[Histonet] background staining after DAB

[Diana McCaig]

Hi
We are using the Biocare IHC protocol and using Fisher superfrost plus
slides (due to decloaking).  When doing the double stain, there is a lot
of background stain from the DAB.  We do wash well so that is not the
problem.  I am wondering if is the charge on the slides.  I have checked
the pH of all the solutions and they are okay.  Any ideas.  The stain is
readable, but does not look too nice.
thanks
Diana