently have on your system? > > Any feed back would be very helpful. > > Thanks in advance. > > Vikki Baker > Interim Histology Manager > Mission Hospital System > Asheville, NC > > ------------------------------ > > Message: 21 > Date: Thu, 6 Sep 2007 02:28:57 -0600 > From: "Joseph Kapler" > Subject: RE: [Histonet] Xylene > To: "Rene J Buesa" , , > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Tissues processed in Xylene (especially over long periods of time) the > tissue becomes very brittle. > > Hope that is the response you were looking for. > > Joseph "DarkWolfe" Kapler > I'm an outsider outside of everything > I'm an outsider outside of everything > I'm an outsider outside of everything > Everything you know. Everything you know > It disturbs me so > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J > Buesa > Sent: Wednesday, September 05, 2007 14:01 > To: themagoos@rushmore.com; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Xylene > > Tissues usually become brittle, which difficulties sectioning. > Ren? J. > > themagoos wrote: > Can somebody tell me if there are any effects on tissue if > there is prolonged processing time in xylene? > > Jason McGough HT(ASCP) > Clinical Laboratory of the Black Hills > Account Representative - Anatomic Pathology > 2805 5th Street > Rapid City, SD 57701 > 605-343-2267 > jmcgough@clinlab.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > --------------------------------- > Boardwalk for $500? In 2007? Ha! > Play Monopoly Here and Now (it's updated for today's economy) at Yahoo! > Games. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.5.485 / Virus Database: 269.13.5/988 - Release Date: 9/4/2007 > 09:14 > > No virus found in this outgoing message. > Checked by AVG Free Edition. > Version: 7.5.485 / Virus Database: 269.13.5/988 - Release Date: 9/4/2007 > 09:14 > > ------------------------------ > > Message: 22 > Date: Thu, 6 Sep 2007 09:59:13 +0100 > From: Malam Jacqueline > Subject: [Histonet] Unsubscribe > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain > > Please would you unsubscribe me as I am retiring tomorrow - thanks > > Jacqui malam > Lancaster > uk > > DISCLAIMER: This e-mail is confidential and privileged. If you are not the > intended recipient please accept our apologies; please do not disclose, > copy > or distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. Please > inform postmaster@rli.mbht.nhs.uk that this message has gone astray before > deleting it. Comments or opinions expressed in this email are those of > their respective contributors only. The views expressed do not represent > the > views of the Trust, its management or employees. University Hospitals of > Morecambe Bay NHS Trust is not responsible and disclaims any and all > liability for the content of comments written within.Thank you for your > co-operation. > > ------------------------------ > > Message: 23 > Date: Thu, 6 Sep 2007 06:31:58 -0500 > From: "Joe Nocito" > Subject: Re: [Histonet] Floater sources > To: "Michelle McCoy" , > > Message-ID: <004201c7f079$8f5aa560$0202a8c0@yourxhtr8hvc4p> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > Michelle, > first. welcome to world of histology. > Let's begin at the grossing table- all grossers are trained to wipe off > the > table after each case- but that doesn't mean floaters can't happen, but > the > incidence is low > third- embedders should have been taught to clean the embedding area after > each case and at the end of the day, but this is a good source for > contamination. You can see this is the block. > fourth- the cutters should wipe off their waterbaths after each block. > This > probably is the most likely source of contamination. I've inspected some > labs where one waterbath was just covered with previous ribbons, attached > to > the side and floating on the waterbath. > fifth- in my experience, it is highly unlikely that floaters came from the > stainer just by the shear movement of the slides and water. > > The bottom line is that everyone needs to be neat and clean and be > meticulous. Remember, cleanliness is next to Godliness. > I had a testicular seminoma where no matter what we did, floaters still > were > on cervical bxs and endometrial bxs. The medical and I decided that we > would handle cases like these separately. They were processed, embedded, > cut > and stained separately. Once the case was completed, we changed all the > solutions on the tissue processor and stainer. Who ever cut the blocks had > to dismantle their knife holder and microtome to clean it thoroughly. A > pain > in the butt, but was a necessary evil. > > I hope this helped a little. Good luck. > > Joe The Toe > ----- Original Message ----- > From: "Michelle McCoy" > To: > Sent: Thursday, September 06, 2007 12:57 AM > Subject: [Histonet] Floater sources > > >I was reading some of the old archived messages on floaters, and being > > somewhat new to the field of histotechnology was curious about how > > floaters > > can be attributed to a particular tech who performed work on the block. > > For > > example, couldn't other sources of contamination be from the automatic > > stainer, processor carryover (cassette not completely closed/or if > > closed, > > friable tissue through the slats eg if sponge not used on larger > > specimen). > > In some previous labs I've worked in- floaters were quickly attributed > > to > > the cutter, embedder or grosser and might result in a "write up". If the > > floater is seen in the block how can you differentiate if it came from > > the > > grosser carryover/embedder carryover/processing carryover/unsigned > > re-embedder/or even possibly even client carry over between patients or > > different specimen types of the same patient. > > And if it is not in the block --differentiating between the > > cutter/automatic > > stainer (I've seen specks of tissue debris in automatic stainers/ > > sections > > falling off the slides and into the reagents etc). Obviously all should > > be > > done to minimize the factors, but I'm just not clear how a single source > > is > > pinpointed and potentially blamed for the event (depending on the lab > > policy). Thanks for any ideas on this. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 46, Issue 6 > *************************************** ------- End of Original Message ------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dbpiontek <@t> hotmail.com Thu Sep 6 15:46:44 2007 From: dbpiontek <@t> hotmail.com (Denise Piontek) Date: Thu Sep 6 15:47:00 2007 Subject: [Histonet] Dimedone PAS Message-ID: Is anyone out there familiar with this staining method to isolate glycogen? How about alternate fixation methods associated with the stain? Advice and references are greatly appreciated, Denise Bland-Piontek, HTL(ACSP)CTBS(AATB) NIBRI _________________________________________________________________ News, entertainment and everything you care about at Live.com. Get it now! http://www.live.com/getstarted.aspx From RSRICHMOND <@t> aol.com Thu Sep 6 16:01:58 2007 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Thu Sep 6 16:02:09 2007 Subject: [Histonet] Re: damaged cartilage Message-ID: About staining of damaged cartilage: A number of years ago I researched and wrote up a procedure for a trichrome stain suitable for staining normal and abnormal cartilage. I have never actually been able to try it, but a researcher I gave it to said that it worked satisfactorily. Bob Richmond Samurai Pathologist Knoxville TN ************************************** SAFRANIN O STAIN FOR CARTILAGE PURPOSE: This highly specialized trichrome technique has been used as a stain for normal and abnormal cartilage in osteoarthritis. SPECIMEN: Tissue fixed in neutral buffered formalin, routinely decalcified if necessary. Lillie used acidified formalin fixatives, with perhaps better results. PROCEDURE: 1. Hydrate paraffin sections to water. 2. Stain 6 minutes in WEIGERT'S HEMATOXYLIN. 3. Wash well in water. 4. Blue in dilute ammonia or other bluing reagent. 5. Stain 3 minutes in FAST GREEN. 6. Wash in 1% ACETIC ACID. 7. Stain 12 to 15 minutes in SAFRANIN O. 8. Drain sections, but do not wash in water. 9. Dehydrate rapidly (about ten dips in each bath) through two changes of 95% alcohol, two changes of absolute alcohol, and xylenes. The alcohols should be fresh. Xylene substitutes should be evaluated with caution. 10. Mount in resin (such as Permount). Results: nuclei are black, cytoplasm gray-green. Proteoglycans in normal cartilage stain uniformly red, while subchondral bone is blue-green. Metachromatically staining substances such as mucins and mast cell granules are orange-red. REAGENTS: WEIGERT'S HEMATOXYLIN: may be prepared or purchased. The shelf life of the completely mixed iron hematoxylin solution is short, no more than a few weeks. It might be possible to substitute an ordinary alum hematoxylin here, if nuclear detail were of no great concern. FAST GREEN: Dissolve 40 mg of fast green FCF (C.I. No. 42053) in 200 mL of distilled or deionized water. Add a crystal of thymol or a drop of phenol as a preservative. Expect a shelf life of a few months at room temperature. SAFRANIN: Dissolve 200 mg of safranin O (C.I. No. 50240) in 200 mL of distilled or deionized water. Add a crystal of thymol or a drop of phenol as a preservative. Expect a shelf life of a few months at room temperature. ACETIC ACID: Add 1 mL of glacial acetic acid to 100 mL of distilled or deionized water, or put about 8 drops in a 40 mL Coplin jar full of water. Prepare fresh every day of use, unless the stain is done very frequently. QUALITY ASSURANCE: Use dyes obtained from documented sources. Examine the slide for adequacy of staining. Use decalcified normal cartilage and bone as a control. SAFETY AND DISPOSAL: These reagents offer no special safety or disposal problems. They may be disposed of down the drain, with plenty of water. REFERENCES: 1. Lillie RD. Histologic technic and practical histochemistry, 3rd. ed., 1965, p. 507. Blakiston/McGraw-Hill, New York. The basic procedure, developed by Lillie himself, is given here. 2. Rosenberg L. Chemical basis for the histological use of safranin O in the study of articular cartilage. J Bone Joint Surg 53-A (1):69-82, 1971. Another paper by Mankin et al. in the same journal, p. 523, gives a histologic grading scale. 3. Hamerman D. The biology of osteoarthritis. N Engl J Med 320:1322-30, May 18th, 1989. Color plates illustrate the desired results of the stain. 4. Hamerman D., Dept. of Medicine, Montefiore Medical Center, Bronx NY, Oct. 5, 1989. More details of the method. 5. Conn HJ, Lillie RD. Biological Stains, 9th ed, pp 385-7. Waverly Press, Baltimore 1976. About safranin. 6. Prophet EB et al., eds. AFIP Laboratory Methods in Histology, p. 167. American Registry of Pathology, Washington DC 1992. Very short procedure. AUTHOR: Procedure written by Robert S. Richmond, M.D., F.C.A.P., Knoxville TN, October 1989. Bibliography updated June 1994. From mcauliff <@t> umdnj.edu Thu Sep 6 16:17:03 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Sep 6 16:17:32 2007 Subject: [Histonet] IHC staining weaker in... In-Reply-To: <20070906154448.M50194@mail.inonu.edu.tr> References: <20070906115124.F2E28EC022@inonu.edu.tr> <20070906154448.M50194@mail.inonu.edu.tr> Message-ID: <46E06E4F.4020609@umdnj.edu> Department of Pathology wrote: > Dear Participants, our diagnostic pathology lab has a problem. The paraffin sections > are stained weakly at the center but better at the periphery of the sections. What > could be the reason.??... thanks a lot.. Dr Nasuhi Engin Aydin, malatya Inonu > University Hospital, Turkey 44365. > > > ---------- Original Message ----------- > From: histonet-request@lists.utsouthwestern.edu > To: histonet@lists.utsouthwestern.edu > Sent: Thu, 6 Sep 2007 14:51:24 +0300 (EEST) > Subject: Histonet Digest, Vol 46, Issue 6 > > Incomplete removal of the paraffin could cause this. Geoff -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From Shirley.Chu <@t> moldev.com Thu Sep 6 16:49:45 2007 From: Shirley.Chu <@t> moldev.com (Chu, Shirley) Date: Thu Sep 6 16:50:22 2007 Subject: [Histonet] Laser Capture Microdissection Web Seminar Message-ID: An Arcturus LCM webinar will be presented on Thurs, Sept 12th by Dr. Joel Pachter of the University of Connecticut Health Center. The title of his talk is: "Validiation of LCM/QRT-PCR to Profile the Blook-Brain Barrier Transcriptome of the Cerebral Microvasculature In Situ" For futher information and/or to register for this webinar, please see the following website: http://www.moleculardevices.com/pages/webinar_arcturus.html Shirley Chu Applications Scientist Molecular Devices, now a part of MDS Analytical Technolgies From pereirafamily <@t> cox.net Thu Sep 6 19:21:03 2007 From: pereirafamily <@t> cox.net (pereirafamily) Date: Thu Sep 6 19:21:23 2007 Subject: [Histonet] slide labels Message-ID: <000b01c7f0e4$f9becfb0$e5600744@pereirafarm> Hey all, Is anyone experienced with using slide labels that supposedly don't come off with any chemicals? Do you also write on the slide and then put the sticker on? Our facility is transferring to these new labels from Shamrock and a few of us have some concerns. Could you please e-mail Marilyn Weiss at Marilyn.a.weiss@kp.org. Thanks. From lpwenk <@t> sbcglobal.net Thu Sep 6 19:32:38 2007 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Sep 6 19:32:53 2007 Subject: [Histonet] IHC staining weaker in... In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E273CD9@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <000601c7f0e6$97fc7f00$0202a8c0@HPPav2> Another variation on the theme - Underfixed tissue, so only the outside is fixed with the fixative, and the center is unfixed. Then the center is "fixed" with the alcohol on the tissue processor. So there are two different fixations going on. Most stains are designed for the charges on the tissue caused by formalin fixation. If tissue is primarily alcohol fixed, then the charges on the tissue are not the same as formalin fixed tissue, so the staining is different. Either make the tissue thinner (2-3 mm) and/or increase the time in the fixative. Either way, get the fixative into the center of the tissue AND allow it time to cross-link, BEFORE the tissue goes into the alcohol step. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Thursday, September 06, 2007 12:34 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC staining weaker in... What is your standard fixative? Have you made any changes in routine fixation recently? This sounds like a fixation problem. As a core research facility, we receive all kinds of tissues from various researchers, often in a variety of fixatives. I have noticed the artifact you describe in some tissues fixed in certain slow-penetrating fixatives. Some aggressive fixatives give very nice preservation in small tissue samples, but when larger tissues are immersed in such fixatives, the rapid fixation of the outer layer of the tissue actually sets up a barrier to penetration of the fixative into the deeper levels. The result is a poorly fixed interior with a very well fixed exterior, and the poorly fixed deeper areas often stain poorly. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Thu Sep 6 19:42:44 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu Sep 6 19:42:57 2007 Subject: [Histonet] Counterstain for GFP tagged Listeria In-Reply-To: References: Message-ID: Which company do you buy your fluorescent antibody from? We've never gotten GFP to work on paraffin sections. Emily -- Remember our war hysteria, when we called sauerkraut 'Liberty cabbage' and somebody actually proposed calling German measles, 'Liberty measles?'...Remember when the hick legislators in certain states, in obedience to William Jennings Bryan, who learned his biology from his pious old grandma, set up shop as scientific experts and made the whole world laugh itself sick by forbidding the teaching of evolution? --Sinclair Lewis, It Can't Happen Here, 1935 From cabramley <@t> students.latrobe.edu.au Thu Sep 6 23:01:39 2007 From: cabramley <@t> students.latrobe.edu.au (CLAIRE ANN KENTLER) Date: Thu Sep 6 23:01:56 2007 Subject: [Histonet] OCT Compound Message-ID: <91DC98B386D8614487170C699BE9299F04FE6107@stexchange1.students.ltu.edu.au> Hi all, I have been using OCT Compound to prepare frozen sections of calf Peyer's Patches and lymph nodes. I've recently run out of compound and due to some major university re-structure, it doesn't look like I'll be able to get hold of any before we need to take the next set of samples. My supervisor and I are madly searching for any information on the possibility of freezing sections without OCT. Does anyone have experience with this, or any ideas? Thanks! Claire ************************************************************************ *************************************** Claire Kentler Bachelor of Animal Science (Hons) Postgraduate Student Department of Agricultural Sciences La Trobe University Phone: 9479 1048 E-mail: cabramley@students.latrobe.edu.au From kappeler <@t> patho.unibe.ch Thu Sep 6 23:29:23 2007 From: kappeler <@t> patho.unibe.ch (Andi Kappeler) Date: Thu Sep 6 23:29:59 2007 Subject: [Histonet] MMP9 References: <006501c7f0a3$552a00a0$6401a8c0@Patsy> Message-ID: <00d101c7f107$ab151860$27955c82@patho.unibe.ch> Hi Patsy no idea, which clone Abcam is selling. We had reasonably good results (on human FFPE tissue) with clone 56-2A4, originally from Oncogene, now from Calbiochem (http://www.merckbiosciences.co.uk/Products/pds.asp?catno=IM37). We used it at 1 ug Ig/ml after HIER in microwave with a somewhat exotic retrieval buffer (100 mM Tris, 5% urea, pH 9.5). We had tried a different clone before (15W2) without success. Hope this helps. Andi Kappeler Institute of Pathology, University of Bern, Switzerland ----- Original Message ----- From: "Patsy Ruegg" To: Sent: Thursday, September 06, 2007 6:31 PM Subject: [Histonet] MMP9 > Does anyone have experience with MMP9 ms monoclonal antibody from Abcam on > ffpe tissue they could possibly share with me. This one IHC is driving me > crazy. > > Thank you, > > Patsy > > > > Patsy Ruegg, HT(ASCP)QIHC > > IHCtech, LLC > > 12635 Montview Blvd. Ste.215 > > Aurora, Colorado 80045 > > Phone: 720-859-4060 > > Fax: 720-859-4110 > > pruegg@ihctech.net > > www.ihctech.net > > www.ihcrg.org > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From garth <@t> apollosci.co.za Fri Sep 7 00:49:04 2007 From: garth <@t> apollosci.co.za (Garth Jerome) Date: Fri Sep 7 00:49:47 2007 Subject: [Histonet] IHC staining weaker in... In-Reply-To: <20070906154448.M50194@mail.inonu.edu.tr> Message-ID: <000001c7f112$cf0c65b0$7700a8c0@jhb.apollosci.co.za> Hello It sounds to me like inadequate fixation of the tissue. The fact that peripheral staining is good and gets worse towards the middle could indicate poor penetration of fixative. What is your tissue processing programme? What fixative are you using? Regards Garth Jerome Histologist Apollo Scientific cc Telephone : 27-11-466 7666 Facsimile : 27-11-466 7672 Cell phone : 084 504 1101 Email : garth@apollosci.co.za -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Department of Pathology Sent: 06 September 2007 05:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC staining weaker in... Dear Participants, our diagnostic pathology lab has a problem. The paraffin sections are stained weakly at the center but better at the periphery of the sections. What could be the reason.??... thanks a lot.. Dr Nasuhi Engin Aydin, malatya Inonu University Hospital, Turkey 44365. ---------- Original Message ----------- From: histonet-request@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Thu, 6 Sep 2007 14:51:24 +0300 (EEST) Subject: Histonet Digest, Vol 46, Issue 6 > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > Today's Topics: > > 1. Xylene (themagoos) > 2. RE:The great apoptosis stains debate (TUNEL, Caspases, etc) > (Melissa Gonzalez) > 3. Re: RDO decalcifier (Robert Richmond) > 4. Re: IHC anti-cleaved caspase 3 (Carl Hobbs) > 5. avian sperm cell topography (melissah rowe) > 6. Re: IHC anti-cleaved caspase 3 (Thomas Pier) > 7. Re: IHC anti-cleaved caspase 3 (Cheryl Cross) > 8. Fwd: Users of Lab Vision autostainer (Victoria Baker) > 9. Re: Microwaves VENDOR RESPONSE (Phil McArdle) > 10. Re: Xylene (Rene J Buesa) > 11. RE: IHC anti-cleaved caspase 3 (Liz Chlipala) > 12. Freezing mouse testes for frozen sections > (Sarah Clatterbuck Soper) > 13. Olympus BX40 (Mike Pence) > 14. Look for a used stereoscope (Yu, Jian) > 15. RE: RDO decal (Tony Henwood) > 16. PTH antibody reacted with dog tissue... (ChoiUl Soo) > 17. Floater sources (Michelle McCoy) > 18. RE: Xylene (Kemlo Rogerson) > 19. RE: Floater sources (Kemlo Rogerson) > 20. Fwd: Users of Lab Vision autostainer (Victoria Baker) > 21. RE: Xylene (Joseph Kapler) > 22. Unsubscribe (Malam Jacqueline) > 23. Re: Floater sources (Joe Nocito) > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 05 Sep 2007 10:21:51 -0700 > From: "themagoos" > Subject: [Histonet] Xylene > To: histonet@lists.utsouthwestern.edu > Message-ID: <46dee5af.14b.450a.1030817715@rushmore.com> > Content-Type: text/plain; charset="iso-8859-1" > > Can somebody tell me if there are any effects on tissue if > there is prolonged processing time in xylene? > > Jason McGough HT(ASCP) > Clinical Laboratory of the Black Hills > Account Representative - Anatomic Pathology > 2805 5th Street > Rapid City, SD 57701 > 605-343-2267 > jmcgough@clinlab.com > > ------------------------------ > > Message: 2 > Date: Wed, 5 Sep 2007 11:30:38 -0700 > From: "Melissa Gonzalez" > Subject: [Histonet] RE:The great apoptosis stains debate (TUNEL, > Caspases, etc) > To: > Message-ID: > <2884B897182A1D438C7BA24B9A8F94A20E701D@hqsvr01mail.cgi.com> > Content-Type: text/plain; charset="iso-8859-1" > > Hi all, > I have been around this mess a few years back, I gave up on TUNEL long ago for > the various reasons mentioned on the list recently. > > Per investigators requests, I have tried several ways to demonstrate cell > death, however methods such as PI or Annexin staining are more suitable for > whole cells, not tissue sections. I have also not had any luck staining for > Cytochrome C. > > We routinely use R&D Systems rabbit anti human/mouse active Caspase3 using a > high pH (EDTA) based Ag retrieval in a steamer (enzymes do not work). Works > like a charm. > > Any other suggestions? I know this topic has been thrown around a lot, but > these discussions after all, help us get our jobs done more proficiently. > > Melissa > > Melissa A. Gonz?lez Edick > R&D, Cell Genesys Inc. > 500 Forbes Blvd > South San Francisco, CA 94080 > p(650) 266-3168 > f (650) 266-3080 > > "It's not enough to believe what you see, you must also understand what you > see." -Leonardo Da Vinci > ------------------------------ > > Message: 6 > Date: Tue, 4 Sep 2007 15:18:39 -0400 > From: Cheryl Cross > Subject: Re: [Histonet] TUNEL, Formalin, DNA strand breaks > To: JR R > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <4C1E074D-064A-45D1-BD32-13D7E2222B26@aol.com> > Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed > > Hi all - > > I have been round and round with this issue myself; I was basically > told by people who've attempted it that TUNEL on FFPE sections is not > specific enough due to the formalin issue. > > If you are trying to nail apoptosis, what about anti-active > caspase-3? mind you, that staining can be a bit of a booger too, but > it should be more sensitive and specific for apoptosis (no references > to offer, i'm just repeating what i have been told). > > Cheryl Cross, DVM, Dipl. ACVP > Researcher > University Corporation for Atmospheric Research > College of Veterinary Medicine > University of Tennessee Department of Pathology > 2407 River Drive, Room A201 > Knoxville, TN 37996-4542 > (423) 967-2724 > fax: 865-974-5616 > ccross@ucar.edu > ------------------------------ > > Message: 5 > Hi Ray, > > Sheesh-- I read (and then lost) the paper maybe 5-7 years ago, back when I was > tearing my hair out over high background, or false positives for TUNEL stain > in formalin fixed, paraffin embedded arterial sections. I can troubleshoot > any immunostain, but the TUNEL and ISEL assays bedeviled me. > > The paper was titled something to the effect of "formaldehyde causes single > and double stranded DNA breaks," and was pretty emphatic. I'll look for the article. > > I think the assay could work in whole cells for flow, or maybe in frozen > tissue. My hunch at this point is that this is one of those rare cases where > lots and lots of peer reviewed articles are just plain wrong. > > Hey, if someone has a good protocol, I'd be willing to try it out, and I would > be delighted if I turn out to be mistaken. For now, I think TUNEL is the > assay of the beast. > > Oh, here is a thought--say you are looking at a 5 micron section through a > nucleus. The microtome blade pretty much had to create a lot of double > stranded DNA breaks, no? > > Jerry L. Ricks > Research Scientist > U.W. Medicine at South Lake Union > 815 Mercer Street > Seattle, WA 98109 > (206)-685-7190 > > From: koellingr@comcast.netTo: rosenfeldtek@hotmail.com; > histonet@lists.utsouthwestern.eduSubject: RE: [Histonet] TUNELDate: Fri, 31 > Aug 2007 23:17:03 +0000 > > Jerry, > Could you expand on or give references to formalin causing DNA strand breaks? > Double strand breaks, single strand, blunt end, overhanging? My pile of > papers and having done TUNEL for years says that formalin fixation is a very > good technique for TUNEL and many peer-reviewed articles in which TUNEL is > used as a technique, use formalin fixation and how can that be if formalin is > causing strand breaks? In fact one paper I'm looking at says that extended (5- > 7 weeks in formalin) fixation causes loss of TUNEL signal. If formalin is > causing breaks, you would assume that TUNEL pos signals would increase with > extended formalin fixation. Even the use of the monoclonal antibody F7-26, > for single stranded DNA, touts formalin fixation for their claims of > discriminating apoptosis from necrosis. > > The question asks about extended alcohol fixation but your answer is possibly > a lot of false positives because formalin causes DNA breaks. Does this imply > that alcohol won't? Have done a lot of TUNEL on alcohol fixed samples. True > I couldn't pretreat them and handle them they way I would handle FFPE tissue. > Also true that we could argue specificity and ability or not to discriminate > apoptosis from necrosis for quite a while. But if formalin itself is causing > the breaks in DNA, I and a lot of people are in big trouble with our science > projects and experiments. Also I can't envision why 2 cells are showing TUNEL > positivity while 2 of the same type of cells right next to them (and getting > the same formalin fix), are absolutely clean and there is no background? > > Thanks for any information you can provide. > > Ray Koelling > PhenoPath Laboratories > Seattle, WA > > ------------------------------ > > Message: 3 > Date: Wed, 5 Sep 2007 14:56:14 -0400 > From: "Robert Richmond" > Subject: [Histonet] Re: RDO decalcifier > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > I don't see how your hazmats people can tell you what to do to dispose > of RDO, since nobody has any idea what's in it. It's a murky yellow > liquid that continuously throws a black sediment that has to be > filtered out if you're to see your specimen in it. It's sort of the > ultimate secret formula, and I've always wondered why it's so popular > - I've had to use it many different places in my travels. > > I'd suggest either an ordinary proprietary decalcifier that's a clear > liquid, probably hydrochloric acid - or else save money and dilute > your own hydrochloric acid. You can re-use it for a while. When the > time comes to dispose of it, it can be safely diluted with a lot of > water and put down the drain. > > Remember - it's been stressed on this list many times - that "Decal" > is a brand name still in use, and that other brands of decalcifying > solution should not be called Decal. > > Bob Richmond > Samurai Pathologist (never a decalcified Carmelite) > Knoxville TN > > ------------------------------ > > Message: 4 > Date: Wed, 5 Sep 2007 20:29:36 +0100 > From: "Carl Hobbs" > Subject: Re: [Histonet] IHC anti-cleaved caspase 3 > To: "Histonet" > Message-ID: <001801c7eff3$18894850$4101a8c0@carlba65530bda> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > Hi. > > Recent Cleaved caspase 3 posts have not mentioned the antibody details: I > would be grateful for the source of these Abs. > Carl > > ------------------------------ > > Message: 5 > Date: Wed, 5 Sep 2007 14:36:50 -0500 > From: melissah rowe > Subject: [Histonet] avian sperm cell topography > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed > > Hi, > > I am a PhD student working on a behavioral ecology project on > Australian fairy-wrens. I am currently trying to find out if I can > determine the dimensions of discrete sections of individual sperm > cells, i.e. acrosome length, nucleus length, midpiece length and tail > length using the material I currently have available. I have prepared > slide smears of sperm that has been previously stained with an eosin- > nigrosin stain (to determine cell viability) and would like to be > able to somehow stain different segments of the sperm so that I can > identify and measure them using an ocular micrometer on a bright > field or phase contrast microscope set up. It is my understanding > that I cannot use fluorescent stains given that I have already used a > colormetric stain (the eosin-nigrosin). If anyone has suggestions for > ways to identify discrete regions of avian (passerine) sperm cells I > would be very grateful to hear from you. > > Many thanks in advance, > melissah > > ----- > melissah rowe > > PhD candidate > Department of Ecology & Evolution > University of Chicago > E. 57th Street, Chicago, IL, 60637 > ph: +1 773-702-3070 > fax: + 1 773-702-9740 > > email: melissah@uchicago.edu > > ------------------------------ > > Message: 6 > Date: Wed, 05 Sep 2007 14:39:04 -0500 > From: "Thomas Pier" > Subject: Re: [Histonet] IHC anti-cleaved caspase 3 > To: , > Message-ID: <46DEBF88020000DF0000A736@gwmail.medicine.wisc.edu> > Content-Type: text/plain; charset=US-ASCII > > Cell Signalling Technology has a good rabbit monoclonal for Cleaved Caspase-3. > > Tom Pier > > >>> "Carl Hobbs" 09/05/07 2:29 PM >>> > Hi. > > Recent Cleaved caspase 3 posts have not mentioned the antibody details: I > would be grateful for the source of these Abs. > Carl > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 7 > Date: Wed, 5 Sep 2007 15:54:35 -0400 > From: Cheryl Cross > Subject: Re: [Histonet] IHC anti-cleaved caspase 3 > To: "Thomas Pier" > Cc: carl.hobbs@kcl.ac.uk, histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed > > I will second the Cell Signaling antibody - we have tried antibodies > from Promega (which gave tons of background staining)...then via this > site i was pointed to Cell Signaling's monoclonal which we tried; the > polyclonal actually has been working very well with minimal > background (we were getting lots of respiratory epithelium and > endothelium lighting up in control animals). I have been using it in > mice with this protocol: > > EDTA with PASCAL > primary antibody 60 minutes, dilution 1:125 > Rabbit envision > DAB plus > > Hope this helps! > > Cheryl Cross, DVM, Dipl. ACVP > Researcher > University Corporation for Atmospheric Research > College of Veterinary Medicine > University of Tennessee Department of Pathology > 2407 River Drive, Room A201 > Knoxville, TN 37996-4542 > (423) 967-2724 > fax: 865-974-5616 > ccross@ucar.edu > > > > > ------------------------------ > > Message: 8 > Date: Wed, 5 Sep 2007 15:59:45 -0400 > From: "Victoria Baker" > Subject: [Histonet] Fwd: Users of Lab Vision autostainer > To: "Histo Net list server" > Message-ID: > <4f016b690709051259rcdffccet70d7f97ae0457707@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > One more try, first one didn't go through it seems! Thanks > > ---------- Forwarded message ---------- > From: Victoria Baker > Date: Sep 5, 2007 9:02 AM > Subject: Users of Lab Vision autostainer > To: Histo Net list server > > Hi > > I'm a new user of the Lab Vision autostainer and I'm looking to see if > I can find users in Histo-land that have experience with it. The > facility only works with human tissue and all of the antibodies are > for dx purposes. > > My key questions are as follows: > How many antibodies is your lab running? > How many users do you allow? > How many people do you allow programming rights and at what level of > supervision are they? > > How many of these antibodies are from Lab Vision? > a) are they concentrates or pre-dilutes? > b) for HIER are you using their PT modules/procedures or > your own equipment (microwave, steamer, pressure cooker etc) and in > house designed protocols for retrieval? > c) for digestion do you only use their Pro-K or have you > designed your own in-house methods using other reagents for digestion? > d) do you put your controls on the same slide as the patient? > e) are your controls in-house or commercial? > f) do you have more than one stainer hooked up to one computer system? > > What Version of software do you currently have on your system? > > Any feed back would be very helpful. > > Thanks in advance. > > Vikki Baker > Interim Histology Manager > Mission Hospital System > Asheville, NC > > ------------------------------ > > Message: 9 > Date: Wed, 05 Sep 2007 16:00:47 -0400 > From: Phil McArdle > Subject: Re: [Histonet] Microwaves VENDOR RESPONSE > To: Joe Nocito > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <46DF0AEF.7030203@ebsciences.com> > Content-Type: text/plain; charset=UTF-8; format=flowed > > Hi Joe: > > Obviously, a microwave vendor hates to hear microwave horror stories, so > again, no argument - even though I'm not privy to details of the fried > biopsies in question or what type/vintage of microwave, anyone who's > experienced a malfunction involving patient samples doesn't want a > repeat performance. And pathology is, must be, risk averse. > > That said, again, any mechanical or electronic equipment can fail, or > user error can contribute; just look at the "hang-up" problems with > older tissue processors that are now ancient history. It's up to > manufacturers to minimize the possibilities of failure, since patient > care is at stake. Improvement is therefore a continual, ongoing process. > For example, while for years EBS microwave processors incorporated > safety shutdown modes in the event of vent failure, probe failure (open > and closed) and many other component-related issues, about two years ago > we determined that the microwave did not have a comprehensive set of > safeguards to deal with user errors, for example, temperature overshoots > caused by too small a container for a given power setting, or failure to > place the temperature probe in solution. So we developed multiple safety > mechanisms to head off user errors of this sort. > > PMM > -- > Phil McArdle > Microwave Product Manager > > Energy Beam Sciences, Inc. > 29-B Kripes Rd. > East Granby, CT 06026 > > Tel: 800.992.9037 x 341 > Mobile: 860.597.6796 > Fax: 860.653.0422 > > pmcardle@ebsciences.com > www.ebsciences.com > > Joe Nocito wrote: > > are you sure it's the willies and not the johnnies? > > Since the magnetron or whatever it was that fried my tissue, I'd wait > > for the traditional processing. Call me a dinosaur, but I really don't > > like doing special stains in the microwave. The only thing I use a > > microwave for at my house is to defrost and reheat stuff (technical > > term). I'm sure there are people out there who can cook a 6 course > > gourmet meal. My best friend can process all types of tissue from > > biopsies to uterus. > > As a matter of fact, he was there grossing when something went wrong > > and told me that he's never seen tissue like that before. > > > > JTT > > ----- Original Message ----- From: "Phil McArdle" > > To: "Joe Nocito" > > Cc: > > Sent: Wednesday, September 05, 2007 11:16 AM > > Subject: Re: [Histonet] Microwaves VENDOR RESPONSE > > > > > >> Hi Joe: > >> > >> No argument there. I'm painfully aware of both a mindset of "a > >> microwave 'should' cost less than $100," and of a dearth of funding > >> for pathology in general (popular shows like CSI to the contrary). :-) > >> I'd still suggest that $1749 is a heck of a lot better (and a lot less > >> laughable) than the $18,000 or $30,000 that's widely quoted and > >> posted, and it's the exact reason we brought an under-$2000 lab > >> microwave to market in the first place. > >> > >> One could argue just as convincingly against all kinds of specialized > >> equipment or reagents on the basis of cost, not just microwaves. We > >> all know of everything from saliva to cheap rice steamers being used > >> in histo labs, and while they may actually be perfectly serviceable, > >> from the standpoint of repeatability or liability, this kind of thing > >> gives me the willies (and that's a technical term). My yardstick is > >> always "what would I be comfortable with if my kid's diagnosis hung in > >> the balance?" > >> > >> Healthy debate is good! > >> > >> Phil > >> -- > >> Phil McArdle > >> Microwave Product Manager > >> > >> Energy Beam Sciences, Inc. > >> 29-B Kripes Rd. > >> East Granby, CT 06026 > >> > >> Tel: 800.992.9037 x 341 > >> Mobile: 860.597.6796 > >> Fax: 860.653.0422 > >> > >> pmcardle@ebsciences.com > >> www.ebsciences.com > >> > >> > >> > >> > >> > >> > >> Joe Nocito wrote: > >>> ok, but with the budgets today, many people can't afford a $1749 > >>> microwave when they can buy one at Walmart, K-Mart, or somewhere else > >>> for $79. > >>> Not to make you angry or anything, but I'm wondering how long has > >>> it been since you worked in a lab? Histo's budget is the first one > >>> cut in the lab because we are not essential. > >>> I can't count how many times I fought and fought for my budgets. > >>> If I tried to justify a $1749 microwave for special stains, HIER > >>> or whatever, I would have been laughed out the manager's office. > >>> Just my 4 cents. > >>> > >>> JTT > >>> ----- Original Message ----- From: "Phil McArdle" > >>> > >>> To: "Kathleen Boozer" > >>> Cc: > >>> Sent: Wednesday, September 05, 2007 9:07 AM > >>> Subject: Re: [Histonet] Microwaves VENDOR RESPONSE > >>> > >>> > >>>> Again, a microwave vendor weighs in (so far I haven't received any > >>>> flames), so read at your own risk. :-) > >>>> > >>>> At the risk of sounding overly and overtly commercial, after reading > >>>> post after post of $30,000+ and $18,000 and similarly high figures > >>>> for lab microwaves, I really feel the need to set the record > >>>> straight. Depending on the usage requirements, we have laboratory > >>>> microwaves as low as $1749 for a "bare bones" model for simple > >>>> operations, to mid-priced units, to under $11,000 for a vacuum > >>>> equipped microwave processor capable of the +/- 0.5 degree C > >>>> temperature control necessary for tissue processing. > >>>> > >>>> (I can feel the heat already!) > >>>> > >>>> There are many compelling reasons to replace a kitchen microwave > >>>> with a lab model; feel free to download, read, and even share with > >>>> colleagues our Microwave Companion at > >>>> > >>>> http://www.ebsciences.com/pdf/EBS_MW_COMPANION.pdf > >>>> > >>>> Best regards, and see you at NSH, > >>>> > >>>> Phil McArdle > >>>> > >>>> -- > >>>> Phil McArdle > >>>> Microwave Product Manager > >>>> > >>>> Energy Beam Sciences, Inc. > >>>> 29-B Kripes Rd. > >>>> East Granby, CT 06026 > >>>> > >>>> Tel: 800.992.9037 x 341 > >>>> Mobile: 860.597.6796 > >>>> Fax: 860.653.0422 > >>>> > >>>> pmcardle@ebsciences.com > >>>> www.ebsciences.com > >>>> > >>>> Kathleen Boozer wrote: > >>>>> What is the best microwave for a small lab using it only for > >>>>> heating Bouin's and Silver Nitrate for special stains? I just > >>>>> can't believe I would have to spend $30,000+ or slow down and use a > >>>>> waterbath. > >>>>> > >>>>> > >>>>> _______________________________________________ > >>>>> Histonet mailing list > >>>>> Histonet@lists.utsouthwestern.edu > >>>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >>>> > >>>> > >>>> > >>>> > >>>> I skate to where the puck is going to be, not to where it's been. > >>>> - Wayne Gretsky > >>>> > >>>> You must be the change you want to see in the world. > >>>> - Mahatma Gandhi > >>>> > >>>> NOTE: This message, together with any attachments, is intended only > >>>> for the use of the individual or entity to which it is addressed and > >>>> may contain information that is legally privileged, confidential and > >>>> exempt from disclosure. If you are not the intended recipient, > >>>> however, there's not a lot I can do about it, and it was probably my > >>>> mistake anyway. So please do the right thing and make this e-mail go > >>>> away. Thank you. > >>>> > >>>> _______________________________________________ > >>>> Histonet mailing list > >>>> Histonet@lists.utsouthwestern.edu > >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >>> > >>> > >>> _______________________________________________ > >>> Histonet mailing list > >>> Histonet@lists.utsouthwestern.edu > >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > >> > >> -- > >> Phil McArdle > >> Microwave Product Manager > >> > >> Energy Beam Sciences, Inc. > >> 29-B Kripes Rd. > >> East Granby, CT 06026 > >> > >> Tel: 800.992.9037 x 341 > >> Mobile: 860.597.6796 > >> Fax: 860.653.0422 > >> > >> pmcardle@ebsciences.com > >> www.ebsciences.com > >> > >> I skate to where the puck is going to be, not to where it's been. > >> - Wayne Gretsky > >> > >> You must be the change you want to see in the world. > >> - Mahatma Gandhi > >> > >> NOTE: This message, together with any attachments, is intended only > >> for the use of the individual or entity to which it is addressed and > >> may contain information that is legally privileged, confidential and > >> exempt from disclosure. If you are not the intended recipient, > >> however, there's not a lot I can do about it, and it was probably my > >> mistake anyway. So please do the right thing and make this e-mail go > >> away. Thank you. > > > > I skate to where the puck is going to be, not to where it's been. > - Wayne Gretsky > > You must be the change you want to see in the world. > - Mahatma Gandhi > > NOTE: This message, together with any attachments, is intended only for > the use of the individual or entity to which it is addressed and may > contain information that is legally privileged, confidential and exempt > from disclosure. If you are not the intended recipient, however, there's > not a lot I can do about it, and it was probably my mistake anyway. So > please do the right thing and make this e-mail go away. Thank you. > > ------------------------------ > > Message: 10 > Date: Wed, 5 Sep 2007 13:01:21 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Xylene > To: themagoos@rushmore.com, histonet@lists.utsouthwestern.edu > Message-ID: <329330.36844.qm@web61219.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Tissues usually become brittle, which difficulties sectioning. > Ren? J. > > themagoos wrote: > Can somebody tell me if there are any effects on tissue if > there is prolonged processing time in xylene? > > Jason McGough HT(ASCP) > Clinical Laboratory of the Black Hills > Account Representative - Anatomic Pathology > 2805 5th Street > Rapid City, SD 57701 > 605-343-2267 > jmcgough@clinlab.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > --------------------------------- > Boardwalk for $500? In 2007? Ha! > Play Monopoly Here and Now (it's updated for today's economy) at Yahoo! Games. > > ------------------------------ > > Message: 11 > Date: Wed, 5 Sep 2007 14:09:02 -0600 > From: "Liz Chlipala" > Subject: RE: [Histonet] IHC anti-cleaved caspase 3 > To: "Cheryl Cross" , "Thomas Pier" > > Cc: carl.hobbs@kcl.ac.uk, histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset="windows-1250" > > I like the cell signaling antibody also, I tried biocare's but did not have > much success with it. The cell signaling antibody also works with pronase > digestion, as well as the EDTA pH9 HIER. We have even used it on bone > sections with the pronase digestion. Works in multiple species, I have used it > on human, rat, mouse, guinea pig, porcine and canine. Our protocol is similar > to Cheryl's. It’s a bit pricy as antibodies go, but I feel its worth it. > Good lot to lot consistency. We have probably gone through about 5 different > lots, with not changing the protocol at all. I have a written protocol if > anyone is interested. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Manager > Premier Laboratory, LLC > P.O. Box 18592 > Boulder, CO 80308 > phone (303) 735-5001 > fax (303) 735-3540 > liz@premierlab.com > www.premierlab.com > > Ship to Address: > > Premier Laboratory, LLC > University of Colorado at Boulder > MCDB, Room A3B40 > Boulder, CO 80309 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Cross Sent: Wednesday, > September 05, 2007 2:01 PM To: Thomas Pier Cc: carl.hobbs@kcl.ac.uk; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] IHC anti-cleaved caspase 3 > > I will second the Cell Signaling antibody - we have tried antibodies from > Promega (which gave tons of background staining)...then via this site i was > pointed to Cell Signaling's monoclonal which we tried; the polyclonal actually > has been working very well with minimal background (we were getting lots of > respiratory epithelium and endothelium lighting up in control animals). I have > been using it in mice with this protocol: > > EDTA with PASCAL > primary antibody 60 minutes, dilution 1:125 Rabbit envision DAB plus > > Hope this helps! > > Cheryl Cross, DVM, Dipl. ACVP > Researcher > University Corporation for Atmospheric Research College of Veterinary Medicine > University of Tennessee Department of Pathology 2407 River Drive, Room A201 > Knoxville, TN 37996-4542 > (423) 967-2724 fax: 865-974-5616 ccross@ucar.edu > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.5.485 / Virus Database: 269.13.5/990 - Release Date: 9/4/2007 10:36 > PM > > No virus found in this outgoing message. > Checked by AVG Free Edition. > Version: 7.5.485 / Virus Database: 269.13.5/990 - Release Date: 9/4/2007 10:36 > PM > > ------------------------------ > > Message: 12 > Date: Wed, 05 Sep 2007 16:13:36 -0400 > From: Sarah Clatterbuck Soper > Subject: [Histonet] Freezing mouse testes for frozen sections > To: histonet@lists.utsouthwestern.edu > Message-ID: <46DF0DF0.2030306@ciwemb.edu> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Hi all, > > I've started attempting to section unfixed frozen mouse testes in order > to placate a specific antibody we have to use. I am new to frozen > sections and I'm having trouble with the testes cracking when I freeze > them. I've tried both freezing in isopentane cooled on liquid nitrogen > and an acetone/dry ice slurry. Either way the testes crack, usually one > big crack end to end. Doesn't seem to be as much of a problem with our > mutant testes, which are about 1/3 the size of wild-type. I wish I > could just trim the wild-type down to a smaller size, but obviously > that's not an option! > > Any recommendations? > > Thanks so much! > > Sarah > > ------------------------------ > > Message: 13 > Date: Wed, 5 Sep 2007 15:49:48 -0500 > From: "Mike Pence" > Subject: [Histonet] Olympus BX40 > To: > Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C701@IS-E2K3.grhs.net> > Content-Type: text/plain; charset="us-ascii" > > Need some help, > > I am looking for a 60x objective for an Olympus microscope BX40. > Would anyone know where I might get a used one or if they even make one > this size for this scope? > > Thanks, > Mike > > ------------------------------ > > Message: 14 > Date: Wed, 5 Sep 2007 16:58:12 -0400 > From: "Yu, Jian" > Subject: [Histonet] Look for a used stereoscope > To: > Message-ID: > <7E0A77BFEB9A1E47A63F978E7116821F07986D6D@1upmc-msx11.acct.upmchs.net> > Content-Type: text/plain; charset="us-ascii" > > Does anyone know a good place to get a used stereoscope? I plan to use > it to examine intestinal tumors in mice. > > Thanks a lot for your information. > > ******************************************************************* > Jian Yu, Ph.D. > University of Pittsburgh Cancer Institute > Hillman Cancer Center Research Pavilion > Office Suite 2.26h > 5117 Centre Avenue, Pittsburgh, PA 15213 > ******************************************************************* > > ------------------------------ > > Message: 15 > Date: Thu, 6 Sep 2007 09:44:45 +1000 > From: "Tony Henwood" > Subject: RE: [Histonet] RDO decal > To: "Rene J Buesa" , "RENEE FISHER" > , > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > There is also the issue of EDTA, which is common in many RDO formulations. > Checking one MSDS for EDTA reveals: > > Ecological Information > Environmental Fate: > When released into the soil, this material is expected to leach into > groundwater. When released into the soil, this material may biodegrade to a > moderate extent. When released into the soil, this material is not expected > to evaporate significantly. When released into water, this material is not > expected to evaporate significantly. This material is not expected to > significantly bioaccumulate. When released into the air, this material is > expected to be readily degraded by photolysis. Environmental Toxicity: > This material is not expected to be toxic to aquatic life. The LC50/96-hour > values for fish are over 100 mg/l. > > So neutralisation may not be required. > If anyone has info to the contrary please advise. > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) > Laboratory Manager & Senior Scientist > The Children's Hospital at Westmead, > Locked Bag 4001, Westmead, 2145, AUSTRALIA. > Tel: 612 9845 3306 > Fax: 612 9845 3318 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, 6 > September 2007 1:07 AM To: RENEE FISHER; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] RDO decal > > Ren?e: > I have not heard of neutralizing RDO, but it would make sense if you want to > be "gentle on your sewer system" BUT prepare to a large emission of carbon > dioxide when attempting to neutralize it with baking soda. RDO + baking soda > (or sodium bicarbonate) will produce water, salt with the acid in RDO > (probably sodium chloride or common salt), and carbon dioxide in > stoichiometrical amounts (1 CO2 per every 1 NaCl). Therefore that > neutralization has to take place in a fumes hood, and you will have to decide > which is worst: delivering acid to the sewer system, or carbon dioxide (the > Greenhouse gas per excellence) to the atmosphere. It will be "your call". > Ren? J. > > RENEE FISHER wrote: > Has anyone heard of neutralizing RDO with baking soda to P.H. 7.0. Our Histo > lab had a hazardous waste assessment, and the consultant suggested we not > throw the RDO down the drain but that we either collect it for waste removal > or neutralize it with baking soda to p.h. 7.0. I have not heard of doing this > and do not know of any procedure for it, everyone, anyone's help will be > greatly appreciated. > > Thanks, > Renee' > > ____________________________________________________________________________ ___________ > > This email may contain confidential protected health information and/or > proprietary information belonging to the sender that is legally privileged > under local, state, or federal law. This information is intended only for the > use of the individual or individuals who have received this. The authorized > recipient of this information is prohibited from disclosing this information > to any other party unless required to do so by law. If you are not the > intended recipient, you are hereby notified that any disclosure, copying, > distribution, or action taken in reliance on the contents of this email is > strictly prohibited. If you have received this email in error, please notify > the sender immediately to arrange for the disposal of this information. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > --------------------------------- > Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: mail, news, > photos & more. _______________________________________________ Histonet > mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************* > This email and any files transmitted with it are confidential and intended > solely for the use of the individual or entity to whom they are addressed. If > you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the > individual sender, and are not necessarily the views of The Children's > Hospital at Westmead > > This note also confirms that this email message has been > virus scanned and although no computer viruses were detected, The Childrens > Hospital at Westmead accepts no liability for any consequential damage > resulting from email containing computer viruses. ********************************************************************** > > ------------------------------ > > Message: 16 > Date: Thu, 6 Sep 2007 11:23:00 +0900 > From: ChoiUl Soo > Subject: [Histonet] PTH antibody reacted with dog tissue... > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="ks_c_5601-1987" > > Hi Histonetters, > > I am interested in anti PTH antibody reacted with dog tissue. > If anyone has successful experience with any PTH antibody with dog tissue, > please tell me one. > > I have searched through the internet, and results came back with abcam and > SantaCruz PTH antibodies predicted to react with them. But they are not sure > on it, just predicted on the basis of sequence homology. > (abcam say 94% homology with human) I don't have good exprience with Santa > Cruz, and never used one by Abcam. Should I rely on it, or find another one? > > Let me hear your experience. > > I would appreciate your advice or comment on this. > > Thank you~. > > Ul Soo Choi, DVM, PhDKRF priority zoonotic disease research institute, College > of Veterinary Medicine, Seoul National University, Shilim9 dong, Gwanakgu, > Seoul, Korea 151-742Tel. 82-02-880-8688 Mobile. 82-016-9228-8634Fax. 82-02-880- > 8662 > > _________________________________________________________________ > ???? ?????? ????, Windows Live Space! > http://www.spaces.live.com > > ------------------------------ > > Message: 17 > Date: Thu, 6 Sep 2007 01:57:04 -0400 > From: "Michelle McCoy" > Subject: [Histonet] Floater sources > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <25355ef80709052257i32794198gd5a64b94758cc1ea@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > I was reading some of the old archived messages on floaters, and being > somewhat new to the field of histotechnology was curious about how floaters > can be attributed to a particular tech who performed work on the block. For > example, couldn't other sources of contamination be from the automatic > stainer, processor carryover (cassette not completely closed/or if closed, > friable tissue through the slats eg if sponge not used on larger specimen). > In some previous labs I've worked in- floaters were quickly attributed to > the cutter, embedder or grosser and might result in a "write up". If the > floater is seen in the block how can you differentiate if it came from the > grosser carryover/embedder carryover/processing carryover/unsigned > re-embedder/or even possibly even client carry over between patients or > different specimen types of the same patient. > And if it is not in the block --differentiating between the cutter/automatic > stainer (I've seen specks of tissue debris in automatic stainers/ sections > falling off the slides and into the reagents etc). Obviously all should be > done to minimize the factors, but I'm just not clear how a single source is > pinpointed and potentially blamed for the event (depending on the lab > policy). Thanks for any ideas on this. > > ------------------------------ > > Message: 18 > Date: Thu, 6 Sep 2007 08:07:38 +0100 > From: "Kemlo Rogerson" > Subject: RE: [Histonet] Xylene > To: , > Message-ID: > <86ADE4EB583CE64799A9924684A0FBBF0222EC6C@wahtntex2.waht.swest.nhs.uk> > Content-Type: text/plain; charset="us-ascii" > > Can somebody tell me if there are any effects on tissue if there is > prolonged processing time in xylene? > > Jason McGough HT(ASCP) > Clinical Laboratory of the Black Hills > Account Representative - Anatomic Pathology > 2805 5th Street > Rapid City, SD 57701 > 605-343-2267 > jmcgough@clinlab.com > > Classically it is said to harden, plus more lipids could be removed. > Personally I'm equivocal about that thought; if properly fixed prior to > processing I would have thought the hardening effects of xylene were > much less than that of the coagulant fixative ethanol. If you do have > hard tissue then there is a restorative fluid one can use which I think > has oil of cedarwood in it but I don't know the formula off hand. I know > it works cos when I was a pup I used it sometimes. > > Kemlo Rogerson > Pathology Manager > DD 01934 647057 or extension 3311 > Mob 07749 754194; Pager 07659 597107; > > Sunshine is delicious, rain is refreshing, wind braces us up, snow is > exhilarating; there is really no such thing as bad weather, only > different kinds of good weather. --John Ruskin > > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. > Please inform me that this message has gone astray before deleting it. > Thank you for your co-operation > > ------------------------------ > > Message: 19 > Date: Thu, 6 Sep 2007 08:17:36 +0100 > From: "Kemlo Rogerson" > Subject: RE: [Histonet] Floater sources > To: "Michelle McCoy" , > > Message-ID: > <86ADE4EB583CE64799A9924684A0FBBF0222EC6D@wahtntex2.waht.swest.nhs.uk> > Content-Type: text/plain; charset="us-ascii" > > I was reading some of the old archived messages on floaters, and being > somewhat new to the field of histotechnology was curious about how > floaters can be attributed to a particular tech who performed work on > the block. For example, couldn't other sources of contamination be from > the automatic stainer, processor carryover (cassette not completely > closed/or if closed, friable tissue through the slats eg if sponge not > used on larger specimen). In some previous labs I've worked in- floaters > were quickly attributed to the cutter, embedder or grosser and might > result in a "write up". If the floater is seen in the block how can you > differentiate if it came from the grosser carryover/embedder > carryover/processing carryover/unsigned re-embedder/or even possibly > even client carry over between patients or different specimen types of > the same patient. And if it is not in the block --differentiating > between the cutter/automatic stainer (I've seen specks of tissue debris > in automatic stainers/ sections falling off the slides and into the > reagents etc). Obviously all should be done to minimize the factors, but > I'm just not clear how a single source is pinpointed and potentially > blamed for the event (depending on the lab policy). Thanks for any ideas > on this. > > You are exactly correct, floaters can be attributed to a variety of > causes, processing machines that aren't regularly changed, transfer on > the cutting up forceps, on the waterbath and on the forceps of the > embedder. If the floater was from a block that was cut, embedded, cut up > before the section with the floater then the culprit is obvious. If the > section was cut by someone who didn't cut the block from which the > floater floated, then the culprit is the embedder, machine,or cutter up. > Realisticaly I would have thought that most floaters would be from the > embedder, cutter up, then sectioner as the latter has the greater > likelihood of seeing the error of his/ her ways. Waxy forceps of messy > forceps, in my experience are the usual culprit but in some instances, > necrotic tumours can shed cells in the processor and even the stainer. > > Floaters are usually obvious as they tend to be at a different level > than the tissue section and I'm afraid they are a fact of life. You can > reduce the incidence but sadly never eradicate them. > > Kemlo Rogerson > Pathology Manager > DD 01934 647057 or extension 3311 > Mob 07749 754194; Pager 07659 597107; > > Sunshine is delicious, rain is refreshing, wind braces us up, snow is > exhilarating; there is really no such thing as bad weather, only > different kinds of good weather. --John Ruskin > > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. > Please inform me that this message has gone astray before deleting it. > Thank you for your co-operation > > ------------------------------ > > Message: 20 > Date: Thu, 6 Sep 2007 04:18:04 -0400 > From: "Victoria Baker" > Subject: [Histonet] Fwd: Users of Lab Vision autostainer > To: histonet > Message-ID: > <4f016b690709060118t532d22c1if250116e0a6c9af1@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > ---------- Forwarded message ---------- > From: Victoria Baker > Date: Sep 5, 2007 9:02 AM > Subject: Users of Lab Vision autostainer > To: Histo Net list server > > Hi > > I'm a new user of the Lab Vision autostainer and I'm looking to see if > I can find users in Histo-land that have experience with it. The > facility only works with human tissue and all of the antibodies are > for dx purposes. > > My key questions are as follows: > How many antibodies is your lab running? > How many users do you allow? > How many people do you allow programming rights and at what level of > supervision are they? > > How many of these antibodies are from Lab Vision? > a) are they concentrates or pre-dilutes? > b) for HIER are you using their PT modules/procedures or > your own equipment (microwave, steamer, pressure cooker etc) and in > house designed protocols for retrieval? > c) for digestion do you only use their Pro-K or have you > designed your own in-house methods using other reagents for digestion? > d) do you put your controls on the same slide as the patient? > e) are your controls in-house or commercial? > f) do you have more than one stainer hooked up to one computer system? > > What Version of software do you currently have on your system? > > Any feed back would be very helpful. > > Thanks in advance. > > Vikki Baker > Interim Histology Manager > Mission Hospital System > Asheville, NC > > ------------------------------ > > Message: 21 > Date: Thu, 6 Sep 2007 02:28:57 -0600 > From: "Joseph Kapler" > Subject: RE: [Histonet] Xylene > To: "Rene J Buesa" , , > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Tissues processed in Xylene (especially over long periods of time) the > tissue becomes very brittle. > > Hope that is the response you were looking for. > > Joseph "DarkWolfe" Kapler > I'm an outsider outside of everything > I'm an outsider outside of everything > I'm an outsider outside of everything > Everything you know. Everything you know > It disturbs me so > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J > Buesa > Sent: Wednesday, September 05, 2007 14:01 > To: themagoos@rushmore.com; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Xylene > > Tissues usually become brittle, which difficulties sectioning. > Ren? J. > > themagoos wrote: > Can somebody tell me if there are any effects on tissue if > there is prolonged processing time in xylene? > > Jason McGough HT(ASCP) > Clinical Laboratory of the Black Hills > Account Representative - Anatomic Pathology > 2805 5th Street > Rapid City, SD 57701 > 605-343-2267 > jmcgough@clinlab.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > --------------------------------- > Boardwalk for $500? In 2007? Ha! > Play Monopoly Here and Now (it's updated for today's economy) at Yahoo! > Games. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.5.485 / Virus Database: 269.13.5/988 - Release Date: 9/4/2007 > 09:14 > > No virus found in this outgoing message. > Checked by AVG Free Edition. > Version: 7.5.485 / Virus Database: 269.13.5/988 - Release Date: 9/4/2007 > 09:14 > > ------------------------------ > > Message: 22 > Date: Thu, 6 Sep 2007 09:59:13 +0100 > From: Malam Jacqueline > Subject: [Histonet] Unsubscribe > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain > > Please would you unsubscribe me as I am retiring tomorrow - thanks > > Jacqui malam > Lancaster > uk > > DISCLAIMER: This e-mail is confidential and privileged. If you are not the > intended recipient please accept our apologies; please do not disclose, copy > or distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. Please > inform postmaster@rli.mbht.nhs.uk that this message has gone astray before > deleting it. Comments or opinions expressed in this email are those of > their respective contributors only. The views expressed do not represent the > views of the Trust, its management or employees. University Hospitals of > Morecambe Bay NHS Trust is not responsible and disclaims any and all > liability for the content of comments written within.Thank you for your > co-operation. > > ------------------------------ > > Message: 23 > Date: Thu, 6 Sep 2007 06:31:58 -0500 > From: "Joe Nocito" > Subject: Re: [Histonet] Floater sources > To: "Michelle McCoy" , > > Message-ID: <004201c7f079$8f5aa560$0202a8c0@yourxhtr8hvc4p> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > Michelle, > first. welcome to world of histology. > Let's begin at the grossing table- all grossers are trained to wipe off the > table after each case- but that doesn't mean floaters can't happen, but the > incidence is low > third- embedders should have been taught to clean the embedding area after > each case and at the end of the day, but this is a good source for > contamination. You can see this is the block. > fourth- the cutters should wipe off their waterbaths after each block. This > probably is the most likely source of contamination. I've inspected some > labs where one waterbath was just covered with previous ribbons, attached to > the side and floating on the waterbath. > fifth- in my experience, it is highly unlikely that floaters came from the > stainer just by the shear movement of the slides and water. > > The bottom line is that everyone needs to be neat and clean and be > meticulous. Remember, cleanliness is next to Godliness. > I had a testicular seminoma where no matter what we did, floaters still were > on cervical bxs and endometrial bxs. The medical and I decided that we > would handle cases like these separately. They were processed, embedded, cut > and stained separately. Once the case was completed, we changed all the > solutions on the tissue processor and stainer. Who ever cut the blocks had > to dismantle their knife holder and microtome to clean it thoroughly. A pain > in the butt, but was a necessary evil. > > I hope this helped a little. Good luck. > > Joe The Toe > ----- Original Message ----- > From: "Michelle McCoy" > To: > Sent: Thursday, September 06, 2007 12:57 AM > Subject: [Histonet] Floater sources > > >I was reading some of the old archived messages on floaters, and being > > somewhat new to the field of histotechnology was curious about how > > floaters > > can be attributed to a particular tech who performed work on the block. > > For > > example, couldn't other sources of contamination be from the automatic > > stainer, processor carryover (cassette not completely closed/or if closed, > > friable tissue through the slats eg if sponge not used on larger > > specimen). > > In some previous labs I've worked in- floaters were quickly attributed to > > the cutter, embedder or grosser and might result in a "write up". If the > > floater is seen in the block how can you differentiate if it came from the > > grosser carryover/embedder carryover/processing carryover/unsigned > > re-embedder/or even possibly even client carry over between patients or > > different specimen types of the same patient. > > And if it is not in the block --differentiating between the > > cutter/automatic > > stainer (I've seen specks of tissue debris in automatic stainers/ sections > > falling off the slides and into the reagents etc). Obviously all should be > > done to minimize the factors, but I'm just not clear how a single source > > is > > pinpointed and potentially blamed for the event (depending on the lab > > policy). Thanks for any ideas on this. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 46, Issue 6 > *************************************** ------- End of Original Message ------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From garth <@t> apollosci.co.za Fri Sep 7 00:56:09 2007 From: garth <@t> apollosci.co.za (Garth Jerome) Date: Fri Sep 7 00:56:40 2007 Subject: [Histonet] Microwaves VENDOR RESPONSE In-Reply-To: <46E00B4D.6E1B.0061.0@lhsc.on.ca> Message-ID: <000101c7f113$cb0b5a60$7700a8c0@jhb.apollosci.co.za> Hello All Just a short 'five cents worth': We have found that since the purchase of our microwave processor, our processing has turned the corner. There is a strong commercial argument for a microwave processor and that is, if a laboratory makes full use of the instrument for both processing and value-added processes like antigen retrieval, then the instrument becomes a good investment. Simply purchasing a laboratory grade microwave for AR only, can appear fairly wasteful. Our unit is used for routine processing, decalicification and AR and special stains. Due to the design, we can carry out multiple functions at once. We also have saved a fair amount of money on reagents. Regards Garth Jerome Apollo Scientific cc Telephone : 27-11-466 7666 Facsimile : 27-11-466 7672 Cell phone : 084 504 1101 Email : garth@apollosci.co.za -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Doug Geddes Sent: 06 September 2007 08:15 PM To: Kathleen Boozer; Jeanine (CDC/CCID/NCZVED) Bartlett; Phil McArdle; Joe Nocito Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Microwaves VENDOR RESPONSE At present we are using the TT Mega for antigen retrieval only. Doug Geddes BSc, MLT Department of Pathology London Helath Sciences Centre London, ON Canada >>> "Bartlett, Jeanine (CDC/CCID/NCZVED)" 2007-09-06 11:58 AM >>> Do you use the TT/Mega just for special staining? Jeanine Bartlett Infectious Disease Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Doug Geddes Sent: Thursday, September 06, 2007 11:48 AM To: Kathleen Boozer; Phil McArdle; Joe Nocito Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Microwaves VENDOR RESPONSE Sorry Joe, but budgets aside, I think you get what you pay for. We are a high volume lab and have gone through at least 8 "Wal Mart" microwaves in the last 5 years. We now have the TT Mega, and it is wonderful, it is all about control as much as you can. Morphology is amazing. Doug Geddes BSc, MLT Department of Pathology London Helath Sciences Centre London, ON Canada >>> "Joe Nocito" 2007-09-05 11:56 AM >>> ok, but with the budgets today, many people can't afford a $1749 microwave when they can buy one at Walmart, K-Mart, or somewhere else for $79. Not to make you angry or anything, but I'm wondering how long has it been since you worked in a lab? Histo's budget is the first one cut in the lab because we are not essential. I can't count how many times I fought and fought for my budgets. If I tried to justify a $1749 microwave for special stains, HIER or whatever, I would have been laughed out the manager's office. Just my 4 cents. JTT ----- Original Message ----- From: "Phil McArdle" To: "Kathleen Boozer" Cc: Sent: Wednesday, September 05, 2007 9:07 AM Subject: Re: [Histonet] Microwaves VENDOR RESPONSE > Again, a microwave vendor weighs in (so far I haven't received any > flames), so read at your own risk. :-) > > At the risk of sounding overly and overtly commercial, after reading post > after post of $30,000+ and $18,000 and similarly high figures for lab > microwaves, I really feel the need to set the record straight. Depending > on the usage requirements, we have laboratory microwaves as low as $1749 > for a "bare bones" model for simple operations, to mid-priced units, to > under $11,000 for a vacuum equipped microwave processor capable of the +/- > 0.5 degree C temperature control necessary for tissue processing. > > (I can feel the heat already!) > > There are many compelling reasons to replace a kitchen microwave with a > lab model; feel free to download, read, and even share with colleagues our > Microwave Companion at > > http://www.ebsciences.com/pdf/EBS_MW_COMPANION.pdf > > Best regards, and see you at NSH, > > Phil McArdle > > -- > Phil McArdle > Microwave Product Manager > > Energy Beam Sciences, Inc. > 29-B Kripes Rd. > East Granby, CT 06026 > > Tel: 800.992.9037 x 341 > Mobile: 860.597.6796 > Fax: 860.653.0422 > > pmcardle@ebsciences.com > www.ebsciences.com > > Kathleen Boozer wrote: >> What is the best microwave for a small lab using it only for heating >> Bouin's and Silver Nitrate for special stains? I just can't believe I >> would have to spend $30,000+ or slow down and use a waterbath. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > I skate to where the puck is going to be, not to where it's been. > - Wayne Gretsky > > You must be the change you want to see in the world. > - Mahatma Gandhi > > NOTE: This message, together with any attachments, is intended only for > the use of the individual or entity to which it is addressed and may > contain information that is legally privileged, confidential and exempt > from disclosure. If you are not the intended recipient, however, there's > not a lot I can do about it, and it was probably my mistake anyway. So > please do the right thing and make this e-mail go away. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sonya.martin <@t> soton.ac.uk Fri Sep 7 03:27:19 2007 From: sonya.martin <@t> soton.ac.uk (James S.) Date: Fri Sep 7 03:27:51 2007 Subject: [Histonet] RE:Acetone fixation for immunofluorescence protocols Message-ID: <71437982F5B13A4D9A5B2669BDB89EE41074D1BC@ISS-CL-EX-V1.soton.ac.uk> Mauricio, You didn't say what your problem was - is it the integrity of your sections or the fluorescence staining? I always use acetone fixation for mouse tissues for immunofluorescence and havent had any problems with the labelling however I have had problems on and off with the integrity of the sections especially after long labelling procedures. To combat this I make sure the acetone is 'dry' (I use molecular sieves), I dry the sections for at least 1hr (sometimes overnight) after cutting and try to keep incubation times to a minimum. I still occasionally have problems with the sections disintegrating during labelling so I'd be interested to hear anyone elses comments! Sonya From Terry.Marshall <@t> rothgen.nhs.uk Fri Sep 7 05:45:16 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 7 05:45:38 2007 Subject: [Histonet] IHC staining weaker in... Message-ID: <407F05A128805F4C879A33DBA32E618E01895031@TRFT-EX01.xRothGen.nhs.uk> Everyone is shouting fixation problem, but is this something we see? I don't. The only block that regularly shows something like this is a lymph node that has been put in intact, but there, there is the additional autolysis, which the questioner did not mention. In addition, I gather from the question, that this is a general fault. WE perhaps need more information perhaps. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: 06 September 2007 21:35 To: Marshall Terry Dr, Consultant Histopathologist; Department of Pathology; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC staining weaker in... sounds like a fixation problem. Joe ----- Original Message ----- From: "Marshall Terry Dr, Consultant Histopathologist" To: "Department of Pathology" ; Sent: Thursday, September 06, 2007 11:14 AM Subject: RE: [Histonet] IHC staining weaker in... I thought I had seen every horror possible in a section, but that seems a new one to me! Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Department of Pathology Sent: 06 September 2007 16:48 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC staining weaker in... Dear Participants, our diagnostic pathology lab has a problem. The paraffin sections are stained weakly at the center but better at the periphery of the sections. What could be the reason.??... thanks a lot.. Dr Nasuhi Engin Aydin, malatya Inonu University Hospital, Turkey 44365. ---------- Original Message ----------- From: histonet-request@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Thu, 6 Sep 2007 14:51:24 +0300 (EEST) Subject: Histonet Digest, Vol 46, Issue 6 > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > Today's Topics: > > 1. Xylene (themagoos) > 2. RE:The great apoptosis stains debate (TUNEL, Caspases, etc) > (Melissa Gonzalez) > 3. Re: RDO decalcifier (Robert Richmond) > 4. Re: IHC anti-cleaved caspase 3 (Carl Hobbs) > 5. avian sperm cell topography (melissah rowe) > 6. Re: IHC anti-cleaved caspase 3 (Thomas Pier) > 7. Re: IHC anti-cleaved caspase 3 (Cheryl Cross) > 8. Fwd: Users of Lab Vision autostainer (Victoria Baker) > 9. Re: Microwaves VENDOR RESPONSE (Phil McArdle) > 10. Re: Xylene (Rene J Buesa) > 11. RE: IHC anti-cleaved caspase 3 (Liz Chlipala) > 12. Freezing mouse testes for frozen sections > (Sarah Clatterbuck Soper) > 13. Olympus BX40 (Mike Pence) > 14. Look for a used stereoscope (Yu, Jian) > 15. RE: RDO decal (Tony Henwood) > 16. PTH antibody reacted with dog tissue... (ChoiUl Soo) > 17. Floater sources (Michelle McCoy) > 18. RE: Xylene (Kemlo Rogerson) > 19. RE: Floater sources (Kemlo Rogerson) > 20. Fwd: Users of Lab Vision autostainer (Victoria Baker) > 21. RE: Xylene (Joseph Kapler) > 22. Unsubscribe (Malam Jacqueline) > 23. Re: Floater sources (Joe Nocito) > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 05 Sep 2007 10:21:51 -0700 > From: "themagoos" > Subject: [Histonet] Xylene > To: histonet@lists.utsouthwestern.edu > Message-ID: <46dee5af.14b.450a.1030817715@rushmore.com> > Content-Type: text/plain; charset="iso-8859-1" > > Can somebody tell me if there are any effects on tissue if > there is prolonged processing time in xylene? > > Jason McGough HT(ASCP) > Clinical Laboratory of the Black Hills > Account Representative - Anatomic Pathology > 2805 5th Street > Rapid City, SD 57701 > 605-343-2267 > jmcgough@clinlab.com > > ------------------------------ > > Message: 2 > Date: Wed, 5 Sep 2007 11:30:38 -0700 > From: "Melissa Gonzalez" > Subject: [Histonet] RE:The great apoptosis stains debate (TUNEL, > Caspases, etc) > To: > Message-ID: > <2884B897182A1D438C7BA24B9A8F94A20E701D@hqsvr01mail.cgi.com> > Content-Type: text/plain; charset="iso-8859-1" > > Hi all, > I have been around this mess a few years back, I gave up on TUNEL long ago > for > the various reasons mentioned on the list recently. > > Per investigators requests, I have tried several ways to demonstrate cell > death, however methods such as PI or Annexin staining are more suitable > for > whole cells, not tissue sections. I have also not had any luck staining > for > Cytochrome C. > > We routinely use R&D Systems rabbit anti human/mouse active Caspase3 using > a > high pH (EDTA) based Ag retrieval in a steamer (enzymes do not work). > Works > like a charm. > > Any other suggestions? I know this topic has been thrown around a lot, but > these discussions after all, help us get our jobs done more proficiently. > > Melissa > > Melissa A. Gonz?lez Edick > R&D, Cell Genesys Inc. > 500 Forbes Blvd > South San Francisco, CA 94080 > p(650) 266-3168 > f (650) 266-3080 > > "It's not enough to believe what you see, you must also understand what > you > see." -Leonardo Da Vinci > ------------------------------ > > Message: 6 > Date: Tue, 4 Sep 2007 15:18:39 -0400 > From: Cheryl Cross > Subject: Re: [Histonet] TUNEL, Formalin, DNA strand breaks > To: JR R > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <4C1E074D-064A-45D1-BD32-13D7E2222B26@aol.com> > Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed > > Hi all - > > I have been round and round with this issue myself; I was basically > told by people who've attempted it that TUNEL on FFPE sections is not > specific enough due to the formalin issue. > > If you are trying to nail apoptosis, what about anti-active > caspase-3? mind you, that staining can be a bit of a booger too, but > it should be more sensitive and specific for apoptosis (no references > to offer, i'm just repeating what i have been told). > > Cheryl Cross, DVM, Dipl. ACVP > Researcher > University Corporation for Atmospheric Research > College of Veterinary Medicine > University of Tennessee Department of Pathology > 2407 River Drive, Room A201 > Knoxville, TN 37996-4542 > (423) 967-2724 > fax: 865-974-5616 > ccross@ucar.edu > ------------------------------ > > Message: 5 > Hi Ray, > > Sheesh-- I read (and then lost) the paper maybe 5-7 years ago, back when I > was > tearing my hair out over high background, or false positives for TUNEL > stain > in formalin fixed, paraffin embedded arterial sections. I can > troubleshoot > any immunostain, but the TUNEL and ISEL assays bedeviled me. > > The paper was titled something to the effect of "formaldehyde causes > single > and double stranded DNA breaks," and was pretty emphatic. I'll look for > the article. > > I think the assay could work in whole cells for flow, or maybe in frozen > tissue. My hunch at this point is that this is one of those rare cases > where > lots and lots of peer reviewed articles are just plain wrong. > > Hey, if someone has a good protocol, I'd be willing to try it out, and I > would > be delighted if I turn out to be mistaken. For now, I think TUNEL is the > assay of the beast. > > Oh, here is a thought--say you are looking at a 5 micron section through a > nucleus. The microtome blade pretty much had to create a lot of double > stranded DNA breaks, no? > > Jerry L. Ricks > Research Scientist > U.W. Medicine at South Lake Union > 815 Mercer Street > Seattle, WA 98109 > (206)-685-7190 > > From: koellingr@comcast.netTo: rosenfeldtek@hotmail.com; > histonet@lists.utsouthwestern.eduSubject: RE: [Histonet] TUNELDate: Fri, > 31 > Aug 2007 23:17:03 +0000 > > Jerry, > Could you expand on or give references to formalin causing DNA strand > breaks? > Double strand breaks, single strand, blunt end, overhanging? My pile of > papers and having done TUNEL for years says that formalin fixation is a > very > good technique for TUNEL and many peer-reviewed articles in which TUNEL is > used as a technique, use formalin fixation and how can that be if formalin > is > causing strand breaks? In fact one paper I'm looking at says that > extended (5- > 7 weeks in formalin) fixation causes loss of TUNEL signal. If formalin is > causing breaks, you would assume that TUNEL pos signals would increase > with > extended formalin fixation. Even the use of the monoclonal antibody > F7-26, > for single stranded DNA, touts formalin fixation for their claims of > discriminating apoptosis from necrosis. > > The question asks about extended alcohol fixation but your answer is > possibly > a lot of false positives because formalin causes DNA breaks. Does this > imply > that alcohol won't? Have done a lot of TUNEL on alcohol fixed samples. > True > I couldn't pretreat them and handle them they way I would handle FFPE > tissue. > Also true that we could argue specificity and ability or not to > discriminate > apoptosis from necrosis for quite a while. But if formalin itself is > causing > the breaks in DNA, I and a lot of people are in big trouble with our > science > projects and experiments. Also I can't envision why 2 cells are showing > TUNEL > positivity while 2 of the same type of cells right next to them (and > getting > the same formalin fix), are absolutely clean and there is no background? > > Thanks for any information you can provide. > > Ray Koelling > PhenoPath Laboratories > Seattle, WA > > ------------------------------ > > Message: 3 > Date: Wed, 5 Sep 2007 14:56:14 -0400 > From: "Robert Richmond" > Subject: [Histonet] Re: RDO decalcifier > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > I don't see how your hazmats people can tell you what to do to dispose > of RDO, since nobody has any idea what's in it. It's a murky yellow > liquid that continuously throws a black sediment that has to be > filtered out if you're to see your specimen in it. It's sort of the > ultimate secret formula, and I've always wondered why it's so popular > - I've had to use it many different places in my travels. > > I'd suggest either an ordinary proprietary decalcifier that's a clear > liquid, probably hydrochloric acid - or else save money and dilute > your own hydrochloric acid. You can re-use it for a while. When the > time comes to dispose of it, it can be safely diluted with a lot of > water and put down the drain. > > Remember - it's been stressed on this list many times - that "Decal" > is a brand name still in use, and that other brands of decalcifying > solution should not be called Decal. > > Bob Richmond > Samurai Pathologist (never a decalcified Carmelite) > Knoxville TN > > ------------------------------ > > Message: 4 > Date: Wed, 5 Sep 2007 20:29:36 +0100 > From: "Carl Hobbs" > Subject: Re: [Histonet] IHC anti-cleaved caspase 3 > To: "Histonet" > Message-ID: <001801c7eff3$18894850$4101a8c0@carlba65530bda> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > Hi. > > Recent Cleaved caspase 3 posts have not mentioned the antibody details: I > would be grateful for the source of these Abs. > Carl > > ------------------------------ > > Message: 5 > Date: Wed, 5 Sep 2007 14:36:50 -0500 > From: melissah rowe > Subject: [Histonet] avian sperm cell topography > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed > > Hi, > > I am a PhD student working on a behavioral ecology project on > Australian fairy-wrens. I am currently trying to find out if I can > determine the dimensions of discrete sections of individual sperm > cells, i.e. acrosome length, nucleus length, midpiece length and tail > length using the material I currently have available. I have prepared > slide smears of sperm that has been previously stained with an eosin- > nigrosin stain (to determine cell viability) and would like to be > able to somehow stain different segments of the sperm so that I can > identify and measure them using an ocular micrometer on a bright > field or phase contrast microscope set up. It is my understanding > that I cannot use fluorescent stains given that I have already used a > colormetric stain (the eosin-nigrosin). If anyone has suggestions for > ways to identify discrete regions of avian (passerine) sperm cells I > would be very grateful to hear from you. > > Many thanks in advance, > melissah > > ----- > melissah rowe > > PhD candidate > Department of Ecology & Evolution > University of Chicago > E. 57th Street, Chicago, IL, 60637 > ph: +1 773-702-3070 > fax: + 1 773-702-9740 > > email: melissah@uchicago.edu > > ------------------------------ > > Message: 6 > Date: Wed, 05 Sep 2007 14:39:04 -0500 > From: "Thomas Pier" > Subject: Re: [Histonet] IHC anti-cleaved caspase 3 > To: , > Message-ID: <46DEBF88020000DF0000A736@gwmail.medicine.wisc.edu> > Content-Type: text/plain; charset=US-ASCII > > Cell Signalling Technology has a good rabbit monoclonal for Cleaved > Caspase-3. > > Tom Pier > > >>> "Carl Hobbs" 09/05/07 2:29 PM >>> > Hi. > > Recent Cleaved caspase 3 posts have not mentioned the antibody details: I > would be grateful for the source of these Abs. > Carl > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 7 > Date: Wed, 5 Sep 2007 15:54:35 -0400 > From: Cheryl Cross > Subject: Re: [Histonet] IHC anti-cleaved caspase 3 > To: "Thomas Pier" > Cc: carl.hobbs@kcl.ac.uk, histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed > > I will second the Cell Signaling antibody - we have tried antibodies > from Promega (which gave tons of background staining)...then via this > site i was pointed to Cell Signaling's monoclonal which we tried; the > polyclonal actually has been working very well with minimal > background (we were getting lots of respiratory epithelium and > endothelium lighting up in control animals). I have been using it in > mice with this protocol: > > EDTA with PASCAL > primary antibody 60 minutes, dilution 1:125 > Rabbit envision > DAB plus > > Hope this helps! > > Cheryl Cross, DVM, Dipl. ACVP > Researcher > University Corporation for Atmospheric Research > College of Veterinary Medicine > University of Tennessee Department of Pathology > 2407 River Drive, Room A201 > Knoxville, TN 37996-4542 > (423) 967-2724 > fax: 865-974-5616 > ccross@ucar.edu > > > > > ------------------------------ > > Message: 8 > Date: Wed, 5 Sep 2007 15:59:45 -0400 > From: "Victoria Baker" > Subject: [Histonet] Fwd: Users of Lab Vision autostainer > To: "Histo Net list server" > Message-ID: > <4f016b690709051259rcdffccet70d7f97ae0457707@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > One more try, first one didn't go through it seems! Thanks > > ---------- Forwarded message ---------- > From: Victoria Baker > Date: Sep 5, 2007 9:02 AM > Subject: Users of Lab Vision autostainer > To: Histo Net list server > > Hi > > I'm a new user of the Lab Vision autostainer and I'm looking to see if > I can find users in Histo-land that have experience with it. The > facility only works with human tissue and all of the antibodies are > for dx purposes. > > My key questions are as follows: > How many antibodies is your lab running? > How many users do you allow? > How many people do you allow programming rights and at what level of > supervision are they? > > How many of these antibodies are from Lab Vision? > a) are they concentrates or pre-dilutes? > b) for HIER are you using their PT modules/procedures or > your own equipment (microwave, steamer, pressure cooker etc) and in > house designed protocols for retrieval? > c) for digestion do you only use their Pro-K or have you > designed your own in-house methods using other reagents for digestion? > d) do you put your controls on the same slide as the patient? > e) are your controls in-house or commercial? > f) do you have more than one stainer hooked up to one computer > system? > > What Version of software do you currently have on your system? > > Any feed back would be very helpful. > > Thanks in advance. > > Vikki Baker > Interim Histology Manager > Mission Hospital System > Asheville, NC > > ------------------------------ > > Message: 9 > Date: Wed, 05 Sep 2007 16:00:47 -0400 > From: Phil McArdle > Subject: Re: [Histonet] Microwaves VENDOR RESPONSE > To: Joe Nocito > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <46DF0AEF.7030203@ebsciences.com> > Content-Type: text/plain; charset=UTF-8; format=flowed > > Hi Joe: > > Obviously, a microwave vendor hates to hear microwave horror stories, so > again, no argument - even though I'm not privy to details of the fried > biopsies in question or what type/vintage of microwave, anyone who's > experienced a malfunction involving patient samples doesn't want a > repeat performance. And pathology is, must be, risk averse. > > That said, again, any mechanical or electronic equipment can fail, or > user error can contribute; just look at the "hang-up" problems with > older tissue processors that are now ancient history. It's up to > manufacturers to minimize the possibilities of failure, since patient > care is at stake. Improvement is therefore a continual, ongoing process. > For example, while for years EBS microwave processors incorporated > safety shutdown modes in the event of vent failure, probe failure (open > and closed) and many other component-related issues, about two years ago > we determined that the microwave did not have a comprehensive set of > safeguards to deal with user errors, for example, temperature overshoots > caused by too small a container for a given power setting, or failure to > place the temperature probe in solution. So we developed multiple safety > mechanisms to head off user errors of this sort. > > PMM > -- > Phil McArdle > Microwave Product Manager > > Energy Beam Sciences, Inc. > 29-B Kripes Rd. > East Granby, CT 06026 > > Tel: 800.992.9037 x 341 > Mobile: 860.597.6796 > Fax: 860.653.0422 > > pmcardle@ebsciences.com > www.ebsciences.com > > Joe Nocito wrote: > > are you sure it's the willies and not the johnnies? > > Since the magnetron or whatever it was that fried my tissue, I'd wait > > for the traditional processing. Call me a dinosaur, but I really don't > > like doing special stains in the microwave. The only thing I use a > > microwave for at my house is to defrost and reheat stuff (technical > > term). I'm sure there are people out there who can cook a 6 course > > gourmet meal. My best friend can process all types of tissue from > > biopsies to uterus. > > As a matter of fact, he was there grossing when something went wrong > > and told me that he's never seen tissue like that before. > > > > JTT > > ----- Original Message ----- From: "Phil McArdle" > > > > To: "Joe Nocito" > > Cc: > > Sent: Wednesday, September 05, 2007 11:16 AM > > Subject: Re: [Histonet] Microwaves VENDOR RESPONSE > > > > > >> Hi Joe: > >> > >> No argument there. I'm painfully aware of both a mindset of "a > >> microwave 'should' cost less than $100," and of a dearth of funding > >> for pathology in general (popular shows like CSI to the contrary). :-) > >> I'd still suggest that $1749 is a heck of a lot better (and a lot less > >> laughable) than the $18,000 or $30,000 that's widely quoted and > >> posted, and it's the exact reason we brought an under-$2000 lab > >> microwave to market in the first place. > >> > >> One could argue just as convincingly against all kinds of specialized > >> equipment or reagents on the basis of cost, not just microwaves. We > >> all know of everything from saliva to cheap rice steamers being used > >> in histo labs, and while they may actually be perfectly serviceable, > >> from the standpoint of repeatability or liability, this kind of thing > >> gives me the willies (and that's a technical term). My yardstick is > >> always "what would I be comfortable with if my kid's diagnosis hung in > >> the balance?" > >> > >> Healthy debate is good! > >> > >> Phil > >> -- > >> Phil McArdle > >> Microwave Product Manager > >> > >> Energy Beam Sciences, Inc. > >> 29-B Kripes Rd. > >> East Granby, CT 06026 > >> > >> Tel: 800.992.9037 x 341 > >> Mobile: 860.597.6796 > >> Fax: 860.653.0422 > >> > >> pmcardle@ebsciences.com > >> www.ebsciences.com > >> > >> > >> > >> > >> > >> > >> Joe Nocito wrote: > >>> ok, but with the budgets today, many people can't afford a $1749 > >>> microwave when they can buy one at Walmart, K-Mart, or somewhere else > >>> for $79. > >>> Not to make you angry or anything, but I'm wondering how long has > >>> it been since you worked in a lab? Histo's budget is the first one > >>> cut in the lab because we are not essential. > >>> I can't count how many times I fought and fought for my budgets. > >>> If I tried to justify a $1749 microwave for special stains, HIER > >>> or whatever, I would have been laughed out the manager's office. > >>> Just my 4 cents. > >>> > >>> JTT > >>> ----- Original Message ----- From: "Phil McArdle" > >>> > >>> To: "Kathleen Boozer" > >>> Cc: > >>> Sent: Wednesday, September 05, 2007 9:07 AM > >>> Subject: Re: [Histonet] Microwaves VENDOR RESPONSE > >>> > >>> > >>>> Again, a microwave vendor weighs in (so far I haven't received any > >>>> flames), so read at your own risk. :-) > >>>> > >>>> At the risk of sounding overly and overtly commercial, after reading > >>>> post after post of $30,000+ and $18,000 and similarly high figures > >>>> for lab microwaves, I really feel the need to set the record > >>>> straight. Depending on the usage requirements, we have laboratory > >>>> microwaves as low as $1749 for a "bare bones" model for simple > >>>> operations, to mid-priced units, to under $11,000 for a vacuum > >>>> equipped microwave processor capable of the +/- 0.5 degree C > >>>> temperature control necessary for tissue processing. > >>>> > >>>> (I can feel the heat already!) > >>>> > >>>> There are many compelling reasons to replace a kitchen microwave > >>>> with a lab model; feel free to download, read, and even share with > >>>> colleagues our Microwave Companion at > >>>> > >>>> http://www.ebsciences.com/pdf/EBS_MW_COMPANION.pdf > >>>> > >>>> Best regards, and see you at NSH, > >>>> > >>>> Phil McArdle > >>>> > >>>> -- > >>>> Phil McArdle > >>>> Microwave Product Manager > >>>> > >>>> Energy Beam Sciences, Inc. > >>>> 29-B Kripes Rd. > >>>> East Granby, CT 06026 > >>>> > >>>> Tel: 800.992.9037 x 341 > >>>> Mobile: 860.597.6796 > >>>> Fax: 860.653.0422 > >>>> > >>>> pmcardle@ebsciences.com > >>>> www.ebsciences.com > >>>> > >>>> Kathleen Boozer wrote: > >>>>> What is the best microwave for a small lab using it only for > >>>>> heating Bouin's and Silver Nitrate for special stains? I just > >>>>> can't believe I would have to spend $30,000+ or slow down and use a > >>>>> waterbath. > >>>>> > >>>>> > >>>>> _______________________________________________ > >>>>> Histonet mailing list > >>>>> Histonet@lists.utsouthwestern.edu > >>>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >>>> > >>>> > >>>> > >>>> > >>>> I skate to where the puck is going to be, not to where it's been. > >>>> - Wayne Gretsky > >>>> > >>>> You must be the change you want to see in the world. > >>>> - Mahatma Gandhi > >>>> > >>>> NOTE: This message, together with any attachments, is intended only > >>>> for the use of the individual or entity to which it is addressed and > >>>> may contain information that is legally privileged, confidential and > >>>> exempt from disclosure. If you are not the intended recipient, > >>>> however, there's not a lot I can do about it, and it was probably my > >>>> mistake anyway. So please do the right thing and make this e-mail go > >>>> away. Thank you. > >>>> > >>>> _______________________________________________ > >>>> Histonet mailing list > >>>> Histonet@lists.utsouthwestern.edu > >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >>> > >>> > >>> _______________________________________________ > >>> Histonet mailing list > >>> Histonet@lists.utsouthwestern.edu > >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > >> > >> -- > >> Phil McArdle > >> Microwave Product Manager > >> > >> Energy Beam Sciences, Inc. > >> 29-B Kripes Rd. > >> East Granby, CT 06026 > >> > >> Tel: 800.992.9037 x 341 > >> Mobile: 860.597.6796 > >> Fax: 860.653.0422 > >> > >> pmcardle@ebsciences.com > >> www.ebsciences.com > >> > >> I skate to where the puck is going to be, not to where it's been. > >> - Wayne Gretsky > >> > >> You must be the change you want to see in the world. > >> - Mahatma Gandhi > >> > >> NOTE: This message, together with any attachments, is intended only > >> for the use of the individual or entity to which it is addressed and > >> may contain information that is legally privileged, confidential and > >> exempt from disclosure. If you are not the intended recipient, > >> however, there's not a lot I can do about it, and it was probably my > >> mistake anyway. So please do the right thing and make this e-mail go > >> away. Thank you. > > > > I skate to where the puck is going to be, not to where it's been. > - Wayne Gretsky > > You must be the change you want to see in the world. > - Mahatma Gandhi > > NOTE: This message, together with any attachments, is intended only for > the use of the individual or entity to which it is addressed and may > contain information that is legally privileged, confidential and exempt > from disclosure. If you are not the intended recipient, however, there's > not a lot I can do about it, and it was probably my mistake anyway. So > please do the right thing and make this e-mail go away. Thank you. > > ------------------------------ > > Message: 10 > Date: Wed, 5 Sep 2007 13:01:21 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Xylene > To: themagoos@rushmore.com, histonet@lists.utsouthwestern.edu > Message-ID: <329330.36844.qm@web61219.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Tissues usually become brittle, which difficulties sectioning. > Ren? J. > > themagoos wrote: > Can somebody tell me if there are any effects on tissue if > there is prolonged processing time in xylene? > > Jason McGough HT(ASCP) > Clinical Laboratory of the Black Hills > Account Representative - Anatomic Pathology > 2805 5th Street > Rapid City, SD 57701 > 605-343-2267 > jmcgough@clinlab.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > --------------------------------- > Boardwalk for $500? In 2007? Ha! > Play Monopoly Here and Now (it's updated for today's economy) at Yahoo! > Games. > > ------------------------------ > > Message: 11 > Date: Wed, 5 Sep 2007 14:09:02 -0600 > From: "Liz Chlipala" > Subject: RE: [Histonet] IHC anti-cleaved caspase 3 > To: "Cheryl Cross" , "Thomas Pier" > > Cc: carl.hobbs@kcl.ac.uk, histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset="windows-1250" > > I like the cell signaling antibody also, I tried biocare's but did not > have > much success with it. The cell signaling antibody also works with pronase > digestion, as well as the EDTA pH9 HIER. We have even used it on bone > sections with the pronase digestion. Works in multiple species, I have > used it > on human, rat, mouse, guinea pig, porcine and canine. Our protocol is > similar > to Cheryl's. It’s a bit pricy as antibodies go, but I feel its > worth it. > Good lot to lot consistency. We have probably gone through about 5 > different > lots, with not changing the protocol at all. I have a written protocol if > anyone is interested. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Manager > Premier Laboratory, LLC > P.O. Box 18592 > Boulder, CO 80308 > phone (303) 735-5001 > fax (303) 735-3540 > liz@premierlab.com > www.premierlab.com > > Ship to Address: > > Premier Laboratory, LLC > University of Colorado at Boulder > MCDB, Room A3B40 > Boulder, CO 80309 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Cross Sent: > Wednesday, > September 05, 2007 2:01 PM To: Thomas Pier Cc: carl.hobbs@kcl.ac.uk; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] IHC anti-cleaved caspase 3 > > I will second the Cell Signaling antibody - we have tried antibodies from > Promega (which gave tons of background staining)...then via this site i > was > pointed to Cell Signaling's monoclonal which we tried; the polyclonal > actually > has been working very well with minimal background (we were getting lots > of > respiratory epithelium and endothelium lighting up in control animals). I > have > been using it in mice with this protocol: > > EDTA with PASCAL > primary antibody 60 minutes, dilution 1:125 Rabbit envision DAB plus > > Hope this helps! > > Cheryl Cross, DVM, Dipl. ACVP > Researcher > University Corporation for Atmospheric Research College of Veterinary > Medicine > University of Tennessee Department of Pathology 2407 River Drive, Room > A201 > Knoxville, TN 37996-4542 > (423) 967-2724 fax: 865-974-5616 ccross@ucar.edu > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.5.485 / Virus Database: 269.13.5/990 - Release Date: 9/4/2007 > 10:36 > PM > > No virus found in this outgoing message. > Checked by AVG Free Edition. > Version: 7.5.485 / Virus Database: 269.13.5/990 - Release Date: 9/4/2007 > 10:36 > PM > > ------------------------------ > > Message: 12 > Date: Wed, 05 Sep 2007 16:13:36 -0400 > From: Sarah Clatterbuck Soper > Subject: [Histonet] Freezing mouse testes for frozen sections > To: histonet@lists.utsouthwestern.edu > Message-ID: <46DF0DF0.2030306@ciwemb.edu> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Hi all, > > I've started attempting to section unfixed frozen mouse testes in order > to placate a specific antibody we have to use. I am new to frozen > sections and I'm having trouble with the testes cracking when I freeze > them. I've tried both freezing in isopentane cooled on liquid nitrogen > and an acetone/dry ice slurry. Either way the testes crack, usually one > big crack end to end. Doesn't seem to be as much of a problem with our > mutant testes, which are about 1/3 the size of wild-type. I wish I > could just trim the wild-type down to a smaller size, but obviously > that's not an option! > > Any recommendations? > > Thanks so much! > > Sarah > > ------------------------------ > > Message: 13 > Date: Wed, 5 Sep 2007 15:49:48 -0500 > From: "Mike Pence" > Subject: [Histonet] Olympus BX40 > To: > Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C701@IS-E2K3.grhs.net> > Content-Type: text/plain; charset="us-ascii" > > Need some help, > > I am looking for a 60x objective for an Olympus microscope BX40. > Would anyone know where I might get a used one or if they even make one > this size for this scope? > > Thanks, > Mike > > ------------------------------ > > Message: 14 > Date: Wed, 5 Sep 2007 16:58:12 -0400 > From: "Yu, Jian" > Subject: [Histonet] Look for a used stereoscope > To: > Message-ID: > <7E0A77BFEB9A1E47A63F978E7116821F07986D6D@1upmc-msx11.acct.upmchs.net> > Content-Type: text/plain; charset="us-ascii" > > Does anyone know a good place to get a used stereoscope? I plan to use > it to examine intestinal tumors in mice. > > Thanks a lot for your information. > > ******************************************************************* > Jian Yu, Ph.D. > University of Pittsburgh Cancer Institute > Hillman Cancer Center Research Pavilion > Office Suite 2.26h > 5117 Centre Avenue, Pittsburgh, PA 15213 > ******************************************************************* > > ------------------------------ > > Message: 15 > Date: Thu, 6 Sep 2007 09:44:45 +1000 > From: "Tony Henwood" > Subject: RE: [Histonet] RDO decal > To: "Rene J Buesa" , "RENEE FISHER" > , > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > There is also the issue of EDTA, which is common in many RDO formulations. > Checking one MSDS for EDTA reveals: > > Ecological Information > Environmental Fate: > When released into the soil, this material is expected to leach into > groundwater. When released into the soil, this material may biodegrade > to a > moderate extent. When released into the soil, this material is not > expected > to evaporate significantly. When released into water, this material is > not > expected to evaporate significantly. This material is not expected to > significantly bioaccumulate. When released into the air, this material is > expected to be readily degraded by photolysis. Environmental Toxicity: > This material is not expected to be toxic to aquatic life. The > LC50/96-hour > values for fish are over 100 mg/l. > > So neutralisation may not be required. > If anyone has info to the contrary please advise. > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) > Laboratory Manager & Senior Scientist > The Children's Hospital at Westmead, > Locked Bag 4001, Westmead, 2145, AUSTRALIA. > Tel: 612 9845 3306 > Fax: 612 9845 3318 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: > Thursday, 6 > September 2007 1:07 AM To: RENEE FISHER; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] RDO decal > > Ren?e: > I have not heard of neutralizing RDO, but it would make sense if you > want to > be "gentle on your sewer system" BUT prepare to a large emission of carbon > dioxide when attempting to neutralize it with baking soda. RDO + baking > soda > (or sodium bicarbonate) will produce water, salt with the acid in RDO > (probably sodium chloride or common salt), and carbon dioxide in > stoichiometrical amounts (1 CO2 per every 1 NaCl). Therefore that > neutralization has to take place in a fumes hood, and you will have to > decide > which is worst: delivering acid to the sewer system, or carbon dioxide > (the > Greenhouse gas per excellence) to the atmosphere. It will be "your call". > Ren? J. > > RENEE FISHER wrote: > Has anyone heard of neutralizing RDO with baking soda to P.H. 7.0. Our > Histo > lab had a hazardous waste assessment, and the consultant suggested we not > throw the RDO down the drain but that we either collect it for waste > removal > or neutralize it with baking soda to p.h. 7.0. I have not heard of doing > this > and do not know of any procedure for it, everyone, anyone's help will be > greatly appreciated. > > Thanks, > Renee' > > _______________________________________________________________________________________ > > This email may contain confidential protected health information and/or > proprietary information belonging to the sender that is legally privileged > under local, state, or federal law. This information is intended only for > the > use of the individual or individuals who have received this. The > authorized > recipient of this information is prohibited from disclosing this > information > to any other party unless required to do so by law. If you are not the > intended recipient, you are hereby notified that any disclosure, copying, > distribution, or action taken in reliance on the contents of this email > is > strictly prohibited. If you have received this email in error, please > notify > the sender immediately to arrange for the disposal of this information. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > --------------------------------- > Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: mail, > news, > photos & more. _______________________________________________ Histonet > mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************* > This email and any files transmitted with it are confidential and intended > solely for the use of the individual or entity to whom they are addressed. > If > you are not the intended recipient, please delete it and notify the > sender. > > Views expressed in this message and any attachments are those of the > individual sender, and are not necessarily the views of The Children's > Hospital at Westmead > > This note also confirms that this email message has been > virus scanned and although no computer viruses were detected, The > Childrens > Hospital at Westmead accepts no liability for any consequential damage > resulting from email containing computer viruses. ********************************************************************** > > ------------------------------ > > Message: 16 > Date: Thu, 6 Sep 2007 11:23:00 +0900 > From: ChoiUl Soo > Subject: [Histonet] PTH antibody reacted with dog tissue... > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="ks_c_5601-1987" > > Hi Histonetters, > > I am interested in anti PTH antibody reacted with dog tissue. > If anyone has successful experience with any PTH antibody with dog tissue, > please tell me one. > > I have searched through the internet, and results came back with abcam and > SantaCruz PTH antibodies predicted to react with them. But they are not > sure > on it, just predicted on the basis of sequence homology. > (abcam say 94% homology with human) I don't have good exprience with Santa > Cruz, and never used one by Abcam. Should I rely on it, or find another > one? > > Let me hear your experience. > > I would appreciate your advice or comment on this. > > Thank you~. > > Ul Soo Choi, DVM, PhDKRF priority zoonotic disease research institute, > College > of Veterinary Medicine, Seoul National University, Shilim9 dong, Gwanakgu, > Seoul, Korea 151-742Tel. 82-02-880-8688 Mobile. 82-016-9228-8634Fax. > 82-02-880- > 8662 > > _________________________________________________________________ > ???? ?????? ????, Windows Live Space! > http://www.spaces.live.com > > ------------------------------ > > Message: 17 > Date: Thu, 6 Sep 2007 01:57:04 -0400 > From: "Michelle McCoy" > Subject: [Histonet] Floater sources > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <25355ef80709052257i32794198gd5a64b94758cc1ea@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > I was reading some of the old archived messages on floaters, and being > somewhat new to the field of histotechnology was curious about how > floaters > can be attributed to a particular tech who performed work on the block. > For > example, couldn't other sources of contamination be from the automatic > stainer, processor carryover (cassette not completely closed/or if closed, > friable tissue through the slats eg if sponge not used on larger > specimen). > In some previous labs I've worked in- floaters were quickly attributed to > the cutter, embedder or grosser and might result in a "write up". If the > floater is seen in the block how can you differentiate if it came from the > grosser carryover/embedder carryover/processing carryover/unsigned > re-embedder/or even possibly even client carry over between patients or > different specimen types of the same patient. > And if it is not in the block --differentiating between the > cutter/automatic > stainer (I've seen specks of tissue debris in automatic stainers/ sections > falling off the slides and into the reagents etc). Obviously all should be > done to minimize the factors, but I'm just not clear how a single source > is > pinpointed and potentially blamed for the event (depending on the lab > policy). Thanks for any ideas on this. > > ------------------------------ > > Message: 18 > Date: Thu, 6 Sep 2007 08:07:38 +0100 > From: "Kemlo Rogerson" > Subject: RE: [Histonet] Xylene > To: , > Message-ID: > <86ADE4EB583CE64799A9924684A0FBBF0222EC6C@wahtntex2.waht.swest.nhs.uk> > Content-Type: text/plain; charset="us-ascii" > > Can somebody tell me if there are any effects on tissue if there is > prolonged processing time in xylene? > > Jason McGough HT(ASCP) > Clinical Laboratory of the Black Hills > Account Representative - Anatomic Pathology > 2805 5th Street > Rapid City, SD 57701 > 605-343-2267 > jmcgough@clinlab.com > > Classically it is said to harden, plus more lipids could be removed. > Personally I'm equivocal about that thought; if properly fixed prior to > processing I would have thought the hardening effects of xylene were > much less than that of the coagulant fixative ethanol. If you do have > hard tissue then there is a restorative fluid one can use which I think > has oil of cedarwood in it but I don't know the formula off hand. I know > it works cos when I was a pup I used it sometimes. > > Kemlo Rogerson > Pathology Manager > DD 01934 647057 or extension 3311 > Mob 07749 754194; Pager 07659 597107; > > Sunshine is delicious, rain is refreshing, wind braces us up, snow is > exhilarating; there is really no such thing as bad weather, only > different kinds of good weather. --John Ruskin > > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. > Please inform me that this message has gone astray before deleting it. > Thank you for your co-operation > > ------------------------------ > > Message: 19 > Date: Thu, 6 Sep 2007 08:17:36 +0100 > From: "Kemlo Rogerson" > Subject: RE: [Histonet] Floater sources > To: "Michelle McCoy" , > > Message-ID: > <86ADE4EB583CE64799A9924684A0FBBF0222EC6D@wahtntex2.waht.swest.nhs.uk> > Content-Type: text/plain; charset="us-ascii" > > I was reading some of the old archived messages on floaters, and being > somewhat new to the field of histotechnology was curious about how > floaters can be attributed to a particular tech who performed work on > the block. For example, couldn't other sources of contamination be from > the automatic stainer, processor carryover (cassette not completely > closed/or if closed, friable tissue through the slats eg if sponge not > used on larger specimen). In some previous labs I've worked in- floaters > were quickly attributed to the cutter, embedder or grosser and might > result in a "write up". If the floater is seen in the block how can you > differentiate if it came from the grosser carryover/embedder > carryover/processing carryover/unsigned re-embedder/or even possibly > even client carry over between patients or different specimen types of > the same patient. And if it is not in the block --differentiating > between the cutter/automatic stainer (I've seen specks of tissue debris > in automatic stainers/ sections falling off the slides and into the > reagents etc). Obviously all should be done to minimize the factors, but > I'm just not clear how a single source is pinpointed and potentially > blamed for the event (depending on the lab policy). Thanks for any ideas > on this. > > You are exactly correct, floaters can be attributed to a variety of > causes, processing machines that aren't regularly changed, transfer on > the cutting up forceps, on the waterbath and on the forceps of the > embedder. If the floater was from a block that was cut, embedded, cut up > before the section with the floater then the culprit is obvious. If the > section was cut by someone who didn't cut the block from which the > floater floated, then the culprit is the embedder, machine,or cutter up. > Realisticaly I would have thought that most floaters would be from the > embedder, cutter up, then sectioner as the latter has the greater > likelihood of seeing the error of his/ her ways. Waxy forceps of messy > forceps, in my experience are the usual culprit but in some instances, > necrotic tumours can shed cells in the processor and even the stainer. > > Floaters are usually obvious as they tend to be at a different level > than the tissue section and I'm afraid they are a fact of life. You can > reduce the incidence but sadly never eradicate them. > > Kemlo Rogerson > Pathology Manager > DD 01934 647057 or extension 3311 > Mob 07749 754194; Pager 07659 597107; > > Sunshine is delicious, rain is refreshing, wind braces us up, snow is > exhilarating; there is really no such thing as bad weather, only > different kinds of good weather. --John Ruskin > > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. > Please inform me that this message has gone astray before deleting it. > Thank you for your co-operation > > ------------------------------ > > Message: 20 > Date: Thu, 6 Sep 2007 04:18:04 -0400 > From: "Victoria Baker" > Subject: [Histonet] Fwd: Users of Lab Vision autostainer > To: histonet > Message-ID: > <4f016b690709060118t532d22c1if250116e0a6c9af1@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > ---------- Forwarded message ---------- > From: Victoria Baker > Date: Sep 5, 2007 9:02 AM > Subject: Users of Lab Vision autostainer > To: Histo Net list server > > Hi > > I'm a new user of the Lab Vision autostainer and I'm looking to see if > I can find users in Histo-land that have experience with it. The > facility only works with human tissue and all of the antibodies are > for dx purposes. > > My key questions are as follows: > How many antibodies is your lab running? > How many users do you allow? > How many people do you allow programming rights and at what level of > supervision are they? > > How many of these antibodies are from Lab Vision? > a) are they concentrates or pre-dilutes? > b) for HIER are you using their PT modules/procedures or > your own equipment (microwave, steamer, pressure cooker etc) and in > house designed protocols for retrieval? > c) for digestion do you only use their Pro-K or have you > designed your own in-house methods using other reagents for digestion? > d) do you put your controls on the same slide as the patient? > e) are your controls in-house or commercial? > f) do you have more than one stainer hooked up to one computer > system? > > What Version of software do you currently have on your system? > > Any feed back would be very helpful. > > Thanks in advance. > > Vikki Baker > Interim Histology Manager > Mission Hospital System > Asheville, NC > > ------------------------------ > > Message: 21 > Date: Thu, 6 Sep 2007 02:28:57 -0600 > From: "Joseph Kapler" > Subject: RE: [Histonet] Xylene > To: "Rene J Buesa" , , > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Tissues processed in Xylene (especially over long periods of time) the > tissue becomes very brittle. > > Hope that is the response you were looking for. > > Joseph "DarkWolfe" Kapler > I'm an outsider outside of everything > I'm an outsider outside of everything > I'm an outsider outside of everything > Everything you know. Everything you know > It disturbs me so > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J > Buesa > Sent: Wednesday, September 05, 2007 14:01 > To: themagoos@rushmore.com; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Xylene > > Tissues usually become brittle, which difficulties sectioning. > Ren? J. > > themagoos wrote: > Can somebody tell me if there are any effects on tissue if > there is prolonged processing time in xylene? > > Jason McGough HT(ASCP) > Clinical Laboratory of the Black Hills > Account Representative - Anatomic Pathology > 2805 5th Street > Rapid City, SD 57701 > 605-343-2267 > jmcgough@clinlab.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > --------------------------------- > Boardwalk for $500? In 2007? Ha! > Play Monopoly Here and Now (it's updated for today's economy) at Yahoo! > Games. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.5.485 / Virus Database: 269.13.5/988 - Release Date: 9/4/2007 > 09:14 > > No virus found in this outgoing message. > Checked by AVG Free Edition. > Version: 7.5.485 / Virus Database: 269.13.5/988 - Release Date: 9/4/2007 > 09:14 > > ------------------------------ > > Message: 22 > Date: Thu, 6 Sep 2007 09:59:13 +0100 > From: Malam Jacqueline > Subject: [Histonet] Unsubscribe > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain > > Please would you unsubscribe me as I am retiring tomorrow - thanks > > Jacqui malam > Lancaster > uk > > DISCLAIMER: This e-mail is confidential and privileged. If you are not the > intended recipient please accept our apologies; please do not disclose, > copy > or distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. Please > inform postmaster@rli.mbht.nhs.uk that this message has gone astray before > deleting it. Comments or opinions expressed in this email are those of > their respective contributors only. The views expressed do not represent > the > views of the Trust, its management or employees. University Hospitals of > Morecambe Bay NHS Trust is not responsible and disclaims any and all > liability for the content of comments written within.Thank you for your > co-operation. > > ------------------------------ > > Message: 23 > Date: Thu, 6 Sep 2007 06:31:58 -0500 > From: "Joe Nocito" > Subject: Re: [Histonet] Floater sources > To: "Michelle McCoy" , > > Message-ID: <004201c7f079$8f5aa560$0202a8c0@yourxhtr8hvc4p> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > Michelle, > first. welcome to world of histology. > Let's begin at the grossing table- all grossers are trained to wipe off > the > table after each case- but that doesn't mean floaters can't happen, but > the > incidence is low > third- embedders should have been taught to clean the embedding area after > each case and at the end of the day, but this is a good source for > contamination. You can see this is the block. > fourth- the cutters should wipe off their waterbaths after each block. > This > probably is the most likely source of contamination. I've inspected some > labs where one waterbath was just covered with previous ribbons, attached > to > the side and floating on the waterbath. > fifth- in my experience, it is highly unlikely that floaters came from the > stainer just by the shear movement of the slides and water. > > The bottom line is that everyone needs to be neat and clean and be > meticulous. Remember, cleanliness is next to Godliness. > I had a testicular seminoma where no matter what we did, floaters still > were > on cervical bxs and endometrial bxs. The medical and I decided that we > would handle cases like these separately. They were processed, embedded, > cut > and stained separately. Once the case was completed, we changed all the > solutions on the tissue processor and stainer. Who ever cut the blocks had > to dismantle their knife holder and microtome to clean it thoroughly. A > pain > in the butt, but was a necessary evil. > > I hope this helped a little. Good luck. > > Joe The Toe > ----- Original Message ----- > From: "Michelle McCoy" > To: > Sent: Thursday, September 06, 2007 12:57 AM > Subject: [Histonet] Floater sources > > >I was reading some of the old archived messages on floaters, and being > > somewhat new to the field of histotechnology was curious about how > > floaters > > can be attributed to a particular tech who performed work on the block. > > For > > example, couldn't other sources of contamination be from the automatic > > stainer, processor carryover (cassette not completely closed/or if > > closed, > > friable tissue through the slats eg if sponge not used on larger > > specimen). > > In some previous labs I've worked in- floaters were quickly attributed > > to > > the cutter, embedder or grosser and might result in a "write up". If the > > floater is seen in the block how can you differentiate if it came from > > the > > grosser carryover/embedder carryover/processing carryover/unsigned > > re-embedder/or even possibly even client carry over between patients or > > different specimen types of the same patient. > > And if it is not in the block --differentiating between the > > cutter/automatic > > stainer (I've seen specks of tissue debris in automatic stainers/ > > sections > > falling off the slides and into the reagents etc). Obviously all should > > be > > done to minimize the factors, but I'm just not clear how a single source > > is > > pinpointed and potentially blamed for the event (depending on the lab > > policy). Thanks for any ideas on this. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 46, Issue 6 > *************************************** ------- End of Original Message ------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cdemarinis <@t> SARATOGACARE.ORG Fri Sep 7 06:15:07 2007 From: cdemarinis <@t> SARATOGACARE.ORG (Demarinis, Carolyn) Date: Fri Sep 7 06:15:21 2007 Subject: [Histonet] FW: Emailing: junk6024.jpg Message-ID: We are getting pink blobs on our H&E and special stain slides with no nuclei. Can anyone tell me what is causing this? Thanks. From BMolinari <@t> heart.thi.tmc.edu Fri Sep 7 06:42:33 2007 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri Sep 7 06:42:45 2007 Subject: [Histonet] Job Opening (Columbia SC) In-Reply-To: Message-ID: WOW that is some pond!!! Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Burton, Mark Sent: Thursday, September 06, 2007 12:38 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Job Opening (Columbia SC) Make sure you click the hybrid button on the Google map. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Thursday, September 06, 2007 2:15 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Job Opening (Columbia SC) For all of you that found the "pond" amusing here you go. :) http://maps.google.com/?ie=UTF8&ll=34.099354,-80.964872&spn=0.00239,0.003648 &t=k&z=18&om=1 Bring your fishing pole! Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Thursday, September 06, 2007 11:56 AM To: Douglas D Deltour; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Job Opening (Columbia SC) Is the pond lighted? Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Thursday, September 06, 2007 9:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Job Opening (Columbia SC) Professional Pathology Services, PC is one of the regions fastest growing Pathology laboratories located in sunny Columbia South Carolina. Our state of the art histology laboratory has a wall of windows with a scenic view of our own pond. Come join our fun and exciting team of healthcare professionals. We are seeking a full-time certified histology technician ASCP certified w/1-5 years of experience in Histology New Grads welcome to apply This position is for the hours of 9:30PM-6:00AM NO RECRUITERS PLEASE Send r?sum? to Doug@ppspath.com www.ppspath.com Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sheila_adey <@t> hotmail.com Fri Sep 7 07:37:25 2007 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Fri Sep 7 07:37:48 2007 Subject: [Histonet] IHC negatives staining positive???? Message-ID: Hello Netters, we use the Biogenex i6000 and have recently been having a problem with positive staining on our negatives. Any ideas? It is happening with the HEIR and enzymatic retrievals. Thanks in advance Sheila Adey HT MLT Port Huron Hospital Michigan _________________________________________________________________ Former Police Officer Paul Gillespie’s TAKE BACK THE INTERNET tips and tricks, watch the video now http://safety.sympatico.msn.ca/ From GDawson <@t> dynacaremilwaukee.com Fri Sep 7 07:45:41 2007 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Fri Sep 7 07:46:03 2007 Subject: [Histonet] IHC staining weaker in... In-Reply-To: <407F05A128805F4C879A33DBA32E618E01895031@TRFT-EX01.xRothGen.nhs.uk> Message-ID: To test if this is a fixation problem, cut a slide, deparaffinize it, place in formalin for 10 minutes, rinse, and then stain as usual (same epitope retirieval, same procedure). If the tissue stains uniformly after this procedure...it WAS a fixation problem. Glen Dawson IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Friday, September 07, 2007 5:45 AM To: Joe Nocito; Department of Pathology; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC staining weaker in... Everyone is shouting fixation problem, but is this something we see? I don't. The only block that regularly shows something like this is a lymph node that has been put in intact, but there, there is the additional autolysis, which the questioner did not mention. In addition, I gather from the question, that this is a general fault. WE perhaps need more information perhaps. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: 06 September 2007 21:35 To: Marshall Terry Dr, Consultant Histopathologist; Department of Pathology; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC staining weaker in... sounds like a fixation problem. Joe ----- Original Message ----- From: "Marshall Terry Dr, Consultant Histopathologist" To: "Department of Pathology" ; Sent: Thursday, September 06, 2007 11:14 AM Subject: RE: [Histonet] IHC staining weaker in... I thought I had seen every horror possible in a section, but that seems a new one to me! Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Department of Pathology Sent: 06 September 2007 16:48 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC staining weaker in... Dear Participants, our diagnostic pathology lab has a problem. The paraffin sections are stained weakly at the center but better at the periphery of the sections. What could be the reason.??... thanks a lot.. Dr Nasuhi Engin Aydin, malatya Inonu University Hospital, Turkey 44365. ---------- Original Message ----------- From: histonet-request@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Thu, 6 Sep 2007 14:51:24 +0300 (EEST) Subject: Histonet Digest, Vol 46, Issue 6 > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > Today's Topics: > > 1. Xylene (themagoos) > 2. RE:The great apoptosis stains debate (TUNEL, Caspases, etc) > (Melissa Gonzalez) > 3. Re: RDO decalcifier (Robert Richmond) > 4. Re: IHC anti-cleaved caspase 3 (Carl Hobbs) > 5. avian sperm cell topography (melissah rowe) > 6. Re: IHC anti-cleaved caspase 3 (Thomas Pier) > 7. Re: IHC anti-cleaved caspase 3 (Cheryl Cross) > 8. Fwd: Users of Lab Vision autostainer (Victoria Baker) > 9. Re: Microwaves VENDOR RESPONSE (Phil McArdle) > 10. Re: Xylene (Rene J Buesa) > 11. RE: IHC anti-cleaved caspase 3 (Liz Chlipala) > 12. Freezing mouse testes for frozen sections > (Sarah Clatterbuck Soper) > 13. Olympus BX40 (Mike Pence) > 14. Look for a used stereoscope (Yu, Jian) > 15. RE: RDO decal (Tony Henwood) > 16. PTH antibody reacted with dog tissue... (ChoiUl Soo) > 17. Floater sources (Michelle McCoy) > 18. RE: Xylene (Kemlo Rogerson) > 19. RE: Floater sources (Kemlo Rogerson) > 20. Fwd: Users of Lab Vision autostainer (Victoria Baker) > 21. RE: Xylene (Joseph Kapler) > 22. Unsubscribe (Malam Jacqueline) > 23. Re: Floater sources (Joe Nocito) > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 05 Sep 2007 10:21:51 -0700 > From: "themagoos" > Subject: [Histonet] Xylene > To: histonet@lists.utsouthwestern.edu > Message-ID: <46dee5af.14b.450a.1030817715@rushmore.com> > Content-Type: text/plain; charset="iso-8859-1" > > Can somebody tell me if there are any effects on tissue if > there is prolonged processing time in xylene? > > Jason McGough HT(ASCP) > Clinical Laboratory of the Black Hills > Account Representative - Anatomic Pathology > 2805 5th Street > Rapid City, SD 57701 > 605-343-2267 > jmcgough@clinlab.com > > ------------------------------ > > Message: 2 > Date: Wed, 5 Sep 2007 11:30:38 -0700 > From: "Melissa Gonzalez" > Subject: [Histonet] RE:The great apoptosis stains debate (TUNEL, > Caspases, etc) > To: > Message-ID: > <2884B897182A1D438C7BA24B9A8F94A20E701D@hqsvr01mail.cgi.com> > Content-Type: text/plain; charset="iso-8859-1" > > Hi all, > I have been around this mess a few years back, I gave up on TUNEL long ago > for > the various reasons mentioned on the list recently. > > Per investigators requests, I have tried several ways to demonstrate cell > death, however methods such as PI or Annexin staining are more suitable > for > whole cells, not tissue sections. I have also not had any luck staining > for > Cytochrome C. > > We routinely use R&D Systems rabbit anti human/mouse active Caspase3 using > a > high pH (EDTA) based Ag retrieval in a steamer (enzymes do not work). > Works > like a charm. > > Any other suggestions? I know this topic has been thrown around a lot, but > these discussions after all, help us get our jobs done more proficiently. > > Melissa > > Melissa A. Gonz?lez Edick > R&D, Cell Genesys Inc. > 500 Forbes Blvd > South San Francisco, CA 94080 > p(650) 266-3168 > f (650) 266-3080 > > "It's not enough to believe what you see, you must also understand what > you > see." -Leonardo Da Vinci > ------------------------------ > > Message: 6 > Date: Tue, 4 Sep 2007 15:18:39 -0400 > From: Cheryl Cross > Subject: Re: [Histonet] TUNEL, Formalin, DNA strand breaks > To: JR R > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <4C1E074D-064A-45D1-BD32-13D7E2222B26@aol.com> > Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed > > Hi all - > > I have been round and round with this issue myself; I was basically > told by people who've attempted it that TUNEL on FFPE sections is not > specific enough due to the formalin issue. > > If you are trying to nail apoptosis, what about anti-active > caspase-3? mind you, that staining can be a bit of a booger too, but > it should be more sensitive and specific for apoptosis (no references > to offer, i'm just repeating what i have been told). > > Cheryl Cross, DVM, Dipl. ACVP > Researcher > University Corporation for Atmospheric Research > College of Veterinary Medicine > University of Tennessee Department of Pathology > 2407 River Drive, Room A201 > Knoxville, TN 37996-4542 > (423) 967-2724 > fax: 865-974-5616 > ccross@ucar.edu > ------------------------------ > > Message: 5 > Hi Ray, > > Sheesh-- I read (and then lost) the paper maybe 5-7 years ago, back when I > was > tearing my hair out over high background, or false positives for TUNEL > stain > in formalin fixed, paraffin embedded arterial sections. I can > troubleshoot > any immunostain, but the TUNEL and ISEL assays bedeviled me. > > The paper was titled something to the effect of "formaldehyde causes > single > and double stranded DNA breaks," and was pretty emphatic. I'll look for > the article. > > I think the assay could work in whole cells for flow, or maybe in frozen > tissue. My hunch at this point is that this is one of those rare cases > where > lots and lots of peer reviewed articles are just plain wrong. > > Hey, if someone has a good protocol, I'd be willing to try it out, and I > would > be delighted if I turn out to be mistaken. For now, I think TUNEL is the > assay of the beast. > > Oh, here is a thought--say you are looking at a 5 micron section through a > nucleus. The microtome blade pretty much had to create a lot of double > stranded DNA breaks, no? > > Jerry L. Ricks > Research Scientist > U.W. Medicine at South Lake Union > 815 Mercer Street > Seattle, WA 98109 > (206)-685-7190 > > From: koellingr@comcast.netTo: rosenfeldtek@hotmail.com; > histonet@lists.utsouthwestern.eduSubject: RE: [Histonet] TUNELDate: Fri, > 31 > Aug 2007 23:17:03 +0000 > > Jerry, > Could you expand on or give references to formalin causing DNA strand > breaks? > Double strand breaks, single strand, blunt end, overhanging? My pile of > papers and having done TUNEL for years says that formalin fixation is a > very > good technique for TUNEL and many peer-reviewed articles in which TUNEL is > used as a technique, use formalin fixation and how can that be if formalin > is > causing strand breaks? In fact one paper I'm looking at says that > extended (5- > 7 weeks in formalin) fixation causes loss of TUNEL signal. If formalin is > causing breaks, you would assume that TUNEL pos signals would increase > with > extended formalin fixation. Even the use of the monoclonal antibody > F7-26, > for single stranded DNA, touts formalin fixation for their claims of > discriminating apoptosis from necrosis. > > The question asks about extended alcohol fixation but your answer is > possibly > a lot of false positives because formalin causes DNA breaks. Does this > imply > that alcohol won't? Have done a lot of TUNEL on alcohol fixed samples. > True > I couldn't pretreat them and handle them they way I would handle FFPE > tissue. > Also true that we could argue specificity and ability or not to > discriminate > apoptosis from necrosis for quite a while. But if formalin itself is > causing > the breaks in DNA, I and a lot of people are in big trouble with our > science > projects and experiments. Also I can't envision why 2 cells are showing > TUNEL > positivity while 2 of the same type of cells right next to them (and > getting > the same formalin fix), are absolutely clean and there is no background? > > Thanks for any information you can provide. > > Ray Koelling > PhenoPath Laboratories > Seattle, WA > > ------------------------------ > > Message: 3 > Date: Wed, 5 Sep 2007 14:56:14 -0400 > From: "Robert Richmond" > Subject: [Histonet] Re: RDO decalcifier > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > I don't see how your hazmats people can tell you what to do to dispose > of RDO, since nobody has any idea what's in it. It's a murky yellow > liquid that continuously throws a black sediment that has to be > filtered out if you're to see your specimen in it. It's sort of the > ultimate secret formula, and I've always wondered why it's so popular > - I've had to use it many different places in my travels. > > I'd suggest either an ordinary proprietary decalcifier that's a clear > liquid, probably hydrochloric acid - or else save money and dilute > your own hydrochloric acid. You can re-use it for a while. When the > time comes to dispose of it, it can be safely diluted with a lot of > water and put down the drain. > > Remember - it's been stressed on this list many times - that "Decal" > is a brand name still in use, and that other brands of decalcifying > solution should not be called Decal. > > Bob Richmond > Samurai Pathologist (never a decalcified Carmelite) > Knoxville TN > > ------------------------------ > > Message: 4 > Date: Wed, 5 Sep 2007 20:29:36 +0100 > From: "Carl Hobbs" > Subject: Re: [Histonet] IHC anti-cleaved caspase 3 > To: "Histonet" > Message-ID: <001801c7eff3$18894850$4101a8c0@carlba65530bda> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > Hi. > > Recent Cleaved caspase 3 posts have not mentioned the antibody details: I > would be grateful for the source of these Abs. > Carl > > ------------------------------ > > Message: 5 > Date: Wed, 5 Sep 2007 14:36:50 -0500 > From: melissah rowe > Subject: [Histonet] avian sperm cell topography > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed > > Hi, > > I am a PhD student working on a behavioral ecology project on > Australian fairy-wrens. I am currently trying to find out if I can > determine the dimensions of discrete sections of individual sperm > cells, i.e. acrosome length, nucleus length, midpiece length and tail > length using the material I currently have available. I have prepared > slide smears of sperm that has been previously stained with an eosin- > nigrosin stain (to determine cell viability) and would like to be > able to somehow stain different segments of the sperm so that I can > identify and measure them using an ocular micrometer on a bright > field or phase contrast microscope set up. It is my understanding > that I cannot use fluorescent stains given that I have already used a > colormetric stain (the eosin-nigrosin). If anyone has suggestions for > ways to identify discrete regions of avian (passerine) sperm cells I > would be very grateful to hear from you. > > Many thanks in advance, > melissah > > ----- > melissah rowe > > PhD candidate > Department of Ecology & Evolution > University of Chicago > E. 57th Street, Chicago, IL, 60637 > ph: +1 773-702-3070 > fax: + 1 773-702-9740 > > email: melissah@uchicago.edu > > ------------------------------ > > Message: 6 > Date: Wed, 05 Sep 2007 14:39:04 -0500 > From: "Thomas Pier" > Subject: Re: [Histonet] IHC anti-cleaved caspase 3 > To: , > Message-ID: <46DEBF88020000DF0000A736@gwmail.medicine.wisc.edu> > Content-Type: text/plain; charset=US-ASCII > > Cell Signalling Technology has a good rabbit monoclonal for Cleaved > Caspase-3. > > Tom Pier > > >>> "Carl Hobbs" 09/05/07 2:29 PM >>> > Hi. > > Recent Cleaved caspase 3 posts have not mentioned the antibody details: I > would be grateful for the source of these Abs. > Carl > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 7 > Date: Wed, 5 Sep 2007 15:54:35 -0400 > From: Cheryl Cross > Subject: Re: [Histonet] IHC anti-cleaved caspase 3 > To: "Thomas Pier" > Cc: carl.hobbs@kcl.ac.uk, histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed > > I will second the Cell Signaling antibody - we have tried antibodies > from Promega (which gave tons of background staining)...then via this > site i was pointed to Cell Signaling's monoclonal which we tried; the > polyclonal actually has been working very well with minimal > background (we were getting lots of respiratory epithelium and > endothelium lighting up in control animals). I have been using it in > mice with this protocol: > > EDTA with PASCAL > primary antibody 60 minutes, dilution 1:125 > Rabbit envision > DAB plus > > Hope this helps! > > Cheryl Cross, DVM, Dipl. ACVP > Researcher > University Corporation for Atmospheric Research > College of Veterinary Medicine > University of Tennessee Department of Pathology > 2407 River Drive, Room A201 > Knoxville, TN 37996-4542 > (423) 967-2724 > fax: 865-974-5616 > ccross@ucar.edu > > > > > ------------------------------ > > Message: 8 > Date: Wed, 5 Sep 2007 15:59:45 -0400 > From: "Victoria Baker" > Subject: [Histonet] Fwd: Users of Lab Vision autostainer > To: "Histo Net list server" > Message-ID: > <4f016b690709051259rcdffccet70d7f97ae0457707@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > One more try, first one didn't go through it seems! Thanks > > ---------- Forwarded message ---------- > From: Victoria Baker > Date: Sep 5, 2007 9:02 AM > Subject: Users of Lab Vision autostainer > To: Histo Net list server > > Hi > > I'm a new user of the Lab Vision autostainer and I'm looking to see if > I can find users in Histo-land that have experience with it. The > facility only works with human tissue and all of the antibodies are > for dx purposes. > > My key questions are as follows: > How many antibodies is your lab running? > How many users do you allow? > How many people do you allow programming rights and at what level of > supervision are they? > > How many of these antibodies are from Lab Vision? > a) are they concentrates or pre-dilutes? > b) for HIER are you using their PT modules/procedures or > your own equipment (microwave, steamer, pressure cooker etc) and in > house designed protocols for retrieval? > c) for digestion do you only use their Pro-K or have you > designed your own in-house methods using other reagents for digestion? > d) do you put your controls on the same slide as the patient? > e) are your controls in-house or commercial? > f) do you have more than one stainer hooked up to one computer > system? > > What Version of software do you currently have on your system? > > Any feed back would be very helpful. > > Thanks in advance. > > Vikki Baker > Interim Histology Manager > Mission Hospital System > Asheville, NC > > ------------------------------ > > Message: 9 > Date: Wed, 05 Sep 2007 16:00:47 -0400 > From: Phil McArdle > Subject: Re: [Histonet] Microwaves VENDOR RESPONSE > To: Joe Nocito > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <46DF0AEF.7030203@ebsciences.com> > Content-Type: text/plain; charset=UTF-8; format=flowed > > Hi Joe: > > Obviously, a microwave vendor hates to hear microwave horror stories, so > again, no argument - even though I'm not privy to details of the fried > biopsies in question or what type/vintage of microwave, anyone who's > experienced a malfunction involving patient samples doesn't want a > repeat performance. And pathology is, must be, risk averse. > > That said, again, any mechanical or electronic equipment can fail, or > user error can contribute; just look at the "hang-up" problems with > older tissue processors that are now ancient history. It's up to > manufacturers to minimize the possibilities of failure, since patient > care is at stake. Improvement is therefore a continual, ongoing process. > For example, while for years EBS microwave processors incorporated > safety shutdown modes in the event of vent failure, probe failure (open > and closed) and many other component-related issues, about two years ago > we determined that the microwave did not have a comprehensive set of > safeguards to deal with user errors, for example, temperature overshoots > caused by too small a container for a given power setting, or failure to > place the temperature probe in solution. So we developed multiple safety > mechanisms to head off user errors of this sort. > > PMM > -- > Phil McArdle > Microwave Product Manager > > Energy Beam Sciences, Inc. > 29-B Kripes Rd. > East Granby, CT 06026 > > Tel: 800.992.9037 x 341 > Mobile: 860.597.6796 > Fax: 860.653.0422 > > pmcardle@ebsciences.com > www.ebsciences.com > > Joe Nocito wrote: > > are you sure it's the willies and not the johnnies? > > Since the magnetron or whatever it was that fried my tissue, I'd wait > > for the traditional processing. Call me a dinosaur, but I really don't > > like doing special stains in the microwave. The only thing I use a > > microwave for at my house is to defrost and reheat stuff (technical > > term). I'm sure there are people out there who can cook a 6 course > > gourmet meal. My best friend can process all types of tissue from > > biopsies to uterus. > > As a matter of fact, he was there grossing when something went wrong > > and told me that he's never seen tissue like that before. > > > > JTT > > ----- Original Message ----- From: "Phil McArdle" > > > > To: "Joe Nocito" > > Cc: > > Sent: Wednesday, September 05, 2007 11:16 AM > > Subject: Re: [Histonet] Microwaves VENDOR RESPONSE > > > > > >> Hi Joe: > >> > >> No argument there. I'm painfully aware of both a mindset of "a > >> microwave 'should' cost less than $100," and of a dearth of funding > >> for pathology in general (popular shows like CSI to the contrary). :-) > >> I'd still suggest that $1749 is a heck of a lot better (and a lot less > >> laughable) than the $18,000 or $30,000 that's widely quoted and > >> posted, and it's the exact reason we brought an under-$2000 lab > >> microwave to market in the first place. > >> > >> One could argue just as convincingly against all kinds of specialized > >> equipment or reagents on the basis of cost, not just microwaves. We > >> all know of everything from saliva to cheap rice steamers being used > >> in histo labs, and while they may actually be perfectly serviceable, > >> from the standpoint of repeatability or liability, this kind of thing > >> gives me the willies (and that's a technical term). My yardstick is > >> always "what would I be comfortable with if my kid's diagnosis hung in > >> the balance?" > >> > >> Healthy debate is good! > >> > >> Phil > >> -- > >> Phil McArdle > >> Microwave Product Manager > >> > >> Energy Beam Sciences, Inc. > >> 29-B Kripes Rd. > >> East Granby, CT 06026 > >> > >> Tel: 800.992.9037 x 341 > >> Mobile: 860.597.6796 > >> Fax: 860.653.0422 > >> > >> pmcardle@ebsciences.com > >> www.ebsciences.com > >> > >> > >> > >> > >> > >> > >> Joe Nocito wrote: > >>> ok, but with the budgets today, many people can't afford a $1749 > >>> microwave when they can buy one at Walmart, K-Mart, or somewhere else > >>> for $79. > >>> Not to make you angry or anything, but I'm wondering how long has > >>> it been since you worked in a lab? Histo's budget is the first one > >>> cut in the lab because we are not essential. > >>> I can't count how many times I fought and fought for my budgets. > >>> If I tried to justify a $1749 microwave for special stains, HIER > >>> or whatever, I would have been laughed out the manager's office. > >>> Just my 4 cents. > >>> > >>> JTT > >>> ----- Original Message ----- From: "Phil McArdle" > >>> > >>> To: "Kathleen Boozer" > >>> Cc: > >>> Sent: Wednesday, September 05, 2007 9:07 AM > >>> Subject: Re: [Histonet] Microwaves VENDOR RESPONSE > >>> > >>> > >>>> Again, a microwave vendor weighs in (so far I haven't received any > >>>> flames), so read at your own risk. :-) > >>>> > >>>> At the risk of sounding overly and overtly commercial, after reading > >>>> post after post of $30,000+ and $18,000 and similarly high figures > >>>> for lab microwaves, I really feel the need to set the record > >>>> straight. Depending on the usage requirements, we have laboratory > >>>> microwaves as low as $1749 for a "bare bones" model for simple > >>>> operations, to mid-priced units, to under $11,000 for a vacuum > >>>> equipped microwave processor capable of the +/- 0.5 degree C > >>>> temperature control necessary for tissue processing. > >>>> > >>>> (I can feel the heat already!) > >>>> > >>>> There are many compelling reasons to replace a kitchen microwave > >>>> with a lab model; feel free to download, read, and even share with > >>>> colleagues our Microwave Companion at > >>>> > >>>> http://www.ebsciences.com/pdf/EBS_MW_COMPANION.pdf > >>>> > >>>> Best regards, and see you at NSH, > >>>> > >>>> Phil McArdle > >>>> > >>>> -- > >>>> Phil McArdle > >>>> Microwave Product Manager > >>>> > >>>> Energy Beam Sciences, Inc. > >>>> 29-B Kripes Rd. > >>>> East Granby, CT 06026 > >>>> > >>>> Tel: 800.992.9037 x 341 > >>>> Mobile: 860.597.6796 > >>>> Fax: 860.653.0422 > >>>> > >>>> pmcardle@ebsciences.com > >>>> www.ebsciences.com > >>>> > >>>> Kathleen Boozer wrote: > >>>>> What is the best microwave for a small lab using it only for > >>>>> heating Bouin's and Silver Nitrate for special stains? I just > >>>>> can't believe I would have to spend $30,000+ or slow down and use a > >>>>> waterbath. > >>>>> > >>>>> > >>>>> _______________________________________________ > >>>>> Histonet mailing list > >>>>> Histonet@lists.utsouthwestern.edu > >>>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >>>> > >>>> > >>>> > >>>> > >>>> I skate to where the puck is going to be, not to where it's been. > >>>> - Wayne Gretsky > >>>> > >>>> You must be the change you want to see in the world. > >>>> - Mahatma Gandhi > >>>> > >>>> NOTE: This message, together with any attachments, is intended only > >>>> for the use of the individual or entity to which it is addressed and > >>>> may contain information that is legally privileged, confidential and > >>>> exempt from disclosure. If you are not the intended recipient, > >>>> however, there's not a lot I can do about it, and it was probably my > >>>> mistake anyway. So please do the right thing and make this e-mail go > >>>> away. Thank you. > >>>> > >>>> _______________________________________________ > >>>> Histonet mailing list > >>>> Histonet@lists.utsouthwestern.edu > >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >>> > >>> > >>> _______________________________________________ > >>> Histonet mailing list > >>> Histonet@lists.utsouthwestern.edu > >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > >> > >> -- > >> Phil McArdle > >> Microwave Product Manager > >> > >> Energy Beam Sciences, Inc. > >> 29-B Kripes Rd. > >> East Granby, CT 06026 > >> > >> Tel: 800.992.9037 x 341 > >> Mobile: 860.597.6796 > >> Fax: 860.653.0422 > >> > >> pmcardle@ebsciences.com > >> www.ebsciences.com > >> > >> I skate to where the puck is going to be, not to where it's been. > >> - Wayne Gretsky > >> > >> You must be the change you want to see in the world. > >> - Mahatma Gandhi > >> > >> NOTE: This message, together with any attachments, is intended only > >> for the use of the individual or entity to which it is addressed and > >> may contain information that is legally privileged, confidential and > >> exempt from disclosure. If you are not the intended recipient, > >> however, there's not a lot I can do about it, and it was probably my > >> mistake anyway. So please do the right thing and make this e-mail go > >> away. Thank you. > > > > I skate to where the puck is going to be, not to where it's been. > - Wayne Gretsky > > You must be the change you want to see in the world. > - Mahatma Gandhi > > NOTE: This message, together with any attachments, is intended only for > the use of the individual or entity to which it is addressed and may > contain information that is legally privileged, confidential and exempt > from disclosure. If you are not the intended recipient, however, there's > not a lot I can do about it, and it was probably my mistake anyway. So > please do the right thing and make this e-mail go away. Thank you. > > ------------------------------ > > Message: 10 > Date: Wed, 5 Sep 2007 13:01:21 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Xylene > To: themagoos@rushmore.com, histonet@lists.utsouthwestern.edu > Message-ID: <329330.36844.qm@web61219.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Tissues usually become brittle, which difficulties sectioning. > Ren? J. > > themagoos wrote: > Can somebody tell me if there are any effects on tissue if > there is prolonged processing time in xylene? > > Jason McGough HT(ASCP) > Clinical Laboratory of the Black Hills > Account Representative - Anatomic Pathology > 2805 5th Street > Rapid City, SD 57701 > 605-343-2267 > jmcgough@clinlab.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > --------------------------------- > Boardwalk for $500? In 2007? Ha! > Play Monopoly Here and Now (it's updated for today's economy) at Yahoo! > Games. > > ------------------------------ > > Message: 11 > Date: Wed, 5 Sep 2007 14:09:02 -0600 > From: "Liz Chlipala" > Subject: RE: [Histonet] IHC anti-cleaved caspase 3 > To: "Cheryl Cross" , "Thomas Pier" > > Cc: carl.hobbs@kcl.ac.uk, histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset="windows-1250" > > I like the cell signaling antibody also, I tried biocare's but did not > have > much success with it. The cell signaling antibody also works with pronase > digestion, as well as the EDTA pH9 HIER. We have even used it on bone > sections with the pronase digestion. Works in multiple species, I have > used it > on human, rat, mouse, guinea pig, porcine and canine. Our protocol is > similar > to Cheryl's. It’s a bit pricy as antibodies go, but I feel its > worth it. > Good lot to lot consistency. We have probably gone through about 5 > different > lots, with not changing the protocol at all. I have a written protocol if > anyone is interested. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Manager > Premier Laboratory, LLC > P.O. Box 18592 > Boulder, CO 80308 > phone (303) 735-5001 > fax (303) 735-3540 > liz@premierlab.com > www.premierlab.com > > Ship to Address: > > Premier Laboratory, LLC > University of Colorado at Boulder > MCDB, Room A3B40 > Boulder, CO 80309 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Cross Sent: > Wednesday, > September 05, 2007 2:01 PM To: Thomas Pier Cc: carl.hobbs@kcl.ac.uk; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] IHC anti-cleaved caspase 3 > > I will second the Cell Signaling antibody - we have tried antibodies from > Promega (which gave tons of background staining)...then via this site i > was > pointed to Cell Signaling's monoclonal which we tried; the polyclonal > actually > has been working very well with minimal background (we were getting lots > of > respiratory epithelium and endothelium lighting up in control animals). I > have > been using it in mice with this protocol: > > EDTA with PASCAL > primary antibody 60 minutes, dilution 1:125 Rabbit envision DAB plus > > Hope this helps! > > Cheryl Cross, DVM, Dipl. ACVP > Researcher > University Corporation for Atmospheric Research College of Veterinary > Medicine > University of Tennessee Department of Pathology 2407 River Drive, Room > A201 > Knoxville, TN 37996-4542 > (423) 967-2724 fax: 865-974-5616 ccross@ucar.edu > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.5.485 / Virus Database: 269.13.5/990 - Release Date: 9/4/2007 > 10:36 > PM > > No virus found in this outgoing message. > Checked by AVG Free Edition. > Version: 7.5.485 / Virus Database: 269.13.5/990 - Release Date: 9/4/2007 > 10:36 > PM > > ------------------------------ > > Message: 12 > Date: Wed, 05 Sep 2007 16:13:36 -0400 > From: Sarah Clatterbuck Soper > Subject: [Histonet] Freezing mouse testes for frozen sections > To: histonet@lists.utsouthwestern.edu > Message-ID: <46DF0DF0.2030306@ciwemb.edu> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Hi all, > > I've started attempting to section unfixed frozen mouse testes in order > to placate a specific antibody we have to use. I am new to frozen > sections and I'm having trouble with the testes cracking when I freeze > them. I've tried both freezing in isopentane cooled on liquid nitrogen > and an acetone/dry ice slurry. Either way the testes crack, usually one > big crack end to end. Doesn't seem to be as much of a problem with our > mutant testes, which are about 1/3 the size of wild-type. I wish I > could just trim the wild-type down to a smaller size, but obviously > that's not an option! > > Any recommendations? > > Thanks so much! > > Sarah > > ------------------------------ > > Message: 13 > Date: Wed, 5 Sep 2007 15:49:48 -0500 > From: "Mike Pence" > Subject: [Histonet] Olympus BX40 > To: > Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C701@IS-E2K3.grhs.net> > Content-Type: text/plain; charset="us-ascii" > > Need some help, > > I am looking for a 60x objective for an Olympus microscope BX40. > Would anyone know where I might get a used one or if they even make one > this size for this scope? > > Thanks, > Mike > > ------------------------------ > > Message: 14 > Date: Wed, 5 Sep 2007 16:58:12 -0400 > From: "Yu, Jian" > Subject: [Histonet] Look for a used stereoscope > To: > Message-ID: > <7E0A77BFEB9A1E47A63F978E7116821F07986D6D@1upmc-msx11.acct.upmchs.net> > Content-Type: text/plain; charset="us-ascii" > > Does anyone know a good place to get a used stereoscope? I plan to use > it to examine intestinal tumors in mice. > > Thanks a lot for your information. > > ******************************************************************* > Jian Yu, Ph.D. > University of Pittsburgh Cancer Institute > Hillman Cancer Center Research Pavilion > Office Suite 2.26h > 5117 Centre Avenue, Pittsburgh, PA 15213 > ******************************************************************* > > ------------------------------ > > Message: 15 > Date: Thu, 6 Sep 2007 09:44:45 +1000 > From: "Tony Henwood" > Subject: RE: [Histonet] RDO decal > To: "Rene J Buesa" , "RENEE FISHER" > , > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > There is also the issue of EDTA, which is common in many RDO formulations. > Checking one MSDS for EDTA reveals: > > Ecological Information > Environmental Fate: > When released into the soil, this material is expected to leach into > groundwater. When released into the soil, this material may biodegrade > to a > moderate extent. When released into the soil, this material is not > expected > to evaporate significantly. When released into water, this material is > not > expected to evaporate significantly. This material is not expected to > significantly bioaccumulate. When released into the air, this material is > expected to be readily degraded by photolysis. Environmental Toxicity: > This material is not expected to be toxic to aquatic life. The > LC50/96-hour > values for fish are over 100 mg/l. > > So neutralisation may not be required. > If anyone has info to the contrary please advise. > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) > Laboratory Manager & Senior Scientist > The Children's Hospital at Westmead, > Locked Bag 4001, Westmead, 2145, AUSTRALIA. > Tel: 612 9845 3306 > Fax: 612 9845 3318 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: > Thursday, 6 > September 2007 1:07 AM To: RENEE FISHER; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] RDO decal > > Ren?e: > I have not heard of neutralizing RDO, but it would make sense if you > want to > be "gentle on your sewer system" BUT prepare to a large emission of carbon > dioxide when attempting to neutralize it with baking soda. RDO + baking > soda > (or sodium bicarbonate) will produce water, salt with the acid in RDO > (probably sodium chloride or common salt), and carbon dioxide in > stoichiometrical amounts (1 CO2 per every 1 NaCl). Therefore that > neutralization has to take place in a fumes hood, and you will have to > decide > which is worst: delivering acid to the sewer system, or carbon dioxide > (the > Greenhouse gas per excellence) to the atmosphere. It will be "your call". > Ren? J. > > RENEE FISHER wrote: > Has anyone heard of neutralizing RDO with baking soda to P.H. 7.0. Our > Histo > lab had a hazardous waste assessment, and the consultant suggested we not > throw the RDO down the drain but that we either collect it for waste > removal > or neutralize it with baking soda to p.h. 7.0. I have not heard of doing > this > and do not know of any procedure for it, everyone, anyone's help will be > greatly appreciated. > > Thanks, > Renee' > > _______________________________________________________________________________________ > > This email may contain confidential protected health information and/or > proprietary information belonging to the sender that is legally privileged > under local, state, or federal law. This information is intended only for > the > use of the individual or individuals who have received this. The > authorized > recipient of this information is prohibited from disclosing this > information > to any other party unless required to do so by law. If you are not the > intended recipient, you are hereby notified that any disclosure, copying, > distribution, or action taken in reliance on the contents of this email > is > strictly prohibited. If you have received this email in error, please > notify > the sender immediately to arrange for the disposal of this information. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > --------------------------------- > Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: mail, > news, > photos & more. _______________________________________________ Histonet > mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************* > This email and any files transmitted with it are confidential and intended > solely for the use of the individual or entity to whom they are addressed. > If > you are not the intended recipient, please delete it and notify the > sender. > > Views expressed in this message and any attachments are those of the > individual sender, and are not necessarily the views of The Children's > Hospital at Westmead > > This note also confirms that this email message has been > virus scanned and although no computer viruses were detected, The > Childrens > Hospital at Westmead accepts no liability for any consequential damage > resulting from email containing computer viruses. ********************************************************************** > > ------------------------------ > > Message: 16 > Date: Thu, 6 Sep 2007 11:23:00 +0900 > From: ChoiUl Soo > Subject: [Histonet] PTH antibody reacted with dog tissue... > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="ks_c_5601-1987" > > Hi Histonetters, > > I am interested in anti PTH antibody reacted with dog tissue. > If anyone has successful experience with any PTH antibody with dog tissue, > please tell me one. > > I have searched through the internet, and results came back with abcam and > SantaCruz PTH antibodies predicted to react with them. But they are not > sure > on it, just predicted on the basis of sequence homology. > (abcam say 94% homology with human) I don't have good exprience with Santa > Cruz, and never used one by Abcam. Should I rely on it, or find another > one? > > Let me hear your experience. > > I would appreciate your advice or comment on this. > > Thank you~. > > Ul Soo Choi, DVM, PhDKRF priority zoonotic disease research institute, > College > of Veterinary Medicine, Seoul National University, Shilim9 dong, Gwanakgu, > Seoul, Korea 151-742Tel. 82-02-880-8688 Mobile. 82-016-9228-8634Fax. > 82-02-880- > 8662 > > _________________________________________________________________ > ???? ?????? ????, Windows Live Space! > http://www.spaces.live.com > > ------------------------------ > > Message: 17 > Date: Thu, 6 Sep 2007 01:57:04 -0400 > From: "Michelle McCoy" > Subject: [Histonet] Floater sources > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <25355ef80709052257i32794198gd5a64b94758cc1ea@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > I was reading some of the old archived messages on floaters, and being > somewhat new to the field of histotechnology was curious about how > floaters > can be attributed to a particular tech who performed work on the block. > For > example, couldn't other sources of contamination be from the automatic > stainer, processor carryover (cassette not completely closed/or if closed, > friable tissue through the slats eg if sponge not used on larger > specimen). > In some previous labs I've worked in- floaters were quickly attributed to > the cutter, embedder or grosser and might result in a "write up". If the > floater is seen in the block how can you differentiate if it came from the > grosser carryover/embedder carryover/processing carryover/unsigned > re-embedder/or even possibly even client carry over between patients or > different specimen types of the same patient. > And if it is not in the block --differentiating between the > cutter/automatic > stainer (I've seen specks of tissue debris in automatic stainers/ sections > falling off the slides and into the reagents etc). Obviously all should be > done to minimize the factors, but I'm just not clear how a single source > is > pinpointed and potentially blamed for the event (depending on the lab > policy). Thanks for any ideas on this. > > ------------------------------ > > Message: 18 > Date: Thu, 6 Sep 2007 08:07:38 +0100 > From: "Kemlo Rogerson" > Subject: RE: [Histonet] Xylene > To: , > Message-ID: > <86ADE4EB583CE64799A9924684A0FBBF0222EC6C@wahtntex2.waht.swest.nhs.uk> > Content-Type: text/plain; charset="us-ascii" > > Can somebody tell me if there are any effects on tissue if there is > prolonged processing time in xylene? > > Jason McGough HT(ASCP) > Clinical Laboratory of the Black Hills > Account Representative - Anatomic Pathology > 2805 5th Street > Rapid City, SD 57701 > 605-343-2267 > jmcgough@clinlab.com > > Classically it is said to harden, plus more lipids could be removed. > Personally I'm equivocal about that thought; if properly fixed prior to > processing I would have thought the hardening effects of xylene were > much less than that of the coagulant fixative ethanol. If you do have > hard tissue then there is a restorative fluid one can use which I think > has oil of cedarwood in it but I don't know the formula off hand. I know > it works cos when I was a pup I used it sometimes. > > Kemlo Rogerson > Pathology Manager > DD 01934 647057 or extension 3311 > Mob 07749 754194; Pager 07659 597107; > > Sunshine is delicious, rain is refreshing, wind braces us up, snow is > exhilarating; there is really no such thing as bad weather, only > different kinds of good weather. --John Ruskin > > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. > Please inform me that this message has gone astray before deleting it. > Thank you for your co-operation > > ------------------------------ > > Message: 19 > Date: Thu, 6 Sep 2007 08:17:36 +0100 > From: "Kemlo Rogerson" > Subject: RE: [Histonet] Floater sources > To: "Michelle McCoy" , > > Message-ID: > <86ADE4EB583CE64799A9924684A0FBBF0222EC6D@wahtntex2.waht.swest.nhs.uk> > Content-Type: text/plain; charset="us-ascii" > > I was reading some of the old archived messages on floaters, and being > somewhat new to the field of histotechnology was curious about how > floaters can be attributed to a particular tech who performed work on > the block. For example, couldn't other sources of contamination be from > the automatic stainer, processor carryover (cassette not completely > closed/or if closed, friable tissue through the slats eg if sponge not > used on larger specimen). In some previous labs I've worked in- floaters > were quickly attributed to the cutter, embedder or grosser and might > result in a "write up". If the floater is seen in the block how can you > differentiate if it came from the grosser carryover/embedder > carryover/processing carryover/unsigned re-embedder/or even possibly > even client carry over between patients or different specimen types of > the same patient. And if it is not in the block --differentiating > between the cutter/automatic stainer (I've seen specks of tissue debris > in automatic stainers/ sections falling off the slides and into the > reagents etc). Obviously all should be done to minimize the factors, but > I'm just not clear how a single source is pinpointed and potentially > blamed for the event (depending on the lab policy). Thanks for any ideas > on this. > > You are exactly correct, floaters can be attributed to a variety of > causes, processing machines that aren't regularly changed, transfer on > the cutting up forceps, on the waterbath and on the forceps of the > embedder. If the floater was from a block that was cut, embedded, cut up > before the section with the floater then the culprit is obvious. If the > section was cut by someone who didn't cut the block from which the > floater floated, then the culprit is the embedder, machine,or cutter up. > Realisticaly I would have thought that most floaters would be from the > embedder, cutter up, then sectioner as the latter has the greater > likelihood of seeing the error of his/ her ways. Waxy forceps of messy > forceps, in my experience are the usual culprit but in some instances, > necrotic tumours can shed cells in the processor and even the stainer. > > Floaters are usually obvious as they tend to be at a different level > than the tissue section and I'm afraid they are a fact of life. You can > reduce the incidence but sadly never eradicate them. > > Kemlo Rogerson > Pathology Manager > DD 01934 647057 or extension 3311 > Mob 07749 754194; Pager 07659 597107; > > Sunshine is delicious, rain is refreshing, wind braces us up, snow is > exhilarating; there is really no such thing as bad weather, only > different kinds of good weather. --John Ruskin > > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. > Please inform me that this message has gone astray before deleting it. > Thank you for your co-operation > > ------------------------------ > > Message: 20 > Date: Thu, 6 Sep 2007 04:18:04 -0400 > From: "Victoria Baker" > Subject: [Histonet] Fwd: Users of Lab Vision autostainer > To: histonet > Message-ID: > <4f016b690709060118t532d22c1if250116e0a6c9af1@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > ---------- Forwarded message ---------- > From: Victoria Baker > Date: Sep 5, 2007 9:02 AM > Subject: Users of Lab Vision autostainer > To: Histo Net list server > > Hi > > I'm a new user of the Lab Vision autostainer and I'm looking to see if > I can find users in Histo-land that have experience with it. The > facility only works with human tissue and all of the antibodies are > for dx purposes. > > My key questions are as follows: > How many antibodies is your lab running? > How many users do you allow? > How many people do you allow programming rights and at what level of > supervision are they? > > How many of these antibodies are from Lab Vision? > a) are they concentrates or pre-dilutes? > b) for HIER are you using their PT modules/procedures or > your own equipment (microwave, steamer, pressure cooker etc) and in > house designed protocols for retrieval? > c) for digestion do you only use their Pro-K or have you > designed your own in-house methods using other reagents for digestion? > d) do you put your controls on the same slide as the patient? > e) are your controls in-house or commercial? > f) do you have more than one stainer hooked up to one computer > system? > > What Version of software do you currently have on your system? > > Any feed back would be very helpful. > > Thanks in advance. > > Vikki Baker > Interim Histology Manager > Mission Hospital System > Asheville, NC > > ------------------------------ > > Message: 21 > Date: Thu, 6 Sep 2007 02:28:57 -0600 > From: "Joseph Kapler" > Subject: RE: [Histonet] Xylene > To: "Rene J Buesa" , , > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Tissues processed in Xylene (especially over long periods of time) the > tissue becomes very brittle. > > Hope that is the response you were looking for. > > Joseph "DarkWolfe" Kapler > I'm an outsider outside of everything > I'm an outsider outside of everything > I'm an outsider outside of everything > Everything you know. Everything you know > It disturbs me so > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J > Buesa > Sent: Wednesday, September 05, 2007 14:01 > To: themagoos@rushmore.com; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Xylene > > Tissues usually become brittle, which difficulties sectioning. > Ren? J. > > themagoos wrote: > Can somebody tell me if there are any effects on tissue if > there is prolonged processing time in xylene? > > Jason McGough HT(ASCP) > Clinical Laboratory of the Black Hills > Account Representative - Anatomic Pathology > 2805 5th Street > Rapid City, SD 57701 > 605-343-2267 > jmcgough@clinlab.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > --------------------------------- > Boardwalk for $500? In 2007? Ha! > Play Monopoly Here and Now (it's updated for today's economy) at Yahoo! > Games. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.5.485 / Virus Database: 269.13.5/988 - Release Date: 9/4/2007 > 09:14 > > No virus found in this outgoing message. > Checked by AVG Free Edition. > Version: 7.5.485 / Virus Database: 269.13.5/988 - Release Date: 9/4/2007 > 09:14 > > ------------------------------ > > Message: 22 > Date: Thu, 6 Sep 2007 09:59:13 +0100 > From: Malam Jacqueline > Subject: [Histonet] Unsubscribe > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain > > Please would you unsubscribe me as I am retiring tomorrow - thanks > > Jacqui malam > Lancaster > uk > > DISCLAIMER: This e-mail is confidential and privileged. If you are not the > intended recipient please accept our apologies; please do not disclose, > copy > or distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. Please > inform postmaster@rli.mbht.nhs.uk that this message has gone astray before > deleting it. Comments or opinions expressed in this email are those of > their respective contributors only. The views expressed do not represent > the > views of the Trust, its management or employees. University Hospitals of > Morecambe Bay NHS Trust is not responsible and disclaims any and all > liability for the content of comments written within.Thank you for your > co-operation. > > ------------------------------ > > Message: 23 > Date: Thu, 6 Sep 2007 06:31:58 -0500 > From: "Joe Nocito" > Subject: Re: [Histonet] Floater sources > To: "Michelle McCoy" , > > Message-ID: <004201c7f079$8f5aa560$0202a8c0@yourxhtr8hvc4p> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > Michelle, > first. welcome to world of histology. > Let's begin at the grossing table- all grossers are trained to wipe off > the > table after each case- but that doesn't mean floaters can't happen, but > the > incidence is low > third- embedders should have been taught to clean the embedding area after > each case and at the end of the day, but this is a good source for > contamination. You can see this is the block. > fourth- the cutters should wipe off their waterbaths after each block. > This > probably is the most likely source of contamination. I've inspected some > labs where one waterbath was just covered with previous ribbons, attached > to > the side and floating on the waterbath. > fifth- in my experience, it is highly unlikely that floaters came from the > stainer just by the shear movement of the slides and water. > > The bottom line is that everyone needs to be neat and clean and be > meticulous. Remember, cleanliness is next to Godliness. > I had a testicular seminoma where no matter what we did, floaters still > were > on cervical bxs and endometrial bxs. The medical and I decided that we > would handle cases like these separately. They were processed, embedded, > cut > and stained separately. Once the case was completed, we changed all the > solutions on the tissue processor and stainer. Who ever cut the blocks had > to dismantle their knife holder and microtome to clean it thoroughly. A > pain > in the butt, but was a necessary evil. > > I hope this helped a little. Good luck. > > Joe The Toe > ----- Original Message ----- > From: "Michelle McCoy" > To: > Sent: Thursday, September 06, 2007 12:57 AM > Subject: [Histonet] Floater sources > > >I was reading some of the old archived messages on floaters, and being > > somewhat new to the field of histotechnology was curious about how > > floaters > > can be attributed to a particular tech who performed work on the block. > > For > > example, couldn't other sources of contamination be from the automatic > > stainer, processor carryover (cassette not completely closed/or if > > closed, > > friable tissue through the slats eg if sponge not used on larger > > specimen). > > In some previous labs I've worked in- floaters were quickly attributed > > to > > the cutter, embedder or grosser and might result in a "write up". If the > > floater is seen in the block how can you differentiate if it came from > > the > > grosser carryover/embedder carryover/processing carryover/unsigned > > re-embedder/or even possibly even client carry over between patients or > > different specimen types of the same patient. > > And if it is not in the block --differentiating between the > > cutter/automatic > > stainer (I've seen specks of tissue debris in automatic stainers/ > > sections > > falling off the slides and into the reagents etc). Obviously all should > > be > > done to minimize the factors, but I'm just not clear how a single source > > is > > pinpointed and potentially blamed for the event (depending on the lab > > policy). Thanks for any ideas on this. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 46, Issue 6 > *************************************** ------- End of Original Message ------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tcl133 <@t> psu.edu Fri Sep 7 07:53:06 2007 From: tcl133 <@t> psu.edu (Dena Lang) Date: Fri Sep 7 07:53:27 2007 Subject: [Histonet] unsubscribe Message-ID: <001001c7f14e$0c41f220$212b7680@kinesresearch.psu.edu> Could you please unsubscribe me, Thank you, Dean Lang Dean H. Lang, PhD Pennsylvania State University From mpence <@t> grhs.net Fri Sep 7 08:40:31 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Fri Sep 7 08:40:46 2007 Subject: [Histonet] slide labels In-Reply-To: <000b01c7f0e4$f9becfb0$e5600744@pereirafarm> Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C707@IS-E2K3.grhs.net> I have used these labels for years and I have never seen one come off if it is applied directly to the slide and not on top of any other label. The slide has to be clean and dry when applied. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pereirafamily Sent: Thursday, September 06, 2007 7:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slide labels Hey all, Is anyone experienced with using slide labels that supposedly don't come off with any chemicals? Do you also write on the slide and then put the sticker on? Our facility is transferring to these new labels from Shamrock and a few of us have some concerns. Could you please e-mail Marilyn Weiss at Marilyn.a.weiss@kp.org. Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Fri Sep 7 09:04:52 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 7 09:05:33 2007 Subject: [Histonet] Dimedone PAS In-Reply-To: References: Message-ID: <46E15A84.7030606@umdnj.edu> Buffered formalin or Bouin's work well for glycogen demonstration but an alcoholic fixative (alcoholic Bouin or formalin-alcohol-acetic acid) will almost certainly, be better. Geoff Denise Piontek wrote: > Is anyone out there familiar with this staining method to isolate glycogen? How about alternate fixation methods associated with the stain? Advice and references are greatly appreciated, > > Denise Bland-Piontek, HTL(ACSP)CTBS(AATB) > NIBRI > _________________________________________________________________ > News, entertainment and everything you care about at Live.com. Get it now! > http://www.live.com/getstarted.aspx_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From hhawkins <@t> utmb.edu Fri Sep 7 09:10:47 2007 From: hhawkins <@t> utmb.edu (Hawkins, Hal K.) Date: Fri Sep 7 09:11:06 2007 Subject: [Histonet] Dimedone PAS In-Reply-To: <46E15A84.7030606@umdnj.edu> Message-ID: For preservation of glycogen, it's important to keep the specimen cold during fixation. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Geoff McAuliffe Sent: Friday, September 07, 2007 9:05 AM To: Denise Piontek Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Dimedone PAS Buffered formalin or Bouin's work well for glycogen demonstration but an alcoholic fixative (alcoholic Bouin or formalin-alcohol-acetic acid) will almost certainly, be better. Geoff Denise Piontek wrote: > Is anyone out there familiar with this staining method to isolate glycogen? How about alternate fixation methods associated with the stain? Advice and references are greatly appreciated, > > Denise Bland-Piontek, HTL(ACSP)CTBS(AATB) > NIBRI > _________________________________________________________________ > News, entertainment and everything you care about at Live.com. Get it now! > http://www.live.com/getstarted.aspx_____________________________________ __________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Fri Sep 7 09:16:52 2007 From: relia1 <@t> earthlink.net (Pam Barker) Date: Fri Sep 7 09:17:42 2007 Subject: [Histonet] RELIA Job Alert - Great opportunity for New Grad or Junior Histo Tech - Learn MOHS in Los Angeles Message-ID: Hi Histonetters! I hope everybody is getting ready for a fun weekend...TGIF!!! I have a great opportunity for a new graduate or junior tech with a large dermatopathology practice in Los Angeles. The position is a daytime position - M-F and full time. My client offers excellent compensation, benefits, free parking and the opportunity to learn MOHS. If you know of anyone who might be interested please have them contact me. I can be reached at relia1@earthlink.net or toll free at 866-607-3542. For a complete listing of my current openings nationwide for histotechnicians, histotechnologists and histology supervisors and managers please go to www.jobvertise.com and search keyword: RELIA Remember... it never hurts to look!! Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net From gu.lang <@t> gmx.at Fri Sep 7 09:48:07 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Sep 7 09:48:25 2007 Subject: AW: [Histonet] Acetone fixation for immunofluorescence protocols In-Reply-To: <1b2831cd0709061241k57f14da7n121a9b8f58912b04@mail.gmail.com> Message-ID: <004301c7f15e$1b0aa210$6412a8c0@dielangs.at> We usually let the frozen slides airdry for 30-120 min and then begin with IF without fixation. We do IF on human skin (IgG, IgM, IgA, C3c, Fbg). It seems to work, because there were no complaints. - but our pathologists haven't got any comparison. ;) Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Mauricio Avigdor Gesendet: Donnerstag, 06. September 2007 21:42 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Acetone fixation for immunofluorescence protocols Greetings: I am having a bit of difficulty with an immunofluorescence protocol (IF). I am using fresh tissue fixed for 10 minutes in cold acetone. Are there any fixatives that are well suited to IF protocols? Has anyone attempted to stain unfixed tissue? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Fri Sep 7 10:15:34 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 7 10:17:39 2007 Subject: [Histonet] FW: Emailing: junk6024.jpg Message-ID: <407F05A128805F4C879A33DBA32E618E01895037@TRFT-EX01.xRothGen.nhs.uk> Pink disease. Stain again, it will have disappeared. No known cause. (Histonet does not allow attachments, so picture did not get through.) Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Demarinis, Carolyn Sent: 07 September 2007 12:15 To: HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] FW: Emailing: junk6024.jpg We are getting pink blobs on our H&E and special stain slides with no nuclei. Can anyone tell me what is causing this? Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Fri Sep 7 12:18:08 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 7 12:18:37 2007 Subject: [Histonet] protocol for clearing tissue! In-Reply-To: References: Message-ID: The clearing agent with the highest refractive index is benzyl benzoate (1.57). It provides glass-like transparency. Methyl salicylate (1.54) is just as good, but the strong wintergreen smell can get a bit tiresome. Methyl benzoate (1.51) is also used for this purpose, but it has a really unpleasant odour so I don't recommend it. If the specimens do not have to be kept for a long time, xylene (R.I. 1.50) might be good enough. John Kiernan Anatomy, UWO London, Canada --- ----- Original Message ----- From: "Vest, Luther" Date: Thursday, September 6, 2007 13:51 Subject: [Histonet] protocol for clearing tissue! To: histonet@lists.utsouthwestern.edu > Dear histonet: > > One of the pathology residents in our department has asked me to post > this question. > > > > I am in search of a protocol for clearing tissue. This technique > is used > commonly in vascular research to visualize blood vessels. I want > to use > it to clear mouse lung tissue that contains blue-stained > (B-galactosidase activity) tumor nodules. Any suggestions? > > Thanks > > > > Luther Vest (HT ASCP) > > VA-MD Regional College of Veterinary Medicine > > Duckpond Dr. Phase III > > Blacksburg, Va 24061-0443 > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mickie25 <@t> netzero.net Fri Sep 7 12:34:13 2007 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Fri Sep 7 12:34:43 2007 Subject: [Histonet] Mohs Histology Training Seminar In-Reply-To: <287998.99715.qm@web52506.mail.re2.yahoo.com> References: <287998.99715.qm@web52506.mail.re2.yahoo.com> Message-ID: Dear Histonetters For any Mohs histo techs or histotechs interested in Mohs histology out there, I will be presenting a Mohs Advanced Training Seminar with Barbara Strippoli in New Jersey in early October. If any of you are interested in expanding your expertise in this growing field, we invite you to attend. I will be happy to forward more information to any replies. This is a two day seminar with hands-on practice using Leica cryostats. We still have some space available. Thank you for your time. Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net From GDawson <@t> dynacaremilwaukee.com Fri Sep 7 12:57:30 2007 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Fri Sep 7 12:57:48 2007 Subject: [Histonet] Gloves for Microtomy In-Reply-To: Message-ID: All, I have a rogue JCAHO inspector currently on-site that is demanding that all of the histotechs cut paraffin blocks with gloves on. She has made up many other rules of her own but most were so outlandish that they were easily discounted. What I am looking for is some kind of written policy out there that addresses the fact that it is not a requirement to wear gloves when handling/cutting paraffin blocks. We have tried to explain all of the reasons why it is not a requirement but she steadfastly refuses to listen to a single word from any peons she is inspecting. Since reasoning has no effect, I am looking for a manual of some sort that is published and available for purchase to provide written proof, although I'm not sure if it will make any difference to this particular individual. Any help would be GREATLY appreciated. Thanx In Advance, Glen Dawson IHC Manager Milwaukee, WI From tkngflght <@t> yahoo.com Fri Sep 7 13:09:53 2007 From: tkngflght <@t> yahoo.com (Cheryl R. Kerry) Date: Fri Sep 7 13:09:41 2007 Subject: [Histonet] Gloves for Microtomy In-Reply-To: Message-ID: <029f01c7f17a$4a8bf9a0$6701a8c0@CHERYLSLAPTOP> This brings to mind a story a histotech shared (maybe even on here) regarding her safety officer giving her grief about techs working bare-handed when cutting being a bio-hazardous situation. They argued back and forth and they were about to have to concede to the safety person's demands for gloves and the Histo Manager out of desperation--a person of many years' experience and with a sound, stable reputation in her lalb--picked up a faced block and licked it. The topic was never raised again. I know that doesn't help your situation but I hope it at least brought a smile :) Cheryl Kerry, HT(ASCP) Full Staff Inc. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Friday, September 07, 2007 12:58 PM Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gloves for Microtomy All, I have a rogue JCAHO inspector currently on-site that is demanding that all of the histotechs cut paraffin blocks with gloves on. She has made up many other rules of her own but most were so outlandish that they were easily discounted. What I am looking for is some kind of written policy out there that addresses the fact that it is not a requirement to wear gloves when handling/cutting paraffin blocks. We have tried to explain all of the reasons why it is not a requirement but she steadfastly refuses to listen to a single word from any peons she is inspecting. Since reasoning has no effect, I am looking for a manual of some sort that is published and available for purchase to provide written proof, although I'm not sure if it will make any difference to this particular individual. Any help would be GREATLY appreciated. Thanx In Advance, Glen Dawson IHC Manager Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tp2 <@t> medicine.wisc.edu Fri Sep 7 13:25:52 2007 From: tp2 <@t> medicine.wisc.edu (Thomas Pier) Date: Fri Sep 7 13:26:29 2007 Subject: [Histonet] Gloves for Microtomy Message-ID: <46E15161020000DF0000A8AD@gwmail.medicine.wisc.edu> I can't help with any sort of written protocol, but I can tell you that the only time I've known of a lab where you were required to wear gloves while cutting paraffin sections was when I worked in TSE (transmissable spongiform encepholopathies) lab. This was understandable given the nature of prions. Unless your dealing with suspected CJD case, I see no reason to take such measures. Tom Pier >>> "Dawson, Glen" 09/07/07 12:57 PM >>> All, I have a rogue JCAHO inspector currently on-site that is demanding that all of the histotechs cut paraffin blocks with gloves on. She has made up many other rules of her own but most were so outlandish that they were easily discounted. What I am looking for is some kind of written policy out there that addresses the fact that it is not a requirement to wear gloves when handling/cutting paraffin blocks. We have tried to explain all of the reasons why it is not a requirement but she steadfastly refuses to listen to a single word from any peons she is inspecting. Since reasoning has no effect, I am looking for a manual of some sort that is published and available for purchase to provide written proof, although I'm not sure if it will make any difference to this particular individual. Any help would be GREATLY appreciated. Thanx In Advance, Glen Dawson IHC Manager Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From slappycraw <@t> yahoo.com Fri Sep 7 13:27:51 2007 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Fri Sep 7 13:28:09 2007 Subject: [Histonet] Gloves for Microtomy In-Reply-To: <029f01c7f17a$4a8bf9a0$6701a8c0@CHERYLSLAPTOP> Message-ID: <776.33250.qm@web53608.mail.re2.yahoo.com> When safety is out of control we are all in danger. "Cheryl R. Kerry" wrote: This brings to mind a story a histotech shared (maybe even on here) regarding her safety officer giving her grief about techs working bare-handed when cutting being a bio-hazardous situation. They argued back and forth and they were about to have to concede to the safety person's demands for gloves and the Histo Manager out of desperation--a person of many years' experience and with a sound, stable reputation in her lalb--picked up a faced block and licked it. The topic was never raised again. I know that doesn't help your situation but I hope it at least brought a smile :) Cheryl Kerry, HT(ASCP) Full Staff Inc. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Friday, September 07, 2007 12:58 PM Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gloves for Microtomy All, I have a rogue JCAHO inspector currently on-site that is demanding that all of the histotechs cut paraffin blocks with gloves on. She has made up many other rules of her own but most were so outlandish that they were easily discounted. What I am looking for is some kind of written policy out there that addresses the fact that it is not a requirement to wear gloves when handling/cutting paraffin blocks. We have tried to explain all of the reasons why it is not a requirement but she steadfastly refuses to listen to a single word from any peons she is inspecting. Since reasoning has no effect, I am looking for a manual of some sort that is published and available for purchase to provide written proof, although I'm not sure if it will make any difference to this particular individual. Any help would be GREATLY appreciated. Thanx In Advance, Glen Dawson IHC Manager Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Moody friends. Drama queens. Your life? Nope! - their life, your story. Play Sims Stories at Yahoo! Games. From alaskagirl1950 <@t> yahoo.com Fri Sep 7 13:32:43 2007 From: alaskagirl1950 <@t> yahoo.com (Patricia Adams) Date: Fri Sep 7 13:32:55 2007 Subject: [Histonet] Gloves for Microtomy In-Reply-To: <776.33250.qm@web53608.mail.re2.yahoo.com> Message-ID: <966171.92306.qm@web52501.mail.re2.yahoo.com> I am sorry, what safety is out of control? The only time I am in danger is when I decide my fingers are too long. Patricia --- Larry Woody wrote: > When safety is out of control we are all in > danger. > > "Cheryl R. Kerry" wrote: > This brings to mind a story a histotech shared > (maybe even on here) > regarding her safety officer giving her grief > about techs working > bare-handed when cutting being a bio-hazardous > situation. > > They argued back and forth and they were about > to have to concede to the > safety person's demands for gloves and the > Histo Manager out of > desperation--a person of many years' experience > and with a sound, stable > reputation in her lalb--picked up a faced block > and licked it. > > The topic was never raised again. > > I know that doesn't help your situation but I > hope it at least brought a > smile :) > > Cheryl Kerry, HT(ASCP) > Full Staff Inc. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Dawson, Glen > Sent: Friday, September 07, 2007 12:58 PM > Cc: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Gloves for Microtomy > > All, > > I have a rogue JCAHO inspector currently > on-site that is demanding that all > of the histotechs cut paraffin blocks with > gloves on. She has made up many > other rules of her own but most were so > outlandish that they were easily > discounted. > > What I am looking for is some kind of written > policy out there that > addresses the fact that it is not a requirement > to wear gloves when > handling/cutting paraffin blocks. We have tried > to explain all of the > reasons why it is not a requirement but she > steadfastly refuses to listen to > a single word from any peons she is inspecting. > Since reasoning has no > effect, I am looking for a manual of some sort > that is published and > available for purchase to provide written > proof, although I'm not sure if it > will make any difference to this particular > individual. > > Any help would be GREATLY appreciated. > > Thanx In Advance, > > Glen Dawson > IHC Manager > Milwaukee, WI > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Moody friends. Drama queens. Your life? Nope! - > their life, your story. > Play Sims Stories at Yahoo! Games. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D ____________________________________________________________________________________ Moody friends. Drama queens. Your life? Nope! - their life, your story. Play Sims Stories at Yahoo! Games. http://sims.yahoo.com/ From doug <@t> ppspath.com Fri Sep 7 14:40:53 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Fri Sep 7 13:43:35 2007 Subject: {SPAM?} RE: [Histonet] Gloves for Microtomy In-Reply-To: <029f01c7f17a$4a8bf9a0$6701a8c0@CHERYLSLAPTOP> Message-ID: This whole thing just burns me. I was fuming over this same thing earlier this month. The latest edition of advance magazine has a gloved hand with someone cutting on the front cover. It also shows another picture of someone cutting with gloves. When these photos get into the wrong hands (administrators, safety officers, know-it-alls) then it causes nothing but problems. I would think that advance magazine would know that these photos are unrealistic. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl R. Kerry Sent: Friday, September 07, 2007 1:10 PM To: 'Dawson, Glen' Cc: histonet@lists.utsouthwestern.edu Subject: {SPAM?} RE: [Histonet] Gloves for Microtomy This brings to mind a story a histotech shared (maybe even on here) regarding her safety officer giving her grief about techs working bare-handed when cutting being a bio-hazardous situation. They argued back and forth and they were about to have to concede to the safety person's demands for gloves and the Histo Manager out of desperation--a person of many years' experience and with a sound, stable reputation in her lalb--picked up a faced block and licked it. The topic was never raised again. I know that doesn't help your situation but I hope it at least brought a smile :) Cheryl Kerry, HT(ASCP) Full Staff Inc. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Friday, September 07, 2007 12:58 PM Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gloves for Microtomy All, I have a rogue JCAHO inspector currently on-site that is demanding that all of the histotechs cut paraffin blocks with gloves on. She has made up many other rules of her own but most were so outlandish that they were easily discounted. What I am looking for is some kind of written policy out there that addresses the fact that it is not a requirement to wear gloves when handling/cutting paraffin blocks. We have tried to explain all of the reasons why it is not a requirement but she steadfastly refuses to listen to a single word from any peons she is inspecting. Since reasoning has no effect, I am looking for a manual of some sort that is published and available for purchase to provide written proof, although I'm not sure if it will make any difference to this particular individual. Any help would be GREATLY appreciated. Thanx In Advance, Glen Dawson IHC Manager Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> CDC.GOV Fri Sep 7 13:51:22 2007 From: jqb7 <@t> CDC.GOV (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Fri Sep 7 13:51:46 2007 Subject: {SPAM?} RE: [Histonet] Gloves for Microtomy In-Reply-To: References: <029f01c7f17a$4a8bf9a0$6701a8c0@CHERYLSLAPTOP> Message-ID: <34BB307EFC9A65429BBB49E330675F7202BDBFFF@LTA3VS003.ees.hhs.gov> I thought the exact same thing when I saw my copy! Gloves while cutting? Jeanine Bartlett Infectious Disease Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Friday, September 07, 2007 3:41 PM To: 'Cheryl R. Kerry'; 'Dawson, Glen' Cc: histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} RE: [Histonet] Gloves for Microtomy This whole thing just burns me. I was fuming over this same thing earlier this month. The latest edition of advance magazine has a gloved hand with someone cutting on the front cover. It also shows another picture of someone cutting with gloves. When these photos get into the wrong hands (administrators, safety officers, know-it-alls) then it causes nothing but problems. I would think that advance magazine would know that these photos are unrealistic. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl R. Kerry Sent: Friday, September 07, 2007 1:10 PM To: 'Dawson, Glen' Cc: histonet@lists.utsouthwestern.edu Subject: {SPAM?} RE: [Histonet] Gloves for Microtomy This brings to mind a story a histotech shared (maybe even on here) regarding her safety officer giving her grief about techs working bare-handed when cutting being a bio-hazardous situation. They argued back and forth and they were about to have to concede to the safety person's demands for gloves and the Histo Manager out of desperation--a person of many years' experience and with a sound, stable reputation in her lalb--picked up a faced block and licked it. The topic was never raised again. I know that doesn't help your situation but I hope it at least brought a smile :) Cheryl Kerry, HT(ASCP) Full Staff Inc. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Friday, September 07, 2007 12:58 PM Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gloves for Microtomy All, I have a rogue JCAHO inspector currently on-site that is demanding that all of the histotechs cut paraffin blocks with gloves on. She has made up many other rules of her own but most were so outlandish that they were easily discounted. What I am looking for is some kind of written policy out there that addresses the fact that it is not a requirement to wear gloves when handling/cutting paraffin blocks. We have tried to explain all of the reasons why it is not a requirement but she steadfastly refuses to listen to a single word from any peons she is inspecting. Since reasoning has no effect, I am looking for a manual of some sort that is published and available for purchase to provide written proof, although I'm not sure if it will make any difference to this particular individual. Any help would be GREATLY appreciated. Thanx In Advance, Glen Dawson IHC Manager Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mohs76009 <@t> yahoo.com Fri Sep 7 13:57:11 2007 From: mohs76009 <@t> yahoo.com (Matt Bancroft) Date: Fri Sep 7 13:57:23 2007 Subject: {SPAM?} RE: [Histonet] Gloves for Microtomy Message-ID: <465919.38472.qm@web63409.mail.re1.yahoo.com> I do wear gloves while cutting to prevent squams. It took a while to get use to. Douglas D Deltour wrote: This whole thing just burns me. I was fuming over this same thing earlier this month. The latest edition of advance magazine has a gloved hand with someone cutting on the front cover. It also shows another picture of someone cutting with gloves. When these photos get into the wrong hands (administrators, safety officers, know-it-alls) then it causes nothing but problems. I would think that advance magazine would know that these photos are unrealistic. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl R. Kerry Sent: Friday, September 07, 2007 1:10 PM To: 'Dawson, Glen' Cc: histonet@lists.utsouthwestern.edu Subject: {SPAM?} RE: [Histonet] Gloves for Microtomy This brings to mind a story a histotech shared (maybe even on here) regarding her safety officer giving her grief about techs working bare-handed when cutting being a bio-hazardous situation. They argued back and forth and they were about to have to concede to the safety person's demands for gloves and the Histo Manager out of desperation--a person of many years' experience and with a sound, stable reputation in her lalb--picked up a faced block and licked it. The topic was never raised again. I know that doesn't help your situation but I hope it at least brought a smile :) Cheryl Kerry, HT(ASCP) Full Staff Inc. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Friday, September 07, 2007 12:58 PM Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gloves for Microtomy All, I have a rogue JCAHO inspector currently on-site that is demanding that all of the histotechs cut paraffin blocks with gloves on. She has made up many other rules of her own but most were so outlandish that they were easily discounted. What I am looking for is some kind of written policy out there that addresses the fact that it is not a requirement to wear gloves when handling/cutting paraffin blocks. We have tried to explain all of the reasons why it is not a requirement but she steadfastly refuses to listen to a single word from any peons she is inspecting. Since reasoning has no effect, I am looking for a manual of some sort that is published and available for purchase to provide written proof, although I'm not sure if it will make any difference to this particular individual. Any help would be GREATLY appreciated. Thanx In Advance, Glen Dawson IHC Manager Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. From derek.papalegis <@t> tufts.edu Fri Sep 7 13:57:37 2007 From: derek.papalegis <@t> tufts.edu (Derek Papalegis) Date: Fri Sep 7 13:57:50 2007 Subject: [Histonet] Gloves for Microtomy In-Reply-To: <200709071844.l87Ii7Sr024319@mail-proofpoint-3a.usg.tufts.edu> References: <200709071844.l87Ii7Sr024319@mail-proofpoint-3a.usg.tufts.edu> Message-ID: <46E19F21.1060404@tufts.edu> Although it is not a biohazard risk to cut without gloves on, why would you want to? When you think about it, it is pretty gross. Although it is fixed, you are dealing with tissues such as cysts, prostate "chips", colon biopsies and other nasty tissues. Maybe it a generational thing but it seems like the newer histotechs would never think of cutting without gloves on. I know that some people claim they cant cut with gloves on and that is fine but if why not use gloves if you can. It's not a bad idea and it also prevents your hands from getting caked in paraffin. Just my 2 cents. -Derek Douglas D Deltour wrote: > This whole thing just burns me. I was fuming over this same thing earlier > this month. The latest edition of advance magazine has a gloved hand with > someone cutting on the front cover. It also shows another picture of someone > cutting with gloves. When these photos get into the wrong hands > (administrators, safety officers, know-it-alls) then it causes nothing but > problems. I would think that advance magazine would know that these photos > are unrealistic. > > Douglas D. Deltour HT(ASCP) > Histology Manager > Professional Pathology Services, PC > One Science Court > Suite 200 > Columbia, SC 29203 > Office (803)252-1913 > Fax (803)254-3262 > Doug@ppspath.com > > From dellav <@t> musc.edu Fri Sep 7 13:59:50 2007 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Fri Sep 7 14:00:15 2007 Subject: [Histonet] Gloves for Microtomy In-Reply-To: References: Message-ID: <7F6B678A32B0564196138E6B3101996AAEF693@EVS1.clinlan.local> Glen, I feel your pain. I will be very surprised if the documentation you've asked for actually exists. Often such requirements start out as NCCLS (I can never seem to remember this organization's current name) standards or guidelines and end up in accreditation checklists. That said, I've never seen statements in writing regarding the sectioning of paraffin blocks with the exception of the prion disorders and even that now is no longer a concern following the recommended formic acid pretreatment of tissues from prion suspected cases. I'm guessing your inspector is a physician. Have you request him/her to provide you with documentation that clearly states that gloves are required for the sectioning of chemically fixed/processed tissues? I assume she has taken the glove required for bloodborne pathogen protection and extended it to processed tissue. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Friday, September 07, 2007 1:58 PM Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gloves for Microtomy All, I have a rogue JCAHO inspector currently on-site that is demanding that all of the histotechs cut paraffin blocks with gloves on. She has made up many other rules of her own but most were so outlandish that they were easily discounted. What I am looking for is some kind of written policy out there that addresses the fact that it is not a requirement to wear gloves when handling/cutting paraffin blocks. We have tried to explain all of the reasons why it is not a requirement but she steadfastly refuses to listen to a single word from any peons she is inspecting. Since reasoning has no effect, I am looking for a manual of some sort that is published and available for purchase to provide written proof, although I'm not sure if it will make any difference to this particular individual. Any help would be GREATLY appreciated. Thanx In Advance, Glen Dawson IHC Manager Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Fri Sep 7 14:03:06 2007 From: tkngflght <@t> yahoo.com (Cheryl R. Kerry) Date: Fri Sep 7 14:02:54 2007 Subject: [Histonet] Histonet history question In-Reply-To: <46E19F21.1060404@tufts.edu> Message-ID: <02b801c7f181$b9e8b7a0$6701a8c0@CHERYLSLAPTOP> Hi Everyone-- Can someone tell me when the Histonet was started and approximately how many people there are registered and in how many countries? Just curious as to how big our 'voice' might be.... Cheryl Kerry, HT(ASCP) Full Staff Inc. From bill501 <@t> mindspring.com Fri Sep 7 14:14:20 2007 From: bill501 <@t> mindspring.com (Bill) Date: Fri Sep 7 14:14:35 2007 Subject: [Histonet] Gloves for Microtomy In-Reply-To: References: Message-ID: I have formally placed lab inspectors in the same category as lawyers, regulators and politicians. m|m At 12:57 PM -0500 9/7/07, Dawson, Glen wrote: >I have a rogue JCAHO inspector currently on-site that is demanding >that all of the histotechs cut paraffin blocks with gloves on. She >has made up many other rules of her own but most were so outlandish >that they were easily discounted. -- _____________________________ Bill Blank http://kernunnos.com (Celtic studies and numismatics) OBOD's Message board: http://www.druidry.org/board/dhp/ From GDawson <@t> dynacaremilwaukee.com Fri Sep 7 14:14:23 2007 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Fri Sep 7 14:14:38 2007 Subject: [Histonet] Gloves for Microtomy In-Reply-To: <7F6B678A32B0564196138E6B3101996AAEF693@EVS1.clinlan.local> Message-ID: Actually, the inspector is a nurse with on a major power trip. I have no words to describe the hostility, the attitude, etc... I know that there is probably nothing in writing that would address a regulation that doesn't exist but I'm grasping at straws here. You know you have a problem when your inspector starts off a directive with "In my opinion...". This is the end game when someone who knows nothing about a histolab is assigned to inspect it. I think Vinnie's suggestion is awesome & I'll try that but, if anyone does find something in writing addressing this non-existant regulation, give me a yell. Thanx, Glen D. -----Original Message----- From: Della Speranza, Vinnie [mailto:dellav@musc.edu] Sent: Friday, September 07, 2007 2:00 PM To: Dawson, Glen Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Gloves for Microtomy Glen, I feel your pain. I will be very surprised if the documentation you've asked for actually exists. Often such requirements start out as NCCLS (I can never seem to remember this organization's current name) standards or guidelines and end up in accreditation checklists. That said, I've never seen statements in writing regarding the sectioning of paraffin blocks with the exception of the prion disorders and even that now is no longer a concern following the recommended formic acid pretreatment of tissues from prion suspected cases. I'm guessing your inspector is a physician. Have you request him/her to provide you with documentation that clearly states that gloves are required for the sectioning of chemically fixed/processed tissues? I assume she has taken the glove required for bloodborne pathogen protection and extended it to processed tissue. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Friday, September 07, 2007 1:58 PM Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gloves for Microtomy All, I have a rogue JCAHO inspector currently on-site that is demanding that all of the histotechs cut paraffin blocks with gloves on. She has made up many other rules of her own but most were so outlandish that they were easily discounted. What I am looking for is some kind of written policy out there that addresses the fact that it is not a requirement to wear gloves when handling/cutting paraffin blocks. We have tried to explain all of the reasons why it is not a requirement but she steadfastly refuses to listen to a single word from any peons she is inspecting. Since reasoning has no effect, I am looking for a manual of some sort that is published and available for purchase to provide written proof, although I'm not sure if it will make any difference to this particular individual. Any help would be GREATLY appreciated. Thanx In Advance, Glen Dawson IHC Manager Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ander093 <@t> tc.umn.edu Fri Sep 7 14:20:17 2007 From: ander093 <@t> tc.umn.edu (LuAnn Anderson) Date: Fri Sep 7 14:20:43 2007 Subject: [Histonet] fixation containers In-Reply-To: <7F6B678A32B0564196138E6B3101996AAEF693@EVS1.clinlan.local> References: <7F6B678A32B0564196138E6B3101996AAEF693@EVS1.clinlan.local> Message-ID: Hi all, Happy Friday!! I am wondering if anyone can provide me with a vender who makes containers large enough to fix whole, suspended brains (approx. 4 gallon or ~15 liter with cover). We always ordered them from Rubbermaid but they have now been discontinued. What are other Neuro people out there using? I have found some but they are pretty pricey!! Thanks for your help. LuAnn Anderson HT(ASCP) Neuropathology Laboratory University of Minnesota From Dolores_Fischer <@t> baxter.com Fri Sep 7 14:36:31 2007 From: Dolores_Fischer <@t> baxter.com (Dolores_Fischer@baxter.com) Date: Fri Sep 7 14:38:13 2007 Subject: [Histonet] Gloves for Microtomy In-Reply-To: Message-ID: I'm surprised that they don't require metal mesh gloves as well to prevent knife blade cuts. Maybe double gloving for added protection............................. D The information transmitted is intended only for the person(s)or entity to which it is addressed and may contain confidential and/or legally privileged material. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive privilege or confidentiality. Any review, retransmission, dissemination or other use of , or taking of any action in reliance upon, this information by entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. For Translation: http://www.baxter.com/email_disclaimer From rjbuesa <@t> yahoo.com Fri Sep 7 14:39:03 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Sep 7 14:39:15 2007 Subject: [Histonet] Gloves for Microtomy In-Reply-To: Message-ID: <368311.17261.qm@web61216.mail.yahoo.com> Glen: You have taken the wrong approach: you are trying to get documentation to prove that you are innocent (right), but the "burden of proof" is with the inspector. S/he was to prove that you are at fault, s/he is the one that has to show you the documentation requiring that ALL blocks have to be sectioned wearing gloves. The next thing tha you have to do is PREPARE your OWN procedure and incorporate it to your SOP (after approval by the Lab. Director), requiring, for example, to wear gloves when dealing with KNOWN prion cases, and not requiering wearing gloves with other types of tissues. Finally, I don't know how the information has gotten out, but the year was 1998, and I was the one who licked the block! Ren? J. "Dawson, Glen" wrote: All, I have a rogue JCAHO inspector currently on-site that is demanding that all of the histotechs cut paraffin blocks with gloves on. She has made up many other rules of her own but most were so outlandish that they were easily discounted. What I am looking for is some kind of written policy out there that addresses the fact that it is not a requirement to wear gloves when handling/cutting paraffin blocks. We have tried to explain all of the reasons why it is not a requirement but she steadfastly refuses to listen to a single word from any peons she is inspecting. Since reasoning has no effect, I am looking for a manual of some sort that is published and available for purchase to provide written proof, although I'm not sure if it will make any difference to this particular individual. Any help would be GREATLY appreciated. Thanx In Advance, Glen Dawson IHC Manager Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Boardwalk for $500? In 2007? Ha! Play Monopoly Here and Now (it's updated for today's economy) at Yahoo! Games. From jnocito <@t> satx.rr.com Fri Sep 7 14:43:06 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Sep 7 14:43:25 2007 Subject: [Histonet] Gloves for Microtomy References: <029f01c7f17a$4a8bf9a0$6701a8c0@CHERYLSLAPTOP> Message-ID: <003c01c7f187$5085bf50$0202a8c0@yourxhtr8hvc4p> gee, I hope the block wasn't a cervical biopsy with HPV ----- Original Message ----- From: "Cheryl R. Kerry" To: "'Dawson, Glen'" Cc: Sent: Friday, September 07, 2007 1:09 PM Subject: RE: [Histonet] Gloves for Microtomy > This brings to mind a story a histotech shared (maybe even on here) > regarding her safety officer giving her grief about techs working > bare-handed when cutting being a bio-hazardous situation. > > They argued back and forth and they were about to have to concede to the > safety person's demands for gloves and the Histo Manager out of > desperation--a person of many years' experience and with a sound, stable > reputation in her lalb--picked up a faced block and licked it. > > The topic was never raised again. > > I know that doesn't help your situation but I hope it at least brought a > smile :) > > Cheryl Kerry, HT(ASCP) > Full Staff Inc. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, > Glen > Sent: Friday, September 07, 2007 12:58 PM > Cc: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Gloves for Microtomy > > All, > > I have a rogue JCAHO inspector currently on-site that is demanding that > all > of the histotechs cut paraffin blocks with gloves on. She has made up > many > other rules of her own but most were so outlandish that they were easily > discounted. > > What I am looking for is some kind of written policy out there that > addresses the fact that it is not a requirement to wear gloves when > handling/cutting paraffin blocks. We have tried to explain all of the > reasons why it is not a requirement but she steadfastly refuses to listen > to > a single word from any peons she is inspecting. Since reasoning has no > effect, I am looking for a manual of some sort that is published and > available for purchase to provide written proof, although I'm not sure if > it > will make any difference to this particular individual. > > Any help would be GREATLY appreciated. > > Thanx In Advance, > > Glen Dawson > IHC Manager > Milwaukee, WI > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Fri Sep 7 14:47:50 2007 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Fri Sep 7 14:48:03 2007 Subject: [Histonet] Gloves for Microtomy In-Reply-To: Message-ID: Don't give them any ideas!!! Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dolores_Fischer@baxter.com Sent: Friday, September 07, 2007 2:37 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Gloves for Microtomy I'm surprised that they don't require metal mesh gloves as well to prevent knife blade cuts. Maybe double gloving for added protection............................. D The information transmitted is intended only for the person(s)or entity to which it is addressed and may contain confidential and/or legally privileged material. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive privilege or confidentiality. Any review, retransmission, dissemination or other use of , or taking of any action in reliance upon, this information by entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. For Translation: http://www.baxter.com/email_disclaimer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Fri Sep 7 14:49:50 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Sep 7 14:50:08 2007 Subject: [Histonet] Gloves for Microtomy References: Message-ID: <005a01c7f188$42deea60$0202a8c0@yourxhtr8hvc4p> see, this is where you have someone not familiar with the department inspecting. They are taught to wear gloves all the time. Hello, they should because they are dealing with live people and not processed pieces. I can say this because I was a nursing student back in the early 90s. It was bat in our heads to wear gloves . As far as the inspector, I'm sorry Glenn, but stay in the field long enough, you do come across a wacked out inspector. Remember my story about the CAP inspector with his own thermometer? Well I think she should put her opinion where the CAP inspector put his thermometer. No, silly, not there. Back in his pocket. Just think that it's the weekend. Did someone yell Scotch? JTT ----- Original Message ----- From: "Dawson, Glen" Cc: Sent: Friday, September 07, 2007 2:14 PM Subject: RE: [Histonet] Gloves for Microtomy Actually, the inspector is a nurse with on a major power trip. I have no words to describe the hostility, the attitude, etc... I know that there is probably nothing in writing that would address a regulation that doesn't exist but I'm grasping at straws here. You know you have a problem when your inspector starts off a directive with "In my opinion...". This is the end game when someone who knows nothing about a histolab is assigned to inspect it. I think Vinnie's suggestion is awesome & I'll try that but, if anyone does find something in writing addressing this non-existant regulation, give me a yell. Thanx, Glen D. -----Original Message----- From: Della Speranza, Vinnie [mailto:dellav@musc.edu] Sent: Friday, September 07, 2007 2:00 PM To: Dawson, Glen Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Gloves for Microtomy Glen, I feel your pain. I will be very surprised if the documentation you've asked for actually exists. Often such requirements start out as NCCLS (I can never seem to remember this organization's current name) standards or guidelines and end up in accreditation checklists. That said, I've never seen statements in writing regarding the sectioning of paraffin blocks with the exception of the prion disorders and even that now is no longer a concern following the recommended formic acid pretreatment of tissues from prion suspected cases. I'm guessing your inspector is a physician. Have you request him/her to provide you with documentation that clearly states that gloves are required for the sectioning of chemically fixed/processed tissues? I assume she has taken the glove required for bloodborne pathogen protection and extended it to processed tissue. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Friday, September 07, 2007 1:58 PM Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gloves for Microtomy All, I have a rogue JCAHO inspector currently on-site that is demanding that all of the histotechs cut paraffin blocks with gloves on. She has made up many other rules of her own but most were so outlandish that they were easily discounted. What I am looking for is some kind of written policy out there that addresses the fact that it is not a requirement to wear gloves when handling/cutting paraffin blocks. We have tried to explain all of the reasons why it is not a requirement but she steadfastly refuses to listen to a single word from any peons she is inspecting. Since reasoning has no effect, I am looking for a manual of some sort that is published and available for purchase to provide written proof, although I'm not sure if it will make any difference to this particular individual. Any help would be GREATLY appreciated. Thanx In Advance, Glen Dawson IHC Manager Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Fri Sep 7 14:52:13 2007 From: tkngflght <@t> yahoo.com (Cheryl R. Kerry) Date: Fri Sep 7 14:52:04 2007 Subject: [Histonet] Gloves for Microtomy In-Reply-To: <368311.17261.qm@web61216.mail.yahoo.com> Message-ID: <02c501c7f188$9714fd90$6701a8c0@CHERYLSLAPTOP> It must've been you because for the longest time I thought you were a 'she'!! So sorry!! Cheryl -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, September 07, 2007 2:39 PM To: Dawson, Glen Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Gloves for Microtomy Glen: You have taken the wrong approach: you are trying to get documentation to prove that you are innocent (right), but the "burden of proof" is with the inspector. S/he was to prove that you are at fault, s/he is the one that has to show you the documentation requiring that ALL blocks have to be sectioned wearing gloves. The next thing tha you have to do is PREPARE your OWN procedure and incorporate it to your SOP (after approval by the Lab. Director), requiring, for example, to wear gloves when dealing with KNOWN prion cases, and not requiering wearing gloves with other types of tissues. Finally, I don't know how the information has gotten out, but the year was 1998, and I was the one who licked the block! Ren? J. "Dawson, Glen" wrote: All, I have a rogue JCAHO inspector currently on-site that is demanding that all of the histotechs cut paraffin blocks with gloves on. She has made up many other rules of her own but most were so outlandish that they were easily discounted. What I am looking for is some kind of written policy out there that addresses the fact that it is not a requirement to wear gloves when handling/cutting paraffin blocks. We have tried to explain all of the reasons why it is not a requirement but she steadfastly refuses to listen to a single word from any peons she is inspecting. Since reasoning has no effect, I am looking for a manual of some sort that is published and available for purchase to provide written proof, although I'm not sure if it will make any difference to this particular individual. Any help would be GREATLY appreciated. Thanx In Advance, Glen Dawson IHC Manager Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Boardwalk for $500? In 2007? Ha! Play Monopoly Here and Now (it's updated for today's economy) at Yahoo! Games. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AFoshey <@t> chw.org Fri Sep 7 14:40:14 2007 From: AFoshey <@t> chw.org (Foshey, Annette) Date: Fri Sep 7 14:55:26 2007 Subject: [Histonet] Users of the Leica ST5020 and CV5030 workstation Message-ID: <9E6D52F532809247BDA1783680E92C560B7028AB@CHWEXC.chwi.chswi.org> Histonetters, Please give me your feedback on the pro's and con's of the instrumentation. Your responses will be very valuable. Thanks, Annette Foshey, HT (ASCP) Team Leader in Histology Children's Hospital of Wisconsin 414-266-6580 Fax 414-266-2779 afoshey@chw.org ************************** Children's Hospital and Health System recognizes that unencrypted e-mail is insecure and does not guarantee confidentiality. The confidentiality of replies to this message cannot be guaranteed unless the replies are encrypted. From carl.hobbs <@t> kcl.ac.uk Fri Sep 7 15:18:54 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Fri Sep 7 15:19:12 2007 Subject: [Histonet] protocol for clearing tissue! Message-ID: <004401c7f18c$505b1390$4101a8c0@carlba65530bda> But, but.... is the resultant pigment stable in those media? After all, one would have to dehydrate beforehand? Curious Carl From turkekul <@t> gmail.com Fri Sep 7 16:14:41 2007 From: turkekul <@t> gmail.com (mesruh turkekul) Date: Fri Sep 7 16:14:55 2007 Subject: [Histonet] protocol for clearing tissues Message-ID: Hi I would strongly suggest to use BA/BB mixture for clearing. Here is the protocol for embryos but can be applied to lung as well I have tried. Methyl salicilate may not be good with 2-photon confocal and may not be suitbale for some pairs of fluorophores and is nasty with bad smell :) Clearing of tissues for microscopy with benzyl benzoate/benzyl alcohol (BA/BB) Benzyl benzoate (phenyl methyl benzoate) (refractive index 1.5685 to 1.570; mixable with alcohol, chloroform, ether and xylene). Benzyl alcohol BA/BB solution contains one volume of BA and two volumes of BB (BA: BB = 1:2) *Benzyl benzoate is intolerant of water! Keep it dessicated!!! *Use glass lab ware; BA/BB dissolves plastic!!! *Keep all reagents in the dark!!! Tissue preparation Dehydrate stained embryos (DAB or fluorescence staining) through ethanol series (70%, 95% and 100% ethanol). Transfer samples into 1:1 mixture of 100% ethanol:BA/BB until the embryos sink (for about 15 min, if sample is mounted on a slide/coverslip). Transfer samples in BA/BB and incubate until the sample becomes transparent. Perform imaging in BA/BB. Reverse the solvent series and store samples in PBS at 40C degrees in the dark. *BA/BB could gradually dissolve color products of alkaline phosphatase and B-galactosidase reaction and also fluorophores). Do not keep them long it BA/BB. Cheers, Mesru From Jason.Wiese <@t> va.gov Fri Sep 7 16:15:52 2007 From: Jason.Wiese <@t> va.gov (Wiese, Jason VHAROS) Date: Fri Sep 7 16:16:05 2007 Subject: [Histonet] Gloves for Microtomy In-Reply-To: <46E19F21.1060404@tufts.edu> References: <200709071844.l87Ii7Sr024319@mail-proofpoint-3a.usg.tufts.edu> <46E19F21.1060404@tufts.edu> Message-ID: <70EEF3D43B3C164C94037D811B2BE193E12CC8@VHAV20MSGA3.v20.med.va.gov> At the risk of being flamed... No disrespect, but you are in the wrong line of work my friend. If you think paraffin embedded tissue is "nasty", how do you feel about a cystic structure exploding up under your face mask on autopsy, or a necrotic bowel, or a gangrene amputation, or... well... a billion other things that make embedded tissue look like frosted flakes. This is just my 2 cents too, but the last of my worries is touching a "nasty" tissue block when cutting. Do you wear gloves when putting blocks in the storage containers too... or when disposing of old blocks? What is nasty to me is having my hand in a Nitrile glove for 4-5 hours! JW -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Derek Papalegis Sent: Friday, September 07, 2007 11:58 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Gloves for Microtomy Although it is not a biohazard risk to cut without gloves on, why would you want to? When you think about it, it is pretty gross. Although it is fixed, you are dealing with tissues such as cysts, prostate "chips", colon biopsies and other nasty tissues. Maybe it a generational thing but it seems like the newer histotechs would never think of cutting without gloves on. I know that some people claim they cant cut with gloves on and that is fine but if why not use gloves if you can. It's not a bad idea and it also prevents your hands from getting caked in paraffin. Just my 2 cents. -Derek Douglas D Deltour wrote: > This whole thing just burns me. I was fuming over this same thing earlier > this month. The latest edition of advance magazine has a gloved hand with > someone cutting on the front cover. It also shows another picture of someone > cutting with gloves. When these photos get into the wrong hands > (administrators, safety officers, know-it-alls) then it causes nothing but > problems. I would think that advance magazine would know that these photos > are unrealistic. > > Douglas D. Deltour HT(ASCP) > Histology Manager > Professional Pathology Services, PC > One Science Court > Suite 200 > Columbia, SC 29203 > Office (803)252-1913 > Fax (803)254-3262 > Doug@ppspath.com > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Fri Sep 7 16:55:41 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Sep 7 16:55:57 2007 Subject: [Histonet] fixation containers In-Reply-To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E273CDB@LSRIEXCH1.lsmaster.lifespan.org> This place sells polyethylene pails with lids, in 1, 2, 3.5, 5, and 6 gallon sizes. Cheap. www.ctvalleybio.com It's a pretty common item though, and I would think you could find it locally. Have you tried Home Depot or Lowe's? Or perhaps a paint store? From cwscouten <@t> myneurolab.com Fri Sep 7 17:23:25 2007 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Fri Sep 7 17:22:45 2007 Subject: [Histonet] damage to dura References: <295C5D4C-0D6F-40E1-B0A6-12AD7BF74325@bidmc.harvard.edu> Message-ID: <5784D843593D874C93E9BADCB87342AB03DA1788@tpiserver03.Coretech-holdings.com> Hi caroline. Not enough attention is paid to the "dimpling", dipping into cortex, that almost always occurs with free hand drilling. You seem to have a clear cut test of that. As I understand what you are saying, the behavior is different between untouched animals, and those with only a hole drilled through skull and resulting dimpling. You need to mount the drill on the stereotaxic instrument, and go down a precisely measured distance. We offer a manual drill for this, and our flexshaft drill can be mounted on the stereotaxic with the large probe holder. This is what I would choose: Manual Stereotaxic Drill Product: #463018 Cordially, Charles W.? Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 x 342 FAX? 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Caroline Bass Sent: Thursday, August 30, 2007 11:52 PM To: Histonet Subject: [Histonet] damage to dura Hey guys, i have a question I thought may be good for the group. I am doing stereotax surgeries on rats, basically making lesions in the prefrontal cortex. There are two potential sham controls, one where a vehicle is injected the other where the holes are drilled but nothing is injected. So here's the problem. In the no injection sham (i.e. only holes are drilled) the animals display an alteration of the behavior we're interested in. The tissue looks good, and there is no sign of infiltration of immune cells in the PFC by cresyl violet. However, the dura does have some damage where the drill pierced the skull. We use a standard dremmel. Are there any suggestions for what could be going on? Is there an immune response we should specifically look for, or is there something else about the dura that could be affecting our system? Does anyone know of how dura damage can alter nearby brain areas? Any and all advice is appreciated. Caroline _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Fri Sep 7 17:37:40 2007 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Fri Sep 7 17:37:53 2007 Subject: FW: [Histonet] fixation containers Message-ID: <57BE698966D5C54EAE8612E8941D768301268F05@EXCHANGE3.huntingtonhospital.com> -----Original Message----- From: Laurie Colbert Sent: Friday, September 07, 2007 3:35 PM To: 'LuAnn Anderson' Subject: RE: [Histonet] fixation containers LuAnn, I get 86 oz containers (large enough for a brain) from Fisher Scientific. Their item # is 02-544-209A. They are $34.57/cs and come 25 in a case. Laurie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of LuAnn Anderson Sent: Friday, September 07, 2007 12:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fixation containers Hi all, Happy Friday!! I am wondering if anyone can provide me with a vender who makes containers large enough to fix whole, suspended brains (approx. 4 gallon or ~15 liter with cover). We always ordered them from Rubbermaid but they have now been discontinued. What are other Neuro people out there using? I have found some but they are pretty pricey!! Thanks for your help. LuAnn Anderson HT(ASCP) Neuropathology Laboratory University of Minnesota _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Fri Sep 7 19:08:38 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Sep 7 19:09:07 2007 Subject: [Histonet] Gloves for Microtomy References: <200709071844.l87Ii7Sr024319@mail-proofpoint-3a.usg.tufts.edu> <46E19F21.1060404@tufts.edu> <70EEF3D43B3C164C94037D811B2BE193E12CC8@VHAV20MSGA3.v20.med.va.gov> Message-ID: <003601c7f1ac$6f83b540$0202a8c0@yourxhtr8hvc4p> Ok, story time. When I was in my PA training, I had to cut all the legs. One (sweet, yeah right) pathologist wanted me to strip the blood vessels all the way down to the ankle. I told her (my mistake) it didn't matter because if the blood vessels were occluded at the surgical margin, the doctors will most like have to go higher when the surgical incision doesn't heal. Well as you can guess, I lost that argument ( that was a no brainer). So, I started clamping off the blood vessels at the surgical margin. One time as I was stripping the blood vessels. I ran my scalpel across the vessels and got a mouthful of blood. Yep, tomato soup. Luckily, the patient had no infectious diseases (the hospital ran tests) and I told that pathologist, in front of the medical director if she wants to, she can gross legs, but I'm not stripping legs any more. It's okay, I don't work there any more. JTT ----- Original Message ----- From: "Wiese, Jason VHAROS" To: "Derek Papalegis" ; Sent: Friday, September 07, 2007 4:15 PM Subject: RE: [Histonet] Gloves for Microtomy At the risk of being flamed... No disrespect, but you are in the wrong line of work my friend. If you think paraffin embedded tissue is "nasty", how do you feel about a cystic structure exploding up under your face mask on autopsy, or a necrotic bowel, or a gangrene amputation, or... well... a billion other things that make embedded tissue look like frosted flakes. This is just my 2 cents too, but the last of my worries is touching a "nasty" tissue block when cutting. Do you wear gloves when putting blocks in the storage containers too... or when disposing of old blocks? What is nasty to me is having my hand in a Nitrile glove for 4-5 hours! JW -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Derek Papalegis Sent: Friday, September 07, 2007 11:58 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Gloves for Microtomy Although it is not a biohazard risk to cut without gloves on, why would you want to? When you think about it, it is pretty gross. Although it is fixed, you are dealing with tissues such as cysts, prostate "chips", colon biopsies and other nasty tissues. Maybe it a generational thing but it seems like the newer histotechs would never think of cutting without gloves on. I know that some people claim they cant cut with gloves on and that is fine but if why not use gloves if you can. It's not a bad idea and it also prevents your hands from getting caked in paraffin. Just my 2 cents. -Derek Douglas D Deltour wrote: > This whole thing just burns me. I was fuming over this same thing earlier > this month. The latest edition of advance magazine has a gloved hand with > someone cutting on the front cover. It also shows another picture of someone > cutting with gloves. When these photos get into the wrong hands > (administrators, safety officers, know-it-alls) then it causes nothing but > problems. I would think that advance magazine would know that these photos > are unrealistic. > > Douglas D. Deltour HT(ASCP) > Histology Manager > Professional Pathology Services, PC > One Science Court > Suite 200 > Columbia, SC 29203 > Office (803)252-1913 > Fax (803)254-3262 > Doug@ppspath.com > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jason.Wiese <@t> va.gov Fri Sep 7 19:34:25 2007 From: Jason.Wiese <@t> va.gov (Wiese, Jason VHAROS) Date: Fri Sep 7 19:34:40 2007 Subject: [Histonet] Gloves for Microtomy In-Reply-To: <003601c7f1ac$6f83b540$0202a8c0@yourxhtr8hvc4p> References: <200709071844.l87Ii7Sr024319@mail-proofpoint-3a.usg.tufts.edu> <46E19F21.1060404@tufts.edu> <70EEF3D43B3C164C94037D811B2BE193E12CC8@VHAV20MSGA3.v20.med.va.gov> <003601c7f1ac$6f83b540$0202a8c0@yourxhtr8hvc4p> Message-ID: <70EEF3D43B3C164C94037D811B2BE193E12CCB@VHAV20MSGA3.v20.med.va.gov> Nice! I got one for you... When I was doing my PA training I had a sweet(yeah right) pathologist make me run a necrotic bowel. I didn't see the point of running the whole thing, as it was rotten to the core, and putrid. The bowel twist was obvious, and the section was obviously cut off from it's blood supply. All obvious... obvious... obvious... She was just trying to make me tuff I guess, so she stood inches away from me so I could feel her breath on the back of my neck... She looked over my shoulder, and I think it was because she knew I hated it. She flipped up her face shield to gripe about something else right as the bowel slipped from my fingers. It hit the edge of the table and flicked like a straw full of your favorite drink... except there was no drink in it. Instead of me taking the bullet, I side stepped as it was falling, and she took a face full of... well, about the nastiest stuff you have even seen or smelled in your life. She shut right up and walked out of the room. She never looked over my shoulder again. Chalk one for the PA/HT who does all the work and gets little of the glory or money. :) Have a great weekend fellas! Jason -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: Friday, September 07, 2007 5:09 PM To: Wiese, Jason VHAROS; Derek Papalegis; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Gloves for Microtomy Ok, story time. When I was in my PA training, I had to cut all the legs. One (sweet, yeah right) pathologist wanted me to strip the blood vessels all the way down to the ankle. I told her (my mistake) it didn't matter because if the blood vessels were occluded at the surgical margin, the doctors will most like have to go higher when the surgical incision doesn't heal. Well as you can guess, I lost that argument ( that was a no brainer). So, I started clamping off the blood vessels at the surgical margin. One time as I was stripping the blood vessels. I ran my scalpel across the vessels and got a mouthful of blood. Yep, tomato soup. Luckily, the patient had no infectious diseases (the hospital ran tests) and I told that pathologist, in front of the medical director if she wants to, she can gross legs, but I'm not stripping legs any more. It's okay, I don't work there any more. JTT ----- Original Message ----- From: "Wiese, Jason VHAROS" To: "Derek Papalegis" ; Sent: Friday, September 07, 2007 4:15 PM Subject: RE: [Histonet] Gloves for Microtomy At the risk of being flamed... No disrespect, but you are in the wrong line of work my friend. If you think paraffin embedded tissue is "nasty", how do you feel about a cystic structure exploding up under your face mask on autopsy, or a necrotic bowel, or a gangrene amputation, or... well... a billion other things that make embedded tissue look like frosted flakes. This is just my 2 cents too, but the last of my worries is touching a "nasty" tissue block when cutting. Do you wear gloves when putting blocks in the storage containers too... or when disposing of old blocks? What is nasty to me is having my hand in a Nitrile glove for 4-5 hours! JW -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Derek Papalegis Sent: Friday, September 07, 2007 11:58 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Gloves for Microtomy Although it is not a biohazard risk to cut without gloves on, why would you want to? When you think about it, it is pretty gross. Although it is fixed, you are dealing with tissues such as cysts, prostate "chips", colon biopsies and other nasty tissues. Maybe it a generational thing but it seems like the newer histotechs would never think of cutting without gloves on. I know that some people claim they cant cut with gloves on and that is fine but if why not use gloves if you can. It's not a bad idea and it also prevents your hands from getting caked in paraffin. Just my 2 cents. -Derek Douglas D Deltour wrote: > This whole thing just burns me. I was fuming over this same thing earlier > this month. The latest edition of advance magazine has a gloved hand with > someone cutting on the front cover. It also shows another picture of someone > cutting with gloves. When these photos get into the wrong hands > (administrators, safety officers, know-it-alls) then it causes nothing but > problems. I would think that advance magazine would know that these photos > are unrealistic. > > Douglas D. Deltour HT(ASCP) > Histology Manager > Professional Pathology Services, PC > One Science Court > Suite 200 > Columbia, SC 29203 > Office (803)252-1913 > Fax (803)254-3262 > Doug@ppspath.com > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Sat Sep 8 04:08:25 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Sep 8 04:08:42 2007 Subject: [Histonet] re: gloves for microtomy In-Reply-To: <465919.38472.qm@web63409.mail.re1.yahoo.com> Message-ID: <001601c7f1f7$d0c4c100$6412a8c0@dielangs.at> Perhaps a strange question: Do you touch the tissue-slide while cutting? Is this due to the technique with rotary-microtoms? In our lab, we cut with sliding microtoms and take the slide with brushes. So no contact with my skin. Also in the waterbath I avoid wet fingers. I think there is no need for using gloves here. Gudrun Lang From jnocito <@t> satx.rr.com Sat Sep 8 06:26:32 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Sat Sep 8 06:26:49 2007 Subject: [Histonet] Gloves for Microtomy References: <200709071844.l87Ii7Sr024319@mail-proofpoint-3a.usg.tufts.edu> <46E19F21.1060404@tufts.edu> <70EEF3D43B3C164C94037D811B2BE193E12CC8@VHAV20MSGA3.v20.med.va.gov> <003601c7f1ac$6f83b540$0202a8c0@yourxhtr8hvc4p> <70EEF3D43B3C164C94037D811B2BE193E12CCB@VHAV20MSGA3.v20.med.va.gov> Message-ID: <004401c7f20b$1c3277a0$0202a8c0@yourxhtr8hvc4p> what a bunch of crap! Why do people have to be that way? Now you got me started. I consider myself somewhat intelligent (I know, others don't). I had a pathology resident one time just kept telling me how he was a doctor and I was "just" a tech. I was active duty Air Force at the time and supervising the Air Force's largest histo lab. He was an officer, I was enlisted. One day I just got so tired of "Me Doctor, You POS" I got into his face and told him that he had opportunities that I didn't have and it doesn't make me less intelligent than him. That's the last time I heard he "Me Doctor" speech. What does this have to do with gloves for microtomy? I have no idea. That's why I can't be a teacher or instructor, I go off on tangents like this. JTT ----- Original Message ----- From: "Wiese, Jason VHAROS" To: "Joe Nocito" ; "Derek Papalegis" ; Sent: Friday, September 07, 2007 7:34 PM Subject: RE: [Histonet] Gloves for Microtomy Nice! I got one for you... When I was doing my PA training I had a sweet(yeah right) pathologist make me run a necrotic bowel. I didn't see the point of running the whole thing, as it was rotten to the core, and putrid. The bowel twist was obvious, and the section was obviously cut off from it's blood supply. All obvious... obvious... obvious... She was just trying to make me tuff I guess, so she stood inches away from me so I could feel her breath on the back of my neck... She looked over my shoulder, and I think it was because she knew I hated it. She flipped up her face shield to gripe about something else right as the bowel slipped from my fingers. It hit the edge of the table and flicked like a straw full of your favorite drink... except there was no drink in it. Instead of me taking the bullet, I side stepped as it was falling, and she took a face full of... well, about the nastiest stuff you have even seen or smelled in your life. She shut right up and walked out of the room. She never looked over my shoulder again. Chalk one for the PA/HT who does all the work and gets little of the glory or money. :) Have a great weekend fellas! Jason -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: Friday, September 07, 2007 5:09 PM To: Wiese, Jason VHAROS; Derek Papalegis; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Gloves for Microtomy Ok, story time. When I was in my PA training, I had to cut all the legs. One (sweet, yeah right) pathologist wanted me to strip the blood vessels all the way down to the ankle. I told her (my mistake) it didn't matter because if the blood vessels were occluded at the surgical margin, the doctors will most like have to go higher when the surgical incision doesn't heal. Well as you can guess, I lost that argument ( that was a no brainer). So, I started clamping off the blood vessels at the surgical margin. One time as I was stripping the blood vessels. I ran my scalpel across the vessels and got a mouthful of blood. Yep, tomato soup. Luckily, the patient had no infectious diseases (the hospital ran tests) and I told that pathologist, in front of the medical director if she wants to, she can gross legs, but I'm not stripping legs any more. It's okay, I don't work there any more. JTT ----- Original Message ----- From: "Wiese, Jason VHAROS" To: "Derek Papalegis" ; Sent: Friday, September 07, 2007 4:15 PM Subject: RE: [Histonet] Gloves for Microtomy At the risk of being flamed... No disrespect, but you are in the wrong line of work my friend. If you think paraffin embedded tissue is "nasty", how do you feel about a cystic structure exploding up under your face mask on autopsy, or a necrotic bowel, or a gangrene amputation, or... well... a billion other things that make embedded tissue look like frosted flakes. This is just my 2 cents too, but the last of my worries is touching a "nasty" tissue block when cutting. Do you wear gloves when putting blocks in the storage containers too... or when disposing of old blocks? What is nasty to me is having my hand in a Nitrile glove for 4-5 hours! JW -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Derek Papalegis Sent: Friday, September 07, 2007 11:58 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Gloves for Microtomy Although it is not a biohazard risk to cut without gloves on, why would you want to? When you think about it, it is pretty gross. Although it is fixed, you are dealing with tissues such as cysts, prostate "chips", colon biopsies and other nasty tissues. Maybe it a generational thing but it seems like the newer histotechs would never think of cutting without gloves on. I know that some people claim they cant cut with gloves on and that is fine but if why not use gloves if you can. It's not a bad idea and it also prevents your hands from getting caked in paraffin. Just my 2 cents. -Derek Douglas D Deltour wrote: > This whole thing just burns me. I was fuming over this same thing earlier > this month. The latest edition of advance magazine has a gloved hand with > someone cutting on the front cover. It also shows another picture of someone > cutting with gloves. When these photos get into the wrong hands > (administrators, safety officers, know-it-alls) then it causes nothing but > problems. I would think that advance magazine would know that these photos > are unrealistic. > > Douglas D. Deltour HT(ASCP) > Histology Manager > Professional Pathology Services, PC > One Science Court > Suite 200 > Columbia, SC 29203 > Office (803)252-1913 > Fax (803)254-3262 > Doug@ppspath.com > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie <@t> conxis.com Sat Sep 8 09:30:13 2007 From: laurie <@t> conxis.com (Laurie Popp) Date: Sat Sep 8 09:30:40 2007 Subject: [Histonet] Re: Gloves Message-ID: <46E2B1F5.30006@conxis.com> Hi All, I just graduated from a Nccls program through Mayo/UND and we were not taught to cut wearing gloves, in fact there is only one tech in the lab that consistently wears gloves while cutting and she came to us from the enzyme histochemistry lab so she couldn't get used to not wearing gloves. As far as the mesh gloves, we have those also and are supposed to wear them whenever changing/handling blades per our safety officer because right after I started there was a rash of people cutting themselves. Ironically enough they are cut resistant but not cut proof because someone actually did cut themselves while wearing them. Only time I personally wear them is if I am cutting a known/ suspected prion case or if I have managed to gain a paper cut and am trying to keep my hands clean otherwise I find the static from the gloves to be annoying. Laurie Popp Mayo Clinic Rochester From clfields12 <@t> alltel.net Sat Sep 8 10:10:09 2007 From: clfields12 <@t> alltel.net (clfields12@alltel.net) Date: Sat Sep 8 10:10:21 2007 Subject: [Histonet] Job Openings Message-ID: <20070908151009.GMFT29331.ispmxmta06-srv.windstream.net@webmail-relay.alltel.net> Pathology Consultants is a fast growing Histology Lab located in Greenville,South Carolina. (associated with the Greenville Hospital System) Our newly built histology lab (opening in Oct.)is seeking full-time, experienced Histologists and PA Support Techs. We are building a strong team in this new facility. The Histo position is a daytime position, M-F with one Saturday every 4 to 5 weeks 1/2 day. Great benefits, free parking... Please call 1-864-295-8215 (ask for Carole)for more information and an appointment. Carole Fields, HT(ASCP) Histology Manager 8 Memorial Ct. Greenville, SC 29604 email resume to cfields@ghs.org From Jason.Burrill <@t> crl.com Sat Sep 8 19:35:21 2007 From: Jason.Burrill <@t> crl.com (Burrill, Jason) Date: Sat Sep 8 19:35:47 2007 Subject: [Histonet] RE: Gloves References: <67tift$87a3n@mail01.us.crl.com> Message-ID: <1AD4E907E9B6F648AEF1B3A20A9B0E1E0BDE67@shr-exch2.na01.crl.com> I just have to weigh in on behalf of Derek Papalegis. Although there are no major safety risks of handling paraffin embedded sections, prion diseases that are still transmittable after formalin fixation and processing being the exception, I think it is unfair to say that he, Derek, is in the wrong line of work. I can say this because I was Derek's first supervisor and trained him from scratch and I can say that he has done very well for himself in the past several years since entering the profession (he is already the supervisor of a lab with a little over 3 years experience). Please don't make assumptions about people posting their opinions or responding to a question, it is unfair. For the record I also agree with Derek that having your fingers caked in paraffin is not ideal and is a little gross and I have seen my fair share of "disgusting" samples working with both veterinary and human samples. In addition I can also say that all technologists in my lab wear gloves while sectioning and I don't think it is a problem as long as they are educated about the reasons they are wearing PPE and are trained appropriately. I invite Joe to flame me all he wants for my opinion since I started out in his Air Force lab a short 11 years ago and still have great respect for him and his experience. Jason Burrill Manager, Histology Charles River Laboratories 251 Ballardvale St Wilmington, MA 01887 Phone: 978-658-6000 ext. 1652 Fax: 978-988-8793 jason.burrill@crl.com From dcojita <@t> tampabay.rr.com Sat Sep 8 20:12:13 2007 From: dcojita <@t> tampabay.rr.com (dcojita@tampabay.rr.com) Date: Sat Sep 8 20:15:33 2007 Subject: [Histonet] Acetone fixation for immunofluorescence protocols In-Reply-To: <004301c7f15e$1b0aa210$6412a8c0@dielangs.at> Message-ID: What difficulty are you having with the IF? We airdry in a dessicator, place in cold acetone for 10 minutes and then airdry again (for a few minutes), then stain. Ours turns out perfect every time (all skins) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Friday, September 07, 2007 10:48 AM To: 'Mauricio Avigdor' Cc: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] Acetone fixation for immunofluorescence protocols We usually let the frozen slides airdry for 30-120 min and then begin with IF without fixation. We do IF on human skin (IgG, IgM, IgA, C3c, Fbg). It seems to work, because there were no complaints. - but our pathologists haven't got any comparison. ;) Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Mauricio Avigdor Gesendet: Donnerstag, 06. September 2007 21:42 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Acetone fixation for immunofluorescence protocols Greetings: I am having a bit of difficulty with an immunofluorescence protocol (IF). I am using fresh tissue fixed for 10 minutes in cold acetone. Are there any fixatives that are well suited to IF protocols? Has anyone attempted to stain unfixed tissue? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Sat Sep 8 20:20:41 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Sat Sep 8 20:20:58 2007 Subject: [Histonet] RE: Gloves References: <67tift$87a3n@mail01.us.crl.com> <1AD4E907E9B6F648AEF1B3A20A9B0E1E0BDE67@shr-exch2.na01.crl.com> Message-ID: <001401c7f27f$a3ca6ff0$0202a8c0@yourxhtr8hvc4p> Jason, flame you? Hell, I'm gonna nuke you. Nah, I have to agree with you to a point. Those of us who never or rarely are exposed (no pun intended) to prions & such become lax in routine labs. I'm not even going down he road that everything should be handled as infectious. I agree, I mean no one should be putting their bare hands into any solution in he lab. I've been in this job for 30 years and have went from smoking while coverslipping to wearing gloves, gowns, and face shields. I don't have any problems, any problems or or tics, tics. Ya know, come to think of it, all my kids have 7 toes. I just thought that they would be better in math than me. Later JTT ----- Original Message ----- From: "Burrill, Jason" To: Sent: Saturday, September 08, 2007 7:35 PM Subject: [Histonet] RE: Gloves I just have to weigh in on behalf of Derek Papalegis. Although there are no major safety risks of handling paraffin embedded sections, prion diseases that are still transmittable after formalin fixation and processing being the exception, I think it is unfair to say that he, Derek, is in the wrong line of work. I can say this because I was Derek's first supervisor and trained him from scratch and I can say that he has done very well for himself in the past several years since entering the profession (he is already the supervisor of a lab with a little over 3 years experience). Please don't make assumptions about people posting their opinions or responding to a question, it is unfair. For the record I also agree with Derek that having your fingers caked in paraffin is not ideal and is a little gross and I have seen my fair share of "disgusting" samples working with both veterinary and human samples. In addition I can also say that all technologists in my lab wear gloves while sectioning and I don't think it is a problem as long as they are educated about the reasons they are wearing PPE and are trained appropriately. I invite Joe to flame me all he wants for my opinion since I started out in his Air Force lab a short 11 years ago and still have great respect for him and his experience. Jason Burrill Manager, Histology Charles River Laboratories 251 Ballardvale St Wilmington, MA 01887 Phone: 978-658-6000 ext. 1652 Fax: 978-988-8793 jason.burrill@crl.com -------------------------------------------------------------------------------- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From agustinvictor <@t> gmail.com Sat Sep 8 22:35:03 2007 From: agustinvictor <@t> gmail.com (Agustin Chertcoff) Date: Sat Sep 8 22:35:55 2007 Subject: [Histonet] SAHNET Message-ID: <00fc01c7f292$6a601fe0$590215ac@Pentium4> Hi Histonetters! The SAHNET is a listserver (in Spanish Language) for the Histology profession that is managed as a service to the field of Histology by Ht Agustin Victor Chertcoff & Ht Norma Pozzo. SAHNET current has more than 245 members in the world specially Argentine,Peru,Mexico,Costa Rica,Uruguay,Chile,Colombia,Spain. go to http://www.elistas.net/grupo/sahnet visit to www.ht.org.ar Argentine Society of Histotechnology SAH Thanks to all! Agustin From talulahgosh <@t> gmail.com Sat Sep 8 23:19:42 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Sat Sep 8 23:19:53 2007 Subject: [Histonet] protocol for clearing tissues In-Reply-To: References: Message-ID: BABB clears our whole mount IHC very well, but we've noticed it gets everywhere and dissolves everything we use to view embryos, so we've moved to glass. It's also hard to wash away since it's insoluble in water and alcohol--at least that's our experience. Emily -- Remember our war hysteria, when we called sauerkraut 'Liberty cabbage' and somebody actually proposed calling German measles, 'Liberty measles?'...Remember when the hick legislators in certain states, in obedience to William Jennings Bryan, who learned his biology from his pious old grandma, set up shop as scientific experts and made the whole world laugh itself sick by forbidding the teaching of evolution? --Sinclair Lewis, It Can't Happen Here, 1935 From talulahgosh <@t> gmail.com Sat Sep 8 23:38:58 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Sat Sep 8 23:39:09 2007 Subject: [Histonet] Gloves for Microtomy In-Reply-To: <004401c7f20b$1c3277a0$0202a8c0@yourxhtr8hvc4p> References: <200709071844.l87Ii7Sr024319@mail-proofpoint-3a.usg.tufts.edu> <46E19F21.1060404@tufts.edu> <70EEF3D43B3C164C94037D811B2BE193E12CC8@VHAV20MSGA3.v20.med.va.gov> <003601c7f1ac$6f83b540$0202a8c0@yourxhtr8hvc4p> <70EEF3D43B3C164C94037D811B2BE193E12CCB@VHAV20MSGA3.v20.med.va.gov> <004401c7f20b$1c3277a0$0202a8c0@yourxhtr8hvc4p> Message-ID: Wow, all of these stories of human tissue makes me think the IACUC/DLAR (research animal committees/depts) are INCREDIBLY overreacting. however, i know there are rules and blah, blah, blah. It really makes me rethink the rules we follow using chick embryos (younger than 7 days)--they are considered food waste during most of their incubation time by the university, yet we follow specific rules in our lab. We wear gloves to prevent (RNase) contamination of the tissue, not contamination of ourselves! Better safe than sorry, I suppose (especially when it comes to university committees!) By the way, I've noticed most posters seem to be in pathology labs, are there any researchers in academics, as I am? Emily -- Remember our war hysteria, when we called sauerkraut 'Liberty cabbage' and somebody actually proposed calling German measles, 'Liberty measles?'...Remember when the hick legislators in certain states, in obedience to William Jennings Bryan, who learned his biology from his pious old grandma, set up shop as scientific experts and made the whole world laugh itself sick by forbidding the teaching of evolution? --Sinclair Lewis, It Can't Happen Here, 1935 From RSRICHMOND <@t> aol.com Sat Sep 8 23:49:50 2007 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Sat Sep 8 23:50:01 2007 Subject: [Histonet] Re: Gloves for Microtomy Message-ID: All this pathologist-bashing - much of it, alas, justifiable... I've never seen "stripping vessels" in the examination of lower extremity amputation specimens. The amount of information the pathologist can provide from these specimens is limited; in fact, some services do not have them examined at all. I like to look at sections of anterior tibial and gastrocnemius muscles, to demonstrate the ravages of diabetic neuropathy, but that's my personal mishegaas. I expect to take over the occasional yucky specimen from the pathologist's assistant (PA) - though - sorry about that - I do expect you to do the damn placentas. - Where is this legal-beagle notion coming from that we have to examine ALL placentas? This is something that needs to be worked out with the hospital's perinatal committee and with its obstetrical nursing service - the one time I ever was allowed to do this I worked it out quite well. To get back to the question that started this thread - never in my travels have I seen histotechnologists required to wear gloves for microtomy. - Wonder when the MBA's will have to wear rubber gloves?? Bob Richmond Samurai Pathologist Knoxville TN From JMacDonald <@t> mtsac.edu Sun Sep 9 01:57:13 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Sun Sep 9 01:57:24 2007 Subject: [Histonet] Continuing Education Opportunity S. CA Message-ID: McCormick Scientific in conjunction with Mount San Antonio College ? School of Histotechnology Presents LUNCH & LEARN SERIES A Continuing Education Forum in Histotechnology & Lab Management 3 Speakers Continuing Education Credit Complimentary Lunch Lectures Include: Dynamics of Fixation & Processing Diagnostic Immunohistochemistry in Breast Cancer Studies Reprocessing Breast Tissue ? Cost & Workload Value A Family Affair ? A Systems Approach to Team Development Hosted by: Mt. San Antonio College School of Histotechnology 1100 N. Grand Avenue Walnut, California 91789 Saturday, September 29, 2007 9:00 a.m. ? 2:00 p.m. R.S.V.P. For additional information contact: Jennifer MacDonald, Program Director 909-594-5611 ext. 4884 jmacdonald@mtsac.eduu From moran.elish <@t> gmail.com Sun Sep 9 09:01:10 2007 From: moran.elish <@t> gmail.com (Moran Elishmereni) Date: Sun Sep 9 09:01:26 2007 Subject: [Histonet] toluidine staining faded! Message-ID: <4a722ef70709090701j42b16bc3k46c3218d022fc3a8@mail.gmail.com> Hello histonetters, I stained some murine skin sections for toluidine blue, and mast cells were very easy to detect, however, after a few days the staining seemed to have faded. Following the tol blue staining, I briefly put the sections in alcohol (95%, 100%, 100%), then in xylene substitute, then air dryed and mounted with mounting media. What did I do wrong? I am very new at histology, so forgive my simple question... Thanks, Moran -- Moran Elishmereni Department of Pharmacology and Experimental Therapeutics School of Pharmacy, Faculty of Medicine The Hebrew University of Jerusalem POB 12065 Jerusalem 91120, ISRAEL Tel: 972-2-675-8746 Fax: 972-2-675-8144 Email: moran.elish@gmail.com From Jason.Burrill <@t> crl.com Sun Sep 9 09:06:41 2007 From: Jason.Burrill <@t> crl.com (Burrill, Jason) Date: Sun Sep 9 09:09:37 2007 Subject: [Histonet] Re: Gloves for Microtomy Message-ID: <1AD4E907E9B6F648AEF1B3A20A9B0E1E0BDE6B@shr-exch2.na01.crl.com> Thanks Joe and I can remember that while attending Histology school at AFIP they said "It 's useless for you to wear gloves while coverslipping because they just melt in the Xylene so just use your bare hands". Granted that we did not have access to Nitrile gloves in school, we only used latex, but we also did it on the counter with no ventilated workspace. Although this was 12 years ago, I am pretty sure that the Lab Standard would have still applied since it went into affect in 1990. Emily I am also in a research/diagnostic veterinary setting and act as the Lab Safety liaison for questions regarding the safe handling/disposal all chemicals and biological samples for not only the histology lab but also for Microbiology, Molecular Biology, Necropsy and Serology. Although you probably already know, I would assume that you are wearing gloves because the tissues that are collected are being sent for molecular diagnostic analysis and the gloves are used to prevent cross contamination, I also assume that you are spraying your gloves with RNase Away. Although it is not the reason you are wearing gloves, their use for biosecurity reasons is a common practice in research institutions for handling animals that have little to no immune system because those types of animals are highly susceptible to contracting diseases from common bacteria and fungus. If animals are exposed to certain microorganisms it can be a nightmare for the animal colony health if animals are housed together. Hope this helped. Jason Burrill Manager, Histology Charles River Laboratories 251 Ballardvale St Wilmington, MA 01887 Phone: 978-658-6000 ext. 1652 Fax: 978-988-8793 jason.burrill@crl.com From rjbuesa <@t> yahoo.com Sun Sep 9 09:29:53 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Sep 9 09:30:08 2007 Subject: [Histonet] toluidine staining faded! In-Reply-To: <4a722ef70709090701j42b16bc3k46c3218d022fc3a8@mail.gmail.com> Message-ID: <862255.57038.qm@web61223.mail.yahoo.com> Moran: My first concern is: if you dehydrated and placed in xylene substitute, WHY did you air dried them? After you place them in xylene substitute you can coverslip them UNLESS your mounting medium is incompatible wit the xylene substitute, in which case you should not use that substitute or change to another mounting medium. Toluidine blue fading can be cause by low pH and the cause could be the mounting medium that is not neutral (pH 7). Ren? J. Moran Elishmereni wrote: Hello histonetters, I stained some murine skin sections for toluidine blue, and mast cells were very easy to detect, however, after a few days the staining seemed to have faded. Following the tol blue staining, I briefly put the sections in alcohol (95%, 100%, 100%), then in xylene substitute, then air dryed and mounted with mounting media. What did I do wrong? I am very new at histology, so forgive my simple question... Thanks, Moran -- Moran Elishmereni Department of Pharmacology and Experimental Therapeutics School of Pharmacy, Faculty of Medicine The Hebrew University of Jerusalem POB 12065 Jerusalem 91120, ISRAEL Tel: 972-2-675-8746 Fax: 972-2-675-8144 Email: moran.elish@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Pinpoint customers who are looking for what you sell. From carl.hobbs <@t> kcl.ac.uk Sun Sep 9 12:21:52 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Sun Sep 9 12:22:17 2007 Subject: [Histonet] Re: gloves Message-ID: <003f01c7f305$e9db06e0$4101a8c0@carlba65530bda> Jason.... Damn fine answer. Respect! Carl From jkiernan <@t> uwo.ca Sun Sep 9 17:07:16 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Sun Sep 9 17:07:32 2007 Subject: [Histonet] toluidine staining faded! In-Reply-To: <4a722ef70709090701j42b16bc3k46c3218d022fc3a8@mail.gmail.com> References: <4a722ef70709090701j42b16bc3k46c3218d022fc3a8@mail.gmail.com> Message-ID: Air drying before mounting is wrong because you get innumerable tiny bubbles within the section, which are held in place by the resinous mounting medium. The section looks grey and opaque. The slide must be wet with xylene before you apply the mounting medium and coverslip. You can often air-dry sections instead of dehydrating with alcohol, but xylene (or equivalent) before coverslipping is a must. John Kiernan Anatomy, UWO London, Canada --- ----- Original Message ----- From: Moran Elishmereni Date: Sunday, September 9, 2007 10:02 Subject: [Histonet] toluidine staining faded! To: histonet@lists.utsouthwestern.edu > Hello histonetters, > > I stained some murine skin sections for toluidine blue, and mast > cells were > very easy to detect, however, after a few days the staining > seemed to have > faded. Following the tol blue staining, I briefly put the > sections in > alcohol (95%, 100%, 100%), then in xylene substitute, then air > dryed and > mounted with mounting media. What did I do wrong? I am very new > at histology, so forgive my simple question... > > Thanks, > Moran > > > -- > Moran Elishmereni > > Department of Pharmacology and Experimental Therapeutics > School of Pharmacy, Faculty of Medicine > The Hebrew University of Jerusalem > POB 12065 > Jerusalem 91120, ISRAEL > Tel: 972-2-675-8746 > Fax: 972-2-675-8144 > Email: moran.elish@gmail.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jnocito <@t> satx.rr.com Sun Sep 9 18:42:43 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Sun Sep 9 18:43:06 2007 Subject: [Histonet] protocol for clearing tissues References: Message-ID: <005801c7f33b$1e5ab6b0$0202a8c0@yourxhtr8hvc4p> is like today with Freedom Fries, Freedom Wine and Freedom Kissing? ----- Original Message ----- From: "Emily Sours" To: Sent: Saturday, September 08, 2007 11:19 PM Subject: Re: [Histonet] protocol for clearing tissues > BABB clears our whole mount IHC very well, but we've noticed it gets > everywhere and dissolves everything we use to view embryos, so we've moved > to glass. > It's also hard to wash away since it's insoluble in water and alcohol--at > least that's our experience. > > Emily > -- > Remember our war hysteria, when we called sauerkraut 'Liberty cabbage' and > somebody actually proposed calling German measles, 'Liberty > measles?'...Remember when the hick legislators in certain states, in > obedience to William Jennings Bryan, who learned his biology from his > pious > old grandma, set up shop as scientific experts and made the whole world > laugh itself sick by forbidding the teaching of evolution? > --Sinclair Lewis, It Can't Happen Here, 1935 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anthony <@t> histotechexchange.com Sun Sep 9 19:36:46 2007 From: anthony <@t> histotechexchange.com (anthony@histotechexchange.com) Date: Sun Sep 9 19:37:28 2007 Subject: [Histonet] Histology Position in St. Louis, Missouri Message-ID: <4432.65.196.168.131.1189384629.squirrel@host4.wfdns.com> Reference lab needs accurate histologist. Good cutting and embedding skills required for this 13 week contract position (with the possibility of an extension or a full-time position) in a beautiful, vibrant suburb of St Louis, MO. All travel expenses paid; good pay, including expenses. Shift starts 7:30 a.m. from Monday to Friday. If you care about the patient, have over two years of experience and can get on with others, please call or email. Thank you, Anthony Williams Histotech Exchange LLC 19 Whitmore Street Lexington, Virginia 24450 anthony@histotechexchange.com 1-877-464-8911 From cabramley <@t> students.latrobe.edu.au Sun Sep 9 21:47:15 2007 From: cabramley <@t> students.latrobe.edu.au (CLAIRE ANN KENTLER) Date: Sun Sep 9 21:47:29 2007 Subject: [Histonet] OCT Compound Message-ID: <91DC98B386D8614487170C699BE9299F04FE610F@stexchange1.students.ltu.edu.au> Hi all, Just to say thanks so much to everyone for your advice, (and for those who offered to send me some!) I managed to get hold of the supplier and will be able to pick some up from them this afternoon, but shall keep all the advice in mind for next time... Thanks so much, Claire ************************************************************************ *************************************** Claire Kentler Bachelor of Animal Science (Hons) Postgraduate Student Department of Agricultural Sciences La Trobe University Phone: 9479 1048 E-mail: cabramley@students.latrobe.edu.au From moran.elish <@t> gmail.com Mon Sep 10 01:28:41 2007 From: moran.elish <@t> gmail.com (Moran Elishmereni) Date: Mon Sep 10 01:28:55 2007 Subject: [Histonet] which mounting media after Neo-Clear? Message-ID: <4a722ef70709092328l74f41132w2361e0c1e0b5f9f5@mail.gmail.com> Hi everyone, Does anyone know which simple mounting media I can apply after using Neo-Clear (a xylene substitute) on paraffin-embedded slides that had been stained with toluidine blue? I am looking for a solution other than "Neo-Clear mounting media". And thanks for all the responses regarding the toluidine blue fading issue... Thanks, Moran Elishmereni Department of Pharmacology and Experimental Therapeutics School of Pharmacy, Faculty of Medicine The Hebrew University of Jerusalem POB 12065 Jerusalem 91120, ISRAEL Tel: 972-2-675-8746 Fax: 972-2-675-8144 Email: moran.elish@gmail.com From Kemlo.Rogerson <@t> waht.swest.nhs.uk Mon Sep 10 02:34:06 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Sep 10 02:34:20 2007 Subject: [Histonet] Re: Gloves Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222EC99@wahtntex2.waht.swest.nhs.uk> I just graduated from a Nccls program through Mayo/UND and we were not taught to cut wearing gloves, in fact there is only one tech in the lab that consistently wears gloves while cutting and she came to us from the enzyme histochemistry lab so she couldn't get used to not wearing gloves. As far as the mesh gloves, we have those also and are supposed to wear them whenever changing/handling blades per our safety officer because right after I started there was a rash of people cutting themselves. Laurie As one who literally bears the scars of microtomy we used not to think ourselves as 'men' unless we went to A&E occasionally to get a cut sutured. The most painful cut were those you got from using a knife back and a wooden stick laden with diamond paste. The idea was to draw the stick across the knife blade with you finger acting as guide, sometimes it slipped off the stick (diamond paste and blood makes a curious mix). The big Tetrander knives were frightening as you could loose a whole body part! These 'new' blades that are disposable only give you a 'love bite' that you soon grow out of and never remember, the loss of one of your finger ends captures your attention a tad. Must point out though these 'new' knives are sharper, safer and produce better sections, that is as long as the tissue isn't hard. Progress I guess but what do people do when they are not sharpening knives? ICC I guess! Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; When your fear touches someone's pain, it becomes pity. When your love touches someone's pain, it becomes compassion. --Stephen Levine This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Susan.Walzer <@t> HCAHealthcare.com Mon Sep 10 02:40:04 2007 From: Susan.Walzer <@t> HCAHealthcare.com (Walzer Susan) Date: Mon Sep 10 02:40:17 2007 Subject: [Histonet] Gloves for Microtomy In-Reply-To: Message-ID: <471953BC63077941B82C26A4338272B42F0538@ORLEV03.hca.corpad.net> Gloves have NEVER been required to cut paraffin blocks. Gloves would more than likely be the cause of accidents as they would get in the way cutting. Has anyone ever tried to change the film in a camera with gloves on? Doesn't work... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Bill Sent: Friday, September 07, 2007 3:14 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Gloves for Microtomy I have formally placed lab inspectors in the same category as lawyers, regulators and politicians. m|m At 12:57 PM -0500 9/7/07, Dawson, Glen wrote: >I have a rogue JCAHO inspector currently on-site that is demanding >that all of the histotechs cut paraffin blocks with gloves on. She >has made up many other rules of her own but most were so outlandish >that they were easily discounted. -- _____________________________ Bill Blank http://kernunnos.com (Celtic studies and numismatics) OBOD's Message board: http://www.druidry.org/board/dhp/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Mon Sep 10 08:23:43 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Mon Sep 10 08:26:58 2007 Subject: [Histonet] toluidine staining faded! In-Reply-To: <4a722ef70709090701j42b16bc3k46c3218d022fc3a8@mail.gmail.com> References: <4a722ef70709090701j42b16bc3k46c3218d022fc3a8@mail.gmail.com> Message-ID: <46E5455F.8000906@umdnj.edu> I suggest that you dehydrate with 2 changes of acetone, then clear in fresh xylene and coverslip. Geoff Moran Elishmereni wrote: > Hello histonetters, > > I stained some murine skin sections for toluidine blue, and mast cells were > very easy to detect, however, after a few days the staining seemed to have > faded. Following the tol blue staining, I briefly put the sections in > alcohol (95%, 100%, 100%), then in xylene substitute, then air dryed and > mounted with mounting media. What did I do wrong? I am very new > at histology, so forgive my simple question... > > Thanks, > Moran > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From LINDA.MARGRAF <@t> childrens.com Mon Sep 10 09:10:19 2007 From: LINDA.MARGRAF <@t> childrens.com (LINDA MARGRAF) Date: Mon Sep 10 09:11:38 2007 Subject: [Histonet] Histonet replies Message-ID: <46E509FB020000DA00013339@CNET3.CHILDRENS.COM> Dear Histonetters: I just wanted to remind people that when they reply to a message that they are viewing in digest mode, to please not include the entire digest in their message. Sometime the message will be so large, the server will block it. If it goes through, it will cause everyone reading the digest to get lots of messages several times. Please cut and paste the single message into your reply if you wish to include it in your response. Thanks so much, Linda M Histonet administrator PS The Histonet list is up to 2777 members! Thanks so much for contributing. From Terry.Marshall <@t> rothgen.nhs.uk Mon Sep 10 09:40:27 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Mon Sep 10 10:01:02 2007 Subject: [Histonet] Gloves for Microtomy Message-ID: <407F05A128805F4C879A33DBA32E618E0189503C@TRFT-EX01.xRothGen.nhs.uk> That about says it all and succinctly. Totally absurd idea. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Walzer Susan Sent: 10 September 2007 08:40 To: Bill; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Gloves for Microtomy Gloves have NEVER been required to cut paraffin blocks. Gloves would more than likely be the cause of accidents as they would get in the way cutting. Has anyone ever tried to change the film in a camera with gloves on? Doesn't work... Susan Walzer From eschurga <@t> aerotek.com Mon Sep 10 10:07:21 2007 From: eschurga <@t> aerotek.com (Schurga, Erich) Date: Mon Sep 10 10:07:45 2007 Subject: [Histonet] Histotechnologist Positions in Central Florida Message-ID: Good Morning, I'm looking for five Histotechnologist for an established lab in Central Florida. Attached below is the job description. Starting pay is between $26/hr - $34/hr, based on experience. Please contact me directly if you would like more information, or if you know of any Histotechnologists looking for work. My client is looking to hire immediately and these are all direct placement positions. Histotechnologist Job Description: An established Laboratory in Altamonte Springs, FL is currently seeking five Histotechnologist, entry level to experienced. This is a direct placement job, with a salary range of $26 - $34/hr. based on experience. We offer competitive salaries; health, dental and disability insurance; paid holidays; paid vacations; and 401(k) retirement plan. Responsibilities consist of all elements of biopsy processing and staining for diagnosis. Precisely and accurately performs a variety of routine and specialized histology techniques and procedures. Meets embedding and sectioning productivity standards. Qualified Histotechnologists will have: - Training and certification as HT or HLT by ASCP - Florida Licensure - AS from accredited school in Histology or related science. Erich J. Schurga Scientific Recruiter Aerotek, Inc. * Phone: 321.354.1057 * Fax: 321.354.1080 * Toll Free: 888.455.1329 * E-mail: eschurga@aerotek.com 3660 Maguire Blvd. Suite 107 Orlando, FL 32803-3059 Click here to search for open positions: www.aerotek.com ____________________________________________________________________________________________________ This electronic mail (including any attachments) may contain information that is privileged, confidential, and/or otherwise protected from disclosure to anyone other than its intended recipient(s). Any dissemination or use of this electronic email or its contents (including any attachments) by persons other than the intended recipient(s) is strictly prohibited. If you have received this message in error, please notify us immediately by reply email so that we may correct our internal records. Please then delete the original message (including any attachments) in its entirety. Thank you. From cjbulmer <@t> sbcglobal.net Mon Sep 10 11:30:43 2007 From: cjbulmer <@t> sbcglobal.net (Cindy Bulmer) Date: Mon Sep 10 11:30:57 2007 Subject: [Histonet] IHC position available Message-ID: <975309.49408.qm@web82309.mail.mud.yahoo.com> Central Texas Pathology Laboratory in Waco, TX has an immediate position available for an IHC technician. Must be HT(ASCP) registered and prefer some IHC experience. CTPL offer great benefits along with competitive salary. Please contact: Cynthia Bulmer, HT(ASCP)QIHC IHC Supervisor, CTPL Waco, TX 254-752-9621 ext.247 From slappycraw <@t> yahoo.com Mon Sep 10 11:36:13 2007 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Mon Sep 10 11:36:25 2007 Subject: [Histonet] Gloves for Microtomy Message-ID: <697327.72988.qm@web53601.mail.re2.yahoo.com> If this kind of hysteria goes unchecked we will soon be wearing full on hazmat suits just to be in the lab. I think something similar happens to people that go into politics and safety. --------------------------------- Ready for the edge of your seat? Check out tonight's top picks on Yahoo! TV. From Shirley_PHUA <@t> hsa.gov.sg Mon Sep 10 13:02:19 2007 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Mon Sep 10 13:08:54 2007 Subject: [Histonet] Shirley Phua is away on 11 & 12 Sep 2007 (Tuesday & Wednesday). Message-ID: I will be out of the office from 11-09-2007 to 12-09-2007. I'll be away on 11 & 12 Sep 2007 (Tuesday & Wednesday). I'll be back on 13 Sep 2007 (Thursday). Pathologists: I will process your requests when I return. If urgent, please forward your email to Henry_Kyaw@hsa.gov.sg From carl.hobbs <@t> kcl.ac.uk Mon Sep 10 14:58:52 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Mon Sep 10 14:59:17 2007 Subject: [Histonet] Re: cleaved caspase(active) Message-ID: <003f01c7f3e5$032a9090$4101a8c0@carlba65530bda> A final question, after a preamble: I use Cell Signalling's, Biocare's and Abcam's anti Cleaved caspase 3 ( "active" : I always add that bit in, just in case;-), on pwax sections after Citric acid HIER pH6 ( no other retrieval method/nor omission works, for me ) For me, the first Ab is too variable; works very well, sometimes. The second works most of the time, except that I am worried that the number of positive cells makes me think that non-cleaved caspase 3 is demonstrated. ( in serial sections, Biocare's Ab gave nuclear positivity that was absent, repeatedly, with the other two, when I judged them to be working well ....there appeared to be a worrying co-positivity with Ki67....is this possible?) The third Ab tends to be consistent BUT , again, demonstrates less cells than the Biocare Ab. As does the Cell Signalling Ab, when it works. Final Q: Finally....has anyone actually compared the available anti CC3 ( active ;-)Abs to check for fidelity???? Any comments on these comments of mine would be gratefully recd. Yep..the only consistency in my comments is inconsistency! Curious Carl From ccross6032 <@t> aol.com Mon Sep 10 16:06:15 2007 From: ccross6032 <@t> aol.com (Cheryl Cross) Date: Mon Sep 10 16:06:35 2007 Subject: [Histonet] Re: cleaved caspase(active) In-Reply-To: <003f01c7f3e5$032a9090$4101a8c0@carlba65530bda> References: <003f01c7f3e5$032a9090$4101a8c0@carlba65530bda> Message-ID: Hi Carl - Not sure I can be of any help...but one thing I've found (and one reason I trust Cell Signaling's ab) is that I use thymus as my positive control and I get nice staining in the thymic (and lymph node) medulla in cells that often have a shrunken, dense nucleus (ie look microscopically apoptotic). When I tried another antibody positive staining was "willy nilly" if you will - endothelial cells, scattered circulating leukocytes, and then many many lymphocytes within the thymic cortex and medulla - i just did not trust that all those cells were apoptotic in a healthy control! CC Cheryl Cross, DVM, Dipl. ACVP Researcher University Corporation for Atmospheric Research College of Veterinary Medicine University of Tennessee Department of Pathology 2407 River Drive, Room A201 Knoxville, TN 37996-4542 (423) 967-2724 fax: 865-974-5616 ccross@ucar.edu On Sep 10, 2007, at 3:58 PM, Carl Hobbs wrote: > A final question, after a preamble: > > > I use Cell Signalling's, Biocare's and Abcam's anti Cleaved caspase > 3 ( "active" : I always add that bit in, just in case;-), on pwax > sections after Citric acid HIER pH6 ( no other retrieval method/nor > omission works, for me ) > > For me, the first Ab is too variable; works very well, sometimes. > The second works most of the time, except that I am worried that > the number of positive cells makes me think that non-cleaved > caspase 3 is demonstrated. ( in serial sections, Biocare's Ab gave > nuclear positivity that was absent, repeatedly, with the other two, > when I judged them to be working well > ....there appeared to be a worrying co-positivity with Ki67....is > this possible?) > The third Ab tends to be consistent BUT , again, demonstrates less > cells than the Biocare Ab. As does the Cell Signalling Ab, when it > works. > Final Q: Finally....has anyone actually compared the available anti > CC3 ( active ;-)Abs to check for fidelity???? > Any comments on these comments of mine would be gratefully recd. > Yep..the only consistency in my comments is inconsistency! > Curious Carl > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Mon Sep 10 17:57:31 2007 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Sep 10 17:58:24 2007 Subject: [Histonet] Gloves for Microtomy Message-ID: It is not impossible to change film with gloves on. In our Electron Microscopy Unit we have to load 5x4 film (single sheets) in the dark wearing cotton gloves (stops greese from contaminating the column - one hopes!). It takes practice. I only wear gloves when I have to cut thick paraffin sections for RNA and DNA extractions, which we seem to be on the increase. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Walzer Susan Sent: Monday, 10 September 2007 5:40 PM To: Bill; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Gloves for Microtomy Gloves have NEVER been required to cut paraffin blocks. Gloves would more than likely be the cause of accidents as they would get in the way cutting. Has anyone ever tried to change the film in a camera with gloves on? Doesn't work... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Bill Sent: Friday, September 07, 2007 3:14 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Gloves for Microtomy I have formally placed lab inspectors in the same category as lawyers, regulators and politicians. m|m At 12:57 PM -0500 9/7/07, Dawson, Glen wrote: >I have a rogue JCAHO inspector currently on-site that is demanding >that all of the histotechs cut paraffin blocks with gloves on. She >has made up many other rules of her own but most were so outlandish >that they were easily discounted. -- _____________________________ Bill Blank http://kernunnos.com (Celtic studies and numismatics) OBOD's Message board: http://www.druidry.org/board/dhp/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Mon Sep 10 18:14:02 2007 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Sep 10 18:14:51 2007 Subject: [Histonet] Gloves for Microtomy Message-ID: Sorry, my spelling is atrocious and what about the double negatives! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Tuesday, 11 September 2007 8:58 AM To: Walzer Susan; Bill; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Gloves for Microtomy It is not impossible to change film with gloves on. In our Electron Microscopy Unit we have to load 5x4 film (single sheets) in the dark wearing cotton gloves (stops greese from contaminating the column - one hopes!). It takes practice. I only wear gloves when I have to cut thick paraffin sections for RNA and DNA extractions, which we seem to be on the increase. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Walzer Susan Sent: Monday, 10 September 2007 5:40 PM To: Bill; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Gloves for Microtomy Gloves have NEVER been required to cut paraffin blocks. Gloves would more than likely be the cause of accidents as they would get in the way cutting. Has anyone ever tried to change the film in a camera with gloves on? Doesn't work... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Bill Sent: Friday, September 07, 2007 3:14 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Gloves for Microtomy I have formally placed lab inspectors in the same category as lawyers, regulators and politicians. m|m At 12:57 PM -0500 9/7/07, Dawson, Glen wrote: >I have a rogue JCAHO inspector currently on-site that is demanding that >all of the histotechs cut paraffin blocks with gloves on. She has made >up many other rules of her own but most were so outlandish that they >were easily discounted. -- _____________________________ Bill Blank http://kernunnos.com (Celtic studies and numismatics) OBOD's Message board: http://www.druidry.org/board/dhp/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From Gervaip <@t> aol.com Mon Sep 10 21:43:17 2007 From: Gervaip <@t> aol.com (Gervaip@aol.com) Date: Mon Sep 10 21:43:32 2007 Subject: [Histonet] week end diff Message-ID: ************************************** See what's new at http://www.aol.com -------------- next part -------------- Hi, in the past, post Katrina, the histology folk always got a week end N but no w survey to end diff along with th lab assistants. Yes, the usual, the histology folks are wonder why it is so hard to get a goo Pearl Gervais, New Orleans From conniegrubaugh <@t> hotmail.com Mon Sep 10 22:00:56 2007 From: conniegrubaugh <@t> hotmail.com (connie grubaugh) Date: Mon Sep 10 22:01:12 2007 Subject: [Histonet] chucks for old cyostat Message-ID: I'm looking for chucks that have just a stem on the bottom to attach to the holder for a old cryostat. Any help will be appreciated. Connie G. _________________________________________________________________ Kick back and relax with hot games and cool activities at the Messenger Caf?. http://www.cafemessenger.com?ocid=TXT_TAGLM_SeptWLtagline From abright <@t> brightinstruments.com Tue Sep 11 05:45:47 2007 From: abright <@t> brightinstruments.com (Alan Bright) Date: Tue Sep 11 06:14:36 2007 Subject: [Histonet] chucks for old cyostat References: Message-ID: Dear Connie, Please email me a sketch with dimensions, I am sure if it does not fall into our range of object holders we will be able to make whatever. Best Regards Alan Bright Bright Instrument Co.Ltd. Cryostat & Microtome Divn. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com Skype: dazzle0 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of connie grubaugh Sent: 11 September 2007 04:01 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] chucks for old cyostat I'm looking for chucks that have just a stem on the bottom to attach to the holder for a old cryostat. Any help will be appreciated. Connie G. _________________________________________________________________ Kick back and relax with hot games and cool activities at the Messenger Caf?. http://www.cafemessenger.com?ocid=TXT_TAGLM_SeptWLtagline_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ancillarypath <@t> mac.com Tue Sep 11 06:39:28 2007 From: ancillarypath <@t> mac.com (ancillarypath@mac.com) Date: Tue Sep 11 06:39:42 2007 Subject: [Histonet] Second Annual Course of Applied IHC and Molecular Morphology In-Reply-To: <200709101706.l8AH6otQ013108@mac.com> References: <200709101706.l8AH6otQ013108@mac.com> Message-ID: Dear colleagues, This is Hadi Yaziji. It is my pleasure to announce the open registration for the second annual course of applied IHC and molecular morphology, sponsored by the Society of Applied Immunohistochemistry. This is held in Santa Barbara from January 28 - February 1st, 2008. It contains the following: 26 lectures by the most experienced pathologists and technologists in immunohistochemistry, FISH and flow cytometry. Panel discussion by the leaders in the industry Panel discussion by the founders of the field (Battifora, Taylor, Gown) A workshop on basic and complex IHC procedures A workshop on FISH procedures Please click on the link to download a pdf version of the course's brochure (http://www.pathlearning.com/IHC_Course08.pdf). If you intend to register, please do so as soon as you can. Space is really limited at the resort. Registration and course information are in the brochure. Best regards, ================================ Hadi Yaziji, M.D., Medical Director Vitro Molecular Laboratories President, Ancillary Pathways 7000 62nd Avenue, PH-C Miami, FL 33143 T 305-740-4440 F. 786-513-0175 www.vitromolecular.com www.ancillarypath.com From relia1 <@t> earthlink.net Tue Sep 11 06:49:41 2007 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Sep 11 06:49:55 2007 Subject: [Histonet] RELIA Histology Careers Bulletin 9-10-07 Let's Get the Ball Rolling!!! Message-ID: Hi Histonetters! It is hard to believe the summer is over already. Before we know it, it will be 2008! Let?s Get The Ball Rolling!!! Looking for a new job in the Fall? Planning a job change after the holidays? Considering making a move in 2008? Are you a histo tech looking for a better opportunity? Are you a new or recent graduate of a histology school? Are you a traveler considering transitioning into a permanent position? Are you a Histo Tech looking for an opportunity to advance into management? Let?s Get The Ball Rolling!!! I can put RELIA?s 5 Point Career Mapping Strategy to Work For You. Here?s how it works: *R*E*L*I*A* ? *Resume Fine Tuning ? *Economic Analysis ?*Laser in on your dream job ? *Interview Coaching ? *Arrangements and Timeframes Customized to you Convenience All you have to do is shoot me an e-mail at relia1@earthlink.net or give me a call toll free at 866-607-3542 my office is open M-F 7am-8pm EST or by appointment. Here is some information about my current openings: Histology Managers/Supervisors needed in: Dallas, TX Santa Barbara, CA Valencia, CA Cape Cod, MA Chicago, IL Histotechnicians/Histotechnologists needed in: New York, NY Atlanta, GA San Francisco, CA Los Angeles, CA Valencia, CA Los Angeles, CA ? oppty to learn MOHS Orlando, FL St. Petersburg, FL Fredericksburg, VA Dallas, TX Waco, TX Pittsburgh, PA Columbus, OH If you know of anyone else who might be interested in hearing about RELIA?s Career Mapping Strategy or would like to subscribe to RELIA?s Histology Careers Bulletin please feel free to pass this e-mail along to them. Remember it never hurts to look!!! Thanks, Pam 877-60 RELIA (877-607-3542) Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net From eschurga <@t> aerotek.com Tue Sep 11 07:27:11 2007 From: eschurga <@t> aerotek.com (Schurga, Erich) Date: Tue Sep 11 07:27:27 2007 Subject: [Histonet] FL Licensure Message-ID: Hello, I'm trying to find out more information about Florida Licensure. My client requires all candidates to have a FL License or a temporary permit to work at a Histotechnologist level. I have had candidates apply to my position from out of state. Where can I find more information about obtaining a temporary permit in the state of Florida? Thanks in advance for the help. Erich J. Schurga Scientific Recruiter Aerotek, Inc. * Phone: 321.354.1057 * Fax: 321.354.1080 * Toll Free: 888.455.1329 * E-mail: eschurga@aerotek.com 3660 Maguire Blvd. Suite 107 Orlando, FL 32803-3059 Click here to search for open positions: www.aerotek.com ____________________________________________________________________________________________________ This electronic mail (including any attachments) may contain information that is privileged, confidential, and/or otherwise protected from disclosure to anyone other than its intended recipient(s). Any dissemination or use of this electronic email or its contents (including any attachments) by persons other than the intended recipient(s) is strictly prohibited. If you have received this message in error, please notify us immediately by reply email so that we may correct our internal records. Please then delete the original message (including any attachments) in its entirety. Thank you. From rjbuesa <@t> yahoo.com Tue Sep 11 07:40:11 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Sep 11 07:40:22 2007 Subject: [Histonet] FL Licensure In-Reply-To: Message-ID: <164541.34711.qm@web61221.mail.yahoo.com> Erich: Go to: http://www.fshgroup.org and you will find the information you need Ren? J. "Schurga, Erich" wrote: Hello, I'm trying to find out more information about Florida Licensure. My client requires all candidates to have a FL License or a temporary permit to work at a Histotechnologist level. I have had candidates apply to my position from out of state. Where can I find more information about obtaining a temporary permit in the state of Florida? Thanks in advance for the help. Erich J. Schurga Scientific Recruiter Aerotek, Inc. * Phone: 321.354.1057 * Fax: 321.354.1080 * Toll Free: 888.455.1329 * E-mail: eschurga@aerotek.com 3660 Maguire Blvd. Suite 107 Orlando, FL 32803-3059 Click here to search for open positions: www.aerotek.com /0314148114.jpg> ____________________________________________________________________________________________________ This electronic mail (including any attachments) may contain information that is privileged, confidential, and/or otherwise protected from disclosure to anyone other than its intended recipient(s). Any dissemination or use of this electronic email or its contents (including any attachments) by persons other than the intended recipient(s) is strictly prohibited. If you have received this message in error, please notify us immediately by reply email so that we may correct our internal records. Please then delete the original message (including any attachments) in its entirety. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Luggage? GPS? Comic books? Check out fitting gifts for grads at Yahoo! Search. From thicks <@t> gulfcoastdermpath.com Tue Sep 11 07:47:47 2007 From: thicks <@t> gulfcoastdermpath.com (Trish Hicks) Date: Tue Sep 11 07:48:17 2007 Subject: [Histonet] Positions Available Message-ID: <730AEE77975BE74B87C7FB31A38C7BE963000D@exbe01.connectwise.hosted> Gulf Coast Dermatopathology, in sunny Tampa, Florida currently has two positions available. There is a night shift position and a day shift position available. Gulf Coast offers competitive salaries and benefits. Gulf Coast is a small to medium sized laboratory which is growing at a constant pace and has been in business for many years. We are looking for individuals who are highly motivated, self sufficient and who are focused on quality and accuracy. A candidate must be a licensed Florida Histotechnologist or Histotechnician. ASCP certification is a plus. New grads and experienced techs welcome. A working knowledge of dermatopathology is extremely helpful. For further information, contact Trish Hicks or Sharon Miller at 813-882-4206 OR E-mail: thicks@gulfcoastdermpath.com or smiller@gulfcoastdermpath.com From mpence <@t> grhs.net Tue Sep 11 08:04:46 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Sep 11 08:05:02 2007 Subject: [Histonet] week end diff In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C70F@IS-E2K3.grhs.net> My staff gets shift diff and weekend diff Mike Pence AP Supervisor Great River Medical Center West Burlington, IA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gervaip@aol.com Sent: Monday, September 10, 2007 9:43 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] week end diff ************************************** See what's new at http://www.aol.com From ree3 <@t> leicester.ac.uk Tue Sep 11 08:21:05 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Tue Sep 11 08:21:23 2007 Subject: [Histonet] week end diff In-Reply-To: <661949901A768E4F9CC16D8AF8F2838CA1C70F@IS-E2K3.grhs.net> References: <661949901A768E4F9CC16D8AF8F2838CA1C70F@IS-E2K3.grhs.net> Message-ID: But does the differential count?. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: 11 September 2007 14:05 To: Gervaip@aol.com; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] week end diff My staff gets shift diff and weekend diff Mike Pence AP Supervisor Great River Medical Center West Burlington, IA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gervaip@aol.com Sent: Monday, September 10, 2007 9:43 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] week end diff ************************************** See what's new at http://www.aol.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Tue Sep 11 11:49:19 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue Sep 11 10:52:06 2007 Subject: [Histonet] Worlds Largest Paraffin Dispenser Message-ID: I am looking for a paraffin dispenser that holds a LARGE amount of paraffin. My requests for a hot tub that I can fill with paraffin have been denied for some reason. Anyone know of a manufacturer of these large dispensers? Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. From cmiller <@t> physlab.com Tue Sep 11 11:11:30 2007 From: cmiller <@t> physlab.com (Cheri Miller) Date: Tue Sep 11 11:11:45 2007 Subject: [Histonet] week end diff In-Reply-To: References: Message-ID: <004f01c7f48e$6cc14f90$3402a8c0@plab.local> We not only get weekend diff, we also get shift diff. I assumed everyone did. Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gervaip@aol.com Sent: Monday, September 10, 2007 9:43 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] week end diff ************************************** See what's new at http://www.aol.com PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From rjbuesa <@t> yahoo.com Tue Sep 11 11:14:38 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Sep 11 11:14:51 2007 Subject: [Histonet] week end diff In-Reply-To: <004f01c7f48e$6cc14f90$3402a8c0@plab.local> Message-ID: <897156.77347.qm@web61213.mail.yahoo.com> Shift differential is more "customary", but week-end differential is not; usually week-ends are included on a rotation basis, to complete the 40 hours/week Ren? J. Cheri Miller wrote: We not only get weekend diff, we also get shift diff. I assumed everyone did. Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gervaip@aol.com Sent: Monday, September 10, 2007 9:43 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] week end diff ************************************** See what's new at http://www.aol.com PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Got a little couch potato? Check out fun summer activities for kids. From robinsoc <@t> mercyhealth.com Tue Sep 11 11:23:31 2007 From: robinsoc <@t> mercyhealth.com (Cindy Robinson) Date: Tue Sep 11 11:24:18 2007 Subject: [Histonet] Lymph node fixative/enhance solutions In-Reply-To: <6AF8653B3VO602000-01@EMF2> References: <6AF8653B3VO602000-01@EMF2> Message-ID: <46E67AB3.59AC.00AF.0@mercyhealth.com> Our pathologists would like us to start using a solution to help with the dissection and ID of lymph nodes at gross. I have done some searching thru the archives and found some recipes but would like to find a commercial source. We do use Pen-Fix but it doesn't dissolve the fat like the paths would like. Has anyone got a source or recipe for a fat dissolving solution that they would like to share? Thanks. Cindi Robinson, HT (ASCP) Dunes Medical Laboratories Dakota Dunes SD 57049 From DDittus787 <@t> aol.com Tue Sep 11 11:33:20 2007 From: DDittus787 <@t> aol.com (DDittus787@aol.com) Date: Tue Sep 11 11:33:47 2007 Subject: [Histonet] Lymph node fixative/enhance solutions Message-ID: Cindi: Polysciences,Inc manufactures Hartmanns Fixative which turns lymph nodes white. For more info see _www.polysciences.com_ (http://www.polysciences.com) Yes I am the Life Sciences product manager for the company Dana ************************************** See what's new at http://www.aol.com From gvdobbin <@t> ihis.org Tue Sep 11 11:46:10 2007 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Tue Sep 11 11:46:42 2007 Subject: [Histonet] Lymph node fixative/enhance solutions Message-ID: Hi Cindi, I recently read (with interest) about a product called Dissect Aid made by Decal Chemical Corp. I wanted to try it out in my gross room but unfortunately for us, it is not yet available in Canada-not a problem for you! Their toll free number is 800-428-5856 and you can find them on the web. Cheers! Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 >>> "Cindy Robinson" 9/11/2007 1:23:31 PM >>> Our pathologists would like us to start using a solution to help with the dissection and ID of lymph nodes at gross. I have done some searching thru the archives and found some recipes but would like to find a commercial source. We do use Pen-Fix but it doesn't dissolve the fat like the paths would like. Has anyone got a source or recipe for a fat dissolving solution that they would like to share? Thanks. Cindi Robinson, HT (ASCP) Dunes Medical Laboratories Dakota Dunes SD 57049 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ayant pour objet une personne ou un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer compl?tement de votre syst?me informatique. From jnocito <@t> satx.rr.com Tue Sep 11 11:49:00 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Sep 11 11:49:18 2007 Subject: [Histonet] Lymph node fixative/enhance solutions References: <6AF8653B3VO602000-01@EMF2> <46E67AB3.59AC.00AF.0@mercyhealth.com> Message-ID: <001101c7f493$a9ec1620$0202a8c0@yourxhtr8hvc4p> Cindi, Polyscientific has Carnoy's fluid but you really need to use it under the hood. Their number is 1-800-645-5825. They my an alternative. JTT ---- Original Message ----- From: "Cindy Robinson" To: Sent: Tuesday, September 11, 2007 11:23 AM Subject: [Histonet] Lymph node fixative/enhance solutions Our pathologists would like us to start using a solution to help with the dissection and ID of lymph nodes at gross. I have done some searching thru the archives and found some recipes but would like to find a commercial source. We do use Pen-Fix but it doesn't dissolve the fat like the paths would like. Has anyone got a source or recipe for a fat dissolving solution that they would like to share? Thanks. Cindi Robinson, HT (ASCP) Dunes Medical Laboratories Dakota Dunes SD 57049 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Sep 11 11:59:28 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Sep 11 11:59:41 2007 Subject: [Histonet] Lymph node fixative/enhance solutions In-Reply-To: Message-ID: <219484.59049.qm@web61212.mail.yahoo.com> Cindy: I used to prepare a solution that revealed the lymph nodes quite well: Ethanol 100%--- 2.0 litres + distilled water ---- 680 mL + 40% formalin ---- 320 mL + acetic acid -- 200 mL LN were revealed hardened and whitish Give it a try! Ren? J. >>> "Cindy Robinson" 9/11/2007 1:23:31 PM >>> Our pathologists would like us to start using a solution to help with the dissection and ID of lymph nodes at gross. I have done some searching thru the archives and found some recipes but would like to find a commercial source. We do use Pen-Fix but it doesn't dissolve the fat like the paths would like. Has anyone got a source or recipe for a fat dissolving solution that they would like to share? Thanks. Cindi Robinson, HT (ASCP) Dunes Medical Laboratories Dakota Dunes SD 57049 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ayant pour objet une personne ou un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer compl?tement de votre syst?me informatique. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better Globetrotter. Get better travel answers from someone who knows. Yahoo! Answers - Check it out. From jnocito <@t> satx.rr.com Tue Sep 11 12:02:57 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Sep 11 12:03:21 2007 Subject: [Histonet] Worlds Largest Paraffin Dispenser References: <68gdri$1fpqeb9@hrndva-mx-12.mgw.rr.com> Message-ID: <006201c7f495$9c293840$0202a8c0@yourxhtr8hvc4p> I know TBS and ThermoFisher have 5 gallon pots. How big do you need? I'm getting visions of hot paraffin in a hot tub. Any vendors want to try that for Denver? I mean, it will be the end of October. Joe ----- Original Message ----- From: "Douglas D Deltour" To: Sent: Tuesday, September 11, 2007 11:49 AM Subject: [Histonet] Worlds Largest Paraffin Dispenser >I am looking for a paraffin dispenser that holds a LARGE amount of >paraffin. > My requests for a hot tub that I can fill with paraffin have been denied > for > some reason. Anyone know of a manufacturer of these large dispensers? > > > > Douglas D. Deltour HT(ASCP) > > Histology Manager > > Professional Pathology Services, PC > > One Science Court > > Suite 200 > > Columbia, SC 29203 > > Office (803)252-1913 > > Fax (803)254-3262 > > Doug@ppspath.com > > ***************************************************** > > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of the individual or entity to > which it is addressed and may contain information that is privileged, > confidential and exempt from disclosure under applicable law. If the > reader > of this message is not the intended recipient, you are hereby notified > that > any dissemination, distribution, or copying of this communication is > strictly prohibited by law. If you have received this communication in > error, please notify me immediately. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Tue Sep 11 12:06:27 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Sep 11 12:06:40 2007 Subject: [Histonet] Lymph node fixative/enhance solutions In-Reply-To: <46E67AB3.59AC.00AF.0@mercyhealth.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C714@IS-E2K3.grhs.net> We used straight acetone and the fat turns translucent and the nodes turn white within 10 minutes. Easy to get, cheap and no mixing or purchasing reagents. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy Robinson Sent: Tuesday, September 11, 2007 11:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Lymph node fixative/enhance solutions Our pathologists would like us to start using a solution to help with the dissection and ID of lymph nodes at gross. I have done some searching thru the archives and found some recipes but would like to find a commercial source. We do use Pen-Fix but it doesn't dissolve the fat like the paths would like. Has anyone got a source or recipe for a fat dissolving solution that they would like to share? Thanks. Cindi Robinson, HT (ASCP) Dunes Medical Laboratories Dakota Dunes SD 57049 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ROrr <@t> enh.org Tue Sep 11 12:31:49 2007 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Tue Sep 11 12:32:03 2007 Subject: [Histonet] Dissect Aid Message-ID: We use Dissect Aid in our gross room for the application you've referenced. Thanks for sharing the Home Brew though! We get it from Decal Corp and it works very well It's got Acetic Acid and Formalin (40% I believe) And prolly some other stabilizer. Becky Orr CLA,HT(ASCP)QIHC Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 From vazquezr <@t> ohsu.edu Tue Sep 11 12:38:36 2007 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Tue Sep 11 12:39:11 2007 Subject: [Histonet] Worlds Largest Paraffin Dispenser Message-ID: Hacker carries them, around $1000.00. Robyn >>> "Douglas D Deltour" 9/11/2007 9:49 AM >>> I am looking for a paraffin dispenser that holds a LARGE amount of paraffin. My requests for a hot tub that I can fill with paraffin have been denied for some reason. Anyone know of a manufacturer of these large dispensers? Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From agoldstein <@t> KaiserAssociates.com Tue Sep 11 12:45:00 2007 From: agoldstein <@t> KaiserAssociates.com (Adam Goldstein) Date: Tue Sep 11 12:43:10 2007 Subject: [Histonet] Histology Consumables Product Survey ($50 Honorarium opportunity) Message-ID: Dear Histonetters, Our firm, Kaiser Associates, is conducting research on the products and processes for the histologic technique of primary (H&E) staining and would like your input. By participating in this survey, you will be influencing potential upcoming changes to the market for primary (H&E) staining products. The completion of this survey should only take 20-30 minutes, and in return for your participation you will receive an honorarium of US $50 in the form of an American Express gift card. If you would like to participate in this survey please reply to AGoldstein@KaiserAssociates.com with: 1. Your job title; 2. Your department; and 3. Your company/hospital name and location Upon receipt of the above information, we will send you the web link to the survey. Please note that we are primarily interested in respondents from the US or Europe. Thanks! Sincerely, Kaiser Associates, Inc. Research Team About Kaiser Associates, Inc: Kaiser Associates is a research-based international consulting firm dedicated to helping leading global corporations develop effective strategies to drive continued operating performance. For more information please go to www.KaiserAssociates.com. From RSRICHMOND <@t> aol.com Tue Sep 11 12:52:33 2007 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Tue Sep 11 12:52:48 2007 Subject: [Histonet] Re: Lymph node fixative/enhance solutions Message-ID: Cindi Robinson, HT (ASCP) in South Dakota asks: >>Our pathologists would like us to start using a solution to help with the dissection and identification of lymph nodes at gross. - We do use Pen-Fix but it doesn't dissolve the fat like the paths would like.<< And Dana Dittus at Polysciences notes: >>Polysciences,Inc manufactures Hartmanns Fixative which turns lymph nodes white. For more info see www.polysciences.com << It's easy and cheap to prepare Davidson's fixative (same as Hartmann's fixative) - mix 3 parts water, 3 parts reagent alcohol, 2 parts strong (37%) formalin, not the buffered kind, and 1 part glacial acetic acid. Color it with eosin if you want to. When Dr. William Hartmann (later the chair of the American Board of Pathology) was at Johns Hopkins in the early 1960's, Dr. Victor McCusick (the medical geneticist, later chair of medicine at Hopkins) asked him to introduce the fixative for Barr bodies (sex chromatin bodies) that Moore and Barr had specified in their original articles on Barr bodies around 1954. Moore and Barr specified "modified Davidson's fixative", which Bill Hartmann introduced. It came into rather widespread use at Hopkins, and Hartmann's name stuck to it. I think the name should not be used, because of possible disastrous confusion with Hartmann's solution, lactated Ringer's solution, introduced into pediatric practice in the 1930's by Dr. Alexis Hartmann Sr. at Washington University in St.Louis. Dr. Alexis Hartmann was one of my clinical teachers, right before he retired around 1964 (sorry about all this name dropping). John Kiernan and I discussed Davidson's fixative in this forum several years ago. He noted that it is not really a rational formula. He hoped to have the time to research the fixative in Davidson's papers, since it seems never to have been published. Getting back to the actual subject, I've used Dissect Aid (Decal Corporation) with good results - got a bottle of it on the shelf right now, in fact. In my brief experience with Penn-Fix, it did not disclose lymph nodes well. Let me support your pathologists very strongly in this endeavor. Just one positive lymph node upstages a colon cancer and makes chemotherapy mandatory. And in colon cancer, those postive lymph nodes can be the size of pinheads. Bob Richmond Samurai Pathologist Knoxville TN From jnocito <@t> satx.rr.com Tue Sep 11 12:02:57 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Sep 11 14:29:06 2007 Subject: [Histonet] Worlds Largest Paraffin Dispenser References: <68gdri$1fpqeb9@hrndva-mx-12.mgw.rr.com> Message-ID: <000001c7f4a9$fcd58310$0202a8c0@yourxhtr8hvc4p> I know TBS and ThermoFisher have 5 gallon pots. How big do you need? I'm getting visions of hot paraffin in a hot tub. Any vendors want to try that for Denver? I mean, it will be the end of October. Joe ----- Original Message ----- From: "Douglas D Deltour" To: Sent: Tuesday, September 11, 2007 11:49 AM Subject: [Histonet] Worlds Largest Paraffin Dispenser >I am looking for a paraffin dispenser that holds a LARGE amount of >paraffin. > My requests for a hot tub that I can fill with paraffin have been denied > for > some reason. Anyone know of a manufacturer of these large dispensers? > > > > Douglas D. Deltour HT(ASCP) > > Histology Manager > > Professional Pathology Services, PC > > One Science Court > > Suite 200 > > Columbia, SC 29203 > > Office (803)252-1913 > > Fax (803)254-3262 > > Doug@ppspath.com > > ***************************************************** > > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of the individual or entity to > which it is addressed and may contain information that is privileged, > confidential and exempt from disclosure under applicable law. If the > reader > of this message is not the intended recipient, you are hereby notified > that > any dissemination, distribution, or copying of this communication is > strictly prohibited by law. If you have received this communication in > error, please notify me immediately. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From m5johnso <@t> meded.ucsd.edu Tue Sep 11 14:58:31 2007 From: m5johnso <@t> meded.ucsd.edu (Mindy Johnson) Date: Tue Sep 11 14:58:48 2007 Subject: [Histonet] Looking for a specific type of slide tray..... Message-ID: <007301c7f4ae$212c80c0$63858240$@ucsd.edu> Hi All- We are looking for some trays that hold slides. We currently have about 3 and I need more. They look old school and I have no clue the technical term for them. But, they hold about 50 slides (5 rows hold about 10 each), are flat on one side and the other is divided into the 5 rows, the material is made of that stuff that resembles a wood like fiberboard. They are extremely handy for our lab and good for after cutting I put in the incubator to make sure they are totally dry before staining. Anyone have any idea where I can find something similar to this? Thank you so much for your help! Mindy From b-frederick <@t> northwestern.edu Tue Sep 11 15:00:52 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Tue Sep 11 15:01:16 2007 Subject: [Histonet] Worlds Largest Paraffin Dispenser In-Reply-To: <000001c7f4a9$fcd58310$0202a8c0@yourxhtr8hvc4p> Message-ID: <001a01c7f4ae$77f76fa0$d00f7ca5@lurie.northwestern.edu> One BIG candle for someones birthday!!!!!!!!! Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Tuesday, September 11, 2007 12:03 PM To: Douglas D Deltour; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Worlds Largest Paraffin Dispenser I know TBS and ThermoFisher have 5 gallon pots. How big do you need? I'm getting visions of hot paraffin in a hot tub. Any vendors want to try that for Denver? I mean, it will be the end of October. Joe ----- Original Message ----- From: "Douglas D Deltour" To: Sent: Tuesday, September 11, 2007 11:49 AM Subject: [Histonet] Worlds Largest Paraffin Dispenser >I am looking for a paraffin dispenser that holds a LARGE amount of >paraffin. > My requests for a hot tub that I can fill with paraffin have been denied > for > some reason. Anyone know of a manufacturer of these large dispensers? > > > > Douglas D. Deltour HT(ASCP) > > Histology Manager > > Professional Pathology Services, PC > > One Science Court > > Suite 200 > > Columbia, SC 29203 > > Office (803)252-1913 > > Fax (803)254-3262 > > Doug@ppspath.com > > ***************************************************** > > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of the individual or entity to > which it is addressed and may contain information that is privileged, > confidential and exempt from disclosure under applicable law. If the > reader > of this message is not the intended recipient, you are hereby notified > that > any dissemination, distribution, or copying of this communication is > strictly prohibited by law. If you have received this communication in > error, please notify me immediately. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Benjamin.Kriederman <@t> providence.org Tue Sep 11 15:05:43 2007 From: Benjamin.Kriederman <@t> providence.org (Kriederman, Ben) Date: Tue Sep 11 15:08:16 2007 Subject: [Histonet] Opinion on Microm automated microtomes? Message-ID: Does anyone have an opinion on the microm hm355s automated microtome? When it comes to cutting hard tissues we are getting thick and thin, any suggestions? Thick and Thin in Alaska DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From slappycraw <@t> yahoo.com Tue Sep 11 15:10:54 2007 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Tue Sep 11 15:11:08 2007 Subject: [Histonet] Worlds Largest Paraffin Dispenser Message-ID: <308814.42020.qm@web53605.mail.re2.yahoo.com> I've heard of people getting into their work before but embedding yourself? --------------------------------- Be a better Heartthrob. Get better relationship answers from someone who knows. Yahoo! Answers - Check it out. From HornHV <@t> archildrens.org Tue Sep 11 15:34:29 2007 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Tue Sep 11 15:34:51 2007 Subject: [Histonet] Looking for a specific type of slide tray..... In-Reply-To: <007301c7f4ae$212c80c0$63858240$@ucsd.edu> References: <007301c7f4ae$212c80c0$63858240$@ucsd.edu> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB7335@EMAIL.archildrens.org> I know I ordered some of these trays before and the company was called brain research labs? But I looked on the internet and cannot find anything. The flats were trimmed in wood with a white background. Each row held 10 slides and there are 5 rows on the board. Maybe someone else can help you. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mindy Johnson Sent: Tuesday, September 11, 2007 2:59 PM To: 'HistoNet' Subject: [Histonet] Looking for a specific type of slide tray..... Hi All- We are looking for some trays that hold slides. We currently have about 3 and I need more. They look old school and I have no clue the technical term for them. But, they hold about 50 slides (5 rows hold about 10 each), are flat on one side and the other is divided into the 5 rows, the material is made of that stuff that resembles a wood like fiberboard. They are extremely handy for our lab and good for after cutting I put in the incubator to make sure they are totally dry before staining. Anyone have any idea where I can find something similar to this? Thank you so much for your help! Mindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From judi.ford <@t> roche.com Tue Sep 11 15:38:56 2007 From: judi.ford <@t> roche.com (Ford, Judi) Date: Tue Sep 11 15:39:18 2007 Subject: [Histonet] Opinion on Microm automated microtomes? In-Reply-To: References: Message-ID: I've been working with that model for quite sometime now and once in awhile I get thick and thin sections. Usually it happens when something is loose or I'm cutting in the middle of the blade. I tend to cut on either end of the knife blade, avoiding the middle of the blade holder when I can. Check the knife angle (9 or 10). Also, try checking the block to see that it is properly seated in the chuck and tight. Hope this helps. Good luck. Judi Ford Palo Alto, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kriederman, Ben Sent: Tuesday, September 11, 2007 1:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Opinion on Microm automated microtomes? Does anyone have an opinion on the microm hm355s automated microtome? When it comes to cutting hard tissues we are getting thick and thin, any suggestions? Thick and Thin in Alaska DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From m5johnso <@t> meded.ucsd.edu Tue Sep 11 16:15:42 2007 From: m5johnso <@t> meded.ucsd.edu (Mindy Johnson) Date: Tue Sep 11 16:17:35 2007 Subject: [Histonet] Looking for a specific type of slide tray..... In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB7335@EMAIL.archildrens.org> References: <007301c7f4ae$212c80c0$63858240$@ucsd.edu> <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB7335@EMAIL.archildrens.org> Message-ID: <007e01c7f4b8$e9c5c280$bd514780$@ucsd.edu> Yes it is Brain Research Labs! They are exactly what I was looking for! THANK YOU SOOOO MUCH!! ?Mindy A Johnson SRA II UCSD - School of Medicine Medical Teaching Labs -----Original Message----- From: Horn, Hazel V [mailto:HornHV@archildrens.org] Sent: Tuesday, September 11, 2007 1:34 PM To: Mindy Johnson; HistoNet Subject: RE: [Histonet] Looking for a specific type of slide tray..... I know I ordered some of these trays before and the company was called brain research labs? But I looked on the internet and cannot find anything. The flats were trimmed in wood with a white background. Each row held 10 slides and there are 5 rows on the board. Maybe someone else can help you. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mindy Johnson Sent: Tuesday, September 11, 2007 2:59 PM To: 'HistoNet' Subject: [Histonet] Looking for a specific type of slide tray..... Hi All- We are looking for some trays that hold slides. We currently have about 3 and I need more. They look old school and I have no clue the technical term for them. But, they hold about 50 slides (5 rows hold about 10 each), are flat on one side and the other is divided into the 5 rows, the material is made of that stuff that resembles a wood like fiberboard. They are extremely handy for our lab and good for after cutting I put in the incubator to make sure they are totally dry before staining. Anyone have any idea where I can find something similar to this? Thank you so much for your help! Mindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------------- -- The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================ == From raj <@t> bluemarble.net Tue Sep 11 18:32:37 2007 From: raj <@t> bluemarble.net (Rebecca Johnson) Date: Tue Sep 11 17:21:50 2007 Subject: [Histonet] Special Stainers Message-ID: <011a01c7f4cc$09fefcc0$7b48f9d8@CHURCH> What special stainers is everyone using? I now have a Ventana Nexus. Bought in 1999. I know this is old, but it has been maintained well. We have had problems with it several times over the years. Mostly with sliver stains or uneven staining and no staining. I would like to know what others are using and your experiences are. Thanks for all you help. raj From raj <@t> bluemarble.net Tue Sep 11 18:39:37 2007 From: raj <@t> bluemarble.net (Rebecca Johnson) Date: Tue Sep 11 17:28:42 2007 Subject: [Histonet] Special Stainers Message-ID: <012801c7f4cd$045aa1b0$7b48f9d8@CHURCH> Need advice on special stainers everyone is using. We now have Ventana Nexus special stainer.Bought in 1999. I know this is old but it has been maintained well. We have always had a service contract with Ventana. Have had problems over the years with the silver stains, no staining and uneven staining. Now they want to tell me that this is normal with any company. For the amount of money these stainers cost that is just little more than I am willing to believe. Thanks for all you help. raj From raj <@t> bluemarble.net Tue Sep 11 19:00:33 2007 From: raj <@t> bluemarble.net (Rebecca Johnson) Date: Tue Sep 11 17:49:37 2007 Subject: [Histonet] Histnet Special Stainers Message-ID: <017101c7f4cf$f0967bb0$7b48f9d8@CHURCH> Need advice on what special stainers everyone is using. The good and bad. We are now using Ventana Nexus special stainer. Bought in 1999. Not real happy with what I am hearing about all stainers. I was told today by a manager that all stainers have problems a couple times a year. We have had uneven staining, no staining and problems with the silver stain. For what they cost and the cost of the maintenance contracts, this is hard for me to believe. What is your experience with the stainers. Thanks raj From Ribonukle <@t> yahoo.com.ar Tue Sep 11 18:33:58 2007 From: Ribonukle <@t> yahoo.com.ar (ribonukle) Date: Tue Sep 11 18:33:56 2007 Subject: [Histonet] Opinion on Microm automated microtomes? References: Message-ID: <01e801c7f4cc$3b8adb10$590215ac@Pentium4> I have a similar model and also has that problem, thick and thin with all very good adjusted.they have already become unstuck and broken several things This microm Microtome have low quality Hope this helps. Ribo ----- Original Message ----- From: "Ford, Judi" To: "Kriederman, Ben" ; Sent: Tuesday, September 11, 2007 5:38 PM Subject: RE: [Histonet] Opinion on Microm automated microtomes? I've been working with that model for quite sometime now and once in awhile I get thick and thin sections. Usually it happens when something is loose or I'm cutting in the middle of the blade. I tend to cut on either end of the knife blade, avoiding the middle of the blade holder when I can. Check the knife angle (9 or 10). Also, try checking the block to see that it is properly seated in the chuck and tight. Hope this helps. Good luck. Judi Ford Palo Alto, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kriederman, Ben Sent: Tuesday, September 11, 2007 1:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Opinion on Microm automated microtomes? Does anyone have an opinion on the microm hm355s automated microtome? When it comes to cutting hard tissues we are getting thick and thin, any suggestions? Thick and Thin in Alaska DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Tony_Reilly <@t> health.qld.gov.au Tue Sep 11 19:01:05 2007 From: Tony_Reilly <@t> health.qld.gov.au (Anthony Reilly) Date: Tue Sep 11 19:01:35 2007 Subject: [Histonet] Opinion on Microm automated microtomes? Message-ID: I agree. I have 2 old Microms purchased in the early nineties which were very good on purchase and are still going strong however we have another that was purchased about 2 years ago and there is a marked decrease in the sectioning ability. Even the physical appearance of the instument is notably inferior. Some blocks will cut thick and thin for no apparent reason. They do not even have to be hard tissue. regards Tony Reilly Chief Scientist Anatomical Pathology QHPS-Prince Charles Hospital Rode Rd Chermside Q 4032 Australia Ph: 07 3139 4543 Fax: 07 3193 4546 tony_reilly@health.qld.gov.au >>> "ribonukle" 09/12/07 9:33 am >>> I have a similar model and also has that problem, thick and thin with all very good adjusted.they have already become unstuck and broken several things This microm Microtome have low quality Hope this helps. Ribo ----- Original Message ----- From: "Ford, Judi" To: "Kriederman, Ben" ; Sent: Tuesday, September 11, 2007 5:38 PM Subject: RE: [Histonet] Opinion on Microm automated microtomes? I've been working with that model for quite sometime now and once in awhile I get thick and thin sections. Usually it happens when something is loose or I'm cutting in the middle of the blade. I tend to cut on either end of the knife blade, avoiding the middle of the blade holder when I can. Check the knife angle (9 or 10). Also, try checking the block to see that it is properly seated in the chuck and tight. Hope this helps. Good luck. Judi Ford Palo Alto, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kriederman, Ben Sent: Tuesday, September 11, 2007 1:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Opinion on Microm automated microtomes? Does anyone have an opinion on the microm hm355s automated microtome? When it comes to cutting hard tissues we are getting thick and thin, any suggestions? Thick and Thin in Alaska DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/ received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. **************************************************************** From cmalc <@t> unimelb.edu.au Wed Sep 12 00:28:32 2007 From: cmalc <@t> unimelb.edu.au (Cathy Malcontenti-Wilson) Date: Wed Sep 12 00:29:46 2007 Subject: [Histonet] co-localisation within a cell using DAB and Vulcan Fast Red Message-ID: <6.2.1.2.2.20070912152507.0421f230@mail.staff.unimelb.edu.au> Dear Histonetters, Does anyone have experience with co-localisation of the chromogens DAB and Vulcan Fast Red in an immunohistochemical stain? I have just done a double stain and I would like to know what I should I expect if the two antigens I have stained are co-localised in the one cell? Would it to look red or orange or what?? Much appreciated, Thanks Cathy Malcontenti-Wilson From cbass <@t> wfubmc.edu Wed Sep 12 09:58:54 2007 From: cbass <@t> wfubmc.edu (Caroline Bass) Date: Wed Sep 12 10:01:45 2007 Subject: [Histonet] AO 860 sliding microtome Message-ID: Hi guys, So I've inherited an American Optical 860 sliding microtome. I consider this quite a find since it is my all time favorite for slicing fixed brains. However, it has been packed away for quite a while and seems to be a bit gummed up and is in parts. Does anyone have suggestions on how to get this back into working order. I don't suppose there is a manual floating around? Any general suggestions for degreasing to remove the stick points. How about a location for spare parts, particularly dry ice stages and maybe a disposable blade holder? Any and all suggestions are appreciated. Thanks, Caroline Bass From FUNKM <@t> mercyhealth.com Wed Sep 12 10:29:57 2007 From: FUNKM <@t> mercyhealth.com (Marcia Funk) Date: Wed Sep 12 10:30:24 2007 Subject: [Histonet] QC labeling Message-ID: <46E7BFA5.9B87.00AC.0@mercyhealth.com> Histo Friends, I have a few questions I would like to see how labs are following through with labeling errors? Case number placed on slide during sectioning. Slide is then mislabeled handed off to pathologist. Pathologist discovers the error. Slide is labeled incorrectly. Wrong label placed on slide during labeling. Pathologist discovers mislabeld slide Errors are place on QC log at the end of day. These are errors that are caught in the department by the tech or pathologist. Do most labs keep a log with errors by tech and how many errors impact there eval ? I would appreciate your imput. Mrf From rjbuesa <@t> yahoo.com Wed Sep 12 10:31:03 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 12 10:32:17 2007 Subject: [Histonet] AO 860 sliding microtome In-Reply-To: Message-ID: <753832.79448.qm@web61225.mail.yahoo.com> Caroline: I learned to cut in one of those (in 1952) and I still consider this model of horizontal microtome the finest ever built, specially with its clever vertical automatic feeding mechanism. IF you have mechanical dextereity, it is not difficult to put it back in working conditoins. As you can see, almost every part is screwed and even the feeding mechanisms is like a single unit. You will have to unscrew it all and place each component in a bucket with mineral spirits or any other grease solvent. Even kerosene will work. Leave the parts in the degreaser for at least 3 days, you will see how the old grease will dissolve and color the degreaser. Air dry it (presurized air). Repeat the cleaning (degreasing) if it still looks greasy. After you see each part is clean and the nickel chorme is gleaming and sparkling, grease it up (with the same type of microtome grease you would use for any modern microtome; I always used the Carl Jung light oil No. 405; Leica has also a very fine light oil,called "Schlitten-bahnenol Nr. 601". The feeding trigger has to be lubricated with light oil, because its return to vertical is caused by gravity, and cannot be stuck in any way. The screw for the feeding mechanism has to receive solid grease (Jelly lubrican), I always used the Lipshaw No.294 This type of maintenance I used to do, at least annually. Enjoy your cleaning! Ren? J. Caroline Bass wrote: Hi guys, So I've inherited an American Optical 860 sliding microtome. I consider this quite a find since it is my all time favorite for slicing fixed brains. However, it has been packed away for quite a while and seems to be a bit gummed up and is in parts. Does anyone have suggestions on how to get this back into working order. I don't suppose there is a manual floating around? Any general suggestions for degreasing to remove the stick points. How about a location for spare parts, particularly dry ice stages and maybe a disposable blade holder? Any and all suggestions are appreciated. Thanks, Caroline Bass _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better Globetrotter. Get better travel answers from someone who knows. Yahoo! Answers - Check it out. From rjbuesa <@t> yahoo.com Wed Sep 12 10:39:55 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 12 10:51:50 2007 Subject: [Histonet] QC labeling In-Reply-To: <46E7BFA5.9B87.00AC.0@mercyhealth.com> Message-ID: <531579.50543.qm@web61222.mail.yahoo.com> Marcia: Short answer, yes! I used to keep a file for each HT, and in it everything was written down: the good, the bad, the uggly; trainings, commendations, attendance, you name it. It was updated everytime anything worth mentioning took place, from commendations from the pathologists for good sections, staining, whatever; as well as any mistakes, not only dealing with mislabelling, but with poor quality of sections, floaters, as I wrote, every thing. Come the evaluation day all those things, good and bad, all documented AT THE MOMENT THEY HAPPENED, not after the fact, we weighed and most certainly affected the evaluation, either positively or negatively. Once that evaluation was completed, signed, agreed or disagreed, that annual file was incorporated to the personal file, and a NEW file was opened for the next evaluation period. Since all issues were addressed immediately they happened or were found, I never had a single case of a HT saying: "I did not know about that", they knew and received retraining if necessary. Ren? J. Marcia Funk wrote: Histo Friends, I have a few questions I would like to see how labs are following through with labeling errors? Case number placed on slide during sectioning. Slide is then mislabeled handed off to pathologist. Pathologist discovers the error. Slide is labeled incorrectly. Wrong label placed on slide during labeling. Pathologist discovers mislabeld slide Errors are place on QC log at the end of day. These are errors that are caught in the department by the tech or pathologist. Do most labs keep a log with errors by tech and how many errors impact there eval ? I would appreciate your imput. Mrf _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Building a website is a piece of cake. Yahoo! Small Business gives you all the tools to get online. From mari.ann.mailhiot <@t> leica-microsystems.com Wed Sep 12 11:45:47 2007 From: mari.ann.mailhiot <@t> leica-microsystems.com (mari.ann.mailhiot@leica-microsystems.com) Date: Wed Sep 12 11:46:07 2007 Subject: [Histonet] AO 860 sliding microtome In-Reply-To: Message-ID: Hi Caroline Please contact me here at Leica about the AO860 sliding microtome. Rene has offered an excellent clean application for this unit. Thanks Renee. Best Regards Mari Ann Mailhiot BA HT ASCP Application Specialist Leica Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From Jackie.O'Connor <@t> abbott.com Wed Sep 12 12:16:27 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed Sep 12 12:17:16 2007 Subject: [Histonet] Histology apprentice job description In-Reply-To: Message-ID: Does anyone have a sample job description for a histology apprentice? I'll give you a dollar for it. From lteves <@t> uhnres.utoronto.ca Wed Sep 12 12:24:18 2007 From: lteves <@t> uhnres.utoronto.ca (lteves) Date: Wed Sep 12 12:24:32 2007 Subject: [Histonet] solvent containers Message-ID: <46E820C2.2F85CC31@uhnres.utoronto.ca> Hi there in histo land, We have an old Histomatic Tissue Processor Model 166 MP and looking for replacement solvent containers. If you have any and willing to part with, please contact me. Or if you know where I may get my hands on some. lteves@uhnres.utoronto.ca Thanks in advance L. From gu.lang <@t> gmx.at Wed Sep 12 14:17:48 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Sep 12 14:17:59 2007 Subject: AW: [Histonet] Opinion on Microm automated microtomes? In-Reply-To: Message-ID: <000001c7f571$9c1b68a0$6412a8c0@dielangs.at> Once we had such a problem it was due to the knife-holder. One screw was a little bit loose. So I screwed it firm while the knife stick in the holder to keep the right distance (attention!). Then cutting was well again. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Kriederman, Ben Gesendet: Dienstag, 11. September 2007 22:06 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Opinion on Microm automated microtomes? Does anyone have an opinion on the microm hm355s automated microtome? When it comes to cutting hard tissues we are getting thick and thin, any suggestions? Thick and Thin in Alaska DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histomike <@t> charter.net Wed Sep 12 14:51:34 2007 From: histomike <@t> charter.net (Mike Schlicht) Date: Wed Sep 12 14:51:37 2007 Subject: [Histonet] Special Stainers References: <20070912164649.HUF2247.aarpub08.charter.net@swlx162.swmed.edu> Message-ID: <001a01c7f576$53721540$6401a8c0@yourvp7x3s9ctm> We are currently using the DAKO Artisan for silver stains, and have some problems with uneven staining every now and then, usually when the dispensers are close to being empty, so we stop using the kit when there are 5 tests remaining to help reduce some of the problems. We had a demo of the Nexus stainer, and found that the result were about the same. Mike San Luis Obispo, CA > Message: 15 > Date: Tue, 11 Sep 2007 18:32:37 -0500 > From: "Rebecca Johnson" > Subject: [Histonet] Special Stainers > To: "histonet" > Message-ID: <011a01c7f4cc$09fefcc0$7b48f9d8@CHURCH> > Content-Type: text/plain; charset="iso-8859-1" > > What special stainers is everyone using? I now have a Ventana Nexus. > Bought in 1999. I know this is old, but it has been maintained well. We > have had problems with it several times over the years. Mostly with sliver > stains or uneven staining and no staining. I would like to know what > others are using and your experiences are. Thanks for all you help. > raj > From sbreeden <@t> nmda.nmsu.edu Wed Sep 12 16:00:46 2007 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Sep 12 16:01:04 2007 Subject: [Histonet] Histonet at NSH?? Message-ID: <4D14F0FC9316DD41972D5F03C070908B8F4689@nmdamailsvr.nmda.ad.nmsu.edu> Will there be a Histonet Gathering at NSH in Denver this year? Or would someone like to plan one? I have recently resigned from my position as The Pearl (Perle?) Mesta of That alone ought to tell you that I'm at least as old as Rene (just joshin', Rene...). Maybe a gathering is not a good idea if I keep insulting perfect strangers, huh? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From Joanne.Malinowski <@t> crl.com Wed Sep 12 16:13:16 2007 From: Joanne.Malinowski <@t> crl.com (Malinowski, Joanne O,) Date: Wed Sep 12 16:13:39 2007 Subject: [Histonet] Spur Resin In-Reply-To: <67tift$8cnb6@mail01.us.crl.com> Message-ID: <3E94391DD24ECE418DCBA5EF74BA29940B36B5@shr-exch1.na01.crl.com> Hello Plastic People, Could anyone share their recipe for using spur resin to produce plastic sections? Thank you all in advance for offering any help. Best regards, Joanne Joanne Malinowski,HT ASCP Plastics Lab Manager, Medical Devices Division Charles River Laboratories Pathology Associates 15 Worman's Mill Court, Suite I Frederick, Maryland 21701 Phone 301-624-2034 Fax 301-663-8994 Email: joanne.malinowski@us.crl.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, September 12, 2007 12:46 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 46, Issue 18 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Worlds Largest Paraffin Dispenser (Joe Nocito) 2. RE: Lymph node fixative/enhance solutions (Mike Pence) 3. Dissect Aid (Orr, Rebecca) 4. Re: Worlds Largest Paraffin Dispenser (Robyn Vazquez) 5. Histology Consumables Product Survey ($50 Honorarium opportunity) (Adam Goldstein) 6. Re: Lymph node fixative/enhance solutions (Robert Richmond) 7. Re: Worlds Largest Paraffin Dispenser (Joe Nocito) 8. Looking for a specific type of slide tray..... (Mindy Johnson) 9. RE: Worlds Largest Paraffin Dispenser (Bernice Frederick) 10. Opinion on Microm automated microtomes? (Kriederman, Ben) 11. Re: Worlds Largest Paraffin Dispenser (Larry Woody) 12. RE: Looking for a specific type of slide tray..... (Horn, Hazel V) 13. RE: Opinion on Microm automated microtomes? (Ford, Judi) 14. RE: Looking for a specific type of slide tray..... (Mindy Johnson) 15. Special Stainers (Rebecca Johnson) 16. Special Stainers (Rebecca Johnson) 17. Histnet Special Stainers (Rebecca Johnson) 18. Re: Opinion on Microm automated microtomes? (ribonukle) 19. Re: Opinion on Microm automated microtomes? (Anthony Reilly) 20. co-localisation within a cell using DAB and Vulcan Fast Red (Cathy Malcontenti-Wilson) 21. AO 860 sliding microtome (Caroline Bass) 22. QC labeling (Marcia Funk) 23. Re: AO 860 sliding microtome (Rene J Buesa) 24. Re: QC labeling (Rene J Buesa) 25. Re: AO 860 sliding microtome (mari.ann.mailhiot@leica-microsystems.com) ---------------------------------------------------------------------- Message: 1 Date: Tue, 11 Sep 2007 12:02:57 -0500 From: "Joe Nocito" Subject: Re: [Histonet] Worlds Largest Paraffin Dispenser To: "Douglas D Deltour" , Message-ID: <006201c7f495$9c293840$0202a8c0@yourxhtr8hvc4p> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original I know TBS and ThermoFisher have 5 gallon pots. How big do you need? I'm getting visions of hot paraffin in a hot tub. Any vendors want to try that for Denver? I mean, it will be the end of October. Joe ----- Original Message ----- From: "Douglas D Deltour" To: Sent: Tuesday, September 11, 2007 11:49 AM Subject: [Histonet] Worlds Largest Paraffin Dispenser >I am looking for a paraffin dispenser that holds a LARGE amount of >paraffin. > My requests for a hot tub that I can fill with paraffin have been denied > for > some reason. Anyone know of a manufacturer of these large dispensers? > > > > Douglas D. Deltour HT(ASCP) > > Histology Manager > > Professional Pathology Services, PC > > One Science Court > > Suite 200 > > Columbia, SC 29203 > > Office (803)252-1913 > > Fax (803)254-3262 > > Doug@ppspath.com > > ***************************************************** > > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of the individual or entity to > which it is addressed and may contain information that is privileged, > confidential and exempt from disclosure under applicable law. If the > reader > of this message is not the intended recipient, you are hereby notified > that > any dissemination, distribution, or copying of this communication is > strictly prohibited by law. If you have received this communication in > error, please notify me immediately. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Tue, 11 Sep 2007 12:06:27 -0500 From: "Mike Pence" Subject: RE: [Histonet] Lymph node fixative/enhance solutions To: "Cindy Robinson" , Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C714@IS-E2K3.grhs.net> Content-Type: text/plain; charset="us-ascii" We used straight acetone and the fat turns translucent and the nodes turn white within 10 minutes. Easy to get, cheap and no mixing or purchasing reagents. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy Robinson Sent: Tuesday, September 11, 2007 11:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Lymph node fixative/enhance solutions Our pathologists would like us to start using a solution to help with the dissection and ID of lymph nodes at gross. I have done some searching thru the archives and found some recipes but would like to find a commercial source. We do use Pen-Fix but it doesn't dissolve the fat like the paths would like. Has anyone got a source or recipe for a fat dissolving solution that they would like to share? Thanks. Cindi Robinson, HT (ASCP) Dunes Medical Laboratories Dakota Dunes SD 57049 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Tue, 11 Sep 2007 12:31:49 -0500 From: "Orr, Rebecca" Subject: [Histonet] Dissect Aid To: Message-ID: Content-Type: text/plain; charset="us-ascii" We use Dissect Aid in our gross room for the application you've referenced. Thanks for sharing the Home Brew though! We get it from Decal Corp and it works very well It's got Acetic Acid and Formalin (40% I believe) And prolly some other stabilizer. Becky Orr CLA,HT(ASCP)QIHC Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 ------------------------------ Message: 4 Date: Tue, 11 Sep 2007 10:38:36 -0700 From: "Robyn Vazquez" Subject: Re: [Histonet] Worlds Largest Paraffin Dispenser To: histonet@lists.utsouthwestern.edu, doug@ppspath.com Message-ID: Content-Type: text/plain; charset=us-ascii Hacker carries them, around $1000.00. Robyn >>> "Douglas D Deltour" 9/11/2007 9:49 AM >>> I am looking for a paraffin dispenser that holds a LARGE amount of paraffin. My requests for a hot tub that I can fill with paraffin have been denied for some reason. Anyone know of a manufacturer of these large dispensers? Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Tue, 11 Sep 2007 13:45:00 -0400 From: "Adam Goldstein" Subject: [Histonet] Histology Consumables Product Survey ($50 Honorarium opportunity) To: Message-ID: Content-Type: text/plain; charset="us-ascii" Dear Histonetters, Our firm, Kaiser Associates, is conducting research on the products and processes for the histologic technique of primary (H&E) staining and would like your input. By participating in this survey, you will be influencing potential upcoming changes to the market for primary (H&E) staining products. The completion of this survey should only take 20-30 minutes, and in return for your participation you will receive an honorarium of US $50 in the form of an American Express gift card. If you would like to participate in this survey please reply to AGoldstein@KaiserAssociates.com with: 1. Your job title; 2. Your department; and 3. Your company/hospital name and location Upon receipt of the above information, we will send you the web link to the survey. Please note that we are primarily interested in respondents from the US or Europe. Thanks! Sincerely, Kaiser Associates, Inc. Research Team About Kaiser Associates, Inc: Kaiser Associates is a research-based international consulting firm dedicated to helping leading global corporations develop effective strategies to drive continued operating performance. For more information please go to www.KaiserAssociates.com. ------------------------------ Message: 6 Date: Tue, 11 Sep 2007 13:52:33 -0400 From: "Robert Richmond" Subject: [Histonet] Re: Lymph node fixative/enhance solutions To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Cindi Robinson, HT (ASCP) in South Dakota asks: >>Our pathologists would like us to start using a solution to help with the dissection and identification of lymph nodes at gross. - We do use Pen-Fix but it doesn't dissolve the fat like the paths would like.<< And Dana Dittus at Polysciences notes: >>Polysciences,Inc manufactures Hartmanns Fixative which turns lymph nodes white. For more info see www.polysciences.com << It's easy and cheap to prepare Davidson's fixative (same as Hartmann's fixative) - mix 3 parts water, 3 parts reagent alcohol, 2 parts strong (37%) formalin, not the buffered kind, and 1 part glacial acetic acid. Color it with eosin if you want to. When Dr. William Hartmann (later the chair of the American Board of Pathology) was at Johns Hopkins in the early 1960's, Dr. Victor McCusick (the medical geneticist, later chair of medicine at Hopkins) asked him to introduce the fixative for Barr bodies (sex chromatin bodies) that Moore and Barr had specified in their original articles on Barr bodies around 1954. Moore and Barr specified "modified Davidson's fixative", which Bill Hartmann introduced. It came into rather widespread use at Hopkins, and Hartmann's name stuck to it. I think the name should not be used, because of possible disastrous confusion with Hartmann's solution, lactated Ringer's solution, introduced into pediatric practice in the 1930's by Dr. Alexis Hartmann Sr. at Washington University in St.Louis. Dr. Alexis Hartmann was one of my clinical teachers, right before he retired around 1964 (sorry about all this name dropping). John Kiernan and I discussed Davidson's fixative in this forum several years ago. He noted that it is not really a rational formula. He hoped to have the time to research the fixative in Davidson's papers, since it seems never to have been published. Getting back to the actual subject, I've used Dissect Aid (Decal Corporation) with good results - got a bottle of it on the shelf right now, in fact. In my brief experience with Penn-Fix, it did not disclose lymph nodes well. Let me support your pathologists very strongly in this endeavor. Just one positive lymph node upstages a colon cancer and makes chemotherapy mandatory. And in colon cancer, those postive lymph nodes can be the size of pinheads. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 7 Date: Tue, 11 Sep 2007 12:02:57 -0500 From: "Joe Nocito" Subject: Re: [Histonet] Worlds Largest Paraffin Dispenser To: "Douglas D Deltour" , Message-ID: <000001c7f4a9$fcd58310$0202a8c0@yourxhtr8hvc4p> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original I know TBS and ThermoFisher have 5 gallon pots. How big do you need? I'm getting visions of hot paraffin in a hot tub. Any vendors want to try that for Denver? I mean, it will be the end of October. Joe ----- Original Message ----- From: "Douglas D Deltour" To: Sent: Tuesday, September 11, 2007 11:49 AM Subject: [Histonet] Worlds Largest Paraffin Dispenser >I am looking for a paraffin dispenser that holds a LARGE amount of >paraffin. > My requests for a hot tub that I can fill with paraffin have been denied > for > some reason. Anyone know of a manufacturer of these large dispensers? > > > > Douglas D. Deltour HT(ASCP) > > Histology Manager > > Professional Pathology Services, PC > > One Science Court > > Suite 200 > > Columbia, SC 29203 > > Office (803)252-1913 > > Fax (803)254-3262 > > Doug@ppspath.com > > ***************************************************** > > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of the individual or entity to > which it is addressed and may contain information that is privileged, > confidential and exempt from disclosure under applicable law. If the > reader > of this message is not the intended recipient, you are hereby notified > that > any dissemination, distribution, or copying of this communication is > strictly prohibited by law. If you have received this communication in > error, please notify me immediately. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Tue, 11 Sep 2007 12:58:31 -0700 From: "Mindy Johnson" Subject: [Histonet] Looking for a specific type of slide tray..... To: "'HistoNet'" Message-ID: <007301c7f4ae$212c80c0$63858240$@ucsd.edu> Content-Type: text/plain; charset="us-ascii" Hi All- We are looking for some trays that hold slides. We currently have about 3 and I need more. They look old school and I have no clue the technical term for them. But, they hold about 50 slides (5 rows hold about 10 each), are flat on one side and the other is divided into the 5 rows, the material is made of that stuff that resembles a wood like fiberboard. They are extremely handy for our lab and good for after cutting I put in the incubator to make sure they are totally dry before staining. Anyone have any idea where I can find something similar to this? Thank you so much for your help! Mindy ------------------------------ Message: 9 Date: Tue, 11 Sep 2007 15:00:52 -0500 From: "Bernice Frederick" Subject: RE: [Histonet] Worlds Largest Paraffin Dispenser To: "'Joe Nocito'" , "'Douglas D Deltour'" , Message-ID: <001a01c7f4ae$77f76fa0$d00f7ca5@lurie.northwestern.edu> Content-Type: text/plain; charset="us-ascii" One BIG candle for someones birthday!!!!!!!!! Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Tuesday, September 11, 2007 12:03 PM To: Douglas D Deltour; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Worlds Largest Paraffin Dispenser I know TBS and ThermoFisher have 5 gallon pots. How big do you need? I'm getting visions of hot paraffin in a hot tub. Any vendors want to try that for Denver? I mean, it will be the end of October. Joe ----- Original Message ----- From: "Douglas D Deltour" To: Sent: Tuesday, September 11, 2007 11:49 AM Subject: [Histonet] Worlds Largest Paraffin Dispenser >I am looking for a paraffin dispenser that holds a LARGE amount of >paraffin. > My requests for a hot tub that I can fill with paraffin have been denied > for > some reason. Anyone know of a manufacturer of these large dispensers? > > > > Douglas D. Deltour HT(ASCP) > > Histology Manager > > Professional Pathology Services, PC > > One Science Court > > Suite 200 > > Columbia, SC 29203 > > Office (803)252-1913 > > Fax (803)254-3262 > > Doug@ppspath.com > > ***************************************************** > > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of the individual or entity to > which it is addressed and may contain information that is privileged, > confidential and exempt from disclosure under applicable law. If the > reader > of this message is not the intended recipient, you are hereby notified > that > any dissemination, distribution, or copying of this communication is > strictly prohibited by law. If you have received this communication in > error, please notify me immediately. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Tue, 11 Sep 2007 12:05:43 -0800 From: "Kriederman, Ben" Subject: [Histonet] Opinion on Microm automated microtomes? To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Does anyone have an opinion on the microm hm355s automated microtome? When it comes to cutting hard tissues we are getting thick and thin, any suggestions? Thick and Thin in Alaska DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. ------------------------------ Message: 11 Date: Tue, 11 Sep 2007 13:10:54 -0700 (PDT) From: Larry Woody Subject: Re: [Histonet] Worlds Largest Paraffin Dispenser To: histonet@lists.utsouthwestern.edu Message-ID: <308814.42020.qm@web53605.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I've heard of people getting into their work before but embedding yourself? --------------------------------- Be a better Heartthrob. Get better relationship answers from someone who knows. Yahoo! Answers - Check it out. ------------------------------ Message: 12 Date: Tue, 11 Sep 2007 15:34:29 -0500 From: "Horn, Hazel V" Subject: RE: [Histonet] Looking for a specific type of slide tray..... To: "Mindy Johnson" , "HistoNet" Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB7335@EMAIL.archildrens.org> Content-Type: text/plain; charset=us-ascii I know I ordered some of these trays before and the company was called brain research labs? But I looked on the internet and cannot find anything. The flats were trimmed in wood with a white background. Each row held 10 slides and there are 5 rows on the board. Maybe someone else can help you. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mindy Johnson Sent: Tuesday, September 11, 2007 2:59 PM To: 'HistoNet' Subject: [Histonet] Looking for a specific type of slide tray..... Hi All- We are looking for some trays that hold slides. We currently have about 3 and I need more. They look old school and I have no clue the technical term for them. But, they hold about 50 slides (5 rows hold about 10 each), are flat on one side and the other is divided into the 5 rows, the material is made of that stuff that resembles a wood like fiberboard. They are extremely handy for our lab and good for after cutting I put in the incubator to make sure they are totally dry before staining. Anyone have any idea where I can find something similar to this? Thank you so much for your help! Mindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== ------------------------------ Message: 13 Date: Tue, 11 Sep 2007 13:38:56 -0700 From: "Ford, Judi" Subject: RE: [Histonet] Opinion on Microm automated microtomes? To: "Kriederman, Ben" , Message-ID: Content-Type: text/plain; charset="us-ascii" I've been working with that model for quite sometime now and once in awhile I get thick and thin sections. Usually it happens when something is loose or I'm cutting in the middle of the blade. I tend to cut on either end of the knife blade, avoiding the middle of the blade holder when I can. Check the knife angle (9 or 10). Also, try checking the block to see that it is properly seated in the chuck and tight. Hope this helps. Good luck. Judi Ford Palo Alto, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kriederman, Ben Sent: Tuesday, September 11, 2007 1:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Opinion on Microm automated microtomes? Does anyone have an opinion on the microm hm355s automated microtome? When it comes to cutting hard tissues we are getting thick and thin, any suggestions? Thick and Thin in Alaska DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Tue, 11 Sep 2007 14:15:42 -0700 From: "Mindy Johnson" Subject: RE: [Histonet] Looking for a specific type of slide tray..... To: "'Horn, Hazel V'" , "'HistoNet'" Message-ID: <007e01c7f4b8$e9c5c280$bd514780$@ucsd.edu> Content-Type: text/plain; charset="iso-8859-1" Yes it is Brain Research Labs! They are exactly what I was looking for! THANK YOU SOOOO MUCH!! ?Mindy A Johnson SRA II UCSD - School of Medicine Medical Teaching Labs -----Original Message----- From: Horn, Hazel V [mailto:HornHV@archildrens.org] Sent: Tuesday, September 11, 2007 1:34 PM To: Mindy Johnson; HistoNet Subject: RE: [Histonet] Looking for a specific type of slide tray..... I know I ordered some of these trays before and the company was called brain research labs? But I looked on the internet and cannot find anything. The flats were trimmed in wood with a white background. Each row held 10 slides and there are 5 rows on the board. Maybe someone else can help you. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mindy Johnson Sent: Tuesday, September 11, 2007 2:59 PM To: 'HistoNet' Subject: [Histonet] Looking for a specific type of slide tray..... Hi All- We are looking for some trays that hold slides. We currently have about 3 and I need more. They look old school and I have no clue the technical term for them. But, they hold about 50 slides (5 rows hold about 10 each), are flat on one side and the other is divided into the 5 rows, the material is made of that stuff that resembles a wood like fiberboard. They are extremely handy for our lab and good for after cutting I put in the incubator to make sure they are totally dry before staining. Anyone have any idea where I can find something similar to this? Thank you so much for your help! Mindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------------- -- The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================ == ------------------------------ Message: 15 Date: Tue, 11 Sep 2007 18:32:37 -0500 From: "Rebecca Johnson" Subject: [Histonet] Special Stainers To: "histonet" Message-ID: <011a01c7f4cc$09fefcc0$7b48f9d8@CHURCH> Content-Type: text/plain; charset="iso-8859-1" What special stainers is everyone using? I now have a Ventana Nexus. Bought in 1999. I know this is old, but it has been maintained well. We have had problems with it several times over the years. Mostly with sliver stains or uneven staining and no staining. I would like to know what others are using and your experiences are. Thanks for all you help. raj ------------------------------ Message: 16 Date: Tue, 11 Sep 2007 18:39:37 -0500 From: "Rebecca Johnson" Subject: [Histonet] Special Stainers To: "histonet" Message-ID: <012801c7f4cd$045aa1b0$7b48f9d8@CHURCH> Content-Type: text/plain; charset="iso-8859-1" Need advice on special stainers everyone is using. We now have Ventana Nexus special stainer.Bought in 1999. I know this is old but it has been maintained well. We have always had a service contract with Ventana. Have had problems over the years with the silver stains, no staining and uneven staining. Now they want to tell me that this is normal with any company. For the amount of money these stainers cost that is just little more than I am willing to believe. Thanks for all you help. raj ------------------------------ Message: 17 Date: Tue, 11 Sep 2007 19:00:33 -0500 From: "Rebecca Johnson" Subject: [Histonet] Histnet Special Stainers To: "histonet" Message-ID: <017101c7f4cf$f0967bb0$7b48f9d8@CHURCH> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Need advice on what special stainers everyone is using. The good and bad. We are now using Ventana Nexus special stainer. Bought in 1999. Not real happy with what I am hearing about all stainers. I was told today by a manager that all stainers have problems a couple times a year. We have had uneven staining, no staining and problems with the silver stain. For what they cost and the cost of the maintenance contracts, this is hard for me to believe. What is your experience with the stainers. Thanks raj ------------------------------ Message: 18 Date: Tue, 11 Sep 2007 20:33:58 -0300 From: "ribonukle" Subject: Re: [Histonet] Opinion on Microm automated microtomes? To: Message-ID: <01e801c7f4cc$3b8adb10$590215ac@Pentium4> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original I have a similar model and also has that problem, thick and thin with all very good adjusted.they have already become unstuck and broken several things This microm Microtome have low quality Hope this helps. Ribo ----- Original Message ----- From: "Ford, Judi" To: "Kriederman, Ben" ; Sent: Tuesday, September 11, 2007 5:38 PM Subject: RE: [Histonet] Opinion on Microm automated microtomes? I've been working with that model for quite sometime now and once in awhile I get thick and thin sections. Usually it happens when something is loose or I'm cutting in the middle of the blade. I tend to cut on either end of the knife blade, avoiding the middle of the blade holder when I can. Check the knife angle (9 or 10). Also, try checking the block to see that it is properly seated in the chuck and tight. Hope this helps. Good luck. Judi Ford Palo Alto, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kriederman, Ben Sent: Tuesday, September 11, 2007 1:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Opinion on Microm automated microtomes? Does anyone have an opinion on the microm hm355s automated microtome? When it comes to cutting hard tissues we are getting thick and thin, any suggestions? Thick and Thin in Alaska DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Wed, 12 Sep 2007 10:01:05 +1000 From: "Anthony Reilly" Subject: Re: [Histonet] Opinion on Microm automated microtomes? To: , Message-ID: Content-Type: text/plain; charset="us-ascii" I agree. I have 2 old Microms purchased in the early nineties which were very good on purchase and are still going strong however we have another that was purchased about 2 years ago and there is a marked decrease in the sectioning ability. Even the physical appearance of the instument is notably inferior. Some blocks will cut thick and thin for no apparent reason. They do not even have to be hard tissue. regards Tony Reilly Chief Scientist Anatomical Pathology QHPS-Prince Charles Hospital Rode Rd Chermside Q 4032 Australia Ph: 07 3139 4543 Fax: 07 3193 4546 tony_reilly@health.qld.gov.au >>> "ribonukle" 09/12/07 9:33 am >>> I have a similar model and also has that problem, thick and thin with all very good adjusted.they have already become unstuck and broken several things This microm Microtome have low quality Hope this helps. Ribo ----- Original Message ----- From: "Ford, Judi" To: "Kriederman, Ben" ; Sent: Tuesday, September 11, 2007 5:38 PM Subject: RE: [Histonet] Opinion on Microm automated microtomes? I've been working with that model for quite sometime now and once in awhile I get thick and thin sections. Usually it happens when something is loose or I'm cutting in the middle of the blade. I tend to cut on either end of the knife blade, avoiding the middle of the blade holder when I can. Check the knife angle (9 or 10). Also, try checking the block to see that it is properly seated in the chuck and tight. Hope this helps. Good luck. Judi Ford Palo Alto, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kriederman, Ben Sent: Tuesday, September 11, 2007 1:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Opinion on Microm automated microtomes? Does anyone have an opinion on the microm hm355s automated microtome? When it comes to cutting hard tissues we are getting thick and thin, any suggestions? Thick and Thin in Alaska DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/ received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. 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Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. **************************************************************** ------------------------------ Message: 20 Date: Wed, 12 Sep 2007 15:28:32 +1000 From: Cathy Malcontenti-Wilson Subject: [Histonet] co-localisation within a cell using DAB and Vulcan Fast Red To: Message-ID: <6.2.1.2.2.20070912152507.0421f230@mail.staff.unimelb.edu.au> Content-Type: text/plain; charset="us-ascii"; format=flowed Dear Histonetters, Does anyone have experience with co-localisation of the chromogens DAB and Vulcan Fast Red in an immunohistochemical stain? I have just done a double stain and I would like to know what I should I expect if the two antigens I have stained are co-localised in the one cell? Would it to look red or orange or what?? Much appreciated, Thanks Cathy Malcontenti-Wilson ------------------------------ Message: 21 Date: Wed, 12 Sep 2007 10:58:54 -0400 From: Caroline Bass Subject: [Histonet] AO 860 sliding microtome To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Hi guys, So I've inherited an American Optical 860 sliding microtome. I consider this quite a find since it is my all time favorite for slicing fixed brains. However, it has been packed away for quite a while and seems to be a bit gummed up and is in parts. Does anyone have suggestions on how to get this back into working order. I don't suppose there is a manual floating around? Any general suggestions for degreasing to remove the stick points. How about a location for spare parts, particularly dry ice stages and maybe a disposable blade holder? Any and all suggestions are appreciated. Thanks, Caroline Bass ------------------------------ Message: 22 Date: Wed, 12 Sep 2007 11:29:57 -0400 From: "Marcia Funk" Subject: [Histonet] QC labeling To: histonet@lists.utsouthwestern.edu Message-ID: <46E7BFA5.9B87.00AC.0@mercyhealth.com> Content-Type: text/plain; charset=us-ascii Histo Friends, I have a few questions I would like to see how labs are following through with labeling errors? Case number placed on slide during sectioning. Slide is then mislabeled handed off to pathologist. Pathologist discovers the error. Slide is labeled incorrectly. Wrong label placed on slide during labeling. Pathologist discovers mislabeld slide Errors are place on QC log at the end of day. These are errors that are caught in the department by the tech or pathologist. Do most labs keep a log with errors by tech and how many errors impact there eval ? I would appreciate your imput. Mrf ------------------------------ Message: 23 Date: Wed, 12 Sep 2007 08:31:03 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] AO 860 sliding microtome To: Caroline Bass , histonet@lists.utsouthwestern.edu Message-ID: <753832.79448.qm@web61225.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Caroline: I learned to cut in one of those (in 1952) and I still consider this model of horizontal microtome the finest ever built, specially with its clever vertical automatic feeding mechanism. IF you have mechanical dextereity, it is not difficult to put it back in working conditoins. As you can see, almost every part is screwed and even the feeding mechanisms is like a single unit. You will have to unscrew it all and place each component in a bucket with mineral spirits or any other grease solvent. Even kerosene will work. Leave the parts in the degreaser for at least 3 days, you will see how the old grease will dissolve and color the degreaser. Air dry it (presurized air). Repeat the cleaning (degreasing) if it still looks greasy. After you see each part is clean and the nickel chorme is gleaming and sparkling, grease it up (with the same type of microtome grease you would use for any modern microtome; I always used the Carl Jung light oil No. 405; Leica has also a very fine light oil,called "Schlitten-bahnenol Nr. 601". The feeding trigger has to be lubricated with light oil, because its return to vertical is caused by gravity, and cannot be stuck in any way. The screw for the feeding mechanism has to receive solid grease (Jelly lubrican), I always used the Lipshaw No.294 This type of maintenance I used to do, at least annually. Enjoy your cleaning! Ren? J. Caroline Bass wrote: Hi guys, So I've inherited an American Optical 860 sliding microtome. I consider this quite a find since it is my all time favorite for slicing fixed brains. However, it has been packed away for quite a while and seems to be a bit gummed up and is in parts. Does anyone have suggestions on how to get this back into working order. I don't suppose there is a manual floating around? Any general suggestions for degreasing to remove the stick points. How about a location for spare parts, particularly dry ice stages and maybe a disposable blade holder? Any and all suggestions are appreciated. Thanks, Caroline Bass _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better Globetrotter. Get better travel answers from someone who knows. Yahoo! Answers - Check it out. ------------------------------ Message: 24 Date: Wed, 12 Sep 2007 08:39:55 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] QC labeling To: Marcia Funk , histonet@lists.utsouthwestern.edu Message-ID: <531579.50543.qm@web61222.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Marcia: Short answer, yes! I used to keep a file for each HT, and in it everything was written down: the good, the bad, the uggly; trainings, commendations, attendance, you name it. It was updated everytime anything worth mentioning took place, from commendations from the pathologists for good sections, staining, whatever; as well as any mistakes, not only dealing with mislabelling, but with poor quality of sections, floaters, as I wrote, every thing. Come the evaluation day all those things, good and bad, all documented AT THE MOMENT THEY HAPPENED, not after the fact, we weighed and most certainly affected the evaluation, either positively or negatively. Once that evaluation was completed, signed, agreed or disagreed, that annual file was incorporated to the personal file, and a NEW file was opened for the next evaluation period. Since all issues were addressed immediately they happened or were found, I never had a single case of a HT saying: "I did not know about that", they knew and received retraining if necessary. Ren? J. Marcia Funk wrote: Histo Friends, I have a few questions I would like to see how labs are following through with labeling errors? Case number placed on slide during sectioning. Slide is then mislabeled handed off to pathologist. Pathologist discovers the error. Slide is labeled incorrectly. Wrong label placed on slide during labeling. Pathologist discovers mislabeld slide Errors are place on QC log at the end of day. These are errors that are caught in the department by the tech or pathologist. Do most labs keep a log with errors by tech and how many errors impact there eval ? I would appreciate your imput. Mrf _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Building a website is a piece of cake. Yahoo! Small Business gives you all the tools to get online. ------------------------------ Message: 25 Date: Wed, 12 Sep 2007 11:45:47 -0500 From: mari.ann.mailhiot@leica-microsystems.com Subject: Re: [Histonet] AO 860 sliding microtome To: Caroline Bass Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII Hi Caroline Please contact me here at Leica about the AO860 sliding microtome. Rene has offered an excellent clean application for this unit. Thanks Renee. Best Regards Mari Ann Mailhiot BA HT ASCP Application Specialist Leica Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 46, Issue 18 **************************************** From Joanne.Malinowski <@t> crl.com Wed Sep 12 16:17:02 2007 From: Joanne.Malinowski <@t> crl.com (Malinowski, Joanne O,) Date: Wed Sep 12 16:17:14 2007 Subject: [Histonet] Recall: Spur Resin Message-ID: <3E94391DD24ECE418DCBA5EF74BA29940B36B7@shr-exch1.na01.crl.com> Malinowski, Joanne O, would like to recall the message, "Spur Resin". From Joanne.Malinowski <@t> crl.com Wed Sep 12 16:19:01 2007 From: Joanne.Malinowski <@t> crl.com (Malinowski, Joanne O,) Date: Wed Sep 12 16:19:12 2007 Subject: [Histonet] Spur Resin Message-ID: <3E94391DD24ECE418DCBA5EF74BA29940B36B9@shr-exch1.na01.crl.com> Hello Plastic People, Could anyone share their recipe for using spur resin to produce plastic sections? Thank you all in advance for offering any help. Best regards, Joanne Joanne Malinowski,HT ASCP Plastics Lab Manager, Medical Devices Division Charles River Laboratories Pathology Associates 15 Worman's Mill Court, Suite I Frederick, Maryland 21701 Phone 301-624-2034 Fax 301-663-8994 Email: joanne.malinowski@us.crl.com -----Original Message----- Joanne Malinowski,HT ASCP Plastics Lab Manager, Medical Devices Division Charles River Laboratories Pathology Associates 15 Worman's Mill Court, Suite I Frederick, Maryland 21701 Phone 301-624-2034 Fax 301-663-8994 Email: joanne.malinowski@us.crl.com From immrstambo <@t> hotmail.com Wed Sep 12 16:25:55 2007 From: immrstambo <@t> hotmail.com (Christine Tambasco) Date: Wed Sep 12 16:26:11 2007 Subject: [Histonet]immuno stainers In-Reply-To: <001a01c7f576$53721540$6401a8c0@yourvp7x3s9ctm> Message-ID: Anybody have an experiences with the Nemisis from BioCare? Would appreciate any feed back. Thanks Christine Tambasco, HT (ASCP) St Marys Hospital, Amsterdam NY ______________________________________________________________ From: "Mike Schlicht" To: Subject: Re: [Histonet] Special Stainers Date: Wed, 12 Sep 2007 12:51:34 -0700 >We are currently using the DAKO Artisan for silver stains, and have >some problems with uneven staining every now and then, usually when >the dispensers are close to being empty, so we stop using the kit >when there are 5 tests remaining to help reduce some of the >problems. We had a demo of the Nexus stainer, and found that the >result were about the same. >Mike >San Luis Obispo, CA > > >>Message: 15 >>Date: Tue, 11 Sep 2007 18:32:37 -0500 >>From: "Rebecca Johnson" >>Subject: [Histonet] Special Stainers >>To: "histonet" >>Message-ID: <011a01c7f4cc$09fefcc0$7b48f9d8@CHURCH> >>Content-Type: text/plain; charset="iso-8859-1" >> >>What special stainers is everyone using? I now have a Ventana >>Nexus. Bought in 1999. I know this is old, but it has been >>maintained well. We have had problems with it several times over >>the years. Mostly with sliver stains or uneven staining and no >>staining. I would like to know what others are using and your >>experiences are. Thanks for all you help. >>raj >> > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ [1]Kick back and relax with hot games and cool activities at the Messenger Café. References 1. http://g.msn.com/8HMBENUS/2740??PS=47575 From mmccoy100 <@t> gmail.com Wed Sep 12 16:30:59 2007 From: mmccoy100 <@t> gmail.com (Michelle McCoy) Date: Wed Sep 12 16:31:12 2007 Subject: [Histonet] IHC and state requirements Message-ID: <25355ef80709121430v7a719e0cvb28fd694c0341611@mail.gmail.com> I am a Florida licensed HT (ASCP). I'm planning to apply for work out of the state and have seen jobs titled "Histotechnologist" and was curious if I am eligible to apply for jobs with this title --if this term is only applied for HTL credential holders. Or if it is applied to anyone who has histotechnology experience (I believe someone had mentioned you dont have to have HT or HTL credentials to work in the field in many states). Some job listings state IHC duties and I'm guessing I can perform them as an HT (ASCP) if I have experience or they are willing to train in IHC (unless otherwise indicated that they are only looking for HTL candidates) Thanks for any clarification on this. Michelle From jnocito <@t> satx.rr.com Wed Sep 12 19:09:31 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Sep 12 19:09:45 2007 Subject: [Histonet] IHC and state requirements References: <25355ef80709121430v7a719e0cvb28fd694c0341611@mail.gmail.com> Message-ID: <002501c7f59a$5c5a50e0$0202a8c0@yourxhtr8hvc4p> Michelle, in Texas, things are a little less slack. In some parts you do not have to be ASCP registered. As much as some people would like to be. We have been fighting for a licensure for years, but to no avail. In a nutshell (I prefer peanuts) rules are lax in Texas. JTT ----- Original Message ----- From: "Michelle McCoy" To: Sent: Wednesday, September 12, 2007 4:30 PM Subject: [Histonet] IHC and state requirements >I am a Florida licensed HT (ASCP). I'm planning to apply for work out of >the > state and have seen jobs titled "Histotechnologist" and was curious if I > am > eligible to apply for jobs with this title --if this term is only applied > for HTL credential holders. Or if it is applied to anyone who has > histotechnology experience (I believe someone had mentioned you dont have > to > have HT or HTL credentials to work in the field in many states). Some job > listings state IHC duties and I'm guessing I can perform them as an HT > (ASCP) if I have experience or they are willing to train in IHC (unless > otherwise indicated that they are only looking for HTL candidates) Thanks > for any clarification on this. > > Michelle > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dcojita <@t> tampabay.rr.com Wed Sep 12 19:15:23 2007 From: dcojita <@t> tampabay.rr.com (dcojita@tampabay.rr.com) Date: Wed Sep 12 19:18:40 2007 Subject: [Histonet] Clia License In-Reply-To: <25355ef80709121430v7a719e0cvb28fd694c0341611@mail.gmail.com> Message-ID: Has anyone heard that there be new CLIA regs coming out in November regarding techs who gross? I am familiar with the one that came out last January, but I heard there are newer, more stringent regulations on the way for grossing. From JMacDonald <@t> mtsac.edu Thu Sep 13 00:17:12 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Sep 13 00:17:25 2007 Subject: [Histonet] Job opening for histology assistant/associate Message-ID: Information about our company can be found on this website: www.siriontherapeutics.com Sirion Therapeutics Research Assistant/Associate Location: San Diego, California-San Diego 92121 Position type: Full time Required education: Bachelor's Degree Area(s) of expertise desired: Histology, Cell Biology, Cell Culture, Laboratory Description Sirion Therapeutics is an ophthalmic-focused company dedicated to the development and commercialization of innovative eye products to address unmet clinician and patient needs. Sirion Therapeutics was founded with a mission to protect and preserve our most precious gift: the gift of sight. Our dedicated team of employees pursues this mission with a passion and commitment to assist those suffering with eye diseases and the healthcare professionals who treat them. The successful candidate will work as a member of a project team reporting to a senior staff member. The candidate should be familiar with histological techniques including tissue collection, processing, sectioning and staining/developing procedures (e.g., immunohistochemistry). Experience with bright-field and fluorescence microscopy is also desired. The candidate will be expected to collect, organize and analyze data. Preference will be given to candidates who also possess some level of expertise in establishing and maintaining mammalian cell culture. The candidate will also be responsible for preparing reagents and chemical solutions, performing specialized laboratory procedures as required and maintaining laboratory equipment and supplies. Requirements . We are seeking a highly motivated individual with excellent verbal and written communication skills. The successful candidate will be detail oriented, organized and must be able to work independently as well as in a team environment. EDUCATION/EXPERIENCE: A B.S. degree in Biology or a related field with a minimum of 1-3 years of laboratory experience in either academia or industry is desired. Silvia N. M. Reid, Ph.D. Senior Scientist SIRiON Therapeutics - www.siriontherapeutics.com P: 858.875.9258 F: 858.875.9251 E: sreid@siriontherapeutics.com 11408 Sorrento Valley Rd San Diego, CA 92121 From mark.webber <@t> nuigalway.ie Thu Sep 13 03:37:31 2007 From: mark.webber <@t> nuigalway.ie (Webber, Mark) Date: Thu Sep 13 03:39:45 2007 Subject: [Histonet] Antibody for ATM (Ataxia telangiectasia mutated) in paraffin embedded tissue Message-ID: <6B017AD2AE2F6F489087FC986588136B02BAAED5@EVS1.ac.nuigalway.ie> We are having trouble getting antibodies to ATM (Ataxia telangiectasia mutated) to work in formalin fixed paraffin embedded (FFPE) human tissue. Does anyone have experience and/or recommendations for an antibody that works and the preferred method of staining Thanks Mark Webber Department of Pathology National University of Ireland, Galway From oshel1pe <@t> cmich.edu Thu Sep 13 07:23:36 2007 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Thu Sep 13 07:23:58 2007 Subject: [Histonet] Spur Resin In-Reply-To: <3E94391DD24ECE418DCBA5EF74BA29940B36B5@shr-exch1.na01.crl.com> References: <3E94391DD24ECE418DCBA5EF74BA29940B36B5@shr-exch1.na01.crl.com> Message-ID: Joanne, First, a couple of questions: Hard, soft, or "standard"? Standard is usual (oddly enough), hard is for harder tissues -- not bone, but tough tissues. I haven't found a use for soft, but there's one out there somewhere. Second: One of the major components of Spurr's ERL 4206 was discontinued last year (too carcinogenic) and replaced with ERL 4221. The vendors were claiming a 1 to 1 replacement of 4221 for 4206, but it's not true. I have an article from Microscopy Today in pdf format I can send to anyone who would like one. Briefly, the recipes are: **all values in grams** to make 10g Standard ERL 4221 4.10g NSA 5.90g DER 736 1.43g DMAE 0.10g Hard ERL 4221 4.10g NSA 5.90g DER 736 0.95g DMAE 0.10g Soft ERL 4221 4.10g NSA 5.90g DER 736 1.90g DMAE 0.10g These were worked out by E. Ann Ellis at Texas A&M. Phil >Hello Plastic People, > Could anyone share their recipe for using spur resin to produce >plastic sections? Thank you all in advance for offering any help. >Best regards, >Joanne > >Joanne Malinowski,HT ASCP >Plastics Lab Manager, Medical Devices Division >Charles River Laboratories >Pathology Associates >15 Worman's Mill Court, Suite I >Frederick, Maryland 21701 > >Phone 301-624-2034 >Fax 301-663-8994 >Email: joanne.malinowski@us.crl.com -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 From LSebree <@t> uwhealth.org Thu Sep 13 08:09:40 2007 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Thu Sep 13 08:09:57 2007 Subject: [Histonet]immuno stainers In-Reply-To: Message-ID: Please post replies to Histonet. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Christine Tambasco Sent: Wednesday, September 12, 2007 4:26 PM To: histomike@charter.net; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet]immuno stainers Anybody have an experiences with the Nemisis from BioCare? Would appreciate any feed back. Thanks Christine Tambasco, HT (ASCP) St Marys Hospital, Amsterdam NY ______________________________________________________________ From: "Mike Schlicht" To: Subject: Re: [Histonet] Special Stainers Date: Wed, 12 Sep 2007 12:51:34 -0700 >We are currently using the DAKO Artisan for silver stains, and have >some problems with uneven staining every now and then, usually when >the dispensers are close to being empty, so we stop using the kit >when there are 5 tests remaining to help reduce some of the >problems. We had a demo of the Nexus stainer, and found that the >result were about the same. >Mike >San Luis Obispo, CA > > >>Message: 15 >>Date: Tue, 11 Sep 2007 18:32:37 -0500 >>From: "Rebecca Johnson" >>Subject: [Histonet] Special Stainers >>To: "histonet" >>Message-ID: <011a01c7f4cc$09fefcc0$7b48f9d8@CHURCH> >>Content-Type: text/plain; charset="iso-8859-1" >> >>What special stainers is everyone using? I now have a Ventana >>Nexus. Bought in 1999. I know this is old, but it has been >>maintained well. We have had problems with it several times over >>the years. Mostly with sliver stains or uneven staining and no >>staining. I would like to know what others are using and your >>experiences are. Thanks for all you help. >>raj >> > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ [1]Kick back and relax with hot games and cool activities at the Messenger Caf?. References 1. http://g.msn.com/8HMBENUS/2740??PS=47575 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Thu Sep 13 08:10:40 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Sep 13 08:11:03 2007 Subject: [Histonet] IHC and state requirements In-Reply-To: <002501c7f59a$5c5a50e0$0202a8c0@yourxhtr8hvc4p> Message-ID: <004801c7f607$7e8a8640$d00f7ca5@lurie.northwestern.edu> I like almonds myself. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, September 12, 2007 7:10 PM To: Michelle McCoy; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC and state requirements Michelle, in Texas, things are a little less slack. In some parts you do not have to be ASCP registered. As much as some people would like to be. We have been fighting for a licensure for years, but to no avail. In a nutshell (I prefer peanuts) rules are lax in Texas. JTT ----- Original Message ----- From: "Michelle McCoy" To: Sent: Wednesday, September 12, 2007 4:30 PM Subject: [Histonet] IHC and state requirements >I am a Florida licensed HT (ASCP). I'm planning to apply for work out of >the > state and have seen jobs titled "Histotechnologist" and was curious if I > am > eligible to apply for jobs with this title --if this term is only applied > for HTL credential holders. Or if it is applied to anyone who has > histotechnology experience (I believe someone had mentioned you dont have > to > have HT or HTL credentials to work in the field in many states). Some job > listings state IHC duties and I'm guessing I can perform them as an HT > (ASCP) if I have experience or they are willing to train in IHC (unless > otherwise indicated that they are only looking for HTL candidates) Thanks > for any clarification on this. > > Michelle > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From simmonsca <@t> upmc.edu Thu Sep 13 08:57:06 2007 From: simmonsca <@t> upmc.edu (Simmons, Christopher) Date: Thu Sep 13 08:57:18 2007 Subject: [Histonet] Ventana Nexus Special Stainer issues Message-ID: <88D6F512ADC2CB428B90845D2D1388E303A5BB51@1upmc-msx6.acct.upmchs.net> I have read many comments recently regarding silver staining issues on the Nexus special stainer from Ventana. I met yesterday withthe USA manager for special stains from Ventana. She assures me that the issue is being researched. I am having hit and miss staining here with the Grocott and Jones stains. Decontamination seems to help for a few days then the issue keeps popping up. We are about to pull the 2 stains from the stainer and do them by hand. We have 6 stainers here, losing this stain will put a large burden on the technicians. Hope this helps to validate the issues others are having with the stainer as well. Chris Simmons Lead Tech UPMC Presbyterian/Shadyside 412.647.7660 From ROrr <@t> enh.org Thu Sep 13 09:14:52 2007 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Thu Sep 13 09:15:03 2007 Subject: [Histonet] Immuno stainers Message-ID: ---- Anybody have an experiences with the Nemisis from BioCare? Would appreciate any feed back. Thanks Christine Tambasco, HT (ASCP) St Marys Hospital, Amsterdam NY HI Christine, I've been working with the Biocare team and using the Nemesis stainer since its introduction a few years ago. I now supervise a busy IHC lab. The Nemesis Stainer can accommodate any reagent, and is in my opinion very flexible. I need to try out novel antibodies for our Research Institute and I need to be able to run any kind of detection as well as chromogen. >From the clinical aspect, it can accommodate a higher volume of slides with the cost per slide being relatively low. This is comparing it to the cost per slide of another stainer I use that has software and reagents that must be bought from that instrument company. Our lab relies on instrumentation that bar codes the slides as well as the reagents. The Nemesis has this feature and can read the slides and reagents very quickly to get the run started fast. It also checks the volumes of the reagents before the run, so this is nice for our lab, too. Since I've worked with the Biocare reagents, I have a high level of confidence in them. I use the Mach 3 and 4 detection systems and the Automated wash buffer on all of the slides I run on the Nemesis. In my experience the Biocare products have demonstrated for me high quality and consistent results. Any problems I have encountered have mostly been due to my unfamiliarity with a reagent or protocol... To that I have turned to Biocare for support and I have the highest confidence in Biocare's Tech Support group. I have had a few adjustments to my instrument over the years and I have experienced immediate response to all of my requests for service. Biocare is proactive with preventative maintenance. They schedule with me before I need to call them and ask for it. My lab would be sorely hampered without this work horse processing an average 150 slides/day. If you'd like, I'll be glad to run a few of your slides for you on this system. Hope this helps. Becky Orr CLA,HT(ASCP)QIHC Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 From kimtournear <@t> yahoo.com Thu Sep 13 09:26:43 2007 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Thu Sep 13 09:26:55 2007 Subject: [Histonet] RE: IHC and State requirements Message-ID: <40413.11554.qm@web50603.mail.re2.yahoo.com> Hi Michelle, A lot employers will except either HT or HTL. And of course, how much experience you have under your belt is also a big consideration. I think a lot of employers are finding out just how hard it is to find techs (registered or not) that can do the job and do it well, although there are a few states with their own state requirements which would require the tech to be ASCP certified either way. I have found that being an HTL vs HT doesn't necessarily mean that one or the other can do the job better...I know a few HTL's that were grandfathered in and have done only a handful IHCs in their whole career, can't trouble shoot anything, and produce poor quality slides and yet they hold the title of an HTL and the same goes for the HTs...(makes you wonder how they keep a job)...LOL....and I have taught and worked with students right out of college programs that were absolutely awesome and knew their stuff....go figure.....I say go for it.....Good Luck.... Kim Tournear, HT (ASCP), QIHC ( ASCP) Specialists in Dermatology Histology/Mohs Supervisor Tucson, AZ --------------------------------- Boardwalk for $500? In 2007? Ha! Play Monopoly Here and Now (it's updated for today's economy) at Yahoo! Games. From jm.lapointe <@t> accellab.com Thu Sep 13 09:46:27 2007 From: jm.lapointe <@t> accellab.com (Jean-Martin Lapointe) Date: Thu Sep 13 09:48:26 2007 Subject: [Histonet] Azan-Mallory Message-ID: Greetings, My lab has been asked to do Azan-Mallory stains for a study. I cannot find a technique anywhere for this stain - not in the histotech books we have, not on various web sites listing histo techniques, not in the literature. Many literature papers cite the use of this stain as if it was a standard method, without any reference. I am wondering if it could be another name for a commonly used stain. Can anyone help ? _________________________________ Jean-Martin Lapointe, DMV, MS, dACVP AccelLAB Inc From HornHV <@t> archildrens.org Thu Sep 13 09:54:03 2007 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Sep 13 09:54:30 2007 Subject: [Histonet] RE: IHC and State requirements In-Reply-To: <40413.11554.qm@web50603.mail.re2.yahoo.com> References: <40413.11554.qm@web50603.mail.re2.yahoo.com> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB733D@EMAIL.archildrens.org> Being grandfathered in did not mean they just gave you the title of HTL. The grandfathered HTL's had to pass the tests just like anyone else! Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Tournear Sent: Thursday, September 13, 2007 9:27 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: IHC and State requirements Hi Michelle, A lot employers will except either HT or HTL. And of course, how much experience you have under your belt is also a big consideration. I think a lot of employers are finding out just how hard it is to find techs (registered or not) that can do the job and do it well, although there are a few states with their own state requirements which would require the tech to be ASCP certified either way. I have found that being an HTL vs HT doesn't necessarily mean that one or the other can do the job better...I know a few HTL's that were grandfathered in and have done only a handful IHCs in their whole career, can't trouble shoot anything, and produce poor quality slides and yet they hold the title of an HTL and the same goes for the HTs...(makes you wonder how they keep a job)...LOL....and I have taught and worked with students right out of college programs that were absolutely awesome and knew their stuff....go figure.....I say go for it.....Good Luck.... Kim Tournear, HT (ASCP), QIHC ( ASCP) Specialists in Dermatology Histology/Mohs Supervisor Tucson, AZ --------------------------------- Boardwalk for $500? In 2007? Ha! Play Monopoly Here and Now (it's updated for today's economy) at Yahoo! Games. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From kmerriam2003 <@t> yahoo.com Thu Sep 13 09:59:21 2007 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Thu Sep 13 09:59:34 2007 Subject: Fw: [Histonet]immuno stainers Message-ID: <955842.75061.qm@web50304.mail.re2.yahoo.com> Here was my response, I inadvertantly hit the "reply to sender" button instead of "reply to all". Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA ----- Forwarded Message ---- From: Kim Merriam To: Christine Tambasco Sent: Thursday, September 13, 2007 9:23:14 AM Subject: Re: [Histonet]immuno stainers I have been using a Nemesis for about 4 years now and I love the machine. The reason that I bought it was due to its large capacity (up to 84 slides); but I find that it is very flexible in terms of using multiple staining protocols and antibodies on the same run. I work in a biotech research histology lab, so we rarely do the same stain from day to day; so flexibility is key in this environment. The software is very similar to the DAKO machine and the machine itself is the same as the LabVision machine. One thing I have to say is that BIocare is a very customer-oriented company, which is very important to me. If I have a question about a stain (or the machine), they are very available to help me out. Also, if there is a problem with the machine, the repair guy comes right away to fix it. I can only say that about a few companies that I have dealt with. If you have any specific questions, let me know. Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA ----- Original Message ---- From: Christine Tambasco To: histomike@charter.net; histonet@lists.utsouthwestern.edu Sent: Wednesday, September 12, 2007 5:25:55 PM Subject: Re: [Histonet]immuno stainers Anybody have an experiences with the Nemisis from BioCare? Would appreciate any feed back. Thanks Christine Tambasco, HT (ASCP) St Marys Hospital, Amsterdam NY ______________________________________________________________ From: "Mike Schlicht" To: Subject: Re: [Histonet] Special Stainers Date: Wed, 12 Sep 2007 12:51:34 -0700 >We are currently using the DAKO Artisan for silver stains, and have >some problems with uneven staining every now and then, usually when >the dispensers are close to being empty, so we stop using the kit >when there are 5 tests remaining to help reduce some of the >problems. We had a demo of the Nexus stainer, and found that the >result were about the same. >Mike >San Luis Obispo, CA > > >>Message: 15 >>Date: Tue, 11 Sep 2007 18:32:37 -0500 >>From: "Rebecca Johnson" >>Subject: [Histonet] Special Stainers >>To: "histonet" >>Message-ID: <011a01c7f4cc$09fefcc0$7b48f9d8@CHURCH> >>Content-Type: text/plain; charset="iso-8859-1" >> >>What special stainers is everyone using? I now have a Ventana >>Nexus. Bought in 1999. I know this is old, but it has been >>maintained well. We have had problems with it several times over >>the years. Mostly with sliver stains or uneven staining and no >>staining. I would like to know what others are using and your >>experiences are. Thanks for all you help. >>raj >> > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ [1]Kick back and relax with hot games and cool activities at the Messenger Caf?. References 1. http://g.msn.com/8HMBENUS/2740??PS=47575 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. ____________________________________________________________________________________ Catch up on fall's hot new shows on Yahoo! TV. Watch previews, get listings, and more! http://tv.yahoo.com/collections/3658 From sheila_adey <@t> hotmail.com Thu Sep 13 10:06:42 2007 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Thu Sep 13 10:07:03 2007 Subject: [Histonet]immuno stainers In-Reply-To: Message-ID: We also have the Dako Artisan and really like the results. We also don't use right down to the last test because people prime at diffierent rates so it is hard to say exactly how much is left in the kit. Sheila Adey HT MLT Port Huron Hospital Michigan >From: "Sebree Linda A." >To: "Christine Tambasco" , >, >Subject: RE: [Histonet]immuno stainers >Date: Thu, 13 Sep 2007 08:09:40 -0500 > >Please post replies to Histonet. > >Linda Sebree, HT(ASCP) >University of Wisconsin Hospital & Clinics >IHC/ISH Laboratory >A4/204-3224 >600 Highland Ave. >Madison, WI 53792 >(608)265-6596 >FAX: (608)262-7174 > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Christine >Tambasco >Sent: Wednesday, September 12, 2007 4:26 PM >To: histomike@charter.net; histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet]immuno stainers > > > > Anybody have an experiences with the Nemisis from BioCare? Would > appreciate any feed back. Thanks > > Christine Tambasco, HT (ASCP) > > St Marys Hospital, Amsterdam NY > ______________________________________________________________ > > From: "Mike Schlicht" > To: > Subject: Re: [Histonet] Special Stainers > Date: Wed, 12 Sep 2007 12:51:34 -0700 > >We are currently using the DAKO Artisan for silver stains, and > have > >some problems with uneven staining every now and then, usually > when > >the dispensers are close to being empty, so we stop using the kit > >when there are 5 tests remaining to help reduce some of the > >problems. We had a demo of the Nexus stainer, and found that the > >result were about the same. > >Mike > >San Luis Obispo, CA > > > > > >>Message: 15 > >>Date: Tue, 11 Sep 2007 18:32:37 -0500 > >>From: "Rebecca Johnson" > >>Subject: [Histonet] Special Stainers > >>To: "histonet" > >>Message-ID: <011a01c7f4cc$09fefcc0$7b48f9d8@CHURCH> > >>Content-Type: text/plain; charset="iso-8859-1" > >> > >>What special stainers is everyone using? I now have a Ventana > >>Nexus. Bought in 1999. I know this is old, but it has been > >>maintained well. We have had problems with it several times over > >>the years. Mostly with sliver stains or uneven staining and no > >>staining. I would like to know what others are using and your > >>experiences are. Thanks for all you help. > >>raj > >> > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _________________________________________________________________ > > [1]Kick back and relax with hot games and cool activities at the > Messenger Café. > >References > > 1. http://g.msn.com/8HMBENUS/2740??PS=47575 >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Enter to win a night a VIP night out at TIFF http://redcarpet.sympatico.msn.ca/ From bhewlett <@t> cogeco.ca Thu Sep 13 10:14:48 2007 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Thu Sep 13 10:15:02 2007 Subject: [Histonet] Azan-Mallory References: Message-ID: <000b01c7f618$d353f010$6500a8c0@mainbox> Hi Jean-Martin, The reason that you cannot locate the Azan-Mallory stain is because you have been given the wrong name! The stain is actually Heidenhain's 'Azan' modification of Mallory's connective tissue stain (trichrome). A google search for 'Heidenhain's azan' will get you this website with the method. http://stainsfile.info/StainsFile/stain/conektv/tri_heidenhain.htm Regards, Bryan ----- Original Message ----- From: "Jean-Martin Lapointe" To: Sent: Thursday, September 13, 2007 10:46 AM Subject: [Histonet] Azan-Mallory Greetings, My lab has been asked to do Azan-Mallory stains for a study. I cannot find a technique anywhere for this stain - not in the histotech books we have, not on various web sites listing histo techniques, not in the literature. Many literature papers cite the use of this stain as if it was a standard method, without any reference. I am wondering if it could be another name for a commonly used stain. Can anyone help ? _________________________________ Jean-Martin Lapointe, DMV, MS, dACVP AccelLAB Inc _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From m5johnso <@t> meded.ucsd.edu Thu Sep 13 10:21:02 2007 From: m5johnso <@t> meded.ucsd.edu (Mindy Johnson) Date: Thu Sep 13 10:21:23 2007 Subject: [Histonet] Azan-Mallory In-Reply-To: References: Message-ID: <00b801c7f619$b36dc7c0$1a495740$@ucsd.edu> I also have a procedure in my Animal Tissue Techniques 5th Edition book. Under the Heidenhain Azan Stain. ?Mindy A Johnson SRA II UCSD - School of Medicine Medical Teaching Labs -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jean-Martin Lapointe Sent: Thursday, September 13, 2007 7:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Azan-Mallory Greetings, My lab has been asked to do Azan-Mallory stains for a study. I cannot find a technique anywhere for this stain - not in the histotech books we have, not on various web sites listing histo techniques, not in the literature. Many literature papers cite the use of this stain as if it was a standard method, without any reference. I am wondering if it could be another name for a commonly used stain. Can anyone help ? _________________________________ Jean-Martin Lapointe, DMV, MS, dACVP AccelLAB Inc _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Sep 13 10:23:45 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 13 10:23:58 2007 Subject: [Histonet] Histonet at NSH?? In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B8F4689@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <436951.24201.qm@web61222.mail.yahoo.com> Sara: You will be older than me, only if you are older than 73! Ren? J. "Breeden, Sara" wrote: Will there be a Histonet Gathering at NSH in Denver this year? Or would someone like to plan one? I have recently resigned from my position as The Pearl (Perle?) Mesta of That alone ought to tell you that I'm at least as old as Rene (just joshin', Rene...). Maybe a gathering is not a good idea if I keep insulting perfect strangers, huh? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Luggage? GPS? Comic books? Check out fitting gifts for grads at Yahoo! Search. From kimtournear <@t> yahoo.com Thu Sep 13 10:27:04 2007 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Thu Sep 13 10:27:17 2007 Subject: [Histonet] RE: IHC and State requirements In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB733D@EMAIL.archildrens.org> Message-ID: <205826.36402.qm@web50601.mail.re2.yahoo.com> To all you HTLs out there... Please except my apology if my response to another email was offensive...What I meant was HTL vs HT doesn't mean one or the other can do the job better than the other just because of the title....and I do realize that the test has to be passed in order to obtain the title regardless of the route of entry...If I offended anyone out there, please except my apology...Sorry.... "Horn, Hazel V" wrote: Being grandfathered in did not mean they just gave you the title of HTL. The grandfathered HTL's had to pass the tests just like anyone else! Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Tournear Sent: Thursday, September 13, 2007 9:27 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: IHC and State requirements Hi Michelle, A lot employers will except either HT or HTL. And of course, how much experience you have under your belt is also a big consideration. I think a lot of employers are finding out just how hard it is to find techs (registered or not) that can do the job and do it well, although there are a few states with their own state requirements which would require the tech to be ASCP certified either way. I have found that being an HTL vs HT doesn't necessarily mean that one or the other can do the job better...I know a few HTL's that were grandfathered in and have done only a handful IHCs in their whole career, can't trouble shoot anything, and produce poor quality slides and yet they hold the title of an HTL and the same goes for the HTs...(makes you wonder how they keep a job)...LOL....and I have taught and worked with students right out of college programs that were absolutely awesome and knew their stuff....go figure.....I say go for it.....Good Luck.... Kim Tournear, HT (ASCP), QIHC ( ASCP) Specialists in Dermatology Histology/Mohs Supervisor Tucson, AZ --------------------------------- Boardwalk for $500? In 2007? Ha! Play Monopoly Here and Now (it's updated for today's economy) at Yahoo! Games. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== Kim Tournear, HT (ASCP), QIHC ( ASCP) Specialists in Dermatology Histology/Mohs Supervisor Tucson, AZ --------------------------------- Shape Yahoo! in your own image. Join our Network Research Panel today! From rjbuesa <@t> yahoo.com Thu Sep 13 10:29:43 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 13 10:29:54 2007 Subject: [Histonet] IHC and state requirements In-Reply-To: <25355ef80709121430v7a719e0cvb28fd694c0341611@mail.gmail.com> Message-ID: <128056.38013.qm@web61217.mail.yahoo.com> Generally speaking, Histotechnologists (HTL) is a work position reserved to those performing "high complexity tests", and histotechnician (HT) does not imply them. IHC is considered a "high complexity test, as DIF, INDIF, ISH, FISH, and TEM. Ren? J. Michelle McCoy wrote: I am a Florida licensed HT (ASCP). I'm planning to apply for work out of the state and have seen jobs titled "Histotechnologist" and was curious if I am eligible to apply for jobs with this title --if this term is only applied for HTL credential holders. Or if it is applied to anyone who has histotechnology experience (I believe someone had mentioned you dont have to have HT or HTL credentials to work in the field in many states). Some job listings state IHC duties and I'm guessing I can perform them as an HT (ASCP) if I have experience or they are willing to train in IHC (unless otherwise indicated that they are only looking for HTL candidates) Thanks for any clarification on this. Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Pinpoint customers who are looking for what you sell. From b-frederick <@t> northwestern.edu Thu Sep 13 10:33:28 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Sep 13 10:33:53 2007 Subject: [Histonet] RE: IHC and State requirements In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB733D@EMAIL.archildrens.org> Message-ID: <000901c7f61b$71c62770$d00f7ca5@lurie.northwestern.edu> Hazel, I agree with you- I know people who are grandfathered and took the exam. I've met one person who helped create the HTL certification requirements. I'm just an HTL but do agree that some have the HTL but only know basic histo. I know of some institutions where an HTL is preferred when it comes to IHC (based on the BS). My pet peeve is post-docs that think they know everything but can't comprehend histo and thinek they can do microtomy. We require certification for those people that are cutting blocks that are human cancer trial specimens (a long story) Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, September 13, 2007 9:54 AM To: Kim Tournear; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: IHC and State requirements Being grandfathered in did not mean they just gave you the title of HTL. The grandfathered HTL's had to pass the tests just like anyone else! Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Tournear Sent: Thursday, September 13, 2007 9:27 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: IHC and State requirements Hi Michelle, A lot employers will except either HT or HTL. And of course, how much experience you have under your belt is also a big consideration. I think a lot of employers are finding out just how hard it is to find techs (registered or not) that can do the job and do it well, although there are a few states with their own state requirements which would require the tech to be ASCP certified either way. I have found that being an HTL vs HT doesn't necessarily mean that one or the other can do the job better...I know a few HTL's that were grandfathered in and have done only a handful IHCs in their whole career, can't trouble shoot anything, and produce poor quality slides and yet they hold the title of an HTL and the same goes for the HTs...(makes you wonder how they keep a job)...LOL....and I have taught and worked with students right out of college programs that were absolutely awesome and knew their stuff....go figure.....I say go for it.....Good Luck.... Kim Tournear, HT (ASCP), QIHC ( ASCP) Specialists in Dermatology Histology/Mohs Supervisor Tucson, AZ --------------------------------- Boardwalk for $500? In 2007? Ha! Play Monopoly Here and Now (it's updated for today's economy) at Yahoo! Games. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------------- -- The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================ == _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Thu Sep 13 10:40:47 2007 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Thu Sep 13 10:41:06 2007 Subject: [Histonet] IHC and state requirements Message-ID: <57BE698966D5C54EAE8612E8941D768301268F28@EXCHANGE3.huntingtonhospital.com> I am asking this question for someone from another lab. They recently got a new lab manager who stated that the person doing IHC's, who is not licensed as either an HT or HTL but is a Ph.D, is not qualified to be performing IHC's because it involves the dilution of antibodies. Evidently, she is comparing this "requirement" which is applied to Med Techs to that of HT's. So, I know in the state of California (where we are), there is no state licensure required for HT's, but is there any requirement that applies specifically for the dilution of antibodies - in California or elsewhere??? Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Michelle McCoy Sent: Wednesday, September 12, 2007 2:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC and state requirements I am a Florida licensed HT (ASCP). I'm planning to apply for work out of the state and have seen jobs titled "Histotechnologist" and was curious if I am eligible to apply for jobs with this title --if this term is only applied for HTL credential holders. Or if it is applied to anyone who has histotechnology experience (I believe someone had mentioned you dont have to have HT or HTL credentials to work in the field in many states). Some job listings state IHC duties and I'm guessing I can perform them as an HT (ASCP) if I have experience or they are willing to train in IHC (unless otherwise indicated that they are only looking for HTL candidates) Thanks for any clarification on this. Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alaskagirl1950 <@t> yahoo.com Thu Sep 13 10:46:18 2007 From: alaskagirl1950 <@t> yahoo.com (Patricia Adams) Date: Thu Sep 13 10:46:30 2007 Subject: [Histonet] RE: IHC and State requirements In-Reply-To: <000901c7f61b$71c62770$d00f7ca5@lurie.northwestern.edu> Message-ID: <390188.52970.qm@web52506.mail.re2.yahoo.com> I am a HT, but have been doing IHC since it was manual, doing up to 15 different antibodies at a time and kept it all straight in my head. I am not sure that having an HLT would have helped with that. So where do some of us "older" techs fit into the scheme of things. How do we let the people hiring know that even though we do not have the title, we know what we are doing? Patricia --- Bernice Frederick wrote: > Hazel, > I agree with you- I know people who are > grandfathered and took the exam. > I've met one person who helped create the HTL > certification requirements. > I'm just an HTL but do agree that some have the > HTL but only know basic > histo. I know of some institutions where an HTL > is preferred when it comes > to IHC (based on the BS). My pet peeve is > post-docs that think they know > everything but can't comprehend histo and > thinek they can do microtomy. We > require certification for those people that are > cutting blocks that are > human cancer trial specimens (a long story) > Bernice > > Bernice Frederick HTL (ASCP) > Northwestern University > Pathology Core Facility > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Horn, Hazel > V > Sent: Thursday, September 13, 2007 9:54 AM > To: Kim Tournear; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: IHC and State > requirements > > Being grandfathered in did not mean they just > gave you the title of HTL. > The grandfathered HTL's had to pass the tests > just like anyone else! > > Hazel Horn > Hazel Horn, HT/HTL (ASCP) > Supervisor of Histology > Arkansas Children's Hospital > 800 Marshall Slot 820 > Little Rock, AR 72202 > > phone 501.364.4240 > fax 501.364.3912 > > visit us on the web at: www.archildrens.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Kim > Tournear > Sent: Thursday, September 13, 2007 9:27 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: IHC and State > requirements > > Hi Michelle, > A lot employers will except either HT or HTL. > And of course, how much > experience you have under your belt is also a > big consideration. I > think a lot of employers are finding out just > how hard it is to find > techs (registered or not) that can do the job > and do it well, although > there are a few states with their own state > requirements which would > require the tech to be ASCP certified either > way. > > I have found that being an HTL vs HT doesn't > necessarily mean that one > or the other can do the job better...I know a > few HTL's that were > grandfathered in and have done only a handful > IHCs in their whole > career, can't trouble shoot anything, and > produce poor quality slides > and yet they hold the title of an HTL and the > same goes for the > HTs...(makes you wonder how they keep a > job)...LOL....and I have taught > and worked with students right out of college > programs that were > absolutely awesome and knew their stuff....go > figure.....I say go for > it.....Good Luck.... > > > Kim Tournear, HT (ASCP), QIHC ( ASCP) > Specialists in Dermatology > Histology/Mohs Supervisor > Tucson, AZ > > > > > --------------------------------- > Boardwalk for $500? In 2007? Ha! > Play Monopoly Here and Now (it's updated for > today's economy) at Yahoo! > Games. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ---------------------------------------------------------------------------- > -- > The information contained in this message may > be privileged and confidential > and protected from disclosure. If the reader of > this message is not the > intended recipient, or an employee or agent > responsible for delivering this > message to the intended recipient, you are > hereby notified that any > dissemination, distribution or copying of this > communication is strictly > prohibited. If you have received this > communication in error, please notify > us immediately by replying to the message and > deleting it from your > computer. > Thank you. > ============================================================================ > == > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D ____________________________________________________________________________________ Yahoo! oneSearch: Finally, mobile search that gives answers, not web links. http://mobile.yahoo.com/mobileweb/onesearch?refer=1ONXIC From rjbuesa <@t> yahoo.com Thu Sep 13 10:48:34 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 13 10:48:46 2007 Subject: [Histonet] IHC and state requirements In-Reply-To: <57BE698966D5C54EAE8612E8941D768301268F28@EXCHANGE3.huntingtonhospital.com> Message-ID: <62303.82639.qm@web61225.mail.yahoo.com> Probably that lab manager does not know what a dilution is. Anybody that has studied basic physics, or chemistry know what a dilution rate is. Unless the person you are referring to is a PhD on "the ocult" or on arts or the religious field, s/he will know what a dilution is. Ren? J. Laurie Colbert wrote: I am asking this question for someone from another lab. They recently got a new lab manager who stated that the person doing IHC's, who is not licensed as either an HT or HTL but is a Ph.D, is not qualified to be performing IHC's because it involves the dilution of antibodies. Evidently, she is comparing this "requirement" which is applied to Med Techs to that of HT's. So, I know in the state of California (where we are), there is no state licensure required for HT's, but is there any requirement that applies specifically for the dilution of antibodies - in California or elsewhere??? Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Michelle McCoy Sent: Wednesday, September 12, 2007 2:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC and state requirements I am a Florida licensed HT (ASCP). I'm planning to apply for work out of the state and have seen jobs titled "Histotechnologist" and was curious if I am eligible to apply for jobs with this title --if this term is only applied for HTL credential holders. Or if it is applied to anyone who has histotechnology experience (I believe someone had mentioned you dont have to have HT or HTL credentials to work in the field in many states). Some job listings state IHC duties and I'm guessing I can perform them as an HT (ASCP) if I have experience or they are willing to train in IHC (unless otherwise indicated that they are only looking for HTL candidates) Thanks for any clarification on this. Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Shape Yahoo! in your own image. Join our Network Research Panel today! From rjbuesa <@t> yahoo.com Thu Sep 13 10:56:54 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 13 10:57:07 2007 Subject: [Histonet] RE: IHC and State requirements In-Reply-To: <390188.52970.qm@web52506.mail.re2.yahoo.com> Message-ID: <295397.47064.qm@web61217.mail.yahoo.com> That is exactly my contention in an article I just finished about wages in histology. When you apply to a job, or negotiate your salary, you are not applying as part of a group or class, you are negotiating your personal salary and your experience and abilities are personal, particular, independent of that of others, with or without a title. From all the people I have supervised I have had the best amongst those with lower academic levels, and, viceversa! The title and the studies qualify you for a title, but does not habilitate you for the tasks! Ren? J. Patricia Adams wrote: I am a HT, but have been doing IHC since it was manual, doing up to 15 different antibodies at a time and kept it all straight in my head. I am not sure that having an HLT would have helped with that. So where do some of us "older" techs fit into the scheme of things. How do we let the people hiring know that even though we do not have the title, we know what we are doing? Patricia --- Bernice Frederick wrote: > Hazel, > I agree with you- I know people who are > grandfathered and took the exam. > I've met one person who helped create the HTL > certification requirements. > I'm just an HTL but do agree that some have the > HTL but only know basic > histo. I know of some institutions where an HTL > is preferred when it comes > to IHC (based on the BS). My pet peeve is > post-docs that think they know > everything but can't comprehend histo and > thinek they can do microtomy. We > require certification for those people that are > cutting blocks that are > human cancer trial specimens (a long story) > Bernice > > Bernice Frederick HTL (ASCP) > Northwestern University > Pathology Core Facility > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Horn, Hazel > V > Sent: Thursday, September 13, 2007 9:54 AM > To: Kim Tournear; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: IHC and State > requirements > > Being grandfathered in did not mean they just > gave you the title of HTL. > The grandfathered HTL's had to pass the tests > just like anyone else! > > Hazel Horn > Hazel Horn, HT/HTL (ASCP) > Supervisor of Histology > Arkansas Children's Hospital > 800 Marshall Slot 820 > Little Rock, AR 72202 > > phone 501.364.4240 > fax 501.364.3912 > > visit us on the web at: www.archildrens.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Kim > Tournear > Sent: Thursday, September 13, 2007 9:27 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: IHC and State > requirements > > Hi Michelle, > A lot employers will except either HT or HTL. > And of course, how much > experience you have under your belt is also a > big consideration. I > think a lot of employers are finding out just > how hard it is to find > techs (registered or not) that can do the job > and do it well, although > there are a few states with their own state > requirements which would > require the tech to be ASCP certified either > way. > > I have found that being an HTL vs HT doesn't > necessarily mean that one > or the other can do the job better...I know a > few HTL's that were > grandfathered in and have done only a handful > IHCs in their whole > career, can't trouble shoot anything, and > produce poor quality slides > and yet they hold the title of an HTL and the > same goes for the > HTs...(makes you wonder how they keep a > job)...LOL....and I have taught > and worked with students right out of college > programs that were > absolutely awesome and knew their stuff....go > figure.....I say go for > it.....Good Luck.... > > > Kim Tournear, HT (ASCP), QIHC ( ASCP) > Specialists in Dermatology > Histology/Mohs Supervisor > Tucson, AZ > > > > > --------------------------------- > Boardwalk for $500? In 2007? Ha! > Play Monopoly Here and Now (it's updated for > today's economy) at Yahoo! > Games. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ---------------------------------------------------------------------------- > -- > The information contained in this message may > be privileged and confidential > and protected from disclosure. If the reader of > this message is not the > intended recipient, or an employee or agent > responsible for delivering this > message to the intended recipient, you are > hereby notified that any > dissemination, distribution or copying of this > communication is strictly > prohibited. If you have received this > communication in error, please notify > us immediately by replying to the message and > deleting it from your > computer. > Thank you. > ============================================================================ > == > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D ____________________________________________________________________________________ Yahoo! oneSearch: Finally, mobile search that gives answers, not web links. http://mobile.yahoo.com/mobileweb/onesearch?refer=1ONXIC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better Heartthrob. Get better relationship answers from someone who knows. Yahoo! Answers - Check it out. From simmonsca <@t> upmc.edu Thu Sep 13 11:00:00 2007 From: simmonsca <@t> upmc.edu (Simmons, Christopher) Date: Thu Sep 13 11:00:19 2007 Subject: [Histonet] RE: IHC and State requirements In-Reply-To: <390188.52970.qm@web52506.mail.re2.yahoo.com> References: <000901c7f61b$71c62770$d00f7ca5@lurie.northwestern.edu> <390188.52970.qm@web52506.mail.re2.yahoo.com> Message-ID: <88D6F512ADC2CB428B90845D2D1388E303A5BB5D@1upmc-msx6.acct.upmchs.net> The only real difference besides the complexity of tests being done, is the education. 4 year degree..HTL..2 year...HT. That is the only difference I make of the whole thing. I utilize my staff according to their abilities, not by their qualifications. Chris Simmons Lead Tech PUH Histology 412.647.7660 desk 13242 pager -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patricia Adams Sent: Thursday, September 13, 2007 11:46 AM To: Bernice Frederick; HistoNet Subject: RE: [Histonet] RE: IHC and State requirements I am a HT, but have been doing IHC since it was manual, doing up to 15 different antibodies at a time and kept it all straight in my head. I am not sure that having an HLT would have helped with that. So where do some of us "older" techs fit into the scheme of things. How do we let the people hiring know that even though we do not have the title, we know what we are doing? Patricia --- Bernice Frederick wrote: > Hazel, > I agree with you- I know people who are grandfathered and took the > exam. > I've met one person who helped create the HTL certification > requirements. > I'm just an HTL but do agree that some have the HTL but only know > basic histo. I know of some institutions where an HTL is preferred > when it comes to IHC (based on the BS). My pet peeve is post-docs that > think they know everything but can't comprehend histo and thinek they > can do microtomy. We require certification for those people that are > cutting blocks that are human cancer trial specimens (a long story) > Bernice > > Bernice Frederick HTL (ASCP) > Northwestern University > Pathology Core Facility > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Horn, Hazel > V > Sent: Thursday, September 13, 2007 9:54 AM > To: Kim Tournear; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: IHC and State requirements > > Being grandfathered in did not mean they just gave you the title of > HTL. > The grandfathered HTL's had to pass the tests just like anyone else! > > Hazel Horn > Hazel Horn, HT/HTL (ASCP) > Supervisor of Histology > Arkansas Children's Hospital > 800 Marshall Slot 820 > Little Rock, AR 72202 > > phone 501.364.4240 > fax 501.364.3912 > > visit us on the web at: www.archildrens.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Kim > Tournear > Sent: Thursday, September 13, 2007 9:27 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: IHC and State > requirements > > Hi Michelle, > A lot employers will except either HT or HTL. > And of course, how much > experience you have under your belt is also a big consideration. I > think a lot of employers are finding out just how hard it is to find > techs (registered or not) that can do the job and do it well, although > there are a few states with their own state requirements which would > require the tech to be ASCP certified either way. > > I have found that being an HTL vs HT doesn't necessarily mean that > one or the other can do the job better...I know a few HTL's that were > grandfathered in and have done only a handful IHCs in their whole > career, can't trouble shoot anything, and produce poor quality slides > and yet they hold the title of an HTL and the same goes for the > HTs...(makes you wonder how they keep a job)...LOL....and I have > taught and worked with students right out of college programs that > were absolutely awesome and knew their stuff....go figure.....I say go > for it.....Good Luck.... > > > Kim Tournear, HT (ASCP), QIHC ( ASCP) > Specialists in Dermatology > Histology/Mohs Supervisor > Tucson, AZ > > > > > --------------------------------- > Boardwalk for $500? In 2007? Ha! > Play Monopoly Here and Now (it's updated for today's economy) at > Yahoo! > Games. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------------------------------------------------ ---- > -- > The information contained in this message may be privileged and > confidential and protected from disclosure. If the reader of this > message is not the intended recipient, or an employee or agent > responsible for delivering this message to the intended recipient, you > are hereby notified that any dissemination, distribution or copying of > this communication is strictly prohibited. If you have received this > communication in error, please notify us immediately by replying to > the message and deleting it from your computer. > Thank you. > ======================================================================== ==== > == > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D ________________________________________________________________________ ____________ Yahoo! oneSearch: Finally, mobile search that gives answers, not web links. http://mobile.yahoo.com/mobileweb/onesearch?refer=1ONXIC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Thu Sep 13 11:11:54 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Sep 13 11:13:31 2007 Subject: [Histonet] IHC and state requirements References: <57BE698966D5C54EAE8612E8941D768301268F28@EXCHANGE3.huntingtonhospital.com> Message-ID: <001c01c7f620$fdd835a0$0202a8c0@yourxhtr8hvc4p> huh? not in Texas. My immuno tech is an HT and she performs serial dilutions. JTT ----- Original Message ----- From: "Laurie Colbert" To: "Michelle McCoy" ; Cc: Sent: Thursday, September 13, 2007 10:40 AM Subject: RE: [Histonet] IHC and state requirements I am asking this question for someone from another lab. They recently got a new lab manager who stated that the person doing IHC's, who is not licensed as either an HT or HTL but is a Ph.D, is not qualified to be performing IHC's because it involves the dilution of antibodies. Evidently, she is comparing this "requirement" which is applied to Med Techs to that of HT's. So, I know in the state of California (where we are), there is no state licensure required for HT's, but is there any requirement that applies specifically for the dilution of antibodies - in California or elsewhere??? Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Michelle McCoy Sent: Wednesday, September 12, 2007 2:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC and state requirements I am a Florida licensed HT (ASCP). I'm planning to apply for work out of the state and have seen jobs titled "Histotechnologist" and was curious if I am eligible to apply for jobs with this title --if this term is only applied for HTL credential holders. Or if it is applied to anyone who has histotechnology experience (I believe someone had mentioned you dont have to have HT or HTL credentials to work in the field in many states). Some job listings state IHC duties and I'm guessing I can perform them as an HT (ASCP) if I have experience or they are willing to train in IHC (unless otherwise indicated that they are only looking for HTL candidates) Thanks for any clarification on this. Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Thu Sep 13 11:12:51 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Sep 13 11:13:35 2007 Subject: [Histonet] IHC and state requirements References: <62303.82639.qm@web61225.mail.yahoo.com> Message-ID: <001d01c7f620$ffc5adc0$0202a8c0@yourxhtr8hvc4p> that's scary. A lab manager who doesn't know what a dilution is. JTT ----- Original Message ----- From: "Rene J Buesa" To: "Laurie Colbert" ; "Michelle McCoy" ; Cc: Sent: Thursday, September 13, 2007 10:48 AM Subject: RE: [Histonet] IHC and state requirements > Probably that lab manager does not know what a dilution is. Anybody that > has studied basic physics, or chemistry know what a dilution rate is. > Unless the person you are referring to is a PhD on "the ocult" or on arts > or the religious field, s/he will know what a dilution is. > Ren? J. > > Laurie Colbert wrote: > I am asking this question for someone from another lab. They recently got > a new lab manager who stated that the person doing IHC's, who is not > licensed as either an HT or HTL but is a Ph.D, is not qualified to be > performing IHC's because it involves the dilution of antibodies. > Evidently, she is comparing this "requirement" which is applied to Med > Techs to that of HT's. > So, I know in the state of California (where we are), there is no state > licensure required for HT's, but is there any requirement that applies > specifically for the dilution of antibodies - in California or > elsewhere??? > > Laurie Colbert > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Michelle > McCoy > Sent: Wednesday, September 12, 2007 2:31 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] IHC and state requirements > > > I am a Florida licensed HT (ASCP). I'm planning to apply for work out of > the > state and have seen jobs titled "Histotechnologist" and was curious if I > am > eligible to apply for jobs with this title --if this term is only applied > for HTL credential holders. Or if it is applied to anyone who has > histotechnology experience (I believe someone had mentioned you dont have > to > have HT or HTL credentials to work in the field in many states). Some job > listings state IHC duties and I'm guessing I can perform them as an HT > (ASCP) if I have experience or they are willing to train in IHC (unless > otherwise indicated that they are only looking for HTL candidates) Thanks > for any clarification on this. > > Michelle > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Shape Yahoo! in your own image. Join our Network Research Panel today! > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Vickroy.Jim <@t> mhsil.com Thu Sep 13 11:14:59 2007 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Thu Sep 13 11:16:34 2007 Subject: [Histonet] Histotech vacancies Message-ID: <24A4826E8EF0964D86BC5317306F58A502FEBB8586@mmc-mail.ad.mhsil.com> Like a lot of hospitals we have been unable to find qualified applicants for a histotech position. We are at least exploring other options. One of the options include hiring someone with a biological background and enrolling them in an on-line histotech training program. After completion of the program the applicant would take the certification examination. There are a few schools out there that have full time programs but many individuals are not in a position to take six months off to attend them without receiving a weekly paycheck. In addition my experience has been that these schools only take a small amount of applicants each session. So with more positions than students has anyone else come up with a creative way to train and hire histotechs and any suggestions to on-line programs that have been worthwhile. Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From jnocito <@t> satx.rr.com Thu Sep 13 11:16:36 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Sep 13 11:16:49 2007 Subject: [Histonet] RE: IHC and State requirements References: <295397.47064.qm@web61217.mail.yahoo.com> Message-ID: <002e01c7f621$75ab1b10$0202a8c0@yourxhtr8hvc4p> to add to Rene's statement. My degrees, titles and experiences got my foot in the door, but it was my experiences that kept me there. JTT ----- Original Message ----- From: "Rene J Buesa" To: "Patricia Adams" ; "Bernice Frederick" ; "HistoNet" Sent: Thursday, September 13, 2007 10:56 AM Subject: RE: [Histonet] RE: IHC and State requirements > That is exactly my contention in an article I just finished about wages in > histology. > When you apply to a job, or negotiate your salary, you are not applying > as part of a group or class, you are negotiating your personal salary and > your experience and abilities are personal, particular, independent of > that of others, with or without a title. > From all the people I have supervised I have had the best amongst those > with lower academic levels, and, viceversa! > The title and the studies qualify you for a title, but does not > habilitate you for the tasks! > Ren? J. > > Patricia Adams wrote: > I am a HT, but have been doing IHC since it was > manual, doing up to 15 different antibodies at a > time and kept it all straight in my head. I am > not sure that having an HLT would have helped > with that. So where do some of us "older" techs > fit into the scheme of things. How do we let the > people hiring know that even though we do not > have the title, we know what we are doing? > Patricia > --- Bernice Frederick > wrote: > >> Hazel, >> I agree with you- I know people who are >> grandfathered and took the exam. >> I've met one person who helped create the HTL >> certification requirements. >> I'm just an HTL but do agree that some have the >> HTL but only know basic >> histo. I know of some institutions where an HTL >> is preferred when it comes >> to IHC (based on the BS). My pet peeve is >> post-docs that think they know >> everything but can't comprehend histo and >> thinek they can do microtomy. We >> require certification for those people that are >> cutting blocks that are >> human cancer trial specimens (a long story) >> Bernice >> >> Bernice Frederick HTL (ASCP) >> Northwestern University >> Pathology Core Facility >> 710 N Fairbanks Court >> Olson 8-421 >> Chicago,IL 60611 >> 312-503-3723 >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> > [mailto:histonet-bounces@lists.utsouthwestern.edu] >> On Behalf Of Horn, Hazel >> V >> Sent: Thursday, September 13, 2007 9:54 AM >> To: Kim Tournear; >> histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] RE: IHC and State >> requirements >> >> Being grandfathered in did not mean they just >> gave you the title of HTL. >> The grandfathered HTL's had to pass the tests >> just like anyone else! >> >> Hazel Horn >> Hazel Horn, HT/HTL (ASCP) >> Supervisor of Histology >> Arkansas Children's Hospital >> 800 Marshall Slot 820 >> Little Rock, AR 72202 >> >> phone 501.364.4240 >> fax 501.364.3912 >> >> visit us on the web at: www.archildrens.org >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> > [mailto:histonet-bounces@lists.utsouthwestern.edu] >> On Behalf Of Kim >> Tournear >> Sent: Thursday, September 13, 2007 9:27 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] RE: IHC and State >> requirements >> >> Hi Michelle, >> A lot employers will except either HT or HTL. >> And of course, how much >> experience you have under your belt is also a >> big consideration. I >> think a lot of employers are finding out just >> how hard it is to find >> techs (registered or not) that can do the job >> and do it well, although >> there are a few states with their own state >> requirements which would >> require the tech to be ASCP certified either >> way. >> >> I have found that being an HTL vs HT doesn't >> necessarily mean that one >> or the other can do the job better...I know a >> few HTL's that were >> grandfathered in and have done only a handful >> IHCs in their whole >> career, can't trouble shoot anything, and >> produce poor quality slides >> and yet they hold the title of an HTL and the >> same goes for the >> HTs...(makes you wonder how they keep a >> job)...LOL....and I have taught >> and worked with students right out of college >> programs that were >> absolutely awesome and knew their stuff....go >> figure.....I say go for >> it.....Good Luck.... >> >> >> Kim Tournear, HT (ASCP), QIHC ( ASCP) >> Specialists in Dermatology >> Histology/Mohs Supervisor >> Tucson, AZ >> >> >> >> >> --------------------------------- >> Boardwalk for $500? In 2007? Ha! >> Play Monopoly Here and Now (it's updated for >> today's economy) at Yahoo! >> Games. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > ---------------------------------------------------------------------------- >> -- >> The information contained in this message may >> be privileged and confidential >> and protected from disclosure. If the reader of >> this message is not the >> intended recipient, or an employee or agent >> responsible for delivering this >> message to the intended recipient, you are >> hereby notified that any >> dissemination, distribution or copying of this >> communication is strictly >> prohibited. If you have received this >> communication in error, please notify >> us immediately by replying to the message and >> deleting it from your >> computer. >> Thank you. >> > ============================================================================ >> == >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > Patricia Adams > ----- > Fight back spam! Download the Blue Frog. > http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D > > > > ____________________________________________________________________________________ > Yahoo! oneSearch: Finally, mobile search > that gives answers, not web links. > http://mobile.yahoo.com/mobileweb/onesearch?refer=1ONXIC > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Be a better Heartthrob. Get better relationship answers from someone who > knows. > Yahoo! Answers - Check it out. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Thu Sep 13 11:20:13 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Sep 13 11:20:30 2007 Subject: [Histonet]immuno stainers References: <955842.75061.qm@web50304.mail.re2.yahoo.com> Message-ID: <003801c7f621$f956d6c0$0202a8c0@yourxhtr8hvc4p> Kim, I had the distinct pleasure (?) of working with other machines, but used Biocare's antibodies and you are right. When we had a problem working up an antibody or a problem with something else, they were always kind, courteous and very patient. Unlike other companies were it took them 3-4 days to fix their machine. Only because they were fixing other machines. (what's that say about the machine)? JTT ----- Original Message ----- From: "Kim Merriam" To: "Histonet" Sent: Thursday, September 13, 2007 9:59 AM Subject: Fw: [Histonet]immuno stainers Here was my response, I inadvertantly hit the "reply to sender" button instead of "reply to all". Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA ----- Forwarded Message ---- From: Kim Merriam To: Christine Tambasco Sent: Thursday, September 13, 2007 9:23:14 AM Subject: Re: [Histonet]immuno stainers I have been using a Nemesis for about 4 years now and I love the machine. The reason that I bought it was due to its large capacity (up to 84 slides); but I find that it is very flexible in terms of using multiple staining protocols and antibodies on the same run. I work in a biotech research histology lab, so we rarely do the same stain from day to day; so flexibility is key in this environment. The software is very similar to the DAKO machine and the machine itself is the same as the LabVision machine. One thing I have to say is that BIocare is a very customer-oriented company, which is very important to me. If I have a question about a stain (or the machine), they are very available to help me out. Also, if there is a problem with the machine, the repair guy comes right away to fix it. I can only say that about a few companies that I have dealt with. If you have any specific questions, let me know. Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA ----- Original Message ---- From: Christine Tambasco To: histomike@charter.net; histonet@lists.utsouthwestern.edu Sent: Wednesday, September 12, 2007 5:25:55 PM Subject: Re: [Histonet]immuno stainers Anybody have an experiences with the Nemisis from BioCare? Would appreciate any feed back. Thanks Christine Tambasco, HT (ASCP) St Marys Hospital, Amsterdam NY ______________________________________________________________ From: "Mike Schlicht" To: Subject: Re: [Histonet] Special Stainers Date: Wed, 12 Sep 2007 12:51:34 -0700 >We are currently using the DAKO Artisan for silver stains, and have >some problems with uneven staining every now and then, usually when >the dispensers are close to being empty, so we stop using the kit >when there are 5 tests remaining to help reduce some of the >problems. We had a demo of the Nexus stainer, and found that the >result were about the same. >Mike >San Luis Obispo, CA > > >>Message: 15 >>Date: Tue, 11 Sep 2007 18:32:37 -0500 >>From: "Rebecca Johnson" >>Subject: [Histonet] Special Stainers >>To: "histonet" >>Message-ID: <011a01c7f4cc$09fefcc0$7b48f9d8@CHURCH> >>Content-Type: text/plain; charset="iso-8859-1" >> >>What special stainers is everyone using? I now have a Ventana >>Nexus. Bought in 1999. I know this is old, but it has been >>maintained well. We have had problems with it several times over >>the years. Mostly with sliver stains or uneven staining and no >>staining. I would like to know what others are using and your >>experiences are. Thanks for all you help. >>raj >> > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ [1]Kick back and relax with hot games and cool activities at the Messenger Caf?. References 1. http://g.msn.com/8HMBENUS/2740??PS=47575 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. ____________________________________________________________________________________ Catch up on fall's hot new shows on Yahoo! TV. Watch previews, get listings, and more! http://tv.yahoo.com/collections/3658 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Thu Sep 13 11:34:15 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Sep 13 11:34:28 2007 Subject: [Histonet] RE: IHC and State requirements In-Reply-To: <295397.47064.qm@web61217.mail.yahoo.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C71D@IS-E2K3.grhs.net> Well put Rene! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, September 13, 2007 10:57 AM To: Patricia Adams; Bernice Frederick; HistoNet Subject: RE: [Histonet] RE: IHC and State requirements That is exactly my contention in an article I just finished about wages in histology. When you apply to a job, or negotiate your salary, you are not applying as part of a group or class, you are negotiating your personal salary and your experience and abilities are personal, particular, independent of that of others, with or without a title. From all the people I have supervised I have had the best amongst those with lower academic levels, and, viceversa! The title and the studies qualify you for a title, but does not habilitate you for the tasks! Ren? J. Patricia Adams wrote: I am a HT, but have been doing IHC since it was manual, doing up to 15 different antibodies at a time and kept it all straight in my head. I am not sure that having an HLT would have helped with that. So where do some of us "older" techs fit into the scheme of things. How do we let the people hiring know that even though we do not have the title, we know what we are doing? Patricia --- Bernice Frederick wrote: > Hazel, > I agree with you- I know people who are > grandfathered and took the exam. > I've met one person who helped create the HTL > certification requirements. > I'm just an HTL but do agree that some have the > HTL but only know basic > histo. I know of some institutions where an HTL > is preferred when it comes > to IHC (based on the BS). My pet peeve is > post-docs that think they know > everything but can't comprehend histo and > thinek they can do microtomy. We > require certification for those people that are > cutting blocks that are > human cancer trial specimens (a long story) > Bernice > > Bernice Frederick HTL (ASCP) > Northwestern University > Pathology Core Facility > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Horn, Hazel > V > Sent: Thursday, September 13, 2007 9:54 AM > To: Kim Tournear; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: IHC and State > requirements > > Being grandfathered in did not mean they just > gave you the title of HTL. > The grandfathered HTL's had to pass the tests > just like anyone else! > > Hazel Horn > Hazel Horn, HT/HTL (ASCP) > Supervisor of Histology > Arkansas Children's Hospital > 800 Marshall Slot 820 > Little Rock, AR 72202 > > phone 501.364.4240 > fax 501.364.3912 > > visit us on the web at: www.archildrens.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Kim > Tournear > Sent: Thursday, September 13, 2007 9:27 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: IHC and State > requirements > > Hi Michelle, > A lot employers will except either HT or HTL. > And of course, how much > experience you have under your belt is also a > big consideration. I > think a lot of employers are finding out just > how hard it is to find > techs (registered or not) that can do the job > and do it well, although > there are a few states with their own state > requirements which would > require the tech to be ASCP certified either > way. > > I have found that being an HTL vs HT doesn't > necessarily mean that one > or the other can do the job better...I know a > few HTL's that were > grandfathered in and have done only a handful > IHCs in their whole > career, can't trouble shoot anything, and > produce poor quality slides > and yet they hold the title of an HTL and the > same goes for the > HTs...(makes you wonder how they keep a > job)...LOL....and I have taught > and worked with students right out of college > programs that were > absolutely awesome and knew their stuff....go > figure.....I say go for > it.....Good Luck.... > > > Kim Tournear, HT (ASCP), QIHC ( ASCP) > Specialists in Dermatology > Histology/Mohs Supervisor > Tucson, AZ > > > > > --------------------------------- > Boardwalk for $500? In 2007? Ha! > Play Monopoly Here and Now (it's updated for > today's economy) at Yahoo! > Games. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ---------------------------------------------------------------------------- > -- > The information contained in this message may > be privileged and confidential > and protected from disclosure. If the reader of > this message is not the > intended recipient, or an employee or agent > responsible for delivering this > message to the intended recipient, you are > hereby notified that any > dissemination, distribution or copying of this > communication is strictly > prohibited. If you have received this > communication in error, please notify > us immediately by replying to the message and > deleting it from your > computer. > Thank you. > ============================================================================ > == > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D ____________________________________________________________________________________ Yahoo! oneSearch: Finally, mobile search that gives answers, not web links. http://mobile.yahoo.com/mobileweb/onesearch?refer=1ONXIC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better Heartthrob. Get better relationship answers from someone who knows. Yahoo! Answers - Check it out. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Sep 13 11:34:30 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 13 11:34:44 2007 Subject: [Histonet] Histotech vacancies In-Reply-To: <24A4826E8EF0964D86BC5317306F58A502FEBB8586@mmc-mail.ad.mhsil.com> Message-ID: <995157.1476.qm@web61223.mail.yahoo.com> "On-line" histotech training programs, WITHOUT hands-on training, for me are worthless. They could give to the theory, but histology is more practice, than theory, although both should go hand-by-hand. What I did twice: hire a lab assistant, enroll it in a theory program and train it on the job. At the end the trained assistant will know the theory to pass the examinations, and will know the practice to do the job. Ren? J. "Vickroy, Jim" wrote: Like a lot of hospitals we have been unable to find qualified applicants for a histotech position. We are at least exploring other options. One of the options include hiring someone with a biological background and enrolling them in an on-line histotech training program. After completion of the program the applicant would take the certification examination. There are a few schools out there that have full time programs but many individuals are not in a position to take six months off to attend them without receiving a weekly paycheck. In addition my experience has been that these schools only take a small amount of applicants each session. So with more positions than students has anyone else come up with a creative way to train and hire histotechs and any suggestions to on-line programs that have been worthwhile. Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Fussy? Opinionated? Impossible to please? Perfect. Join Yahoo!'s user panel and lay it on us. From doug <@t> ppspath.com Thu Sep 13 12:37:21 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Thu Sep 13 11:37:55 2007 Subject: [Histonet] Histotech vacancies In-Reply-To: <24A4826E8EF0964D86BC5317306F58A502FEBB8586@mmc-mail.ad.mhsil.com> Message-ID: Good techs are hard to find and keeping them is even harder. It is all about supply and demand. What I see happening is that good techs are offered better wages at other local facilities. When more than one facility has an opening then it turns into a bidding war. In smaller towns/communities we all know who the good techs are. Word of mouth travels fast. Some administrators would rather let a good tech go then give them a $1 raise. Other places are more in tune with things and are offering wages that are higher than the local norm (which still below norm). If you hire someone and train them offering the online course then you better have them sign a "no compete" contract or something of that nature. If you don't then don't be surprised to see them take the training and then go to the highest bidder. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Thursday, September 13, 2007 11:15 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Histotech vacancies Like a lot of hospitals we have been unable to find qualified applicants for a histotech position. We are at least exploring other options. One of the options include hiring someone with a biological background and enrolling them in an on-line histotech training program. After completion of the program the applicant would take the certification examination. There are a few schools out there that have full time programs but many individuals are not in a position to take six months off to attend them without receiving a weekly paycheck. In addition my experience has been that these schools only take a small amount of applicants each session. So with more positions than students has anyone else come up with a creative way to train and hire histotechs and any suggestions to on-line programs that have been worthwhile. Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From m5johnso <@t> meded.ucsd.edu Thu Sep 13 11:51:23 2007 From: m5johnso <@t> meded.ucsd.edu (Mindy Johnson) Date: Thu Sep 13 11:51:40 2007 Subject: [Histonet] Histotech vacancies In-Reply-To: <24A4826E8EF0964D86BC5317306F58A502FEBB8586@mmc-mail.ad.mhsil.com> References: <24A4826E8EF0964D86BC5317306F58A502FEBB8586@mmc-mail.ad.mhsil.com> Message-ID: <00e401c7f626$51debb10$f59c3130$@ucsd.edu> I know when I was hired by my first job it was for a histology position and I had just graduated with a biology degree. I had some frozen sectioning experience while at school, but most of what I learned was on the job and they were going to have me take the online course since I was already cutting. Now, I'm doing even more cutting at my new job with paraffin and I'm learning still. But, still no license. So I am curious to see how these online courses workout for people. Maybe I should do one. ?Mindy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Thursday, September 13, 2007 9:15 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Histotech vacancies Like a lot of hospitals we have been unable to find qualified applicants for a histotech position. We are at least exploring other options. One of the options include hiring someone with a biological background and enrolling them in an on-line histotech training program. After completion of the program the applicant would take the certification examination. There are a few schools out there that have full time programs but many individuals are not in a position to take six months off to attend them without receiving a weekly paycheck. In addition my experience has been that these schools only take a small amount of applicants each session. So with more positions than students has anyone else come up with a creative way to train and hire histotechs and any suggestions to on-line programs that have been worthwhile. Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From STapper <@t> smdc.org Thu Sep 13 12:09:33 2007 From: STapper <@t> smdc.org (Tapper, Sheila J.) Date: Thu Sep 13 12:10:02 2007 Subject: [Histonet] Histotech vacancies In-Reply-To: <995157.1476.qm@web61223.mail.yahoo.com> Message-ID: IT?!! Sheila Tapper HT(ASCP) Anatomic Pathology Supervisor SMDC Clinical Laboratory 407 East First Street Duluth, MN 55804 Telephone: 218-786-5472 Fax: 218-786-2369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, September 13, 2007 11:35 AM To: Vickroy, Jim; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] Histotech vacancies "On-line" histotech training programs, WITHOUT hands-on training, for me are worthless. They could give to the theory, but histology is more practice, than theory, although both should go hand-by-hand. What I did twice: hire a lab assistant, enroll it in a theory program and train it on the job. At the end the trained assistant will know the theory to pass the examinations, and will know the practice to do the job. Ren? J. "Vickroy, Jim" wrote: Like a lot of hospitals we have been unable to find qualified applicants for a histotech position. We are at least exploring other options. One of the options include hiring someone with a biological background and enrolling them in an on-line histotech training program. After completion of the program the applicant would take the certification examination. There are a few schools out there that have full time programs but many individuals are not in a position to take six months off to attend them without receiving a weekly paycheck. In addition my experience has been that these schools only take a small amount of applicants each session. So with more positions than students has anyone else come up with a creative way to train and hire histotechs and any suggestions to on-line programs that have been worthwhile. Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Fussy? Opinionated? Impossible to please? Perfect. Join Yahoo!'s user panel and lay it on us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From relia1 <@t> earthlink.net Thu Sep 13 12:21:27 2007 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Sep 13 12:21:44 2007 Subject: [Histonet] RE:Histonet Vacancies May I add my 2 cents worth? Message-ID: Hi Histonetters, I know this is a problem that has plagued facilities for years and I too have noticed a change in the past 2 years. Yes, the histology programs nationwide produce a great albeit small group of talented people every year but the pool of available histo techs for permanent positions has shrunk even more in recent years. At the risk of being "flamed" by travel companies I have to say that you are losing alot of techs to travel positions. In the past 2 years of all of the histo techs I have had contact with over half only want to work in permanent positions the rest either want to continue as travelers or become travelers. Think about it... they get a higher rate of pay, benefits and living expenses paid for. For these people it is a "better deal" than committing to one facility. As a matter of fact it is a "better deal" than a temp/travel position in any other field outside of healthcare. Facilities who take the "quick solution" of hiring travel techs are contributing to the shortage. May I offer some solutions? Some creative hiring strategies? Here are some ideas I would like to share: 1. If you are using travel techs do it with a temp to perm clause - but be firm. If a tech works for you as a temp make sure they are at least considering converting to a permanent employee at the end of the contract. If not don't extend, have your travel company send someone else who would consider converting to a permanent position. And make questions about their intentions part of your interview process the same as you would if you were interviewing a candidate from out of state for a permanent position. 2. Human Resources - Many of your allied health recruiters don't seem to realize that histo techs don't grow on trees. So many times I see facilities lose great techs because the hiring process has dragged out and the candidate ends up taking a position with a facility that can move faster. Stay on top of your hr people especially once you know they have a histology candidate. 3. How about techs from Canada? There are alot of talented techs in Canada that are interested in moving to the states and the process is relatively easy due to NAFTA and the F1 visa. 4. How about techs that need sponsorship on an H-1 visa? I know alot of companies shy away from this alternative because of the length of time it can take to process a visa application but I think that if you take a look at the time it takes to find a tech at all against the time it would take to process an H-1 visa it is quickly becoming 6 of one vs. half dozen of another. I mean what difference does it make if it takes up to 8 weeks to process an H-1 visa vs. 2-3 months to identify a histology candidate? Your best bet is to get with your Human Resources department and strategize, educate them on the challenges and shortages you are facing. Discuss some of these options or others you might come up with. I hope this helps!! Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net From rjbuesa <@t> yahoo.com Thu Sep 13 12:43:31 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 13 12:43:44 2007 Subject: [Histonet] RE:Histonet Vacancies May I add my 2 cents worth? In-Reply-To: Message-ID: <561535.32414.qm@web61218.mail.yahoo.com> Your ending reminded me of Saturday Night Live and the "distressed" hostess urging to "discuss amongst yourselves!" Your first set of recommendations go against the nature of those who decide to "go traveler", the individuality of the decissions. The only real solution, unattainable though, is to get to a national pay scale for our profession, like in Canada, Australia, the UK, Austria, some countries of the EC, South Africa and others. Where the salaries are structured. By the way, I don't know why any Canadian colleague would like to come to the USA, since they are better paid than we are! Ren? J. Ren? J. Pam Barker wrote: Hi Histonetters, I know this is a problem that has plagued facilities for years and I too have noticed a change in the past 2 years. Yes, the histology programs nationwide produce a great albeit small group of talented people every year but the pool of available histo techs for permanent positions has shrunk even more in recent years. At the risk of being "flamed" by travel companies I have to say that you are losing alot of techs to travel positions. In the past 2 years of all of the histo techs I have had contact with over half only want to work in permanent positions the rest either want to continue as travelers or become travelers. Think about it... they get a higher rate of pay, benefits and living expenses paid for. For these people it is a "better deal" than committing to one facility. As a matter of fact it is a "better deal" than a temp/travel position in any other field outside of healthcare. Facilities who take the "quick solution" of hiring travel techs are contributing to the shortage. May I offer some solutions? Some creative hiring strategies? Here are some ideas I would like to share: 1. If you are using travel techs do it with a temp to perm clause - but be firm. If a tech works for you as a temp make sure they are at least considering converting to a permanent employee at the end of the contract. If not don't extend, have your travel company send someone else who would consider converting to a permanent position. And make questions about their intentions part of your interview process the same as you would if you were interviewing a candidate from out of state for a permanent position. 2. Human Resources - Many of your allied health recruiters don't seem to realize that histo techs don't grow on trees. So many times I see facilities lose great techs because the hiring process has dragged out and the candidate ends up taking a position with a facility that can move faster. Stay on top of your hr people especially once you know they have a histology candidate. 3. How about techs from Canada? There are alot of talented techs in Canada that are interested in moving to the states and the process is relatively easy due to NAFTA and the F1 visa. 4. How about techs that need sponsorship on an H-1 visa? I know alot of companies shy away from this alternative because of the length of time it can take to process a visa application but I think that if you take a look at the time it takes to find a tech at all against the time it would take to process an H-1 visa it is quickly becoming 6 of one vs. half dozen of another. I mean what difference does it make if it takes up to 8 weeks to process an H-1 visa vs. 2-3 months to identify a histology candidate? Your best bet is to get with your Human Resources department and strategize, educate them on the challenges and shortages you are facing. Discuss some of these options or others you might come up with. I hope this helps!! Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Luggage? GPS? Comic books? Check out fitting gifts for grads at Yahoo! Search. From elizabeth.heimrich <@t> bms.com Thu Sep 13 13:00:22 2007 From: elizabeth.heimrich <@t> bms.com (Elizabeth M Heimrich) Date: Thu Sep 13 13:01:12 2007 Subject: [Histonet] Histotech vacancies In-Reply-To: <24A4826E8EF0964D86BC5317306F58A502FEBB8586@mmc-mail.ad.mhsil.com> References: <24A4826E8EF0964D86BC5317306F58A502FEBB8586@mmc-mail.ad.mhsil.com> Message-ID: <46E97AB6.70409@bms.com> Jim, I fall into that catagory of histotechs... BS in Biology w/15 yrs histology experience. I went through a distance learning program via Indiana University. I thought I knew histology until I took the class. What an intense class!! With this program you the employer must be willing to give the tech time during the day to "go to class" in a conference room somewhere quiet, and be willing to give them time/space in the lab to do the practical portion of the class. It is a full year program with lab and theory classes. Like I said it is intense, but it prepares the student to take the HT/ HTL exam. I have not taken the exam, as I have decided to persue my Master's degree. But I agree with you, there are less people entering into histology (clinical) possibly because of the testing required? Research is less stringent, but there are less opportunities for histo techs. If you and/or anyone else want the contact info for this program send me an email and I will send it to you. Best regards, Beth Vickroy, Jim wrote: >Like a lot of hospitals we have been unable to find qualified applicants for a histotech position. We are at least exploring other options. One of the options include hiring someone with a biological background and enrolling them in an on-line histotech training program. After completion of the program the applicant would take the certification examination. There are a few schools out there that have full time programs but many individuals are not in a position to take six months off to attend them without receiving a weekly paycheck. In addition my experience has been that these schools only take a small amount of applicants each session. So with more positions than students has anyone else come up with a creative way to train and hire histotechs and any suggestions to on-line programs that have been worthwhile. > > > >Jim Vickroy >Technical Supervisor - Surgical and Autopsy Pathology >Memorial Medical Center > > > >This message (including any attachments) contains confidential information intended for a > >specific individual and purpose, and is protected by law. If you are not the intended recipient, > >you should delete this message. Any disclosure, copying, or distribution of this message, or the > >taking of any action based on it, is strictly prohibited. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Shirley_PHUA <@t> hsa.gov.sg Thu Sep 13 13:02:13 2007 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Thu Sep 13 13:04:47 2007 Subject: [Histonet] Shirley Phua is away on 14 Sep 2007 (Friday). Message-ID: I will be out of the office from 14-09-2007 to 14-09-2007. I'll be away on 14 Sep 2007 (Friday). I'll be back on 17 Sep 2007 (Monday). Pathologists: I will process your requests when I return. If urgent, please forward your email to Henry_Kyaw@hsa.gov.sg From HSteele <@t> uwhealth.org Thu Sep 13 13:19:08 2007 From: HSteele <@t> uwhealth.org (Steele Heather) Date: Thu Sep 13 13:20:23 2007 Subject: [Histonet] Weigert myelin stain procedure Message-ID: Does anyone have a good procedure on hand for Weigert's myelin stain??!! Thanks, Heather Steele, HTL University of Wisconsin Hospital and Clinics From collette2 <@t> mail.llnl.gov Thu Sep 13 14:20:07 2007 From: collette2 <@t> mail.llnl.gov (Nicole Collette) Date: Thu Sep 13 14:15:10 2007 Subject: [Histonet] alizarin red S mouse embryo alternate protocol question Message-ID: Hello, All, I am trying to do skeletal stains with whole mouse embryos E14.5-E16.5 with alizarin only to look at gross mineralization of bones (the alcian blue interferes with interpretation since the cartilage and bone overlay each other, and this experiment should be straightforward enough not to require sectioning). I have tried this stain with a similar protocol to what we use for adults, which calls for alizarin in KOH, followed by KOH digestion and clearing. When I tried this on that same ages of embryos recently, I got heads that exploded, presumably due to some osmotic issue (happened during staining, not during subsequent digestion/clearing), and despite watching the embryos very carefully not to overdigest, they overdigested and were essentially useless. I found the Arnott protocol in the Atlas of Mouse Development, that calls for fixation in ethanol, dehydration/lipid dissolving in acetone, staining in ethanol, then KOH. When I put the alizarin in acid alcohol, I get a yellow solution (that dissolves poorly in ethanol, too), is KOH required to make it then turn red/purple? Would it be better to try a more gradual way to negotiate the change from 95% ethanol to 1% KOH? I also found a modification of this protocol that doesn't use KOH at all, but it's in a journal I can't get my hands on, reference is below. I am concerned that if I stain in acid alcohol, dehydrate/clear in ethanol:glycerol instead of KOH/glycerol, I won't get a color change. Perhaps some combination of high pH/ethanol would be better? Any help would be most appreciated... Thanks in advance for the help! Nicole Teratology. 1980 Dec;22(3):299-301.Links Differential staining of cartilage and bone in whole mouse fetuses by alcian blue and alizarin red S. McLeod MJ. The procedure described by Inouye ('76) for the staining of full-term mouse fetal skeletons has been adapted for use with mouse embryos and fetuses of days 14-18 of gestation. The main adaptations for younger specimens involve a longer time in acetone, in lieu of skinning, and omission of the aqueous KOH step. These adaptations require more time but result in consistently good staining of intact specimens. PMID: 6165088 [PubMed - indexed for MEDLINE] From mlm11 <@t> cornell.edu Thu Sep 13 14:49:48 2007 From: mlm11 <@t> cornell.edu (Mary Lou Norman) Date: Thu Sep 13 14:50:00 2007 Subject: [Histonet] stereomounts Message-ID: <6.2.1.2.2.20070913153006.0418b3f0@postoffice9.mail.cornell.edu> Hello Histonet, Has everybody gone digital or is there someone out there interested in 3-D stereomounts? I have glass and aluminum frames. Emde and Realist. Thanks, Mary Lou From jm.lapointe <@t> accellab.com Thu Sep 13 15:12:49 2007 From: jm.lapointe <@t> accellab.com (Jean-Martin Lapointe) Date: Thu Sep 13 15:14:50 2007 Subject: [Histonet] Azan-Mallory References: <000b01c7f618$d353f010$6500a8c0@mainbox> Message-ID: Thanks all, this explains my not finding it as spelled. We will try the Heidenhain's Azan and see what we get. Jean-Martin Lapointe -----Original Message----- From: Bryan Hewlett [mailto:bhewlett@cogeco.ca] Sent: 13 septembre 2007 11:15 To: Jean-Martin Lapointe; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Azan-Mallory Hi Jean-Martin, The reason that you cannot locate the Azan-Mallory stain is because you have been given the wrong name! The stain is actually Heidenhain's 'Azan' modification of Mallory's connective tissue stain (trichrome). A google search for 'Heidenhain's azan' will get you this website with the method. http://stainsfile.info/StainsFile/stain/conektv/tri_heidenhain.htm Regards, Bryan ----- Original Message ----- From: "Jean-Martin Lapointe" To: Sent: Thursday, September 13, 2007 10:46 AM Subject: [Histonet] Azan-Mallory Greetings, My lab has been asked to do Azan-Mallory stains for a study. I cannot find a technique anywhere for this stain - not in the histotech books we have, not on various web sites listing histo techniques, not in the literature. Many literature papers cite the use of this stain as if it was a standard method, without any reference. I am wondering if it could be another name for a commonly used stain. Can anyone help ? _________________________________ Jean-Martin Lapointe, DMV, MS, dACVP AccelLAB Inc _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- This message has been verified by LastSpam eMail security service Ce courriel a ?t? v?rifi? par le service de s?curit? pour courriels LastSpam http://www.lastspam.com From tdobersztyn <@t> chmca.org Thu Sep 13 15:14:40 2007 From: tdobersztyn <@t> chmca.org (tdobersztyn@chmca.org) Date: Thu Sep 13 15:14:59 2007 Subject: [Histonet] Need help with Brain Tumor FS In-Reply-To: <20070913131804.E7FFA585B@interceptor.chmca.org> Message-ID: Hello All! Can anyone recommend a protocol for frozen section technique - to cut Brain tumor biopsies. The usual ice-crystal artifact sometimes renders the Frozen Sections unreadable for intra-operative diagnosis. We use a Leica UV1850 cryostat set at -21* OCT media and cut our FS at 8 microns then: rapid stain H/E The pathologist here tends to believe it is our technique that produces the artifact. fast freezing with spray vs slow freezing?????????? We have tried a lot of methods to eliminate this problem HELP!!!!!!!!!!! I know someone out there has a remedy for the headache this has created!!!! Thank you all in advance!!! I am glad I can pose questions to others who might be dealing with the issues I deal with too! Theresa From Jessica.Vacca <@t> HCAhealthcare.com Thu Sep 13 15:40:00 2007 From: Jessica.Vacca <@t> HCAhealthcare.com (Vacca Jessica) Date: Thu Sep 13 15:41:26 2007 Subject: [Histonet] Surgical specimens Message-ID: <41E16A15CE78374EA45B57E0F94339B802C58797@ORLEV01.hca.corpad.net> Those of you that are hospital based........... Does surgery bring their surgical specimens to you or do you go to surgery to pick them up? We currently go to surgery, but since we are so short staffed...Did I mention we have a FT HT/HTL position available???? I'm going to present to my Lab director to see if I can get a "circulator" to deliver the specimens? Just wanted to get a consensus. Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Drive Brandon,FL 33511 813-571-5193 813-571-5169 Fax From igor.deyneko <@t> gmail.com Thu Sep 13 16:00:07 2007 From: igor.deyneko <@t> gmail.com (Igor Deyneko) Date: Thu Sep 13 16:00:20 2007 Subject: [Histonet] Histology Atlas Message-ID: <35e16a770709131400k671f9dby68b94146decb63cc@mail.gmail.com> Dear Histonetters! I am looking for a good atlas of murine(and rat) histology, preferably presenting all different types of tissues. Can anyone suggest a good one to me. Thank you very much. Igor Deyneko Infinity Pharmaceuticals Cambridge,MA From tkngflght <@t> yahoo.com Thu Sep 13 16:01:43 2007 From: tkngflght <@t> yahoo.com (Cheryl R. Kerry) Date: Thu Sep 13 16:01:35 2007 Subject: [Histonet] Surgical specimens In-Reply-To: <41E16A15CE78374EA45B57E0F94339B802C58797@ORLEV01.hca.corpad.net> Message-ID: <004001c7f649$4a2bd2e0$6701a8c0@CHERYLSLAPTOP> Hi Jessica- I've been in facilities that handled it either way--and some that did both depending on the staffing situation and sometimes the nurse staff ran stuff by on their way home. As long as there was consistency in being checked before being checked in, it went well. Orienting the new folks to the procedure was the most important part of the deal and having a laminated 'check list' sort of reminder with an example posted at the log did wonders to keep the problems in ID and mislabeling under control. Having the runner (circulator, tech, nurse) sign stuff in (keeping them responsible) was an important part of the process. My two-cents! Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc 281.852.9457 Staffing the AP Lab - One great tech at a time! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vacca Jessica Sent: Thursday, September 13, 2007 3:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Surgical specimens Those of you that are hospital based........... Does surgery bring their surgical specimens to you or do you go to surgery to pick them up? We currently go to surgery, but since we are so short staffed...Did I mention we have a FT HT/HTL position available???? I'm going to present to my Lab director to see if I can get a "circulator" to deliver the specimens? Just wanted to get a consensus. Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Drive Brandon,FL 33511 813-571-5193 813-571-5169 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Thu Sep 13 16:22:47 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Sep 13 16:23:08 2007 Subject: [Histonet] Histotech vacancies References: <24A4826E8EF0964D86BC5317306F58A502FEBB8586@mmc-mail.ad.mhsil.com> <00e401c7f626$51debb10$f59c3130$@ucsd.edu> Message-ID: <00a101c7f64c$3d769f00$0202a8c0@yourxhtr8hvc4p> on line courses can work if the clinical sites do what they are supposed to do. For instance, at my last place of employment, students were cutting embedded blocks on day 2. I would process some extra tissue and let the students embed and cut. (day 1 was safety, trip around the lab, ect.), by week 5, they were cutting 1 slide surgical and doing all the special stains. Now, this was for the CC program here in San Antonio, but, my students were reporting back to me that their classmates haven't sat down at a microtome. So, the moral of the story is that on line programs are only as good as the clinical sites. That's my story and I'm sticking to it!!! JTT ----- Original Message ----- From: "Mindy Johnson" To: "'Vickroy, Jim'" ; Sent: Thursday, September 13, 2007 11:51 AM Subject: RE: [Histonet] Histotech vacancies I know when I was hired by my first job it was for a histology position and I had just graduated with a biology degree. I had some frozen sectioning experience while at school, but most of what I learned was on the job and they were going to have me take the online course since I was already cutting. Now, I'm doing even more cutting at my new job with paraffin and I'm learning still. But, still no license. So I am curious to see how these online courses workout for people. Maybe I should do one. Mindy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Thursday, September 13, 2007 9:15 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Histotech vacancies Like a lot of hospitals we have been unable to find qualified applicants for a histotech position. We are at least exploring other options. One of the options include hiring someone with a biological background and enrolling them in an on-line histotech training program. After completion of the program the applicant would take the certification examination. There are a few schools out there that have full time programs but many individuals are not in a position to take six months off to attend them without receiving a weekly paycheck. In addition my experience has been that these schools only take a small amount of applicants each session. So with more positions than students has anyone else come up with a creative way to train and hire histotechs and any suggestions to on-line programs that have been worthwhile. Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Thu Sep 13 16:26:21 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Sep 13 16:26:42 2007 Subject: [Histonet] Need help with Brain Tumor FS References: Message-ID: <00b701c7f64c$bde94fc0$0202a8c0@yourxhtr8hvc4p> have you tried liquid nitrogen? JTT ----- Original Message ----- From: To: Sent: Thursday, September 13, 2007 3:14 PM Subject: [Histonet] Need help with Brain Tumor FS > Hello All! > > Can anyone recommend a protocol for frozen section technique - to cut > Brain tumor biopsies. > > The usual ice-crystal artifact sometimes renders the Frozen Sections > unreadable for intra-operative diagnosis. > > We use a Leica UV1850 cryostat set at -21* > OCT media > and cut our FS at 8 microns > then: > rapid stain H/E > > The pathologist here tends to believe it is our technique that produces > the artifact. > > fast freezing with spray vs slow freezing?????????? > > We have tried a lot of methods to eliminate this problem > > HELP!!!!!!!!!!! I know someone out there has a remedy for the headache > this has created!!!! > > > Thank you all in advance!!! > I am glad I can pose questions to others who might be dealing with the > issues I deal with too! > > Theresa > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Thu Sep 13 16:25:16 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Sep 13 16:26:43 2007 Subject: [Histonet] RE:Histonet Vacancies May I add my 2 cents worth? References: Message-ID: <00b201c7f64c$bb67fc10$0202a8c0@yourxhtr8hvc4p> a few years ago when nursing had a severe shortage, the nursing organizations, companies, hospitals and what ever went to other countries to recruit. Maybe it time we did too. JTT ----- Original Message ----- From: "Pam Barker" To: "'Histonet'" Sent: Thursday, September 13, 2007 12:21 PM Subject: [Histonet] RE:Histonet Vacancies May I add my 2 cents worth? > Hi Histonetters, > I know this is a problem that has plagued facilities for years and I too > have noticed a change in the past 2 years. Yes, the histology programs > nationwide produce a great albeit small group of talented people every > year but the pool of available histo techs for permanent positions has > shrunk even more in recent years. At the risk of being "flamed" by > travel companies I have to say that you are losing alot of techs to > travel positions. In the past 2 years of all of the histo techs I have > had contact with over half only want to work in permanent positions the > rest either want to continue as travelers or become travelers. Think > about it... they get a higher rate of pay, benefits and living expenses > paid for. For these people it is a "better deal" than committing to one > facility. As a matter of fact it is a "better deal" than a temp/travel > position in any other field outside of healthcare. Facilities who take > the "quick solution" of hiring travel techs are contributing to the > shortage. May I offer some solutions? Some creative hiring strategies? > > Here are some ideas I would like to share: > 1. If you are using travel techs do it with a temp to perm clause - but > be firm. If a tech works for you as a temp make sure they are at least > considering converting to a permanent employee at the end of the > contract. If not don't extend, have your travel company send someone > else who would consider converting to a permanent position. And make > questions about their intentions part of your interview process the same > as you would if you were interviewing a candidate from out of state for > a permanent position. > > 2. Human Resources - Many of your allied health recruiters don't seem to > realize that histo techs don't grow on trees. So many times I see > facilities lose great techs because the hiring process has dragged out > and the candidate ends up taking a position with a facility that can > move faster. Stay on top of your hr people especially once you know > they have a histology candidate. > > 3. How about techs from Canada? There are alot of talented techs in > Canada that are interested in moving to the states and the process is > relatively easy due to NAFTA and the F1 visa. > > 4. How about techs that need sponsorship on an H-1 visa? I know alot of > companies shy away from this alternative because of the length of time > it can take to process a visa application but I think that if you take a > look at the time it takes to find a tech at all against the time it > would take to process an H-1 visa it is quickly becoming 6 of one vs. > half dozen of another. I mean what difference does it make if it takes > up to 8 weeks to process an H-1 visa vs. 2-3 months to identify a > histology candidate? > > Your best bet is to get with your Human Resources department and > strategize, educate them on the challenges and shortages you are facing. > Discuss some of these options or others you might come up with. > I hope this helps!! > > Thank You! > > > Pam Barker > President > RELIA > Specialists in Allied Healthcare Recruiting > 5703 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell: (407)353-5070 > FAX: (407)678-2788 > E-mail: relia1@earthlink.net > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Thu Sep 13 16:32:42 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Sep 13 16:32:11 2007 Subject: [Histonet] AO 860 sliding microtome and Caroline Bass Message-ID: <6.0.0.22.1.20070913151904.01b126f8@gemini.msu.montana.edu> Caroline, If you send me your slowmail, US postal service address, I will send you a manual on the effective use and proper care of the microtome. This little green booklet (collectors item!) tells how to operate the 860 along with other AO microtomes (820 and freezing microtome). I found an extra copy in my file this past month. As for disposable blades in this sliding microtome, we always used a c profile steel knife, a gigantic blade 200 mm long, for study, vibration free operation. The clamp may not work with a disposable holder. Dorn and Hart is a good place to get resharpening/reconditioning of the blades or do that with a ThermoShandon whatever name now knife sharpener. This was a superb sharpener and the microtome is a oldie but a goodie, heavy duty finger amputator. We also used Bear Oil, a microtome oil which is still available although Rene's suggestion for the Leica oil is ideal too. Be careful, these microtomes are very efficient for sectioning and cutting YOU!!! It was the first sliding microtome I used for sectioning celloidin embedded monkey temporal mandibular joints back in 1963. It was fun, but I did work with closed doors during its operation to avoid the startle factor during sectioning. I kept my fingers, arms, etc intact. Good luck with your "new" toy. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From gcallis <@t> montana.edu Thu Sep 13 16:40:03 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Sep 13 16:39:30 2007 Subject: [Histonet] Re: Need help with Brain Tumor FS In-Reply-To: <00b701c7f64c$bde94fc0$0202a8c0@yourxhtr8hvc4p> References: <00b701c7f64c$bde94fc0$0202a8c0@yourxhtr8hvc4p> Message-ID: <6.0.0.22.1.20070913153339.01afd530@gemini.msu.montana.edu> Theresa, Joe's suggestion on snap freezing is excellent to avoid freezing artifact, but you need to discuss this with Nancy Lemke at Nperson211@comcast.net. She also works with brain tumor biopsies and has experienced your problems too. She can give you even more hints since she is involved with brain tissue biopsies for both clinical and research. She can also provide information on optimal temperature setting for these biopsies/tumor samples. If Nancy has already replied, please forgive me, I have been off Histonet for weeks. Good luck Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman Bozeman MT 59717-3610 At 03:26 PM 9/13/2007, you wrote: >have you tried liquid nitrogen? > >JTT >----- Original Message ----- From: >To: >Sent: Thursday, September 13, 2007 3:14 PM >Subject: [Histonet] Need help with Brain Tumor FS > > >>Hello All! >>Can anyone recommend a protocol for frozen section technique - to cut >>Brain tumor biopsies. >>The usual ice-crystal artifact sometimes renders the Frozen Sections >>unreadable for intra-operative diagnosis. >>We use a Leica UV1850 cryostat set at -21* >>OCT media and cut our FS at 8 microns >>then: >>rapid stain H/E >>The pathologist here tends to believe it is our technique that produces >>the artifact. >>fast freezing with spray vs slow freezing?????????? >>We have tried a lot of methods to eliminate this problem >>HELP!!!!!!!!!!! I know someone out there has a remedy for the headache >>this has created!!!! >> From tim.morken <@t> thermofisher.com Thu Sep 13 16:48:41 2007 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Thu Sep 13 16:49:16 2007 Subject: [Histonet] Histotech vacancies In-Reply-To: <00a101c7f64c$3d769f00$0202a8c0@yourxhtr8hvc4p> References: <24A4826E8EF0964D86BC5317306F58A502FEBB8586@mmc-mail.ad.mhsil.com><00e401c7f626$51debb10$f59c3130$@ucsd.edu> <00a101c7f64c$3d769f00$0202a8c0@yourxhtr8hvc4p> Message-ID: <6BFF6D137DF6BC43B33891BA96E83B19BBF478@PGHCR-EXMB-VS-1.na.fshrnet.com> The online courses should be much more helpful than studying alone. Most of us spent hundreds of hours poring through books on our own since there are few formal courses available (and was long before an "internet" was around). I was lucky enough to have 3 other people around who wanted to get their HT/HTL and we had a study group going weekly for a solid year before we took the exam. Most labs don't do everything presented on the test and a formal course will be sure they get that theory in a well-structured way. Of course the lab work is a must to put it all in practice. Tim Morken Technical Support Manager Anatomical Pathology, Lab Vision Products ThermoFisher Scientific -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Thursday, September 13, 2007 2:23 PM To: Mindy Johnson; 'Vickroy, Jim'; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Histotech vacancies on line courses can work if the clinical sites do what they are supposed to do. For instance, at my last place of employment, students were cutting embedded blocks on day 2. I would process some extra tissue and let the students embed and cut. (day 1 was safety, trip around the lab, ect.), by week 5, they were cutting 1 slide surgical and doing all the special stains. Now, this was for the CC program here in San Antonio, but, my students were reporting back to me that their classmates haven't sat down at a microtome. So, the moral of the story is that on line programs are only as good as the clinical sites. That's my story and I'm sticking to it!!! JTT ----- Original Message ----- From: "Mindy Johnson" To: "'Vickroy, Jim'" ; Sent: Thursday, September 13, 2007 11:51 AM Subject: RE: [Histonet] Histotech vacancies I know when I was hired by my first job it was for a histology position and I had just graduated with a biology degree. I had some frozen sectioning experience while at school, but most of what I learned was on the job and they were going to have me take the online course since I was already cutting. Now, I'm doing even more cutting at my new job with paraffin and I'm learning still. But, still no license. So I am curious to see how these online courses workout for people. Maybe I should do one. Mindy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Thursday, September 13, 2007 9:15 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Histotech vacancies Like a lot of hospitals we have been unable to find qualified applicants for a histotech position. We are at least exploring other options. One of the options include hiring someone with a biological background and enrolling them in an on-line histotech training program. After completion of the program the applicant would take the certification examination. There are a few schools out there that have full time programs but many individuals are not in a position to take six months off to attend them without receiving a weekly paycheck. In addition my experience has been that these schools only take a small amount of applicants each session. So with more positions than students has anyone else come up with a creative way to train and hire histotechs and any suggestions to on-line programs that have been worthwhile. Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Thu Sep 13 16:59:41 2007 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Sep 13 17:01:33 2007 Subject: [Histonet] Need help with Brain Tumor FS Message-ID: Theresa, The problem is probably not your technique. Are your samples received swimming in saline? I would be confident this is your problem. I have just had accepted a paper entitled "Adverse Effect of Saline on Brain Intra-Operative (Frozen Section) Histology" which should appear in the next issue of the Journal of Histotechnology that illustrates this artefact. Request that your surgeons send their specimens dry on moist card or gauze. We also immediately fix our sections in methanol prior to rapid HE staining, rather than air-drying. It produces a result very similar to a paraffin section HE. Try it. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of tdobersztyn@chmca.org Sent: Friday, 14 September 2007 6:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Need help with Brain Tumor FS Hello All! Can anyone recommend a protocol for frozen section technique - to cut Brain tumor biopsies. The usual ice-crystal artifact sometimes renders the Frozen Sections unreadable for intra-operative diagnosis. We use a Leica UV1850 cryostat set at -21* OCT media and cut our FS at 8 microns then: rapid stain H/E The pathologist here tends to believe it is our technique that produces the artifact. fast freezing with spray vs slow freezing?????????? We have tried a lot of methods to eliminate this problem HELP!!!!!!!!!!! I know someone out there has a remedy for the headache this has created!!!! Thank you all in advance!!! I am glad I can pose questions to others who might be dealing with the issues I deal with too! Theresa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From algranth <@t> u.arizona.edu Thu Sep 13 17:21:38 2007 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Thu Sep 13 17:25:39 2007 Subject: [Histonet] tissue of the week - shrew mandibles Message-ID: <6.2.3.4.1.20070913151703.01f92b38@algranth.inbox.email.arizona.edu> Does anybody have a protocol for decalcification and processing of shrew mandibles? They are just a little over a centimeter in length and not very thick so I don't think the decaling would be very long. Haven't done this type of tissue before so I'm wondering if anybody out there has some hints for sectioning. The person who brought them said they could be hard to cut. Thanks. Andi ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From sccrshlly <@t> yahoo.com Thu Sep 13 17:49:38 2007 From: sccrshlly <@t> yahoo.com (Shelly Coker) Date: Thu Sep 13 17:49:48 2007 Subject: [Histonet] Re: Histotech vacancies Message-ID: <723218.56134.qm@web90305.mail.mud.yahoo.com> I actually enrolled in one of the online programs. The program I enrolled in required a "mentoring" laboratory, where I was assigned a mentor. Long story short, I performed the technical work for the course in my lab and mailed it to the program director. In addition, I had online coursework to do as well. It must have worked, because I passed my registry with flying colors on the first try (that was when it was the computer adaptive test and the practical). The only caution that I would give regarding these online courses is that you only get out what you put in. But then, most things in life are that way! Shelly --------------------------------- Boardwalk for $500? In 2007? Ha! Play Monopoly Here and Now (it's updated for today's economy) at Yahoo! Games. From jnocito <@t> satx.rr.com Thu Sep 13 18:10:59 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Sep 13 18:11:12 2007 Subject: [Histonet] tissue of the week - shrew mandibles References: <6.2.3.4.1.20070913151703.01f92b38@algranth.inbox.email.arizona.edu> Message-ID: <002501c7f65b$590cdae0$0202a8c0@yourxhtr8hvc4p> Andi, would that taming of the shrew!!! I'm such the knee slapper, I kill myself. Back to your question, no, I don't have a procedure. JTT ----- Original Message ----- From: "Andrea Grantham" To: Sent: Thursday, September 13, 2007 5:21 PM Subject: [Histonet] tissue of the week - shrew mandibles > Does anybody have a protocol for decalcification and processing of > shrew mandibles? > They are just a little over a centimeter in length and not very thick > so I don't think the decaling would be very long. > Haven't done this type of tissue before so I'm wondering if anybody > out there has some hints for sectioning. The person who brought them > said they could be hard to cut. > > Thanks. > > Andi > ..................................................................... > : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : > : Sr. Research Specialist University of Arizona : > : (office: AHSC 4212) P.O. Box 245044 : > : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : > : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : > :...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> u.arizona.edu Thu Sep 13 18:46:30 2007 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Thu Sep 13 18:50:34 2007 Subject: [Histonet] tissue of the week - shrew mandibles In-Reply-To: <002501c7f65b$590cdae0$0202a8c0@yourxhtr8hvc4p> References: <6.2.3.4.1.20070913151703.01f92b38@algranth.inbox.email.arizona.edu> <002501c7f65b$590cdae0$0202a8c0@yourxhtr8hvc4p> Message-ID: <6.2.3.4.1.20070913164500.01f7c728@algranth.inbox.email.arizona.edu> JTT, They have been tamed - in fact they are sleeping nicely over the weekend in cassettes waiting for their dunk into decal on Monday morning. Andi At 04:10 PM 9/13/2007, Joe Nocito wrote: >Andi, >would that taming of the shrew!!! I'm such the knee slapper, I kill myself. >Back to your question, no, I don't have a procedure. > >JTT >----- Original Message ----- From: "Andrea Grantham" >To: >Sent: Thursday, September 13, 2007 5:21 PM >Subject: [Histonet] tissue of the week - shrew mandibles > > >>Does anybody have a protocol for decalcification and processing of >>shrew mandibles? >>They are just a little over a centimeter in length and not very >>thick so I don't think the decaling would be very long. >>Haven't done this type of tissue before so I'm wondering if anybody >>out there has some hints for sectioning. The person who brought >>them said they could be hard to cut. >>Thanks. >>Andi >>..................................................................... >>: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : >>: Sr. Research Specialist University of Arizona : >>: (office: AHSC 4212) P.O. Box 245044 : >>: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : >>: (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : >>:...................................................................: >> http://www.cba.arizona.edu/histology-lab.html >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From lpwenk <@t> sbcglobal.net Thu Sep 13 19:56:56 2007 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Sep 13 19:57:25 2007 Subject: [Histonet] Histotech vacancies In-Reply-To: <24A4826E8EF0964D86BC5317306F58A502FEBB8586@mmc-mail.ad.mhsil.com> Message-ID: <001901c7f66a$263d5b30$0202a8c0@HPPav2> A couple of comments on this topic, dear to my heart, of course. First, the good news is that we are up to 32 HT and HTL NAACLS accredited programs in the US. I know that's not a lot, but a few years ago, we were down to 21! So a big hand to those programs who have survived, and to those who have just started up in the last half dozen years. Aye! Second of all, most of the new programs are college based, so they accept and train more students per year than the 1-4 students trained by most hospital based programs. So more students are being trained each year. Third, several NAAClS accredited histotech programs have gone to on-line and/or teleconference teaching. These programs do the theory training over the internet, email, teleconferences, etc. But the "students" are people in real histology labs around the US, already working as your own histotechs or trainees. In addition to doing the required reading, attending "class" via internet/etc, mailing in homework, taking exams, these students also have to cut their own slides, do their own staining, and mail these sets into the colleges for evaluation. They get evaluated by the college, and have to turn in sets without knife lines, no folds, H&E with nuclear pattern and 3 shades of eosin, trichromes where the muscle is red red and the connective tissue is blue blue, etc. Their supervisor monitors their taking the exams and gives them time to complete and send in all the slide sets. So many more students can be trained via web-based or teleconferences at these NAACLS accredited HT/HTL programs, and in parts of the country were there are no "regular" NAACLS HT/HTL programs. Now, a little commercial - if you are interested, I'm giving a workshop at the NSH Symposium on how to start a NAACLS accredited HT/HTL program, #31, Sunday Oct. 28, Sunday, 1-4:30 pm. I'd love to have 50 HT/HTL programs around the US in a few more years. Come yourself, and learn how to set up various types of programs. Contact your local community college or university, and let them know about the shortages, and invite them to send someone to the workshop. If there is a MT or MLT program at your nearby college/university, definitely contact them and invite them to attend. We're all accredited by the same organization, and the college classes are often the same - anatomy, physiology, microbiology, chemistry, etc. I think they would be thrilled to open up another avenue for their students. (P.S. No, I don't get a commission based on how many people attend. I just want to help get more HT/HTL programs started, so that we don't have this problem of shortages and poorly trained histotechs.) Peggy A. Wenk, HTL(ASCP)SLS Program Director Schools of Histotechnology William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Thursday, September 13, 2007 12:15 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Histotech vacancies Like a lot of hospitals we have been unable to find qualified applicants for a histotech position. We are at least exploring other options. One of the options include hiring someone with a biological background and enrolling them in an on-line histotech training program. After completion of the program the applicant would take the certification examination. There are a few schools out there that have full time programs but many individuals are not in a position to take six months off to attend them without receiving a weekly paycheck. In addition my experience has been that these schools only take a small amount of applicants each session. So with more positions than students has anyone else come up with a creative way to train and hire histotechs and any suggestions to on-line programs that have been worthwhile. Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From collette2 <@t> mail.llnl.gov Thu Sep 13 20:27:39 2007 From: collette2 <@t> mail.llnl.gov (Nicole Collette) Date: Thu Sep 13 20:22:47 2007 Subject: [Histonet] alizarin red S mouse embryo alternate protocol question In-Reply-To: References: Message-ID: Hello, Tamara, The protocol calls for 1% KOH. I could drop it down, but I was also concerned about osmotic issues with transitioning from 95% ethanol straight into full aqueous solution. I have done the protocol with E17.5 embryos (bone and cartilage), and they survive just fine, I basically stain in 1% KOH O/N and skip all other KOH steps to avoid overdigestion of those little guys. When I tried it with these younger guys they looked OK after staining for a few hours (not O/N) except for the heads exploding, and then during subsequent clearing in glycerol (no KOH) they fell apart. Thanks for your advice and help. Nicole >Nicole >what percentage of KOH are you using. we use trypsin for our digestion >step and fix in buffered formalin or paraformaldehyde. I routinely stain >chicken embryos for bone and/or cartilage. >Tamara > > > >Tamara Franz-Odendaal (PhD) >Assistant Professor, Biology, MSVU > >Mount Saint Vincent University >166 Bedford Highway >Halifax, NS, B3M 2J6 >Canada >Tel: +1 902 - 457 6140 (office) >Tel: +1 902 - 457 6718 (lab) >Fax: +1 902 - 457 6455 >http://faculty.msvu.ca/tfodendaal/ > >>>> Nicole Collette 09/13/07 4:20 PM >>> >Hello, All, > >I am trying to do skeletal stains with whole mouse embryos >E14.5-E16.5 with alizarin only to look at gross mineralization of >bones (the alcian blue interferes with interpretation since the >cartilage and bone overlay each other, and this experiment should be >straightforward enough not to require sectioning). I have tried this >stain with a similar protocol to what we use for adults, which calls >for alizarin in KOH, followed by KOH digestion and clearing. When I >tried this on that same ages of embryos recently, I got heads that >exploded, presumably due to some osmotic issue (happened during >staining, not during subsequent digestion/clearing), and despite >watching the embryos very carefully not to overdigest, they >overdigested and were essentially useless. I found the Arnott >protocol in the Atlas of Mouse Development, that calls for fixation >in ethanol, dehydration/lipid dissolving in acetone, staining in >ethanol, then KOH. When I put the alizarin in acid alcohol, I get a >yellow solution (that dissolves poorly in ethanol, too), is KOH >required to make it then turn red/purple? Would it be better to try a >more gradual way to negotiate the change from 95% ethanol to 1% KOH? >I also found a modification of this protocol that doesn't use KOH at >all, but it's in a journal I can't get my hands on, reference is >below. I am concerned that if I stain in acid alcohol, >dehydrate/clear in ethanol:glycerol instead of KOH/glycerol, I won't >get a color change. Perhaps some combination of high pH/ethanol would >be better? Any help would be most appreciated... > >Thanks in advance for the help! >Nicole > > > > Teratology. 1980 Dec;22(3):299-301.Links > Differential staining of cartilage and bone in whole mouse >fetuses by alcian blue and alizarin red S. > McLeod MJ. > > The procedure described by Inouye ('76) for the staining of >full-term mouse fetal skeletons has been adapted for use with mouse >embryos and fetuses of days 14-18 of gestation. The main adaptations >for younger specimens involve a longer time in acetone, in lieu of >skinning, and omission of the aqueous KOH step. These adaptations >require more time but result in consistently good staining of intact >specimens. > > PMID: 6165088 [PubMed - indexed for MEDLINE] >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >This communication, including any attached documentation, is >intended only for the person or entity to which it is addressed, and >may contain confidential, personal, and/or privileged information. >Any unauthorized disclosure, copying, or taking action on the >contents is strictly prohibited. If you have received this message >in error, please contact us immediately so we may correct our >records. >Please then delete or destroy the original transmission and any >subsequent reply. >Thank you. From zumbor <@t> email.cs.nsw.gov.au Thu Sep 13 20:30:54 2007 From: zumbor <@t> email.cs.nsw.gov.au (Rosalba Zumbo) Date: Thu Sep 13 20:37:20 2007 Subject: [Histonet] Cutting rat brains Message-ID: <002001c7f66e$e4eea8f0$aecbfad0$@cs.nsw.gov.au> I want to cut a frozen rat brain at around 1-3 mm intervals to form blocks. I wish to be able to return to each block at a later date to take further sections if necessary. Can anyone suggest a suitable method or piece of equipment that would aid this task? Thanks From RSRICHMOND <@t> aol.com Thu Sep 13 21:52:14 2007 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Thu Sep 13 21:52:25 2007 Subject: [Histonet] Re: Need help with Brain Tumor FS Message-ID: Theresa asks about technique for cutting brain tumor frozen sections and Tony Henwood replies >>Are your samples received swimming in saline? I would be confident this is your problem.<< Definitely worth a try. With gliomas, crush preparations are extremely useful - take a bit of the tumor and crush it between two slides (not the same as doing an ordinary cytologic imprint, though these are sometimes useful also, particularly with pituitary tumors.) The pathologist needs to know how to interpret all of these modalities. Where I was in my full time job, we'd typically pass the frozen sections around to every pathologist in the house (there were five of us) and we often wound up sending the sections to a nearby consultant anyway. But intraoperative diagnosis of brain tumors is perfectly terrifying, particularly if (like me) the pathologist is inadequately trained in surgical neuropathology. I think that your pathologists are unloading some of this anxiety on you. Believe me, it's hard not to. Bob Richmond Samurai Pathologist Knoxville TN From mmccoy100 <@t> gmail.com Thu Sep 13 22:50:42 2007 From: mmccoy100 <@t> gmail.com (Michelle McCoy) Date: Thu Sep 13 22:50:58 2007 Subject: [Histonet] Gloves for Microtomy Message-ID: <25355ef80709132050u6fb9d57ek96a04fd93c321ea6@mail.gmail.com> Regarding previous postings on this matter-I wonder if the inspectors will push for glove use because they observe microtomy being done around and with the close use of Decal/KOH and other acidic, or toxic chemicals etc. Or observing microtomy being closely tied with coverslipping/stainers with possible cumulative exposure to xylenes and other potential toxic or sensitizing chemicals. Maybe they will assume any neural/ tissue etc could possibly contain CJD. Or possibly they think xylene carryover, formalin chemicals in paraffin/tissue blocks may effect us transdermally?? Any thoughts on this? Michelle From tissuearray <@t> hotmail.com Thu Sep 13 23:04:17 2007 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Thu Sep 13 23:04:34 2007 Subject: [Histonet] New Tissue Micro Array Instrument Message-ID: I thought everyone might like to know there is a new Tissue Microarray instrument on the market. www.arraymold.com Thanks, Thom For free instruction on constructing TMAs go to: www.arrayworkshop.com _________________________________________________________________ Gear up for Halo? 3 with free downloads and an exclusive offer. It?s our way of saying thanks for using Windows Live?. http://gethalo3gear.com?ocid=SeptemberWLHalo3_WLHMTxt_2 From nsnwl <@t> neuro.hfh.edu Thu Sep 13 09:18:03 2007 From: nsnwl <@t> neuro.hfh.edu (Nancy Lemke) Date: Thu Sep 13 23:29:34 2007 Subject: [Histonet]immuno stainers Message-ID: Hi All, I have had a Nemesis for almost 2 years now and would be happy to answer any questions. I run a histology core facility for a group of brain tumor researchers. I stain human only, animal only and human implanted into animal sections. I use the Nemesis in the open mode only, no bar coding. I use primarily Biocare reagents, but few Biocare antibodies. I have done multiple stains on the instrument, including triple and quadruple. I have no complaints about the instrument and find it versatile in every way, which is what I need. All aspects of support are excellent, and I think that Biocare reagents, especially the newer Promark reagents are life savers to people staining xenografts, like myself. I have had past extensive experience with the Biogenex i6000 and the Dako Immunostainer, so I have decent frames of reference. I would be happy to respond to any questions. Nancy Nancy Lemke Research Coordinator Hermelin Brain Tumor Center Henry Ford Hospital Detroit -----Original message----- From: "Sebree Linda A." LSebree@uwhealth.org Date: Thu, 13 Sep 2007 09:11:39 -0400 To: "Christine Tambasco" immrstambo@hotmail.com Subject: RE: [Histonet]immuno stainers > Please post replies to Histonet. > > Linda Sebree, HT(ASCP) > University of Wisconsin Hospital & Clinics > IHC/ISH Laboratory > A4/204-3224 > 600 Highland Ave. > Madison, WI 53792 > (608)265-6596 > FAX: (608)262-7174 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Christine Tambasco > Sent: Wednesday, September 12, 2007 4:26 PM > To: histomike@charter.net; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet]immuno stainers > > > > Anybody have an experiences with the Nemisis from BioCare? > Would > appreciate any feed back. Thanks > > Christine Tambasco, HT (ASCP) > > St Marys Hospital, Amsterdam NY > ______________________________________________________________ > > From: "Mike Schlicht" > To: > Subject: Re: [Histonet] Special Stainers > Date: Wed, 12 Sep 2007 12:51:34 -0700 > >We are currently using the DAKO Artisan for silver stains, and > have > >some problems with uneven staining every now and then, usually > when > >the dispensers are close to being empty, so we stop using the kit > >when there are 5 tests remaining to help reduce some of the > >problems. We had a demo of the Nexus stainer, and found that the > >result were about the same. > >Mike > >San Luis Obispo, CA > > > > > >>Message: 15 > >>Date: Tue, 11 Sep 2007 18:32:37 -0500 > >>From: "Rebecca Johnson" > >>Subject: [Histonet] Special Stainers > >>To: "histonet" > >>Message-ID: <011a01c7f4cc$09fefcc0$7b48f9d8@CHURCH> > >>Content-Type: text/plain; charset="iso-8859-1" > >> > >>What special stainers is everyone using? I now have a Ventana > >>Nexus. Bought in 1999. I know this is old, but it has been > >>maintained well. We have had problems with it several times over > >>the years. Mostly with sliver stains or uneven staining and no > >>staining. I would like to know what others are using and your > >>experiences are. Thanks for all you help. > >>raj > >> > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _________________________________________________________________ > > [1]Kick back and relax with hot games and cool activities at > the > Messenger Café. > > References > > 1. http://g.msn.com/8HMBENUS/2740??PS=47575 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ============================================================================== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================== From mmccoy100 <@t> gmail.com Fri Sep 14 00:01:11 2007 From: mmccoy100 <@t> gmail.com (Michelle McCoy) Date: Fri Sep 14 00:01:25 2007 Subject: [Histonet] States that require license Message-ID: <25355ef80709132201r5b1b0950ya08043f41444700b@mail.gmail.com> Someone in a recent previous post had stated that they thought only one state required state licensing-I know NY and FL do now. I read in an article about NY state licensing http://www.medscape.com/viewarticle/542005 law this excerpt: *With one exception (WV), all states requiring licensure (CA, FL, GA, HI, LA, MT, NV, ND, PR, RI, TN) recognize the American Society for Clinical Pathology (ASCP) as an approved certifying agency.* *Commissioner's regulations require candidates to pass a generalist examination for CLT and CT. With the exception of the grandparenting period (through September 1, 2008) specialists without generalist certification will be required to take a generalist examination to qualify for licensure.* ** -I am hoping to find out if this law applies to Histology as well -If this is an up to date list of the states that require it, if so -If grandfathered techs in these states had to take ascp or if they took a different state test to get the state license. -Is there any site anyone knows about that keeps up to date information on what all the different states are doing regarding licensure requirements in our field other than sifting through the archives here at histonet? Thanks for any input- Michelle From meryl50 <@t> hotmail.com Fri Sep 14 01:02:56 2007 From: meryl50 <@t> hotmail.com (Meryl Roberts) Date: Fri Sep 14 01:03:09 2007 Subject: [Histonet] RE: Histotech Vacancies Message-ID: Have you ever thought about sponsoring a foreign applicant? I ask this because I am a UK resident, and am currently trying to find employment in the USA. I have had alot of interest, due to my experience and qualifications however I find that people are put off when they realise they have to sponsor me, as the process can be quite lengthy. _________________________________________________________________ The next generation of Hotmail is here! http://www.newhotmail.co.uk From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Sep 14 02:29:33 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Sep 14 02:29:50 2007 Subject: [Histonet] Weigert myelin stain procedure Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222ECDC@wahtntex2.waht.swest.nhs.uk> Add 1% calcium chloride to 10% formalin and neutralise with suspended calcium carbonate. Embed tissue in 25% gelatine then cool in a fridge and then harden 1 day in 1% calcium chloride, 1% cadmium chloride, 10% formalin solution- cut sections 15u, attach to slides (by floating onto previously coated slides with 2.5% gelatine), drain then dry. Expose to concentrated formalin to harden gelatine, and put back into calcium, cadmium, formalin solution until needed. Wash slides 3 min in water. Place in cold 5% potassium dichromate in a Coplin jar then place in a 60 degree oven for 48 hours; allow jar to cool. Wash slides in water(dist) then stain in modified Kultschitzky's haematoxylin (haematoxylin 1g, dist water 98 ml, sodium iodate 0.2 g, glacial acetic acid 2ml) at 37 degrees for 5 hours. The haematoxylin forms a resistant black lake with the chromium held by the lipids. Differentiate for 15 hours in Weigert's 1% borax, 2.5% potassium ferrocyanide; only 8 hours for Golgi substance. Wash 5 min in water mount in glycerol gelatine. Cephalin and sphingomyelin are stained black, whilst cerebrosides are grey. If memory serves sections fall off and you eventually lose the will to live but if it works then it's rather pretty. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; The world breaks everyone, and afterward, some are strong at the broken places. --Ernest Hemingway This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From ree3 <@t> leicester.ac.uk Fri Sep 14 03:39:53 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Fri Sep 14 03:40:20 2007 Subject: [Histonet] IHC and state requirements In-Reply-To: <001d01c7f620$ffc5adc0$0202a8c0@yourxhtr8hvc4p> References: <001d01c7f620$ffc5adc0$0202a8c0@yourxhtr8hvc4p> Message-ID: Or what a solution is!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: 13 September 2007 17:13 To: Rene J Buesa; Laurie Colbert; Michelle McCoy; histonet@lists.utsouthwestern.edu Cc: erica.klein@providence.org Subject: Re: [Histonet] IHC and state requirements that's scary. A lab manager who doesn't know what a dilution is. JTT ----- Original Message ----- From: "Rene J Buesa" To: "Laurie Colbert" ; "Michelle McCoy" ; Cc: Sent: Thursday, September 13, 2007 10:48 AM Subject: RE: [Histonet] IHC and state requirements > Probably that lab manager does not know what a dilution is. Anybody > that has studied basic physics, or chemistry know what a dilution rate is. > Unless the person you are referring to is a PhD on "the ocult" or on > arts or the religious field, s/he will know what a dilution is. > Ren? J. > > Laurie Colbert wrote: > I am asking this question for someone from another lab. They recently > got a new lab manager who stated that the person doing IHC's, who is > not licensed as either an HT or HTL but is a Ph.D, is not qualified to > be performing IHC's because it involves the dilution of antibodies. > Evidently, she is comparing this "requirement" which is applied to Med > Techs to that of HT's. > So, I know in the state of California (where we are), there is no > state licensure required for HT's, but is there any requirement that > applies specifically for the dilution of antibodies - in California or > elsewhere??? > > Laurie Colbert > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of > Michelle McCoy > Sent: Wednesday, September 12, 2007 2:31 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] IHC and state requirements > > > I am a Florida licensed HT (ASCP). I'm planning to apply for work out > of the state and have seen jobs titled "Histotechnologist" and was > curious if I am eligible to apply for jobs with this title --if this > term is only applied for HTL credential holders. Or if it is applied > to anyone who has histotechnology experience (I believe someone had > mentioned you dont have to have HT or HTL credentials to work in the > field in many states). Some job listings state IHC duties and I'm > guessing I can perform them as an HT > (ASCP) if I have experience or they are willing to train in IHC > (unless otherwise indicated that they are only looking for HTL > candidates) Thanks for any clarification on this. > > Michelle > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Shape Yahoo! in your own image. Join our Network Research Panel today! > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Fri Sep 14 03:41:40 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Fri Sep 14 03:42:00 2007 Subject: [Histonet] RE: IHC and State requirements In-Reply-To: <002e01c7f621$75ab1b10$0202a8c0@yourxhtr8hvc4p> References: <002e01c7f621$75ab1b10$0202a8c0@yourxhtr8hvc4p> Message-ID: Experiences?,or was it your toe, Joe?. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: 13 September 2007 17:17 To: Rene J Buesa; Patricia Adams; Bernice Frederick; HistoNet Subject: Re: [Histonet] RE: IHC and State requirements to add to Rene's statement. My degrees, titles and experiences got my foot in the door, but it was my experiences that kept me there. JTT ----- Original Message ----- From: "Rene J Buesa" To: "Patricia Adams" ; "Bernice Frederick" ; "HistoNet" Sent: Thursday, September 13, 2007 10:56 AM Subject: RE: [Histonet] RE: IHC and State requirements > That is exactly my contention in an article I just finished about wages in > histology. > When you apply to a job, or negotiate your salary, you are not applying > as part of a group or class, you are negotiating your personal salary and > your experience and abilities are personal, particular, independent of > that of others, with or without a title. > From all the people I have supervised I have had the best amongst those > with lower academic levels, and, viceversa! > The title and the studies qualify you for a title, but does not > habilitate you for the tasks! > Ren? J. > > Patricia Adams wrote: > I am a HT, but have been doing IHC since it was > manual, doing up to 15 different antibodies at a > time and kept it all straight in my head. I am > not sure that having an HLT would have helped > with that. So where do some of us "older" techs > fit into the scheme of things. How do we let the > people hiring know that even though we do not > have the title, we know what we are doing? > Patricia > --- Bernice Frederick > wrote: > >> Hazel, >> I agree with you- I know people who are >> grandfathered and took the exam. >> I've met one person who helped create the HTL >> certification requirements. >> I'm just an HTL but do agree that some have the >> HTL but only know basic >> histo. I know of some institutions where an HTL >> is preferred when it comes >> to IHC (based on the BS). My pet peeve is >> post-docs that think they know >> everything but can't comprehend histo and >> thinek they can do microtomy. We >> require certification for those people that are >> cutting blocks that are >> human cancer trial specimens (a long story) >> Bernice >> >> Bernice Frederick HTL (ASCP) >> Northwestern University >> Pathology Core Facility >> 710 N Fairbanks Court >> Olson 8-421 >> Chicago,IL 60611 >> 312-503-3723 >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> > [mailto:histonet-bounces@lists.utsouthwestern.edu] >> On Behalf Of Horn, Hazel >> V >> Sent: Thursday, September 13, 2007 9:54 AM >> To: Kim Tournear; >> histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] RE: IHC and State >> requirements >> >> Being grandfathered in did not mean they just >> gave you the title of HTL. >> The grandfathered HTL's had to pass the tests >> just like anyone else! >> >> Hazel Horn >> Hazel Horn, HT/HTL (ASCP) >> Supervisor of Histology >> Arkansas Children's Hospital >> 800 Marshall Slot 820 >> Little Rock, AR 72202 >> >> phone 501.364.4240 >> fax 501.364.3912 >> >> visit us on the web at: www.archildrens.org >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> > [mailto:histonet-bounces@lists.utsouthwestern.edu] >> On Behalf Of Kim >> Tournear >> Sent: Thursday, September 13, 2007 9:27 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] RE: IHC and State >> requirements >> >> Hi Michelle, >> A lot employers will except either HT or HTL. >> And of course, how much >> experience you have under your belt is also a >> big consideration. I >> think a lot of employers are finding out just >> how hard it is to find >> techs (registered or not) that can do the job >> and do it well, although >> there are a few states with their own state >> requirements which would >> require the tech to be ASCP certified either >> way. >> >> I have found that being an HTL vs HT doesn't >> necessarily mean that one >> or the other can do the job better...I know a >> few HTL's that were >> grandfathered in and have done only a handful >> IHCs in their whole >> career, can't trouble shoot anything, and >> produce poor quality slides >> and yet they hold the title of an HTL and the >> same goes for the >> HTs...(makes you wonder how they keep a >> job)...LOL....and I have taught >> and worked with students right out of college >> programs that were >> absolutely awesome and knew their stuff....go >> figure.....I say go for >> it.....Good Luck.... >> >> >> Kim Tournear, HT (ASCP), QIHC ( ASCP) >> Specialists in Dermatology >> Histology/Mohs Supervisor >> Tucson, AZ >> >> >> >> >> --------------------------------- >> Boardwalk for $500? In 2007? Ha! >> Play Monopoly Here and Now (it's updated for >> today's economy) at Yahoo! >> Games. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > ---------------------------------------------------------------------------- >> -- >> The information contained in this message may >> be privileged and confidential >> and protected from disclosure. If the reader of >> this message is not the >> intended recipient, or an employee or agent >> responsible for delivering this >> message to the intended recipient, you are >> hereby notified that any >> dissemination, distribution or copying of this >> communication is strictly >> prohibited. If you have received this >> communication in error, please notify >> us immediately by replying to the message and >> deleting it from your >> computer. >> Thank you. >> > ============================================================================ >> == >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > Patricia Adams > ----- > Fight back spam! Download the Blue Frog. > http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D > > > > ____________________________________________________________________________________ > Yahoo! oneSearch: Finally, mobile search > that gives answers, not web links. > http://mobile.yahoo.com/mobileweb/onesearch?refer=1ONXIC > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Be a better Heartthrob. Get better relationship answers from someone who > knows. > Yahoo! Answers - Check it out. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From simmonsca <@t> upmc.edu Fri Sep 14 04:10:26 2007 From: simmonsca <@t> upmc.edu (Simmons, Christopher) Date: Fri Sep 14 04:10:42 2007 Subject: [Histonet] Histotech vacancies In-Reply-To: <24A4826E8EF0964D86BC5317306F58A502FEBB8586@mmc-mail.ad.mhsil.com> References: <24A4826E8EF0964D86BC5317306F58A502FEBB8586@mmc-mail.ad.mhsil.com> Message-ID: <88D6F512ADC2CB428B90845D2D1388E303A5BB5F@1upmc-msx6.acct.upmchs.net> Our hospital has created a histo school to try to alleviate the shortage, we are also attempting to get the pay scale changed to attract new talent into our lab. Chris Simmons Lead Tech PUH Histology 412.647.7660 desk 13242 pager -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Thursday, September 13, 2007 12:15 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Histotech vacancies Like a lot of hospitals we have been unable to find qualified applicants for a histotech position. We are at least exploring other options. One of the options include hiring someone with a biological background and enrolling them in an on-line histotech training program. After completion of the program the applicant would take the certification examination. There are a few schools out there that have full time programs but many individuals are not in a position to take six months off to attend them without receiving a weekly paycheck. In addition my experience has been that these schools only take a small amount of applicants each session. So with more positions than students has anyone else come up with a creative way to train and hire histotechs and any suggestions to on-line programs that have been worthwhile. Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Fri Sep 14 05:45:37 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 14 05:45:59 2007 Subject: [Histonet] Surgical specimens In-Reply-To: <41E16A15CE78374EA45B57E0F94339B802C58797@ORLEV01.hca.corpad.net> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F3B22@sjhaexc02.sjha.org> We pick ours up Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Vacca Jessica Sent: Thursday, September 13, 2007 4:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Surgical specimens Those of you that are hospital based........... Does surgery bring their surgical specimens to you or do you go to surgery to pick them up? We currently go to surgery, but since we are so short staffed...Did I mention we have a FT HT/HTL position available???? I'm going to present to my Lab director to see if I can get a "circulator" to deliver the specimens? Just wanted to get a consensus. Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Drive Brandon,FL 33511 813-571-5193 813-571-5169 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From rjr6 <@t> psu.edu Fri Sep 14 07:12:51 2007 From: rjr6 <@t> psu.edu (Roberta Horner) Date: Fri Sep 14 07:13:04 2007 Subject: [Histonet] a funny for Friday Message-ID: I received this joke from a friend and thought it would entertaining to end the week. Roberta A guy walks into a bar, sits down, and asks, "Bartender, got any specials today?" Bartender answers, "Yes, as a matter of fact we have a new drink, invented by a gynecologist patron of ours. It's a mix of Pabst Blue Ribbon Beer and Smirnoff Vodka." The guy asks, "Good grief, what do you call that?" The bartender replied, "It's a 'Pabst Smir'". From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Sep 14 07:16:36 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Sep 14 07:16:45 2007 Subject: [Histonet] a funny for Friday Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222ECEA@wahtntex2.waht.swest.nhs.uk> The joke is Liquid Based? Yes? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; The world breaks everyone, and afterward, some are strong at the broken places. --Ernest Hemingway This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From rjbuesa <@t> yahoo.com Fri Sep 14 07:18:05 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Sep 14 07:18:17 2007 Subject: [Histonet] Surgical specimens In-Reply-To: <41E16A15CE78374EA45B57E0F94339B802C58797@ORLEV01.hca.corpad.net> Message-ID: <881624.72097.qm@web61220.mail.yahoo.com> We always used a lab aide to pick-up specimens. We never wanted to depend on others to deliver the specimens, because there could be problems with the delivery hours fitting into our schedules. Ren? J. Vacca Jessica wrote: Those of you that are hospital based........... Does surgery bring their surgical specimens to you or do you go to surgery to pick them up? We currently go to surgery, but since we are so short staffed...Did I mention we have a FT HT/HTL position available???? I'm going to present to my Lab director to see if I can get a "circulator" to deliver the specimens? Just wanted to get a consensus. Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Drive Brandon,FL 33511 813-571-5193 813-571-5169 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! oneSearch: Finally, mobile search that gives answers, not web links. From b-frederick <@t> northwestern.edu Fri Sep 14 07:34:41 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Sep 14 07:34:52 2007 Subject: [Histonet] Surgical specimens In-Reply-To: <004001c7f649$4a2bd2e0$6701a8c0@CHERYLSLAPTOP> Message-ID: <000501c7f6cb$a2abf1c0$d00f7ca5@lurie.northwestern.edu> Well, Having worked in a small hospital where we ran to surgery (it was one floor up)to a large hospital- larger hospitals may have a circuator to pick up and drop off. At Northwestern the gross room is next to surgery so the OR places them in the fridge themselves. Same for Advocate Christ Hospital. The grossed tissue is then transported by the residents and or gross room techs to the histology lab for processing. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl R. Kerry Sent: Thursday, September 13, 2007 4:02 PM To: 'Vacca Jessica'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Surgical specimens Hi Jessica- I've been in facilities that handled it either way--and some that did both depending on the staffing situation and sometimes the nurse staff ran stuff by on their way home. As long as there was consistency in being checked before being checked in, it went well. Orienting the new folks to the procedure was the most important part of the deal and having a laminated 'check list' sort of reminder with an example posted at the log did wonders to keep the problems in ID and mislabeling under control. Having the runner (circulator, tech, nurse) sign stuff in (keeping them responsible) was an important part of the process. My two-cents! Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc 281.852.9457 Staffing the AP Lab - One great tech at a time! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vacca Jessica Sent: Thursday, September 13, 2007 3:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Surgical specimens Those of you that are hospital based........... Does surgery bring their surgical specimens to you or do you go to surgery to pick them up? We currently go to surgery, but since we are so short staffed...Did I mention we have a FT HT/HTL position available???? I'm going to present to my Lab director to see if I can get a "circulator" to deliver the specimens? Just wanted to get a consensus. Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Drive Brandon,FL 33511 813-571-5193 813-571-5169 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Fri Sep 14 07:42:52 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Sep 14 07:43:02 2007 Subject: [Histonet] tissue of the week - shrew mandibles In-Reply-To: <6.2.3.4.1.20070913164500.01f7c728@algranth.inbox.email.arizona.edu> Message-ID: <001201c7f6cc$c7461a50$d00f7ca5@lurie.northwestern.edu> Well tamed,I should think! Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea Grantham Sent: Thursday, September 13, 2007 6:47 PM To: Joe Nocito; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] tissue of the week - shrew mandibles JTT, They have been tamed - in fact they are sleeping nicely over the weekend in cassettes waiting for their dunk into decal on Monday morning. Andi At 04:10 PM 9/13/2007, Joe Nocito wrote: >Andi, >would that taming of the shrew!!! I'm such the knee slapper, I kill myself. >Back to your question, no, I don't have a procedure. > >JTT >----- Original Message ----- From: "Andrea Grantham" >To: >Sent: Thursday, September 13, 2007 5:21 PM >Subject: [Histonet] tissue of the week - shrew mandibles > > >>Does anybody have a protocol for decalcification and processing of >>shrew mandibles? >>They are just a little over a centimeter in length and not very >>thick so I don't think the decaling would be very long. >>Haven't done this type of tissue before so I'm wondering if anybody >>out there has some hints for sectioning. The person who brought >>them said they could be hard to cut. >>Thanks. >>Andi >>..................................................................... >>: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : >>: Sr. Research Specialist University of Arizona : >>: (office: AHSC 4212) P.O. Box 245044 : >>: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : >>: (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : >>:...................................................................: >> http://www.cba.arizona.edu/histology-lab.html >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ROrr <@t> enh.org Fri Sep 14 07:59:50 2007 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Fri Sep 14 08:00:04 2007 Subject: [Histonet] My experiences with HT vacancies Message-ID: Cripes I wrote a BOOK! I don't have enough to do today. Actually I do, I'm just avoiding it. So if anyone is really interested enough to read this through, and there's anything I can do to help, just email me. Our experience with online training has been very good. First of all I attended one of Peggy Wenk's seminars on starting your own training program, it was very informative and Peggy was very helpful in the follow up with my questions and issues. THANKS PEGGY!!!! We had to get NCCLS accreditation so that we could affiliate with the college that was offering the online classes. We already had a set up in place, since our laboratory also has MT training school. We worked closely with the Harford College to attain this. We had our HR change some job descriptions around so that we had some "flex" positions. So if we had openings and we had a qualified tech to fill it, they could be hired as such. We were able to flex two positions so that if there wasn't a tech available we could fill it with a student. When this occurred we designated these positions as Histology Trainee. These students were hired as full time staff and their tuition was paid via our tuition re-imbursement program. (We get up to 5k/year here). We were able to arrange that the students sign a commitment of 2 years after they finished the online course. They were expected to pass the HT exam within a period of time (I can't remember what we decided). IF they chose to leave employment before the 2 years, all they had to do was refund their tuition. It has been a great experience for our lab and rejuvenated our morale. We have two bright kids who transferred over from clerical positions. One of them had to take a couple of night school classes to fulfill the requirements for the college, but we couldn't be more pleased with the results. We have a staff of more senior (ok ok OLD! Me included) who have a ton of experience we're just waiting to share with these students. I think for my staff, they have a great sense of satisfaction and I have been quite surprised at their ability to teach, something they haven't had a chance to do. I am very glad the staff here has a commitment to this as well...I think you need their "buy in" in order for these students to get the best training. I do monitor their progress and review all their assignments. They complete modules and I check it over and then add my additional questions for the students. I am working on a more formal Syllabus for us to use and am learning as the students and I progress. They are learning how to be histo techs and I'm learning how to teach them. For example, they had an assignment about the different kinds of microscopes. I asked additional questions around the use of the microscopes, what type of special stains required a fluorescent scope, what's the difference between resolution and magnification, what is refractive index, plastic tape versus glass coverslip...and I had them identify the parts of the 'scope...I ended up with about 30- 50 more in depth questions to complement their training. We have a fluorescent scope, dissecting scope,etc, and an ancient GIANT EM scope...so we went on a "field trip" to look at the scopes and use them....They have a contact person at the college and they keep in touch with her on a weekly basis. They have assignments to finish on their own pace, but I keep them on a steady schedule so they will finish everything in the 10 month period. We give them time during the day to do assignments, but they do spend the greater part of their day working with the Tech's. Whew! HAPPY FRIDAY! Becky Orr CLA,HT(ASCP)QIHC Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 From Luis.Chiriboga <@t> med.nyu.edu Fri Sep 14 09:21:25 2007 From: Luis.Chiriboga <@t> med.nyu.edu (Luis Chiriboga) Date: Fri Sep 14 08:15:37 2007 Subject: [Histonet] States that require license In-Reply-To: <25355ef80709132201r5b1b0950ya08043f41444700b@mail.gmail.com> Message-ID: Hi everyone I'm getting into this thread a bit late as I was not receiving Histonet messages for the last couple of weeks. A colleague mentioned this thread and I thought I should follow-up. I apologize if this information has already been posted. Michelle, I'm not sure what your original post was but I understand there was some question regarding licensing requirements and certification in different states. I can only speak for New York State.... You are correct, NYS now requires any individual working in a clinical laboratory to be licensed. This law went into effect last September 06 with a grand fathering provision that just expired this September. I think its important to recognize that there is a significant difference between "licensing" and "certification". "Licensing" is performed by a government organization while "certification" can be performed by almost any organization. Obviously, the value of a certification is based on the veracity of the organization providing it. While it is also true that many other states view ASCP as a valid certifying agency and, ASCP certification as a measure of competency, the NYS education department (NYSED) has chosen not to accept ASCP certification as a means of credentialing for grand fathering or as a means to establish competency in the absence or presence of an examination for any laboratory discipline. However, I believe that NYSED has selected ASCP to create and administer a "generalist" exam based on the educational curriculum mandated by the NYSED and the Clinical Laboratory Technology Practice Act. As the law currently stands in NYS, anyone who was not grad fathered will have to meet the educational requirements AND take the licensing examination in order to be employed in NYS (for detail info on the curriculum material, please see article 165 of the education law http://www.op.nysed.gov/clp.htm). In NYS, this law applies to all clinical laboratory personnel, including histologist. NYS does not recognize nor require ASCP certification for licensing. For those of you in other states that are considering proposing a licensing law (I think I saw a message thread from a few on this), I suggest that you take a look at the NYS law...... I hope this helps. If you have any questions, please feel free to respond. Luis Luis Chiriboga PhD., HT (ASCP) QIHC New York University School of Medicine NYU Cancer Institute IHC Core Facility Bellevue Hospital Center Department of Pathology 4W27 27th Street & First Avenue New York, N.Y. 10016 V (212)562-4667 F (212)263-2041 Vice President New York State Histotechnological Society http://www.nyhisto.org/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Michelle McCoy Sent: Friday, September 14, 2007 12:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] States that require license Someone in a recent previous post had stated that they thought only one state required state licensing-I know NY and FL do now. I read in an article about NY state licensing http://www.medscape.com/viewarticle/542005 law this excerpt: *With one exception (WV), all states requiring licensure (CA, FL, GA, HI, LA, MT, NV, ND, PR, RI, TN) recognize the American Society for Clinical Pathology (ASCP) as an approved certifying agency.* *Commissioner's regulations require candidates to pass a generalist examination for CLT and CT. With the exception of the grandparenting period (through September 1, 2008) specialists without generalist certification will be required to take a generalist examination to qualify for licensure.* ** -I am hoping to find out if this law applies to Histology as well -If this is an up to date list of the states that require it, if so -If grandfathered techs in these states had to take ascp or if they took a different state test to get the state license. -Is there any site anyone knows about that keeps up to date information on what all the different states are doing regarding licensure requirements in our field other than sifting through the archives here at histonet? Thanks for any input- Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Fri Sep 14 08:29:17 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 14 08:41:07 2007 Subject: [Histonet] a funny for Friday Message-ID: <407F05A128805F4C879A33DBA32E618E01895051@TRFT-EX01.xRothGen.nhs.uk> A guy walks into a bar with a lump of asphalt under his arm, sits down, and asks, "Bartender, gimme a beer, oh, and one for the road". Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roberta Horner Sent: 14 September 2007 13:13 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] a funny for Friday I received this joke from a friend and thought it would entertaining to end the week. Roberta A guy walks into a bar, sits down, and asks, "Bartender, got any specials today?" Bartender answers, "Yes, as a matter of fact we have a new drink, invented by a gynecologist patron of ours. It's a mix of Pabst Blue Ribbon Beer and Smirnoff Vodka." The guy asks, "Good grief, what do you call that?" The bartender replied, "It's a 'Pabst Smir'". _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Sep 14 08:46:41 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Sep 14 08:46:50 2007 Subject: [Histonet] a funny for Friday Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222ECEF@wahtntex2.waht.swest.nhs.uk> A woman goes into a bar and asks for a "double entendre". So the bartender gave her one. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; The world breaks everyone, and afterward, some are strong at the broken places. --Ernest Hemingway This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From HornHV <@t> archildrens.org Fri Sep 14 08:50:00 2007 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Sep 14 08:50:21 2007 Subject: [Histonet] Surgical specimens In-Reply-To: <000501c7f6cb$a2abf1c0$d00f7ca5@lurie.northwestern.edu> References: <004001c7f649$4a2bd2e0$6701a8c0@CHERYLSLAPTOP> <000501c7f6cb$a2abf1c0$d00f7ca5@lurie.northwestern.edu> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB733F@EMAIL.archildrens.org> We have someone from surgery to bring our specimens to the lab. They deliver several times a day. The clinics bring their specimens to us as well. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Friday, September 14, 2007 7:35 AM To: 'Cheryl R. Kerry'; 'Vacca Jessica'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Surgical specimens Well, Having worked in a small hospital where we ran to surgery (it was one floor up)to a large hospital- larger hospitals may have a circuator to pick up and drop off. At Northwestern the gross room is next to surgery so the OR places them in the fridge themselves. Same for Advocate Christ Hospital. The grossed tissue is then transported by the residents and or gross room techs to the histology lab for processing. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl R. Kerry Sent: Thursday, September 13, 2007 4:02 PM To: 'Vacca Jessica'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Surgical specimens Hi Jessica- I've been in facilities that handled it either way--and some that did both depending on the staffing situation and sometimes the nurse staff ran stuff by on their way home. As long as there was consistency in being checked before being checked in, it went well. Orienting the new folks to the procedure was the most important part of the deal and having a laminated 'check list' sort of reminder with an example posted at the log did wonders to keep the problems in ID and mislabeling under control. Having the runner (circulator, tech, nurse) sign stuff in (keeping them responsible) was an important part of the process. My two-cents! Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc 281.852.9457 Staffing the AP Lab - One great tech at a time! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vacca Jessica Sent: Thursday, September 13, 2007 3:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Surgical specimens Those of you that are hospital based........... Does surgery bring their surgical specimens to you or do you go to surgery to pick them up? We currently go to surgery, but since we are so short staffed...Did I mention we have a FT HT/HTL position available???? I'm going to present to my Lab director to see if I can get a "circulator" to deliver the specimens? Just wanted to get a consensus. Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Drive Brandon,FL 33511 813-571-5193 813-571-5169 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From Terry.Marshall <@t> rothgen.nhs.uk Fri Sep 14 09:00:44 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 14 09:01:12 2007 Subject: [Histonet] a funny for Friday Message-ID: <407F05A128805F4C879A33DBA32E618E01895054@TRFT-EX01.xRothGen.nhs.uk> Now I really like that one! Terry -----Original Message----- From: Kemlo Rogerson [mailto:Kemlo.Rogerson@waht.swest.nhs.uk] Sent: 14 September 2007 14:47 To: Marshall Terry Dr, Consultant Histopathologist; Roberta Horner; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] a funny for Friday A woman goes into a bar and asks for a "double entendre". So the bartender gave her one. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; The world breaks everyone, and afterward, some are strong at the broken places. --Ernest Hemingway This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From yvan_lindekens <@t> yahoo.com Fri Sep 14 09:06:07 2007 From: yvan_lindekens <@t> yahoo.com (yvan lindekens) Date: Fri Sep 14 09:06:18 2007 Subject: [Histonet] Heidenhain's AZAN modification of mallory's triple stain: protocol, hand staining Message-ID: <441750.94444.qm@web30911.mail.mud.yahoo.com> Acronym of AZocarmine-ANilin blue. Martin Heidenhain really loved acronyms: AZAN, SUSA... (By hand) Fixation: any, but best results after fixation in fixatives containing corrosive sublimate. - Bring sections to water - Stain in azocarmine G solution, warmed up to about 56?C for 10 - 15 min or in azocarmine B @ RT for an hour(1) - brief rince in distilled water - differentiation in anilin-ETOH untill only nuclei, erytrocytes and musle (the latter depending on fixation) are stained a vivid red (2) - thorough rinse in ETOH-acetic acid to stop differentiation and to remove anilin (3) - Mordant in phosphotungstic acid solution for 1-3hrs (4) - brief rince in distilled water - Stain in anilin blue-orange G solution for 1-3hrs(5) - rinse in distilled water - dehydrate/differentiate the counterstan in ETOH/IPA. - Clear and mount Results: collagen and reticular connective tissue sharp blue, nuclei: vivid red, muscle orange to red (depending on fixation), erytrocytes: red, glia fibers: red. (1) Azocarmine staining solution Dissolve 0.1gm azocarmine G in 100 ml boiling distilled water. Cool down, add 1ml glacial acetic acid OR: Dissolve 0.1gm azocarmine B in 100 ml distilled water and add 1ml of glacial acetic acid (2) Anilin-ETOH differentiation solution ETOH 96%: 1000ml Anilin: 1ml If anilin isn't available: use crude wood spirit as the differentiator OR: ETOH 96%: 1000ml pyridin: 1ml (3) ETOH-acetic acid rinsing fluid ETOH 96%: 1000ml Glacial acetic acid: 10ml (4) Mordanting solution Distilled water: 1000ml Phosphotungstic acid: 50gm (5) Anilin blue-orange G staining solution Distilled water: 1000ml Anilin blue: 5gm Orange G: 20gm Glacial acetic acid: 80ml Bring to the boil and cool down after dissolution of the dyes and filter. There are still a few other languages besides English. Less important, I know, but nevertheless: they exist. Perhaps it would be a good idea to import some German, French, Italian, Spanish slide prep manuals into the States... ____________________________________________________________________________________ Moody friends. Drama queens. Your life? Nope! - their life, your story. Play Sims Stories at Yahoo! Games. http://sims.yahoo.com/ From audrabilbo <@t> yahoo.com Fri Sep 14 09:21:01 2007 From: audrabilbo <@t> yahoo.com (audra bilbo) Date: Fri Sep 14 09:21:13 2007 Subject: [Histonet] HPV testing Message-ID: <939852.53307.qm@web32015.mail.mud.yahoo.com> What is the best method for Cytology HPV testing? We run both Thin Prep and SurePath specimens. Audra Bilbo Texarkana, TX ____________________________________________________________________________________ Luggage? GPS? Comic books? Check out fitting gifts for grads at Yahoo! Search http://search.yahoo.com/search?fr=oni_on_mail&p=graduation+gifts&cs=bz From timothy.macatee <@t> med.nyu.edu Fri Sep 14 09:55:02 2007 From: timothy.macatee <@t> med.nyu.edu (Timothy Macatee) Date: Fri Sep 14 09:56:36 2007 Subject: [Histonet] a funny for Friday In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBF0222ECEF@wahtntex2.waht.swest.nhs.uk> Message-ID: A priest, a rabbi and the Dalai Lama walk into a bar. The bartender says, "Is this some kind of a joke?" On 9/14/07 9:46 AM, "Kemlo Rogerson" wrote: > A woman goes into a bar and asks for a "double entendre". So the > bartender gave her one. > > Kemlo Rogerson > Pathology Manager > DD 01934 647057 or extension 3311 > Mob 07749 754194; Pager 07659 597107; > > > The world breaks everyone, and afterward, some are strong at the broken > places. --Ernest Hemingway > > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. > Please inform me that this message has gone astray before deleting it. > Thank you for your co-operation > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Tim Macatee Research Histology Core New York University School of Medicine 550 First Ave. Department of Pathology. Medical Science Building - Room 504 New York, N.Y. 10016 (212) 263-3888 From gcallis <@t> montana.edu Fri Sep 14 09:58:12 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 14 09:57:36 2007 Subject: [Histonet] tissue of the week - shrew mandibles In-Reply-To: <6.2.3.4.1.20070913151703.01f92b38@algranth.inbox.email.ari zona.edu> References: <6.2.3.4.1.20070913151703.01f92b38@algranth.inbox.email.arizona.edu> Message-ID: <6.0.0.22.1.20070914082952.01b30998@gemini.msu.montana.edu> Good morning Andi, Probably not any more difficult than doing a whole mouse or rat skull other than the time needed to complete decalcification. We decalcify in 10% formic acid but do use a decalcification endpoint test to make sure teeth and everything else are decalcified. They probably will not take very long, so you may need to interupt the decalcification near endpoint by placing in NBF overnight then resuming decalcification the next day. You could use a mixture of 4% HCl/8% formic acid which is a bit faster than the 10% formic acid, but don't let bones sit in this overnight. If you want, I have a weight loss/weight gain method for testing, which requires a balance the weighs in mg. If you are worried about over-exposure to acid i.e. over decalcification, and have the time on your side to have a good preparation, then 14% tetrasodium EDTA with pH adjusted down to 7.4 will work also. That way you don't need to worry so much about timing. We weigh out 14 g tetrasodium EDTA, dissolve in 80 mls distilled water or Dulbeccos PBS, and adjust the pH down with glacial acetic acid, then bring final volume to 100 mls. This is a method from Dr. Webb Jee, an orthopedic bone expert. We have preference for the tetrasodium EDTA since it goes into solution so easily and at that wonderfully high concentration versus EDTA or disodium EDTA where these are only soluble to approx 10% with the help of heat and/or addition of sodium hydroxide. The problem with tetrasodium EDTA is the beginning pH is above 9, and alkaline sensitive protein bonds will be affected by the high pH. We do the pH adjustment with an electrode in the solution and titrate it to pH 7.4 as this will take a fairly large amount of acetic acid. I have adjusted the pH to 7.6 which is also the pH of our TBS buffer, and achieved excellent results. EDTA decalcifies as a function of pH and at pH 7 the decalcification is slower since the molecule is not fully protonated until pH 8. However pH 8 is pushing the limits for alkaline sensitive protein bond related problems so we keep the pH around 7.4 - 7.6. I am going to attach the weight gain/weight loss method to you privately - this works with acids and EDTA methods, and was originally used for testing nitric acid decalcification endpoint. If you have a FAXITRON, your endpoint testing would be simple. EDTA is a pain to test chemically. When processing, be sure to add some time onto paraffin infiltration with these tiny dense bones plus teeth? As for sectioning, it will be easier for bone if you use a harder paraffin i.e. Tissue Prep 2 from Fisher (whatever), but other paraffin work as long as infiltration is adequate. We use high profile blades for sectioning and less chance of chatter, less stability we experience with bone microtomy using low profile blades. Orientation will be important depending on what they want. A good soak after trimming will help, often with RT water, then go to ice water but don't oversoak. Plus charge slides work well, drain after pickup, and dry sections FLAT at 37 to 40C overnight, although we find several days works better to retain sections on slides. Good luck Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 At 04:21 PM 9/13/2007, you wrote: >Does anybody have a protocol for decalcification and processing of shrew >mandibles? >They are just a little over a centimeter in length and not very thick so I >don't think the decaling would be very long. >Haven't done this type of tissue before so I'm wondering if anybody out >there has some hints for sectioning. The person who brought them said they >could be hard to cut. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From gu.lang <@t> gmx.at Fri Sep 14 09:59:39 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Sep 14 09:59:52 2007 Subject: AW: [Histonet] Surgical specimens In-Reply-To: <41E16A15CE78374EA45B57E0F94339B802C58797@ORLEV01.hca.corpad.net> Message-ID: <000001c7f6df$e0714cd0$6412a8c0@dielangs.at> Few years ago there was a hospital service that brought the specimen. Now we are connected with a "railway" with stations in each department. Big specimen are brought up to now. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Vacca Jessica Gesendet: Donnerstag, 13. September 2007 22:40 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Surgical specimens Those of you that are hospital based........... Does surgery bring their surgical specimens to you or do you go to surgery to pick them up? We currently go to surgery, but since we are so short staffed...Did I mention we have a FT HT/HTL position available???? I'm going to present to my Lab director to see if I can get a "circulator" to deliver the specimens? Just wanted to get a consensus. Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Drive Brandon,FL 33511 813-571-5193 813-571-5169 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Fri Sep 14 10:09:07 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 14 10:08:42 2007 Subject: [Histonet] Cutting rat brains In-Reply-To: <002001c7f66e$e4eea8f0$aecbfad0$@cs.nsw.gov.au> References: <002001c7f66e$e4eea8f0$aecbfad0$@cs.nsw.gov.au> Message-ID: <6.0.0.22.1.20070914090457.01b58db8@gemini.msu.montana.edu> You can purchase a brain matrix device, from many places. Try Charles Scouten's website, Google his website with keey word myneurolab. These devices allow you to section rat brains by placing the brain in a form, and then making precise slices, then you can proceed with how you want to orient, etc. You need to make sure the brains are kept flat during snap freezing after the slices are made. There is a way to do this using a liquid nitrogen/petri dish setup. If you wish I will send the mini powerpoint on how to do this. At 07:30 PM 9/13/2007, you wrote: >I want to cut a frozen rat brain at around 1-3 mm intervals to form blocks. >I wish to be able to return to each block at a later date to take further >sections if necessary. Can anyone suggest a suitable method or piece of >equipment that would aid this task? Thanks > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From doug <@t> ppspath.com Fri Sep 14 11:24:42 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Fri Sep 14 10:25:18 2007 Subject: [Histonet] a funny for Friday In-Reply-To: Message-ID: A three-legged dog walks into a bar and says, "I'm lookin' for the man who shot my paw." Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Timothy Macatee Sent: Friday, September 14, 2007 9:55 AM To: Kemlo Rogerson; Marshall Terry Dr, Consultant Histopathologist; Roberta Horner; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] a funny for Friday A priest, a rabbi and the Dalai Lama walk into a bar. The bartender says, "Is this some kind of a joke?" On 9/14/07 9:46 AM, "Kemlo Rogerson" wrote: > A woman goes into a bar and asks for a "double entendre". So the > bartender gave her one. > > Kemlo Rogerson > Pathology Manager > DD 01934 647057 or extension 3311 > Mob 07749 754194; Pager 07659 597107; > > > The world breaks everyone, and afterward, some are strong at the broken > places. --Ernest Hemingway > > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. > Please inform me that this message has gone astray before deleting it. > Thank you for your co-operation > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Tim Macatee Research Histology Core New York University School of Medicine 550 First Ave. Department of Pathology. Medical Science Building - Room 504 New York, N.Y. 10016 (212) 263-3888 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Fri Sep 14 10:30:45 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 14 10:31:09 2007 Subject: [Histonet] a funny for Friday In-Reply-To: Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F3B3F@sjhaexc02.sjha.org> These are all great... thanks for the laughs all morning!! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Douglas D Deltour Sent: Friday, September 14, 2007 12:25 PM To: 'Timothy Macatee'; 'Kemlo Rogerson'; 'Marshall Terry Dr,Consultant Histopathologist'; 'Roberta Horner'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] a funny for Friday A three-legged dog walks into a bar and says, "I'm lookin' for the man who shot my paw." Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Timothy Macatee Sent: Friday, September 14, 2007 9:55 AM To: Kemlo Rogerson; Marshall Terry Dr, Consultant Histopathologist; Roberta Horner; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] a funny for Friday A priest, a rabbi and the Dalai Lama walk into a bar. The bartender says, "Is this some kind of a joke?" On 9/14/07 9:46 AM, "Kemlo Rogerson" wrote: > A woman goes into a bar and asks for a "double entendre". So the > bartender gave her one. > > Kemlo Rogerson > Pathology Manager > DD 01934 647057 or extension 3311 > Mob 07749 754194; Pager 07659 597107; > > > The world breaks everyone, and afterward, some are strong at the broken > places. --Ernest Hemingway > > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. > Please inform me that this message has gone astray before deleting it. > Thank you for your co-operation > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Tim Macatee Research Histology Core New York University School of Medicine 550 First Ave. Department of Pathology. Medical Science Building - Room 504 New York, N.Y. 10016 (212) 263-3888 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From gcallis <@t> montana.edu Fri Sep 14 10:38:03 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 14 10:37:28 2007 Subject: learning to spell Re: [Histonet] Cutting rat brains In-Reply-To: <6.0.0.22.1.20070914090457.01b58db8@gemini.msu.montana.edu> References: <002001c7f66e$e4eea8f0$aecbfad0$@cs.nsw.gov.au> <6.0.0.22.1.20070914090457.01b58db8@gemini.msu.montana.edu> Message-ID: <6.0.0.22.1.20070914093624.01b0a4f0@gemini.msu.montana.edu> Sorry, fingers are responsible for misspelled words - key! TGIF and the Friday joke too. Gayle Callis At 09:09 AM 9/14/2007, you wrote: >You can purchase a brain matrix device, from many places. Try Charles >Scouten's website, Google his website with keey word myneurolab. These >devices allow you to section rat brains by placing the brain in a form, >and then making precise slices, then you can proceed with how you want to >orient, etc. > >You need to make sure the brains are kept flat during snap freezing after >the slices are made. There is a way to do this using a liquid >nitrogen/petri dish setup. If you wish I will send the mini powerpoint on >how to do this. From simmonsca <@t> upmc.edu Fri Sep 14 10:40:26 2007 From: simmonsca <@t> upmc.edu (Simmons, Christopher) Date: Fri Sep 14 10:40:40 2007 Subject: [Histonet] a funny for Friday In-Reply-To: <1UPMC-MSXGW5wSA1Xvp0000e343@1upmc-msxgw5.isdip.upmc.edu> References: <1UPMC-MSXGW5wSA1Xvp0000e343@1upmc-msxgw5.isdip.upmc.edu> Message-ID: <88D6F512ADC2CB428B90845D2D1388E303A5BB6D@1upmc-msx6.acct.upmchs.net> How about some funny movie quotes.... Whats the matter Colonel Sanders?....Chicken?! ---Spaceballs Chris Simmons Lead Technologist UPMC Presbyterian Shadyside Histology 412.647.7660 desk 13242 pager -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Friday, September 14, 2007 12:25 PM To: 'Timothy Macatee'; 'Kemlo Rogerson'; 'Marshall Terry Dr,Consultant Histopathologist'; 'Roberta Horner'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] a funny for Friday A three-legged dog walks into a bar and says, "I'm lookin' for the man who shot my paw." Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Timothy Macatee Sent: Friday, September 14, 2007 9:55 AM To: Kemlo Rogerson; Marshall Terry Dr, Consultant Histopathologist; Roberta Horner; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] a funny for Friday A priest, a rabbi and the Dalai Lama walk into a bar. The bartender says, "Is this some kind of a joke?" On 9/14/07 9:46 AM, "Kemlo Rogerson" wrote: > A woman goes into a bar and asks for a "double entendre". So the > bartender gave her one. > > Kemlo Rogerson > Pathology Manager > DD 01934 647057 or extension 3311 > Mob 07749 754194; Pager 07659 597107; > > > The world breaks everyone, and afterward, some are strong at the broken > places. --Ernest Hemingway > > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. > Please inform me that this message has gone astray before deleting it. > Thank you for your co-operation > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Tim Macatee Research Histology Core New York University School of Medicine 550 First Ave. Department of Pathology. Medical Science Building - Room 504 New York, N.Y. 10016 (212) 263-3888 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Fri Sep 14 10:43:27 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Fri Sep 14 10:43:45 2007 Subject: [Histonet] a funny for Friday In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F3B3F@sjhaexc02.sjha.org> References: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F3B3F@sjhaexc02.sjha.org> Message-ID: Shell I tell you an egg yolk?. All white!. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: 14 September 2007 16:31 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] a funny for Friday These are all great... thanks for the laughs all morning!! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Douglas D Deltour Sent: Friday, September 14, 2007 12:25 PM To: 'Timothy Macatee'; 'Kemlo Rogerson'; 'Marshall Terry Dr,Consultant Histopathologist'; 'Roberta Horner'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] a funny for Friday A three-legged dog walks into a bar and says, "I'm lookin' for the man who shot my paw." Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Timothy Macatee Sent: Friday, September 14, 2007 9:55 AM To: Kemlo Rogerson; Marshall Terry Dr, Consultant Histopathologist; Roberta Horner; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] a funny for Friday A priest, a rabbi and the Dalai Lama walk into a bar. The bartender says, "Is this some kind of a joke?" On 9/14/07 9:46 AM, "Kemlo Rogerson" wrote: > A woman goes into a bar and asks for a "double entendre". So the > bartender gave her one. > > Kemlo Rogerson > Pathology Manager > DD 01934 647057 or extension 3311 > Mob 07749 754194; Pager 07659 597107; > > > The world breaks everyone, and afterward, some are strong at the broken > places. --Ernest Hemingway > > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. > Please inform me that this message has gone astray before deleting it. > Thank you for your co-operation > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Tim Macatee Research Histology Core New York University School of Medicine 550 First Ave. Department of Pathology. Medical Science Building - Room 504 New York, N.Y. 10016 (212) 263-3888 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From HoustonR <@t> chi.osu.edu Fri Sep 14 10:44:42 2007 From: HoustonR <@t> chi.osu.edu (Houston, Ronald) Date: Fri Sep 14 10:45:27 2007 Subject: [Histonet] a funny for Friday In-Reply-To: Message-ID: <979FF5962E234F45B06CF0DB7C1AABB212026BEE@chi2k3ms01.columbuschildrens.net> A guy walks in to a bar and asks "Whose Chihuahua is that outside?" One guy answers "She's mine, why you asking?" "She's just killed my Doberman" came the reply. "What??????? How can a Chihuahua possibly kill a Doberman?" "Well, I think she got stuck in his throat" Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Columbus Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 houstonr@chi.osu.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Friday, September 14, 2007 12:25 PM To: 'Timothy Macatee'; 'Kemlo Rogerson'; 'Marshall Terry Dr,Consultant Histopathologist'; 'Roberta Horner'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] a funny for Friday A three-legged dog walks into a bar and says, "I'm lookin' for the man who shot my paw." Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Timothy Macatee Sent: Friday, September 14, 2007 9:55 AM To: Kemlo Rogerson; Marshall Terry Dr, Consultant Histopathologist; Roberta Horner; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] a funny for Friday A priest, a rabbi and the Dalai Lama walk into a bar. The bartender says, "Is this some kind of a joke?" On 9/14/07 9:46 AM, "Kemlo Rogerson" wrote: > A woman goes into a bar and asks for a "double entendre". So the > bartender gave her one. > > Kemlo Rogerson > Pathology Manager > DD 01934 647057 or extension 3311 > Mob 07749 754194; Pager 07659 597107; > > > The world breaks everyone, and afterward, some are strong at the broken > places. --Ernest Hemingway > > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. > Please inform me that this message has gone astray before deleting it. > Thank you for your co-operation > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Tim Macatee Research Histology Core New York University School of Medicine 550 First Ave. Department of Pathology. Medical Science Building - Room 504 New York, N.Y. 10016 (212) 263-3888 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From PMonfils <@t> Lifespan.org Fri Sep 14 10:46:50 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Sep 14 10:47:02 2007 Subject: [Histonet] a funny for Friday In-Reply-To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E273CDE@LSRIEXCH1.lsmaster.lifespan.org> A termite walks into a bar and asks "is the bar tender here?". From m5johnso <@t> meded.ucsd.edu Fri Sep 14 10:53:55 2007 From: m5johnso <@t> meded.ucsd.edu (Mindy Johnson) Date: Fri Sep 14 10:54:14 2007 Subject: [Histonet] a funny for Friday In-Reply-To: <979FF5962E234F45B06CF0DB7C1AABB212026BEE@chi2k3ms01.columbuschildrens.net> References: <979FF5962E234F45B06CF0DB7C1AABB212026BEE@chi2k3ms01.columbuschildrens.net> Message-ID: <013401c7f6e7$74b95610$5e2c0230$@ucsd.edu> Why was the mushroom so popular at the bar? Because he was a fun-guy!!! ?Mindy A Johnson SRA II UCSD - School of Medicine Medical Teaching Labs -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Friday, September 14, 2007 8:45 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] a funny for Friday A guy walks in to a bar and asks "Whose Chihuahua is that outside?" One guy answers "She's mine, why you asking?" "She's just killed my Doberman" came the reply. "What??????? How can a Chihuahua possibly kill a Doberman?" "Well, I think she got stuck in his throat" Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Columbus Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 houstonr@chi.osu.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Friday, September 14, 2007 12:25 PM To: 'Timothy Macatee'; 'Kemlo Rogerson'; 'Marshall Terry Dr,Consultant Histopathologist'; 'Roberta Horner'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] a funny for Friday A three-legged dog walks into a bar and says, "I'm lookin' for the man who shot my paw." Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Timothy Macatee Sent: Friday, September 14, 2007 9:55 AM To: Kemlo Rogerson; Marshall Terry Dr, Consultant Histopathologist; Roberta Horner; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] a funny for Friday A priest, a rabbi and the Dalai Lama walk into a bar. The bartender says, "Is this some kind of a joke?" On 9/14/07 9:46 AM, "Kemlo Rogerson" wrote: > A woman goes into a bar and asks for a "double entendre". So the > bartender gave her one. > > Kemlo Rogerson > Pathology Manager > DD 01934 647057 or extension 3311 > Mob 07749 754194; Pager 07659 597107; > > > The world breaks everyone, and afterward, some are strong at the broken > places. --Ernest Hemingway > > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. > Please inform me that this message has gone astray before deleting it. > Thank you for your co-operation > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Tim Macatee Research Histology Core New York University School of Medicine 550 First Ave. Department of Pathology. Medical Science Building - Room 504 New York, N.Y. 10016 (212) 263-3888 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. 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Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From simmonsca <@t> upmc.edu Fri Sep 14 10:56:26 2007 From: simmonsca <@t> upmc.edu (Simmons, Christopher) Date: Fri Sep 14 10:56:40 2007 Subject: [Histonet] a funny for Friday In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E273CDE@LSRIEXCH1.lsmaster.lifespan.org> References: <4EBFF65383B74D49995298C4976D1D5E273CDE@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <88D6F512ADC2CB428B90845D2D1388E303A5BB6E@1upmc-msx6.acct.upmchs.net> Beauty may be skin deep, but ugly goes to the bone. -Red Foxx Chris Simmons Lead Technologist UPMC Presbyterian Shadyside Histology 412.647.7660 desk 13242 pager -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Friday, September 14, 2007 11:47 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] a funny for Friday A termite walks into a bar and asks "is the bar tender here?". _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Fri Sep 14 11:03:05 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Sep 14 11:03:51 2007 Subject: [Histonet] a funny for Friday In-Reply-To: <979FF5962E234F45B06CF0DB7C1AABB212026BEE@chi2k3ms01.columbuschildrens.net> Message-ID: A duck walks into a bar - the bartender asks "What do you want?" Duck replies "Do you have any corn?". Bartender says "We don't have any corn" - duck leaves. The next day, the duck walks back into the bar again and asks "Do you have any corn"? Bartender says "Stupid duck, this is a BAR - we don't serve corn, and we don't like ducks. If you come back in here asking for corn again, I'll nail those flat feet of yours to the bar". Duck leaves. Next day, duck comes back into the same bar. The bartender crosses his arms over his chest and glares as the duck approaches the bar. Bartender growls, "What do you want". Duck says "Got any nails?" Bartender says "Nooooooooo....." Duck says, "Then I'd like some corn". From Reuel.Cornelia <@t> tsrh.org Fri Sep 14 11:11:21 2007 From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia) Date: Fri Sep 14 11:12:14 2007 Subject: [Histonet] a funny for Friday In-Reply-To: References: <979FF5962E234F45B06CF0DB7C1AABB212026BEE@chi2k3ms01.columbuschildrens.net> Message-ID: <46EA6C5A020000C50001C418@nwcl02.tsrh.org> man goes into a pet shop and tells the owner that he wants to buy a pet that can do everything. The owner says "How about a dog?" The man replies "Come on, a dog can't do everything." The owner says "How about a cat?" The man replies: "No way! A cat certainly can't do everything. I want a pet that can do everything! The owner thinks for a minute. Then says: "I've got it! ... A centipede!" The man says: "Centipede? ... I can't imagine a centipede doing everything but ... okay ... I'll try a centipede." He gets the centipede home and says to the centipede "Clean the kitchen." Thirty minutes later, he walks into the kitchen and ... it's immaculate. All the dishes and silverware have been washed, dried, and put away. The countertops cleaned. The appliances sparkling. The floor waxed. He's absolutely amazed. He says to the centipede "Go clean the living room." Twenty minutes later, he walks into the living room. The carpet has been vacuumed. The furniture cleaned and dusted. The pillows on the sofa plumped. Plants watered. The man thinks to himself "This is the most amazing thing I've ever seen. This is a pet that can really do everything." He says to the centipede: "Run down to the corner and get me a newspaper." The centipede walks out the door. 10 minutes later ... no centipede. 20 minutes later ... no centipede. 30 minutes later ... no centipede. The man is wondering what's going on. The centipede should have been back in a couple of minutes. 45 minutes later ... still no centipede The man can't imagine what could have happened. Did the centipede run away? Did it get run over by a car? Where is the centipede? He goes to the front door, opens it ... and there's the centipede sitting right outside the door. The man says "Hey!!! I sent you 45 minutes ago to run down to the corner and just get me a newspaper. What's the story?!" The centipede says "I'm goin'! I'm goin'! I'm puttin' on my shoes! ******************************************************************************************************************* Texas Scottish Rite Hospital for Children is one of the nation's leading pediatric centers for the treatment of orthopedic conditions, certain related neurological disorders and learning disorders, such as dyslexia. This email transmission and/or its attachments may contain confidential health information, intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing it to any other party unless required to do so by law and is required to delete/destroy the information after its stated need has been fulfilled. If you are not the intended recipient, any disclosure, copying, distribution or action taken in reliance on the contents of this email transmission is prohibited. If you have received this information in error, please notify the sender immediately and delete this information. We appreciate your efforts to protect the children's confidential information. ******************************************************************************************************************* From turkekul <@t> gmail.com Fri Sep 14 11:37:09 2007 From: turkekul <@t> gmail.com (mesruh turkekul) Date: Fri Sep 14 11:37:21 2007 Subject: [Histonet] K-CX for decalcification Message-ID: Dear all, Has anyone used K-CX decalcifiaction solution from Falma, Japan. What is your experience and how did you order it? Cheers, Mesru From alonso.martinezcanabal <@t> utoronto.ca Fri Sep 14 11:55:54 2007 From: alonso.martinezcanabal <@t> utoronto.ca (alonso.martinezcanabal@utoronto.ca) Date: Fri Sep 14 11:56:30 2007 Subject: [Histonet] Golgi-Cox issue Message-ID: <20070914125554.tp8mzo5us0owcc0k@webmail.utoronto.ca> Hi, I am working with Golgi-Cox sections, at 120 microns. I everything is very good, except that I cannot mount the sections very well, apparently the tickness is causing troubles and the sections get dry-like some days after. Any sugiestions to mount with permount the tick sections? Thank Yoy very much From rjbuesa <@t> yahoo.com Fri Sep 14 12:05:25 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Sep 14 12:05:40 2007 Subject: [Histonet] Golgi-Cox issue In-Reply-To: <20070914125554.tp8mzo5us0owcc0k@webmail.utoronto.ca> Message-ID: <544346.26169.qm@web61212.mail.yahoo.com> Use a coverslip at least twice the area of the section and do not dilute too much the Permount. Leave them flat and check in order to add more permount in the periphery if the Permount starts "to recede" from the borders. Ren? J. alonso.martinezcanabal@utoronto.ca wrote: Hi, I am working with Golgi-Cox sections, at 120 microns. I everything is very good, except that I cannot mount the sections very well, apparently the tickness is causing troubles and the sections get dry-like some days after. Any sugiestions to mount with permount the tick sections? Thank Yoy very much _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Fussy? Opinionated? Impossible to please? Perfect. Join Yahoo!'s user panel and lay it on us. From liz <@t> premierlab.com Fri Sep 14 12:07:19 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Sep 14 12:07:32 2007 Subject: [Histonet] Golgi-Cox issue In-Reply-To: <2129D99BFB394BFF844EDD0BE953F288@PremierLab.local> References: <2129D99BFB394BFF844EDD0BE953F288@PremierLab.local> Message-ID: Lots of mounting media and the smallest coverslip possible, that way you = have less chance for bubbles. When we mounted whole rat corneas we = placed the mounting media on top of the section and carefully placed the = coverslip over the top of the specimen. Then we used some nuts from the = hardware store and placed them on the coverglass to flatten out the = specimen and let dry overnight at room temp we found that heating the = slides caused more air bubbles. Liz=20 Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com =20 Ship to Address: =20 Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu = [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of = alonso.martinezcanabal@utoronto.ca Sent: Friday, September 14, 2007 11:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Golgi-Cox issue Hi, I am working with Golgi-Cox sections, at 120 microns. I = everything is very good, except that I cannot mount the sections very = well, apparently the tickness is causing troubles and the sections get = dry-like some days after. Any sugiestions to mount with permount the tick sections? Thank Yoy very much _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG Free Edition.=20 Version: 7.5.487 / Virus Database: 269.13.18/1007 - Release Date: = 9/13/2007 9:48 PM =20 No virus found in this outgoing message. Checked by AVG Free Edition.=20 Version: 7.5.487 / Virus Database: 269.13.18/1007 - Release Date: = 9/13/2007 9:48 PM =20 From jnocito <@t> satx.rr.com Fri Sep 14 12:10:52 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Sep 14 12:11:18 2007 Subject: [Histonet] RE: IHC and State requirements References: <002e01c7f621$75ab1b10$0202a8c0@yourxhtr8hvc4p> Message-ID: <00bf01c7f6f2$35359110$0202a8c0@yourxhtr8hvc4p> BUSTED, it was my toe ----- Original Message ----- From: "Edwards, R.E." To: "Joe Nocito" ; "Rene J Buesa" ; "Patricia Adams" ; "Bernice Frederick" ; "HistoNet" Sent: Friday, September 14, 2007 3:41 AM Subject: RE: [Histonet] RE: IHC and State requirements Experiences?,or was it your toe, Joe?. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: 13 September 2007 17:17 To: Rene J Buesa; Patricia Adams; Bernice Frederick; HistoNet Subject: Re: [Histonet] RE: IHC and State requirements to add to Rene's statement. My degrees, titles and experiences got my foot in the door, but it was my experiences that kept me there. JTT ----- Original Message ----- From: "Rene J Buesa" To: "Patricia Adams" ; "Bernice Frederick" ; "HistoNet" Sent: Thursday, September 13, 2007 10:56 AM Subject: RE: [Histonet] RE: IHC and State requirements > That is exactly my contention in an article I just finished about wages in > histology. > When you apply to a job, or negotiate your salary, you are not applying > as part of a group or class, you are negotiating your personal salary and > your experience and abilities are personal, particular, independent of > that of others, with or without a title. > From all the people I have supervised I have had the best amongst those > with lower academic levels, and, viceversa! > The title and the studies qualify you for a title, but does not > habilitate you for the tasks! > Ren? J. > > Patricia Adams wrote: > I am a HT, but have been doing IHC since it was > manual, doing up to 15 different antibodies at a > time and kept it all straight in my head. I am > not sure that having an HLT would have helped > with that. So where do some of us "older" techs > fit into the scheme of things. How do we let the > people hiring know that even though we do not > have the title, we know what we are doing? > Patricia > --- Bernice Frederick > wrote: > >> Hazel, >> I agree with you- I know people who are >> grandfathered and took the exam. >> I've met one person who helped create the HTL >> certification requirements. >> I'm just an HTL but do agree that some have the >> HTL but only know basic >> histo. I know of some institutions where an HTL >> is preferred when it comes >> to IHC (based on the BS). My pet peeve is >> post-docs that think they know >> everything but can't comprehend histo and >> thinek they can do microtomy. We >> require certification for those people that are >> cutting blocks that are >> human cancer trial specimens (a long story) >> Bernice >> >> Bernice Frederick HTL (ASCP) >> Northwestern University >> Pathology Core Facility >> 710 N Fairbanks Court >> Olson 8-421 >> Chicago,IL 60611 >> 312-503-3723 >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> > [mailto:histonet-bounces@lists.utsouthwestern.edu] >> On Behalf Of Horn, Hazel >> V >> Sent: Thursday, September 13, 2007 9:54 AM >> To: Kim Tournear; >> histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] RE: IHC and State >> requirements >> >> Being grandfathered in did not mean they just >> gave you the title of HTL. >> The grandfathered HTL's had to pass the tests >> just like anyone else! >> >> Hazel Horn >> Hazel Horn, HT/HTL (ASCP) >> Supervisor of Histology >> Arkansas Children's Hospital >> 800 Marshall Slot 820 >> Little Rock, AR 72202 >> >> phone 501.364.4240 >> fax 501.364.3912 >> >> visit us on the web at: www.archildrens.org >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> > [mailto:histonet-bounces@lists.utsouthwestern.edu] >> On Behalf Of Kim >> Tournear >> Sent: Thursday, September 13, 2007 9:27 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] RE: IHC and State >> requirements >> >> Hi Michelle, >> A lot employers will except either HT or HTL. >> And of course, how much >> experience you have under your belt is also a >> big consideration. I >> think a lot of employers are finding out just >> how hard it is to find >> techs (registered or not) that can do the job >> and do it well, although >> there are a few states with their own state >> requirements which would >> require the tech to be ASCP certified either >> way. >> >> I have found that being an HTL vs HT doesn't >> necessarily mean that one >> or the other can do the job better...I know a >> few HTL's that were >> grandfathered in and have done only a handful >> IHCs in their whole >> career, can't trouble shoot anything, and >> produce poor quality slides >> and yet they hold the title of an HTL and the >> same goes for the >> HTs...(makes you wonder how they keep a >> job)...LOL....and I have taught >> and worked with students right out of college >> programs that were >> absolutely awesome and knew their stuff....go >> figure.....I say go for >> it.....Good Luck.... >> >> >> Kim Tournear, HT (ASCP), QIHC ( ASCP) >> Specialists in Dermatology >> Histology/Mohs Supervisor >> Tucson, AZ >> >> >> >> >> --------------------------------- >> Boardwalk for $500? In 2007? Ha! >> Play Monopoly Here and Now (it's updated for >> today's economy) at Yahoo! >> Games. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > ---------------------------------------------------------------------------- >> -- >> The information contained in this message may >> be privileged and confidential >> and protected from disclosure. If the reader of >> this message is not the >> intended recipient, or an employee or agent >> responsible for delivering this >> message to the intended recipient, you are >> hereby notified that any >> dissemination, distribution or copying of this >> communication is strictly >> prohibited. If you have received this >> communication in error, please notify >> us immediately by replying to the message and >> deleting it from your >> computer. >> Thank you. >> > ============================================================================ >> == >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > Patricia Adams > ----- > Fight back spam! Download the Blue Frog. > http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D > > > > ____________________________________________________________________________________ > Yahoo! oneSearch: Finally, mobile search > that gives answers, not web links. > http://mobile.yahoo.com/mobileweb/onesearch?refer=1ONXIC > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Be a better Heartthrob. Get better relationship answers from someone who > knows. > Yahoo! Answers - Check it out. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Fri Sep 14 12:24:16 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Sep 14 12:24:36 2007 Subject: [Histonet] a funny for Friday References: <68ggkh$13mb68i@hrndva-mx-15.mgw.rr.com> Message-ID: <014d01c7f6f4$190a7f80$0202a8c0@yourxhtr8hvc4p> a blonde who lives in Nevada started her own handyman business. She goes out all day trying to get a job but know one hires her because she is a blonde. Now it's mid-afternoon with the over 100 degrees. She knocks on this one door and a guy answers. She tells the guy, "Please sir, I've started my own business and have been pounding the pavement all day. Is there any job I can do for you?" can paint my porch." the man said. "I'll bring the paint and brushes out." A couple hours later, the man comes out of his house and sees the blonde wearing two overcoats. "Why are you wearing coats? It's over 100 degrees" he said. The blonde responds "See, I'm not a stupid blonde. The directions says "For better results, use 2 coats". ----- Original Message ----- From: "Douglas D Deltour" To: "'Timothy Macatee'" ; "'Kemlo Rogerson'" ; "'Marshall Terry Dr, Consultant Histopathologist'" ; "'Roberta Horner'" ; Sent: Friday, September 14, 2007 11:24 AM Subject: RE: [Histonet] a funny for Friday >A three-legged dog walks into a bar and says, "I'm lookin' for the man who > shot my paw." > > Douglas D. Deltour HT(ASCP) > Histology Manager > Professional Pathology Services, PC > One Science Court > Suite 200 > Columbia, SC 29203 > Office (803)252-1913 > Fax (803)254-3262 > Doug@ppspath.com > ***************************************************** > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of the individual or entity to > which it is addressed and may contain information that is privileged, > confidential and exempt from disclosure under applicable law. If the > reader > of this message is not the intended recipient, you are hereby notified > that > any dissemination, distribution, or copying of this communication is > strictly prohibited by law. If you have received this communication in > error, please notify me immediately. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Timothy > Macatee > Sent: Friday, September 14, 2007 9:55 AM > To: Kemlo Rogerson; Marshall Terry Dr, Consultant Histopathologist; > Roberta > Horner; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] a funny for Friday > > A priest, a rabbi and the Dalai Lama walk into a bar. The bartender says, > "Is this some kind of a joke?" > > > > On 9/14/07 9:46 AM, "Kemlo Rogerson" > wrote: > >> A woman goes into a bar and asks for a "double entendre". So the >> bartender gave her one. >> >> Kemlo Rogerson >> Pathology Manager >> DD 01934 647057 or extension 3311 >> Mob 07749 754194; Pager 07659 597107; >> >> >> The world breaks everyone, and afterward, some are strong at the broken >> places. --Ernest Hemingway >> >> This e-mail is confidential and privileged. If you are not the intended >> recipient please accept my apologies; please do not disclose, copy or >> distribute information in this e-mail or take any action in reliance on >> its contents: to do so is strictly prohibited and may be unlawful. >> Please inform me that this message has gone astray before deleting it. >> Thank you for your co-operation >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > -- > Tim Macatee > Research Histology Core > New York University School of Medicine > 550 First Ave. > Department of Pathology. > Medical Science Building - Room 504 > New York, N.Y. 10016 > (212) 263-3888 > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Fri Sep 14 12:10:52 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Sep 14 12:24:42 2007 Subject: [Histonet] RE: IHC and State requirements References: <002e01c7f621$75ab1b10$0202a8c0@yourxhtr8hvc4p> Message-ID: <014c01c7f6f4$16dc7bf0$0202a8c0@yourxhtr8hvc4p> BUSTED, it was my toe ----- Original Message ----- From: "Edwards, R.E." To: "Joe Nocito" ; "Rene J Buesa" ; "Patricia Adams" ; "Bernice Frederick" ; "HistoNet" Sent: Friday, September 14, 2007 3:41 AM Subject: RE: [Histonet] RE: IHC and State requirements Experiences?,or was it your toe, Joe?. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: 13 September 2007 17:17 To: Rene J Buesa; Patricia Adams; Bernice Frederick; HistoNet Subject: Re: [Histonet] RE: IHC and State requirements to add to Rene's statement. My degrees, titles and experiences got my foot in the door, but it was my experiences that kept me there. JTT ----- Original Message ----- From: "Rene J Buesa" To: "Patricia Adams" ; "Bernice Frederick" ; "HistoNet" Sent: Thursday, September 13, 2007 10:56 AM Subject: RE: [Histonet] RE: IHC and State requirements > That is exactly my contention in an article I just finished about wages in > histology. > When you apply to a job, or negotiate your salary, you are not applying > as part of a group or class, you are negotiating your personal salary and > your experience and abilities are personal, particular, independent of > that of others, with or without a title. > From all the people I have supervised I have had the best amongst those > with lower academic levels, and, viceversa! > The title and the studies qualify you for a title, but does not > habilitate you for the tasks! > Ren? J. > > Patricia Adams wrote: > I am a HT, but have been doing IHC since it was > manual, doing up to 15 different antibodies at a > time and kept it all straight in my head. I am > not sure that having an HLT would have helped > with that. So where do some of us "older" techs > fit into the scheme of things. How do we let the > people hiring know that even though we do not > have the title, we know what we are doing? > Patricia > --- Bernice Frederick > wrote: > >> Hazel, >> I agree with you- I know people who are >> grandfathered and took the exam. >> I've met one person who helped create the HTL >> certification requirements. >> I'm just an HTL but do agree that some have the >> HTL but only know basic >> histo. I know of some institutions where an HTL >> is preferred when it comes >> to IHC (based on the BS). My pet peeve is >> post-docs that think they know >> everything but can't comprehend histo and >> thinek they can do microtomy. We >> require certification for those people that are >> cutting blocks that are >> human cancer trial specimens (a long story) >> Bernice >> >> Bernice Frederick HTL (ASCP) >> Northwestern University >> Pathology Core Facility >> 710 N Fairbanks Court >> Olson 8-421 >> Chicago,IL 60611 >> 312-503-3723 >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> > [mailto:histonet-bounces@lists.utsouthwestern.edu] >> On Behalf Of Horn, Hazel >> V >> Sent: Thursday, September 13, 2007 9:54 AM >> To: Kim Tournear; >> histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] RE: IHC and State >> requirements >> >> Being grandfathered in did not mean they just >> gave you the title of HTL. >> The grandfathered HTL's had to pass the tests >> just like anyone else! >> >> Hazel Horn >> Hazel Horn, HT/HTL (ASCP) >> Supervisor of Histology >> Arkansas Children's Hospital >> 800 Marshall Slot 820 >> Little Rock, AR 72202 >> >> phone 501.364.4240 >> fax 501.364.3912 >> >> visit us on the web at: www.archildrens.org >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> > [mailto:histonet-bounces@lists.utsouthwestern.edu] >> On Behalf Of Kim >> Tournear >> Sent: Thursday, September 13, 2007 9:27 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] RE: IHC and State >> requirements >> >> Hi Michelle, >> A lot employers will except either HT or HTL. >> And of course, how much >> experience you have under your belt is also a >> big consideration. I >> think a lot of employers are finding out just >> how hard it is to find >> techs (registered or not) that can do the job >> and do it well, although >> there are a few states with their own state >> requirements which would >> require the tech to be ASCP certified either >> way. >> >> I have found that being an HTL vs HT doesn't >> necessarily mean that one >> or the other can do the job better...I know a >> few HTL's that were >> grandfathered in and have done only a handful >> IHCs in their whole >> career, can't trouble shoot anything, and >> produce poor quality slides >> and yet they hold the title of an HTL and the >> same goes for the >> HTs...(makes you wonder how they keep a >> job)...LOL....and I have taught >> and worked with students right out of college >> programs that were >> absolutely awesome and knew their stuff....go >> figure.....I say go for >> it.....Good Luck.... >> >> >> Kim Tournear, HT (ASCP), QIHC ( ASCP) >> Specialists in Dermatology >> Histology/Mohs Supervisor >> Tucson, AZ >> >> >> >> >> --------------------------------- >> Boardwalk for $500? In 2007? Ha! >> Play Monopoly Here and Now (it's updated for >> today's economy) at Yahoo! >> Games. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > ---------------------------------------------------------------------------- >> -- >> The information contained in this message may >> be privileged and confidential >> and protected from disclosure. If the reader of >> this message is not the >> intended recipient, or an employee or agent >> responsible for delivering this >> message to the intended recipient, you are >> hereby notified that any >> dissemination, distribution or copying of this >> communication is strictly >> prohibited. If you have received this >> communication in error, please notify >> us immediately by replying to the message and >> deleting it from your >> computer. >> Thank you. >> > ============================================================================ >> == >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > Patricia Adams > ----- > Fight back spam! Download the Blue Frog. > http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D > > > > ____________________________________________________________________________________ > Yahoo! oneSearch: Finally, mobile search > that gives answers, not web links. > http://mobile.yahoo.com/mobileweb/onesearch?refer=1ONXIC > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Be a better Heartthrob. Get better relationship answers from someone who > knows. > Yahoo! Answers - Check it out. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From esther.peters <@t> verizon.net Fri Sep 14 12:43:40 2007 From: esther.peters <@t> verizon.net (Esther Peters) Date: Fri Sep 14 12:43:20 2007 Subject: [Histonet] AO 860 sliding microtome and Caroline Bass In-Reply-To: <6.0.0.22.1.20070913151904.01b126f8@gemini.msu.montana.edu> References: <6.0.0.22.1.20070913151904.01b126f8@gemini.msu.montana.edu> Message-ID: <46EAC84C.7080505@verizon.net> I have two of those long blades that I would be interested in selling to or trading for other supplies with someone! Esther Peters George Mason University Gayle Callis wrote: > Caroline, > > If you send me your slowmail, US postal service address, I will send > you a manual on the effective use and proper care of the microtome. > This little green booklet (collectors item!) tells how to operate the > 860 along with other AO microtomes (820 and freezing microtome). I > found an extra copy in my file this past month. > > As for disposable blades in this sliding microtome, we always used a c > profile steel knife, a gigantic blade 200 mm long, for study, > vibration free operation. The clamp may not work with a disposable > holder. Dorn and Hart is a good place to get > resharpening/reconditioning of the blades or do that with a > ThermoShandon whatever name now knife sharpener. This was a superb > sharpener and the microtome is a oldie but a goodie, heavy duty finger > amputator. We also used Bear Oil, a microtome oil which is still > available although Rene's suggestion for the Leica oil is ideal too. > > Be careful, these microtomes are very efficient for sectioning and > cutting YOU!!! It was the first sliding microtome I used for > sectioning celloidin embedded monkey temporal mandibular joints back > in 1963. It was fun, but I did work with closed doors during its > operation to avoid the startle factor during sectioning. I kept my > fingers, arms, etc intact. > > Good luck with your "new" toy. > > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From msatterfield <@t> parkwaysc.org Fri Sep 14 12:56:44 2007 From: msatterfield <@t> parkwaysc.org (Mari Satterfield) Date: Fri Sep 14 12:58:44 2007 Subject: [Histonet] Eosinophilic PNB Message-ID: I wondered if anyone could give me any suggestions as to what might be the cause of random eosinophilic artifact in prostate needle biopsies. Sometimes not even the whole core is effected. Any suggestions would be greatly appreciated. MS From mmccoy100 <@t> gmail.com Fri Sep 14 13:11:25 2007 From: mmccoy100 <@t> gmail.com (Michelle McCoy) Date: Fri Sep 14 13:11:38 2007 Subject: [Histonet] States that require license In-Reply-To: References: <25355ef80709132201r5b1b0950ya08043f41444700b@mail.gmail.com> Message-ID: <25355ef80709141111k41a7a56foab9a34bf70fe36b4@mail.gmail.com> Hi, Great info! Did the grandfathered techs have to do any testing then? I believe a previous post mentioned alot of grandfathered techs had to be tested when state licensing went into effect in some states. I believe the FL history is that grandfathered techs took the FL test (not ASCP), but that FL no longer offers this test and the only testing now is ASCP. So I guess so for only FL, NY are states requiring licensing-any others??? On 9/14/07, Luis Chiriboga wrote: > > Hi everyone > I'm getting into this thread a bit late as I was not receiving Histonet > messages for the last couple of weeks. A colleague mentioned this thread > and > I thought I should follow-up. I apologize if this information has already > been posted. > > Michelle, I'm not sure what your original post was but I understand there > was some question regarding licensing requirements and certification in > different states. I can only speak for New York State.... > > You are correct, NYS now requires any individual working in a clinical > laboratory to be licensed. This law went into effect last September 06 > with > a grand fathering provision that just expired this September. I think its > important to recognize that there is a significant difference between > "licensing" and "certification". "Licensing" is performed by a government > organization while "certification" can be performed by almost any > organization. Obviously, the value of a certification is based on the > veracity of the organization providing it. While it is also true that > many > other states view ASCP as a valid certifying agency and, ASCP > certification > as a measure of competency, the NYS education department (NYSED) has > chosen > not to accept ASCP certification as a means of credentialing for grand > fathering or as a means to establish competency in the absence or presence > of an examination for any laboratory discipline. However, I believe that > NYSED has selected ASCP to create and administer a "generalist" exam based > on the educational curriculum mandated by the NYSED and the Clinical > Laboratory Technology Practice Act. > > As the law currently stands in NYS, anyone who was not grad fathered will > have to meet the educational requirements AND take the licensing > examination > in order to be employed in NYS (for detail info on the curriculum > material, > please see article 165 of the education law > http://www.op.nysed.gov/clp.htm). > > In NYS, this law applies to all clinical laboratory personnel, including > histologist. NYS does not recognize nor require ASCP certification for > licensing. > > For those of you in other states that are considering proposing a > licensing > law (I think I saw a message thread from a few on this), I suggest that > you > take a look at the NYS law...... > I hope this helps. If you have any questions, please feel free to respond. > Luis > > Luis Chiriboga PhD., HT (ASCP) QIHC > New York University School of Medicine > NYU Cancer Institute IHC Core Facility > Bellevue Hospital Center > Department of Pathology 4W27 > 27th Street & First Avenue > New York, N.Y. 10016 > V (212)562-4667 > F (212)263-2041 > > > Vice President > New York State Histotechnological Society > http://www.nyhisto.org/ > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Michelle > McCoy > Sent: Friday, September 14, 2007 12:01 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] States that require license > > > Someone in a recent previous post had stated that they thought only one > state required state licensing-I know NY and FL do now. I read in an > article > about NY state licensing http://www.medscape.com/viewarticle/542005 > law this excerpt: > > *With one exception (WV), all states requiring licensure (CA, FL, GA, HI, > LA, MT, NV, ND, PR, RI, TN) recognize the American Society for Clinical > Pathology (ASCP) as an approved certifying agency.* > *Commissioner's regulations require candidates to pass a generalist > examination for CLT and CT. With the exception of the grandparenting > period > (through September 1, 2008) specialists without generalist certification > will be required to take a generalist examination to qualify for > licensure.* > ** > -I am hoping to find out if this law applies to Histology as well > -If this is an up to date list of the states that require it, if so > -If grandfathered techs in these states had to take ascp or if they took a > different state test to get the state license. > -Is there any site anyone knows about that keeps up to date information on > what all the different states are doing regarding licensure requirements > in > our field other than sifting through the archives here at histonet? > Thanks for any input- > Michelle > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From b-frederick <@t> northwestern.edu Fri Sep 14 13:37:30 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Sep 14 13:37:39 2007 Subject: [Histonet] States that require license In-Reply-To: <25355ef80709141111k41a7a56foab9a34bf70fe36b4@mail.gmail.com> Message-ID: <000b01c7f6fe$51cd17b0$d00f7ca5@lurie.northwestern.edu> I know of couple techs that did have to test. The grandfathering meant theat those wanting the HTL did not have to have a bachelor's but years of experience as well as the HT certification. Onr missed it by a percentage point and stayed an HT (1977). My number is low and I took the HTL in 1985. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michelle McCoy Sent: Friday, September 14, 2007 1:11 PM To: Luis Chiriboga Cc: histonet@lists.utsouthwestern.edu; Amy Farnan; Mary Georger Subject: Re: [Histonet] States that require license Hi, Great info! Did the grandfathered techs have to do any testing then? I believe a previous post mentioned alot of grandfathered techs had to be tested when state licensing went into effect in some states. I believe the FL history is that grandfathered techs took the FL test (not ASCP), but that FL no longer offers this test and the only testing now is ASCP. So I guess so for only FL, NY are states requiring licensing-any others??? On 9/14/07, Luis Chiriboga wrote: > > Hi everyone > I'm getting into this thread a bit late as I was not receiving Histonet > messages for the last couple of weeks. A colleague mentioned this thread > and > I thought I should follow-up. I apologize if this information has already > been posted. > > Michelle, I'm not sure what your original post was but I understand there > was some question regarding licensing requirements and certification in > different states. I can only speak for New York State.... > > You are correct, NYS now requires any individual working in a clinical > laboratory to be licensed. This law went into effect last September 06 > with > a grand fathering provision that just expired this September. I think its > important to recognize that there is a significant difference between > "licensing" and "certification". "Licensing" is performed by a government > organization while "certification" can be performed by almost any > organization. Obviously, the value of a certification is based on the > veracity of the organization providing it. While it is also true that > many > other states view ASCP as a valid certifying agency and, ASCP > certification > as a measure of competency, the NYS education department (NYSED) has > chosen > not to accept ASCP certification as a means of credentialing for grand > fathering or as a means to establish competency in the absence or presence > of an examination for any laboratory discipline. However, I believe that > NYSED has selected ASCP to create and administer a "generalist" exam based > on the educational curriculum mandated by the NYSED and the Clinical > Laboratory Technology Practice Act. > > As the law currently stands in NYS, anyone who was not grad fathered will > have to meet the educational requirements AND take the licensing > examination > in order to be employed in NYS (for detail info on the curriculum > material, > please see article 165 of the education law > http://www.op.nysed.gov/clp.htm). > > In NYS, this law applies to all clinical laboratory personnel, including > histologist. NYS does not recognize nor require ASCP certification for > licensing. > > For those of you in other states that are considering proposing a > licensing > law (I think I saw a message thread from a few on this), I suggest that > you > take a look at the NYS law...... > I hope this helps. If you have any questions, please feel free to respond. > Luis > > Luis Chiriboga PhD., HT (ASCP) QIHC > New York University School of Medicine > NYU Cancer Institute IHC Core Facility > Bellevue Hospital Center > Department of Pathology 4W27 > 27th Street & First Avenue > New York, N.Y. 10016 > V (212)562-4667 > F (212)263-2041 > > > Vice President > New York State Histotechnological Society > http://www.nyhisto.org/ > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Michelle > McCoy > Sent: Friday, September 14, 2007 12:01 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] States that require license > > > Someone in a recent previous post had stated that they thought only one > state required state licensing-I know NY and FL do now. I read in an > article > about NY state licensing http://www.medscape.com/viewarticle/542005 > law this excerpt: > > *With one exception (WV), all states requiring licensure (CA, FL, GA, HI, > LA, MT, NV, ND, PR, RI, TN) recognize the American Society for Clinical > Pathology (ASCP) as an approved certifying agency.* > *Commissioner's regulations require candidates to pass a generalist > examination for CLT and CT. With the exception of the grandparenting > period > (through September 1, 2008) specialists without generalist certification > will be required to take a generalist examination to qualify for > licensure.* > ** > -I am hoping to find out if this law applies to Histology as well > -If this is an up to date list of the states that require it, if so > -If grandfathered techs in these states had to take ascp or if they took a > different state test to get the state license. > -Is there any site anyone knows about that keeps up to date information on > what all the different states are doing regarding licensure requirements > in > our field other than sifting through the archives here at histonet? > Thanks for any input- > Michelle > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Luis.Chiriboga <@t> med.nyu.edu Fri Sep 14 14:51:54 2007 From: Luis.Chiriboga <@t> med.nyu.edu (Luis Chiriboga) Date: Fri Sep 14 13:46:08 2007 Subject: [Histonet] States that require license In-Reply-To: <25355ef80709141111k41a7a56foab9a34bf70fe36b4@mail.gmail.com> Message-ID: Grandfathered individuals did not and will not be required to take the examination. Because of the wide range of education and training (primarily in NYC), from no formal education to extensive education and training, there were several routes available with specific criteria to apply for grandfathering. I do not believe that an exam has yet been created. Luis -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Michelle McCoy Sent: Friday, September 14, 2007 1:11 PM To: Luis Chiriboga Cc: histonet@lists.utsouthwestern.edu; Amy Farnan; Mary Georger Subject: Re: [Histonet] States that require license Hi, Great info! Did the grandfathered techs have to do any testing then? I believe a previous post mentioned alot of grandfathered techs had to be tested when state licensing went into effect in some states. I believe the FL history is that grandfathered techs took the FL test (not ASCP), but that FL no longer offers this test and the only testing now is ASCP. So I guess so for only FL, NY are states requiring licensing-any others??? On 9/14/07, Luis Chiriboga wrote: > > Hi everyone > I'm getting into this thread a bit late as I was not receiving Histonet > messages for the last couple of weeks. A colleague mentioned this thread > and > I thought I should follow-up. I apologize if this information has already > been posted. > > Michelle, I'm not sure what your original post was but I understand there > was some question regarding licensing requirements and certification in > different states. I can only speak for New York State.... > > You are correct, NYS now requires any individual working in a clinical > laboratory to be licensed. This law went into effect last September 06 > with > a grand fathering provision that just expired this September. I think its > important to recognize that there is a significant difference between > "licensing" and "certification". "Licensing" is performed by a government > organization while "certification" can be performed by almost any > organization. Obviously, the value of a certification is based on the > veracity of the organization providing it. While it is also true that > many > other states view ASCP as a valid certifying agency and, ASCP > certification > as a measure of competency, the NYS education department (NYSED) has > chosen > not to accept ASCP certification as a means of credentialing for grand > fathering or as a means to establish competency in the absence or presence > of an examination for any laboratory discipline. However, I believe that > NYSED has selected ASCP to create and administer a "generalist" exam based > on the educational curriculum mandated by the NYSED and the Clinical > Laboratory Technology Practice Act. > > As the law currently stands in NYS, anyone who was not grad fathered will > have to meet the educational requirements AND take the licensing > examination > in order to be employed in NYS (for detail info on the curriculum > material, > please see article 165 of the education law > http://www.op.nysed.gov/clp.htm). > > In NYS, this law applies to all clinical laboratory personnel, including > histologist. NYS does not recognize nor require ASCP certification for > licensing. > > For those of you in other states that are considering proposing a > licensing > law (I think I saw a message thread from a few on this), I suggest that > you > take a look at the NYS law...... > I hope this helps. If you have any questions, please feel free to respond. > Luis > > Luis Chiriboga PhD., HT (ASCP) QIHC > New York University School of Medicine > NYU Cancer Institute IHC Core Facility > Bellevue Hospital Center > Department of Pathology 4W27 > 27th Street & First Avenue > New York, N.Y. 10016 > V (212)562-4667 > F (212)263-2041 > > > Vice President > New York State Histotechnological Society > http://www.nyhisto.org/ > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Michelle > McCoy > Sent: Friday, September 14, 2007 12:01 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] States that require license > > > Someone in a recent previous post had stated that they thought only one > state required state licensing-I know NY and FL do now. I read in an > article > about NY state licensing http://www.medscape.com/viewarticle/542005 > law this excerpt: > > *With one exception (WV), all states requiring licensure (CA, FL, GA, HI, > LA, MT, NV, ND, PR, RI, TN) recognize the American Society for Clinical > Pathology (ASCP) as an approved certifying agency.* > *Commissioner's regulations require candidates to pass a generalist > examination for CLT and CT. With the exception of the grandparenting > period > (through September 1, 2008) specialists without generalist certification > will be required to take a generalist examination to qualify for > licensure.* > ** > -I am hoping to find out if this law applies to Histology as well > -If this is an up to date list of the states that require it, if so > -If grandfathered techs in these states had to take ascp or if they took a > different state test to get the state license. > -Is there any site anyone knows about that keeps up to date information on > what all the different states are doing regarding licensure requirements > in > our field other than sifting through the archives here at histonet? > Thanks for any input- > Michelle > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Pat.Bell <@t> UCHSC.edu Fri Sep 14 13:56:06 2007 From: Pat.Bell <@t> UCHSC.edu (Pat.Bell@UCHSC.edu) Date: Fri Sep 14 13:56:24 2007 Subject: [Histonet] States that require license In-Reply-To: Message-ID: <71FCC52823941D49A4BC39B48C6E3428F3BDE6@java.uchsc.edu> Grandfathered individuals had to take a written test and also had to do a practical. Pat Bell; HT(ASCP) University of Co. Health Sciences Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Luis Chiriboga Sent: Friday, September 14, 2007 1:52 PM To: Michelle McCoy Cc: histonet@lists.utsouthwestern.edu; Amy Farnan; Mary Georger Subject: RE: [Histonet] States that require license Grandfathered individuals did not and will not be required to take the examination. Because of the wide range of education and training (primarily in NYC), from no formal education to extensive education and training, there were several routes available with specific criteria to apply for grandfathering. I do not believe that an exam has yet been created. Luis -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Michelle McCoy Sent: Friday, September 14, 2007 1:11 PM To: Luis Chiriboga Cc: histonet@lists.utsouthwestern.edu; Amy Farnan; Mary Georger Subject: Re: [Histonet] States that require license Hi, Great info! Did the grandfathered techs have to do any testing then? I believe a previous post mentioned alot of grandfathered techs had to be tested when state licensing went into effect in some states. I believe the FL history is that grandfathered techs took the FL test (not ASCP), but that FL no longer offers this test and the only testing now is ASCP. So I guess so for only FL, NY are states requiring licensing-any others??? On 9/14/07, Luis Chiriboga wrote: > > Hi everyone > I'm getting into this thread a bit late as I was not receiving Histonet > messages for the last couple of weeks. A colleague mentioned this thread > and > I thought I should follow-up. I apologize if this information has already > been posted. > > Michelle, I'm not sure what your original post was but I understand there > was some question regarding licensing requirements and certification in > different states. I can only speak for New York State.... > > You are correct, NYS now requires any individual working in a clinical > laboratory to be licensed. This law went into effect last September 06 > with > a grand fathering provision that just expired this September. I think its > important to recognize that there is a significant difference between > "licensing" and "certification". "Licensing" is performed by a government > organization while "certification" can be performed by almost any > organization. Obviously, the value of a certification is based on the > veracity of the organization providing it. While it is also true that > many > other states view ASCP as a valid certifying agency and, ASCP > certification > as a measure of competency, the NYS education department (NYSED) has > chosen > not to accept ASCP certification as a means of credentialing for grand > fathering or as a means to establish competency in the absence or presence > of an examination for any laboratory discipline. However, I believe that > NYSED has selected ASCP to create and administer a "generalist" exam based > on the educational curriculum mandated by the NYSED and the Clinical > Laboratory Technology Practice Act. > > As the law currently stands in NYS, anyone who was not grad fathered will > have to meet the educational requirements AND take the licensing > examination > in order to be employed in NYS (for detail info on the curriculum > material, > please see article 165 of the education law > http://www.op.nysed.gov/clp.htm). > > In NYS, this law applies to all clinical laboratory personnel, including > histologist. NYS does not recognize nor require ASCP certification for > licensing. > > For those of you in other states that are considering proposing a > licensing > law (I think I saw a message thread from a few on this), I suggest that > you > take a look at the NYS law...... > I hope this helps. If you have any questions, please feel free to respond. > Luis > > Luis Chiriboga PhD., HT (ASCP) QIHC > New York University School of Medicine > NYU Cancer Institute IHC Core Facility > Bellevue Hospital Center > Department of Pathology 4W27 > 27th Street & First Avenue > New York, N.Y. 10016 > V (212)562-4667 > F (212)263-2041 > > > Vice President > New York State Histotechnological Society > http://www.nyhisto.org/ > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Michelle > McCoy > Sent: Friday, September 14, 2007 12:01 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] States that require license > > > Someone in a recent previous post had stated that they thought only one > state required state licensing-I know NY and FL do now. I read in an > article > about NY state licensing http://www.medscape.com/viewarticle/542005 > law this excerpt: > > *With one exception (WV), all states requiring licensure (CA, FL, GA, HI, > LA, MT, NV, ND, PR, RI, TN) recognize the American Society for Clinical > Pathology (ASCP) as an approved certifying agency.* > *Commissioner's regulations require candidates to pass a generalist > examination for CLT and CT. With the exception of the grandparenting > period > (through September 1, 2008) specialists without generalist certification > will be required to take a generalist examination to qualify for > licensure.* > ** > -I am hoping to find out if this law applies to Histology as well > -If this is an up to date list of the states that require it, if so > -If grandfathered techs in these states had to take ascp or if they took a > different state test to get the state license. > -Is there any site anyone knows about that keeps up to date information on > what all the different states are doing regarding licensure requirements > in > our field other than sifting through the archives here at histonet? > Thanks for any input- > Michelle > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Fri Sep 14 14:07:16 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 14 14:06:43 2007 Subject: [Histonet] I think I will give it an acronym Message-ID: <6.0.0.22.1.20070914130106.01aff958@gemini.msu.montana.edu> Curtis, I think I will now assign an acronym, TFSci or maybe ThermFishSci. Too long to type out all the time. >Subject: RE: [Histonet] AO 860 sliding microtome and Caroline Bass >Date: Fri, 14 Sep 2007 14:03:12 -0400 > >From: "King, Curtis - RAS" > >Just to clear things up The Company Name is ThermoFisher Scientific. > >Thermo Shandon is a Brand just like Thermo Richard Allen is now a brand. > >Curtis D King, HT (ASCP) >Research and Development >ThermoFisher Scientific >4481 Campus Drive >Kalamazoo, MI 49008 >800-522-7270 ext. 672 >Curtis.King@thermofisher.com > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From Luis.Chiriboga <@t> med.nyu.edu Fri Sep 14 15:22:42 2007 From: Luis.Chiriboga <@t> med.nyu.edu (Luis Chiriboga) Date: Fri Sep 14 14:17:09 2007 Subject: [Histonet] States that require license In-Reply-To: <71FCC52823941D49A4BC39B48C6E3428F3BDE6@java.uchsc.edu> Message-ID: just for clarification, I am specifically referring to New York State Licensing only, not to any other certification (ASCP) procedure Luis -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Pat.Bell@UCHSC.edu Sent: Friday, September 14, 2007 1:56 PM To: Luis.Chiriboga@med.nyu.edu; mmccoy100@gmail.com Cc: histonet@lists.utsouthwestern.edu; Farnana@nehealth.com; Mary_Georger@urmc.rochester.edu Subject: RE: [Histonet] States that require license Grandfathered individuals had to take a written test and also had to do a practical. Pat Bell; HT(ASCP) University of Co. Health Sciences Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Luis Chiriboga Sent: Friday, September 14, 2007 1:52 PM To: Michelle McCoy Cc: histonet@lists.utsouthwestern.edu; Amy Farnan; Mary Georger Subject: RE: [Histonet] States that require license Grandfathered individuals did not and will not be required to take the examination. Because of the wide range of education and training (primarily in NYC), from no formal education to extensive education and training, there were several routes available with specific criteria to apply for grandfathering. I do not believe that an exam has yet been created. Luis -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Michelle McCoy Sent: Friday, September 14, 2007 1:11 PM To: Luis Chiriboga Cc: histonet@lists.utsouthwestern.edu; Amy Farnan; Mary Georger Subject: Re: [Histonet] States that require license Hi, Great info! Did the grandfathered techs have to do any testing then? I believe a previous post mentioned alot of grandfathered techs had to be tested when state licensing went into effect in some states. I believe the FL history is that grandfathered techs took the FL test (not ASCP), but that FL no longer offers this test and the only testing now is ASCP. So I guess so for only FL, NY are states requiring licensing-any others??? On 9/14/07, Luis Chiriboga wrote: > > Hi everyone > I'm getting into this thread a bit late as I was not receiving Histonet > messages for the last couple of weeks. A colleague mentioned this thread > and > I thought I should follow-up. I apologize if this information has already > been posted. > > Michelle, I'm not sure what your original post was but I understand there > was some question regarding licensing requirements and certification in > different states. I can only speak for New York State.... > > You are correct, NYS now requires any individual working in a clinical > laboratory to be licensed. This law went into effect last September 06 > with > a grand fathering provision that just expired this September. I think its > important to recognize that there is a significant difference between > "licensing" and "certification". "Licensing" is performed by a government > organization while "certification" can be performed by almost any > organization. Obviously, the value of a certification is based on the > veracity of the organization providing it. While it is also true that > many > other states view ASCP as a valid certifying agency and, ASCP > certification > as a measure of competency, the NYS education department (NYSED) has > chosen > not to accept ASCP certification as a means of credentialing for grand > fathering or as a means to establish competency in the absence or presence > of an examination for any laboratory discipline. However, I believe that > NYSED has selected ASCP to create and administer a "generalist" exam based > on the educational curriculum mandated by the NYSED and the Clinical > Laboratory Technology Practice Act. > > As the law currently stands in NYS, anyone who was not grad fathered will > have to meet the educational requirements AND take the licensing > examination > in order to be employed in NYS (for detail info on the curriculum > material, > please see article 165 of the education law > http://www.op.nysed.gov/clp.htm). > > In NYS, this law applies to all clinical laboratory personnel, including > histologist. NYS does not recognize nor require ASCP certification for > licensing. > > For those of you in other states that are considering proposing a > licensing > law (I think I saw a message thread from a few on this), I suggest that > you > take a look at the NYS law...... > I hope this helps. If you have any questions, please feel free to respond. > Luis > > Luis Chiriboga PhD., HT (ASCP) QIHC > New York University School of Medicine > NYU Cancer Institute IHC Core Facility > Bellevue Hospital Center > Department of Pathology 4W27 > 27th Street & First Avenue > New York, N.Y. 10016 > V (212)562-4667 > F (212)263-2041 > > > Vice President > New York State Histotechnological Society > http://www.nyhisto.org/ > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Michelle > McCoy > Sent: Friday, September 14, 2007 12:01 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] States that require license > > > Someone in a recent previous post had stated that they thought only one > state required state licensing-I know NY and FL do now. I read in an > article > about NY state licensing http://www.medscape.com/viewarticle/542005 > law this excerpt: > > *With one exception (WV), all states requiring licensure (CA, FL, GA, HI, > LA, MT, NV, ND, PR, RI, TN) recognize the American Society for Clinical > Pathology (ASCP) as an approved certifying agency.* > *Commissioner's regulations require candidates to pass a generalist > examination for CLT and CT. With the exception of the grandparenting > period > (through September 1, 2008) specialists without generalist certification > will be required to take a generalist examination to qualify for > licensure.* > ** > -I am hoping to find out if this law applies to Histology as well > -If this is an up to date list of the states that require it, if so > -If grandfathered techs in these states had to take ascp or if they took a > different state test to get the state license. > -Is there any site anyone knows about that keeps up to date information on > what all the different states are doing regarding licensure requirements > in > our field other than sifting through the archives here at histonet? > Thanks for any input- > Michelle > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alaskagirl1950 <@t> yahoo.com Fri Sep 14 14:17:30 2007 From: alaskagirl1950 <@t> yahoo.com (Patricia Adams) Date: Fri Sep 14 14:17:40 2007 Subject: [Histonet] Histotech vacancies In-Reply-To: <6BFF6D137DF6BC43B33891BA96E83B19BBF478@PGHCR-EXMB-VS-1.na.fshrnet.com> Message-ID: <154089.97099.qm@web52502.mail.re2.yahoo.com> I am one of those who did not have the benefit of courses. I was given the ShSheehannd told to know it forwards and backwards. The poor book never left my hand for a year. My young chchildrent the time worried that I would read from it for their bedtime stories. The day that I received the information that I had passed, my chchildrensked if that meant that the book could be put down. There was so much in the test that I had never heard of. But having read so much about the workings of stains I was able to figure out the best answer. The two that I went with to take the test did not pass. One was a student training in the same Lab as me. The other in a private lab. Those weeks waiting for the results were the longest in my life. (back when it was a manual written test). Patricia Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D ____________________________________________________________________________________ Shape Yahoo! in your own image. Join our Network Research Panel today! http://surveylink.yahoo.com/gmrs/yahoo_panel_invite.asp?a=7 From jlinda <@t> ces.clemson.edu Fri Sep 14 14:21:47 2007 From: jlinda <@t> ces.clemson.edu (Linda Jenkins) Date: Fri Sep 14 14:22:11 2007 Subject: [Histonet] South Carolina meeting Message-ID: <6.2.3.4.2.20070914142458.034ec500@mailhost.ces.clemson.edu> South Carolina will be hosting their fall meeting September 21 - 22 at Pawleys Plantation Golf and Country Club at Pawleys Island, SC. It's not too late to register and villa rooms are still available at the special rate. Dr. Karen Burg will be speaking on "Tissues of the Future" and Peggy Wenk will be speaking on "Control Blocks and Tissues for Histology and Immunology" and "Sectioning Artifacts: Causes and Cures". 5 reasons to attend this meeting: 1. Earn 7 contact hours! 2. Play some incredible golf! 3. Meet a ghost! 4. Buy a hammock! 5. Enjoy free gourmet food! If you would like to receive an electronic copy of the program, please contact Linda Jenkins at . Thanks, Linda Linda Jenkins, HT Clemson University Department of Bioengineering 501 Rhodes Research Center Clemson, SC 29634-0905 http://www.ces.clemson.edu/bio/research/histo.html From Janet.Bonner <@t> FLHOSP.ORG Fri Sep 14 14:31:59 2007 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Sep 14 14:32:53 2007 Subject: [Histonet] I think I will give it an acronym References: <6.0.0.22.1.20070914130106.01aff958@gemini.msu.montana.edu> Message-ID: <5F31F38C96781A4FBE3196EBC22D47802E112B@fhosxchmb006.ADVENTISTCORP.NET> Honestly, they need to start over with a new name! ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Gayle Callis Sent: Fri 9/14/2007 3:07 PM To: curtis.king@thermofisher.com; Histonet@lists.utsouthwestern.edu Subject: [Histonet] I think I will give it an acronym Curtis, I think I will now assign an acronym, TFSci or maybe ThermFishSci. Too long to type out all the time. >Subject: RE: [Histonet] AO 860 sliding microtome and Caroline Bass >Date: Fri, 14 Sep 2007 14:03:12 -0400 > >From: "King, Curtis - RAS" > >Just to clear things up The Company Name is ThermoFisher Scientific. > >Thermo Shandon is a Brand just like Thermo Richard Allen is now a brand. > >Curtis D King, HT (ASCP) >Research and Development >ThermoFisher Scientific >4481 Campus Drive >Kalamazoo, MI 49008 >800-522-7270 ext. 672 >Curtis.King@thermofisher.com > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From godsgalnow <@t> aol.com Fri Sep 14 14:35:48 2007 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Fri Sep 14 14:36:09 2007 Subject: [Histonet] negative controls Message-ID: <8C9C51D962FF31B-284-1FE4@webmail-md17.sysops.aol.com> How many non-CAP labs out there are doing negative controls on every block that you do an antibody on? If you have a case that goes A-J and they are all 1 block each and you do an AE1/AE3 on blocks B,C,F,& J, will you do a negative on every block or just one for the entire case? This has been an ongoing debate with us.? We typincally only do 1 negative control for the case and given the above example, it might be on block A.? We ususally cut an extra on every block we cut and when the IHC is requested we just go and pull it and for the negative, we just grab whatever slide is left to run the negative on.? What is everyone else doing?? Roxanne ________________________________________________________________________ Email and AIM finally together. You've gotta check out free AOL Mail! - http://mail.aol.com From doug <@t> ppspath.com Fri Sep 14 15:42:25 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Fri Sep 14 14:43:00 2007 Subject: [Histonet] I think I will give it an acronym In-Reply-To: <5F31F38C96781A4FBE3196EBC22D47802E112B@fhosxchmb006.ADVENTISTCORP.NET> Message-ID: The acronym is still longer than most company names. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonner, Janet Sent: Friday, September 14, 2007 2:32 PM To: Gayle Callis; curtis.king@thermofisher.com; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] I think I will give it an acronym Honestly, they need to start over with a new name! ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Gayle Callis Sent: Fri 9/14/2007 3:07 PM To: curtis.king@thermofisher.com; Histonet@lists.utsouthwestern.edu Subject: [Histonet] I think I will give it an acronym Curtis, I think I will now assign an acronym, TFSci or maybe ThermFishSci. Too long to type out all the time. >Subject: RE: [Histonet] AO 860 sliding microtome and Caroline Bass >Date: Fri, 14 Sep 2007 14:03:12 -0400 > >From: "King, Curtis - RAS" > >Just to clear things up The Company Name is ThermoFisher Scientific. > >Thermo Shandon is a Brand just like Thermo Richard Allen is now a brand. > >Curtis D King, HT (ASCP) >Research and Development >ThermoFisher Scientific >4481 Campus Drive >Kalamazoo, MI 49008 >800-522-7270 ext. 672 >Curtis.King@thermofisher.com > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From oshel1pe <@t> cmich.edu Fri Sep 14 15:04:43 2007 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Fri Sep 14 15:04:59 2007 Subject: [Histonet] I think I will give it an acronym In-Reply-To: <200709141945.l8EJjTRS031721@cmich.edu> References: <200709141945.l8EJjTRS031721@cmich.edu> Message-ID: How about the Empire? That's what they've become. Phil >The acronym is still longer than most company names. > >Douglas D. Deltour HT(ASCP) >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonner, >Janet >Sent: Friday, September 14, 2007 2:32 PM >To: Gayle Callis; curtis.king@thermofisher.com; >Histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] I think I will give it an acronym > >Honestly, they need to start over with a new name! > >________________________________ > >From: histonet-bounces@lists.utsouthwestern.edu on behalf of Gayle Callis >Sent: Fri 9/14/2007 3:07 PM >To: curtis.king@thermofisher.com; Histonet@lists.utsouthwestern.edu >Subject: [Histonet] I think I will give it an acronym > > > >Curtis, > >I think I will now assign an acronym, TFSci or maybe ThermFishSci. Too >long to type out all the time. > > >>Subject: RE: [Histonet] AO 860 sliding microtome and Caroline Bass >>Date: Fri, 14 Sep 2007 14:03:12 -0400 >> >>From: "King, Curtis - RAS" >> >>Just to clear things up The Company Name is ThermoFisher Scientific. >> >>Thermo Shandon is a Brand just like Thermo Richard Allen is now a brand. >> >>Curtis D King, HT (ASCP) >>Research and Development >>ThermoFisher Scientific >>4481 Campus Drive >>Kalamazoo, MI 49008 >>800-522-7270 ext. 672 >>Curtis.King@thermofisher.com >> >> > >Gayle Callis >MT,HT,HTL(ASCP) >Research Histopathology Supervisor >Veterinary Molecular Biology >Montana State University - Bozeman >PO Box 173610 >Bozeman MT 59717-3610 -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 From doug <@t> ppspath.com Fri Sep 14 16:09:49 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Fri Sep 14 15:10:23 2007 Subject: [Histonet] I think I will give it an acronym In-Reply-To: <43227931939CA641907E99F4E9A29532019C807E@PGHCR-EXMB-VS-3.na.fshrnet.com> Message-ID: We have a real acronym, "PPS". Give it another year and I am sure you will have another company to go behind of or in front of that ever growing name of yours. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: King, Curtis - RAS [mailto:curtis.king@thermofisher.com] Sent: Friday, September 14, 2007 2:46 PM To: Douglas D Deltour Subject: RE: [Histonet] I think I will give it an acronym I personally don't see as difficult to say as Professional Pathology Services, PC or lat least just as long. Curtis D King, HT (ASCP) Research and Development ThermoFisher Scientific 4481 Campus Drive Kalamazoo, MI 49008 800-522-7270 ext. 672 Curtis.King@thermofisher.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Friday, September 14, 2007 4:42 PM To: 'Bonner, Janet'; 'Gayle Callis'; King, Curtis - RAS; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] I think I will give it an acronym The acronym is still longer than most company names. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonner, Janet Sent: Friday, September 14, 2007 2:32 PM To: Gayle Callis; curtis.king@thermofisher.com; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] I think I will give it an acronym Honestly, they need to start over with a new name! ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Gayle Callis Sent: Fri 9/14/2007 3:07 PM To: curtis.king@thermofisher.com; Histonet@lists.utsouthwestern.edu Subject: [Histonet] I think I will give it an acronym Curtis, I think I will now assign an acronym, TFSci or maybe ThermFishSci. Too long to type out all the time. >Subject: RE: [Histonet] AO 860 sliding microtome and Caroline Bass >Date: Fri, 14 Sep 2007 14:03:12 -0400 > >From: "King, Curtis - RAS" > >Just to clear things up The Company Name is ThermoFisher Scientific. > >Thermo Shandon is a Brand just like Thermo Richard Allen is now a brand. > >Curtis D King, HT (ASCP) >Research and Development >ThermoFisher Scientific >4481 Campus Drive >Kalamazoo, MI 49008 >800-522-7270 ext. 672 >Curtis.King@thermofisher.com > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Sep 14 15:20:46 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Sep 14 15:20:59 2007 Subject: [Histonet] negative controls In-Reply-To: <8C9C51D962FF31B-284-1FE4@webmail-md17.sysops.aol.com> Message-ID: <705589.47704.qm@web61215.mail.yahoo.com> One negative per block tested! You need to know if any reaction in any block (section) is of positive or a negative (background) nature. Another thing would be the positive control. You need only one positive control, because in that case you are testing the Ab reactivity. Ren? J. godsgalnow@aol.com wrote: How many non-CAP labs out there are doing negative controls on every block that you do an antibody on? If you have a case that goes A-J and they are all 1 block each and you do an AE1/AE3 on blocks B,C,F,& J, will you do a negative on every block or just one for the entire case? This has been an ongoing debate with us.? We typincally only do 1 negative control for the case and given the above example, it might be on block A.? We ususally cut an extra on every block we cut and when the IHC is requested we just go and pull it and for the negative, we just grab whatever slide is left to run the negative on.? What is everyone else doing?? Roxanne ________________________________________________________________________ Email and AIM finally together. You've gotta check out free AOL Mail! - http://mail.aol.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: mail, news, photos & more. From rjbuesa <@t> yahoo.com Fri Sep 14 15:21:48 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Sep 14 15:22:00 2007 Subject: [Histonet] I think I will give it an acronym In-Reply-To: Message-ID: <199783.72255.qm@web61218.mail.yahoo.com> Whose emprire, Leica Microsystems? Ren? J. Philip Oshel wrote: How about the Empire? That's what they've become. Phil --------------------------------- Boardwalk for $500? In 2007? Ha! Play Monopoly Here and Now (it's updated for today's economy) at Yahoo! Games. From oshel1pe <@t> cmich.edu Fri Sep 14 15:28:42 2007 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Fri Sep 14 15:28:58 2007 Subject: [Histonet] I think I will give it an acronym In-Reply-To: <199783.72255.qm@web61218.mail.yahoo.com> References: <199783.72255.qm@web61218.mail.yahoo.com> Message-ID: Thermo. Leica is owned by a holding company, but I forget the name. Phil Whose emprire, Leica Microsystems? Ren? J. Philip Oshel wrote: How about the Empire? That's what they've become. Phil -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 From JMacDonald <@t> mtsac.edu Fri Sep 14 15:33:41 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Sep 14 15:33:56 2007 Subject: [Histonet] My experiences with HT vacancies In-Reply-To: Message-ID: Just to clarify: It is NAACLS (National Accrediting Agency for Clinical Laboratory Sciences) that accredits programs. NCCLS (National Commission for Clinical Laboratory Standards) has been replaced by CLSI. Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu "Orr, Rebecca" Sent by: histonet-bounces@lists.utsouthwestern.edu 09/14/2007 05:59 AM To cc Subject [Histonet] My experiences with HT vacancies Cripes I wrote a BOOK! I don't have enough to do today. Actually I do, I'm just avoiding it. So if anyone is really interested enough to read this through, and there's anything I can do to help, just email me. Our experience with online training has been very good. First of all I attended one of Peggy Wenk's seminars on starting your own training program, it was very informative and Peggy was very helpful in the follow up with my questions and issues. THANKS PEGGY!!!! We had to get NCCLS accreditation so that we could affiliate with the college that was offering the online classes. We already had a set up in place, since our laboratory also has MT training school. We worked closely with the Harford College to attain this. We had our HR change some job descriptions around so that we had some "flex" positions. So if we had openings and we had a qualified tech to fill it, they could be hired as such. We were able to flex two positions so that if there wasn't a tech available we could fill it with a student. When this occurred we designated these positions as Histology Trainee. These students were hired as full time staff and their tuition was paid via our tuition re-imbursement program. (We get up to 5k/year here). We were able to arrange that the students sign a commitment of 2 years after they finished the online course. They were expected to pass the HT exam within a period of time (I can't remember what we decided). IF they chose to leave employment before the 2 years, all they had to do was refund their tuition. It has been a great experience for our lab and rejuvenated our morale. We have two bright kids who transferred over from clerical positions. One of them had to take a couple of night school classes to fulfill the requirements for the college, but we couldn't be more pleased with the results. We have a staff of more senior (ok ok OLD! Me included) who have a ton of experience we're just waiting to share with these students. I think for my staff, they have a great sense of satisfaction and I have been quite surprised at their ability to teach, something they haven't had a chance to do. I am very glad the staff here has a commitment to this as well...I think you need their "buy in" in order for these students to get the best training. I do monitor their progress and review all their assignments. They complete modules and I check it over and then add my additional questions for the students. I am working on a more formal Syllabus for us to use and am learning as the students and I progress. They are learning how to be histo techs and I'm learning how to teach them. For example, they had an assignment about the different kinds of microscopes. I asked additional questions around the use of the microscopes, what type of special stains required a fluorescent scope, what's the difference between resolution and magnification, what is refractive index, plastic tape versus glass coverslip...and I had them identify the parts of the 'scope...I ended up with about 30- 50 more in depth questions to complement their training. We have a fluorescent scope, dissecting scope,etc, and an ancient GIANT EM scope...so we went on a "field trip" to look at the scopes and use them....They have a contact person at the college and they keep in touch with her on a weekly basis. They have assignments to finish on their own pace, but I keep them on a steady schedule so they will finish everything in the 10 month period. We give them time during the day to do assignments, but they do spend the greater part of their day working with the Tech's. Whew! HAPPY FRIDAY! Becky Orr CLA,HT(ASCP)QIHC Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Fri Sep 14 16:35:49 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Fri Sep 14 15:36:23 2007 Subject: {SPAM?} RE: [Histonet] I think I will give it an acronym In-Reply-To: Message-ID: <1022316947-167949280@pathology.swmed.edu> Danaher Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Philip Oshel Sent: Friday, September 14, 2007 3:29 PM To: Histonet@Pathology.swmed.edu Subject: {SPAM?} RE: [Histonet] I think I will give it an acronym Thermo. Leica is owned by a holding company, but I forget the name. Phil Whose emprire, Leica Microsystems? Ren? J. Philip Oshel wrote: How about the Empire? That's what they've become. Phil -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Fri Sep 14 15:39:08 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Sep 14 15:39:17 2007 Subject: [Histonet] I think I will give it an acronym In-Reply-To: <6.0.0.22.1.20070914130106.01aff958@gemini.msu.montana.edu> Message-ID: <000001c7f70f$4f79a210$d00f7ca5@lurie.northwestern.edu> Not to mention UpstateMilliporeLabvision all tied to Fisher etc! Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Friday, September 14, 2007 2:07 PM To: curtis.king@thermofisher.com; Histonet@lists.utsouthwestern.edu Subject: [Histonet] I think I will give it an acronym Curtis, I think I will now assign an acronym, TFSci or maybe ThermFishSci. Too long to type out all the time. >Subject: RE: [Histonet] AO 860 sliding microtome and Caroline Bass >Date: Fri, 14 Sep 2007 14:03:12 -0400 > >From: "King, Curtis - RAS" > >Just to clear things up The Company Name is ThermoFisher Scientific. > >Thermo Shandon is a Brand just like Thermo Richard Allen is now a brand. > >Curtis D King, HT (ASCP) >Research and Development >ThermoFisher Scientific >4481 Campus Drive >Kalamazoo, MI 49008 >800-522-7270 ext. 672 >Curtis.King@thermofisher.com > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Sep 14 15:41:22 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Sep 14 15:41:34 2007 Subject: [Histonet] I think I will give it an acronym In-Reply-To: <000001c7f70f$4f79a210$d00f7ca5@lurie.northwestern.edu> Message-ID: <357380.60593.qm@web61223.mail.yahoo.com> Which is the compiting enterprise's acronym? Ren? J. Bernice Frederick wrote: Not to mention UpstateMilliporeLabvision all tied to Fisher etc! Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Friday, September 14, 2007 2:07 PM To: curtis.king@thermofisher.com; Histonet@lists.utsouthwestern.edu Subject: [Histonet] I think I will give it an acronym Curtis, I think I will now assign an acronym, TFSci or maybe ThermFishSci. Too long to type out all the time. >Subject: RE: [Histonet] AO 860 sliding microtome and Caroline Bass >Date: Fri, 14 Sep 2007 14:03:12 -0400 > >From: "King, Curtis - RAS" > >Just to clear things up The Company Name is ThermoFisher Scientific. > >Thermo Shandon is a Brand just like Thermo Richard Allen is now a brand. > >Curtis D King, HT (ASCP) >Research and Development >ThermoFisher Scientific >4481 Campus Drive >Kalamazoo, MI 49008 >800-522-7270 ext. 672 >Curtis.King@thermofisher.com > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Catch up on fall's hot new shows on Yahoo! TV. Watch previews, get listings, and more! From b-frederick <@t> northwestern.edu Fri Sep 14 15:41:43 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Sep 14 15:41:53 2007 Subject: [Histonet] I think I will give it an acronym In-Reply-To: Message-ID: <000201c7f70f$abff16a0$d00f7ca5@lurie.northwestern.edu> One on many. Leica now has vision biosystems! And they were AO long, long ago! Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Philip Oshel Sent: Friday, September 14, 2007 3:05 PM To: Histonet@Pathology.swmed.edu Subject: RE: [Histonet] I think I will give it an acronym How about the Empire? That's what they've become. Phil >The acronym is still longer than most company names. > >Douglas D. Deltour HT(ASCP) >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonner, >Janet >Sent: Friday, September 14, 2007 2:32 PM >To: Gayle Callis; curtis.king@thermofisher.com; >Histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] I think I will give it an acronym > >Honestly, they need to start over with a new name! > >________________________________ > >From: histonet-bounces@lists.utsouthwestern.edu on behalf of Gayle Callis >Sent: Fri 9/14/2007 3:07 PM >To: curtis.king@thermofisher.com; Histonet@lists.utsouthwestern.edu >Subject: [Histonet] I think I will give it an acronym > > > >Curtis, > >I think I will now assign an acronym, TFSci or maybe ThermFishSci. Too >long to type out all the time. > > >>Subject: RE: [Histonet] AO 860 sliding microtome and Caroline Bass >>Date: Fri, 14 Sep 2007 14:03:12 -0400 >> >>From: "King, Curtis - RAS" >> >>Just to clear things up The Company Name is ThermoFisher Scientific. >> >>Thermo Shandon is a Brand just like Thermo Richard Allen is now a brand. >> >>Curtis D King, HT (ASCP) >>Research and Development >>ThermoFisher Scientific >>4481 Campus Drive >>Kalamazoo, MI 49008 >>800-522-7270 ext. 672 >>Curtis.King@thermofisher.com >> >> > >Gayle Callis >MT,HT,HTL(ASCP) >Research Histopathology Supervisor >Veterinary Molecular Biology >Montana State University - Bozeman >PO Box 173610 >Bozeman MT 59717-3610 -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Sep 14 16:00:08 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Sep 14 16:00:21 2007 Subject: [Histonet] I think I will give it an acronym In-Reply-To: <000201c7f70f$abff16a0$d00f7ca5@lurie.northwestern.edu> Message-ID: <517393.84936.qm@web61218.mail.yahoo.com> If I recall correctly, Leica (once named Ernst Leitz, Wetzlar) bought the microscopes and microtomes sections of Carl Reichert (Austria), Wild-Heerburg (Switzerland), American Optical and Bausch and Lomb (USA). After Vision ByoSystems (Australia) grew enough to buy NovoCastra (UK), it was bought by Leica after a failed hostile takeover by Ventana. Now Leica Microsystems can offer all types of consumables, slides and cassettes printers, sells the Peloris tissue processors and the Bond autostainers, manufactures/sells embedding stations, micrtotomes, microscopes, cryostats all sorts of histology automated instruments and has even developed a type of microscope (I call it "Cyclops" because it does not carry "eyes" = eyepieces and is designed just for digital imaging). It is also manufacturing very cheap (and not very good microscopes) in China (any surprises there?). At this moment in Europe only Carl Zeiss (Germany) and Lomo (Russia still manufacturing variations of 1937 Zeiss microscopes) and some companies in Russia (manufacturing copies of the horizontal OmE Reichert microtomes and copies of the Autotechnicon) can be considered as independent from Leica. Is that Thermo larger than Leica Microsystems? Ren? J. Bernice Frederick wrote: One on many. Leica now has vision biosystems! And they were AO long, long ago! Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Philip Oshel Sent: Friday, September 14, 2007 3:05 PM To: Histonet@Pathology.swmed.edu Subject: RE: [Histonet] I think I will give it an acronym How about the Empire? That's what they've become. Phil >The acronym is still longer than most company names. > >Douglas D. Deltour HT(ASCP) >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonner, >Janet >Sent: Friday, September 14, 2007 2:32 PM >To: Gayle Callis; curtis.king@thermofisher.com; >Histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] I think I will give it an acronym > >Honestly, they need to start over with a new name! > >________________________________ > >From: histonet-bounces@lists.utsouthwestern.edu on behalf of Gayle Callis >Sent: Fri 9/14/2007 3:07 PM >To: curtis.king@thermofisher.com; Histonet@lists.utsouthwestern.edu >Subject: [Histonet] I think I will give it an acronym > > > >Curtis, > >I think I will now assign an acronym, TFSci or maybe ThermFishSci. Too >long to type out all the time. > > >>Subject: RE: [Histonet] AO 860 sliding microtome and Caroline Bass >>Date: Fri, 14 Sep 2007 14:03:12 -0400 >> >>From: "King, Curtis - RAS" >> >>Just to clear things up The Company Name is ThermoFisher Scientific. >> >>Thermo Shandon is a Brand just like Thermo Richard Allen is now a brand. >> >>Curtis D King, HT (ASCP) >>Research and Development >>ThermoFisher Scientific >>4481 Campus Drive >>Kalamazoo, MI 49008 >>800-522-7270 ext. 672 >>Curtis.King@thermofisher.com >> >> > >Gayle Callis >MT,HT,HTL(ASCP) >Research Histopathology Supervisor >Veterinary Molecular Biology >Montana State University - Bozeman >PO Box 173610 >Bozeman MT 59717-3610 -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Check out the hottest 2008 models today at Yahoo! Autos. From hhawkins <@t> utmb.edu Fri Sep 14 16:15:42 2007 From: hhawkins <@t> utmb.edu (Hawkins, Hal K.) Date: Fri Sep 14 16:16:06 2007 Subject: [Histonet] I think I will give it an acronym In-Reply-To: <000201c7f70f$abff16a0$d00f7ca5@lurie.northwestern.edu> Message-ID: FYI http://www.fundinguniverse.com/company-histories/Leica-Microsystems-Hold ings-GmbH-Company-History.html -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Friday, September 14, 2007 3:42 PM To: 'Philip Oshel'; Histonet@Pathology.swmed.edu Subject: RE: [Histonet] I think I will give it an acronym One on many. Leica now has vision biosystems! And they were AO long, long ago! Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Philip Oshel Sent: Friday, September 14, 2007 3:05 PM To: Histonet@Pathology.swmed.edu Subject: RE: [Histonet] I think I will give it an acronym How about the Empire? That's what they've become. Phil >The acronym is still longer than most company names. > >Douglas D. Deltour HT(ASCP) >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonner, >Janet >Sent: Friday, September 14, 2007 2:32 PM >To: Gayle Callis; curtis.king@thermofisher.com; >Histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] I think I will give it an acronym > >Honestly, they need to start over with a new name! > >________________________________ > >From: histonet-bounces@lists.utsouthwestern.edu on behalf of Gayle Callis >Sent: Fri 9/14/2007 3:07 PM >To: curtis.king@thermofisher.com; Histonet@lists.utsouthwestern.edu >Subject: [Histonet] I think I will give it an acronym > > > >Curtis, > >I think I will now assign an acronym, TFSci or maybe ThermFishSci. Too >long to type out all the time. > > >>Subject: RE: [Histonet] AO 860 sliding microtome and Caroline Bass >>Date: Fri, 14 Sep 2007 14:03:12 -0400 >> >>From: "King, Curtis - RAS" >> >>Just to clear things up The Company Name is ThermoFisher Scientific. >> >>Thermo Shandon is a Brand just like Thermo Richard Allen is now a brand. >> >>Curtis D King, HT (ASCP) >>Research and Development >>ThermoFisher Scientific >>4481 Campus Drive >>Kalamazoo, MI 49008 >>800-522-7270 ext. 672 >>Curtis.King@thermofisher.com >> >> > >Gayle Callis >MT,HT,HTL(ASCP) >Research Histopathology Supervisor >Veterinary Molecular Biology >Montana State University - Bozeman >PO Box 173610 >Bozeman MT 59717-3610 -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Fri Sep 14 18:05:50 2007 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Fri Sep 14 18:06:01 2007 Subject: [Histonet] McKesson Horizon Lab for pathology Message-ID: I'm working at a rural hospital, part of a local hospital group all of which use McKesson's Horizon Laboratory information system. They're currently developing an anatomic pathology module for it - in March 2007 McKesson's Web site announced that >>Horizon Anatomic Pathology is integrated with the Horizon Lab? laboratory information system (LIS) and is built on the Horizon Architecture?, McKesson's next-generation enterprise-wide common platform and architecture.<< Does any of you have this anatomic pathology application - assuming it's even available - or is your hospital interfacing any other pathology information system (such as CoPath or PowerPath) with McKesson's Horizon LIS? This little rural hospital is desperately in need of a pathology information system. Bob Richmond Samurai Pathologist Knoxville TN From LuckG <@t> empirehealth.org Fri Sep 14 20:01:30 2007 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Fri Sep 14 20:01:45 2007 Subject: [Histonet] negative controls In-Reply-To: <705589.47704.qm@web61215.mail.yahoo.com> Message-ID: <6BB8BC4519AAB844B174FC739A679BBCCEFEB4@IRMEXCH01.irm.inhs.org> Hello all, We do one negative control for each block as well, which I believe in general is good practice. However refer to the discussion which follows the "ANP.22570" question of the 12/12/06 edition of the CAP Anatomic Pathology checklist. This specifically relates to the example which Roxanne has illustrated in her question. Take special note of the final sentence of that discussion if you make the change from one neg. for every block. Greg Luck, B.S., HT(ASCP) Anatomic Pathology Supervisor Deaconess Med Cntr 800 W. 5th Ave Spokane, WA 99204 Offc 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconessmc.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, September 14, 2007 1:21 PM To: godsgalnow@aol.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] negative controls One negative per block tested! You need to know if any reaction in any block (section) is of positive or a negative (background) nature. Another thing would be the positive control. You need only one positive control, because in that case you are testing the Ab reactivity. Ren? J. godsgalnow@aol.com wrote: How many non-CAP labs out there are doing negative controls on every block that you do an antibody on? If you have a case that goes A-J and they are all 1 block each and you do an AE1/AE3 on blocks B,C,F,& J, will you do a negative on every block or just one for the entire case? This has been an ongoing debate with us.? We typincally only do 1 negative control for the case and given the above example, it might be on block A.? We ususally cut an extra on every block we cut and when the IHC is requested we just go and pull it and for the negative, we just grab whatever slide is left to run the negative on.? What is everyone else doing?? Roxanne ________________________________________________________________________ Email and AIM finally together. You've gotta check out free AOL Mail! - http://mail.aol.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: mail, news, photos & more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kemlo <@t> f2s.com Sat Sep 15 10:47:58 2007 From: kemlo <@t> f2s.com (kemlo) Date: Sat Sep 15 10:48:35 2007 Subject: [Histonet] HPV testing In-Reply-To: <939852.53307.qm@web32015.mail.mud.yahoo.com> References: <939852.53307.qm@web32015.mail.mud.yahoo.com> Message-ID: <5859AA46D71A40548A6CE1EF9DB6ED21@KemloPC> PCR method for HPV, is best. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of audra bilbo Sent: 14 September 2007 15:21 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HPV testing What is the best method for Cytology HPV testing? We run both Thin Prep and SurePath specimens. Audra Bilbo Texarkana, TX ____________________________________________________________________________ ________ Luggage? GPS? Comic books? Check out fitting gifts for grads at Yahoo! Search http://search.yahoo.com/search?fr=oni_on_mail&p=graduation+gifts&cs=bz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TeresaJHarris <@t> msn.com Sat Sep 15 16:10:26 2007 From: TeresaJHarris <@t> msn.com (TeresaJHarris@msn.com) Date: Sat Sep 15 16:10:46 2007 Subject: [Histonet] IHC Forum at NSH, 2007 Message-ID: Happy Fall All, It is that time of year again and preparations are being made for the NSH symposium/convention 2007 in Denver. I am attempting to get funding to attend anyway. My question is in regard to the IHC forum at the NSH convention. It sounds wonderful and loaded with valuable information. I want to attend the forum for the great price of $115. I would also like to get a Certificate of Completion in Immunohistochemistry. Do I need to take three more halfday workshops in addition to the forum to receive certification? What category does the forum fall into? Keeping my fingers crossed for funding approval, (September 28 is the day I will find out) and getting that certification. That paraffin hot tub idea was very cute. The large paraffin melting pot. Where else but the USA? God Bless America and our troops. Teresa From amosbrooks <@t> gmail.com Sat Sep 15 22:16:23 2007 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sat Sep 15 22:16:33 2007 Subject: [Histonet] Re: negative controls Message-ID: <582736990709152016xf425442ne92ab1589a5aac91@mail.gmail.com> Hypothetically: Let's say an endometriosis case has several parts as they commonly do. A) Uterus bx (obviously), B) Ovary, C) Lg Colon bx, D) Kidney E) Liver Bx. So an IHC is ordered on the case and randomly part A is chosen as a representative negative control. Have you ever noticed how much endogenous biotin is in kidney & liver? Since there is not a negative control on it there is no real way of knowing that the labeling that one would see there is not real. Honestly having a negative on each block is best, if not for eash test being run (a tall order for sure!). Before polymer detection became popular I once used kidney as a positive control for CD10. It labels the brush boarder of the proximal convuluded tubule really nicely. Problem is that this is also a great place to find endogenous biotin! This really sold me on the use of negative controls. Thanks Mary! Have a nice day, Amos Message: 1 Date: Fri, 14 Sep 2007 15:35:48 -0400 From: godsgalnow@aol.com Subject: [Histonet] negative controls To: histonet@lists.utsouthwestern.edu Message-ID: <8C9C51D962FF31B-284-1FE4@webmail-md17.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" How many non-CAP labs out there are doing negative controls on every block that you do an antibody on? If you have a case that goes A-J and they are all 1 block each and you do an AE1/AE3 on blocks B,C,F,& J, will you do a negative on every block or just one for the entire case? This has been an ongoing debate with us.? We typincally only do 1 negative control for the case and given the above example, it might be on block A.? We ususally cut an extra on every block we cut and when the IHC is requested we just go and pull it and for the negative, we just grab whatever slide is left to run the negative on.? What is everyone else doing?? Roxanne From jnocito <@t> satx.rr.com Sun Sep 16 09:15:35 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Sun Sep 16 09:15:46 2007 Subject: [Histonet] not histology related Message-ID: <000801c7f86c$0d251940$0202a8c0@yourxhtr8hvc4p> calling all you cooks. bakers, and chemistry people, I'm making peach cobbler. When I'm making pies or some goody that requires pastry, all my recipes have 2 cups flour and 3-4 tablespoons of ice water. My question is: do you use 3-4 TSB of water? This morning, I used almost 1/2 cup. As long as I've been baking, I've never used 3-4 TSB of water. I don't know why this bothers me, but it does. Sometimes my crust is too dry, other times, it's soggy. What is a histotech to do? Thanks y'all JTT From conniegrubaugh <@t> hotmail.com Sun Sep 16 10:33:33 2007 From: conniegrubaugh <@t> hotmail.com (connie grubaugh) Date: Sun Sep 16 10:33:47 2007 Subject: [Histonet] not histology related In-Reply-To: <000801c7f86c$0d251940$0202a8c0@yourxhtr8hvc4p> References: <000801c7f86c$0d251940$0202a8c0@yourxhtr8hvc4p> Message-ID: Joe, I need a little more information. 1. please submit the following, a. the temperature chart of the kitchen before and after you do the cooking. along with the calibration info from the precise calibrated thermometers that you are using and the lot number. b. the temperature of the water and the lot number from the local water dept. c. the lot number of where ever the peaches came from (from outside on a tree the lot number from your local nursery or from where ever you got them if fresh) or the lot number of the can and what grocery store you received them from and the manager of the stores mothers maiden name. d. the counter disinfectant's chart and what type of cleanser you are using. e. the lot number and QC chart from the baking pan or whatever you are using there, f. the calibrating information of the cooking utensils that you are using. g. if this procedure requires eggs or milk or anything else I need the same regulation charts on all of this. h. if milk please locate the cow! i . if chickens please locate the chicken and all the infor related to that, j. if butter or margarine is used please submit all the qc on those products. k. almost forgot I need all the info from your oven too. you know the drill And last but not least please fed ex this infor along with the peach cobbler so I can give you a full assesment of the product. I will post this to the histonet only after you get a pin number to see the results. If I have missed anything just include it in the packet and I'm sure I can use it in my evaluation. Thanks again Joe will be looking forward to the package tomorrow. Connie G. > From: jnocito@satx.rr.com > To: histonet@pathology.swmed.edu > Date: Sun, 16 Sep 2007 09:15:35 -0500 > CC: > Subject: [Histonet] not histology related > > calling all you cooks. bakers, and chemistry people, > I'm making peach cobbler. When I'm making pies or some goody that requires pastry, all my recipes have 2 cups flour and 3-4 tablespoons of ice water. My question is: do you use 3-4 TSB of water? This morning, I used almost 1/2 cup. As long as I've been baking, I've never used 3-4 TSB of water. I don't know why this bothers me, but it does. Sometimes my crust is too dry, other times, it's soggy. What is a histotech to do? Thanks y'all > > JTT > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ More photos; more messages; more whatever ? Get MORE with Windows Live? Hotmail?. NOW with 5GB storage. http://imagine-windowslive.com/hotmail/?locale=en-us&ocid=TXT_TAGHM_migration_HM_mini_5G_0907 From rjbuesa <@t> yahoo.com Sun Sep 16 11:19:20 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Sep 16 11:19:39 2007 Subject: [Histonet] not histology related In-Reply-To: Message-ID: <854103.81268.qm@web61223.mail.yahoo.com> And the pages numbers of the SOP (signed) where the procedure is described, along with the references used. Ren? J. connie grubaugh wrote: Joe, I need a little more information. 1. please submit the following, a. the temperature chart of the kitchen before and after you do the cooking. along with the calibration info from the precise calibrated thermometers that you are using and the lot number. b. the temperature of the water and the lot number from the local water dept. c. the lot number of where ever the peaches came from (from outside on a tree the lot number from your local nursery or from where ever you got them if fresh) or the lot number of the can and what grocery store you received them from and the manager of the stores mothers maiden name. d. the counter disinfectant's chart and what type of cleanser you are using. e. the lot number and QC chart from the baking pan or whatever you are using there, f. the calibrating information of the cooking utensils that you are using. g. if this procedure requires eggs or milk or anything else I need the same regulation charts on all of this. h. if milk please locate the cow! i . if chickens please locate the chicken and all the infor related to that, j. if butter or margarine is used please submit all the qc on those products. k. almost forgot I need all the info from your oven too. you know the drill And last but not least please fed ex this infor along with the peach cobbler so I can give you a full assesment of the product. I will post this to the histonet only after you get a pin number to see the results. If I have missed anything just include it in the packet and I'm sure I can use it in my evaluation. Thanks again Joe will be looking forward to the package tomorrow. Connie G. > From: jnocito@satx.rr.com > To: histonet@pathology.swmed.edu > Date: Sun, 16 Sep 2007 09:15:35 -0500 > CC: > Subject: [Histonet] not histology related > > calling all you cooks. bakers, and chemistry people, > I'm making peach cobbler. When I'm making pies or some goody that requires pastry, all my recipes have 2 cups flour and 3-4 tablespoons of ice water. My question is: do you use 3-4 TSB of water? This morning, I used almost 1/2 cup. As long as I've been baking, I've never used 3-4 TSB of water. I don't know why this bothers me, but it does. Sometimes my crust is too dry, other times, it's soggy. What is a histotech to do? Thanks y'all > > JTT > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ More photos; more messages; more whatever ? Get MORE with Windows Live? Hotmail?. NOW with 5GB storage. http://imagine-windowslive.com/hotmail/?locale=en-us&ocid=TXT_TAGHM_migration_HM_mini_5G_0907_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Shape Yahoo! in your own image. Join our Network Research Panel today! From pruegg <@t> ihctech.net Sun Sep 16 12:04:28 2007 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Sun Sep 16 12:01:14 2007 Subject: [Histonet] NSH IHC Forum Questions Message-ID: <200709161700.l8GH0hS8018268@pro12.abac.com> This is a call for IHC questions to be discussed at the NSH S/C in Denver in OCT. If you have any burning questions you would like our panel of 9 to address please email them to me ahead of time. Thank you, Patsy Ruegg Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net From lpwenk <@t> sbcglobal.net Sun Sep 16 13:17:01 2007 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sun Sep 16 13:17:30 2007 Subject: [Histonet] not histology related In-Reply-To: <000801c7f86c$0d251940$0202a8c0@yourxhtr8hvc4p> Message-ID: <000701c7f88d$c704b480$0202a8c0@HPPav2> Cooking is a lot like doing histology - you tweak a "recipe" until it comes out right for you, in your location, the way you do it, at that time. Due to variables such as: - 2 C. flour may be different amounts of flour, depending upon the density of the flour and how much it settled, and how much water it has absorbed. Some people weigh the flour, rather than use cup measuring devices. - are you using cold or room temperature water? Room temperature or refriderator temperature butter/margarine/lard? - what's the room temperature? (which ultimately means how warm is the butter/margarine/lard) - how much have you handled the dough? That influences the gluten-icity and the temperature. - are you adding just enough water until the dough just forms a ball? Or are you being a little impatient, and keep adding water to make it stick before it's time? - how fine are you cutting in the butter/etc? On the whole, then I make pies (sorry, never made cobblers, but the dough is similar), 2-3 Tbs. of ice cold water for 2 C. flour sounds about right. So, think in terms of doing a Masson trichrome - how long you leave the slides/tissue in each of the dyes depends upon how it was fixed, how it was post-fixed, how strong the dyes are, what the room temperature, whether the solutions were refrigerator temperture or room temperature, the patient's tissue itself, and ultimately the skill, knowledge and patience of the person doing the procedure. Cooking is very similar. Ada Feldman has a great workshop, where she compares histology procedures (fixation, processing, stains) to cooking. After all, protein is protein, lipids are lipids. And if you have ever watched Good Eats with Alton Brown on the Food Network, he uses hokey props to explain the chemistry of food and cooking (and digestion). I love the show, and I'm someone who doesn't have the patience to sit through cooking shows. Here's Alton's recipe for rhubarb peach cobbler, and he uses about 3 TBS ice cold water to about 2 C flour. http://www.foodnetwork.com/food/recipes/recipe/0,,FOOD_9936_31536,00.html?rs rc=search Let us know what your research finds out. Peggy A. Wenk A reluctant cook -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Sunday, September 16, 2007 10:16 AM To: histonet Subject: [Histonet] not histology related calling all you cooks. bakers, and chemistry people, I'm making peach cobbler. When I'm making pies or some goody that requires pastry, all my recipes have 2 cups flour and 3-4 tablespoons of ice water. My question is: do you use 3-4 TSB of water? This morning, I used almost 1/2 cup. As long as I've been baking, I've never used 3-4 TSB of water. I don't know why this bothers me, but it does. Sometimes my crust is too dry, other times, it's soggy. What is a histotech to do? Thanks y'all JTT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie <@t> conxis.com Sun Sep 16 20:08:16 2007 From: laurie <@t> conxis.com (laurie@conxis.com) Date: Sun Sep 16 20:08:34 2007 Subject: [Histonet] Re: not histology related Message-ID: Per Alton Brown and any other cook that I have found to be amusing on Food Network, crust is supposed to be primarily flour and shortening ( insert lard depending on where you are at....) and a little bit of ice water to chill the crust so that it comes together and is nice and flaky when it comes out of the fridge and is rolled out.... personally right now it's apple season here in Minnesota. SOP for crust making can be found in Better Homes and Gardens or anywhere on foodtv.com ;-) happy Sunday.... Laurie Popp From moran.elish <@t> gmail.com Mon Sep 17 04:08:31 2007 From: moran.elish <@t> gmail.com (Moran Elishmereni) Date: Mon Sep 17 04:08:45 2007 Subject: [Histonet] air bubbles seen after mounting with entellan Message-ID: <4a722ef70709170208p3dd48481j25f96c00196dfed9@mail.gmail.com> Dear Histonetters, I am getting a lot of air bubbles after coversliping my slides with entellan (following neo-clear, a xylene substitute). I made sure that the slides are wet with the neo-clear, and then applied the mounting media. Why is this happening? I have to add that I used cold entellan once and this didn't happen, but when the entellan was in room temp (and therefore less viscous), it did. Does viscosity have anything to do with it? I'd appreciate any piece of advice on how to avoid these air bubbles! Many thanks, Moran Elishmereni Department of Pharmacology and Experimental Therapeutics School of Pharmacy, Faculty of Medicine The Hebrew University of Jerusalem POB 12065 Jerusalem 91120, ISRAEL Tel: 972-2-675-8746 Fax: 972-2-675-8144 Email: moran.elish@gmail.com From Kemlo.Rogerson <@t> waht.swest.nhs.uk Mon Sep 17 04:19:53 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Sep 17 04:20:01 2007 Subject: [Histonet] air bubbles seen after mounting with entellan Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222ED04@wahtntex2.waht.swest.nhs.uk> Are the bubbles there at coverslipping or sometime after? You could try degassing the medium under vacuum. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; The world breaks everyone, and afterward, some are strong at the broken places. --Ernest Hemingway This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From HoustonR <@t> chi.osu.edu Mon Sep 17 07:15:05 2007 From: HoustonR <@t> chi.osu.edu (Houston, Ronald) Date: Mon Sep 17 07:15:50 2007 Subject: [Histonet] HPV testing In-Reply-To: <5859AA46D71A40548A6CE1EF9DB6ED21@KemloPC> Message-ID: <979FF5962E234F45B06CF0DB7C1AABB2120270F7@chi2k3ms01.columbuschildrens.net> I believe the "gold standard" at present is the hybrid capture (Digene II) although Roche has a new test out (or coming out) that is reported to be more specific and sensitive Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Columbus Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 houstonr@chi.osu.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kemlo Sent: Saturday, September 15, 2007 11:48 AM To: 'audra bilbo'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HPV testing PCR method for HPV, is best. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of audra bilbo Sent: 14 September 2007 15:21 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HPV testing What is the best method for Cytology HPV testing? We run both Thin Prep and SurePath specimens. Audra Bilbo Texarkana, TX ________________________________________________________________________ ____ ________ Luggage? GPS? Comic books? Check out fitting gifts for grads at Yahoo! Search http://search.yahoo.com/search?fr=oni_on_mail&p=graduation+gifts&cs=bz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From godsgalnow <@t> aol.com Mon Sep 17 07:23:25 2007 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Mon Sep 17 07:24:04 2007 Subject: [Histonet] Re: negative controls In-Reply-To: <582736990709152016xf425442ne92ab1589a5aac91@mail.gmail.com> References: <582736990709152016xf425442ne92ab1589a5aac91@mail.gmail.com> Message-ID: <8C9C73CAE3D237F-9AC-55E@FWM-D09.sysops.aol.com> But what if all of the blocks are prostate core biopsies? Roxanne -----Original Message----- From: Amos Brooks To: histonet@lists.utsouthwestern.edu Sent: Sat, 15 Sep 2007 11:16 pm Subject: [Histonet] Re: negative controls Hypothetically: Let's say an endometriosis case has several parts as they commonly do. A) Uterus bx (obviously), B) Ovary, C) Lg Colon bx, D) Kidney E) Liver Bx. So an IHC is ordered on the case and randomly part A is chosen as a representative negative control. Have you ever noticed how much endogenous biotin is in kidney & liver? Since there is not a negative control on it there is no real way of knowing that the labeling that one would see there is not real. Honestly having a negative on each block is best, if not for eash test being run (a tall order for sure!). Before polymer detection became popular I once used kidney as a positive control for CD10. It labels the brush boarder of the proximal convuluded tubule really nicely. Problem is that this is also a great place to find endogenous biotin! This really sold me on the use of negative controls. Thanks Mary! Have a nice day, Amos Message: 1 Date: Fri, 14 Sep 2007 15:35:48 -0400 From: godsgalnow@aol.com Subject: [Histonet] negative controls To: histonet@lists.utsouthwestern.edu Message-ID: <8C9C51D962FF31B-284-1FE4@webmail-md17.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" How many non-CAP labs out there are doing negative controls on every block that you do an antibody on? If you have a case that goes A-J and they are all 1 block each and you do an AE1/AE3 on blocks B,C,F,& J, will you do a negative on every block or just one for the entire case? This has been an ongoing debate with us.? We typincally only do 1 negative control for the case and given the above example, it might be on block A.? We ususally cut an extra on every block we cut and when the IHC is requested we just go and pull it and for the negative, we just grab whatever slide is left to run the negative on.? What is everyone else doing?? Roxanne _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ Email and AIM finally together. You've gotta check out free AOL Mail! - http://mail.aol.com From b-frederick <@t> northwestern.edu Mon Sep 17 07:25:18 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon Sep 17 07:25:26 2007 Subject: [Histonet] I think I will give it an acronym In-Reply-To: <357380.60593.qm@web61223.mail.yahoo.com> Message-ID: <00fb01c7f925$d22fe990$d00f7ca5@lurie.northwestern.edu> AO/Reichert/Leica/visionbiosystems (which includes Novacastra). I guess we have an Empire and a Kingdom. Where do we fit Dako/Acis? Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Friday, September 14, 2007 3:41 PM To: Bernice Frederick; 'Gayle Callis'; curtis.king@thermofisher.com; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] I think I will give it an acronym Which is the compiting enterprise's acronym? Ren? J. Bernice Frederick wrote: Not to mention UpstateMilliporeLabvision all tied to Fisher etc! Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Friday, September 14, 2007 2:07 PM To: curtis.king@thermofisher.com; Histonet@lists.utsouthwestern.edu Subject: [Histonet] I think I will give it an acronym Curtis, I think I will now assign an acronym, TFSci or maybe ThermFishSci. Too long to type out all the time. >Subject: RE: [Histonet] AO 860 sliding microtome and Caroline Bass >Date: Fri, 14 Sep 2007 14:03:12 -0400 > >From: "King, Curtis - RAS" > >Just to clear things up The Company Name is ThermoFisher Scientific. > >Thermo Shandon is a Brand just like Thermo Richard Allen is now a brand. > >Curtis D King, HT (ASCP) >Research and Development >ThermoFisher Scientific >4481 Campus Drive >Kalamazoo, MI 49008 >800-522-7270 ext. 672 >Curtis.King@thermofisher.com > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____ Catch up on fall's hot new shows on Yahoo! TV. Watch previews, get listings, and more! From rjbuesa <@t> yahoo.com Mon Sep 17 07:44:27 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Sep 17 07:44:39 2007 Subject: [Histonet] I think I will give it an acronym In-Reply-To: <00fb01c7f925$d22fe990$d00f7ca5@lurie.northwestern.edu> Message-ID: <678730.91435.qm@web61218.mail.yahoo.com> In my original relation (I was made aware of it in another e-mail), Leitz also bought "Cambridge Instruments" from the UK, and another minor English manufacturer as well, before buying AO and B&L. Leitz decided to buy out as many microscopes manufacturers as possible. Ren? J. Bernice Frederick wrote: AO/Reichert/Leica/visionbiosystems (which includes Novacastra). I guess we have an Empire and a Kingdom. Where do we fit Dako/Acis? Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Friday, September 14, 2007 3:41 PM To: Bernice Frederick; 'Gayle Callis'; curtis.king@thermofisher.com; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] I think I will give it an acronym Which is the compiting enterprise's acronym? Ren? J. Bernice Frederick wrote: Not to mention UpstateMilliporeLabvision all tied to Fisher etc! Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Friday, September 14, 2007 2:07 PM To: curtis.king@thermofisher.com; Histonet@lists.utsouthwestern.edu Subject: [Histonet] I think I will give it an acronym Curtis, I think I will now assign an acronym, TFSci or maybe ThermFishSci. Too long to type out all the time. >Subject: RE: [Histonet] AO 860 sliding microtome and Caroline Bass >Date: Fri, 14 Sep 2007 14:03:12 -0400 > >From: "King, Curtis - RAS" > >Just to clear things up The Company Name is ThermoFisher Scientific. > >Thermo Shandon is a Brand just like Thermo Richard Allen is now a brand. > >Curtis D King, HT (ASCP) >Research and Development >ThermoFisher Scientific >4481 Campus Drive >Kalamazoo, MI 49008 >800-522-7270 ext. 672 >Curtis.King@thermofisher.com > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Catch up on fall's hot new shows on Yahoo! TV. Watch previews, get listings, and more! --------------------------------- Check out the hottest 2008 models today at Yahoo! Autos. From HornHV <@t> archildrens.org Mon Sep 17 07:45:17 2007 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Mon Sep 17 07:45:32 2007 Subject: [Histonet] negative controls In-Reply-To: <8C9C51D962FF31B-284-1FE4@webmail-md17.sysops.aol.com> References: <8C9C51D962FF31B-284-1FE4@webmail-md17.sysops.aol.com> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB734A@EMAIL.archildrens.org> We do a negative control on each block and sometimes we do 2. If several antibodies are ordered on one block and their species are different, (i.e. rabbit, mouse) we run one negative mouse and one negative rabbit. Each block should have its own negative control. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: Friday, September 14, 2007 2:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] negative controls How many non-CAP labs out there are doing negative controls on every block that you do an antibody on? If you have a case that goes A-J and they are all 1 block each and you do an AE1/AE3 on blocks B,C,F,& J, will you do a negative on every block or just one for the entire case? This has been an ongoing debate with us.? We typincally only do 1 negative control for the case and given the above example, it might be on block A.? We ususally cut an extra on every block we cut and when the IHC is requested we just go and pull it and for the negative, we just grab whatever slide is left to run the negative on.? What is everyone else doing?? Roxanne ________________________________________________________________________ Email and AIM finally together. You've gotta check out free AOL Mail! - http://mail.aol.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From Joanne.Malinowski <@t> crl.com Mon Sep 17 07:43:11 2007 From: Joanne.Malinowski <@t> crl.com (Malinowski, Joanne O,) Date: Mon Sep 17 07:45:35 2007 Subject: [Histonet] Goldner's Trichrome Message-ID: <3E94391DD24ECE418DCBA5EF74BA29940B39E4@shr-exch1.na01.crl.com> Hello Plastic Histonetters, Can anyone share the latest Goldner's Trichrome for MMA ground sections? Ours turns out monochromatic. If not Goldner's, what is your choice for connective tissue for ground MMA sections? Thank you. Joanne Joanne Malinowski,HT ASCP Plastics Lab Manager, Medical Devices Division Charles River Laboratories Pathology Associates 15 Worman's Mill Court, Suite I Frederick, Maryland 21701 Phone 301-624-2034 Fax 301-663-8994 Email: joanne.malinowski@us.crl.com From oshel1pe <@t> cmich.edu Mon Sep 17 07:45:48 2007 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Mon Sep 17 07:46:08 2007 Subject: [Histonet] I think I will give it an acronym In-Reply-To: <517393.84936.qm@web61218.mail.yahoo.com> References: <517393.84936.qm@web61218.mail.yahoo.com> Message-ID: Maybe we should start a pool on how long before it's all ThermoLeica. Phli If I recall correctly, Leica (once named Ernst Leitz, Wetzlar) bought the microscopes and microtomes sections of Carl Reichert (Austria), Wild-Heerburg (Switzerland), American Optical and Bausch and Lomb (USA). After Vision ByoSystems (Australia) grew enough to buy NovoCastra (UK), it was bought by Leica after a failed hostile takeover by Ventana. Now Leica Microsystems can offer all types of consumables, slides and cassettes printers, sells the Peloris tissue processors and the Bond autostainers, manufactures/sells embedding stations, micrtotomes, microscopes, cryostats all sorts of histology automated instruments and has even developed a type of microscope (I call it "Cyclops" because it does not carry "eyes" = eyepieces and is designed just for digital imaging). It is also manufacturing very cheap (and not very good microscopes) in China (any surprises there?). At this moment in Europe only Carl Zeiss (Germany) and Lomo (Russia still manufacturing variations of 1937 Zeiss microscopes) and some companies in Russia (manufacturing copies of the horizontal OmE Reichert microtomes and copies of the Autotechnicon) can be considered as independent from Leica. Is that Thermo larger than Leica Microsystems? Ren? J. Bernice Frederick wrote: One on many. Leica now has vision biosystems! And they were AO long, long ago! Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Philip Oshel Sent: Friday, September 14, 2007 3:05 PM To: Histonet@Pathology.swmed.edu Subject: RE: [Histonet] I think I will give it an acronym How about the Empire? That's what they've become. Phil >The acronym is still longer than most company names. > >Douglas D. Deltour HT(ASCP) >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonner, >Janet >Sent: Friday, September 14, 2007 2:32 PM >To: Gayle Callis; curtis.king@thermofisher.com; >Histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] I think I will give it an acronym > >Honestly, they need to start over with a new name! > >________________________________ > >From: histonet-bounces@lists.utsouthwestern.edu on behalf of Gayle Callis >Sent: Fri 9/14/2007 3:07 PM >To: curtis.king@thermofisher.com; Histonet@lists.utsouthwestern.edu >Subject: [Histonet] I think I will give it an acronym > > > >Curtis, > >I think I will now assign an acronym, TFSci or maybe ThermFishSci. Too >long to type out all the time. > > >>Subject: RE: [Histonet] AO 860 sliding microtome and Caroline Bass >>Date: Fri, 14 Sep 2007 14:03:12 -0400 >> >>From: "King, Curtis - RAS" >> >>Just to clear things up The Company Name is ThermoFisher Scientific. >> >>Thermo Shandon is a Brand just like Thermo Richard Allen is now a brand. >> >>Curtis D King, HT (ASCP) >>Research and Development >>ThermoFisher Scientific >>4481 Campus Drive >>Kalamazoo, MI 49008 >>800-522-7270 ext. 672 >>Curtis.King@thermofisher.com >> >> > >Gayle Callis >MT,HT,HTL(ASCP) >Research Histopathology Supervisor >Veterinary Molecular Biology >Montana State University - Bozeman >PO Box 173610 >Bozeman MT 59717-3610 -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Check out the hottest 2008 models today at Yahoo! Autos. -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 From b-frederick <@t> northwestern.edu Mon Sep 17 07:47:11 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon Sep 17 07:47:16 2007 Subject: [Histonet] not histology related In-Reply-To: <854103.81268.qm@web61223.mail.yahoo.com> Message-ID: <010e01c7f928$e065e250$d00f7ca5@lurie.northwestern.edu> This sounds like we are still on Friday. Joe, don't forget that if you are using a microwave we need all the specs for that too! Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Sunday, September 16, 2007 11:19 AM To: connie grubaugh; Joe Nocito; histonet Subject: RE: [Histonet] not histology related And the pages numbers of the SOP (signed) where the procedure is described, along with the references used. Ren? J. connie grubaugh wrote: Joe, I need a little more information. 1. please submit the following, a. the temperature chart of the kitchen before and after you do the cooking. along with the calibration info from the precise calibrated thermometers that you are using and the lot number. b. the temperature of the water and the lot number from the local water dept. c. the lot number of where ever the peaches came from (from outside on a tree the lot number from your local nursery or from where ever you got them if fresh) or the lot number of the can and what grocery store you received them from and the manager of the stores mothers maiden name. d. the counter disinfectant's chart and what type of cleanser you are using. e. the lot number and QC chart from the baking pan or whatever you are using there, f. the calibrating information of the cooking utensils that you are using. g. if this procedure requires eggs or milk or anything else I need the same regulation charts on all of this. h. if milk please locate the cow! i . if chickens please locate the chicken and all the infor related to that, j. if butter or margarine is used please submit all the qc on those products. k. almost forgot I need all the info from your oven too. you know the drill And last but not least please fed ex this infor along with the peach cobbler so I can give you a full assesment of the product. I will post this to the histonet only after you get a pin number to see the results. If I have missed anything just include it in the packet and I'm sure I can use it in my evaluation. Thanks again Joe will be looking forward to the package tomorrow. Connie G. > From: jnocito@satx.rr.com > To: histonet@pathology.swmed.edu > Date: Sun, 16 Sep 2007 09:15:35 -0500 > CC: > Subject: [Histonet] not histology related > > calling all you cooks. bakers, and chemistry people, > I'm making peach cobbler. When I'm making pies or some goody that requires pastry, all my recipes have 2 cups flour and 3-4 tablespoons of ice water. My question is: do you use 3-4 TSB of water? This morning, I used almost 1/2 cup. As long as I've been baking, I've never used 3-4 TSB of water. I don't know why this bothers me, but it does. Sometimes my crust is too dry, other times, it's soggy. What is a histotech to do? Thanks y'all > > JTT > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ More photos; more messages; more whatever ? Get MORE with Windows Live? Hotmail?. NOW with 5GB storage. http://imagine-windowslive.com/hotmail/?locale=en-us&ocid=TXT_TAGHM_migratio n_HM_mini_5G_0907_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Shape Yahoo! in your own image. Join our Network Research Panel today! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joanne.Malinowski <@t> crl.com Mon Sep 17 07:50:30 2007 From: Joanne.Malinowski <@t> crl.com (Malinowski, Joanne O,) Date: Mon Sep 17 07:50:43 2007 Subject: [Histonet] Goldner's Trichrome for MMA Ground Sections Message-ID: <3E94391DD24ECE418DCBA5EF74BA29940B39E7@shr-exch1.na01.crl.com> Hello Plastic Histonetters, Can anyone share the latest Goldner's Trichrome for MMA ground sections? Ours turns out monochromatic. If not Goldner's, what is your choice for connective tissue for ground MMA sections? Thank you. Joanne Joanne Malinowski,HT ASCP Plastics Lab Manager, Medical Devices Division Charles River Laboratories Pathology Associates 15 Worman's Mill Court, Suite I Frederick, Maryland 21701 Phone 301-624-2034 Fax 301-663-8994 Email: joanne.malinowski@us.crl.com From simmonsca <@t> upmc.edu Mon Sep 17 07:52:59 2007 From: simmonsca <@t> upmc.edu (Simmons, Christopher) Date: Mon Sep 17 07:53:16 2007 Subject: [Histonet] negative controls In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB734A@EMAIL.archildrens.org> References: <8C9C51D962FF31B-284-1FE4@webmail-md17.sysops.aol.com> <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB734A@EMAIL.archildrens.org> Message-ID: <88D6F512ADC2CB428B90845D2D1388E303A5BB75@1upmc-msx6.acct.upmchs.net> CAP requires a negative control for each test. We utilize a micro-array sausage control that allows our slide count to be reduced as the 16-20 tissue types on each of our 3 control slides validated the 350 immunos we have in our battery. Remember to order a negative control not just with each block or part type, but with additional reflex orders as well. Chris Simmons Lead Technologist UPMC Presbyterian Histology 412.647.7660 desk 13242 pager -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Monday, September 17, 2007 8:45 AM To: godsgalnow@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] negative controls We do a negative control on each block and sometimes we do 2. If several antibodies are ordered on one block and their species are different, (i.e. rabbit, mouse) we run one negative mouse and one negative rabbit. Each block should have its own negative control. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: Friday, September 14, 2007 2:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] negative controls How many non-CAP labs out there are doing negative controls on every block that you do an antibody on? If you have a case that goes A-J and they are all 1 block each and you do an AE1/AE3 on blocks B,C,F,& J, will you do a negative on every block or just one for the entire case? This has been an ongoing debate with us.? We typincally only do 1 negative control for the case and given the above example, it might be on block A.? We ususally cut an extra on every block we cut and when the IHC is requested we just go and pull it and for the negative, we just grab whatever slide is left to run the negative on.? What is everyone else doing?? Roxanne ________________________________________________________________________ Email and AIM finally together. You've gotta check out free AOL Mail! - http://mail.aol.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ ------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From simmonsca <@t> upmc.edu Mon Sep 17 07:56:08 2007 From: simmonsca <@t> upmc.edu (Simmons, Christopher) Date: Mon Sep 17 08:00:11 2007 Subject: [Histonet] not histology related In-Reply-To: <010e01c7f928$e065e250$d00f7ca5@lurie.northwestern.edu> References: <854103.81268.qm@web61223.mail.yahoo.com> <010e01c7f928$e065e250$d00f7ca5@lurie.northwestern.edu> Message-ID: <88D6F512ADC2CB428B90845D2D1388E303A5BB76@1upmc-msx6.acct.upmchs.net> Ok, I got this from my wife..she tells me regardless of the water you use, put a piece of dry penne pasta in the center of the pie when you bake it. It will act as a vent to let the right amount of moisture out of the fruit filling, and as a bonus, the additional moisture in the oven will keep the pie crust as the right flakyness. Just reporting this.... Hope it helps:) Chris Simmons Lead Technologist UPMC Presbyterian Histology 412.647.7660 desk 13242 pager -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Monday, September 17, 2007 8:47 AM To: 'Rene J Buesa'; 'connie grubaugh'; 'Joe Nocito'; 'histonet' Subject: RE: [Histonet] not histology related This sounds like we are still on Friday. Joe, don't forget that if you are using a microwave we need all the specs for that too! Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Sunday, September 16, 2007 11:19 AM To: connie grubaugh; Joe Nocito; histonet Subject: RE: [Histonet] not histology related And the pages numbers of the SOP (signed) where the procedure is described, along with the references used. Ren? J. connie grubaugh wrote: Joe, I need a little more information. 1. please submit the following, a. the temperature chart of the kitchen before and after you do the cooking. along with the calibration info from the precise calibrated thermometers that you are using and the lot number. b. the temperature of the water and the lot number from the local water dept. c. the lot number of where ever the peaches came from (from outside on a tree the lot number from your local nursery or from where ever you got them if fresh) or the lot number of the can and what grocery store you received them from and the manager of the stores mothers maiden name. d. the counter disinfectant's chart and what type of cleanser you are using. e. the lot number and QC chart from the baking pan or whatever you are using there, f. the calibrating information of the cooking utensils that you are using. g. if this procedure requires eggs or milk or anything else I need the same regulation charts on all of this. h. if milk please locate the cow! i . if chickens please locate the chicken and all the infor related to that, j. if butter or margarine is used please submit all the qc on those products. k. almost forgot I need all the info from your oven too. you know the drill And last but not least please fed ex this infor along with the peach cobbler so I can give you a full assesment of the product. I will post this to the histonet only after you get a pin number to see the results. If I have missed anything just include it in the packet and I'm sure I can use it in my evaluation. Thanks again Joe will be looking forward to the package tomorrow. Connie G. > From: jnocito@satx.rr.com > To: histonet@pathology.swmed.edu > Date: Sun, 16 Sep 2007 09:15:35 -0500 > CC: > Subject: [Histonet] not histology related > > calling all you cooks. bakers, and chemistry people, I'm making peach > cobbler. When I'm making pies or some goody that requires pastry, all my recipes have 2 cups flour and 3-4 tablespoons of ice water. My question is: do you use 3-4 TSB of water? This morning, I used almost 1/2 cup. As long as I've been baking, I've never used 3-4 TSB of water. I don't know why this bothers me, but it does. Sometimes my crust is too dry, other times, it's soggy. What is a histotech to do? Thanks y'all > > JTT > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ More photos; more messages; more whatever - Get MORE with Windows Live(tm) Hotmail?. NOW with 5GB storage. http://imagine-windowslive.com/hotmail/?locale=en-us&ocid=TXT_TAGHM_migratio n_HM_mini_5G_0907_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Shape Yahoo! in your own image. Join our Network Research Panel today! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Sep 17 07:58:40 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Sep 17 08:00:15 2007 Subject: [Histonet] Re: negative controls In-Reply-To: <8C9C73CAE3D237F-9AC-55E@FWM-D09.sysops.aol.com> Message-ID: <109116.87489.qm@web61219.mail.yahoo.com> Even so. Remember that with the negative control you are trying to characterize any background or "suspicious" reaction as just background noise. Again, the positive control, no matter how many slides or cases, could be just one because with it you want to demonstrate that the procedure worked. I realize that in cases with many chips or needle prostate biopsies, there are too many slides to deal with. This is decision that is not yours to make. Ask you pathologist or medical director, present him/her with the problem and explain that IF there were to be just 1 negative, any reaction in one section had to be interpreted on both structural and reactivity characteristics. IF s/he accepts that argument, that is his/her decision. Ren? J. godsgalnow@aol.com wrote: But what if all of the blocks are prostate core biopsies? Roxanne -----Original Message----- From: Amos Brooks To: histonet@lists.utsouthwestern.edu Sent: Sat, 15 Sep 2007 11:16 pm Subject: [Histonet] Re: negative controls Hypothetically: Let's say an endometriosis case has several parts as they commonly do. A) Uterus bx (obviously), B) Ovary, C) Lg Colon bx, D) Kidney E) Liver Bx. So an IHC is ordered on the case and randomly part A is chosen as a representative negative control. Have you ever noticed how much endogenous biotin is in kidney & liver? Since there is not a negative control on it there is no real way of knowing that the labeling that one would see there is not real. Honestly having a negative on each block is best, if not for eash test being run (a tall order for sure!). Before polymer detection became popular I once used kidney as a positive control for CD10. It labels the brush boarder of the proximal convuluded tubule really nicely. Problem is that this is also a great place to find endogenous biotin! This really sold me on the use of negative controls. Thanks Mary! Have a nice day, Amos Message: 1 Date: Fri, 14 Sep 2007 15:35:48 -0400 From: godsgalnow@aol.com Subject: [Histonet] negative controls To: histonet@lists.utsouthwestern.edu Message-ID: <8C9C51D962FF31B-284-1FE4@webmail-md17.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" How many non-CAP labs out there are doing negative controls on every block that you do an antibody on? If you have a case that goes A-J and they are all 1 block each and you do an AE1/AE3 on blocks B,C,F,& J, will you do a negative on every block or just one for the entire case? This has been an ongoing debate with us.? We typincally only do 1 negative control for the case and given the above example, it might be on block A.? We ususally cut an extra on every block we cut and when the IHC is requested we just go and pull it and for the negative, we just grab whatever slide is left to run the negative on.? What is everyone else doing?? Roxanne _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ Email and AIM finally together. You've gotta check out free AOL Mail! - http://mail.aol.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Fussy? Opinionated? Impossible to please? Perfect. Join Yahoo!'s user panel and lay it on us. From rjbuesa <@t> yahoo.com Mon Sep 17 08:00:55 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Sep 17 08:01:07 2007 Subject: [Histonet] negative controls In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB734A@EMAIL.archildrens.org> Message-ID: <253414.26929.qm@web61225.mail.yahoo.com> That is exactly the way it should be done! Ren? J. "Horn, Hazel V" wrote: We do a negative control on each block and sometimes we do 2. If several antibodies are ordered on one block and their species are different, (i.e. rabbit, mouse) we run one negative mouse and one negative rabbit. Each block should have its own negative control. Hazel Horn --------------------------------- Don't let your dream ride pass you by. Make it a reality with Yahoo! Autos. From simmonsca <@t> upmc.edu Mon Sep 17 08:11:29 2007 From: simmonsca <@t> upmc.edu (Simmons, Christopher) Date: Mon Sep 17 08:15:55 2007 Subject: [Histonet] I think I will give it an acronym In-Reply-To: References: <517393.84936.qm@web61218.mail.yahoo.com> Message-ID: <88D6F512ADC2CB428B90845D2D1388E303A5BB78@1upmc-msx6.acct.upmchs.net> Leica is actually under a company called Danaher (DHR) they are the ones buying up companies all over. Beware ThermoFisher.... Chris Simmons Lead Technologist UPMC Presbyterian Histology 412.647.7660 desk 13242 pager -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Philip Oshel Sent: Monday, September 17, 2007 8:46 AM To: Histonet@Pathology.swmed.edu Subject: RE: [Histonet] I think I will give it an acronym Maybe we should start a pool on how long before it's all ThermoLeica. Phli If I recall correctly, Leica (once named Ernst Leitz, Wetzlar) bought the microscopes and microtomes sections of Carl Reichert (Austria), Wild-Heerburg (Switzerland), American Optical and Bausch and Lomb (USA). After Vision ByoSystems (Australia) grew enough to buy NovoCastra (UK), it was bought by Leica after a failed hostile takeover by Ventana. Now Leica Microsystems can offer all types of consumables, slides and cassettes printers, sells the Peloris tissue processors and the Bond autostainers, manufactures/sells embedding stations, micrtotomes, microscopes, cryostats all sorts of histology automated instruments and has even developed a type of microscope (I call it "Cyclops" because it does not carry "eyes" = eyepieces and is designed just for digital imaging). It is also manufacturing very cheap (and not very good microscopes) in China (any surprises there?). At this moment in Europe only Carl Zeiss (Germany) and Lomo (Russia still manufacturing variations of 1937 Zeiss microscopes) and some companies in Russia (manufacturing copies of the horizontal OmE Reichert microtomes and copies of the Autotechnicon) can be considered as independent from Leica. Is that Thermo larger than Leica Microsystems? Ren? J. Bernice Frederick wrote: One on many. Leica now has vision biosystems! And they were AO long, long ago! Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Philip Oshel Sent: Friday, September 14, 2007 3:05 PM To: Histonet@Pathology.swmed.edu Subject: RE: [Histonet] I think I will give it an acronym How about the Empire? That's what they've become. Phil >The acronym is still longer than most company names. > >Douglas D. Deltour HT(ASCP) >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonner, >Janet >Sent: Friday, September 14, 2007 2:32 PM >To: Gayle Callis; curtis.king@thermofisher.com; >Histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] I think I will give it an acronym > >Honestly, they need to start over with a new name! > >________________________________ > >From: histonet-bounces@lists.utsouthwestern.edu on behalf of Gayle >Callis >Sent: Fri 9/14/2007 3:07 PM >To: curtis.king@thermofisher.com; Histonet@lists.utsouthwestern.edu >Subject: [Histonet] I think I will give it an acronym > > > >Curtis, > >I think I will now assign an acronym, TFSci or maybe ThermFishSci. Too >long to type out all the time. > > >>Subject: RE: [Histonet] AO 860 sliding microtome and Caroline Bass >>Date: Fri, 14 Sep 2007 14:03:12 -0400 >> >>From: "King, Curtis - RAS" >> >>Just to clear things up The Company Name is ThermoFisher Scientific. >> >>Thermo Shandon is a Brand just like Thermo Richard Allen is now a brand. >> >>Curtis D King, HT (ASCP) >>Research and Development >>ThermoFisher Scientific >>4481 Campus Drive >>Kalamazoo, MI 49008 >>800-522-7270 ext. 672 >>Curtis.King@thermofisher.com >> >> > >Gayle Callis >MT,HT,HTL(ASCP) >Research Histopathology Supervisor >Veterinary Molecular Biology >Montana State University - Bozeman >PO Box 173610 >Bozeman MT 59717-3610 -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Check out the hottest 2008 models today at Yahoo! Autos. -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Mon Sep 17 09:40:39 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Mon Sep 17 09:41:08 2007 Subject: [Histonet] I think I will give it an acronym In-Reply-To: <00fb01c7f925$d22fe990$d00f7ca5@lurie.northwestern.edu> References: <357380.60593.qm@web61223.mail.yahoo.com> <00fb01c7f925$d22fe990$d00f7ca5@lurie.northwestern.edu> Message-ID: How about "HISTOSTUFFRUS" -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: 17 September 2007 13:25 To: 'Rene J Buesa'; 'Gayle Callis'; curtis.king@thermofisher.com; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] I think I will give it an acronym AO/Reichert/Leica/visionbiosystems (which includes Novacastra). I guess we have an Empire and a Kingdom. Where do we fit Dako/Acis? Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Friday, September 14, 2007 3:41 PM To: Bernice Frederick; 'Gayle Callis'; curtis.king@thermofisher.com; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] I think I will give it an acronym Which is the compiting enterprise's acronym? Ren? J. Bernice Frederick wrote: Not to mention UpstateMilliporeLabvision all tied to Fisher etc! Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Friday, September 14, 2007 2:07 PM To: curtis.king@thermofisher.com; Histonet@lists.utsouthwestern.edu Subject: [Histonet] I think I will give it an acronym Curtis, I think I will now assign an acronym, TFSci or maybe ThermFishSci. Too long to type out all the time. >Subject: RE: [Histonet] AO 860 sliding microtome and Caroline Bass >Date: Fri, 14 Sep 2007 14:03:12 -0400 > >From: "King, Curtis - RAS" > >Just to clear things up The Company Name is ThermoFisher Scientific. > >Thermo Shandon is a Brand just like Thermo Richard Allen is now a brand. > >Curtis D King, HT (ASCP) >Research and Development >ThermoFisher Scientific >4481 Campus Drive >Kalamazoo, MI 49008 >800-522-7270 ext. 672 >Curtis.King@thermofisher.com > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____ Catch up on fall's hot new shows on Yahoo! TV. Watch previews, get listings, and more! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From debbie_hogg <@t> hotmail.com Mon Sep 17 09:45:34 2007 From: debbie_hogg <@t> hotmail.com (Debbie Hogg) Date: Mon Sep 17 09:45:51 2007 Subject: [Histonet] Surgical specimens In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F3B22@sjhaexc02.sjha.org> Message-ID: I am hospital based here in Canada and Surgical specimens are delivered to our pathology lab by circulator's. Our assistant does go to the Ambulatory Care Unit to pick up the small specimens. The last hospital that I worked at also had designated times that the surgicals would be delivered to the Path. lab. Technologist's have more than enough work to keep them busy without running to the OR for specimens! Debbie Hogg _________________________________________________________________ Put Your Face In Your Space [1]with Windows Live Spaces! References 1. http://g.msn.com/8HMBENCA/2728??PS=47575 From Ngale <@t> bccancer.bc.ca Mon Sep 17 10:47:33 2007 From: Ngale <@t> bccancer.bc.ca (Gale, Nadia) Date: Mon Sep 17 10:47:50 2007 Subject: [Histonet] Background on FFPE mouse tissue Message-ID: Hello Histonetters, I am relatively new to the mouse world of IHC having come from a clinical background into research and have what might be a simple problem... I am experiencing heavy background with the majority of mouse tissues I am staining. I am using the Ventana Discovery system with their universal DAB kit and "titrate" 100uL of antibody diluent onto my negative control slides. I've tried a biotin blocking kit as well (even though it doesn't look like biotin, thought I'd give it a try) and observed no decrease in the amount of background staining. Any ideas for me? Thanks in advance! Nadia Nadia Gale Lead Histotechnologist Centre for Translational and Applied Genomics (CTAG) 3427-600 West 10th Avenue Vancouver, BC V5Z 4E6 tel 604-877-6000 ext 2426 fax 604-877-6089 ngale@bccancer.bc.ca From PMonfils <@t> Lifespan.org Mon Sep 17 11:31:58 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Mon Sep 17 11:32:10 2007 Subject: [Histonet] Background on FFPE mouse tissue In-Reply-To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E273CE3@LSRIEXCH1.lsmaster.lifespan.org> Are you using mouse monoclonal antibodies? Heavy background staining is a common problem when your antibody was produced in the same species that your tissue sample came from. The anti-mouse secondary antibody can't distinguish between the applied mouse primary and the endogenous immunoglobulins naturally present in the mouse tissue. You can avoid this either by using a mouse-on-mouse kit, available from several vendors, or by using primariy antibodies made in other species. From amosbrooks <@t> gmail.com Mon Sep 17 11:40:24 2007 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Mon Sep 17 11:40:36 2007 Subject: [Histonet] Re: negative controls In-Reply-To: <8C9C73CAE3D237F-9AC-55E@FWM-D09.sysops.aol.com> References: <582736990709152016xf425442ne92ab1589a5aac91@mail.gmail.com> <8C9C73CAE3D237F-9AC-55E@FWM-D09.sysops.aol.com> Message-ID: <582736990709170940y19d73ff4re2eab837bd7a2277@mail.gmail.com> Roxanne, Is there any possibility that the multiple cores are from different parts of the prostate that may have metastasises from other places like kidney & liver? Ultimately you can do this how you like. It is just important to know the limitations of your testing and be aware that this can bite you in the a$$ later on. Just my $0.02 Amos On 9/17/07, godsgalnow@aol.com wrote: > > > But what if all of the blocks are prostate core biopsies? > Roxanne > > > -----Original Message----- > From: Amos Brooks > To: histonet@lists.utsouthwestern.edu > Sent: Sat, 15 Sep 2007 11:16 pm > Subject: [Histonet] Re: negative controls > > Hypothetically: > Let's say an endometriosis case has several parts as they commonly do. > A) Uterus bx (obviously), B) Ovary, C) Lg Colon bx, D) Kidney E) Liver Bx. > So an IHC is ordered on the case and randomly part A is chosen as a > representative negative control. Have you ever noticed how much endogenous > biotin is in kidney & liver? Since there is not a negative control on it > there is no real way of knowing that the labeling that one would see there > is not real. > Honestly having a negative on each block is best, if not for eash test > being run (a tall order for sure!). Before polymer detection became popular > I once used kidney as a positive control for CD10. It labels the brush > boarder of the proximal convuluded tubule really nicely. Problem is that > this is also a great place to find endogenous biotin! This really sold me on > the use of negative controls. Thanks Mary! > > Have a nice day, > Amos > > Message: 1 > Date: Fri, 14 Sep 2007 15:35:48 -0400 > From: godsgalnow@aol.com > Subject: [Histonet] negative controls > To: histonet@lists.utsouthwestern.edu > Message-ID: <8C9C51D962FF31B-284-1FE4@webmail-md17.sysops.aol.com > > > Content-Type: text/plain; charset="us-ascii" > > How many non-CAP labs out there are doing negative controls on every block > that you do an antibody on? > > If you have a case that goes A-J and they are all 1 block each and you do an > AE1/AE3 on blocks B,C,F,& J, will you do a negative on every block or just > one for the entire case? > > This has been an ongoing debate with us.? We typincally only do 1 negative > control for the case and given the above example, it might be on block A.? > We ususally cut an extra on every block we cut and when the IHC is requested > we just go and pull it and for the negative, we just grab whatever slide is > left to run the negative on.? What is everyone else doing?? > > Roxanne > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > Email and AIM finally together. You've gotta check out free AOL Mail > ! > From Marirose.Satterfield <@t> MercyMemorial.org Mon Sep 17 11:37:45 2007 From: Marirose.Satterfield <@t> MercyMemorial.org (Satterfield, Marirose) Date: Mon Sep 17 11:42:22 2007 Subject: [Histonet] Formalin & FNA Message-ID: Actually I have two different questions. #1 Who performs the FNA procedures at your facility? #2 How many people have their microtomy, staining, tissue processing and grossing all in one room? I am doing a little bit of research and any answers will help. Thanks! Mari S. From amosbrooks <@t> gmail.com Mon Sep 17 11:47:35 2007 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Mon Sep 17 11:47:48 2007 Subject: [Histonet] I think I will give it an acronym Message-ID: <582736990709170947p15ceb7fcg1327ed43ae033966@mail.gmail.com> Or better yet : ALLYOURHISTOSTUFFAREBELONGTOUS (let's see how many geeks we have on the histonet) Amos Message: 20 Date: Mon, 17 Sep 2007 15:40:39 +0100 From: "Edwards, R.E." Subject: RE: [Histonet] I think I will give it an acronym To: "Bernice Frederick" , "Rene J Buesa" , "Gayle Callis" , , Message-ID: Content-Type: text/plain; charset="iso-8859-1" How about "HISTOSTUFFRUS" From MadaryJ <@t> MedImmune.com Mon Sep 17 12:09:32 2007 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Mon Sep 17 12:10:02 2007 Subject: [Histonet] mouse IHC background In-Reply-To: Message-ID: <8F3E1865E343C943BB38506D56FF015101013CC0@MD1EV002.medimmune.com> Maybe a mouse adsorbed secondary is all you need. Vector makes them. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Monday, September 17, 2007 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 46, Issue 30 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Surgical specimens (Debbie Hogg) 2. Background on FFPE mouse tissue (Gale, Nadia) 3. RE: Background on FFPE mouse tissue (Monfils, Paul) 4. Re: Re: negative controls (Amos Brooks) 5. Formalin & FNA (Satterfield, Marirose) 6. RE: I think I will give it an acronym (Amos Brooks) ---------------------------------------------------------------------- Message: 1 Date: Mon, 17 Sep 2007 10:45:34 -0400 From: "Debbie Hogg" Subject: RE: [Histonet] Surgical specimens To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; format="flowed" I am hospital based here in Canada and Surgical specimens are delivered to our pathology lab by circulator's. Our assistant does go to the Ambulatory Care Unit to pick up the small specimens. The last hospital that I worked at also had designated times that the surgicals would be delivered to the Path. lab. Technologist's have more than enough work to keep them busy without running to the OR for specimens! Debbie Hogg _________________________________________________________________ Put Your Face In Your Space [1]with Windows Live Spaces! References 1. http://g.msn.com/8HMBENCA/2728??PS=47575 ------------------------------ Message: 2 Date: Mon, 17 Sep 2007 08:47:33 -0700 From: "Gale, Nadia" Subject: [Histonet] Background on FFPE mouse tissue To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hello Histonetters, I am relatively new to the mouse world of IHC having come from a clinical background into research and have what might be a simple problem... I am experiencing heavy background with the majority of mouse tissues I am staining. I am using the Ventana Discovery system with their universal DAB kit and "titrate" 100uL of antibody diluent onto my negative control slides. I've tried a biotin blocking kit as well (even though it doesn't look like biotin, thought I'd give it a try) and observed no decrease in the amount of background staining. Any ideas for me? Thanks in advance! Nadia Nadia Gale Lead Histotechnologist Centre for Translational and Applied Genomics (CTAG) 3427-600 West 10th Avenue Vancouver, BC V5Z 4E6 tel 604-877-6000 ext 2426 fax 604-877-6089 ngale@bccancer.bc.ca ------------------------------ Message: 3 Date: Mon, 17 Sep 2007 12:31:58 -0400 From: "Monfils, Paul" Subject: RE: [Histonet] Background on FFPE mouse tissue To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E273CE3@LSRIEXCH1.lsmaster.lifespan.org> Content-Type: text/plain; charset="iso-8859-1" Are you using mouse monoclonal antibodies? Heavy background staining is a common problem when your antibody was produced in the same species that your tissue sample came from. The anti-mouse secondary antibody can't distinguish between the applied mouse primary and the endogenous immunoglobulins naturally present in the mouse tissue. You can avoid this either by using a mouse-on-mouse kit, available from several vendors, or by using primariy antibodies made in other species. ------------------------------ Message: 4 Date: Mon, 17 Sep 2007 12:40:24 -0400 From: "Amos Brooks" Subject: Re: [Histonet] Re: negative controls To: "godsgalnow@aol.com" Cc: histonet@lists.utsouthwestern.edu Message-ID: <582736990709170940y19d73ff4re2eab837bd7a2277@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Roxanne, Is there any possibility that the multiple cores are from different parts of the prostate that may have metastasises from other places like kidney & liver? Ultimately you can do this how you like. It is just important to know the limitations of your testing and be aware that this can bite you in the a$$ later on. Just my $0.02 Amos On 9/17/07, godsgalnow@aol.com wrote: > > > But what if all of the blocks are prostate core biopsies? > Roxanne > > > -----Original Message----- > From: Amos Brooks > To: histonet@lists.utsouthwestern.edu > Sent: Sat, 15 Sep 2007 11:16 pm > Subject: [Histonet] Re: negative controls > > Hypothetically: > Let's say an endometriosis case has several parts as they commonly do. > A) Uterus bx (obviously), B) Ovary, C) Lg Colon bx, D) Kidney E) Liver Bx. > So an IHC is ordered on the case and randomly part A is chosen as a > representative negative control. Have you ever noticed how much endogenous > biotin is in kidney & liver? Since there is not a negative control on it > there is no real way of knowing that the labeling that one would see there > is not real. > Honestly having a negative on each block is best, if not for eash test > being run (a tall order for sure!). Before polymer detection became popular > I once used kidney as a positive control for CD10. It labels the brush > boarder of the proximal convuluded tubule really nicely. Problem is that > this is also a great place to find endogenous biotin! This really sold me on > the use of negative controls. Thanks Mary! > > Have a nice day, > Amos > > Message: 1 > Date: Fri, 14 Sep 2007 15:35:48 -0400 > From: godsgalnow@aol.com > Subject: [Histonet] negative controls > To: histonet@lists.utsouthwestern.edu > Message-ID: <8C9C51D962FF31B-284-1FE4@webmail-md17.sysops.aol.com > > > Content-Type: text/plain; charset="us-ascii" > > How many non-CAP labs out there are doing negative controls on every block > that you do an antibody on? > > If you have a case that goes A-J and they are all 1 block each and you do an > AE1/AE3 on blocks B,C,F,& J, will you do a negative on every block or just > one for the entire case? > > This has been an ongoing debate with us.? We typincally only do 1 negative > control for the case and given the above example, it might be on block A.? > We ususally cut an extra on every block we cut and when the IHC is requested > we just go and pull it and for the negative, we just grab whatever slide is > left to run the negative on.? What is everyone else doing?? > > Roxanne > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > Email and AIM finally together. You've gotta check out free AOL Mail > ! > ------------------------------ Message: 5 Date: Mon, 17 Sep 2007 12:37:45 -0400 From: "Satterfield, Marirose" Subject: [Histonet] Formalin & FNA To: Message-ID: Content-Type: text/plain; charset="us-ascii" Actually I have two different questions. #1 Who performs the FNA procedures at your facility? #2 How many people have their microtomy, staining, tissue processing and grossing all in one room? I am doing a little bit of research and any answers will help. Thanks! Mari S. ------------------------------ Message: 6 Date: Mon, 17 Sep 2007 12:47:35 -0400 From: "Amos Brooks" Subject: RE: [Histonet] I think I will give it an acronym To: histonet@lists.utsouthwestern.edu Message-ID: <582736990709170947p15ceb7fcg1327ed43ae033966@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Or better yet : ALLYOURHISTOSTUFFAREBELONGTOUS (let's see how many geeks we have on the histonet) Amos Message: 20 Date: Mon, 17 Sep 2007 15:40:39 +0100 From: "Edwards, R.E." Subject: RE: [Histonet] I think I will give it an acronym To: "Bernice Frederick" , "Rene J Buesa" , "Gayle Callis" , , Message-ID: Content-Type: text/plain; charset="iso-8859-1" How about "HISTOSTUFFRUS" ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 46, Issue 30 **************************************** From jm.lapointe <@t> accellab.com Mon Sep 17 12:17:44 2007 From: jm.lapointe <@t> accellab.com (Jean-Martin Lapointe) Date: Mon Sep 17 12:19:53 2007 Subject: [Histonet] re: Background on FFPE mouse tissue Message-ID: As Paul suggested, my first thought is that the background is due to the use of mouse monoclonals. A mouse-on-mouse kit may help, but my limited experience with these has not been very positive; they may be a bit unpredictable, and/or cause a marked decrease in the signal. Much preferable is to use primary antibodies from another species. _________________________________ Jean-Martin Lapointe, DMV, MS, dACVP AccelLAB Inc From awatanabe <@t> tgen.org Mon Sep 17 12:29:00 2007 From: awatanabe <@t> tgen.org (Aprill Watanabe) Date: Mon Sep 17 12:29:18 2007 Subject: [Histonet] Re: Background on FFPE mouse tissues In-Reply-To: <20070917172016.06A424036A@mr1.tgen.org> Message-ID: I try to use a different primary antibody other than mouse and make sure that the secondary is not mouse either. I also use a universal protein block and a rodent block reagent from Biocare that has shown some promise in my lab. I do a lot of work with mouse xenograft tissues so I get high background with most staining. These reagent changes for us have made at least a noticeable difference. Aprill Watanabe, B.S. Research Associate Tissue Microarray Center (TMA) Translational Genomics Research Institute (TGen) 602-343-8822 awatanabe@tgen.org www.tgen.org From jnocito <@t> satx.rr.com Mon Sep 17 12:35:15 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Mon Sep 17 12:35:26 2007 Subject: [Histonet] not histology related References: <010e01c7f928$e065e250$d00f7ca5@lurie.northwestern.edu> Message-ID: <007e01c7f951$1c343840$0202a8c0@yourxhtr8hvc4p> nnnnnnnooooooooooooooooo!!!!!!! ----- Original Message ----- From: "Bernice Frederick" To: "'Rene J Buesa'" ; "'connie grubaugh'" ; "'Joe Nocito'" ; "'histonet'" Sent: Monday, September 17, 2007 7:47 AM Subject: RE: [Histonet] not histology related This sounds like we are still on Friday. Joe, don't forget that if you are using a microwave we need all the specs for that too! Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Sunday, September 16, 2007 11:19 AM To: connie grubaugh; Joe Nocito; histonet Subject: RE: [Histonet] not histology related And the pages numbers of the SOP (signed) where the procedure is described, along with the references used. Ren? J. connie grubaugh wrote: Joe, I need a little more information. 1. please submit the following, a. the temperature chart of the kitchen before and after you do the cooking. along with the calibration info from the precise calibrated thermometers that you are using and the lot number. b. the temperature of the water and the lot number from the local water dept. c. the lot number of where ever the peaches came from (from outside on a tree the lot number from your local nursery or from where ever you got them if fresh) or the lot number of the can and what grocery store you received them from and the manager of the stores mothers maiden name. d. the counter disinfectant's chart and what type of cleanser you are using. e. the lot number and QC chart from the baking pan or whatever you are using there, f. the calibrating information of the cooking utensils that you are using. g. if this procedure requires eggs or milk or anything else I need the same regulation charts on all of this. h. if milk please locate the cow! i . if chickens please locate the chicken and all the infor related to that, j. if butter or margarine is used please submit all the qc on those products. k. almost forgot I need all the info from your oven too. you know the drill And last but not least please fed ex this infor along with the peach cobbler so I can give you a full assesment of the product. I will post this to the histonet only after you get a pin number to see the results. If I have missed anything just include it in the packet and I'm sure I can use it in my evaluation. Thanks again Joe will be looking forward to the package tomorrow. Connie G. > From: jnocito@satx.rr.com > To: histonet@pathology.swmed.edu > Date: Sun, 16 Sep 2007 09:15:35 -0500 > CC: > Subject: [Histonet] not histology related > > calling all you cooks. bakers, and chemistry people, > I'm making peach cobbler. When I'm making pies or some goody that requires pastry, all my recipes have 2 cups flour and 3-4 tablespoons of ice water. My question is: do you use 3-4 TSB of water? This morning, I used almost 1/2 cup. As long as I've been baking, I've never used 3-4 TSB of water. I don't know why this bothers me, but it does. Sometimes my crust is too dry, other times, it's soggy. What is a histotech to do? Thanks y'all > > JTT > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ More photos; more messages; more whatever - Get MORE with Windows LiveT Hotmail?. NOW with 5GB storage. http://imagine-windowslive.com/hotmail/?locale=en-us&ocid=TXT_TAGHM_migratio n_HM_mini_5G_0907_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Shape Yahoo! in your own image. Join our Network Research Panel today! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pkrein <@t> ventanamed.com Mon Sep 17 12:36:32 2007 From: pkrein <@t> ventanamed.com (Peter Krein) Date: Mon Sep 17 12:36:52 2007 Subject: [Histonet] unsubscribe Message-ID: <4F9A1711C1095543B652EC7EEEC157D01B82F6B5@BLUEMAX.VENTANA.VENTANAMED.COM> From jnocito <@t> satx.rr.com Mon Sep 17 12:39:19 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Mon Sep 17 12:39:29 2007 Subject: [Histonet] Thanks Message-ID: <00a801c7f951$ad313960$0202a8c0@yourxhtr8hvc4p> just want to say thanks for all those who responded to my pie crust problems. The cobbler came out ok, but juicy. The crust was tasty and crisp. I'm so confused, but that's my natural state. JTT From gcallis <@t> montana.edu Mon Sep 17 12:54:26 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Sep 17 12:58:37 2007 Subject: [Histonet] re: Background on FFPE mouse tissue In-Reply-To: References: Message-ID: <6.0.0.22.1.20070917114656.01b340c0@gemini.msu.montana.edu> Good point, rat antimouse, hamster antimouse, or rabbit antimouse (if they have a monoclonal for mouse made in rabbit) or rabbit antihuman. Not only secondaries adsorbed to mouse, but also use mouse serum in diluents/normal serum blocking. We prefer F(ab')2 frag of IgG secondaries, adsorbed to mouse in order to eliminate fc receptor binding. Jackson has excellent antibodies for that purpose. Many of the kits for clinical use contain multilinks for mouse, rabbit and goat. If you use a single link polymer kit for rodents, goat, rabbit, you will eliminate biotin and the mouse on mouse problems. Biocare Promark single links work very well since they have an array of single links useful to research. I believer Vision Biosystems also has this available. If it is a mouse on mouse problem and you must use a mouse monoclonal, then kits that biotinylate the primary are superb. DAKO ARK and Biocare have excellent kits for this purpose. A lady gave an excellent poster on this at NSH a few years back, and her results were very convincing for the use of this kind of kit, no secondary antibodies are used at all. At 11:17 AM 9/17/2007, you wrote: >As Paul suggested, my first thought is that the background is due to the >use of mouse monoclonals. A mouse-on-mouse kit may help, but my limited >experience with these has not been very positive; they may be a bit >unpredictable, and/or cause a marked decrease in the signal. Much >preferable is to use primary antibodies from another species. > > > >_________________________________ > >Jean-Martin Lapointe, DMV, MS, dACVP > >AccelLAB Inc > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From Jan.Minshew <@t> leica-microsystems.com Mon Sep 17 13:23:45 2007 From: Jan.Minshew <@t> leica-microsystems.com (Jan.Minshew@leica-microsystems.com) Date: Mon Sep 17 13:24:08 2007 Subject: [Histonet] I think I will give it an acronym In-Reply-To: <517393.84936.qm@web61218.mail.yahoo.com> Message-ID: Hi Ren?, I read your comments below and forwarded them to Doug Giszczynski, Leica's Senior Marketing Manager for clinical microscopy in the US. I thought you might enjoy receiving his comments (see below) so you can share more accurate information with Histonet readers in the future. I must agree that our newest clinical microscope looks different than what has been seen in the past. Your nickname, the "Cyclops", is interesting (I think it looks like a stapler on steroids). Other than it's unique appearance, it was designed to address the (not too) future need for image capture and sharing, and it's doing it very well. Another great thing is that it's ergonomic and that's a VERY important issue for people who sit at microscopes all day. At any rate...read on and learn! Best wishes, Jan ----- Forwarded by Jan Minshew/USDER/West/Leica on 09/17/2007 12:21 PM ----- Doug Giszczynski/USDER /West/Leica To Jan Minshew/USDER/West/Leica@Leica 09/17/2007 11:19 cc AM Subject Re: Fw: [Histonet] I think I will give it an acronym(Document link: Jan Minshew) Hi Jan, Thank you for the opportunity to clarify some of the confusion presented in the opinion below. << Now Leica Microsystems can offer all types of consumables, slides and cassettes printers, sells the Peloris tissue processors and the Bond autostainers, manufactures/sells embedding stations, micrtotomes, microscopes, cryostats all sorts of histology automated instruments and has even developed a type of microscope (I call it "Cyclops" because it does not carry "eyes" = eyepieces and is designed just for digital imaging). It is also manufacturing very cheap (and not very good microscopes) in China (any surprises there?). Ren? J.>> The instrument is actually called the Leica DMD108. It is a digital microscope with on-board computer and ethernet card designed to make it easy to capture and share histopathology images. While Leica has gone to great pains to make this instrument affordable, it is very well constructed, reliable, and easy to use. It was designed and is manufactured (with the exception of the camera chip and electronics), assembled, and 100% quality inspected in Wetzlar Germany. Most of Leica's high performance optics still are. This is the same factory that has been Leitz / Leica's headquarters for almost 100 years. As for the electronics, all microscope manufacturers that I am aware of outsource camera chips and PC boards as we do not require large enough volumes to allow efficient and cost-effective production. Best regards, Doug Douglas Giszczynski Sr. Marketing Manager Leica Microsystems, Biosystems Division Ph 847 317 7207 Fx 847 236 3041 Cell 847 687 1582 Doug.Giszczynski@Leica-Microsystems.com www.Leica-Microsystems.com Jan Minshew/USDER/Wes t/Leica To Doug Giszczynski 09/14/2007 04:52 cc PM Subject Fw: [Histonet] I think I will give it an acronym ??? ----- Forwarded by Jan Minshew/USDER/West/Leica on 09/14/2007 04:52 PM ----- Rene J Buesa To Sent by: Bernice Frederick histonet-bounces@ , lists.utsouthwest 'Philip Oshel' ern.edu , Histonet@Pathology.swmed.edu cc 09/14/2007 04:00 PM Subject RE: [Histonet] I think I will give it an acronym If I recall correctly, Leica (once named Ernst Leitz, Wetzlar) bought the microscopes and microtomes sections of Carl Reichert (Austria), Wild-Heerburg (Switzerland), American Optical and Bausch and Lomb (USA). After Vision ByoSystems (Australia) grew enough to buy NovoCastra (UK), it was bought by Leica after a failed hostile takeover by Ventana. Now Leica Microsystems can offer all types of consumables, slides and cassettes printers, sells the Peloris tissue processors and the Bond autostainers, manufactures/sells embedding stations, micrtotomes, microscopes, cryostats all sorts of histology automated instruments and has even developed a type of microscope (I call it "Cyclops" because it does not carry "eyes" = eyepieces and is designed just for digital imaging). It is also manufacturing very cheap (and not very good microscopes) in China (any surprises there?). At this moment in Europe only Carl Zeiss (Germany) and Lomo (Russia still manufacturing variations of 1937 Zeiss microscopes) and some companies in Russia (manufacturing copies of the horizontal OmE Reichert microtomes and copies of the Autotechnicon) can be considered as independent from Leica. Is that Thermo larger than Leica Microsystems? Ren? J. Bernice Frederick wrote: One on many. Leica now has vision biosystems! And they were AO long, long ago! Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Philip Oshel Sent: Friday, September 14, 2007 3:05 PM To: Histonet@Pathology.swmed.edu Subject: RE: [Histonet] I think I will give it an acronym How about the Empire? That's what they've become. Phil >The acronym is still longer than most company names. > >Douglas D. Deltour HT(ASCP) >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonner, >Janet >Sent: Friday, September 14, 2007 2:32 PM >To: Gayle Callis; curtis.king@thermofisher.com; >Histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] I think I will give it an acronym > >Honestly, they need to start over with a new name! > >________________________________ > >From: histonet-bounces@lists.utsouthwestern.edu on behalf of Gayle Callis >Sent: Fri 9/14/2007 3:07 PM >To: curtis.king@thermofisher.com; Histonet@lists.utsouthwestern.edu >Subject: [Histonet] I think I will give it an acronym > > > >Curtis, > >I think I will now assign an acronym, TFSci or maybe ThermFishSci. Too >long to type out all the time. > > >>Subject: RE: [Histonet] AO 860 sliding microtome and Caroline Bass >>Date: Fri, 14 Sep 2007 14:03:12 -0400 >> >>From: "King, Curtis - RAS" >> >>Just to clear things up The Company Name is ThermoFisher Scientific. >> >>Thermo Shandon is a Brand just like Thermo Richard Allen is now a brand. >> >>Curtis D King, HT (ASCP) >>Research and Development >>ThermoFisher Scientific >>4481 Campus Drive >>Kalamazoo, MI 49008 >>800-522-7270 ext. 672 >>Curtis.King@thermofisher.com >> >> > >Gayle Callis >MT,HT,HTL(ASCP) >Research Histopathology Supervisor >Veterinary Molecular Biology >Montana State University - Bozeman >PO Box 173610 >Bozeman MT 59717-3610 -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Check out the hottest 2008 models today at Yahoo! Autos. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Rene J Buesa To Sent by: Bernice Frederick histonet-bounces@ , lists.utsouthwest 'Philip Oshel' ern.edu , Histonet@Pathology.swmed.edu cc 09/14/2007 04:00 PM Subject RE: [Histonet] I think I will give it an acronym If I recall correctly, Leica (once named Ernst Leitz, Wetzlar) bought the microscopes and microtomes sections of Carl Reichert (Austria), Wild-Heerburg (Switzerland), American Optical and Bausch and Lomb (USA). After Vision ByoSystems (Australia) grew enough to buy NovoCastra (UK), it was bought by Leica after a failed hostile takeover by Ventana. Now Leica Microsystems can offer all types of consumables, slides and cassettes printers, sells the Peloris tissue processors and the Bond autostainers, manufactures/sells embedding stations, micrtotomes, microscopes, cryostats all sorts of histology automated instruments and has even developed a type of microscope (I call it "Cyclops" because it does not carry "eyes" = eyepieces and is designed just for digital imaging). It is also manufacturing very cheap (and not very good microscopes) in China (any surprises there?). At this moment in Europe only Carl Zeiss (Germany) and Lomo (Russia still manufacturing variations of 1937 Zeiss microscopes) and some companies in Russia (manufacturing copies of the horizontal OmE Reichert microtomes and copies of the Autotechnicon) can be considered as independent from Leica. Is that Thermo larger than Leica Microsystems? Ren? J. Bernice Frederick wrote: One on many. Leica now has vision biosystems! And they were AO long, long ago! Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Philip Oshel Sent: Friday, September 14, 2007 3:05 PM To: Histonet@Pathology.swmed.edu Subject: RE: [Histonet] I think I will give it an acronym How about the Empire? That's what they've become. Phil >The acronym is still longer than most company names. > >Douglas D. Deltour HT(ASCP) >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonner, >Janet >Sent: Friday, September 14, 2007 2:32 PM >To: Gayle Callis; curtis.king@thermofisher.com; >Histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] I think I will give it an acronym > >Honestly, they need to start over with a new name! > >________________________________ > >From: histonet-bounces@lists.utsouthwestern.edu on behalf of Gayle Callis >Sent: Fri 9/14/2007 3:07 PM >To: curtis.king@thermofisher.com; Histonet@lists.utsouthwestern.edu >Subject: [Histonet] I think I will give it an acronym > > > >Curtis, > >I think I will now assign an acronym, TFSci or maybe ThermFishSci. Too >long to type out all the time. > > >>Subject: RE: [Histonet] AO 860 sliding microtome and Caroline Bass >>Date: Fri, 14 Sep 2007 14:03:12 -0400 >> >>From: "King, Curtis - RAS" >> >>Just to clear things up The Company Name is ThermoFisher Scientific. >> >>Thermo Shandon is a Brand just like Thermo Richard Allen is now a brand. >> >>Curtis D King, HT (ASCP) >>Research and Development >>ThermoFisher Scientific >>4481 Campus Drive >>Kalamazoo, MI 49008 >>800-522-7270 ext. 672 >>Curtis.King@thermofisher.com >> >> > >Gayle Callis >MT,HT,HTL(ASCP) >Research Histopathology Supervisor >Veterinary Molecular Biology >Montana State University - Bozeman >PO Box 173610 >Bozeman MT 59717-3610 -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Check out the hottest 2008 models today at Yahoo! Autos. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From sheila_adey <@t> hotmail.com Mon Sep 17 13:45:51 2007 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Mon Sep 17 13:46:05 2007 Subject: [Histonet] Formalin & FNA In-Reply-To: References: Message-ID: The histotech prepares the smears for the pathologist on an FNA. Our Microtomy is in a separate room.Sheila Adey HT MLTPort Huron HospitalMichigan> Date: Mon, 17 Sep 2007 12:37:45 -0400> From: Marirose.Satterfield@MercyMemorial.org> To: histonet@lists.utsouthwestern.edu> Subject: [Histonet] Formalin & FNA> > Actually I have two different questions. > > > > #1 Who performs the FNA procedures at your facility?> > > > #2 How many people have their microtomy, staining, tissue processing and> grossing all in one room?> > > > I am doing a little bit of research and any answers will help.> > Thanks!> > > > Mari S.> > > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Discover the new Windows Vista http://search.msn.com/results.aspx?q=windows+vista&mkt=en-US&form=QBRE From lpjones <@t> srhs-pa.org Mon Sep 17 13:45:52 2007 From: lpjones <@t> srhs-pa.org (Jones, Laura) Date: Mon Sep 17 13:46:10 2007 Subject: [Histonet] Workload/Billable Tests and Staffing Message-ID: <8E7AD740937B954F947F0DB4467EFEE056E9DA@mail.srhs-pa.org> Greetings fellow Histotechs. I would appreciate it if anyone could help me or share their own method of reporting workload to the "powers that be"; who in turn use that information to determine staffing in the Histology Lab. In my situation, staffing is being determined based soley on billable tests, which as we all know is not a true indication of the work being done. We are a small community hospital and essentially do it all. We run and get the specimens, accession them, set them up with cassettes, gross and dictate things that do not need to be cut, prepare slides for the next day,cut and run immunos, etc. In short, we are extremely busy, but this is not reflected in "billable tests". Could you please share with me any ideas or formulas you have to more accurately report what you are doing? Or, if you are willing to share your number of billable tests and your number of techs, perhaps I could compile this information and gain some kind of persepctive. I was only recently given this responsibility, and apologize for my naivite, inexperience and ignorance! I'd greatly appreciate your advice and knowledge. Thank you all in advance! Laura From tkngflght <@t> yahoo.com Mon Sep 17 13:57:55 2007 From: tkngflght <@t> yahoo.com (Cheryl R. Kerry) Date: Mon Sep 17 13:57:50 2007 Subject: [Histonet] Workload/Billable Tests and Staffing In-Reply-To: <8E7AD740937B954F947F0DB4467EFEE056E9DA@mail.srhs-pa.org> Message-ID: <00ff01c7f95c$a90ac030$6701a8c0@CHERYLSLAPTOP> Does anyone have the old CAP Accounting method in a procedure somewhere? This is a great place for Laura to start for representing ALL the work done in a lab, not just the billables. Cheryl Cheryl R. Kerry, HT(ASCP) Full Staff Inc. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jones, Laura Sent: Monday, September 17, 2007 1:46 PM To: Histonet (E-mail) Subject: [Histonet] Workload/Billable Tests and Staffing Greetings fellow Histotechs. I would appreciate it if anyone could help me or share their own method of reporting workload to the "powers that be"; who in turn use that information to determine staffing in the Histology Lab. In my situation, staffing is being determined based soley on billable tests, which as we all know is not a true indication of the work being done. We are a small community hospital and essentially do it all. We run and get the specimens, accession them, set them up with cassettes, gross and dictate things that do not need to be cut, prepare slides for the next day,cut and run immunos, etc. In short, we are extremely busy, but this is not reflected in "billable tests". Could you please share with me any ideas or formulas you have to more accurately report what you are doing? Or, if you are willing to share your number of billable tests and your number of techs, perhaps I could compile this information and gain some kind of persepctive. I was only recently given this responsibility, and apologize for my naivite, inexperience and ignorance! I'd greatly appreciate your advice and knowledge. Thank you all in advance! Laura _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Mon Sep 17 14:58:17 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Mon Sep 17 13:58:53 2007 Subject: {SPAM?} RE: [Histonet] I think I will give it an acronym In-Reply-To: <582736990709170947p15ceb7fcg1327ed43ae033966@mail.gmail.com> Message-ID: I just called Erie Scientific about some slides and guess what?? They answered the phone ThermoFisher........ The list keeps on growing. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Monday, September 17, 2007 11:48 AM To: histonet@lists.utsouthwestern.edu Subject: {SPAM?} RE: [Histonet] I think I will give it an acronym Or better yet : ALLYOURHISTOSTUFFAREBELONGTOUS (let's see how many geeks we have on the histonet) Amos Message: 20 Date: Mon, 17 Sep 2007 15:40:39 +0100 From: "Edwards, R.E." Subject: RE: [Histonet] I think I will give it an acronym To: "Bernice Frederick" , "Rene J Buesa" , "Gayle Callis" , , Message-ID: Content-Type: text/plain; charset="iso-8859-1" How about "HISTOSTUFFRUS" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Sep 17 14:12:18 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Sep 17 14:12:31 2007 Subject: [Histonet] I think I will give it an acronym In-Reply-To: Message-ID: <964223.21351.qm@web61211.mail.yahoo.com> Hi Jan: Thank you for the information even when I can "feel" some (unnecessary) animosity behid it because you cannot find anyone more "faithful" to the Ernst Leitz brand than me. I own a Ortholux and a Metalux, as well as 2 Orthomat automated cameras, scores of lenses and even 2 vintage Leicas with an almost unimaginable array of accessories so, in my case, when talking about Leitz (I still don't like the "Leica" name for the microscopes), you are preaching to the choir. I "baptized" your imaging microscope as the "Cyclops" because that is what it looks like to me, and I am sure that the optics, if Wetzlar manufactured, have to be superb and durable, as the set of apochromatic objectives I have in my microscopes. So, yes, live and learn, that has been always my moto. Thank you for the information. Ren? J. ____ --------------------------------- Pinpoint customers who are looking for what you sell. From rjbuesa <@t> yahoo.com Mon Sep 17 14:14:34 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Sep 17 14:14:48 2007 Subject: [Histonet] Workload/Billable Tests and Staffing In-Reply-To: <8E7AD740937B954F947F0DB4467EFEE056E9DA@mail.srhs-pa.org> Message-ID: <359700.23701.qm@web61219.mail.yahoo.com> Laura; Under separate cover I am sending you an article of mine on staffing for histology that may help you with your problem. Ren? J. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Pinpoint customers who are looking for what you sell. From Jan.Minshew <@t> leica-microsystems.com Mon Sep 17 14:24:17 2007 From: Jan.Minshew <@t> leica-microsystems.com (Jan.Minshew@leica-microsystems.com) Date: Mon Sep 17 14:24:33 2007 Subject: [Histonet] I think I will give it an acronym In-Reply-To: <964223.21351.qm@web61211.mail.yahoo.com> Message-ID: Hi Ren?, There was no animosity meant. I was in a bit of a rush when I wrote that, so I'm sorry if it came across as such. I really did find the "Cyclops" name interesting and it was another customer who pointed out to us that it looked like a giant stapler. No matter what it looks like...we're proud of it anyway (spoken like a true mother and grandmother). Best wishes to everyone, Jan Rene J Buesa To Jan.Minshew@leica-microsystems.com 09/17/2007 02:12 cc PM Histonet@Pathology.swmed.edu, histonet-bounces@lists.utsouthweste rn.edu, DGiszczynski@leica-microsystems.com Subject RE: [Histonet] I think I will give it an acronym Hi Jan: Thank you for the information even when I can "feel" some (unnecessary) animosity behid it because you cannot find anyone more "faithful" to the Ernst Leitz brand than me. I own a Ortholux and a Metalux, as well as 2 Orthomat automated cameras, scores of lenses and even 2 vintage Leicas with an almost unimaginable array of accessories so, in my case, when talking about Leitz (I still don't like the "Leica" name for the microscopes), you are preaching to the choir. I "baptized" your imaging microscope as the "Cyclops" because that is what it looks like to me, and I am sure that the optics, if Wetzlar manufactured, have to be superb and durable, as the set of apochromatic objectives I have in my microscopes. So, yes, live and learn, that has been always my moto. Thank you for the information. Ren? J. ____ Pinpoint customers who are looking for what you sell. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From rjbuesa <@t> yahoo.com Mon Sep 17 14:29:08 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Sep 17 14:29:18 2007 Subject: [Histonet] I think I will give it an acronym In-Reply-To: Message-ID: <175528.83270.qm@web61216.mail.yahoo.com> Hi Jan: Understood! Regarding the "Cyclops" it is either a "cyclops" (only one digital eye) or the head of a large "fire ant soldier", in any event, is one of those "faces" only a mother can love, although I am sure that it works as it should (being a Leitz!). Ren? J. Jan.Minshew@leica-microsystems.com wrote: Hi Ren?, There was no animosity meant. I was in a bit of a rush when I wrote that, so I'm sorry if it came across as such. I really did find the "Cyclops" name interesting and it was another customer who pointed out to us that it looked like a giant stapler. No matter what it looks like...we're proud of it anyway (spoken like a true mother and grandmother). Best wishes to everyone, Jan Rene J Buesa m> To Jan.Minshew@leica-microsystems.com 09/17/2007 02:12 cc PM Histonet@Pathology.swmed.edu, histonet-bounces@lists.utsouthweste rn.edu, DGiszczynski@leica-microsystems.com Subject RE: [Histonet] I think I will give it an acronym Hi Jan: Thank you for the information even when I can "feel" some (unnecessary) animosity behid it because you cannot find anyone more "faithful" to the Ernst Leitz brand than me. I own a Ortholux and a Metalux, as well as 2 Orthomat automated cameras, scores of lenses and even 2 vintage Leicas with an almost unimaginable array of accessories so, in my case, when talking about Leitz (I still don't like the "Leica" name for the microscopes), you are preaching to the choir. I "baptized" your imaging microscope as the "Cyclops" because that is what it looks like to me, and I am sure that the optics, if Wetzlar manufactured, have to be superb and durable, as the set of apochromatic objectives I have in my microscopes. So, yes, live and learn, that has been always my moto. Thank you for the information. Ren? J. ____ Pinpoint customers who are looking for what you sell. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ --------------------------------- Yahoo! oneSearch: Finally, mobile search that gives answers, not web links. From alaskagirl1950 <@t> yahoo.com Mon Sep 17 14:33:43 2007 From: alaskagirl1950 <@t> yahoo.com (Patricia Adams) Date: Mon Sep 17 14:33:56 2007 Subject: [Histonet] Thanks In-Reply-To: <00a801c7f951$ad313960$0202a8c0@yourxhtr8hvc4p> Message-ID: <403106.58171.qm@web52506.mail.re2.yahoo.com> Now I am confused.....whats wrong with juicy, tasty and crisp? I thought that was what one reached for???! --- Joe Nocito wrote: > just want to say thanks for all those who > responded to my pie crust problems. The cobbler > came out ok, but juicy. The crust was tasty and > crisp. I'm so confused, but that's my natural > state. > > JTT > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D ____________________________________________________________________________________ Moody friends. Drama queens. Your life? Nope! - their life, your story. Play Sims Stories at Yahoo! Games. http://sims.yahoo.com/ From Ngale <@t> bccancer.bc.ca Mon Sep 17 15:42:32 2007 From: Ngale <@t> bccancer.bc.ca (Gale, Nadia) Date: Mon Sep 17 15:42:47 2007 Subject: [Histonet] Mouse background Message-ID: Thank you to all of you who provided me with insights as to why I might be experiencing so much background with my mouse IHC. I believe I have the answer I was looking for and will let you know if I'm right after a run tomorrow. -Nadia Nadia Gale Lead Histotechnologist Centre for Translational and Applied Genomics (CTAG) 3427-600 West 10th Avenue Vancouver, BC V5Z 4E6 tel 604-877-6000 ext 2426 fax 604-877-6089 ngale@bccancer.bc.ca From agustinvictor <@t> gmail.com Mon Sep 17 19:58:48 2007 From: agustinvictor <@t> gmail.com (Agustin Chertcoff) Date: Mon Sep 17 19:58:57 2007 Subject: [Histonet] SAHNET Message-ID: <002701c7f98f$14f57a60$590215ac@Pentium4> Hi Histonetters! The SAHNET is a listserver (in Spanish Language) for the Histology profession that is managed as a service to the field of Histology by Ht Agustin Victor Chertcoff & Ht Norma Pozzo. SAHNET current has more than 245 members in the world specially Argentina,Peru,Mexico,Costa Rica,Uruguay,Chile,Colombia,Spain. go to http://www.elistas.net/grupo/sahnet visit to www.ht.org.ar Argentine Society of Histotechnology SAH Thanks to all! Agustin Consultas recomendadas Negocios | Presupuestos de seguros | Salud Dental | Seguros de coches -------------------------------------------------------------------------------- -------------------------------------------------------------------------------- Crea tu espacio en el calendario universal de la red Imagina un calendario universal donde cada persona elige una fecha especial (cumplea?os, boda, etc) y crea una pagina en esa fecha, para siempre. www.nuestrosdias.com --------------------------------------------------------------------- Visita la nueva Pagina Web de la SAH http://ht.org.ar Tu direcci?n de suscripci?n a este bolet?n es ribonukle@yahoo.com.ar Para darte de baja, env?a un mensaje a sahnet-baja@eListas.net Para obtener ayuda, visita http://www.eListas.net/lista/sahnet -------------------------------------------------------------------------------- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.5.487 / Virus Database: 269.13.21/1012 - Release Date: 16/9/2007 06:32 p.m. From tahseen <@t> brain.net.pk Mon Sep 17 20:41:18 2007 From: tahseen <@t> brain.net.pk (Tahseen) Date: Mon Sep 17 20:34:58 2007 Subject: [Histonet] Workload/Billable Tests and Staffing References: <8E7AD740937B954F947F0DB4467EFEE056E9DA@mail.srhs-pa.org> Message-ID: <004201c7f995$0293c330$310b80cb@PDualCore> Dear Laura See attachment on staffing for histology that may help you with your problem. Muhammad Tahseen. ----- Original Message ----- From: "Jones, Laura" To: "Histonet (E-mail)" Sent: Monday, September 17, 2007 11:45 PM Subject: [Histonet] Workload/Billable Tests and Staffing > Greetings fellow Histotechs. I would appreciate it if anyone could help > me > or share their own method of reporting workload to the "powers that be"; > who > in turn use that information to determine staffing in the Histology Lab. > > In my situation, staffing is being determined based soley on billable > tests, > which as we all know is not a true indication of the work being done. We > are a small community hospital and essentially do it all. We run and get > the specimens, accession them, set them up with cassettes, gross and > dictate > things that do not need to be cut, prepare slides for the next day,cut and > run immunos, etc. In short, we are extremely busy, but this is not > reflected in "billable tests". > > Could you please share with me any ideas or formulas you have to more > accurately report what you are doing? Or, if you are willing to share > your > number of billable tests and your number of techs, perhaps I could compile > this information and gain some kind of persepctive. I was only recently > given this responsibility, and apologize for my naivite, inexperience and > ignorance! I'd greatly appreciate your advice and knowledge. > > Thank you all in advance! Laura > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From nfournier <@t> sasktel.net Mon Sep 17 22:34:03 2007 From: nfournier <@t> sasktel.net (Neil Fournier) Date: Mon Sep 17 22:34:08 2007 Subject: [Histonet] question about H2O2 use during immunohistochemical protocols Message-ID: <001d01c7f9a4$c312cb10$d5c12fcf@NEIL> Hi everyone, We work with 40 to 50 micron thick fixed (w/ 4% paraformaldehyde) rat brain sections that had been sectioned on a vibrating microtome. The sections are stored for varying lengths of time in a cryoprotectant solution (consisting of sucrose/PVP-40/ethylene glycol in 0.1 M PBS. Watson et al., 1986. Peptides) at -20 degree C. After a thorough washing of the tissue sections several times in PBS rinses, the sections are incubated in 0.3% H2O2/PBS solution (diluted from 30% stock) for 30 to 60 min (depending on protocol). We use Netwells to transfer tissue sections from wash to wash etc. I have noticed that often excessive bubbles form in the culture wells that contain the tissue sections when incubated in the peroxide mixture. At first we thought our stock of previously aliquot 30% H2O2 might have went bad, so I purchased new chemicals and we still observe this bubbling problem. We do not know how this might impact staining quality or if these bubbles might even cause some degree of microscopic damage to the section, but we have noticed lately that some sections show incomplete penetration of the antibody during staining (i.e., staining appears darker for one part of the tissue and lighter for other parts). I do not remember encountering this before. I do not believe it is related to the cryoprotectant b/c this solution is commonly used in several laboratories and I often do around an 1 hr of 5 to 10 min washes before beginning the protocol. I was wondering if anyone here has encountered similar situations when staining tissue and if they know whether this is a problem to staining and what the exact cause might be. Thanking everyone in advance, Neil From Tbarnhart <@t> primecare.org Tue Sep 18 07:14:15 2007 From: Tbarnhart <@t> primecare.org (Barnhart, Tammy) Date: Tue Sep 18 07:13:14 2007 Subject: [Histonet] Workload/Billable Tests and Staffing Message-ID: <4F0B7161A6CD524FAD8017D52E1553407AE550@exchangent> Laura, As we all know billed tests are a terrible way to calculate workload in the histology lab. I have found that using our CLIA numbers (blocks + special stains + immunos + ISH)per hour worked to be a much better indicator of how busy we are. If you have a CLIA certificate, then someone has been submitting these numbers. CLIA uses them to determine how "big" your lab is and thus what your fees will be. Using a standard that is used by a government agency to determine workload is an easier sell to the "bean counters" than some formula you come up with independently. Granted you are not capturing recuts and the like, but if you trend these numbers for your lab, you should be able to track your productivity. Remember, every lab is different so what is happening in your lab is what is important. If your staff is not always getting lunch or breaks or your department has a lot of overtime, then whatever number you are running at now is too high. We had a nationally recognized laboratory productivity assessment company do their comparisons at our lab and they used CLIA numbers for the histology portion. By the way, our CLIA#/worked hour runs around 4.45. Tammy Barnhart, BS, HTL(ASCP) Anatomic Pathology Manager St. Alexius Medical Center Bismarck, ND -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jones, Laura Sent: Monday, September 17, 2007 1:46 PM To: Histonet (E-mail) Subject: [Histonet] Workload/Billable Tests and Staffing Greetings fellow Histotechs. I would appreciate it if anyone could help me or share their own method of reporting workload to the "powers that be"; who in turn use that information to determine staffing in the Histology Lab. In my situation, staffing is being determined based soley on billable tests, which as we all know is not a true indication of the work being done. We are a small community hospital and essentially do it all. We run and get the specimens, accession them, set them up with cassettes, gross and dictate things that do not need to be cut, prepare slides for the next day,cut and run immunos, etc. In short, we are extremely busy, but this is not reflected in "billable tests". Could you please share with me any ideas or formulas you have to more accurately report what you are doing? Or, if you are willing to share your number of billable tests and your number of techs, perhaps I could compile this information and gain some kind of persepctive. I was only recently given this responsibility, and apologize for my naivite, inexperience and ignorance! I'd greatly appreciate your advice and knowledge. Thank you all in advance! Laura _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Sep 18 07:26:25 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Sep 18 07:26:43 2007 Subject: [Histonet] SAFETY IN THE HISTOLOGY LABORATORY Message-ID: <984962.23867.qm@web61215.mail.yahoo.com> Fellow Histonetters: Back in March 2007 ninety three of you answered a safety questionnaire; I sent a summary to each participant, but now the results have been published as an article in Annals of Diagnostic Pathology 11(5):334-339 Those of you interested in the final article can request it and I will send it to you. Thanks for participating in the original survey! Ren? J. --------------------------------- Got a little couch potato? Check out fun summer activities for kids. From talulahgosh <@t> gmail.com Tue Sep 18 08:34:50 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Sep 18 08:35:04 2007 Subject: [Histonet] I think I will give it an acronym In-Reply-To: <582736990709170947p15ceb7fcg1327ed43ae033966@mail.gmail.com> References: <582736990709170947p15ceb7fcg1327ed43ae033966@mail.gmail.com> Message-ID: I'm all about ThermoFisher, since Fisher was started in Pittsburgh. go steelers! (I've been assimilated into the city, not being a true Pittsburgher.) I'll take your allyourbase reference and up it one chocolate rain: chocolate rain thermofisher has no sense of shame Emily -- Remember our war hysteria, when we called sauerkraut 'Liberty cabbage' and somebody actually proposed calling German measles, 'Liberty measles?'...Remember when the hick legislators in certain states, in obedience to William Jennings Bryan, who learned his biology from his pious old grandma, set up shop as scientific experts and made the whole world laugh itself sick by forbidding the teaching of evolution? --Sinclair Lewis, It Can't Happen Here, 1935 From doug <@t> ppspath.com Tue Sep 18 09:51:26 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue Sep 18 08:52:02 2007 Subject: {SPAM?} Re: [Histonet] I think I will give it an acronym In-Reply-To: Message-ID: Did you have to mention football? Do you know how much I dislike Steelers fans? Almost as much as Patriot fans. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, September 18, 2007 8:35 AM To: histonet@lists.utsouthwestern.edu Subject: {SPAM?} Re: [Histonet] I think I will give it an acronym I'm all about ThermoFisher, since Fisher was started in Pittsburgh. go steelers! (I've been assimilated into the city, not being a true Pittsburgher.) I'll take your allyourbase reference and up it one chocolate rain: chocolate rain thermofisher has no sense of shame Emily -- Remember our war hysteria, when we called sauerkraut 'Liberty cabbage' and somebody actually proposed calling German measles, 'Liberty measles?'...Remember when the hick legislators in certain states, in obedience to William Jennings Bryan, who learned his biology from his pious old grandma, set up shop as scientific experts and made the whole world laugh itself sick by forbidding the teaching of evolution? --Sinclair Lewis, It Can't Happen Here, 1935 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Tue Sep 18 09:15:36 2007 From: tkngflght <@t> yahoo.com (Cheryl R. Kerry) Date: Tue Sep 18 09:15:35 2007 Subject: {SPAM?} Re: [Histonet] I think I will give it an acronym Message-ID: <003b01c7f9fe$62c84550$6701a8c0@CHERYLSLAPTOP> Oh Doug--you and I can no longer be friends!!! Born and raised a Steeler fan--in fact, if you don't say it 'STILLERS' you ain't a real fan!! (Go Emily!!) Don't even get me started on the Pirates and the Pens!! Cheryl Cheryl R. Kerry, HT(ASCP) Full Staff Inc. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, September 18, 2007 9:51 AM To: 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym Did you have to mention football? Do you know how much I dislike Steelers fans? Almost as much as Patriot fans. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, September 18, 2007 8:35 AM To: histonet@lists.utsouthwestern.edu Subject: {SPAM?} Re: [Histonet] I think I will give it an acronym I'm all about ThermoFisher, since Fisher was started in Pittsburgh. go steelers! (I've been assimilated into the city, not being a true Pittsburgher.) I'll take your allyourbase reference and up it one chocolate rain: chocolate rain thermofisher has no sense of shame Emily -- Remember our war hysteria, when we called sauerkraut 'Liberty cabbage' and somebody actually proposed calling German measles, 'Liberty measles?'...Remember when the hick legislators in certain states, in obedience to William Jennings Bryan, who learned his biology from his pious old grandma, set up shop as scientific experts and made the whole world laugh itself sick by forbidding the teaching of evolution? --Sinclair Lewis, It Can't Happen Here, 1935 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lynne.Bell <@t> hitchcock.org Tue Sep 18 09:32:35 2007 From: Lynne.Bell <@t> hitchcock.org (Bell, Lynne) Date: Tue Sep 18 09:32:45 2007 Subject: {SPAM?} Re: [Histonet] I think I will give it an acronym In-Reply-To: <200709181401.l8IE1NQH010340@mailhub2.hitchcock.org> Message-ID: Hey, Hey now - I'm a Pats fan................(and a long suffering Sox fan, don't get me started)!! Lynne From TownsendD <@t> childrensdayton.org Tue Sep 18 09:35:16 2007 From: TownsendD <@t> childrensdayton.org (Dolores Townsend) Date: Tue Sep 18 09:35:56 2007 Subject: {SPAM?} Re: [Histonet] I think I will give it an acronym Message-ID: Douglas, Douglas, Douglas Are you out of your mind? Is it that cold in SC? Did you feel a need to be flamed and burned to a crisp? I am not a big fan of football myself, being a woman and European to boot, but I wouldn't dream to go there... Dolores From slappycraw <@t> yahoo.com Tue Sep 18 09:54:40 2007 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Tue Sep 18 09:54:52 2007 Subject: {SPAM?} Re: [Histonet] I think I will give it an acronym Message-ID: <304140.51720.qm@web53610.mail.re2.yahoo.com> The best fans, the only true fans, and America's team, The Green Bay Packers! Need I say more. Douglas D Deltour wrote: Did you have to mention football? Do you know how much I dislike Steelers fans? Almost as much as Patriot fans. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, September 18, 2007 8:35 AM To: histonet@lists.utsouthwestern.edu Subject: {SPAM?} Re: [Histonet] I think I will give it an acronym I'm all about ThermoFisher, since Fisher was started in Pittsburgh. go steelers! (I've been assimilated into the city, not being a true Pittsburgher.) I'll take your allyourbase reference and up it one chocolate rain: chocolate rain thermofisher has no sense of shame Emily -- Remember our war hysteria, when we called sauerkraut 'Liberty cabbage' and somebody actually proposed calling German measles, 'Liberty measles?'...Remember when the hick legislators in certain states, in obedience to William Jennings Bryan, who learned his biology from his pious old grandma, set up shop as scientific experts and made the whole world laugh itself sick by forbidding the teaching of evolution? --Sinclair Lewis, It Can't Happen Here, 1935 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Building a website is a piece of cake. Yahoo! Small Business gives you all the tools to get online. From b-frederick <@t> northwestern.edu Tue Sep 18 09:56:16 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Tue Sep 18 09:56:25 2007 Subject: {SPAM?} Re: [Histonet] I think I will give it an acronym In-Reply-To: Message-ID: <001301c7fa04$13b59930$d00f7ca5@lurie.northwestern.edu> Personally, I am a NASCAR fan. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bell, Lynne Sent: Tuesday, September 18, 2007 9:33 AM To: Douglas D Deltour; Emily Sours; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym Hey, Hey now - I'm a Pats fan................(and a long suffering Sox fan, don't get me started)!! Lynne _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Tue Sep 18 09:57:17 2007 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Sep 18 09:57:32 2007 Subject: [Histonet] Recyclers Message-ID: I'd like some feedback from anyone who has had experience with solvent recyclers. I have literature on the CBG and B/R. What other ones are out there? Is there one company that is efficient at recycling alcohol, xylene and formalin? Thanks in advance for your comments, Toni Rathborne CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. From JWEEMS <@t> sjha.org Tue Sep 18 10:02:01 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Tue Sep 18 10:02:14 2007 Subject: [Histonet] RANTES Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F3BBD@sjhaexc02.sjha.org> Is anyone doing IHC? Would you do for us - stain only? Thanks, Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From hymclab <@t> hyhc.com Tue Sep 18 10:05:32 2007 From: hymclab <@t> hyhc.com (hymclab) Date: Tue Sep 18 10:03:26 2007 Subject: [Possible Spam] RE: {SPAM?} Re: [Histonet] I think I will giv e it an acronym Message-ID: Go Pack!! And guess who's playing in Denver on October 29th on Monday night football while we are there? Can't wait!! Dawn -----Original Message----- From: Larry Woody [mailto:slappycraw@yahoo.com] Sent: Tuesday, September 18, 2007 9:55 AM To: Douglas D Deltour; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: [Possible Spam] RE: {SPAM?} Re: [Histonet] I think I will give it an acronym The best fans, the only true fans, and America's team, The Green Bay Packers! Need I say more. Douglas D Deltour wrote: Did you have to mention football? Do you know how much I dislike Steelers fans? Almost as much as Patriot fans. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, September 18, 2007 8:35 AM To: histonet@lists.utsouthwestern.edu Subject: {SPAM?} Re: [Histonet] I think I will give it an acronym I'm all about ThermoFisher, since Fisher was started in Pittsburgh. go steelers! (I've been assimilated into the city, not being a true Pittsburgher.) I'll take your allyourbase reference and up it one chocolate rain: chocolate rain thermofisher has no sense of shame Emily -- Remember our war hysteria, when we called sauerkraut 'Liberty cabbage' and somebody actually proposed calling German measles, 'Liberty measles?'...Remember when the hick legislators in certain states, in obedience to William Jennings Bryan, who learned his biology from his pious old grandma, set up shop as scientific experts and made the whole world laugh itself sick by forbidding the teaching of evolution? --Sinclair Lewis, It Can't Happen Here, 1935 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Building a website is a piece of cake. Yahoo! Small Business gives you all the tools to get online. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic message and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. Dissemination, forwarding, printing or copying of this message/documents without the consent of the sender is prohibited. From rjbuesa <@t> yahoo.com Tue Sep 18 10:04:58 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Sep 18 10:05:11 2007 Subject: [Histonet] Recyclers In-Reply-To: Message-ID: <277104.15512.qm@web61219.mail.yahoo.com> Toni: I used for years a B/R (evaporator/condenser) unit and it worked like a "horse". Efficient in recycling xylene, but it required twice the time to recycle ethanol, so I decided to recycle xylene only. If you inted to recycle formalin (something I would advise against!), it would have to be one "chemical recycler" (those chemical purifiers, NEVER of the evaporator/condenser type). Ren? J. "Rathborne, Toni" wrote: I'd like some feedback from anyone who has had experience with solvent recyclers. I have literature on the CBG and B/R. What other ones are out there? Is there one company that is efficient at recycling alcohol, xylene and formalin? Thanks in advance for your comments, Toni Rathborne CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Pinpoint customers who are looking for what you sell. From agoldstein <@t> KaiserAssociates.com Tue Sep 18 10:08:56 2007 From: agoldstein <@t> KaiserAssociates.com (Adam Goldstein) Date: Tue Sep 18 10:06:29 2007 Subject: [Histonet] Last chance! Histology Consumables Product Survey ($50 Honorarium opportunity) Message-ID: Dear Histonetters, This is your last opportunity to participate in a survey that will help shape the future of histology products and earn $50 at the same time! Please respond quickly, as we are only administering the survey for a few more days. If you have already responded, please do not do so again. Our firm, Kaiser Associates, is conducting research on the products and processes for the histologic technique of primary (H&E) staining and would like your input. By participating in this survey, you will be influencing potential upcoming changes to the market for primary (H&E) staining products. The completion of this survey should only take 20-30 minutes, and in return for your participation you will receive an honorarium of US $50 in the form of an American Express gift card. If you would like to participate in this survey please reply to AGoldstein@KaiserAssociates.com with: 1. Your job title; 2. Your department; and 3. Your company/hospital name and location Upon receipt of the above information, we will send you the web link to the survey. Please note that we are primarily interested in respondents from the US or Europe. Thanks! Sincerely, Kaiser Associates, Inc. Research Team About Kaiser Associates, Inc: Kaiser Associates is a research-based international consulting firm dedicated to helping leading global corporations develop effective strategies to drive continued operating performance. For more information please go to www.KaiserAssociates.com . From CIngles <@t> uwhealth.org Tue Sep 18 10:06:31 2007 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Tue Sep 18 10:06:44 2007 Subject: {SPAM?} Re: [Histonet] I think I will give it an acronym References: <304140.51720.qm@web53610.mail.re2.yahoo.com> Message-ID: <08A0A863637F1349BBFD83A96B27A50A120078@uwhis-xchng3.uwhis.hosp.wisc.edu> Go Larry! WE own our team. Who else can say that! That, and who has the QB who keeps breaking all the League Records?!? I would also like to ask forbearance of our collegues across the pond regarding this thread. I think there is a reason the most popular game on both sides of the pond is referred to as Football. :) Claire Ingles ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Larry Woody Sent: Tue 9/18/2007 9:54 AM To: Douglas D Deltour; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym The best fans, the only true fans, and America's team, The Green Bay Packers! Need I say more. Douglas D Deltour wrote: Did you have to mention football? Do you know how much I dislike Steelers fans? Almost as much as Patriot fans. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, September 18, 2007 8:35 AM To: histonet@lists.utsouthwestern.edu Subject: {SPAM?} Re: [Histonet] I think I will give it an acronym I'm all about ThermoFisher, since Fisher was started in Pittsburgh. go steelers! (I've been assimilated into the city, not being a true Pittsburgher.) I'll take your allyourbase reference and up it one chocolate rain: chocolate rain thermofisher has no sense of shame Emily -- Remember our war hysteria, when we called sauerkraut 'Liberty cabbage' and somebody actually proposed calling German measles, 'Liberty measles?'...Remember when the hick legislators in certain states, in obedience to William Jennings Bryan, who learned his biology from his pious old grandma, set up shop as scientific experts and made the whole world laugh itself sick by forbidding the teaching of evolution? --Sinclair Lewis, It Can't Happen Here, 1935 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Building a website is a piece of cake. Yahoo! Small Business gives you all the tools to get online. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From m5johnso <@t> meded.ucsd.edu Tue Sep 18 10:19:30 2007 From: m5johnso <@t> meded.ucsd.edu (Mindy Johnson) Date: Tue Sep 18 10:19:47 2007 Subject: {SPAM?} Re: [Histonet] I think I will give it an acronym In-Reply-To: <08A0A863637F1349BBFD83A96B27A50A120078@uwhis-xchng3.uwhis.hosp.wisc.edu> References: <304140.51720.qm@web53610.mail.re2.yahoo.com> <08A0A863637F1349BBFD83A96B27A50A120078@uwhis-xchng3.uwhis.hosp.wisc.edu> Message-ID: <006201c7fa07$4fae0c30$ef0a2490$@ucsd.edu> I don't understand the big hype around the Packers. Seriously..... oh wait that's why cause I grew up in MINNESOTA!!! So, I'll have to argue that the Packers are soooo good. I know we've beat you a few times, even when we suck!! ?Mindy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Tuesday, September 18, 2007 8:07 AM To: histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym Go Larry! WE own our team. Who else can say that! That, and who has the QB who keeps breaking all the League Records?!? I would also like to ask forbearance of our collegues across the pond regarding this thread. I think there is a reason the most popular game on both sides of the pond is referred to as Football. :) Claire Ingles ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Larry Woody Sent: Tue 9/18/2007 9:54 AM To: Douglas D Deltour; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym The best fans, the only true fans, and America's team, The Green Bay Packers! Need I say more. Douglas D Deltour wrote: Did you have to mention football? Do you know how much I dislike Steelers fans? Almost as much as Patriot fans. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, September 18, 2007 8:35 AM To: histonet@lists.utsouthwestern.edu Subject: {SPAM?} Re: [Histonet] I think I will give it an acronym I'm all about ThermoFisher, since Fisher was started in Pittsburgh. go steelers! (I've been assimilated into the city, not being a true Pittsburgher.) I'll take your allyourbase reference and up it one chocolate rain: chocolate rain thermofisher has no sense of shame Emily -- Remember our war hysteria, when we called sauerkraut 'Liberty cabbage' and somebody actually proposed calling German measles, 'Liberty measles?'...Remember when the hick legislators in certain states, in obedience to William Jennings Bryan, who learned his biology from his pious old grandma, set up shop as scientific experts and made the whole world laugh itself sick by forbidding the teaching of evolution? --Sinclair Lewis, It Can't Happen Here, 1935 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Building a website is a piece of cake. Yahoo! Small Business gives you all the tools to get online. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Tue Sep 18 11:19:36 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue Sep 18 10:20:13 2007 Subject: {SPAM?} Re: [Histonet] I think I will give it an acronym In-Reply-To: <003b01c7f9fe$62c84550$6701a8c0@CHERYLSLAPTOP> Message-ID: I am a lifetime Chargers fan so now you know why I hate the Steelers and the Patriots. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: Cheryl R. Kerry [mailto:tkngflght@yahoo.com] Sent: Tuesday, September 18, 2007 9:16 AM To: 'Douglas D Deltour'; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym Oh Doug--you and I can no longer be friends!!! Born and raised a Steeler fan--in fact, if you don't say it 'STILLERS' you ain't a real fan!! (Go Emily!!) Don't even get me started on the Pirates and the Pens!! Cheryl Cheryl R. Kerry, HT(ASCP) Full Staff Inc. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, September 18, 2007 9:51 AM To: 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym Did you have to mention football? Do you know how much I dislike Steelers fans? Almost as much as Patriot fans. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, September 18, 2007 8:35 AM To: histonet@lists.utsouthwestern.edu Subject: {SPAM?} Re: [Histonet] I think I will give it an acronym I'm all about ThermoFisher, since Fisher was started in Pittsburgh. go steelers! (I've been assimilated into the city, not being a true Pittsburgher.) I'll take your allyourbase reference and up it one chocolate rain: chocolate rain thermofisher has no sense of shame Emily -- Remember our war hysteria, when we called sauerkraut 'Liberty cabbage' and somebody actually proposed calling German measles, 'Liberty measles?'...Remember when the hick legislators in certain states, in obedience to William Jennings Bryan, who learned his biology from his pious old grandma, set up shop as scientific experts and made the whole world laugh itself sick by forbidding the teaching of evolution? --Sinclair Lewis, It Can't Happen Here, 1935 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Tue Sep 18 11:23:48 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue Sep 18 10:24:31 2007 Subject: {SPAM?} Re: [Histonet] I think I will give it an acronym In-Reply-To: Message-ID: Are you out of your mind? - YES Is it that cold in SC? - Currently 72 so YES Did you feel a need to be flamed and burned to a crisp? - By Steeler fans? Come on. GO CHARGERS! Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. _____ From: Dolores Townsend [mailto:TownsendD@childrensdayton.org] Sent: Tuesday, September 18, 2007 9:35 AM To: 'Emily Sours'; histonet@lists.utsouthwestern.edu; Douglas D Deltour Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym Douglas, Douglas, Douglas Are you out of your mind? Is it that cold in SC? Did you feel a need to be flamed and burned to a crisp? I am not a big fan of football myself, being a woman and European to boot, but I wouldn't dream to go there... Dolores From slappycraw <@t> yahoo.com Tue Sep 18 10:30:12 2007 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Tue Sep 18 10:30:24 2007 Subject: {SPAM?} Re: [Histonet] I think I will give it an acronym In-Reply-To: <006201c7fa07$4fae0c30$ef0a2490$@ucsd.edu> Message-ID: <191741.42346.qm@web53601.mail.re2.yahoo.com> The ViQueens are weak and got beat up by the lowly Lions. Once when I was in Histology school in Eau Claire Wi. I was up in Minneapolis at a party and ended up getting arrested for a dine and dash but when I told the cops that I was a Packer fan they not only let me go, but they begged me to forgive them and to please not take it out on them come Sunday. I told them I would ask Brett to only throw 5 touchdowns against them instead of 6. There's a reason why it's called titletown and the Lombardi Trophy. Mindy Johnson wrote: I don't understand the big hype around the Packers. Seriously..... oh wait that's why cause I grew up in MINNESOTA!!! So, I'll have to argue that the Packers are soooo good. I know we've beat you a few times, even when we suck!! Mindy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Tuesday, September 18, 2007 8:07 AM To: histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym Go Larry! WE own our team. Who else can say that! That, and who has the QB who keeps breaking all the League Records?!? I would also like to ask forbearance of our collegues across the pond regarding this thread. I think there is a reason the most popular game on both sides of the pond is referred to as Football. :) Claire Ingles ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Larry Woody Sent: Tue 9/18/2007 9:54 AM To: Douglas D Deltour; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym The best fans, the only true fans, and America's team, The Green Bay Packers! Need I say more. Douglas D Deltour wrote: Did you have to mention football? Do you know how much I dislike Steelers fans? Almost as much as Patriot fans. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, September 18, 2007 8:35 AM To: histonet@lists.utsouthwestern.edu Subject: {SPAM?} Re: [Histonet] I think I will give it an acronym I'm all about ThermoFisher, since Fisher was started in Pittsburgh. go steelers! (I've been assimilated into the city, not being a true Pittsburgher.) I'll take your allyourbase reference and up it one chocolate rain: chocolate rain thermofisher has no sense of shame Emily -- Remember our war hysteria, when we called sauerkraut 'Liberty cabbage' and somebody actually proposed calling German measles, 'Liberty measles?'...Remember when the hick legislators in certain states, in obedience to William Jennings Bryan, who learned his biology from his pious old grandma, set up shop as scientific experts and made the whole world laugh itself sick by forbidding the teaching of evolution? --Sinclair Lewis, It Can't Happen Here, 1935 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Building a website is a piece of cake. Yahoo! Small Business gives you all the tools to get online. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: mail, news, photos & more. From CSidell <@t> advancederm.net Tue Sep 18 10:31:54 2007 From: CSidell <@t> advancederm.net (Carol Sidell) Date: Tue Sep 18 10:33:21 2007 Subject: {SPAM?} Re: [Histonet] I think I will give it an acronym In-Reply-To: <20070918152520.E17AA8350@mail.advancederm.net> Message-ID: <4AD6A4E531E8C943A730559B6B81DF072573D8@dc.Advancederm.net> I am a lifetime Cleveland Browns fan so I really don't like the Steelers either!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, September 18, 2007 9:20 AM To: 'Cheryl R. Kerry'; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym I am a lifetime Chargers fan so now you know why I hate the Steelers and the Patriots. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: Cheryl R. Kerry [mailto:tkngflght@yahoo.com] Sent: Tuesday, September 18, 2007 9:16 AM To: 'Douglas D Deltour'; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym Oh Doug--you and I can no longer be friends!!! Born and raised a Steeler fan--in fact, if you don't say it 'STILLERS' you ain't a real fan!! (Go Emily!!) Don't even get me started on the Pirates and the Pens!! Cheryl Cheryl R. Kerry, HT(ASCP) Full Staff Inc. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, September 18, 2007 9:51 AM To: 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym Did you have to mention football? Do you know how much I dislike Steelers fans? Almost as much as Patriot fans. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, September 18, 2007 8:35 AM To: histonet@lists.utsouthwestern.edu Subject: {SPAM?} Re: [Histonet] I think I will give it an acronym I'm all about ThermoFisher, since Fisher was started in Pittsburgh. go steelers! (I've been assimilated into the city, not being a true Pittsburgher.) I'll take your allyourbase reference and up it one chocolate rain: chocolate rain thermofisher has no sense of shame Emily -- Remember our war hysteria, when we called sauerkraut 'Liberty cabbage' and somebody actually proposed calling German measles, 'Liberty measles?'...Remember when the hick legislators in certain states, in obedience to William Jennings Bryan, who learned his biology from his pious old grandma, set up shop as scientific experts and made the whole world laugh itself sick by forbidding the teaching of evolution? --Sinclair Lewis, It Can't Happen Here, 1935 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From khart <@t> gsopath.com Tue Sep 18 10:34:10 2007 From: khart <@t> gsopath.com (Kathy Hart) Date: Tue Sep 18 10:34:21 2007 Subject: [Histonet] Positions available Message-ID: <03B63DE44B5C014D84F9A9D8A968B51797CD47@lithium.corp.gsopath.com> Greensboro Pathology Associates, a multi-specialty pathology laboratory, is currently seeking experienced Histotechnicians to join our exceptional team working with surgical and dermatopathology specimens. All Histotechnicians actively participate in special stains rotation, daily quality control procedures and equipment troubleshooting in our fast paced lab. Two positions currently available (3am-11:30am, 4am - 12:30pm). Qualifications: Current HT/ASCP certification preferred. Previous HT experience is required. Please mail, fax or e-mail resumes to: Ellen Pancoast, PHR Human Resources Manager Phone (direct): 336/387-2522 Fax (direct): 336/510-0192 e-mail: epancoast@gsopath.com Thank you, Kathy S. Hart Lab Manager Greensboro Pathology Associates 706 Green Valley Rd. Suite 104 Greensboro, NC 27408 Phone: 336-387-2513 Fax: 336-387-2563 khart@gsopath.com From Pat.Bell <@t> UCHSC.edu Tue Sep 18 10:36:07 2007 From: Pat.Bell <@t> UCHSC.edu (Pat.Bell@UCHSC.edu) Date: Tue Sep 18 10:36:25 2007 Subject: {SPAM?} Re: [Histonet] I think I will give it an acronym In-Reply-To: <4AD6A4E531E8C943A730559B6B81DF072573D8@dc.Advancederm.net> Message-ID: <71FCC52823941D49A4BC39B48C6E3428F3BDF5@java.uchsc.edu> YEA BRONCOS!!!!!!! Pat Bell University of Co. Health Sciences Center Denver, Colorado -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Sidell Sent: Tuesday, September 18, 2007 9:32 AM To: Douglas D Deltour; Cheryl R. Kerry; Emily Sours; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym I am a lifetime Cleveland Browns fan so I really don't like the Steelers either!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, September 18, 2007 9:20 AM To: 'Cheryl R. Kerry'; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym I am a lifetime Chargers fan so now you know why I hate the Steelers and the Patriots. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: Cheryl R. Kerry [mailto:tkngflght@yahoo.com] Sent: Tuesday, September 18, 2007 9:16 AM To: 'Douglas D Deltour'; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym Oh Doug--you and I can no longer be friends!!! Born and raised a Steeler fan--in fact, if you don't say it 'STILLERS' you ain't a real fan!! (Go Emily!!) Don't even get me started on the Pirates and the Pens!! Cheryl Cheryl R. Kerry, HT(ASCP) Full Staff Inc. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, September 18, 2007 9:51 AM To: 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym Did you have to mention football? Do you know how much I dislike Steelers fans? Almost as much as Patriot fans. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, September 18, 2007 8:35 AM To: histonet@lists.utsouthwestern.edu Subject: {SPAM?} Re: [Histonet] I think I will give it an acronym I'm all about ThermoFisher, since Fisher was started in Pittsburgh. go steelers! (I've been assimilated into the city, not being a true Pittsburgher.) I'll take your allyourbase reference and up it one chocolate rain: chocolate rain thermofisher has no sense of shame Emily -- Remember our war hysteria, when we called sauerkraut 'Liberty cabbage' and somebody actually proposed calling German measles, 'Liberty measles?'...Remember when the hick legislators in certain states, in obedience to William Jennings Bryan, who learned his biology from his pious old grandma, set up shop as scientific experts and made the whole world laugh itself sick by forbidding the teaching of evolution? --Sinclair Lewis, It Can't Happen Here, 1935 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Tue Sep 18 10:37:07 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Tue Sep 18 10:37:56 2007 Subject: [Histonet] Goldner's Trichrome In-Reply-To: <3E94391DD24ECE418DCBA5EF74BA29940B39E4@shr-exch1.na01.crl.com> References: <3E94391DD24ECE418DCBA5EF74BA29940B39E4@shr-exch1.na01.crl.com> Message-ID: See Fred Monson's Histonet reply (undated): http://www.histosearch.com/histonet/Mar02A/RE.GoldnersTrichrome.html It looks like a slight variant of Masson's trichrome; none of the dyes used in it should give metachromatic effects. John Kiernan Anatomy, UWO London, Canada --- ----- Original Message ----- From: "Malinowski, Joanne O," Date: Monday, September 17, 2007 8:46 Subject: [Histonet] Goldner's Trichrome To: histonet@lists.utsouthwestern.edu > Hello Plastic Histonetters, > > Can anyone share the latest Goldner's Trichrome for MMA ground > sections?Ours turns out monochromatic. If not Goldner's, what is > your choice for > connective tissue for ground MMA sections? > > Thank you. > > Joanne > > Joanne Malinowski,HT ASCP > > Plastics Lab Manager, Medical Devices Division > > Charles River Laboratories > > Pathology Associates > > 15 Worman's Mill Court, Suite I > > Frederick, Maryland 21701 > > > > Phone 301-624-2034 > > Fax 301-663-8994 > > Email: joanne.malinowski@us.crl.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Terry.Marshall <@t> rothgen.nhs.uk Tue Sep 18 10:38:21 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Tue Sep 18 10:38:36 2007 Subject: [Histonet] Last chance! Histology Consumables Product Survey ($50Honorarium opportunity) Message-ID: <407F05A128805F4C879A33DBA32E618E0189505F@TRFT-EX01.xRothGen.nhs.uk> Before the Europeans spend time on this, be aware that, from the website of American Express:- "The Gift Card can be used at over a million businesses in the United States where American Express Cards are accepted." Pity you have to go to the US to cash in on it. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adam Goldstein Sent: 18 September 2007 16:09 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Last chance! Histology Consumables Product Survey ($50Honorarium opportunity) Dear Histonetters, This is your last opportunity to participate in a survey that will help shape the future of histology products and earn $50 at the same time! Please respond quickly, as we are only administering the survey for a few more days. If you have already responded, please do not do so again. Our firm, Kaiser Associates, is conducting research on the products and processes for the histologic technique of primary (H&E) staining and would like your input. By participating in this survey, you will be influencing potential upcoming changes to the market for primary (H&E) staining products. The completion of this survey should only take 20-30 minutes, and in return for your participation you will receive an honorarium of US $50 in the form of an American Express gift card. If you would like to participate in this survey please reply to AGoldstein@KaiserAssociates.com with: 1. Your job title; 2. Your department; and 3. Your company/hospital name and location Upon receipt of the above information, we will send you the web link to the survey. Please note that we are primarily interested in respondents from the US or Europe. Thanks! Sincerely, Kaiser Associates, Inc. Research Team About Kaiser Associates, Inc: Kaiser Associates is a research-based international consulting firm dedicated to helping leading global corporations develop effective strategies to drive continued operating performance. For more information please go to www.KaiserAssociates.com . _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Tue Sep 18 10:39:05 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Tue Sep 18 10:39:24 2007 Subject: {SPAM?} Re: [Histonet] I think I will give it an acronym In-Reply-To: <08A0A863637F1349BBFD83A96B27A50A120078@uwhis-xchng3.uwhis.hosp.wisc.edu> References: <08A0A863637F1349BBFD83A96B27A50A120078@uwhis-xchng3.uwhis.hosp.wisc.edu> Message-ID: THERMOPACKERS, now that's a good name for a team! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: 18 September 2007 16:07 To: histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym Go Larry! WE own our team. Who else can say that! That, and who has the QB who keeps breaking all the League Records?!? I would also like to ask forbearance of our collegues across the pond regarding this thread. I think there is a reason the most popular game on both sides of the pond is referred to as Football. :) Claire Ingles ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Larry Woody Sent: Tue 9/18/2007 9:54 AM To: Douglas D Deltour; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym The best fans, the only true fans, and America's team, The Green Bay Packers! Need I say more. Douglas D Deltour wrote: Did you have to mention football? Do you know how much I dislike Steelers fans? Almost as much as Patriot fans. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, September 18, 2007 8:35 AM To: histonet@lists.utsouthwestern.edu Subject: {SPAM?} Re: [Histonet] I think I will give it an acronym I'm all about ThermoFisher, since Fisher was started in Pittsburgh. go steelers! (I've been assimilated into the city, not being a true Pittsburgher.) I'll take your allyourbase reference and up it one chocolate rain: chocolate rain thermofisher has no sense of shame Emily -- Remember our war hysteria, when we called sauerkraut 'Liberty cabbage' and somebody actually proposed calling German measles, 'Liberty measles?'...Remember when the hick legislators in certain states, in obedience to William Jennings Bryan, who learned his biology from his pious old grandma, set up shop as scientific experts and made the whole world laugh itself sick by forbidding the teaching of evolution? --Sinclair Lewis, It Can't Happen Here, 1935 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Building a website is a piece of cake. Yahoo! Small Business gives you all the tools to get online. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Tue Sep 18 10:41:50 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Tue Sep 18 10:42:06 2007 Subject: [Histonet] Goldner's Trichrome Message-ID: <407F05A128805F4C879A33DBA32E618E01895060@TRFT-EX01.xRothGen.nhs.uk> I took the term monochromatic to mean there was just one colour (like our H&Es) rather than contrasting with metachromatic. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Kiernan Sent: 18 September 2007 16:37 To: Malinowski, Joanne O, Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Goldner's Trichrome See Fred Monson's Histonet reply (undated): http://www.histosearch.com/histonet/Mar02A/RE.GoldnersTrichrome.html It looks like a slight variant of Masson's trichrome; none of the dyes used in it should give metachromatic effects. John Kiernan Anatomy, UWO London, Canada --- ----- Original Message ----- From: "Malinowski, Joanne O," Date: Monday, September 17, 2007 8:46 Subject: [Histonet] Goldner's Trichrome To: histonet@lists.utsouthwestern.edu > Hello Plastic Histonetters, > > Can anyone share the latest Goldner's Trichrome for MMA ground > sections?Ours turns out monochromatic. If not Goldner's, what is your > choice for connective tissue for ground MMA sections? > > Thank you. > > Joanne > > Joanne Malinowski,HT ASCP > > Plastics Lab Manager, Medical Devices Division > > Charles River Laboratories > > Pathology Associates > > 15 Worman's Mill Court, Suite I > > Frederick, Maryland 21701 > > > > Phone 301-624-2034 > > Fax 301-663-8994 > > Email: joanne.malinowski@us.crl.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MLashus <@t> pathgroup.com Tue Sep 18 10:45:00 2007 From: MLashus <@t> pathgroup.com (Mighnon Lashus) Date: Tue Sep 18 10:44:26 2007 Subject: [Histonet] Go Pats!! Message-ID: <197CD0B02A81F94994A285C59C8AE05C09D03ED1@pgnexchange.pathgroup.com> Go Pats and Titans! What a combination. Mighnon Lashus, HT (ASCP) PathGroup Lab 4071 S. Access Road, Suite 107 Chattanooga, TN 37406 423-493-0207 423-493-0208 fax mlashus@pathgroup.com ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-221-4431. Thank you From asachau <@t> titanmed.com Tue Sep 18 10:43:17 2007 From: asachau <@t> titanmed.com (April Sachau) Date: Tue Sep 18 10:45:27 2007 Subject: [Histonet] Broncos/Packers In-Reply-To: <71FCC52823941D49A4BC39B48C6E3428F3BDF5@java.uchsc.edu> Message-ID: <7E3ACD48BA6E26408F3188FBF08693F7C3F995@titansbs1.corp.titanmed.com> Does anyone have 2 tickets that they are trying to get rid of...??? Looking to snag some... they are practically sold out... April Sachau Titan Medical Group Staff Supervisor Phone (866) 332-9600 Ext. 1023 Fax (402) 332-5181 asachau@titanmed.com see us on the web at www.titanmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pat.Bell@UCHSC.edu Sent: Tuesday, September 18, 2007 10:36 AM To: CSidell@advancederm.net; doug@ppspath.com; tkngflght@yahoo.com; talulahgosh@gmail.com; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym YEA BRONCOS!!!!!!! Pat Bell University of Co. Health Sciences Center Denver, Colorado -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Sidell Sent: Tuesday, September 18, 2007 9:32 AM To: Douglas D Deltour; Cheryl R. Kerry; Emily Sours; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym I am a lifetime Cleveland Browns fan so I really don't like the Steelers either!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, September 18, 2007 9:20 AM To: 'Cheryl R. Kerry'; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym I am a lifetime Chargers fan so now you know why I hate the Steelers and the Patriots. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: Cheryl R. Kerry [mailto:tkngflght@yahoo.com] Sent: Tuesday, September 18, 2007 9:16 AM To: 'Douglas D Deltour'; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym Oh Doug--you and I can no longer be friends!!! Born and raised a Steeler fan--in fact, if you don't say it 'STILLERS' you ain't a real fan!! (Go Emily!!) Don't even get me started on the Pirates and the Pens!! Cheryl Cheryl R. Kerry, HT(ASCP) Full Staff Inc. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, September 18, 2007 9:51 AM To: 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym Did you have to mention football? Do you know how much I dislike Steelers fans? Almost as much as Patriot fans. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, September 18, 2007 8:35 AM To: histonet@lists.utsouthwestern.edu Subject: {SPAM?} Re: [Histonet] I think I will give it an acronym I'm all about ThermoFisher, since Fisher was started in Pittsburgh. go steelers! (I've been assimilated into the city, not being a true Pittsburgher.) I'll take your allyourbase reference and up it one chocolate rain: chocolate rain thermofisher has no sense of shame Emily -- Remember our war hysteria, when we called sauerkraut 'Liberty cabbage' and somebody actually proposed calling German measles, 'Liberty measles?'...Remember when the hick legislators in certain states, in obedience to William Jennings Bryan, who learned his biology from his pious old grandma, set up shop as scientific experts and made the whole world laugh itself sick by forbidding the teaching of evolution? --Sinclair Lewis, It Can't Happen Here, 1935 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From valeria.berno <@t> embl.it Tue Sep 18 10:52:16 2007 From: valeria.berno <@t> embl.it (Valeria Berno) Date: Tue Sep 18 10:52:14 2007 Subject: [Histonet] notch-1 Message-ID: Hello All, I was wondering if there is anyone out there who has successfully done Notch-1 staining on paraffin sections. If there is, would they be able to could you tell me of the antibody, fixation/embedding protocol, and subsequent treatments, if any. I have tried 3 Santa cruz antibodies unsuccessfully and we were wondering if it is something about the antibodies or the protocol!!! Thanks a lot Valeria Berno Valeria, PhD EMBL Monterotondo Outstation via Ramarini 32 00015 Monterotondo Scalo (RM) Italy Tel: +39 06 90091 287 Fax: +39 06 90091 272 HYPERLINK "mailto:valeria.berno@embl.it"valeria.berno@embl.it No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.5.487 / Virus Database: 269.13.22/1013 - Release Date: 17/09/2007 13.29 From b-frederick <@t> northwestern.edu Tue Sep 18 10:56:28 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Tue Sep 18 10:56:47 2007 Subject: [Histonet] Last chance! Histology Consumables Product Survey($50Honorarium opportunity) In-Reply-To: <407F05A128805F4C879A33DBA32E618E0189505F@TRFT-EX01.xRothGen.nhs.uk> Message-ID: <000d01c7fa0c$7c921b10$d00f7ca5@lurie.northwestern.edu> What if you order from their catalogue? Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr, Consultant Histopathologist Sent: Tuesday, September 18, 2007 10:38 AM To: Adam Goldstein; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Last chance! Histology Consumables Product Survey($50Honorarium opportunity) Before the Europeans spend time on this, be aware that, from the website of American Express:- "The Gift Card can be used at over a million businesses in the United States where American Express Cards are accepted." Pity you have to go to the US to cash in on it. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adam Goldstein Sent: 18 September 2007 16:09 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Last chance! Histology Consumables Product Survey ($50Honorarium opportunity) Dear Histonetters, This is your last opportunity to participate in a survey that will help shape the future of histology products and earn $50 at the same time! Please respond quickly, as we are only administering the survey for a few more days. If you have already responded, please do not do so again. Our firm, Kaiser Associates, is conducting research on the products and processes for the histologic technique of primary (H&E) staining and would like your input. By participating in this survey, you will be influencing potential upcoming changes to the market for primary (H&E) staining products. The completion of this survey should only take 20-30 minutes, and in return for your participation you will receive an honorarium of US $50 in the form of an American Express gift card. If you would like to participate in this survey please reply to AGoldstein@KaiserAssociates.com with: 1. Your job title; 2. Your department; and 3. Your company/hospital name and location Upon receipt of the above information, we will send you the web link to the survey. Please note that we are primarily interested in respondents from the US or Europe. Thanks! Sincerely, Kaiser Associates, Inc. Research Team About Kaiser Associates, Inc: Kaiser Associates is a research-based international consulting firm dedicated to helping leading global corporations develop effective strategies to drive continued operating performance. For more information please go to www.KaiserAssociates.com . _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BMierow <@t> smdc.org Tue Sep 18 11:25:36 2007 From: BMierow <@t> smdc.org (Mierow, Brett T.) Date: Tue Sep 18 11:26:00 2007 Subject: [Histonet] Broncos/Packers In-Reply-To: <7E3ACD48BA6E26408F3188FBF08693F7C3F995@titansbs1.corp.titanmed.com> Message-ID: <8514F5095A68704AB5C50BCE5F783D420205C29C@SCREECH.ntcampus.smdc.org> Practically sold out.......If the game would be in Green Bay, it would have sold out 23 years ago. GO PACK! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of April Sachau Sent: Tuesday, September 18, 2007 10:43 AM To: Pat.Bell@UCHSC.edu; CSidell@advancederm.net; doug@ppspath.com; tkngflght@yahoo.com; talulahgosh@gmail.com; histonet@lists.utsouthwestern.edu Subject: [Histonet] Broncos/Packers Does anyone have 2 tickets that they are trying to get rid of...??? Looking to snag some... they are practically sold out... April Sachau Titan Medical Group Staff Supervisor Phone (866) 332-9600 Ext. 1023 Fax (402) 332-5181 asachau@titanmed.com see us on the web at www.titanmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pat.Bell@UCHSC.edu Sent: Tuesday, September 18, 2007 10:36 AM To: CSidell@advancederm.net; doug@ppspath.com; tkngflght@yahoo.com; talulahgosh@gmail.com; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym YEA BRONCOS!!!!!!! Pat Bell University of Co. Health Sciences Center Denver, Colorado -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Sidell Sent: Tuesday, September 18, 2007 9:32 AM To: Douglas D Deltour; Cheryl R. Kerry; Emily Sours; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym I am a lifetime Cleveland Browns fan so I really don't like the Steelers either!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, September 18, 2007 9:20 AM To: 'Cheryl R. Kerry'; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym I am a lifetime Chargers fan so now you know why I hate the Steelers and the Patriots. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: Cheryl R. Kerry [mailto:tkngflght@yahoo.com] Sent: Tuesday, September 18, 2007 9:16 AM To: 'Douglas D Deltour'; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym Oh Doug--you and I can no longer be friends!!! Born and raised a Steeler fan--in fact, if you don't say it 'STILLERS' you ain't a real fan!! (Go Emily!!) Don't even get me started on the Pirates and the Pens!! Cheryl Cheryl R. Kerry, HT(ASCP) Full Staff Inc. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, September 18, 2007 9:51 AM To: 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym Did you have to mention football? Do you know how much I dislike Steelers fans? Almost as much as Patriot fans. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, September 18, 2007 8:35 AM To: histonet@lists.utsouthwestern.edu Subject: {SPAM?} Re: [Histonet] I think I will give it an acronym I'm all about ThermoFisher, since Fisher was started in Pittsburgh. go steelers! (I've been assimilated into the city, not being a true Pittsburgher.) I'll take your allyourbase reference and up it one chocolate rain: chocolate rain thermofisher has no sense of shame Emily -- Remember our war hysteria, when we called sauerkraut 'Liberty cabbage' and somebody actually proposed calling German measles, 'Liberty measles?'...Remember when the hick legislators in certain states, in obedience to William Jennings Bryan, who learned his biology from his pious old grandma, set up shop as scientific experts and made the whole world laugh itself sick by forbidding the teaching of evolution? --Sinclair Lewis, It Can't Happen Here, 1935 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From ryaskovich <@t> dir.nidcr.nih.gov Tue Sep 18 11:44:10 2007 From: ryaskovich <@t> dir.nidcr.nih.gov (Yaskovich, Ruth A (NIH/NIDCR) [E]) Date: Tue Sep 18 11:44:21 2007 Subject: [Histonet] RAVENS Message-ID: Go RAVENS! From LSebree <@t> uwhealth.org Tue Sep 18 11:55:53 2007 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Tue Sep 18 11:56:06 2007 Subject: [Histonet] notch-1 In-Reply-To: Message-ID: Hi Valeria, I have tried 2 of Santa Cruz's NOTCH-1 abs with no success on FFPE sections (#s 6014 and 9170) but they don't claim to work for this application or not with my system, i.e. raised in goat. The one my researcher DIDN'T buy is the 6014-R (raised in rabbit) that might have actually worked with my system. At my researcher's insistence, I did a 1:5 dilution, 10" HIER in a pressure cooker and amplification of the primary ab. All I got was nicely delineated epithelial cells against a nasty brown background but no differentiation between normal and malignant prostate cells, which was the focus of his research. Good luck!. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Valeria Berno Sent: Tuesday, September 18, 2007 10:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] notch-1 Hello All, I was wondering if there is anyone out there who has successfully done Notch-1 staining on paraffin sections. If there is, would they be able to could you tell me of the antibody, fixation/embedding protocol, and subsequent treatments, if any. I have tried 3 Santa cruz antibodies unsuccessfully and we were wondering if it is something about the antibodies or the protocol!!! Thanks a lot Valeria Berno Valeria, PhD EMBL Monterotondo Outstation via Ramarini 32 00015 Monterotondo Scalo (RM) Italy Tel: +39 06 90091 287 Fax: +39 06 90091 272 HYPERLINK "mailto:valeria.berno@embl.it"valeria.berno@embl.it No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.5.487 / Virus Database: 269.13.22/1013 - Release Date: 17/09/2007 13.29 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tjasper <@t> copc.net Tue Sep 18 12:21:47 2007 From: tjasper <@t> copc.net (Thomas Jasper) Date: Tue Sep 18 12:22:03 2007 Subject: [Histonet] Broncos/Packers References: <8514F5095A68704AB5C50BCE5F783D420205C29C@SCREECH.ntcampus.smdc.org> Message-ID: <90354A475B420441B2A0396E5008D4965E1FA6@copc-sbs.COPC.local> You got that right. I may be in Oregon but my NFL heart will always be with Green Bay. I don't expect everyone to love them (after all we gotta play the games). But there is no denying that a team owned by the people as opposed to a single guy, no matter how benevolent or malevolent (insert your favorite owner here) is worthy of admiration. Also, if any of you NFL fans get a chance to go to Lambeau Field do it. It should be listed on the 1000 places to visit before you die. Lambeau has always been great and after it's recent renovation you are never more than 5 minutes away from the 3 B's a beer, a brat or a bathroom. Now that I'm here it's finally dawned on me that the Oregon Ducks resemble the Packers...things that make you go hmmmm! GO PACK! Relocated Cheesehead, Thomas Jasper Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 tjasper@copc.net 541/693-2677 ext. 4050 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mierow, Brett T. Sent: Tuesday, September 18, 2007 9:26 AM To: April Sachau; Pat.Bell@UCHSC.edu; CSidell@advancederm.net; doug@ppspath.com; tkngflght@yahoo.com; talulahgosh@gmail.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Broncos/Packers Practically sold out.......If the game would be in Green Bay, it would have sold out 23 years ago. GO PACK! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of April Sachau Sent: Tuesday, September 18, 2007 10:43 AM To: Pat.Bell@UCHSC.edu; CSidell@advancederm.net; doug@ppspath.com; tkngflght@yahoo.com; talulahgosh@gmail.com; histonet@lists.utsouthwestern.edu Subject: [Histonet] Broncos/Packers Does anyone have 2 tickets that they are trying to get rid of...??? Looking to snag some... they are practically sold out... April Sachau Titan Medical Group Staff Supervisor Phone (866) 332-9600 Ext. 1023 Fax (402) 332-5181 asachau@titanmed.com see us on the web at www.titanmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pat.Bell@UCHSC.edu Sent: Tuesday, September 18, 2007 10:36 AM To: CSidell@advancederm.net; doug@ppspath.com; tkngflght@yahoo.com; talulahgosh@gmail.com; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym YEA BRONCOS!!!!!!! Pat Bell University of Co. Health Sciences Center Denver, Colorado -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Sidell Sent: Tuesday, September 18, 2007 9:32 AM To: Douglas D Deltour; Cheryl R. Kerry; Emily Sours; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym I am a lifetime Cleveland Browns fan so I really don't like the Steelers either!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, September 18, 2007 9:20 AM To: 'Cheryl R. Kerry'; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym I am a lifetime Chargers fan so now you know why I hate the Steelers and the Patriots. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: Cheryl R. Kerry [mailto:tkngflght@yahoo.com] Sent: Tuesday, September 18, 2007 9:16 AM To: 'Douglas D Deltour'; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym Oh Doug--you and I can no longer be friends!!! Born and raised a Steeler fan--in fact, if you don't say it 'STILLERS' you ain't a real fan!! (Go Emily!!) Don't even get me started on the Pirates and the Pens!! Cheryl Cheryl R. Kerry, HT(ASCP) Full Staff Inc. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, September 18, 2007 9:51 AM To: 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym Did you have to mention football? Do you know how much I dislike Steelers fans? Almost as much as Patriot fans. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, September 18, 2007 8:35 AM To: histonet@lists.utsouthwestern.edu Subject: {SPAM?} Re: [Histonet] I think I will give it an acronym I'm all about ThermoFisher, since Fisher was started in Pittsburgh. go steelers! (I've been assimilated into the city, not being a true Pittsburgher.) I'll take your allyourbase reference and up it one chocolate rain: chocolate rain thermofisher has no sense of shame Emily -- Remember our war hysteria, when we called sauerkraut 'Liberty cabbage' and somebody actually proposed calling German measles, 'Liberty measles?'...Remember when the hick legislators in certain states, in obedience to William Jennings Bryan, who learned his biology from his pious old grandma, set up shop as scientific experts and made the whole world laugh itself sick by forbidding the teaching of evolution? --Sinclair Lewis, It Can't Happen Here, 1935 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJJ <@t> Stowers-Institute.org Tue Sep 18 12:27:39 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Tue Sep 18 12:28:06 2007 Subject: [Histonet] Re: notch-1 In-Reply-To: Message-ID: In response to Valeria's post: >>Hello All, I was wondering if there is anyone out there who has successfully done Notch-1 staining on paraffin sections. If there is, would they be able to could you tell me of the antibody, fixation/embedding protocol, and subsequent treatments, if any. I have tried 3 Santa cruz antibodies unsuccessfully and we were wondering if it is something about the antibodies or the protocol!!! Thanks a lot Valeria Berno Valeria, PhD<< We have had some success using cleaved Notch-1 (Cell Signaling, V1744) using Bouin's fixed E9 mouse embryo, and immunostaining with tyramide amplification. We originally got the materials and methods from Raphael Kopan's lab at Wash. University in St. Louis. We were never able to get staining in formalin fixed material. I think it's difficult stuff to get to consistently work in paraffin embedded materials. Good luck! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From doug <@t> ppspath.com Tue Sep 18 13:32:00 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue Sep 18 12:32:38 2007 Subject: {SPAM?} RE: [Histonet] Broncos/Packers In-Reply-To: <90354A475B420441B2A0396E5008D4965E1FA6@copc-sbs.COPC.local> Message-ID: Speaking of the Packers.... I guess you guys will enjoy the butt whipping they will receive this Sunday. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Tuesday, September 18, 2007 12:22 PM To: Mierow, Brett T. Cc: histonet@lists.utsouthwestern.edu Subject: {SPAM?} RE: [Histonet] Broncos/Packers You got that right. I may be in Oregon but my NFL heart will always be with Green Bay. I don't expect everyone to love them (after all we gotta play the games). But there is no denying that a team owned by the people as opposed to a single guy, no matter how benevolent or malevolent (insert your favorite owner here) is worthy of admiration. Also, if any of you NFL fans get a chance to go to Lambeau Field do it. It should be listed on the 1000 places to visit before you die. Lambeau has always been great and after it's recent renovation you are never more than 5 minutes away from the 3 B's a beer, a brat or a bathroom. Now that I'm here it's finally dawned on me that the Oregon Ducks resemble the Packers...things that make you go hmmmm! GO PACK! Relocated Cheesehead, Thomas Jasper Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 tjasper@copc.net 541/693-2677 ext. 4050 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mierow, Brett T. Sent: Tuesday, September 18, 2007 9:26 AM To: April Sachau; Pat.Bell@UCHSC.edu; CSidell@advancederm.net; doug@ppspath.com; tkngflght@yahoo.com; talulahgosh@gmail.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Broncos/Packers Practically sold out.......If the game would be in Green Bay, it would have sold out 23 years ago. GO PACK! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of April Sachau Sent: Tuesday, September 18, 2007 10:43 AM To: Pat.Bell@UCHSC.edu; CSidell@advancederm.net; doug@ppspath.com; tkngflght@yahoo.com; talulahgosh@gmail.com; histonet@lists.utsouthwestern.edu Subject: [Histonet] Broncos/Packers Does anyone have 2 tickets that they are trying to get rid of...??? Looking to snag some... they are practically sold out... April Sachau Titan Medical Group Staff Supervisor Phone (866) 332-9600 Ext. 1023 Fax (402) 332-5181 asachau@titanmed.com see us on the web at www.titanmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pat.Bell@UCHSC.edu Sent: Tuesday, September 18, 2007 10:36 AM To: CSidell@advancederm.net; doug@ppspath.com; tkngflght@yahoo.com; talulahgosh@gmail.com; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym YEA BRONCOS!!!!!!! Pat Bell University of Co. Health Sciences Center Denver, Colorado -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Sidell Sent: Tuesday, September 18, 2007 9:32 AM To: Douglas D Deltour; Cheryl R. Kerry; Emily Sours; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym I am a lifetime Cleveland Browns fan so I really don't like the Steelers either!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, September 18, 2007 9:20 AM To: 'Cheryl R. Kerry'; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym I am a lifetime Chargers fan so now you know why I hate the Steelers and the Patriots. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: Cheryl R. Kerry [mailto:tkngflght@yahoo.com] Sent: Tuesday, September 18, 2007 9:16 AM To: 'Douglas D Deltour'; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym Oh Doug--you and I can no longer be friends!!! Born and raised a Steeler fan--in fact, if you don't say it 'STILLERS' you ain't a real fan!! (Go Emily!!) Don't even get me started on the Pirates and the Pens!! Cheryl Cheryl R. Kerry, HT(ASCP) Full Staff Inc. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, September 18, 2007 9:51 AM To: 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym Did you have to mention football? Do you know how much I dislike Steelers fans? Almost as much as Patriot fans. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, September 18, 2007 8:35 AM To: histonet@lists.utsouthwestern.edu Subject: {SPAM?} Re: [Histonet] I think I will give it an acronym I'm all about ThermoFisher, since Fisher was started in Pittsburgh. go steelers! (I've been assimilated into the city, not being a true Pittsburgher.) I'll take your allyourbase reference and up it one chocolate rain: chocolate rain thermofisher has no sense of shame Emily -- Remember our war hysteria, when we called sauerkraut 'Liberty cabbage' and somebody actually proposed calling German measles, 'Liberty measles?'...Remember when the hick legislators in certain states, in obedience to William Jennings Bryan, who learned his biology from his pious old grandma, set up shop as scientific experts and made the whole world laugh itself sick by forbidding the teaching of evolution? --Sinclair Lewis, It Can't Happen Here, 1935 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BMierow <@t> smdc.org Tue Sep 18 12:51:46 2007 From: BMierow <@t> smdc.org (Mierow, Brett T.) Date: Tue Sep 18 12:52:12 2007 Subject: {SPAM?} RE: [Histonet] Broncos/Packers In-Reply-To: <200709181739.l8IHdoCe007529@persephone.smdc.org> Message-ID: <8514F5095A68704AB5C50BCE5F783D420205C2A3@SCREECH.ntcampus.smdc.org> I guess the Chargers know about a good butt whipping! After Sundays game, WOW! What a crushing! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Douglas D Deltour Sent: Tuesday, September 18, 2007 1:32 PM To: 'Thomas Jasper'; Mierow, Brett T. Cc: histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} RE: [Histonet] Broncos/Packers Speaking of the Packers.... I guess you guys will enjoy the butt whipping they will receive this Sunday. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Tuesday, September 18, 2007 12:22 PM To: Mierow, Brett T. Cc: histonet@lists.utsouthwestern.edu Subject: {SPAM?} RE: [Histonet] Broncos/Packers You got that right. I may be in Oregon but my NFL heart will always be with Green Bay. I don't expect everyone to love them (after all we gotta play the games). But there is no denying that a team owned by the people as opposed to a single guy, no matter how benevolent or malevolent (insert your favorite owner here) is worthy of admiration. Also, if any of you NFL fans get a chance to go to Lambeau Field do it. It should be listed on the 1000 places to visit before you die. Lambeau has always been great and after it's recent renovation you are never more than 5 minutes away from the 3 B's a beer, a brat or a bathroom. Now that I'm here it's finally dawned on me that the Oregon Ducks resemble the Packers...things that make you go hmmmm! GO PACK! Relocated Cheesehead, Thomas Jasper Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 tjasper@copc.net 541/693-2677 ext. 4050 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mierow, Brett T. Sent: Tuesday, September 18, 2007 9:26 AM To: April Sachau; Pat.Bell@UCHSC.edu; CSidell@advancederm.net; doug@ppspath.com; tkngflght@yahoo.com; talulahgosh@gmail.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Broncos/Packers Practically sold out.......If the game would be in Green Bay, it would have sold out 23 years ago. GO PACK! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of April Sachau Sent: Tuesday, September 18, 2007 10:43 AM To: Pat.Bell@UCHSC.edu; CSidell@advancederm.net; doug@ppspath.com; tkngflght@yahoo.com; talulahgosh@gmail.com; histonet@lists.utsouthwestern.edu Subject: [Histonet] Broncos/Packers Does anyone have 2 tickets that they are trying to get rid of...??? Looking to snag some... they are practically sold out... April Sachau Titan Medical Group Staff Supervisor Phone (866) 332-9600 Ext. 1023 Fax (402) 332-5181 asachau@titanmed.com see us on the web at www.titanmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pat.Bell@UCHSC.edu Sent: Tuesday, September 18, 2007 10:36 AM To: CSidell@advancederm.net; doug@ppspath.com; tkngflght@yahoo.com; talulahgosh@gmail.com; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym YEA BRONCOS!!!!!!! Pat Bell University of Co. Health Sciences Center Denver, Colorado -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Sidell Sent: Tuesday, September 18, 2007 9:32 AM To: Douglas D Deltour; Cheryl R. Kerry; Emily Sours; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym I am a lifetime Cleveland Browns fan so I really don't like the Steelers either!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, September 18, 2007 9:20 AM To: 'Cheryl R. Kerry'; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym I am a lifetime Chargers fan so now you know why I hate the Steelers and the Patriots. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: Cheryl R. Kerry [mailto:tkngflght@yahoo.com] Sent: Tuesday, September 18, 2007 9:16 AM To: 'Douglas D Deltour'; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym Oh Doug--you and I can no longer be friends!!! Born and raised a Steeler fan--in fact, if you don't say it 'STILLERS' you ain't a real fan!! (Go Emily!!) Don't even get me started on the Pirates and the Pens!! Cheryl Cheryl R. Kerry, HT(ASCP) Full Staff Inc. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, September 18, 2007 9:51 AM To: 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym Did you have to mention football? Do you know how much I dislike Steelers fans? Almost as much as Patriot fans. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, September 18, 2007 8:35 AM To: histonet@lists.utsouthwestern.edu Subject: {SPAM?} Re: [Histonet] I think I will give it an acronym I'm all about ThermoFisher, since Fisher was started in Pittsburgh. go steelers! (I've been assimilated into the city, not being a true Pittsburgher.) I'll take your allyourbase reference and up it one chocolate rain: chocolate rain thermofisher has no sense of shame Emily -- Remember our war hysteria, when we called sauerkraut 'Liberty cabbage' and somebody actually proposed calling German measles, 'Liberty measles?'...Remember when the hick legislators in certain states, in obedience to William Jennings Bryan, who learned his biology from his pious old grandma, set up shop as scientific experts and made the whole world laugh itself sick by forbidding the teaching of evolution? --Sinclair Lewis, It Can't Happen Here, 1935 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From sbreeden <@t> nmda.nmsu.edu Tue Sep 18 13:03:35 2007 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Sep 18 13:03:49 2007 Subject: [Histonet] Football and Flaming Message-ID: <4D14F0FC9316DD41972D5F03C070908B8F46AA@nmdamailsvr.nmda.ad.nmsu.edu> I've been quiet lately, mainly because as my vacation approaches (3 days!), all **** is breaking loose (that happens when one is the Only histotech). However, this Football Thing has gotten my attention. No one has said anything about America's Team - the Dallas Cowboys! Now, ya'll just have a big ol' time flaming me because my skin is so thick from years of totally unwarranted abuse by lesser teams' fans that you can't hurt me. Besides, I've given up that highly overrated physical sport for the real sport of BASEBALL - where stealth and cunning mean so much more. Go Braves!! I was further sure of my Sports Choice last year when my friend Linda Blazek and I paid way too much money for two tickets to see the D-Backs in PHX. But we had great seats! So, give me your best shot and I'll try to remember I asked for it... Vacation cures almost everything... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From Karen.Heckford <@t> CHW.edu Tue Sep 18 13:22:37 2007 From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF) Date: Tue Sep 18 13:22:53 2007 Subject: [Histonet] Go 49ers Message-ID: O' Yea! The niners are coming back you just wait. We will be adding our sixth(Yes, I said sixth) Super Bowl ring here really soon. GO NINERS!!!! I just absolutely love Football Season. I guess my husband is really lucky!!! :-) Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathology Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu From lblazek <@t> digestivespecialists.com Tue Sep 18 13:37:35 2007 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Sep 18 13:36:04 2007 Subject: {SPAM?} Re: [Histonet] I think I will give it an acronym In-Reply-To: <4AD6A4E531E8C943A730559B6B81DF072573D8@dc.Advancederm.net> References: <20070918152520.E17AA8350@mail.advancederm.net> <4AD6A4E531E8C943A730559B6B81DF072573D8@dc.Advancederm.net> Message-ID: <1F937FB30BDB7C4A9F39F83FEA8D379F3E4C2D@bruexchange1.digestivespecialists.com> GO BROWNS!!!!!! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Sidell Sent: Tuesday, September 18, 2007 11:32 AM To: Douglas D Deltour; Cheryl R. Kerry; Emily Sours; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym I am a lifetime Cleveland Browns fan so I really don't like the Steelers either!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, September 18, 2007 9:20 AM To: 'Cheryl R. Kerry'; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym I am a lifetime Chargers fan so now you know why I hate the Steelers and the Patriots. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: Cheryl R. Kerry [mailto:tkngflght@yahoo.com] Sent: Tuesday, September 18, 2007 9:16 AM To: 'Douglas D Deltour'; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym Oh Doug--you and I can no longer be friends!!! Born and raised a Steeler fan--in fact, if you don't say it 'STILLERS' you ain't a real fan!! (Go Emily!!) Don't even get me started on the Pirates and the Pens!! Cheryl Cheryl R. Kerry, HT(ASCP) Full Staff Inc. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, September 18, 2007 9:51 AM To: 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym Did you have to mention football? Do you know how much I dislike Steelers fans? Almost as much as Patriot fans. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, September 18, 2007 8:35 AM To: histonet@lists.utsouthwestern.edu Subject: {SPAM?} Re: [Histonet] I think I will give it an acronym I'm all about ThermoFisher, since Fisher was started in Pittsburgh. go steelers! (I've been assimilated into the city, not being a true Pittsburgher.) I'll take your allyourbase reference and up it one chocolate rain: chocolate rain thermofisher has no sense of shame Emily -- Remember our war hysteria, when we called sauerkraut 'Liberty cabbage' and somebody actually proposed calling German measles, 'Liberty measles?'...Remember when the hick legislators in certain states, in obedience to William Jennings Bryan, who learned his biology from his pious old grandma, set up shop as scientific experts and made the whole world laugh itself sick by forbidding the teaching of evolution? --Sinclair Lewis, It Can't Happen Here, 1935 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vazquezr <@t> ohsu.edu Tue Sep 18 13:36:34 2007 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Tue Sep 18 13:37:22 2007 Subject: [Histonet] Broncos/Packers Message-ID: Go Yankees!!;) Robyn OHSU >>> "Thomas Jasper" 9/18/2007 10:21 AM >>> You got that right. I may be in Oregon but my NFL heart will always be with Green Bay. I don't expect everyone to love them (after all we gotta play the games). But there is no denying that a team owned by the people as opposed to a single guy, no matter how benevolent or malevolent (insert your favorite owner here) is worthy of admiration. Also, if any of you NFL fans get a chance to go to Lambeau Field do it. It should be listed on the 1000 places to visit before you die. Lambeau has always been great and after it's recent renovation you are never more than 5 minutes away from the 3 B's a beer, a brat or a bathroom. Now that I'm here it's finally dawned on me that the Oregon Ducks resemble the Packers...things that make you go hmmmm! GO PACK! Relocated Cheesehead, Thomas Jasper Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 tjasper@copc.net 541/693-2677 ext. 4050 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mierow, Brett T. Sent: Tuesday, September 18, 2007 9:26 AM To: April Sachau; Pat.Bell@UCHSC.edu; CSidell@advancederm.net; doug@ppspath.com; tkngflght@yahoo.com; talulahgosh@gmail.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Broncos/Packers Practically sold out.......If the game would be in Green Bay, it would have sold out 23 years ago. GO PACK! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of April Sachau Sent: Tuesday, September 18, 2007 10:43 AM To: Pat.Bell@UCHSC.edu; CSidell@advancederm.net; doug@ppspath.com; tkngflght@yahoo.com; talulahgosh@gmail.com; histonet@lists.utsouthwestern.edu Subject: [Histonet] Broncos/Packers Does anyone have 2 tickets that they are trying to get rid of...??? Looking to snag some... they are practically sold out... April Sachau Titan Medical Group Staff Supervisor Phone (866) 332-9600 Ext. 1023 Fax (402) 332-5181 asachau@titanmed.com see us on the web at www.titanmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pat.Bell@UCHSC.edu Sent: Tuesday, September 18, 2007 10:36 AM To: CSidell@advancederm.net; doug@ppspath.com; tkngflght@yahoo.com; talulahgosh@gmail.com; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym YEA BRONCOS!!!!!!! Pat Bell University of Co. Health Sciences Center Denver, Colorado -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Sidell Sent: Tuesday, September 18, 2007 9:32 AM To: Douglas D Deltour; Cheryl R. Kerry; Emily Sours; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym I am a lifetime Cleveland Browns fan so I really don't like the Steelers either!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, September 18, 2007 9:20 AM To: 'Cheryl R. Kerry'; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym I am a lifetime Chargers fan so now you know why I hate the Steelers and the Patriots. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: Cheryl R. Kerry [mailto:tkngflght@yahoo.com] Sent: Tuesday, September 18, 2007 9:16 AM To: 'Douglas D Deltour'; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym Oh Doug--you and I can no longer be friends!!! Born and raised a Steeler fan--in fact, if you don't say it 'STILLERS' you ain't a real fan!! (Go Emily!!) Don't even get me started on the Pirates and the Pens!! Cheryl Cheryl R. Kerry, HT(ASCP) Full Staff Inc. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, September 18, 2007 9:51 AM To: 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym Did you have to mention football? Do you know how much I dislike Steelers fans? Almost as much as Patriot fans. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, September 18, 2007 8:35 AM To: histonet@lists.utsouthwestern.edu Subject: {SPAM?} Re: [Histonet] I think I will give it an acronym I'm all about ThermoFisher, since Fisher was started in Pittsburgh. go steelers! (I've been assimilated into the city, not being a true Pittsburgher.) I'll take your allyourbase reference and up it one chocolate rain: chocolate rain thermofisher has no sense of shame Emily -- Remember our war hysteria, when we called sauerkraut 'Liberty cabbage' and somebody actually proposed calling German measles, 'Liberty measles?'...Remember when the hick legislators in certain states, in obedience to William Jennings Bryan, who learned his biology from his pious old grandma, set up shop as scientific experts and made the whole world laugh itself sick by forbidding the teaching of evolution? --Sinclair Lewis, It Can't Happen Here, 1935 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Maxim_71 <@t> mail.ru Tue Sep 18 13:31:34 2007 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Tue Sep 18 13:40:52 2007 Subject: [Histonet] SAFETY IN THE HISTOLOGY LABORATORY Message-ID: <1576150269.20070918223134@mail.ru> Dear Rene! Your safety survey is very useful to us as article also. It is contain more actual detail. The participation in questioning has allowed us to take a look at condition of safety in our lab from the off side. So now we know that it is necessary to do to be safe. Thank you for opened our eyes! Sincerely, Maxim Peshkov Russia, Taganrog. From tp2 <@t> medicine.wisc.edu Tue Sep 18 13:41:44 2007 From: tp2 <@t> medicine.wisc.edu (Thomas Pier) Date: Tue Sep 18 13:42:28 2007 Subject: [Histonet] Football and Flaming Message-ID: <46EFD599020000DF0000B0B4@gwmail.medicine.wisc.edu> Sall, Ummm, where do I begin. "America's Team" still can't match the 12 time world champ Packers. That's right. 12. 12 is more than anyone else has. Roger Staubach is no Bart Starr and Troy Aikman is no Brett Favre. I'm not even going to start on that crackhead Michael Irvin. Your boy Tony Romo is from Wisconsin too. It would also seem to me that "your" Braves won the 1957 World Series over the New York Yankees before Atlanta stole them from Milwaukee. No flame, just facts, there you go. Tom Pier Proud Wisconsinite >>> "Breeden, Sara" 09/18/07 1:03 PM >>> I've been quiet lately, mainly because as my vacation approaches (3 days!), all **** is breaking loose (that happens when one is the Only histotech). However, this Football Thing has gotten my attention. No one has said anything about America's Team - the Dallas Cowboys! Now, ya'll just have a big ol' time flaming me because my skin is so thick from years of totally unwarranted abuse by lesser teams' fans that you can't hurt me. Besides, I've given up that highly overrated physical sport for the real sport of BASEBALL - where stealth and cunning mean so much more. Go Braves!! I was further sure of my Sports Choice last year when my friend Linda Blazek and I paid way too much money for two tickets to see the D-Backs in PHX. But we had great seats! So, give me your best shot and I'll try to remember I asked for it... Vacation cures almost everything... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From msatterfield <@t> parkwaysc.org Tue Sep 18 13:47:08 2007 From: msatterfield <@t> parkwaysc.org (Mari Satterfield) Date: Tue Sep 18 13:49:09 2007 Subject: [Histonet] Eosinophilic PNB In-Reply-To: Message-ID: Thought I would send this again. I think it got lost in all the fun we are having. I'm not complaining. I love to have fun too. I am just racking my brain as to what to look for. Thanks MS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Mari Satterfield Sent: Friday, September 14, 2007 1:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosinophilic PNB I wondered if anyone could give me any suggestions as to what might be the cause of random eosinophilic artifact in prostate needle biopsies. Sometimes not even the whole core is effected. Any suggestions would be greatly appreciated. MS _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nsnwl <@t> neuro.hfh.edu Tue Sep 18 13:56:58 2007 From: nsnwl <@t> neuro.hfh.edu (Nancy Lemke) Date: Tue Sep 18 13:57:30 2007 Subject: [Histonet] Football and Flaming Message-ID: <5bff2abf4ec3e0c04d64b66ee47cf346@neuro.hfh.edu> Sorry to be a party pooper but is it possible to eliminate the non histology threads from the general list and move that type of communication to direct emails between the interested parties? It feels like a low-level hijacking when Histonet becomes engulfed in one of these threads. Thanks Nancy Lemke Research Coordinator Hermelin Brain Tumor Center Henry Ford Hospital Detroit -----Original message----- From: "Thomas Pier" tp2@medicine.wisc.edu Date: Tue, 18 Sep 2007 14:44:03 -0400 To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Football and Flaming > Sall, > Ummm, where do I begin. "America's Team" still can't match the 12 time > world champ Packers. That's right. 12. 12 is more than anyone else has. > Roger Staubach is no Bart Starr and Troy Aikman is no Brett Favre. I'm > not even going to start on that crackhead Michael Irvin. Your boy Tony > Romo is from Wisconsin too. It would also seem to me that "your" Braves > won the 1957 World Series over the New York Yankees before Atlanta stole > them from Milwaukee. No flame, just facts, there you go. > > Tom Pier > Proud Wisconsinite > > >>> "Breeden, Sara" 09/18/07 1:03 PM >>> > I've been quiet lately, mainly because as my vacation approaches (3 > days!), all **** is breaking loose (that happens when one is the Only > histotech). However, this Football Thing has gotten my attention. No > one has said anything about America's Team - the Dallas Cowboys! Now, > ya'll just have a big ol' time flaming me because my skin is so thick > from years of totally unwarranted abuse by lesser teams' fans that you > can't hurt me. Besides, I've given up that highly overrated physical > sport for the real sport of BASEBALL - where stealth and cunning mean so > much more. Go Braves!! I was further sure of my Sports Choice last > year when my friend Linda Blazek and I paid way too much money for two > tickets to see the D-Backs in PHX. But we had great seats! So, give me > your best shot and I'll try to remember I asked for it... Vacation > cures almost everything... > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ============================================================================== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================== From b-frederick <@t> northwestern.edu Tue Sep 18 14:00:14 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Tue Sep 18 14:00:56 2007 Subject: {SPAM?} RE: [Histonet] Broncos/Packers In-Reply-To: <20070918173850.D2ADB55542@thepunk.it.northwestern.edu> Message-ID: <000b01c7fa26$284c51a0$d00f7ca5@lurie.northwestern.edu> I'm sure glad I'm a NASCAR fan and will be glued to the race form Dover on Sunday. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, September 18, 2007 1:32 PM To: 'Thomas Jasper'; 'Mierow, Brett T.' Cc: histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} RE: [Histonet] Broncos/Packers Speaking of the Packers.... I guess you guys will enjoy the butt whipping they will receive this Sunday. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Tuesday, September 18, 2007 12:22 PM To: Mierow, Brett T. Cc: histonet@lists.utsouthwestern.edu Subject: {SPAM?} RE: [Histonet] Broncos/Packers You got that right. I may be in Oregon but my NFL heart will always be with Green Bay. I don't expect everyone to love them (after all we gotta play the games). But there is no denying that a team owned by the people as opposed to a single guy, no matter how benevolent or malevolent (insert your favorite owner here) is worthy of admiration. Also, if any of you NFL fans get a chance to go to Lambeau Field do it. It should be listed on the 1000 places to visit before you die. Lambeau has always been great and after it's recent renovation you are never more than 5 minutes away from the 3 B's a beer, a brat or a bathroom. Now that I'm here it's finally dawned on me that the Oregon Ducks resemble the Packers...things that make you go hmmmm! GO PACK! Relocated Cheesehead, Thomas Jasper Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 tjasper@copc.net 541/693-2677 ext. 4050 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mierow, Brett T. Sent: Tuesday, September 18, 2007 9:26 AM To: April Sachau; Pat.Bell@UCHSC.edu; CSidell@advancederm.net; doug@ppspath.com; tkngflght@yahoo.com; talulahgosh@gmail.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Broncos/Packers Practically sold out.......If the game would be in Green Bay, it would have sold out 23 years ago. GO PACK! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of April Sachau Sent: Tuesday, September 18, 2007 10:43 AM To: Pat.Bell@UCHSC.edu; CSidell@advancederm.net; doug@ppspath.com; tkngflght@yahoo.com; talulahgosh@gmail.com; histonet@lists.utsouthwestern.edu Subject: [Histonet] Broncos/Packers Does anyone have 2 tickets that they are trying to get rid of...??? Looking to snag some... they are practically sold out... April Sachau Titan Medical Group Staff Supervisor Phone (866) 332-9600 Ext. 1023 Fax (402) 332-5181 asachau@titanmed.com see us on the web at www.titanmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pat.Bell@UCHSC.edu Sent: Tuesday, September 18, 2007 10:36 AM To: CSidell@advancederm.net; doug@ppspath.com; tkngflght@yahoo.com; talulahgosh@gmail.com; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym YEA BRONCOS!!!!!!! Pat Bell University of Co. Health Sciences Center Denver, Colorado -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Sidell Sent: Tuesday, September 18, 2007 9:32 AM To: Douglas D Deltour; Cheryl R. Kerry; Emily Sours; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym I am a lifetime Cleveland Browns fan so I really don't like the Steelers either!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, September 18, 2007 9:20 AM To: 'Cheryl R. Kerry'; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym I am a lifetime Chargers fan so now you know why I hate the Steelers and the Patriots. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: Cheryl R. Kerry [mailto:tkngflght@yahoo.com] Sent: Tuesday, September 18, 2007 9:16 AM To: 'Douglas D Deltour'; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym Oh Doug--you and I can no longer be friends!!! Born and raised a Steeler fan--in fact, if you don't say it 'STILLERS' you ain't a real fan!! (Go Emily!!) Don't even get me started on the Pirates and the Pens!! Cheryl Cheryl R. Kerry, HT(ASCP) Full Staff Inc. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, September 18, 2007 9:51 AM To: 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym Did you have to mention football? Do you know how much I dislike Steelers fans? Almost as much as Patriot fans. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, September 18, 2007 8:35 AM To: histonet@lists.utsouthwestern.edu Subject: {SPAM?} Re: [Histonet] I think I will give it an acronym I'm all about ThermoFisher, since Fisher was started in Pittsburgh. go steelers! (I've been assimilated into the city, not being a true Pittsburgher.) I'll take your allyourbase reference and up it one chocolate rain: chocolate rain thermofisher has no sense of shame Emily -- Remember our war hysteria, when we called sauerkraut 'Liberty cabbage' and somebody actually proposed calling German measles, 'Liberty measles?'...Remember when the hick legislators in certain states, in obedience to William Jennings Bryan, who learned his biology from his pious old grandma, set up shop as scientific experts and made the whole world laugh itself sick by forbidding the teaching of evolution? --Sinclair Lewis, It Can't Happen Here, 1935 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sjchtascp <@t> yahoo.com Tue Sep 18 14:04:35 2007 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Tue Sep 18 14:04:46 2007 Subject: [Histonet] HT position wanted So.WI/No.Ill. Message-ID: <418370.83202.qm@web38206.mail.mud.yahoo.com> I'm looking for a Histology position in the south central Wisconsin area. I live in Beloit, WI. Does anyone know of any positions available within a 50 mile radius. Thanks, Steve --------------------------------- Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. From doug <@t> ppspath.com Tue Sep 18 15:24:39 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue Sep 18 14:25:13 2007 Subject: {SPAM?} Re: [Histonet] Football and Flaming In-Reply-To: <5bff2abf4ec3e0c04d64b66ee47cf346@neuro.hfh.edu> Message-ID: Sorry Nancy. I understand that football is non-existent in Detroit. :) We will get back to the Histology Stuff. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Lemke Sent: Tuesday, September 18, 2007 1:57 PM To: Thomas Pier; histonet@lists.utsouthwestern.edu; sbreeden@nmda.nmsu.edu Subject: {SPAM?} Re: [Histonet] Football and Flaming Sorry to be a party pooper but is it possible to eliminate the non histology threads from the general list and move that type of communication to direct emails between the interested parties? It feels like a low-level hijacking when Histonet becomes engulfed in one of these threads. Thanks Nancy Lemke Research Coordinator Hermelin Brain Tumor Center Henry Ford Hospital Detroit -----Original message----- From: "Thomas Pier" tp2@medicine.wisc.edu Date: Tue, 18 Sep 2007 14:44:03 -0400 To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Football and Flaming > Sall, > Ummm, where do I begin. "America's Team" still can't match the 12 time > world champ Packers. That's right. 12. 12 is more than anyone else has. > Roger Staubach is no Bart Starr and Troy Aikman is no Brett Favre. I'm > not even going to start on that crackhead Michael Irvin. Your boy Tony > Romo is from Wisconsin too. It would also seem to me that "your" Braves > won the 1957 World Series over the New York Yankees before Atlanta stole > them from Milwaukee. No flame, just facts, there you go. > > Tom Pier > Proud Wisconsinite > > >>> "Breeden, Sara" 09/18/07 1:03 PM >>> > I've been quiet lately, mainly because as my vacation approaches (3 > days!), all **** is breaking loose (that happens when one is the Only > histotech). However, this Football Thing has gotten my attention. No > one has said anything about America's Team - the Dallas Cowboys! Now, > ya'll just have a big ol' time flaming me because my skin is so thick > from years of totally unwarranted abuse by lesser teams' fans that you > can't hurt me. Besides, I've given up that highly overrated physical > sport for the real sport of BASEBALL - where stealth and cunning mean so > much more. Go Braves!! I was further sure of my Sports Choice last > year when my friend Linda Blazek and I paid way too much money for two > tickets to see the D-Backs in PHX. But we had great seats! So, give me > your best shot and I'll try to remember I asked for it... Vacation > cures almost everything... > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ============================================================================ == CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================ == _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ryaskovich <@t> dir.nidcr.nih.gov Tue Sep 18 14:26:45 2007 From: ryaskovich <@t> dir.nidcr.nih.gov (Yaskovich, Ruth A (NIH/NIDCR) [E]) Date: Tue Sep 18 14:27:02 2007 Subject: {SPAM?} Re: [Histonet] Football and Flaming In-Reply-To: <68re31$3u5hjd@nihcesxway5.hub.nih.gov> References: <5bff2abf4ec3e0c04d64b66ee47cf346@neuro.hfh.edu> <68re31$3u5hjd@nihcesxway5.hub.nih.gov> Message-ID: Touch? Doug Ruth -----Original Message----- From: Douglas D Deltour [mailto:doug@ppspath.com] Sent: Tuesday, September 18, 2007 4:25 PM To: 'Nancy Lemke'; 'Thomas Pier'; histonet@lists.utsouthwestern.edu; sbreeden@nmda.nmsu.edu Subject: RE: {SPAM?} Re: [Histonet] Football and Flaming Sorry Nancy. I understand that football is non-existent in Detroit. :) We will get back to the Histology Stuff. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Lemke Sent: Tuesday, September 18, 2007 1:57 PM To: Thomas Pier; histonet@lists.utsouthwestern.edu; sbreeden@nmda.nmsu.edu Subject: {SPAM?} Re: [Histonet] Football and Flaming Sorry to be a party pooper but is it possible to eliminate the non histology threads from the general list and move that type of communication to direct emails between the interested parties? It feels like a low-level hijacking when Histonet becomes engulfed in one of these threads. Thanks Nancy Lemke Research Coordinator Hermelin Brain Tumor Center Henry Ford Hospital Detroit -----Original message----- From: "Thomas Pier" tp2@medicine.wisc.edu Date: Tue, 18 Sep 2007 14:44:03 -0400 To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Football and Flaming > Sall, > Ummm, where do I begin. "America's Team" still can't match the 12 time > world champ Packers. That's right. 12. 12 is more than anyone else has. > Roger Staubach is no Bart Starr and Troy Aikman is no Brett Favre. I'm > not even going to start on that crackhead Michael Irvin. Your boy Tony > Romo is from Wisconsin too. It would also seem to me that "your" Braves > won the 1957 World Series over the New York Yankees before Atlanta stole > them from Milwaukee. No flame, just facts, there you go. > > Tom Pier > Proud Wisconsinite > > >>> "Breeden, Sara" 09/18/07 1:03 PM >>> > I've been quiet lately, mainly because as my vacation approaches (3 > days!), all **** is breaking loose (that happens when one is the Only > histotech). However, this Football Thing has gotten my attention. No > one has said anything about America's Team - the Dallas Cowboys! Now, > ya'll just have a big ol' time flaming me because my skin is so thick > from years of totally unwarranted abuse by lesser teams' fans that you > can't hurt me. Besides, I've given up that highly overrated physical > sport for the real sport of BASEBALL - where stealth and cunning mean so > much more. Go Braves!! I was further sure of my Sports Choice last > year when my friend Linda Blazek and I paid way too much money for two > tickets to see the D-Backs in PHX. But we had great seats! So, give me > your best shot and I'll try to remember I asked for it... Vacation > cures almost everything... > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ============================================================================ == CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================ == _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Tue Sep 18 14:30:52 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Sep 18 14:30:17 2007 Subject: [Histonet] Goldner's Trichrome In-Reply-To: References: <3E94391DD24ECE418DCBA5EF74BA29940B39E4@shr-exch1.na01.crl.com> Message-ID: <6.0.0.22.1.20070918103001.01b2c178@gemini.msu.montana.edu> Dear All, The monochromatic effect could also be a problem since it is being done on thick, ground bone sections embedded in Methyl methacrylate. It would be better to microtome with tungsten carbide knives to obtain thin, 5 um sections,then remove the MMA completely with hot xylene or a suitable solvent, then do the Goldners. Methyl methacrylate is extremely hydrophobic and large molecular weight dyes cannot penetrate or if they do, it creates havoc with the staining. Richard Horobin wrote a wonderful article about the effects of staining on plastic sections some years back. (Sorry, reference is at home). He even discussed how Masson trichrome staining can be reversed (something some of our bone experts noticed on the on when doing this stain on plastic sections) so that blue collagen is red and the collagen stains red instead of blue with aniline blue. Surface staining is usually done with dyes like methylene blue/basic fuchsin, toluidine blue at pH 8, MacNeals tetrachrome, and Sandersons rapid bone stain from Surgipath which is a potassium permanganate oxidized methylene blue mixture, a variation of Stevenels blue. Ms. Sanderson (Mayton) figured out an easier way to make the staining solution. Mild acid etching makes this kind of staining even better since it removes a few um of calcium from surface of exposed bone so one can have better depth of dye penetration. All dyes used in these methods are of low molecular weight and can penetrate the plastic better with heat, high pH, and after etching. We preferred to do MacNeals tetrachrome for connective tissue staining on ground MMA bone sections, but is also is brilliant with toluidine blue added. Basic fuchsin can be used as a counterstain with toluidine blue and Sandersons bone stain. You very quickly learn what is connective tissue versus bone tissue components, since much of the staining is in shades of blue or green depending on what stain is used. Unfortunately, MMA embedded bone will present some problems for the ground thicker sections as staining is a bit limited as compared to microtomed MMA sections with plastic removed or decalcified bone embedded in paraffin. It is challenging but fun too. At 09:37 AM 9/18/2007, you wrote: >See Fred Monson's Histonet reply (undated): >http://www.histosearch.com/histonet/Mar02A/RE.GoldnersTrichrome.html > >It looks like a slight variant of Masson's trichrome; none of the dyes >used in it should give metachromatic effects. > >John Kiernan >Anatomy, UWO >London, Canada >--- >----- Original Message ----- >From: "Malinowski, Joanne O," >Date: Monday, September 17, 2007 8:46 >Subject: [Histonet] Goldner's Trichrome >To: histonet@lists.utsouthwestern.edu > > > Hello Plastic Histonetters, > > > > Can anyone share the latest Goldner's Trichrome for MMA ground > > sections?Ours turns out monochromatic. If not Goldner's, what is > > your choice for > > connective tissue for ground MMA sections? > > > > Thank you. > > > > Joanne > > > > Joanne Malinowski,HT ASCP > > > > Plastics Lab Manager, Medical Devices Division > > > > Charles River Laboratories > > > > Pathology Associates > > > > 15 Worman's Mill Court, Suite I > > > > Frederick, Maryland 21701 > > > > > > > > Phone 301-624-2034 > > > > Fax 301-663-8994 > > > > Email: joanne.malinowski@us.crl.com > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From vazquezr <@t> ohsu.edu Tue Sep 18 14:40:01 2007 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Tue Sep 18 14:40:29 2007 Subject: [Histonet] Football and Flaming Message-ID: Just push delete. >>> "Nancy Lemke" 9/18/2007 11:56 AM >>> Sorry to be a party pooper but is it possible to eliminate the non histology threads from the general list and move that type of communication to direct emails between the interested parties? It feels like a low-level hijacking when Histonet becomes engulfed in one of these threads. Thanks Nancy Lemke Research Coordinator Hermelin Brain Tumor Center Henry Ford Hospital Detroit -----Original message----- From: "Thomas Pier" tp2@medicine.wisc.edu Date: Tue, 18 Sep 2007 14:44:03 -0400 To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Football and Flaming > Sall, > Ummm, where do I begin. "America's Team" still can't match the 12 time > world champ Packers. That's right. 12. 12 is more than anyone else has. > Roger Staubach is no Bart Starr and Troy Aikman is no Brett Favre. I'm > not even going to start on that crackhead Michael Irvin. Your boy Tony > Romo is from Wisconsin too. It would also seem to me that "your" Braves > won the 1957 World Series over the New York Yankees before Atlanta stole > them from Milwaukee. No flame, just facts, there you go. > > Tom Pier > Proud Wisconsinite > > >>> "Breeden, Sara" 09/18/07 1:03 PM >>> > I've been quiet lately, mainly because as my vacation approaches (3 > days!), all **** is breaking loose (that happens when one is the Only > histotech). However, this Football Thing has gotten my attention. No > one has said anything about America's Team - the Dallas Cowboys! Now, > ya'll just have a big ol' time flaming me because my skin is so thick > from years of totally unwarranted abuse by lesser teams' fans that you > can't hurt me. Besides, I've given up that highly overrated physical > sport for the real sport of BASEBALL - where stealth and cunning mean so > much more. Go Braves!! I was further sure of my Sports Choice last > year when my friend Linda Blazek and I paid way too much money for two > tickets to see the D-Backs in PHX. But we had great seats! So, give me > your best shot and I'll try to remember I asked for it... Vacation > cures almost everything... > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ============================================================================== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Tue Sep 18 14:41:17 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Sep 18 14:41:27 2007 Subject: [Histonet] Football and Flaming References: <4D14F0FC9316DD41972D5F03C070908B8F46AA@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <006701c7fa2b$e20cdce0$0202a8c0@yourxhtr8hvc4p> Sally, Sally, Sally, I gave up on the Cowboys since Jerry Jones bought the team. The way he treated Tom Landry was inexcusable. Besides, I've been a die hard Pats fan since I was knee high to a grasshopper. Every time the Pats are in the Superbowl, I have a BBQ. So, maybe this year it'll be a Patriot/Cowboy game and you can mosey on down here. See, the peach cobbler was a dessert to go with the BBQ. As far as baseball, I quit watching baseball since their last strike. I can't feel sorry for someone who makes $4 million a year, but strikes for $5.5 million. On my salary, I'll be lucky to see $4 mil over my entire histo career. And this is somebody who used to skip school to go to Rex Sox games at Fenway Park. JTT ----- Original Message ----- From: "Breeden, Sara" To: Sent: Tuesday, September 18, 2007 1:03 PM Subject: [Histonet] Football and Flaming I've been quiet lately, mainly because as my vacation approaches (3 days!), all **** is breaking loose (that happens when one is the Only histotech). However, this Football Thing has gotten my attention. No one has said anything about America's Team - the Dallas Cowboys! Now, ya'll just have a big ol' time flaming me because my skin is so thick from years of totally unwarranted abuse by lesser teams' fans that you can't hurt me. Besides, I've given up that highly overrated physical sport for the real sport of BASEBALL - where stealth and cunning mean so much more. Go Braves!! I was further sure of my Sports Choice last year when my friend Linda Blazek and I paid way too much money for two tickets to see the D-Backs in PHX. But we had great seats! So, give me your best shot and I'll try to remember I asked for it... Vacation cures almost everything... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From godsgalnow <@t> aol.com Tue Sep 18 14:48:12 2007 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Tue Sep 18 14:48:41 2007 Subject: [Histonet] Re: negative controls In-Reply-To: <582736990709170940y19d73ff4re2eab837bd7a2277@mail.gmail.com> References: <582736990709152016xf425442ne92ab1589a5aac91@mail.gmail.com> <8C9C73CAE3D237F-9AC-55E@FWM-D09.sysops.aol.com> <582736990709170940y19d73ff4re2eab837bd7a2277@mail.gmail.com> Message-ID: <8C9C843FB56C718-594-DD1@FWM-D43.sysops.aol.com> >From the CAP checklist..... In general, a separate negative reagent control should be run for each block of patient tissue being immunostained; however, for cases in which there is simultaneous staining of multiple blocks from the same specimen with the same antibody (e.g., cytokeratin staining of multiple axillary sentinel lymph nodes), performing a single negative control on one of the blocks may be sufficient provided that all such blocks are fixed and processed identically.? This exception does not apply to stains on different types of tissues or those using different antigen retrieval protocols or antibody detection systems.? The laboratory director must determine which cases will have only one negative reagent control, and this must be specified in the department's procedure manual We interpret this to mean, since all of the blocks that we run IHC on are from the same specimen, the prostate, and they are getting the same IHC? antibody, PIN-4, we run one negative per patient and one positive per batch and one positive control used as a neagtive as well. Roxanne -----Original Message----- From: Amos Brooks To: godsgalnow@aol.com Cc: histonet@lists.utsouthwestern.edu Sent: Mon, 17 Sep 2007 12:40 pm Subject: Re: [Histonet] Re: negative controls Roxanne, ?? Is there any possibility that the multiple cores are from different parts of the prostate that may have metastasises from other places like kidney & liver? ?? Ultimately you can do this how you like. It is just important to know the limitations of your testing and be aware that this can bite you in the a$$ later on. Just my $0.02 Amos On 9/17/07, godsgalnow@aol.com wrote: But what if all of the blocks are prostate core biopsies? Roxanne -----Original Message----- From: Amos Brooks To: histonet@lists.utsouthwestern.edu Sent: Sat, 15 Sep 2007 11:16 pm Subject: [Histonet] Re: negative controls Hypothetically: Let's say an endometriosis case has several parts as they commonly do. A) Uterus bx (obviously), B) Ovary, C) Lg Colon bx, D) Kidney E) Liver Bx. So an IHC is ordered on the case and randomly part A is chosen as a representative negative control. Have you ever noticed how much endogenous biotin is in kidney & liver? Since there is not a negative control on it there is no real way of knowing that the labeling that one would see there is not real. Honestly having a negative on each block is best, if not for eash test being run (a tall order for sure!). Before polymer detection became popular I once used kidney as a positive control for CD10. It labels the brush boarder of the proximal convuluded tubule really nicely. Problem is that this is also a great place to find endogenous biotin! This really sold me on the use of negative controls. Thanks Mary! Have a nice day, Amos Message: 1 Date: Fri, 14 Sep 2007 15:35:48 -0400 From: godsgalnow@aol.com Subject: [Histonet] negative controls To: histonet@lists.utsouthwestern.edu Message-ID: < 8C9C51D962FF31B-284-1FE4@webmail-md17.sysops.aol.com > Content-Type: text/plain; charset="us-ascii" How many non-CAP labs out there are doing negative controls on every block that you do an antibody on? If you have a case that goes A-J and they are all 1 block each and you do an AE1/AE3 on blocks B,C,F,& J, will you do a negative on every block or just one for the entire case? This has been an ongoing debate with us.? We typincally only do 1 negative control for the case and given the above example, it might be on block A.? We ususally cut an extra on every block we cut and when the IHC is requested we just go and pull it and for the negative, we just grab whatever slide is left to run the negative on.? What is everyone else doing?? Roxanne _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Email and AIM finally together. You've gotta check out free AOL Mail! ________________________________________________________________________ Email and AIM finally together. You've gotta check out free AOL Mail! - http://mail.aol.com From slappycraw <@t> yahoo.com Tue Sep 18 15:00:12 2007 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Tue Sep 18 15:00:25 2007 Subject: {SPAM?} Re: [Histonet] Football and Flaming Message-ID: <632276.93450.qm@web53606.mail.re2.yahoo.com> Did someone from Detroit mention a party? Histology people never party, we are always business and the fact that both words are even mentioned in the same sentence makes me want to get back to work! I can't help it, my barcode is not scanning properly. Douglas D Deltour wrote: Sorry Nancy. I understand that football is non-existent in Detroit. :) We will get back to the Histology Stuff. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Lemke Sent: Tuesday, September 18, 2007 1:57 PM To: Thomas Pier; histonet@lists.utsouthwestern.edu; sbreeden@nmda.nmsu.edu Subject: {SPAM?} Re: [Histonet] Football and Flaming Sorry to be a party pooper but is it possible to eliminate the non histology threads from the general list and move that type of communication to direct emails between the interested parties? It feels like a low-level hijacking when Histonet becomes engulfed in one of these threads. Thanks Nancy Lemke Research Coordinator Hermelin Brain Tumor Center Henry Ford Hospital Detroit -----Original message----- From: "Thomas Pier" tp2@medicine.wisc.edu Date: Tue, 18 Sep 2007 14:44:03 -0400 To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Football and Flaming > Sall, > Ummm, where do I begin. "America's Team" still can't match the 12 time > world champ Packers. That's right. 12. 12 is more than anyone else has. > Roger Staubach is no Bart Starr and Troy Aikman is no Brett Favre. I'm > not even going to start on that crackhead Michael Irvin. Your boy Tony > Romo is from Wisconsin too. It would also seem to me that "your" Braves > won the 1957 World Series over the New York Yankees before Atlanta stole > them from Milwaukee. No flame, just facts, there you go. > > Tom Pier > Proud Wisconsinite > > >>> "Breeden, Sara" 09/18/07 1:03 PM >>> > I've been quiet lately, mainly because as my vacation approaches (3 > days!), all **** is breaking loose (that happens when one is the Only > histotech). However, this Football Thing has gotten my attention. No > one has said anything about America's Team - the Dallas Cowboys! Now, > ya'll just have a big ol' time flaming me because my skin is so thick > from years of totally unwarranted abuse by lesser teams' fans that you > can't hurt me. Besides, I've given up that highly overrated physical > sport for the real sport of BASEBALL - where stealth and cunning mean so > much more. Go Braves!! I was further sure of my Sports Choice last > year when my friend Linda Blazek and I paid way too much money for two > tickets to see the D-Backs in PHX. But we had great seats! So, give me > your best shot and I'll try to remember I asked for it... Vacation > cures almost everything... > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ============================================================================ == CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================ == _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Tonight's top picks. What will you watch tonight? Preview the hottest shows on Yahoo! TV. From jaroszewska.1 <@t> osu.edu Tue Sep 18 15:09:32 2007 From: jaroszewska.1 <@t> osu.edu (MARTA JAROSZEWSKA) Date: Tue Sep 18 15:09:44 2007 Subject: [Histonet] Football and Flaming Message-ID: <42216974219dc6.4219dc64221697@osu.edu> I support Nancy. This is completely impossible to "just push delee" without strong nervous reaction when there are so many unprofessional mails in Histonet. I can't waist my time. I need to read about real problems and their resolution. Have a good fun during your party but have a moderation with wriing about "crazy Friday" or "sad Monday". From JCordova <@t> omlabs.com Tue Sep 18 15:10:07 2007 From: JCordova <@t> omlabs.com (Cordova, Jean) Date: Tue Sep 18 15:11:22 2007 Subject: [Histonet] Floor in a pathology laboratory Message-ID: <18E3095CC1A30E4684F55D6D757C8D2E51646A@phoex1.peacehealth.org> We are in the process of designing a new pathology laboratory. Does anyone have suggestions on the type of flooring that we should consider? Jean Cordova Lab Manager Ext: 2137 ___________________________________________________________ This message is intended solely for the use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential, and exempt from disclosure under applicable state and federal laws. If you are not the addressee, or are not authorized to receive for the intended addressee, you are hereby notified that you may not use, copy, distribute, or disclose to anyone this message or the information contained herein. If you have received this message in error, immediately advise the sender by reply email and destroy this message. From rjbuesa <@t> yahoo.com Tue Sep 18 15:14:36 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Sep 18 15:14:48 2007 Subject: [Histonet] Floor in a pathology laboratory In-Reply-To: <18E3095CC1A30E4684F55D6D757C8D2E51646A@phoex1.peacehealth.org> Message-ID: <413123.40754.qm@web61218.mail.yahoo.com> Chemicals resistant, anti-slip, and ascrubbable to eliminate paraffin. Ren? J. "Cordova, Jean" wrote: We are in the process of designing a new pathology laboratory. Does anyone have suggestions on the type of flooring that we should consider? Jean Cordova Lab Manager Ext: 2137 ___________________________________________________________ This message is intended solely for the use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential, and exempt from disclosure under applicable state and federal laws. If you are not the addressee, or are not authorized to receive for the intended addressee, you are hereby notified that you may not use, copy, distribute, or disclose to anyone this message or the information contained herein. If you have received this message in error, immediately advise the sender by reply email and destroy this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: mail, news, photos & more. From relia1 <@t> earthlink.net Tue Sep 18 15:17:53 2007 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Sep 18 15:18:05 2007 Subject: [Histonet] RELIA Special Job Alert Great opportunity in a Dermpath setting in Boca Raton, FL Message-ID: Hello Histonetters, I hope you are all doing well today. I have a new position that I am excited to tell you about. The position is in a dermpath setting in Boca Raton. They are looking for someone with experience with MOHS, grossing and embedding. This position does require a Florida license. This is a full time dayshift position and my client offers an excellent pay rate and benefits. If you or anyone you know might be interested please contact me at 866-607-3542 or relia1@earthlink.net Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net From doug <@t> ppspath.com Tue Sep 18 16:21:46 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue Sep 18 15:22:20 2007 Subject: {SPAM?} Re: [Histonet] Football and Flaming In-Reply-To: <42216974219dc6.4219dc64221697@osu.edu> Message-ID: Dear Marta, "unprofessional mails"? Does this comment borderline at reverse sexism or are you taking a shot at our postal service? Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MARTA JAROSZEWSKA Sent: Tuesday, September 18, 2007 3:10 PM To: Robyn Vazquez Cc: histonet@lists.utsouthwestern.edu; sbreeden@nmda.nmsu.edu Subject: {SPAM?} Re: [Histonet] Football and Flaming I support Nancy. This is completely impossible to "just push delee" without strong nervous reaction when there are so many unprofessional mails in Histonet. I can't waist my time. I need to read about real problems and their resolution. Have a good fun during your party but have a moderation with wriing about "crazy Friday" or "sad Monday". _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Tue Sep 18 15:41:38 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Sep 18 15:40:58 2007 Subject: [Histonet] Floor in a pathology laboratory In-Reply-To: <413123.40754.qm@web61218.mail.yahoo.com> References: <18E3095CC1A30E4684F55D6D757C8D2E51646A@phoex1.peacehealth.org> <413123.40754.qm@web61218.mail.yahoo.com> Message-ID: <6.0.0.22.1.20070918142638.01b34e08@gemini.msu.montana.edu> I agree with Rene. We also have commerical rugs (heavy duty, very low carpet, inexpensive, and are the kind found in entry ways of buildings). We place these in front of microtome areas, sinks and processor, chairs roll on them without resistance. They can be vacuumed to pick up paraffin shavings, and when eventually trashed, merely replaced. We had an incident on a hard floor, as paraffin seems to permeate and coat all surfaces. A visiting post doc slipped, and if he hadn't grabbed a door handle, would have cracked back of his skull on our hard floor. Our janitors here would NEVER think to mop a floor, but they do vacuum these little carpets. Perhaps clinical labs have better regulations for cleaning histology lab areas than our research facility ( I work in a double wide trailer house! also called a modular) has available. If you can't use carpets, then consider the sticky peel a away mats under your microtoming areas, this catches trimmings that seem to fly around willy nilly, no matter what one does. These are also used at entry ways of areas where dust, dirt and/or prion associated work is done. Be sure you ask for acid resistant sinks, that are large, deep and enough of them to do the work necessary, staining, grossing areas, etc, etc. A lab with two sinks like ours is NOT a good situation. Also, ask that the the microtoming area has decent bright lighting and NO air vents just about the microtome area. Good luck and congratulations on being able to design a new laboratory. At 02:14 PM 9/18/2007, you wrote: >Chemicals resistant, anti-slip, and ascrubbable to eliminate paraffin. > Ren? J. > >" Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From mcauliff <@t> umdnj.edu Tue Sep 18 15:42:46 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Sep 18 15:43:35 2007 Subject: {SPAM?} Re: [Histonet] waste of bandwidth In-Reply-To: <0JOK00189Z2K9720@umduwc02.umdnj.edu> References: <0JOK00189Z2K9720@umduwc02.umdnj.edu> Message-ID: <46F03846.3040908@umdnj.edu> Dear Douglas et al: Marth meant, as I'm sure you can figure out, that "unprofessional mails" referred to the sudden avalanche of recipes, football, and what ever waste of bandwidth will be next. I was under the impression that the hardware that is used to run this list was donated by an academic institution for the purpose of scientific interchange and that other uses were not allowed. If this is going to become another list filled with idle chatter ................. Geoff Douglas D Deltour wrote: > Dear Marta, > > "unprofessional mails"? Does this comment borderline at reverse sexism or > are you taking a shot at our postal service? > > Douglas D. Deltour HT(ASCP) > Histology Manager > Professional Pathology Services, PC > One Science Court > Suite 200 > Columbia, SC 29203 > Office (803)252-1913 > Fax (803)254-3262 > Doug@ppspath.com > ***************************************************** > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of the individual or entity to > which it is addressed and may contain information that is privileged, > confidential and exempt from disclosure under applicable law. If the reader > of this message is not the intended recipient, you are hereby notified that > any dissemination, distribution, or copying of this communication is > strictly prohibited by law. If you have received this communication in > error, please notify me immediately. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MARTA > JAROSZEWSKA > Sent: Tuesday, September 18, 2007 3:10 PM > To: Robyn Vazquez > Cc: histonet@lists.utsouthwestern.edu; sbreeden@nmda.nmsu.edu > Subject: {SPAM?} Re: [Histonet] Football and Flaming > > I support Nancy. > This is completely impossible to "just push delee" without strong nervous > reaction when there are so many unprofessional mails in Histonet. I can't > waist my time. I need to read about real problems and their resolution. > Have a good fun during your party but have a moderation with wriing about > "crazy Friday" or "sad Monday". > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From alaskagirl1950 <@t> yahoo.com Tue Sep 18 15:58:03 2007 From: alaskagirl1950 <@t> yahoo.com (Patricia Adams) Date: Tue Sep 18 15:58:17 2007 Subject: {SPAM?} Re: [Histonet] Football and Flaming In-Reply-To: <632276.93450.qm@web53606.mail.re2.yahoo.com> Message-ID: <508022.93552.qm@web52501.mail.re2.yahoo.com> I know that sometimes wading through unwanted histonet "stuff" can be a trial. I feel that way when someone wants to know about some procedure I have never heard of or will never use. I do hit the delete key. Or sometimes take the time to learn something new. I also really enjoy the off topic histo threads, makes me know that all these very smart Techs are also human and would be fun to hang around with. So please keep the humor and wit going! Patrica --- Larry Woody wrote: > Did someone from Detroit mention a party? > Histology people never party, we are always > business and the fact that both words are even > mentioned in the same sentence makes me want to > get back to work! I can't help it, my barcode > is not scanning properly. > > Douglas D Deltour wrote: > Sorry Nancy. > > I understand that football is non-existent in > Detroit. :) > > We will get back to the Histology Stuff. > > Douglas D. Deltour HT(ASCP) > Histology Manager > Professional Pathology Services, PC > One Science Court > Suite 200 > Columbia, SC 29203 > Office (803)252-1913 > Fax (803)254-3262 > Doug@ppspath.com > ***************************************************** > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of > the individual or entity to > which it is addressed and may contain > information that is privileged, > confidential and exempt from disclosure under > applicable law. If the reader > of this message is not the intended recipient, > you are hereby notified that > any dissemination, distribution, or copying of > this communication is > strictly prohibited by law. If you have > received this communication in > error, please notify me immediately. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Nancy Lemke > Sent: Tuesday, September 18, 2007 1:57 PM > To: Thomas Pier; > histonet@lists.utsouthwestern.edu; > sbreeden@nmda.nmsu.edu > Subject: {SPAM?} Re: [Histonet] Football and > Flaming > > Sorry to be a party pooper but is it possible > to eliminate the non histology > threads from the general list and move that > type of communication to direct > emails between the interested parties? It feels > like a low-level hijacking > when Histonet becomes engulfed in one of these > threads. > Thanks > > Nancy Lemke > Research Coordinator > Hermelin Brain Tumor Center > Henry Ford Hospital > Detroit > -----Original message----- > From: "Thomas Pier" tp2@medicine.wisc.edu > Date: Tue, 18 Sep 2007 14:44:03 -0400 > To: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Football and Flaming > > > Sall, > > Ummm, where do I begin. "America's Team" > still can't match the 12 time > > world champ Packers. That's right. 12. 12 is > more than anyone else has. > > Roger Staubach is no Bart Starr and Troy Aikman > is no Brett Favre. I'm > > not even going to start on that crackhead > Michael Irvin. Your boy Tony > > Romo is from Wisconsin too. It would also seem > to me that "your" Braves > > won the 1957 World Series over the New York > Yankees before Atlanta stole > > them from Milwaukee. No flame, just facts, > there you go. > > > > Tom Pier > > Proud Wisconsinite > > > > >>> "Breeden, Sara" 09/18/07 1:03 PM >>> > > I've been quiet lately, mainly because as my > vacation approaches (3 > > days!), all **** is breaking loose (that > happens when one is the Only > > histotech). However, this Football Thing has > gotten my attention. No > > one has said anything about America's Team - > the Dallas Cowboys! Now, > > ya'll just have a big ol' time flaming me > because my skin is so thick > > from years of totally unwarranted abuse by > lesser teams' fans that you > > can't hurt me. Besides, I've given up that > highly overrated physical > > sport for the real sport of BASEBALL - where > stealth and cunning mean so > > much more. Go Braves!! I was further sure of > my Sports Choice last > > year when my friend Linda Blazek and I paid > way too much money for two > > tickets to see the D-Backs in PHX. But we had > great seats! So, give me > > your best shot and I'll try to remember I > asked for it... Vacation > > cures almost everything... > > > > > > > > Sally Breeden, HT(ASCP) > > > > NM Dept. of Agriculture > > > > Veterinary Diagnostic Services > > > > PO Box 4700 > > > > Albuquerque, NM 87106 > > > > 505-841-2576 > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ============================================================================ > == > CONFIDENTIALITY NOTICE: This email contains > information from the sender that > may be CONFIDENTIAL, LEGALLY PRIVILEGED, > PROPRIETARY or otherwise protected > from disclosure. This email is intended for use > only by the person or entity > to whom it is addressed. If you are not the > intended recipient, any use, > disclosure, copying, distribution, printing, or > any action taken in reliance > on the contents of this email, is strictly > prohibited. If you received this > email in error, please contact the sending > party by reply email, delete the > email from your computer system and shred any > paper copies. > > Note to Patients: There are a number of risks > you should consider before > using e-mail to communicate with us. See our > Privacy Policy and Henry Ford > My Health at www.henryford.com for more > detailed information. If you do not > believe that our policy gives you the privacy > and security protection you > need, do not send e-mail or Internet > communications to us. > > ============================================================================ > == > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Tonight's top picks. What will you watch > tonight? Preview the hottest shows on Yahoo! > TV. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ____________________________________________________________________________________ Luggage? GPS? Comic books? Check out fitting gifts for grads at Yahoo! Search http://search.yahoo.com/search?fr=oni_on_mail&p=graduation+gifts&cs=bz From shroffsm <@t> vcu.edu Tue Sep 18 16:04:23 2007 From: shroffsm <@t> vcu.edu (Seema Shroff) Date: Tue Sep 18 16:04:34 2007 Subject: [Histonet] Western Blot PDGFR alpha Western Blot and/or IHC Message-ID: Hello, Does anyone know of a good antibody to detect Platelet Derived Growth Factor Receptor - alpha subunit (PDGFR alpha) on a Western Blot and in mouse nervous tissue frozen sections. I need to do both these experiments and help on any will be appreciated. Thank you, Seema Shroff Graduate Student Anatomy & Neurobiology Virginia Commonwealth University From laurie <@t> conxis.com Tue Sep 18 16:14:35 2007 From: laurie <@t> conxis.com (laurie@conxis.com) Date: Tue Sep 18 16:14:57 2007 Subject: [Histonet] Off Topic: football tickets Message-ID: <53D8F63F087043069359A9B7EA5385C2.MAI@accuwebhosting.biz> check Ebay they occasionally have them otherwise GB is on Craigslist andsometimes people do sell them. Personally I grew up in WI and am a MN transplant and Imarried a Chicago Bears fan-atic... so I don't do NFL except by association. GO Bucky!!! I am a UW football fan ( and the marching band too, I used to work for the vet school so I got towatch band practice after work) and then there's NASCAR ( Go JR :-) ) and baseball ( GO Cubbies :-) ) Happy Tuesday, Laurie From relia1 <@t> earthlink.net Tue Sep 18 16:20:39 2007 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Sep 18 16:20:51 2007 Subject: [Histonet] Off Topic: football tickets In-Reply-To: <53D8F63F087043069359A9B7EA5385C2.MAI@accuwebhosting.biz> Message-ID: Woo hoo Finally a college football fan!!! Go Gators!!!!!!!!!!!!! Thank You! Pam M. Barker President Relia 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll Free: (866)607-3542 e-mail: relia1@earthlink.net http://home.earthlink.net/~relia1 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of laurie@conxis.com Sent: Tuesday, September 18, 2007 5:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Off Topic: football tickets check Ebay they occasionally have them otherwise GB is on Craigslist andsometimes people do sell them. Personally I grew up in WI and am a MN transplant and Imarried a Chicago Bears fan-atic... so I don't do NFL except by association. GO Bucky!!! I am a UW football fan ( and the marching band too, I used to work for the vet school so I got towatch band practice after work) and then there's NASCAR ( Go JR :-) ) and baseball ( GO Cubbies :-) ) Happy Tuesday, Laurie From kellerc2 <@t> uthscsa.edu Tue Sep 18 17:19:37 2007 From: kellerc2 <@t> uthscsa.edu (Keller, Charles) Date: Tue Sep 18 17:19:49 2007 Subject: [Histonet] Western Blot PDGFR alpha Western Blot and/or IHC In-Reply-To: References: Message-ID: Dear Seema, Abcam ab#15501 is great for IHC, westerns, and pulldowns. Charles Charles Keller, MD Assistant Professor, Department of Cellular & Structural Biology Adjunct Assistant Professor, Department of Pediatrics Children's Cancer Research Institute The University of Texas Health Science Center 7703 Floyd Curl Drive, Mail Code 7784 San Antonio, TX 78229-3900 210-562-9062 [office] 210-562-9014 [fax] http://ccri.uthscsa.edu or www.sarcomalab.org kellerc2@uthscsa.edu Postdoctoral Opportunities and Undergraduate & Summer Internships: http://ccri.uthscsa.edu/keller CCRI and UTHSCSA wish to promote open communication while protecting confidential and/or privileged information. If you have received this message in error, please inform the sender and delete all copies. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Seema Shroff Sent: Tuesday, September 18, 2007 4:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Western Blot PDGFR alpha Western Blot and/or IHC Hello, Does anyone know of a good antibody to detect Platelet Derived Growth Factor Receptor - alpha subunit (PDGFR alpha) on a Western Blot and in mouse nervous tissue frozen sections. I need to do both these experiments and help on any will be appreciated. Thank you, Seema Shroff Graduate Student Anatomy & Neurobiology Virginia Commonwealth University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Tue Sep 18 17:26:59 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Sep 18 17:26:23 2007 Subject: [Histonet] Western Blot PDGFR alpha Western Blot and/or IHC In-Reply-To: References: Message-ID: <6.0.0.22.1.20070918162544.01b44ce8@gemini.msu.montana.edu> Go to this website and do a search www.exactantigen.com It will provide a wealth of information on antibodies, and other things. At 03:04 PM 9/18/2007, you wrote: >Hello, > > > >Does anyone know of a good antibody to detect Platelet Derived Growth Factor >Receptor - alpha subunit (PDGFR alpha) on a Western Blot and in mouse >nervous tissue frozen sections. > >I need to do both these experiments and help on any will be appreciated. > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From zumbor <@t> email.cs.nsw.gov.au Tue Sep 18 18:07:49 2007 From: zumbor <@t> email.cs.nsw.gov.au (Rosalba Zumbo) Date: Tue Sep 18 18:14:46 2007 Subject: [Histonet] LIS - Autopsy Message-ID: <001d01c7fa48$bbcb2330$33616990$@cs.nsw.gov.au> Hi all, We are in the process of installing a LIS for our Histology laboratory. Can anyone recommend a system which would be suitable for a forensic histology laboratory which deals mainly with autopsy material . Thanks Rosalba Zumbo Supervisor Histology Dept Department of Forensic Medicine 42-50 Parramatt Rd Glebe NSW 2041 Australia Ph: 61 2 85847842 Fax: 61 2 95664573 zumbor@email.cs.nsw.gov.au From amosbrooks <@t> gmail.com Tue Sep 18 18:22:44 2007 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Tue Sep 18 18:22:57 2007 Subject: [Histonet] HIF1a Message-ID: <582736990709181622m4d6bc0femb6c7eeb272ed14e9@mail.gmail.com> Hi, I've been banging my head against a wall, trying to get HIF1a working. I am using NB 100-105 from Novus Biologicals. I was wondering if anyone else had any luck with this (or one from another vendor). ANY help would be appreciated. All this pulling my hair out is making me bald(er). Thanks, Amos From swelam48 <@t> gmail.com Wed Sep 19 01:48:35 2007 From: swelam48 <@t> gmail.com (wael swelam) Date: Wed Sep 19 01:48:49 2007 Subject: [Histonet] HIF1a In-Reply-To: <96566f7e0709182336n514a704ep768ff32329303fe1@mail.gmail.com> References: <582736990709181622m4d6bc0femb6c7eeb272ed14e9@mail.gmail.com> <96566f7e0709182336n514a704ep768ff32329303fe1@mail.gmail.com> Message-ID: <96566f7e0709182348u3b6db034l71b09e70b1256a3e@mail.gmail.com> Peace be upon you, I tried mouse monoclonal antibody against HIF-1 a from Chemicon International Inc. (Temecula, CA, USA, 1:100) and It was working nicely, I would recommend you to use Tris-EDTA for retrival. Good luck -- Wael Mohamed Swelam BDS (Egypt), Oral Pathology MSc (Egypt), Oral Pathology PhD (Japan), Ass. Prof. Oral Biomedical Science dept. Division of Oral Pathology, Faculty of Dentistry, King Faisal University, Dammam, Saudi Arabia, P.O: 1982/3441, http://myprofile.cos.com/wmswelam From Kemlo.Rogerson <@t> waht.swest.nhs.uk Wed Sep 19 01:53:48 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Sep 19 01:53:55 2007 Subject: {SPAM?} Re: [Histonet] I think I will give it an acronym Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222ED29@wahtntex2.waht.swest.nhs.uk> Football in America, you don't play it do you. Don't you play something called Soccer? God only knows what that is? What's that game you play where you get little diddy men and encase them in plastic with big shoulders and they sorta run at each other, then fall down? Bit like Rugby but for Wussies? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; I want to work for a company that contributes to and is part of the community. I want something not just to invest in. I want something to believe in. --Anita Roddick This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From louise.renton <@t> gmail.com Wed Sep 19 02:22:59 2007 From: louise.renton <@t> gmail.com (louise renton) Date: Wed Sep 19 02:23:28 2007 Subject: [Histonet] OT threads Message-ID: You know a little tolerance goes a long way. In the many years since the inception of Histonet, I can count on the fingers of one hand how many times the threads have gone off course. Whether or not they stay on course is a matter of opinion. Should European, Australians & South Africans all jump up & down when CLIA, CAP or HT accreditation & state licensure is mentioned and discussed at length? This has no bearing on histology in these countries. So come on guys, lighten up & put things into perspective. Remember the adage "All work & no play makes Jack a dull boy" Louise Renton (in asbestos suit)* * do not try this in your lab without prior consultation with safety officers, CAP, JHCO, etc Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From laurie.reilly <@t> jcu.edu.au Wed Sep 19 03:06:29 2007 From: laurie.reilly <@t> jcu.edu.au (Laurie Reilly) Date: Wed Sep 19 03:06:51 2007 Subject: [Histonet] OT threads In-Reply-To: References: Message-ID: <002f01c7fa93$fbb04c00$5255db89@health.ad.jcu.edu.au> Good for you ,Louise. OT, in moderation, is fine by me. Regards, Laurie. Mr. Laurie REILLY Histopathology School of Veterinary and Biomedical Sciences James Cook University Townsville Qld. 4811 Australia. Phone 07 4781 4468 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise renton Sent: Wednesday, 19 September 2007 5:23 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] OT threads You know a little tolerance goes a long way. In the many years since the inception of Histonet, I can count on the fingers of one hand how many times the threads have gone off course. Whether or not they stay on course is a matter of opinion. Should European, Australians & South Africans all jump up & down when CLIA, CAP or HT accreditation & state licensure is mentioned and discussed at length? This has no bearing on histology in these countries. So come on guys, lighten up & put things into perspective. Remember the adage "All work & no play makes Jack a dull boy" Louise Renton (in asbestos suit)* * do not try this in your lab without prior consultation with safety officers, CAP, JHCO, etc Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From drkwolfe <@t> telus.net Wed Sep 19 04:32:08 2007 From: drkwolfe <@t> telus.net (Joseph Kapler) Date: Wed Sep 19 04:32:15 2007 Subject: [Histonet] OT threads In-Reply-To: <002f01c7fa93$fbb04c00$5255db89@health.ad.jcu.edu.au> References: <002f01c7fa93$fbb04c00$5255db89@health.ad.jcu.edu.au> Message-ID: <000301c7fa9f$f3522720$d9f67560$@net> I'm a Student (Mature Student) taking my courses as I work two jobs (one in Histology), so I find Histonet as one of the best resources for information related to my area of study. But I am also a major NFL fan. I enjoy a discussion from time to time on my favorite Sport, or even some of my favorite music. As stated below, "all work makes joe a dull boy" is not only true, it also leads to burnout and neurosis. Therefore, in MODERATION, a little off topic discussion can go a long way to easing our daily stress. But Moderation is the key. Keep it to a single thread. And remind people its off topic. Maybe that would help... maybe it won't ... but that's my two cents today Joe "DarkWolfe" Kapler -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Reilly Sent: Wednesday, September 19, 2007 2:06 AM To: 'louise renton'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] OT threads Good for you ,Louise. OT, in moderation, is fine by me. Regards, Laurie. Mr. Laurie REILLY Histopathology School of Veterinary and Biomedical Sciences James Cook University Townsville Qld. 4811 Australia. Phone 07 4781 4468 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise renton Sent: Wednesday, 19 September 2007 5:23 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] OT threads You know a little tolerance goes a long way. In the many years since the inception of Histonet, I can count on the fingers of one hand how many times the threads have gone off course. Whether or not they stay on course is a matter of opinion. Should European, Australians & South Africans all jump up & down when CLIA, CAP or HT accreditation & state licensure is mentioned and discussed at length? This has no bearing on histology in these countries. So come on guys, lighten up & put things into perspective. Remember the adage "All work & no play makes Jack a dull boy" Louise Renton (in asbestos suit)* * do not try this in your lab without prior consultation with safety officers, CAP, JHCO, etc Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From karenadams <@t> comcast.net Wed Sep 19 05:35:21 2007 From: karenadams <@t> comcast.net (karenadams@comcast.net) Date: Wed Sep 19 05:35:34 2007 Subject: [Histonet] workstation ergonomics Message-ID: <091920071035.18970.46F0FB690002DE5400004A1A22070206539C030E0B0E020A9D0E05@comcast.net> We are remodeling our existing lab and I would like input into the pros and cons of workstation design, specifically the semi circular cutout vs and L-shaped design in addition to dimensions necessary to accommodate workstation supplies.....any and all input into things that you hate and things you love would be MUCH appreciated!!!! -- Karen Adams Pathology Laboratories West 9303 Park West Blvd Knoxville, TN 37923 (865) 690-2111 FAX (865) 691-1623 From lhadley <@t> iupui.edu Wed Sep 19 06:29:10 2007 From: lhadley <@t> iupui.edu (Baldridge, Lee Ann) Date: Wed Sep 19 06:29:25 2007 Subject: {SPAM?} Re: [Histonet] Football and Flaming In-Reply-To: <508022.93552.qm@web52501.mail.re2.yahoo.com> References: <632276.93450.qm@web53606.mail.re2.yahoo.com> <508022.93552.qm@web52501.mail.re2.yahoo.com> Message-ID: <1E3324ECFB5D5F4DAFEDD17203EEBF2101358F58@iu-mssg-mbx105.ads.iu.edu> Well said Patricia. I totally agree. Oh, Go Colts!!! Lee Ann Baldridge IUSM Indpls.,IN. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patricia Adams Sent: Tuesday, September 18, 2007 4:58 PM To: HistoNet Subject: RE: {SPAM?} Re: [Histonet] Football and Flaming I know that sometimes wading through unwanted histonet "stuff" can be a trial. I feel that way when someone wants to know about some procedure I have never heard of or will never use. I do hit the delete key. Or sometimes take the time to learn something new. I also really enjoy the off topic histo threads, makes me know that all these very smart Techs are also human and would be fun to hang around with. So please keep the humor and wit going! Patrica --- Larry Woody wrote: > Did someone from Detroit mention a party? > Histology people never party, we are always > business and the fact that both words are even > mentioned in the same sentence makes me want to > get back to work! I can't help it, my barcode > is not scanning properly. > > Douglas D Deltour wrote: > Sorry Nancy. > > I understand that football is non-existent in > Detroit. :) > > We will get back to the Histology Stuff. > > Douglas D. Deltour HT(ASCP) > Histology Manager > Professional Pathology Services, PC > One Science Court > Suite 200 > Columbia, SC 29203 > Office (803)252-1913 > Fax (803)254-3262 > Doug@ppspath.com > ***************************************************** > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of > the individual or entity to > which it is addressed and may contain > information that is privileged, > confidential and exempt from disclosure under > applicable law. If the reader > of this message is not the intended recipient, > you are hereby notified that > any dissemination, distribution, or copying of > this communication is > strictly prohibited by law. If you have > received this communication in > error, please notify me immediately. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Nancy Lemke > Sent: Tuesday, September 18, 2007 1:57 PM > To: Thomas Pier; > histonet@lists.utsouthwestern.edu; > sbreeden@nmda.nmsu.edu > Subject: {SPAM?} Re: [Histonet] Football and > Flaming > > Sorry to be a party pooper but is it possible > to eliminate the non histology > threads from the general list and move that > type of communication to direct > emails between the interested parties? It feels > like a low-level hijacking > when Histonet becomes engulfed in one of these > threads. > Thanks > > Nancy Lemke > Research Coordinator > Hermelin Brain Tumor Center > Henry Ford Hospital > Detroit > -----Original message----- > From: "Thomas Pier" tp2@medicine.wisc.edu > Date: Tue, 18 Sep 2007 14:44:03 -0400 > To: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Football and Flaming > > > Sall, > > Ummm, where do I begin. "America's Team" > still can't match the 12 time > > world champ Packers. That's right. 12. 12 is > more than anyone else has. > > Roger Staubach is no Bart Starr and Troy Aikman > is no Brett Favre. I'm > > not even going to start on that crackhead > Michael Irvin. Your boy Tony > > Romo is from Wisconsin too. It would also seem > to me that "your" Braves > > won the 1957 World Series over the New York > Yankees before Atlanta stole > > them from Milwaukee. No flame, just facts, > there you go. > > > > Tom Pier > > Proud Wisconsinite > > > > >>> "Breeden, Sara" 09/18/07 1:03 PM >>> > > I've been quiet lately, mainly because as my > vacation approaches (3 > > days!), all **** is breaking loose (that > happens when one is the Only > > histotech). However, this Football Thing has > gotten my attention. No > > one has said anything about America's Team - > the Dallas Cowboys! Now, > > ya'll just have a big ol' time flaming me > because my skin is so thick > > from years of totally unwarranted abuse by > lesser teams' fans that you > > can't hurt me. Besides, I've given up that > highly overrated physical > > sport for the real sport of BASEBALL - where > stealth and cunning mean so > > much more. Go Braves!! I was further sure of > my Sports Choice last > > year when my friend Linda Blazek and I paid > way too much money for two > > tickets to see the D-Backs in PHX. But we had > great seats! So, give me > > your best shot and I'll try to remember I > asked for it... Vacation > > cures almost everything... > > > > > > > > Sally Breeden, HT(ASCP) > > > > NM Dept. of Agriculture > > > > Veterinary Diagnostic Services > > > > PO Box 4700 > > > > Albuquerque, NM 87106 > > > > 505-841-2576 > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ======================================================================== ==== > == > CONFIDENTIALITY NOTICE: This email contains > information from the sender that > may be CONFIDENTIAL, LEGALLY PRIVILEGED, > PROPRIETARY or otherwise protected > from disclosure. This email is intended for use > only by the person or entity > to whom it is addressed. If you are not the > intended recipient, any use, > disclosure, copying, distribution, printing, or > any action taken in reliance > on the contents of this email, is strictly > prohibited. If you received this > email in error, please contact the sending > party by reply email, delete the > email from your computer system and shred any > paper copies. > > Note to Patients: There are a number of risks > you should consider before > using e-mail to communicate with us. See our > Privacy Policy and Henry Ford > My Health at www.henryford.com for more > detailed information. If you do not > believe that our policy gives you the privacy > and security protection you > need, do not send e-mail or Internet > communications to us. > > ======================================================================== ==== > == > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Tonight's top picks. What will you watch > tonight? Preview the hottest shows on Yahoo! > TV. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ________________________________________________________________________ ____________ Luggage? GPS? Comic books? Check out fitting gifts for grads at Yahoo! Search http://search.yahoo.com/search?fr=oni_on_mail&p=graduation+gifts&cs=bz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rgarhart <@t> system1.net Wed Sep 19 06:59:22 2007 From: rgarhart <@t> system1.net (Robert Garhart) Date: Wed Sep 19 06:59:36 2007 Subject: [Histonet] Need Your Help to Find a Cytology Supervisor In-Reply-To: <000201c77d29$5ef145f0$800aa8c0@domain.local> References: <000201c77d29$5ef145f0$800aa8c0@domain.local> Message-ID: I know this is Histonet but I wanted to see if you could help me. I am looking for the following: Position: Cytology Supervisor with FISH experience. Would be involved in the selection and addition of new installations as a well backed lab continues to grow. Great growth opportunities as this lab continues to expand. Location: NJ Please pass this along to any cytology supervisors you know or point me in the direction of a similar listserve if you know of one for the Cytology side of things. Thanks for your help. Robert Garhart Executive Recruiter System 1 Search 678-342-9029 Office rgarhart@system1.net Website: www.system1.net From Anna.Inman <@t> stmarygj.org Wed Sep 19 07:13:24 2007 From: Anna.Inman <@t> stmarygj.org (Inman, Anna) Date: Wed Sep 19 07:14:28 2007 Subject: [Histonet] Need Your Help to Find a Cytology Supervisor In-Reply-To: Message-ID: <2925AE271EAAD440AF48FCCEB8002D090542F79C@smgmail01.smgj.sclhs.net> We are also looking for a Cytology Supervisor (no FISH experience required) In Grand Junction, Colorado. Great question - Is there a listserv for Cytology? Thank you! Anna Inman SMH Pathology (970)244-7098 Anna.Inman@stmarygj.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Garhart Sent: Wednesday, September 19, 2007 5:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Need Your Help to Find a Cytology Supervisor I know this is Histonet but I wanted to see if you could help me. I am looking for the following: Position: Cytology Supervisor with FISH experience. Would be involved in the selection and addition of new installations as a well backed lab continues to grow. Great growth opportunities as this lab continues to expand. Location: NJ Please pass this along to any cytology supervisors you know or point me in the direction of a similar listserve if you know of one for the Cytology side of things. Thanks for your help. Robert Garhart Executive Recruiter System 1 Search 678-342-9029 Office rgarhart@system1.net Website: www.system1.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From doug <@t> ppspath.com Wed Sep 19 08:28:48 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Wed Sep 19 07:29:22 2007 Subject: [Histonet] What are those things called? Message-ID: I am looking for the "cold bath" or whatever they are called for frozen sectioning. They are the things that you put the 2-methyl butane in to freeze the molds. Any idea on who carries them? Thanks. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. From Terry.Marshall <@t> rothgen.nhs.uk Wed Sep 19 07:50:19 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Wed Sep 19 07:51:13 2007 Subject: [Histonet] Re: Lymph node fixative/enhance solutions Message-ID: <407F05A128805F4C879A33DBA32E618E01895061@TRFT-EX01.xRothGen.nhs.uk> Our associated teaching hospital uses xylene to clear the fat for nodes. Has anybody experience of this? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: 11 September 2007 18:53 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Lymph node fixative/enhance solutions Cindi Robinson, HT (ASCP) in South Dakota asks: >>Our pathologists would like us to start using a solution to help with the dissection and identification of lymph nodes at gross. - We do use Pen-Fix but it doesn't dissolve the fat like the paths would like.<< And Dana Dittus at Polysciences notes: >>Polysciences,Inc manufactures Hartmanns Fixative which turns lymph nodes white. For more info see www.polysciences.com << It's easy and cheap to prepare Davidson's fixative (same as Hartmann's fixative) - mix 3 parts water, 3 parts reagent alcohol, 2 parts strong (37%) formalin, not the buffered kind, and 1 part glacial acetic acid. Color it with eosin if you want to. When Dr. William Hartmann (later the chair of the American Board of Pathology) was at Johns Hopkins in the early 1960's, Dr. Victor McCusick (the medical geneticist, later chair of medicine at Hopkins) asked him to introduce the fixative for Barr bodies (sex chromatin bodies) that Moore and Barr had specified in their original articles on Barr bodies around 1954. Moore and Barr specified "modified Davidson's fixative", which Bill Hartmann introduced. It came into rather widespread use at Hopkins, and Hartmann's name stuck to it. I think the name should not be used, because of possible disastrous confusion with Hartmann's solution, lactated Ringer's solution, introduced into pediatric practice in the 1930's by Dr. Alexis Hartmann Sr. at Washington University in St.Louis. Dr. Alexis Hartmann was one of my clinical teachers, right before he retired around 1964 (sorry about all this name dropping). John Kiernan and I discussed Davidson's fixative in this forum several years ago. He noted that it is not really a rational formula. He hoped to have the time to research the fixative in Davidson's papers, since it seems never to have been published. Getting back to the actual subject, I've used Dissect Aid (Decal Corporation) with good results - got a bottle of it on the shelf right now, in fact. In my brief experience with Penn-Fix, it did not disclose lymph nodes well. Let me support your pathologists very strongly in this endeavor. Just one positive lymph node upstages a colon cancer and makes chemotherapy mandatory. And in colon cancer, those postive lymph nodes can be the size of pinheads. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From koellingr <@t> comcast.net Wed Sep 19 07:54:31 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Wed Sep 19 07:54:46 2007 Subject: [Histonet] OT threads Message-ID: <091920071254.29636.46F11C0700051F30000073C422007621949D09020704040A0105@comcast.net> OT threads do not bother me one way or the other. I just shake my head, ignore and move on to more important things. But I am curious as a biologist, not even counting the talk of CLIA, CAP or HT topics and just counting OT since the inception of the HistoNet regarding guns, Dieties, parties, personal insults, perversions, political incorrectness, recipes, ponds, lakes, scams, vacations, tirades, sexual innuendo, firings, football/soccer, scoldings and multiple topics too difficult to categorize, someone has several hundred fingers, possibly 500, on just one hand? dull Ray Koelling PhenoPath Labs Seattle, WA -------------- Original message -------------- From: "louise renton" > You know a little tolerance goes a long way. > > In the many years since the inception of Histonet, I can count on the > fingers of one hand how many times the threads have gone off course. > Whether or not they stay on course is a matter of opinion. > > Should European, Australians & South Africans all jump up & down when CLIA, > CAP or HT accreditation & state licensure is mentioned and discussed at > length? This has no bearing on histology in these countries. So come on > guys, lighten up & put things into perspective. Remember the adage "All work > & no play makes Jack a dull boy" > > > > Louise Renton (in asbestos suit)* > * do not try this in your lab without prior consultation with safety > officers, CAP, JHCO, etc > > > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > "There are nights when the wolves are silent and only the moon howls". > George Carlin > No trees were killed in the sending of this message. > However, many electrons were terribly inconvenienced. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Wed Sep 19 08:54:47 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Wed Sep 19 07:55:20 2007 Subject: {SPAM?} [Histonet] What are those things called? Message-ID: Never mind I found it. I hate it when I do that. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Wednesday, September 19, 2007 8:29 AM To: histonet@lists.utsouthwestern.edu Subject: {SPAM?} [Histonet] What are those things called? I am looking for the "cold bath" or whatever they are called for frozen sectioning. They are the things that you put the 2-methyl butane in to freeze the molds. Any idea on who carries them? Thanks. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Wed Sep 19 08:16:23 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Sep 19 08:16:37 2007 Subject: [Histonet] Commentary Message-ID: <009f01c7fabf$492ecd50$0202a8c0@yourxhtr8hvc4p> I see by some of the comments that recent topics have upset some people. It is a sad day when we take ourselves so seriously that we can not diverse for a day or two. We work hard day in and day out and with the high quality and time constraints, we are constantly under pressure. Is it so bad to stop and smell the roses once in a while? Ninety-nine per cent of the Histonet does deal with histology. Is it so bad to once in a while to talk about something else such as recipes, football, dresses or perfumes? As a manager, I encourage my techs and students to develop interests outside of histology. See, I didn't take my own advice and became so burnt out that I had to quit my job and take the summer off. This was evident when I had 7 1/2 weeks of vacation on the books. I have learned a lot from this forum and hopefully I have given some instruction back. This includes histology and non-histology topics. Let's lighten up a bit, it's healthy. Joe Nocito BS, PA, HT(ASCP)QIHC San Antonio, TX From jnocito <@t> satx.rr.com Wed Sep 19 08:18:44 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Sep 19 08:18:54 2007 Subject: [Histonet] OT threads References: <002f01c7fa93$fbb04c00$5255db89@health.ad.jcu.edu.au> <000301c7fa9f$f3522720$d9f67560$@net> Message-ID: <00d701c7fabf$9b7bd530$0202a8c0@yourxhtr8hvc4p> hey, I'm not dull JTT ----- Original Message ----- From: "Joseph Kapler" To: Sent: Wednesday, September 19, 2007 4:32 AM Subject: RE: [Histonet] OT threads > I'm a Student (Mature Student) taking my courses as I work two jobs (one > in > Histology), so I find Histonet as one of the best resources for > information > related to my area of study. > > But I am also a major NFL fan. I enjoy a discussion from time to time on > my > favorite Sport, or even some of my favorite music. > > As stated below, "all work makes joe a dull boy" is not only true, it also > leads to burnout and neurosis. Therefore, in MODERATION, a little off > topic > discussion can go a long way to easing our daily stress. But Moderation is > the key. Keep it to a single thread. And remind people its off topic. > > Maybe that would help... maybe it won't ... but that's my two cents today > > Joe "DarkWolfe" Kapler > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie > Reilly > Sent: Wednesday, September 19, 2007 2:06 AM > To: 'louise renton'; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] OT threads > > Good for you ,Louise. OT, in moderation, is fine by me. > > Regards, Laurie. > > Mr. Laurie REILLY > Histopathology > School of Veterinary and Biomedical Sciences > James Cook University > Townsville Qld. 4811 > Australia. > > Phone 07 4781 4468 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise > renton > Sent: Wednesday, 19 September 2007 5:23 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] OT threads > > You know a little tolerance goes a long way. > > In the many years since the inception of Histonet, I can count on the > fingers of one hand how many times the threads have gone off course. > Whether or not they stay on course is a matter of opinion. > > Should European, Australians & South Africans all jump up & down when > CLIA, > CAP or HT accreditation & state licensure is mentioned and discussed at > length? This has no bearing on histology in these countries. So come on > guys, lighten up & put things into perspective. Remember the adage "All > work > & no play makes Jack a dull boy" > > > > Louise Renton (in asbestos suit)* > * do not try this in your lab without prior consultation with safety > officers, CAP, JHCO, etc > > > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > "There are nights when the wolves are silent and only the moon howls". > George Carlin > No trees were killed in the sending of this message. > However, many electrons were terribly inconvenienced. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From slappycraw <@t> yahoo.com Wed Sep 19 08:21:58 2007 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Wed Sep 19 08:22:10 2007 Subject: [Histonet] OT threads In-Reply-To: <091920071254.29636.46F11C0700051F30000073C422007621949D09020704040A0105@comcast.net> Message-ID: <339393.60920.qm@web53608.mail.re2.yahoo.com> I think they are counting hanging chads. koellingr@comcast.net wrote: OT threads do not bother me one way or the other. I just shake my head, ignore and move on to more important things. But I am curious as a biologist, not even counting the talk of CLIA, CAP or HT topics and just counting OT since the inception of the HistoNet regarding guns, Dieties, parties, personal insults, perversions, political incorrectness, recipes, ponds, lakes, scams, vacations, tirades, sexual innuendo, firings, football/soccer, scoldings and multiple topics too difficult to categorize, someone has several hundred fingers, possibly 500, on just one hand? dull Ray Koelling PhenoPath Labs Seattle, WA -------------- Original message -------------- From: "louise renton" > You know a little tolerance goes a long way. > > In the many years since the inception of Histonet, I can count on the > fingers of one hand how many times the threads have gone off course. > Whether or not they stay on course is a matter of opinion. > > Should European, Australians & South Africans all jump up & down when CLIA, > CAP or HT accreditation & state licensure is mentioned and discussed at > length? This has no bearing on histology in these countries. So come on > guys, lighten up & put things into perspective. Remember the adage "All work > & no play makes Jack a dull boy" > > > > Louise Renton (in asbestos suit)* > * do not try this in your lab without prior consultation with safety > officers, CAP, JHCO, etc > > > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > "There are nights when the wolves are silent and only the moon howls". > George Carlin > No trees were killed in the sending of this message. > However, many electrons were terribly inconvenienced. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: mail, news, photos & more. From slappycraw <@t> yahoo.com Wed Sep 19 08:25:44 2007 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Wed Sep 19 08:25:57 2007 Subject: [Histonet] Commentary In-Reply-To: <009f01c7fabf$492ecd50$0202a8c0@yourxhtr8hvc4p> Message-ID: <127664.31138.qm@web53609.mail.re2.yahoo.com> Agreed Joe, it's called work/life balance but when your work is your life like so many people in America, it's impossible to achieve balance. Joe Nocito wrote: I see by some of the comments that recent topics have upset some people. It is a sad day when we take ourselves so seriously that we can not diverse for a day or two. We work hard day in and day out and with the high quality and time constraints, we are constantly under pressure. Is it so bad to stop and smell the roses once in a while? Ninety-nine per cent of the Histonet does deal with histology. Is it so bad to once in a while to talk about something else such as recipes, football, dresses or perfumes? As a manager, I encourage my techs and students to develop interests outside of histology. See, I didn't take my own advice and became so burnt out that I had to quit my job and take the summer off. This was evident when I had 7 1/2 weeks of vacation on the books. I have learned a lot from this forum and hopefully I have given some instruction back. This includes histology and non-histology topics. Let's lighten up a bit, it's healthy. Joe Nocito BS, PA, HT(ASCP)QIHC San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better Heartthrob. Get better relationship answers from someone who knows. Yahoo! Answers - Check it out. From ree3 <@t> leicester.ac.uk Wed Sep 19 08:25:47 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Wed Sep 19 08:26:04 2007 Subject: [Histonet] OTT threads In-Reply-To: <00d701c7fabf$9b7bd530$0202a8c0@yourxhtr8hvc4p> References: <002f01c7fa93$fbb04c00$5255db89@health.ad.jcu.edu.au><000301c7fa9f$f3522720$d9f67560$@net> <00d701c7fabf$9b7bd530$0202a8c0@yourxhtr8hvc4p> Message-ID: Perhaps, just a touch opaque?. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: 19 September 2007 14:19 To: drkwolfe@telus.net; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] OT threads hey, I'm not dull JTT ----- Original Message ----- From: "Joseph Kapler" To: Sent: Wednesday, September 19, 2007 4:32 AM Subject: RE: [Histonet] OT threads > I'm a Student (Mature Student) taking my courses as I work two jobs (one > in > Histology), so I find Histonet as one of the best resources for > information > related to my area of study. > > But I am also a major NFL fan. I enjoy a discussion from time to time on > my > favorite Sport, or even some of my favorite music. > > As stated below, "all work makes joe a dull boy" is not only true, it also > leads to burnout and neurosis. Therefore, in MODERATION, a little off > topic > discussion can go a long way to easing our daily stress. But Moderation is > the key. Keep it to a single thread. And remind people its off topic. > > Maybe that would help... maybe it won't ... but that's my two cents today > > Joe "DarkWolfe" Kapler > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie > Reilly > Sent: Wednesday, September 19, 2007 2:06 AM > To: 'louise renton'; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] OT threads > > Good for you ,Louise. OT, in moderation, is fine by me. > > Regards, Laurie. > > Mr. Laurie REILLY > Histopathology > School of Veterinary and Biomedical Sciences > James Cook University > Townsville Qld. 4811 > Australia. > > Phone 07 4781 4468 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise > renton > Sent: Wednesday, 19 September 2007 5:23 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] OT threads > > You know a little tolerance goes a long way. > > In the many years since the inception of Histonet, I can count on the > fingers of one hand how many times the threads have gone off course. > Whether or not they stay on course is a matter of opinion. > > Should European, Australians & South Africans all jump up & down when > CLIA, > CAP or HT accreditation & state licensure is mentioned and discussed at > length? This has no bearing on histology in these countries. So come on > guys, lighten up & put things into perspective. Remember the adage "All > work > & no play makes Jack a dull boy" > > > > Louise Renton (in asbestos suit)* > * do not try this in your lab without prior consultation with safety > officers, CAP, JHCO, etc > > > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > "There are nights when the wolves are silent and only the moon howls". > George Carlin > No trees were killed in the sending of this message. > However, many electrons were terribly inconvenienced. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jennifer.l.hofecker <@t> Vanderbilt.Edu Wed Sep 19 08:25:53 2007 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Wed Sep 19 08:26:07 2007 Subject: [Histonet] OT threads (Joe's not dull) In-Reply-To: <00d701c7fabf$9b7bd530$0202a8c0@yourxhtr8hvc4p> Message-ID: <898D946569A27444B65667A49C074052F7296A@mailbe06.mc.vanderbilt.edu> I'll second that! Joe and "dull" in the same sentence, that's a first. Oh, man - Now I've jumped into OT land, too! Joe, look what you made me do :) Looking for flame retardant suit... Jennifer -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, September 19, 2007 8:19 AM To: drkwolfe@telus.net; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] OT threads hey, I'm not dull JTT ----- Original Message ----- From: "Joseph Kapler" To: Sent: Wednesday, September 19, 2007 4:32 AM Subject: RE: [Histonet] OT threads > I'm a Student (Mature Student) taking my courses as I work two jobs > (one > in > Histology), so I find Histonet as one of the best resources for > information > related to my area of study. > > But I am also a major NFL fan. I enjoy a discussion from time to time > on > my > favorite Sport, or even some of my favorite music. > > As stated below, "all work makes joe a dull boy" is not only true, it > also leads to burnout and neurosis. Therefore, in MODERATION, a little > off topic discussion can go a long way to easing our daily stress. But > Moderation is the key. Keep it to a single thread. And remind people > its off topic. > > Maybe that would help... maybe it won't ... but that's my two cents > today > > Joe "DarkWolfe" Kapler > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie > Reilly > Sent: Wednesday, September 19, 2007 2:06 AM > To: 'louise renton'; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] OT threads > > Good for you ,Louise. OT, in moderation, is fine by me. > > Regards, Laurie. > > Mr. Laurie REILLY > Histopathology > School of Veterinary and Biomedical Sciences > James Cook University > Townsville Qld. 4811 > Australia. > > Phone 07 4781 4468 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise > renton > Sent: Wednesday, 19 September 2007 5:23 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] OT threads > > You know a little tolerance goes a long way. > > In the many years since the inception of Histonet, I can count on the > fingers of one hand how many times the threads have gone off course. > Whether or not they stay on course is a matter of opinion. > > Should European, Australians & South Africans all jump up & down when > CLIA, > CAP or HT accreditation & state licensure is mentioned and discussed at > length? This has no bearing on histology in these countries. So come on > guys, lighten up & put things into perspective. Remember the adage "All > work > & no play makes Jack a dull boy" > > > > Louise Renton (in asbestos suit)* > * do not try this in your lab without prior consultation with safety > officers, CAP, JHCO, etc > > > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > "There are nights when the wolves are silent and only the moon howls". > George Carlin No trees were killed in the sending of this message. > However, many electrons were terribly inconvenienced. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Wed Sep 19 08:27:31 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Sep 19 08:27:41 2007 Subject: [Histonet] Floor in a pathology laboratory References: <18E3095CC1A30E4684F55D6D757C8D2E51646A@phoex1.peacehealth.org> <413123.40754.qm@web61218.mail.yahoo.com> <6.0.0.22.1.20070918142638.01b34e08@gemini.msu.montana.edu> Message-ID: <011f01c7fac0$d6778390$0202a8c0@yourxhtr8hvc4p> I had some carpets like this and is was a bear to work with in the embedding area. The wheels of the chairs kept causing the carpet to rise up, causing a tripping hazard. Anyone else experience this? By the microtomes, the carpet worked fine. JTT ----- Original Message ----- From: "Gayle Callis" To: "Rene J Buesa" ; Sent: Tuesday, September 18, 2007 3:41 PM Subject: Re: [Histonet] Floor in a pathology laboratory I agree with Rene. We also have commerical rugs (heavy duty, very low carpet, inexpensive, and are the kind found in entry ways of buildings). We place these in front of microtome areas, sinks and processor, chairs roll on them without resistance. They can be vacuumed to pick up paraffin shavings, and when eventually trashed, merely replaced. We had an incident on a hard floor, as paraffin seems to permeate and coat all surfaces. A visiting post doc slipped, and if he hadn't grabbed a door handle, would have cracked back of his skull on our hard floor. Our janitors here would NEVER think to mop a floor, but they do vacuum these little carpets. Perhaps clinical labs have better regulations for cleaning histology lab areas than our research facility ( I work in a double wide trailer house! also called a modular) has available. If you can't use carpets, then consider the sticky peel a away mats under your microtoming areas, this catches trimmings that seem to fly around willy nilly, no matter what one does. These are also used at entry ways of areas where dust, dirt and/or prion associated work is done. Be sure you ask for acid resistant sinks, that are large, deep and enough of them to do the work necessary, staining, grossing areas, etc, etc. A lab with two sinks like ours is NOT a good situation. Also, ask that the the microtoming area has decent bright lighting and NO air vents just about the microtome area. Good luck and congratulations on being able to design a new laboratory. At 02:14 PM 9/18/2007, you wrote: >Chemicals resistant, anti-slip, and ascrubbable to eliminate paraffin. > Ren? J. > >" Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JCollins <@t> palmbeachpath.com Wed Sep 19 08:32:35 2007 From: JCollins <@t> palmbeachpath.com (Judy Collins) Date: Wed Sep 19 08:32:47 2007 Subject: [Histonet] Off Topic Message-ID: >From my perspective, my company pays my salary to do histology and I use the histonet during work hours because it is histology related. My stress relief needs to be on my own time. Therefore, I think the non-histology chatter should be kept to minimum. A couple of comments are one thing, but sometimes it is excessive. Judy Collins Palm Beach Pathology From tkngflght <@t> yahoo.com Wed Sep 19 08:51:30 2007 From: tkngflght <@t> yahoo.com (Cheryl R. Kerry) Date: Wed Sep 19 08:51:29 2007 Subject: [Histonet] workstation ergonomics In-Reply-To: <091920071035.18970.46F0FB690002DE5400004A1A22070206539C030E0B0E020A9D0E05@comcast.net> Message-ID: <005a01c7fac4$2f35af90$6701a8c0@CHERYLSLAPTOP> This isn't exactly what you're seeking but it helped me a LOT when working with ergonomic issues in my labs: Most every state has training available through Dept of Labor and Industry. I took an ergonomics certification course a LONG time ago--it was awesome. The available training is part of the benefits for the fees you pay as an employer and very applicable to what you're trying to accomplish. See if you're allowed to go or if someone in your facility has been-- Cheryl Cheryl R. Kerry, HT(ASCP) Full Staff Inc. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of karenadams@comcast.net Sent: Wednesday, September 19, 2007 5:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] workstation ergonomics We are remodeling our existing lab and I would like input into the pros and cons of workstation design, specifically the semi circular cutout vs and L-shaped design in addition to dimensions necessary to accommodate workstation supplies.....any and all input into things that you hate and things you love would be MUCH appreciated!!!! -- Karen Adams Pathology Laboratories West 9303 Park West Blvd Knoxville, TN 37923 (865) 690-2111 FAX (865) 691-1623 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Laurie.Cote <@t> cytyc.com Wed Sep 19 08:54:50 2007 From: Laurie.Cote <@t> cytyc.com (Cote, Laurie) Date: Wed Sep 19 08:54:54 2007 Subject: [Histonet] RE: Histonet Digest, Vol 46, Issue 36 In-Reply-To: References: Message-ID: <3E76917CB874EF4DB6DF79DD5CEBC1171B343934@nahqmails9.cytyc.com> Folks, I rarely post on Histonet but I read it avidly. The off topic posts CAN be annoying. I've been considering something for awhile and I wonder how you all would feel about this. I belong to a horse board called UDBB. I log in everyday and can read topics that I care about and none that I don't. I'm thinking Histonet ought to go that way as well. I picture several different areas to check into. Something like H & E, routine sectioning, research oriented topics, IHC and off topics. Something like that. I'd be willing to host such a thing on my website www.mohsop.com. But, I wonder if you guys would log into something like that or do you prefer the emails. Laurie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, September 19, 2007 9:47 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 46, Issue 36 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re:HIF1a (wael swelam) 2. RE: {SPAM?} Re: [Histonet] I think I will give it an acronym (Kemlo Rogerson) 3. OT threads (louise renton) 4. RE: OT threads (Laurie Reilly) 5. RE: OT threads (Joseph Kapler) 6. workstation ergonomics (karenadams@comcast.net) 7. RE: {SPAM?} Re: [Histonet] Football and Flaming (Baldridge, Lee Ann) 8. Need Your Help to Find a Cytology Supervisor (Robert Garhart) 9. RE: Need Your Help to Find a Cytology Supervisor (Inman, Anna) 10. What are those things called? (Douglas D Deltour) 11. RE: Re: Lymph node fixative/enhance solutions (Marshall Terry Dr, Consultant Histopathologist) 12. Re: OT threads (koellingr@comcast.net) 13. RE: {SPAM?} [Histonet] What are those things called? (Douglas D Deltour) 14. Commentary (Joe Nocito) 15. Re: OT threads (Joe Nocito) 16. Re: OT threads (Larry Woody) 17. Re: Commentary (Larry Woody) 18. RE: OTT threads (Edwards, R.E.) 19. RE: OT threads (Joe's not dull) (Hofecker, Jennifer L) 20. Re: Floor in a pathology laboratory (Joe Nocito) 21. Off Topic (Judy Collins) ---------------------------------------------------------------------- Message: 1 Date: Wed, 19 Sep 2007 09:48:35 +0300 From: "wael swelam" Subject: Re:[Histonet] HIF1a To: Histonet@lists.utsouthwestern.edu Message-ID: <96566f7e0709182348u3b6db034l71b09e70b1256a3e@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Peace be upon you, I tried mouse monoclonal antibody against HIF-1 a from Chemicon International Inc. (Temecula, CA, USA, 1:100) and It was working nicely, I would recommend you to use Tris-EDTA for retrival. Good luck -- Wael Mohamed Swelam BDS (Egypt), Oral Pathology MSc (Egypt), Oral Pathology PhD (Japan), Ass. Prof. Oral Biomedical Science dept. Division of Oral Pathology, Faculty of Dentistry, King Faisal University, Dammam, Saudi Arabia, P.O: 1982/3441, http://myprofile.cos.com/wmswelam ------------------------------ Message: 2 Date: Wed, 19 Sep 2007 07:53:48 +0100 From: "Kemlo Rogerson" Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym To: "Dolores Townsend" , "Emily Sours" , , "Douglas D Deltour" Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222ED29@wahtntex2.waht.swest.nhs.uk> Content-Type: text/plain; charset="us-ascii" Football in America, you don't play it do you. Don't you play something called Soccer? God only knows what that is? What's that game you play where you get little diddy men and encase them in plastic with big shoulders and they sorta run at each other, then fall down? Bit like Rugby but for Wussies? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; I want to work for a company that contributes to and is part of the community. I want something not just to invest in. I want something to believe in. --Anita Roddick This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation ------------------------------ Message: 3 Date: Wed, 19 Sep 2007 09:22:59 +0200 From: "louise renton" Subject: [Histonet] OT threads To: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 You know a little tolerance goes a long way. In the many years since the inception of Histonet, I can count on the fingers of one hand how many times the threads have gone off course. Whether or not they stay on course is a matter of opinion. Should European, Australians & South Africans all jump up & down when CLIA, CAP or HT accreditation & state licensure is mentioned and discussed at length? This has no bearing on histology in these countries. So come on guys, lighten up & put things into perspective. Remember the adage "All work & no play makes Jack a dull boy" Louise Renton (in asbestos suit)* * do not try this in your lab without prior consultation with safety officers, CAP, JHCO, etc Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ------------------------------ Message: 4 Date: Wed, 19 Sep 2007 18:06:29 +1000 From: Laurie Reilly Subject: RE: [Histonet] OT threads To: "'louise renton'" , Message-ID: <002f01c7fa93$fbb04c00$5255db89@health.ad.jcu.edu.au> Content-Type: text/plain; charset="us-ascii" Good for you ,Louise. OT, in moderation, is fine by me. Regards, Laurie. Mr. Laurie REILLY Histopathology School of Veterinary and Biomedical Sciences James Cook University Townsville Qld. 4811 Australia. Phone 07 4781 4468 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise renton Sent: Wednesday, 19 September 2007 5:23 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] OT threads You know a little tolerance goes a long way. In the many years since the inception of Histonet, I can count on the fingers of one hand how many times the threads have gone off course. Whether or not they stay on course is a matter of opinion. Should European, Australians & South Africans all jump up & down when CLIA, CAP or HT accreditation & state licensure is mentioned and discussed at length? This has no bearing on histology in these countries. So come on guys, lighten up & put things into perspective. Remember the adage "All work & no play makes Jack a dull boy" Louise Renton (in asbestos suit)* * do not try this in your lab without prior consultation with safety officers, CAP, JHCO, etc Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Wed, 19 Sep 2007 03:32:08 -0600 From: "Joseph Kapler" Subject: RE: [Histonet] OT threads To: Message-ID: <000301c7fa9f$f3522720$d9f67560$@net> Content-Type: text/plain; charset="us-ascii" I'm a Student (Mature Student) taking my courses as I work two jobs (one in Histology), so I find Histonet as one of the best resources for information related to my area of study. But I am also a major NFL fan. I enjoy a discussion from time to time on my favorite Sport, or even some of my favorite music. As stated below, "all work makes joe a dull boy" is not only true, it also leads to burnout and neurosis. Therefore, in MODERATION, a little off topic discussion can go a long way to easing our daily stress. But Moderation is the key. Keep it to a single thread. And remind people its off topic. Maybe that would help... maybe it won't ... but that's my two cents today Joe "DarkWolfe" Kapler -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Reilly Sent: Wednesday, September 19, 2007 2:06 AM To: 'louise renton'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] OT threads Good for you ,Louise. OT, in moderation, is fine by me. Regards, Laurie. Mr. Laurie REILLY Histopathology School of Veterinary and Biomedical Sciences James Cook University Townsville Qld. 4811 Australia. Phone 07 4781 4468 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise renton Sent: Wednesday, 19 September 2007 5:23 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] OT threads You know a little tolerance goes a long way. In the many years since the inception of Histonet, I can count on the fingers of one hand how many times the threads have gone off course. Whether or not they stay on course is a matter of opinion. Should European, Australians & South Africans all jump up & down when CLIA, CAP or HT accreditation & state licensure is mentioned and discussed at length? This has no bearing on histology in these countries. So come on guys, lighten up & put things into perspective. Remember the adage "All work & no play makes Jack a dull boy" Louise Renton (in asbestos suit)* * do not try this in your lab without prior consultation with safety officers, CAP, JHCO, etc Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Wed, 19 Sep 2007 10:35:21 +0000 From: karenadams@comcast.net Subject: [Histonet] workstation ergonomics To: histonet@lists.utsouthwestern.edu Message-ID: <091920071035.18970.46F0FB690002DE5400004A1A22070206539C030E0B0E020A9D0E05@comcast.net> Content-Type: text/plain We are remodeling our existing lab and I would like input into the pros and cons of workstation design, specifically the semi circular cutout vs and L-shaped design in addition to dimensions necessary to accommodate workstation supplies.....any and all input into things that you hate and things you love would be MUCH appreciated!!!! -- Karen Adams Pathology Laboratories West 9303 Park West Blvd Knoxville, TN 37923 (865) 690-2111 FAX (865) 691-1623 ------------------------------ Message: 7 Date: Wed, 19 Sep 2007 07:29:10 -0400 From: "Baldridge, Lee Ann" Subject: RE: {SPAM?} Re: [Histonet] Football and Flaming To: "Patricia Adams" , "HistoNet" Message-ID: <1E3324ECFB5D5F4DAFEDD17203EEBF2101358F58@iu-mssg-mbx105.ads.iu.edu> Content-Type: text/plain; charset="us-ascii" Well said Patricia. I totally agree. Oh, Go Colts!!! Lee Ann Baldridge IUSM Indpls.,IN. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patricia Adams Sent: Tuesday, September 18, 2007 4:58 PM To: HistoNet Subject: RE: {SPAM?} Re: [Histonet] Football and Flaming I know that sometimes wading through unwanted histonet "stuff" can be a trial. I feel that way when someone wants to know about some procedure I have never heard of or will never use. I do hit the delete key. Or sometimes take the time to learn something new. I also really enjoy the off topic histo threads, makes me know that all these very smart Techs are also human and would be fun to hang around with. So please keep the humor and wit going! Patrica --- Larry Woody wrote: > Did someone from Detroit mention a party? > Histology people never party, we are always > business and the fact that both words are even > mentioned in the same sentence makes me want to > get back to work! I can't help it, my barcode > is not scanning properly. > > Douglas D Deltour wrote: > Sorry Nancy. > > I understand that football is non-existent in > Detroit. :) > > We will get back to the Histology Stuff. > > Douglas D. Deltour HT(ASCP) > Histology Manager > Professional Pathology Services, PC > One Science Court > Suite 200 > Columbia, SC 29203 > Office (803)252-1913 > Fax (803)254-3262 > Doug@ppspath.com > ***************************************************** > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of > the individual or entity to > which it is addressed and may contain > information that is privileged, > confidential and exempt from disclosure under > applicable law. If the reader > of this message is not the intended recipient, > you are hereby notified that > any dissemination, distribution, or copying of > this communication is > strictly prohibited by law. If you have > received this communication in > error, please notify me immediately. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Nancy Lemke > Sent: Tuesday, September 18, 2007 1:57 PM > To: Thomas Pier; > histonet@lists.utsouthwestern.edu; > sbreeden@nmda.nmsu.edu > Subject: {SPAM?} Re: [Histonet] Football and > Flaming > > Sorry to be a party pooper but is it possible > to eliminate the non histology > threads from the general list and move that > type of communication to direct > emails between the interested parties? It feels > like a low-level hijacking > when Histonet becomes engulfed in one of these > threads. > Thanks > > Nancy Lemke > Research Coordinator > Hermelin Brain Tumor Center > Henry Ford Hospital > Detroit > -----Original message----- > From: "Thomas Pier" tp2@medicine.wisc.edu > Date: Tue, 18 Sep 2007 14:44:03 -0400 > To: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Football and Flaming > > > Sall, > > Ummm, where do I begin. "America's Team" > still can't match the 12 time > > world champ Packers. That's right. 12. 12 is > more than anyone else has. > > Roger Staubach is no Bart Starr and Troy Aikman > is no Brett Favre. I'm > > not even going to start on that crackhead > Michael Irvin. Your boy Tony > > Romo is from Wisconsin too. It would also seem > to me that "your" Braves > > won the 1957 World Series over the New York > Yankees before Atlanta stole > > them from Milwaukee. No flame, just facts, > there you go. > > > > Tom Pier > > Proud Wisconsinite > > > > >>> "Breeden, Sara" 09/18/07 1:03 PM >>> > > I've been quiet lately, mainly because as my > vacation approaches (3 > > days!), all **** is breaking loose (that > happens when one is the Only > > histotech). However, this Football Thing has > gotten my attention. No > > one has said anything about America's Team - > the Dallas Cowboys! Now, > > ya'll just have a big ol' time flaming me > because my skin is so thick > > from years of totally unwarranted abuse by > lesser teams' fans that you > > can't hurt me. Besides, I've given up that > highly overrated physical > > sport for the real sport of BASEBALL - where > stealth and cunning mean so > > much more. Go Braves!! I was further sure of > my Sports Choice last > > year when my friend Linda Blazek and I paid > way too much money for two > > tickets to see the D-Backs in PHX. But we had > great seats! So, give me > > your best shot and I'll try to remember I > asked for it... Vacation > > cures almost everything... > > > > > > > > Sally Breeden, HT(ASCP) > > > > NM Dept. of Agriculture > > > > Veterinary Diagnostic Services > > > > PO Box 4700 > > > > Albuquerque, NM 87106 > > > > 505-841-2576 > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ======================================================================== ==== > == > CONFIDENTIALITY NOTICE: This email contains > information from the sender that > may be CONFIDENTIAL, LEGALLY PRIVILEGED, > PROPRIETARY or otherwise protected > from disclosure. 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If you do not > believe that our policy gives you the privacy > and security protection you > need, do not send e-mail or Internet > communications to us. > > ======================================================================== ==== > == > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Tonight's top picks. What will you watch > tonight? Preview the hottest shows on Yahoo! > TV. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ________________________________________________________________________ ____________ Luggage? GPS? Comic books? Check out fitting gifts for grads at Yahoo! Search http://search.yahoo.com/search?fr=oni_on_mail&p=graduation+gifts&cs=bz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Wed, 19 Sep 2007 04:59:22 -0700 From: "Robert Garhart" Subject: [Histonet] Need Your Help to Find a Cytology Supervisor To: Message-ID: Content-Type: text/plain; charset="us-ascii" I know this is Histonet but I wanted to see if you could help me. I am looking for the following: Position: Cytology Supervisor with FISH experience. Would be involved in the selection and addition of new installations as a well backed lab continues to grow. Great growth opportunities as this lab continues to expand. Location: NJ Please pass this along to any cytology supervisors you know or point me in the direction of a similar listserve if you know of one for the Cytology side of things. Thanks for your help. Robert Garhart Executive Recruiter System 1 Search 678-342-9029 Office rgarhart@system1.net Website: www.system1.net ------------------------------ Message: 9 Date: Wed, 19 Sep 2007 06:13:24 -0600 From: "Inman, Anna" Subject: RE: [Histonet] Need Your Help to Find a Cytology Supervisor To: "Robert Garhart" , Message-ID: <2925AE271EAAD440AF48FCCEB8002D090542F79C@smgmail01.smgj.sclhs.net> Content-Type: text/plain; charset="us-ascii" We are also looking for a Cytology Supervisor (no FISH experience required) In Grand Junction, Colorado. Great question - Is there a listserv for Cytology? Thank you! Anna Inman SMH Pathology (970)244-7098 Anna.Inman@stmarygj.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Garhart Sent: Wednesday, September 19, 2007 5:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Need Your Help to Find a Cytology Supervisor I know this is Histonet but I wanted to see if you could help me. I am looking for the following: Position: Cytology Supervisor with FISH experience. Would be involved in the selection and addition of new installations as a well backed lab continues to grow. Great growth opportunities as this lab continues to expand. Location: NJ Please pass this along to any cytology supervisors you know or point me in the direction of a similar listserve if you know of one for the Cytology side of things. Thanks for your help. Robert Garhart Executive Recruiter System 1 Search 678-342-9029 Office rgarhart@system1.net Website: www.system1.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 10 Date: Wed, 19 Sep 2007 08:28:48 -0500 From: "Douglas D Deltour" Subject: [Histonet] What are those things called? To: Message-ID: Content-Type: text/plain; charset="us-ascii" I am looking for the "cold bath" or whatever they are called for frozen sectioning. They are the things that you put the 2-methyl butane in to freeze the molds. Any idea on who carries them? Thanks. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. ------------------------------ Message: 11 Date: Wed, 19 Sep 2007 13:50:19 +0100 From: "Marshall Terry Dr, Consultant Histopathologist" Subject: RE: [Histonet] Re: Lymph node fixative/enhance solutions To: "Robert Richmond" , Message-ID: <407F05A128805F4C879A33DBA32E618E01895061@TRFT-EX01.xRothGen.nhs.uk> Content-Type: text/plain; charset="us-ascii" Our associated teaching hospital uses xylene to clear the fat for nodes. Has anybody experience of this? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: 11 September 2007 18:53 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Lymph node fixative/enhance solutions Cindi Robinson, HT (ASCP) in South Dakota asks: >>Our pathologists would like us to start using a solution to help with the dissection and identification of lymph nodes at gross. - We do use Pen-Fix but it doesn't dissolve the fat like the paths would like.<< And Dana Dittus at Polysciences notes: >>Polysciences,Inc manufactures Hartmanns Fixative which turns lymph nodes white. For more info see www.polysciences.com << It's easy and cheap to prepare Davidson's fixative (same as Hartmann's fixative) - mix 3 parts water, 3 parts reagent alcohol, 2 parts strong (37%) formalin, not the buffered kind, and 1 part glacial acetic acid. Color it with eosin if you want to. When Dr. William Hartmann (later the chair of the American Board of Pathology) was at Johns Hopkins in the early 1960's, Dr. Victor McCusick (the medical geneticist, later chair of medicine at Hopkins) asked him to introduce the fixative for Barr bodies (sex chromatin bodies) that Moore and Barr had specified in their original articles on Barr bodies around 1954. Moore and Barr specified "modified Davidson's fixative", which Bill Hartmann introduced. It came into rather widespread use at Hopkins, and Hartmann's name stuck to it. I think the name should not be used, because of possible disastrous confusion with Hartmann's solution, lactated Ringer's solution, introduced into pediatric practice in the 1930's by Dr. Alexis Hartmann Sr. at Washington University in St.Louis. Dr. Alexis Hartmann was one of my clinical teachers, right before he retired around 1964 (sorry about all this name dropping). John Kiernan and I discussed Davidson's fixative in this forum several years ago. He noted that it is not really a rational formula. He hoped to have the time to research the fixative in Davidson's papers, since it seems never to have been published. Getting back to the actual subject, I've used Dissect Aid (Decal Corporation) with good results - got a bottle of it on the shelf right now, in fact. In my brief experience with Penn-Fix, it did not disclose lymph nodes well. Let me support your pathologists very strongly in this endeavor. Just one positive lymph node upstages a colon cancer and makes chemotherapy mandatory. And in colon cancer, those postive lymph nodes can be the size of pinheads. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Wed, 19 Sep 2007 12:54:31 +0000 From: koellingr@comcast.net Subject: Re: [Histonet] OT threads To: "louise renton" , Histonet@lists.utsouthwestern.edu Message-ID: <091920071254.29636.46F11C0700051F30000073C422007621949D09020704040A0105@comcast.net> Content-Type: text/plain OT threads do not bother me one way or the other. I just shake my head, ignore and move on to more important things. But I am curious as a biologist, not even counting the talk of CLIA, CAP or HT topics and just counting OT since the inception of the HistoNet regarding guns, Dieties, parties, personal insults, perversions, political incorrectness, recipes, ponds, lakes, scams, vacations, tirades, sexual innuendo, firings, football/soccer, scoldings and multiple topics too difficult to categorize, someone has several hundred fingers, possibly 500, on just one hand? dull Ray Koelling PhenoPath Labs Seattle, WA -------------- Original message -------------- From: "louise renton" > You know a little tolerance goes a long way. > > In the many years since the inception of Histonet, I can count on the > fingers of one hand how many times the threads have gone off course. > Whether or not they stay on course is a matter of opinion. > > Should European, Australians & South Africans all jump up & down when CLIA, > CAP or HT accreditation & state licensure is mentioned and discussed at > length? This has no bearing on histology in these countries. So come on > guys, lighten up & put things into perspective. Remember the adage "All work > & no play makes Jack a dull boy" > > > > Louise Renton (in asbestos suit)* > * do not try this in your lab without prior consultation with safety > officers, CAP, JHCO, etc > > > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > "There are nights when the wolves are silent and only the moon howls". > George Carlin > No trees were killed in the sending of this message. > However, many electrons were terribly inconvenienced. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Wed, 19 Sep 2007 08:54:47 -0500 From: "Douglas D Deltour" Subject: RE: {SPAM?} [Histonet] What are those things called? To: Message-ID: Content-Type: text/plain; charset="us-ascii" Never mind I found it. I hate it when I do that. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Wednesday, September 19, 2007 8:29 AM To: histonet@lists.utsouthwestern.edu Subject: {SPAM?} [Histonet] What are those things called? I am looking for the "cold bath" or whatever they are called for frozen sectioning. They are the things that you put the 2-methyl butane in to freeze the molds. Any idea on who carries them? Thanks. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Wed, 19 Sep 2007 08:16:23 -0500 From: "Joe Nocito" Subject: [Histonet] Commentary To: "histonet" Message-ID: <009f01c7fabf$492ecd50$0202a8c0@yourxhtr8hvc4p> Content-Type: text/plain; charset="iso-8859-1" I see by some of the comments that recent topics have upset some people. It is a sad day when we take ourselves so seriously that we can not diverse for a day or two. We work hard day in and day out and with the high quality and time constraints, we are constantly under pressure. Is it so bad to stop and smell the roses once in a while? Ninety-nine per cent of the Histonet does deal with histology. Is it so bad to once in a while to talk about something else such as recipes, football, dresses or perfumes? As a manager, I encourage my techs and students to develop interests outside of histology. See, I didn't take my own advice and became so burnt out that I had to quit my job and take the summer off. This was evident when I had 7 1/2 weeks of vacation on the books. I have learned a lot from this forum and hopefully I have given some instruction back. This includes histology and non-histology topics. Let's lighten up a bit, it's healthy. Joe Nocito BS, PA, HT(ASCP)QIHC San Antonio, TX ------------------------------ Message: 15 Date: Wed, 19 Sep 2007 08:18:44 -0500 From: "Joe Nocito" Subject: Re: [Histonet] OT threads To: , Message-ID: <00d701c7fabf$9b7bd530$0202a8c0@yourxhtr8hvc4p> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original hey, I'm not dull JTT ----- Original Message ----- From: "Joseph Kapler" To: Sent: Wednesday, September 19, 2007 4:32 AM Subject: RE: [Histonet] OT threads > I'm a Student (Mature Student) taking my courses as I work two jobs (one > in > Histology), so I find Histonet as one of the best resources for > information > related to my area of study. > > But I am also a major NFL fan. I enjoy a discussion from time to time on > my > favorite Sport, or even some of my favorite music. > > As stated below, "all work makes joe a dull boy" is not only true, it also > leads to burnout and neurosis. Therefore, in MODERATION, a little off > topic > discussion can go a long way to easing our daily stress. But Moderation is > the key. Keep it to a single thread. And remind people its off topic. > > Maybe that would help... maybe it won't ... but that's my two cents today > > Joe "DarkWolfe" Kapler > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie > Reilly > Sent: Wednesday, September 19, 2007 2:06 AM > To: 'louise renton'; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] OT threads > > Good for you ,Louise. OT, in moderation, is fine by me. > > Regards, Laurie. > > Mr. Laurie REILLY > Histopathology > School of Veterinary and Biomedical Sciences > James Cook University > Townsville Qld. 4811 > Australia. > > Phone 07 4781 4468 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise > renton > Sent: Wednesday, 19 September 2007 5:23 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] OT threads > > You know a little tolerance goes a long way. > > In the many years since the inception of Histonet, I can count on the > fingers of one hand how many times the threads have gone off course. > Whether or not they stay on course is a matter of opinion. > > Should European, Australians & South Africans all jump up & down when > CLIA, > CAP or HT accreditation & state licensure is mentioned and discussed at > length? This has no bearing on histology in these countries. So come on > guys, lighten up & put things into perspective. Remember the adage "All > work > & no play makes Jack a dull boy" > > > > Louise Renton (in asbestos suit)* > * do not try this in your lab without prior consultation with safety > officers, CAP, JHCO, etc > > > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > "There are nights when the wolves are silent and only the moon howls". > George Carlin > No trees were killed in the sending of this message. > However, many electrons were terribly inconvenienced. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Wed, 19 Sep 2007 06:21:58 -0700 (PDT) From: Larry Woody Subject: Re: [Histonet] OT threads To: koellingr@comcast.net, louise renton , Histonet@lists.utsouthwestern.edu Message-ID: <339393.60920.qm@web53608.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I think they are counting hanging chads. koellingr@comcast.net wrote: OT threads do not bother me one way or the other. I just shake my head, ignore and move on to more important things. But I am curious as a biologist, not even counting the talk of CLIA, CAP or HT topics and just counting OT since the inception of the HistoNet regarding guns, Dieties, parties, personal insults, perversions, political incorrectness, recipes, ponds, lakes, scams, vacations, tirades, sexual innuendo, firings, football/soccer, scoldings and multiple topics too difficult to categorize, someone has several hundred fingers, possibly 500, on just one hand? dull Ray Koelling PhenoPath Labs Seattle, WA -------------- Original message -------------- From: "louise renton" > You know a little tolerance goes a long way. > > In the many years since the inception of Histonet, I can count on the > fingers of one hand how many times the threads have gone off course. > Whether or not they stay on course is a matter of opinion. > > Should European, Australians & South Africans all jump up & down when CLIA, > CAP or HT accreditation & state licensure is mentioned and discussed at > length? This has no bearing on histology in these countries. So come on > guys, lighten up & put things into perspective. Remember the adage "All work > & no play makes Jack a dull boy" > > > > Louise Renton (in asbestos suit)* > * do not try this in your lab without prior consultation with safety > officers, CAP, JHCO, etc > > > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > "There are nights when the wolves are silent and only the moon howls". > George Carlin > No trees were killed in the sending of this message. > However, many electrons were terribly inconvenienced. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: mail, news, photos & more. ------------------------------ Message: 17 Date: Wed, 19 Sep 2007 06:25:44 -0700 (PDT) From: Larry Woody Subject: Re: [Histonet] Commentary To: Joe Nocito , histonet Message-ID: <127664.31138.qm@web53609.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Agreed Joe, it's called work/life balance but when your work is your life like so many people in America, it's impossible to achieve balance. Joe Nocito wrote: I see by some of the comments that recent topics have upset some people. It is a sad day when we take ourselves so seriously that we can not diverse for a day or two. We work hard day in and day out and with the high quality and time constraints, we are constantly under pressure. Is it so bad to stop and smell the roses once in a while? Ninety-nine per cent of the Histonet does deal with histology. Is it so bad to once in a while to talk about something else such as recipes, football, dresses or perfumes? As a manager, I encourage my techs and students to develop interests outside of histology. See, I didn't take my own advice and became so burnt out that I had to quit my job and take the summer off. This was evident when I had 7 1/2 weeks of vacation on the books. I have learned a lot from this forum and hopefully I have given some instruction back. This includes histology and non-histology topics. Let's lighten up a bit, it's healthy. Joe Nocito BS, PA, HT(ASCP)QIHC San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better Heartthrob. Get better relationship answers from someone who knows. Yahoo! Answers - Check it out. ------------------------------ Message: 18 Date: Wed, 19 Sep 2007 14:25:47 +0100 From: "Edwards, R.E." Subject: RE: [Histonet] OTT threads To: "Joe Nocito" , , Message-ID: Content-Type: text/plain; charset="US-ASCII" Perhaps, just a touch opaque?. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: 19 September 2007 14:19 To: drkwolfe@telus.net; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] OT threads hey, I'm not dull JTT ----- Original Message ----- From: "Joseph Kapler" To: Sent: Wednesday, September 19, 2007 4:32 AM Subject: RE: [Histonet] OT threads > I'm a Student (Mature Student) taking my courses as I work two jobs (one > in > Histology), so I find Histonet as one of the best resources for > information > related to my area of study. > > But I am also a major NFL fan. I enjoy a discussion from time to time on > my > favorite Sport, or even some of my favorite music. > > As stated below, "all work makes joe a dull boy" is not only true, it also > leads to burnout and neurosis. Therefore, in MODERATION, a little off > topic > discussion can go a long way to easing our daily stress. But Moderation is > the key. Keep it to a single thread. And remind people its off topic. > > Maybe that would help... maybe it won't ... but that's my two cents today > > Joe "DarkWolfe" Kapler > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie > Reilly > Sent: Wednesday, September 19, 2007 2:06 AM > To: 'louise renton'; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] OT threads > > Good for you ,Louise. OT, in moderation, is fine by me. > > Regards, Laurie. > > Mr. Laurie REILLY > Histopathology > School of Veterinary and Biomedical Sciences > James Cook University > Townsville Qld. 4811 > Australia. > > Phone 07 4781 4468 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise > renton > Sent: Wednesday, 19 September 2007 5:23 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] OT threads > > You know a little tolerance goes a long way. > > In the many years since the inception of Histonet, I can count on the > fingers of one hand how many times the threads have gone off course. > Whether or not they stay on course is a matter of opinion. > > Should European, Australians & South Africans all jump up & down when > CLIA, > CAP or HT accreditation & state licensure is mentioned and discussed at > length? This has no bearing on histology in these countries. So come on > guys, lighten up & put things into perspective. Remember the adage "All > work > & no play makes Jack a dull boy" > > > > Louise Renton (in asbestos suit)* > * do not try this in your lab without prior consultation with safety > officers, CAP, JHCO, etc > > > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > "There are nights when the wolves are silent and only the moon howls". > George Carlin > No trees were killed in the sending of this message. > However, many electrons were terribly inconvenienced. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Wed, 19 Sep 2007 08:25:53 -0500 From: "Hofecker, Jennifer L" Subject: RE: [Histonet] OT threads (Joe's not dull) To: "Joe Nocito" , Message-ID: <898D946569A27444B65667A49C074052F7296A@mailbe06.mc.vanderbilt.edu> Content-Type: text/plain; charset="us-ascii" I'll second that! Joe and "dull" in the same sentence, that's a first. Oh, man - Now I've jumped into OT land, too! Joe, look what you made me do :) Looking for flame retardant suit... Jennifer -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, September 19, 2007 8:19 AM To: drkwolfe@telus.net; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] OT threads hey, I'm not dull JTT ----- Original Message ----- From: "Joseph Kapler" To: Sent: Wednesday, September 19, 2007 4:32 AM Subject: RE: [Histonet] OT threads > I'm a Student (Mature Student) taking my courses as I work two jobs > (one > in > Histology), so I find Histonet as one of the best resources for > information > related to my area of study. > > But I am also a major NFL fan. I enjoy a discussion from time to time > on > my > favorite Sport, or even some of my favorite music. > > As stated below, "all work makes joe a dull boy" is not only true, it > also leads to burnout and neurosis. Therefore, in MODERATION, a little > off topic discussion can go a long way to easing our daily stress. But > Moderation is the key. Keep it to a single thread. And remind people > its off topic. > > Maybe that would help... maybe it won't ... but that's my two cents > today > > Joe "DarkWolfe" Kapler > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie > Reilly > Sent: Wednesday, September 19, 2007 2:06 AM > To: 'louise renton'; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] OT threads > > Good for you ,Louise. OT, in moderation, is fine by me. > > Regards, Laurie. > > Mr. Laurie REILLY > Histopathology > School of Veterinary and Biomedical Sciences > James Cook University > Townsville Qld. 4811 > Australia. > > Phone 07 4781 4468 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise > renton > Sent: Wednesday, 19 September 2007 5:23 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] OT threads > > You know a little tolerance goes a long way. > > In the many years since the inception of Histonet, I can count on the > fingers of one hand how many times the threads have gone off course. > Whether or not they stay on course is a matter of opinion. > > Should European, Australians & South Africans all jump up & down when > CLIA, > CAP or HT accreditation & state licensure is mentioned and discussed at > length? This has no bearing on histology in these countries. So come on > guys, lighten up & put things into perspective. Remember the adage "All > work > & no play makes Jack a dull boy" > > > > Louise Renton (in asbestos suit)* > * do not try this in your lab without prior consultation with safety > officers, CAP, JHCO, etc > > > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > "There are nights when the wolves are silent and only the moon howls". > George Carlin No trees were killed in the sending of this message. > However, many electrons were terribly inconvenienced. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Wed, 19 Sep 2007 08:27:31 -0500 From: "Joe Nocito" Subject: Re: [Histonet] Floor in a pathology laboratory To: "Gayle Callis" , "Rene J Buesa" , Message-ID: <011f01c7fac0$d6778390$0202a8c0@yourxhtr8hvc4p> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=response I had some carpets like this and is was a bear to work with in the embedding area. The wheels of the chairs kept causing the carpet to rise up, causing a tripping hazard. Anyone else experience this? By the microtomes, the carpet worked fine. JTT ----- Original Message ----- From: "Gayle Callis" To: "Rene J Buesa" ; Sent: Tuesday, September 18, 2007 3:41 PM Subject: Re: [Histonet] Floor in a pathology laboratory I agree with Rene. We also have commerical rugs (heavy duty, very low carpet, inexpensive, and are the kind found in entry ways of buildings). We place these in front of microtome areas, sinks and processor, chairs roll on them without resistance. They can be vacuumed to pick up paraffin shavings, and when eventually trashed, merely replaced. We had an incident on a hard floor, as paraffin seems to permeate and coat all surfaces. A visiting post doc slipped, and if he hadn't grabbed a door handle, would have cracked back of his skull on our hard floor. Our janitors here would NEVER think to mop a floor, but they do vacuum these little carpets. Perhaps clinical labs have better regulations for cleaning histology lab areas than our research facility ( I work in a double wide trailer house! also called a modular) has available. If you can't use carpets, then consider the sticky peel a away mats under your microtoming areas, this catches trimmings that seem to fly around willy nilly, no matter what one does. These are also used at entry ways of areas where dust, dirt and/or prion associated work is done. Be sure you ask for acid resistant sinks, that are large, deep and enough of them to do the work necessary, staining, grossing areas, etc, etc. A lab with two sinks like ours is NOT a good situation. Also, ask that the the microtoming area has decent bright lighting and NO air vents just about the microtome area. Good luck and congratulations on being able to design a new laboratory. At 02:14 PM 9/18/2007, you wrote: >Chemicals resistant, anti-slip, and ascrubbable to eliminate paraffin. > Ren? J. > >" Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 21 Date: Wed, 19 Sep 2007 09:32:35 -0400 From: "Judy Collins" Subject: [Histonet] Off Topic To: Message-ID: Content-Type: text/plain; charset="us-ascii" >From my perspective, my company pays my salary to do histology and I use the histonet during work hours because it is histology related. My stress relief needs to be on my own time. Therefore, I think the non-histology chatter should be kept to minimum. A couple of comments are one thing, but sometimes it is excessive. Judy Collins Palm Beach Pathology ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 46, Issue 36 **************************************** From cmiller <@t> physlab.com Wed Sep 19 08:58:14 2007 From: cmiller <@t> physlab.com (Cheri Miller) Date: Wed Sep 19 08:58:31 2007 Subject: [Histonet] Last chance! Histology Consumables Product Survey($50Honorarium opportunity) In-Reply-To: <407F05A128805F4C879A33DBA32E618E0189505F@TRFT-EX01.xRothGen.nhs.uk> References: <407F05A128805F4C879A33DBA32E618E0189505F@TRFT-EX01.xRothGen.nhs.uk> Message-ID: <005c01c7fac5$211cdef0$3402a8c0@plab.local> Is this spam?? Or legit Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Tuesday, September 18, 2007 10:38 AM To: Adam Goldstein; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Last chance! Histology Consumables Product Survey($50Honorarium opportunity) Before the Europeans spend time on this, be aware that, from the website of American Express:- "The Gift Card can be used at over a million businesses in the United States where American Express Cards are accepted." Pity you have to go to the US to cash in on it. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adam Goldstein Sent: 18 September 2007 16:09 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Last chance! Histology Consumables Product Survey ($50Honorarium opportunity) Dear Histonetters, This is your last opportunity to participate in a survey that will help shape the future of histology products and earn $50 at the same time! Please respond quickly, as we are only administering the survey for a few more days. If you have already responded, please do not do so again. Our firm, Kaiser Associates, is conducting research on the products and processes for the histologic technique of primary (H&E) staining and would like your input. By participating in this survey, you will be influencing potential upcoming changes to the market for primary (H&E) staining products. The completion of this survey should only take 20-30 minutes, and in return for your participation you will receive an honorarium of US $50 in the form of an American Express gift card. If you would like to participate in this survey please reply to AGoldstein@KaiserAssociates.com with: 1. Your job title; 2. Your department; and 3. Your company/hospital name and location Upon receipt of the above information, we will send you the web link to the survey. Please note that we are primarily interested in respondents from the US or Europe. Thanks! Sincerely, Kaiser Associates, Inc. Research Team About Kaiser Associates, Inc: Kaiser Associates is a research-based international consulting firm dedicated to helping leading global corporations develop effective strategies to drive continued operating performance. For more information please go to www.KaiserAssociates.com . _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From hymclab <@t> hyhc.com Wed Sep 19 09:01:14 2007 From: hymclab <@t> hyhc.com (hymclab) Date: Wed Sep 19 08:59:03 2007 Subject: {SPAM?} Re: [Histonet] Football and Flaming Message-ID: Nicely said!!!! Dawn -----Original Message----- From: Patricia Adams [mailto:alaskagirl1950@yahoo.com] Sent: Tuesday, September 18, 2007 3:58 PM To: HistoNet Subject: RE: {SPAM?} Re: [Histonet] Football and Flaming I know that sometimes wading through unwanted histonet "stuff" can be a trial. I feel that way when someone wants to know about some procedure I have never heard of or will never use. I do hit the delete key. Or sometimes take the time to learn something new. I also really enjoy the off topic histo threads, makes me know that all these very smart Techs are also human and would be fun to hang around with. So please keep the humor and wit going! Patrica --- Larry Woody wrote: > Did someone from Detroit mention a party? > Histology people never party, we are always business and the fact that > both words are even mentioned in the same sentence makes me want to > get back to work! I can't help it, my barcode is not scanning > properly. > > Douglas D Deltour wrote: > Sorry Nancy. > > I understand that football is non-existent in Detroit. :) > > We will get back to the Histology Stuff. > > Douglas D. Deltour HT(ASCP) > Histology Manager > Professional Pathology Services, PC > One Science Court > Suite 200 > Columbia, SC 29203 > Office (803)252-1913 > Fax (803)254-3262 > Doug@ppspath.com > ***************************************************** > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of the individual or entity > to which it is addressed and may contain information that is > privileged, confidential and exempt from disclosure under applicable > law. If the reader of this message is not the intended recipient, you > are hereby notified that any dissemination, distribution, or copying > of this communication is strictly prohibited by law. If you have > received this communication in error, please notify me immediately. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Nancy Lemke > Sent: Tuesday, September 18, 2007 1:57 PM > To: Thomas Pier; > histonet@lists.utsouthwestern.edu; > sbreeden@nmda.nmsu.edu > Subject: {SPAM?} Re: [Histonet] Football and Flaming > > Sorry to be a party pooper but is it possible to eliminate the non > histology threads from the general list and move that type of > communication to direct emails between the interested parties? It > feels like a low-level hijacking when Histonet becomes engulfed in one > of these threads. > Thanks > > Nancy Lemke > Research Coordinator > Hermelin Brain Tumor Center > Henry Ford Hospital > Detroit > -----Original message----- > From: "Thomas Pier" tp2@medicine.wisc.edu > Date: Tue, 18 Sep 2007 14:44:03 -0400 > To: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Football and Flaming > > > Sall, > > Ummm, where do I begin. "America's Team" > still can't match the 12 time > > world champ Packers. That's right. 12. 12 is more than anyone else > has. > Roger Staubach is no Bart Starr and Troy Aikman is no Brett > Favre. I'm > not even going to start on that crackhead Michael Irvin. > Your boy Tony > Romo is from Wisconsin too. It would also seem to me > that "your" Braves > won the 1957 World Series over the New York > Yankees before Atlanta stole > them from Milwaukee. No flame, just > facts, there you go. > > > > Tom Pier > > Proud Wisconsinite > > > > >>> "Breeden, Sara" 09/18/07 1:03 PM >>> > > I've been quiet lately, mainly because as my > vacation approaches (3 > > days!), all **** is breaking loose (that > happens when one is the Only > > histotech). However, this Football Thing has > gotten my attention. No > > one has said anything about America's Team - > the Dallas Cowboys! Now, > > ya'll just have a big ol' time flaming me > because my skin is so thick > > from years of totally unwarranted abuse by > lesser teams' fans that you > > can't hurt me. Besides, I've given up that > highly overrated physical > > sport for the real sport of BASEBALL - where > stealth and cunning mean so > > much more. Go Braves!! I was further sure of > my Sports Choice last > > year when my friend Linda Blazek and I paid > way too much money for two > > tickets to see the D-Backs in PHX. But we had > great seats! So, give me > > your best shot and I'll try to remember I > asked for it... Vacation > > cures almost everything... > > > > > > > > Sally Breeden, HT(ASCP) > > > > NM Dept. of Agriculture > > > > Veterinary Diagnostic Services > > > > PO Box 4700 > > > > Albuquerque, NM 87106 > > > > 505-841-2576 > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ============================================================================ > == > CONFIDENTIALITY NOTICE: This email contains information from the > sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or > otherwise protected from disclosure. This email is intended for use > only by the person or entity to whom it is addressed. If you are not > the intended recipient, any use, disclosure, copying, distribution, > printing, or any action taken in reliance on the contents of this > email, is strictly prohibited. If you received this email in error, > please contact the sending party by reply email, delete the email from > your computer system and shred any paper copies. > > Note to Patients: There are a number of risks you should consider > before using e-mail to communicate with us. See our Privacy Policy and > Henry Ford My Health at www.henryford.com for more detailed > information. If you do not believe that our policy gives you the > privacy and security protection you need, do not send e-mail or > Internet communications to us. > > ============================================================================ > == > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Tonight's top picks. What will you watch tonight? Preview the hottest > shows on Yahoo! > TV. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ____________________________________________________________________________ ________ Luggage? GPS? Comic books? Check out fitting gifts for grads at Yahoo! Search http://search.yahoo.com/search?fr=oni_on_mail&p=graduation+gifts&cs=bz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic message and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. Dissemination, forwarding, printing or copying of this message/documents without the consent of the sender is prohibited. From b-frederick <@t> northwestern.edu Wed Sep 19 09:08:40 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Sep 19 09:08:44 2007 Subject: [Histonet] OT threads In-Reply-To: <00d701c7fabf$9b7bd530$0202a8c0@yourxhtr8hvc4p> Message-ID: <000501c7fac6$974700f0$d00f7ca5@lurie.northwestern.edu> And to think my younger coworkers think two of us are nuts when we HAVE to sing Paradise by the Dashboard Light every time it's on the radio!!! We blame it on the fumes. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, September 19, 2007 8:19 AM To: drkwolfe@telus.net; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] OT threads hey, I'm not dull JTT ----- Original Message ----- From: "Joseph Kapler" To: Sent: Wednesday, September 19, 2007 4:32 AM Subject: RE: [Histonet] OT threads > I'm a Student (Mature Student) taking my courses as I work two jobs (one > in > Histology), so I find Histonet as one of the best resources for > information > related to my area of study. > > But I am also a major NFL fan. I enjoy a discussion from time to time on > my > favorite Sport, or even some of my favorite music. > > As stated below, "all work makes joe a dull boy" is not only true, it also > leads to burnout and neurosis. Therefore, in MODERATION, a little off > topic > discussion can go a long way to easing our daily stress. But Moderation is > the key. Keep it to a single thread. And remind people its off topic. > > Maybe that would help... maybe it won't ... but that's my two cents today > > Joe "DarkWolfe" Kapler > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie > Reilly > Sent: Wednesday, September 19, 2007 2:06 AM > To: 'louise renton'; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] OT threads > > Good for you ,Louise. OT, in moderation, is fine by me. > > Regards, Laurie. > > Mr. Laurie REILLY > Histopathology > School of Veterinary and Biomedical Sciences > James Cook University > Townsville Qld. 4811 > Australia. > > Phone 07 4781 4468 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise > renton > Sent: Wednesday, 19 September 2007 5:23 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] OT threads > > You know a little tolerance goes a long way. > > In the many years since the inception of Histonet, I can count on the > fingers of one hand how many times the threads have gone off course. > Whether or not they stay on course is a matter of opinion. > > Should European, Australians & South Africans all jump up & down when > CLIA, > CAP or HT accreditation & state licensure is mentioned and discussed at > length? This has no bearing on histology in these countries. So come on > guys, lighten up & put things into perspective. Remember the adage "All > work > & no play makes Jack a dull boy" > > > > Louise Renton (in asbestos suit)* > * do not try this in your lab without prior consultation with safety > officers, CAP, JHCO, etc > > > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > "There are nights when the wolves are silent and only the moon howls". > George Carlin > No trees were killed in the sending of this message. > However, many electrons were terribly inconvenienced. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TownsendD <@t> childrensdayton.org Wed Sep 19 09:11:34 2007 From: TownsendD <@t> childrensdayton.org (Dolores Townsend) Date: Wed Sep 19 09:11:59 2007 Subject: [Histonet] Commentary Message-ID: Dresses, Joe? This is a new side of you... Dolores From oshel1pe <@t> cmich.edu Wed Sep 19 09:12:15 2007 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Wed Sep 19 09:12:33 2007 Subject: [Histonet] Floor in a pathology laboratory In-Reply-To: <011f01c7fac0$d6778390$0202a8c0@yourxhtr8hvc4p> References: <18E3095CC1A30E4684F55D6D757C8D2E51646A@phoex1.peacehealth.org> <413123.40754.qm@web61218.mail.yahoo.com> <6.0.0.22.1.20070918142638.01b34e08@gemini.msu.montana.edu> <011f01c7fac0$d6778390$0202a8c0@yourxhtr8hvc4p> Message-ID: Anyone try carpet tape on the bottom? Basically wide, double-sticky tape on steroids, usually used to stick carpet to carpet pad, but it would work to stick carpet to floor. Phil My OT contribution, for Kemlo Rogerson: we may not know football, but at least it's not cricket. >I had some carpets like this and is was a bear >to work with in the embedding area. The wheels >of the chairs kept causing the carpet to rise >up, causing a tripping hazard. Anyone else >experience this? By the microtomes, the carpet >worked fine. > >JTT >----- Original Message ----- From: "Gayle Callis" >To: "Rene J Buesa" ; >Sent: Tuesday, September 18, 2007 3:41 PM >Subject: Re: [Histonet] Floor in a pathology laboratory > > >I agree with Rene. We also have commerical rugs (heavy duty, very low >carpet, inexpensive, and are the kind found in entry ways of >buildings). We place these in front of microtome areas, sinks and >processor, chairs roll on them without resistance. They can be vacuumed >to pick up paraffin shavings, and when eventually trashed, merely >replaced. We had an incident on a hard floor, as paraffin seems to >permeate and coat all surfaces. A visiting post doc slipped, and if he >hadn't grabbed a door handle, would have cracked back of his skull on our >hard floor. Our janitors here would NEVER think to mop a floor, but they >do vacuum these little carpets. Perhaps clinical labs have better >regulations for cleaning histology lab areas than our research facility ( I >work in a double wide trailer house! also called a modular) has available. > >If you can't use carpets, then consider the sticky peel a away mats under >your microtoming areas, this catches trimmings that seem to fly around >willy nilly, no matter what one does. These are also used at entry ways of >areas where dust, dirt and/or prion associated work is done. > >Be sure you ask for acid resistant sinks, that are large, deep and enough >of them to do the work necessary, staining, grossing areas, etc, etc. A >lab with two sinks like ours is NOT a good situation. Also, ask that the >the microtoming area has decent bright lighting and NO air vents just about >the microtome area. > >Good luck and congratulations on being able to design a new laboratory. > >At 02:14 PM 9/18/2007, you wrote: >>Chemicals resistant, anti-slip, and ascrubbable to eliminate paraffin. >> Ren? J. >> >>" > >Gayle Callis >MT,HT,HTL(ASCP) >Research Histopathology Supervisor >Veterinary Molecular Biology >Montana State University - Bozeman >PO Box 173610 >Bozeman MT 59717-3610 -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 From alaskagirl1950 <@t> yahoo.com Wed Sep 19 09:13:54 2007 From: alaskagirl1950 <@t> yahoo.com (Patricia Adams) Date: Wed Sep 19 09:14:07 2007 Subject: [Histonet] Off Topic In-Reply-To: Message-ID: <784.8439.qm@web52508.mail.re2.yahoo.com> Great to be loyal to your employers, but I have worked for some who did not even wish to see the Histo-Techs laughing and having fun in the department. We had to go around with sour looks, guess that showed how we were working hard and making money for them. What they did not know is that we worked better with a smile on our faces. Being serious all the time does not mean you are doing a good job. And also does not bring about good team work. Patricia --- Judy Collins wrote: > >From my perspective, my company pays my salary > to do histology and I use > the histonet during work hours because it is > histology related. My > stress relief needs to be on my own time. > Therefore, I think the > non-histology chatter should be kept to > minimum. A couple of comments > are one thing, but sometimes it is excessive. > > Judy Collins > Palm Beach Pathology > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D ____________________________________________________________________________________ Tonight's top picks. What will you watch tonight? Preview the hottest shows on Yahoo! TV. http://tv.yahoo.com/ From JWEEMS <@t> sjha.org Wed Sep 19 09:23:15 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Sep 19 09:23:46 2007 Subject: [Histonet] RE: OT posts In-Reply-To: <60A63AF3E687254496C3F649564980FA26C56077@sjhaexc02.sjha.org> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F3BE1@sjhaexc02.sjha.org> I prefer emails and enjoy the banter of any kind. We decided a few years ago, that if something was off topic, we would put OT in the subject - which was done in this instance - and of course football in the subject would most likely be off topic :>). If I don't have time, I delete, or I have even put them in a hold folder, just to take a look at when I get time. I enjoy the Friday funnies - any time one can laugh, it takes wrinkles off one's soul.... My 2 cents... Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital of Atlanta 404-851-7376 404-851-7831 - Fax Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From jqb7 <@t> CDC.GOV Wed Sep 19 09:27:42 2007 From: jqb7 <@t> CDC.GOV (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed Sep 19 09:28:07 2007 Subject: [Histonet] RE: OT posts In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F3BE1@sjhaexc02.sjha.org> References: <60A63AF3E687254496C3F649564980FA26C56077@sjhaexc02.sjha.org> <1CD6831EB9B26D45B0A3EAA79F7EBD32048F3BE1@sjhaexc02.sjha.org> Message-ID: <34BB307EFC9A65429BBB49E330675F7202BDC092@LTA3VS003.ees.hhs.gov> I agree 100%! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, September 19, 2007 10:23 AM To: Cote, Laurie; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: OT posts I prefer emails and enjoy the banter of any kind. We decided a few years ago, that if something was off topic, we would put OT in the subject - which was done in this instance - and of course football in the subject would most likely be off topic :>). If I don't have time, I delete, or I have even put them in a hold folder, just to take a look at when I get time. I enjoy the Friday funnies - any time one can laugh, it takes wrinkles off one's soul.... My 2 cents... Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital of Atlanta 404-851-7376 404-851-7831 - Fax Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From mhorne <@t> upei.ca Wed Sep 19 10:04:16 2007 From: mhorne <@t> upei.ca (Margaret Horne) Date: Wed Sep 19 09:42:57 2007 Subject: [Histonet] OT threads In-Reply-To: <091920071254.29636.46F11C0700051F30000073C422007621949D09020704040A0105@comcast.net> Message-ID: <46F1022E.25854.DA3A3@localhost> May I suggest prefacing the subject title with " OT " ? Then a reciever can just hit the " subject" tab at the top of the mailing list, which sorts the messages alphabetically by subject. It is a work of seconds to highlight and delete the whole block of OT messages. Alternatively, one can put on a mail filter that picks up OT as a " word" and deletes these messages so that you never see them at all. I personally really appreciate the OT messages and as I read my Histonet during my coffee break , I don't feel guilty about use of work time. Then at lunchtime I get together with a few friends and practice karate ; also a known stress reliever ;-) Margaret Margaret Horne , Histology Teaching Assistant, Dept. of B.SC., Atlantic Veterinary College, U.P.E.I., 550 University Ave., Charlottetown, P.E.I., C1A 4P3 Canada From donna <@t> milestonemed.com Tue Sep 18 21:46:17 2007 From: donna <@t> milestonemed.com (Donna Willis) Date: Wed Sep 19 09:46:15 2007 Subject: FW: [Histonet] Microwaves Message-ID: Charles, Thought I would e-mail you to let you know that I contacted the below mentioned folks at CAP and received a different answer than you to the CAP question ANP.27170 "Are microwave devices used in accordance with manufacturer's instructions?" I was told that you should only use any equipment in the lab according to manufacture instructions. Since I have given workshops on using microwave in the lab for the past 9 years, I try to keep up with the manuals from microwave manufacture. I currently have manuals from the following household microwave manufactures: Sears Kenmore, Amana, Frigidaire, GE, Maytag, West Bend and Sanyo. All the manuals include a statement in the Safety Instructions that reads: "Use this appliance ONLY for its intended use as described in this manual. Do not use corrosive chemicals or vapors in this appliance. This type of oven is specifically designed to heat, cook or dry food. It is not designed for industrial or laboratory use." I have a manual from Sanyo from the late 80's that state in the Safety Instructions "Use this appliance only for its intended use as described in this manual." Inside the manual only mentions using for cooking, defrosting and reheating food. I suggest that you contact CAP again and get something in writing from them that it is OK to use your household microwave for laboratory procedures. If you do find out different information, would you please post it to the Histonet? I'm afraid that labs are going to receive deficiencies thinking that it is OK to use Household units when it isn't Sincerely, Donna Willis,HT(ASCP)HTL Milestone Medical North American Application Manager 2100 N. Hwy 360 Suite 506 Grand Prairie, Tx 75050 972-606-9986 office 214-725-6184 cell -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles.Embrey Sent: Wednesday, September 05, 2007 11:38 AM To: Kathleen Boozer; JR R; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Microwaves When CAP called me after my inspection the inspector said that "manufacturer's Instructions" don't say food only. He said that it applied to the safety warnings about use. If I hadn't gotten the word directly from CAP I would still be confused but I am still using my kitchen microwave to heat simple reagents and do special stains with their blessing. My first contact from CAP was Ed Gruber and he had me fax my information to Wilson Kung. Mr. Kung can be reached at 800-323-4040 X-7493 Charles Embrey, PA(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathleen Boozer Sent: Tuesday, September 04, 2007 8:38 PM To: JR R; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Microwaves CAP now says "in accordance with manufacturer's instructions". In other words, food only, no chemicals. How do I get away with that? >>> JR R 09/04/2007 16:58 >>> I use a plain old GE turntable microwave for that. It was like $150.00. You do want the turntable kind and with a digital timer is best. What you want to do is test the microwave to see exactly how long it takes to heat your solution to the required temperature in the exact container (staining tub, probably?) that you will be using. Jerry L. Ricks Research Scientist U.W. Medicine at South Lake Union 815 Mercer Street Seattle, WA 98109 (206)-685-7190> Date: Tue, 4 Sep 2007 16:23:38 -0700> From: BoozerKA@ah.org> To: histonet@lists.utsouthwestern.edu> Subject: [Histonet] Microwaves> > What is the best microwave for a small lab using it only for heating Bouin's and Silver Nitrate for special stains? I just can't believe I would have to spend $30,000+ or slow down and use a waterbath.> > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Connect to the next generation of MSN Messenger http://imagine-msn.com/messenger/launch80/default.aspx?locale=en-us&sour ce=wlmailtagline_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MVaughan4 <@t> ucok.edu Wed Sep 19 10:08:23 2007 From: MVaughan4 <@t> ucok.edu (MVaughan4@ucok.edu) Date: Wed Sep 19 10:08:58 2007 Subject: [Histonet] How to make Histonet work for you aka the Rodney King corollary In-Reply-To: Message-ID: Have your messages sent in Digest form. On the first screen you will receive something that looks like the following list. You can quickly scroll through the list to see that nothing interests you, then you can simply delete the message. Not everyone is interested in everything on the list, whether government-sanctioned or not. I think a good compromise would be to have the off-topic fun stuff on Fridays as we have seen done in the past. Mel 1. Re:HIF1a (wael swelam) 2. RE: {SPAM?} Re: [Histonet] I think I will give it an acronym (Kemlo Rogerson) 3. OT threads (louise renton) 4. RE: OT threads (Laurie Reilly) 5. RE: OT threads (Joseph Kapler) 6. workstation ergonomics (karenadams@comcast.net) 7. RE: {SPAM?} Re: [Histonet] Football and Flaming (Baldridge, Lee Ann) 8. Need Your Help to Find a Cytology Supervisor (Robert Garhart) 9. RE: Need Your Help to Find a Cytology Supervisor (Inman, Anna) 10. What are those things called? (Douglas D Deltour) 11. RE: Re: Lymph node fixative/enhance solutions (Marshall Terry Dr, Consultant Histopathologist) 12. Re: OT threads (koellingr@comcast.net) 13. RE: {SPAM?} [Histonet] What are those things called? (Douglas D Deltour) 14. Commentary (Joe Nocito) 15. Re: OT threads (Joe Nocito) 16. Re: OT threads (Larry Woody) 17. Re: Commentary (Larry Woody) 18. RE: OTT threads (Edwards, R.E.) 19. RE: OT threads (Joe's not dull) (Hofecker, Jennifer L) 20. Re: Floor in a pathology laboratory (Joe Nocito) 21. Off Topic (Judy Collins) Melville B. Vaughan, Ph. D. Assistant Professor of Biology Campus Coordinator, Sure-Step program University of Central Oklahoma 100 N. University Drive Edmond, OK 73034 http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm ----------------------------------------- **CONFIDENTIALITY** -This email (including any attachments) may contain confidential, proprietary and privileged information. Any unauthorized disclosure or use of this information is prohibited. From histocontract <@t> gmail.com Wed Sep 19 10:13:04 2007 From: histocontract <@t> gmail.com (Human Resources) Date: Wed Sep 19 10:13:14 2007 Subject: [Histonet] Histology Contractor - 4=6 Message-ID: Temporary Histology Contractor needed ? 4-6 months We are a well-known biotechnology company located in Silicon Valley, CA. We are looking for an experienced histotechnician to help out in the histology lab in a dynamic research setting. The successful candidate should have experience with basic histology procedures such as tissue trimming, processing, paraffin embedding, cutting, and staining with H&E, as well as experience in common special stains, and a facility with cryosectioning. Qualified candidates must have a minimum of 3-5 years relevant experience. Certification in HT and in phlebotomy would be a plus. To apply, please send resume to *histocontract@gmail.com.* From ander093 <@t> tc.umn.edu Wed Sep 19 10:44:45 2007 From: ander093 <@t> tc.umn.edu (LuAnn Anderson) Date: Wed Sep 19 10:50:01 2007 Subject: [Histonet] RE: Histonet Digest, Vol 46, Issue 36 In-Reply-To: <3E76917CB874EF4DB6DF79DD5CEBC1171B343934@nahqmails9.cytyc. com> References: <3E76917CB874EF4DB6DF79DD5CEBC1171B343934@nahqmails9.cytyc.com> Message-ID: Emails At 08:54 AM 9/19/2007, Cote, Laurie wrote: >Folks, >I rarely post on Histonet but I read it >avidly. The off topic posts CAN be annoying. >I've been considering something for awhile and I >wonder how you all would feel about this. >I belong to a horse board called UDBB. I log in >everyday and can read topics that I care about >and none that I don't. I'm thinking Histonet ought to go that way as well. >I picture several different areas to check into. >Something like H & E, routine sectioning, >research oriented topics, IHC and off topics. >Something like that. >I'd be willing to host such a thing on my >website www.mohsop.com. But, I wonder if you >guys would log into something like that or do you prefer the emails. > >Laurie > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] >On Behalf Of histonet-request@lists.utsouthwestern.edu >Sent: Wednesday, September 19, 2007 9:47 AM >To: histonet@lists.utsouthwestern.edu >Subject: Histonet Digest, Vol 46, Issue 36 > >Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > >To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > >You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > >When replying, please edit your Subject line so it is more specific >than "Re: Contents of Histonet digest..." > > >Today's Topics: > > 1. Re:HIF1a (wael swelam) > 2. RE: {SPAM?} Re: [Histonet] I think I will give it an acronym > (Kemlo Rogerson) > 3. OT threads (louise renton) > 4. RE: OT threads (Laurie Reilly) > 5. RE: OT threads (Joseph Kapler) > 6. workstation ergonomics (karenadams@comcast.net) > 7. RE: {SPAM?} Re: [Histonet] Football and Flaming > (Baldridge, Lee Ann) > 8. Need Your Help to Find a Cytology Supervisor (Robert Garhart) > 9. RE: Need Your Help to Find a Cytology Supervisor (Inman, Anna) > 10. What are those things called? (Douglas D Deltour) > 11. RE: Re: Lymph node fixative/enhance solutions > (Marshall Terry Dr, Consultant Histopathologist) > 12. Re: OT threads (koellingr@comcast.net) > 13. RE: {SPAM?} [Histonet] What are those things called? > (Douglas D Deltour) > 14. Commentary (Joe Nocito) > 15. Re: OT threads (Joe Nocito) > 16. Re: OT threads (Larry Woody) > 17. Re: Commentary (Larry Woody) > 18. RE: OTT threads (Edwards, R.E.) > 19. RE: OT threads (Joe's not dull) (Hofecker, Jennifer L) > 20. Re: Floor in a pathology laboratory (Joe Nocito) > 21. Off Topic (Judy Collins) > > >---------------------------------------------------------------------- > >Message: 1 >Date: Wed, 19 Sep 2007 09:48:35 +0300 >From: "wael swelam" >Subject: Re:[Histonet] HIF1a >To: Histonet@lists.utsouthwestern.edu >Message-ID: > <96566f7e0709182348u3b6db034l71b09e70b1256a3e@mail.gmail.com> >Content-Type: text/plain; charset=ISO-8859-1 > >Peace be upon you, >I tried mouse monoclonal antibody against HIF-1 a from Chemicon >International Inc. (Temecula, CA, USA, 1:100) and It was working nicely, I >would recommend you to use Tris-EDTA for retrival. >Good luck > > >-- >Wael Mohamed Swelam >BDS (Egypt), Oral Pathology MSc (Egypt), Oral Pathology PhD (Japan), >Ass. Prof. Oral Biomedical Science dept. Division of Oral Pathology, >Faculty of Dentistry, King Faisal University, Dammam, >Saudi Arabia, >P.O: 1982/3441, >http://myprofile.cos.com/wmswelam > > >------------------------------ > >Message: 2 >Date: Wed, 19 Sep 2007 07:53:48 +0100 >From: "Kemlo Rogerson" >Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym >To: "Dolores Townsend" , "Emily Sours" > , > , "Douglas > D Deltour" >Message-ID: > ><86ADE4EB583CE64799A9924684A0FBBF0222ED29@wahtntex2.waht.swest.nhs.uk> >Content-Type: text/plain; charset="us-ascii" > >Football in America, you don't play it do you. Don't you play something >called Soccer? God only knows what that is? What's that game you play >where you get little diddy men and encase them in plastic with big >shoulders and they sorta run at each other, then fall down? Bit like >Rugby but for Wussies? > >Kemlo Rogerson >Pathology Manager >DD 01934 647057 or extension 3311 >Mob 07749 754194; Pager 07659 597107; > > >I want to work for a company that contributes to and is part of the >community. I want something not just to invest in. I want something to >believe in. --Anita Roddick > >This e-mail is confidential and privileged. If you are not the intended >recipient please accept my apologies; please do not disclose, copy or >distribute information in this e-mail or take any action in reliance on >its contents: to do so is strictly prohibited and may be unlawful. >Please inform me that this message has gone astray before deleting it. >Thank you for your co-operation > > > > > >------------------------------ > >Message: 3 >Date: Wed, 19 Sep 2007 09:22:59 +0200 >From: "louise renton" >Subject: [Histonet] OT threads >To: Histonet@lists.utsouthwestern.edu >Message-ID: > >Content-Type: text/plain; charset=ISO-8859-1 > >You know a little tolerance goes a long way. > >In the many years since the inception of Histonet, I can count on the >fingers of one hand how many times the threads have gone off course. >Whether or not they stay on course is a matter of opinion. > >Should European, Australians & South Africans all jump up & down when CLIA, >CAP or HT accreditation & state licensure is mentioned and discussed at >length? This has no bearing on histology in these countries. So come on >guys, lighten up & put things into perspective. Remember the adage "All work >& no play makes Jack a dull boy" > > > >Louise Renton (in asbestos suit)* >* do not try this in your lab without prior consultation with safety >officers, CAP, JHCO, etc > > >Bone Research Unit >University of the Witwatersrand >Johannesburg >South Africa >"There are nights when the wolves are silent and only the moon howls". >George Carlin >No trees were killed in the sending of this message. >However, many electrons were terribly inconvenienced. > > >------------------------------ > >Message: 4 >Date: Wed, 19 Sep 2007 18:06:29 +1000 >From: Laurie Reilly >Subject: RE: [Histonet] OT threads >To: "'louise renton'" , > >Message-ID: <002f01c7fa93$fbb04c00$5255db89@health.ad.jcu.edu.au> >Content-Type: text/plain; charset="us-ascii" > >Good for you ,Louise. OT, in moderation, is fine by me. > >Regards, Laurie. > >Mr. Laurie REILLY >Histopathology >School of Veterinary and Biomedical Sciences >James Cook University >Townsville Qld. 4811 >Australia. > >Phone 07 4781 4468 > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise >renton >Sent: Wednesday, 19 September 2007 5:23 PM >To: Histonet@lists.utsouthwestern.edu >Subject: [Histonet] OT threads > >You know a little tolerance goes a long way. > >In the many years since the inception of Histonet, I can count on the >fingers of one hand how many times the threads have gone off course. >Whether or not they stay on course is a matter of opinion. > >Should European, Australians & South Africans all jump up & down when CLIA, >CAP or HT accreditation & state licensure is mentioned and discussed at >length? This has no bearing on histology in these countries. So come on >guys, lighten up & put things into perspective. Remember the adage "All work >& no play makes Jack a dull boy" > > > >Louise Renton (in asbestos suit)* >* do not try this in your lab without prior consultation with safety >officers, CAP, JHCO, etc > > >Bone Research Unit >University of the Witwatersrand >Johannesburg >South Africa >"There are nights when the wolves are silent and only the moon howls". >George Carlin >No trees were killed in the sending of this message. >However, many electrons were terribly inconvenienced. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > >------------------------------ > >Message: 5 >Date: Wed, 19 Sep 2007 03:32:08 -0600 >From: "Joseph Kapler" >Subject: RE: [Histonet] OT threads >To: >Message-ID: <000301c7fa9f$f3522720$d9f67560$@net> >Content-Type: text/plain; charset="us-ascii" > >I'm a Student (Mature Student) taking my courses as I work two jobs (one in >Histology), so I find Histonet as one of the best resources for information >related to my area of study. > >But I am also a major NFL fan. I enjoy a discussion from time to time on my >favorite Sport, or even some of my favorite music. > >As stated below, "all work makes joe a dull boy" is not only true, it also >leads to burnout and neurosis. Therefore, in MODERATION, a little off topic >discussion can go a long way to easing our daily stress. But Moderation is >the key. Keep it to a single thread. And remind people its off topic. > >Maybe that would help... maybe it won't ... but that's my two cents today > >Joe "DarkWolfe" Kapler > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie >Reilly >Sent: Wednesday, September 19, 2007 2:06 AM >To: 'louise renton'; Histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] OT threads > >Good for you ,Louise. OT, in moderation, is fine by me. > >Regards, Laurie. > >Mr. Laurie REILLY >Histopathology >School of Veterinary and Biomedical Sciences >James Cook University >Townsville Qld. 4811 >Australia. > >Phone 07 4781 4468 > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise >renton >Sent: Wednesday, 19 September 2007 5:23 PM >To: Histonet@lists.utsouthwestern.edu >Subject: [Histonet] OT threads > >You know a little tolerance goes a long way. > >In the many years since the inception of Histonet, I can count on the >fingers of one hand how many times the threads have gone off course. >Whether or not they stay on course is a matter of opinion. > >Should European, Australians & South Africans all jump up & down when CLIA, >CAP or HT accreditation & state licensure is mentioned and discussed at >length? This has no bearing on histology in these countries. So come on >guys, lighten up & put things into perspective. Remember the adage "All work >& no play makes Jack a dull boy" > > > >Louise Renton (in asbestos suit)* >* do not try this in your lab without prior consultation with safety >officers, CAP, JHCO, etc > > >Bone Research Unit >University of the Witwatersrand >Johannesburg >South Africa >"There are nights when the wolves are silent and only the moon howls". >George Carlin >No trees were killed in the sending of this message. >However, many electrons were terribly inconvenienced. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > >------------------------------ > >Message: 6 >Date: Wed, 19 Sep 2007 10:35:21 +0000 >From: karenadams@comcast.net >Subject: [Histonet] workstation ergonomics >To: histonet@lists.utsouthwestern.edu >Message-ID: > ><091920071035.18970.46F0FB690002DE5400004A1A22070206539C030E0B0E020A9D0E05@comcast.net> > >Content-Type: text/plain > >We are remodeling our existing lab and I would >like input into the pros and cons of workstation >design, specifically the semi circular cutout vs >and L-shaped design in addition to dimensions >necessary to accommodate workstation >supplies.....any and all input into things that >you hate and things you love would be MUCH appreciated!!!! > >-- >Karen Adams >Pathology Laboratories West >9303 Park West Blvd >Knoxville, TN 37923 >(865) 690-2111 FAX (865) 691-1623 > >------------------------------ > >Message: 7 >Date: Wed, 19 Sep 2007 07:29:10 -0400 >From: "Baldridge, Lee Ann" >Subject: RE: {SPAM?} Re: [Histonet] Football and Flaming >To: "Patricia Adams" , "HistoNet" > >Message-ID: > <1E3324ECFB5D5F4DAFEDD17203EEBF2101358F58@iu-mssg-mbx105.ads.iu.edu> >Content-Type: text/plain; charset="us-ascii" > >Well said Patricia. I totally agree. >Oh, Go Colts!!! >Lee Ann Baldridge >IUSM >Indpls.,IN. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patricia >Adams >Sent: Tuesday, September 18, 2007 4:58 PM >To: HistoNet >Subject: RE: {SPAM?} Re: [Histonet] Football and Flaming > >I know that sometimes wading through unwanted >histonet "stuff" can be a trial. I feel that way >when someone wants to know about some procedure I >have never heard of or will never use. I do hit >the delete key. Or sometimes take the time to >learn something new. >I also really enjoy the off topic histo threads, >makes me know that all these very smart Techs are >also human and would be fun to hang around with. >So please keep the humor and wit going! >Patrica >--- Larry Woody wrote: > > > Did someone from Detroit mention a party? > > Histology people never party, we are always > > business and the fact that both words are even > > mentioned in the same sentence makes me want to > > get back to work! I can't help it, my barcode > > is not scanning properly. > > > > Douglas D Deltour wrote: > > Sorry Nancy. > > > > I understand that football is non-existent in > > Detroit. :) > > > > We will get back to the Histology Stuff. > > > > Douglas D. Deltour HT(ASCP) > > Histology Manager > > Professional Pathology Services, PC > > One Science Court > > Suite 200 > > Columbia, SC 29203 > > Office (803)252-1913 > > Fax (803)254-3262 > > Doug@ppspath.com > > >***************************************************** > > PROFESSIONAL PATHOLOGY SERVICES, PC > > NOTICE OF CONFIDENTIALITY > > This message is intended only for the use of > > the individual or entity to > > which it is addressed and may contain > > information that is privileged, > > confidential and exempt from disclosure under > > applicable law. If the reader > > of this message is not the intended recipient, > > you are hereby notified that > > any dissemination, distribution, or copying of > > this communication is > > strictly prohibited by law. If you have > > received this communication in > > error, please notify me immediately. > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > >[mailto:histonet-bounces@lists.utsouthwestern.edu] > > On Behalf Of Nancy Lemke > > Sent: Tuesday, September 18, 2007 1:57 PM > > To: Thomas Pier; > > histonet@lists.utsouthwestern.edu; > > sbreeden@nmda.nmsu.edu > > Subject: {SPAM?} Re: [Histonet] Football and > > Flaming > > > > Sorry to be a party pooper but is it possible > > to eliminate the non histology > > threads from the general list and move that > > type of communication to direct > > emails between the interested parties? It feels > > like a low-level hijacking > > when Histonet becomes engulfed in one of these > > threads. > > Thanks > > > > Nancy Lemke > > Research Coordinator > > Hermelin Brain Tumor Center > > Henry Ford Hospital > > Detroit > > -----Original message----- > > From: "Thomas Pier" tp2@medicine.wisc.edu > > Date: Tue, 18 Sep 2007 14:44:03 -0400 > > To: histonet@lists.utsouthwestern.edu > > Subject: Re: [Histonet] Football and Flaming > > > > > Sall, > > > Ummm, where do I begin. "America's Team" > > still can't match the 12 time > > > world champ Packers. That's right. 12. 12 is > > more than anyone else has. > > > Roger Staubach is no Bart Starr and Troy Aikman > > is no Brett Favre. I'm > > > not even going to start on that crackhead > > Michael Irvin. Your boy Tony > > > Romo is from Wisconsin too. It would also seem > > to me that "your" Braves > > > won the 1957 World Series over the New York > > Yankees before Atlanta stole > > > them from Milwaukee. No flame, just facts, > > there you go. > > > > > > Tom Pier > > > Proud Wisconsinite > > > > > > >>> "Breeden, Sara" 09/18/07 1:03 PM >>> > > > I've been quiet lately, mainly because as my > > vacation approaches (3 > > > days!), all **** is breaking loose (that > > happens when one is the Only > > > histotech). However, this Football Thing has > > gotten my attention. No > > > one has said anything about America's Team - > > the Dallas Cowboys! Now, > > > ya'll just have a big ol' time flaming me > > because my skin is so thick > > > from years of totally unwarranted abuse by > > lesser teams' fans that you > > > can't hurt me. Besides, I've given up that > > highly overrated physical > > > sport for the real sport of BASEBALL - where > > stealth and cunning mean so > > > much more. Go Braves!! I was further sure of > > my Sports Choice last > > > year when my friend Linda Blazek and I paid > > way too much money for two > > > tickets to see the D-Backs in PHX. But we had > > great seats! So, give me > > > your best shot and I'll try to remember I > > asked for it... Vacation > > > cures almost everything... > > > > > > > > > > > > Sally Breeden, HT(ASCP) > > > > > > NM Dept. of Agriculture > > > > > > Veterinary Diagnostic Services > > > > > > PO Box 4700 > > > > > > Albuquerque, NM 87106 > > > > > > 505-841-2576 > > > > > > > > > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > > > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > > > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > >======================================================================== >==== > > == > > CONFIDENTIALITY NOTICE: This email contains > > information from the sender that > > may be CONFIDENTIAL, LEGALLY PRIVILEGED, > > PROPRIETARY or otherwise protected > > from disclosure. This email is intended for use > > only by the person or entity > > to whom it is addressed. If you are not the > > intended recipient, any use, > > disclosure, copying, distribution, printing, or > > any action taken in reliance > > on the contents of this email, is strictly > > prohibited. If you received this > > email in error, please contact the sending > > party by reply email, delete the > > email from your computer system and shred any > > paper copies. > > > > Note to Patients: There are a number of risks > > you should consider before > > using e-mail to communicate with us. See our > > Privacy Policy and Henry Ford > > My Health at www.henryford.com for more > > detailed information. If you do not > > believe that our policy gives you the privacy > > and security protection you > > need, do not send e-mail or Internet > > communications to us. > > > > >======================================================================== >==== > > == > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > --------------------------------- > > Tonight's top picks. What will you watch > > tonight? Preview the hottest shows on Yahoo! > > TV. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >________________________________________________________________________ >____________ >Luggage? GPS? Comic books? >Check out fitting gifts for grads at Yahoo! Search >http://search.yahoo.com/search?fr=oni_on_mail&p=graduation+gifts&cs=bz > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >------------------------------ > >Message: 8 >Date: Wed, 19 Sep 2007 04:59:22 -0700 >From: "Robert Garhart" >Subject: [Histonet] Need Your Help to Find a Cytology Supervisor >To: >Message-ID: > >Content-Type: text/plain; charset="us-ascii" > > >I know this is Histonet but I wanted to see if you could help me. I am >looking for the following: > >Position: Cytology Supervisor with FISH experience. Would be involved >in the selection and addition of new installations as a well backed lab >continues to grow. Great growth opportunities as this lab continues to >expand. > >Location: NJ > >Please pass this along to any cytology supervisors you know or point me >in the direction of a similar listserve if you know of one for the >Cytology side of things. > >Thanks for your help. > >Robert Garhart >Executive Recruiter >System 1 Search >678-342-9029 Office >rgarhart@system1.net >Website: www.system1.net > > > > >------------------------------ > >Message: 9 >Date: Wed, 19 Sep 2007 06:13:24 -0600 >From: "Inman, Anna" >Subject: RE: [Histonet] Need Your Help to Find a Cytology Supervisor >To: "Robert Garhart" , > >Message-ID: > <2925AE271EAAD440AF48FCCEB8002D090542F79C@smgmail01.smgj.sclhs.net> >Content-Type: text/plain; charset="us-ascii" > >We are also looking for a Cytology Supervisor (no FISH experience >required) > >In Grand Junction, Colorado. > >Great question - Is there a listserv for Cytology? > > > >Thank you! > > > >Anna Inman > >SMH Pathology > >(970)244-7098 > >Anna.Inman@stmarygj.org > > > > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert >Garhart >Sent: Wednesday, September 19, 2007 5:59 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Need Your Help to Find a Cytology Supervisor > > > > > >I know this is Histonet but I wanted to see if you could help me. I am > >looking for the following: > > > >Position: Cytology Supervisor with FISH experience. Would be involved > >in the selection and addition of new installations as a well backed lab > >continues to grow. Great growth opportunities as this lab continues to > >expand. > > > >Location: NJ > > > >Please pass this along to any cytology supervisors you know or point me > >in the direction of a similar listserve if you know of one for the > >Cytology side of things. > > > >Thanks for your help. > > > >Robert Garhart > >Executive Recruiter > >System 1 Search > >678-342-9029 Office > >rgarhart@system1.net > >Website: www.system1.net > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >CONFIDENTIALITY NOTICE: This e-mail message, >including any attachments, is for the sole use >of the intended recipient(s) and may contain >confidential and privileged information. Any >unauthorized review, use, disclosure or >distribution is prohibited. If you are not the >intended recipient, please contact the sender by >reply e-mail and destroy all copies of the original message. > > > > >------------------------------ > >Message: 10 >Date: Wed, 19 Sep 2007 08:28:48 -0500 >From: "Douglas D Deltour" >Subject: [Histonet] What are those things called? >To: >Message-ID: > >Content-Type: text/plain; charset="us-ascii" > >I am looking for the "cold bath" or whatever they are called for frozen >sectioning. They are the things that you put the 2-methyl butane in to >freeze the molds. Any idea on who carries them? Thanks. > > > > > >Douglas D. Deltour HT(ASCP) > >Histology Manager > >Professional Pathology Services, PC > >One Science Court > >Suite 200 > >Columbia, SC 29203 > >Office (803)252-1913 > >Fax (803)254-3262 > >Doug@ppspath.com > >***************************************************** > >PROFESSIONAL PATHOLOGY SERVICES, PC >NOTICE OF CONFIDENTIALITY >This message is intended only for the use of the individual or entity to >which it is addressed and may contain information that is privileged, >confidential and exempt from disclosure under applicable law. If the reader >of this message is not the intended recipient, you are hereby notified that >any dissemination, distribution, or copying of this communication is >strictly prohibited by law. If you have received this communication in >error, please notify me immediately. > > > > > >------------------------------ > >Message: 11 >Date: Wed, 19 Sep 2007 13:50:19 +0100 >From: "Marshall Terry Dr, Consultant Histopathologist" > >Subject: RE: [Histonet] Re: Lymph node fixative/enhance solutions >To: "Robert Richmond" , > >Message-ID: > <407F05A128805F4C879A33DBA32E618E01895061@TRFT-EX01.xRothGen.nhs.uk> >Content-Type: text/plain; charset="us-ascii" > >Our associated teaching hospital uses xylene to clear the fat for nodes. >Has anybody experience of this? > >Terry > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert >Richmond >Sent: 11 September 2007 18:53 >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Re: Lymph node fixative/enhance solutions > >Cindi Robinson, HT (ASCP) in South Dakota asks: > > >>Our pathologists would like us to start using a solution to help >with the dissection and identification of lymph nodes at gross. - We do >use Pen-Fix but it doesn't dissolve the fat like the paths would like.<< > >And Dana Dittus at Polysciences notes: > >>Polysciences,Inc manufactures Hartmanns Fixative which turns lymph >nodes white. For more info see www.polysciences.com << > >It's easy and cheap to prepare Davidson's fixative (same as Hartmann's >fixative) - mix 3 parts water, 3 parts reagent alcohol, 2 parts strong >(37%) formalin, not the buffered kind, and 1 part glacial acetic acid. >Color it with eosin if you want to. > >When Dr. William Hartmann (later the chair of the American Board of >Pathology) was at Johns Hopkins in the early 1960's, Dr. Victor McCusick >(the medical geneticist, later chair of medicine at Hopkins) asked him >to introduce the fixative for Barr bodies (sex chromatin >bodies) that Moore and Barr had specified in their original articles on >Barr bodies around 1954. Moore and Barr specified "modified Davidson's >fixative", which Bill Hartmann introduced. It came into rather >widespread use at Hopkins, and Hartmann's name stuck to it. I think the >name should not be used, because of possible disastrous confusion with >Hartmann's solution, lactated Ringer's solution, introduced into >pediatric practice in the 1930's by Dr. Alexis Hartmann Sr. at >Washington University in St.Louis. Dr. Alexis Hartmann was one of my >clinical teachers, right before he retired around 1964 (sorry about all >this name dropping). > >John Kiernan and I discussed Davidson's fixative in this forum several >years ago. He noted that it is not really a rational formula. He hoped >to have the time to research the fixative in Davidson's papers, since it >seems never to have been published. > >Getting back to the actual subject, I've used Dissect Aid (Decal >Corporation) with good results - got a bottle of it on the shelf right >now, in fact. In my brief experience with Penn-Fix, it did not disclose >lymph nodes well. > >Let me support your pathologists very strongly in this endeavor. Just >one positive lymph node upstages a colon cancer and makes chemotherapy >mandatory. And in colon cancer, those postive lymph nodes can be the >size of pinheads. > >Bob Richmond >Samurai Pathologist >Knoxville TN > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >------------------------------ > >Message: 12 >Date: Wed, 19 Sep 2007 12:54:31 +0000 >From: koellingr@comcast.net >Subject: Re: [Histonet] OT threads >To: "louise renton" , > Histonet@lists.utsouthwestern.edu >Message-ID: > ><091920071254.29636.46F11C0700051F30000073C422007621949D09020704040A0105@comcast.net> > >Content-Type: text/plain > >OT threads do not bother me one way or the >other. I just shake my head, ignore and move on >to more important things. But I am curious as a >biologist, not even counting the talk of CLIA, >CAP or HT topics and just counting OT since the >inception of the HistoNet regarding guns, >Dieties, parties, personal insults, perversions, >political incorrectness, recipes, ponds, lakes, >scams, vacations, tirades, sexual innuendo, >firings, football/soccer, scoldings and multiple >topics too difficult to categorize, someone has >several hundred fingers, possibly 500, on just one hand? > >dull Ray Koelling >PhenoPath Labs >Seattle, WA > > >-------------- Original message -------------- >From: "louise renton" > > > You know a little tolerance goes a long way. > > > > In the many years since the inception of Histonet, I can count on the > > fingers of one hand how many times the threads have gone off course. > > Whether or not they stay on course is a matter of opinion. > > > > Should European, Australians & South Africans > all jump up & down when CLIA, > > CAP or HT accreditation & state licensure is mentioned and discussed at > > length? This has no bearing on histology in these countries. So come on > > guys, lighten up & put things into > perspective. Remember the adage "All work > > & no play makes Jack a dull boy" > > > > > > > > Louise Renton (in asbestos suit)* > > * do not try this in your lab without prior consultation with safety > > officers, CAP, JHCO, etc > > > > > > Bone Research Unit > > University of the Witwatersrand > > Johannesburg > > South Africa > > "There are nights when the wolves are silent and only the moon howls". > > George Carlin > > No trees were killed in the sending of this message. > > However, many electrons were terribly inconvenienced. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >------------------------------ > >Message: 13 >Date: Wed, 19 Sep 2007 08:54:47 -0500 >From: "Douglas D Deltour" >Subject: RE: {SPAM?} [Histonet] What are those things called? >To: >Message-ID: > >Content-Type: text/plain; charset="us-ascii" > >Never mind I found it. I hate it when I do that. > >Douglas D. Deltour HT(ASCP) >Histology Manager >Professional Pathology Services, PC >One Science Court >Suite 200 >Columbia, SC 29203 >Office (803)252-1913 >Fax (803)254-3262 >Doug@ppspath.com >***************************************************** >PROFESSIONAL PATHOLOGY SERVICES, PC >NOTICE OF CONFIDENTIALITY >This message is intended only for the use of the individual or entity to >which it is addressed and may contain information that is privileged, >confidential and exempt from disclosure under applicable law. If the reader >of this message is not the intended recipient, you are hereby notified that >any dissemination, distribution, or copying of this communication is >strictly prohibited by law. If you have received this communication in >error, please notify me immediately. > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D >Deltour >Sent: Wednesday, September 19, 2007 8:29 AM >To: histonet@lists.utsouthwestern.edu >Subject: {SPAM?} [Histonet] What are those things called? > >I am looking for the "cold bath" or whatever they are called for frozen >sectioning. They are the things that you put the 2-methyl butane in to >freeze the molds. Any idea on who carries them? Thanks. > > > > > >Douglas D. Deltour HT(ASCP) > >Histology Manager > >Professional Pathology Services, PC > >One Science Court > >Suite 200 > >Columbia, SC 29203 > >Office (803)252-1913 > >Fax (803)254-3262 > >Doug@ppspath.com > >***************************************************** > >PROFESSIONAL PATHOLOGY SERVICES, PC >NOTICE OF CONFIDENTIALITY >This message is intended only for the use of the individual or entity to >which it is addressed and may contain information that is privileged, >confidential and exempt from disclosure under applicable law. If the reader >of this message is not the intended recipient, you are hereby notified that >any dissemination, distribution, or copying of this communication is >strictly prohibited by law. If you have received this communication in >error, please notify me immediately. > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > >------------------------------ > >Message: 14 >Date: Wed, 19 Sep 2007 08:16:23 -0500 >From: "Joe Nocito" >Subject: [Histonet] Commentary >To: "histonet" >Message-ID: <009f01c7fabf$492ecd50$0202a8c0@yourxhtr8hvc4p> >Content-Type: text/plain; charset="iso-8859-1" > >I see by some of the comments that recent topics >have upset some people. It is a sad day when we >take ourselves so seriously that we can not >diverse for a day or two. We work hard day in >and day out and with the high quality and time >constraints, we are constantly under pressure. > Is it so bad to stop and smell the roses > once in a while? Ninety-nine per cent of the > Histonet does deal with histology. Is it so bad > to once in a while to talk about something else > such as recipes, football, dresses or perfumes? > As a manager, I encourage my techs and > students to develop interests outside of > histology. See, I didn't take my own advice and > became so burnt out that I had to quit my job > and take the summer off. This was evident when > I had 7 1/2 weeks of vacation on the books. > I have learned a lot from this forum and > hopefully I have given some instruction back. > This includes histology and non-histology > topics. Let's lighten up a bit, it's healthy. > >Joe Nocito BS, PA, HT(ASCP)QIHC >San Antonio, TX > >------------------------------ > >Message: 15 >Date: Wed, 19 Sep 2007 08:18:44 -0500 >From: "Joe Nocito" >Subject: Re: [Histonet] OT threads >To: , >Message-ID: <00d701c7fabf$9b7bd530$0202a8c0@yourxhtr8hvc4p> >Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > >hey, >I'm not dull > >JTT >----- Original Message ----- >From: "Joseph Kapler" >To: >Sent: Wednesday, September 19, 2007 4:32 AM >Subject: RE: [Histonet] OT threads > > > > I'm a Student (Mature Student) taking my courses as I work two jobs (one > > in > > Histology), so I find Histonet as one of the best resources for > > information > > related to my area of study. > > > > But I am also a major NFL fan. I enjoy a discussion from time to time on > > my > > favorite Sport, or even some of my favorite music. > > > > As stated below, "all work makes joe a dull boy" is not only true, it also > > leads to burnout and neurosis. Therefore, in MODERATION, a little off > > topic > > discussion can go a long way to easing our daily stress. But Moderation is > > the key. Keep it to a single thread. And remind people its off topic. > > > > Maybe that would help... maybe it won't ... but that's my two cents today > > > > Joe "DarkWolfe" Kapler > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie > > Reilly > > Sent: Wednesday, September 19, 2007 2:06 AM > > To: 'louise renton'; Histonet@lists.utsouthwestern.edu > > Subject: RE: [Histonet] OT threads > > > > Good for you ,Louise. OT, in moderation, is fine by me. > > > > Regards, Laurie. > > > > Mr. Laurie REILLY > > Histopathology > > School of Veterinary and Biomedical Sciences > > James Cook University > > Townsville Qld. 4811 > > Australia. > > > > Phone 07 4781 4468 > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise > > renton > > Sent: Wednesday, 19 September 2007 5:23 PM > > To: Histonet@lists.utsouthwestern.edu > > Subject: [Histonet] OT threads > > > > You know a little tolerance goes a long way. > > > > In the many years since the inception of Histonet, I can count on the > > fingers of one hand how many times the threads have gone off course. > > Whether or not they stay on course is a matter of opinion. > > > > Should European, Australians & South Africans all jump up & down when > > CLIA, > > CAP or HT accreditation & state licensure is mentioned and discussed at > > length? This has no bearing on histology in these countries. So come on > > guys, lighten up & put things into perspective. Remember the adage "All > > work > > & no play makes Jack a dull boy" > > > > > > > > Louise Renton (in asbestos suit)* > > * do not try this in your lab without prior consultation with safety > > officers, CAP, JHCO, etc > > > > > > Bone Research Unit > > University of the Witwatersrand > > Johannesburg > > South Africa > > "There are nights when the wolves are silent and only the moon howls". > > George Carlin > > No trees were killed in the sending of this message. > > However, many electrons were terribly inconvenienced. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > >------------------------------ > >Message: 16 >Date: Wed, 19 Sep 2007 06:21:58 -0700 (PDT) >From: Larry Woody >Subject: Re: [Histonet] OT threads >To: koellingr@comcast.net, louise renton , > Histonet@lists.utsouthwestern.edu >Message-ID: <339393.60920.qm@web53608.mail.re2.yahoo.com> >Content-Type: text/plain; charset=iso-8859-1 > >I think they are counting hanging chads. > >koellingr@comcast.net wrote: OT threads do not >bother me one way or the other. I just shake my >head, ignore and move on to more important >things. But I am curious as a biologist, not >even counting the talk of CLIA, CAP or HT topics >and just counting OT since the inception of the >HistoNet regarding guns, Dieties, parties, >personal insults, perversions, political >incorrectness, recipes, ponds, lakes, scams, >vacations, tirades, sexual innuendo, firings, >football/soccer, scoldings and multiple topics >too difficult to categorize, someone has several >hundred fingers, possibly 500, on just one hand? > >dull Ray Koelling >PhenoPath Labs >Seattle, WA > > >-------------- Original message -------------- >From: "louise renton" > > > You know a little tolerance goes a long way. > > > > In the many years since the inception of Histonet, I can count on the > > fingers of one hand how many times the threads have gone off course. > > Whether or not they stay on course is a matter of opinion. > > > > Should European, Australians & South Africans > all jump up & down when CLIA, > > CAP or HT accreditation & state licensure is mentioned and discussed at > > length? This has no bearing on histology in these countries. So come on > > guys, lighten up & put things into > perspective. Remember the adage "All work > > & no play makes Jack a dull boy" > > > > > > > > Louise Renton (in asbestos suit)* > > * do not try this in your lab without prior consultation with safety > > officers, CAP, JHCO, etc > > > > > > Bone Research Unit > > University of the Witwatersrand > > Johannesburg > > South Africa > > "There are nights when the wolves are silent and only the moon howls". > > George Carlin > > No trees were killed in the sending of this message. > > However, many electrons were terribly inconvenienced. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >--------------------------------- >Take the Internet to Go: Yahoo!Go puts the >Internet in your pocket: mail, news, photos & more. > >------------------------------ > >Message: 17 >Date: Wed, 19 Sep 2007 06:25:44 -0700 (PDT) >From: Larry Woody >Subject: Re: [Histonet] Commentary >To: Joe Nocito , histonet > >Message-ID: <127664.31138.qm@web53609.mail.re2.yahoo.com> >Content-Type: text/plain; charset=iso-8859-1 > >Agreed Joe, it's called work/life balance but >when your work is your life like so many people >in America, it's impossible to achieve balance. > >Joe Nocito wrote: I see >by some of the comments that recent topics have >upset some people. It is a sad day when we take >ourselves so seriously that we can not diverse >for a day or two. We work hard day in and day >out and with the high quality and time >constraints, we are constantly under pressure. >Is it so bad to stop and smell the roses once in >a while? Ninety-nine per cent of the Histonet >does deal with histology. Is it so bad to once >in a while to talk about something else such as >recipes, football, dresses or perfumes? >As a manager, I encourage my techs and students >to develop interests outside of histology. See, >I didn't take my own advice and became so burnt >out that I had to quit my job and take the >summer off. This was evident when I had 7 1/2 weeks of vacation on the books. >I have learned a lot from this forum and >hopefully I have given some instruction back. >This includes histology and non-histology >topics. Let's lighten up a bit, it's healthy. > >Joe Nocito BS, PA, HT(ASCP)QIHC >San Antonio, TX >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >--------------------------------- >Be a better Heartthrob. Get better relationship >answers from someone who knows. >Yahoo! Answers - Check it out. > >------------------------------ > >Message: 18 >Date: Wed, 19 Sep 2007 14:25:47 +0100 >From: "Edwards, R.E." >Subject: RE: [Histonet] OTT threads >To: "Joe Nocito" , , > >Message-ID: > >Content-Type: text/plain; charset="US-ASCII" > > >Perhaps, just a touch opaque?. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe >Nocito >Sent: 19 September 2007 14:19 >To: drkwolfe@telus.net; Histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] OT threads > >hey, >I'm not dull > >JTT >----- Original Message ----- >From: "Joseph Kapler" >To: >Sent: Wednesday, September 19, 2007 4:32 AM >Subject: RE: [Histonet] OT threads > > > > I'm a Student (Mature Student) taking my courses as I work two jobs >(one > > in > > Histology), so I find Histonet as one of the best resources for > > information > > related to my area of study. > > > > But I am also a major NFL fan. I enjoy a discussion from time to time >on > > my > > favorite Sport, or even some of my favorite music. > > > > As stated below, "all work makes joe a dull boy" is not only true, it >also > > leads to burnout and neurosis. Therefore, in MODERATION, a little off > > topic > > discussion can go a long way to easing our daily stress. But >Moderation is > > the key. Keep it to a single thread. And remind people its off topic. > > > > Maybe that would help... maybe it won't ... but that's my two cents >today > > > > Joe "DarkWolfe" Kapler > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie > > Reilly > > Sent: Wednesday, September 19, 2007 2:06 AM > > To: 'louise renton'; Histonet@lists.utsouthwestern.edu > > Subject: RE: [Histonet] OT threads > > > > Good for you ,Louise. OT, in moderation, is fine by me. > > > > Regards, Laurie. > > > > Mr. Laurie REILLY > > Histopathology > > School of Veterinary and Biomedical Sciences > > James Cook University > > Townsville Qld. 4811 > > Australia. > > > > Phone 07 4781 4468 > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise > > renton > > Sent: Wednesday, 19 September 2007 5:23 PM > > To: Histonet@lists.utsouthwestern.edu > > Subject: [Histonet] OT threads > > > > You know a little tolerance goes a long way. > > > > In the many years since the inception of Histonet, I can count on the > > fingers of one hand how many times the threads have gone off course. > > Whether or not they stay on course is a matter of opinion. > > > > Should European, Australians & South Africans all jump up & down when > > CLIA, > > CAP or HT accreditation & state licensure is mentioned and discussed >at > > length? This has no bearing on histology in these countries. So come >on > > guys, lighten up & put things into perspective. Remember the adage >"All > > work > > & no play makes Jack a dull boy" > > > > > > > > Louise Renton (in asbestos suit)* > > * do not try this in your lab without prior consultation with safety > > officers, CAP, JHCO, etc > > > > > > Bone Research Unit > > University of the Witwatersrand > > Johannesburg > > South Africa > > "There are nights when the wolves are silent and only the moon howls". > > George Carlin > > No trees were killed in the sending of this message. > > However, many electrons were terribly inconvenienced. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >------------------------------ > >Message: 19 >Date: Wed, 19 Sep 2007 08:25:53 -0500 >From: "Hofecker, Jennifer L" >Subject: RE: [Histonet] OT threads (Joe's not dull) >To: "Joe Nocito" , > >Message-ID: > <898D946569A27444B65667A49C074052F7296A@mailbe06.mc.vanderbilt.edu> >Content-Type: text/plain; charset="us-ascii" > > >I'll second that! Joe and "dull" in the same sentence, that's a first. >Oh, man - Now I've jumped into OT land, too! Joe, look what you made me >do :) > >Looking for flame retardant suit... >Jennifer >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe >Nocito >Sent: Wednesday, September 19, 2007 8:19 AM >To: drkwolfe@telus.net; Histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] OT threads > > >hey, >I'm not dull > >JTT >----- Original Message ----- >From: "Joseph Kapler" >To: >Sent: Wednesday, September 19, 2007 4:32 AM >Subject: RE: [Histonet] OT threads > > > > I'm a Student (Mature Student) taking my courses as I work two jobs > > (one > > in > > Histology), so I find Histonet as one of the best resources for > > information > > related to my area of study. > > > > But I am also a major NFL fan. I enjoy a discussion from time to time > > on > > my > > favorite Sport, or even some of my favorite music. > > > > As stated below, "all work makes joe a dull boy" is not only true, it > > also leads to burnout and neurosis. Therefore, in MODERATION, a little > > > off topic discussion can go a long way to easing our daily stress. But > > > Moderation is the key. Keep it to a single thread. And remind people > > its off topic. > > > > Maybe that would help... maybe it won't ... but that's my two cents > > today > > > > Joe "DarkWolfe" Kapler > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie > > > Reilly > > Sent: Wednesday, September 19, 2007 2:06 AM > > To: 'louise renton'; Histonet@lists.utsouthwestern.edu > > Subject: RE: [Histonet] OT threads > > > > Good for you ,Louise. OT, in moderation, is fine by me. > > > > Regards, Laurie. > > > > Mr. Laurie REILLY > > Histopathology > > School of Veterinary and Biomedical Sciences > > James Cook University > > Townsville Qld. 4811 > > Australia. > > > > Phone 07 4781 4468 > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise > > > renton > > Sent: Wednesday, 19 September 2007 5:23 PM > > To: Histonet@lists.utsouthwestern.edu > > Subject: [Histonet] OT threads > > > > You know a little tolerance goes a long way. > > > > In the many years since the inception of Histonet, I can count on the > > fingers of one hand how many times the threads have gone off course. > > Whether or not they stay on course is a matter of opinion. > > > > Should European, Australians & South Africans all jump up & down when > > CLIA, > > CAP or HT accreditation & state licensure is mentioned and discussed >at > > length? This has no bearing on histology in these countries. So come >on > > guys, lighten up & put things into perspective. Remember the adage >"All > > work > > & no play makes Jack a dull boy" > > > > > > > > Louise Renton (in asbestos suit)* > > * do not try this in your lab without prior consultation with safety > > officers, CAP, JHCO, etc > > > > > > Bone Research Unit > > University of the Witwatersrand > > Johannesburg > > South Africa > > "There are nights when the wolves are silent and only the moon howls". > > > George Carlin No trees were killed in the sending of this message. > > However, many electrons were terribly inconvenienced. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >------------------------------ > >Message: 20 >Date: Wed, 19 Sep 2007 08:27:31 -0500 >From: "Joe Nocito" >Subject: Re: [Histonet] Floor in a pathology laboratory >To: "Gayle Callis" , "Rene J Buesa" > , >Message-ID: <011f01c7fac0$d6778390$0202a8c0@yourxhtr8hvc4p> >Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=response > >I had some carpets like this and is was a bear to work with in the embedding >area. The wheels of the chairs kept causing the carpet to rise up, causing a >tripping hazard. Anyone else experience this? By the microtomes, the carpet >worked fine. > >JTT >----- Original Message ----- >From: "Gayle Callis" >To: "Rene J Buesa" ; >Sent: Tuesday, September 18, 2007 3:41 PM >Subject: Re: [Histonet] Floor in a pathology laboratory > > >I agree with Rene. We also have commerical rugs (heavy duty, very low >carpet, inexpensive, and are the kind found in entry ways of >buildings). We place these in front of microtome areas, sinks and >processor, chairs roll on them without resistance. They can be vacuumed >to pick up paraffin shavings, and when eventually trashed, merely >replaced. We had an incident on a hard floor, as paraffin seems to >permeate and coat all surfaces. A visiting post doc slipped, and if he >hadn't grabbed a door handle, would have cracked back of his skull on our >hard floor. Our janitors here would NEVER think to mop a floor, but they >do vacuum these little carpets. Perhaps clinical labs have better >regulations for cleaning histology lab areas than our research facility ( I >work in a double wide trailer house! also called a modular) has available. > >If you can't use carpets, then consider the sticky peel a away mats under >your microtoming areas, this catches trimmings that seem to fly around >willy nilly, no matter what one does. These are also used at entry ways of >areas where dust, dirt and/or prion associated work is done. > >Be sure you ask for acid resistant sinks, that are large, deep and enough >of them to do the work necessary, staining, grossing areas, etc, etc. A >lab with two sinks like ours is NOT a good situation. Also, ask that the >the microtoming area has decent bright lighting and NO air vents just about >the microtome area. > >Good luck and congratulations on being able to design a new laboratory. > >At 02:14 PM 9/18/2007, you wrote: > >Chemicals resistant, anti-slip, and ascrubbable to eliminate paraffin. > > Ren? J. > > > >" > >Gayle Callis >MT,HT,HTL(ASCP) >Research Histopathology Supervisor >Veterinary Molecular Biology >Montana State University - Bozeman >PO Box 173610 >Bozeman MT 59717-3610 > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > >------------------------------ > >Message: 21 >Date: Wed, 19 Sep 2007 09:32:35 -0400 >From: "Judy Collins" >Subject: [Histonet] Off Topic >To: >Message-ID: > >Content-Type: text/plain; charset="us-ascii" > > >From my perspective, my company pays my salary to do histology and I use >the histonet during work hours because it is histology related. My >stress relief needs to be on my own time. Therefore, I think the >non-histology chatter should be kept to minimum. A couple of comments >are one thing, but sometimes it is excessive. > >Judy Collins >Palm Beach Pathology > > > >------------------------------ > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >End of Histonet Digest, Vol 46, Issue 36 >**************************************** > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abright <@t> brightinstruments.com Wed Sep 19 10:20:35 2007 From: abright <@t> brightinstruments.com (Alan Bright) Date: Wed Sep 19 10:52:29 2007 Subject: [Histonet] Cutting rat brains References: <002001c7f66e$e4eea8f0$aecbfad0$@cs.nsw.gov.au> Message-ID: Dear Rosalba, What would save you much time is to have motor driven cryomicrotome ( Cryostat ) plus a section counter to be set to the required amount of sections and then stop the automatic trimming. . This would free up your time, enabling you to carry out other tasks while the frozen blocks are being trimmed. This is fitted to many cryostats we produce. Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com Skype: dazzle0 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rosalba Zumbo Sent: 14 September 2007 02:31 To: HistoNet Server (E-mail) Subject: [Histonet] Cutting rat brains I want to cut a frozen rat brain at around 1-3 mm intervals to form blocks. I wish to be able to return to each block at a later date to take further sections if necessary. Can anyone suggest a suitable method or piece of equipment that would aid this task? Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmconway <@t> usgs.gov Wed Sep 19 10:54:37 2007 From: cmconway <@t> usgs.gov (Carla M Conway) Date: Wed Sep 19 10:59:54 2007 Subject: [Histonet] paraffin processing problem Message-ID: Hello, I have an LX120 tissue processor. The paraffin infiltration time is programmed for three 1hour stations. Sometime last night a problem occurred during the paraffin cycle (unfortunately, we do not have a printout or record of when it happened). This morning the Fill Failure alarm was sounding and tissues were not soaking in paraffin as they should have been. There was only paraffin residue in the processing chamber, so I know that the tissues were exposed to paraffin but don't know for how long. What should I do? I do not want to overprocess them in paraffin, but don't want them underinfiltrated either. The tissues are staghorn sculpin hearts and livers. Thanks so much for your help, Carla Conway Western Fisheries Research Center 6505 NE 65th St. Seattle, WA 98115 cmconway@usgs.gov From FUNKM <@t> mercyhealth.com Wed Sep 19 11:07:13 2007 From: FUNKM <@t> mercyhealth.com (Marcia Funk) Date: Wed Sep 19 11:07:49 2007 Subject: [Histonet] Carpet in Histology Message-ID: <46F102E1.9B87.00AC.0@mercyhealth.com> I have not used or seen carpet used in a Histology lab. We are looking at remolding also. Does the paraffin vacuum up well ? We do have a concern with our floor that we have now that it does becomes slippery. Marcia From yvan_lindekens <@t> yahoo.com Wed Sep 19 11:26:29 2007 From: yvan_lindekens <@t> yahoo.com (yvan lindekens) Date: Wed Sep 19 11:26:40 2007 Subject: [Histonet] User manual for a Jung Tetrander wanted...A srvice manual would be a gift from the gods :-))) In-Reply-To: <1576150269.20070918223134@mail.ru> Message-ID: <74979.95420.qm@web30909.mail.mud.yahoo.com> If someone of you guys have one of those, don't hesitate to contact me! As usual Leica Belgium/Van Hopplynus Brussels is playing the well known authistic routine and no help is to be expected from them. Greetings and thanks in advance! Yvan. ____________________________________________________________________________________ Catch up on fall's hot new shows on Yahoo! TV. Watch previews, get listings, and more! http://tv.yahoo.com/collections/3658 From MICHELLE.MCNEESE <@t> childrens.com Wed Sep 19 11:26:25 2007 From: MICHELLE.MCNEESE <@t> childrens.com (MICHELLE MCNEESE) Date: Wed Sep 19 11:26:44 2007 Subject: [Histonet] Automated microtomes Message-ID: <46F10761020000290000BAAD@CNET3.CHILDRENS.COM> Recently, we have discovered that our techs are having foot problems (such as tendinitis and muscle strain) that we believe to be associated with using the foot pedals on the automated microtomes (as well as the foot pedal on the embedding center). 1. Have any other facilities that use these types of instruments had problems or noticed a shift from hand/wrist problems to foot/ankle problems? 2. If so, what are your facilities doing to help alleviate these issues? Thank you in advance for helping guide us through some uncharted territory. Michelle L McNeese, HT (ASCP) Lead Tech, Histology Department of Pathology Childrens Medical Center (214) 456-6146 Email: michelle.mcneese@childrens.com From jnocito <@t> satx.rr.com Wed Sep 19 11:27:55 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Sep 19 11:28:06 2007 Subject: [Histonet] paraffin processing problem References: Message-ID: <009701c7fada$0a020eb0$0202a8c0@yourxhtr8hvc4p> this is just a guess, but I would check the paraffin lines and/or the levels Joe ----- Original Message ----- From: "Carla M Conway" To: Sent: Wednesday, September 19, 2007 10:54 AM Subject: [Histonet] paraffin processing problem > > Hello, > > I have an LX120 tissue processor. The paraffin infiltration time is > programmed for three 1hour stations. Sometime last night a problem > occurred during the paraffin cycle (unfortunately, we do not have a > printout or record of when it happened). This morning the Fill Failure > alarm was sounding and tissues were not soaking in paraffin as they should > have been. There was only paraffin residue in the processing chamber, so > I > know that the tissues were exposed to paraffin but don't know for how > long. > What should I do? I do not want to overprocess them in paraffin, but don't > want them underinfiltrated either. The tissues are staghorn sculpin hearts > and livers. > > Thanks so much for your help, > > > > Carla Conway > > Western Fisheries Research Center > 6505 NE 65th St. > Seattle, WA 98115 > cmconway@usgs.gov > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From slappycraw <@t> yahoo.com Wed Sep 19 11:37:19 2007 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Wed Sep 19 11:37:31 2007 Subject: [Histonet] paraffin processing problem In-Reply-To: <009701c7fada$0a020eb0$0202a8c0@yourxhtr8hvc4p> Message-ID: <692176.77544.qm@web53611.mail.re2.yahoo.com> Ditto Joe Nocito wrote: this is just a guess, but I would check the paraffin lines and/or the levels Joe ----- Original Message ----- From: "Carla M Conway" To: Sent: Wednesday, September 19, 2007 10:54 AM Subject: [Histonet] paraffin processing problem > > Hello, > > I have an LX120 tissue processor. The paraffin infiltration time is > programmed for three 1hour stations. Sometime last night a problem > occurred during the paraffin cycle (unfortunately, we do not have a > printout or record of when it happened). This morning the Fill Failure > alarm was sounding and tissues were not soaking in paraffin as they should > have been. There was only paraffin residue in the processing chamber, so > I > know that the tissues were exposed to paraffin but don't know for how > long. > What should I do? I do not want to overprocess them in paraffin, but don't > want them underinfiltrated either. The tissues are staghorn sculpin hearts > and livers. > > Thanks so much for your help, > > > > Carla Conway > > Western Fisheries Research Center > 6505 NE 65th St. > Seattle, WA 98115 > cmconway@usgs.gov > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Larry A. Woody Amgen Seattle, Wa. --------------------------------- Don't let your dream ride pass you by. Make it a reality with Yahoo! Autos. From rjbuesa <@t> yahoo.com Wed Sep 19 11:50:54 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 19 11:51:06 2007 Subject: [Histonet] Carpet in Histology In-Reply-To: <46F102E1.9B87.00AC.0@mercyhealth.com> Message-ID: <611521.20856.qm@web61221.mail.yahoo.com> Paraffin does NOT vacuum at all. You for sure will step on it and make it to stick to the floor. Floor with paraffin have to be scrapped. Carpets (removable) are the best solution for areas where paraffin can fall to the floor. We used to have a service that brough / took away the carpets weekly. Ren? J. Marcia Funk wrote: _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. From rjbuesa <@t> yahoo.com Wed Sep 19 11:54:07 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 19 11:54:20 2007 Subject: [Histonet] Automated microtomes In-Reply-To: <46F10761020000290000BAAD@CNET3.CHILDRENS.COM> Message-ID: <200921.50363.qm@web61218.mail.yahoo.com> You have to be realize that ANY and ALL repetitive motions will end with some type of tendonitis. The only solution is to rotate tasks, or take breaks, usually every half an hour, or do some sort of exercise. Ren? J. --------------------------------- Need a vacation? Get great deals to amazing places on Yahoo! Travel. From gcallis <@t> montana.edu Wed Sep 19 12:30:05 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed Sep 19 12:29:50 2007 Subject: [Histonet] Carpet in Histology In-Reply-To: <611521.20856.qm@web61221.mail.yahoo.com> References: <46F102E1.9B87.00AC.0@mercyhealth.com> <611521.20856.qm@web61221.mail.yahoo.com> Message-ID: <6.0.0.22.1.20070919111835.01b3eab0@gemini.msu.montana.edu> Rene and everyone, What we have are commercial area rugs (very low looped fibers, as used in foyers near doors of buildings entrances. Some are longer rug runners, and a bit heavy, but they vacuum very nicely as long as the vacuum is set to lowest setting and an industrial vacuum cleaner. It is not a 100% paraffin removal, but it takes up most of the trimmings. Our building maintenance people would never haul them off for cleaning. That has been done one time in 20 years!! Carpet throughout a whole laboratory would not be a good thing to have. We only use area rugs to contain paraffin at microtomy and embedding stations. If they become too trashed, we get rid of them and buy new. At 10:50 AM 9/19/2007, you wrote: >Paraffin does NOT vacuum at all. You for sure will step on it and make it >to stick to the floor. Floor with paraffin have to be scrapped. Carpets >(removable) are the best solution for areas where paraffin can fall to the >floor. We used to have a service that brough / took away the carpets weekly. > Ren? J. > >Marcia Funk wrote: > > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >--------------------------------- >Looking for a deal? Find great prices on flights and hotels with Yahoo! >FareChase. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 From emilietrudelle <@t> hotmail.com Wed Sep 19 13:09:02 2007 From: emilietrudelle <@t> hotmail.com (emilie trudelle) Date: Wed Sep 19 13:09:17 2007 Subject: [Histonet] NK cells Message-ID: Hi Histonetters, Has anyone succesfully labelled NK cells in frozen sections of C3H mouse tissues? If so, wich antibody did you used and where did you purchased it?????? I made my research and i only found Nk1.1 for IHC (wich I can't use because of our mouse strain) or antibody for flow cytometry.... Thanks Emilie Trudelle University of Montreal _________________________________________________________________ Soyez parmi les premiers ? essayer Windows Live Mail. http://ideas.live.com/programpage.aspx?versionId=5d21c51a-b161-4314-9b0e-4911fb2b2e6d From doug <@t> ppspath.com Wed Sep 19 14:17:33 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Wed Sep 19 13:18:14 2007 Subject: {SPAM?} [Histonet] What are those things called? In-Reply-To: <6.0.0.22.1.20070919095648.01b57908@gemini.msu.montana.edu> Message-ID: It was a HistoBath II from Thermo. It has been discontinued and no item will replace it. No reason was given why. Maybe it is something to do with too many companies merging into one and they couldn't fit the name on it? Beats me? :) I found a used one online that I am purchasing. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: Wednesday, September 19, 2007 10:57 AM To: Douglas D Deltour Subject: RE: {SPAM?} [Histonet] What are those things called? Give us the name of it and where you found it. Thanks At 07:54 AM 9/19/2007, you wrote: >Never mind I found it. I hate it when I do that. > >Douglas D. Deltour HT(ASCP) >Histology Manager >Professional Pathology Services, PC >One Science Court >Suite 200 >Columbia, SC 29203 >Office (803)252-1913 >Fax (803)254-3262 >Doug@ppspath.com >***************************************************** >PROFESSIONAL PATHOLOGY SERVICES, PC >NOTICE OF CONFIDENTIALITY >This message is intended only for the use of the individual or entity to >which it is addressed and may contain information that is privileged, >confidential and exempt from disclosure under applicable law. If the reader >of this message is not the intended recipient, you are hereby notified that >any dissemination, distribution, or copying of this communication is >strictly prohibited by law. If you have received this communication in >error, please notify me immediately. > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D >Deltour >Sent: Wednesday, September 19, 2007 8:29 AM >To: histonet@lists.utsouthwestern.edu >Subject: {SPAM?} [Histonet] What are those things called? > >I am looking for the "cold bath" or whatever they are called for frozen >sectioning. They are the things that you put the 2-methyl butane in to >freeze the molds. Any idea on who carries them? Thanks. > > > > > >Douglas D. Deltour HT(ASCP) > >Histology Manager > >Professional Pathology Services, PC > >One Science Court > >Suite 200 > >Columbia, SC 29203 > >Office (803)252-1913 > >Fax (803)254-3262 > >Doug@ppspath.com > >***************************************************** > >PROFESSIONAL PATHOLOGY SERVICES, PC >NOTICE OF CONFIDENTIALITY >This message is intended only for the use of the individual or entity to >which it is addressed and may contain information that is privileged, >confidential and exempt from disclosure under applicable law. If the reader >of this message is not the intended recipient, you are hereby notified that >any dissemination, distribution, or copying of this communication is >strictly prohibited by law. If you have received this communication in >error, please notify me immediately. > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From Jackie.O'Connor <@t> abbott.com Wed Sep 19 13:41:30 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed Sep 19 13:42:17 2007 Subject: {SPAM?} [Histonet] What are those things called? In-Reply-To: <60bv13$6l4r2o@viper.abbott.com> Message-ID: Are you maybe talking about the Dewars (like a thermos) you can use to contain liquid nitrogen etc?? Dewars are available through VWR. "Douglas D Deltour" Sent by: histonet-bounces@lists.utsouthwestern.edu 09/19/2007 02:17 PM To "'Gayle Callis'" , cc Subject RE: {SPAM?} [Histonet] What are those things called? It was a HistoBath II from Thermo. It has been discontinued and no item will replace it. No reason was given why. Maybe it is something to do with too many companies merging into one and they couldn't fit the name on it? Beats me? :) I found a used one online that I am purchasing. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: Wednesday, September 19, 2007 10:57 AM To: Douglas D Deltour Subject: RE: {SPAM?} [Histonet] What are those things called? Give us the name of it and where you found it. Thanks At 07:54 AM 9/19/2007, you wrote: >Never mind I found it. I hate it when I do that. > >Douglas D. Deltour HT(ASCP) >Histology Manager >Professional Pathology Services, PC >One Science Court >Suite 200 >Columbia, SC 29203 >Office (803)252-1913 >Fax (803)254-3262 >Doug@ppspath.com >***************************************************** >PROFESSIONAL PATHOLOGY SERVICES, PC >NOTICE OF CONFIDENTIALITY >This message is intended only for the use of the individual or entity to >which it is addressed and may contain information that is privileged, >confidential and exempt from disclosure under applicable law. If the reader >of this message is not the intended recipient, you are hereby notified that >any dissemination, distribution, or copying of this communication is >strictly prohibited by law. If you have received this communication in >error, please notify me immediately. > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D >Deltour >Sent: Wednesday, September 19, 2007 8:29 AM >To: histonet@lists.utsouthwestern.edu >Subject: {SPAM?} [Histonet] What are those things called? > >I am looking for the "cold bath" or whatever they are called for frozen >sectioning. They are the things that you put the 2-methyl butane in to >freeze the molds. Any idea on who carries them? Thanks. > > > > > >Douglas D. Deltour HT(ASCP) > >Histology Manager > >Professional Pathology Services, PC > >One Science Court > >Suite 200 > >Columbia, SC 29203 > >Office (803)252-1913 > >Fax (803)254-3262 > >Doug@ppspath.com > >***************************************************** > >PROFESSIONAL PATHOLOGY SERVICES, PC >NOTICE OF CONFIDENTIALITY >This message is intended only for the use of the individual or entity to >which it is addressed and may contain information that is privileged, >confidential and exempt from disclosure under applicable law. If the reader >of this message is not the intended recipient, you are hereby notified that >any dissemination, distribution, or copying of this communication is >strictly prohibited by law. If you have received this communication in >error, please notify me immediately. > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ngale <@t> bccancer.bc.ca Wed Sep 19 14:37:12 2007 From: Ngale <@t> bccancer.bc.ca (Gale, Nadia) Date: Wed Sep 19 14:37:24 2007 Subject: [Histonet] TUNEL Message-ID: Does anyone have a TUNEL assay developed for use with the Ventana Discovery XT that they are willing to share? I need to stain xenografts (human in mouse) and have not had much success thus far. Thanks, Nadia P.S. Thank you for the help with my mouse IHC problems--I've solved the background issue--it was due to interference from the secondary antibody in the detection kit. Nadia Gale Lead Histotechnologist Centre for Translational and Applied Genomics (CTAG) 3427-600 West 10th Avenue Vancouver, BC V5Z 4E6 tel 604-877-6000 ext 2426 fax 604-877-6089 ngale@bccancer.bc.ca From Nancy.Warren <@t> nkch.org Wed Sep 19 14:38:23 2007 From: Nancy.Warren <@t> nkch.org (Nancy Warren) Date: Wed Sep 19 14:38:50 2007 Subject: [Histonet] (no subject) Message-ID: <77FADC01535C0B4FAC7EC79961E9B73C5E6904@Belgian.nkch.org> Skippi ext. 5041 Be kind to unkind people (they probably need it the most). Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s), and may contain privileged or confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by replying to this email, and destroy all copies of the original message. From dw18 <@t> uchicago.edu Wed Sep 19 15:39:01 2007 From: dw18 <@t> uchicago.edu (David A. Wright) Date: Wed Sep 19 15:39:19 2007 Subject: [Histonet] Nikon Optiphot cube changing Message-ID: <20070919153901.ARB89310@m4500-03.uchicago.edu> Hi Histonet Can anyone help me with instructions for changing the fluorescence cubes in an old Nikon Optiphot? It has an EFD-3 (4 position) cube holder. We acquired the microscope from another investigator, without any manuals, and up to now have been using it purely for bright field conventional staining. Now I'd like to use it for fluorescence too. The installed cubes work fine but I'd like to add in a DAPI cube, borrowed from an old Labophot. I've changed cubes on several models of microscope but it's not obvious to me how to take out these EFD-3 modules. Needless to say, with this pre-infinity model microscope, it is no longer supported at the Nikon-USA website. If anyone could tell me how to do it, or link me to a pdf of the relevant manual(s), I'd be extremely grateful! Thanks! -David Wright University of Chicago, Neurosurgery From shive003 <@t> umn.edu Wed Sep 19 15:58:14 2007 From: shive003 <@t> umn.edu (Jan Shivers) Date: Wed Sep 19 15:58:27 2007 Subject: [Histonet] EM position Message-ID: <017801c7faff$cbcb95f0$a1065486@auxs.umn.edu> The University of Minnesota Veterinary Diagnostic Laboratory will soon have a position available in the Electron Microscopy Laboratory. This position will be a supervisory position, and essential applicant requirements will include knowledge and experience in negative staining and plastic-embedded transmission electron microscopy. Please contact me directly, if you're interested in more specifics. Thanks. Jan Shivers Section Head - IHC/Histo/EM UMN Veterinary Diagnostic Laboratory St. Paul, MN shive003@umn.edu From Bonnie.Heitzman <@t> baycare.org Wed Sep 19 16:06:09 2007 From: Bonnie.Heitzman <@t> baycare.org (Heitzman, Bonnie) Date: Wed Sep 19 16:07:15 2007 Subject: [Histonet] RE: Need your help to find a Cytology Supervisor Message-ID: <98ECE5B11C59DE449F8949A45C52A8190872D4A2@EXCHANGE02-VS.BCAD.BAYCARE.ORG> Try Cytopathnet.org as a resource/listserv for Cytology. Bonnie J Heitzman Pathology Services Manager bonnie.heitzman@baycare.org Confidential: This electronic message and all contents contain information from BayCare Health System which may be privileged, confidential or otherwise protected from disclosure. The information is intended to be for the addressee only. If you are not the addressee, any disclosure, copy, distribution or use of the contents of this message is prohibited. If you have received this electronic message in error, please notify the sender and destroy the original message and all copies. From jerry.santiago <@t> jax.ufl.edu Thu Sep 20 05:22:51 2007 From: jerry.santiago <@t> jax.ufl.edu (Santiago, Jerry) Date: Thu Sep 20 05:27:15 2007 Subject: [Histonet] Telomerase antibody Message-ID: <816DC61E1730E843A0BCF4CCFA1EFCF3065501@jaxmail.umc.ufl.edu> Anyone using the telomerase antibody with success? I need some help trying to get this antibody to perform well. Jerry Santiago, BS, HTL(ASCP)QIHC Shands Jacksonville Jacksonville, Florida E-mail: jerry.santiago@jax.ufl.edu From doakes <@t> olympicmedical.org Thu Sep 20 07:53:46 2007 From: doakes <@t> olympicmedical.org (Dawn Oakes) Date: Thu Sep 20 07:56:22 2007 Subject: [Histonet] Gram -/+ Message-ID: <7B6A91693097B34CBB897045D45E4E2B06A3F4FA@is-210s.olympicmedical.local> Does anyone out there use liquid saturated aqueous picric acid for the B & B stain and what ratio do you use to the acetone. Dawn Oakes Olympic Medical Center From amgomez <@t> mail.unomaha.edu Thu Sep 20 08:12:50 2007 From: amgomez <@t> mail.unomaha.edu (Adam M Gomez) Date: Thu Sep 20 08:14:01 2007 Subject: {SPAM?} Re: [Histonet] I think I will give it an acronym In-Reply-To: <1F937FB30BDB7C4A9F39F83FEA8D379F3E4C2D@bruexchange1.digestivespecialists.com> Message-ID: Please remove me from your distro box. V/R SSgt Gomez Adam Gomez NCOIC, Personnel AFROTC Det 470 Comm: 402-554-3402 Fax: 402-554-2999 University of Nebraska at Omaha amgomez@mail.unomaha.edu "Blazek, Linda" Sent by: histonet-bounces@lists.utsouthwestern.edu 09/18/2007 01:38 PM To "Carol Sidell" , "Douglas D Deltour" , "Cheryl R. Kerry" , "Emily Sours" , cc Subject RE: {SPAM?} Re: [Histonet] I think I will give it an acronym GO BROWNS!!!!!! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Sidell Sent: Tuesday, September 18, 2007 11:32 AM To: Douglas D Deltour; Cheryl R. Kerry; Emily Sours; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym I am a lifetime Cleveland Browns fan so I really don't like the Steelers either!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, September 18, 2007 9:20 AM To: 'Cheryl R. Kerry'; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym I am a lifetime Chargers fan so now you know why I hate the Steelers and the Patriots. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: Cheryl R. Kerry [mailto:tkngflght@yahoo.com] Sent: Tuesday, September 18, 2007 9:16 AM To: 'Douglas D Deltour'; 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym Oh Doug--you and I can no longer be friends!!! Born and raised a Steeler fan--in fact, if you don't say it 'STILLERS' you ain't a real fan!! (Go Emily!!) Don't even get me started on the Pirates and the Pens!! Cheryl Cheryl R. Kerry, HT(ASCP) Full Staff Inc. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, September 18, 2007 9:51 AM To: 'Emily Sours'; histonet@lists.utsouthwestern.edu Subject: RE: {SPAM?} Re: [Histonet] I think I will give it an acronym Did you have to mention football? Do you know how much I dislike Steelers fans? Almost as much as Patriot fans. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, September 18, 2007 8:35 AM To: histonet@lists.utsouthwestern.edu Subject: {SPAM?} Re: [Histonet] I think I will give it an acronym I'm all about ThermoFisher, since Fisher was started in Pittsburgh. go steelers! (I've been assimilated into the city, not being a true Pittsburgher.) I'll take your allyourbase reference and up it one chocolate rain: chocolate rain thermofisher has no sense of shame Emily -- Remember our war hysteria, when we called sauerkraut 'Liberty cabbage' and somebody actually proposed calling German measles, 'Liberty measles?'...Remember when the hick legislators in certain states, in obedience to William Jennings Bryan, who learned his biology from his pious old grandma, set up shop as scientific experts and made the whole world laugh itself sick by forbidding the teaching of evolution? --Sinclair Lewis, It Can't Happen Here, 1935 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lynn.Burton <@t> Illinois.gov Thu Sep 20 08:30:11 2007 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Thu Sep 20 08:32:34 2007 Subject: [Histonet] Gram -/+ References: <7B6A91693097B34CBB897045D45E4E2B06A3F4FA@is-210s.olympicmedical.local> Message-ID: I use .5 gm of picric acid dissolved in 900mL of acetone. Then I qs to 1000mL. Lynn Burton Animal Disease Lab ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Dawn Oakes Sent: Thu 9/20/2007 7:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gram -/+ Does anyone out there use liquid saturated aqueous picric acid for the B & B stain and what ratio do you use to the acetone. Dawn Oakes Olympic Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Thu Sep 20 08:40:42 2007 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Sep 20 08:41:34 2007 Subject: [Histonet] Telomerase antibody In-Reply-To: <816DC61E1730E843A0BCF4CCFA1EFCF3065501@jaxmail.umc.ufl.edu> References: <816DC61E1730E843A0BCF4CCFA1EFCF3065501@jaxmail.umc.ufl.edu> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB735C@EMAIL.archildrens.org> I have used this antibody. Dako retrieval, pressure cooker, 10 minutes, incubation 20 minutes, dilution 1:40. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Santiago, Jerry Sent: Thursday, September 20, 2007 5:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Telomerase antibody Anyone using the telomerase antibody with success? I need some help trying to get this antibody to perform well. Jerry Santiago, BS, HTL(ASCP)QIHC Shands Jacksonville Jacksonville, Florida E-mail: jerry.santiago@jax.ufl.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From TJJ <@t> Stowers-Institute.org Thu Sep 20 09:31:01 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Thu Sep 20 09:31:46 2007 Subject: [Histonet] NTMT woes Message-ID: We're really stumped on what's going on here. For years we have made this solution without any trouble or issue. Suddenly in the past several months, we're having trouble with it going cloudy one day after making it. I made some yesterday, and by this morning it was cloudy. I checked the pH and it was 9.3 so I'm not too worried that it's a pH issue. This cloudiness occurs regardless of whether we use Tris base and pH with HCl, or if we use Tris-HCl and pH with sodium hydroxide. Here is the recipe: NTMT (500 ml) NaCl 5M............................................10 ml TrisHCl, 2M, pH 9.5................................25 ml MgCl2 2M.........................................12.5 ml Tween-20 (10% in dd water).........................50 ml q.s. to 500 ml (store at RT) NaCl 5M (100 ml) NaCl (FW 58.44)....................29.22 g q.s. to 100 ml (store at RT) TrisHCl, 2M (500 ml) Tris Base (FW 121.1).............121.1 g Dissolve Tris base in 300 ml of d water. pH to 9.5 with HCl. q.s. to 500 ml (store at RT) MgCl2, 2M (100 ml) MgCl2 (FW 203.3)..................40.6 g q.s. to 100 ml (store at RT) If we omit the magnesium chloride, it stays clear. If we add it, by the next day it is very cloudy and cannot use the solution. Can anybody give me any information on what might be happening, and what we need to do? We have already replaced the magnesium chloride, thinking it had gone bad somehow. It's still happening. Thanks for any help you can give us. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From gcallis <@t> montana.edu Thu Sep 20 09:50:35 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Sep 20 09:50:54 2007 Subject: [Histonet] Floor in a pathology laboratory In-Reply-To: <011f01c7fac0$d6778390$0202a8c0@yourxhtr8hvc4p> References: <18E3095CC1A30E4684F55D6D757C8D2E51646A@phoex1.peacehealth.org> <413123.40754.qm@web61218.mail.yahoo.com> <6.0.0.22.1.20070918142638.01b34e08@gemini.msu.montana.edu> <011f01c7fac0$d6778390$0202a8c0@yourxhtr8hvc4p> Message-ID: <6.0.0.22.1.20070920084559.01b0fda0@gemini.msu.montana.edu> Our chairs roll over the commercial low nap (sp) area rugs without any problems. Maybe it was the chair wheels and not the area rug causing the problem. It pays to make sure the rugs are located in such a way that wheels stay on the rug surface at all times. These area rugs are very heavy duty designed for entryway use, not very pretty or glamorous, but they work fine for any work area involved with paraffin. We have never had these rugs "rise up" on us due to the weight of the rug itself. They have heavy black rubber backing and do not slip nor bunch up. We also use them in front of sinks. At 07:27 AM 9/19/2007, you wrote: >I had some carpets like this and is was a bear to work with in the >embedding area. The wheels of the chairs kept causing the carpet to rise >up, causing a tripping hazard. Anyone else experience this? By the >microtomes, the carpet worked fine. > >J Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From talulahgosh <@t> gmail.com Thu Sep 20 10:00:08 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu Sep 20 10:00:19 2007 Subject: [Histonet] NTMT woes In-Reply-To: References: Message-ID: We always make our NTMT the day we use it because the magnesium chloride precipitates sometimes. No idea why it precipitates sometimes and not others, maybe temperature of the room? Maybe the reagent precipitates as it gets older? We find it easier not to use a magnesium chloride stock solution--just add the MgCl2 directly to the final solution. Emily -- Remember our war hysteria, when we called sauerkraut 'Liberty cabbage' and somebody actually proposed calling German measles, 'Liberty measles?'...Remember when the hick legislators in certain states, in obedience to William Jennings Bryan, who learned his biology from his pious old grandma, set up shop as scientific experts and made the whole world laugh itself sick by forbidding the teaching of evolution? --Sinclair Lewis, It Can't Happen Here, 1935 From tkngflght <@t> yahoo.com Thu Sep 20 10:01:19 2007 From: tkngflght <@t> yahoo.com (Cheryl R. Kerry) Date: Thu Sep 20 10:01:27 2007 Subject: [Histonet] Carpet rental and chair wheels. In-Reply-To: <6.0.0.22.1.20070920084559.01b0fda0@gemini.msu.montana.edu> Message-ID: <00b001c7fb97$1a2515e0$6701a8c0@CHERYLSLAPTOP> Most of the uniform services will provide industrial rugs for a rental fee. They are heavy weight, won't buckle, and don't cause a problem with chair wheels. They can be switched out as you choose to schedule, and they create an increase in safety because the paraffin and liquid spills don't land on linoleum. A couple of phone calls and the uniform people will even bring samples and sometimes you can even pick your color!! There are different chair wheels for hard surfaces vs. carpet--you can purchase these from most office supply companies and they fit most chairs so the switch is tool-less (easy). Hope this helps! Signed--a former 'safety princess' for a large national lab. :) Cheryl R. Kerry, HT(ASCP) Full Staff Inc. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Thursday, September 20, 2007 9:51 AM To: Joe Nocito; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Floor in a pathology laboratory Our chairs roll over the commercial low nap (sp) area rugs without any problems. Maybe it was the chair wheels and not the area rug causing the problem. It pays to make sure the rugs are located in such a way that wheels stay on the rug surface at all times. These area rugs are very heavy duty designed for entryway use, not very pretty or glamorous, but they work fine for any work area involved with paraffin. We have never had these rugs "rise up" on us due to the weight of the rug itself. They have heavy black rubber backing and do not slip nor bunch up. We also use them in front of sinks. At 07:27 AM 9/19/2007, you wrote: >I had some carpets like this and is was a bear to work with in the >embedding area. The wheels of the chairs kept causing the carpet to rise >up, causing a tripping hazard. Anyone else experience this? By the >microtomes, the carpet worked fine. > >J Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rich.strauss <@t> Esoterix.com Thu Sep 20 10:01:46 2007 From: rich.strauss <@t> Esoterix.com (Strauss, Rich) Date: Thu Sep 20 10:01:57 2007 Subject: [Histonet] unsubscribe... Message-ID: Richard Strauss HT, QIHC IHC/HISTOLOGY SUPERVISOR US LABS BRENTWOOD 615 377-7151 From cervantes <@t> bendres.com Thu Sep 20 10:04:31 2007 From: cervantes <@t> bendres.com (Cervantes, Jessica) Date: Thu Sep 20 10:04:46 2007 Subject: [Histonet] EM position In-Reply-To: <017801c7faff$cbcb95f0$a1065486@auxs.umn.edu> References: <017801c7faff$cbcb95f0$a1065486@auxs.umn.edu> Message-ID: <8943D65F9AD70E4488AD6DE09F15088768C75F@BRIEX04A> Jan - You might want to post this to the Microscopy ListServer, sponsored by Microscopy Society of America. http://www.microscopy.com/MicroscopyListserver ____________________ Jessica Cervantes Bend Research Inc -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jan Shivers Sent: Wednesday, September 19, 2007 1:58 PM To: histonet Subject: [Histonet] EM position The University of Minnesota Veterinary Diagnostic Laboratory will soon have a position available in the Electron Microscopy Laboratory. This position will be a supervisory position, and essential applicant requirements will include knowledge and experience in negative staining and plastic-embedded transmission electron microscopy. Please contact me directly, if you're interested in more specifics. Thanks. Jan Shivers Section Head - IHC/Histo/EM UMN Veterinary Diagnostic Laboratory St. Paul, MN shive003@umn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Thu Sep 20 10:21:13 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Thu Sep 20 10:21:31 2007 Subject: [Histonet] NTMT woes Message-ID: The precipitate must surely be magnesium hydroxide, as a consequence of the pH being too high. Precipitation is to be expected in an alkaline solution containing magnesium ions Data in the Merck Index indicate that Mg(OH)2 becomes insoluble at pH 9.5, which is close to the pH 9.3 of your solution. A small error in pH measurement, due to deterioration of the meter's electrode or of one of the buffers used for calibration, might result in a solution that is too alkaline. Curiousity. What does NTMT stand for? Google reveals this solution as an unexplained rinse to follow in situ hybridization, prior to what might be a histochemical reaction for an alkaline phosphatase label. Strongly alkaline rinses do not figure in the techniques given in the RMS In Situ Hybridization Handbook (Leitch et al, 1994). John Kiernan Anatomy, UWO London, Canada --- ----- Original Message ----- From: "Johnson, Teri" Date: Thursday, September 20, 2007 10:33 Subject: [Histonet] NTMT woes To: histonet@lists.utsouthwestern.edu > We're really stumped on what's going on here. For years we have made > this solution without any trouble or issue. Suddenly in the past > severalmonths, we're having trouble with it going cloudy one day > after making > it. I made some yesterday, and by this morning it was cloudy. I > checkedthe pH and it was 9.3 so I'm not too worried that it's a > pH issue. This > cloudiness occurs regardless of whether we use Tris base and pH with > HCl, or if we use Tris-HCl and pH with sodium hydroxide. > > Here is the recipe: > > NTMT (500 ml) > NaCl 5M............................................10 ml > TrisHCl, 2M, pH 9.5................................25 ml > MgCl2 2M.........................................12.5 ml > Tween-20 (10% in dd water).........................50 ml > q.s. to 500 ml (store at RT) > > > NaCl 5M (100 ml) > NaCl (FW 58.44)....................29.22 g > q.s. to 100 ml (store at RT) > > TrisHCl, 2M (500 ml) > Tris Base (FW 121.1).............121.1 g > Dissolve Tris base in 300 ml of d water. pH to 9.5 with HCl. > q.s. to 500 ml (store at RT) > > MgCl2, 2M (100 ml) > MgCl2 (FW 203.3)..................40.6 g > q.s. to 100 ml (store at RT) > > If we omit the magnesium chloride, it stays clear. If we add it, > by the > next day it is very cloudy and cannot use the solution. > > Can anybody give me any information on what might be happening, > and what > we need to do? We have already replaced the magnesium chloride, > thinkingit had gone bad somehow. It's still happening. > > Thanks for any help you can give us. > > Teri Johnson, HT(ASCP)QIHC > Managing Director Histology Facility > Stowers Institute for Medical Research > 1000 E. 50th St. > Kansas City, MO 64110 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From DennisH <@t> cookchildrens.org Thu Sep 20 10:21:11 2007 From: DennisH <@t> cookchildrens.org (Dennis Hahn) Date: Thu Sep 20 10:21:44 2007 Subject: [Histonet] Gram -/+ Message-ID: We order it premade from Poly Scientific, Cat #s1876. Dennis Dennis Hahn, HT (ASCP) Histology Lab Supervisor Cook Children's Medical Center 801 7th Avenue Ft Worth, Tx 76104-2796 682-885-6168 dennish@cookchildrens.org >>> "Dawn Oakes" 9/20/2007 7:53 AM >>> Does anyone out there use liquid saturated aqueous picric acid for the B & B stain and what ratio do you use to the acetone. Dawn Oakes Olympic Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------------------------------------ Cook Children's Health Care System This e-mail, facsimile, or letter and any files or attachments transmitted may contain information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Cook Children's Health Care System immediately at (682)885-4000 or via e-mail at compliance@cookchildrens.org. Cook Children's Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. ----------------------------------------------------------------------------------------------------------- From jcarpenter764 <@t> aol.com Thu Sep 20 10:21:40 2007 From: jcarpenter764 <@t> aol.com (jcarpenter764@aol.com) Date: Thu Sep 20 10:22:08 2007 Subject: [Histonet] test Message-ID: <8C9C9B113ECC4F8-DDC-5D47@WEBMAIL-DB13.sysops.aol.com> ________________________________________________________________________ Email and AIM finally together. You've gotta check out free AOL Mail! - http://mail.aol.com From jcarpenter764 <@t> aol.com Thu Sep 20 10:22:59 2007 From: jcarpenter764 <@t> aol.com (jcarpenter764@aol.com) Date: Thu Sep 20 10:23:22 2007 Subject: [Histonet] test Message-ID: <8C9C9B1436244E8-DDC-5D67@WEBMAIL-DB13.sysops.aol.com> ________________________________________________________________________ Email and AIM finally together. You've gotta check out free AOL Mail! - http://mail.aol.com From godsgalnow <@t> aol.com Thu Sep 20 10:25:12 2007 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Thu Sep 20 10:25:32 2007 Subject: [Histonet] FISH Message-ID: <8C9C9B19252389E-A0C-5BCD@MBLK-M09.sysops.aol.com> Can anybody that is doing FISH help me out by answering the following questions? What FISH testing do you currently do? DO you plan on adding any other FISH testing? What is the volume of FISH that you do? Daily?? Weekly? Is any part of it automated?? How? Who does the prep work for the FISH ? Pretreatment?? Hydridization? Post-treatment?? How many staff members do this part of the process? Who does the enumeration? Cytotechs?? Pathologists?? Histotechs?? How many staff members do this part of the process? How many cells do you count? Are the staff members that are doing FISH doing only FISH? ANy other information that you can provide would be extremely helpful. Thanks, Roxanne ________________________________________________________________________ Email and AIM finally together. You've gotta check out free AOL Mail! - http://mail.aol.com From HoustonR <@t> chi.osu.edu Thu Sep 20 10:28:52 2007 From: HoustonR <@t> chi.osu.edu (Houston, Ronald) Date: Thu Sep 20 10:29:32 2007 Subject: [Histonet] glass knife maker Message-ID: <979FF5962E234F45B06CF0DB7C1AABB2121FB066@chi2k3ms01.columbuschildrens.net> Does anyone know where I can get my hands on a reconditioned or unused glass knife maker? Thanks Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Columbus Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 houstonr@chi.osu.edu ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From asachau <@t> titanmed.com Thu Sep 20 10:32:19 2007 From: asachau <@t> titanmed.com (April Sachau) Date: Thu Sep 20 10:34:32 2007 Subject: [Histonet] glass knife maker In-Reply-To: <979FF5962E234F45B06CF0DB7C1AABB2121FB066@chi2k3ms01.columbuschildrens.net> Message-ID: <7E3ACD48BA6E26408F3188FBF08693F7C60C9C@titansbs1.corp.titanmed.com> http://www.labx.com/v2/newad.cfm?catID=125 site for used and new products... worth checking out. Hope it helps :) April Sachau Titan Medical Group Staff Supervisor Phone (866) 332-9600 Ext. 1023 Fax (402) 332-5181 asachau@titanmed.com see us on the web at www.titanmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Thursday, September 20, 2007 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] glass knife maker Does anyone know where I can get my hands on a reconditioned or unused glass knife maker? Thanks Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Columbus Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 houstonr@chi.osu.edu ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJJ <@t> Stowers-Institute.org Thu Sep 20 10:38:40 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Thu Sep 20 10:39:18 2007 Subject: [Histonet] NTMT woes In-Reply-To: Message-ID: Good point, John. I will have my pH verified by our media prep lab. NTMT are the first initials in the materials used to make it (NaCl + Tris-HCl + Magnesium chloride + Tween). We are using it as not only buffer rinses prior to chromogen substrate incubation, but also as the diluent for the NBT (Nitro blue tetrazolium chloride; C40H30Cl2N10O6; MW 817.7) and BCIP (5-Bromo-4-chloro-3-indolyl phosphate, toluidine salt; C8H6NO4BrCIP X C7H9N; MW 433.6). I will also be reordering the Magnesium chloride in a smaller amount - I'm wondering if ours is too saturated with water already (one month old). Teri -----Original Message----- From: John Kiernan [mailto:jkiernan@uwo.ca] Sent: Thursday, September 20, 2007 10:21 AM To: histonet@lists.utsouthwestern.edu; Johnson, Teri Subject: Re: [Histonet] NTMT woes The precipitate must surely be magnesium hydroxide, as a consequence of the pH being too high. Precipitation is to be expected in an alkaline solution containing magnesium ions Data in the Merck Index indicate that Mg(OH)2 becomes insoluble at pH 9.5, which is close to the pH 9.3 of your solution. A small error in pH measurement, due to deterioration of the meter's electrode or of one of the buffers used for calibration, might result in a solution that is too alkaline. Curiousity. What does NTMT stand for? Google reveals this solution as an unexplained rinse to follow in situ hybridization, prior to what might be a histochemical reaction for an alkaline phosphatase label. Strongly alkaline rinses do not figure in the techniques given in the RMS In Situ Hybridization Handbook (Leitch et al, 1994). John Kiernan Anatomy, UWO London, Canada --- ----- Original Message ----- From: "Johnson, Teri" Date: Thursday, September 20, 2007 10:33 Subject: [Histonet] NTMT woes To: histonet@lists.utsouthwestern.edu > We're really stumped on what's going on here. For years we have made > this solution without any trouble or issue. Suddenly in the past > severalmonths, we're having trouble with it going cloudy one day > after making > it. I made some yesterday, and by this morning it was cloudy. I > checkedthe pH and it was 9.3 so I'm not too worried that it's a > pH issue. This > cloudiness occurs regardless of whether we use Tris base and pH with > HCl, or if we use Tris-HCl and pH with sodium hydroxide. > > Here is the recipe: > > NTMT (500 ml) > NaCl 5M............................................10 ml > TrisHCl, 2M, pH 9.5................................25 ml > MgCl2 2M.........................................12.5 ml > Tween-20 (10% in dd water).........................50 ml > q.s. to 500 ml (store at RT) > > > NaCl 5M (100 ml) > NaCl (FW 58.44)....................29.22 g > q.s. to 100 ml (store at RT) > > TrisHCl, 2M (500 ml) > Tris Base (FW 121.1).............121.1 g > Dissolve Tris base in 300 ml of d water. pH to 9.5 with HCl. > q.s. to 500 ml (store at RT) > > MgCl2, 2M (100 ml) > MgCl2 (FW 203.3)..................40.6 g > q.s. to 100 ml (store at RT) > > If we omit the magnesium chloride, it stays clear. If we add it, > by the > next day it is very cloudy and cannot use the solution. > > Can anybody give me any information on what might be happening, > and what > we need to do? We have already replaced the magnesium chloride, > thinkingit had gone bad somehow. It's still happening. > > Thanks for any help you can give us. > > Teri Johnson, HT(ASCP)QIHC > Managing Director Histology Facility > Stowers Institute for Medical Research > 1000 E. 50th St. > Kansas City, MO 64110 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jluis.palazon <@t> icman.csic.es Thu Sep 20 10:43:21 2007 From: jluis.palazon <@t> icman.csic.es (Jose Luis Palazon Fernandez) Date: Thu Sep 20 10:43:36 2007 Subject: [Histonet] (no subject) Message-ID: <16801432.86261190303001423.JavaMail.tomcat@potter> Dear histonet members I am a PhD studing from Venezuela. I would appreciate your opinion about the structure in the attached file. I think it could be a cross section of a taste bud but I am not sure. The section belongs to a "barbel" of a fish species I am studying. Thanks in advance. Jos? Luis Universidad de Oriente-Isla Margarita-Venezuela actualmente en: Instituto de Ciencias Marinas de Andalucia Puerto Real, C?diz, Espa?a. email: jluis.palazon@icman.csic.es From gcallis <@t> montana.edu Thu Sep 20 10:44:20 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Sep 20 10:44:38 2007 Subject: [Histonet] glass knife maker In-Reply-To: <979FF5962E234F45B06CF0DB7C1AABB2121FB066@chi2k3ms01.columb uschildrens.net> References: <979FF5962E234F45B06CF0DB7C1AABB2121FB066@chi2k3ms01.columbuschildrens.net> Message-ID: <6.0.0.22.1.20070920094342.01b18b78@gemini.msu.montana.edu> Triangular or Ralph knives? Try IMEB At 09:28 AM 9/20/2007, you wrote: >Does anyone know where I can get my hands on a reconditioned or unused >glass knife maker? >Thanks > >Ronnie Houston, MS, HT(ASCP)QIHC >Anatomic Pathology Manager >Columbus Children's Hospital >700 Children's Drive >Columbus, OH 43205 >(614) 722 5465 >houstonr@chi.osu.edu > > > > > >----------------------------------------- Confidentiality Notice: >The following mail message, including any attachments, is for the >sole use of the intended recipient(s) and may contain confidential >and privileged information. The recipient is responsible to >maintain the confidentiality of this information and to use the >information only for authorized purposes. If you are not the >intended recipient (or authorized to receive information for the >intended recipient), you are hereby notified that any review, use, >disclosure, distribution, copying, printing, or action taken in >reliance on the contents of this e-mail is strictly prohibited. If >you have received this communication in error, please notify us >immediately by reply e-mail and destroy all copies of the original >message. Thank you. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jluis.palazon <@t> icman.csic.es Thu Sep 20 10:52:28 2007 From: jluis.palazon <@t> icman.csic.es (Jose Luis Palazon Fernandez) Date: Thu Sep 20 10:54:46 2007 Subject: [Histonet] (no subject) Message-ID: <6360724.86381190303548213.JavaMail.tomcat@potter> Dear list-members How can I post a picture to the list? I sended one with my previous email but it did not appear as an attached file. thanks in advance Jose Luis Universidad de Oriente-Isla Margarita-Venezuela actualmente en: Instituto de Ciencias Marinas de Andalucia Puerto Real, C?diz, Espa?a. email: jluis.palazon@icman.csic.es From sbreeden <@t> nmda.nmsu.edu Thu Sep 20 11:14:35 2007 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Sep 20 11:14:48 2007 Subject: [Histonet] OT: Smile Message-ID: <4D14F0FC9316DD41972D5F03C070908B8F46B8@nmdamailsvr.nmda.ad.nmsu.edu> Can you imagine that the business cards for the Thermo/Fisher/et al group are now 3x5" cards? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From Beth.Fye <@t> HCAhealthcare.com Thu Sep 20 11:14:52 2007 From: Beth.Fye <@t> HCAhealthcare.com (Fye Beth) Date: Thu Sep 20 11:15:08 2007 Subject: [Histonet] Formaldehyde - Carcinogen Message-ID: <634870CD949E454381124308D16AB61955C252@NASEV05.hca.corpad.net> In 2006, the IARC change formaldehyde from a Group 2a - Probable Carcinogen to a Group I Cancinogenic to Humans. Has this changed where you are storing your formaldehyde, and any other procedures with in the Histology Laboratory? Our previous policy stated that carcinogens must be stored in secure cabinets however with the volume of formaldehyde in our labortory, that poses a problem. We currently have it stored on open shelving. Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 pager: (804)759-6929 From rjbuesa <@t> yahoo.com Thu Sep 20 11:21:02 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 20 11:21:16 2007 Subject: [Histonet] Gram -/+ In-Reply-To: <7B6A91693097B34CBB897045D45E4E2B06A3F4FA@is-210s.olympicmedical.local> Message-ID: <807390.1158.qm@web61223.mail.yahoo.com> I use 2 grams of aq.saturated picric acid + 100 mL of acetone Ren? J. Dawn Oakes wrote: Does anyone out there use liquid saturated aqueous picric acid for the B & B stain and what ratio do you use to the acetone. Dawn Oakes Olympic Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Moody friends. Drama queens. Your life? Nope! - their life, your story. Play Sims Stories at Yahoo! Games. From Lynn.Burton <@t> Illinois.gov Thu Sep 20 11:21:57 2007 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Thu Sep 20 11:23:08 2007 Subject: [Histonet] Formaldehyde - Carcinogen References: <634870CD949E454381124308D16AB61955C252@NASEV05.hca.corpad.net> Message-ID: We have not changed anything. Lynn Burton Animal Disease Lab ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Fye Beth Sent: Thu 9/20/2007 11:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formaldehyde - Carcinogen In 2006, the IARC change formaldehyde from a Group 2a - Probable Carcinogen to a Group I Cancinogenic to Humans. Has this changed where you are storing your formaldehyde, and any other procedures with in the Histology Laboratory? Our previous policy stated that carcinogens must be stored in secure cabinets however with the volume of formaldehyde in our labortory, that poses a problem. We currently have it stored on open shelving. Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 pager: (804)759-6929 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Sep 20 11:24:48 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 20 11:25:00 2007 Subject: [Histonet] (no subject) In-Reply-To: <6360724.86381190303548213.JavaMail.tomcat@potter> Message-ID: <456714.83682.qm@web61224.mail.yahoo.com> The instructions are in the web-site home page. Ren? J. Jose Luis Palazon Fernandez wrote: Dear list-members How can I post a picture to the list? I sended one with my previous email but it did not appear as an attached file. thanks in advance Jose Luis Universidad de Oriente-Isla Margarita-Venezuela actualmente en: Instituto de Ciencias Marinas de Andalucia Puerto Real, C?diz, Espa?a. email: jluis.palazon@icman.csic.es _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Don't let your dream ride pass you by. Make it a reality with Yahoo! Autos. From mpence <@t> grhs.net Thu Sep 20 11:28:18 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Sep 20 11:28:36 2007 Subject: [Histonet] Formaldehyde - Carcinogen In-Reply-To: <634870CD949E454381124308D16AB61955C252@NASEV05.hca.corpad.net> Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C729@IS-E2K3.grhs.net> Is it just formaldehyde or anything containing formaldehyde, like 10% NB formalin? Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fye Beth Sent: Thursday, September 20, 2007 11:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formaldehyde - Carcinogen In 2006, the IARC change formaldehyde from a Group 2a - Probable Carcinogen to a Group I Cancinogenic to Humans. Has this changed where you are storing your formaldehyde, and any other procedures with in the Histology Laboratory? Our previous policy stated that carcinogens must be stored in secure cabinets however with the volume of formaldehyde in our labortory, that poses a problem. We currently have it stored on open shelving. Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 pager: (804)759-6929 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Thu Sep 20 11:32:29 2007 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Sep 20 11:30:48 2007 Subject: [Histonet] OT: Smile In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B8F46B8@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B8F46B8@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <1F937FB30BDB7C4A9F39F83FEA8D379F3E4C34@bruexchange1.digestivespecialists.com> With teeny tiny print. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, September 20, 2007 12:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] OT: Smile Can you imagine that the business cards for the Thermo/Fisher/et al group are now 3x5" cards? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Thu Sep 20 11:40:30 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Sep 20 11:40:44 2007 Subject: [Histonet] PBS/MgCl2 Message-ID: <001401c7fba4$f5399b30$6412a8c0@dielangs.at> Hi FISHers, I am quite new in FISH-techniques. I have a protocol for imprint-preparation, where PBS/MgCl2 follows the pepsin digestionstep. Can anybody tell me, why I have to use PBS with MgCl2 to stop digestion? Isn't it the same, to use 1xPBS alone? Further there are different recipes for the solution. (5% 1M-MgCl2 in 1x PBS; 1000ml 1xPBS with 1,015 g MgCl2) That means one is 10fold richer on MgCls than the other. What pH is the right one here? Many questions, hot brain, help appreciated.. Gudrun From gentras <@t> vetmed.auburn.edu Thu Sep 20 12:05:10 2007 From: gentras <@t> vetmed.auburn.edu (Atoska Gentry) Date: Thu Sep 20 12:05:26 2007 Subject: [Histonet] PFA & paraffin Message-ID: <46F2A846.4080004@vetmed.auburn.edu> hello, does anyone have a tried & proven method for obtaining wrinkle free 6u cat brain sections that have been fixed in 4% PFA/0.1MPB , processed via standard for paraffin embedded sections? I briefly searched the histonet archive but after going thru approximately( x50)of 348 entries I figured there must be an easier/quicker way. Your prompt replies will be much appreciated. Atoska :-\ -- Atoska S. Gentry, B.S., HT(ASCP) Research Assistant IV Scott-Ritchey RSCH Center College of Vet. Med Auburn, AL 36849 PH (334) 844-5579 FAX (334) 844-5850 email: gentras@vetmed.auburn.edu From rjbuesa <@t> yahoo.com Thu Sep 20 12:19:53 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 20 12:20:13 2007 Subject: [Histonet] PFA & paraffin In-Reply-To: <46F2A846.4080004@vetmed.auburn.edu> Message-ID: <749490.55933.qm@web61214.mail.yahoo.com> Try adding a few drops of liquid detergent to the water bath. Ren? J. Atoska Gentry wrote: hello, does anyone have a tried & proven method for obtaining wrinkle free 6u cat brain sections that have been fixed in 4% PFA/0.1MPB , processed via standard for paraffin embedded sections? I briefly searched the histonet archive but after going thru approximately( x50)of 348 entries I figured there must be an easier/quicker way. Your prompt replies will be much appreciated. Atoska :-\ -- Atoska S. Gentry, B.S., HT(ASCP) Research Assistant IV Scott-Ritchey RSCH Center College of Vet. Med Auburn, AL 36849 PH (334) 844-5579 FAX (334) 844-5850 email: gentras@vetmed.auburn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Got a little couch potato? Check out fun summer activities for kids. From Beth.Fye <@t> HCAhealthcare.com Thu Sep 20 12:51:45 2007 From: Beth.Fye <@t> HCAhealthcare.com (Fye Beth) Date: Thu Sep 20 12:52:01 2007 Subject: [Histonet] Formaldehyde - Carcinogen In-Reply-To: <661949901A768E4F9CC16D8AF8F2838CA1C729@IS-E2K3.grhs.net> References: <634870CD949E454381124308D16AB61955C252@NASEV05.hca.corpad.net> <661949901A768E4F9CC16D8AF8F2838CA1C729@IS-E2K3.grhs.net> Message-ID: <634870CD949E454381124308D16AB61955C329@NASEV05.hca.corpad.net> >From what I have read, it appears to be any concentration including formalin. Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 pager: (804)759-6929 -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Thursday, September 20, 2007 12:28 PM To: Fye Beth; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Formaldehyde - Carcinogen Is it just formaldehyde or anything containing formaldehyde, like 10% NB formalin? Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fye Beth Sent: Thursday, September 20, 2007 11:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formaldehyde - Carcinogen In 2006, the IARC change formaldehyde from a Group 2a - Probable Carcinogen to a Group I Cancinogenic to Humans. Has this changed where you are storing your formaldehyde, and any other procedures with in the Histology Laboratory? Our previous policy stated that carcinogens must be stored in secure cabinets however with the volume of formaldehyde in our labortory, that poses a problem. We currently have it stored on open shelving. Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 pager: (804)759-6929 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From oscarmgonzalez <@t> hotmail.com Thu Sep 20 13:22:18 2007 From: oscarmgonzalez <@t> hotmail.com (Oscar M. Gonzalez) Date: Thu Sep 20 13:22:38 2007 Subject: [Histonet] CAP Question Message-ID: There is a new question in the CAP check list for this year, and I believe it refers to pathologist assistants: The question is ANP.11665:Are there written procedures for processing specimens? Does this mean that a separate procedure manual for grossing is necessary?. We used to get by with the next question, ANP.11670 about guidelines and instructions, but it seems like not any more. Thanks. _________________________________________________________________ More photos; more messages; more whatever ? Get MORE with Windows Live? Hotmail?. NOW with 5GB storage. http://imagine-windowslive.com/hotmail/?locale=en-us&ocid=TXT_TAGHM_migration_HM_mini_5G_0907 From MadaryJ <@t> MedImmune.com Thu Sep 20 13:49:04 2007 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Thu Sep 20 13:49:44 2007 Subject: [Histonet] Dontation/Sell CITADEL 1000 Message-ID: <8F3E1865E343C943BB38506D56FF0151010AF2F3@MD1EV002.medimmune.com> I have Citadel 1000 that has been in use since 2005, and it works perfectly! I am willing to donate to a non-profit organization, sell or exchange for other histology laboratory equipment. Please call Kendra Carter @ 301-398-4956 or e-mail at CarterK@medimmune.com Thank you. From Jessica.Vacca <@t> HCAhealthcare.com Thu Sep 20 13:59:26 2007 From: Jessica.Vacca <@t> HCAhealthcare.com (Vacca Jessica) Date: Thu Sep 20 14:01:26 2007 Subject: [Histonet] Formaldehyde - Carcinogen In-Reply-To: References: <634870CD949E454381124308D16AB61955C252@NASEV05.hca.corpad.net> Message-ID: <41E16A15CE78374EA45B57E0F94339B802CC778C@ORLEV01.hca.corpad.net> We keep formalin cubes in the morgue. Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Dr. Brandon Fl 33511 (813) 571-5193 or (813) 681-5551 ext 2454 Jessica.Vacca@hcahealthcare.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Burton, Lynn Sent: Thursday, September 20, 2007 12:22 PM To: Fye Beth; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Formaldehyde - Carcinogen We have not changed anything. Lynn Burton Animal Disease Lab ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Fye Beth Sent: Thu 9/20/2007 11:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formaldehyde - Carcinogen In 2006, the IARC change formaldehyde from a Group 2a - Probable Carcinogen to a Group I Cancinogenic to Humans. Has this changed where you are storing your formaldehyde, and any other procedures with in the Histology Laboratory? Our previous policy stated that carcinogens must be stored in secure cabinets however with the volume of formaldehyde in our labortory, that poses a problem. We currently have it stored on open shelving. Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 pager: (804)759-6929 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amgomez <@t> mail.unomaha.edu Thu Sep 20 15:22:15 2007 From: amgomez <@t> mail.unomaha.edu (Adam M Gomez) Date: Thu Sep 20 15:22:37 2007 Subject: [Histonet] Formaldehyde - Carcinogen In-Reply-To: <661949901A768E4F9CC16D8AF8F2838CA1C729@IS-E2K3.grhs.net> Message-ID: Please remove me from your list. V/R Adam Gomez NCOIC, Personnel AFROTC Det 470 Comm: 402-554-3402 Fax: 402-554-2999 University of Nebraska at Omaha amgomez@mail.unomaha.edu "Mike Pence" Sent by: histonet-bounces@lists.utsouthwestern.edu 09/20/2007 11:32 AM To "Fye Beth" , cc Subject RE: [Histonet] Formaldehyde - Carcinogen Is it just formaldehyde or anything containing formaldehyde, like 10% NB formalin? Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fye Beth Sent: Thursday, September 20, 2007 11:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formaldehyde - Carcinogen In 2006, the IARC change formaldehyde from a Group 2a - Probable Carcinogen to a Group I Cancinogenic to Humans. Has this changed where you are storing your formaldehyde, and any other procedures with in the Histology Laboratory? Our previous policy stated that carcinogens must be stored in secure cabinets however with the volume of formaldehyde in our labortory, that poses a problem. We currently have it stored on open shelving. Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 pager: (804)759-6929 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rchiovetti <@t> yahoo.com Thu Sep 20 15:28:06 2007 From: rchiovetti <@t> yahoo.com (Robert Chiovetti) Date: Thu Sep 20 15:28:22 2007 Subject: [Histonet] glass knife maker Message-ID: <744031.85511.qm@web58901.mail.re1.yahoo.com> Ronnie, I don't know what's in their used/rebuilt inventory these days, but try calling RMC Products, a part of Boeckeler Instruments, at: 800.552.2262 (toll-free in the USA). Cheers, Bob Robert (Bob) Chiovetti, Ph.D. Southwest Precision Instruments See What's New on Our Website! (www.swpinet.com) Arizona's Microscopy Resource 132 North Elster Drive Tucson, AZ 85710-3212 Tel./Fax 520-546-4986 Member, Arizona Small Business Association (www.asba.com) ----- Original Message ---- From: "Houston, Ronald" To: histonet@lists.utsouthwestern.edu Sent: Thursday, September 20, 2007 8:28:52 AM Subject: [Histonet] glass knife maker Does anyone know where I can get my hands on a reconditioned or unused glass knife maker? Thanks Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Columbus Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 houstonr@chi.osu.edu ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Pinpoint customers who are looking for what you sell. http://searchmarketing.yahoo.com/ From micro <@t> superlink.net Thu Sep 20 19:43:39 2007 From: micro <@t> superlink.net (Markus F. Meyenhofer) Date: Thu Sep 20 19:44:14 2007 Subject: [Histonet] glass knife maker References: <979FF5962E234F45B06CF0DB7C1AABB2121FB066@chi2k3ms01.columbuschildrens.net> Message-ID: <057901c7fbe8$74d63bd0$76893cd1@DJ4VDH31> We sell reconditioned LKB Knife Makers and Histo Knife Makers with guarantee. We also service your old one. Please contact me off Histonet. Regards, Markus F. Meyenhofer Microscopy Labs Box 338 61 West Street Red Bank, NJ 07701 732 747 6228 fax 732 758 9142 micro@superlink.net ----- Original Message ----- From: "Houston, Ronald" To: Sent: Thursday, September 20, 2007 11:28 AM Subject: [Histonet] glass knife maker Does anyone know where I can get my hands on a reconditioned or unused glass knife maker? Thanks Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Columbus Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 houstonr@chi.osu.edu ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Thu Sep 20 22:16:24 2007 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Thu Sep 20 22:16:39 2007 Subject: [Histonet] Re: Formaldehyde - Carcinogen? Message-ID: Beth A. Fye at HCA Richmond [Virginia?] Hospital Laboratories asks: >>In 2006, the IARC [International Agency for Research on Cancer] changed formaldehyde from a Group 2a - Probable Carcinogen to a Group I Carcinogenic to Humans. Has this changed... any procedures within [your] Histology Laboratory?<< See http://www.iarc.fr/ENG/Press_Releases/archives/pr153a.html I never heard of them, but I don't think the IARC is a regulatory agency - rather they're a subsidiary of the WHO (World Health Organization) based in France. Pathologists (at least this pathologist) have a very high lifetime exposure to formaldehyde. The AMA (American Medical Association) tracks the causes of death of American physicians very carefully. To date there has been no information published to suggest that pathologists' causes of death are different from those of other American physicians. I'm sure that the herrn inschpektors will soon be belaboring us with this IARC report, but I don't think there's any new evidence - or if there is, I wish the IARC or somebody would publish it. Bob Richmond well pickled samurai pathologist Knoxville TN From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Sep 21 01:39:36 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Sep 21 01:39:49 2007 Subject: [Histonet] NTMT woes Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222ED45@wahtntex2.waht.swest.nhs.uk> Think I said in an e-mail to the author of this thread that I think the magnesium chloride double decomposes with another salt to create the carbonate which is not soluble like the chloride. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; I want to work for a company that contributes to and is part of the community. I want something not just to invest in. I want something to believe in. --Anita Roddick This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Sep 21 01:40:48 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Sep 21 01:41:00 2007 Subject: [Histonet] NTMT woes Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222ED46@wahtntex2.waht.swest.nhs.uk> I stand corrected . Hydroxide, not carbonate. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; I want to work for a company that contributes to and is part of the community. I want something not just to invest in. I want something to believe in. --Anita Roddick This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From katia.catunda <@t> cipax.com.br Fri Sep 21 06:25:15 2007 From: katia.catunda <@t> cipax.com.br (=?iso-8859-1?Q?Ms._K=E1tia_Cristina_Catunda?=) Date: Fri Sep 21 06:25:13 2007 Subject: [Histonet] Potassium phosphate or Sodium phosphate? In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBF0222ED45@wahtntex2.waht.swest.nhs.uk> Message-ID: Hi there, Does anyone know advantages and disadvantages of using potassium phosphate buffer instead of sodium phosphate buffer on the rinsing of IHQ slides? Let me explain... we used to use sodium phosphate mono and dibasic with NaCL, now we are using potassium phosphate mono and sodium phosphate dibasic with NaCL.. Tks! Ms. K?tia Catunda Produ??o +55 12 3203-0612 (direto) +55 12 3203-0633 (PABX) www.cipax.com.br katia.catunda@cipax.com.br From katia.catunda <@t> cipax.com.br Fri Sep 21 06:30:03 2007 From: katia.catunda <@t> cipax.com.br (=?iso-8859-1?Q?Ms._K=E1tia_Cristina_Catunda?=) Date: Fri Sep 21 06:30:00 2007 Subject: [Histonet] Acetylcholinesterase protocol and fixation In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBF0222ED45@wahtntex2.waht.swest.nhs.uk> Message-ID: Another question... Looking for Acetylcholinesterase protocols I?ve found that probably Filipe and Lake?s is the most used, is this information correct? And... found using Karnovsky fixation or formaldehyde/calcium acetate.. which one is the best?? Tks again Ms. K?tia Catunda Produ??o +55 12 3203-0612 (direto) +55 12 3203-0633 (PABX) www.cipax.com.br katia.catunda@cipax.com.br From TJJ <@t> Stowers-Institute.org Fri Sep 21 07:10:28 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Fri Sep 21 07:12:04 2007 Subject: FW: [Histonet] NTMT woes References: <86ADE4EB583CE64799A9924684A0FBBF0222ED41@wahtntex2.waht.swest.nhs.uk> Message-ID: With permission from the email author - for your consideration. Thanks Kemlo! (makes sense of his email correction to the list of "hydroxide, not carbonate") -----Original Message----- From: Kemlo Rogerson [mailto:Kemlo.Rogerson@waht.swest.nhs.uk] Sent: Thu 9/20/2007 9:50 AM To: Johnson, Teri Subject: RE: [Histonet] NTMT woes Magnesium chloride is the name for the chemical compounds with the formulas MgCl2 and its various hydrates MgCl2(H2O)x. These salts are typical ionic halides, being highly soluble in water. The hydrated magnesium chloride can be extracted from brine or sea water. Anhydrous magnesium chloride is the principal precursor to magnesium metal, which is produced on a large scale. The most common magnesium carbonate forms are the anhydrous salt called magnesite (MgCO3) and the di, tri, and pentahydrates known as barringtonite (MgCO3*2H2O), nesquehonite (MgCO3*3H2O), and lansfordite (MgCO3*5H2O), respectively. Some basic forms such as artinite (MgCO3*Mg(OH)2*3H2O), hydromagnestite (4MgCO3*Mg(OH)2*4H2O), and dypingite (4MgCO3* Mg(OH)2*5H2O) also occur as minerals. Magnesite consists of white trigonal crystals. The anhydrous salt is practically insoluble in water, acetone, and ammonia. All forms of magnesium carbonate dissolve in acids. Magnesium carbonate crystallizes in the calcite structure wherein Mg2+ is surrounded by six oxygen atoms. The dihydrate has a triclinic structure, while the trihydrate has a monoclinic structure. Could it be there's a chemical reaction from chloride to carbonate with the subsequent falling out of solution? Isn't it called a double decomposition of a salt? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; I want to work for a company that contributes to and is part of the community. I want something not just to invest in. I want something to believe in. --Anita Roddick This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From oshel1pe <@t> cmich.edu Fri Sep 21 07:53:23 2007 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Fri Sep 21 07:53:40 2007 Subject: FW: [Histonet] NTMT woes In-Reply-To: References: <86ADE4EB583CE64799A9924684A0FBBF0222ED41@wahtntex2.waht.swest.nhs.uk> Message-ID: Terri, Thanks for forwarding this, and thanks to Kemlo for giving permission to forward. Interesing. Phil >With permission from the email author - for your consideration. > >Thanks Kemlo! > >(makes sense of his email correction to the list of "hydroxide, not >carbonate") > > >-----Original Message----- >From: Kemlo Rogerson [mailto:Kemlo.Rogerson@waht.swest.nhs.uk] >Sent: Thu 9/20/2007 9:50 AM >To: Johnson, Teri >Subject: RE: [Histonet] NTMT woes > >Magnesium chloride is the name for the chemical compounds with the >formulas MgCl2 and its various hydrates MgCl2(H2O)x. These salts are >typical ionic halides, being highly soluble in water. The hydrated >magnesium chloride can be extracted from brine or sea water. Anhydrous >magnesium chloride is the principal precursor to magnesium metal, which >is produced on a large scale. > >The most common magnesium carbonate forms are the anhydrous salt called >magnesite (MgCO3) and the di, tri, and pentahydrates known as >barringtonite (MgCO3*2H2O), nesquehonite (MgCO3*3H2O), and lansfordite >(MgCO3*5H2O), respectively. Some basic forms such as artinite >(MgCO3*Mg(OH)2*3H2O), hydromagnestite (4MgCO3*Mg(OH)2*4H2O), and >dypingite (4MgCO3* Mg(OH)2*5H2O) also occur as minerals. Magnesite >consists of white trigonal crystals. The anhydrous salt is practically >insoluble in water, acetone, and ammonia. All forms of magnesium >carbonate dissolve in acids. Magnesium carbonate crystallizes in the >calcite structure wherein Mg2+ is surrounded by six oxygen atoms. The >dihydrate has a triclinic structure, while the trihydrate has a >monoclinic structure. > >Could it be there's a chemical reaction from chloride to carbonate with >the subsequent falling out of solution? Isn't it called a double >decomposition of a salt? > >Kemlo Rogerson >Pathology Manager >DD 01934 647057 or extension 3311 >Mob 07749 754194; Pager 07659 597107; -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 From HornHV <@t> archildrens.org Fri Sep 21 10:03:58 2007 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Sep 21 11:32:25 2007 Subject: FW: [Histonet] Re: Formaldehyde - Carcinogen? Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB7361@EMAIL.archildrens.org> Histotechs have a high exposure to formalin as well and all of the deceased techs I know of died from cancer. Could be coincidental and lifestyle choices besides work related exposure would have to be considered. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Thursday, September 20, 2007 10:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Formaldehyde - Carcinogen? Beth A. Fye at HCA Richmond [Virginia?] Hospital Laboratories asks: >>In 2006, the IARC [International Agency for Research on Cancer] changed formaldehyde from a Group 2a - Probable Carcinogen to a Group I Carcinogenic to Humans. Has this changed... any procedures within [your] Histology Laboratory?<< See http://www.iarc.fr/ENG/Press_Releases/archives/pr153a.html I never heard of them, but I don't think the IARC is a regulatory agency - rather they're a subsidiary of the WHO (World Health Organization) based in France. Pathologists (at least this pathologist) have a very high lifetime exposure to formaldehyde. The AMA (American Medical Association) tracks the causes of death of American physicians very carefully. To date there has been no information published to suggest that pathologists' causes of death are different from those of other American physicians. I'm sure that the herrn inschpektors will soon be belaboring us with this IARC report, but I don't think there's any new evidence - or if there is, I wish the IARC or somebody would publish it. Bob Richmond well pickled samurai pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From Terry.Marshall <@t> rothgen.nhs.uk Fri Sep 21 10:45:36 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 21 11:51:59 2007 Subject: [Histonet] Re: Formaldehyde - Carcinogen? Message-ID: <407F05A128805F4C879A33DBA32E618E0189506D@TRFT-EX01.xRothGen.nhs.uk> "To date there has been no information published to suggest that pathologists' causes of death are different from those of other American physicians." Over here (UK) pathologists have the second highest suicide rate. This suggests that they haven't had time to get the long term effects of formalin:-) Terry - equally pickled. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: 21 September 2007 04:16 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Formaldehyde - Carcinogen? Beth A. Fye at HCA Richmond [Virginia?] Hospital Laboratories asks: >>In 2006, the IARC [International Agency for Research on Cancer] changed formaldehyde from a Group 2a - Probable Carcinogen to a Group I Carcinogenic to Humans. Has this changed... any procedures within [your] Histology Laboratory?<< See http://www.iarc.fr/ENG/Press_Releases/archives/pr153a.html I never heard of them, but I don't think the IARC is a regulatory agency - rather they're a subsidiary of the WHO (World Health Organization) based in France. Pathologists (at least this pathologist) have a very high lifetime exposure to formaldehyde. The AMA (American Medical Association) tracks the causes of death of American physicians very carefully. To date there has been no information published to suggest that pathologists' causes of death are different from those of other American physicians. I'm sure that the herrn inschpektors will soon be belaboring us with this IARC report, but I don't think there's any new evidence - or if there is, I wish the IARC or somebody would publish it. Bob Richmond well pickled samurai pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Fri Sep 21 11:24:50 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 21 11:56:06 2007 Subject: [Histonet] Acetylcholinesterase protocol and fixation In-Reply-To: References: <86ADE4EB583CE64799A9924684A0FBBF0222ED45@wahtntex2.waht.swest.nhs.uk> Message-ID: The AChE method in Filipe & Lake's book (Histochemistry in Pathology) is the one-step Karnovsky & Roots method, with inhibition of pseudocholinesterase by iso-OMPA and an intensification step using osmium tetroxide. It's intended for diagnosis of Hirschprung's disease - minimally fixed cryosections of rectal biopsies. The Karnovsky-Roots method is very reliable and in my experience (with brain and muscle) preferable to the Koelle-type methods. If you don't like using osmium tetroxide, there's an alternative intensification procedure using Nickel-DAB. See Tago G et al 1986 J. Histochem. Cytochem. 34: 1431-1438. The ChE inhibitor iso-OMPA is pretty toxic. Depending on the nature of your investigation, you may not need it. -- ------------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan[AT]uwo.ca http://publish.uwo.ca/~jkiernan/ http://instruct.uwo.ca/anatomy/530/index.htm _______________________________ -- Date: Friday, September 21, 2007 7:31 Subject: [Histonet] Acetylcholinesterase protocol and fixation To: histonet@lists.utsouthwestern.edu > Another question... > > Looking for Acetylcholinesterase protocols I?ve found that probably > Filipe and Lake?s is the most used, is this information correct? > > And... found using Karnovsky fixation or formaldehyde/calcium > acetate.. which one is the best?? > > Tks again > > > Ms. K?tia Catunda > Produ??o > +55 12 3203-0612 (direto) > +55 12 3203-0633 (PABX) > www.cipax.com.br > katia.catunda@cipax.com.br > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From PMonfils <@t> Lifespan.org Fri Sep 21 11:31:53 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Sep 21 11:56:12 2007 Subject: [Histonet] pan anti-mouse antibody? Message-ID: <4EBFF65383B74D49995298C4976D1D5E273CEB@LSRIEXCH1.lsmaster.lifespan.org> Does anyone use, or know of, an anti-mouse primary antibody that will stain all mouse cells, but not human cells? (Preferably in formalin-fixed tissue sections) From gu.lang <@t> gmx.at Fri Sep 21 12:14:42 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Sep 21 12:15:08 2007 Subject: AW: [Histonet] Re: Formaldehyde - Carcinogen? In-Reply-To: <407F05A128805F4C879A33DBA32E618E0189506D@TRFT-EX01.xRothGen.nhs.uk> Message-ID: <000001c7fc72$e6d8d5f0$6412a8c0@dielangs.at> Recently I read an article (in German) of an German office, that deals with dangerous stuff at work. This article says, that the save level of Formaldehyd should be at 0,1 ppm. They referred to studies with rats and monkeys, that showed increasing nose-mucosa-cancer at higher levels. Also the irritant property of formaldehyd, that causes an steady need of regeneration, has influence on its dangerousness. This irritation begins at levels above 0,1 ppm. The outcome of the article was (for me), that if you avoid the irritating exposure (with fumehoods), you are on the save side. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Marshall Terry Dr,Consultant Histopathologist Gesendet: Freitag, 21. September 2007 17:46 An: Robert Richmond; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] Re: Formaldehyde - Carcinogen? "To date there has been no information published to suggest that pathologists' causes of death are different from those of other American physicians." Over here (UK) pathologists have the second highest suicide rate. This suggests that they haven't had time to get the long term effects of formalin:-) Terry - equally pickled. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: 21 September 2007 04:16 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Formaldehyde - Carcinogen? Beth A. Fye at HCA Richmond [Virginia?] Hospital Laboratories asks: >>In 2006, the IARC [International Agency for Research on Cancer] changed formaldehyde from a Group 2a - Probable Carcinogen to a Group I Carcinogenic to Humans. Has this changed... any procedures within [your] Histology Laboratory?<< See http://www.iarc.fr/ENG/Press_Releases/archives/pr153a.html I never heard of them, but I don't think the IARC is a regulatory agency - rather they're a subsidiary of the WHO (World Health Organization) based in France. Pathologists (at least this pathologist) have a very high lifetime exposure to formaldehyde. The AMA (American Medical Association) tracks the causes of death of American physicians very carefully. To date there has been no information published to suggest that pathologists' causes of death are different from those of other American physicians. I'm sure that the herrn inschpektors will soon be belaboring us with this IARC report, but I don't think there's any new evidence - or if there is, I wish the IARC or somebody would publish it. Bob Richmond well pickled samurai pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Fri Sep 21 12:22:01 2007 From: tkngflght <@t> yahoo.com (Cheryl R. Kerry) Date: Fri Sep 21 12:22:01 2007 Subject: OT on NBF--RE: [Histonet] Re: Formaldehyde - Carcinogen? In-Reply-To: <000001c7fc72$e6d8d5f0$6412a8c0@dielangs.at> Message-ID: <002401c7fc73$ecdb6bb0$6701a8c0@CHERYLSLAPTOP> I'm old-school and have had my share of exposure. I think all the formaldehyde has help me maintain my youthful countenance! I don't have wrinkles at 43 and it's all from NBF fumes!! I won't need to be embalmed--just tuck me in and drop the lid. (It's Friday folks--just playing around :) ) Cheryl Cheryl R. Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab, one GREAT tech at a time! 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone -----Original Message----- From jnocito <@t> satx.rr.com Fri Sep 21 12:27:06 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Sep 21 12:27:22 2007 Subject: OT on NBF--RE: [Histonet] Re: Formaldehyde - Carcinogen? References: <002401c7fc73$ecdb6bb0$6701a8c0@CHERYLSLAPTOP> Message-ID: <007601c7fc74$a249cd20$0202a8c0@yourxhtr8hvc4p> says, says, says u, I, I, I, don, don don't know, know wh, wh, where, thi, thi, this stu, stu stutter came from JTT ----- Original Message ----- From: "Cheryl R. Kerry" To: Sent: Friday, September 21, 2007 12:22 PM Subject: OT on NBF--RE: [Histonet] Re: Formaldehyde - Carcinogen? > > I'm old-school and have had my share of exposure. I think all the > formaldehyde has help me maintain my youthful countenance! I don't have > wrinkles at 43 and it's all from NBF fumes!! I won't need to be > embalmed--just tuck me in and drop the lid. > > (It's Friday folks--just playing around :) ) > > Cheryl > > Cheryl R. Kerry, HT(ASCP) > Full Staff Inc. > Staffing the AP Lab, one GREAT tech at a time! > 281.852.9457 office > 281.883.7704 cell > 800.756.3309 fax and alternate phone > -----Original Message----- > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Sep 21 14:49:56 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Sep 21 14:50:08 2007 Subject: [Histonet] Potassium phosphate or Sodium phosphate? In-Reply-To: Message-ID: <840774.10619.qm@web61217.mail.yahoo.com> The buffer action resides in the phosphate molecule acting as anion (negatively charged). Either sodium or potassium act as cations (+ charge, of equal charge) and are similar in reaction, so there are no theoretical advantages of one (K) over the other (Na). Ren? J. "Ms. K?tia Cristina Catunda" wrote: Hi there, Does anyone know advantages and disadvantages of using potassium phosphate buffer instead of sodium phosphate buffer on the rinsing of IHQ slides? Let me explain... we used to use sodium phosphate mono and dibasic with NaCL, now we are using potassium phosphate mono and sodium phosphate dibasic with NaCL.. Tks! Ms. K?tia Catunda Produ??o +55 12 3203-0612 (direto) +55 12 3203-0633 (PABX) www.cipax.com.br katia.catunda@cipax.com.br _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Fussy? Opinionated? Impossible to please? Perfect. Join Yahoo!'s user panel and lay it on us. From tim.morken <@t> thermofisher.com Fri Sep 21 15:42:29 2007 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Fri Sep 21 15:42:59 2007 Subject: [Histonet] Potassium phosphate or Sodium phosphate? In-Reply-To: <840774.10619.qm@web61217.mail.yahoo.com> References: <840774.10619.qm@web61217.mail.yahoo.com> Message-ID: <6BFF6D137DF6BC43B33891BA96E83B19C0DBBD@PGHCR-EXMB-VS-1.na.fshrnet.com> Rene is right that for immunohistochemistry on fixed tissue there is no functional differences between sodium and potassium phosphate salts for buffers. The critical factor in a wash buffer for IHC is the ion concentration ("strength" if you wish), not the type of salt. This all got started with buffers designed for live cells and tissues. The original Dulbecco's phosphate buffer was designed to mimic the type and concentration of the various salts in normal human blood and body fluids in order to make living cells happy. The adaption of that formulation to fixed cell/tissue procedures has caused a lot of confusion over the years. Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, September 21, 2007 12:50 PM To: Ms. K?tia Cristina Catunda; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Potassium phosphate or Sodium phosphate? The buffer action resides in the phosphate molecule acting as anion (negatively charged). Either sodium or potassium act as cations (+ charge, of equal charge) and are similar in reaction, so there are no theoretical advantages of one (K) over the other (Na). Ren? J. "Ms. K?tia Cristina Catunda" wrote: Hi there, Does anyone know advantages and disadvantages of using potassium phosphate buffer instead of sodium phosphate buffer on the rinsing of IHQ slides? Let me explain... we used to use sodium phosphate mono and dibasic with NaCL, now we are using potassium phosphate mono and sodium phosphate dibasic with NaCL.. Tks! Ms. K?tia Catunda Produ??o +55 12 3203-0612 (direto) +55 12 3203-0633 (PABX) www.cipax.com.br katia.catunda@cipax.com.br _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Fussy? Opinionated? Impossible to please? Perfect. Join Yahoo!'s user panel and lay it on us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From agustinvictor <@t> gmail.com Fri Sep 21 17:42:00 2007 From: agustinvictor <@t> gmail.com (Agustin Chertcoff) Date: Fri Sep 21 17:43:13 2007 Subject: [Histonet] SAHNET Message-ID: <012701c7fca0$a1b418d0$590215ac@Pentium4> ----- Original Message ----- From: Agustin Chertcoff To: histonet@lists.utsouthwestern.edu Sent: Sunday, September 09, 2007 12:35 AM Subject: SAHNET Hi Histonetters! The SAHNET is a listserver (in Spanish Language) for the Histology profession that is managed as a service to the field of Histology by Ht Agustin Victor Chertcoff & Ht Norma Pozzo. SAHNET current has more than 245 members in the world specially Argentine,Peru,Venezuela,Mexico,Costa Rica,Uruguay,Chile,Colombia,Spain. go to http://www.elistas.net/grupo/sahnet visit to www.ht.org.ar Argentine Society of Histotechnology SAH Thanks to all! Agustin From szhang101 <@t> hotmail.com Fri Sep 21 20:02:35 2007 From: szhang101 <@t> hotmail.com (Shengwen Zhang) Date: Fri Sep 21 19:03:06 2007 Subject: [Histonet] Thin slice embedding Message-ID: Hi All, I'm trying to further cut my organotypically cultured brain slices (300 micron before culture, ~100 micron after culture). I've tried OTC compound and sucrose solutions for embedding, both didn't work well (the slices wrinkle at the tissue-medium boundary). Now I want to try paraffin. I want to keep 3-4 slices from the same brain flat and on the same plane, so I can process them together (otherwise, I would have too much work to do). I created a flat area with paraffin, after it solidified, I laid the slices, and pour another layer of paraffin. But the two layers don't stick together well enough to stand the forces during cutting. How do you embed thin slices with paraffin? Thank you! Shengwen From clfields12 <@t> alltel.net Fri Sep 21 20:25:15 2007 From: clfields12 <@t> alltel.net (clfields12@alltel.net) Date: Fri Sep 21 20:25:30 2007 Subject: [Histonet] Histonet...how to subscribe? Message-ID: <20070922012515.IYKM19750.ispmxmta05-srv.windstream.net@webmail-relay.alltel.net> Hi, Could someone please tell me why it is so difficult to get on the Histonet? When I changed jobs they thought I was impersonating myself when I tried to subscrib and now I tried to get on at work as well as home and it has to go to the administrator for consideration again. Last time I had to get a friend to contact "the powers that be" to vouch for me. What in the world is going on...it used to be easy! Do we have a lot of people impersonating histo techs? Sorry to rant but this is nuts! THX Carole Fields, HT(ASCP) From tjasper <@t> copc.net Fri Sep 21 20:35:47 2007 From: tjasper <@t> copc.net (Thomas Jasper) Date: Fri Sep 21 20:36:00 2007 Subject: [Histonet] Histology Position Message-ID: <90354A475B420441B2A0396E5008D4965E1FA9@copc-sbs.COPC.local> Hello Everyone, I am re-posting our open position here in beautiful Bend, Oregon... Central Oregon Regional Pathology Services, Bend Oregon is seeking an HT or HTL, ASCP registered or registry eligible preferred. Full-time position with rotating weekend coverage. Responsibilities include embedding, microtomy, routine staining, automated immunohistochemistry and special staining. Excellent benefits, including health insurance and retirement plan and competitive salary. Potential sign-on bonus as well. Bend is located in central Oregon at the base of the Cascade Mountains. Hiking, backpacking, rock climbing, fishing and skiing opportunities abound. Bend is uniquely situated with the Cascade Mountains to the west and the high desert to the east. Although in the Pacific Northwest, Bend enjoys warm, clear days and cool evenings for much of the year. Winters are mild, the environment is clean and wilderness is accessible. In 15-20 minutes phenomenal skiing is available in the Cascades. In about 30 minutes you can reach the scenic beauty of canyons and rock formation in the high desert. Crater Lake national park is approximately 100 miles to the south and the Pacific coast of Oregon can be reached in about 3.5 hours (both are breathtaking). The lush Willamette Valley lies between the Cascades and the coast and is stunningly beautiful as well. Motorcycle and bicycle enthusiasts also enjoy long riding seasons, with many mountain roads for motorcycles to explore and abundant bicycle trails for serious or casual bicyclers. There are 3 local microbreweries in town which produce top notch beer (high praise from a Wisconsinite). Many wonderful restaurants of varied cuisine are located all over town. The University of Oregon (Eugene) and Oregon State University (Corvallis) are in close proximity as well. And if you really need that metropolitan fix... Portland is only a few hours away. We are looking to build and solidify our work team, if you are interested please do not hesitate to contact us. Central Oregon Regional Pathology Services, 1348 NE Cushing Dr., Bend, OR 97701 Attn: Pam Sylvester or fax resume to: (541)693-2648 Thanks, if you have any questions you may contact me as well. Thomas Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 tjasper@copc.net 541/693-2877 ext. 4050 From jkiernan <@t> uwo.ca Fri Sep 21 21:10:27 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 21 21:10:44 2007 Subject: [Histonet] Potassium phosphate or Sodium phosphate? Message-ID: For rinsing in immunohistochemistry, it will not make any difference. In a fixative such as buffered formaldehyde or glutaraldehyde, it is usual to use sodium phosphates because sodium is the predominant cation in animal extracellular fluids. (Potassium predominates in cytoplasm.) Does it make any difference when the fixative is for light microscopy? Has anyone done a comparison? John Kiernan Anatomy, UWO London, Canada --- ----- Original Message ----- From: Rene J Buesa Date: Friday, September 21, 2007 15:51 Subject: Re: [Histonet] Potassium phosphate or Sodium phosphate? To: "Ms. K?tia Cristina Catunda" , histonet@lists.utsouthwestern.edu > The buffer action resides in the phosphate molecule acting as > anion (negatively charged). Either sodium or potassium act as > cations (+ charge, of equal charge) and are similar in reaction, > so there are no theoretical advantages of one (K) over the other (Na). > Ren? J. > > "Ms. K?tia Cristina Catunda" wrote: > Hi there, > > Does anyone know advantages and disadvantages of using potassium > phosphate buffer instead of sodium phosphate buffer on the > rinsing of IHQ > slides? > > Let me explain... we used to use sodium phosphate mono and dibasic > with NaCL, now we are using potassium phosphate mono and sodium > phosphatedibasic with NaCL.. > > Tks! > > Ms. K?tia Catunda > Produ??o > +55 12 3203-0612 (direto) > +55 12 3203-0633 (PABX) > www.cipax.com.br > katia.catunda@cipax.com.br > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Fussy? Opinionated? Impossible to please? Perfect. Join > Yahoo!'s user panel and lay it on us. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Sat Sep 22 07:47:34 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Sep 22 07:47:47 2007 Subject: [Histonet] Thin slice embedding In-Reply-To: Message-ID: <773202.29867.qm@web61217.mail.yahoo.com> If I understood you well you are trying to "hold" slices of tissue flat to further section them. If that is the case preparing a layer of paraffin, waiting it to solidify and adding later on the tissue and another layer of paraffin (like a sandwich) did not work, and will not work. Try the following: get a metal flat surface the size of you tissue slices. Pour melted paraffin in a mold larger than the piece of tissue. Put the piece of tissue flat OVER the melted paraffin, and the metal flat surface HEATED above the melting point of the paraffin, PUSH the piece of tissue up to the depth you want. Gently retrieve (PULL) the piece of metal leaving the piece of tissue at the depth you selected. Wait until this "block" solidifies and try to section. Since the piece of tissue has not been infiltrated, just embedded, the paraffin will not hold to the tissue, but keep it in position to section. Ren? J. Shengwen Zhang wrote: Hi All, I'm trying to further cut my organotypically cultured brain slices (300 micron before culture, ~100 micron after culture). I've tried OTC compound and sucrose solutions for embedding, both didn't work well (the slices wrinkle at the tissue-medium boundary). Now I want to try paraffin. I want to keep 3-4 slices from the same brain flat and on the same plane, so I can process them together (otherwise, I would have too much work to do). I created a flat area with paraffin, after it solidified, I laid the slices, and pour another layer of paraffin. But the two layers don't stick together well enough to stand the forces during cutting. How do you embed thin slices with paraffin? Thank you! Shengwen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Pinpoint customers who are looking for what you sell. From moran.elish <@t> gmail.com Sun Sep 23 09:13:51 2007 From: moran.elish <@t> gmail.com (Moran Elishmereni) Date: Sun Sep 23 09:14:12 2007 Subject: [Histonet] IHC together with toluidine blue staining for mast cells Message-ID: <4a722ef70709230713h144b7563o3c96dfde21b1ee95@mail.gmail.com> Hi Histonetters, I was wondering if anyone knows how to stain paraffin-embedded tissues for a certain Ab (standard IHC), and use toluidine blue to stain mast cells on the same section. Is it at all possible? I emphasize that I would like to use toluidine blue not for counterstaining, but to detect mast cells, and to still be able to see my IHC staining. Glad to recieve any piece of advice. Thanks, Moran -- Moran Elishmereni Department of Pharmacology and Experimental Therapeutics School of Pharmacy, Faculty of Medicine The Hebrew University of Jerusalem POB 12065 Jerusalem 91120, ISRAEL Tel: 972-2-675-8746 Fax: 972-2-675-8144 Email: moran.elish@gmail.com From rjbuesa <@t> yahoo.com Sun Sep 23 09:33:53 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Sep 23 09:34:05 2007 Subject: [Histonet] IHC together with toluidine blue staining for mast cells In-Reply-To: <4a722ef70709230713h144b7563o3c96dfde21b1ee95@mail.gmail.com> Message-ID: <35113.54839.qm@web61223.mail.yahoo.com> IF your antibody is FOR mastcells, you will block the toluidine blue staining sites with the DAB substrate, but IF you are using an Ab for a different target, then you can stain the mast cells with toluidine blue, but that will be "like" a counterstain. Ren? J. Moran Elishmereni wrote: Hi Histonetters, I was wondering if anyone knows how to stain paraffin-embedded tissues for a certain Ab (standard IHC), and use toluidine blue to stain mast cells on the same section. Is it at all possible? I emphasize that I would like to use toluidine blue not for counterstaining, but to detect mast cells, and to still be able to see my IHC staining. Glad to recieve any piece of advice. Thanks, Moran -- Moran Elishmereni Department of Pharmacology and Experimental Therapeutics School of Pharmacy, Faculty of Medicine The Hebrew University of Jerusalem POB 12065 Jerusalem 91120, ISRAEL Tel: 972-2-675-8746 Fax: 972-2-675-8144 Email: moran.elish@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better Globetrotter. Get better travel answers from someone who knows. Yahoo! Answers - Check it out. From Kemlo.Rogerson <@t> waht.swest.nhs.uk Mon Sep 24 02:56:55 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Sep 24 02:57:07 2007 Subject: [Histonet] Re: Formaldehyde - Carcinogen? Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222ED5F@wahtntex2.waht.swest.nhs.uk> "To date there has been no information published to suggest that pathologists' causes of death are different from those of other American physicians." Over here (UK) pathologists have the second highest suicide rate. This suggests that they haven't had time to get the long term effects of formalin:-) Terry - equally pickled. Terry was it the formalin that caused you to be so 'pickled' or another type of fixative? Who has the highest suicide rate? I don't actually know of any Pathologists that has committed suicide, only a few whose suicide was professional. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Loneliness Is An island In The middle Of A sea of people --R.R. (age 11) This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Terry.Marshall <@t> rothgen.nhs.uk Mon Sep 24 06:10:40 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Mon Sep 24 06:10:53 2007 Subject: [Histonet] Re: Formaldehyde - Carcinogen? Message-ID: <407F05A128805F4C879A33DBA32E618E01895079@TRFT-EX01.xRothGen.nhs.uk> Formalin. Psychiatrists. Terry -----Original Message----- From: Kemlo Rogerson [mailto:Kemlo.Rogerson@waht.swest.nhs.uk] Sent: 24 September 2007 08:57 To: Marshall Terry Dr, Consultant Histopathologist; Robert Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Formaldehyde - Carcinogen? "To date there has been no information published to suggest that pathologists' causes of death are different from those of other American physicians." Over here (UK) pathologists have the second highest suicide rate. This suggests that they haven't had time to get the long term effects of formalin:-) Terry - equally pickled. Terry was it the formalin that caused you to be so 'pickled' or another type of fixative? Who has the highest suicide rate? I don't actually know of any Pathologists that has committed suicide, only a few whose suicide was professional. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Loneliness Is An island In The middle Of A sea of people --R.R. (age 11) This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From cmiller <@t> physlab.com Mon Sep 24 09:11:20 2007 From: cmiller <@t> physlab.com (Cheri Miller) Date: Mon Sep 24 09:11:42 2007 Subject: [Histonet] Re: Formaldehyde - Carcinogen? In-Reply-To: <407F05A128805F4C879A33DBA32E618E01895079@TRFT-EX01.xRothGen.nhs.uk> References: <407F05A128805F4C879A33DBA32E618E01895079@TRFT-EX01.xRothGen.nhs.uk> Message-ID: <000001c7feb4$c9b6b070$3402a8c0@plab.local> That is due to the fact that most pathologists don't due grossing beyond their Pathology Internship. Every path that I know has a rare exposure to formalin. It's the PA's, dieners and techs that are exposed. Just my 2 cents no flaming please I am sensitive. :) Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Monday, September 24, 2007 6:11 AM To: Kemlo Rogerson; Robert Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Formaldehyde - Carcinogen? Formalin. Psychiatrists. Terry -----Original Message----- From: Kemlo Rogerson [mailto:Kemlo.Rogerson@waht.swest.nhs.uk] Sent: 24 September 2007 08:57 To: Marshall Terry Dr, Consultant Histopathologist; Robert Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Formaldehyde - Carcinogen? "To date there has been no information published to suggest that pathologists' causes of death are different from those of other American physicians." Over here (UK) pathologists have the second highest suicide rate. This suggests that they haven't had time to get the long term effects of formalin:-) Terry - equally pickled. Terry was it the formalin that caused you to be so 'pickled' or another type of fixative? Who has the highest suicide rate? I don't actually know of any Pathologists that has committed suicide, only a few whose suicide was professional. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Loneliness Is An island In The middle Of A sea of people --R.R. (age 11) This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From TJJ <@t> Stowers-Institute.org Mon Sep 24 09:34:38 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Mon Sep 24 09:35:04 2007 Subject: [Histonet] Re: IHC together with toluidine blue staining for mast cells In-Reply-To: Message-ID: Moran, I find that in IHC procedures mast cell granules take up the secondary label. If you are doing this to try to see if your IHC is labeling mast cells, I agree with Rene's assessment - I doubt you will get histochemical staining over the chromogen, but it might be worth a try. Do your IHC procedure first with DAB, then you will stain with Tol. Blue, air dry, and mount with permanent mounting medium. The Tol blue will also stain your nuclei, just as if you were doing a regular nuclear counterstain. If this stain will obscure nuclear IHC signal, consider doing the Tolduidine blue stain first, coverslip, photograph, decoverslip and hydrate back to water, and then perform your IHC procedure, coverslip, and photograph. No need to remove the Tolduidine blue before doing your IHC procedures, as it should wash out in exposure to alcohols and water prior to and during the IHC procedures. Hope this helps! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From cmiller <@t> physlab.com Mon Sep 24 09:34:54 2007 From: cmiller <@t> physlab.com (Cheri Miller) Date: Mon Sep 24 09:35:12 2007 Subject: [Histonet] Re: Formaldehyde - Carcinogen? In-Reply-To: <000001c7feb4$c9b6b070$3402a8c0@plab.local> References: <407F05A128805F4C879A33DBA32E618E01895079@TRFT-EX01.xRothGen.nhs.uk> <000001c7feb4$c9b6b070$3402a8c0@plab.local> Message-ID: <000801c7feb8$14ef8eb0$3402a8c0@plab.local> DUE?? Sorry make that Do Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Monday, September 24, 2007 9:11 AM To: 'Marshall Terry Dr,Consultant Histopathologist'; 'Kemlo Rogerson'; 'Robert Richmond'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Formaldehyde - Carcinogen? That is due to the fact that most pathologists don't due grossing beyond their Pathology Internship. Every path that I know has a rare exposure to formalin. It's the PA's, dieners and techs that are exposed. Just my 2 cents no flaming please I am sensitive. :) Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Monday, September 24, 2007 6:11 AM To: Kemlo Rogerson; Robert Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Formaldehyde - Carcinogen? Formalin. Psychiatrists. Terry -----Original Message----- From: Kemlo Rogerson [mailto:Kemlo.Rogerson@waht.swest.nhs.uk] Sent: 24 September 2007 08:57 To: Marshall Terry Dr, Consultant Histopathologist; Robert Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Formaldehyde - Carcinogen? "To date there has been no information published to suggest that pathologists' causes of death are different from those of other American physicians." Over here (UK) pathologists have the second highest suicide rate. This suggests that they haven't had time to get the long term effects of formalin:-) Terry - equally pickled. Terry was it the formalin that caused you to be so 'pickled' or another type of fixative? Who has the highest suicide rate? I don't actually know of any Pathologists that has committed suicide, only a few whose suicide was professional. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Loneliness Is An island In The middle Of A sea of people --R.R. (age 11) This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From Terry.Marshall <@t> rothgen.nhs.uk Mon Sep 24 09:46:57 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Mon Sep 24 09:47:50 2007 Subject: [Histonet] Re: Formaldehyde - Carcinogen? Message-ID: <407F05A128805F4C879A33DBA32E618E0189507A@TRFT-EX01.xRothGen.nhs.uk> That's a great post to read first after getting back from an 1 hr 45 mins cut-up! :-) Terry -----Original Message----- From: Cheri Miller [mailto:cmiller@physlab.com] Sent: 24 September 2007 15:11 To: Marshall Terry Dr, Consultant Histopathologist; 'Kemlo Rogerson'; 'Robert Richmond'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Formaldehyde - Carcinogen? That is due to the fact that most pathologists don't due grossing beyond their Pathology Internship. Every path that I know has a rare exposure to formalin. It's the PA's, dieners and techs that are exposed. Just my 2 cents no flaming please I am sensitive. :) Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Monday, September 24, 2007 6:11 AM To: Kemlo Rogerson; Robert Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Formaldehyde - Carcinogen? Formalin. Psychiatrists. Terry -----Original Message----- From: Kemlo Rogerson [mailto:Kemlo.Rogerson@waht.swest.nhs.uk] Sent: 24 September 2007 08:57 To: Marshall Terry Dr, Consultant Histopathologist; Robert Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Formaldehyde - Carcinogen? "To date there has been no information published to suggest that pathologists' causes of death are different from those of other American physicians." Over here (UK) pathologists have the second highest suicide rate. This suggests that they haven't had time to get the long term effects of formalin:-) Terry - equally pickled. Terry was it the formalin that caused you to be so 'pickled' or another type of fixative? Who has the highest suicide rate? I don't actually know of any Pathologists that has committed suicide, only a few whose suicide was professional. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Loneliness Is An island In The middle Of A sea of people --R.R. (age 11) This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From Terry.Marshall <@t> rothgen.nhs.uk Mon Sep 24 10:21:14 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Mon Sep 24 10:39:15 2007 Subject: [Histonet] Re: Formaldehyde - Carcinogen? Message-ID: <407F05A128805F4C879A33DBA32E618E0189507B@TRFT-EX01.xRothGen.nhs.uk> Rene has privately advised me that cut-up = grossing on the other side of the pond. Whatever, both are as terms go, pretty gross. Does anybody actually enjoy it? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: 24 September 2007 15:47 To: Cheri Miller; Kemlo Rogerson; Robert Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Formaldehyde - Carcinogen? That's a great post to read first after getting back from an 1 hr 45 mins cut-up! :-) Terry -----Original Message----- From: Cheri Miller [mailto:cmiller@physlab.com] Sent: 24 September 2007 15:11 To: Marshall Terry Dr, Consultant Histopathologist; 'Kemlo Rogerson'; 'Robert Richmond'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Formaldehyde - Carcinogen? That is due to the fact that most pathologists don't due grossing beyond their Pathology Internship. Every path that I know has a rare exposure to formalin. It's the PA's, dieners and techs that are exposed. Just my 2 cents no flaming please I am sensitive. :) Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Monday, September 24, 2007 6:11 AM To: Kemlo Rogerson; Robert Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Formaldehyde - Carcinogen? Formalin. Psychiatrists. Terry -----Original Message----- From: Kemlo Rogerson [mailto:Kemlo.Rogerson@waht.swest.nhs.uk] Sent: 24 September 2007 08:57 To: Marshall Terry Dr, Consultant Histopathologist; Robert Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Formaldehyde - Carcinogen? "To date there has been no information published to suggest that pathologists' causes of death are different from those of other American physicians." Over here (UK) pathologists have the second highest suicide rate. This suggests that they haven't had time to get the long term effects of formalin:-) Terry - equally pickled. Terry was it the formalin that caused you to be so 'pickled' or another type of fixative? Who has the highest suicide rate? I don't actually know of any Pathologists that has committed suicide, only a few whose suicide was professional. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Loneliness Is An island In The middle Of A sea of people --R.R. (age 11) This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Mon Sep 24 10:23:55 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Mon Sep 24 10:39:19 2007 Subject: [Histonet] OT - turnaround time Message-ID: <407F05A128805F4C879A33DBA32E618E0189507C@TRFT-EX01.xRothGen.nhs.uk> No - not that turnaround time ... The last message I posted arrived before my mouse had even left the "send" box. I have never seen anything like it. Terry From PMEarle <@t> CapeCodHealth.org Mon Sep 24 10:34:43 2007 From: PMEarle <@t> CapeCodHealth.org (Earle, Paul M.) Date: Mon Sep 24 10:39:22 2007 Subject: [Histonet] Opening for Histotech Message-ID: <29688FCA0750AD4FB55B18D961EB91CC101710@cchex2.cchdomain1.capecodhealth.org> We still have a position for a full time histotech position at Falmouth Hospital, located on beautiful Cape Cod, MA. Ours is a small path laboratory, 10K surgicals per year, two other Histotechs, day shift, full benefits, and competitive salary. Cape Cod is a resort community with wonderful saltwater beaches, over 200 freshwater ponds and lakes, and a mild climate. Whether you are looking for a great place to raise a family or for boating and fishing, this is a location to be considered. Contact pmearle <@t> capecodhealth.org Paul M. Earle M.S., P.A. (ASCP) Anatomic Pathology Supervisor Pathology Department Falmouth Hospital 100 Ter Heun Drive Falmouth, MA 02540 508-548-5300 x73488 <> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org From Kemlo.Rogerson <@t> waht.swest.nhs.uk Mon Sep 24 09:53:06 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Sep 24 10:39:46 2007 Subject: [Histonet] Re: Formaldehyde - Carcinogen? Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222ED74@wahtntex2.waht.swest.nhs.uk> Leave it to your BMSs then; you'll have more time to report. Anyone can cut up the meat it takes talent to cook it (g). Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Loneliness Is An island In The middle Of A sea of people --R.R. (age 11) This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Kemlo.Rogerson <@t> waht.swest.nhs.uk Mon Sep 24 09:44:56 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Sep 24 10:39:49 2007 Subject: [Histonet] Re: Formaldehyde - Carcinogen? Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222ED72@wahtntex2.waht.swest.nhs.uk> Interestingly 'who does the grossing' varies grossly in the UK. When I was a BMS in Histology I've 'grossed' gall bladders, uteri, appendix, obviously biopsies and many, many skin tumours. I guess when I worked in London as a histology/ cytology manager I was 'grossing' at least 80% of the work. In other Labs I've been in BMSs only 'put in' and don't draw the knife through anything. I wonder if that will change with the 'new' IBMS specialist exams or if the Pathologists will be reluctant to 'let go'? What do you think Terry, you were a Pathologists 'before your time' do you think BMSs/ Histotechs ought to gross and what ought they to dissect? My golden rule was that if I felt the Certainly saves you Pathologists exposure to formalin just BSE, prions and CJD to avoid!! Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Loneliness Is An island In The middle Of A sea of people --R.R. (age 11) This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From valeria.berno <@t> embl.it Mon Sep 24 11:23:34 2007 From: valeria.berno <@t> embl.it (Valeria Berno) Date: Mon Sep 24 11:24:06 2007 Subject: [Histonet] whole mount Message-ID: Hi, I was just wondering if it is common to use Sudan Black or other autofluorescence quenching method on whole mounting.It is the first time I am approaching whole mount immuno!!. Is there any special suggestion as my antigene has a very low expression?? thanks in advance Valeria Berno Valeria, PhD EMBL Monterotondo Outstation via Ramarini 32 00015 Monterotondo Scalo (RM) Italy Tel: +39 06 90091 287 Fax: +39 06 90091 272 HYPERLINK "mailto:valeria.berno@embl.it"valeria.berno@embl.it No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.5.488 / Virus Database: 269.13.30/1025 - Release Date: 23/09/2007 13.53 From jessgrocki <@t> yahoo.com Mon Sep 24 11:33:54 2007 From: jessgrocki <@t> yahoo.com (Jessica Piche) Date: Mon Sep 24 11:34:07 2007 Subject: [Histonet] D-40 Message-ID: <463687.24926.qm@web82008.mail.mud.yahoo.com> Hello,] I was wondering/hoping that someone out there is using D2-40 from Covance, and that they would be willing to share their protocol with me? If anyone out there is using it, I would really appreciate your help. THanks, Jessica Piche-Grocki, HT(ASCP) From Charles.Embrey <@t> carle.com Mon Sep 24 12:27:05 2007 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Mon Sep 24 12:30:47 2007 Subject: [Histonet] Histology Manager needed Message-ID: <44780C571F28624DBB446DE55C4D733A1FE545@EXCHANGEBE1.carle.com> I just wanted to pass this on to the list....... Carle Clinic Association is seeking a lab professional for a full time Histology Manager position. This individual will be responsible for the management of Histology. Will direct and monitor all personnel, technical and quality assurance activities, and maintain lab adherence to all regulatory (CLIA, CAP, OSHA) standards, as well as monitor and prepare budget, select personnel and develop and maintain monthly productivity benchmarking. The laboratory is a state-of-the-art facility offering a full range of clinical laboratory services. We offer a competitive salary in addition to a comprehensive employee benefit package featuring health insurance, paid leave program, life insurance, long term disability and a profit sharing plan and a $5,000 sign-on bonus for this position. Carle Clinic Association is a multi-specialty medical group practice of nearly 300 physicians affiliated with Carle Foundation Hospital, a 300-bed tertiary care facility whose services include a Level I Trauma Center, a growing Heart Center performing open heart surgery and the nationally renowned Cancer Center. As a regional referral center for a 42-county area in Central Illinois and Western Indiana, we serve a very diverse patient population. Carle Clinic is located in Urbana, home of the Big Ten University of Illinois and located within 2 ? hours of Chicago, St. Louis and Indianapolis. We require ASCP certification for HT or HTL with a minimum of five years experience in Histology plus strong leadership skills. Contact: Carle Clinic Association, Human Resource Services, 602 W. University Avenue, Urbana, IL 61801; 217.383.3238 or on line at www.carle.com/cliniccareers. From Shirley_PHUA <@t> hsa.gov.sg Mon Sep 24 13:02:35 2007 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Mon Sep 24 13:33:15 2007 Subject: [Histonet] Shirley Phua is away on 24 Sep 2007 (Monday) afternoon. Message-ID: I will be out of the office from 24-09-2007 to 25-09-2007. I'll be away on 24 Sep 2007 (Monday) afternoon. I'll be back on 24 Sep 2007 (Tuesday). Pathologists: I will process your requests when I return. If urgent, please forward your email to Henry_Kyaw@hsa.gov.sg From jkiernan <@t> uwo.ca Mon Sep 24 13:12:57 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Mon Sep 24 13:33:20 2007 Subject: [Histonet] Re: IHC together with toluidine blue staining for mast cells In-Reply-To: References: Message-ID: May I suggest first staining the heparin in the mast cells with alcian blue, pH 1.0, which gives an indestructible coloration. (Cartilage matrix and some kinds of mucus are also stained; shouldn't cause confusion unless you're working with gut mucosa. Nuclei are not stained.) Then do the immunohistochemistry. If there is a problem with nonspecific binding of DAB to mast cells, followed by its oxidation to a brown product, use a different labelling enzyme/chromogen. Remember that in many species mast cell granules are largely removed by commonly used aqueous fixatives, including neutral formaldehyde, though this is OK for rats and mice. See "The Mast Cells" by Hans Selye (Washington: Butterworths, 1965, pp 25-26) for summaries of 13 papers and citations of 5 review articles on fixation of mast cells. -- ------------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan[AT]uwo.ca http://publish.uwo.ca/~jkiernan/ http://instruct.uwo.ca/anatomy/530/index.htm _______________________________ ----- Original Message ----- From: "Johnson, Teri" Date: Monday, September 24, 2007 10:43 Subject: [Histonet] Re: IHC together with toluidine blue staining for mast cells To: histonet@lists.utsouthwestern.edu > Moran, I find that in IHC procedures mast cell granules take up the > secondary label. If you are doing this to try to see if your IHC is > labeling mast cells, I agree with Rene's assessment - I doubt > you will > get histochemical staining over the chromogen, but it might be > worth a > try. > > Do your IHC procedure first with DAB, then you will stain with Tol. > Blue, air dry, and mount with permanent mounting medium. The Tol blue > will also stain your nuclei, just as if you were doing a regular > nuclearcounterstain. > > If this stain will obscure nuclear IHC signal, consider doing the > Tolduidine blue stain first, coverslip, photograph, decoverslip and > hydrate back to water, and then perform your IHC procedure, coverslip, > and photograph. No need to remove the Tolduidine blue > before doing your > IHC procedures, as it should wash out in exposure to alcohols > and water > prior to and during the IHC procedures. > > Hope this helps! > > Teri Johnson, HT(ASCP)QIHC > Managing Director Histology Facility > Stowers Institute for Medical Research > 1000 E. 50th St. > Kansas City, MO 64110 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ccross6032 <@t> aol.com Mon Sep 24 14:07:40 2007 From: ccross6032 <@t> aol.com (Cheryl Cross) Date: Mon Sep 24 14:08:06 2007 Subject: [Histonet] biblio help? laboratory methods in histotechnology.... In-Reply-To: References: Message-ID: <157CA2E8-F881-47CB-9F1A-A2F07BA3D6CD@aol.com> hi everybody - i'm trying to send the ref for our acid fast for lipofuscin to someone - on the stain card all i have is the name of the book "laboratory methods in histotechnology" page 219 - the book itself is long gone apparently. i see there is an afip edited edition by the same title by edna b. prophet - if anyone has a copy of this book and can check to see if AF for lipofuscins is on page 219 i'd be forever grateful!!! Cheryl Cheryl Cross, DVM, Dipl. ACVP Researcher University Corporation for Atmospheric Research College of Veterinary Medicine University of Tennessee Department of Pathology 2407 River Drive, Room A201 Knoxville, TN 37996-4542 (423) 967-2724 fax: 865-974-5616 ccross@ucar.edu On Sep 24, 2007, at 2:02 PM, Shirley PHUA wrote: > I will be out of the office from 24-09-2007 to 25-09-2007. > > I'll be away on 24 Sep 2007 (Monday) afternoon. I'll be back on 24 > Sep > 2007 (Tuesday). > > Pathologists: > I will process your requests when I return. If urgent, please > forward your > email to Henry_Kyaw@hsa.gov.sg > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ryaskovich <@t> dir.nidcr.nih.gov Mon Sep 24 14:30:52 2007 From: ryaskovich <@t> dir.nidcr.nih.gov (Yaskovich, Ruth A (NIH/NIDCR) [E]) Date: Mon Sep 24 14:30:58 2007 Subject: [Histonet] biblio help? laboratory methods in histotechnology.... In-Reply-To: <157CA2E8-F881-47CB-9F1A-A2F07BA3D6CD@aol.com> References: <157CA2E8-F881-47CB-9F1A-A2F07BA3D6CD@aol.com> Message-ID: Cheryl, Yes it is. Ruth Yaskovich -----Original Message----- From: Cheryl Cross [mailto:ccross6032@aol.com] Sent: Monday, September 24, 2007 3:08 PM To: histonet Subject: [Histonet] biblio help? laboratory methods in histotechnology.... hi everybody - i'm trying to send the ref for our acid fast for lipofuscin to someone - on the stain card all i have is the name of the book "laboratory methods in histotechnology" page 219 - the book itself is long gone apparently. i see there is an afip edited edition by the same title by edna b. prophet - if anyone has a copy of this book and can check to see if AF for lipofuscins is on page 219 i'd be forever grateful!!! Cheryl Cheryl Cross, DVM, Dipl. ACVP Researcher University Corporation for Atmospheric Research College of Veterinary Medicine University of Tennessee Department of Pathology 2407 River Drive, Room A201 Knoxville, TN 37996-4542 (423) 967-2724 fax: 865-974-5616 ccross@ucar.edu On Sep 24, 2007, at 2:02 PM, Shirley PHUA wrote: > I will be out of the office from 24-09-2007 to 25-09-2007. > > I'll be away on 24 Sep 2007 (Monday) afternoon. I'll be back on 24 > Sep > 2007 (Tuesday). > > Pathologists: > I will process your requests when I return. If urgent, please > forward your > email to Henry_Kyaw@hsa.gov.sg > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ccross6032 <@t> aol.com Mon Sep 24 14:32:23 2007 From: ccross6032 <@t> aol.com (Cheryl Cross) Date: Mon Sep 24 14:32:47 2007 Subject: [Histonet] biblio help? laboratory methods in histotechnology.... In-Reply-To: References: <157CA2E8-F881-47CB-9F1A-A2F07BA3D6CD@aol.com> Message-ID: thanks everybody for the quick help, i really appreciate it! Cheryl Cross, DVM, Dipl. ACVP Researcher University Corporation for Atmospheric Research College of Veterinary Medicine University of Tennessee Department of Pathology 2407 River Drive, Room A201 Knoxville, TN 37996-4542 (423) 967-2724 fax: 865-974-5616 ccross@ucar.edu On Sep 24, 2007, at 3:30 PM, Yaskovich, Ruth A (NIH/NIDCR) [E] wrote: > Cheryl, > Yes it is. > Ruth Yaskovich > > -----Original Message----- > From: Cheryl Cross [mailto:ccross6032@aol.com] > Sent: Monday, September 24, 2007 3:08 PM > To: histonet > Subject: [Histonet] biblio help? laboratory methods in > histotechnology.... > > hi everybody - > > i'm trying to send the ref for our acid fast for lipofuscin to > someone - on the stain card all i have is the name of the book > "laboratory methods in histotechnology" page 219 - the book itself is > long gone apparently. i see there is an afip edited edition by the > same title by edna b. prophet - if anyone has a copy of this book and > can check to see if AF for lipofuscins is on page 219 i'd be forever > grateful!!! > > Cheryl > > Cheryl Cross, DVM, Dipl. ACVP > Researcher > University Corporation for Atmospheric Research > College of Veterinary Medicine > University of Tennessee Department of Pathology > 2407 River Drive, Room A201 > Knoxville, TN 37996-4542 > (423) 967-2724 > fax: 865-974-5616 > ccross@ucar.edu > > > > > > On Sep 24, 2007, at 2:02 PM, Shirley PHUA wrote: > >> I will be out of the office from 24-09-2007 to 25-09-2007. >> >> I'll be away on 24 Sep 2007 (Monday) afternoon. I'll be back on 24 >> Sep >> 2007 (Tuesday). >> >> Pathologists: >> I will process your requests when I return. If urgent, please >> forward your >> email to Henry_Kyaw@hsa.gov.sg >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nfournier <@t> sasktel.net Mon Sep 24 15:51:58 2007 From: nfournier <@t> sasktel.net (N Fournier) Date: Mon Sep 24 15:52:06 2007 Subject: [Histonet] DPX and Entellan mounting medium Message-ID: Hi everyone, I appreciate the helpful suggestions I received on a previous question. I was asked by a colleague to post a question to the group regarding if it was alright to use nail polish in addition to DPX or Entellan Mounting medium to seal slides. They noticed that the slides appeared fine initially (i.e., minimal air bubbles etc) but over a period of time (several weeks) large bubbles formed that surrounded the tissue causing it to dry out. Has anyone encountered similar problems. The tissue is 40 to 60 micron thick fixed coronal rat brain sections mounted on gelatin subbed and sometimes Fisher Superfrost Plus Slides. Sections are often stained immunohistochemically using the free-floating method. I unfortunately do not know the thickness of the coverslip, but I would imagine it is pretty standard guage. At first, I thought the problem might be with excess xylene on the slide dissolving the mounting medium or that that not enough was applied. From what I seen I do not believe this is the case but I could be wrong. What they do is run slides through alcohols and xylene. The slides are drained slightly on a Kim Wipe in order to remove some of the residual xylene and then mounting medium and coverslip is quickly applied. Air bubbles are removed by gently pressing them out of the slide. Would this method of applying nail polish reduce the occurrence of this problem? Are there any alternative suggestions? We appreciate your help, Neil Fournier From rjbuesa <@t> yahoo.com Mon Sep 24 16:22:11 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Sep 24 16:22:17 2007 Subject: [Histonet] DPX and Entellan mounting medium In-Reply-To: Message-ID: <885768.33370.qm@web61215.mail.yahoo.com> Neil: I don't think the nail polish is at all necessary. As long as the coverslip is large enough (larger than the section), is set flat over the section and let dry, they should be OK. IF the mounting medium is too diluted and the section too thick, the mounting medium will start to "retreat" from the edge into the section, and is likely that air bubbles will appear. You will need thcker mounting medium, less solvent in the mounting medium. And you will also need patiente, hardening of thick amounts of munting medium takes, at least, 2 weeks. If you have to handle the slides, do it gently and never try to move the coverslip. Once it is solidified you will not need any additional sealant. Ren? J. N Fournier wrote: Hi everyone, I appreciate the helpful suggestions I received on a previous question. I was asked by a colleague to post a question to the group regarding if it was alright to use nail polish in addition to DPX or Entellan Mounting medium to seal slides. They noticed that the slides appeared fine initially (i.e., minimal air bubbles etc) but over a period of time (several weeks) large bubbles formed that surrounded the tissue causing it to dry out. Has anyone encountered similar problems. The tissue is 40 to 60 micron thick fixed coronal rat brain sections mounted on gelatin subbed and sometimes Fisher Superfrost Plus Slides. Sections are often stained immunohistochemically using the free-floating method. I unfortunately do not know the thickness of the coverslip, but I would imagine it is pretty standard guage. At first, I thought the problem might be with excess xylene on the slide dissolving the mounting medium or that that not enough was applied. From what I seen I do not believe this is the case but I could be wrong. What they do is run slides through alcohols and xylene. The slides are drained slightly on a Kim Wipe in order to remove some of the residual xylene and then mounting medium and coverslip is quickly applied. Air bubbles are removed by gently pressing them out of the slide. Would this method of applying nail polish reduce the occurrence of this problem? Are there any alternative suggestions? We appreciate your help, Neil Fournier _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Check out the hottest 2008 models today at Yahoo! Autos. From naje1972 <@t> yahoo.com Mon Sep 24 22:34:20 2007 From: naje1972 <@t> yahoo.com (cynthia haynes) Date: Mon Sep 24 22:34:26 2007 Subject: [Histonet] Vendors welcomed Message-ID: <963045.89523.qm@web33011.mail.mud.yahoo.com> This message is to the vendors and manufacturers of Microtomes (automatic and rotary), water baths,H&E stainers, embedding units, Tissue processors I need quotes on all of these pieces of equipment. I am in the process of starting a new Histology laboratory and have been given the go ahead on the purchase of new equipment. I would also like prices on slides, coverslips, permount, slide folders, slide racks, Histology consumables in general. I can be reached at this email address or you may call me at 312-919-9887. I would like to receive these quotes no later than Friday of this week. Thank you, Cynthia Haynes H.T. From brucea <@t> unimelb.edu.au Tue Sep 25 02:50:41 2007 From: brucea <@t> unimelb.edu.au (Bruce Abaloz) Date: Tue Sep 25 02:51:05 2007 Subject: [Histonet] Mammary Epithelial cells & Milk Proteins Message-ID: Dear all, We are wanting to do H & E, PAS for Glycogen & Oil Red-O stains on Wallaby/Kangaroo mammary glands. Aside from these stains, we need to demonstrate 'Milk Protein/s' & am hoping that the wisdom of a colleague on Histonet will advise us.....what stain would anyone recommend?? Thanks & we appreciate all/any replies. Cheers..... Sonja Mailer & Bruce in OZ -- BRUCE ABALOZ PH:61383446282 HISTOLOGIST FAX:61383447909 DEPT. of ZOOLOGY EMAIL: brucea@unimelb.edu.au THE UNIVERSITY Of MELBOURNE. VICTORIA. AUSTRALIA 3010 Nobody Can Make You Feel Inferior Without YOUR Permission - Eleanor Roosevelt DANCE LIKE NO-ONE'S WATCHING Q: What's a specimen? A: An Italian astronaut. From doug <@t> ppspath.com Tue Sep 25 09:58:30 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue Sep 25 08:58:57 2007 Subject: [Histonet] Job Opening (SC) Message-ID: Professional Pathology Services, PC is one of the regions fastest growing Pathology laboratories located in sunny Columbia, South Carolina. We have a fully automated state-of-the-art histology laboratory that includes. Milestone Pathos processor Milestone Histos 3 processor (to be added 10/07) 5 conventional processors (Leica and Sakura) Ventana Benchmark XT IHC Ventana NexES special stainer Ventana Symphony H&E stainer/coverslipper Leica slide and cassette printers Leica stainer and coverslipper Updated microtomes and so much more. Come join our fun and exciting team of healthcare professionals. We are seeking a full-time certified histology technician (HT). ASCP certified w/1 or more years of experience in Histology. New Grads welcome to apply. This is an evening shift position (hours negotiable). Professional Pathology Services offers -Competitive salary -Relocation package -Sign-on bonus -Excellent working conditions www.ppspath.com Email resume to doug@ppspath.com Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. From Jonathan.Arzt <@t> ARS.USDA.GOV Tue Sep 25 09:08:30 2007 From: Jonathan.Arzt <@t> ARS.USDA.GOV (Arzt, Jonathan) Date: Tue Sep 25 09:10:06 2007 Subject: [Histonet] BIODEFENSE (HT/HTL) POSITION AVAILABLE (NY/CT) Message-ID: <6B4AF69268EFAE4C80A786BA99F7A6670152FFBA@MD-MAIL-01.ARSNET.ARS.USDA.GOV> BIODEFENSE (HT/HTL) POSITION AVAILABLE (NY/CT) A unique, full-time, permanent histotech. position will soon be available and advertised by the pathology division of the USDA, Animal Research Service on Plum Island, NY. The position will be offered at the grades of GS-7/9/11 conferring salary of $39K-$76K commensurate with experience and training. Benefits and promotion potential are excellent. Licensed HT and HTL preferred, but unlicensed candidates with appropriate experience will be considered and are encouraged to apply. The primary mission of the research group is investigation of veterinary diseases of potential threat to US agriculture interests. Currently the greatest emphases are directed towards characterizing the pathogenesis of foot-and-mouth disease and classical swine fever. The Plum Island Animal Disease Center is located off the East end of Long Island, NY. Transport to and from the island is provided free to employees aboard government-operated ferries servicing Orient, NY and Old Saybrook, CT. Rural and suburban communities are abundant near both the NY and CT sides of the ferries with access to scenic beaches and vineyards. New York City and Boston are within 2-3hrs drive. Activities will be varied but will include conventional histotech work with paraffin-embedded and frozen tissues, immunohistochemistry, in-situ hybridization, confocal microscopy, and assistance with animal necropsies and sample collection. The position will be advertised at: www.usajobs.com The exact date of posting is not known, but should be within the next 2 weeks. For more information, email to: Jonathan.Arzt@ARS.USDA.GOV If applying, please email to above address directly in addition to filing the application through USAJOBS. Note: this is an informational notice only and does not constitute an official advertisement of any position. From CIngles <@t> uwhealth.org Tue Sep 25 10:49:09 2007 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Tue Sep 25 10:49:21 2007 Subject: [Histonet] Re: Formaldehyde - Carcinogen? References: <407F05A128805F4C879A33DBA32E618E0189507B@TRFT-EX01.xRothGen.nhs.uk> Message-ID: <08A0A863637F1349BBFD83A96B27A50A12007D@uwhis-xchng3.uwhis.hosp.wisc.edu> I don't know. Up in the main lab where we got everything including limbs. I enjoyed observing (sorry didn't actually do the cutting-up/grossing). I have learned so much. I believe it has helped me become a better tech. I don't just see bits of tissue in paraffin. I understand the whole process better including how various disease processes affect different organs. I was always particularly facinated whenever a Teratoma would come in. Interesting to see what happens when something goes wrong in the body. I do some grossing now once in a while, but it is only skin biopsies with the occasional excision. It gets really boring when you may have 100+ specimens and they are all the same thing. :p Claire Ingles UW Hospital & Clinics Madison WI ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Marshall Terry Dr,Consultant Histopathologist Sent: Mon 9/24/2007 10:21 AM To: Marshall Terry Dr,Consultant Histopathologist; Cheri Miller; Kemlo Rogerson; Robert Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Formaldehyde - Carcinogen? Rene has privately advised me that cut-up = grossing on the other side of the pond. Whatever, both are as terms go, pretty gross. Does anybody actually enjoy it? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: 24 September 2007 15:47 To: Cheri Miller; Kemlo Rogerson; Robert Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Formaldehyde - Carcinogen? That's a great post to read first after getting back from an 1 hr 45 mins cut-up! :-) Terry -----Original Message----- From: Cheri Miller [mailto:cmiller@physlab.com] Sent: 24 September 2007 15:11 To: Marshall Terry Dr, Consultant Histopathologist; 'Kemlo Rogerson'; 'Robert Richmond'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Formaldehyde - Carcinogen? That is due to the fact that most pathologists don't due grossing beyond their Pathology Internship. Every path that I know has a rare exposure to formalin. It's the PA's, dieners and techs that are exposed. Just my 2 cents no flaming please I am sensitive. :) Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Monday, September 24, 2007 6:11 AM To: Kemlo Rogerson; Robert Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Formaldehyde - Carcinogen? Formalin. Psychiatrists. Terry -----Original Message----- From: Kemlo Rogerson [mailto:Kemlo.Rogerson@waht.swest.nhs.uk] Sent: 24 September 2007 08:57 To: Marshall Terry Dr, Consultant Histopathologist; Robert Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Formaldehyde - Carcinogen? "To date there has been no information published to suggest that pathologists' causes of death are different from those of other American physicians." Over here (UK) pathologists have the second highest suicide rate. This suggests that they haven't had time to get the long term effects of formalin:-) Terry - equally pickled. Terry was it the formalin that caused you to be so 'pickled' or another type of fixative? Who has the highest suicide rate? I don't actually know of any Pathologists that has committed suicide, only a few whose suicide was professional. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Loneliness Is An island In The middle Of A sea of people --R.R. (age 11) This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gvdobbin <@t> ihis.org Tue Sep 25 11:32:52 2007 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Tue Sep 25 11:33:19 2007 Subject: [Histonet] Potassium Ferrocyanide Question Message-ID: Hi Folks, Chemistry is not my forte for sure, so a little help from the chemistry gurus would be greatly appreciated. I purchased potassium ferrocyanide trihydrate for use in our Perl's stain. The bottle that is almost empty lists the contents as K4Fe(CN)6-3H2O with a molecular wt of 422.39. The new bottle (different supplier) uses the same name for the chemical ("potassium ferrocyanide trihydrate") but the formula is listed as FeK4N6-3H2O and the F.W. is 356.7. Can someone remind me what the "F" in F.W. stands for? And did I buy the right chemical? Both are ACS grade chemicals. Thank you. Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ayant pour objet une personne ou un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer compl?tement de votre syst?me informatique. From Jessica.Vacca <@t> HCAhealthcare.com Tue Sep 25 11:41:03 2007 From: Jessica.Vacca <@t> HCAhealthcare.com (Vacca Jessica) Date: Tue Sep 25 11:41:11 2007 Subject: [Histonet] CPT code for finger nails using potassium hydroxide Message-ID: <41E16A15CE78374EA45B57E0F94339B802D39B09@ORLEV01.hca.corpad.net> I have a question regarding the CPT charges for using KOH for softening of finger nails. Do you charge a decal charge of 88311 or can you not charge anything? Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Dr. Brandon Fl 33511 (813) 571-5193 or (813) 681-5551 ext 2454 Jessica.Vacca@hcahealthcare.com From JWEEMS <@t> sjha.org Tue Sep 25 11:51:40 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Tue Sep 25 11:51:59 2007 Subject: [Histonet] CPT code for finger nails using potassium hydroxide In-Reply-To: <41E16A15CE78374EA45B57E0F94339B802D39B09@ORLEV01.hca.corpad.net> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F3CBC@sjhaexc02.sjha.org> No CPT code for this, unfortunately. Doesn't make sense there would be one for decal and not this, does it? Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Vacca Jessica Sent: Tuesday, September 25, 2007 12:41 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] CPT code for finger nails using potassium hydroxide I have a question regarding the CPT charges for using KOH for softening of finger nails. Do you charge a decal charge of 88311 or can you not charge anything? Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Dr. Brandon Fl 33511 (813) 571-5193 or (813) 681-5551 ext 2454 Jessica.Vacca@hcahealthcare.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From rjbuesa <@t> yahoo.com Tue Sep 25 11:57:22 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Sep 25 11:57:28 2007 Subject: [Histonet] Potassium Ferrocyanide Question In-Reply-To: Message-ID: <662850.13258.qm@web61221.mail.yahoo.com> Greg: "F.W." is the same as "Molecular Weight", and stands for "Formula Weight" so if the manufacturer recognizes a different "FW" or "MW", something is not "kosher". Also check because you wrote two different composition formulas for the 2 products, the correct being the one you wrote first. ren? J. Greg Dobbin wrote: Hi Folks, Chemistry is not my forte for sure, so a little help from the chemistry gurus would be greatly appreciated. I purchased potassium ferrocyanide trihydrate for use in our Perl's stain. The bottle that is almost empty lists the contents as K4Fe(CN)6-3H2O with a molecular wt of 422.39. The new bottle (different supplier) uses the same name for the chemical ("potassium ferrocyanide trihydrate") but the formula is listed as FeK4N6-3H2O and the F.W. is 356.7. Can someone remind me what the "F" in F.W. stands for? And did I buy the right chemical? Both are ACS grade chemicals. Thank you. Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 --------------------------------- Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. From rjbuesa <@t> yahoo.com Tue Sep 25 12:00:39 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Sep 25 12:00:48 2007 Subject: [Histonet] CPT code for finger nails using potassium hydroxide In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F3CBC@sjhaexc02.sjha.org> Message-ID: <360416.16716.qm@web61216.mail.yahoo.com> There is no CPT code for KOH on nails and probably the cause is: 1- not averybody soften nails before processing them; 2- some HTs just "soften" nails while sectioning them, or 3- is a procedure that takes only 30 min. with 10% KOH. Regardless, it is another step and I think there should be a CPT code for it. Ren? J. --------------------------------- Shape Yahoo! in your own image. Join our Network Research Panel today! From doug <@t> ppspath.com Tue Sep 25 13:03:22 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue Sep 25 12:04:00 2007 Subject: {SPAM?} [Histonet] Potassium Ferrocyanide Question In-Reply-To: Message-ID: Formula weight is commonly abbreviated FW. The formula weight of a substance is the sum of the atomic weights of each atom in the chemical formula. When the chemical formula equals the molecular formula, then the formula weight is also called the molecular weight. The abbreviation commonly used for molecular weight is MW. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Greg Dobbin Sent: Tuesday, September 25, 2007 11:33 AM To: Histonet@lists.utsouthwestern.edu Subject: {SPAM?} [Histonet] Potassium Ferrocyanide Question Hi Folks, Chemistry is not my forte for sure, so a little help from the chemistry gurus would be greatly appreciated. I purchased potassium ferrocyanide trihydrate for use in our Perl's stain. The bottle that is almost empty lists the contents as K4Fe(CN)6-3H2O with a molecular wt of 422.39. The new bottle (different supplier) uses the same name for the chemical ("potassium ferrocyanide trihydrate") but the formula is listed as FeK4N6-3H2O and the F.W. is 356.7. Can someone remind me what the "F" in F.W. stands for? And did I buy the right chemical? Both are ACS grade chemicals. Thank you. Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ayant pour objet une personne ou un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer compl?tement de votre syst?me informatique. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From llewllew <@t> shaw.ca Tue Sep 25 12:54:19 2007 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Tue Sep 25 12:55:41 2007 Subject: [Histonet] Potassium Ferrocyanide Question References: Message-ID: <002501c7ff9d$18fed250$05024246@yourlk4rlmsu> Potassium ferrocyanide is usually given the formula you first noted. However, my Merck Index also gives the formula C6FeK4N6 with a MW of 368.34 (you may have a typo and left out the C6). The differences in MW may be due to including or not including the 3H2O. Just do a test Perls' stain on an iron control section. If they are the same then don't worry about it. Bryan Llewellyn ----- Original Message ----- From: "Greg Dobbin" To: Sent: Tuesday, September 25, 2007 9:32 AM Subject: [Histonet] Potassium Ferrocyanide Question > Hi Folks, > Chemistry is not my forte for sure, so a little help from the chemistry > gurus would be greatly appreciated. > > I purchased potassium ferrocyanide trihydrate for use in our Perl's > stain. The bottle that is almost empty lists the contents as > K4Fe(CN)6-3H2O with a molecular wt of 422.39. > > The new bottle (different supplier) uses the same name for the chemical > ("potassium ferrocyanide trihydrate") but the formula is listed as > FeK4N6-3H2O and the F.W. is 356.7. > > Can someone remind me what the "F" in F.W. stands for? And did I buy > the right chemical? Both are ACS grade chemicals. > > Thank you. > Greg > > Greg Dobbin, R.T. > Chief Technologist, Histology Lab > Dept. of Laboratory Medicine, > Queen Elizabeth Hospital, > P.O. Box 6600 > Charlottetown, PE C1A 8T5 > Phone: (902) 894-2337 > Fax: (902) 894-2385 > > > Statement of Confidentiality > This message (including attachments) may contain confidential or > privileged information intended for a specific individual or organization. > If you have received this communication in error, please notify the sender > immediately. If you are not the intended recipient, you are not > authorized to use, disclose, distribute, copy, print or rely on this > email, and should promptly delete this email from your entire computer > system. > > D?claration de confidentialit? > Le pr?sent message (y compris les annexes) peut contenir des > renseignements confidentiels ayant pour objet une personne ou un organisme > particulier. Si vous avez re?u la pr?sente communication par erreur, > veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le > destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, > distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et > vous devriez l'effacer compl?tement de votre syst?me informatique. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Shirley.Chu <@t> moldev.com Tue Sep 25 13:25:35 2007 From: Shirley.Chu <@t> moldev.com (Chu, Shirley) Date: Tue Sep 25 13:28:39 2007 Subject: [Histonet] Laser Capture Microdissection Web Seminar Message-ID: An Arcturus LCM webinar will be presented on Tues, Oct 16th by Anna Pecherskaya of the Fox Chase Cancer Center. The title of her talk is: "Practical Applications of Laser Capture Microdissection". There is no cost to attend this webinar. An abstract of this presentation and registration information can be found on the following website: http://www.moleculardevices.com/pages/webinar_arcturus.html Shirley Chu Applications Scientist, Arcturus LCM Products Molecular Devices (now a part of MDS Analytical Technologies) 510-675-6260 | www.moleculardevices.com From JGREWE <@t> OhioHealth.com Tue Sep 25 15:01:09 2007 From: JGREWE <@t> OhioHealth.com (JGREWE@OhioHealth.com) Date: Tue Sep 25 15:01:25 2007 Subject: [Histonet] Jacquelyn Grewe/Staff/OhioHealth is out of the office . Message-ID: I will be out of the office starting 09/24/2007 and will not return until 09/30/2007. I will respond to your message when I return. Thanks, Jackie From jnocito <@t> satx.rr.com Tue Sep 25 16:18:57 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Sep 25 16:19:13 2007 Subject: [Histonet] CPT code for finger nails using potassium hydroxide References: <360416.16716.qm@web61216.mail.yahoo.com> Message-ID: <000c01c7ffb9$b03da0d0$0202a8c0@yourxhtr8hvc4p> I have to agree with Rene. There is a CPT for decals, there should be one for nails. And this is coming from Joe the Toe. ----- Original Message ----- From: "Rene J Buesa" To: "Weems, Joyce" ; "Vacca Jessica" ; Sent: Tuesday, September 25, 2007 12:00 PM Subject: RE: [Histonet] CPT code for finger nails using potassium hydroxide > There is no CPT code for KOH on nails and probably the cause is: > 1- not averybody soften nails before processing them; > 2- some HTs just "soften" nails while sectioning them, or > 3- is a procedure that takes only 30 min. with 10% KOH. > Regardless, it is another step and I think there should be a CPT code for > it. > Ren? J. > > > > > > > > > --------------------------------- > Shape Yahoo! in your own image. Join our Network Research Panel today! > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dellav <@t> musc.edu Tue Sep 25 16:38:05 2007 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Tue Sep 25 16:39:43 2007 Subject: [Histonet] Potassium Ferrocyanide Question In-Reply-To: References: Message-ID: <7F6B678A32B0564196138E6B3101996ABF208B@EVS1.clinlan.local> Greg, In comparing the two formulas you typed I don't see the cyanide (CN) in the second formula so I'm sort of confused. Is that a typo on your part ? to answer your question more specifically, if the chemicals were the same the formula weight (FW) should be the same, and clearly your new chemical is quite different from the first. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Greg Dobbin Sent: Tuesday, September 25, 2007 12:33 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Potassium Ferrocyanide Question Hi Folks, Chemistry is not my forte for sure, so a little help from the chemistry gurus would be greatly appreciated. I purchased potassium ferrocyanide trihydrate for use in our Perl's stain. The bottle that is almost empty lists the contents as K4Fe(CN)6-3H2O with a molecular wt of 422.39. The new bottle (different supplier) uses the same name for the chemical ("potassium ferrocyanide trihydrate") but the formula is listed as FeK4N6-3H2O and the F.W. is 356.7. Can someone remind me what the "F" in F.W. stands for? And did I buy the right chemical? Both are ACS grade chemicals. Thank you. Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ayant pour objet une personne ou un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer compl?tement de votre syst?me informatique. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Tue Sep 25 17:43:30 2007 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Sep 25 17:44:06 2007 Subject: [Histonet] Potassium Ferrocyanide Question Message-ID: FW means Formula weight. It is usually the same as the molecular weight. BUT According to the formulas you have recorded in your email, the second is missing a few carbons ie: K4Fe(CN)6-3H2O with a molecular wt of 422.39 FeK4N6-3H2O and the F.W. is 356.7. Is this correct? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Greg Dobbin Sent: Wednesday, 26 September 2007 2:33 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Potassium Ferrocyanide Question Hi Folks, Chemistry is not my forte for sure, so a little help from the chemistry gurus would be greatly appreciated. I purchased potassium ferrocyanide trihydrate for use in our Perl's stain. The bottle that is almost empty lists the contents as K4Fe(CN)6-3H2O with a molecular wt of 422.39. The new bottle (different supplier) uses the same name for the chemical ("potassium ferrocyanide trihydrate") but the formula is listed as FeK4N6-3H2O and the F.W. is 356.7. Can someone remind me what the "F" in F.W. stands for? And did I buy the right chemical? Both are ACS grade chemicals. Thank you. Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ayant pour objet une personne ou un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer compl?tement de votre syst?me informatique. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From ernestinemiddleton <@t> yahoo.ca Tue Sep 25 19:46:21 2007 From: ernestinemiddleton <@t> yahoo.ca (Ernestine Middleton) Date: Tue Sep 25 19:46:26 2007 Subject: [Histonet] Position opening Message-ID: <877501.47437.qm@web51511.mail.re2.yahoo.com> Hi: Montefiore Medical Center, Bronx, NY is seeking Mohs technologist. Must have NYS license. Will accept a parttime personnel until and full time person can be found. Please e-mail resume to emiddlet@montefiore.org. We are still looking for evening Histology supervirsor. Ernestine Middleton Montefiore Medical Center Bronx, NY 718-920-4157 718-547-1920 fax emiddlet@montefiore.org --------------------------------- Ask a question on any topic and get answers from real people. Go to Yahoo! Answers. From ombadda3 <@t> gmail.com Wed Sep 26 06:20:12 2007 From: ombadda3 <@t> gmail.com (K M) Date: Wed Sep 26 06:24:45 2007 Subject: [Histonet] How to choose a topic of a master degree thesis? Message-ID: Glad to be here.This is my very post here.Iam preparing to do a master degree in anatomical pathology and I am little confused how can I choose a topic to work on my thesis? My supervisor said to me go to GOOGLE but google made me more confused! By the way I am in Egypt and interest in immunohistochemistry studies .but which organ I choose ?what disease I choose??? Any comment. From godsgalnow <@t> aol.com Wed Sep 26 07:27:48 2007 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Wed Sep 26 07:27:57 2007 Subject: [Histonet] wax on the rug Message-ID: <8C9CE4FC8B1ABE2-2CC-579@FWM-D01.sysops.aol.com> Hello 'Netters, Anyone out there know how to get paraffin out of the rug?? You know, from tracking it into and down the hallway on your feet? I do know that they have the sticky tape that you can put on the floor of the lab before the carpet begins to take the wax off your shoes, but the owner of the lab thinks it looks horrible and doesn't like it. Thanks in advance.... Roxanne ________________________________________________________________________ Email and AIM finally together. You've gotta check out free AOL Mail! - http://mail.aol.com From b-frederick <@t> northwestern.edu Wed Sep 26 07:32:21 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Sep 26 07:32:12 2007 Subject: [Histonet] CPT code for finger nails using potassium hydroxide In-Reply-To: <000c01c7ffb9$b03da0d0$0202a8c0@yourxhtr8hvc4p> Message-ID: <004801c80039$4c5601e0$d00f7ca5@lurie.northwestern.edu> All- I would think decal is decal no matter the means to do so. We charge decal even it's a surface decal. We use Nair or Neet to soften nails. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Tuesday, September 25, 2007 4:19 PM To: Rene J Buesa; Weems, Joyce; Vacca Jessica; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] CPT code for finger nails using potassium hydroxide I have to agree with Rene. There is a CPT for decals, there should be one for nails. And this is coming from Joe the Toe. ----- Original Message ----- From: "Rene J Buesa" To: "Weems, Joyce" ; "Vacca Jessica" ; Sent: Tuesday, September 25, 2007 12:00 PM Subject: RE: [Histonet] CPT code for finger nails using potassium hydroxide > There is no CPT code for KOH on nails and probably the cause is: > 1- not averybody soften nails before processing them; > 2- some HTs just "soften" nails while sectioning them, or > 3- is a procedure that takes only 30 min. with 10% KOH. > Regardless, it is another step and I think there should be a CPT code for > it. > Ren? J. > > > > > > > > > --------------------------------- > Shape Yahoo! in your own image. Join our Network Research Panel today! > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Sep 26 07:35:33 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 26 07:35:37 2007 Subject: [Histonet] How to choose a topic of a master degree thesis? In-Reply-To: Message-ID: <260472.4026.qm@web61213.mail.yahoo.com> KM Why don't you try a comparison of melanin precursors identifiers? There are many antibodies you can use, and you can make comparisons about their "early detection" efficacy, for which you can use different types of lesions, from nevus to pre-melanomas to melanomas correlating the reaction intensities with the antibodies dilution rates.Under separate cover I am sending you the abstract of a paper on the subject and the results of some experiments on the topic I did in 2002 You should consult with your pathologist to select the specimens for your study. Ren? J. K M wrote: Glad to be here.This is my very post here.Iam preparing to do a master degree in anatomical pathology and I am little confused how can I choose a topic to work on my thesis? My supervisor said to me go to GOOGLE but google made me more confused! By the way I am in Egypt and interest in immunohistochemistry studies .but which organ I choose ?what disease I choose??? Any comment. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Got a little couch potato? Check out fun summer activities for kids. From doug <@t> ppspath.com Wed Sep 26 08:43:47 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Wed Sep 26 07:44:15 2007 Subject: {SPAM?} [Histonet] wax on the rug In-Reply-To: <8C9CE4FC8B1ABE2-2CC-579@FWM-D01.sysops.aol.com> Message-ID: Roxanne, I am not sure if you want to go to the trouble but I have used this method... 1. Obtain an iron and a brown grocery bag. 2. Place one layer of the paper bag over the spot with wax. 3. With your iron on medium high, and iron over the paper bag over the place with wax. 4. Move the bag around if necessary. The wax will warm up and stick to the grocery bag. 5. Be diligent until all wax is gone. You may need more than one paper bag, depending on the size of the hardened wax. 6. Use a lower setting for delicate materials on the iron. I have also used paper towels in place of the grocery bag. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: Wednesday, September 26, 2007 7:28 AM To: histonet@lists.utsouthwestern.edu Subject: {SPAM?} [Histonet] wax on the rug Hello 'Netters, Anyone out there know how to get paraffin out of the rug?? You know, from tracking it into and down the hallway on your feet? I do know that they have the sticky tape that you can put on the floor of the lab before the carpet begins to take the wax off your shoes, but the owner of the lab thinks it looks horrible and doesn't like it. Thanks in advance.... Roxanne ________________________________________________________________________ Email and AIM finally together. You've gotta check out free AOL Mail! - http://mail.aol.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Wed Sep 26 07:47:00 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Wed Sep 26 07:51:01 2007 Subject: [Histonet] wax on the rug In-Reply-To: <8C9CE4FC8B1ABE2-2CC-579@FWM-D01.sysops.aol.com> References: <8C9CE4FC8B1ABE2-2CC-579@FWM-D01.sysops.aol.com> Message-ID: The way I do it at home is put a paper bag over the wax and iron the carpet through the paper bag. Set the iron on low! The wax should be absorbed by the paper bag and your carpet is saved. This is probably not a good idea for large areas, unless you have a lot of time. Emily -- as soon as you tolerate something, it becomes bearable, and before long it will become common --Israel Zangwill From JWEEMS <@t> sjha.org Wed Sep 26 07:54:06 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Sep 26 07:54:28 2007 Subject: [Histonet] wax on the rug In-Reply-To: <8C9CE4FC8B1ABE2-2CC-579@FWM-D01.sysops.aol.com> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F3CD6@sjhaexc02.sjha.org> Depending on how much is there, freeze it with freon and pop it up. If it is ground in, you might try ironing (not too hot!) over a brown grocery bag. Tacky mats for operating rooms are helpful in keeping the amount of paraffin down. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of godsgalnow@aol.com Sent: Wednesday, September 26, 2007 8:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] wax on the rug Hello 'Netters, Anyone out there know how to get paraffin out of the rug?? You know, from tracking it into and down the hallway on your feet? I do know that they have the sticky tape that you can put on the floor of the lab before the carpet begins to take the wax off your shoes, but the owner of the lab thinks it looks horrible and doesn't like it. Thanks in advance.... Roxanne ________________________________________________________________________ Email and AIM finally together. You've gotta check out free AOL Mail! - http://mail.aol.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From godsgalnow <@t> aol.com Wed Sep 26 08:26:04 2007 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Wed Sep 26 08:26:13 2007 Subject: [Histonet] Cytology Quality Improvement standards Message-ID: <8C9CE57EC453070-2CC-8A6@FWM-D01.sysops.aol.com> I am the Lab Supervisor for a private lab that does urine cytology, as well as the bladder biopsies and prostate biopsies.? We do FISH as well.? I am not a cytotech and we are trying to develop some QA/QI standards and guidelines.? Currently we do not have a QA program in place for our routine cytology, other than the Histo/Cyto Correlation because all screened slides are signed out by a Pathologist so we get 100 QA on them anyway. But, when I go back to do the Histo/Cyto Correlation, there are sometimes discrepancies.? For instance, the cytology will be negative, the FISH will be negative, but the bladder biopsies will show TCC.? So, in order to continue to stop doing the Correlation of these findings in a vacuum, we need some kind of standards as to what % discrepant diagnosis is acceptable.? How many false positives, false negatives, etc..can be expected. Does anyone have any insight on this and can shed some light on this for me?? Roxanne ________________________________________________________________________ Email and AIM finally together. You've gotta check out free AOL Mail! - http://mail.aol.com From Kemlo.Rogerson <@t> waht.swest.nhs.uk Wed Sep 26 08:32:27 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Sep 26 08:32:33 2007 Subject: [Histonet] How to choose a topic of a master degree thesis? Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222EDA5@wahtntex2.waht.swest.nhs.uk> Secret is to do something on something you enjoy. Don't try and do an MSc on something you think is a tad boring as it will get very, very boring!! I assume you have a Supervisor? Your Uni would have one PLUS you need one at your place of work; what does your supervisor say? Next try and find someone, like a Medic, that has a specific interest but no time to pursue it. If you try and come up with something that your organisation has no interest in then you will struggle. If you have a Research Department ask them, if you know of anyone carrying out research then ask them. Unfortunately immunocytochemistry interests me not at all so I can't help and if someone from the Forum suggests something then you will be in an academically sterile atmosphere, struggling by yourself. Why not carry out some immuncytochemistry on Mummies? Might get a grant and involved in a research programme, but I've no idea what you could look for in mummies or how you would get the tissue. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Loneliness Is An island In The middle Of A sea of people --R.R. (age 11) This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From ian.montgomery <@t> bio.gla.ac.uk Wed Sep 26 08:46:38 2007 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Wed Sep 26 08:46:46 2007 Subject: FW: [Histonet] wax on the rug Message-ID: <003501c80043$a99bd4b0$6424d182@IBLS.GLA.AC.UK> Roxanne, Brown wrapping paper and a hot iron, then repeat until the wax has been removed. Or, claim the insurance and buy a new one. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, G12 8QQ. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: 26 September 2007 13:28 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] wax on the rug Hello 'Netters, Anyone out there know how to get paraffin out of the rug?? You know, from tracking it into and down the hallway on your feet? I do know that they have the sticky tape that you can put on the floor of the lab before the carpet begins to take the wax off your shoes, but the owner of the lab thinks it looks horrible and doesn't like it. Thanks in advance.... Roxanne ________________________________________________________________________ Email and AIM finally together. You've gotta check out free AOL Mail! - http://mail.aol.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Sep 26 08:46:42 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 26 08:46:50 2007 Subject: [Histonet] Cytology Quality Improvement standards In-Reply-To: <8C9CE57EC453070-2CC-8A6@FWM-D01.sysops.aol.com> Message-ID: <747836.20548.qm@web61211.mail.yahoo.com> There is an interdepartmental CAP program, similar to the one for IHC, that you can subscribe to. The screeners and pathologists participate and the slides they send for interpretation are graded and this is one of the best and strongest QA existing programs. Also your pathologists should be the ones to answer the questions you have, specially those referring to ASCUS and false positives or negatives that should tend to be "0" Ren? J. godsgalnow@aol.com wrote: I am the Lab Supervisor for a private lab that does urine cytology, as well as the bladder biopsies and prostate biopsies.? We do FISH as well.? I am not a cytotech and we are trying to develop some QA/QI standards and guidelines.? Currently we do not have a QA program in place for our routine cytology, other than the Histo/Cyto Correlation because all screened slides are signed out by a Pathologist so we get 100 QA on them anyway. But, when I go back to do the Histo/Cyto Correlation, there are sometimes discrepancies.? For instance, the cytology will be negative, the FISH will be negative, but the bladder biopsies will show TCC.? So, in order to continue to stop doing the Correlation of these findings in a vacuum, we need some kind of standards as to what % discrepant diagnosis is acceptable.? How many false positives, false negatives, etc..can be expected. Does anyone have any insight on this and can shed some light on this for me?? Roxanne ________________________________________________________________________ Email and AIM finally together. You've gotta check out free AOL Mail! - http://mail.aol.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Boardwalk for $500? In 2007? Ha! Play Monopoly Here and Now (it's updated for today's economy) at Yahoo! Games. From gvdobbin <@t> ihis.org Wed Sep 26 09:12:59 2007 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Wed Sep 26 09:13:28 2007 Subject: [Histonet] wax on the rug Message-ID: Am I the only one who sees the humour in this subject line?!? :-) Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 >>> 9/26/2007 9:27 AM >>> Hello 'Netters, Anyone out there know how to get paraffin out of the rug?? You know, from tracking it into and down the hallway on your feet? I do know that they have the sticky tape that you can put on the floor of the lab before the carpet begins to take the wax off your shoes, but the owner of the lab thinks it looks horrible and doesn't like it. Thanks in advance.... Roxanne ________________________________________________________________________ Email and AIM finally together. You've gotta check out free AOL Mail! - http://mail.aol.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ayant pour objet une personne ou un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer compl?tement de votre syst?me informatique. From tkngflght <@t> yahoo.com Wed Sep 26 10:01:55 2007 From: tkngflght <@t> yahoo.com (Cheryl R. Kerry) Date: Wed Sep 26 10:01:47 2007 Subject: [Histonet] Rugs and safety--PLEASE READ In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F3CD6@sjhaexc02.sjha.org> Message-ID: <004001c8004e$2e4df850$6401a8c0@CHERYLSLAPTOP> When you utilize rugs and such, LISTEN to your staff!!! If they complain of tripping or having problems with the rugs buckling--LISTEN and DO SOMETHING!!! As you all know I run a temp agency and I've been a working tech for a long time. We work with labs that use rugs--and I've had them in labs I've managed. We taped the buckles to the floor or required the rental company to purchase industrial quality rugs to keep things safe. We changed the wheel on the chairs to reduce the lifting by rolling across the edges. All created a safer lab at very little cost. In one of our client labs the techs have been tripping and complaining about the hazard when at work. Unfortunately the hazard never filtered back to our office until it was too late. One of our techs tripped last night and ended up in an ambulance to the ER. She could've broken a hip the way she fell--it was horrible. This was not an unknown hazard for the lab or the techs--which creates a liability issue on top of the injury. If anyone brings a safety issue to you--please listen!! They aren't just complaining to complain. No one wants to be off of work for a preventable injury. Rugs create a safer, cleaner floor, but only when properly maintained. Thanks for listening--it was a really long night. She's going to be okay but it's going to be a sore couple of weeks. I'm sure I'm going to get flamed but if one less person is injured because of this--fine--bring it. Cheryl Cheryl R. Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab, one great tech at a time. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone From christiegowan <@t> msn.com Wed Sep 26 10:03:27 2007 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Wed Sep 26 10:03:36 2007 Subject: [Histonet] wax on the rug In-Reply-To: <8C9CE4FC8B1ABE2-2CC-579@FWM-D01.sysops.aol.com> Message-ID: I would check with a carpet cleaning company that specializes in commercial and industrial jobs. Perhaps they have a solvent based cleaner that removes the buildup of tracked paraffin. In the meantime I would have the hospital put down carpet mats that can be switched out and cleaned periodically. My 2 cents. Christie Gowan >From: godsgalnow@aol.com >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] wax on the rug >Date: Wed, 26 Sep 2007 08:27:48 -0400 > >Hello 'Netters, > > >Anyone out there know how to get paraffin out of the rug?? You know, from >tracking it into and down the hallway on your feet? > >I do know that they have the sticky tape that you can put on the floor of >the lab before the carpet begins to take the wax off your shoes, but the >owner of the lab thinks it looks horrible and doesn't like it. > >Thanks in advance.... > >Roxanne >________________________________________________________________________ >Email and AIM finally together. You've gotta check out free AOL Mail! - >http://mail.aol.com >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alaskagirl1950 <@t> yahoo.com Wed Sep 26 10:16:07 2007 From: alaskagirl1950 <@t> yahoo.com (Patricia Adams) Date: Wed Sep 26 10:16:13 2007 Subject: [Histonet] wax on the rug In-Reply-To: Message-ID: <354044.95362.qm@web52512.mail.re2.yahoo.com> A hospital that I worked for had their cleaning group use a steam cleaner to remove the paraffin from the carpets. We had two sets one to put down while the other was sent to be cleaned. Worked great. Patricia --- CHRISTIE GOWAN wrote: > I would check with a carpet cleaning company > that specializes in commercial > and industrial jobs. Perhaps they have a > solvent based cleaner that removes > the buildup of tracked paraffin. In the > meantime I would have the hospital > put down carpet mats that can be switched out > and cleaned periodically. My 2 > cents. > Christie Gowan > > > >From: godsgalnow@aol.com > >To: histonet@lists.utsouthwestern.edu > >Subject: [Histonet] wax on the rug > >Date: Wed, 26 Sep 2007 08:27:48 -0400 > > > >Hello 'Netters, > > > > > >Anyone out there know how to get paraffin out > of the rug?? You know, from > >tracking it into and down the hallway on your > feet? > > > >I do know that they have the sticky tape that > you can put on the floor of > >the lab before the carpet begins to take the > wax off your shoes, but the > >owner of the lab thinks it looks horrible and > doesn't like it. > > > >Thanks in advance.... > > > >Roxanne > >________________________________________________________________________ > >Email and AIM finally together. You've gotta > check out free AOL Mail! - > >http://mail.aol.com > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D ____________________________________________________________________________________ Pinpoint customers who are looking for what you sell. http://searchmarketing.yahoo.com/ From CarterK <@t> MedImmune.com Wed Sep 26 10:22:52 2007 From: CarterK <@t> MedImmune.com (Carter, Kendra) Date: Wed Sep 26 10:24:45 2007 Subject: [Histonet] GDP or GLP Documentation Training Message-ID: Could anyone recommend any GDP or GLP Documentation Training for the Mid-Atlantic area? Thank you. Kendra Leigh Carter MedImmune, Inc. One MedImmune Way Gaithersburg, MD 20878 PH: 301-398-4956 Fax: 301-398-9956 From liz <@t> premierlab.com Wed Sep 26 11:11:13 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Sep 26 11:11:20 2007 Subject: [Histonet] mycobacterium tuberculosis and fixation Message-ID: I'm posting this question since I need to research the viability of = mycobaterium tuberculosis in fixed tissue samples. We quite freqently = handle paraformaldehye fixed samples from guinea pigs and mice that have = been that infected with MTB, after fixation they are stored in 70% = alchol for weeks before I get them. I have culture data that shows that = these samples that I am receiving have no viable TB present. I'm = wondering if there is anyone out there that handles fixed TB samples = differently or are they considered non-infectious. There has been one = published study that did find viable bacteria in autopsy samples that = had been stored in 10% NBF so there is conflicting data. =20 =20 Our policy on these type of samples is that we only accept fixed tissue = samples, we do not work with any unfixed tissue samples. We do make = available for individuals that are grossing in the samples a 3M N95 = particulate respirator, wearing the respirator is not manditory and we = have run this procedure past our local OSHA person and they are fine = with it. =20 =20 The one concern I have is that we use a shop vac to suck up the paraffin = debris from the microtome and is this causing aerosolization. Any = comments are appreciated and thanks in advance. =20 Liz =20 Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 HYPERLINK "mailto:liz@premierlab.com"liz@premierlab.com HYPERLINK "http://www.premierlab.com/"www.premierlab.com =20 Ship to Address: =20 Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 =20 No virus found in this outgoing message. Checked by AVG Free Edition.=20 Version: 7.5.488 / Virus Database: 269.13.30/1030 - Release Date: = 9/25/2007 8:02 AM =20 From liz <@t> premierlab.com Wed Sep 26 11:43:33 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Sep 26 11:43:39 2007 Subject: [Histonet] GDP or GLP Documentation Training In-Reply-To: <393DFCC5BBE043E99F41D1CE0C8968B5@PremierLab.local> References: <393DFCC5BBE043E99F41D1CE0C8968B5@PremierLab.local> Message-ID: Kendra We subcontract out our QA to a local company here in Boulder and they = have also helped us with GLP training, etc. I would suspect that there = are companies that are local to you that deal with regulatory issues = that could help you out. Check to see if your state has a Bioscience = association or something similar, if they do they will list vendors that = can provide those types of support services. If you like I could = contact my guys here and see if they know anyone that is in your area. Liz=20 Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com =20 Ship to Address: =20 Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu = [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carter, = Kendra Sent: Wednesday, September 26, 2007 9:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GDP or GLP Documentation Training Could anyone recommend any GDP or GLP Documentation Training for the = Mid-Atlantic area? Thank you. =20 Kendra Leigh Carter MedImmune, Inc. One MedImmune Way Gaithersburg, MD 20878 =20 PH: 301-398-4956 Fax: 301-398-9956 =20 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG Free Edition.=20 Version: 7.5.488 / Virus Database: 269.13.30/1030 - Release Date: = 9/25/2007 8:02 AM =20 No virus found in this outgoing message. Checked by AVG Free Edition.=20 Version: 7.5.488 / Virus Database: 269.13.30/1030 - Release Date: = 9/25/2007 8:02 AM =20 From chengkaz <@t> uci.edu Wed Sep 26 11:54:31 2007 From: chengkaz <@t> uci.edu (Chengkang ZHANG) Date: Wed Sep 26 11:54:39 2007 Subject: [Histonet] Re: Maleic acid vs. Tris in in situ buffer Message-ID: <46FA8EC7.1020105@uci.edu> Dear All, I am doing DIG-labeled RNA in situ hybridizations and noticed that some protocols use MABT buffer (0.1M maleic acid, 0.15M NaCl, 0.1% Tween-20, pH 7.5) to dilute AP-conjugated anti-DIG antibody, while the others use TNT buffer (0.1M Tris, 0.15M NaCl, 0.1% Tween-20, pH7.5). The only difference between these two buffers is Maleic acid vs. Tris. I used Tris-based buffer for my Ab solution and it worked for me. I am just curious about the difference between maleic acid and Tris. Does anyone know if there is any advantage of choosing maleic acid over Tris? Thanks for your input. CK. ================================== Chengkang Zhang Ph.D. Room 357, MedSurge II 19182 Jamboree Rd, Department of Pharmacology University of California, Irvine Irvine, CA 92697-4625 Email: chengkaz@uci.edu Tel: (949)-824-1902 (lab) Fax: (949)-824-4855 ================================== From katia.catunda <@t> cipax.com.br Wed Sep 26 12:33:23 2007 From: katia.catunda <@t> cipax.com.br (=?iso-8859-1?Q?Ms._K=E1tia_Cristina_Catunda?=) Date: Wed Sep 26 12:33:14 2007 Subject: [Histonet] CD138 and Mycobacterium bovis In-Reply-To: Message-ID: Hi there, Does anybody has a clone recomendation for the antibody CD138?? We used to use clone MI15 from Dako but were informed that it was discontinued! And vendors for Mycobacterium bovis? antibody? Dako is not working well! Sincerely, Ms. K?tia Catunda Produ??o +55 12 3203-0612 (direto) +55 12 3203-0633 (PABX) www.cipax.com.br katia.catunda@cipax.com.br From Boneslides <@t> aol.com Wed Sep 26 13:26:08 2007 From: Boneslides <@t> aol.com (Boneslides@aol.com) Date: Wed Sep 26 13:28:27 2007 Subject: [Histonet] Embedding polyurethane Message-ID: Hello Histonetters!! Does anyone know if hexane can be used in place of acetone? I have a series of orthopedic specimens that contain a polyurethane implant that the researcher wants embedded in Spurr's and then rough cut and ground. I used my usual protocol, but the acetone caused the polyurethane to swell. The company did some research and was told that hexane could work and possibly something called IPA, as these are both considered non-solvents for polyurethane. I am not familiar with either of these substances. Any one have any insight?? Any help would be much appreciated! ~Diane Diane M. Mahovlic, HT, MLT(ASCP) Orthopedic Pathology & Biomaterials Laboratory Department of Anatomic Pathology The Cleveland Clinic Foundation 9500 Euclid Avenue- L30 Cleveland, Ohio 44195 216-444-0166 ************************************** See what's new at http://www.aol.com From judi.ford <@t> roche.com Wed Sep 26 13:36:18 2007 From: judi.ford <@t> roche.com (Ford, Judi) Date: Wed Sep 26 13:37:50 2007 Subject: [Histonet] Hydrogen Peroxide Question Message-ID: Hi Everyone, I wanted to post this for one of my co-workers. What percentage H2O2 is used to quench endogenous peroxidase in frozen section CD marker staining? how long? Thanks, Judi Ford Palo Alto, CA From mtarango <@t> nvcancer.org Wed Sep 26 13:52:03 2007 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Wed Sep 26 13:52:16 2007 Subject: [Histonet] How to choose a topic of a master degree thesis? In-Reply-To: Message-ID: <5AEC610C1CE02945BD63A395BA763EDE011B6E84@NVCIEXCH02.NVCI.org> Choose something that you can match up with some other assays. If you are going to do IHC pick a gene and use an antibody against the protein...and then come up with a good story about what it all means. Do some FISH, PCR and other stuff to support the IHC. Doing IHC alone would most likely make for a really boring paper. Mark Adam Tarango HT(ASCP) Histology & IHC Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 Direct Line (702) 822-5112 Mobile (702) 759-9229 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of K M Sent: Wednesday, September 26, 2007 4:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How to choose a topic of a master degree thesis? Glad to be here.This is my very post here.Iam preparing to do a master degree in anatomical pathology and I am little confused how can I choose a topic to work on my thesis? My supervisor said to me go to GOOGLE but google made me more confused! By the way I am in Egypt and interest in immunohistochemistry studies .but which organ I choose ?what disease I choose??? Any comment. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From SDrew <@t> uwhealth.org Wed Sep 26 13:58:54 2007 From: SDrew <@t> uwhealth.org (Drew Sally A.) Date: Wed Sep 26 13:58:59 2007 Subject: [Histonet] CD138 and Mycobacterium bovis In-Reply-To: Message-ID: If it's on human tissue, consider trying Serotec's antibody. We currently are using their concentrate on our Ventana instruments and are happy with it. Sally Ann Drew, MT(ASCP) IHC/ISH Laboratory University of Wisconsin Hosp. & Clinics Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ms. K?tia Cristina Catunda Sent: Wednesday, September 26, 2007 12:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD138 and Mycobacterium bovis Hi there, Does anybody has a clone recomendation for the antibody CD138?? We used to use clone MI15 from Dako but were informed that it was discontinued! And vendors for Mycobacterium bovis? antibody? Dako is not working well! Sincerely, Ms. K?tia Catunda Produ??o +55 12 3203-0612 (direto) +55 12 3203-0633 (PABX) www.cipax.com.br katia.catunda@cipax.com.br _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMcArdle <@t> ebsciences.com Wed Sep 26 13:59:45 2007 From: PMcArdle <@t> ebsciences.com (Phil McArdle) Date: Wed Sep 26 13:59:53 2007 Subject: [Histonet] Embedding polyurethane In-Reply-To: References: Message-ID: <46FAAC21.7020607@ebsciences.com> Hello: I'd have reservations about hexane - evil stuff. But as for IPA, assuming it's not India Pale Ale :-), that's a common shorthand for isopropyl alcohol (IUPAC 2-propanol), not to be confused with rubbing alcohol that's been watered down. I've had no experience with either hexane or IPA as an acetone replacement in this context, though. Good luck! Phil McArdle -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Mobile: 860.597.6796 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com I skate to where the puck is going to be, not to where it's been. - Wayne Gretsky You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it, and it was probably my mistake anyway. So please do the right thing and make this e-mail go away. Thank you. Boneslides@aol.com wrote: > Hello Histonetters!! > > Does anyone know if hexane can be used in place of acetone? I have a series > of orthopedic specimens that contain a polyurethane implant that the > researcher wants embedded in Spurr's and then rough cut and ground. I used my usual > protocol, but the acetone caused the polyurethane to swell. The company did > some research and was told that hexane could work and possibly something > called IPA, as these are both considered non-solvents for polyurethane. I am > not familiar with either of these substances. Any one have any insight?? > > Any help would be much appreciated! > > ~Diane > > > Diane M. Mahovlic, HT, MLT(ASCP) > Orthopedic Pathology & Biomaterials Laboratory > Department of Anatomic Pathology > The Cleveland Clinic Foundation > > 9500 Euclid Avenue- L30 > Cleveland, Ohio 44195 > 216-444-0166 > > > > ************************************** See what's new at http://www.aol.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Mobile: 860.597.6796 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com I skate to where the puck is going to be, not to where it's been. - Wayne Gretsky You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it, and it was probably my mistake anyway. So please do the right thing and make this e-mail go away. Thank you. From SDrew <@t> uwhealth.org Wed Sep 26 13:59:58 2007 From: SDrew <@t> uwhealth.org (Drew Sally A.) Date: Wed Sep 26 14:00:02 2007 Subject: [Histonet] CD138 and Mycobacterium bovis In-Reply-To: Message-ID: Sorry, I was only talking about the CD138... Sally Ann Drew, MT(ASCP) IHC/ISH Laboratory University of Wisconsin Hosp. & Clinics Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ms. K?tia Cristina Catunda Sent: Wednesday, September 26, 2007 12:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD138 and Mycobacterium bovis Hi there, Does anybody has a clone recomendation for the antibody CD138?? We used to use clone MI15 from Dako but were informed that it was discontinued! And vendors for Mycobacterium bovis? antibody? Dako is not working well! Sincerely, Ms. K?tia Catunda Produ??o +55 12 3203-0612 (direto) +55 12 3203-0633 (PABX) www.cipax.com.br katia.catunda@cipax.com.br _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Sep 26 14:30:56 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 26 14:31:00 2007 Subject: [Histonet] Hydrogen Peroxide Question In-Reply-To: Message-ID: <863462.36138.qm@web61222.mail.yahoo.com> 3% for 2-3 minutes Ren? J. "Ford, Judi" wrote: Hi Everyone, I wanted to post this for one of my co-workers. What percentage H2O2 is used to quench endogenous peroxidase in frozen section CD marker staining? how long? Thanks, Judi Ford Palo Alto, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Don't let your dream ride pass you by. Make it a reality with Yahoo! Autos. From carl.hobbs <@t> kcl.ac.uk Wed Sep 26 14:49:39 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Wed Sep 26 14:50:10 2007 Subject: [Histonet] Re: Histonet Digest, Vol 46, Issue 47 Message-ID: <001601c80076$621ba920$4101a8c0@carlba65530bda> ----- Original Message ----- From: To: Sent: Wednesday, September 26, 2007 4:23 PM Subject: Histonet Digest, Vol 46, Issue 47 > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: CPT code for finger nails using potassium hydroxide > (Rene J Buesa) > 2. RE: {SPAM?} [Histonet] Potassium Ferrocyanide Question > (Douglas D Deltour) > 3. Re: Potassium Ferrocyanide Question (Bryan Llewellyn) > 4. Laser Capture Microdissection Web Seminar (Chu, Shirley) > 5. Jacquelyn Grewe/Staff/OhioHealth is out of the office . > (JGREWE@OhioHealth.com) > 6. Re: CPT code for finger nails using potassium hydroxide > (Joe Nocito) > 7. RE: Potassium Ferrocyanide Question (Della Speranza, Vinnie) > 8. RE: Potassium Ferrocyanide Question (Tony Henwood) > 9. Position opening (Ernestine Middleton) > 10. How to choose a topic of a master degree thesis? (K M) > 11. wax on the rug (godsgalnow@aol.com) > 12. RE: CPT code for finger nails using potassium hydroxide > (Bernice Frederick) > 13. Re: How to choose a topic of a master degree thesis? > (Rene J Buesa) > 14. RE: {SPAM?} [Histonet] wax on the rug (Douglas D Deltour) > 15. Re: wax on the rug (Emily Sours) > 16. RE: wax on the rug (Weems, Joyce) > 17. Cytology Quality Improvement standards (godsgalnow@aol.com) > 18. RE: How to choose a topic of a master degree thesis? > (Kemlo Rogerson) > 19. FW: [Histonet] wax on the rug (Ian Montgomery) > 20. Re: Cytology Quality Improvement standards (Rene J Buesa) > 21. Re: wax on the rug (Greg Dobbin) > 22. Rugs and safety--PLEASE READ (Cheryl R. Kerry) > 23. RE: wax on the rug (CHRISTIE GOWAN) > 24. RE: wax on the rug (Patricia Adams) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 25 Sep 2007 10:00:39 -0700 (PDT) > From: Rene J Buesa > Subject: RE: [Histonet] CPT code for finger nails using potassium > hydroxide > To: "Weems, Joyce" , Vacca Jessica > , Histonet@lists.utsouthwestern.edu > Message-ID: <360416.16716.qm@web61216.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > There is no CPT code for KOH on nails and probably the cause is: > 1- not averybody soften nails before processing them; > 2- some HTs just "soften" nails while sectioning them, or > 3- is a procedure that takes only 30 min. with 10% KOH. > Regardless, it is another step and I think there should be a CPT code for > it. > Ren? J. > > > > > > > > > --------------------------------- > Shape Yahoo! in your own image. Join our Network Research Panel today! > > ------------------------------ > > Message: 2 > Date: Tue, 25 Sep 2007 13:03:22 -0500 > From: "Douglas D Deltour" > Subject: RE: {SPAM?} [Histonet] Potassium Ferrocyanide Question > To: "'Greg Dobbin'" , > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Formula weight is commonly abbreviated FW. The formula weight of a > substance > is the sum of the atomic weights of each atom in the chemical formula. > When the chemical formula equals the molecular formula, then the formula > weight is also called the molecular weight. The abbreviation commonly used > for molecular weight is MW. > > > > Douglas D. Deltour HT(ASCP) > Histology Manager > Professional Pathology Services, PC > One Science Court > Suite 200 > Columbia, SC 29203 > Office (803)252-1913 > Fax (803)254-3262 > Doug@ppspath.com > ***************************************************** > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of the individual or entity to > which it is addressed and may contain information that is privileged, > confidential and exempt from disclosure under applicable law. If the > reader > of this message is not the intended recipient, you are hereby notified > that > any dissemination, distribution, or copying of this communication is > strictly prohibited by law. If you have received this communication in > error, please notify me immediately. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Greg > Dobbin > Sent: Tuesday, September 25, 2007 11:33 AM > To: Histonet@lists.utsouthwestern.edu > Subject: {SPAM?} [Histonet] Potassium Ferrocyanide Question > > Hi Folks, > Chemistry is not my forte for sure, so a little help from the chemistry > gurus would be greatly appreciated. > > I purchased potassium ferrocyanide trihydrate for use in our Perl's > stain. The bottle that is almost empty lists the contents as > K4Fe(CN)6-3H2O with a molecular wt of 422.39. > > The new bottle (different supplier) uses the same name for the chemical > ("potassium ferrocyanide trihydrate") but the formula is listed as > FeK4N6-3H2O and the F.W. is 356.7. > > Can someone remind me what the "F" in F.W. stands for? And did I buy > the right chemical? Both are ACS grade chemicals. > > Thank you. > Greg > > Greg Dobbin, R.T. > Chief Technologist, Histology Lab > Dept. of Laboratory Medicine, > Queen Elizabeth Hospital, > P.O. Box 6600 > Charlottetown, PE C1A 8T5 > Phone: (902) 894-2337 > Fax: (902) 894-2385 > > > Statement of Confidentiality > This message (including attachments) may contain confidential or > privileged > information intended for a specific individual or organization. If you > have > received this communication in error, please notify the sender > immediately. > If you are not the intended recipient, you are not authorized to use, > disclose, distribute, copy, print or rely on this email, and should > promptly > delete this email from your entire computer system. > > D?claration de confidentialit? > Le pr?sent message (y compris les annexes) peut contenir des > renseignements > confidentiels ayant pour objet une personne ou un organisme particulier. > Si > vous avez re?u la pr?sente communication par erreur, veuillez en informer > l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous > n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer > ce > courriel ou encore de vous en servir, et vous devriez l'effacer > compl?tement > de votre syst?me informatique. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > ------------------------------ > > Message: 3 > Date: Tue, 25 Sep 2007 10:54:19 -0700 > From: Bryan Llewellyn > Subject: Re: [Histonet] Potassium Ferrocyanide Question > To: Histonet , Greg Dobbin > > Message-ID: <002501c7ff9d$18fed250$05024246@yourlk4rlmsu> > Content-Type: text/plain; format=flowed; charset=iso-8859-1; > reply-type=original > > Potassium ferrocyanide is usually given the formula you first noted. > However, my Merck Index also gives the formula C6FeK4N6 with a MW of > 368.34 > (you may have a typo and left out the C6). The differences in MW may be > due > to including or not including the 3H2O. > > Just do a test Perls' stain on an iron control section. If they are the > same then don't worry about it. > > Bryan Llewellyn > > > > ----- Original Message ----- > From: "Greg Dobbin" > To: > Sent: Tuesday, September 25, 2007 9:32 AM > Subject: [Histonet] Potassium Ferrocyanide Question > > >> Hi Folks, >> Chemistry is not my forte for sure, so a little help from the chemistry >> gurus would be greatly appreciated. >> >> I purchased potassium ferrocyanide trihydrate for use in our Perl's >> stain. The bottle that is almost empty lists the contents as >> K4Fe(CN)6-3H2O with a molecular wt of 422.39. >> >> The new bottle (different supplier) uses the same name for the chemical >> ("potassium ferrocyanide trihydrate") but the formula is listed as >> FeK4N6-3H2O and the F.W. is 356.7. >> >> Can someone remind me what the "F" in F.W. stands for? And did I buy >> the right chemical? Both are ACS grade chemicals. >> >> Thank you. >> Greg >> >> Greg Dobbin, R.T. >> Chief Technologist, Histology Lab >> Dept. of Laboratory Medicine, >> Queen Elizabeth Hospital, >> P.O. Box 6600 >> Charlottetown, PE C1A 8T5 >> Phone: (902) 894-2337 >> Fax: (902) 894-2385 >> >> >> Statement of Confidentiality >> This message (including attachments) may contain confidential or >> privileged information intended for a specific individual or >> organization. >> If you have received this communication in error, please notify the >> sender >> immediately. If you are not the intended recipient, you are not >> authorized to use, disclose, distribute, copy, print or rely on this >> email, and should promptly delete this email from your entire computer >> system. >> >> D?claration de confidentialit? >> Le pr?sent message (y compris les annexes) peut contenir des >> renseignements confidentiels ayant pour objet une personne ou un >> organisme >> particulier. Si vous avez re?u la pr?sente communication par erreur, >> veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le >> destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, >> distribuer, copier ou imprimer ce courriel ou encore de vous en servir, >> et >> vous devriez l'effacer compl?tement de votre syst?me informatique. >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 4 > Date: Tue, 25 Sep 2007 14:25:35 -0400 > From: "Chu, Shirley" > Subject: [Histonet] Laser Capture Microdissection Web Seminar > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > An Arcturus LCM webinar will be presented on Tues, Oct 16th by Anna > Pecherskaya of the Fox Chase Cancer Center. The title of her talk is: > "Practical Applications of Laser Capture Microdissection". There is no > cost to attend this webinar. An abstract of this presentation and > registration information can be found on the following website: > http://www.moleculardevices.com/pages/webinar_arcturus.html > > > > Shirley Chu > > Applications Scientist, Arcturus LCM Products > > Molecular Devices > > (now a part of MDS Analytical Technologies) > > 510-675-6260 | www.moleculardevices.com > > > > > ------------------------------ > > Message: 5 > Date: Tue, 25 Sep 2007 16:01:09 -0400 > From: JGREWE@OhioHealth.com > Subject: [Histonet] Jacquelyn Grewe/Staff/OhioHealth is out of the > office . > To: histonet@lists.utsouthwestern.edu > Message-ID: > > > Content-Type: text/plain; charset=US-ASCII > > > I will be out of the office starting 09/24/2007 and will not return until > 09/30/2007. > > I will respond to your message when I return. Thanks, Jackie > > > > > ------------------------------ > > Message: 6 > Date: Tue, 25 Sep 2007 16:18:57 -0500 > From: "Joe Nocito" > Subject: Re: [Histonet] CPT code for finger nails using potassium > hydroxide > To: "Rene J Buesa" , "Weems, Joyce" > , "Vacca Jessica" , > > Message-ID: <000c01c7ffb9$b03da0d0$0202a8c0@yourxhtr8hvc4p> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > I have to agree with Rene. There is a CPT for decals, there should be one > for nails. And this is coming from Joe the Toe. > ----- Original Message ----- > From: "Rene J Buesa" > To: "Weems, Joyce" ; "Vacca Jessica" > ; > Sent: Tuesday, September 25, 2007 12:00 PM > Subject: RE: [Histonet] CPT code for finger nails using potassium > hydroxide > > >> There is no CPT code for KOH on nails and probably the cause is: >> 1- not averybody soften nails before processing them; >> 2- some HTs just "soften" nails while sectioning them, or >> 3- is a procedure that takes only 30 min. with 10% KOH. >> Regardless, it is another step and I think there should be a CPT code >> for >> it. >> Ren? J. >> >> >> >> >> >> >> >> >> --------------------------------- >> Shape Yahoo! in your own image. Join our Network Research Panel today! >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 7 > Date: Tue, 25 Sep 2007 17:38:05 -0400 > From: "Della Speranza, Vinnie" > Subject: RE: [Histonet] Potassium Ferrocyanide Question > To: "Greg Dobbin" , > > Message-ID: > <7F6B678A32B0564196138E6B3101996ABF208B@EVS1.clinlan.local> > Content-Type: text/plain; charset="us-ascii" > > Greg, In comparing the two formulas you typed I don't see the cyanide > (CN) in the second formula so I'm sort of confused. Is that a typo on > your part ? > > to answer your question more specifically, if the chemicals were the > same the formula weight (FW) should be the same, and clearly your new > chemical is quite different from the first. > > Vinnie Della Speranza > Manager for Anatomic Pathology Services > 165 Ashley Avenue Suite 309 > Charleston, South Carolina 29425 > Tel: (843) 792-6353 > Fax: (843) 792-8974 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Greg > Dobbin > Sent: Tuesday, September 25, 2007 12:33 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Potassium Ferrocyanide Question > > Hi Folks, > Chemistry is not my forte for sure, so a little help from the chemistry > gurus would be greatly appreciated. > > I purchased potassium ferrocyanide trihydrate for use in our Perl's > stain. The bottle that is almost empty lists the contents as > K4Fe(CN)6-3H2O with a molecular wt of 422.39. > > The new bottle (different supplier) uses the same name for the chemical > ("potassium ferrocyanide trihydrate") but the formula is listed as > FeK4N6-3H2O and the F.W. is 356.7. > > Can someone remind me what the "F" in F.W. stands for? And did I buy > the right chemical? Both are ACS grade chemicals. > > Thank you. > Greg > > Greg Dobbin, R.T. > Chief Technologist, Histology Lab > Dept. of Laboratory Medicine, > Queen Elizabeth Hospital, > P.O. Box 6600 > Charlottetown, PE C1A 8T5 > Phone: (902) 894-2337 > Fax: (902) 894-2385 > > > Statement of Confidentiality > This message (including attachments) may contain confidential or > privileged information intended for a specific individual or > organization. If you have received this communication in error, please > notify the sender immediately. If you are not the intended recipient, > you are not authorized to use, disclose, distribute, copy, print or rely > on this email, and should promptly delete this email from your entire > computer system. > > D?claration de confidentialit? > Le pr?sent message (y compris les annexes) peut contenir des > renseignements confidentiels ayant pour objet une personne ou un > organisme particulier. Si vous avez re?u la pr?sente communication par > erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes > pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, > divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous > en servir, et vous devriez l'effacer compl?tement de votre syst?me > informatique. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 8 > Date: Wed, 26 Sep 2007 08:43:30 +1000 > From: "Tony Henwood" > Subject: RE: [Histonet] Potassium Ferrocyanide Question > To: "Greg Dobbin" , > > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > FW means Formula weight. It is usually the same as the molecular weight. > BUT > According to the formulas you have recorded in your email, the second is > missing a few carbons ie: > K4Fe(CN)6-3H2O with a molecular wt of 422.39 > FeK4N6-3H2O and the F.W. is 356.7. > Is this correct? > > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) > Laboratory Manager & Senior Scientist > The Children's Hospital at Westmead, > Locked Bag 4001, Westmead, 2145, AUSTRALIA. > Tel: 612 9845 3306 > Fax: 612 9845 3318 > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Greg > Dobbin > Sent: Wednesday, 26 September 2007 2:33 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Potassium Ferrocyanide Question > > > Hi Folks, > Chemistry is not my forte for sure, so a little help from the chemistry > gurus would be greatly appreciated. > > I purchased potassium ferrocyanide trihydrate for use in our Perl's > stain. The bottle that is almost empty lists the contents as > K4Fe(CN)6-3H2O with a molecular wt of 422.39. > > The new bottle (different supplier) uses the same name for the chemical > ("potassium ferrocyanide trihydrate") but the formula is listed as > FeK4N6-3H2O and the F.W. is 356.7. > > Can someone remind me what the "F" in F.W. stands for? And did I buy the > right chemical? Both are ACS grade chemicals. > > Thank you. > Greg > > Greg Dobbin, R.T. > Chief Technologist, Histology Lab > Dept. of Laboratory Medicine, > Queen Elizabeth Hospital, > P.O. Box 6600 > Charlottetown, PE C1A 8T5 > Phone: (902) 894-2337 > Fax: (902) 894-2385 > > > Statement of Confidentiality > This message (including attachments) may contain confidential or > privileged information intended for a specific individual or > organization. If you have received this communication in error, please > notify the sender immediately. If you are not the intended recipient, > you are not authorized to use, disclose, distribute, copy, print or rely > on this email, and should promptly delete this email from your entire > computer system. > > D?claration de confidentialit? > Le pr?sent message (y compris les annexes) peut contenir des > renseignements confidentiels ayant pour objet une personne ou un > organisme particulier. Si vous avez re?u la pr?sente communication par > erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes > pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, > divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous > en servir, et vous devriez l'effacer compl?tement de votre syst?me > informatique. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************* > This email and any files transmitted with it are confidential and intended > solely for the use of the individual or entity to whom they are addressed. > If you are not the intended recipient, please delete it and notify the > sender. > > Views expressed in this message and any attachments are those of the > individual sender, and are not necessarily the views of The Children's > Hospital at Westmead > > This note also confirms that this email message has been > virus scanned and although no computer viruses were detected, The > Childrens Hospital at Westmead accepts no liability for any consequential > damage resulting from email containing computer viruses. > ********************************************************************** > > > > > ------------------------------ > > Message: 9 > Date: Tue, 25 Sep 2007 20:46:21 -0400 (EDT) > From: Ernestine Middleton > Subject: [Histonet] Position opening > To: histonet@pathology.swmed.edu > Message-ID: <877501.47437.qm@web51511.mail.re2.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hi: > Montefiore Medical Center, Bronx, NY is seeking Mohs technologist. Must > have NYS license. Will accept a parttime personnel until and full time > person can be found. > Please e-mail resume to emiddlet@montefiore.org. > > We are still looking for evening Histology supervirsor. > > Ernestine Middleton > Montefiore Medical Center > Bronx, NY > 718-920-4157 > 718-547-1920 fax > emiddlet@montefiore.org > > > --------------------------------- > Ask a question on any topic and get answers from real people. Go to Yahoo! > Answers. > > ------------------------------ > > Message: 10 > Date: Wed, 26 Sep 2007 13:20:12 +0200 > From: "K M" > Subject: [Histonet] How to choose a topic of a master degree thesis? > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Glad to be here.This is my very post here.Iam preparing to do a master > degree in anatomical pathology and I am little confused > how can I choose a topic to work on my thesis? My supervisor said to me go > to GOOGLE but google made me more confused! > By the way I am in Egypt and interest in immunohistochemistry studies .but > which organ I choose ?what disease I choose??? > Any comment. > > > ------------------------------ > > Message: 11 > Date: Wed, 26 Sep 2007 08:27:48 -0400 > From: godsgalnow@aol.com > Subject: [Histonet] wax on the rug > To: histonet@lists.utsouthwestern.edu > Message-ID: <8C9CE4FC8B1ABE2-2CC-579@FWM-D01.sysops.aol.com> > Content-Type: text/plain; charset="us-ascii" > > Hello 'Netters, > > > Anyone out there know how to get paraffin out of the rug?? You know, from > tracking it into and down the hallway on your feet? > > I do know that they have the sticky tape that you can put on the floor of > the lab before the carpet begins to take the wax off your shoes, but the > owner of the lab thinks it looks horrible and doesn't like it. > > Thanks in advance.... > > Roxanne > ________________________________________________________________________ > Email and AIM finally together. You've gotta check out free AOL Mail! - > http://mail.aol.com > > > ------------------------------ > > Message: 12 > Date: Wed, 26 Sep 2007 07:32:21 -0500 > From: "Bernice Frederick" > Subject: RE: [Histonet] CPT code for finger nails using potassium > hydroxide > To: "'Joe Nocito'" , "'Rene J Buesa'" > , "'Weems, Joyce'" , "'Vacca > Jessica'" , > > Message-ID: <004801c80039$4c5601e0$d00f7ca5@lurie.northwestern.edu> > Content-Type: text/plain; charset="iso-8859-1" > > All- I would think decal is decal no matter the means to do so. We charge > decal even it's a surface decal. We use Nair or Neet to soften nails. > Bernice > > Bernice Frederick HTL (ASCP) > Northwestern University > Pathology Core Facility > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito > Sent: Tuesday, September 25, 2007 4:19 PM > To: Rene J Buesa; Weems, Joyce; Vacca Jessica; > Histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] CPT code for finger nails using potassium > hydroxide > > I have to agree with Rene. There is a CPT for decals, there should be one > for nails. And this is coming from Joe the Toe. > ----- Original Message ----- > From: "Rene J Buesa" > To: "Weems, Joyce" ; "Vacca Jessica" > ; > Sent: Tuesday, September 25, 2007 12:00 PM > Subject: RE: [Histonet] CPT code for finger nails using potassium > hydroxide > > >> There is no CPT code for KOH on nails and probably the cause is: >> 1- not averybody soften nails before processing them; >> 2- some HTs just "soften" nails while sectioning them, or >> 3- is a procedure that takes only 30 min. with 10% KOH. >> Regardless, it is another step and I think there should be a CPT code >> for > >> it. >> Ren? J. >> >> >> >> >> >> >> >> >> --------------------------------- >> Shape Yahoo! in your own image. Join our Network Research Panel today! >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 13 > Date: Wed, 26 Sep 2007 05:35:33 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] How to choose a topic of a master degree > thesis? > To: K M , histonet@lists.utsouthwestern.edu > Message-ID: <260472.4026.qm@web61213.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > KM > Why don't you try a comparison of melanin precursors identifiers? > > There are many antibodies you can use, and you can make comparisons about > their "early detection" efficacy, for which you can use different types of > lesions, from nevus to pre-melanomas to melanomas correlating the reaction > intensities with the antibodies dilution rates.Under separate cover I am > sending you the abstract of a paper on the subject and the results of some > experiments on the topic I did in 2002 > You should consult with your pathologist to select the specimens for your > study. > Ren? J. > > K M wrote: > Glad to be here.This is my very post here.Iam preparing to do a master > degree in anatomical pathology and I am little confused > how can I choose a topic to work on my thesis? My supervisor said to me go > to GOOGLE but google made me more confused! > By the way I am in Egypt and interest in immunohistochemistry studies .but > which organ I choose ?what disease I choose??? > Any comment. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Got a little couch potato? > Check out fun summer activities for kids. > > ------------------------------ > > Message: 14 > Date: Wed, 26 Sep 2007 08:43:47 -0500 > From: "Douglas D Deltour" > Subject: RE: {SPAM?} [Histonet] wax on the rug > To: , > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Roxanne, > > I am not sure if you want to go to the trouble but I have used this > method... > 1. Obtain an iron and a brown grocery bag. > 2. Place one layer of the paper bag over the spot with wax. > 3. With your iron on medium high, and iron over the paper bag over the > place with wax. > 4. Move the bag around if necessary. The wax will warm up and stick to > the grocery bag. > 5. Be diligent until all wax is gone. You may need more than one paper > bag, depending on the size of the hardened wax. > 6. Use a lower setting for delicate materials on the iron. > > I have also used paper towels in place of the grocery bag. > > Douglas D. Deltour HT(ASCP) > Histology Manager > Professional Pathology Services, PC > One Science Court > Suite 200 > Columbia, SC 29203 > Office (803)252-1913 > Fax (803)254-3262 > Doug@ppspath.com > ***************************************************** > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of the individual or entity to > which it is addressed and may contain information that is privileged, > confidential and exempt from disclosure under applicable law. If the > reader > of this message is not the intended recipient, you are hereby notified > that > any dissemination, distribution, or copying of this communication is > strictly prohibited by law. If you have received this communication in > error, please notify me immediately. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > godsgalnow@aol.com > Sent: Wednesday, September 26, 2007 7:28 AM > To: histonet@lists.utsouthwestern.edu > Subject: {SPAM?} [Histonet] wax on the rug > > Hello 'Netters, > > > Anyone out there know how to get paraffin out of the rug?? You know, from > tracking it into and down the hallway on your feet? > > I do know that they have the sticky tape that you can put on the floor of > the lab before the carpet begins to take the wax off your shoes, but the > owner of the lab thinks it looks horrible and doesn't like it. > > Thanks in advance.... > > Roxanne > ________________________________________________________________________ > Email and AIM finally together. You've gotta check out free AOL Mail! - > http://mail.aol.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > ------------------------------ > > Message: 15 > Date: Wed, 26 Sep 2007 08:47:00 -0400 > From: "Emily Sours" > Subject: Re: [Histonet] wax on the rug > To: "godsgalnow@aol.com" , > histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > The way I do it at home is put a paper bag over the wax and iron the > carpet > through the paper bag. Set the iron on low! > The wax should be absorbed by the paper bag and your carpet is saved. > This is probably not a good idea for large areas, unless you have a lot of > time. > > Emily > -- > as soon as you tolerate something, it becomes bearable, and before long it > will become common > --Israel Zangwill > > > ------------------------------ > > Message: 16 > Date: Wed, 26 Sep 2007 08:54:06 -0400 > From: "Weems, Joyce" > Subject: RE: [Histonet] wax on the rug > To: , > Message-ID: > <1CD6831EB9B26D45B0A3EAA79F7EBD32048F3CD6@sjhaexc02.sjha.org> > Content-Type: text/plain; charset="utf-8" > > Depending on how much is there, freeze it with freon and pop it up. If it > is ground in, you might try ironing (not too hot!) over a brown grocery > bag. Tacky mats for operating rooms are helpful in keeping the amount of > paraffin down. > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 404-851-7376 - Phone > 404-851-7831 - Fax > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of > godsgalnow@aol.com > Sent: Wednesday, September 26, 2007 8:28 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] wax on the rug > > > Hello 'Netters, > > > Anyone out there know how to get paraffin out of the rug?? You know, from > tracking it into and down the hallway on your feet? > > I do know that they have the sticky tape that you can put on the floor of > the lab before the carpet begins to take the wax off your shoes, but the > owner of the lab thinks it looks horrible and doesn't like it. > > Thanks in advance.... > > Roxanne > ________________________________________________________________________ > Email and AIM finally together. You've gotta check out free AOL Mail! - > http://mail.aol.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice ** The information contained in this message may be > privileged and is confidential information intended for the use of the > addressee listed above. If you are neither the intended recipient nor the > employee or agent responsible for delivering this message to the intended > recipient, you are hereby notified that any disclosure, copying, > distribution or the taking of any action in reliance on the contents of > this information is strictly prohibited. If you have received this > communication in error, please notify us immediately by replying to the > message and deleting it from your computer. Thank you. Saint Joseph's > Health System, Inc. > > ------------------------------ > > Message: 17 > Date: Wed, 26 Sep 2007 09:26:04 -0400 > From: godsgalnow@aol.com > Subject: [Histonet] Cytology Quality Improvement standards > To: histonet@pathology.swmed.edu > Message-ID: <8C9CE57EC453070-2CC-8A6@FWM-D01.sysops.aol.com> > Content-Type: text/plain; charset="us-ascii" > > > I am the Lab Supervisor for a private lab that does urine cytology, as > well as the bladder biopsies and prostate biopsies.? We do FISH as well.? > I am not a cytotech and we are trying to develop some QA/QI standards and > guidelines.? Currently we do not have a QA program in place for our > routine cytology, other than the Histo/Cyto Correlation because all > screened slides are signed out by a Pathologist so we get 100 QA on them > anyway. > > But, when I go back to do the Histo/Cyto Correlation, there are sometimes > discrepancies.? For instance, the cytology will be negative, the FISH will > be negative, but the bladder biopsies will show TCC.? So, in order to > continue to stop doing the Correlation of these findings in a vacuum, we > need some kind of standards as to what % discrepant diagnosis is > acceptable.? How many false positives, false negatives, etc..can be > expected. > > Does anyone have any insight on this and can shed some light on this for > me?? > > Roxanne > > ________________________________________________________________________ > Email and AIM finally together. You've gotta check out free AOL Mail! - > http://mail.aol.com > > > ------------------------------ > > Message: 18 > Date: Wed, 26 Sep 2007 14:32:27 +0100 > From: "Kemlo Rogerson" > Subject: RE: [Histonet] How to choose a topic of a master degree > thesis? > To: "K M" , > Message-ID: > <86ADE4EB583CE64799A9924684A0FBBF0222EDA5@wahtntex2.waht.swest.nhs.uk> > Content-Type: text/plain; charset="us-ascii" > > Secret is to do something on something you enjoy. Don't try and do an > MSc on something you think is a tad boring as it will get very, very > boring!! I assume you have a Supervisor? Your Uni would have one PLUS > you need one at your place of work; what does your supervisor say? Next > try and find someone, like a Medic, that has a specific interest but no > time to pursue it. If you try and come up with something that your > organisation has no interest in then you will struggle. > > If you have a Research Department ask them, if you know of anyone > carrying out research then ask them. Unfortunately immunocytochemistry > interests me not at all so I can't help and if someone from the Forum > suggests something then you will be in an academically sterile > atmosphere, struggling by yourself. Why not carry out some > immuncytochemistry on Mummies? Might get a grant and involved in a > research programme, but I've no idea what you could look for in mummies > or how you would get the tissue. > > Kemlo Rogerson > Pathology Manager > DD 01934 647057 or extension 3311 > Mob 07749 754194; Pager 07659 597107; > > > Loneliness > Is > An island > In > The middle > Of > A sea of people --R.R. (age 11) > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. > Please inform me that this message has gone astray before deleting it. > Thank you for your co-operation > > > > > > ------------------------------ > > Message: 19 > Date: Wed, 26 Sep 2007 14:46:38 +0100 > From: "Ian Montgomery" > Subject: FW: [Histonet] wax on the rug > To: > Message-ID: <003501c80043$a99bd4b0$6424d182@IBLS.GLA.AC.UK> > Content-Type: text/plain; charset="us-ascii" > > Roxanne, > Brown wrapping paper and a hot iron, then repeat until the wax > has been removed. Or, claim the insurance and buy a new one. > Ian. > > Dr. Ian Montgomery, > Histotechnology, > I.B.L.S. Support Unit, > Thomson Building, > University of Glasgow, > G12 8QQ. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > godsgalnow@aol.com > Sent: 26 September 2007 13:28 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] wax on the rug > > Hello 'Netters, > > > Anyone out there know how to get paraffin out of the rug?? You know, from > tracking it into and down the hallway on your feet? > > I do know that they have the sticky tape that you can put on the floor of > the lab before the carpet begins to take the wax off your shoes, but the > owner of the lab thinks it looks horrible and doesn't like it. > > Thanks in advance.... > > Roxanne > ________________________________________________________________________ > Email and AIM finally together. You've gotta check out free AOL Mail! - > http://mail.aol.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 20 > Date: Wed, 26 Sep 2007 06:46:42 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Cytology Quality Improvement standards > To: godsgalnow@aol.com, histonet@pathology.swmed.edu > Message-ID: <747836.20548.qm@web61211.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > There is an interdepartmental CAP program, similar to the one for IHC, > that you can subscribe to. The screeners and pathologists participate and > the slides they send for interpretation are graded and this is one of the > best and strongest QA existing programs. > Also your pathologists should be the ones to answer the questions you > have, specially those referring to ASCUS and false positives or negatives > that should tend to be "0" > Ren? J. > > godsgalnow@aol.com wrote: > > I am the Lab Supervisor for a private lab that does urine cytology, as > well as the bladder biopsies and prostate biopsies.? We do FISH as well.? > I am not a cytotech and we are trying to develop some QA/QI standards and > guidelines.? Currently we do not have a QA program in place for our > routine cytology, other than the Histo/Cyto Correlation because all > screened slides are signed out by a Pathologist so we get 100 QA on them > anyway. > > But, when I go back to do the Histo/Cyto Correlation, there are sometimes > discrepancies.? For instance, the cytology will be negative, the FISH will > be negative, but the bladder biopsies will show TCC.? So, in order to > continue to stop doing the Correlation of these findings in a vacuum, we > need some kind of standards as to what % discrepant diagnosis is > acceptable.? How many false positives, false negatives, etc..can be > expected. > > Does anyone have any insight on this and can shed some light on this for > me?? > > Roxanne > > ________________________________________________________________________ > Email and AIM finally together. You've gotta check out free AOL Mail! - > http://mail.aol.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Boardwalk for $500? In 2007? Ha! > Play Monopoly Here and Now (it's updated for today's economy) at Yahoo! > Games. > > ------------------------------ > > Message: 21 > Date: Wed, 26 Sep 2007 11:12:59 -0300 > From: "Greg Dobbin" > Subject: Re: [Histonet] wax on the rug > To: > Message-ID: > Content-Type: text/plain; charset=US-ASCII > > Am I the only one who sees the humour in this subject line?!? :-) > Greg > > Greg Dobbin, R.T. > Chief Technologist, Histology Lab > Dept. of Laboratory Medicine, > Queen Elizabeth Hospital, > P.O. Box 6600 > Charlottetown, PE C1A 8T5 > Phone: (902) 894-2337 > Fax: (902) 894-2385 > > >>>> 9/26/2007 9:27 AM >>> > Hello 'Netters, > > > Anyone out there know how to get paraffin out of the rug?? You know, > from tracking it into and down the hallway on your feet? > > I do know that they have the sticky tape that you can put on the floor > of the lab before the carpet begins to take the wax off your shoes, but > the owner of the lab thinks it looks horrible and doesn't like it. > > Thanks in advance.... > > Roxanne > ________________________________________________________________________ > Email and AIM finally together. You've gotta check out free AOL Mail! - > http://mail.aol.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Statement of Confidentiality > This message (including attachments) may contain confidential or > privileged information intended for a specific individual or organization. > If you have received this communication in error, please notify the sender > immediately. If you are not the intended recipient, you are not > authorized to use, disclose, distribute, copy, print or rely on this > email, and should promptly delete this email from your entire computer > system. > > D?claration de confidentialit? > Le pr?sent message (y compris les annexes) peut contenir des > renseignements confidentiels ayant pour objet une personne ou un organisme > particulier. Si vous avez re?u la pr?sente communication par erreur, > veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le > destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, > distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et > vous devriez l'effacer compl?tement de votre syst?me informatique. > > > > ------------------------------ > > Message: 22 > Date: Wed, 26 Sep 2007 10:01:55 -0500 > From: "Cheryl R. Kerry" > Subject: [Histonet] Rugs and safety--PLEASE READ > To: > Message-ID: <004001c8004e$2e4df850$6401a8c0@CHERYLSLAPTOP> > Content-Type: text/plain; charset="us-ascii" > > When you utilize rugs and such, LISTEN to your staff!!! > > If they complain of tripping or having problems with the rugs > buckling--LISTEN and DO SOMETHING!!! > > As you all know I run a temp agency and I've been a working tech for a > long > time. We work with labs that use rugs--and I've had them in labs I've > managed. We taped the buckles to the floor or required the rental company > to purchase industrial quality rugs to keep things safe. We changed the > wheel on the chairs to reduce the lifting by rolling across the edges. > All > created a safer lab at very little cost. > > In one of our client labs the techs have been tripping and complaining > about > the hazard when at work. Unfortunately the hazard never filtered back to > our office until it was too late. One of our techs tripped last night and > ended up in an ambulance to the ER. She could've broken a hip the way she > fell--it was horrible. This was not an unknown hazard for the lab or the > techs--which creates a liability issue on top of the injury. > > If anyone brings a safety issue to you--please listen!! They aren't just > complaining to complain. No one wants to be off of work for a preventable > injury. Rugs create a safer, cleaner floor, but only when properly > maintained. > > Thanks for listening--it was a really long night. She's going to be okay > but > it's going to be a sore couple of weeks. I'm sure I'm going to get flamed > but if one less person is injured because of this--fine--bring it. > > Cheryl > > Cheryl R. Kerry, HT(ASCP) > Full Staff Inc. > Staffing the AP Lab, one great tech at a time. > 281.852.9457 office > 281.883.7704 cell > 800.756.3309 fax and alternate phone > > > > > ------------------------------ > > Message: 23 > Date: Wed, 26 Sep 2007 15:03:27 +0000 > From: "CHRISTIE GOWAN" > Subject: RE: [Histonet] wax on the rug > To: godsgalnow@aol.com, histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; format=flowed > > I would check with a carpet cleaning company that specializes in > commercial > and industrial jobs. Perhaps they have a solvent based cleaner that > removes > the buildup of tracked paraffin. In the meantime I would have the hospital > put down carpet mats that can be switched out and cleaned periodically. My > 2 > cents. > Christie Gowan > > >>From: godsgalnow@aol.com >>To: histonet@lists.utsouthwestern.edu >>Subject: [Histonet] wax on the rug >>Date: Wed, 26 Sep 2007 08:27:48 -0400 >> >>Hello 'Netters, >> >> >>Anyone out there know how to get paraffin out of the rug?? You know, from >>tracking it into and down the hallway on your feet? >> >>I do know that they have the sticky tape that you can put on the floor of >>the lab before the carpet begins to take the wax off your shoes, but the >>owner of the lab thinks it looks horrible and doesn't like it. >> >>Thanks in advance.... >> >>Roxanne >>________________________________________________________________________ >>Email and AIM finally together. You've gotta check out free AOL Mail! - >>http://mail.aol.com >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 24 > Date: Wed, 26 Sep 2007 08:16:07 -0700 (PDT) > From: Patricia Adams > Subject: RE: [Histonet] wax on the rug > To: CHRISTIE GOWAN , HistoNet > > Message-ID: <354044.95362.qm@web52512.mail.re2.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > A hospital that I worked for had their cleaning > group use a steam cleaner to remove the paraffin > from the carpets. We had two sets one to put > down while the other was sent to be cleaned. > Worked great. > Patricia > --- CHRISTIE GOWAN wrote: > >> I would check with a carpet cleaning company >> that specializes in commercial >> and industrial jobs. Perhaps they have a >> solvent based cleaner that removes >> the buildup of tracked paraffin. In the >> meantime I would have the hospital >> put down carpet mats that can be switched out >> and cleaned periodically. My 2 >> cents. >> Christie Gowan >> >> >> >From: godsgalnow@aol.com >> >To: histonet@lists.utsouthwestern.edu >> >Subject: [Histonet] wax on the rug >> >Date: Wed, 26 Sep 2007 08:27:48 -0400 >> > >> >Hello 'Netters, >> > >> > >> >Anyone out there know how to get paraffin out >> of the rug?? You know, from >> >tracking it into and down the hallway on your >> feet? >> > >> >I do know that they have the sticky tape that >> you can put on the floor of >> >the lab before the carpet begins to take the >> wax off your shoes, but the >> >owner of the lab thinks it looks horrible and >> doesn't like it. >> > >> >Thanks in advance.... >> > >> >Roxanne >> >>________________________________________________________________________ >> >Email and AIM finally together. You've gotta >> check out free AOL Mail! - >> >http://mail.aol.com >> >>_______________________________________________ >> >Histonet mailing list >> >Histonet@lists.utsouthwestern.edu >> >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > Patricia Adams > ----- > Fight back spam! Download the Blue Frog. > http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D > > > > ____________________________________________________________________________________ > Pinpoint customers who are looking for what you sell. > http://searchmarketing.yahoo.com/ > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 46, Issue 47 > **************************************** From settembr <@t> umdnj.edu Wed Sep 26 14:56:05 2007 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Wed Sep 26 14:56:58 2007 Subject: [Histonet] Hydrogen Peroxide Question Message-ID: I use 3% for 5 minutes. 3 minutes sounds good for a better turn around time for the patient. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Rene J Buesa 09/26/07 3:30 PM >>> 3% for 2-3 minutes Ren? J. "Ford, Judi" wrote: Hi Everyone, I wanted to post this for one of my co-workers. What percentage H2O2 is used to quench endogenous peroxidase in frozen section CD marker staining? how long? Thanks, Judi Ford Palo Alto, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Don't let your dream ride pass you by. Make it a reality with Yahoo! Autos. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ploykasek <@t> phenopath.com Wed Sep 26 14:59:51 2007 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Wed Sep 26 14:59:57 2007 Subject: [Histonet] Hydrogen Peroxide Question In-Reply-To: Message-ID: We use H2O2 from the grocery store, works great. For FFPE, use it on depar. slides for 5 min. However, I have found that on frozen tissue, some CD markers are adversely affected by H2O2. On frozen tissue, we use an azide block instead. I could send you the details or check histonet archives. Hope this helps. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > Hi Everyone, > > I wanted to post this for one of my co-workers. > > What percentage H2O2 is used to quench endogenous peroxidase in frozen > section CD marker staining? how long? > > Thanks, > > Judi Ford > > Palo Alto, CA > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From ploykasek <@t> phenopath.com Wed Sep 26 15:03:43 2007 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Wed Sep 26 15:03:52 2007 Subject: [Histonet] CD138 and Mycobacterium bovis In-Reply-To: Message-ID: We are using clone B-B4. It can be purchased from BioCare Medical. We were purchasing it from a different company, but it has been discontinued. The discontinuation of some antibodies has been the bane of my existence this year. As some companies have been bought by larger companies, this has become more of a problem (at least that's my perception). Patient care seems to take a back seat to profit margin. (please don't flame me!) Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > Hi there, > > Does anybody has a clone recomendation for the antibody CD138?? > We used to use clone MI15 from Dako but were informed that it was > discontinued! > > And vendors for Mycobacterium bovis? antibody? Dako is not working > well! > > > Sincerely, > > > Ms. K?tia Catunda > Produ??o > +55 12 3203-0612 (direto) > +55 12 3203-0633 (PABX) > www.cipax.com.br > katia.catunda@cipax.com.br > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From doug <@t> ppspath.com Wed Sep 26 16:37:25 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Wed Sep 26 15:37:50 2007 Subject: [Histonet] Ohh the pain (replacing formalin) Message-ID: I have found a suitable replacement for xylene but that is the easy part. I now have been directed to start looking for a formalin substitute. I am not aware of anyone that actually uses the stuff (probably for good reason). Does anyone know of or have any experience with these alternative (inferior) products? Does anyone have any experience with the validation (headache) required to actually replace formalin? I can hardly wait. Thanks. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. From hej01 <@t> health.state.ny.us Wed Sep 26 15:41:44 2007 From: hej01 <@t> health.state.ny.us (Helen E Johnson) Date: Wed Sep 26 15:41:51 2007 Subject: [Histonet] CD 138 Message-ID: Hi Histonetters, Does anyone have an IHC protocol for CD 138 [Rat anti-mouse] [Unconjugated] for FFPE mouse spleen? Helen Johnson (hej01@health.state.ny.us) IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. From MElliott <@t> mrl.ubc.ca Wed Sep 26 15:49:44 2007 From: MElliott <@t> mrl.ubc.ca (Mark Elliott) Date: Wed Sep 26 15:50:36 2007 Subject: [Histonet] C5b-9 antibody for pig tissue In-Reply-To: <0B16AF64.781@mail.mrl.ubc.ca> References: <0B16AF64.781@mail.mrl.ubc.ca> Message-ID: <46FA6376.11C6.00D6.0@mrl.ubc.ca> Does anyone know of an antibody to C5b-9 which will work on formalin-fixed paraffin embedded pig tissue Thanks Mark ***CONFIDENTIALITY NOTICE*** This electronic message is intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From katia.catunda <@t> cipax.com.br Wed Sep 26 16:03:39 2007 From: katia.catunda <@t> cipax.com.br (=?iso-8859-1?Q?Ms._K=E1tia_Cristina_Catunda?=) Date: Wed Sep 26 16:03:25 2007 Subject: [Histonet] GLUT1 In-Reply-To: Message-ID: Hi again! Does anybody knows any vendors for GLUT1?? Another antibody that has been discontinued!! Sincerely, Ms. K?tia Catunda Produ??o +55 12 3203-0612 (direto) +55 12 3203-0633 (PABX) www.cipax.com.br katia.catunda@cipax.com.br From Eric.Hoy <@t> UTSouthwestern.edu Wed Sep 26 16:14:01 2007 From: Eric.Hoy <@t> UTSouthwestern.edu (Eric Hoy) Date: Wed Sep 26 16:14:09 2007 Subject: [Histonet] Re: Histonet Digest, Vol 46, Issue 49 In-Reply-To: <200709262042.l8QKgMXC005739@flpi183.prodigy.net> Message-ID: Previously on Histonet: Mark Tarango said: > From: "Tarango, Mark" > Subject: RE: [Histonet] How to choose a topic of a master degree > thesis? > > Choose something that you can match up with some other assays. If you > are going to do IHC pick a gene and use an antibody against the > protein...and then come up with a good story about what it all means. > Do some FISH, PCR and other stuff to support the IHC. Doing IHC alone > would most likely make for a really boring paper. In reply to: > Glad to be here.This is my very post here.Iam preparing to do a master > degree in anatomical pathology and I am little confused > how can I choose a topic to work on my thesis? My supervisor said to me > go > to GOOGLE but google made me more confused! > By the way I am in Egypt and interest in immunohistochemistry studies > .but > which organ I choose ?what disease I choose??? > Any comment. My comment: I agree with Mark Tarango. The IHC should just be one technique that you use in pursuing a more general question. When I did my dissertation, I used IHC in conjunction with other histopathologic findings, immunological tests, and microbial cell culture to look at the pathology of Chlamydial pneumonitis in newborn mice. The IHC photos were a big hit with everyone on my examining committee, but they were only a small part of the overall data. My second opinion is that Google is a terrible place to look for a topic for research. I agree with those who have said to talk to mentors at your university. That way, when a problem arises (and I assure you that one will), you will have someone local to consult. Best wishes for your thesis research! Eric Hoy (Ph.D. In Pathology, University of Illinois College of Medicine, 1987) =================================================== Eric S. Hoy, Ph.D., SI(ASCP) Clinical Associate Professor Department of Medical Laboratory Sciences The University of Texas Southwestern Medical Center Dallas, Texas Email: Eric.Hoy@UTSouthwestern.edu =================================================== From Ronald.Houston <@t> nationwidechildrens.org Wed Sep 26 16:13:31 2007 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Wed Sep 26 16:14:18 2007 Subject: [Histonet] GLUT1 In-Reply-To: Message-ID: <979FF5962E234F45B06CF0DB7C1AABB21246606C@chi2k3ms01.columbuschildrens.net> LabVision has a polyclonal antibody to Glut-1 which is an IVD Cat#: RB-9052 www.thermo.com/labvision Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 Ronald.Houston@NationwideChildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ms. K?tia Cristina Catunda Sent: Wednesday, September 26, 2007 5:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GLUT1 Hi again! Does anybody knows any vendors for GLUT1?? Another antibody that has been discontinued!! Sincerely, Ms. K?tia Catunda Produ??o +55 12 3203-0612 (direto) +55 12 3203-0633 (PABX) www.cipax.com.br katia.catunda@cipax.com.br _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dellav <@t> musc.edu Wed Sep 26 16:16:25 2007 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Wed Sep 26 16:15:12 2007 Subject: [Histonet] mycobacterium tuberculosis and fixation In-Reply-To: References: Message-ID: <7F6B678A32B0564196138E6B3101996AC48059@EVS1.clinlan.local> Liz, Funny you should ask this question. I authored an article in the Dec 2002 HistoLogic entitled "Does Formaldehyde Kill Mycobacterium tuberculosis?" The article was to some extent a review of the scant body of literature on this topic. I wrote it because we had a pathologist on staff who insisted that TB could survive processing and I didn't believe it. You should be able to find the article at Sakura's website but if you cannot I may have a pdf of that issue that I can send you or I can fax it if you send me your fax number. The anecdotal reports of TB surviving fixation were typically in autopsy or embalmed remains. As you can imagine, the first question to consider is whether the tissue was thoroughly and completely fixed. With autopsy tissues, organs are often breadloafed and immersed in fixative but if great care is not exercised, it is not uncommon to see red, apparently unfixed areas when cutting down organs or viscera if breadloafing was not adequately done. Embalming is even more tenuous because a number of factors can affect whether embalming fluid successfully reaches all tissues of the body. I don't believe anyone can definitively tell you that TB cannot survive processing but I am doubtful that there is any reason to be concerned. I'm aware of no regulatory requirements for the clinical lab pertaining to the preparation of paraffin sections of TB positive cases. The presumption is that exposure to formalin and alcohol will neutralize the organism during the processing cycle. If you are looking for a guarantee however I'm doubtful anyone can offer you one. Conversely we have stringent requirements for the handling of fresh tissues and frozen sections of unfixed samples when TB is expected. Histonet is a fairly large community. It will be interesting to see if anyone can report knowledge of a colleague contracting TB specifically from exposure to fixed, paraffin processed tissue. I think if there was any substantial risk we would all be aware of it but I could be wrong. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Wednesday, September 26, 2007 12:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] mycobacterium tuberculosis and fixation I'm posting this question since I need to research the viability of mycobaterium tuberculosis in fixed tissue samples. We quite freqently handle paraformaldehye fixed samples from guinea pigs and mice that have been that infected with MTB, after fixation they are stored in 70% alchol for weeks before I get them. I have culture data that shows that these samples that I am receiving have no viable TB present. I'm wondering if there is anyone out there that handles fixed TB samples differently or are they considered non-infectious. There has been one published study that did find viable bacteria in autopsy samples that had been stored in 10% NBF so there is conflicting data. Our policy on these type of samples is that we only accept fixed tissue samples, we do not work with any unfixed tissue samples. We do make available for individuals that are grossing in the samples a 3M N95 particulate respirator, wearing the respirator is not manditory and we have run this procedure past our local OSHA person and they are fine with it. The one concern I have is that we use a shop vac to suck up the paraffin debris from the microtome and is this causing aerosolization. Any comments are appreciated and thanks in advance. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 HYPERLINK "mailto:liz@premierlab.com"liz@premierlab.com HYPERLINK "http://www.premierlab.com/"www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.5.488 / Virus Database: 269.13.30/1030 - Release Date: 9/25/2007 8:02 AM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dellav <@t> musc.edu Wed Sep 26 16:21:47 2007 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Wed Sep 26 16:20:29 2007 Subject: [Histonet] Ohh the pain (replacing formalin) In-Reply-To: <5teknr$5nd3rb@ironp1.musc.edu> References: <5teknr$5nd3rb@ironp1.musc.edu> Message-ID: <7F6B678A32B0564196138E6B3101996AC4805C@EVS1.clinlan.local> Hi Doug, It probably wouldn't be fair to say formalin substitutes are "inferior" but they will for the most part be different. If you are looking for something that behaves exactly like formalin and gives the same morphologic and immunostaining results, you may want to keep a bottle of ibuprofen near by as you begin your search. If such a thing existed we'd all be using it. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Wednesday, September 26, 2007 5:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ohh the pain (replacing formalin) I have found a suitable replacement for xylene but that is the easy part. I now have been directed to start looking for a formalin substitute. I am not aware of anyone that actually uses the stuff (probably for good reason). Does anyone know of or have any experience with these alternative (inferior) products? Does anyone have any experience with the validation (headache) required to actually replace formalin? I can hardly wait. Thanks. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Daivik_Shah <@t> rush.edu Wed Sep 26 16:44:52 2007 From: Daivik_Shah <@t> rush.edu (Daivik_Shah@rush.edu) Date: Wed Sep 26 16:45:05 2007 Subject: [Histonet] Re: Hydrogen Peroxide quenching In-Reply-To: <200709262118.l8QLIrUj021786@symav6.cc1.rpslmc.edu> Message-ID: Hello friends, I have question regarding Hydrogen peroxide quenching. Is this treatment applied in Immunohistostain after deparafinization or before application of secondary antibody? Is that affect any way to stain? Thanks. Daivik _____________________________ Daivik Shah, Histotechnologist, M.S. Biotechnology RADC Laboratory, Cohn Building 436 Rush University Medical Center Tel. 312-563-4891 This message and any attachments contain information for the exclusive use of the individual or entity to whom it is addressed and may contain information that is privileged, confidential and/or exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, or the employee or agent, you are hereby notified that any distribution or copying of this communication is strictly prohibited. If you received this message in error, please phone me immediately at 312-563-4891. From rjbuesa <@t> yahoo.com Wed Sep 26 16:49:25 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 26 16:49:29 2007 Subject: [Histonet] Re: Hydrogen Peroxide quenching In-Reply-To: Message-ID: <414935.83772.qm@web61222.mail.yahoo.com> You use it immediately after the HIER, before starting the IHC protocol. Ren? J. Daivik_Shah@rush.edu wrote: Hello friends, I have question regarding Hydrogen peroxide quenching. Is this treatment applied in Immunohistostain after deparafinization or before application of secondary antibody? Is that affect any way to stain? Thanks. Daivik _____________________________ Daivik Shah, Histotechnologist, M.S. Biotechnology RADC Laboratory, Cohn Building 436 Rush University Medical Center Tel. 312-563-4891 This message and any attachments contain information for the exclusive use of the individual or entity to whom it is addressed and may contain information that is privileged, confidential and/or exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, or the employee or agent, you are hereby notified that any distribution or copying of this communication is strictly prohibited. If you received this message in error, please phone me immediately at 312-563-4891. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Got a little couch potato? Check out fun summer activities for kids. From judi.ford <@t> roche.com Wed Sep 26 17:03:44 2007 From: judi.ford <@t> roche.com (Ford, Judi) Date: Wed Sep 26 17:03:58 2007 Subject: [Histonet] Re: Hydrogen Peroxide quenching In-Reply-To: <414935.83772.qm@web61222.mail.yahoo.com> References: <414935.83772.qm@web61222.mail.yahoo.com> Message-ID: Okay, so I'm confused. Some labs do the hydrogen peroxide step before HIER and others afterwards, why? Also, are there differences using hydrogen peroxide diluted in water/methanol/or PBS? Some bring paraffin slides down to absolute alcohol then do the hydrogen peroxide step where others wait until bringing the slides to water before doing the step. Is there some rule of thumb, or do you just figure out which procedure works for that specific antibody in your lab? I'm still pretty new to this, so sorry if this seems like a dumb question. Judi F. Palo Alto, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, September 26, 2007 2:49 PM To: Daivik_Shah@rush.edu; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Hydrogen Peroxide quenching You use it immediately after the HIER, before starting the IHC protocol. Ren? J. Daivik_Shah@rush.edu wrote: Hello friends, I have question regarding Hydrogen peroxide quenching. Is this treatment applied in Immunohistostain after deparafinization or before application of secondary antibody? Is that affect any way to stain? Thanks. Daivik _____________________________ Daivik Shah, Histotechnologist, M.S. Biotechnology RADC Laboratory, Cohn Building 436 Rush University Medical Center Tel. 312-563-4891 This message and any attachments contain information for the exclusive use of the individual or entity to whom it is addressed and may contain information that is privileged, confidential and/or exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, or the employee or agent, you are hereby notified that any distribution or copying of this communication is strictly prohibited. If you received this message in error, please phone me immediately at 312-563-4891. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Got a little couch potato? Check out fun summer activities for kids. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Wed Sep 26 17:15:19 2007 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed Sep 26 17:15:29 2007 Subject: [Histonet] Ohh the pain Message-ID: <582736990709261515w4bd1f849g86fac5a87d5f64fe@mail.gmail.com> Douglas, I've really gotta write this up someday and save it so I can just cut & paste it every time someone winds me up on this rant. I'll keep it gentle or try to at least! :-) The premise of my argument is that no lab is an island. If you use a fixative that is different from everyone else then all subsequent steps, from cutting to staining are different. Different fixatives alter the tissue in different ways. So, if you fix the same piece of tissue as another person in another lab, and you each use a different fixative, formalin or not, subsequent testing may be drastically different. So you need to ask a few questions in this process. Is all the tissue you process going to be fixed in the new fixative, if not, how will you be able to tell them apart? Are you going to be sending ANY tissue out to other institutions for testing? If so how do they know how you fixed it and do they have the capabilities to handle tissue fixed in various ways? Are you going to recieve tissue from another institution, and if so how do you test it if all your testing is based upon the alternative fixation technique? Do you have any archive tissue, if so how does the results of the new tests correlate to the same tests on archived tissue. What do you use as control tissue for both tests. How does the fixative affect any future clinical studies? Granted there will be some tests that changing the fixative will have no effect on. Alternative fixative vendors would show you they can get many tests to work fine. When hearing this just think about the above questions. I've heard many good answers to one or two of them. I've never heard of anyone able to answer ALL of them! It may be a great idea to dump formalin as a fixative in favor of another. I think the only way to do it properly is to unanamously choose ONE fixative and use it as the standard in most if not all labs in the country if not the world. Sounds nice right? Standardization of testing begins here. Now ask what's in the fixative ... OOH that's a proprietary secret! So much for standardization! Until these pompous money grubbing fools that sell this snake oil loosen up and start making the formulations 'open source' formalin will remain the industry standard. Just my $0.02 (again), Amos Brooks PS: I hope I haven't offended anybody with the ferocity of any of the above statements. I like sales people, I like most of these companies. I don't like policies that put the dollar sign before the patient and the science used to save them. Date: Wed, 26 Sep 2007 16:37:25 -0500 From: "Douglas D Deltour" Subject: [Histonet] Ohh the pain (replacing formalin) To: Message-ID: Content-Type: text/plain; charset="us-ascii" I have found a suitable replacement for xylene but that is the easy part. I now have been directed to start looking for a formalin substitute. I am not aware of anyone that actually uses the stuff (probably for good reason). Does anyone know of or have any experience with these alternative (inferior) products? Does anyone have any experience with the validation (headache) required to actually replace formalin? I can hardly wait. Thanks. From jnocito <@t> satx.rr.com Wed Sep 26 18:21:30 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Sep 26 18:21:36 2007 Subject: [Histonet] Re: Hydrogen Peroxide quenching References: <414935.83772.qm@web61222.mail.yahoo.com> Message-ID: <003301c80093$f93016d0$0302a8c0@yourxhtr8hvc4p> Judi, Judi Judi (sorry, couldn't help it) always, always, always. Did I mention always? Always do what is right for the lab you are in. I have tried H2O2 blocking before HIER, after HIER, after the secondary antibody and everywhere in between. As long as you block before the DAB you should be fine. Most of the time you can get away with what someone else is using. Sometimes it'll be a trial and error. Whatever works best in your situation Not every lab is the same and not every tech is the same. Your tissue processing might be different for the next lab, the temp & humidity in your lab may be different from the next lab. I know this may seem like a cop out, but it's true. When I was transferred from D.C. to San Antonio to set up an immuno lab, I had my former techs FedEx me some reagents to my new lab. Now, I had the same reagents, same procedures and same tech. I still had some adjustments to do in the new lab because I was getting a high, non-specific background staining. The environment was different as was the tissue processing. This is why my philosophy is there is no such thing as a pre-diluted antibody. Keep in mind that the companies perform THEIR testing in THEIR lab, not yours. I always try a short titer to see if I can get a 1:2 or a 1:4 dilution. Look it at it this way, if I can get a 1:4 dilution out of a pre-dilute, and the vial has 6 mls, I just purchased 24 mls for the cost of 6 mls (is that correct? Math was never my subject). Did ramble on again? Hey, not that this is a big thing, but I join the work force again on Monday. I'm keeping the place secret so I don't get called in by CEOs any more. JTT ----- Original Message ----- From: "Ford, Judi" To: "Rene J Buesa" ; ; Sent: Wednesday, September 26, 2007 5:03 PM Subject: RE: [Histonet] Re: Hydrogen Peroxide quenching Okay, so I'm confused. Some labs do the hydrogen peroxide step before HIER and others afterwards, why? Also, are there differences using hydrogen peroxide diluted in water/methanol/or PBS? Some bring paraffin slides down to absolute alcohol then do the hydrogen peroxide step where others wait until bringing the slides to water before doing the step. Is there some rule of thumb, or do you just figure out which procedure works for that specific antibody in your lab? I'm still pretty new to this, so sorry if this seems like a dumb question. Judi F. Palo Alto, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, September 26, 2007 2:49 PM To: Daivik_Shah@rush.edu; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Hydrogen Peroxide quenching You use it immediately after the HIER, before starting the IHC protocol. Ren? J. Daivik_Shah@rush.edu wrote: Hello friends, I have question regarding Hydrogen peroxide quenching. Is this treatment applied in Immunohistostain after deparafinization or before application of secondary antibody? Is that affect any way to stain? Thanks. Daivik _____________________________ Daivik Shah, Histotechnologist, M.S. Biotechnology RADC Laboratory, Cohn Building 436 Rush University Medical Center Tel. 312-563-4891 This message and any attachments contain information for the exclusive use of the individual or entity to whom it is addressed and may contain information that is privileged, confidential and/or exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, or the employee or agent, you are hereby notified that any distribution or copying of this communication is strictly prohibited. If you received this message in error, please phone me immediately at 312-563-4891. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Got a little couch potato? Check out fun summer activities for kids. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dianapaczesny <@t> gmail.com Wed Sep 26 19:48:59 2007 From: dianapaczesny <@t> gmail.com (Diana Paczesny) Date: Wed Sep 26 19:49:07 2007 Subject: [Histonet] slides of undecal rat molar teeth Message-ID: Hello I am doing a project on pulp capping in rat molar teeth and would like slides of undecalcified sections of the teeth, however, my lab does not have the facilities for this. From my understanding, they would have to be embedded in resin and then cut, and it seems like making 10um sections is very difficult because of the undecal tooth. Any advice on a lab that would do this for me? Thanks Dr Diana Paczesny From talulahgosh <@t> gmail.com Wed Sep 26 23:41:47 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Wed Sep 26 23:41:52 2007 Subject: [Histonet] Re: Maleic acid vs. Tris in in situ buffer In-Reply-To: <46FA8EC7.1020105@uci.edu> References: <46FA8EC7.1020105@uci.edu> Message-ID: I've never heard of using maleic acid for DIG in situ's. This could be because we have followed Schaeren-Wiemers and Gerfin-Moser (1993) protocol, which uses Tris-HCl and makes no mention of maleic acid. In the papers I've read using DIG in situ's (with chick embryonic tissue, mind you), there has been no mention of maleic acid. Where have you seen this in a protocol? I suggest "if it ain't broke, don't fix it". When we've tried to make our in situ's better by using someone else's protocol, we've *always* gone back to our original protocol because it's much better. After ten years of using it, we know it kicks ass. Well, at least I do--my PI has doubts, but she comes around on day three. :) By the way, is this for whole mount or section in situ's? And if for sections, for paraffin or frozen, at what thickness? I'm interested in knowing in case this helps for paraffin in situ's, since we had beautiful results and then nothing (though the embryos were fixed in toluene, which didn't work, instead of xylene, which worked). Paraffin in situ's would rock my world. yes, paraffin in situ's rock my world. because i love my job. Emily -- Yog-Sothoth knows the gate. Yog-Sothoth is the gate. Yog-Sothoth is the key and guardian of the gate. Past, present, future, all are one in Yog-Sothoth. He knows where the Old Ones broke through of old, and where They shall break through again. From chengkaz <@t> uci.edu Thu Sep 27 01:02:50 2007 From: chengkaz <@t> uci.edu (Chengkang ZHANG) Date: Thu Sep 27 01:02:50 2007 Subject: [Histonet] Re: Maleic acid vs. Tris in in situ buffer In-Reply-To: References: <46FA8EC7.1020105@uci.edu> Message-ID: <46FB478A.7080408@uci.edu> Emily, thanks for the input. I've also followed the protocol of Schaeren-Weimers and Gerfin-Moser's as well and it worked pretty good for me (14 um frozen section of adult and embryonic brains). However, I came across with this Maleic acid protocol when I read a recent paper from Oscar Marin's lab (J. Neurosci, 2007, 27, 9682-9695). Then I went back and checked the data sheet coming with the anti-DIG-AP antibody (Roche), I found that they suggest using Maleic acid based buffer for "membrane application" and Tris-based buffer for "other application". And a round of Google search of Maleic acid buffer turned up tons of protocols using maleic acid based buffer for all kinds of in situ, either whole mount, frozen or paraffin sections. So I am curious why maleic acid would be preferred in other protocols, as it has much less buffering capacity than Tris in the pH range of 7-8. Google search revealed one explanation that maleic acid buffer can be treated with DEPC-H2O, while Tris buffer can't. The explanation makes sense, but for posthybridization steps in situ, DEPC-treated buffers are not necessary. So is there another reason for the preference of maleic acid? You are right by saying "if it ain't broke, don't fix it", and I am going to stick with my old protocol. But, still, a little curiosity hangs over here. ================================== Chengkang Zhang Ph.D. Room 357, MedSurge II Department of Pharmacology University of California, Irvine Irvine, CA 92697-4625 Email: chengkaz@uci.edu Tel: (949)-824-1902 (lab) ================================== Emily Sours wrote: > I've never heard of using maleic acid for DIG in situ's. This could > be because we have followed Schaeren-Wiemers and Gerfin-Moser (1993) > protocol, which uses Tris-HCl and makes no mention of maleic acid. > In the papers I've read using DIG in situ's (with chick embryonic > tissue, mind you), there has been no mention of maleic acid. Where > have you seen this in a protocol? > I suggest "if it ain't broke, don't fix it". When we've tried to make > our in situ's better by using someone else's protocol, we've *always* > gone back to our original protocol because it's much better. After > ten years of using it, we know it kicks ass. Well, at least I do--my > PI has doubts, but she comes around on day three. :) > By the way, is this for whole mount or section in situ's? And if for > sections, for paraffin or frozen, at what thickness? I'm interested in > knowing in case this helps for paraffin in situ's, since we had > beautiful results and then nothing (though the embryos were fixed in > toluene, which didn't work, instead of xylene, which worked). > Paraffin in situ's would rock my world. > > yes, paraffin in situ's rock my world. because i love my job. > Emily > -- > Yog-Sothoth knows the gate. > Yog-Sothoth is the gate. > Yog-Sothoth is the key and guardian of the gate. Past, present, > future, all are one in Yog-Sothoth. > He knows where the Old Ones broke through of old, and where They shall > break through again. From talulahgosh <@t> gmail.com Thu Sep 27 01:33:22 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu Sep 27 01:33:27 2007 Subject: [Histonet] Re: Maleic acid vs. Tris in in situ buffer In-Reply-To: <46FB478A.7080408@uci.edu> References: <46FA8EC7.1020105@uci.edu> <46FB478A.7080408@uci.edu> Message-ID: That is true--Tris solutions cannot be treated with DEPC before inactivation (ie before autoclaving), but we have always made our solutions with DEPC water that has been autoclaved (and thus inactivated for DEPC) and with chemicals that are separate from other chemicals that are used from non-RNase free protocols. We have not found that treating our solutions for RNase afterwards (ie, adding DEPC to the solution) has made a difference--though we are very careful about what is RNase free and what is not (if you would like a very short info session on this, let me know!) I'll have to check your reference out--that's interesting that someone tried maleic acid recently, and who knows, it could help with certain applications. Thanks for the interesting inquiry; if you try it out, let me know how it goes. Emily ps. In my own opinion, as a tech and NOT an expert, treating solutions with DEPC after they have been made (as opposed to making a solution with DEPC water and RNase free chemicals) doesn't make a difference at all. In fact, I consider it a hindrance, because you don't know if your chemical is affected by DEPC, or if it will evaporate in the autoclave as you try to inactivate the DEPC) We always make our in situ solutions with DEPC treated water and not the opposite, treating the final solution with DEPC. -- Yog-Sothoth knows the gate. Yog-Sothoth is the gate. Yog-Sothoth is the key and guardian of the gate. Past, present, future, all are one in Yog-Sothoth. He knows where the Old Ones broke through of old, and where They shall break through again. From ree3 <@t> leicester.ac.uk Thu Sep 27 03:26:54 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Thu Sep 27 03:27:12 2007 Subject: [Histonet] Re: Hydrogen Peroxide quenching In-Reply-To: References: <414935.83772.qm@web61222.mail.yahoo.com> Message-ID: It is not a dumb question at all, every lab does things their way for a variety of reasons, the main one being of course "because we have always done it this way, and it works for us", with the real reasons why things are done in a particular way are lost in the mists of times long long ago, when the world was young and global warming had not been thought about. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ford, Judi Sent: 26 September 2007 23:04 To: Rene J Buesa; Daivik_Shah@rush.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Hydrogen Peroxide quenching Okay, so I'm confused. Some labs do the hydrogen peroxide step before HIER and others afterwards, why? Also, are there differences using hydrogen peroxide diluted in water/methanol/or PBS? Some bring paraffin slides down to absolute alcohol then do the hydrogen peroxide step where others wait until bringing the slides to water before doing the step. Is there some rule of thumb, or do you just figure out which procedure works for that specific antibody in your lab? I'm still pretty new to this, so sorry if this seems like a dumb question. Judi F. Palo Alto, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, September 26, 2007 2:49 PM To: Daivik_Shah@rush.edu; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Hydrogen Peroxide quenching You use it immediately after the HIER, before starting the IHC protocol. Ren? J. Daivik_Shah@rush.edu wrote: Hello friends, I have question regarding Hydrogen peroxide quenching. Is this treatment applied in Immunohistostain after deparafinization or before application of secondary antibody? Is that affect any way to stain? Thanks. Daivik _____________________________ Daivik Shah, Histotechnologist, M.S. Biotechnology RADC Laboratory, Cohn Building 436 Rush University Medical Center Tel. 312-563-4891 This message and any attachments contain information for the exclusive use of the individual or entity to whom it is addressed and may contain information that is privileged, confidential and/or exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, or the employee or agent, you are hereby notified that any distribution or copying of this communication is strictly prohibited. If you received this message in error, please phone me immediately at 312-563-4891. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Got a little couch potato? Check out fun summer activities for kids. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From louise.renton <@t> gmail.com Thu Sep 27 06:19:37 2007 From: louise.renton <@t> gmail.com (louise renton) Date: Thu Sep 27 06:19:46 2007 Subject: [Histonet] smad 6&7 controls Message-ID: Hi there, is ANYONE out there doing immuno on the above (or knows something about these proteins) who could suggest which tissues I could use for controls? Much appreciated -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From doug <@t> ppspath.com Thu Sep 27 07:50:59 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Thu Sep 27 06:52:17 2007 Subject: [Histonet] Ohh the pain In-Reply-To: <582736990709261515w4bd1f849g86fac5a87d5f64fe@mail.gmail.com> Message-ID: You are preaching to the choir Amos. Believe me, this is not my idea. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: Amos Brooks [mailto:amosbrooks@gmail.com] Sent: Wednesday, September 26, 2007 5:15 PM To: doug@ppspath.com; histonet@lists.utsouthwestern.edu Subject: [Histonet] Ohh the pain Douglas, I've really gotta write this up someday and save it so I can just cut & paste it every time someone winds me up on this rant. I'll keep it gentle or try to at least! :-) The premise of my argument is that no lab is an island. If you use a fixative that is different from everyone else then all subsequent steps, from cutting to staining are different. Different fixatives alter the tissue in different ways. So, if you fix the same piece of tissue as another person in another lab, and you each use a different fixative, formalin or not, subsequent testing may be drastically different. So you need to ask a few questions in this process. Is all the tissue you process going to be fixed in the new fixative, if not, how will you be able to tell them apart? Are you going to be sending ANY tissue out to other institutions for testing? If so how do they know how you fixed it and do they have the capabilities to handle tissue fixed in various ways? Are you going to recieve tissue from another institution, and if so how do you test it if all your testing is based upon the alternative fixation technique? Do you have any archive tissue, if so how does the results of the new tests correlate to the same tests on archived tissue. What do you use as control tissue for both tests. How does the fixative affect any future clinical studies? Granted there will be some tests that changing the fixative will have no effect on. Alternative fixative vendors would show you they can get many tests to work fine. When hearing this just think about the above questions. I've heard many good answers to one or two of them. I've never heard of anyone able to answer ALL of them! It may be a great idea to dump formalin as a fixative in favor of another. I think the only way to do it properly is to unanamously choose ONE fixative and use it as the standard in most if not all labs in the country if not the world. Sounds nice right? Standardization of testing begins here. Now ask what's in the fixative ... OOH that's a proprietary secret! So much for standardization! Until these pompous money grubbing fools that sell this snake oil loosen up and start making the formulations 'open source' formalin will remain the industry standard. Just my $0.02 (again), Amos Brooks PS: I hope I haven't offended anybody with the ferocity of any of the above statements. I like sales people, I like most of these companies. I don't like policies that put the dollar sign before the patient and the science used to save them. Date: Wed, 26 Sep 2007 16:37:25 -0500 From: "Douglas D Deltour" Subject: [Histonet] Ohh the pain (replacing formalin) To: Message-ID: Content-Type: text/plain; charset="us-ascii" I have found a suitable replacement for xylene but that is the easy part. I now have been directed to start looking for a formalin substitute. I am not aware of anyone that actually uses the stuff (probably for good reason). Does anyone know of or have any experience with these alternative (inferior) products? Does anyone have any experience with the validation (headache) required to actually replace formalin? I can hardly wait. Thanks. From JWEEMS <@t> sjha.org Thu Sep 27 07:50:43 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Thu Sep 27 07:51:08 2007 Subject: [Histonet] Ohh the pain In-Reply-To: Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F3CFA@sjhaexc02.sjha.org> Remember that FDA requires formalin fixation for Her2/Neu testing, if this affects you at all.. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Douglas D Deltour Sent: Thursday, September 27, 2007 8:51 AM To: 'Amos Brooks'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Ohh the pain You are preaching to the choir Amos. Believe me, this is not my idea. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: Amos Brooks [mailto:amosbrooks@gmail.com] Sent: Wednesday, September 26, 2007 5:15 PM To: doug@ppspath.com; histonet@lists.utsouthwestern.edu Subject: [Histonet] Ohh the pain Douglas, I've really gotta write this up someday and save it so I can just cut & paste it every time someone winds me up on this rant. I'll keep it gentle or try to at least! :-) The premise of my argument is that no lab is an island. If you use a fixative that is different from everyone else then all subsequent steps, from cutting to staining are different. Different fixatives alter the tissue in different ways. So, if you fix the same piece of tissue as another person in another lab, and you each use a different fixative, formalin or not, subsequent testing may be drastically different. So you need to ask a few questions in this process. Is all the tissue you process going to be fixed in the new fixative, if not, how will you be able to tell them apart? Are you going to be sending ANY tissue out to other institutions for testing? If so how do they know how you fixed it and do they have the capabilities to handle tissue fixed in various ways? Are you going to recieve tissue from another institution, and if so how do you test it if all your testing is based upon the alternative fixation technique? Do you have any archive tissue, if so how does the results of the new tests correlate to the same tests on archived tissue. What do you use as control tissue for both tests. How does the fixative affect any future clinical studies? Granted there will be some tests that changing the fixative will have no effect on. Alternative fixative vendors would show you they can get many tests to work fine. When hearing this just think about the above questions. I've heard many good answers to one or two of them. I've never heard of anyone able to answer ALL of them! It may be a great idea to dump formalin as a fixative in favor of another. I think the only way to do it properly is to unanamously choose ONE fixative and use it as the standard in most if not all labs in the country if not the world. Sounds nice right? Standardization of testing begins here. Now ask what's in the fixative ... OOH that's a proprietary secret! So much for standardization! Until these pompous money grubbing fools that sell this snake oil loosen up and start making the formulations 'open source' formalin will remain the industry standard. Just my $0.02 (again), Amos Brooks PS: I hope I haven't offended anybody with the ferocity of any of the above statements. I like sales people, I like most of these companies. I don't like policies that put the dollar sign before the patient and the science used to save them. Date: Wed, 26 Sep 2007 16:37:25 -0500 From: "Douglas D Deltour" Subject: [Histonet] Ohh the pain (replacing formalin) To: Message-ID: Content-Type: text/plain; charset="us-ascii" I have found a suitable replacement for xylene but that is the easy part. I now have been directed to start looking for a formalin substitute. I am not aware of anyone that actually uses the stuff (probably for good reason). Does anyone know of or have any experience with these alternative (inferior) products? Does anyone have any experience with the validation (headache) required to actually replace formalin? I can hardly wait. Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From kimtournear <@t> yahoo.com Thu Sep 27 07:57:23 2007 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Thu Sep 27 07:57:30 2007 Subject: [Histonet] Dermatopathology question.... Message-ID: <968407.81746.qm@web50610.mail.re2.yahoo.com> Hi everyone, I work in a busy dermatology office. We process our own histology and do Mohs. We have 5 dermatologists and 3 P.A.'s. 1 of our dermatologists is a Board Certified Dermatopathologist. My question is: Since we have a Dermatopathologist in house, can a dermatologist, who is fluent in reading slides, (but not a certified as a dermatopathologist), read slides for another dermatologist (to help keep up)? Are there any legalities that I should be aware of? Any help would be greatly appreciated.....thanks in advance.... Kim Tournear, HT (ASCP), QIHC ( ASCP) Specialists in Dermatology Histology/Mohs Supervisor Tucson, AZ --------------------------------- Catch up on fall's hot new shows on Yahoo! TV. Watch previews, get listings, and more! From doug <@t> ppspath.com Thu Sep 27 09:12:31 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Thu Sep 27 08:12:55 2007 Subject: {SPAM?} RE: [Histonet] Ohh the pain In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F3CFA@sjhaexc02.sjha.org> Message-ID: Under the CAP/ASCO guidelines it reads "Any alteration of standard conditions, such as use of alternative fixatives, microwave fixation, or alternative processing methods, must be validated against standard methods of testing before a test routinely using these conditions is offered in a laboratory. Validation must consist of testing of the same samples with the alternative fixative buffered formalin using the same Her2 testing method to demonstrate concordance of the result". If there is more to it then please point me to the correct references. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Thursday, September 27, 2007 7:51 AM To: Douglas D Deltour; Amos Brooks; histonet@lists.utsouthwestern.edu Subject: {SPAM?} RE: [Histonet] Ohh the pain Remember that FDA requires formalin fixation for Her2/Neu testing, if this affects you at all.. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Douglas D Deltour Sent: Thursday, September 27, 2007 8:51 AM To: 'Amos Brooks'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Ohh the pain You are preaching to the choir Amos. Believe me, this is not my idea. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: Amos Brooks [mailto:amosbrooks@gmail.com] Sent: Wednesday, September 26, 2007 5:15 PM To: doug@ppspath.com; histonet@lists.utsouthwestern.edu Subject: [Histonet] Ohh the pain Douglas, I've really gotta write this up someday and save it so I can just cut & paste it every time someone winds me up on this rant. I'll keep it gentle or try to at least! :-) The premise of my argument is that no lab is an island. If you use a fixative that is different from everyone else then all subsequent steps, from cutting to staining are different. Different fixatives alter the tissue in different ways. So, if you fix the same piece of tissue as another person in another lab, and you each use a different fixative, formalin or not, subsequent testing may be drastically different. So you need to ask a few questions in this process. Is all the tissue you process going to be fixed in the new fixative, if not, how will you be able to tell them apart? Are you going to be sending ANY tissue out to other institutions for testing? If so how do they know how you fixed it and do they have the capabilities to handle tissue fixed in various ways? Are you going to recieve tissue from another institution, and if so how do you test it if all your testing is based upon the alternative fixation technique? Do you have any archive tissue, if so how does the results of the new tests correlate to the same tests on archived tissue. What do you use as control tissue for both tests. How does the fixative affect any future clinical studies? Granted there will be some tests that changing the fixative will have no effect on. Alternative fixative vendors would show you they can get many tests to work fine. When hearing this just think about the above questions. I've heard many good answers to one or two of them. I've never heard of anyone able to answer ALL of them! It may be a great idea to dump formalin as a fixative in favor of another. I think the only way to do it properly is to unanamously choose ONE fixative and use it as the standard in most if not all labs in the country if not the world. Sounds nice right? Standardization of testing begins here. Now ask what's in the fixative ... OOH that's a proprietary secret! So much for standardization! Until these pompous money grubbing fools that sell this snake oil loosen up and start making the formulations 'open source' formalin will remain the industry standard. Just my $0.02 (again), Amos Brooks PS: I hope I haven't offended anybody with the ferocity of any of the above statements. I like sales people, I like most of these companies. I don't like policies that put the dollar sign before the patient and the science used to save them. Date: Wed, 26 Sep 2007 16:37:25 -0500 From: "Douglas D Deltour" Subject: [Histonet] Ohh the pain (replacing formalin) To: Message-ID: Content-Type: text/plain; charset="us-ascii" I have found a suitable replacement for xylene but that is the easy part. I now have been directed to start looking for a formalin substitute. I am not aware of anyone that actually uses the stuff (probably for good reason). Does anyone know of or have any experience with these alternative (inferior) products? Does anyone have any experience with the validation (headache) required to actually replace formalin? I can hardly wait. Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From Daivik_Shah <@t> rush.edu Thu Sep 27 08:13:18 2007 From: Daivik_Shah <@t> rush.edu (Daivik_Shah@rush.edu) Date: Thu Sep 27 08:13:37 2007 Subject: [Histonet] Re: Hydrogen Peroxide quenching In-Reply-To: <003301c80093$f93016d0$0302a8c0@yourxhtr8hvc4p> Message-ID: Thank you guys, Thank you very much for your reply. I will try treatment of quenching at different time and find the correct time. Daivik ________________________________________ Daivik Shah, Histotechnologist, M.S. Biotechnology RADC Laboratory, Cohn Building 436 Rush University Medical Center Tel. 312-563-4891 This message and any attachments contain information for the exclusive use of the individual or entity to whom it is addressed and may contain information that is privileged, confidential and/or exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, or the employee or agent, you are hereby notified that any distribution or copying of this communication is strictly prohibited. If you received this message in error, please phone me immediately at 312-563-4891. "Joe Nocito" 09/26/2007 06:21 PM To "Ford, Judi" , "Rene J Buesa" , , cc Subject Re: [Histonet] Re: Hydrogen Peroxide quenching Judi, Judi Judi (sorry, couldn't help it) always, always, always. Did I mention always? Always do what is right for the lab you are in. I have tried H2O2 blocking before HIER, after HIER, after the secondary antibody and everywhere in between. As long as you block before the DAB you should be fine. Most of the time you can get away with what someone else is using. Sometimes it'll be a trial and error. Whatever works best in your situation Not every lab is the same and not every tech is the same. Your tissue processing might be different for the next lab, the temp & humidity in your lab may be different from the next lab. I know this may seem like a cop out, but it's true. When I was transferred from D.C. to San Antonio to set up an immuno lab, I had my former techs FedEx me some reagents to my new lab. Now, I had the same reagents, same procedures and same tech. I still had some adjustments to do in the new lab because I was getting a high, non-specific background staining. The environment was different as was the tissue processing. This is why my philosophy is there is no such thing as a pre-diluted antibody. Keep in mind that the companies perform THEIR testing in THEIR lab, not yours. I always try a short titer to see if I can get a 1:2 or a 1:4 dilution. Look it at it this way, if I can get a 1:4 dilution out of a pre-dilute, and the vial has 6 mls, I just purchased 24 mls for the cost of 6 mls (is that correct? Math was never my subject). Did ramble on again? Hey, not that this is a big thing, but I join the work force again on Monday. I'm keeping the place secret so I don't get called in by CEOs any more. JTT ----- Original Message ----- From: "Ford, Judi" To: "Rene J Buesa" ; ; Sent: Wednesday, September 26, 2007 5:03 PM Subject: RE: [Histonet] Re: Hydrogen Peroxide quenching Okay, so I'm confused. Some labs do the hydrogen peroxide step before HIER and others afterwards, why? Also, are there differences using hydrogen peroxide diluted in water/methanol/or PBS? Some bring paraffin slides down to absolute alcohol then do the hydrogen peroxide step where others wait until bringing the slides to water before doing the step. Is there some rule of thumb, or do you just figure out which procedure works for that specific antibody in your lab? I'm still pretty new to this, so sorry if this seems like a dumb question. Judi F. Palo Alto, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, September 26, 2007 2:49 PM To: Daivik_Shah@rush.edu; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Hydrogen Peroxide quenching You use it immediately after the HIER, before starting the IHC protocol. Ren? J. Daivik_Shah@rush.edu wrote: Hello friends, I have question regarding Hydrogen peroxide quenching. Is this treatment applied in Immunohistostain after deparafinization or before application of secondary antibody? Is that affect any way to stain? Thanks. Daivik _____________________________ Daivik Shah, Histotechnologist, M.S. Biotechnology RADC Laboratory, Cohn Building 436 Rush University Medical Center Tel. 312-563-4891 This message and any attachments contain information for the exclusive use of the individual or entity to whom it is addressed and may contain information that is privileged, confidential and/or exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, or the employee or agent, you are hereby notified that any distribution or copying of this communication is strictly prohibited. If you received this message in error, please phone me immediately at 312-563-4891. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Got a little couch potato? Check out fun summer activities for kids. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mlafrini <@t> csmlab.com Thu Sep 27 08:17:04 2007 From: mlafrini <@t> csmlab.com (Michael LaFriniere) Date: Thu Sep 27 08:16:13 2007 Subject: [Histonet] Manager Position Message-ID: <46FB748E.5898.00AF.0@csmlab.com> State of the art A/P laboratory located in Laural MD is seeking Histology Manager. Located 1/2 hour from DC, Baltimore and Annapolis. Excellent salary and benefits, great working conditions! For additional information Contact: Michael R. LaFriniere Executive Director Cytology Services of Maryland (CSM) 301-206-2555 ext 27 301-206-2595 fax michael.lafriniere@csmlab.com From dellav <@t> musc.edu Thu Sep 27 08:34:01 2007 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Thu Sep 27 08:35:06 2007 Subject: [Histonet] Dermatopathology question.... In-Reply-To: <968407.81746.qm@web50610.mail.re2.yahoo.com> References: <968407.81746.qm@web50610.mail.re2.yahoo.com> Message-ID: <7F6B678A32B0564196138E6B3101996AC48160@EVS1.clinlan.local> Kim, I can't speak to this with authority but it occurs to me that should the dermatology practice ever be sued by a patient who believes his case was mishandled in someway, I imagine the attorney's would have a field day with a physician who is not board certified reading out cases. If the individual is proficient, why isn't he/she boarded? Secondly, I would worry that billing out medicare patients would create potential problems with CMS if the case was read out by someone who is not boarded. I can't quote you chapter and verse of CMS regulations but it is impossible for me to believe that CMS is cool with paying physicians who charge for services when they aren't board certified in the specialty they are billing for. The whole issue speaks to who is and is not "qualified" to read out tissues and I would think one who is not board certified is not qualified in the eyes of just about everyone. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Tournear Sent: Thursday, September 27, 2007 8:57 AM To: Histonet Subject: [Histonet] Dermatopathology question.... Hi everyone, I work in a busy dermatology office. We process our own histology and do Mohs. We have 5 dermatologists and 3 P.A.'s. 1 of our dermatologists is a Board Certified Dermatopathologist. My question is: Since we have a Dermatopathologist in house, can a dermatologist, who is fluent in reading slides, (but not a certified as a dermatopathologist), read slides for another dermatologist (to help keep up)? Are there any legalities that I should be aware of? Any help would be greatly appreciated.....thanks in advance.... Kim Tournear, HT (ASCP), QIHC ( ASCP) Specialists in Dermatology Histology/Mohs Supervisor Tucson, AZ --------------------------------- Catch up on fall's hot new shows on Yahoo! TV. Watch previews, get listings, and more! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From medase <@t> leeds.ac.uk Thu Sep 27 08:46:18 2007 From: medase <@t> leeds.ac.uk (Alicia Sedo) Date: Thu Sep 27 08:46:32 2007 Subject: [Histonet] Losing sections. Message-ID: <1190900778.8986dc1ee220f@webmail6.leeds.ac.uk> Hello Histonet, I've been trying a Van Gieson stain on cryosections of mouse cardiac tissues, that have previously been stained for lacZ expression and are sitting in 4% PBS Formaldehyde. I have been losing the sections from the slides at the Van Gieson solution step. I am omitting the nuclear stain, instead going from washing in tap water to rinsing in distilled, then into the Van Gieson. I got the protocol (Staining of Collagen- on Frozen sections) from Histonet. The stain is 0.5% Aqueous Acid Fuchsin-15ml, Saturated Picric Acid-75ml, Distilled water-50ml...with 250microlitres of concentrated Nitric acid, which was recommended to sharpen the differentiation of the colours. The sections lift off and curl up after a few seconds, the slides are homemade silane coated and have withstood all other procedures so far. I have tried the stain without the Nitric acid but it still happens. I have previously tried this Van Gieson on FFPE cardiac tissue on the same slides, with good results. Am I missing something obvious that I don't know about? I am not a Histologist only a researcher in an University lab. I hope someone out there can point out what I am doing wrong! Alicia Sedo. Cardiovascular Research Level 11 Worsley Building, Clarendon Way University of Leeds. LS2-9JT. From doug <@t> ppspath.com Thu Sep 27 09:53:03 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Thu Sep 27 08:53:23 2007 Subject: {SPAM?} [Histonet] Dermatopathology question.... In-Reply-To: <968407.81746.qm@web50610.mail.re2.yahoo.com> Message-ID: Kim, You should place yourself in the position of the patient. Would you want your biopsy to be read by a non-certified dermpath? In the long run it may be cheaper to send the slides out vs. paying for an incorrect diagnosis. You can send them our way. We would be happy to help. :) Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Tournear Sent: Thursday, September 27, 2007 7:57 AM To: Histonet Subject: {SPAM?} [Histonet] Dermatopathology question.... Hi everyone, I work in a busy dermatology office. We process our own histology and do Mohs. We have 5 dermatologists and 3 P.A.'s. 1 of our dermatologists is a Board Certified Dermatopathologist. My question is: Since we have a Dermatopathologist in house, can a dermatologist, who is fluent in reading slides, (but not a certified as a dermatopathologist), read slides for another dermatologist (to help keep up)? Are there any legalities that I should be aware of? Any help would be greatly appreciated.....thanks in advance.... Kim Tournear, HT (ASCP), QIHC ( ASCP) Specialists in Dermatology Histology/Mohs Supervisor Tucson, AZ --------------------------------- Catch up on fall's hot new shows on Yahoo! TV. Watch previews, get listings, and more! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Heather.D.Renko <@t> osfhealthcare.org Thu Sep 27 09:03:44 2007 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Thu Sep 27 09:04:09 2007 Subject: [Histonet] Re: Instrumentation Message-ID: <40026EDDE64CDA47AB382C52619ACD3C06612D2C@pmc-rfd-mx01.intranet.osfnet.org> Hey friends, I know these have all been spoken about over and over but, I put a lot of efforts into my capital purchase dollars this year and I want a final personal opinion from my fellow histotechs out there on the following instrumentation: Autostainer: Really final on either a Leica or a Sakura Tissue Processor: Once again really looking at a Leica or a Sakura And last but, not least I am getting a cassette and slide labeling system that needs to interface well with Copath Plus/Misys Please reply to me personally with your opinions, I am making my final decisions at the NSH and want to get a feel for what the front line users think. Kind Regards to all-It is almost Friday! Heather Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From TJJ <@t> Stowers-Institute.org Thu Sep 27 09:12:40 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Thu Sep 27 09:13:06 2007 Subject: [Histonet] Re: Hydrogen Peroxide quenching Message-ID: Joe, I must respectfully differ with your advice: >>always, always, always. Did I mention always? Always do what is right for the lab you are in. I have tried H2O2 blocking before HIER, after HIER, after the secondary antibody and everywhere in between. As long as you block before the DAB you should be fine. Most of the time you can get away with what someone else is using. Sometimes it'll be a trial and error. Whatever works best in your situation<< If you block use H2O2 after the secondary antibody, you will quench the the HRP. I think you meant to say BEFORE the secondary antibody, not just anytime before the DAB. Some folks report needing to do the H2O2 quenching after HIER as it has been demonstrated to reactivate HRP if done prior to HIER. That has not been my experience, but because of this possibility, I do the quenching after HIER and before the protein block. Some folks are worried the H2O2 will negatively affect the binding of the antibody to their target, and therefore will not do the block until after the primary antibody incubation. If that makes them sleep better at night, then go for it. I don't think that aqueous H2O2 has as much an effect on immunoreactivity as solution using methanol. There are published reports where CD markers were negatively affected in cryosection using H2O2 in methanol as a block prior to primary antibody incubation. It may matter less in paraffin embedded material, but why take the risk? We use aqueous H2O2 and do not make it in PBS, only in distilled or DI water. Hope this helps! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From TJJ <@t> Stowers-Institute.org Thu Sep 27 09:26:29 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Thu Sep 27 09:26:47 2007 Subject: [Histonet] Re: Ohh the pain (replacing formalin) Message-ID: Douglas, if it is your Pathology group pushing you to go to non-formalin fixative, I'm quite surprised. Pathologists are accustomed to making diagnoses based on formalin-based artifacts. In a lab I worked in (in a galaxy far, far away) we did the "Folger's crystals replacement" experiment, replacing all the GI and prostate biopsy formalin fixative with zinc formalin. I thought the pathologists were going to have a major meltdown. The nucleoli were more prominent in all the cells, and that freaked them out. We effectively changed what the cellular structure looked like (even mild cellular changes can be a diagnostically significant). Disregarding the FDA issue and interlaboratory issues already raised (quite valid points!), if you change what fixative you are using, the cells will look different. And while that is not an insurmountable issue for pathologists, it does take getting used to. One possible substitute is glyoxal. Anatech provides this commercially in a fixative called "Prefer". It is supposed to provide formalin-like morphology with heightened immunoreactivity. Remember - ALL your immunohistochemistry staining will have to be redone. ALL OF IT. It is optimised using formalin, and changing the fixative changes the structure of the proteins. Some immunos may work better, some may not work as well, some may no longer require antigen retrieval, some may require a different retrieval method. Do you get any consultation material (outside blocks) from other institutions for second opinion? If so, and you need to do staining of any kind (H&E, special stains, and IHC), you will need to optimise your staining of that material back to formalin. Your non-formalin controls will be useless. I'm not saying this is an impossible task. It will be difficult and seem impossible until it's all worked out. It takes a tremendous amount of effort and energy to make the switch, and above all, the pathologists should have a major stake in how their samples will be fixed, processed, and stained. Their reputation depends on it. Their patient's diagnosis also depends on it. If you want to turn your attention to making formalin safer, there are ways of doing that. That's fodder for another post altogether. Good luck! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From kmerriam2003 <@t> yahoo.com Thu Sep 27 09:42:51 2007 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Thu Sep 27 09:42:56 2007 Subject: [Histonet] mucin stain on matrigel-coated cultured cells Message-ID: <370801.67828.qm@web50305.mail.re2.yahoo.com> Hello, Does anyone have a recommendation for a mucin stain for cultured cells on matrigel-coated slides? An investigator at my site is interested in this. I am worried that 1) the Schiff reagent will be too harsh for the cells and 2) that the matrigel itself might be PAS-positive. I think IHC or IF might be the way to go, instead of a special stain. Any thoughts? Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA ____________________________________________________________________________________ Moody friends. Drama queens. Your life? Nope! - their life, your story. Play Sims Stories at Yahoo! Games. http://sims.yahoo.com/ From kmerriam2003 <@t> yahoo.com Thu Sep 27 09:44:54 2007 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Thu Sep 27 09:44:59 2007 Subject: [Histonet] GDP or GLP Documentation Training Message-ID: <565667.42719.qm@web50304.mail.re2.yahoo.com> A couple of months ago, I came across a training site that specialized in this sort of thing; here is the website: http://www.cfpie.com/ Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA ----- Original Message ---- From: Liz Chlipala To: "Carter, Kendra" ; histonet@lists.utsouthwestern.edu Sent: Wednesday, September 26, 2007 12:43:33 PM Subject: RE: [Histonet] GDP or GLP Documentation Training Kendra We subcontract out our QA to a local company here in Boulder and they have also helped us with GLP training, etc. I would suspect that there are companies that are local to you that deal with regulatory issues that could help you out. Check to see if your state has a Bioscience association or something similar, if they do they will list vendors that can provide those types of support services. If you like I could contact my guys here and see if they know anyone that is in your area. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carter, Kendra Sent: Wednesday, September 26, 2007 9:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GDP or GLP Documentation Training Could anyone recommend any GDP or GLP Documentation Training for the Mid-Atlantic area? Thank you. Kendra Leigh Carter MedImmune, Inc. One MedImmune Way Gaithersburg, MD 20878 PH: 301-398-4956 Fax: 301-398-9956 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.5.488 / Virus Database: 269.13.30/1030 - Release Date: 9/25/2007 8:02 AM No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.5.488 / Virus Database: 269.13.30/1030 - Release Date: 9/25/2007 8:02 AM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Moody friends. Drama queens. Your life? Nope! - their life, your story. Play Sims Stories at Yahoo! Games. http://sims.yahoo.com/ From Barry.R.Rittman <@t> uth.tmc.edu Thu Sep 27 09:45:21 2007 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Thu Sep 27 09:45:24 2007 Subject: [Histonet] Re: Ohh the pain (replacing formalin) In-Reply-To: Message-ID: I know that we are all worried about formalin, however I think that we should all keep a sense of perspective here. While some things are obviously dangerous there are many more insidious dangers that we take for granted. Those of you that live in Houston will of course be aware of the air quality here. I like Houston a lot but on a still day it is sometimes a difficult choice between a short exposure to chemical fumes and the outside air. There are a tremendous number of dangers on the laboratory and I feel strongly that we should treat all chemicals as potential hazards as many of the newer chemicals have not been around long enough to determine their long term effects. It is not the fact that formalin, xylene, picric acid to name a few examples are dangerous but the manner in which we use them and the safety training of the personell involved. The major problem is that many people either do not have sufficient safety training or are sloppy around chemicals. I assume that this also carries over into their personal life so I would also not be comfortable eating food they have prepared. As has been pointed out, formalin is used universally as the gold standard and it will not be replaced overnight. Even if it were then how can we evaluate sections that were prepared several years ago? Let us be as safe as possible in use of these chemicals but let us also keep this problem in perspective. Thanks Barry From doug <@t> ppspath.com Thu Sep 27 11:15:15 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Thu Sep 27 10:15:37 2007 Subject: [Histonet] Re: Ohh the pain (replacing formalin) In-Reply-To: Message-ID: Teri, don't be surprised. Let's just say that I can not go into detail about the reasoning behind this. I am aware of all of the validation that is involved in this task. At least I hope I can get the "I told you so" in before I am kicked out the door. :) Douglas D. Deltour HT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Teri Sent: Thursday, September 27, 2007 9:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Ohh the pain (replacing formalin) Douglas, if it is your Pathology group pushing you to go to non-formalin fixative, I'm quite surprised. Pathologists are accustomed to making diagnoses based on formalin-based artifacts. In a lab I worked in (in a galaxy far, far away) we did the "Folger's crystals replacement" experiment, replacing all the GI and prostate biopsy formalin fixative with zinc formalin. I thought the pathologists were going to have a major meltdown. The nucleoli were more prominent in all the cells, and that freaked them out. We effectively changed what the cellular structure looked like (even mild cellular changes can be a diagnostically significant). Disregarding the FDA issue and interlaboratory issues already raised (quite valid points!), if you change what fixative you are using, the cells will look different. And while that is not an insurmountable issue for pathologists, it does take getting used to. One possible substitute is glyoxal. Anatech provides this commercially in a fixative called "Prefer". It is supposed to provide formalin-like morphology with heightened immunoreactivity. Remember - ALL your immunohistochemistry staining will have to be redone. ALL OF IT. It is optimised using formalin, and changing the fixative changes the structure of the proteins. Some immunos may work better, some may not work as well, some may no longer require antigen retrieval, some may require a different retrieval method. Do you get any consultation material (outside blocks) from other institutions for second opinion? If so, and you need to do staining of any kind (H&E, special stains, and IHC), you will need to optimise your staining of that material back to formalin. Your non-formalin controls will be useless. I'm not saying this is an impossible task. It will be difficult and seem impossible until it's all worked out. It takes a tremendous amount of effort and energy to make the switch, and above all, the pathologists should have a major stake in how their samples will be fixed, processed, and stained. Their reputation depends on it. Their patient's diagnosis also depends on it. If you want to turn your attention to making formalin safer, there are ways of doing that. That's fodder for another post altogether. Good luck! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Thu Sep 27 10:28:55 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Thu Sep 27 10:29:20 2007 Subject: [Histonet] Re: Ohh the pain (replacing formalin) In-Reply-To: References: Message-ID: Why not simply reduce the amount of formalin used, i.e. instead of 10% neutral buffered formalin use 5% or less, we used cold(4C) 4%NBF for one particular study, everything seemed to work OK. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: 27 September 2007 17:15 To: 'Johnson, Teri'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Ohh the pain (replacing formalin) Teri, don't be surprised. Let's just say that I can not go into detail about the reasoning behind this. I am aware of all of the validation that is involved in this task. At least I hope I can get the "I told you so" in before I am kicked out the door. :) Douglas D. Deltour HT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Teri Sent: Thursday, September 27, 2007 9:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Ohh the pain (replacing formalin) Douglas, if it is your Pathology group pushing you to go to non-formalin fixative, I'm quite surprised. Pathologists are accustomed to making diagnoses based on formalin-based artifacts. In a lab I worked in (in a galaxy far, far away) we did the "Folger's crystals replacement" experiment, replacing all the GI and prostate biopsy formalin fixative with zinc formalin. I thought the pathologists were going to have a major meltdown. The nucleoli were more prominent in all the cells, and that freaked them out. We effectively changed what the cellular structure looked like (even mild cellular changes can be a diagnostically significant). Disregarding the FDA issue and interlaboratory issues already raised (quite valid points!), if you change what fixative you are using, the cells will look different. And while that is not an insurmountable issue for pathologists, it does take getting used to. One possible substitute is glyoxal. Anatech provides this commercially in a fixative called "Prefer". It is supposed to provide formalin-like morphology with heightened immunoreactivity. Remember - ALL your immunohistochemistry staining will have to be redone. ALL OF IT. It is optimised using formalin, and changing the fixative changes the structure of the proteins. Some immunos may work better, some may not work as well, some may no longer require antigen retrieval, some may require a different retrieval method. Do you get any consultation material (outside blocks) from other institutions for second opinion? If so, and you need to do staining of any kind (H&E, special stains, and IHC), you will need to optimise your staining of that material back to formalin. Your non-formalin controls will be useless. I'm not saying this is an impossible task. It will be difficult and seem impossible until it's all worked out. It takes a tremendous amount of effort and energy to make the switch, and above all, the pathologists should have a major stake in how their samples will be fixed, processed, and stained. Their reputation depends on it. Their patient's diagnosis also depends on it. If you want to turn your attention to making formalin safer, there are ways of doing that. That's fodder for another post altogether. Good luck! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dellav <@t> musc.edu Thu Sep 27 10:36:03 2007 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Thu Sep 27 10:38:05 2007 Subject: [Histonet] Re: Ohh the pain (replacing formalin) In-Reply-To: References: Message-ID: <7F6B678A32B0564196138E6B3101996AC48299@EVS1.clinlan.local> While this may work for fixation this would not eliminate the stringent federal regulatory requirements here in the states which I assume is partially what Doug's lab is attempting to avoid. Any solutions containing .1% or greater formaldehyde are regulated by OSHA. 10% formalin is equivalent to 3.7% formaldehyde. 5% formalin would be equivalent to 1.9% formaldehyde, well over the regulated level. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, R.E. Sent: Thursday, September 27, 2007 11:29 AM To: Douglas D Deltour; Johnson, Teri; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Ohh the pain (replacing formalin) Why not simply reduce the amount of formalin used, i.e. instead of 10% neutral buffered formalin use 5% or less, we used cold(4C) 4%NBF for one particular study, everything seemed to work OK. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: 27 September 2007 17:15 To: 'Johnson, Teri'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Ohh the pain (replacing formalin) Teri, don't be surprised. Let's just say that I can not go into detail about the reasoning behind this. I am aware of all of the validation that is involved in this task. At least I hope I can get the "I told you so" in before I am kicked out the door. :) Douglas D. Deltour HT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Teri Sent: Thursday, September 27, 2007 9:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Ohh the pain (replacing formalin) Douglas, if it is your Pathology group pushing you to go to non-formalin fixative, I'm quite surprised. Pathologists are accustomed to making diagnoses based on formalin-based artifacts. In a lab I worked in (in a galaxy far, far away) we did the "Folger's crystals replacement" experiment, replacing all the GI and prostate biopsy formalin fixative with zinc formalin. I thought the pathologists were going to have a major meltdown. The nucleoli were more prominent in all the cells, and that freaked them out. We effectively changed what the cellular structure looked like (even mild cellular changes can be a diagnostically significant). Disregarding the FDA issue and interlaboratory issues already raised (quite valid points!), if you change what fixative you are using, the cells will look different. And while that is not an insurmountable issue for pathologists, it does take getting used to. One possible substitute is glyoxal. Anatech provides this commercially in a fixative called "Prefer". It is supposed to provide formalin-like morphology with heightened immunoreactivity. Remember - ALL your immunohistochemistry staining will have to be redone. ALL OF IT. It is optimised using formalin, and changing the fixative changes the structure of the proteins. Some immunos may work better, some may not work as well, some may no longer require antigen retrieval, some may require a different retrieval method. Do you get any consultation material (outside blocks) from other institutions for second opinion? If so, and you need to do staining of any kind (H&E, special stains, and IHC), you will need to optimise your staining of that material back to formalin. Your non-formalin controls will be useless. I'm not saying this is an impossible task. It will be difficult and seem impossible until it's all worked out. It takes a tremendous amount of effort and energy to make the switch, and above all, the pathologists should have a major stake in how their samples will be fixed, processed, and stained. Their reputation depends on it. Their patient's diagnosis also depends on it. If you want to turn your attention to making formalin safer, there are ways of doing that. That's fodder for another post altogether. Good luck! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LeslieS <@t> vetmed.ufl.edu Thu Sep 27 10:45:27 2007 From: LeslieS <@t> vetmed.ufl.edu (Shawn Leslie) Date: Thu Sep 27 10:45:58 2007 Subject: [Histonet] Re: Ohh the pain (replacing formalin) In-Reply-To: References: Message-ID: <46FB97DC.CC0E.007B.0@vetmed.ufl.edu> It's a real hassle changing from either...We had originally used Anatec's Prefer but because HER2Neu is acceptable only on formalin fixed tissue we had to change to formalin...that's when the nightmares began...Everything had to be work up again, H&E..specials, immunos......we had warned the Doc's that there will be reduced quality using formalin instead of Prefer but were told that we had to change and that they will have to accept the quality difference...that was fine and good until the first batch of slides came out and they freaked.....we were told to try this and to try that.....after a while no one knew what was going on because procedural changes were being done on an hourly basis....You could imagine the chaos in the lab.....All I can suggest is that if you have to change either way do it in a organized, systematic and scientific way otherwise everyone will regret it ...I guarantee it..... Shawn Leslie Scientific Research Manager Anatomic Pathology University of Florida School of Veterinary Medicine 352-392-2235 ext 4555 >>> "Douglas D Deltour" 9/27/2007 12:15 PM >>> Teri, don't be surprised. Let's just say that I can not go into detail about the reasoning behind this. I am aware of all of the validation that is involved in this task. At least I hope I can get the "I told you so" in before I am kicked out the door. :) Douglas D. Deltour HT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Teri Sent: Thursday, September 27, 2007 9:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Ohh the pain (replacing formalin) Douglas, if it is your Pathology group pushing you to go to non-formalin fixative, I'm quite surprised. Pathologists are accustomed to making diagnoses based on formalin-based artifacts. In a lab I worked in (in a galaxy far, far away) we did the "Folger's crystals replacement" experiment, replacing all the GI and prostate biopsy formalin fixative with zinc formalin. I thought the pathologists were going to have a major meltdown. The nucleoli were more prominent in all the cells, and that freaked them out. We effectively changed what the cellular structure looked like (even mild cellular changes can be a diagnostically significant). Disregarding the FDA issue and interlaboratory issues already raised (quite valid points!), if you change what fixative you are using, the cells will look different. And while that is not an insurmountable issue for pathologists, it does take getting used to. One possible substitute is glyoxal. Anatech provides this commercially in a fixative called "Prefer". It is supposed to provide formalin-like morphology with heightened immunoreactivity. Remember - ALL your immunohistochemistry staining will have to be redone. ALL OF IT. It is optimised using formalin, and changing the fixative changes the structure of the proteins. Some immunos may work better, some may not work as well, some may no longer require antigen retrieval, some may require a different retrieval method. Do you get any consultation material (outside blocks) from other institutions for second opinion? If so, and you need to do staining of any kind (H&E, special stains, and IHC), you will need to optimise your staining of that material back to formalin. Your non-formalin controls will be useless. I'm not saying this is an impossible task. It will be difficult and seem impossible until it's all worked out. It takes a tremendous amount of effort and energy to make the switch, and above all, the pathologists should have a major stake in how their samples will be fixed, processed, and stained. Their reputation depends on it. Their patient's diagnosis also depends on it. If you want to turn your attention to making formalin safer, there are ways of doing that. That's fodder for another post altogether. Good luck! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Thu Sep 27 12:02:27 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Thu Sep 27 11:03:04 2007 Subject: [Histonet] Re: Ohh the pain (replacing formalin) In-Reply-To: Message-ID: It isn't really about decreasing formalin contact or staff safety. It is about being labeled as a "green lab". Douglas D. Deltour HT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, R.E. Sent: Thursday, September 27, 2007 10:29 AM To: Douglas D Deltour; Johnson, Teri; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Ohh the pain (replacing formalin) Why not simply reduce the amount of formalin used, i.e. instead of 10% neutral buffered formalin use 5% or less, we used cold(4C) 4%NBF for one particular study, everything seemed to work OK. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: 27 September 2007 17:15 To: 'Johnson, Teri'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Ohh the pain (replacing formalin) Teri, don't be surprised. Let's just say that I can not go into detail about the reasoning behind this. I am aware of all of the validation that is involved in this task. At least I hope I can get the "I told you so" in before I am kicked out the door. :) Douglas D. Deltour HT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Teri Sent: Thursday, September 27, 2007 9:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Ohh the pain (replacing formalin) Douglas, if it is your Pathology group pushing you to go to non-formalin fixative, I'm quite surprised. Pathologists are accustomed to making diagnoses based on formalin-based artifacts. In a lab I worked in (in a galaxy far, far away) we did the "Folger's crystals replacement" experiment, replacing all the GI and prostate biopsy formalin fixative with zinc formalin. I thought the pathologists were going to have a major meltdown. The nucleoli were more prominent in all the cells, and that freaked them out. We effectively changed what the cellular structure looked like (even mild cellular changes can be a diagnostically significant). Disregarding the FDA issue and interlaboratory issues already raised (quite valid points!), if you change what fixative you are using, the cells will look different. And while that is not an insurmountable issue for pathologists, it does take getting used to. One possible substitute is glyoxal. Anatech provides this commercially in a fixative called "Prefer". It is supposed to provide formalin-like morphology with heightened immunoreactivity. Remember - ALL your immunohistochemistry staining will have to be redone. ALL OF IT. It is optimised using formalin, and changing the fixative changes the structure of the proteins. Some immunos may work better, some may not work as well, some may no longer require antigen retrieval, some may require a different retrieval method. Do you get any consultation material (outside blocks) from other institutions for second opinion? If so, and you need to do staining of any kind (H&E, special stains, and IHC), you will need to optimise your staining of that material back to formalin. Your non-formalin controls will be useless. I'm not saying this is an impossible task. It will be difficult and seem impossible until it's all worked out. It takes a tremendous amount of effort and energy to make the switch, and above all, the pathologists should have a major stake in how their samples will be fixed, processed, and stained. Their reputation depends on it. Their patient's diagnosis also depends on it. If you want to turn your attention to making formalin safer, there are ways of doing that. That's fodder for another post altogether. Good luck! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Sep 27 11:08:10 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 27 11:08:14 2007 Subject: [Histonet] Losing sections. In-Reply-To: <1190900778.8986dc1ee220f@webmail6.leeds.ac.uk> Message-ID: <350966.62950.qm@web61216.mail.yahoo.com> Ttry it again after eliminating the nitric acid (not really needed). Ren? J. Alicia Sedo wrote: Hello Histonet, I've been trying a Van Gieson stain on cryosections of mouse cardiac tissues, that have previously been stained for lacZ expression and are sitting in 4% PBS Formaldehyde. I have been losing the sections from the slides at the Van Gieson solution step. I am omitting the nuclear stain, instead going from washing in tap water to rinsing in distilled, then into the Van Gieson. I got the protocol (Staining of Collagen- on Frozen sections) from Histonet. The stain is 0.5% Aqueous Acid Fuchsin-15ml, Saturated Picric Acid-75ml, Distilled water-50ml...with 250microlitres of concentrated Nitric acid, which was recommended to sharpen the differentiation of the colours. The sections lift off and curl up after a few seconds, the slides are homemade silane coated and have withstood all other procedures so far. I have tried the stain without the Nitric acid but it still happens. I have previously tried this Van Gieson on FFPE cardiac tissue on the same slides, with good results. Am I missing something obvious that I don't know about? I am not a Histologist only a researcher in an University lab. I hope someone out there can point out what I am doing wrong! Alicia Sedo. Cardiovascular Research Level 11 Worsley Building, Clarendon Way University of Leeds. LS2-9JT. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Moody friends. Drama queens. Your life? Nope! - their life, your story. Play Sims Stories at Yahoo! Games. From Boneslides <@t> aol.com Thu Sep 27 11:09:35 2007 From: Boneslides <@t> aol.com (Boneslides@aol.com) Date: Thu Sep 27 11:10:00 2007 Subject: [Histonet] Re-embedding JB4 plastic specimens Message-ID: Is it possible to re-embed specimens that have been embedded in JB4 plastic (GMA)? The specimens moved during polymerization under vacuum and the orientation is less than optimal. Protocols for a procedure would be most appreciated. Thanks in advance for your help! ~Diane Diane M. Mahovlic, HT, MLT(ASCP) Orthopedic Pathology & Biomaterials Laboratory Department of Anatomic Pathology The Cleveland Clinic Foundation 9500 Euclid Avenue- L30 Cleveland, Ohio 44195 216-444-0166 ************************************** See what's new at http://www.aol.com From rjbuesa <@t> yahoo.com Thu Sep 27 11:10:03 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 27 11:10:09 2007 Subject: [Histonet] Re: Instrumentation In-Reply-To: <40026EDDE64CDA47AB382C52619ACD3C06612D2C@pmc-rfd-mx01.intranet.osfnet.org> Message-ID: <843496.98839.qm@web61216.mail.yahoo.com> Autostainer and tissue processor: Sakura. Ren? J. "Renko, Heather D." wrote: Hey friends, I know these have all been spoken about over and over but, I put a lot of efforts into my capital purchase dollars this year and I want a final personal opinion from my fellow histotechs out there on the following instrumentation: Autostainer: Really final on either a Leica or a Sakura Tissue Processor: Once again really looking at a Leica or a Sakura And last but, not least I am getting a cassette and slide labeling system that needs to interface well with Copath Plus/Misys Please reply to me personally with your opinions, I am making my final decisions at the NSH and want to get a feel for what the front line users think. Kind Regards to all-It is almost Friday! Heather Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better Globetrotter. Get better travel answers from someone who knows. Yahoo! Answers - Check it out. From victor <@t> pathology.washington.edu Thu Sep 27 11:13:45 2007 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Thu Sep 27 11:14:41 2007 Subject: [Histonet] Re: Ohh the pain (replacing formalin) Message-ID: <46FBD6B9.2050807@pathology.washington.edu> I find this discussion very interesting. I haven't worked in the lab in awhile, so would the use of microwaves and their proprietary solutions be a step in the right direction? Part of the problem is not knowing what is in their reagents. Victor Douglas D Deltour wrote: > It isn't really about decreasing formalin contact or staff safety. It is > about being labeled as a "green lab". > > Douglas D. Deltour HT(ASCP) > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, > R.E. > Sent: Thursday, September 27, 2007 10:29 AM > To: Douglas D Deltour; Johnson, Teri; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Ohh the pain (replacing formalin) > > Why not simply reduce the amount of formalin used, i.e. instead > of 10% neutral buffered formalin use 5% or less, we used cold(4C) > 4%NBF for one particular study, everything seemed to work OK. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas > D Deltour > Sent: 27 September 2007 17:15 > To: 'Johnson, Teri'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Ohh the pain (replacing formalin) > > Teri, don't be surprised. Let's just say that I can not go into detail > about the reasoning behind this. I am aware of all of the validation > that is involved in this task. At least I hope I can get the "I told you > so" in > before I am kicked out the door. :) > > > Douglas D. Deltour HT(ASCP) > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, > Teri > Sent: Thursday, September 27, 2007 9:26 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Ohh the pain (replacing formalin) > > Douglas, if it is your Pathology group pushing you to go to non-formalin > fixative, I'm quite surprised. Pathologists are accustomed to making > diagnoses based on formalin-based artifacts. In a lab I worked in (in a > galaxy far, far away) we did the "Folger's crystals replacement" > experiment, replacing all the GI and prostate biopsy formalin fixative > with zinc formalin. I thought the pathologists were going to have a > major meltdown. The nucleoli were more prominent in all the cells, and > that freaked them out. We effectively changed what the cellular > structure looked like (even mild cellular changes can be a > diagnostically significant). > > Disregarding the FDA issue and interlaboratory issues already raised > (quite valid points!), if you change what fixative you are using, the > cells will look different. And while that is not an insurmountable issue > for pathologists, it does take getting used to. > > One possible substitute is glyoxal. Anatech provides this commercially > in a fixative called "Prefer". It is supposed to provide formalin-like > morphology with heightened immunoreactivity. Remember - ALL your > immunohistochemistry staining will have to be redone. ALL OF IT. It is > optimised using formalin, and changing the fixative changes the > structure of the proteins. Some immunos may work better, some may not > work as well, some may no longer require antigen retrieval, some may > require a different retrieval method. > > Do you get any consultation material (outside blocks) from other > institutions for second opinion? If so, and you need to do staining of > any kind (H&E, special stains, and IHC), you will need to optimise your > staining of that material back to formalin. Your non-formalin controls > will be useless. > > I'm not saying this is an impossible task. It will be difficult and seem > impossible until it's all worked out. It takes a tremendous amount of > effort and energy to make the switch, and above all, the pathologists > should have a major stake in how their samples will be fixed, processed, > and stained. Their reputation depends on it. Their patient's diagnosis > also depends on it. > > If you want to turn your attention to making formalin safer, there are > ways of doing that. That's fodder for another post altogether. > > Good luck! > > Teri Johnson, HT(ASCP)QIHC > Managing Director Histology Facility > Stowers Institute for Medical Research > 1000 E. 50th St. > Kansas City, MO 64110 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From rjbuesa <@t> yahoo.com Thu Sep 27 11:19:35 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 27 11:19:38 2007 Subject: Fwd: RE: [Histonet] Re: Hydrogen Peroxide quenching Message-ID: <163188.41717.qm@web61220.mail.yahoo.com> Rene J Buesa wrote: Date: Thu, 27 Sep 2007 08:51:51 -0700 (PDT) From: Rene J Buesa Subject: RE: [Histonet] Re: Hydrogen Peroxide quenching To: "Ford, Judi" Judi: Do not be confused at all. This is what happens when working in an unregulated specialty where procedures, like cooking recipes, vary from chef to chef even when all are making "chicken parmigiani". Histology lacks strict methodologies and each one dances to his/her own tune. People will tell you what they do, and sometimes there is no technical backing for the selection. Quenching the omnipresent tissue peroxidase is a requirement to prevent backgound noise. HIER is required to unmask the epitopes and after that step is when I used to quench. Even I did that BEFORE starting the autostainer and by doing so I was able to quench a complete 48 slides run in 5 minutes. cutting 25 minutes from the protocol had this step being included in the automatic run. My procedure deviates from other procedures as those other deviate from mine. Make you pick but be consistent in how you do it. I hope I have not added more confusion! Ren? J. "Ford, Judi" wrote: Okay, so I'm confused. Some labs do the hydrogen peroxide step before HIER and others afterwards, why? Also, are there differences using hydrogen peroxide diluted in water/methanol/or PBS? Some bring paraffin slides down to absolute alcohol then do the hydrogen peroxide step where others wait until bringing the slides to water before doing the step. Is there some rule of thumb, or do you just figure out which procedure works for that specific antibody in your lab? I'm still pretty new to this, so sorry if this seems like a dumb question. Judi F. Palo Alto, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, September 26, 2007 2:49 PM To: Daivik_Shah@rush.edu; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Hydrogen Peroxide quenching You use it immediately after the HIER, before starting the IHC protocol. Ren? J. Daivik_Shah@rush.edu wrote: Hello friends, I have question regarding Hydrogen peroxide quenching. Is this treatment applied in Immunohistostain after deparafinization or before application of secondary antibody? Is that affect any way to stain? Thanks. Daivik _____________________________ Daivik Shah, Histotechnologist, M.S. Biotechnology RADC Laboratory, Cohn Building 436 Rush University Medical Center Tel. 312-563-4891 This message and any attachments contain information for the exclusive use of the individual or entity to whom it is addressed and may contain information that is privileged, confidential and/or exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, or the employee or agent, you are hereby notified that any distribution or copying of this communication is strictly prohibited. If you received this message in error, please phone me immediately at 312-563-4891. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Got a little couch potato? Check out fun summer activities for kids. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Luggage? GPS? Comic books? Check out fitting gifts for grads at Yahoo! Search. --------------------------------- Luggage? GPS? Comic books? Check out fitting gifts for grads at Yahoo! Search. From MBURTON1 <@t> PARTNERS.ORG Thu Sep 27 11:23:44 2007 From: MBURTON1 <@t> PARTNERS.ORG (Burton, Mark) Date: Thu Sep 27 11:23:58 2007 Subject: [Histonet] Histology on YouTube Message-ID: Not sure how many of you visit youtube.com but I thought I should share this. Here is a link to some short basic videos reviewing histology of some major organs and structures. Feel free to criticize or praise (I don't know the narrator) but remember it's free! http://www.youtube.com/profile_videos?user=WashingtonDeceit&p=r Mark Burton HTL ASCP Brigham & Women's Hospital Boston, MA The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From cmiller <@t> physlab.com Thu Sep 27 11:28:34 2007 From: cmiller <@t> physlab.com (Cheri Miller) Date: Thu Sep 27 11:28:42 2007 Subject: [Histonet] Dermatopathology question.... In-Reply-To: <968407.81746.qm@web50610.mail.re2.yahoo.com> References: <968407.81746.qm@web50610.mail.re2.yahoo.com> Message-ID: <002401c80123$74d94ba0$3402a8c0@plab.local> Is he/she a pathologist at all??. Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Tournear Sent: Thursday, September 27, 2007 7:57 AM To: Histonet Subject: [Histonet] Dermatopathology question.... Hi everyone, I work in a busy dermatology office. We process our own histology and do Mohs. We have 5 dermatologists and 3 P.A.'s. 1 of our dermatologists is a Board Certified Dermatopathologist. My question is: Since we have a Dermatopathologist in house, can a dermatologist, who is fluent in reading slides, (but not a certified as a dermatopathologist), read slides for another dermatologist (to help keep up)? Are there any legalities that I should be aware of? Any help would be greatly appreciated.....thanks in advance.... Kim Tournear, HT (ASCP), QIHC ( ASCP) Specialists in Dermatology Histology/Mohs Supervisor Tucson, AZ --------------------------------- Catch up on fall's hot new shows on Yahoo! TV. Watch previews, get listings, and more! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From Lynn.Burton <@t> Illinois.gov Thu Sep 27 11:40:27 2007 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Thu Sep 27 11:41:27 2007 Subject: [Histonet] Re: Instrumentation References: <843496.98839.qm@web61216.mail.yahoo.com> Message-ID: both stainer and processor SAKURA Lynn Burton ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Thu 9/27/2007 11:10 AM To: Renko, Heather D.; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Instrumentation Autostainer and tissue processor: Sakura. Ren? J. "Renko, Heather D." wrote: Hey friends, I know these have all been spoken about over and over but, I put a lot of efforts into my capital purchase dollars this year and I want a final personal opinion from my fellow histotechs out there on the following instrumentation: Autostainer: Really final on either a Leica or a Sakura Tissue Processor: Once again really looking at a Leica or a Sakura And last but, not least I am getting a cassette and slide labeling system that needs to interface well with Copath Plus/Misys Please reply to me personally with your opinions, I am making my final decisions at the NSH and want to get a feel for what the front line users think. Kind Regards to all-It is almost Friday! Heather Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better Globetrotter. Get better travel answers from someone who knows. Yahoo! Answers - Check it out. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Thu Sep 27 12:43:01 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Thu Sep 27 11:43:26 2007 Subject: {SPAM?} Re: [Histonet] Re: Ohh the pain (replacing formalin) In-Reply-To: <46FBD6B9.2050807@pathology.washington.edu> Message-ID: Not all microwaves use proprietary solutions. We use the Milestone Pathos. We still can use 10% NBF as the fixative. I can see some of the same issues if a laboratory decided to go in the direction of a proprietary fixative based processor. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Thursday, September 27, 2007 11:14 AM To: Douglas D Deltour Cc: histonet@lists.utsouthwestern.edu Subject: {SPAM?} Re: [Histonet] Re: Ohh the pain (replacing formalin) I find this discussion very interesting. I haven't worked in the lab in awhile, so would the use of microwaves and their proprietary solutions be a step in the right direction? Part of the problem is not knowing what is in their reagents. Victor Douglas D Deltour wrote: > It isn't really about decreasing formalin contact or staff safety. It is > about being labeled as a "green lab". > > Douglas D. Deltour HT(ASCP) > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, > R.E. > Sent: Thursday, September 27, 2007 10:29 AM > To: Douglas D Deltour; Johnson, Teri; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Ohh the pain (replacing formalin) > > Why not simply reduce the amount of formalin used, i.e. instead > of 10% neutral buffered formalin use 5% or less, we used cold(4C) > 4%NBF for one particular study, everything seemed to work OK. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas > D Deltour > Sent: 27 September 2007 17:15 > To: 'Johnson, Teri'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Ohh the pain (replacing formalin) > > Teri, don't be surprised. Let's just say that I can not go into detail > about the reasoning behind this. I am aware of all of the validation > that is involved in this task. At least I hope I can get the "I told you > so" in > before I am kicked out the door. :) > > > Douglas D. Deltour HT(ASCP) > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, > Teri > Sent: Thursday, September 27, 2007 9:26 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Ohh the pain (replacing formalin) > > Douglas, if it is your Pathology group pushing you to go to non-formalin > fixative, I'm quite surprised. Pathologists are accustomed to making > diagnoses based on formalin-based artifacts. In a lab I worked in (in a > galaxy far, far away) we did the "Folger's crystals replacement" > experiment, replacing all the GI and prostate biopsy formalin fixative > with zinc formalin. I thought the pathologists were going to have a > major meltdown. The nucleoli were more prominent in all the cells, and > that freaked them out. We effectively changed what the cellular > structure looked like (even mild cellular changes can be a > diagnostically significant). > > Disregarding the FDA issue and interlaboratory issues already raised > (quite valid points!), if you change what fixative you are using, the > cells will look different. And while that is not an insurmountable issue > for pathologists, it does take getting used to. > > One possible substitute is glyoxal. Anatech provides this commercially > in a fixative called "Prefer". It is supposed to provide formalin-like > morphology with heightened immunoreactivity. Remember - ALL your > immunohistochemistry staining will have to be redone. ALL OF IT. It is > optimised using formalin, and changing the fixative changes the > structure of the proteins. Some immunos may work better, some may not > work as well, some may no longer require antigen retrieval, some may > require a different retrieval method. > > Do you get any consultation material (outside blocks) from other > institutions for second opinion? If so, and you need to do staining of > any kind (H&E, special stains, and IHC), you will need to optimise your > staining of that material back to formalin. Your non-formalin controls > will be useless. > > I'm not saying this is an impossible task. It will be difficult and seem > impossible until it's all worked out. It takes a tremendous amount of > effort and energy to make the switch, and above all, the pathologists > should have a major stake in how their samples will be fixed, processed, > and stained. Their reputation depends on it. Their patient's diagnosis > also depends on it. > > If you want to turn your attention to making formalin safer, there are > ways of doing that. That's fodder for another post altogether. > > Good luck! > > Teri Johnson, HT(ASCP)QIHC > Managing Director Histology Facility > Stowers Institute for Medical Research > 1000 E. 50th St. > Kansas City, MO 64110 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jmahoney <@t> alegent.org Thu Sep 27 12:27:04 2007 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Thu Sep 27 12:27:24 2007 Subject: [Histonet] Dermatopathology question.... In-Reply-To: <7F6B678A32B0564196138E6B3101996AC48160@EVS1.clinlan.local> References: <968407.81746.qm@web50610.mail.re2.yahoo.com> <7F6B678A32B0564196138E6B3101996AC48160@EVS1.clinlan.local> Message-ID: <46FBA1980200003C0001B662@gwia.alegent.org> I find this topic very interesting. I used to think that only pathologists could read slides. I'm no expert either but I do know that most dermatologists read their own slides and send anything that is not straight forward on to a cetified pathologist. I'm going to have to look up the CLIA requirements and educate myself. I agree with Vinnie, I'd rather have a Pathologist diagnosing my slides. Jan Omah >>> "Della Speranza, Vinnie" 09/27/2007 8:34 AM >>> Kim, I can't speak to this with authority but it occurs to me that should the dermatology practice ever be sued by a patient who believes his case was mishandled in someway, I imagine the attorney's would have a field day with a physician who is not board certified reading out cases. If the individual is proficient, why isn't he/she boarded? Secondly, I would worry that billing out medicare patients would create potential problems with CMS if the case was read out by someone who is not boarded. I can't quote you chapter and verse of CMS regulations but it is impossible for me to believe that CMS is cool with paying physicians who charge for services when they aren't board certified in the specialty they are billing for. The whole issue speaks to who is and is not "qualified" to read out tissues and I would think one who is not board certified is not qualified in the eyes of just about everyone. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Tournear Sent: Thursday, September 27, 2007 8:57 AM To: Histonet Subject: [Histonet] Dermatopathology question.... Hi everyone, I work in a busy dermatology office. We process our own histology and do Mohs. We have 5 dermatologists and 3 P.A.'s. 1 of our dermatologists is a Board Certified Dermatopathologist. My question is: Since we have a Dermatopathologist in house, can a dermatologist, who is fluent in reading slides, (but not a certified as a dermatopathologist), read slides for another dermatologist (to help keep up)? Are there any legalities that I should be aware of? Any help would be greatly appreciated.....thanks in advance.... Kim Tournear, HT (ASCP), QIHC ( ASCP) Specialists in Dermatology Histology/Mohs Supervisor Tucson, AZ --------------------------------- Catch up on fall's hot new shows on Yahoo! TV. Watch previews, get listings, and more! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mlafrini <@t> csmlab.com Thu Sep 27 13:12:13 2007 From: mlafrini <@t> csmlab.com (Michael LaFriniere) Date: Thu Sep 27 13:12:30 2007 Subject: [Histonet] Dermatopathology question.... In-Reply-To: <46FBA1980200003C0001B662@gwia.alegent.org> References: <968407.81746.qm@web50610.mail.re2.yahoo.com> <7F6B678A32B0564196138E6B3101996AC48160@EVS1.clinlan.local> <46FBA1980200003C0001B662@gwia.alegent.org> Message-ID: <46FBB9B9.5898.00AF.0@csmlab.com> It has been my experience that most Dermatologists have had sufficient training to read histological sections, however, this does place liability issues on their practice, and this is why many dermatologist choose to have a pathologist (preferably a dermatopathologist) read their patients' specimens. In this part of the country, I am noticing that approx 50% of dermatologists are reading their own cases, and sending difficult cases, i.e., cancers, melanoma's or any melanocytic lesions to a dermatopathologist. The dermatologist can charge the pathology professional fee for performing the histological professional component. Michael R. LaFriniere Executive Director Cytology Services of Maryland (CSM) 301-206-2555 ext 27 301-206-2595 fax michael.lafriniere@csmlab.com >>> On 9/27/2007 at 1:27 PM, in message <46FBA1980200003C0001B662@gwia.alegent.org>, "Janice Mahoney" wrote: I find this topic very interesting. I used to think that only pathologists could read slides. I'm no expert either but I do know that most dermatologists read their own slides and send anything that is not straight forward on to a cetified pathologist. I'm going to have to look up the CLIA requirements and educate myself. I agree with Vinnie, I'd rather have a Pathologist diagnosing my slides. Jan Omah >>> "Della Speranza, Vinnie" 09/27/2007 8:34 AM >>> Kim, I can't speak to this with authority but it occurs to me that should the dermatology practice ever be sued by a patient who believes his case was mishandled in someway, I imagine the attorney's would have a field day with a physician who is not board certified reading out cases. If the individual is proficient, why isn't he/she boarded? Secondly, I would worry that billing out medicare patients would create potential problems with CMS if the case was read out by someone who is not boarded. I can't quote you chapter and verse of CMS regulations but it is impossible for me to believe that CMS is cool with paying physicians who charge for services when they aren't board certified in the specialty they are billing for. The whole issue speaks to who is and is not "qualified" to read out tissues and I would think one who is not board certified is not qualified in the eyes of just about everyone. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Tournear Sent: Thursday, September 27, 2007 8:57 AM To: Histonet Subject: [Histonet] Dermatopathology question.... Hi everyone, I work in a busy dermatology office. We process our own histology and do Mohs. We have 5 dermatologists and 3 P.A.'s. 1 of our dermatologists is a Board Certified Dermatopathologist. My question is: Since we have a Dermatopathologist in house, can a dermatologist, who is fluent in reading slides, (but not a certified as a dermatopathologist), read slides for another dermatologist (to help keep up)? Are there any legalities that I should be aware of? Any help would be greatly appreciated.....thanks in advance.... Kim Tournear, HT (ASCP), QIHC ( ASCP) Specialists in Dermatology Histology/Mohs Supervisor Tucson, AZ --------------------------------- Catch up on fall's hot new shows on Yahoo! TV. Watch previews, get listings, and more! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Thu Sep 27 13:33:55 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Thu Sep 27 13:34:33 2007 Subject: [Histonet] Re: Re-embedding JB4 plastic Message-ID: <000701c80134$f65f5870$4101a8c0@carlba65530bda> Yes , it's relatively easy, tho'' fiddly, if you have small specimens: I do it whenever I have specimens ( like fruitflies, zebrafish tiddlers) that I need to cut T/S - they NEVER stand up on their own! I embed them flat , about a mm from the shortest side of the mold. After polymerisation, use a junior hacksaw to cut away excess resin in such a way as to make a flat face of the side you want to embed on, ensuring that it will fit into your mold.( for fine further fine trimming I'll use a safety blade ) Clean away, in abs alc, resin debri, dry and then place in final stage GMA infiltrating resin for a while, on a rocker. I give fruitflies a couple of hours, for the heck of it. Then make up your embedding resin, place the trimmed block in the mold, add the resin....... Never failed me yet NB: if you are placing a chuck on top of your resin mold, to fix the polymerised block onto it, ready for cutting, I wouldn't bother polymerising under vacuum. Good luck. If you wish, you can have a look at a couple of sections of wholemount NISH specimens that have been subsequently processed to GMA resin, in the way outlined above: http://www.immunoportal.com/ in the In situ pic gallery. I hope this is apt/helpful. Carl From carl.hobbs <@t> kcl.ac.uk Thu Sep 27 13:58:54 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Thu Sep 27 13:59:31 2007 Subject: [Histonet] Re: Sorry for my error Message-ID: <002001c80138$73b75450$4101a8c0@carlba65530bda> It's such a pain receiving a complete previous post, from an established member, sigh! Contrite Carl From blord <@t> swmail.sw.org Thu Sep 27 14:07:18 2007 From: blord <@t> swmail.sw.org (Barbara Lord) Date: Thu Sep 27 14:07:26 2007 Subject: [Histonet] Dermpath ??? Message-ID: I ask my Dermatologist and he said CLIA reg that a board certified dermatologist can read his own slides. He also said though the dermatologist should be listed as laboratory personnal. Hope this helps Barbara Lord Mohs Technician Scott & White Dermatology Dept Temple , TX From rjbuesa <@t> yahoo.com Thu Sep 27 14:20:04 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 27 14:20:09 2007 Subject: [Histonet] Dermpath ??? In-Reply-To: Message-ID: <463691.73952.qm@web61216.mail.yahoo.com> This "dermatologist thread" gets scarer after each posting! Ren? J. Barbara Lord wrote: I ask my Dermatologist and he said CLIA reg that a board certified dermatologist can read his own slides. He also said though the dermatologist should be listed as laboratory personnal. Hope this helps Barbara Lord Mohs Technician Scott & White Dermatology Dept Temple , TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Moody friends. Drama queens. Your life? Nope! - their life, your story. Play Sims Stories at Yahoo! Games. From wsimms <@t> mcintoshclinic.com Thu Sep 27 14:37:57 2007 From: wsimms <@t> mcintoshclinic.com (Wesley Simms, MD) Date: Thu Sep 27 14:37:53 2007 Subject: [Histonet] Dermpath ??? References: <463691.73952.qm@web61216.mail.yahoo.com> Message-ID: <015301c8013d$e8d0ccd0$530114ac@w3256domain.com> I'm in a multispecialty group with three dermatologists. Although they don't sign out any cases, at least one of them is as qualified as any pathologist I have ever met. He doesn't have derm path certification, but he could easily sign out 80% of his cases. Some of their training programs strongly emphasize derm path, and some don't. It's actually a much smaller leap of logic for a dermatologist to sign out skin cases on patients he/she follows on a regular basis than it is for a non-physician cytotechnologist to sign out pap smears from patients they never see. Mistakes on the skin are more easily recognized than mistakes in the uterine cervix, and the clinical correlation that dermatologists have goes a long way to arriving at the correct diagnosis. Hope I didn't scare you any more than you already were, Rene. I want you to live a long time. Wesley W. Simms, M.D. ----- Original Message ----- From: "Rene J Buesa" To: "Barbara Lord" ; Sent: Thursday, September 27, 2007 3:20 PM Subject: Re: [Histonet] Dermpath ??? > This "dermatologist thread" gets scarer after each posting! > Ren? J. > > Barbara Lord wrote: > I ask my Dermatologist and he said CLIA reg that a board certified > dermatologist can read his own slides. He also said though the > dermatologist should be listed as laboratory personnal. > Hope this helps > > Barbara Lord > Mohs Technician > Scott & White Dermatology Dept > Temple , TX > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Moody friends. Drama queens. Your life? Nope! - their life, your story. > Play Sims Stories at Yahoo! Games. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From dellav <@t> musc.edu Thu Sep 27 17:47:34 2007 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Thu Sep 27 17:48:41 2007 Subject: [Histonet] Dermpath ??? In-Reply-To: References: Message-ID: <7F6B678A32B0564196138E6B3101996AC48539@EVS1.clinlan.local> Barbara, For my own benefit, if what you've indicated is true, why does the board certification for dermatopathology even exist ? I guess I'm asking what a dermatopathologist can do that a dermatologist cannot. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Lord Sent: Thursday, September 27, 2007 3:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dermpath ??? I ask my Dermatologist and he said CLIA reg that a board certified dermatologist can read his own slides. He also said though the dermatologist should be listed as laboratory personnal. Hope this helps Barbara Lord Mohs Technician Scott & White Dermatology Dept Temple , TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Thu Sep 27 19:22:08 2007 From: tkngflght <@t> yahoo.com (Cheryl R. Kerry) Date: Thu Sep 27 19:22:00 2007 Subject: [Histonet] Help--looking for Cytotechs In-Reply-To: <463691.73952.qm@web61216.mail.yahoo.com> Message-ID: <001001c80165$9bcdce60$6401a8c0@CHERYLSLAPTOP> Hi--HELP! We need a Cytologist traveler for a month-long assignment, and up to four more for permanent jobs. Thin-prep: gyn and non-gyn. I don't usually travel Cytotechs-but this is a good lab in need of some help--I know the manager and she runs a happy camp. Do you know anyone or the address for the Cytonet so I can ask there?? Cheryl Cheryl R. Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab, one great tech at a time. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone From tkngflght <@t> yahoo.com Thu Sep 27 19:22:08 2007 From: tkngflght <@t> yahoo.com (Cheryl R. Kerry) Date: Thu Sep 27 19:22:04 2007 Subject: [Histonet] Help--looking for Cytotechs In-Reply-To: <463691.73952.qm@web61216.mail.yahoo.com> Message-ID: <00b401c80165$9bd03f60$6401a8c0@CHERYLSLAPTOP> Hi--HELP! We need a Cytologist traveler for a month-long assignment, and up to four more for permanent jobs. Thin-prep: gyn and non-gyn. I don't usually travel Cytotechs-but this is a good lab in need of some help--I know the manager and she runs a happy camp. Do you know anyone or the address for the Cytonet so I can ask there?? Cheryl Cheryl R. Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab, one great tech at a time. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone From anthony <@t> histotechexchange.com Thu Sep 27 22:15:35 2007 From: anthony <@t> histotechexchange.com (anthony@histotechexchange.com) Date: Thu Sep 27 22:16:03 2007 Subject: [Histonet] St. Louis Histotech Positions Available Message-ID: <1342.65.196.168.131.1190949358.squirrel@host4.wfdns.com> Position 1: Temporary or temp-to-perm histotech position in reference lab in a beautiful suburb of St. Louis, Missouri. Grossing experience required. Must be eligible under CLIA 88 to gross. Great pay plus expenses. Position 2: Cutting, embedding and staining experience required for reference lab in a lively suburb of St. Louis, Missouri. Great pay plus expenses on a temp to temp-to-perm basis. From RSRICHMOND <@t> aol.com Thu Sep 27 23:04:52 2007 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Thu Sep 27 23:05:00 2007 Subject: [Histonet] Re: mycobacterium tuberculosis and fixation Message-ID: Vinnie Della Speranza's article about the survival of Mycobacterium tuberculosis in fixatives is at www.sakura-americas.com/histologic/pdf/02_dec.pdf Clearly he recovered what recent literature there was on the subject. This is a project that clearly needs doing - get some unfixed obviously tuberculous tissue, cut it into appropriately thin slices, and see how long after placement in neutral buffered formalin (do record the temperature!) Mycobacterium tuberculosis can still be cultured from it. Such a project could be undertaken at no very great expense in a pathology service in the developing world, and the results would be of value to all of us. An anecdote on the subject: a colleague of mine, born around 1930, was doing the surgical pathology fellowship at Memorial/Sloan Kettering in New York City around 1968, when he had a mass appear in his neck. He feared the worst, and the offending lymph node was duly excised and of course popped into formalin. As he came up from the anesthesia he realized what had probably happened, went bounding off to the pathology lab, recovered the node from the bottle of formalin, and got it cultured - and grew out Myco tuberculosis. Drug susceptibility tests were done, he got appropriate treatment, and he's still in practice in 2007. Bob Richmond Samurai Pathologist Knoxville TN From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Sep 28 02:16:34 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Sep 28 02:16:42 2007 Subject: [Histonet] Help--looking for Cytotechs Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222EDC3@wahtntex2.waht.swest.nhs.uk> Cytopathnet.org I think, google it. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Loneliness Is An island In The middle Of A sea of people --R.R. (age 11) This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Sep 28 02:21:36 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Sep 28 02:21:51 2007 Subject: [Histonet] Re: Ohh the pain (replacing formalin) Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222EDC4@wahtntex2.waht.swest.nhs.uk> 'Technicians' are trained to deal with noxious chemicals in methods to limit exposure. Untrained Staff are not 'technicians' and ought not mess with noxious chemicals. So the trick is only employ trained 'technicians' to deal with noxious chemicals and you ought not to have problems. Oh yes do make sure that they are properly trained and have the correct safety apparatus. Wonder if they'll take the semtex out of explosives just in case some of the military accidentally set it off? Oops, yes I forgot, they are trained to use it!! Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Loneliness Is An island In The middle Of A sea of people --R.R. (age 11) This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Sep 28 02:24:19 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Sep 28 02:24:27 2007 Subject: [Histonet] Re: Ohh the pain (replacing formalin) Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222EDC5@wahtntex2.waht.swest.nhs.uk> It isn't really about decreasing formalin contact or staff safety. It is about being labeled as a "green lab". Douglas D. Deltour HT(ASCP) Is that the colour of your unfixed tissue? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From pundir.arvind <@t> gmail.com Fri Sep 28 03:26:25 2007 From: pundir.arvind <@t> gmail.com (arvind) Date: Fri Sep 28 03:26:31 2007 Subject: [Histonet] sucrose cryoprotection degradation Message-ID: i have a question that if we keep the tissue in 30% sucrose for more than month will there be any problem in further proceessing the tissue for IHC *Arvind Singh Pundir* National Brain Research Centre Manesar, Gurgaon- Haryana- INDIA From pundir.arvind <@t> gmail.com Fri Sep 28 03:30:06 2007 From: pundir.arvind <@t> gmail.com (arvind) Date: Fri Sep 28 03:30:15 2007 Subject: [Histonet] hydrogen peroxide quenching- shelf life Message-ID: is there any shelf life for H2O2 so that it can be conformed before quenching unless its too late how can we know the purity of it *Arvind Singh Pundir* National Brain Research Centre Manesar, Gurgaon- Haryana- INDIA From AWeiss <@t> shorememorial.org Fri Sep 28 06:24:25 2007 From: AWeiss <@t> shorememorial.org (AWeiss@shorememorial.org) Date: Fri Sep 28 06:24:34 2007 Subject: [Histonet] Immuno's on Thin Prep specimens Message-ID: Is anyone doing IHC's on cytology specimens? If you are can you please send staining and fixation protocol. Also, what do you use for your positive and negative controls? Thank you Andrea J Weiss BST CT (ASCP) Cytotechnologist 609 653 3577 Ext 4907 aweiss@shorememorial.org This transmittal from Shore Memorial Health System is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review or use, including disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender and destroy all copies of the transmittal. From rjbuesa <@t> yahoo.com Fri Sep 28 07:42:52 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Sep 28 07:42:58 2007 Subject: [Histonet] hydrogen peroxide quenching- shelf life In-Reply-To: Message-ID: <666894.33860.qm@web61212.mail.yahoo.com> Each bottle of H2O2, either 30% or 3% has an expiration date to comply with. Ren? J. arvind wrote: is there any shelf life for H2O2 so that it can be conformed before quenching unless its too late how can we know the purity of it *Arvind Singh Pundir* National Brain Research Centre Manesar, Gurgaon- Haryana- INDIA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. From rjbuesa <@t> yahoo.com Fri Sep 28 07:49:17 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Sep 28 07:50:31 2007 Subject: [Histonet] Immuno's on Thin Prep specimens In-Reply-To: Message-ID: <182559.9008.qm@web61221.mail.yahoo.com> If using thin preps for IHC, you do NOT need to do HIER because they are acetone/air dried fixed, not formalin fixed. The rest of the IHC protocol is the same as for a FFPE tissue section. Your positive control refers to the epitope, so your regular positive control from a tissue section can be used, BUT you negative control has to be a thin prep from the same case simultaneously prepared. Ren? J. AWeiss@shorememorial.org wrote: Is anyone doing IHC's on cytology specimens? If you are can you please send staining and fixation protocol. Also, what do you use for your positive and negative controls? Thank you Andrea J Weiss BST CT (ASCP) Cytotechnologist 609 653 3577 Ext 4907 aweiss@shorememorial.org This transmittal from Shore Memorial Health System is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review or use, including disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender and destroy all copies of the transmittal. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Need a vacation? Get great deals to amazing places on Yahoo! Travel. From LSebree <@t> uwhealth.org Fri Sep 28 08:16:43 2007 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Fri Sep 28 08:16:48 2007 Subject: [Histonet] Immuno's on Thin Prep specimens In-Reply-To: <182559.9008.qm@web61221.mail.yahoo.com> Message-ID: I disagree Rene in terms of using a paraffin section as a positive control. Your two specimens (thin prep & + control) are not prepared the same. In order to get your + control to stain , you may have to employ HIER which as you state you would not do with the thin prep. That pretreatment of the + control makes it even "more different" than your patient unknown. I recently stuck my neck out at work for this very same issue. I refused to be part of running a cytospin with a FFPE staining protocol and + control. Our lab used to run cytospin IHC protocols and many times had to adjust the FFPE protocols to work for the cytopsins. At that time we used frozen section tissue controls for our positives for lack of a better alternative. These came closer to the patient specimen than FFPE controls and actually stained identically to the cytospins. Just my experience. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, September 28, 2007 7:49 AM To: AWeiss@shorememorial.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Immuno's on Thin Prep specimens If using thin preps for IHC, you do NOT need to do HIER because they are acetone/air dried fixed, not formalin fixed. The rest of the IHC protocol is the same as for a FFPE tissue section. Your positive control refers to the epitope, so your regular positive control from a tissue section can be used, BUT you negative control has to be a thin prep from the same case simultaneously prepared. Ren? J. AWeiss@shorememorial.org wrote: Is anyone doing IHC's on cytology specimens? If you are can you please send staining and fixation protocol. Also, what do you use for your positive and negative controls? Thank you Andrea J Weiss BST CT (ASCP) Cytotechnologist 609 653 3577 Ext 4907 aweiss@shorememorial.org This transmittal from Shore Memorial Health System is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review or use, including disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender and destroy all copies of the transmittal. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Need a vacation? Get great deals to amazing places on Yahoo! Travel. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kimtournear <@t> yahoo.com Fri Sep 28 08:38:17 2007 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Fri Sep 28 08:39:32 2007 Subject: [Histonet] RE: Dermatopathology question.... Message-ID: <19495.57825.qm@web50612.mail.re2.yahoo.com> Happy Friday everyone ...... Thanks to everyone for their input regarding my dermatopathology question....you where a great help... Kim Tournear, HT (ASCP), QIHC ( ASCP) Specialists in Dermatology Histology/Mohs Supervisor Tucson, AZ --------------------------------- Be a better Globetrotter. Get better travel answers from someone who knows. Yahoo! Answers - Check it out. From rjbuesa <@t> yahoo.com Fri Sep 28 08:43:53 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Sep 28 08:43:58 2007 Subject: [Histonet] Immuno's on Thin Prep specimens In-Reply-To: Message-ID: <105033.55144.qm@web61212.mail.yahoo.com> Linda: Not to start a controversy, just to discuss the issue: 1- antibodies are designed to reveal the presence of a given epitope; 2- such epitopes are the same regardless of the processing protocol or the tissues they are in, either smears, thin-preps or tissues, they just vary in concentration and distribution among the cells; 3- HIER is designed to "restore" the epitope reactive characteristics after being cross-linked by the paraffin to its "original" or "natural" condition; 4-once the epitope has been restored it is equivalent to not having ever being altered and will react identically whenever it is present. IF your usual protocol to detect the epitope in your positive tissue control is not altered from its usual way and renders the same reaction intensitywise on the same target cells, it should also react in the thin prep treated identically. I realize that since you "recently stuck your head" on this issue and refused to do something like I am advocating, it will be rather difficult for you to analyze the precedent paragraphs passionlessly. but give a chance to reasoning. On the other hand, I have done hundreds of IHC on thin preps and always used FFPE tissue sections as positive controls. The positive controls always behaved as expected and the thin preps, treated simultaneously rendered positive or negative results, according with their specific nature. The thing that you have to be consistent with is using another identical thin prep as negaive control. Ren? J. "Sebree Linda A." wrote: I disagree Rene in terms of using a paraffin section as a positive control. Your two specimens (thin prep & + control) are not prepared the same. In order to get your + control to stain , you may have to employ HIER which as you state you would not do with the thin prep. That pretreatment of the + control makes it even "more different" than your patient unknown. I recently stuck my neck out at work for this very same issue. I refused to be part of running a cytospin with a FFPE staining protocol and + control. Our lab used to run cytospin IHC protocols and many times had to adjust the FFPE protocols to work for the cytopsins. At that time we used frozen section tissue controls for our positives for lack of a better alternative. These came closer to the patient specimen than FFPE controls and actually stained identically to the cytospins. Just my experience. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, September 28, 2007 7:49 AM To: AWeiss@shorememorial.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Immuno's on Thin Prep specimens If using thin preps for IHC, you do NOT need to do HIER because they are acetone/air dried fixed, not formalin fixed. The rest of the IHC protocol is the same as for a FFPE tissue section. Your positive control refers to the epitope, so your regular positive control from a tissue section can be used, BUT you negative control has to be a thin prep from the same case simultaneously prepared. Ren? J. AWeiss@shorememorial.org wrote: Is anyone doing IHC's on cytology specimens? If you are can you please send staining and fixation protocol. Also, what do you use for your positive and negative controls? Thank you Andrea J Weiss BST CT (ASCP) Cytotechnologist 609 653 3577 Ext 4907 aweiss@shorememorial.org This transmittal from Shore Memorial Health System is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review or use, including disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender and destroy all copies of the transmittal. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Need a vacation? Get great deals to amazing places on Yahoo! Travel. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Tonight's top picks. What will you watch tonight? Preview the hottest shows on Yahoo! TV. From LeslieS <@t> vetmed.ufl.edu Fri Sep 28 09:01:49 2007 From: LeslieS <@t> vetmed.ufl.edu (Shawn Leslie) Date: Fri Sep 28 09:02:12 2007 Subject: [Histonet] Immuno's on Thin Prep specimens In-Reply-To: <105033.55144.qm@web61212.mail.yahoo.com> References: <105033.55144.qm@web61212.mail.yahoo.com> Message-ID: <46FCD10D.CC0E.007B.0@vetmed.ufl.edu> Just a correction Rene. You stated "HIER is designed to "restore" the epitope reactive characteristics after being cross-linked by the paraffin ". Actually HIER was introduced to demask the epitope after being cross-linked (methylene bridges) by the formalin not the paraffin.... Just wanted make that correction Shawn Leslie Scientific Research Manager Anatomic Pathology University of Florida School of Veterinary Medicine 352-392-2235 ext 4555 >>> Rene J Buesa 9/28/2007 9:43 AM >>> Linda: Not to start a controversy, just to discuss the issue: 1- antibodies are designed to reveal the presence of a given epitope; 2- such epitopes are the same regardless of the processing protocol or the tissues they are in, either smears, thin-preps or tissues, they just vary in concentration and distribution among the cells; 3- HIER is designed to "restore" the epitope reactive characteristics after being cross-linked by the paraffin to its "original" or "natural" condition; 4-once the epitope has been restored it is equivalent to not having ever being altered and will react identically whenever it is present. IF your usual protocol to detect the epitope in your positive tissue control is not altered from its usual way and renders the same reaction intensitywise on the same target cells, it should also react in the thin prep treated identically. I realize that since you "recently stuck your head" on this issue and refused to do something like I am advocating, it will be rather difficult for you to analyze the precedent paragraphs passionlessly. but give a chance to reasoning. On the other hand, I have done hundreds of IHC on thin preps and always used FFPE tissue sections as positive controls. The positive controls always behaved as expected and the thin preps, treated simultaneously rendered positive or negative results, according with their specific nature. The thing that you have to be consistent with is using another identical thin prep as negaive control. Ren? J. "Sebree Linda A." wrote: I disagree Rene in terms of using a paraffin section as a positive control. Your two specimens (thin prep & + control) are not prepared the same. In order to get your + control to stain , you may have to employ HIER which as you state you would not do with the thin prep. That pretreatment of the + control makes it even "more different" than your patient unknown. I recently stuck my neck out at work for this very same issue. I refused to be part of running a cytospin with a FFPE staining protocol and + control. Our lab used to run cytospin IHC protocols and many times had to adjust the FFPE protocols to work for the cytopsins. At that time we used frozen section tissue controls for our positives for lack of a better alternative. These came closer to the patient specimen than FFPE controls and actually stained identically to the cytospins. Just my experience. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, September 28, 2007 7:49 AM To: AWeiss@shorememorial.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Immuno's on Thin Prep specimens If using thin preps for IHC, you do NOT need to do HIER because they are acetone/air dried fixed, not formalin fixed. The rest of the IHC protocol is the same as for a FFPE tissue section. Your positive control refers to the epitope, so your regular positive control from a tissue section can be used, BUT you negative control has to be a thin prep from the same case simultaneously prepared. Ren? J. AWeiss@shorememorial.org wrote: Is anyone doing IHC's on cytology specimens? If you are can you please send staining and fixation protocol. Also, what do you use for your positive and negative controls? Thank you Andrea J Weiss BST CT (ASCP) Cytotechnologist 609 653 3577 Ext 4907 aweiss@shorememorial.org This transmittal from Shore Memorial Health System is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review or use, including disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender and destroy all copies of the transmittal. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Need a vacation? Get great deals to amazing places on Yahoo! Travel. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Tonight's top picks. What will you watch tonight? Preview the hottest shows on Yahoo! TV. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TownsendD <@t> childrensdayton.org Fri Sep 28 09:08:24 2007 From: TownsendD <@t> childrensdayton.org (Dolores Townsend) Date: Fri Sep 28 09:08:57 2007 Subject: [Histonet] OT Re: Hydrogen Peroxide quenching Message-ID: It starts with a cage containing five monkeys. Inside the cage hangs a banana on a string and under it a set of stairs. Before long, a monkey will go to the stairs and start to climb towards the banana. As soon as he touches the stairs, spray all of the other monkeys with cold water. After a while, another monkey makes an attempt with the same result all the other monkeys are sprayed with cold water. Pretty soon, when another monkey tries to climb the stairs, the other monkeys will try to prevent it. Now, put away the cold water. Remove one monkey from the cage and replace it with a new one. The new monkey sees the banana and wants to climb the stairs. To his surprise and horror, all of the other monkeys attack him. After another attempt and attack, he knows that if he tries to climb the stairs, he will be assaulted.Next, remove another of the original five monkeys and replace it with a new one. The newcomer goes to the stairs and is attacked. The previous newcomer takes part in the punishment with enthusiasm! Likewise, replace a third original monkey with a new one, then a fourth, then the fifth. Every time the newest monkey takes to the stairs, he is attacked. Most of the monkeys that are beating him have no idea why they were not permitted to climb the stairs or why they are participating in the beating of the newest monkey.After replacing all the original monkeys, none of the remaining monkeys have ever been sprayed with cold water. Nevertheless, no monkey ever again approaches the stairs to try for the banana. Why not? Because as far as they know that?s the way it?s always been done around here.And that, my friends, is how a company policy begins From tissuearray <@t> hotmail.com Fri Sep 28 09:08:53 2007 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Fri Sep 28 09:09:01 2007 Subject: [Histonet] transfering tissue to another slide? Message-ID: Hey, Has anyone ever taken tissue off one slide and transfering it to another? I think I remember someone mentioning it in the past. Anyone know anything? Thanks, Thom for information on instructing TMA go to: www.arrayworkshop.com New array instrument: www.arraymold.com _________________________________________________________________ Invite your mail contacts to join your friends list with Windows Live Spaces. It's easy! http://spaces.live.com/spacesapi.aspx?wx_action=create&wx_url=/friends.aspx&mkt=en-us From JMcCormick <@t> schosp.org Fri Sep 28 09:11:53 2007 From: JMcCormick <@t> schosp.org (McCormick, James) Date: Fri Sep 28 09:13:06 2007 Subject: [Histonet] Dermpath ??? In-Reply-To: <7F6B678A32B0564196138E6B3101996AC48539@EVS1.clinlan.local> References: <7F6B678A32B0564196138E6B3101996AC48539@EVS1.clinlan.local> Message-ID: <913FAC2B773C19488E26AE6572180FA5082DBF95@exch01.schosp.org> Barbara, Vinnie et al, Concerning medical specialists interpreting slides and dermatopathology in particular.... "in the beginning" there were no histopathologists. The specialty groups in clinical practice supported their own gross and microscopic study and research. Hematologists interpret and diagnose blood and bone marrow preparations, some university Ob/Gyne departments have separate divisions of gross and microscopic pathology. Many dermatologists are deeply trained and experienced in "reading" the slides as well as the surface skin. Large groups of dermatologists and some urologists have installed histotechnology labs and share their work with a "group" pathologist of the general or special skill set and experience. The state licenses to practice medicine does not restrict against the study of slides. Quality standards of hospitals require the best in all areas of clinical and laboratory medicine. Civil law only requires that a "qualified" director be in charge of the lab. What specialty physicians do in their private offices does not restrict them to NOT use the microscope.....It is the training and experience to achieve quality and excellence that promotes the "sub specialization" of pathology and other laboratory associated practices. In Europe the Gynecologist is in charge of cytopathology. This is just my "two cents" from some years in the practice of pathology...and with a special leaning to histopathology process and quality. J.B.McCormick, M.D. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Thursday, September 27, 2007 5:48 PM To: Barbara Lord; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dermpath ??? Barbara, For my own benefit, if what you've indicated is true, why does the board certification for dermatopathology even exist ? I guess I'm asking what a dermatopathologist can do that a dermatologist cannot. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Lord Sent: Thursday, September 27, 2007 3:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dermpath ??? I ask my Dermatologist and he said CLIA reg that a board certified dermatologist can read his own slides. He also said though the dermatologist should be listed as laboratory personnal. Hope this helps Barbara Lord Mohs Technician Scott & White Dermatology Dept Temple , TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. From rjbuesa <@t> yahoo.com Fri Sep 28 09:29:34 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Sep 28 09:29:39 2007 Subject: [Histonet] transfering tissue to another slide? In-Reply-To: Message-ID: <6084.64741.qm@web61216.mail.yahoo.com> Only very small areas to be used for TEM attached to the tip of the epoxi cone when there was not fresh available tissue. Whole sections will tear appart completely. Ren? J. Thom Jensen wrote: Hey, Has anyone ever taken tissue off one slide and transfering it to another? I think I remember someone mentioning it in the past. Anyone know anything? Thanks, Thom for information on instructing TMA go to: www.arrayworkshop.com New array instrument: www.arraymold.com _________________________________________________________________ Invite your mail contacts to join your friends list with Windows Live Spaces. It's easy! http://spaces.live.com/spacesapi.aspx?wx_action=create&wx_url=/friends.aspx&mkt=en-us_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Catch up on fall's hot new shows on Yahoo! TV. Watch previews, get listings, and more! From Terry.Marshall <@t> rothgen.nhs.uk Fri Sep 28 09:30:17 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 28 09:30:22 2007 Subject: [Histonet] transfering tissue to another slide? Message-ID: <407F05A128805F4C879A33DBA32E618E018950A5@TRFT-EX01.xRothGen.nhs.uk> Kemlo, We had a tube of "stuff" in Stoke which worked reasonably well. I have not come across it since. Remember the name? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thom Jensen Sent: 28 September 2007 15:09 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] transfering tissue to another slide? Hey, Has anyone ever taken tissue off one slide and transfering it to another? I think I remember someone mentioning it in the past. Anyone know anything? Thanks, Thom for information on instructing TMA go to: www.arrayworkshop.com New array instrument: www.arraymold.com _________________________________________________________________ Invite your mail contacts to join your friends list with Windows Live Spaces. It's easy! http://spaces.live.com/spacesapi.aspx?wx_action=create&wx_url=/friends.a spx&mkt=en-us_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bwhitaker <@t> brownpathology.com Fri Sep 28 09:35:01 2007 From: bwhitaker <@t> brownpathology.com (Bonnie Whitaker) Date: Fri Sep 28 09:31:53 2007 Subject: [Histonet] hydrogen peroxide quenching- shelf life In-Reply-To: <666894.33860.qm@web61212.mail.yahoo.com> Message-ID: <005f01c801dc$c10fcc10$3601a8c0@brownpathology.net> That is true, but in my experience, after opening the bottle it tends to degrade at a rate that makes the expiration date useless. (That is unless you are using it up at a really fast rate.) You could check it periodically with a really bloody specimen that goes in the H2O2 block, per your protocol, and then add DAB per your protocol. If it turns brown, it's time for a new bottle. Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, September 28, 2007 7:43 AM To: arvind; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] hydrogen peroxide quenching- shelf life Each bottle of H2O2, either 30% or 3% has an expiration date to comply with. Ren? J. arvind wrote: is there any shelf life for H2O2 so that it can be conformed before quenching unless its too late how can we know the purity of it *Arvind Singh Pundir* National Brain Research Centre Manesar, Gurgaon- Haryana- INDIA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Sep 28 09:32:48 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Sep 28 09:32:55 2007 Subject: Fwd: RE: [Histonet] Immuno's on Thin Prep specimens Message-ID: <442179.70177.qm@web61219.mail.yahoo.com> Rene J Buesa wrote: Date: Fri, 28 Sep 2007 07:21:26 -0700 (PDT) From: Rene J Buesa Subject: RE: [Histonet] Immuno's on Thin Prep specimens To: Shawn Leslie Leslie: My ABSOLUTE involuntary mistake. You are right, formalin crosslinkage is the one "targeted" by HIER, to undo what formalin did to the epitope reactivity. But let me tell you, there is something also HIER is intended to: in 1980 JB Matthews points out in "Influence of clearing agent on immunohistochemical staining of paraffin-emebedde tissue" that the omnipresent XYLENE causes: "Many laboratories use xylene as their routine clearing agent, which has been shown to have an extracting quality on cell cytoplasm as seen ultrastructuraly and to decrease immunoreactivity" (sic.). This author further states: "The results indicate that xylene processing is deleterious to the immunoreactivity of tissue antigens and that the choice of clearing agent may be an important factor determining the ability to stain non-trypsinised sections of formalin-fixed, paraffin-embedde tissue". This author was working in a pre-HIER period and used trypsine instead. So, BOTH formalin AND xylene reduce the epitope reactivity. When I totally eliminated xylene from my tissue processing protocols I noticed an intensity increase in the IHC reactions of the order of 1+ step in intensity. This author did this research at the Immunology Lab., Dept. of Oral Pathology, The Dental School, St. Chad's Queensway, Birminham B4 6NN, UK Thank you for pointing out my "slip of the mind/fingers". Ren? J. Shawn Leslie wrote: Just a correction Rene. You stated "HIER is designed to "restore" the epitope reactive characteristics after being cross-linked by the paraffin ". Actually HIER was introduced to demask the epitope after being cross-linked (methylene bridges) by the formalin not the paraffin.... Just wanted make that correction Shawn Leslie Scientific Research Manager Anatomic Pathology University of Florida School of Veterinary Medicine 352-392-2235 ext 4555 >>> Rene J Buesa 9/28/2007 9:43 AM >>> Linda: Not to start a controversy, just to discuss the issue: 1- antibodies are designed to reveal the presence of a given epitope; 2- such epitopes are the same regardless of the processing protocol or the tissues they are in, either smears, thin-preps or tissues, they just vary in concentration and distribution among the cells; 3- HIER is designed to "restore" the epitope reactive characteristics after being cross-linked by the paraffin to its "original" or "natural" condition; 4-once the epitope has been restored it is equivalent to not having ever being altered and will react identically whenever it is present. IF your usual protocol to detect the epitope in your positive tissue control is not altered from its usual way and renders the same reaction intensitywise on the same target cells, it should also react in the thin prep treated identically. I realize that since you "recently stuck your head" on this issue and refused to do something like I am advocating, it will be rather difficult for you to analyze the precedent paragraphs passionlessly. but give a chance to reasoning. On the other hand, I have done hundreds of IHC on thin preps and always used FFPE tissue sections as positive controls. The positive controls always behaved as expected and the thin preps, treated simultaneously rendered positive or negative results, according with their specific nature. The thing that you have to be consistent with is using another identical thin prep as negaive control. Ren? J. "Sebree Linda A." wrote: I disagree Rene in terms of using a paraffin section as a positive control. Your two specimens (thin prep & + control) are not prepared the same. In order to get your + control to stain , you may have to employ HIER which as you state you would not do with the thin prep. That pretreatment of the + control makes it even "more different" than your patient unknown. I recently stuck my neck out at work for this very same issue. I refused to be part of running a cytospin with a FFPE staining protocol and + control. Our lab used to run cytospin IHC protocols and many times had to adjust the FFPE protocols to work for the cytopsins. At that time we used frozen section tissue controls for our positives for lack of a better alternative. These came closer to the patient specimen than FFPE controls and actually stained identically to the cytospins. Just my experience. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, September 28, 2007 7:49 AM To: AWeiss@shorememorial.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Immuno's on Thin Prep specimens If using thin preps for IHC, you do NOT need to do HIER because they are acetone/air dried fixed, not formalin fixed. The rest of the IHC protocol is the same as for a FFPE tissue section. Your positive control refers to the epitope, so your regular positive control from a tissue section can be used, BUT you negative control has to be a thin prep from the same case simultaneously prepared. Ren? J. AWeiss@shorememorial.org wrote: Is anyone doing IHC's on cytology specimens? If you are can you please send staining and fixation protocol. Also, what do you use for your positive and negative controls? Thank you Andrea J Weiss BST CT (ASCP) Cytotechnologist 609 653 3577 Ext 4907 aweiss@shorememorial.org This transmittal from Shore Memorial Health System is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review or use, including disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender and destroy all copies of the transmittal. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Need a vacation? Get great deals to amazing places on Yahoo! Travel. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Tonight's top picks. What will you watch tonight? Preview the hottest shows on Yahoo! TV. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Fussy? Opinionated? Impossible to please? Perfect. Join Yahoo!'s user panel and lay it on us. --------------------------------- Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: mail, news, photos & more. From RSRICHMOND <@t> aol.com Fri Sep 28 09:52:41 2007 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Fri Sep 28 09:52:52 2007 Subject: [Histonet] Re: Dermpath ??? Message-ID: This thread has some issues that might be worth untangling. Dermatopathology is a subspecialty certification. Pathologists and dermatologists who have their respective basic board certifications are eligible to sit the exam for the dermatopathology certification. A pathologist with anatomic pathology boards is qualified to sign out dermatopathology cases. So is a dermatologist with dermatopathology boards. (I'm not familiar with the various issues concerning dermatologists without derm path certification.) It's a great deal easier to do dermatopathology for a dermatologist than it is to do it for a primary care practitioner who's on the loose with a biopsy punch. The dermatologist can give me a list of possible diagnoses which I then, if I'm completely at sea, I can crank through with a good derm path textbook such as Weedon. The GP who sends me a history of "rash" and can offer no more information when I call him - now that case needs a dermatopathologist to sign it out! Mohs surgeons read their own frozen sections as they work, and a pathologist is little help to them. Most Mohs surgeons unload anything else on a pathologist! A Mohs surgeon should be highly qualified to read his own frozen sections. With this important exception - in general it's not a good idea for clinicians to read slides on their own patients. Bob Richmond Samurai Pathologist Knoxville TN From Pat.Bell <@t> UCHSC.edu Fri Sep 28 10:01:42 2007 From: Pat.Bell <@t> UCHSC.edu (Pat.Bell@UCHSC.edu) Date: Fri Sep 28 10:03:35 2007 Subject: [Histonet] transfering tissue to another slide? In-Reply-To: Message-ID: <71FCC52823941D49A4BC39B48C6E3428F3BE13@java.uchsc.edu> The procedure to do it is in the AFIP Laboratory Methods in Histotechnology. I have tried it and it works great. Pat Bell University of Co. Health Sciences Center Aurora, Colorado 80045 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thom Jensen Sent: Friday, September 28, 2007 8:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] transfering tissue to another slide? Hey, Has anyone ever taken tissue off one slide and transfering it to another? I think I remember someone mentioning it in the past. Anyone know anything? Thanks, Thom for information on instructing TMA go to: www.arrayworkshop.com New array instrument: www.arraymold.com _________________________________________________________________ Invite your mail contacts to join your friends list with Windows Live Spaces. It's easy! http://spaces.live.com/spacesapi.aspx?wx_action=create&wx_url=/friends.a spx&mkt=en-us_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LeslieS <@t> vetmed.ufl.edu Fri Sep 28 10:02:01 2007 From: LeslieS <@t> vetmed.ufl.edu (Shawn Leslie) Date: Fri Sep 28 10:03:39 2007 Subject: [Histonet] transfering tissue to another slide? In-Reply-To: References: Message-ID: <46FCDF2C.CC0E.007B.0@vetmed.ufl.edu> We once used something called Diatex from Dow Corning.The Address is on page 69 of the A.F.I.P. manual.I'm not sure if they even sell anymore. That was a while ago.... Shawn Leslie Scientific Research Manager Anatomic Pathology University of Florida School of Veterinary Medicine 352-392-2235 ext 4555 >>> Thom Jensen 9/28/2007 10:08 AM >>> Hey, Has anyone ever taken tissue off one slide and transfering it to another? I think I remember someone mentioning it in the past. Anyone know anything? Thanks, Thom for information on instructing TMA go to: www.arrayworkshop.com New array instrument: www.arraymold.com _________________________________________________________________ Invite your mail contacts to join your friends list with Windows Live Spaces. It's easy! http://spaces.live.com/spacesapi.aspx?wx_action=create&wx_url=/friends.aspx&mkt=en-us_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Fri Sep 28 10:04:27 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 28 10:04:37 2007 Subject: [Histonet] Re: Dermpath ??? Message-ID: <407F05A128805F4C879A33DBA32E618E018950A6@TRFT-EX01.xRothGen.nhs.uk> "With this important exception - in general it's not a good idea for clinicians to read slides on their own patients." Try telling that to the dermatologists who do their own. One claimed to earn twice as much as I earn, full time, from doing 2 sessions a week. Nice work if you can get it. The whole point of dermatologists doing skin pathology, is that they just simply have a better idea of what they are doing. Remember, there are significantly more named diseases in the skin than everywhere else put together. I do however think they are in great danger of agreeing with themselves, if you see what I mean:-) Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: 28 September 2007 15:53 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Dermpath ??? This thread has some issues that might be worth untangling. Dermatopathology is a subspecialty certification. Pathologists and dermatologists who have their respective basic board certifications are eligible to sit the exam for the dermatopathology certification. A pathologist with anatomic pathology boards is qualified to sign out dermatopathology cases. So is a dermatologist with dermatopathology boards. (I'm not familiar with the various issues concerning dermatologists without derm path certification.) It's a great deal easier to do dermatopathology for a dermatologist than it is to do it for a primary care practitioner who's on the loose with a biopsy punch. The dermatologist can give me a list of possible diagnoses which I then, if I'm completely at sea, I can crank through with a good derm path textbook such as Weedon. The GP who sends me a history of "rash" and can offer no more information when I call him - now that case needs a dermatopathologist to sign it out! Mohs surgeons read their own frozen sections as they work, and a pathologist is little help to them. Most Mohs surgeons unload anything else on a pathologist! A Mohs surgeon should be highly qualified to read his own frozen sections. With this important exception - in general it's not a good idea for clinicians to read slides on their own patients. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From slappycraw <@t> yahoo.com Fri Sep 28 10:14:19 2007 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Fri Sep 28 10:14:23 2007 Subject: [Histonet] transfering tissue to another slide? In-Reply-To: Message-ID: <767112.7999.qm@web53611.mail.re2.yahoo.com> Yes, here it is. 1. Take the coverslip off the slide 2. If it is stained and you want to restain it, then destain it then run it down to water either way. 3. Harleco makes a mountant called Krystalon and you need to dry the slide from the water and apply the krystalon so it covers the entire tissue surrounding it to form a mound. 4. Put the slide flat into a 60-90 degree oven overnight. 5. Next day take the slide out and using a copeland jar, put 50ml of dist water in it and microwave the water until it is pretty hot. 6. Put the slide with the mountant on it into the hot water for about 10 min. 7. Take the slide out and with a needle nose forceps start to pull up the edge of the mountant slowly. You may have to put the slide back in the hot water a couple of times to do this. 8. Finally take the mountant that peeled off of the slide with the tissue attached and lay it onto a clean and dry slide, using a thick paper towel to press it down flat (be careful not to break the slide that you are pressing on) make sure the slide is on a thick paper towel as well. 9. Put the slide in the oven again overnight ( I have seen it done in 2 hours) and then run down from xylene to get the mountant off and presto, your tissue is now on another slide. Thom Jensen wrote: Hey, Has anyone ever taken tissue off one slide and transfering it to another? I think I remember someone mentioning it in the past. Anyone know anything? Thanks, Thom for information on instructing TMA go to: www.arrayworkshop.com New array instrument: www.arraymold.com _________________________________________________________________ Invite your mail contacts to join your friends list with Windows Live Spaces. It's easy! http://spaces.live.com/spacesapi.aspx?wx_action=create&wx_url=/friends.aspx&mkt=en-us_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Larry A. Woody Amgen Seattle, Wa. --------------------------------- Tonight's top picks. What will you watch tonight? Preview the hottest shows on Yahoo! TV. From oshel1pe <@t> cmich.edu Fri Sep 28 10:29:48 2007 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Fri Sep 28 10:29:58 2007 Subject: [Histonet] Re: Dermpath ??? In-Reply-To: <407F05A128805F4C879A33DBA32E618E018950A6@TRFT-EX01.xRothGen.nhs.uk> References: <407F05A128805F4C879A33DBA32E618E018950A6@TRFT-EX01.xRothGen.nhs.uk> Message-ID: "I wouldn't have seen it if I hadn't believed it." Phil > "With this important exception - in general it's not a good idea for >clinicians to read slides on their own patients." > >Try telling that to the dermatologists who do their own. >One claimed to earn twice as much as I earn, full time, from doing 2 >sessions a week. >Nice work if you can get it. > >The whole point of dermatologists doing skin pathology, is that they >just simply have a better idea of what they are doing. Remember, there >are significantly more named diseases in the skin than everywhere else >put together. > >I do however think they are in great danger of agreeing with themselves, >if you see what I mean:-) > >Terry > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert >Richmond >Sent: 28 September 2007 15:53 >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Re: Dermpath ??? > >This thread has some issues that might be worth untangling. > >Dermatopathology is a subspecialty certification. Pathologists and >dermatologists who have their respective basic board certifications are >eligible to sit the exam for the dermatopathology certification. > >A pathologist with anatomic pathology boards is qualified to sign out >dermatopathology cases. So is a dermatologist with dermatopathology >boards. (I'm not familiar with the various issues concerning >dermatologists without derm path certification.) > >It's a great deal easier to do dermatopathology for a dermatologist than >it is to do it for a primary care practitioner who's on the loose with a >biopsy punch. The dermatologist can give me a list of possible diagnoses >which I then, if I'm completely at sea, I can crank through with a good >derm path textbook such as Weedon. The GP who sends me a history of >"rash" and can offer no more information when I call him - now that case >needs a dermatopathologist to sign it out! > >Mohs surgeons read their own frozen sections as they work, and a >pathologist is little help to them. Most Mohs surgeons unload anything >else on a pathologist! A Mohs surgeon should be highly qualified to read >his own frozen sections. > >With this important exception - in general it's not a good idea for >clinicians to read slides on their own patients. > >Bob Richmond >Samurai Pathologist >Knoxville TN > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rgarhart <@t> system1.net Fri Sep 28 10:35:18 2007 From: rgarhart <@t> system1.net (Robert Garhart) Date: Fri Sep 28 10:35:22 2007 Subject: [Histonet] PhD FISH Director Opportunity In-Reply-To: <000201c77d29$5ef145f0$800aa8c0@domain.local> References: <000201c77d29$5ef145f0$800aa8c0@domain.local> Message-ID: I have a client in the Greater NYC area that is looking for a PhD candidate FISH Director. Must be a strong leader and prefer that they be a local candidate to the NY/NJ area. May consider relo assistance for right candidate. Will be involved in managing the processes and matrices. Must have UroVysion experience and preference is to also have NY COQ, though if other factors are met we may be able to get by that. Any interested individuals please call Robert Garhart. 678-342-9029 or send email to rgarhart@system1.net. Please feel free to pass this along to others who may find it useful. This client is also looking to at local NJ based Cytotechs and FISH prep Techs as they continue to grow. Robert Garhart Executive Recruiter System 1 Search 678-342-9029 Office rgarhart@system1.net Website: www.system1.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan Plaza Sent: Thursday, April 12, 2007 10:38 AM To: histonet@lists.utsouthwestern.edu; ':' Subject: [Histonet] Job Opening in East Texas Full-time Histotechnologist/Histologic Technician position available at Pathology Associates of Tyler in scenic East Texas. Should be ASCP registered or registry-eligible. P.A.T. offers a highly competitive salary with an excellent benefits package. Interested applicants should forward their resume to: Pathology Associates of Tyler, 1726 South Beckham Ave., Tyler, TX 75701. Fax: (903)592-0555. Phone: (903)593-0481 or e-mail: sgibson@tylerpathology.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Fri Sep 28 10:43:11 2007 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Fri Sep 28 10:43:21 2007 Subject: [Histonet] Immuno's on Thin Prep specimens In-Reply-To: <105033.55144.qm@web61212.mail.yahoo.com> Message-ID: Rene, Let's agree to disagree on this issue. CAP guidelines state to use positive control specimens prepared the same way as the patient unknown. I don't think your procedure adheres to that. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Friday, September 28, 2007 8:44 AM To: Sebree Linda A.; AWeiss@shorememorial.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Immuno's on Thin Prep specimens Linda: Not to start a controversy, just to discuss the issue: 1- antibodies are designed to reveal the presence of a given epitope; 2- such epitopes are the same regardless of the processing protocol or the tissues they are in, either smears, thin-preps or tissues, they just vary in concentration and distribution among the cells; 3- HIER is designed to "restore" the epitope reactive characteristics after being cross-linked by the paraffin to its "original" or "natural" condition; 4-once the epitope has been restored it is equivalent to not having ever being altered and will react identically whenever it is present. IF your usual protocol to detect the epitope in your positive tissue control is not altered from its usual way and renders the same reaction intensitywise on the same target cells, it should also react in the thin prep treated identically. I realize that since you "recently stuck your head" on this issue and refused to do something like I am advocating, it will be rather difficult for you to analyze the precedent paragraphs passionlessly. but give a chance to reasoning. On the other hand, I have done hundreds of IHC on thin preps and always used FFPE tissue sections as positive controls. The positive controls always behaved as expected and the thin preps, treated simultaneously rendered positive or negative results, according with their specific nature. The thing that you have to be consistent with is using another identical thin prep as negaive control. Ren? J. "Sebree Linda A." wrote: I disagree Rene in terms of using a paraffin section as a positive control. Your two specimens (thin prep & + control) are not prepared the same. In order to get your + control to stain , you may have to employ HIER which as you state you would not do with the thin prep. That pretreatment of the + control makes it even "more different" than your patient unknown. I recently stuck my neck out at work for this very same issue. I refused to be part of running a cytospin with a FFPE staining protocol and + control. Our lab used to run cytospin IHC protocols and many times had to adjust the FFPE protocols to work for the cytopsins. At that time we used frozen section tissue controls for our positives for lack of a better alternative. These came closer to the patient specimen than FFPE controls and actually stained identically to the cytospins. Just my experience. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, September 28, 2007 7:49 AM To: AWeiss@shorememorial.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Immuno's on Thin Prep specimens If using thin preps for IHC, you do NOT need to do HIER because they are acetone/air dried fixed, not formalin fixed. The rest of the IHC protocol is the same as for a FFPE tissue section. Your positive control refers to the epitope, so your regular positive control from a tissue section can be used, BUT you negative control has to be a thin prep from the same case simultaneously prepared. Ren? J. AWeiss@shorememorial.org wrote: Is anyone doing IHC's on cytology specimens? If you are can you please send staining and fixation protocol. Also, what do you use for your positive and negative controls? Thank you Andrea J Weiss BST CT (ASCP) Cytotechnologist 609 653 3577 Ext 4907 aweiss@shorememorial.org This transmittal from Shore Memorial Health System is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review or use, including disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender and destroy all copies of the transmittal. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Need a vacation? Get great deals to amazing places on Yahoo! Travel. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Tonight's top picks. What will you watch tonight? Preview the hottest shows on Yahoo! TV. From LSebree <@t> uwhealth.org Fri Sep 28 10:45:24 2007 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Fri Sep 28 10:45:30 2007 Subject: [Histonet] transfering tissue to another slide? In-Reply-To: Message-ID: I believe the people at ProPath know how to do that. Phone: (800)258-1253, ext. 2037 Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thom Jensen Sent: Friday, September 28, 2007 9:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] transfering tissue to another slide? Hey, Has anyone ever taken tissue off one slide and transfering it to another? I think I remember someone mentioning it in the past. Anyone know anything? Thanks, Thom for information on instructing TMA go to: www.arrayworkshop.com New array instrument: www.arraymold.com _________________________________________________________________ Invite your mail contacts to join your friends list with Windows Live Spaces. It's easy! http://spaces.live.com/spacesapi.aspx?wx_action=create&wx_url=/friends.a spx&mkt=en-us_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ian.montgomery <@t> bio.gla.ac.uk Fri Sep 28 11:02:21 2007 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Fri Sep 28 11:02:29 2007 Subject: FW: [Histonet] transfering tissue to another slide? Message-ID: <003701c801e8$f4200a00$6424d182@IBLS.GLA.AC.UK> Terry, The tube of "stuff" was Durofix. Cast your mind back to when you were a boy and built Airfix models, it's the same glue. Over the years I've searched for Durofix but think it's no longer on the market. Technique. 1.) Remove the coverglass by soaking in warm xylene or equivalent. 2.) Soak in fresh clearing agent until you are sure the mounting media has been removed. 3.) Cover the section with a mixture of 6 parts butyl-acetate, 1 part Durofix. 4.) Harden in a 37C oven for ~30 minutes. 5.) Carefully, with a sharp razor blade, cut the film from around the section. 6.) Place the slide in cold water and leave until the film with the section floats off. 7.) Mount on a clean slide. 8.) Drain excess water then place in a 37C oven until perfectly dry. 9.) Wash carefully with butyl-acetate. 10) Wash thoroughly in clearing agent and mount. When Durofix was available I used the technique on numerous occasions and with a few tweeks it worked well. All you need do is find glue that bonds plastics and you're away. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, G12 8QQ. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: 28 September 2007 15:30 To: Thom Jensen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] transfering tissue to another slide? Kemlo, We had a tube of "stuff" in Stoke which worked reasonably well. I have not come across it since. Remember the name? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thom Jensen Sent: 28 September 2007 15:09 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] transfering tissue to another slide? Hey, Has anyone ever taken tissue off one slide and transfering it to another? I think I remember someone mentioning it in the past. Anyone know anything? Thanks, Thom for information on instructing TMA go to: www.arrayworkshop.com New array instrument: www.arraymold.com _________________________________________________________________ Invite your mail contacts to join your friends list with Windows Live Spaces. It's easy! http://spaces.live.com/spacesapi.aspx?wx_action=create&wx_url=/friends.a spx&mkt=en-us_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Fri Sep 28 11:09:13 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 28 11:09:18 2007 Subject: [Histonet] transfering tissue to another slide? Message-ID: The method on p. 69 of the AFIP manual is for covering some of the sections on a slide with an impervious material that prevents staining, so that you can apply two or more techniques to different sections on the same slide. It's not for removing and transferring sections. Alkaline solutions are pretty good at removing sections from slides, and it's sometimes possible (with skill and luck) to remount them. Before trying, acidify and then rinse the section in water. Sections floating in an alkaline medium will not survive attempts to remove folds and creases. John Kiernan Anatomy, UWO London, Canada --- ----- Original Message ----- From: Shawn Leslie Date: Friday, September 28, 2007 11:05 Subject: Re: [Histonet] transfering tissue to another slide? To: Thom Jensen , "histonet@lists.utsouthwestern.edu" > We once used something called Diatex from Dow Corning.The > Address is on page 69 of the A.F.I.P. manual.I'm not sure if > they even sell anymore. That was a while ago.... > > > > Shawn Leslie > Scientific Research Manager > Anatomic Pathology > University of Florida > School of Veterinary Medicine > 352-392-2235 ext 4555 > > > >>> Thom Jensen 9/28/2007 10:08 AM >>> > > Hey, > Has anyone ever taken tissue off one slide and transfering it to > another? I think I remember someone mentioning it in the past. > > Anyone know anything? > > Thanks, > Thom > > > for information on instructing TMA go to: > www.arrayworkshop.com > > New array instrument: www.arraymold.com > > > _________________________________________________________________ > Invite your mail contacts to join your friends list with Windows > Live Spaces. It's easy! > http://spaces.live.com/spacesapi.aspx?wx_action=create&wx_url=/friends.aspx&mkt=en-us_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Terry.Marshall <@t> rothgen.nhs.uk Fri Sep 28 11:13:53 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 28 11:14:01 2007 Subject: [Histonet] transfering tissue to another slide? Message-ID: <407F05A128805F4C879A33DBA32E618E018950A9@TRFT-EX01.xRothGen.nhs.uk> What - no durofix. I used them as a teenager. Oh, hang on - durofix. (UK only joke). Oh yes, used that too with Airfix models - hundreds of them. However, whatever was in the tube, and it could well have been durofix, that is not what it was called - it was a special histology stuff. Where is Kemlo when you want him? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian Montgomery Sent: 28 September 2007 17:02 To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] transfering tissue to another slide? Terry, The tube of "stuff" was Durofix. Cast your mind back to when you were a boy and built Airfix models, it's the same glue. Over the years I've searched for Durofix but think it's no longer on the market. Technique. 1.) Remove the coverglass by soaking in warm xylene or equivalent. 2.) Soak in fresh clearing agent until you are sure the mounting media has been removed. 3.) Cover the section with a mixture of 6 parts butyl-acetate, 1 part Durofix. 4.) Harden in a 37C oven for ~30 minutes. 5.) Carefully, with a sharp razor blade, cut the film from around the section. 6.) Place the slide in cold water and leave until the film with the section floats off. 7.) Mount on a clean slide. 8.) Drain excess water then place in a 37C oven until perfectly dry. 9.) Wash carefully with butyl-acetate. 10) Wash thoroughly in clearing agent and mount. When Durofix was available I used the technique on numerous occasions and with a few tweeks it worked well. All you need do is find glue that bonds plastics and you're away. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, G12 8QQ. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: 28 September 2007 15:30 To: Thom Jensen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] transfering tissue to another slide? Kemlo, We had a tube of "stuff" in Stoke which worked reasonably well. I have not come across it since. Remember the name? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thom Jensen Sent: 28 September 2007 15:09 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] transfering tissue to another slide? Hey, Has anyone ever taken tissue off one slide and transfering it to another? I think I remember someone mentioning it in the past. Anyone know anything? Thanks, Thom for information on instructing TMA go to: www.arrayworkshop.com New array instrument: www.arraymold.com _________________________________________________________________ Invite your mail contacts to join your friends list with Windows Live Spaces. It's easy! http://spaces.live.com/spacesapi.aspx?wx_action=create&wx_url=/friends.a spx&mkt=en-us_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Fri Sep 28 11:14:33 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Sep 28 11:14:39 2007 Subject: [Histonet] transfering tissue to another slide? References: Message-ID: <001a01c801ea$a89214a0$0302a8c0@yourxhtr8hvc4p> Thom, contact the people at New Comer's Supply newly@newcomersupply.com They have a mounting media called Mount Quick and a procedure. I have used it many times and it works great. I have taken pap smears and was able to get 3 slides. JTT ----- Original Message ----- From: "Thom Jensen" To: Sent: Friday, September 28, 2007 9:08 AM Subject: [Histonet] transfering tissue to another slide? Hey, Has anyone ever taken tissue off one slide and transfering it to another? I think I remember someone mentioning it in the past. Anyone know anything? Thanks, Thom for information on instructing TMA go to: www.arrayworkshop.com New array instrument: www.arraymold.com _________________________________________________________________ Invite your mail contacts to join your friends list with Windows Live Spaces. It's easy! http://spaces.live.com/spacesapi.aspx?wx_action=create&wx_url=/friends.aspx&mkt=en-us_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Fri Sep 28 11:14:43 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 28 11:15:02 2007 Subject: [Histonet] transfering tissue to another slide? Message-ID: <407F05A128805F4C879A33DBA32E618E018950AA@TRFT-EX01.xRothGen.nhs.uk> PS The procedure sound exactly the same! Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian Montgomery Sent: 28 September 2007 17:02 To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] transfering tissue to another slide? Terry, The tube of "stuff" was Durofix. Cast your mind back to when you were a boy and built Airfix models, it's the same glue. Over the years I've searched for Durofix but think it's no longer on the market. Technique. 1.) Remove the coverglass by soaking in warm xylene or equivalent. 2.) Soak in fresh clearing agent until you are sure the mounting media has been removed. 3.) Cover the section with a mixture of 6 parts butyl-acetate, 1 part Durofix. 4.) Harden in a 37C oven for ~30 minutes. 5.) Carefully, with a sharp razor blade, cut the film from around the section. 6.) Place the slide in cold water and leave until the film with the section floats off. 7.) Mount on a clean slide. 8.) Drain excess water then place in a 37C oven until perfectly dry. 9.) Wash carefully with butyl-acetate. 10) Wash thoroughly in clearing agent and mount. When Durofix was available I used the technique on numerous occasions and with a few tweeks it worked well. All you need do is find glue that bonds plastics and you're away. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, G12 8QQ. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: 28 September 2007 15:30 To: Thom Jensen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] transfering tissue to another slide? Kemlo, We had a tube of "stuff" in Stoke which worked reasonably well. I have not come across it since. Remember the name? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thom Jensen Sent: 28 September 2007 15:09 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] transfering tissue to another slide? Hey, Has anyone ever taken tissue off one slide and transfering it to another? I think I remember someone mentioning it in the past. Anyone know anything? Thanks, Thom for information on instructing TMA go to: www.arrayworkshop.com New array instrument: www.arraymold.com _________________________________________________________________ Invite your mail contacts to join your friends list with Windows Live Spaces. It's easy! http://spaces.live.com/spacesapi.aspx?wx_action=create&wx_url=/friends.a spx&mkt=en-us_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Fri Sep 28 11:22:06 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 28 11:22:12 2007 Subject: [Histonet] transfering tissue to another slide? Message-ID: <407F05A128805F4C879A33DBA32E618E018950AB@TRFT-EX01.xRothGen.nhs.uk> Sounds like instructions in a stud farm. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: 28 September 2007 17:15 To: Thom Jensen; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] transfering tissue to another slide? Thom, contact the people at New Comer's Supply newly@newcomersupply.com They have a mounting media called Mount Quick and a procedure. I have used it many times and it works great. I have taken pap smears and was able to get 3 slides. JTT ----- Original Message ----- From: "Thom Jensen" To: Sent: Friday, September 28, 2007 9:08 AM Subject: [Histonet] transfering tissue to another slide? Hey, Has anyone ever taken tissue off one slide and transfering it to another? I think I remember someone mentioning it in the past. Anyone know anything? Thanks, Thom for information on instructing TMA go to: www.arrayworkshop.com New array instrument: www.arraymold.com _________________________________________________________________ Invite your mail contacts to join your friends list with Windows Live Spaces. It's easy! http://spaces.live.com/spacesapi.aspx?wx_action=create&wx_url=/friends.a spx&mkt=en-us_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Fri Sep 28 11:32:02 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 28 11:32:07 2007 Subject: [Histonet] hydrogen peroxide quenching- shelf life Message-ID: A shelf-life doesn't mean much for a bottle of hydrogen peroxide once it has been opened. Decomposition (to water and oxygen) is catalyzed by traces of many substances, including metals and phosphate ions. In my lab, a long time ago, we used a 1ml syringe with a long needle to withdraw small volumes of 30% H2O2 for dilution. From time to time the stock bottle would die overnight; I suspect that this was due to contamination by traces of iron from the needle. Years ago we changed from 30% H2O2 to a solid compound called urea hydrogen peroxide (UHP). This is treated as 35% w/w H2O2. It seems to be very stable, and not disturbed by digging it out of the bottle with a nickel spatula. A 1% solution of H2O2 made from UHP (2.86 g in 100 ml water) can be kept on the bench for a few days and replaced before it has time to decompose. ----- Original Message ----- From: arvind Date: Friday, September 28, 2007 4:31 Subject: [Histonet] hydrogen peroxide quenching- shelf life To: Histonet@lists.utsouthwestern.edu > is there any shelf life for H2O2 so that it can be > conformed before > quenching unless its too late how can we know the purity of it > > *Arvind Singh Pundir* > National Brain Research Centre > Manesar, Gurgaon- Haryana- INDIA > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From schaundrawalton <@t> yahoo.com Fri Sep 28 11:45:57 2007 From: schaundrawalton <@t> yahoo.com (Schaundra Walton) Date: Fri Sep 28 11:46:00 2007 Subject: [Histonet] Neutralization of Formalin Message-ID: <795015.59075.qm@web58909.mail.re1.yahoo.com> Hi everyone! Our lab neutralizes our 10% formalin before dumping it down the drain. We use Formalex (American Master Tech) to neutralize and then add sodium hydroxide to balance the pH. We had been using residual formaldehyde test strips to test the ppm (Market Lab) before dumping, but they have been discontinued. We have tried to replace them with EM formaldehyde test strips (Fisher Scientific), but we are having trouble getting them to work. Anyone else neutralizing their formalin? What are you using and are you testing it before dumping it? Schaundra Walton BS HTL(ASCP) Swedish American Hospital 1401 E. State St. Rockford, IL 61104 --------------------------------- Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: mail, news, photos & more. From schaundrawalton <@t> yahoo.com Fri Sep 28 11:49:23 2007 From: schaundrawalton <@t> yahoo.com (Schaundra Walton) Date: Fri Sep 28 11:49:29 2007 Subject: [Histonet] Pneumocystis Controls Message-ID: <585652.66750.qm@web58908.mail.re1.yahoo.com> We order commercial Pneumocystis carnii (as well as AFB) control slides from Newcomer Supply. It seems they are having a shortage on tissue to make these controls and they are indefinately backordered. Anyone else using commercial controls? Where are you ordering from? Schaundra Walton BS HTL(ASCP) Swedish American Hospital 1401 E. State St. Rockford, IL 61104 --------------------------------- Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: mail, news, photos & more. From schaundrawalton <@t> yahoo.com Fri Sep 28 11:53:34 2007 From: schaundrawalton <@t> yahoo.com (Schaundra Walton) Date: Fri Sep 28 11:53:38 2007 Subject: [Histonet] Alcohol Recycling Message-ID: <983388.22246.qm@web58912.mail.re1.yahoo.com> Anyone out there recycling their alcohols? We have been trying to do this for about a year now. We looked at a CBG recycler, but were unhappy with it. For the past several months we have been trying to use Suncycle Technologies' GRAC-TP cartridges, but we have yet to have one last as long as promised. Anyone out there familiar with this company? Anyone else recycling with something else? Schaundra Walton BS HTL(ASCP) Swedish American Hospital 1401 E. State St. Rockford, IL 61104 --------------------------------- Yahoo! oneSearch: Finally, mobile search that gives answers, not web links. From jnocito <@t> satx.rr.com Fri Sep 28 11:55:19 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Sep 28 11:55:48 2007 Subject: [Histonet] transfering tissue to another slide? References: <001a01c801ea$a89214a0$0302a8c0@yourxhtr8hvc4p> Message-ID: <002a01c801f0$66ef0b60$0302a8c0@yourxhtr8hvc4p> I remember the procedure. 1. Take off the cover slip and soak in xylene or substitute for 15 minutes. 2. Apply enough Mount Quick to cover sections and place in an 80 degree oven for 30 minutes or until hardened. 3. place slide in tissue floatation bath for 1 hour or until the section can be lifted off with forceps. 4. wet slide(s) that will receive the transferred section with water. 5 remove the section carefully. If the section does not come off easy, keep in waterbath longer. This is important--- the side that was on the old slide must be placed on the slide exactly how it was removed because this will have a flat surface. 6. dry in oven again for 1 hour 7. clear, dehydrate and use as needed. One problem I encountered was with plus slides. I had to leave the slide in the floatation bath overnight to get better results, otherwise, only parts of the tissue would transfer. JTT ----- Original Message ----- From: "Joe Nocito" To: "Thom Jensen" ; Sent: Friday, September 28, 2007 11:14 AM Subject: Re: [Histonet] transfering tissue to another slide? > Thom, > contact the people at New Comer's Supply newly@newcomersupply.com > > They have a mounting media called Mount Quick and a procedure. I have used > it many times and it works great. I have taken pap smears and was able to > get 3 slides. > > JTT > > ----- Original Message ----- > From: "Thom Jensen" > To: > Sent: Friday, September 28, 2007 9:08 AM > Subject: [Histonet] transfering tissue to another slide? > > > > Hey, > Has anyone ever taken tissue off one slide and transfering it to another? > I think I remember someone mentioning it in the past. > > Anyone know anything? > > Thanks, > Thom > > > for information on instructing TMA go to: www.arrayworkshop.com > > New array instrument: www.arraymold.com > > > _________________________________________________________________ > Invite your mail contacts to join your friends list with Windows Live > Spaces. It's easy! > http://spaces.live.com/spacesapi.aspx?wx_action=create&wx_url=/friends.aspx&mkt=en-us_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mmarti <@t> cmrb.eu Fri Sep 28 11:59:28 2007 From: mmarti <@t> cmrb.eu (=?iso-8859-1?Q?Marti_Gaudes=2C_Merc=E8?=) Date: Fri Sep 28 11:59:24 2007 Subject: [Histonet] Gata4 Message-ID: Dear all I'm trying to detect Gata4 in heart tissue, without good results. I have tried one antibody from Santa Cruz and one from Chemicon, and in both cases I didn't found any signal. I'm doing different antigen retrievals. Can you suggest me some protocol? Thank you very much in advance. Merc? Mart? Gaudes Cap de Servei Histology and Bioimaging Centre de Medicina Regenerativa de Barcelona (CMR[B]) Dr. Aiguader, 88 7ena planta 08003 Barcelona mmarti@cmrb.eu Tel: +34 93 316 03 51 Fax: +34 93 224 10 83 From gu.lang <@t> gmx.at Fri Sep 28 12:01:08 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Sep 28 12:01:35 2007 Subject: AW: [Histonet] Immuno's on Thin Prep specimens In-Reply-To: Message-ID: <000901c801f1$2a887070$6412a8c0@dielangs.at> I got the recommendation to fix the slides for 10-20 min in NBF and run them then in the same way as the paraffin slides. The pro is, that you don't have to modify your usual protocols. Also it has been said, that some of the antibodies bring better results with HIER, even if there was no formalinfixation first. But my personal exerperience is very small. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von AWeiss@shorememorial.org Gesendet: Freitag, 28. September 2007 13:24 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Immuno's on Thin Prep specimens Is anyone doing IHC's on cytology specimens? If you are can you please send staining and fixation protocol. Also, what do you use for your positive and negative controls? Thank you Andrea J Weiss BST CT (ASCP) Cytotechnologist 609 653 3577 Ext 4907 aweiss@shorememorial.org This transmittal from Shore Memorial Health System is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review or use, including disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender and destroy all copies of the transmittal. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Fri Sep 28 12:08:53 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Sep 28 12:09:10 2007 Subject: AW: [Histonet] Immuno's on Thin Prep specimens In-Reply-To: <46FCD10D.CC0E.007B.0@vetmed.ufl.edu> Message-ID: <000a01c801f2$41420be0$6412a8c0@dielangs.at> I think a part of the HIER action is really restoring: Because the methylenbridges are linked between the proteins and therefore also between the antigen-aminoacids. This causes a change in the structure of the protein and this can cause the "hiding" of the epitop. Paratop doesn't recognise epitop. After HIER the antigen-aminoacids restore there former structure. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Shawn Leslie Gesendet: Freitag, 28. September 2007 16:02 An: histonet@lists.utsouthwestern.edu; AWeiss@shorememorial.org; Sebree Linda A.; Rene J Buesa Betreff: RE: [Histonet] Immuno's on Thin Prep specimens Just a correction Rene. You stated "HIER is designed to "restore" the epitope reactive characteristics after being cross-linked by the paraffin ". Actually HIER was introduced to demask the epitope after being cross-linked (methylene bridges) by the formalin not the paraffin.... Just wanted make that correction Shawn Leslie Scientific Research Manager Anatomic Pathology University of Florida School of Veterinary Medicine 352-392-2235 ext 4555 >>> Rene J Buesa 9/28/2007 9:43 AM >>> Linda: Not to start a controversy, just to discuss the issue: 1- antibodies are designed to reveal the presence of a given epitope; 2- such epitopes are the same regardless of the processing protocol or the tissues they are in, either smears, thin-preps or tissues, they just vary in concentration and distribution among the cells; 3- HIER is designed to "restore" the epitope reactive characteristics after being cross-linked by the paraffin to its "original" or "natural" condition; 4-once the epitope has been restored it is equivalent to not having ever being altered and will react identically whenever it is present. IF your usual protocol to detect the epitope in your positive tissue control is not altered from its usual way and renders the same reaction intensitywise on the same target cells, it should also react in the thin prep treated identically. I realize that since you "recently stuck your head" on this issue and refused to do something like I am advocating, it will be rather difficult for you to analyze the precedent paragraphs passionlessly. but give a chance to reasoning. On the other hand, I have done hundreds of IHC on thin preps and always used FFPE tissue sections as positive controls. The positive controls always behaved as expected and the thin preps, treated simultaneously rendered positive or negative results, according with their specific nature. The thing that you have to be consistent with is using another identical thin prep as negaive control. Ren? J. "Sebree Linda A." wrote: I disagree Rene in terms of using a paraffin section as a positive control. Your two specimens (thin prep & + control) are not prepared the same. In order to get your + control to stain , you may have to employ HIER which as you state you would not do with the thin prep. That pretreatment of the + control makes it even "more different" than your patient unknown. I recently stuck my neck out at work for this very same issue. I refused to be part of running a cytospin with a FFPE staining protocol and + control. Our lab used to run cytospin IHC protocols and many times had to adjust the FFPE protocols to work for the cytopsins. At that time we used frozen section tissue controls for our positives for lack of a better alternative. These came closer to the patient specimen than FFPE controls and actually stained identically to the cytospins. Just my experience. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, September 28, 2007 7:49 AM To: AWeiss@shorememorial.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Immuno's on Thin Prep specimens If using thin preps for IHC, you do NOT need to do HIER because they are acetone/air dried fixed, not formalin fixed. The rest of the IHC protocol is the same as for a FFPE tissue section. Your positive control refers to the epitope, so your regular positive control from a tissue section can be used, BUT you negative control has to be a thin prep from the same case simultaneously prepared. Ren? J. AWeiss@shorememorial.org wrote: Is anyone doing IHC's on cytology specimens? If you are can you please send staining and fixation protocol. Also, what do you use for your positive and negative controls? Thank you Andrea J Weiss BST CT (ASCP) Cytotechnologist 609 653 3577 Ext 4907 aweiss@shorememorial.org This transmittal from Shore Memorial Health System is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review or use, including disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender and destroy all copies of the transmittal. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Need a vacation? Get great deals to amazing places on Yahoo! Travel. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Tonight's top picks. What will you watch tonight? Preview the hottest shows on Yahoo! TV. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bob.nienhuis <@t> gmail.com Fri Sep 28 12:29:44 2007 From: bob.nienhuis <@t> gmail.com (Bob Nienhuis) Date: Fri Sep 28 12:29:52 2007 Subject: [Histonet] Neutralization of Formalin In-Reply-To: <795015.59075.qm@web58909.mail.re1.yahoo.com> References: <795015.59075.qm@web58909.mail.re1.yahoo.com> Message-ID: <45109da50709281029n3fef9adbmfeaaa25c2565d781@mail.gmail.com> We used to do that, but are not allowed to do it anymore. Apparently, the Formulex produces some stuff that is nastier than the original formalin, We send to a waste disposal company. Bob UCLA / VA Medical Center Sepulveda, CA On 9/28/07, Schaundra Walton wrote: > > Hi everyone! > > Our lab neutralizes our 10% formalin before dumping it down the > drain. We use Formalex (American Master Tech) to neutralize and then add > sodium hydroxide to balance the pH. We had been using residual formaldehyde > test strips to test the ppm (Market Lab) before dumping, but they have been > discontinued. We have tried to replace them with EM formaldehyde test > strips (Fisher Scientific), but we are having trouble getting them to > work. Anyone else neutralizing their formalin? What are you using and are > you testing it before dumping it? > > > Schaundra Walton BS HTL(ASCP) > Swedish American Hospital > 1401 E. State St. > Rockford, IL 61104 > > --------------------------------- > Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: mail, > news, photos & more. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From vazquezr <@t> ohsu.edu Fri Sep 28 12:35:02 2007 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Sep 28 12:35:28 2007 Subject: [Histonet] Alcohol Recycling Message-ID: Shraundra, We use Creative Waste recycling system...it is awesome... 888-795-8300 ask for Rex He is coming to see me today! Robyn OHSU From laurie.colbert <@t> huntingtonhospital.com Fri Sep 28 13:19:43 2007 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Fri Sep 28 13:19:50 2007 Subject: [Histonet] transfering tissue to another slide? Message-ID: <57BE698966D5C54EAE8612E8941D768301BCBA3F@EXCHANGE3.huntingtonhospital.com> Thom, I also use the Mount Quick from Newcomer Supply. It works very well for us. I like to give plenty of time for each step, so it is usually a two-day procedure for us (but can be done faster). Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thom Jensen Sent: Friday, September 28, 2007 7:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] transfering tissue to another slide? Hey, Has anyone ever taken tissue off one slide and transfering it to another? I think I remember someone mentioning it in the past. Anyone know anything? Thanks, Thom for information on instructing TMA go to: www.arrayworkshop.com New array instrument: www.arraymold.com _________________________________________________________________ Invite your mail contacts to join your friends list with Windows Live Spaces. It's easy! http://spaces.live.com/spacesapi.aspx?wx_action=create&wx_url=/friends.a spx&mkt=en-us_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wilson_c <@t> ricerca.com Fri Sep 28 13:28:01 2007 From: wilson_c <@t> ricerca.com (Wilson, Carol) Date: Fri Sep 28 13:28:09 2007 Subject: [Histonet] Flash freezing tissue for transport to be cryostat sectioned at a later time Message-ID: <9D443EB9D0270143B5AAF190CB1A58A305064F60@dogwood.ricerca.com> Hi all, How would anyone out there suggest freezing various pieces of cat tissue to be transported to another laboratory on dry ice for cryostat sectioning when it gets to its destination? We don't have a cryostat or chucks on site, liquid nitrogen available. We need to be able to keep identity of each piece. Suggestions quick please as the necropsy team wants to stuff pieces of tissue into cryo vials and the pieces are way to big to be stuffed and will certainly end up crushed. Thanks, Carol Carol Wilson Team Leader - Histology From gvdobbin <@t> ihis.org Fri Sep 28 13:41:44 2007 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Fri Sep 28 13:42:04 2007 Subject: [Histonet] Pneumocystis Controls Message-ID: Hi Schaundra, Check with your nearest Veterinary College histology /pathology dept. Our positive controls are from the lungs of pigs infected with P. Carinii. Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 There is some merit in doing the right thing rather badly, but absolutely none in doing the wrong thing excellently! >>> Schaundra Walton 9/28/2007 1:49:23 PM >>> We order commercial Pneumocystis carnii (as well as AFB) control slides from Newcomer Supply. It seems they are having a shortage on tissue to make these controls and they are indefinately backordered. Anyone else using commercial controls? Where are you ordering from? Schaundra Walton BS HTL(ASCP) Swedish American Hospital 1401 E. State St. Rockford, IL 61104 --------------------------------- Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: mail, news, photos & more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ayant pour objet une personne ou un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer compl?tement de votre syst?me informatique. From Anthony.Gatt <@t> jefferson.edu Fri Sep 28 13:56:43 2007 From: Anthony.Gatt <@t> jefferson.edu (Anthony Gatt) Date: Fri Sep 28 13:48:41 2007 Subject: [Histonet] Bouin's fixative Message-ID: <20070928145643.AKO66688@logan.jefferson.edu> Hi. I was given a protocol for Drosophila head fixation that involved Bouin's fixative. After fixing for 48h, it says to put the samples into a leaching buffer (50mM Tris/150mM NaCl) overnight. What is the purpose of this buffer? From tkngflght <@t> yahoo.com Fri Sep 28 13:51:56 2007 From: tkngflght <@t> yahoo.com (Cheryl R. Kerry) Date: Fri Sep 28 13:51:52 2007 Subject: [Histonet] Flash freezing tissue for transport to be cryostatsectioned at a later time In-Reply-To: <9D443EB9D0270143B5AAF190CB1A58A305064F60@dogwood.ricerca.com> Message-ID: <012701c80200$a5728320$6401a8c0@CHERYLSLAPTOP> Carol-- For routine frozens, you don't need liquid nitrogen --just a really good source of COLD. Snap freezing is for certain things like muscle biopies--not everything. If you don't need snap freezing, we need to know what sources of 'cold' you do have? A -70 freezer would do it--or even slightly warmer than this--but colder than a regular home frige. This might not be a solution for you as it appears you're doing research--do you have some practice tissue to see if this will work without significant artifact? You can purchase frozen embedding molds from Surgipath and a few other vendors. They're plastic like disposable paraffin molds and come in various sizes. They're like a cassette without a lid and have a lip on which to write with a sharpie or histomarker. Paraffin disposable molds will work, but they crack and don't have as much room for writing. Put in your tissue, surround with OCT but don't over-fill, place on the coldest horizontal surface of the interior of your -70 and close the door. Once frozen, transport them. Before mounting on the chuck, score the surface of the frozen OCT so the new liquid OCT has something to grab onto. These mounts will break off if handled too aggressively so by roughing up the surface, the weakest union isn't the one you just created. Let the tissue in the frozen OCT and the new 'mounting' OCT harden/freeze and come to ambient temp in the cryostat and cut. If you cut more than one block at a time add a little piece of paper into the side of the liquid mounting OCT to carry the label once removed from the mold, the way we used to before cassettes were created. Label the paper with the tissue ID sticking out the side, if mounted in a downward position, won't get in the way of the knife and will stay put until you thaw. If you don't have an electrical source of cold, dry ice would work for smaller bits--but not so well with the larger ones. And don't forget safety with this cold stuff!! Practice a mock run before doing important tissue, too, in case the process needs tweaking or just plain fails! Hope this helps! Cheryl Cheryl R. Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab, one great tech at a time. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wilson, Carol Sent: Friday, September 28, 2007 1:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Flash freezing tissue for transport to be cryostatsectioned at a later time Hi all, How would anyone out there suggest freezing various pieces of cat tissue to be transported to another laboratory on dry ice for cryostat sectioning when it gets to its destination? We don't have a cryostat or chucks on site, liquid nitrogen available. We need to be able to keep identity of each piece. Suggestions quick please as the necropsy team wants to stuff pieces of tissue into cryo vials and the pieces are way to big to be stuffed and will certainly end up crushed. Thanks, Carol Carol Wilson Team Leader - Histology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gvdobbin <@t> ihis.org Fri Sep 28 13:54:13 2007 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Fri Sep 28 13:54:31 2007 Subject: [Histonet] Flash freezing tissue for transport to be cryostat sectioned at a later time Message-ID: Hi Carol, I guess "quick" is no longer applicable here, but when you have time, order the old fashion Peel-A-Way molds. Label each mold, place a little OCT in the bottom and then add the tissue. Float thease molds then on a foil boat in a safe receptical for liq. N2. As the bottom of the mold starts to freeze, cover the tissue with more OCT and allow to freeze completely. The tissue is now labelled, safe from freeze drying and ready for mounting on a chuck (once the mold is removed of course) and cutting. See full description in Biotechnic & Histochemistry 2003, 78(5): 279. "Notes and queries" section. Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 There is some merit in doing the right thing rather badly, but absolutely none in doing the wrong thing excellently! >>> "Wilson, Carol" 9/28/2007 3:28:01 PM >>> Hi all, How would anyone out there suggest freezing various pieces of cat tissue to be transported to another laboratory on dry ice for cryostat sectioning when it gets to its destination? We don't have a cryostat or chucks on site, liquid nitrogen available. We need to be able to keep identity of each piece. Suggestions quick please as the necropsy team wants to stuff pieces of tissue into cryo vials and the pieces are way to big to be stuffed and will certainly end up crushed. Thanks, Carol Carol Wilson Team Leader - Histology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ayant pour objet une personne ou un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer compl?tement de votre syst?me informatique. From brett_connolly <@t> merck.com Fri Sep 28 15:00:08 2007 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Fri Sep 28 15:01:02 2007 Subject: [Histonet] Mammaglobin A & GCDFP-15 Message-ID: <63EA0607835FBA4689CEA9EA8B48269282937B@usctmx1141.merck.com> Hi all, Looking for suppliers of these antibodies for FFPE tissues. Also, necessary pretreatments/dilutions used. Thanks, Brett Brett M. Connolly, Ph.D. Research Fellow MRL, Imaging Research Merck & Co., Inc. WP-44K PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com ------------------------------------------------------------------------------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------------ From mcauliff <@t> umdnj.edu Fri Sep 28 15:04:41 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 28 15:05:00 2007 Subject: [Histonet] Bouin's fixative In-Reply-To: <20070928145643.AKO66688@logan.jefferson.edu> References: <20070928145643.AKO66688@logan.jefferson.edu> Message-ID: <46FD5E59.1050801@umdnj.edu> It is important to wash excess picrates out of the tissue after Bouin's fixation. Most people use several changes of 50% ethanol, one hour each. I suppose the "leaching buffer" is intended to do the same thing? Geoff Anthony Gatt wrote: > Hi. I was given a protocol for Drosophila head fixation that involved Bouin's fixative. After fixing for 48h, it says to put the samples into a leaching buffer (50mM Tris/150mM NaCl) overnight. What is the purpose of this buffer? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From b-frederick <@t> northwestern.edu Fri Sep 28 15:46:17 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Sep 28 15:46:31 2007 Subject: [Histonet] Bouin's fixative In-Reply-To: <46FD5E59.1050801@umdnj.edu> Message-ID: <000101c80210$a13e5c10$d00f7ca5@lurie.northwestern.edu> We rinse it in running water. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Geoff McAuliffe Sent: Friday, September 28, 2007 3:05 PM To: Anthony.Gatt@jefferson.edu Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Bouin's fixative It is important to wash excess picrates out of the tissue after Bouin's fixation. Most people use several changes of 50% ethanol, one hour each. I suppose the "leaching buffer" is intended to do the same thing? Geoff Anthony Gatt wrote: > Hi. I was given a protocol for Drosophila head fixation that involved Bouin's fixative. After fixing for 48h, it says to put the samples into a leaching buffer (50mM Tris/150mM NaCl) overnight. What is the purpose of this buffer? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SDrew <@t> uwhealth.org Fri Sep 28 15:52:19 2007 From: SDrew <@t> uwhealth.org (Drew Sally A.) Date: Fri Sep 28 15:52:25 2007 Subject: [Histonet] IHC Forum subject? Message-ID: Maybe the whole discussion of the proper way to "control" IHC on cytospins, ThinPreps, etc. could be brought up at this forum? Sally Ann Drew, MT(ASCP) IHC/ISH Laboratory University of Wisconsin Hosp. & Clinics Madison, WI 53792 (608)265-6596 From LSebree <@t> uwhealth.org Fri Sep 28 16:09:11 2007 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Fri Sep 28 16:09:15 2007 Subject: [Histonet] Reference lab doing IHC on cytospins Message-ID: Hello 'netters: We're wondering if there's a reference lab out there that does immunohistochemistry testing on cytology preparations. Have a good weekend all! Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From igor.deyneko <@t> gmail.com Fri Sep 28 16:47:18 2007 From: igor.deyneko <@t> gmail.com (Igor Deyneko) Date: Fri Sep 28 16:47:24 2007 Subject: [Histonet] Polymer Paraffin Message-ID: <35e16a770709281447g2b817dc5w77f6b7664776e873@mail.gmail.com> Dear Histonetters! I was just wondering, can someone advise a good embedding paraffin for me? I embed tumors mostly, and occasional livers and pancreas, and very rarely colon. The 1 I'm using right now, Richard-Allen #6, I'm not crazy about. I am looking for one preferably with polymers. Has anyone ever used the SurgiPath Embedding paraffin and what are your reflections? Thank you. Igor Deyneko. Infinity Pharmaceuticals From rjbuesa <@t> yahoo.com Fri Sep 28 16:49:16 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Sep 28 16:49:21 2007 Subject: [Histonet] IHC Forum subject? In-Reply-To: Message-ID: <177202.34585.qm@web61224.mail.yahoo.com> It would be an extremely interesting and very useful discussion. Pitty I could not attend! Besides the intelectual reasoning of the issues, some hard data/evidence should be included, or programmed for a future meeting. Ren? J. "Drew Sally A." wrote: Maybe the whole discussion of the proper way to "control" IHC on cytospins, ThinPreps, etc. could be brought up at this forum? Sally Ann Drew, MT(ASCP) IHC/ISH Laboratory University of Wisconsin Hosp. & Clinics Madison, WI 53792 (608)265-6596 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Catch up on fall's hot new shows on Yahoo! TV. Watch previews, get listings, and more! From rjbuesa <@t> yahoo.com Fri Sep 28 16:50:43 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Sep 28 16:50:47 2007 Subject: [Histonet] Polymer Paraffin In-Reply-To: <35e16a770709281447g2b817dc5w77f6b7664776e873@mail.gmail.com> Message-ID: <237432.36263.qm@web61214.mail.yahoo.com> Or infiltrating/embedding paraffin was Paraplast-Plus, and we used with very good results. Ren? j. Igor Deyneko wrote: Dear Histonetters! I was just wondering, can someone advise a good embedding paraffin for me? I embed tumors mostly, and occasional livers and pancreas, and very rarely colon. The 1 I'm using right now, Richard-Allen #6, I'm not crazy about. I am looking for one preferably with polymers. Has anyone ever used the SurgiPath Embedding paraffin and what are your reflections? Thank you. Igor Deyneko. Infinity Pharmaceuticals _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. From bob.nienhuis <@t> gmail.com Fri Sep 28 19:02:29 2007 From: bob.nienhuis <@t> gmail.com (Bob Nienhuis) Date: Fri Sep 28 19:02:34 2007 Subject: [Histonet] Neutralization of Formalin In-Reply-To: <45109da50709281654l6298a7feu31e4b56824024769@mail.gmail.com> References: <45109da50709281029n3fef9adbmfeaaa25c2565d781@mail.gmail.com> <45109da50709281654l6298a7feu31e4b56824024769@mail.gmail.com> Message-ID: <45109da50709281702h16bc77a9g51eb5eb29c28b840@mail.gmail.com> See attached memo from the California State EPA re: Notice of Intent to Deny Re-Certification Hazardous Waste Environmental Technology. Bob UCLA / VA Medical Center Sepulveda, CA On 9/28/07, Rathborne, Toni wrote: > > Bob, > > Please explain. We use Formalex too. > > Thanks, > Toni Rathborne > Pathology Supervisor > Somerset Medical Center > 110 Rehill Ave. > Somerville,NJ 08876 > 908-595-2367 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu ]On Behalf Of Bob > Nienhuis > Sent: Friday, September 28, 2007 1:30 PM > To: Schaundra Walton > Cc: Histonet > Subject: Re: [Histonet] Neutralization of Formalin > > > We used to do that, but are not allowed to do it anymore. Apparently, the > Formulex produces some > stuff that is nastier than the original formalin, We send to a waste > disposal company. > > Bob > UCLA / VA Medical Center > Sepulveda, CA > > On 9/28/07, Schaundra Walton < schaundrawalton@yahoo.com> wrote: > > > > Hi everyone! > > > > Our lab neutralizes our 10% formalin before dumping it down the > > drain. We use Formalex (American Master Tech) to neutralize and then > add > > sodium hydroxide to balance the pH. We had been using residual > formaldehyde > > test strips to test the ppm (Market Lab) before dumping, but they have > been > > discontinued. We have tried to replace them with EM formaldehyde test > > strips (Fisher Scientific), but we are having trouble getting them to > > work. Anyone else neutralizing their formalin? What are you using and > are > > you testing it before dumping it? > > > > > > Schaundra Walton BS HTL(ASCP) > > Swedish American Hospital > > 1401 E. State St. > > Rockford, IL 61104 > > > > --------------------------------- > > Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: > mail, > > news, photos & more. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From koellingr <@t> comcast.net Sat Sep 29 15:45:21 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Sat Sep 29 15:45:30 2007 Subject: [Histonet] OT and GATA Message-ID: <092920072045.888.46FEB9610004A8C10000037822070245539D09020704040A0105@comcast.net> Merc Marti Gaudes- I have copied and pasted a message to just the histonet that came back at me after I attempted to send it to you and histonet. My wife writes in Japanese kanji on this computer and apparently the computer or server was really unhappy sending this to you or receiving it because it substituted Japanese characters in the address and sent a fatal flaw back to me. So here is what I sent earlier: Merc Marti Gaudes- Is this just normal heart tissue? Mouse or human? GATA4 is zinc-finger transcriptional factor, so found in nucleus and if you are counterstaining, depending on how you are counterstaining, if the signal is weak you can cover it up. Have used a Santa Cruz antibody (although for personal reasons I only use SC and their famous herd of goats, if I'm desperate to the final degree). Have used eBiosciences and Lifespan Biosciences anti GATA4. Usually a citrate buffer/microwave or steam but certainly there are other ways you can go. Just remember, this is a zinc-finger motif protein so might stand up to all pre-treatments. So it can easily be seen and I've done it, in the granulosa cells of ovary and developing heart. Was purported to be an early cardiomyocyte differentiation factor and marker but I haven't kept up on the literature and believe it has been found elsewhere (normally and in tumors or pathological states) although you'd nee d to check the literature to find out where. I just haven't kept up. But I've seen it in cell lines, developing heart and ovary very nicely with the above antibodies and pretreatment. Since the protein is highly conserved to the consensus DNA sequence G-A-T-A, the antibodies work in mouse or human or other species. I'd try looking at mouse developing heart to make sure my system is working for it easy to detect there. dull Ray Koelling PhenoPath Laboratories Seattle, WA From koellingr <@t> comcast.net Sat Sep 29 16:13:35 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Sat Sep 29 16:13:41 2007 Subject: [Histonet] smad 6&7 controls Message-ID: <092920072113.11362.46FEBFFF00003CC600002C6222007504389D09020704040A0105@comcast.net> Louise, Not currently working on it but formerly so this is what I can tell you. SMAD's are involved in regulatory cellular signalling by TGF-beta and as that was a big area of interest for a former company, this information is from those times. These are intimately involved in cartilage and bone metabolism and development so your controls might be from bone (mine were plus cell lines) although there certainly are other possibilities. I pulled from my file a place you might start and is from an orthopedic group. See Journal of Histochemistry and Cytochemistry in 2005 a really nice report Tasima Hague is first author so easy to PubMed it up. They did IHC stain for SMAD 6 and 7, have tissues, antibodies, controls, pictures, etc listed so that article can tell you a lot of the information as a starting point. (OT so do not read on if uninterested). SMAD6 stands for "mothers against decapentaplegic homolog 6". SMAD was a combination word from a Drosophila MAD gene and a homolog c.elega ns gene that started with letter "S" and I forgot what it was. That brings up the whole concept of the problem of nomenclature of genes as they have been discovered in various species yeast, fruit flies, frogs, fish and mammals and there were attempts to relate them across species. In fact, in the late 1990's I believe, when this random naming was out of control, a group got together to attempt to bring some standardiztion to the naming of genes (much like when in the early stages of monoclonal antibody development naming became chaotic until the International Workshops started the CD marker system to bring sanity to the chaotic nomenclature). But the gene people weren't so successful and although there are some standard naming protocols within species, I believe one of the statements they made was something like naming genes and attrributing or relating them across species was essentially hopeless and useless. So we are stuck with SMAD and Smurf (Smad ubiguiting regulatory fac tors), Hedgehog (and then Sonic Hedgehog and others), sevenless (and then son of sevenless SOS and daughter of sevenless DOS and daughters and sons and mothers of descriptive genes that is completely up to the whimsical nature of the discoverer. But get that paper from 2005 and it will help you a lot. dull Raymond Koelling PhenoPath Laboratories Seattle, WA -------------- Original message -------------- From: "louise renton" > Hi there, > > is ANYONE out there doing immuno on the above (or knows something about > these proteins) who could suggest which tissues I could use for controls? > > Much appreciated > > -- > Louise Renton > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > "There are nights when the wolves are silent and only the moon howls". > George Carlin > No trees were killed in the sending of this message. > However, many electrons were terribly inconvenienced. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bob.nienhuis <@t> gmail.com Sat Sep 29 17:53:57 2007 From: bob.nienhuis <@t> gmail.com (Bob Nienhuis) Date: Sat Sep 29 17:54:11 2007 Subject: [Histonet] Neutralization of Formalin In-Reply-To: <45109da50709281702h16bc77a9g51eb5eb29c28b840@mail.gmail.com> References: <45109da50709281029n3fef9adbmfeaaa25c2565d781@mail.gmail.com> <45109da50709281654l6298a7feu31e4b56824024769@mail.gmail.com> <45109da50709281702h16bc77a9g51eb5eb29c28b840@mail.gmail.com> Message-ID: <45109da50709291553rbc67d6aw97c7f1a339cc2317@mail.gmail.com> Oops. I guess I can't attach files to Histonet messages. I have uploaded a copy of the Calif. EPA Formalex de-certification memo to: Hope this works. Bob UCLA / VA Medical Center Sepulveda, CA On 9/28/07, Bob Nienhuis wrote: > > > > See attached memo from the California State EPA re: Notice of Intent to > Deny Re-Certification > Hazardous Waste Environmental Technology. > > Bob > UCLA / VA Medical Center > Sepulveda, CA > > On 9/28/07, Rathborne, Toni wrote: > > > > Bob, > > > > Please explain. We use Formalex too. > > > > Thanks, > > Toni Rathborne > > Pathology Supervisor > > Somerset Medical Center > > 110 Rehill Ave. > > Somerville,NJ 08876 > > 908-595-2367 > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto: histonet-bounces@lists.utsouthwestern.edu ]On Behalf Of Bob > > Nienhuis > > Sent: Friday, September 28, 2007 1:30 PM > > To: Schaundra Walton > > Cc: Histonet > > Subject: Re: [Histonet] Neutralization of Formalin > > > > > > We used to do that, but are not allowed to do it anymore. Apparently, > > the > > Formulex produces some > > stuff that is nastier than the original formalin, We send to a waste > > disposal company. > > > > Bob > > UCLA / VA Medical Center > > Sepulveda, CA > > > > On 9/28/07, Schaundra Walton < schaundrawalton@yahoo.com> wrote: > > > > > > Hi everyone! > > > > > > Our lab neutralizes our 10% formalin before dumping it down the > > > drain. We use Formalex (American Master Tech) to neutralize and then > > add > > > sodium hydroxide to balance the pH. We had been using residual > > formaldehyde > > > test strips to test the ppm (Market Lab) before dumping, but they have > > been > > > discontinued. We have tried to replace them with EM formaldehyde test > > > > > strips (Fisher Scientific), but we are having trouble getting them to > > > work. Anyone else neutralizing their formalin? What are you using > > and are > > > you testing it before dumping it? > > > > > > > > > Schaundra Walton BS HTL(ASCP) > > > Swedish American Hospital > > > 1401 E. State St. > > > Rockford, IL 61104 > > > > > > --------------------------------- > > > Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: > > mail, > > > news, photos & more. > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > From amosbrooks <@t> gmail.com Sat Sep 29 22:02:09 2007 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sat Sep 29 22:02:18 2007 Subject: [Histonet] transfering tissue to another slide? Message-ID: <582736990709292002v231f08e8k6ed7384069c07643@mail.gmail.com> Thom, Use a product from Newcomer Supply http://www.newcomersupply.com/labdevices.html called Mount Quick. You can cover the sections with a gel like cover and float them off with the tissues intact, separate serial sections and pick them up on separate slides. It is an awesome product. Look for it with mounting media. good luck, Amos Message: 2 Date: Fri, 28 Sep 2007 14:08:53 +0000 From: Thom Jensen Subject: [Histonet] transfering tissue to another slide? To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hey, Has anyone ever taken tissue off one slide and transfering it to another? I think I remember someone mentioning it in the past. Anyone know anything? Thanks, Thom From ancillarypath <@t> mac.com Sat Sep 29 23:42:23 2007 From: ancillarypath <@t> mac.com (ancillarypath@mac.com) Date: Sat Sep 29 23:42:41 2007 Subject: [Histonet] Announcing IHC Tech position at Vitro Molecular Laboratories In-Reply-To: <200709291706.l8TH6H5N000721@mac.com> References: <200709291706.l8TH6H5N000721@mac.com> Message-ID: Dear colleagues, We are a new job posting for an experienced IHC technologist as a full-time position and another part-time position at our laboratory in Miami. Job requirements include good experience in IHC techniques, including double-staining, manual staining, troubleshooting, making dilutions and buffers. The salary is generous, and it is proportional to the level and years of experience, with an attractive benefit package. If you think you are qualified and interested in this position, please send me immediately your cv along with 3 references and we will contact you as soon as we can. Best regards, ================================ Hadi Yaziji, M.D., Medical Director Vitro Molecular Laboratories President, Ancillary Pathways 7000 62nd Avenue, PH-C Miami, FL 33143 T 305-740-4440 F. 786-513-0175 www.vitromolecular.com www.ancillarypath.com From rmoncayo20 <@t> yahoo.com Sun Sep 30 11:49:18 2007 From: rmoncayo20 <@t> yahoo.com (Hector Raul Moncayo Oquendo) Date: Sun Sep 30 11:49:28 2007 Subject: [Histonet] I need help you By Shandon Varistain 24-4 Message-ID: <238466.45781.qm@web35608.mail.mud.yahoo.com> Hi I have a problem with slide retainer Varistain 24-4 Sahndon. When I will ON appears mmesage ERR 01 FAIL. Are you would help me Best Regards Hector Moncayo Lima Peru Southamerica ____________________________________________________________________________________ ?S? un mejor ambientalista! Encuentra consejos para cuidar el lugar donde vivimos. http://telemundo.yahoo.com/promos/mejorambientalista.html From pruegg <@t> ihctech.net Sun Sep 30 12:23:44 2007 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Sun Sep 30 12:19:56 2007 Subject: [Histonet] IHC Forum Message-ID: <200709301719.l8UHJhhD018924@pro12.abac.com> This is a call for IHC questions to be discussed at the NSH S/C in Denver in OCT. If you have any burning questions you would like our panel of 9 to address please email them to me ahead of time. Thank you, Patsy Ruegg Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net > Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net From pruegg <@t> ihctech.net Sun Sep 30 12:57:05 2007 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Sun Sep 30 12:53:16 2007 Subject: [Histonet] Polymer Paraffin In-Reply-To: <237432.36263.qm@web61214.mail.yahoo.com> Message-ID: <200709301753.l8UHr48K038999@pro12.abac.com> I agree I use paraplast plus which I now get from Stat Labs, it is a McCormick brand. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, September 28, 2007 3:51 PM To: Igor Deyneko; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Polymer Paraffin Or infiltrating/embedding paraffin was Paraplast-Plus, and we used with very good results. Ren? j. Igor Deyneko wrote: Dear Histonetters! I was just wondering, can someone advise a good embedding paraffin for me? I embed tumors mostly, and occasional livers and pancreas, and very rarely colon. The 1 I'm using right now, Richard-Allen #6, I'm not crazy about. I am looking for one preferably with polymers. Has anyone ever used the SurgiPath Embedding paraffin and what are your reflections? Thank you. Igor Deyneko. Infinity Pharmaceuticals _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sun Sep 30 13:03:18 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Sep 30 13:03:24 2007 Subject: [Histonet] IHC Forum In-Reply-To: <200709301719.l8UHJhhD018924@pro12.abac.com> Message-ID: <513570.38831.qm@web61211.mail.yahoo.com> Hi Patsy: This week there was a discussion in Histonet about IHC on PAP smears and cytospins and using tissue sections as positive controls or not. Roughly the positions were the following: 1- some accepted using FFPE tissue sections as (+) controls, after HIER 2- some suggested fixing the smears with NBF and cause the "crossslinkage" to do HIER afterwards 3- some advocated using ONLY known (+) smears or frozen sections as (+) controls, i.e. non-fixed tissues. The rational being a CAP instruction in which they direct to use controls treated the same as the case. I think this is an important topic but I also think the "discussion" should be beyond an intelectual argument of each, because that will just expand what has been said this last week. I think that with some time ahead some "hard evidence" ought to be presented for discussion, i.e. results obtained using the 3 "approaches" summarized above. The panel then should view the slides, grade them and get to a determination. The slides should be reviewed/graded "blind", i.e. no one shoudl know the procedure s/he is evaluating. Give consideration to this proposal. Ren? J. patsy ruegg wrote: This is a call for IHC questions to be discussed at the NSH S/C in Denver in OCT. If you have any burning questions you would like our panel of 9 to address please email them to me ahead of time. Thank you, Patsy Ruegg Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net > Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better Globetrotter. Get better travel answers from someone who knows. Yahoo! Answers - Check it out. From Shirley_PHUA <@t> hsa.gov.sg Sun Sep 30 13:02:55 2007 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Sun Sep 30 13:09:34 2007 Subject: [Histonet] Shirley Phua is away on 01-02 October 2007 (Monday & Tuesday). Message-ID: I will be out of the office from 01-10-2007 to 02-10-2007. I'll be away on 01-02 October 2007 (Monday & Tuesday). I'll be back on 03 October 2007 (Wednesday). Pathologists: I will process your requests when I return. If urgent, please forward your email to Henry_Kyaw@hsa.gov.sg