[Histonet] Staining Muscle proteins

[Said Bendahhou]

Hi all,
I'm looking for a protocol to label skeletal muscle compartments (T- 
tubules, SR...) of frozen sections, using mice antibodies. Mice  
muscle was cut and embedded in OCT, frozen using isopentane, and cut  
at 8 um. Cover slips were stored at -20C. Expecting details regarding  
fixation, permeabilization for better access to all compartments.  
Secondary antibodies used are Alexa 488 or 594.
Thanks,
Said