From jkiernan <@t> uwo.ca Mon Oct 1 00:19:25 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Mon Oct 1 00:19:29 2007 Subject: [Histonet] Polymer Paraffin Message-ID: Dear Igor Deyneko, Go for simple paraffin wax that melts between 55C and 60C. Additives, especially unidentified polymers, probably have little value. Read on, and check the Web and Library references. The various paraffins and additives were discussed on Histonet several (8-10) years ago, and the replies included comments from the major authority in this field, Russ Allison of the University of Wales Dental School in Cardiff. I hope this information is still available by way of Histosearch.com, because Russ's original publications in the field are not in many libraries outside Britain and the Commonwealth. Russ Allison is one of the authors of the 4th (last, 1985) edition of C.Culling's excellent textbook, and he published significant original papers on waxes from the mid-1970s up to 1998. He compared many commercial and extempore formulations (more than a hundred, if I remember rightly) using physical measurements of the variation in force resisting the passage of the block face across the blade, scanning EM to see how solidification of the wax deformed fine structure, and determination of the rate of infiltration of tissue, and how this was affected by temperature, MW of the wax, additives etc. His bottom lines were that:\ 1. Additives to paraffin, declared or secret, have no effect on cutting or the appearance of stained sections. This conclusion dismissed many unprovedc claims for additives to paraffin wax. The secret polymer additives (often with short lifespans, according to the salesmen) do not help in any way. 2. Infiltration by paraffin is somewhat quicker if the wax contains some DMSO (= dimethyl sulphoxide, a solvent miscible with nearly everything). All but the most recent of Russ Allison's papers were published in a British journal that has changed its name too many times. Some are in the British Journal of Medical Laboratory Science, and others are in Medical Laboratory Sciences. The volume numbers are continuous, dating from the good old Journal of Medical Laboratory Technology, which contains some real classics in the field of Histotechnology. John A. Kiernan Anatomy & Cell Biology University of Western Ontario London, Canada N6A 5C1 ------ Igor Deyneko wrote: > Dear Histonetters! I was just wondering, can someone > advise a good > embedding > paraffin for me? I embed tumors mostly, and occasional livers > and pancreas, > and very rarely colon. The 1 I'm using right now, Richard-Allen > #6, I'm not > crazy about. I am looking for one preferably with polymers. Has > anyone ever > used the SurgiPath Embedding paraffin and what are your reflections? > Thank you. > Igor Deyneko. > Infinity Pharmaceuticals > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Mon Oct 1 07:07:47 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Mon Oct 1 07:07:55 2007 Subject: [Histonet] Polymer Paraffin Message-ID: <407F05A128805F4C879A33DBA32E618E018950AF@TRFT-EX01.xRothGen.nhs.uk> Yes - but where is Russ? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Kiernan Sent: 01 October 2007 06:19 To: patsy ruegg; Histonet@lists.utsouthwestern.edu Cc: Histonet@lists.utsouthwestern.edu Subject: Re: RE: [Histonet] Polymer Paraffin Dear Igor Deyneko, Go for simple paraffin wax that melts between 55C and 60C. Additives, especially unidentified polymers, probably have little value. Read on, and check the Web and Library references. The various paraffins and additives were discussed on Histonet several (8-10) years ago, and the replies included comments from the major authority in this field, Russ Allison of the University of Wales Dental School in Cardiff. I hope this information is still available by way of Histosearch.com, because Russ's original publications in the field are not in many libraries outside Britain and the Commonwealth. Russ Allison is one of the authors of the 4th (last, 1985) edition of C.Culling's excellent textbook, and he published significant original papers on waxes from the mid-1970s up to 1998. He compared many commercial and extempore formulations (more than a hundred, if I remember rightly) using physical measurements of the variation in force resisting the passage of the block face across the blade, scanning EM to see how solidification of the wax deformed fine structure, and determination of the rate of infiltration of tissue, and how this was affected by temperature, MW of the wax, additives etc. His bottom lines were that:\ 1. Additives to paraffin, declared or secret, have no effect on cutting or the appearance of stained sections. This conclusion dismissed many unprovedc claims for additives to paraffin wax. The secret polymer additives (often with short lifespans, according to the salesmen) do not help in any way. 2. Infiltration by paraffin is somewhat quicker if the wax contains some DMSO (= dimethyl sulphoxide, a solvent miscible with nearly everything). All but the most recent of Russ Allison's papers were published in a British journal that has changed its name too many times. Some are in the British Journal of Medical Laboratory Science, and others are in Medical Laboratory Sciences. The volume numbers are continuous, dating from the good old Journal of Medical Laboratory Technology, which contains some real classics in the field of Histotechnology. John A. Kiernan Anatomy & Cell Biology University of Western Ontario London, Canada N6A 5C1 ------ Igor Deyneko wrote: > Dear Histonetters! I was just wondering, can someone advise a good > embedding paraffin for me? I embed tumors mostly, and occasional > livers and pancreas, and very rarely colon. The 1 I'm using right now, > Richard-Allen #6, I'm not crazy about. I am looking for one preferably > with polymers. Has anyone ever used the SurgiPath Embedding paraffin > and what are your reflections? > Thank you. > Igor Deyneko. > Infinity Pharmaceuticals > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmerriam2003 <@t> yahoo.com Mon Oct 1 07:20:01 2007 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Mon Oct 1 07:20:06 2007 Subject: [Histonet] Polymer Paraffin Message-ID: <561073.67517.qm@web50309.mail.re2.yahoo.com> I have been using the Surgipath paraffins (embedding and infiltrating) for nearly 20 years, and I love them. I have tried other brands, but I always come back to the Suripath 2-paraffin system. Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA ----- Original Message ---- From: patsy ruegg To: Rene J Buesa ; Igor Deyneko ; Histonet@lists.utsouthwestern.edu Sent: Sunday, September 30, 2007 1:57:05 PM Subject: RE: [Histonet] Polymer Paraffin I agree I use paraplast plus which I now get from Stat Labs, it is a McCormick brand. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, September 28, 2007 3:51 PM To: Igor Deyneko; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Polymer Paraffin Or infiltrating/embedding paraffin was Paraplast-Plus, and we used with very good results. Ren? j. Igor Deyneko wrote: Dear Histonetters! I was just wondering, can someone advise a good embedding paraffin for me? I embed tumors mostly, and occasional livers and pancreas, and very rarely colon. The 1 I'm using right now, Richard-Allen #6, I'm not crazy about. I am looking for one preferably with polymers. Has anyone ever used the SurgiPath Embedding paraffin and what are your reflections? Thank you. Igor Deyneko. Infinity Pharmaceuticals _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Check out the hottest 2008 models today at Yahoo! Autos. http://autos.yahoo.com/new_cars.html From b-frederick <@t> northwestern.edu Mon Oct 1 08:08:16 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon Oct 1 08:45:26 2007 Subject: [Histonet] Polymer Paraffin In-Reply-To: <561073.67517.qm@web50309.mail.re2.yahoo.com> Message-ID: <007a01c8042c$24563240$d00f7ca5@lurie.northwestern.edu> To all, I've been a paraplast user in my 20 years. Frst plus (on a technicon!!!) and now Extra. The old standard I guess. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Monday, October 01, 2007 7:20 AM To: patsy ruegg; Rene J Buesa; Igor Deyneko; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Polymer Paraffin I have been using the Surgipath paraffins (embedding and infiltrating) for nearly 20 years, and I love them. I have tried other brands, but I always come back to the Suripath 2-paraffin system. Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA ----- Original Message ---- From: patsy ruegg To: Rene J Buesa ; Igor Deyneko ; Histonet@lists.utsouthwestern.edu Sent: Sunday, September 30, 2007 1:57:05 PM Subject: RE: [Histonet] Polymer Paraffin I agree I use paraplast plus which I now get from Stat Labs, it is a McCormick brand. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, September 28, 2007 3:51 PM To: Igor Deyneko; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Polymer Paraffin Or infiltrating/embedding paraffin was Paraplast-Plus, and we used with very good results. Ren? j. Igor Deyneko wrote: Dear Histonetters! I was just wondering, can someone advise a good embedding paraffin for me? I embed tumors mostly, and occasional livers and pancreas, and very rarely colon. The 1 I'm using right now, Richard-Allen #6, I'm not crazy about. I am looking for one preferably with polymers. Has anyone ever used the SurgiPath Embedding paraffin and what are your reflections? Thank you. Igor Deyneko. Infinity Pharmaceuticals _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________ ________ Check out the hottest 2008 models today at Yahoo! Autos. http://autos.yahoo.com/new_cars.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lynn.Burton <@t> Illinois.gov Mon Oct 1 09:06:04 2007 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Mon Oct 1 10:06:46 2007 Subject: [Histonet] Polymer Paraffin References: <35e16a770709281447g2b817dc5w77f6b7664776e873@mail.gmail.com> Message-ID: I use Surgipath EM400 with very good results. We do a lot of tumors and all types of animal organs with it. Lynn Burton Animal Disease Lab Galesburg,Il ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Igor Deyneko Sent: Fri 9/28/2007 4:47 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Polymer Paraffin Dear Histonetters! I was just wondering, can someone advise a good embedding paraffin for me? I embed tumors mostly, and occasional livers and pancreas, and very rarely colon. The 1 I'm using right now, Richard-Allen #6, I'm not crazy about. I am looking for one preferably with polymers. Has anyone ever used the SurgiPath Embedding paraffin and what are your reflections? Thank you. Igor Deyneko. Infinity Pharmaceuticals _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Mon Oct 1 09:21:51 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Mon Oct 1 10:22:04 2007 Subject: [Histonet] Polymer Paraffin In-Reply-To: <007a01c8042c$24563240$d00f7ca5@lurie.northwestern.edu> References: <561073.67517.qm@web50309.mail.re2.yahoo.com> <007a01c8042c$24563240$d00f7ca5@lurie.northwestern.edu> Message-ID: Paraplast plus here, as well. I didn't know there were other brands :) Emily -- Yog-Sothoth knows the gate. Yog-Sothoth is the gate. Yog-Sothoth is the key and guardian of the gate. Past, present, future, all are one in Yog-Sothoth. He knows where the Old Ones broke through of old, and where They shall break through again. From amgomez <@t> mail.unomaha.edu Mon Oct 1 09:26:12 2007 From: amgomez <@t> mail.unomaha.edu (Adam M Gomez) Date: Mon Oct 1 10:25:46 2007 Subject: [Histonet] CD 138 In-Reply-To: Message-ID: Please remove me from your distro box. V/R Adam Gomez NCOIC, Personnel AFROTC Det 470 Comm: 402-554-3402 Fax: 402-554-2999 University of Nebraska at Omaha amgomez@mail.unomaha.edu Helen E Johnson Sent by: histonet-bounces@lists.utsouthwestern.edu 09/26/2007 03:50 PM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] CD 138 Hi Histonetters, Does anyone have an IHC protocol for CD 138 [Rat anti-mouse] [Unconjugated] for FFPE mouse spleen? Helen Johnson (hej01@health.state.ny.us) IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Mon Oct 1 10:02:28 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Mon Oct 1 10:42:34 2007 Subject: [Histonet] Reference lab doing IHC on cytospins In-Reply-To: References: Message-ID: <4700D3C402000077000084A1@gwmail4.harthosp.org> Yes, we do a lot of ICC on cytology smears, Thin-preps, and Paps. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Sebree Linda A." 09/28/07 5:09 PM >>> Hello 'netters: We're wondering if there's a reference lab out there that does immunohistochemistry testing on cytology preparations. Have a good weekend all! Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From jkiernan <@t> uwo.ca Mon Oct 1 10:34:17 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Mon Oct 1 11:09:50 2007 Subject: [Histonet] Polymer Paraffin In-Reply-To: References: <561073.67517.qm@web50309.mail.re2.yahoo.com> <007a01c8042c$24563240$d00f7ca5@lurie.northwestern.edu> Message-ID: The most recent of Russ Allison's internet statements on paraffins may be an FAQ answer: http://publish.uwo.ca/~jkiernan/faqlist.htm#WAXCRY John Kiernan London, Canada. --- From pmarcum <@t> vet.upenn.edu Mon Oct 1 10:48:56 2007 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Mon Oct 1 11:16:38 2007 Subject: [Histonet] Polymer Paraffin In-Reply-To: References: <561073.67517.qm@web50309.mail.re2.yahoo.com> <007a01c8042c$24563240$d00f7ca5@lurie.northwestern.edu> Message-ID: <6.2.5.6.2.20071001114248.01bf7120@vet.upenn.edu> At 10:21 AM 10/1/2007, Emily Sours wrote: >Paraplast plus here, as well. >I didn't know there were other brands :) > >Emily >-- >Yog-Sothoth knows the gate. >Yog-Sothoth is the gate. >Yog-Sothoth is the key and guardian of the gate. Past, present, future, all >are one in Yog-Sothoth. >He knows where the Old Ones broke through of old, and where They shall break >through again. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Paraffin is really a laboratory choice of what works best in your facility. I have tested and worked with most paraffins on the market and all have some level of polymer in them. Numerous reasons are given for why one is better than the other. I have found difference in the paraffins based of what I needed at the time and changes as needed. I don't do infiltration paraffins and embedding as I don't like the concept personally and it was really based on tissue processor problems caused when too much polymer caused valves to clog years ago. The best paraffins are simply the one that works for you. Currently we use Polyscientific Paraffin Prills and really like them. I have used all versions of Paraplast, some Richard Allan, Surgipath, Polysciences and others with some differences but all usable. As someone who has worked on paraffin development for several companies the differences are not as great as they would have us believe. Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From sheila_adey <@t> hotmail.com Mon Oct 1 10:56:02 2007 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Mon Oct 1 11:20:48 2007 Subject: [Histonet] Neutralization of Formalin In-Reply-To: <45109da50709291553rbc67d6aw97c7f1a339cc2317@mail.gmail.com> References: <45109da50709281029n3fef9adbmfeaaa25c2565d781@mail.gmail.com> <45109da50709281654l6298a7feu31e4b56824024769@mail.gmail.com> <45109da50709281702h16bc77a9g51eb5eb29c28b840@mail.gmail.com> <45109da50709291553rbc67d6aw97c7f1a339cc2317@mail.gmail.com> Message-ID: We use Neutralex from Sakura and then dump down the drain. They also sell the test strips.Sheila Adey HT MLTPort Huron HospitalMichigan> Date: Sat, 29 Sep 2007 14:53:57 -0800> From: bob.nienhuis@gmail.com> To: histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Neutralization of Formalin> CC: nienhuis@ucla.edu> > Oops. I guess I can't attach files to Histonet messages.> > I have uploaded a copy of the Calif. EPA Formalex de-certification memo to:> > > Hope this works.> > Bob> UCLA / VA Medical Center> Sepulveda, CA> > On 9/28/07, Bob Nienhuis wrote:> >> >> >> > See attached memo from the California State EPA re: Notice of Intent to> > Deny Re-Certification> > Hazardous Waste Environmental Technology.> >> > Bob> > UCLA / VA Medical Center> > Sepulveda, CA> >> > On 9/28/07, Rathborne, Toni wrote:> > >> > > Bob,> > >> > > Please explain. We use Formalex too.> > >> > > Thanks,> > > Toni Rathborne> > > Pathology Supervisor> > > Somerset Medical Center> > > 110 Rehill Ave.> > > Somerville,NJ 08876> > > 908-595-2367> > >> > >> > > -----Original Message-----> > > From: histonet-bounces@lists.utsouthwestern.edu> > > [mailto: histonet-bounces@lists.utsouthwestern.edu ]On Behalf Of Bob> > > Nienhuis> > > Sent: Friday, September 28, 2007 1:30 PM> > > To: Schaundra Walton> > > Cc: Histonet> > > Subject: Re: [Histonet] Neutralization of Formalin> > >> > >> > > We used to do that, but are not allowed to do it anymore. Apparently,> > > the> > > Formulex produces some> > > stuff that is nastier than the original formalin, We send to a waste> > > disposal company.> > >> > > Bob> > > UCLA / VA Medical Center> > > Sepulveda, CA> > >> > > On 9/28/07, Schaundra Walton < schaundrawalton@yahoo.com> wrote:> > > >> > > > Hi everyone!> > > >> > > > Our lab neutralizes our 10% formalin before dumping it down the> > > > drain. We use Formalex (American Master Tech) to neutralize and then> > > add> > > > sodium hydroxide to balance the pH. We had been using residual> > > formaldehyde> > > > test strips to test the ppm (Market Lab) before dumping, but they have> > > been> > > > discontinued. We have tried to replace them with EM formaldehyde test> > >> > > > strips (Fisher Scientific), but we are having trouble getting them to> > > > work. Anyone else neutralizing their formalin? What are you using> > > and are> > > > you testing it before dumping it?> > > >> > > >> > > > Schaundra Walton BS HTL(ASCP)> > > > Swedish American Hospital> > > > 1401 E. State St.> > > > Rockford, IL 61104> > > >> > > > ---------------------------------> > > > Take the Internet to Go: Yahoo!Go puts the Internet in your pocket:> > > mail,> > > > news, photos & more.> > > > _______________________________________________> > > > Histonet mailing list> > > > Histonet@lists.utsouthwestern.edu> > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > >> > > _______________________________________________> > > Histonet mailing list> > > Histonet@lists.utsouthwestern.edu> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > >> > >> > >> >> >> >> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Connect to the next generation of MSN Messenger? http://imagine-msn.com/messenger/launch80/default.aspx?locale=en-us&source=wlmailtagline From ckeith71 <@t> hotmail.com Mon Oct 1 11:06:59 2007 From: ckeith71 <@t> hotmail.com (cindy keith) Date: Mon Oct 1 11:28:55 2007 Subject: [Histonet] Recyclers Message-ID: Anyone have any recommendations for recyclers? _________________________________________________________________ Invite your mail contacts to join your friends list with Windows Live Spaces. It's easy! http://spaces.live.com/spacesapi.aspx?wx_action=create&wx_url=/friends.aspx&mkt=en-us From rjbuesa <@t> yahoo.com Mon Oct 1 11:37:15 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Oct 1 11:39:32 2007 Subject: [Histonet] Recyclers In-Reply-To: Message-ID: <84021.91186.qm@web61224.mail.yahoo.com> I used during years a B/R Corp. recycler for xylene. It was a "work horse" that paid itself in less than 2 years. Ren? J. cindy keith wrote: Anyone have any recommendations for recyclers? _________________________________________________________________ Invite your mail contacts to join your friends list with Windows Live Spaces. It's easy! http://spaces.live.com/spacesapi.aspx?wx_action=create&wx_url=/friends.aspx&mkt=en-us_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Tonight's top picks. What will you watch tonight? Preview the hottest shows on Yahoo! TV. From vazquezr <@t> ohsu.edu Mon Oct 1 12:00:13 2007 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Mon Oct 1 12:04:40 2007 Subject: [Histonet] Recyclers Message-ID: Of what??? >>> "cindy keith" 10/1/2007 9:06 AM >>> Anyone have any recommendations for recyclers? _________________________________________________________________ Invite your mail contacts to join your friends list with Windows Live Spaces. It's easy! http://spaces.live.com/spacesapi.aspx?wx_action=create&wx_url=/friends.aspx&mkt=en-us_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tim.morken <@t> thermofisher.com Mon Oct 1 12:04:36 2007 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Mon Oct 1 12:30:08 2007 Subject: [Histonet] Polymer Paraffin In-Reply-To: References: <561073.67517.qm@web50309.mail.re2.yahoo.com><007a01c8042c$24563240$d00f7ca5@lurie.northwestern.edu> Message-ID: <6BFF6D137DF6BC43B33891BA96E83B19CA695D@PGHCR-EXMB-VS-1.na.fshrnet.com> Russ has a good idea in going to "pure" paraffin, that is, without additives, in order to see how non-additive paraffin works. Then comparing to paraffin with additives may give an idea on what you need. However, it is almost impossible to find out exactly what is being added to these paraffins. Most manufactures are adding "resins" of some kind. These are usually in the class of "thermoplastic" resins - those only plastic at high temperatures (vinyls, styrenes, butyls, acrylates, urethanes). As such, they are not plastic at room temperature. Indeed, most of these resins are the same as those found in hot-melt glue, which should give you an idea on how they will act. Some, if exposed to high heat, may polymerize in a way that makes it impossible to remove them from a section or slide. This may be why there is sometimes a "background" of stain around a tissue that matches the area of the original wax area of the section. Most of these add strength to the paraffin, but some make it more "sticky" so it "ribbons" better. These are added in percentages of 5 - 15% from what I can glean from their literature. Tim Morken Lab Vison Products ThermoFisher Scientific -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Kiernan Sent: Monday, October 01, 2007 8:34 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Polymer Paraffin The most recent of Russ Allison's internet statements on paraffins may be an FAQ answer: http://publish.uwo.ca/~jkiernan/faqlist.htm#WAXCRY John Kiernan London, Canada. From Robert.Lott <@t> TriadHospitals.com Mon Oct 1 12:23:05 2007 From: Robert.Lott <@t> TriadHospitals.com (Lott, Robert) Date: Mon Oct 1 12:33:47 2007 Subject: [Histonet] IHC and Cytology (IHC Forum) Message-ID: <518A08C53ED96D419F498D309E64A36A2E7B20@CPRTEVS03.triadhospitals.net> I just had to weigh in on this... Most of you are familiar with Dr. Rodney Miller. He is the Director of Immunohistochemistry at ProPath Labs in Dallas. He is world renown for his expertise. He is also a board certified cytopathologist and a true friend to NSH having spoken at many local, state, and national meetings. Dr. Miller wrote a book :-) called Practical Cytopathology. The COMPLETE contents of this textbook are as follows: Get a good cell block and do immunostains if you can't figure it out on the H&E. THE END For more information follow this link: http://www.propathlab.com/pdf/2005-01_cytology.pdf Robert Robert L. Lott, HTL(ASCP) Manager, Anatomic Pathology Trinity Medical Center / LabFirst 800 Montclair Road Birmingham, AL 35213 205-592-5388 205-592-5646 - fax robert.lott@triadhospitals.com Date: Sun, 30 Sep 2007 11:03:18 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] IHC Forum To: patsy ruegg , Histonet@pathology.swmed.edu Message-ID: <513570.38831.qm@web61211.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi Patsy: This week there was a discussion in Histonet about IHC on PAP smears and cytospins and using tissue sections as positive controls or not. Roughly the positions were the following: 1- some accepted using FFPE tissue sections as (+) controls, after HIER 2- some suggested fixing the smears with NBF and cause the "crossslinkage" to do HIER afterwards 3- some advocated using ONLY known (+) smears or frozen sections as (+) controls, i.e. non-fixed tissues. The rational being a CAP instruction in which they direct to use controls treated the same as the case. I think this is an important topic but I also think the "discussion" should be beyond an intelectual argument of each, because that will just expand what has been said this last week. I think that with some time ahead some "hard evidence" ought to be presented for discussion, i.e. results obtained using the 3 "approaches" summarized above. The panel then should view the slides, grade them and get to a determination. The slides should be reviewed/graded "blind", i.e. no one shoudl know the procedure s/he is evaluating. Give consideration to this proposal. Ren? J. patsy ruegg wrote: This is a call for IHC questions to be discussed at the NSH S/C in Denver in OCT. If you have any burning questions you would like our panel of 9 to address please email them to me ahead of time. Thank you, Patsy Ruegg From Heather.D.Renko <@t> osfhealthcare.org Mon Oct 1 13:01:35 2007 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Mon Oct 1 13:02:13 2007 Subject: [Histonet] Copath Users/Cassette labeler interfacing Message-ID: <40026EDDE64CDA47AB382C52619ACD3C0A09DB17@pmc-rfd-mx01.intranet.osfnet.org> I was hoping to get some Copath Plus users out there that have cassette printers that are interfaced to email me. Thank you in advance. Heather Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From doug <@t> ppspath.com Mon Oct 1 14:41:14 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Mon Oct 1 13:43:12 2007 Subject: [Histonet] Manual Leitz 1720 Message-ID: I am looking for a manual for a Leitz 1720 cryostat. Thanks! Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. From rjbuesa <@t> yahoo.com Mon Oct 1 14:42:29 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Oct 1 14:42:33 2007 Subject: [Histonet] IHC and Cytology (IHC Forum) In-Reply-To: <518A08C53ED96D419F498D309E64A36A2E7B20@CPRTEVS03.triadhospitals.net> Message-ID: <224172.56409.qm@web61224.mail.yahoo.com> But that is not what is behind last week's discussion, which dealt with PAP smears using FFPE tissue sectoins used as (+) controls. What Dr. Miller says is similar to what Dr. Rosai also says: the H&E should be the "gold standard" and the IHC is a way of either trying to figure out some obscure origin or differentiating between several possibilities. Also if dealing with a cell block the discussion is unnecessary, since cell blocks are FFPE also. Ren? J. "Lott, Robert" wrote: I just had to weigh in on this... Most of you are familiar with Dr. Rodney Miller. He is the Director of Immunohistochemistry at ProPath Labs in Dallas. He is world renown for his expertise. He is also a board certified cytopathologist and a true friend to NSH having spoken at many local, state, and national meetings. Dr. Miller wrote a book :-) called Practical Cytopathology. The COMPLETE contents of this textbook are as follows: Get a good cell block and do immunostains if you can't figure it out on the H&E. THE END For more information follow this link: http://www.propathlab.com/pdf/2005-01_cytology.pdf Robert Robert L. Lott, HTL(ASCP) Manager, Anatomic Pathology Trinity Medical Center / LabFirst 800 Montclair Road Birmingham, AL 35213 205-592-5388 205-592-5646 - fax robert.lott@triadhospitals.com Date: Sun, 30 Sep 2007 11:03:18 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] IHC Forum To: patsy ruegg , Histonet@pathology.swmed.edu Message-ID: <513570.38831.qm@web61211.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi Patsy: This week there was a discussion in Histonet about IHC on PAP smears and cytospins and using tissue sections as positive controls or not. Roughly the positions were the following: 1- some accepted using FFPE tissue sections as (+) controls, after HIER 2- some suggested fixing the smears with NBF and cause the "crossslinkage" to do HIER afterwards 3- some advocated using ONLY known (+) smears or frozen sections as (+) controls, i.e. non-fixed tissues. The rational being a CAP instruction in which they direct to use controls treated the same as the case. I think this is an important topic but I also think the "discussion" should be beyond an intelectual argument of each, because that will just expand what has been said this last week. I think that with some time ahead some "hard evidence" ought to be presented for discussion, i.e. results obtained using the 3 "approaches" summarized above. The panel then should view the slides, grade them and get to a determination. The slides should be reviewed/graded "blind", i.e. no one shoudl know the procedure s/he is evaluating. Give consideration to this proposal. Ren? J. patsy ruegg wrote: This is a call for IHC questions to be discussed at the NSH S/C in Denver in OCT. If you have any burning questions you would like our panel of 9 to address please email them to me ahead of time. Thank you, Patsy Ruegg _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Need a vacation? Get great deals to amazing places on Yahoo! Travel. From mari.ann.mailhiot <@t> leica-microsystems.com Mon Oct 1 14:59:29 2007 From: mari.ann.mailhiot <@t> leica-microsystems.com (mari.ann.mailhiot@leica-microsystems.com) Date: Mon Oct 1 15:01:31 2007 Subject: [Histonet] Manual Leitz 1720 In-Reply-To: <20071001184437.AC792D00F878@de-barracuda.leica-microsystems.com> Message-ID: Hi Douglas Please contact me by email and I can arrange for a copy of the instruction manual. Best regards Mari Ann Mailhiot BA HT ASCP Application Specialist Leica Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com "Douglas D Deltour" Sent by: cc histonet-bounces@ lists.utsouthwest Subject ern.edu [Histonet] Manual Leitz 1720 10/01/2007 02:41 PM I am looking for a manual for a Leitz 1720 cryostat. Thanks! Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From eschurga <@t> aerotek.com Mon Oct 1 16:58:46 2007 From: eschurga <@t> aerotek.com (Schurga, Erich) Date: Mon Oct 1 16:58:53 2007 Subject: [Histonet] Histotechnologist Positions in Central Florida Message-ID: Good Evening, I'm looking for five Histotechnologist for an established lab in Central Florida. Attached below is the job description. Starting pay is between $26/hr - $34/hr, based on experience. Please contact me directly if you would like more information, or if you know of any Histotechnologists looking for work. My client is looking to hire immediately and these are all direct placement positions. Histotechnologist Job Description: An established Laboratory in Altamonte Springs, FL is currently seeking five Histotechnologist, entry level to experienced. This is a direct placement job, with a salary range of $26 - $34/hr. based on experience. We offer competitive salaries; health, dental and disability insurance; paid holidays; paid vacations; and 401(k) retirement plan. Responsibilities consist of all elements of biopsy processing and staining for diagnosis. Precisely and accurately performs a variety of routine and specialized histology techniques and procedures. Meets embedding and sectioning productivity standards. Qualified Histotechnologists will have: - Training and certification as HT or HLT by ASCP - Florida Licensure - AS from accredited school in Histology or related science. Erich J. Schurga Scientific Recruiter Aerotek, Inc. * Phone: 321.354.1057 * Fax: 321.354.1080 * Toll Free: 888.455.1329 * E-mail: eschurga@aerotek.com 3660 Maguire Blvd. Suite 107 Orlando, FL 32803-3059 Click here to search for open positions: www.aerotek.com ____________________________________________________________________________________________________ This electronic mail (including any attachments) may contain information that is privileged, confidential, and/or otherwise protected from disclosure to anyone other than its intended recipient(s). Any dissemination or use of this electronic email or its contents (including any attachments) by persons other than the intended recipient(s) is strictly prohibited. If you have received this message in error, please notify us immediately by reply email so that we may correct our internal records. Please then delete the original message (including any attachments) in its entirety. Thank you. From spirowg <@t> aol.com Tue Oct 2 00:32:19 2007 From: spirowg <@t> aol.com (spirowg@aol.com) Date: Tue Oct 2 00:32:25 2007 Subject: [Histonet] Immunofluorescence Message-ID: <8C9D2CCBC2E54A3-1474-3DE0@webmail-da03.sysops.aol.com> Hello. Our lab is receiving many request for immunofluorescence especially for skins and kidneys.? Does this occur in your lab if so why? Thanks ________________________________________________________________________ Email and AIM finally together. You've gotta check out free AOL Mail! - http://mail.aol.com From louise.renton <@t> gmail.com Tue Oct 2 03:33:49 2007 From: louise.renton <@t> gmail.com (louise renton) Date: Tue Oct 2 03:34:02 2007 Subject: [Histonet] bioceramic & Technovit 7200 Message-ID: HELP!! I need to grind sections of a porous bioceramic implant (no tissue attached). If I want to embed in Technovit & do EXAKT sections..with which solution do I start? a 50:50 alcohol/resin mix, or jsut straight into the monomer for a couple of days? I would appreciate soem info, as I only have 1 sample to get this right first time on(as always) best regards -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From rjbuesa <@t> yahoo.com Tue Oct 2 07:21:35 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Oct 2 07:21:46 2007 Subject: [Histonet] Immunofluorescence In-Reply-To: <8C9D2CCBC2E54A3-1474-3DE0@webmail-da03.sysops.aol.com> Message-ID: <226561.99975.qm@web61212.mail.yahoo.com> That used to happen to us also when our lab signed new dermatologists accounts that attended a large population of elderly patients. Maybe something similar is happening to you now also. Ren? J. spirowg@aol.com wrote: Hello. Our lab is receiving many request for immunofluorescence especially for skins and kidneys.? Does this occur in your lab if so why? Thanks ________________________________________________________________________ Email and AIM finally together. You've gotta check out free AOL Mail! - http://mail.aol.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Need a vacation? Get great deals to amazing places on Yahoo! Travel. From PMcArdle <@t> ebsciences.com Tue Oct 2 07:45:54 2007 From: PMcArdle <@t> ebsciences.com (Phil McArdle) Date: Tue Oct 2 07:46:08 2007 Subject: [Histonet] bioceramic & Technovit 7200 In-Reply-To: References: Message-ID: <47023D82.3080800@ebsciences.com> Hello: Although you are in South Africa, we are Heraeus Kulzer's US distributor, and I'm only familiar with the 3040, 7100, 8100 and 9100 products we carry (not 7200), I will forward your query to Heraeus' tech support in Germany. Hope this helps! Best regards, Phil McArdle -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Mobile: 860.597.6796 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com I skate to where the puck is going to be, not to where it's been. - Wayne Gretsky You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it, and it was probably my mistake anyway. So please do the right thing and make this e-mail go away. Thank you. louise renton wrote: > HELP!! I need to grind sections of a porous bioceramic implant (no tissue > attached). If I want to embed in Technovit & do EXAKT sections..with which > solution do I start? a 50:50 alcohol/resin mix, or jsut straight into the > monomer for a couple of days? > > I would appreciate soem info, as I only have 1 sample to get this right > first time on(as always) > > best regards > From pex0220 <@t> yahoo.com.cn Tue Oct 2 09:27:39 2007 From: pex0220 <@t> yahoo.com.cn (docqian) Date: Tue Oct 2 09:27:57 2007 Subject: [Histonet] Runx2 antibody Message-ID: <190653.22775.qm@web15215.mail.cnb.yahoo.com> Dear all,=0A=0ADoes anyone know which company sells a good anti-Runx2 antib= ody? Or do some labs produce this antibody by themselves? =0A=0AThank you.= =0A =0AGuofeng=0A=0A=0A ______________________________________________= _____________ =0A=D1=C5=BB=A2=D3=CA=CF=E4=A3=AC=D6=D5=C9=FA=BB=EF=B0=E9=A3= =A1 =0Ahttp://mail.yahoo.com.cn/From amgomez <@t> mail.unomaha.edu Tue Oct 2 10:02:29 2007 From: amgomez <@t> mail.unomaha.edu (Adam M Gomez) Date: Tue Oct 2 10:02:52 2007 Subject: [Histonet] Runx2 antibody In-Reply-To: <190653.22775.qm@web15215.mail.cnb.yahoo.com> Message-ID: UGxlYXNlIHJlbW92ZSBtZSBmcm9tIHlvdXIgZGlzdHJvIGJveCBvciBlbWFpbHMuDQoNCg0KQWRh bSBHb21leg0KTkNPSUMsIFBlcnNvbm5lbCANCkFGUk9UQyBEZXQgNDcwDQpDb21tOiA0MDItNTU0 LTM0MDIgRmF4OiA0MDItNTU0LTI5OTkNClVuaXZlcnNpdHkgb2YgTmVicmFza2EgYXQgT21haGEN CmFtZ29tZXpAbWFpbC51bm9tYWhhLmVkdQ0KDQoNCg0KZG9jcWlhbiA8cGV4MDIyMEB5YWhvby5j b20uY24+IA0KU2VudCBieTogaGlzdG9uZXQtYm91bmNlc0BsaXN0cy51dHNvdXRod2VzdGVybi5l ZHUNCjEwLzAyLzIwMDcgMDk6MzYgQU0NCg0KVG8NCkhpc3RvbmV0QGxpc3RzLnV0c291dGh3ZXN0 ZXJuLmVkdQ0KY2MNCg0KU3ViamVjdA0KW0hpc3RvbmV0XSBSdW54MiBhbnRpYm9keQ0KDQoNCg0K DQoNCg0KRGVhciBhbGwsDQoNCkRvZXMgYW55b25lIGtub3cgd2hpY2ggY29tcGFueSBzZWxscyBh IGdvb2QgYW50aS1SdW54MiBhbnRpYm9keT8gT3IgZG8gDQpzb21lIGxhYnMgcHJvZHVjZSB0aGlz IGFudGlib2R5IGJ5IHRoZW1zZWx2ZXM/IA0KDQpUaGFuayB5b3UuDQogDQpHdW9mZW5nDQoNCg0K ICAgICAgX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19f X19fX19fX18gDQrRxbui08rP5KOs1tXJ+rvvsOmjoSANCmh0dHA6Ly9tYWlsLnlhaG9vLmNvbS5j bi8NCl9fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fDQpIaXN0 b25ldCBtYWlsaW5nIGxpc3QNCkhpc3RvbmV0QGxpc3RzLnV0c291dGh3ZXN0ZXJuLmVkdQ0KaHR0 cDovL2xpc3RzLnV0c291dGh3ZXN0ZXJuLmVkdS9tYWlsbWFuL2xpc3RpbmZvL2hpc3RvbmV0DQoN Cg0KFrom settembr <@t> umdnj.edu Tue Oct 2 10:57:18 2007 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Tue Oct 2 10:57:57 2007 Subject: [Histonet] Mammaglobin A & GCDFP-15 Message-ID: Hello Brett, I cannot help you with Mam A but I do have a supplier for GCDFP-15. I use Signet Pathlogy Systems in Dedham, Mass. 1-800-223-0796 I use their GCDFP-15 (Brst-2) clone D6 on human tissue. It is made in mouse. We rarely use it so I buy their prediluted 6ml vial. I dilute that 1:1. I use Dako's Ready to Use Prot. K for 5 minutes @ RT. Hope this helps. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> "Connolly, Brett M" 09/28/07 4:00 PM >>> Hi all, Looking for suppliers of these antibodies for FFPE tissues. Also, necessary pretreatments/dilutions used. Thanks, Brett Brett M. Connolly, Ph.D. Research Fellow MRL, Imaging Research Merck & Co., Inc. WP-44K PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com ------------------------------------------------------------------------------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Tue Oct 2 16:11:37 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Oct 2 16:11:57 2007 Subject: [Histonet] IHC and Cytology (IHC Forum) In-Reply-To: <518A08C53ED96D419F498D309E64A36A2E7B20@CPRTEVS03.triadhospitals.net> References: <518A08C53ED96D419F498D309E64A36A2E7B20@CPRTEVS03.triadhospitals.net> Message-ID: <47027BC90200007700008534@gwmail4.harthosp.org> I agree that cell blocks are most helpful, but, the simple truth is, "they are not always available". We do immunocytochemistry on cytology smears, Thin-Preps, and Paps almost every day and we get very good results with most antibodies. I have performed validations for most of our antibodies so I know which ones work on alcohol-fixed cells and which ones do not. I run paraffin sections for positive controls because it's not practical to prepare cytology smears for this purpose and I hardly ever run a negative control. I don't think a negative control is that important especially when you are using polymer detection. When possible, it is important to identify "internal" positive and negative controls within the test slide. We also use heat-induced antigen retrieval for many antibodies because it is essential even though the cells are not fixed in formalin. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Lott, Robert" 10/01/07 1:23 PM >>> I just had to weigh in on this... Most of you are familiar with Dr. Rodney Miller. He is the Director of Immunohistochemistry at ProPath Labs in Dallas. He is world renown for his expertise. He is also a board certified cytopathologist and a true friend to NSH having spoken at many local, state, and national meetings. Dr. Miller wrote a book :-) called Practical Cytopathology. The COMPLETE contents of this textbook are as follows: Get a good cell block and do immunostains if you can't figure it out on the H&E. THE END For more information follow this link: http://www.propathlab.com/pdf/2005-01_cytology.pdf Robert Robert L. Lott, HTL(ASCP) Manager, Anatomic Pathology Trinity Medical Center / LabFirst 800 Montclair Road Birmingham, AL 35213 205-592-5388 205-592-5646 - fax robert.lott@triadhospitals.com Date: Sun, 30 Sep 2007 11:03:18 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] IHC Forum To: patsy ruegg , Histonet@pathology.swmed.edu Message-ID: <513570.38831.qm@web61211.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi Patsy: This week there was a discussion in Histonet about IHC on PAP smears and cytospins and using tissue sections as positive controls or not. Roughly the positions were the following: 1- some accepted using FFPE tissue sections as (+) controls, after HIER 2- some suggested fixing the smears with NBF and cause the "crossslinkage" to do HIER afterwards 3- some advocated using ONLY known (+) smears or frozen sections as (+) controls, i.e. non-fixed tissues. The rational being a CAP instruction in which they direct to use controls treated the same as the case. I think this is an important topic but I also think the "discussion" should be beyond an intelectual argument of each, because that will just expand what has been said this last week. I think that with some time ahead some "hard evidence" ought to be presented for discussion, i.e. results obtained using the 3 "approaches" summarized above. The panel then should view the slides, grade them and get to a determination. The slides should be reviewed/graded "blind", i.e. no one shoudl know the procedure s/he is evaluating. Give consideration to this proposal. Ren? J. patsy ruegg wrote: This is a call for IHC questions to be discussed at the NSH S/C in Denver in OCT. If you have any burning questions you would like our panel of 9 to address please email them to me ahead of time. Thank you, Patsy Ruegg _____________________________ __________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From trinimaican2501 <@t> yahoo.com Tue Oct 2 16:57:52 2007 From: trinimaican2501 <@t> yahoo.com (I-sanna Gibbons) Date: Tue Oct 2 16:57:57 2007 Subject: [Histonet] overinfiltration of brain tissue? Message-ID: <778765.95801.qm@web50310.mail.re2.yahoo.com> Hi all, If brain tissue is placed in a vacuum for wax infiltration for too long a time, what problems can arise? Can this lead to sectioning problems? How can the effects be reversed? Thanks I-sanna ____________________________________________________________________________________ Catch up on fall's hot new shows on Yahoo! TV. Watch previews, get listings, and more! http://tv.yahoo.com/collections/3658 From Kemlo.Rogerson <@t> waht.swest.nhs.uk Wed Oct 3 02:22:34 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Oct 3 02:22:46 2007 Subject: [Histonet] overinfiltration of brain tissue? Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222EE23@wahtntex2.waht.swest.nhs.uk> Hi all, If brain tissue is placed in a vacuum for wax infiltration for too long a time, what problems can arise? Can this lead to sectioning problems? How can the effects be reversed? Thanks I-sanna If you leave tissue in wax for too long then the heat makes it go hard; this is especially so with brain tissue which can go so hard as to be nearly impossible to section. There are some 'recovery' fluids that include oil of cedar wood but they only mitigate the problem a small amount. I'm afraid brain is one of those organs that needs 'special' processing. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Giving is more a dictate of the heart than a command of the brain. --Henry A. Russo This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Susan.Walzer <@t> HCAHealthcare.com Wed Oct 3 03:28:05 2007 From: Susan.Walzer <@t> HCAHealthcare.com (Walzer Susan) Date: Wed Oct 3 03:28:11 2007 Subject: [Histonet] Recyclers In-Reply-To: Message-ID: <471953BC63077941B82C26A4338272B42F0540@ORLEV03.hca.corpad.net> We use CBG for xylene, formalin and alcohol. It does a great job, few problems, easy to use. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of cindy keith Sent: Monday, October 01, 2007 12:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recyclers Anyone have any recommendations for recyclers? _________________________________________________________________ Invite your mail contacts to join your friends list with Windows Live Spaces. It's easy! http://spaces.live.com/spacesapi.aspx?wx_action=create&wx_url=/friends.aspx&mkt=en-us_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Wed Oct 3 03:49:46 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Wed Oct 3 03:49:52 2007 Subject: Fwd: [Histonet] Recyclers In-Reply-To: References: <471953BC63077941B82C26A4338272B42F0540@ORLEV03.hca.corpad.net> Message-ID: On 10/3/07, Emily Sours wrote: > > do labs use recycling of xylene/alcohol to save money on buying new > alcohol/xylene. or to help the environment, or to save money on > shipping/storing/waste? > since we don't use that much xylene or alchohol, i was wondering how other > labs are affected by recycling. > > -- > Yog-Sothoth knows the gate. > Yog-Sothoth is the gate. > Yog-Sothoth is the key and guardian of the gate. Past, present, future, > all are one in Yog-Sothoth. > He knows where the Old Ones broke through of old, and where They shall > break through again. From rjbuesa <@t> yahoo.com Wed Oct 3 07:24:13 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Oct 3 07:24:19 2007 Subject: Fwd: [Histonet] Recyclers In-Reply-To: Message-ID: <466158.86960.qm@web61212.mail.yahoo.com> All of the above! Ren? J. Emily Sours wrote: On 10/3/07, Emily Sours wrote: > > do labs use recycling of xylene/alcohol to save money on buying new > alcohol/xylene. or to help the environment, or to save money on > shipping/storing/waste? > since we don't use that much xylene or alchohol, i was wondering how other > labs are affected by recycling. > > -- > Yog-Sothoth knows the gate. > Yog-Sothoth is the gate. > Yog-Sothoth is the key and guardian of the gate. Past, present, future, > all are one in Yog-Sothoth. > He knows where the Old Ones broke through of old, and where They shall > break through again. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Pinpoint customers who are looking for what you sell. From mary.abosso <@t> thermofisher.com Wed Oct 3 08:18:24 2007 From: mary.abosso <@t> thermofisher.com (Abosso, Mary) Date: Wed Oct 3 08:18:49 2007 Subject: [Histonet] Re: Ohh the pain (replacing formalin) In-Reply-To: <46FB97DC.CC0E.007B.0@vetmed.ufl.edu> References: <46FB97DC.CC0E.007B.0@vetmed.ufl.edu> Message-ID: <95BEDBE3F30E5446A14D12661707EB0801BDB14C@PGHCR-EXMB-VS-3.na.fshrnet.com> To add on to Shawn's comments, as we were in that nightmare together.... establish a written contract with the medical director and management on how the process will be handled. We thought that we had covered the ground rules, but as Shawn mentioned, the rules went out the window and the histology staff was put through a really stressful, demoralizing experience of jumping through hoops.... and the end result, most of the staff quit, and the doctors took a very long time to be satisfied with the product. This was especially hard to take as it had been a Bench Mark Lab for high quality using an alternative fixative, Prefer. Mary Abosso QA Histotechnologist Thermo Fisher Scientific - Kalamazoo -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shawn Leslie Sent: Thursday, September 27, 2007 11:45 AM To: histonet@lists.utsouthwestern.edu; Douglas D Deltour; Teri' 'Johnson Subject: RE: [Histonet] Re: Ohh the pain (replacing formalin) It's a real hassle changing from either...We had originally used Anatec's Prefer but because HER2Neu is acceptable only on formalin fixed tissue we had to change to formalin...that's when the nightmares began...Everything had to be work up again, H&E..specials, immunos......we had warned the Doc's that there will be reduced quality using formalin instead of Prefer but were told that we had to change and that they will have to accept the quality difference...that was fine and good until the first batch of slides came out and they freaked.....we were told to try this and to try that.....after a while no one knew what was going on because procedural changes were being done on an hourly basis....You could imagine the chaos in the lab.....All I can suggest is that if you have to change either way do it in a organized, systematic and scientific way otherwise everyone will regret it ...I guarantee it..... Shawn Leslie Scientific Research Manager Anatomic Pathology University of Florida School of Veterinary Medicine 352-392-2235 ext 4555 >>> "Douglas D Deltour" 9/27/2007 12:15 PM >>> Teri, don't be surprised. Let's just say that I can not go into detail about the reasoning behind this. I am aware of all of the validation that is involved in this task. At least I hope I can get the "I told you so" in before I am kicked out the door. :) Douglas D. Deltour HT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Teri Sent: Thursday, September 27, 2007 9:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Ohh the pain (replacing formalin) Douglas, if it is your Pathology group pushing you to go to non-formalin fixative, I'm quite surprised. Pathologists are accustomed to making diagnoses based on formalin-based artifacts. In a lab I worked in (in a galaxy far, far away) we did the "Folger's crystals replacement" experiment, replacing all the GI and prostate biopsy formalin fixative with zinc formalin. I thought the pathologists were going to have a major meltdown. The nucleoli were more prominent in all the cells, and that freaked them out. We effectively changed what the cellular structure looked like (even mild cellular changes can be a diagnostically significant). Disregarding the FDA issue and interlaboratory issues already raised (quite valid points!), if you change what fixative you are using, the cells will look different. And while that is not an insurmountable issue for pathologists, it does take getting used to. One possible substitute is glyoxal. Anatech provides this commercially in a fixative called "Prefer". It is supposed to provide formalin-like morphology with heightened immunoreactivity. Remember - ALL your immunohistochemistry staining will have to be redone. ALL OF IT. It is optimised using formalin, and changing the fixative changes the structure of the proteins. Some immunos may work better, some may not work as well, some may no longer require antigen retrieval, some may require a different retrieval method. Do you get any consultation material (outside blocks) from other institutions for second opinion? If so, and you need to do staining of any kind (H&E, special stains, and IHC), you will need to optimise your staining of that material back to formalin. Your non-formalin controls will be useless. I'm not saying this is an impossible task. It will be difficult and seem impossible until it's all worked out. It takes a tremendous amount of effort and energy to make the switch, and above all, the pathologists should have a major stake in how their samples will be fixed, processed, and stained. Their reputation depends on it. Their patient's diagnosis also depends on it. If you want to turn your attention to making formalin safer, there are ways of doing that. That's fodder for another post altogether. Good luck! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ccross6032 <@t> aol.com Wed Oct 3 08:43:00 2007 From: ccross6032 <@t> aol.com (Cheryl Cross) Date: Wed Oct 3 08:43:21 2007 Subject: [Histonet] IHC mapping/morphometrics help? In-Reply-To: <95BEDBE3F30E5446A14D12661707EB0801BDB14C@PGHCR-EXMB-VS-3.na.fshrnet.com> References: <46FB97DC.CC0E.007B.0@vetmed.ufl.edu> <95BEDBE3F30E5446A14D12661707EB0801BDB14C@PGHCR-EXMB-VS-3.na.fshrnet.com> Message-ID: Hi everybody - I am not even sure how to phrase my question appropriately, but i will try... I am working on some IHC of rodent brains. I would like to take IHC stained serial sections and make a 3D map....I know this has been done before with Amira software (which i do not have). I do, however, have OsiriX. I think I can get this to work if I use Adobe Illustrator to map my IHC staining neurons and astrocytes on a regular old mouse brain section template (such as an image from Paxinos, etc). I am, of course, looking for a quicker way to do this :) My problem is that I am working off of photos - I can easily transfer positive "dots" to a blank template at 10, 20, 40x etc...it would be so much faster and easier if there was a quicker way to pick up staining cells at the low power (2x) level. What I find using photoshop is the resolution of the individual positive cells at 2x is poor and they are blurry and throw off my count. This may not make any sense at all the way I have written it - I'm just hoping someone is doing something similar and can give me some tips! Any suggestions appreciated!! Cheryl Cheryl Cross, DVM, Dipl. ACVP Researcher University Corporation for Atmospheric Research College of Veterinary Medicine University of Tennessee Department of Pathology 2407 River Drive, Room A201 Knoxville, TN 37996-4542 (423) 967-2724 fax: 865-974-5616 ccross@ucar.edu From godsgalnow <@t> aol.com Wed Oct 3 08:53:20 2007 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Wed Oct 3 08:53:32 2007 Subject: [Histonet] (no subject) Message-ID: <8C9D3DBE481A8A8-9F0-289B@FWM-D35.sysops.aol.com> ANyone out there using PCNA?? I having been trying to work up this antibody and am not getting anywhere.? I will be using it in conjunction with?to MIB-1.? Since they are both proliferation markers I would expect them to look very similar, but they do not.? The PCNA seems to show different intensities in the staining where MIB-1 is either positive or negative.? I am using breast cancer and colon cancer as my controls.? I have tried using both an HRP and Alk Phos detection.? While the alk phos seems to be a little better than the HRP, it still isn't crisp.? I have the Biocare antibody and titered it out to 1:800 and I am using the Alk Phos Mach-4. Any suggestions? Roxanne ________________________________________________________________________ Email and AIM finally together. You've gotta check out free AOL Mail! - http://mail.aol.com From Charles.Embrey <@t> carle.com Wed Oct 3 09:38:27 2007 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Wed Oct 3 09:38:32 2007 Subject: FW: [Histonet] Recyclers Message-ID: <44780C571F28624DBB446DE55C4D733A1FE550@EXCHANGEBE1.carle.com> -----Original Message----- From: Charles.Embrey Sent: Tuesday, October 02, 2007 3:09 PM To: 'cindy keith'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Recyclers Hi Cindy, Over the years I have worked with the B&R and CBG units but over the last 15 years or so I have stuck with CBG. I put units in at Wright-Patterson AFB before I left the military and then at a lab in Texas I spent a year and a half at. My current lab in Illinois replaced their B&R unit with a CBG recycler before I arrived and I am currently using a 5 gallon solvent unit for my xylene and alcohols and a separate 5 gallon unit for my formalin. Give the company a call and arrange a trial. You have nothing to loose and will be sold on the units from day one. Charles Embrey Jr., PA(ASCP) Carle Clinic, Illinois -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of cindy keith Sent: Monday, October 01, 2007 11:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recyclers Anyone have any recommendations for recyclers? _________________________________________________________________ Invite your mail contacts to join your friends list with Windows Live Spaces. It's easy! http://spaces.live.com/spacesapi.aspx?wx_action=create&wx_url=/friends.a spx&mkt=en-us_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Herrick.James <@t> mayo.edu Wed Oct 3 09:59:35 2007 From: Herrick.James <@t> mayo.edu (Herrick, James L.) Date: Wed Oct 3 09:59:42 2007 Subject: [Histonet] Bone proliferation Message-ID: <572057D3BDD52A46BD05BC6DA50686110CAED2@MSGEBE22.mfad.mfroot.org> Good morning everyone, I have been asked a very general question in regards to a good stain that can be used to show bone proliferation, without any association to BRDU. I don't have an answer but was wondering if any of you might. Thank you very much. Jim From AGrobe2555 <@t> aol.com Wed Oct 3 10:02:27 2007 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Wed Oct 3 10:02:36 2007 Subject: [Histonet] Re:PCNA Message-ID: I'm not having much luck either. I was using colon and esophagus as a positive, and getting variable staining (Histochoice fixed tissue). HIER made the staining worse, not better. I have some skin that I will be trying next. The ab was used at 1:100 to 1:2000, and 1:400 seemed to work best. My impression is that PCNA is quirky at best, and I am thinking about switching to Ki67 instead. Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital ************************************** See what's new at http://www.aol.com From mickie25 <@t> netzero.net Wed Oct 3 10:03:27 2007 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Wed Oct 3 10:03:43 2007 Subject: [Histonet] Recyclers In-Reply-To: <44780C571F28624DBB446DE55C4D733A1FE550@EXCHANGEBE1.carle.com> References: <44780C571F28624DBB446DE55C4D733A1FE550@EXCHANGEBE1.carle.com> Message-ID: Hi Charles and Cindy, I am in agreement with the ease of use of the CBG unit. It is great for Xylene and Xylene Substitutes. I would differ on recycling of alcohol and formalin. When formalin is 'distilled' the buffer salts are removed and must be disposed of. With a Creative Waste Systems formalin recycling unit, the buffer salts are conserved. It is a filtration unit and one need only check ph with minor adjustment and occasionally add some formaldehyde concentrate (using an aldehyde dip stick to keep the formaldehyde at 4%) and no buffer salts to dispose of. Also, their filtration units for recycling alcohol are so easy to use. Just pour the used alcohol in the top and get clean alcohol out the bottom. Call Rex at 503-784-1232 and he will explain in detail. Savings are at least 50% including the cost of the filtration units. Good Luck! Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles.Embrey Sent: Wednesday, October 03, 2007 7:38 AM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] Recyclers -----Original Message----- From: Charles.Embrey Sent: Tuesday, October 02, 2007 3:09 PM To: 'cindy keith'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Recyclers Hi Cindy, Over the years I have worked with the B&R and CBG units but over the last 15 years or so I have stuck with CBG. I put units in at Wright-Patterson AFB before I left the military and then at a lab in Texas I spent a year and a half at. My current lab in Illinois replaced their B&R unit with a CBG recycler before I arrived and I am currently using a 5 gallon solvent unit for my xylene and alcohols and a separate 5 gallon unit for my formalin. Give the company a call and arrange a trial. You have nothing to loose and will be sold on the units from day one. Charles Embrey Jr., PA(ASCP) Carle Clinic, Illinois -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of cindy keith Sent: Monday, October 01, 2007 11:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recyclers Anyone have any recommendations for recyclers? _________________________________________________________________ Invite your mail contacts to join your friends list with Windows Live Spaces. It's easy! http://spaces.live.com/spacesapi.aspx?wx_action=create&wx_url=/friends.a spx&mkt=en-us_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charles.Embrey <@t> carle.com Wed Oct 3 10:19:20 2007 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Wed Oct 3 10:19:25 2007 Subject: [Histonet] Recyclers In-Reply-To: Message-ID: <44780C571F28624DBB446DE55C4D733A1FE551@EXCHANGEBE1.carle.com> I use unbuffered formalin on my processors to keep them cleaner and the rotary valve life expectancy has greatly been increased without ever having to do hot water flushes. When the machines are checked over for Preventive Maintenance the valves and tubing are always in great shape. As to filtration of my alcohols I prefer distillation. My recovered alcohol from the CBG is consistently 97-98% even though I recycle my 70 and 80% from my processors in with the other grades. Charles Embrey, PA(ASCP) -----Original Message----- From: Mickie Johnson [mailto:mickie25@netzero.net] Sent: Wednesday, October 03, 2007 10:03 AM To: Charles.Embrey; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Recyclers Hi Charles and Cindy, I am in agreement with the ease of use of the CBG unit. It is great for Xylene and Xylene Substitutes. I would differ on recycling of alcohol and formalin. When formalin is 'distilled' the buffer salts are removed and must be disposed of. With a Creative Waste Systems formalin recycling unit, the buffer salts are conserved. It is a filtration unit and one need only check ph with minor adjustment and occasionally add some formaldehyde concentrate (using an aldehyde dip stick to keep the formaldehyde at 4%) and no buffer salts to dispose of. Also, their filtration units for recycling alcohol are so easy to use. Just pour the used alcohol in the top and get clean alcohol out the bottom. Call Rex at 503-784-1232 and he will explain in detail. Savings are at least 50% including the cost of the filtration units. Good Luck! Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles.Embrey Sent: Wednesday, October 03, 2007 7:38 AM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] Recyclers -----Original Message----- From: Charles.Embrey Sent: Tuesday, October 02, 2007 3:09 PM To: 'cindy keith'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Recyclers Hi Cindy, Over the years I have worked with the B&R and CBG units but over the last 15 years or so I have stuck with CBG. I put units in at Wright-Patterson AFB before I left the military and then at a lab in Texas I spent a year and a half at. My current lab in Illinois replaced their B&R unit with a CBG recycler before I arrived and I am currently using a 5 gallon solvent unit for my xylene and alcohols and a separate 5 gallon unit for my formalin. Give the company a call and arrange a trial. You have nothing to loose and will be sold on the units from day one. Charles Embrey Jr., PA(ASCP) Carle Clinic, Illinois -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of cindy keith Sent: Monday, October 01, 2007 11:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recyclers Anyone have any recommendations for recyclers? _________________________________________________________________ Invite your mail contacts to join your friends list with Windows Live Spaces. It's easy! http://spaces.live.com/spacesapi.aspx?wx_action=create&wx_url=/friends.a spx&mkt=en-us_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kimtournear <@t> yahoo.com Wed Oct 3 10:43:34 2007 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Wed Oct 3 10:43:39 2007 Subject: [Histonet] re: PCNA Message-ID: <276742.64754.qm@web50612.mail.re2.yahoo.com> We always used the Ki67 over the PCNA....docs liked it better and it was always consistent. Fixative subs can also be issues with HIER and/or enzyme digestion...typically you wouldn't need a harsh HIER or digestion, if any at all, to achieve optimal staining....but not always the case...you just have to be careful of the false (+) and false (-) results.... If you think about it, most detection kits and antibodies are worked up with 10% NBF for most companies....it's a whole different ball game with fixative subs and, in my world requires too much and too little time to work up new abs...so we stayed with formalin.... Just my 2 cents worth.... Kim Tournear, HT (ASCP), QIHC ( ASCP) Specialists in Dermatology Histology/Mohs Supervisor Tucson, AZ --------------------------------- Be a better Heartthrob. Get better relationship answers from someone who knows. Yahoo! Answers - Check it out. From Linke_Noelle <@t> allergan.com Wed Oct 3 10:46:34 2007 From: Linke_Noelle <@t> allergan.com (Linke_Noelle) Date: Wed Oct 3 10:48:30 2007 Subject: [Histonet] job opening In-Reply-To: <8C9D3DBE481A8A8-9F0-289B@FWM-D35.sysops.aol.com> Message-ID: <5C3DA4BE34AA0641BAA10A7C1478B60502EBB0@IRMAIL132.irvine.allergan.com> Histotech Allergan, Inc. The Company: With more than 55 years of experience providing high-quality, science-based products, Allergan, Inc., with headquarters in Irvine, California, discovers, develops and commercializes products in the ophthalmology, neurosciences, medical dermatology, medical aesthetics, obesity intervention and other specialty markets that deliver value to its customers, satisfy unmet medical needs, and improve patients' lives. The Opportunity: Primary responsibilities include collection and trimming, specimen processing, embedding, sectioning, staining, and coverslipping, in a GLP regulated research facility. The Requirements: Position requires minimum 2 years of relevant experience and HT/HTL ASCP certification, BS preferred. Competency with PC based standard office software, such as MS Word, EXCEL and PowerPoint desirable. The successful candidate will be a team player, well organized, methodical, detail oriented, self motivated, focused, dependable, and take pride in their work Please apply online using the following link: http://www.allergan.com/site/careers/home.asp and type in position number 50060562 in the search box at the bottom of the page. Feel free to contact me as well if you have any questions. No?lle Linke, MS, HTL(ASCP)QIHC Allergan, Inc 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5568 From slappycraw <@t> yahoo.com Wed Oct 3 10:49:16 2007 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Wed Oct 3 10:49:21 2007 Subject: [Histonet] Langarin antibody Message-ID: <924144.28561.qm@web53607.mail.re2.yahoo.com> Anybody out there doing Langarin on mouse FFPE tissue? If so, did you use AR, and did it work? Thanks Larry A. Woody Amgen Seattle, Wa. --------------------------------- Be a better Heartthrob. Get better relationship answers from someone who knows. Yahoo! Answers - Check it out. From cfranci <@t> rigel.com Wed Oct 3 11:02:17 2007 From: cfranci <@t> rigel.com (Christian Franci) Date: Wed Oct 3 11:02:59 2007 Subject: [Histonet] the ongoing saga of muCD31 staining Message-ID: <107a1607d3db33dfcc6bd23ea040783c@rigel.com> hey you zany histokids! All told, Iv'e gotten several Ab's to work on FFPE sections. The staining is reproducible, clean but it's never super strong. It seems that any time you have to do enzyme digestion there's a VERY FINE line between under-digestion and over-digestion of the section and when you have several dozen to do at once, well, the timing is not always precise.... anyhow, I found a nice paper in Nature from 2006 from Gavin Thurston's group over at Regeneron. They have these beautiful images of mouse blood vessels. Of course, being a Nature paper, the details are rather vague... but, I noticed something interesting... they used pyronin-y as a counter-stain not hematox. I've personally never used this or heard of it... Have any of you used this stain? is it simple to use or are there "tricks" involved? Can it be used in FFPE sections or just in frozen ones? As usual, thanks in advance for any help you can give me. Cheers Chris From b-frederick <@t> northwestern.edu Wed Oct 3 11:28:41 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Oct 3 11:28:47 2007 Subject: [Histonet] Re:PCNA In-Reply-To: Message-ID: <000001c805da$78f6aaa0$d00f7ca5@lurie.northwestern.edu> We were (it's been a while) running the Dako PCNA at 1:10000 with a tonsil control ph6 30/15 using the envision+ detection from Dako. Ki-67 is requested more for a lot of the studies we deal with. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of AGrobe2555@aol.com Sent: Wednesday, October 03, 2007 10:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re:PCNA I'm not having much luck either. I was using colon and esophagus as a positive, and getting variable staining (Histochoice fixed tissue). HIER made the staining worse, not better. I have some skin that I will be trying next. The ab was used at 1:100 to 1:2000, and 1:400 seemed to work best. My impression is that PCNA is quirky at best, and I am thinking about switching to Ki67 instead. Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital ************************************** See what's new at http://www.aol.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carmen_loiselle <@t> hotmail.com Wed Oct 3 11:41:03 2007 From: carmen_loiselle <@t> hotmail.com (carmen loiselle) Date: Wed Oct 3 11:41:07 2007 Subject: [Histonet] TFE-3, INI AND MYOD ANTIBODIES Message-ID: I would greatly appreciate any information regarding these 3 antibodies. It has been requested by our pathologists. It's for IHC ,using paraffin sections on Ventana immunostainers (BMK & XT). What would be the best source and protocols ? I'll appreciate any input for these markers Thanks to all _________________________________________________________________ Envoie un sourire, fais rire, amuse-toi! Employez-le maintenant! http://www.emoticonesgratuites.ca/?icid=EMFRCA120 From jcline <@t> wchsys.org Wed Oct 3 12:14:59 2007 From: jcline <@t> wchsys.org (Joyce Cline) Date: Wed Oct 3 12:15:13 2007 Subject: [Histonet] Re/ Recyclers Message-ID: <000801c805e0$ee3e4290$1d2a14ac@wchsys.org> We use the CBG 5 gallon recycler for alcohol and substitute. The alcohol comes out at 96 to 97 % every time. (recycling 95 to 100 alcohols). We use CBG's Formula 83 which is a substitute for Xylene. Formula 83 is as close to Xylene that we have found, dries quickly for slides and processes fine. Formula 83 recycles very well. We do not use anything but vendor pure reagents for deparaffinization and dehydration of our specials and IHC by machine. ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From mtarango <@t> nvcancer.org Wed Oct 3 13:40:00 2007 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Wed Oct 3 13:40:19 2007 Subject: [Histonet] the ongoing saga of muCD31 staining In-Reply-To: <107a1607d3db33dfcc6bd23ea040783c@rigel.com> Message-ID: <5AEC610C1CE02945BD63A395BA763EDE011B6E95@NVCIEXCH02.NVCI.org> You can use it on paraffin-embedded tissue. Pyronin-Y stains RNA pink. Lots of people use this with the methyl-green pyronin-Y stain. It's to show DNA and RNA in two different colors. Methyl green stains DNA greenish-blue and pyronin-Y stains RNA pink. We sometimes use this when looking for plasma cells, if the doc can't wait for a CD138 immunostain. I've never used it as a counterstain for IHC. Mark Adam Tarango HT(ASCP) Histology & IHC Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 Direct Line (702) 822-5112 Mobile (702) 759-9229 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Christian Franci Sent: Wednesday, October 03, 2007 9:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] the ongoing saga of muCD31 staining hey you zany histokids! All told, Iv'e gotten several Ab's to work on FFPE sections. The staining is reproducible, clean but it's never super strong. It seems that any time you have to do enzyme digestion there's a VERY FINE line between under-digestion and over-digestion of the section and when you have several dozen to do at once, well, the timing is not always precise.... anyhow, I found a nice paper in Nature from 2006 from Gavin Thurston's group over at Regeneron. They have these beautiful images of mouse blood vessels. Of course, being a Nature paper, the details are rather vague... but, I noticed something interesting... they used pyronin-y as a counter-stain not hematox. I've personally never used this or heard of it... Have any of you used this stain? is it simple to use or are there "tricks" involved? Can it be used in FFPE sections or just in frozen ones? As usual, thanks in advance for any help you can give me. Cheers Chris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From PMEarle <@t> CapeCodHealth.org Wed Oct 3 14:02:12 2007 From: PMEarle <@t> CapeCodHealth.org (Earle, Paul M.) Date: Wed Oct 3 14:02:18 2007 Subject: [Histonet] Xylene Recycler Message-ID: <29688FCA0750AD4FB55B18D961EB91CC10172A@cchex2.cchdomain1.capecodhealth.org> Cindy, We also have been using the CBG 5 gallon recyler for our xylene . Like others have mentioned, it is easy to use and will certainly pay for itself quickly. Paul Earle Paul M. Earle M.S., P.A. (ASCP) Anatomic Pathology Supervisor Pathology Department Falmouth Hospital 100 Ter Heun Drive Falmouth, MA 02540 508-548-5300 x73488 <> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org From treese <@t> sleh.com Wed Oct 3 16:28:35 2007 From: treese <@t> sleh.com (Reese, Tommy G.) Date: Wed Oct 3 16:28:48 2007 Subject: [Histonet] new products Message-ID: <023DBFA505E67A4689A2CEEC40AE3F7D8B977076DC@SLEHEXCH02.sleh.com> I was wondering if anyone had been working with or had any knowledge of a couple of products from Diagnostic Biosystems. They are the Histozyme retrieval system and the Intensi/fire DAB enhancement system. I've been using the Histozyme in place of both pepsin and trypsin for 5 minutes at room temperature. The preliminary results have been outstanding. I'ts a big time saver. The enhancement solution has allowed me to use my antibodies at one tenth of the dilutions I normally use. This week I'm in the process of diluting them even further to see what the results are. I'm hoping it will allow me to stop using edta as much as possible and only use citrate retrieval. If anyone has any experience with either of these products please advise me as to what you are doing and how they are working. +++++CONFIDENTIALITY NOTICE+++++ The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. From Jason.Burrill <@t> crl.com Wed Oct 3 22:26:23 2007 From: Jason.Burrill <@t> crl.com (Burrill, Jason) Date: Wed Oct 3 22:26:44 2007 Subject: [Histonet] GDP or GLP Documentation Training Message-ID: <1AD4E907E9B6F648AEF1B3A20A9B0E1E0BDEC5@shr-exch2.na01.crl.com> We have had employees go to this training and they have said it was very useful and it is in PA. http://www.cfpie.com/showitem.aspx?productid=040 Jason Burrill Manager, Histology Charles River Laboratories 251 Ballardvale St Wilmington, MA 01887 Phone: 978-658-6000 ext. 1652 Fax: 978-988-8793 jason.burrill@crl.com From koellingr <@t> comcast.net Wed Oct 3 23:39:29 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Wed Oct 3 23:39:35 2007 Subject: [Histonet] Re:PCNA/MIB/proliferation Message-ID: <100420070439.20059.47046E810006D3E400004E5B22007348409D09020704040A0105@comcast.net> Raoxanne, There have been several posts just now concerning proliferation and the "quirkiness" or "messiness" or "different intensities" with PCNA. Here is my experience. PCNA is an antigen that is a nuclear, auxiliary molecule during proliferation. So in theory it is around during G1-S-G2-M when cell is proliferating. But cells spend most time in G0 (quiescent phase). Yet PCNA has a very long half-life and it certainly is present into G0 phase long after the proliferative, mitotic event. That is in literature and I have certainly seen that in cell lines and cells made to be "in synch" for their cell cycle. So you see some spectrum of PCNA intensity in what are actually non-proliferating cells, it can be quite true. Could be just left over. On the other hand the MIB nuclear protein, that a lot of people detect with the Ki-67 epitope antibody is relatively VERY short lived. It is there, and necessary, during proliferation, and as soon as that event is winding down in M phase, MIB is long gone. So it is "on and off" as opposed to on and then still there and then a little still there. Knowing the biology, and throwing into the mix fixation va riance and section thickness variance and DNA repair possibilities and other things, I think it just turns out that MIB is easier to interpret and follow although PCNA, if you can read through the dirtiness, is still a good marker. For me, staining for MIB tracked very directly to gold standard BrdU staining and PCNA was also there but tough to interpret compared to BrdU. And I far prefer HRP to any alk phos in discrimination of interpretation of any of these but that is just a personal preference. Ray Koelling PhenoPath Laboratories Seattle, WA -------------- Original message -------------- From: godsgalnow@aol.com > ANyone out there using PCNA?? I having been trying to work up this antibody and > am not getting anywhere.? I will be using it in conjunction with?to MIB-1.? > Since they are both proliferation markers I would expect them to look very > similar, but they do not.? The PCNA seems to show different intensities in the > staining where MIB-1 is either positive or negative.? > > I am using breast cancer and colon cancer as my controls.? I have tried using > both an HRP and Alk Phos detection.? While the alk phos seems to be a little > better than the HRP, it still isn't crisp.? I have the Biocare antibody and > titered it out to 1:800 and I am using the Alk Phos Mach-4. > > Any suggestions? > > Roxanne > ________________________________________________________________________ > Email and AIM finally together. You've gotta check out free AOL Mail! - > http://mail.aol.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BMolinari <@t> heart.thi.tmc.edu Thu Oct 4 05:48:10 2007 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Thu Oct 4 05:48:14 2007 Subject: [Histonet] Sucrose and paraffin Message-ID: Hi all, I have no experience with IHC but (once again) had a researcher ask me if placing tissue in sucrose after 10% NBF is helpful in antigen staining. Thanks, Betsy Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) From trumpy50 <@t> hotmail.com Thu Oct 4 08:00:43 2007 From: trumpy50 <@t> hotmail.com (Jennifer Trump) Date: Thu Oct 4 08:00:50 2007 Subject: [Histonet] microwave processing Message-ID: Hello all, I am starting up a Histology Lab in a urology practice. I will be processing prostates using the TBS shurwave 1200. I have read all about using Preserve as a fixative but would like to have some advice from you all before I go ahead and implement that. I know a minimum of 12 hours of fixing at RT in formalin is preferable but what is the minimum amount of time I can get away with? Of course they want same day turn! Thanks! Jenny _________________________________________________________________ Peek-a-boo FREE Tricks & Treats for You! http://www.reallivemoms.com?ocid=TXT_TAGHM&loc=us From BMolinari <@t> heart.thi.tmc.edu Thu Oct 4 08:24:35 2007 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Thu Oct 4 08:24:38 2007 Subject: [Histonet] Sucrose and paraffin In-Reply-To: <47049C90.CC0E.007B.0@vetmed.ufl.edu> Message-ID: Shawn, I have heard of sucrose for cryoprotection, but these are tissues that have been fixed in 10% NBF and they want to then put in sucrose then process for paraffin. I just don't understand the rational or if there would be any advantage for IHC. Betsy -----Original Message----- From: Shawn Leslie [mailto:LeslieS@vetmed.ufl.edu] Sent: Thursday, October 04, 2007 6:57 AM To: Molinari, Betsy Subject: Re: [Histonet] Sucrose and paraffin I used sucrose in research for cryo protection. We used 10, 20. and 30% sucrose to protect the tissue from freeze artifact as we were doing frozen sections on it. I don't think that sucrose will do anything for antigen staining.... Shawn Leslie Scientific Research Manager Anatomic Pathology University of Florida School of Veterinary Medicine 352-392-2235 ext 4555 >>> "Molinari, Betsy" 10/4/2007 6:48 AM >>> Hi all, I have no experience with IHC but (once again) had a researcher ask me if placing tissue in sucrose after 10% NBF is helpful in antigen staining. Thanks, Betsy Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BMolinari <@t> heart.thi.tmc.edu Thu Oct 4 08:26:13 2007 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Thu Oct 4 08:26:16 2007 Subject: [Histonet] Sucrose and paraffin In-Reply-To: Message-ID: Thank you. I though as much but since my background is not in IHC I wanted to be sure. Betsy -----Original Message----- From: Edwards, R.E. [mailto:ree3@leicester.ac.uk] Sent: Thursday, October 04, 2007 8:10 AM To: Molinari, Betsy Subject: RE: [Histonet] Sucrose and paraffin Would make no difference... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: 04 October 2007 11:48 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Sucrose and paraffin Hi all, I have no experience with IHC but (once again) had a researcher ask me if placing tissue in sucrose after 10% NBF is helpful in antigen staining. Thanks, Betsy Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From garth <@t> apollosci.co.za Thu Oct 4 08:36:33 2007 From: garth <@t> apollosci.co.za (Garth Jerome) Date: Thu Oct 4 08:37:26 2007 Subject: [Histonet] microwave processing In-Reply-To: Message-ID: <003701c8068b$960db280$7700a8c0@jhb.apollosci.co.za> Hello Jennifer Whilst I do not have SPECIFIC data for the Shurwave, we process prostate biopsies in our Milestone unit, using FineFix. For biopsies, where fixation is uncertain, we fix for 20mins in the microwave in FineFix or 10%NBF. Both work equally well. This is a 800W magnetron and Temp is 50 degree Celsius. Hope this can help you. Regards Garth Jerome Apollo Scientific cc Telephone : 27-11-466 7666 Facsimile : 27-11-466 7672 Personal facsimile : 086 660 0539 Cell phone : 084 504 1101 Email : garth@apollosci.co.za -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Trump Sent: 04 October 2007 03:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] microwave processing Hello all, I am starting up a Histology Lab in a urology practice. I will be processing prostates using the TBS shurwave 1200. I have read all about using Preserve as a fixative but would like to have some advice from you all before I go ahead and implement that. I know a minimum of 12 hours of fixing at RT in formalin is preferable but what is the minimum amount of time I can get away with? Of course they want same day turn! Thanks! Jenny _________________________________________________________________ Peek-a-boo FREE Tricks & Treats for You! http://www.reallivemoms.com?ocid=TXT_TAGHM&loc=us___________________________ ____________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Thu Oct 4 08:54:22 2007 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Thu Oct 4 08:52:02 2007 Subject: [Histonet] Americlear problem? Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F4D0@lmhsmail.lmhealth.org> For those of you who use Americlear.... Last Friday we changed out our tissue processor. On Monday, the tissue did not cut well at all. The tissue had not cleared at all. I check the reagent containers and to the best that I could determine, all appropriate reagents were in the proper containers. The only thing I noticed was that the Americlear just didn't seem right. Seemed to be a littler watery. All of the same lot # was used on the processor on Friday. I changed it out on Monday with a different lot # and all is fine again. Just wondering if anyone else has experienced this? I can't find an actual website for Stephen's Scientific to check for notices. Thanks Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org From Kemlo.Rogerson <@t> waht.swest.nhs.uk Thu Oct 4 08:56:43 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Oct 4 08:56:53 2007 Subject: [Histonet] Americlear problem? Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222EE57@wahtntex2.waht.swest.nhs.uk> What's Americlear? Do we have Briticlear? What's in it? Spect you don't know cos it's a secret. That's what comes of using proprietary stuff you don't know what they put in it, and what they put in it by mistake. Nothing like using a good health carcinogen like xylene. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Giving is more a dictate of the heart than a command of the brain. --Henry A. Russo This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From mpence <@t> grhs.net Thu Oct 4 09:24:01 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Oct 4 09:26:27 2007 Subject: [Histonet] Americlear problem? In-Reply-To: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F4D0@lmhsmail.lmhealth.org> Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C744@IS-E2K3.grhs.net> A long, long time ago in a far away place...... Just kidding! I used Americlear many years ago and we had similar problems from time to time. Many times the wrong solution was placed in the container. The problem with Americlear is that the entire lab and the containers and the blocks and your body starts to smell like oranges. I have went back to blocks processed 20 years ago and they still smell like oranges. My techs began to taste metal in their months and that is when we went to Propar. My thought of the day! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Thursday, October 04, 2007 8:54 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Americlear problem? For those of you who use Americlear.... Last Friday we changed out our tissue processor. On Monday, the tissue did not cut well at all. The tissue had not cleared at all. I check the reagent containers and to the best that I could determine, all appropriate reagents were in the proper containers. The only thing I noticed was that the Americlear just didn't seem right. Seemed to be a littler watery. All of the same lot # was used on the processor on Friday. I changed it out on Monday with a different lot # and all is fine again. Just wondering if anyone else has experienced this? I can't find an actual website for Stephen's Scientific to check for notices. Thanks Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From FUNKM <@t> mercyhealth.com Thu Oct 4 09:53:19 2007 From: FUNKM <@t> mercyhealth.com (Marcia Funk) Date: Thu Oct 4 09:53:50 2007 Subject: [Histonet] Space for Sectioning Message-ID: <4704B80F.9B87.00AC.0@mercyhealth.com> I'm looking for data if anyone has some to share. We are remolding our Histo Lab, working with management they would like to see data on how much space is needed for each sectioning area. If you have any information I would appreciate you time. Thanks in advance. Marcia from the Northland From jmahoney <@t> alegent.org Thu Oct 4 10:09:06 2007 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Thu Oct 4 10:09:24 2007 Subject: [Histonet] Space for Sectioning In-Reply-To: <4704B80F.9B87.00AC.0@mercyhealth.com> References: <4704B80F.9B87.00AC.0@mercyhealth.com> Message-ID: <4704BBC20200003C0001CB18@gwia.alegent.org> Hello, I have studied this pretty extensively. I think that the minimum counter width is 3.5 feet but optimally 4 feet. The cutting surface should be deep enough (about 30") so that supplies can be stored in bins toward the back of the counter while space for labeling trimming, rack, etc. can be toward the front. Good luck with your project Marcia. Jan Mahoney Alegent Health, Omaha NE >>> "Marcia Funk" 10/04/2007 9:53 AM >>> I'm looking for data if anyone has some to share. We are remolding our Histo Lab, working with management they would like to see data on how much space is needed for each sectioning area. If you have any information I would appreciate you time. Thanks in advance. Marcia from the Northland _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Thu Oct 4 10:18:31 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu Oct 4 10:19:14 2007 Subject: [Histonet] Bone Saws In-Reply-To: <4704B80F.9B87.00AC.0@mercyhealth.com> Message-ID: Does anyone have a suggestion for a good electric bone saw for histology? My intent is to trim un-decalcified bone to 3mm to facilitate/speed up decalcification. Are there any rules governing bone dust? I remember years ago, a pathologist complaining that anything but a hacksaw creating a burning artifact in the bone. Any thoughts on this? Jackie From cforster <@t> umn.edu Thu Oct 4 10:48:08 2007 From: cforster <@t> umn.edu (Colleen Forster) Date: Thu Oct 4 10:48:16 2007 Subject: [Histonet] leukocyte IHC Message-ID: <47050B38.30605@umn.edu> Hello Histonetters, I have been asked the following question. What are others using for IHC on leukocytes?? We are considering adding a leukocyte stain for some of the organ slides. What would you recommend? We have measured MPO activity in the organ homogenates and see significant changes with CO treatment. Would you recommend MPO, or some other confirmatory stain? Thanks in advance for your help. Colleen Forster U of MN From ree3 <@t> leicester.ac.uk Thu Oct 4 10:59:47 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Thu Oct 4 10:59:59 2007 Subject: [Histonet] Bone Saws In-Reply-To: References: <4704B80F.9B87.00AC.0@mercyhealth.com> Message-ID: Suggest you watch the movie "SAW 4", whatever they used seemed pretty effective. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: 04 October 2007 16:19 To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] Bone Saws Does anyone have a suggestion for a good electric bone saw for histology? My intent is to trim un-decalcified bone to 3mm to facilitate/speed up decalcification. Are there any rules governing bone dust? I remember years ago, a pathologist complaining that anything but a hacksaw creating a burning artifact in the bone. Any thoughts on this? Jackie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tjasper <@t> copc.net Thu Oct 4 11:08:34 2007 From: tjasper <@t> copc.net (Thomas Jasper) Date: Thu Oct 4 11:08:47 2007 Subject: [Histonet] Space for Sectioning References: <4704B80F.9B87.00AC.0@mercyhealth.com> Message-ID: <90354A475B420441B2A0396E5008D4965E1FC2@copc-sbs.COPC.local> Hi Marcia, Don't know exactly where you're at in the Northland (I suspect Minnesota) and I don't have any hard data for you (so why am I writing this)? Well, about 10 years ago, in Duluth, we carried out a little project before a major renovation. We sent people out to other hospitals with a video camera. Permission granted ahead of time, of course. We got some film footage from Minneapolis, Milwaukee and Green Bay. Once we had all the tape we sat down and looked it over closely and identified what we liked and didn't like. This helped us a great deal in deciding what kind of changes would work best for us. Renovations in particular are tough as most often you don't have a clean slate and there are bound to be limits on what can and cannot be done. Like I said, no hard data, but maybe an idea or too worth implementing or avoiding. If you are in Minnesota I'd be willing to bet that the fine folks where I used to work in Duluth would be helpful to you. Let me know and I can put you in touch with them. Good Luck, Tom Jasper Thomas Jasper HT(ASCP)BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 tjasper@copc.net 541/693-2677 ext. 4050 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcia Funk Sent: Thursday, October 04, 2007 7:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Space for Sectioning I'm looking for data if anyone has some to share. We are remolding our Histo Lab, working with management they would like to see data on how much space is needed for each sectioning area. If you have any information I would appreciate you time. Thanks in advance. Marcia from the Northland _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ian.montgomery <@t> bio.gla.ac.uk Thu Oct 4 11:10:26 2007 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Thu Oct 4 11:10:39 2007 Subject: [Histonet] Histochoice clearing agent. Message-ID: <003101c806a1$1390b0d0$6424d182@IBLS.GLA.AC.UK> Before I buy a container as a trial, any comments? Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, G12 8QQ. From ree3 <@t> leicester.ac.uk Thu Oct 4 11:13:11 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Thu Oct 4 11:13:23 2007 Subject: [Histonet] Space for Sectioning In-Reply-To: <90354A475B420441B2A0396E5008D4965E1FC2@copc-sbs.COPC.local> References: <90354A475B420441B2A0396E5008D4965E1FC2@copc-sbs.COPC.local> Message-ID: Just make sure you include enough power sockets in your plans , estimate how many you need and then add half again. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: 04 October 2007 17:09 To: Marcia Funk Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Space for Sectioning Hi Marcia, Don't know exactly where you're at in the Northland (I suspect Minnesota) and I don't have any hard data for you (so why am I writing this)? Well, about 10 years ago, in Duluth, we carried out a little project before a major renovation. We sent people out to other hospitals with a video camera. Permission granted ahead of time, of course. We got some film footage from Minneapolis, Milwaukee and Green Bay. Once we had all the tape we sat down and looked it over closely and identified what we liked and didn't like. This helped us a great deal in deciding what kind of changes would work best for us. Renovations in particular are tough as most often you don't have a clean slate and there are bound to be limits on what can and cannot be done. Like I said, no hard data, but maybe an idea or too worth implementing or avoiding. If you are in Minnesota I'd be willing to bet that the fine folks where I used to work in Duluth would be helpful to you. Let me know and I can put you in touch with them. Good Luck, Tom Jasper Thomas Jasper HT(ASCP)BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 tjasper@copc.net 541/693-2677 ext. 4050 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcia Funk Sent: Thursday, October 04, 2007 7:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Space for Sectioning I'm looking for data if anyone has some to share. We are remolding our Histo Lab, working with management they would like to see data on how much space is needed for each sectioning area. If you have any information I would appreciate you time. Thanks in advance. Marcia from the Northland _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From schaundrawalton <@t> yahoo.com Thu Oct 4 11:21:25 2007 From: schaundrawalton <@t> yahoo.com (Schaundra Walton) Date: Thu Oct 4 11:21:33 2007 Subject: [Histonet] Formalin Neutralization Message-ID: <564096.80444.qm@web58912.mail.re1.yahoo.com> Has anyone used Market Lab's Hyde-Away to neutralize 10% NBF? Schaundra Walton BS HTL(ASCP) Swedish American Hospital 1401 E. State St. Rockford, IL 61104 --------------------------------- Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. From tissuearray <@t> hotmail.com Thu Oct 4 11:25:20 2007 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Thu Oct 4 11:25:25 2007 Subject: [Histonet] Tissue Microarray Technician Employment? Message-ID: Does anyone know what the going rate for an experienced TMA Tech is paid these days. I realize the field is very new and nothing is set yet to my knowledge. I am an (ASCP) HT and have been creating TMAs since 2000. I have several articles published in the Journal of Histotechnology on constructing TMAs and did a workshop at the NSH in North Carolina on TMA construction. Also I am the inventor of the block warmer, making it easier to punch TMAs. And the Scraping Technique for creating arrays using very small samples of tissue. Just wondering if there is employment out there for someone with my technicial skills? My website is: www.arrayworkshop.com I consider this a resume of my TMA work and other contrubutions to my field. Thank you, Thom Jensen HT(ASCP)/TMA Technician _________________________________________________________________ Peek-a-boo FREE Tricks & Treats for You! http://www.reallivemoms.com?ocid=TXT_TAGHM&loc=us From mhanna <@t> histosearch.com Thu Oct 4 11:31:07 2007 From: mhanna <@t> histosearch.com (Marvin Hanna) Date: Thu Oct 4 11:31:20 2007 Subject: [Histonet] Histology Job Openings Message-ID: <697EC144-2325-48E2-BE7A-B0BC54C3E121@histosearch.com> Hi All, These are the latest job openings listed on Histosearch: Histotechnician - Greensboro, NC - Greensboro Pathology Associates Histo Tech - Orlando and St. Petersburg, FL - Relia Solutions for Histology Professionals Histotechnologist - Altamonte Springs, FL - Aerotek Scientific - 70000.00 Per Year Histotech - Tampa, FL - Gulf Coast Dermatopathology IHC Technician - Waco, TX - Central Texas Pathology Laboratory Histologist - St. Louis, MO - Histotech Exchange LLC MOHS Histotech/part time - Lee's Summit, MO (Kansas City) - Lee's Summit Dermatology Associates, PC Histology Technician - Columbia,SC - Professional Pathology Services, PC Histologist - Bend, OR - Central Oregon Regional Pathology Services Product Support Specialist - Newcastle, UK - Genetix Histotechnologist - Ephrata, PA - Ephrata Community Hospital Histotech - Sonoma County, CA - Sonoma County Dermatology Lab Histology Associate - North Chicago, IL - Abbott Laboratories Histotech - Solotna, AK - Peninsula Pathology Institute Histology Technician-Research Associate - San Diego, CA - Pfizer, Inc Histotechnologist (HTL) - Minneapolis, MN - University of Minnesota Medical Center, Fairview Job postings are free. Best Regards, Marvin Hanna www.histosearch.com jobs.histosearch.com www.histoauctions.com From rjbuesa <@t> yahoo.com Thu Oct 4 11:34:38 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 4 11:34:43 2007 Subject: [Histonet] Tissue Microarray Technician Employment? In-Reply-To: Message-ID: <804235.22462.qm@web61216.mail.yahoo.com> Never, ever try to negotiate a salary as "part of a group". Eveluate yourself and your needs, consider your experience and ask what you think is fair and, of course, never accept the first offer. On tsalary negotiations both potential employer and employee are just trying to get advantage of each other. Salary rates in histology are so wacky that you may have different pay rates within the same lab, or "accross the street". Ren? J. Thom Jensen wrote: Does anyone know what the going rate for an experienced TMA Tech is paid these days. I realize the field is very new and nothing is set yet to my knowledge. I am an (ASCP) HT and have been creating TMAs since 2000. I have several articles published in the Journal of Histotechnology on constructing TMAs and did a workshop at the NSH in North Carolina on TMA construction. Also I am the inventor of the block warmer, making it easier to punch TMAs. And the Scraping Technique for creating arrays using very small samples of tissue. Just wondering if there is employment out there for someone with my technicial skills? My website is: www.arrayworkshop.com I consider this a resume of my TMA work and other contrubutions to my field. Thank you, Thom Jensen HT(ASCP)/TMA Technician _________________________________________________________________ Peek-a-boo FREE Tricks & Treats for You! http://www.reallivemoms.com?ocid=TXT_TAGHM&loc=us_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better Globetrotter. Get better travel answers from someone who knows. Yahoo! Answers - Check it out. From CIngles <@t> uwhealth.org Thu Oct 4 11:37:47 2007 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Thu Oct 4 11:37:57 2007 Subject: [Histonet] OT: Halloween for Histotechs References: <564096.80444.qm@web58912.mail.re1.yahoo.com> Message-ID: <08A0A863637F1349BBFD83A96B27A50A120081@uwhis-xchng3.uwhis.hosp.wisc.edu> Hey 'Netters! Ya OK, delete this if not interested. I was in Walgreens this morning and came across some interesting things for decorating. My favorite is a hanging sign that says "Butcher Shop" and has a picture of a hatchet and some disembodied fingers. Needless to say I laughed out loud and got it to put up in the lab. Lots of other people thought we should put it in the waiting area, (We are a Mohs lab with a large volume. i.e. about 100 Mohs flats and 50 biopsies yesterday) But I get into enough trouble so I erred on the side of a little sanity (Emphasis on 'little'). But I digress, even further. The signs run for about $8. They also have various plastic bugs in test tubes for a $1. Not to mention tons of other little things that are pretty cheap but still look cool. Your colleague in gallows humor, Claire Ingles UW Hospital Madison WI Hey come on, we have to be a little crazy to keep from going totally insane. From Charlotte.Kopczynski <@t> baycare.org Thu Oct 4 12:10:51 2007 From: Charlotte.Kopczynski <@t> baycare.org (Kopczynski, Charlotte) Date: Thu Oct 4 12:11:06 2007 Subject: [Histonet] Part Time Histotech Needed In-Reply-To: <696khu$1gut2d@intercon.baycare.org> Message-ID: We desperately need a histotech to work Part time for Morton Plant Hospital, Mease Dunedin Hospital and Mease Countryside Hospital in Clearwater/Dunedin, Florida. Please contact me or check baycarejobs.com Thanks, Charlotte Kopczynski,HTL(ASCP) Regional Pathology Manager BayCare Laboratories Phone: 727-461-8246 Fax: 727-462-7596 Confidential: This electronic message and all contents contain information from BayCare Health System which may be privileged, confidential or otherwise protected from disclosure. The information is intended to be for the addressee only. If you are not the addressee, any disclosure, copy, distribution or use of the contents of this message is prohibited. If you have received this electronic message in error, please notify the sender and destroy the original message and all copies. From Shirley_PHUA <@t> hsa.gov.sg Thu Oct 4 13:03:52 2007 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Thu Oct 4 13:09:44 2007 Subject: [Histonet] Shirley Phua is away on holiday 05-08 October 2007. Message-ID: I will be out of the office from 05-10-2007 to 08-10-2007. I'll be away on holiday 05-08 October 2007. I'll be back on 09 October 2007 (Tuesday). Pathologists: I will process your requests when I return. If urgent, please forward your email to Henry_Kyaw@hsa.gov.sg From ROrr <@t> enh.org Thu Oct 4 13:10:45 2007 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Thu Oct 4 13:11:01 2007 Subject: [Histonet] Bone saw Message-ID: Hi Jackie, Check out http://www.mar-med.com/bonesaw.html We use their cryovac hoses. They are based near Cleveland OH. Hope this helps. Is it Friday yet? Becky Orr CLA,HT(ASCP)QIHC Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 From lisab <@t> hollandhospital.org Thu Oct 4 13:14:42 2007 From: lisab <@t> hollandhospital.org (Lisa Brenner) Date: Thu Oct 4 13:15:05 2007 Subject: [Histonet] antibodies Message-ID: <4704F551.3CA1.003C.0@hollandhospital.org> Hello, We have a pathologist who would like a triple stain. p63, high molecular weight CK, and racemase. Has anyone used this? Where do you find it? Please any information you could give me would be greatly appreciated. Lisa Brenner HTL (ASCP) Histology Technical Consultant Holland Hospital phone: (616)394-3184 lisab@hollandhospital.org Confidentiality Notice: The information contained in this e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information, or Protected Health Information as such term is defined under the Health Insurance Portability and Accountability Act of 1996 (HIPAA). Any unauthorized review, use, disclosure, copying or distribution is prohibited and may be unlawful. If you believe you have received this e-mail in error, please contact the sender by reply e-mail and delete all copies of the original message, including attachments. From rcharles <@t> state.pa.us Thu Oct 4 13:17:12 2007 From: rcharles <@t> state.pa.us (Charles, Roger) Date: Thu Oct 4 13:18:41 2007 Subject: [Histonet] Shirley Phua is away on holiday 05-08 October 2007. Message-ID: <12E4E17FEF6EBE4BAE95BEB3CDCB57000C79CF62@enhbgpri04.backup> I Shirley ever not away? Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley PHUA Sent: Thursday, October 04, 2007 2:04 PM To: histonet Subject: [Histonet] Shirley Phua is away on holiday 05-08 October 2007. I will be out of the office from 05-10-2007 to 08-10-2007. I'll be away on holiday 05-08 October 2007. I'll be back on 09 October 2007 (Tuesday). Pathologists: I will process your requests when I return. If urgent, please forward your email to Henry_Kyaw@hsa.gov.sg _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Beatrice.Debrosse-Serra <@t> pfizer.com Thu Oct 4 13:19:34 2007 From: Beatrice.Debrosse-Serra <@t> pfizer.com (Debrosse-Serra, Beatrice) Date: Thu Oct 4 13:19:54 2007 Subject: [Histonet] Bone saw In-Reply-To: Message-ID: <8404DFBED5207B4B8E5EEF4332CEEA53044E56DC@lajamrexm01.amer.pfizer.com> Also try www.buehler.com Good luck! Bea Beatrice DeBrosse-Serra Pfizer Global Research & Development CB4, 2150 10646 Science Center Drive San Diego, CA 92121 Phone# 858-622-5986 Fax# 858-678-8290 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Orr, Rebecca Sent: Thursday, October 04, 2007 11:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bone saw Hi Jackie, Check out http://www.mar-med.com/bonesaw.html We use their cryovac hoses. They are based near Cleveland OH. Hope this helps. Is it Friday yet? Becky Orr CLA,HT(ASCP)QIHC Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From schaundrawalton <@t> yahoo.com Thu Oct 4 13:23:02 2007 From: schaundrawalton <@t> yahoo.com (Schaundra Walton) Date: Thu Oct 4 13:23:06 2007 Subject: [Histonet] Re: Space for Sectioning Message-ID: <977145.5596.qm@web58911.mail.re1.yahoo.com> We are in the process of building a new lab ourselves. Each of our new cutting stations will be L shaped and has 11' 6" of counter space with overhead storage, task lighting, and tackable surfaces. This is considerably larger than our current situation. Hope this helps some. Schaundra Walton BS HTL(ASCP) Swedish American Hospital 1401 E. State St. Rockford, IL 61104 --------------------------------- Fussy? Opinionated? Impossible to please? Perfect. Join Yahoo!'s user panel and lay it on us. From mpence <@t> grhs.net Thu Oct 4 14:35:52 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Oct 4 14:36:09 2007 Subject: [Histonet] antibodies In-Reply-To: <4704F551.3CA1.003C.0@hollandhospital.org> Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C746@IS-E2K3.grhs.net> A company on the west coast has such an antibody Zeta Corporation www.zeta-corp.com 888-355-2053 I have used the antibodies in the past. They work fine. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lisa Brenner Sent: Thursday, October 04, 2007 1:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] antibodies Hello, We have a pathologist who would like a triple stain. p63, high molecular weight CK, and racemase. Has anyone used this? Where do you find it? Please any information you could give me would be greatly appreciated. Lisa Brenner HTL (ASCP) Histology Technical Consultant Holland Hospital phone: (616)394-3184 lisab@hollandhospital.org Confidentiality Notice: The information contained in this e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information, or Protected Health Information as such term is defined under the Health Insurance Portability and Accountability Act of 1996 (HIPAA). Any unauthorized review, use, disclosure, copying or distribution is prohibited and may be unlawful. If you believe you have received this e-mail in error, please contact the sender by reply e-mail and delete all copies of the original message, including attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From arunams <@t> interchange.ubc.ca Thu Oct 4 15:34:05 2007 From: arunams <@t> interchange.ubc.ca (Aruna Somasiri) Date: Thu Oct 4 15:34:22 2007 Subject: [Histonet] JB-4 and MOVAT Message-ID: <15430869.27841191530045611.JavaMail.myubc2@handel.my.ubc.ca> Hi Everyone, I am trying to stain JB-4 embeded sections with movats and sections keep on falling off. Does any one know how to fix this problem of have a modified protocol I can use. Thanks a lot for your help. Regards aruna From Margaret.Perry <@t> sdstate.edu Thu Oct 4 16:48:30 2007 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Thu Oct 4 16:48:42 2007 Subject: [Histonet] used equipment dealers Message-ID: We are looking for a reputable used equipment dealer. Do you have any suggestions? Has anyone used Cambridge Scientific? Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 From failm <@t> musc.edu Thu Oct 4 17:47:59 2007 From: failm <@t> musc.edu (Mildred Fail) Date: Thu Oct 4 16:55:00 2007 Subject: [Histonet] antibodies Message-ID: biocare, though they changed the cytokeratin, the p63 and racmase are the same. The cytokeratin and p63 are stained with DAB since one is cytoplasmic, the other nuclear, it's easy to tell the difference, the racemase is stained red. Rena Fail Rena Fail >>> "Lisa Brenner" 10/04/07 14:15 PM >>> Hello, We have a pathologist who would like a triple stain. p63, high molecular weight CK, and racemase. Has anyone used this? Where do you find it? Please any information you could give me would be greatly appreciated. Lisa Brenner HTL (ASCP) Histology Technical Consultant Holland Hospital phone: (616)394-3184 lisab@hollandhospital.org Confidentiality Notice: The information contained in this e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information, or Protected Health Information as such term is defined under the Health Insurance Portability and Accountability Act of 1996 (HIPAA). Any unauthorized review, use, disclosure, copying or distribution is prohibited and may be unlawful. If you believe you have received this e-mail in error, please contact the sender by reply e-mail and delete all copies of the original message, including attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Thu Oct 4 23:29:21 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu Oct 4 23:29:28 2007 Subject: [Histonet] Re: Space for Sectioning In-Reply-To: <977145.5596.qm@web58911.mail.re1.yahoo.com> References: <977145.5596.qm@web58911.mail.re1.yahoo.com> Message-ID: on average, how many sectioning spaces do any of you have? we have one paraffin sectioning space (2 feet depth by one slide warmer length) and one cryostat sectioning place (size of cryostat CM3050).. again, just wondering out of curiosity how big processing labs can get (reminder, i work in academics, so we need to prove we need our space, limited as it is, or we lose it) also, when i volunteered in a pathology lab in a hospital as an undergraduate (circa 1997, EONS ago), i didn't see a cryostat anywhere, just paraffin processing instruments. has this changed? it seems for large volumes of processing, paraffin would be better, yet it's less sensitive to certain antibodies due to the melting point of paraffin. emily ps sorry to ask silly questions, i never thought about paraffin vs cryo-sectioning in large volumes until now. it interests me to know which is better for pathology, or whatever histonet people might use most :) -- Yog-Sothoth knows the gate. Yog-Sothoth is the gate. Yog-Sothoth is the key and guardian of the gate. Past, present, future, all are one in Yog-Sothoth. He knows where the Old Ones broke through of old, and where They shall break through again. From garth <@t> apollosci.co.za Fri Oct 5 01:18:34 2007 From: garth <@t> apollosci.co.za (Garth Jerome) Date: Fri Oct 5 01:19:05 2007 Subject: [Histonet] Bone Saws In-Reply-To: Message-ID: <000001c80717$8f6767a0$7700a8c0@jhb.apollosci.co.za> Hello Jackie After struggling for many years with vices and hacksaws and thick rubber gloves....I convinced our laboratory director to purchase an electric saw for cutting bone. We purchased a used Beuhler Isomet saw. This saw is designed for metallurgy, but it works beautifully! A new one is a little expensive, but if you do a lot of bone, then it may be a worthwhile investment. The saw has an enclosed housing to prevent aerosols, which is great. Regards Garth Jerome Apollo Scientific cc Telephone : 27-11-466 7666 Facsimile : 27-11-466 7672 Personal facsimile : 086 660 0539 Cell phone : 084 504 1101 Email : garth@apollosci.co.za -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: 04 October 2007 05:19 PM To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] Bone Saws Does anyone have a suggestion for a good electric bone saw for histology? My intent is to trim un-decalcified bone to 3mm to facilitate/speed up decalcification. Are there any rules governing bone dust? I remember years ago, a pathologist complaining that anything but a hacksaw creating a burning artifact in the bone. Any thoughts on this? Jackie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Andrew.Prior <@t> Smith-Nephew.com Fri Oct 5 02:43:09 2007 From: Andrew.Prior <@t> Smith-Nephew.com (Prior, Andrew) Date: Fri Oct 5 02:43:19 2007 Subject: [Histonet] RE: Histonet Bone Saws In-Reply-To: <20071004170053.4BAE3D512547@spam.smith-nephew.com> References: <20071004170053.4BAE3D512547@spam.smith-nephew.com> Message-ID: <7028DD15E14FDC4DB1F3C5AF8735AF370398F943@EHS021.wound.san> We use the Isomet Low Speed Saw from Beuhler. It's easy and safe to use and causes little artefact in the bone. It has a water reservoir so there is little dust produced, though it does splash a little sometimes. Andrew Prior Histologist Smith &Nephew Research Centre York Science Park Heslington York YO10 5DF UK Andrew.Prior@smith-nephew.com 01904 824022 ------------------------------ >Message: 17 >Date: Thu, 4 Oct 2007 10:18:31 -0500 >From: Jackie M O'Connor >Subject: [Histonet] Bone Saws >To: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu >Message-ID: >Content-Type: text/plain; charset="US-ASCII" >Does anyone have a suggestion for a good electric bone saw for histology? >My intent is to trim un-decalcified bone to 3mm to facilitate/speed up >decalcification. >Are there any rules governing bone dust? I remember years ago, a >pathologist complaining that anything but a hacksaw creating a burning >artifact in the bone. >Any thoughts on this? >>Jackie ------------------------------ ************************** Confidentiality. This electronic transmission is strictly confidential to Smith & Nephew and intended solely for the addressee. 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Smith & Nephew UK Limited Registered in England and Wales No.4421171 with registered office at 15 Adam Street, London WC2N 6LA From failm <@t> musc.edu Fri Oct 5 07:15:24 2007 From: failm <@t> musc.edu (Mildred Fail) Date: Fri Oct 5 07:16:55 2007 Subject: [Histonet] Re: Space for Sectioning Message-ID: With The addition of automatic stainers, demos for automatic stainers, carboys for automatic stainers, counterspace and knee space keep shrinking in our lab.4 inches either side of the microtome and the width of the waterbath is all for the counterspace, As for a place for your feet and legs, you can balance your feet on either carboys, waste basket , or cardboard boxes storing run maps. ;) Rena Fail >>> "Emily Sours" 10/05/07 12:29AM >>> on average, how many sectioning spaces do any of you have? we have one paraffin sectioning space (2 feet depth by one slide warmer length) and one cryostat sectioning place (size of cryostat CM3050).. again, just wondering out of curiosity how big processing labs can get (reminder, i work in academics, so we need to prove we need our space, limited as it is, or we lose it) also, when i volunteered in a pathology lab in a hospital as an undergraduate (circa 1997, EONS ago), i didn't see a cryostat anywhere, just paraffin processing instruments. has this changed? it seems for large volumes of processing, paraffin would be better, yet it's less sensitive to certain antibodies due to the melting point of paraffin. emily ps sorry to ask silly questions, i never thought about paraffin vs cryo-sectioning in large volumes until now. it interests me to know which is better for pathology, or whatever histonet people might use most :) -- Yog-Sothoth knows the gate. Yog-Sothoth is the gate. Yog-Sothoth is the key and guardian of the gate. Past, present, future, all are one in Yog-Sothoth. He knows where the Old Ones broke through of old, and where They shall break through again. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Oct 5 07:27:52 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 5 07:27:58 2007 Subject: [Histonet] Re: Space for Sectioning In-Reply-To: Message-ID: <118594.31850.qm@web61219.mail.yahoo.com> Rena: If your answer is NOT "just a good joke", you are facing a serious ergonomic problem, with some potential claustrofobic consequences. Ren? J. Mildred Fail wrote: With The addition of automatic stainers, demos for automatic stainers, carboys for automatic stainers, counterspace and knee space keep shrinking in our lab.4 inches either side of the microtome and the width of the waterbath is all for the counterspace, As for a place for your feet and legs, you can balance your feet on either carboys, waste basket , or cardboard boxes storing run maps. ;) Rena Fail >>> "Emily Sours" 10/05/07 12:29AM >>> on average, how many sectioning spaces do any of you have? we have one paraffin sectioning space (2 feet depth by one slide warmer length) and one cryostat sectioning place (size of cryostat CM3050).. again, just wondering out of curiosity how big processing labs can get (reminder, i work in academics, so we need to prove we need our space, limited as it is, or we lose it) also, when i volunteered in a pathology lab in a hospital as an undergraduate (circa 1997, EONS ago), i didn't see a cryostat anywhere, just paraffin processing instruments. has this changed? it seems for large volumes of processing, paraffin would be better, yet it's less sensitive to certain antibodies due to the melting point of paraffin. emily ps sorry to ask silly questions, i never thought about paraffin vs cryo-sectioning in large volumes until now. it interests me to know which is better for pathology, or whatever histonet people might use most :) -- Yog-Sothoth knows the gate. Yog-Sothoth is the gate. Yog-Sothoth is the key and guardian of the gate. Past, present, future, all are one in Yog-Sothoth. He knows where the Old Ones broke through of old, and where They shall break through again. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Boardwalk for $500? In 2007? Ha! Play Monopoly Here and Now (it's updated for today's economy) at Yahoo! Games. From failm <@t> musc.edu Fri Oct 5 07:56:56 2007 From: failm <@t> musc.edu (Mildred Fail) Date: Fri Oct 5 07:58:50 2007 Subject: [Histonet] Re: Space for Sectioning Message-ID: My answer is........NOT "just a good joke" Rena Fail >>> Rene J Buesa 10/05/07 08:27AM >>> Rena: If your answer is NOT "just a good joke", you are facing a serious ergonomic problem, with some potential claustrofobic consequences. Ren? J. Mildred Fail wrote: With The addition of automatic stainers, demos for automatic stainers, carboys for automatic stainers, counterspace and knee space keep shrinking in our lab.4 inches either side of the microtome and the width of the waterbath is all for the counterspace, As for a place for your feet and legs, you can balance your feet on either carboys, waste basket , or cardboard boxes storing run maps. ;) Rena Fail >>> "Emily Sours" 10/05/07 12:29AM >>> on average, how many sectioning spaces do any of you have? we have one paraffin sectioning space (2 feet depth by one slide warmer length) and one cryostat sectioning place (size of cryostat CM3050).. again, just wondering out of curiosity how big processing labs can get (reminder, i work in academics, so we need to prove we need our space, limited as it is, or we lose it) also, when i volunteered in a pathology lab in a hospital as an undergraduate (circa 1997, EONS ago), i didn't see a cryostat anywhere, just paraffin processing instruments. has this changed? it seems for large volumes of processing, paraffin would be better, yet it's less sensitive to certain antibodies due to the melting point of paraffin. emily ps sorry to ask silly questions, i never thought about paraffin vs cryo-sectioning in large volumes until now. it interests me to know which is better for pathology, or whatever histonet people might use most :) -- Yog-Sothoth knows the gate. Yog-Sothoth is the gate. Yog-Sothoth is the key and guardian of the gate. Past, present, future, all are one in Yog-Sothoth. He knows where the Old Ones broke through of old, and where They shall break through again. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Boardwalk for $500? In 2007? Ha! Play Monopoly Here and Now (it's updated for today's economy) at Yahoo! Games. From mohs76009 <@t> yahoo.com Fri Oct 5 08:20:05 2007 From: mohs76009 <@t> yahoo.com (Matt Bancroft) Date: Fri Oct 5 08:20:11 2007 Subject: [Histonet] used equipment dealers In-Reply-To: Message-ID: <619890.4177.qm@web63409.mail.re1.yahoo.com> I set up a lab using Histotronix. All of their refurbished equipment has been re-painted and they look new. The owner is Tom Buck and his number is 712-249-0490 and the website is www.histotronix.com. I highly recommend him. Matt Bancroft HT(ASCP) "Perry, Margaret" wrote: We are looking for a reputable used equipment dealer. Do you have any suggestions? Has anyone used Cambridge Scientific? Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Need a vacation? Get great deals to amazing places on Yahoo! Travel. From cmiller <@t> physlab.com Fri Oct 5 11:24:34 2007 From: cmiller <@t> physlab.com (Cheri Miller) Date: Fri Oct 5 11:24:46 2007 Subject: [Histonet] used equipment dealers In-Reply-To: <619890.4177.qm@web63409.mail.re1.yahoo.com> References: <619890.4177.qm@web63409.mail.re1.yahoo.com> Message-ID: <006a01c8076c$3ac99150$3402a8c0@plab.local> We use Tom Buck/ Histrionics too. He also maintains all our equipment. He never fails to help me with whatever I need. I highly recommend him and his crew..... Cheri Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matt Bancroft Sent: Friday, October 05, 2007 8:20 AM To: Perry, Margaret; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] used equipment dealers I set up a lab using Histotronix. All of their refurbished equipment has been re-painted and they look new. The owner is Tom Buck and his number is 712-249-0490 and the website is www.histotronix.com. I highly recommend him. Matt Bancroft HT(ASCP) "Perry, Margaret" wrote: We are looking for a reputable used equipment dealer. Do you have any suggestions? Has anyone used Cambridge Scientific? Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Need a vacation? Get great deals to amazing places on Yahoo! Travel. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From rosenfeldtek <@t> hotmail.com Fri Oct 5 11:37:58 2007 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Fri Oct 5 11:38:04 2007 Subject: [Histonet] Bone proliferation In-Reply-To: <572057D3BDD52A46BD05BC6DA50686110CAED2@MSGEBE22.mfad.mfroot.org> References: <572057D3BDD52A46BD05BC6DA50686110CAED2@MSGEBE22.mfad.mfroot.org> Message-ID: Why not PCNA? Jerry L. Ricks Research Scientist U.W. Medicine at South Lake Union 815 Mercer Street Seattle, WA 98109 (206)-685-7190> Date: Wed, 3 Oct 2007 09:59:35 -0500> From: Herrick.James@mayo.edu> To: histonet@lists.utsouthwestern.edu> Subject: [Histonet] Bone proliferation> > Good morning everyone,> > I have been asked a very general question in regards to a good stain> that can be used to show bone proliferation, without any association to> BRDU. I don't have an answer but was wondering if any of you might.> Thank you very much.> > Jim> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Peek-a-boo FREE Tricks & Treats for You! http://www.reallivemoms.com?ocid=TXT_TAGHM&loc=us From jmahoney <@t> alegent.org Fri Oct 5 11:38:28 2007 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Fri Oct 5 11:38:42 2007 Subject: [Histonet] used equipment dealers In-Reply-To: <006a01c8076c$3ac99150$3402a8c0@plab.local> References: <619890.4177.qm@web63409.mail.re1.yahoo.com> <006a01c8076c$3ac99150$3402a8c0@plab.local> Message-ID: <470622340200003C0001CDCD@gwia.alegent.org> Same here, Tom and his crew are the best. They will work with you and really understand the issues we face when equipment is down or not working properly. Jan Omaha From kalschev <@t> svm.vetmed.wisc.edu Fri Oct 5 12:05:37 2007 From: kalschev <@t> svm.vetmed.wisc.edu (Vicki Kalscheur) Date: Fri Oct 5 12:05:42 2007 Subject: [Histonet] Hard Tissue Committee Meeting @NSH 2007' Message-ID: <000a01c80771$f31e5790$c5d76880@vetmed.wisc.edu> Dear members and interested attendees: Please join us on Saturday, October 27th, 2007 from 5:30 - 6:30 p.m. in Denver, Colorado as we convene at NSH's 33rd Annual Symposium located in the Colorado Convention Center. The meeting room number will be posted at the Hard Tissue Display Table. I look forward to seeing many of you! Best regards, Vicki Kalscheur, Committee Chair From TJJ <@t> Stowers-Institute.org Fri Oct 5 12:17:33 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Fri Oct 5 12:17:55 2007 Subject: [Histonet] Re: Histrionics??? Message-ID: I'm not sure Tom Buck really had this in mind when he named his company "Histotronix". But this did give me a good chuckle, I'm wondering if Spell Check changed this for you. Priceless! >>Message: 19 Date: Fri, 5 Oct 2007 11:24:34 -0500 From: "Cheri Miller" Subject: RE: [Histonet] used equipment dealers To: "'Matt Bancroft'" , "'Perry, Margaret'" , Message-ID: <006a01c8076c$3ac99150$3402a8c0@plab.local> Content-Type: text/plain; charset="us-ascii" We use Tom Buck/ Histrionics too. He also maintains all our equipment. He never fails to help me with whatever I need. I highly recommend him and his crew..... Cheri Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052<< Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From RSRICHMOND <@t> aol.com Fri Oct 5 12:38:25 2007 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Fri Oct 5 12:38:30 2007 Subject: [Histonet] Re: Bone Saws Message-ID: That Buehler Isomet low speed saw may be seen at: http://www.buehler.com/productinfo/saws.htm I've never seen one of these, and don't know what they cost. What's its footprint - how much table space will it take up? A continuing medical education program I did about eight years ago recommended an electric scroll saw, $100 to $200 at major hardware outlets. I've never seen one in use, but one problem with it is its large footprint. The electric band saw is the queen of battle, but much too dangerous for routine use. I've liked MOPEC's "Sawbones" - a double bladed hacksaw with a vise - but at $500 it's not a toy the red haired stepchild can have. Few pathology services I work in possess any hand saws, but usually have a Stryker oscillating saw left over from the days when they did autopsies. I won't use a Stryker saw for loose specimens, but many people do. I still use the Civil War era Satterlee amputation saw when I can get one, and a hardware store hacksaw when I can't. (I've twice seen Civil War hospital re-enactments with a Satterlee saw as part of their kit. Chrome plated, of course. Bob Richmond Samurai Pathologist and antiquated sawbones Knoxville TN From crochieresteve <@t> aol.com Fri Oct 5 12:58:43 2007 From: crochieresteve <@t> aol.com (crochieresteve@aol.com) Date: Fri Oct 5 12:59:02 2007 Subject: [Histonet] prostate triple stain Message-ID: <8C9D59080D78314-1968-5C19@WEBMAIL-MB14.sysops.aol.com> I use the PIN-4 cocktail from Biocare Medical. It works very well.Catalog # PPM225DSAA You will also need the double stain detection kit. I use the automated version on their stainer, but there's a manual kit available as well. Call them 800-799-9499 Steve Crochiere HT(ASCP) Histology Supervisor New England Pathology Associates Springfield, MA ________________________________________________________________________ Email and AIM finally together. You've gotta check out free AOL Mail! - http://mail.aol.com From froyer <@t> bitstream.net Fri Oct 5 13:41:24 2007 From: froyer <@t> bitstream.net (Ford Royer) Date: Fri Oct 5 13:41:46 2007 Subject: [Histonet] Re: Bone Saws In-Reply-To: References: Message-ID: <003f01c8077f$55168780$7701a80a@Ford> The footprint of Isomet units that I have dealt with are approximately 24-26" square and 12-14" high with the lid closed. They do take up a fair amount of bench space, but they can be set on a separate table (i.e. 36" x 36") which most of my customers have done. This works well to free up bench space and also allows for "head room" when the lid is open... which requires another couple of feet above the instrument. They also have some weight to them... ~ 50 lbs. Ford M. Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL:? 612-839-1046 Phone:? 763-542-8725 Fax:? 763-546-4830 eMail:? clinicallab@minnesotamedical.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Friday, October 05, 2007 12:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Bone Saws That Buehler Isomet low speed saw may be seen at: http://www.buehler.com/productinfo/saws.htm I've never seen one of these, and don't know what they cost. What's its footprint - how much table space will it take up? A continuing medical education program I did about eight years ago recommended an electric scroll saw, $100 to $200 at major hardware outlets. I've never seen one in use, but one problem with it is its large footprint. The electric band saw is the queen of battle, but much too dangerous for routine use. I've liked MOPEC's "Sawbones" - a double bladed hacksaw with a vise - but at $500 it's not a toy the red haired stepchild can have. Few pathology services I work in possess any hand saws, but usually have a Stryker oscillating saw left over from the days when they did autopsies. I won't use a Stryker saw for loose specimens, but many people do. I still use the Civil War era Satterlee amputation saw when I can get one, and a hardware store hacksaw when I can't. (I've twice seen Civil War hospital re-enactments with a Satterlee saw as part of their kit. Chrome plated, of course. Bob Richmond Samurai Pathologist and antiquated sawbones Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMEarle <@t> CapeCodHealth.org Fri Oct 5 13:48:29 2007 From: PMEarle <@t> CapeCodHealth.org (Earle, Paul M.) Date: Fri Oct 5 13:48:34 2007 Subject: [Histonet] Bone Cutting Message-ID: <29688FCA0750AD4FB55B18D961EB91CC10172D@cchex2.cchdomain1.capecodhealth.org> After many years of trying manual saws and electric saws (Stryker, band, etc) with a variety of adaptations (from meatball tongs to handmade templates) to hold femoral heads, ect....We went back to what a fellow PA (thanks Russ J.) brought to our institution, and to what an older pathologist once showed me. We split bones with a SHARP heavy duty knife (similar to the old autopsy knives that are thick, and which look like a wedge.) This is used with a heavy metal mallet. IT MUST BE DONE ON A SOLID SURFACE. We use a thick triangular piece of plexiglass, with two short sides and a base constructed to fit on the corner of our Gross Lab, and a thick rubber adhesive mat over this. With practice, one can get sections that are thin enough to be cassetted and decalcified without further handling. You also eliminate the bone dust in your sections and in the air. Paul M. Earle M.S., P.A. (ASCP) Anatomic Pathology Supervisor Pathology Department Falmouth Hospital 100 Ter Heun Drive Falmouth, MA 02540 508-548-5300 x73488 <> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org From kzhong888 <@t> yahoo.com Fri Oct 5 17:51:07 2007 From: kzhong888 <@t> yahoo.com (dfs dsaf) Date: Fri Oct 5 17:51:10 2007 Subject: [Histonet] RE: Buehler Isomet Plus Saw Message-ID: <626273.88773.qm@web52904.mail.re2.yahoo.com> Hello histonetters: I see that recently there has been people looking for the Buehler Isomet Plus Saw. I recently saw one on LABX when i googled Buehler Isomet Plus Saw. it looks good and has manual asking price is $4500. and the person's email address is Pantur@aol.com. I hope this helps. thank you Kirk --------------------------------- Fussy? Opinionated? Impossible to please? Perfect. Join Yahoo!'s user panel and lay it on us. From JMacDonald <@t> mtsac.edu Sat Oct 6 15:16:06 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Sat Oct 6 15:16:04 2007 Subject: [Histonet] Re: Space for Sectioning In-Reply-To: Message-ID: Our student histology laboratory has 24 work stations. Each work station is four feet wide and a little over two feet deep. There is a small return to the left for the water bath for correct ergonomics. Our cryostat and tissue processors are housed in a separate room off of the lab. Our grossing stations and flammable cabinet are also in this room. In the main lab we have 6 embedding centers along one wall. We also have four H&E staining set-ups with snorkel ventilation and sinks for each set-up. We have two large fume hoods that are used for coverslipping. Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu "Emily Sours" Sent by: histonet-bounces@lists.utsouthwestern.edu 10/04/2007 09:29 PM To histonet@lists.utsouthwestern.edu cc Subject Re: [Histonet] Re: Space for Sectioning on average, how many sectioning spaces do any of you have? we have one paraffin sectioning space (2 feet depth by one slide warmer length) and one cryostat sectioning place (size of cryostat CM3050).. again, just wondering out of curiosity how big processing labs can get (reminder, i work in academics, so we need to prove we need our space, limited as it is, or we lose it) also, when i volunteered in a pathology lab in a hospital as an undergraduate (circa 1997, EONS ago), i didn't see a cryostat anywhere, just paraffin processing instruments. has this changed? it seems for large volumes of processing, paraffin would be better, yet it's less sensitive to certain antibodies due to the melting point of paraffin. emily ps sorry to ask silly questions, i never thought about paraffin vs cryo-sectioning in large volumes until now. it interests me to know which is better for pathology, or whatever histonet people might use most :) -- Yog-Sothoth knows the gate. Yog-Sothoth is the gate. Yog-Sothoth is the key and guardian of the gate. Past, present, future, all are one in Yog-Sothoth. He knows where the Old Ones broke through of old, and where They shall break through again. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mickie25 <@t> netzero.net Sat Oct 6 21:11:29 2007 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Sat Oct 6 21:11:53 2007 Subject: [Histonet] RE: Hey! In-Reply-To: <8C9D57B766C1A82-D18-52C5@WEBMAIL-DC10.sysops.aol.com> References: <8C9D57B766C1A82-D18-52C5@WEBMAIL-DC10.sysops.aol.com> Message-ID: Hi Susan, It sounds like you did well. Did you get any indication that Dr. Sawyer would want you or me to come back in the near future? I don't want to set you up for something else if she has a need. Yes, that was good to order them a Belair catalogue. Were they using Gills II or III stain? I will send you a protocol I have found to work very well for Mohs. Best, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net _____ From: historsd@aol.com [mailto:historsd@aol.com] Sent: Friday, October 05, 2007 8:28 AM To: mickie25@netzero.net Subject: Hey! Hi Mickie, Just to touch base. I ended up training two people. One of the girls was a PA and had a little experience. She caught on very quickly, asked the correct questions and cut good sections with me looking over her shoulder. The only troubleshooting I managed to get to was plastering defects and placement of epi so it is perpendicular to knife. The other girl is the aesthetition (I know I spelled that wrong). She was definately more unsure of herself but did manage to complete the entire process, embedding through sectioning and she did cut some nice sections. We even managed a little microscopic examination. Dr. Sawyer has tried to use histotechs with no MOHS training. She has only done 26 cases. I mentioned your suggestion of having a trained MOHS tech look over a shoulder for 4 - 5 days, she said she only does MOHS one day a week. They are also having a great deal of difficulty with their stain. Most of the stains and reagents are for paraffin. I ordered them a Belair catalog. Was that ok? or should you have done that? Dr. Sawyer was very happy with the slides. We finished 7 cases the day I cut and more than once I heard the comment that they had never been finished so early in the day. Thanks for the check. I definately like the work and the challenge. Do you utilize only Belair products? Should I keep a few of these catalogs with me? Sorry so many ??? Just want to do right by you. I appreciate the work. Let me know about Tenn. Susan _____ Email and AIM finally together. You've gotta check out free AOL Mail ! From pcrean222 <@t> gmail.com Sun Oct 7 18:28:43 2007 From: pcrean222 <@t> gmail.com (Pat Crean) Date: Sun Oct 7 18:28:58 2007 Subject: [Histonet] ventana carousel...free Message-ID: ---------- Forwarded message ---------- From: Terri Braud Date: Oct 2, 2007 11:16 AM Subject: ad To: pcrean222@gmail.com Free Ventana! Our lab has graduated to a Benchmark XL and has a New In Box reagent carousel for the old ES system. I'm not sure if it is the same as the Nexus or not, but if you want it, please send me an email with your Fed Ex or UPS account number and I will ship it to you. First come, first serve. *Terri L. Braud, HT(ASCP)* *Anatomic Pathology Supv.* *Laboratory, Holy Redeemer Hospital* *1648 Huntingdon Pike* *Meadowbrook, PA 19046* *(215) 938-3689* --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From Mary.Cheles <@t> leica-microsystems.com Mon Oct 8 05:45:19 2007 From: Mary.Cheles <@t> leica-microsystems.com (Mary.Cheles@leica-microsystems.com) Date: Mon Oct 8 05:45:51 2007 Subject: [Histonet] Job Opportunity with Leica Microsystems Message-ID: Dear Histonetters, Leica Microsystems Inc., Biosystems Division, is an internationally renowned developer and manufacturer of laboratory instrumentation for histopathology. Leica prides itself on providing the most innovative solutions to our customers? needs for specimen handling, preparation and analysis. We have an exciting opportunity in our Technical Assistance Center (TAC) for an Applications Specialist that will provide technical phone support and product training on Leica?s routine histology and immunohistochemistry products. The position is based in Bannockburn, Illinois office?s new Customer Support Laboratory. Candidate should possess excellent communication, customer relations, teambuilding and organizational skills. Bachelor's degree preferred with a minimum of two years histology, immunohistochemistry and/or ultramicrotomy experience. We offer great benefits (including medical, dental, vision, prescription, long term care, life insurance, STD, LTD, 401K and a retirement savings plan), excellent salary, vacation and holiday schedule. For consideration, forward your resume and salary requirements referencing job code BSD-AS to: Leica Microsystems, Inc. HR Dept- 2345 Waukegan Rd., Bannockburn, IL 60015. FAX to 847-236-3035. Email: beth.farrow@leica-microsystems.com. Please consider joining our expanding team of professionals. Mary Cheles Director, Technical Services Leica Microsystems, Biosystems Division Office: 847-317-5916 Email: mary.cheles@leica-microsystems.com ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From barry_m <@t> ozemail.com.au Mon Oct 8 07:20:55 2007 From: barry_m <@t> ozemail.com.au (Barry Madigan) Date: Mon Oct 8 07:21:20 2007 Subject: [Histonet] prostate triple stain In-Reply-To: <8C9D59080D78314-1968-5C19@WEBMAIL-MB14.sysops.aol.com> References: <8C9D59080D78314-1968-5C19@WEBMAIL-MB14.sysops.aol.com> Message-ID: <000001c809a5$b0440240$10cc06c0$@com.au> We have been using an in house cocktail of P63, high molecular weight cytokeratin and AMACR (P504S) for some time now with good results on prostate specimens. All antibodies obtained from DAKO. P63/34BE12 with Polymer peroxidase and DAB. P504S with Polymer Alkaline Phosphatase with Fast Red. Completely done on a Vision BioSystem BondMax Immunostainer. Barry Madigan Immunohistochemistry Pathology Queensland Royal Brisbane Hospital Campus Australia -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of crochieresteve@aol.com Sent: Saturday, 6 October 2007 3:59 AM To: histonet@pathology.swmed.edu Subject: [Histonet] prostate triple stain I use the PIN-4 cocktail from Biocare Medical. It works very well.Catalog # PPM225DSAA You will also need the double stain detection kit. I use the automated version on their stainer, but there's a manual kit available as well. Call them 800-799-9499 Steve Crochiere HT(ASCP) Histology Supervisor New England Pathology Associates Springfield, MA ________________________________________________________________________ Email and AIM finally together. You've gotta check out free AOL Mail! - http://mail.aol.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thisisann <@t> aol.com Mon Oct 8 08:01:47 2007 From: thisisann <@t> aol.com (thisisann@aol.com) Date: Mon Oct 8 08:19:17 2007 Subject: [Histonet] Peloris Message-ID: <8C9D7C284D4CB59-16D4-925@Webmail-mg02.sysops.aol.com> If anyone uses the Peloris Tissue processor, can you tell me what type of processing schedule (Xylene free) you use for Prostate and G.I. biopsies which have been fixed for approx. 5-8 hours in 10% Formalin. Also, how did you perform your validation for this processing schedule? ________________________________________________________________________ Email and AIM finally together. You've gotta check out free AOL Mail! - http://mail.aol.com From CBark <@t> memorialcare.org Mon Oct 8 09:31:11 2007 From: CBark <@t> memorialcare.org (Christine Bark) Date: Mon Oct 8 09:31:53 2007 Subject: [Histonet] Laboratory microwave Message-ID: Good morning Histonetters, I know this has been discussed before but I need some help finding a laboratory microwave for my special stains. I currently have a household microwave under a fume-hood. Vendors are welcome to respond. Thank you, Christine Bark Senior Histotech, Pathology Saddleback Memorial Medical Center 949-452-3548 cbark@memorialcare.org ______________________________________________________________________________ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Anna.Inman <@t> stmarygj.org Mon Oct 8 09:47:17 2007 From: Anna.Inman <@t> stmarygj.org (Inman, Anna) Date: Mon Oct 8 09:47:35 2007 Subject: [Histonet] Cell block Message-ID: <2925AE271EAAD440AF48FCCEB8002D090542F837@smgmail01.smgj.sclhs.net> Hello - For those you who perform cytology cell blocks - if the fluid (pleural, peritoneal, pericardial) has a "clot" (mucous, tumor or whatever) do you only submit the clot for cell block or do you also spin fluid down and add whatever button you may have, after decanting, to the clot for processing? Thank you Anna CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From sheila_adey <@t> hotmail.com Mon Oct 8 10:04:42 2007 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Mon Oct 8 10:04:55 2007 Subject: [Histonet] Cell block In-Reply-To: <2925AE271EAAD440AF48FCCEB8002D090542F837@smgmail01.smgj.sclhs.net> References: <2925AE271EAAD440AF48FCCEB8002D090542F837@smgmail01.smgj.sclhs.net> Message-ID: Our Dr.s have told us that the spun down sediment is much more beneficial than the clot in the bottle. Sheila Adey HT MLTPort Huron HospitalMichigan> Date: Mon, 8 Oct 2007 08:47:17 -0600> From: Anna.Inman@stmarygj.org> To: histonet@lists.utsouthwestern.edu> Subject: [Histonet] Cell block> > Hello - For those you who perform cytology cell blocks - if the fluid> (pleural, peritoneal, pericardial) has a "clot" (mucous, tumor or> whatever) do you only submit the clot for cell block or do you also spin> fluid down and add whatever button you may have, after decanting, to the> clot for processing? > > Thank you > Anna > > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.> > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Are you ready for Windows Live Messenger Beta 8.5 ? Get the latest for free today! http://entertainment.sympatico.msn.ca/WindowsLiveMessenger From mpence <@t> grhs.net Mon Oct 8 10:18:46 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Mon Oct 8 10:18:58 2007 Subject: [Histonet] CK7 Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C74C@IS-E2K3.grhs.net> Need some help: Does anyone know the molecular weight that CK7 marks? Is it high or low? Thanks, Mike From mpence <@t> grhs.net Mon Oct 8 10:50:01 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Mon Oct 8 10:50:15 2007 Subject: [Histonet] CK7 In-Reply-To: <661949901A768E4F9CC16D8AF8F2838CA1C74C@IS-E2K3.grhs.net> Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C74D@IS-E2K3.grhs.net> I have found this helpful site that might be of interest to you all. http://www.nordiqc.org/Epitopes/Cytokeratins/cytokeratins.htm Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Monday, October 08, 2007 10:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CK7 Need some help: Does anyone know the molecular weight that CK7 marks? Is it high or low? Thanks, Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bhewlett <@t> cogeco.ca Mon Oct 8 11:34:06 2007 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Mon Oct 8 11:34:21 2007 Subject: [Histonet] CK7 References: <661949901A768E4F9CC16D8AF8F2838CA1C74C@IS-E2K3.grhs.net> Message-ID: <002801c809c9$0b95c3e0$6500a8c0@mainbox> Mike, CK7 is a 54 kDa, LMW cytokeratin. Bryan ----- Original Message ----- From: "Mike Pence" To: Sent: Monday, October 08, 2007 11:18 AM Subject: [Histonet] CK7 Need some help: Does anyone know the molecular weight that CK7 marks? Is it high or low? Thanks, Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From moran.elish <@t> gmail.com Mon Oct 8 11:37:48 2007 From: moran.elish <@t> gmail.com (Moran Elishmereni) Date: Mon Oct 8 11:37:59 2007 Subject: [Histonet] can I leave IHC sections overnight after first staining? Message-ID: <4a722ef70710080937o1e8e2ffp6a2f0d735026edfd@mail.gmail.com> Hi Histonetters, Is it possible to leave sections (that were stained with primary Ab) overnight, and counterstain the day after? I am using DAB reaction. Can I leave it dry (or wet, in distilled water) overnight? Thanks, Moran -- Moran Elishmereni Department of Pharmacology and Experimental Therapeutics School of Pharmacy, Faculty of Medicine The Hebrew University of Jerusalem POB 12065 Jerusalem 91120, ISRAEL Tel: 972-2-675-8746 Fax: 972-2-675-8144 Email: moran.elish@gmail.com From mpence <@t> grhs.net Mon Oct 8 11:58:13 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Mon Oct 8 11:58:28 2007 Subject: [Histonet] can I leave IHC sections overnight after first staining? In-Reply-To: <4a722ef70710080937o1e8e2ffp6a2f0d735026edfd@mail.gmail.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C74E@IS-E2K3.grhs.net> Why would you do all the staining, then stop something that takes less the 10 more minutes to finish? Just asking. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Moran Elishmereni Sent: Monday, October 08, 2007 11:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] can I leave IHC sections overnight after first staining? Hi Histonetters, Is it possible to leave sections (that were stained with primary Ab) overnight, and counterstain the day after? I am using DAB reaction. Can I leave it dry (or wet, in distilled water) overnight? Thanks, Moran -- Moran Elishmereni Department of Pharmacology and Experimental Therapeutics School of Pharmacy, Faculty of Medicine The Hebrew University of Jerusalem POB 12065 Jerusalem 91120, ISRAEL Tel: 972-2-675-8746 Fax: 972-2-675-8144 Email: moran.elish@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Mon Oct 8 13:02:11 2007 From: tkngflght <@t> yahoo.com (Cheryl R. Kerry) Date: Mon Oct 8 13:02:14 2007 Subject: [Histonet] An idea that needs input--HT Continuing ed game--for free Message-ID: <009a01c82231$7b8e14b0$6a01a8c0@CHERYLSLAPTOP> Hi all--- I need your input. If you would pass this on to other histology folks for their input if they aren't on the net~I appreciate the help! I want to come up with a project to improve our overall understanding of our science, to work on creating an improved reputation as part of the clinical laboratory and help everyone have a better day--every day--at work. AND more importantly--to connect us as a community into a more-cohesive voice. THE BACKKGROUND FOR THIS PROJECT-- I am working on a grad class. I need a project to improve a community of which I am already a member without any focus on self-gain. I could pick my church or my daughter's school for this project--but that's playing way too small. I want to help in a bigger way. I LOVE what I do--I'm a histotech and a recruiter--and have read lots of discussion on inequities in education, experience, skills, understanding by histotechs (including me!) who do this work. Some of us are registered, some aren't, some are required to do continuing ed, some aren't. Some have made this a career, for some it's a job. I would like to come up with a format to provide accredited continuing ed that is FUN so it is used, increases our understanding of what we do and why--and lots of other good things. THE IDEA-- My first idea is a game or a contest that posts monthly with prizes and group interaction. Second would be a board game for your lab with new questions coming out quarterly or biannually (leave it open in the break room and play through the day). Another idea is to piggy-back our APNews newsletter with an article or a CE credit contributed from our peers with sponsored prized for completion (boring but practical). I need help with a couple of questions if you could respond to me directly off the net so I can correlate and architect this idea into something fun that we would WANT to play-- 1. Would you be interested in something like this if it is voluntary and FUN? 2. Do you have a other idea for me to try on instead? 3. Any ideas for format or distribution? 4. What could we call it? 5. Would you want to help? I know we're all busy--and I appreciate your time and contribution. I'll keep you posted on where this goes and will be asking for more guidance as the project gets off the ground. THANK YOU!!!!! Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab, one great tech at a time. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone From rjbuesa <@t> yahoo.com Mon Oct 8 13:31:54 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Oct 8 13:32:04 2007 Subject: [Histonet] Cell block In-Reply-To: <2925AE271EAAD440AF48FCCEB8002D090542F837@smgmail01.smgj.sclhs.net> Message-ID: <582869.73632.qm@web61217.mail.yahoo.com> Yes! Ren? J. "Inman, Anna" wrote: Hello - For those you who perform cytology cell blocks - if the fluid (pleural, peritoneal, pericardial) has a "clot" (mucous, tumor or whatever) do you only submit the clot for cell block or do you also spin fluid down and add whatever button you may have, after decanting, to the clot for processing? Thank you Anna CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: mail, news, photos & more. From rjbuesa <@t> yahoo.com Mon Oct 8 13:34:10 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Oct 8 13:34:21 2007 Subject: [Histonet] can I leave IHC sections overnight after first staining? In-Reply-To: <4a722ef70710080937o1e8e2ffp6a2f0d735026edfd@mail.gmail.com> Message-ID: <578619.64960.qm@web61212.mail.yahoo.com> If you are FORCED to leave them over night, leave them in PBS. Ren? J. Moran Elishmereni wrote: Hi Histonetters, Is it possible to leave sections (that were stained with primary Ab) overnight, and counterstain the day after? I am using DAB reaction. Can I leave it dry (or wet, in distilled water) overnight? Thanks, Moran -- Moran Elishmereni Department of Pharmacology and Experimental Therapeutics School of Pharmacy, Faculty of Medicine The Hebrew University of Jerusalem POB 12065 Jerusalem 91120, ISRAEL Tel: 972-2-675-8746 Fax: 972-2-675-8144 Email: moran.elish@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Fussy? Opinionated? Impossible to please? Perfect. Join Yahoo!'s user panel and lay it on us. From RSRICHMOND <@t> aol.com Mon Oct 8 14:50:08 2007 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Mon Oct 8 14:50:19 2007 Subject: [Histonet] Re: prostate triple stain Message-ID: Multiple antibody stains done on prostate biopsy specimens require particularly careful attention to the details of fixation. Jonathan Epstein, the Johns Hopkins pathologist who's the grand guru of prostate pathology (well, one of them, anyway) notes that he has a very high rate of success with in-house biopsy specimens, all of which are fixed in exactly the same way, but has much less satisfactory results with paraffin blocks sent in consultation from other pathology services. If you use such a stain, expect to work on it a bit so that it works in your particular fixation and processing systems. Bob Richmond Samurai Pathologist Knoxville TN From sccrshlly <@t> yahoo.com Mon Oct 8 21:02:15 2007 From: sccrshlly <@t> yahoo.com (Shelly Coker) Date: Mon Oct 8 21:02:33 2007 Subject: [Histonet] Re; Cell block Message-ID: <192730.65341.qm@web90301.mail.mud.yahoo.com> Many times, the clots are simply fibrin, which does not usually yield the cells the pathologist are looking for. If you spin down the fluid, the cells that the pathologist wants to see are found in the button, however small, in the bottom of the tube. We used 50 ml tubes and spun them down. If the specimen is scant, you can always make smears, or use histogel to keep the clot intact. Either way, you obtain far superior results spinning down the unclotted fluid, rather than just using the clot. --------------------------------- Yahoo! oneSearch: Finally, mobile search that gives answers, not web links. From Kemlo.Rogerson <@t> waht.swest.nhs.uk Tue Oct 9 02:03:39 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Oct 9 02:03:56 2007 Subject: [Histonet] Cell block Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222EEA0@wahtntex2.waht.swest.nhs.uk> Submit the clot for processing as it tends to 'suck' cells into itself, but carry out a Cytospin or LBC on the native fluid too. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Creativity requires the courage to let go of certainties. --Erich Fromm This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Kemlo.Rogerson <@t> waht.swest.nhs.uk Tue Oct 9 02:04:55 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Oct 9 02:05:07 2007 Subject: [Histonet] can I leave IHC sections overnight after firststaining? Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222EEA1@wahtntex2.waht.swest.nhs.uk> Why would you do all the staining, then stop something that takes less the 10 more minutes to finish? Just asking. Mike 10 mins is a long time if you are a fly, not so long if you are a tortoise. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Creativity requires the courage to let go of certainties. --Erich Fromm This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Kemlo.Rogerson <@t> waht.swest.nhs.uk Tue Oct 9 02:09:29 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Oct 9 02:09:42 2007 Subject: [Histonet] An idea that needs input--HT Continuing ed game--for free Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222EEA2@wahtntex2.waht.swest.nhs.uk> I LOVE what I do--I'm a histotech and a recruiter--and have read lots of discussion on inequities in education, experience, skills, understanding by histotechs (including me!) who do this work. Some of us are registered, some aren't, some are required to do continuing ed, some aren't. Some have made this a career, for some it's a job. I would like to come up with a format to provide accredited continuing ed that is FUN so it is used, increases our understanding of what we do and why--and lots of other good things. Cheryl Um.... I think this is where American culture and British culture tends to diverge? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Creativity requires the courage to let go of certainties. --Erich Fromm This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From sheila_adey <@t> hotmail.com Tue Oct 9 05:54:22 2007 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Tue Oct 9 05:54:39 2007 Subject: [Histonet] Toe nails... Message-ID: Good morning netters: We occasionally receive toenails for routine processing and each time it is difficult to decide what is the best method for "softening" the nail. Any suggestions would be greatly appreciated. Thnanks in advance and have a great day!! Sheila Adey HT MLTPort Huron HospitalMichigan _________________________________________________________________ Send a smile, make someone laugh, have some fun! Start now! http://www.freemessengeremoticons.ca/?icid=EMENCA122 From jnocito <@t> satx.rr.com Tue Oct 9 06:01:01 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Oct 9 06:01:23 2007 Subject: [Histonet] Toe nails... References: Message-ID: <000701c80a63$aeb1ff40$0302a8c0@yourxhtr8hvc4p> Sheila, Joe the Toe recommends 20% sodium hydroxide for 1 hour before processing. JTT ----- Original Message ----- From: "sheila adey" To: Sent: Tuesday, October 09, 2007 5:54 AM Subject: [Histonet] Toe nails... Good morning netters: We occasionally receive toenails for routine processing and each time it is difficult to decide what is the best method for "softening" the nail. Any suggestions would be greatly appreciated. Thnanks in advance and have a great day!! Sheila Adey HT MLTPort Huron HospitalMichigan _________________________________________________________________ Send a smile, make someone laugh, have some fun! Start now! http://www.freemessengeremoticons.ca/?icid=EMENCA122_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Oct 9 08:05:52 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Oct 9 08:06:06 2007 Subject: [Histonet] Toe nails... In-Reply-To: Message-ID: <545951.10397.qm@web61219.mail.yahoo.com> Sheila: What has worked the best for me is placing the nails in an all plastic cassette, and immmersing it in 10% sodium hydroxide. Check the nail after 30 minutes and decide then to leave them somewhat longer or wash them thoroughly before start the processing. Under separate cover I am sending a review I wrote about toe nails. Ren? J. sheila adey wrote: Good morning netters: We occasionally receive toenails for routine processing and each time it is difficult to decide what is the best method for "softening" the nail. Any suggestions would be greatly appreciated. Thnanks in advance and have a great day!! Sheila Adey HT MLTPort Huron HospitalMichigan _________________________________________________________________ Send a smile, make someone laugh, have some fun! Start now! http://www.freemessengeremoticons.ca/?icid=EMENCA122_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: mail, news, photos & more. From richardsls <@t> upmc.edu Tue Oct 9 08:21:55 2007 From: richardsls <@t> upmc.edu (Richards, Linda (Pathology)) Date: Tue Oct 9 08:22:13 2007 Subject: [Histonet] Open Histology Positions Message-ID: <58085D8E8D09D34497D5595559B1A38B0B7E4CE5@1upmc-msx5.acct.upmchs.net> Hello Everyone, I am a histology supervisor at UPMC Presbyterian Shadyside histology laboratories. I am supervisor of the histology labs at both hospitals. I currently have four open histology positions. We are a high volume, highly specialized lab, with potential to learn and experience many things not found in a smaller hospital setting. We offer the opportunity to learn at least 50 special stains, over 150 immunoperoxidase stains, and enzymehistochemical staining. You can experience the opportunity of cutting whole eyeballs, serial cutting frozen muscle, and teasing nerves as an example of some of our specialized techniques. We offer a $2000.00 sign on bonus, up to $1000.00 assistance with moving expenses, and tuition assistance for education benefits. We have all new, state of the art equipment. We do work weekends and holidays by rotation. We offer a comparable salary. Pittsburgh is a beautiful city, with lots of entertaining things to do. If anyone is interested, please contact me at the phone numbers or email listed below. You can also go to our website at UPMC.com, click on careers, and put in your application online. Thank you for your consideration. Linda Linda S. Richards Supervisor of Histology UPMC Presbyterian Shadyside Phone: Presbyterian - 412-647-7740 Phone: Shadyside - 412-623-2336 Pager: 888.289.2510 Email: richardsls@upmc.edu From gu.lang <@t> gmx.at Tue Oct 9 09:29:32 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Oct 9 09:29:46 2007 Subject: AW: [Histonet] Re: prostate triple stain In-Reply-To: Message-ID: <003201c80a80$cf8e24b0$6412a8c0@dielangs.at> I have heard similar about p504s, that's becomes false positive in underfixed prostata. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Robert Richmond Gesendet: Montag, 08. Oktober 2007 21:50 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Re: prostate triple stain Multiple antibody stains done on prostate biopsy specimens require particularly careful attention to the details of fixation. Jonathan Epstein, the Johns Hopkins pathologist who's the grand guru of prostate pathology (well, one of them, anyway) notes that he has a very high rate of success with in-house biopsy specimens, all of which are fixed in exactly the same way, but has much less satisfactory results with paraffin blocks sent in consultation from other pathology services. If you use such a stain, expect to work on it a bit so that it works in your particular fixation and processing systems. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSTONE <@t> CMHLINK.ORG Tue Oct 9 09:40:08 2007 From: MSTONE <@t> CMHLINK.ORG (MARCELLYN STONE) Date: Tue Oct 9 09:39:02 2007 Subject: [Histonet] CRYOSTAT TROUBLESHOOTING Message-ID: <000901c80a82$52cfb770$fc050180@corp.cmhlink.org> Hello.. I am looking for some help. The O.C.T. embedded tissue is falling of the chuck when we try to section. Does anyone have any ideas to stop this? We have tried new O.C.T., cleaning chucks, advancing slower. Please help. Thanks Marcy From rjbuesa <@t> yahoo.com Tue Oct 9 09:46:18 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Oct 9 09:46:33 2007 Subject: [Histonet] CRYOSTAT TROUBLESHOOTING In-Reply-To: <000901c80a82$52cfb770$fc050180@corp.cmhlink.org> Message-ID: <432289.31340.qm@web61225.mail.yahoo.com> Temperature is the main issue, as well as the use of the "heavy mass" to press the the tissue and complete it freezing. You first have to add the OCT over the cold chuck, then add the tissue, then more OCT and then press everything with the heavy mass (the round piece of metal with the handle in all cryostats). When everything if frozen, cut out the excess OCT and start sectioning. If the temperature is adequate (at least -20?C), the OCT should not come off the chuck. Ren? J. MARCELLYN STONE wrote: Hello.. I am looking for some help. The O.C.T. embedded tissue is falling of the chuck when we try to section. Does anyone have any ideas to stop this? We have tried new O.C.T., cleaning chucks, advancing slower. Please help. Thanks Marcy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Fussy? Opinionated? Impossible to please? Perfect. Join Yahoo!'s user panel and lay it on us. From vazquezr <@t> ohsu.edu Tue Oct 9 10:01:53 2007 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Tue Oct 9 10:02:29 2007 Subject: [Histonet] CRYOSTAT TROUBLESHOOTING Message-ID: Is the chucks cold or warm when you apply the OCT? Sometimes if it is cold, the OCT can't get into the cracks of the chuck to adhede to it...hope that helps. Robyn OHSU >>> "MARCELLYN STONE" 10/9/2007 7:40 AM >>> Hello.. I am looking for some help. The O.C.T. embedded tissue is falling of the chuck when we try to section. Does anyone have any ideas to stop this? We have tried new O.C.T., cleaning chucks, advancing slower. Please help. Thanks Marcy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tthinnes <@t> scripps.edu Tue Oct 9 10:06:14 2007 From: tthinnes <@t> scripps.edu (terri thinnes) Date: Tue Oct 9 10:05:30 2007 Subject: [Histonet] CRYOSTAT TROUBLESHOOTING In-Reply-To: <000901c80a82$52cfb770$fc050180@corp.cmhlink.org> Message-ID: <000201c80a85$ef82bd30$21d48389@innateimmunweb> Dear Marcy. The chuck is really cold. Take your thumb and warm up the chuck before you apply the OCT. This solved my similar problem. Good luck. Terri -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MARCELLYN STONE Sent: Tuesday, October 09, 2007 7:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CRYOSTAT TROUBLESHOOTING Hello.. I am looking for some help. The O.C.T. embedded tissue is falling of the chuck when we try to section. Does anyone have any ideas to stop this? We have tried new O.C.T., cleaning chucks, advancing slower. Please help. Thanks Marcy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lynn.Burton <@t> Illinois.gov Tue Oct 9 10:16:30 2007 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Tue Oct 9 10:18:07 2007 Subject: [Histonet] Toe nails... References: Message-ID: We have used Nair Hair removal solution for several years. Depending upon the thickness of the nail,we leave it in overnight or even for the weekend. It cuts like a hot knife through butter after that. Lynn Burton ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of sheila adey Sent: Tue 10/9/2007 5:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Toe nails... Good morning netters: We occasionally receive toenails for routine processing and each time it is difficult to decide what is the best method for "softening" the nail. Any suggestions would be greatly appreciated. Thnanks in advance and have a great day!! Sheila Adey HT MLTPort Huron HospitalMichigan _________________________________________________________________ Send a smile, make someone laugh, have some fun! Start now! http://www.freemessengeremoticons.ca/?icid=EMENCA122_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Tue Oct 9 10:24:35 2007 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Tue Oct 9 10:24:40 2007 Subject: [Histonet] CRYOSTAT TROUBLESHOOTING In-Reply-To: <000901c80a82$52cfb770$fc050180@corp.cmhlink.org> Message-ID: Marcy, I was always taught to apply the OCT to a RT chuck, put in the cryostat and when you start to see the OCT turn white, i.e. the OCT in the grooves is starting to freeze, add the tissue and weight down with the cold heat extractor. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MARCELLYN STONE Sent: Tuesday, October 09, 2007 9:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CRYOSTAT TROUBLESHOOTING Hello.. I am looking for some help. The O.C.T. embedded tissue is falling of the chuck when we try to section. Does anyone have any ideas to stop this? We have tried new O.C.T., cleaning chucks, advancing slower. Please help. Thanks Marcy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mohs76009 <@t> yahoo.com Tue Oct 9 10:52:28 2007 From: mohs76009 <@t> yahoo.com (Matt Bancroft) Date: Tue Oct 9 10:52:35 2007 Subject: [Histonet] CRYOSTAT TROUBLESHOOTING In-Reply-To: Message-ID: <497152.87326.qm@web63415.mail.re1.yahoo.com> I am a manger of a mohs lab, I use room temp molds to keep the OCT from popping off the chuck. Matt Bancroft HT (ASCP) "Sebree Linda A." wrote: Marcy, I was always taught to apply the OCT to a RT chuck, put in the cryostat and when you start to see the OCT turn white, i.e. the OCT in the grooves is starting to freeze, add the tissue and weight down with the cold heat extractor. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MARCELLYN STONE Sent: Tuesday, October 09, 2007 9:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CRYOSTAT TROUBLESHOOTING Hello.. I am looking for some help. The O.C.T. embedded tissue is falling of the chuck when we try to section. Does anyone have any ideas to stop this? We have tried new O.C.T., cleaning chucks, advancing slower. Please help. Thanks Marcy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Moody friends. Drama queens. Your life? Nope! - their life, your story. Play Sims Stories at Yahoo! Games. From mari.ann.mailhiot <@t> leica-microsystems.com Tue Oct 9 11:47:41 2007 From: mari.ann.mailhiot <@t> leica-microsystems.com (mari.ann.mailhiot@leica-microsystems.com) Date: Tue Oct 9 11:47:49 2007 Subject: [Histonet] CRYOSTAT TROUBLESHOOTING In-Reply-To: <000901c80a82$52cfb770$fc050180@corp.cmhlink.org> Message-ID: Marcellyn There are a couple of things that could be happening. Are your chucks and the specimen at room temp? Sometime when the chuck is colder and specimen is warm the OCTand the specimen will separate from the disc. Sometimes it is better to keep the discs at room temp and freeze the warm disc and specimen all at once.. Are you possibly freezing the specimen ahead of time and placing in a low temp freezer? There are varing opinions but I find for me it is easier to equilibrate the temp of my specimen by placing in a -30 freezer before I start cutting at -20. You can also just place the specimen in the cryostat and wait a few hours before cutting. Are you possbily fixing your specimen or cryo - protecting with sucrose before freezing? All of the above things are a factor in freezing a specimen to a chuck. If you have questions just give me a call. Best Regards Mari Ann Mailhiot BA HT ASCP Application Specialist Leica Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com "MARCELLYN STONE" To Sent by: histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] CRYOSTAT TROUBLESHOOTING 10/09/2007 09:40 AM Please respond to mstone@cmhlink.or g Hello.. I am looking for some help. The O.C.T. embedded tissue is falling of the chuck when we try to section. Does anyone have any ideas to stop this? We have tried new O.C.T., cleaning chucks, advancing slower. Please help. Thanks Marcy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From Janet.Bonner <@t> FLHOSP.ORG Tue Oct 9 13:11:58 2007 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Tue Oct 9 13:12:14 2007 Subject: [Histonet] CRYOSTAT TROUBLESHOOTING References: <497152.87326.qm@web63415.mail.re1.yahoo.com> Message-ID: <5F31F38C96781A4FBE3196EBC22D47802E1162@fhosxchmb006.ADVENTISTCORP.NET> We also use the warm chucks. Without exception, the OCT chunks off the chuck when the chuck is pre-cooled. We have chucks of all ages and stages of use. We only use the large chucks and clean them in a virex soak and a wire brush. Both of these last specifications may be factors! For those of you who precool your chucks, what size/type of chuck are you using and what do you clean them with? The chucks come out so much neater if the chuck is cooler before the OCT is applied! Thank you! Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Matt Bancroft Sent: Tue 10/9/2007 11:52 AM To: Sebree Linda A.; mstone@cmhlink.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CRYOSTAT TROUBLESHOOTING I am a manger of a mohs lab, I use room temp molds to keep the OCT from popping off the chuck. Matt Bancroft HT (ASCP) "Sebree Linda A." wrote: Marcy, I was always taught to apply the OCT to a RT chuck, put in the cryostat and when you start to see the OCT turn white, i.e. the OCT in the grooves is starting to freeze, add the tissue and weight down with the cold heat extractor. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MARCELLYN STONE Sent: Tuesday, October 09, 2007 9:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CRYOSTAT TROUBLESHOOTING Hello.. I am looking for some help. The O.C.T. embedded tissue is falling of the chuck when we try to section. Does anyone have any ideas to stop this? We have tried new O.C.T., cleaning chucks, advancing slower. Please help. Thanks Marcy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Moody friends. Drama queens. Your life? Nope! - their life, your story. Play Sims Stories at Yahoo! Games. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From Malcolm.McCallum <@t> tamut.edu Tue Oct 9 14:25:34 2007 From: Malcolm.McCallum <@t> tamut.edu (Malcolm McCallum) Date: Tue Oct 9 14:26:20 2007 Subject: [Histonet] misinformation outlet poised as journal References: <497152.87326.qm@web63415.mail.re1.yahoo.com> <5F31F38C96781A4FBE3196EBC22D47802E1162@fhosxchmb006.ADVENTISTCORP.NET> Message-ID: Recently, I received an article on "how carbon dioxide does not contribute to climate change." The article is clearly misinformation and was published in a journal called "The Journal of American Physicians and Surgeons (http://www.jpands.org/ )." Most of the article is a fake article that was circulated back in the 1990's. EVERYONE NEEDS TO CHECK THIS OUT! http://www.jpands.org/vol12no3/robinson.pdf They say they do double blind peer review, but who peer reviewed it? Are any of these folks atmospheric scientists, or for that matter even evironmetally aware? Furthermore, looking right at figure 2 you can see how biased their deductions are. Examining the figure you see that they draw a vertical line suggesting that hydrocarbons were not in use prior to that line. What the heck is coal then? Last time I checked coal is a hydrocarbon compound! Apparently this is not limited to climate issues as published in this blog (http://autismnaturalvariation.blogspot.com/2006/03/open-letter-to-journal-of-physicians.html ) on Autism where they request an article be retracted: "downward trends in neurodevelopmental disorders following removal of thimersoal-containing vaccines" Spring 2006. Main criticisms: 1. methodological flaws 2. factual errors 3. misleading use of terminology 4. the article should be retracted Notice the Wikipedia discussion regarding this journal: http://en.wikipedia.org/wiki/Association_of_American_Physicians_and_Surgeons Articles published in the journal have argued that the Food and Drug Administration and Centers for Medicare and Medicaid Services are unconstitutional,[27] that "humanists" have conspired to replace the "creation religion of Jehovah" with evolution, [28] that increased carbon dioxide in the atmosphere has not caused global warming, [29] that HIV does not cause AIDS,[30] and that the "gay male lifestyle" shortens life expectancy by 20 years.[31] A series of articles by pro-life authors also claimed a link between abortion and breast cancer;[32][33] such a link has been rejected by the National Cancer Institute.[34] The journal is not listed in the major literature databases of MEDLINE/PubMed[35] nor the Web of Science.[36] Quackwatch lists JPandS as an untrustworthy, non-recommended periodical.[37] The World Health Organization found that a 2003 article on vaccination published in the journal had "a number of limitations which undermine the conclusions drawn by the authors", although it noted that the matters raised in the paper were of sufficient importance that "WHO and GACVS will continue to keep the issue under careful and ongoing review."[38] Investigative journalist Brian Deer wrote that the journal is the "house magazine of a right-wing American fringe group [AAPS]" and "is barely credible as an independent forum."[39]" I recommend that people inform the Directory of Open Access Journals about this journal/article as they are trying to be reputable, but clearly this journal is not. Also, we need to publicize that this journal is little more than political tribe. Malcolm L. McCallum Assistant Professor Department of Biological Sciences Texas A&M University Texarkana 2600 Robison Rd. Texarkana, TX 75501 O: 1-903-223-3134 H: 1-903-791-3843 Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html VISIT HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org A New Journal Published in Partnership with Partners in Amphibian and Reptile Conservation and the World Congress of Herpetology. Fall Teaching Schedule & Office Hours: Mammalogy: MW 2-4 pm Cell Biology: TR 10-11:40 am Ecology: W 6-10 pm Offic Hours: MW 1-2, TR 11:40-12:40 "We live in a time when lemonade is made with artificial flavoring, and furnisher polish is made with fresh lemons." -Alfred E. Neuman From godsgalnow <@t> aol.com Tue Oct 9 14:57:38 2007 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Tue Oct 9 14:58:14 2007 Subject: [Histonet] pten Message-ID: <8C9D8C5C77125F8-F00-6A51@Webmail-mg16.sysops.aol.com> This is a relatively new antibody, is anyone out there using it? ?have been playing with it would like to talk with others...for suggestions and tips. Roxanne ________________________________________________________________________ Email and AIM finally together. You've gotta check out free AOL Mail! - http://mail.aol.com From jmbruno <@t> email.unc.edu Tue Oct 9 15:00:46 2007 From: jmbruno <@t> email.unc.edu (jmbruno) Date: Tue Oct 9 15:01:14 2007 Subject: [Histonet] Matrigel sectioning. Message-ID: I have formalin fixed matrigel plugs imbedded in OCT that I am attempting to section with a cryostat. The matrigel "crumples" when cut and I end up with a large hole in that space. Obviously I am seeking advice on how to avoid this problem. Thanks, Jon From anitathorn <@t> comcast.net Tue Oct 9 15:11:40 2007 From: anitathorn <@t> comcast.net (anitathorn@comcast.net) Date: Tue Oct 9 15:11:45 2007 Subject: [Histonet] CRYOSTAT TROUBLESHOOTING Message-ID: <100920072011.29943.470BE07C000BD152000074F72200750330029D01089B0E9B07020E@comcast.net> That's exactly how I do it too. The trick is pulling the chuck off of the freeze bar when there is still a liquid center and then placing the tissue in this "puddle". I then add a little more OCT on top, put the chuck back onto the freeze bar and weigh this down with the heat extractor. If I don't catch it before it freezes all the way, I simple score the OCT with a scalpel blade to help create a bond with the subsequently added RT tissue. -------------- Original message ---------------------- From: "Sebree Linda A." > Marcy, > > I was always taught to apply the OCT to a RT chuck, put in the cryostat > and when you start to see the OCT turn white, i.e. the OCT in the > grooves is starting to freeze, add the tissue and weight down with the > cold heat extractor. > > Linda Sebree, HT(ASCP) > University of Wisconsin Hospital & Clinics > IHC/ISH Laboratory > A4/204-3224 > 600 Highland Ave. > Madison, WI 53792 > (608)265-6596 > FAX: (608)262-7174 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > MARCELLYN STONE > Sent: Tuesday, October 09, 2007 9:40 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] CRYOSTAT TROUBLESHOOTING > > > Hello.. > > I am looking for some help. The O.C.T. embedded tissue is falling of > the > chuck when we try to section. Does anyone have any ideas to stop this? > We > have tried new O.C.T., cleaning chucks, advancing slower. Please help. > Thanks Marcy > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mariakatleba <@t> aol.com Tue Oct 9 15:18:15 2007 From: mariakatleba <@t> aol.com (mariakatleba@aol.com) Date: Tue Oct 9 15:18:26 2007 Subject: [Histonet] non pathologist grossing procedure Message-ID: <8C9D8C8A852EED4-D6C-6CE5@mblk-d10.sysops.aol.com> Does anyone have a "non pathologist grossing procedure" I could use as a template? I am working on my P&P. Regards, Maria Katleba MS HT(ASCP) Queen of the Valley Medical Center Pathology Dept Manager Napa CA 94558 707-252-4411 x2563 ________________________________________________________________________ Email and AIM finally together. You've gotta check out free AOL Mail! - http://mail.aol.com From NMargaryan <@t> childrensmemorial.org Tue Oct 9 15:27:41 2007 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Tue Oct 9 15:25:49 2007 Subject: [Histonet] I need help with ISH Message-ID: Dear Histonetters: I see you are from IHC/ISH Lab and I am wondering if you could help me with the ISH problems, I am having. I am getting staining/signals in my negative controls, in negative probes, no probes, no Primary Abs. These are my steps. I could not get where mistake is. I plated cells on the coverslip glass. Wait they grow for 2-3 days. Washed RPMI with PBS, fixed in 2 different ways: 1. 4%paraformyldehyde and store in 70% ethanol or used right away, 2. air dry cells for an hour and fix in cold methanol for 10sec follow by cold acetone 3x5sec. Then using HybriProbe from Biognostik followed their protocol made PREHYBRIDIZATION steps: 1. rehydrate to 70% ethanol, 2. incubate with HybriBuffer for 3-4 h at 30C 3. hybridize o/n at 30C 4. quick rinse 2x in 1xSSC then for 5min 5. quick rinse 2x in 0.1xSSC then for 15min at 40C and 45C 6. quick wash/not wash with H2O then using kit from DAKO (CSA II, biotin free, tyramide signal, Amplification System 7. H2O2 block for 5 and 10 min then water wash and wash with TRIS Buffer with tween 8. Protein Block 5 and 8 min 9. Ab primary for 15 min and 3x5 min wash 10. Secondary Rb link(K1501) for 15 min and 3x5 min wash 11. Amplification reagent for 15 min and 3x5 min wash 12. Anti-fluorescein-HRP for 15 min and 3x5 min wash 13. DAB and Counterstainig. Please, let me know what you think. Sincerely, Naira Naira V. Margaryan, D.V.M., Ph.D. Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3394 Tel: 773-755-6340 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 From sonyprasad <@t> hotmail.com Tue Oct 9 16:05:26 2007 From: sonyprasad <@t> hotmail.com (sony prasad) Date: Tue Oct 9 16:05:31 2007 Subject: [Histonet] SENESCENCE MARKERS Message-ID: Hi, I am looking at using some senescence markers for cultured cells as well as FFPE tissue sections. Has anyone used Senescence associated B-Gal staining. It would be a great help if I could get a protocol for carrying it out and also the company where this marker can be obtained. I am fairly new to these stainings so would highly appreciate if I could possibly get a detailed protocol. Please also advice of any other markers and protocols that have worked well for you. Many thanks, Sony _________________________________________________________________ Check out some new online services at Windows Live Ideas?so new they haven?t even been officially released yet. http://www.msnspecials.in/windowslive/ From sccrshlly <@t> yahoo.com Tue Oct 9 18:59:09 2007 From: sccrshlly <@t> yahoo.com (Shelly Coker) Date: Tue Oct 9 18:59:13 2007 Subject: [Histonet] Toe nails... Message-ID: <325350.69411.qm@web90309.mail.mud.yahoo.com> At my previous job, we used 10% hydrogen peroxide to soften nails. You simply trim into the nail, then soak the nail. Once softened, we could cut beautiful sections of nail. If the pieces are just too big to even trim, soak the pieces individually in peroxide, then embed and cut them. This was a trick that was brought to us by a tech from Las Vegas! --------------------------------- Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: mail, news, photos & more. From tissuearray <@t> hotmail.com Tue Oct 9 21:55:09 2007 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Tue Oct 9 21:55:16 2007 Subject: [Histonet] Toe nails... In-Reply-To: <325350.69411.qm@web90309.mail.mud.yahoo.com> References: <325350.69411.qm@web90309.mail.mud.yahoo.com> Message-ID: I have cut toe nail and even horse hoof using 10% ammonia water. Soak the specimen in the 10% ammonia water until it's flexible and then process it. One processed, trim in ever-so-slightly not to chunk it and soak again if needed. works wonderful. You might want to add glue to the slide so the nail stays on during staining. Thom arrayworkshop.com arraymold.com > Date: Tue, 9 Oct 2007 16:59:09 -0700> From: sccrshlly@yahoo.com> To: histonet@lists.utsouthwestern.edu> Subject: [Histonet] Toe nails...> > > At my previous job, we used 10% hydrogen peroxide to soften nails. You simply trim into the nail, then soak the nail. Once softened, we could cut beautiful sections of nail. If the pieces are just too big to even trim, soak the pieces individually in peroxide, then embed and cut them. This was a trick that was brought to us by a tech from Las Vegas!> > > ---------------------------------> Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: mail, news, photos & more. > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Peek-a-boo FREE Tricks & Treats for You! http://www.reallivemoms.com?ocid=TXT_TAGHM&loc=us From mickie25 <@t> netzero.net Wed Oct 10 06:45:40 2007 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Wed Oct 10 06:45:54 2007 Subject: [Histonet] CRYOSTAT TROUBLESHOOTING In-Reply-To: <000901c80a82$52cfb770$fc050180@corp.cmhlink.org> References: <000901c80a82$52cfb770$fc050180@corp.cmhlink.org> Message-ID: Dear Marcy, I concur, The primary cause of OCT coming loose from the chuck is the chuck being pre-cooled in the cryostat. The OCT freezes before it can flow to the bottom of the grooves and thus it will not hold. Always start with a room temp chuck and make build-ups one at a time. Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MARCELLYN STONE Sent: Tuesday, October 09, 2007 7:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CRYOSTAT TROUBLESHOOTING Hello.. I am looking for some help. The O.C.T. embedded tissue is falling of the chuck when we try to section. Does anyone have any ideas to stop this? We have tried new O.C.T., cleaning chucks, advancing slower. Please help. Thanks Marcy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From settembr <@t> umdnj.edu Wed Oct 10 08:07:13 2007 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Wed Oct 10 08:08:06 2007 Subject: [Histonet] pten Message-ID: Hello Roxanne, I use PTEN made in mouse from NovoCastra (distributed by VisionBioSystems in Norwell, MA) It is an IVD. I use tonsil as a control and I pretreat with Dako's Target Retreival Solution in a steamer for 40 minutes I use it at 1:75. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> 10/09/07 3:57 PM >>> This is a relatively new antibody, is anyone out there using it? ?have been playing with it would like to talk with others...for suggestions and tips. Roxanne ________________________________________________________________________ Email and AIM finally together. You've gotta check out free AOL Mail! - http://mail.aol.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wia2005 <@t> med.cornell.edu Wed Oct 10 08:24:42 2007 From: wia2005 <@t> med.cornell.edu (William Ares) Date: Wed Oct 10 08:24:51 2007 Subject: [Histonet] H&E Staining Message-ID: i'm currently trying some H&E staining (post-TUNEL) on paraffin embedded tissues and keep having the eosin wash out during the dehydration steps (stepwise: 70% alcohol, 95% alcohol, 100% alcohol)... any ideas on why this would happen? am i missing a step in between that allows the stain to 'stick'? thanks William Ares Research Technician Laboratory of Molecular and Developmental Neuroscience Weill Medical College of Cornell University Tel: (212) 746 5056 Email: wia2005@med.cornell.edu From mpence <@t> grhs.net Wed Oct 10 08:32:04 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Oct 10 08:32:14 2007 Subject: [Histonet] Toe nails... In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C754@IS-E2K3.grhs.net> Isn't that an oxymoron to add glue to a slide that you are going to put horse hoofs on! Is it Friday yet?? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thom Jensen Sent: Tuesday, October 09, 2007 9:55 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Toe nails... I have cut toe nail and even horse hoof using 10% ammonia water. Soak the specimen in the 10% ammonia water until it's flexible and then process it. One processed, trim in ever-so-slightly not to chunk it and soak again if needed. works wonderful. You might want to add glue to the slide so the nail stays on during staining. Thom arrayworkshop.com arraymold.com > Date: Tue, 9 Oct 2007 16:59:09 -0700> From: sccrshlly@yahoo.com> To: > histonet@lists.utsouthwestern.edu> Subject: [Histonet] Toe nails...> > > At my previous job, we used 10% hydrogen peroxide to soften nails. You simply trim into the nail, then soak the nail. Once softened, we could cut beautiful sections of nail. If the pieces are just too big to even trim, soak the pieces individually in peroxide, then embed and cut them. This was a trick that was brought to us by a tech from Las Vegas!> > > ---------------------------------> Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: mail, news, photos & more. > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Peek-a-boo FREE Tricks & Treats for You! http://www.reallivemoms.com?ocid=TXT_TAGHM&loc=us_______________________ ________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Wed Oct 10 08:39:32 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Wed Oct 10 08:39:38 2007 Subject: [Histonet] Toe nails... In-Reply-To: <661949901A768E4F9CC16D8AF8F2838CA1C754@IS-E2K3.grhs.net> References: <661949901A768E4F9CC16D8AF8F2838CA1C754@IS-E2K3.grhs.net> Message-ID: Same difference!. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: 10 October 2007 14:32 To: Thom Jensen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Toe nails... Isn't that an oxymoron to add glue to a slide that you are going to put horse hoofs on! Is it Friday yet?? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thom Jensen Sent: Tuesday, October 09, 2007 9:55 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Toe nails... I have cut toe nail and even horse hoof using 10% ammonia water. Soak the specimen in the 10% ammonia water until it's flexible and then process it. One processed, trim in ever-so-slightly not to chunk it and soak again if needed. works wonderful. You might want to add glue to the slide so the nail stays on during staining. Thom arrayworkshop.com arraymold.com > Date: Tue, 9 Oct 2007 16:59:09 -0700> From: sccrshlly@yahoo.com> To: > histonet@lists.utsouthwestern.edu> Subject: [Histonet] Toe nails...> > > At my previous job, we used 10% hydrogen peroxide to soften nails. You simply trim into the nail, then soak the nail. Once softened, we could cut beautiful sections of nail. If the pieces are just too big to even trim, soak the pieces individually in peroxide, then embed and cut them. This was a trick that was brought to us by a tech from Las Vegas!> > > ---------------------------------> Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: mail, news, photos & more. > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Peek-a-boo FREE Tricks & Treats for You! http://www.reallivemoms.com?ocid=TXT_TAGHM&loc=us_______________________ ________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Wed Oct 10 08:40:30 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Oct 10 08:40:37 2007 Subject: [Histonet] H&E Staining Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222EEEA@wahtntex2.waht.swest.nhs.uk> i'm currently trying some H&E staining (post-TUNEL) on paraffin embedded tissues and keep having the eosin wash out during the dehydration steps (stepwise: 70% alcohol, 95% alcohol, 100% alcohol)... any ideas on why this would happen? am i missing a step in between that allows the stain to 'stick'? thanks It is 'stuck' but you are unsticking it. Either because you are using eosin that is soluble in alcohol or because the water in the 70% is removing the water soluble eosin. You could try adding calcium ions as an accentuator, that might make the glue stronger, but of course you must use aqueous eosin or the calcium ions come out of solution. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Creativity requires the courage to let go of certainties. --Erich Fromm This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Farnana <@t> nehealth.com Wed Oct 10 08:52:38 2007 From: Farnana <@t> nehealth.com (Amy Farnan) Date: Wed Oct 10 08:52:59 2007 Subject: [Histonet] oct falling off the chuck Message-ID: <470CA0E5.26ED.00D9.0@nehealth.com> We find that it works best to warm the cold chuck slightly with your palm before adding OCT. If the chuck is to cold it will not adhere resulting in popping off the chuck. When you add OCT if it instantly freezes when added warm chuck more before adding. It should go on with out starting to freeze immediately. This will not delay the rapid freeze process once it is put on your cold plate. It will still freeze rapidly for you and will not pop off. Disclaimer: The information in this message is confidential. If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender. From gu.lang <@t> gmx.at Wed Oct 10 09:43:15 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Oct 10 09:43:33 2007 Subject: AW: [Histonet] I need help with ISH In-Reply-To: Message-ID: <002001c80b4b$e4749f00$6412a8c0@dielangs.at> Naira, I may be too stupid, but I don't understand your procedure. First I don't see the denaturation-step. What is the antigen to the primary-AB? Is the secondary AB marked with Fluorescein? I thought the CSA-System needs HRP on the site of the antigen and let biotinylated Thyramid precipitate in the surrounding. What is the content of the "Amplification reagens"? What is your testing for? Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Margaryan, Naira Gesendet: Dienstag, 09. Oktober 2007 22:28 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] I need help with ISH Dear Histonetters: I see you are from IHC/ISH Lab and I am wondering if you could help me with the ISH problems, I am having. I am getting staining/signals in my negative controls, in negative probes, no probes, no Primary Abs. These are my steps. I could not get where mistake is. I plated cells on the coverslip glass. Wait they grow for 2-3 days. Washed RPMI with PBS, fixed in 2 different ways: 1. 4%paraformyldehyde and store in 70% ethanol or used right away, 2. air dry cells for an hour and fix in cold methanol for 10sec follow by cold acetone 3x5sec. Then using HybriProbe from Biognostik followed their protocol made PREHYBRIDIZATION steps: 1. rehydrate to 70% ethanol, 2. incubate with HybriBuffer for 3-4 h at 30C 3. hybridize o/n at 30C 4. quick rinse 2x in 1xSSC then for 5min 5. quick rinse 2x in 0.1xSSC then for 15min at 40C and 45C 6. quick wash/not wash with H2O then using kit from DAKO (CSA II, biotin free, tyramide signal, Amplification System 7. H2O2 block for 5 and 10 min then water wash and wash with TRIS Buffer with tween 8. Protein Block 5 and 8 min 9. Ab primary for 15 min and 3x5 min wash 10. Secondary Rb link(K1501) for 15 min and 3x5 min wash 11. Amplification reagent for 15 min and 3x5 min wash 12. Anti-fluorescein-HRP for 15 min and 3x5 min wash 13. DAB and Counterstainig. Please, let me know what you think. Sincerely, Naira Naira V. Margaryan, D.V.M., Ph.D. Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3394 Tel: 773-755-6340 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Wed Oct 10 09:46:52 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Oct 10 09:47:09 2007 Subject: AW: [Histonet] H&E Staining In-Reply-To: Message-ID: <002101c80b4c$6606f090$6412a8c0@dielangs.at> Make the washing steps very short and begin with 96% alcohol, if you use alcoholic eosin. (15-30 sek. 96%-100%-100%). Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von William Ares Gesendet: Mittwoch, 10. Oktober 2007 15:25 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] H&E Staining i'm currently trying some H&E staining (post-TUNEL) on paraffin embedded tissues and keep having the eosin wash out during the dehydration steps (stepwise: 70% alcohol, 95% alcohol, 100% alcohol)... any ideas on why this would happen? am i missing a step in between that allows the stain to 'stick'? thanks William Ares Research Technician Laboratory of Molecular and Developmental Neuroscience Weill Medical College of Cornell University Tel: (212) 746 5056 Email: wia2005@med.cornell.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aureias <@t> gmail.com Wed Oct 10 09:48:27 2007 From: aureias <@t> gmail.com (Lawrence Own) Date: Wed Oct 10 09:48:38 2007 Subject: [Histonet] Fetal Tissue - In Situ - Fixation Issues Message-ID: <223db0850710100748v490f8459v414fbe69deb3165b@mail.gmail.com> Hi all, I'm a grad student at the University of Michigan, and we're currently doing in situ hybridizations. However, we've been having issues during the fixation process of our mouse fetal brain tissue. For some reason, the brain tissue is falling off or dissolving during the fixation process, while leaving the rest of the tissue mounted (spinal cord, etc.) We're using superfrost slides and using formalin during the fixation. Is it the pH, the slides, the ionic strength that is causing this issue? Or has anyone encountered this issue and found a way around it? We've heard there are better slides for better results, perhaps an alternative fixation process, but at this point, we're open to suggestions. Thank you for your time! -- Lawrence S. Own ********************* University of Michigan, Neuroscience From godsgalnow <@t> aol.com Wed Oct 10 10:12:37 2007 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Wed Oct 10 10:12:58 2007 Subject: Fwd: [Histonet] H&E Staining In-Reply-To: <8C9D966E459CC2B-F94-34E@FWM-M37.sysops.aol.com> References: <86ADE4EB583CE64799A9924684A0FBBF0222EEEA@wahtntex2.waht.swest.nhs.uk> <8C9D966E459CC2B-F94-34E@FWM-M37.sysops.aol.com> Message-ID: <8C9D9672072D998-F94-36D@FWM-M37.sysops.aol.com> -----Original Message----- From: godsgalnow@aol.com To: Kemlo.Rogerson@waht.swest.nhs.uk Sent: Wed, 10 Oct 2007 11:10 am Subject: Re: [Histonet] H&E Staining How long are you in the post-eosin staining?? I would skip the 70 % and go straight to 1 change of 95% and 3 changes of 100%. Roxanne -----Original Message----- From: Kemlo Rogerson To: William Ares ; histonet@lists.utsouthwestern.edu Sent: Wed, 10 Oct 2007 9:40 am Subject: RE: [Histonet] H&E Staining i'm currently trying some H&E staining (post-TUNEL) on paraffin embedded tissues and keep having the eosin wash out during the dehydration steps (stepwise: 70% alcohol, 95% alcohol, 100% alcohol)... any ideas on why this would happen? am i missing a step in between that allows the stain to 'stick'? thanks It is 'stuck' but you are unsticking it. Either because you are using eosin that is soluble in alcohol or because the water in the 70% is removing the water soluble eosin. You could try adding calcium ions as an accentuator, that might make the glue stronger, but of course you must use aqueous eosin or the calcium ions come out of solution. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Creativity requires the courage to let go of certainties. --Erich Fromm This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Email and AIM finally together. You've gotta check out free AOL Mail! ________________________________________________________________________ Email and AIM finally together. You've gotta check out free AOL Mail! - http://mail.aol.com From mcauliff <@t> umdnj.edu Wed Oct 10 10:23:04 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Oct 10 10:23:33 2007 Subject: [Histonet] H&E Staining In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBF0222EEEA@wahtntex2.waht.swest.nhs.uk> References: <86ADE4EB583CE64799A9924684A0FBBF0222EEEA@wahtntex2.waht.swest.nhs.uk> Message-ID: <470CEE58.6050704@umdnj.edu> Put a few drops of glacial acetic acid in the eosin. Without acidification, the stain will not 'adhere' to tissue components. Geoff Kemlo Rogerson wrote: > i'm currently trying some H&E staining (post-TUNEL) on paraffin embedded > tissues and keep having the eosin wash out during the dehydration steps > (stepwise: 70% alcohol, 95% alcohol, 100% alcohol)... any ideas on why > this would happen? am i missing a step in between that allows the stain > to 'stick'? thanks > > It is 'stuck' but you are unsticking it. Either because you are using > eosin that is soluble in alcohol or because the water in the 70% is > removing the water soluble eosin. You could try adding calcium ions as > an accentuator, that might make the glue stronger, but of course you > must use aqueous eosin or the calcium ions come out of solution. > > Kemlo Rogerson > Pathology Manager > DD 01934 647057 or extension 3311 > Mob 07749 754194; Pager 07659 597107; > > Creativity requires the courage to let go of certainties. --Erich Fromm > > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. > Please inform me that this message has gone astray before deleting it. > Thank you for your co-operation > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From schaundrawalton <@t> yahoo.com Wed Oct 10 11:03:51 2007 From: schaundrawalton <@t> yahoo.com (Schaundra Walton) Date: Wed Oct 10 11:03:57 2007 Subject: [Histonet] Iron Staining of Bone Marrows Message-ID: <970248.21844.qm@web58911.mail.re1.yahoo.com> We use a Vetana NEXUS special stainer and stain our bone marrow aspirates and smears with Ventana's iron kit. One of our pathologists has complained (repeatedly) that our irons aren't staining like he expects. Has anyone else had trouble with Ventana's iron kit? Is anyone using a different protocol? Any advise would be greatly appreciated. Schaundra Walton BS HTL(ASCP) Swedish American Hospital 1401 E. State St. Rockford, IL 61104 --------------------------------- Pinpoint customers who are looking for what you sell. From mpence <@t> grhs.net Wed Oct 10 11:14:43 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Oct 10 11:14:58 2007 Subject: [Histonet] Iron Staining of Bone Marrows In-Reply-To: <970248.21844.qm@web58911.mail.re1.yahoo.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C756@IS-E2K3.grhs.net> There is only 3 protocols for the iron stain available. Stain all three and ask him which one he can live with. Maybe you should ask him what he expects then to look like. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Schaundra Walton Sent: Wednesday, October 10, 2007 11:04 AM To: Histonet Subject: [Histonet] Iron Staining of Bone Marrows We use a Vetana NEXUS special stainer and stain our bone marrow aspirates and smears with Ventana's iron kit. One of our pathologists has complained (repeatedly) that our irons aren't staining like he expects. Has anyone else had trouble with Ventana's iron kit? Is anyone using a different protocol? Any advise would be greatly appreciated. Schaundra Walton BS HTL(ASCP) Swedish American Hospital 1401 E. State St. Rockford, IL 61104 --------------------------------- Pinpoint customers who are looking for what you sell. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Wed Oct 10 11:26:49 2007 From: tkngflght <@t> yahoo.com (Cheryl R. Kerry) Date: Wed Oct 10 11:26:47 2007 Subject: [Histonet] Iron Staining of Bone Marrows In-Reply-To: <970248.21844.qm@web58911.mail.re1.yahoo.com> Message-ID: <004801c80b5a$5c520360$6a01a8c0@CHERYLSLAPTOP> Hey Schaundra- Have you talked with Ventana tech support? They've probably dealt with similar issues from other clients and would know right off the bat how to help if there is help to be had. If there isn't there always the old-fashioned alternative to fix this for your Path... I know this isn't what you want to hear, but sometimes automation cannot duplicate what we can produce by hand. If you try what they suggest and he's not getting what he needs to adequately diagnose the patient--he needs to serve the patient's best interest (an accurate diagnosis). You may need to pull these off the machine. Running them by hand isn't all that hard OR time-consuming if your reagents are at hand, and you do end up with a better product. My two cents having been in your shoes in the past... Cheryl Cheryl R. Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab, one great tech at a time. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Schaundra Walton Sent: Wednesday, October 10, 2007 11:04 AM To: Histonet Subject: [Histonet] Iron Staining of Bone Marrows We use a Vetana NEXUS special stainer and stain our bone marrow aspirates and smears with Ventana's iron kit. One of our pathologists has complained (repeatedly) that our irons aren't staining like he expects. Has anyone else had trouble with Ventana's iron kit? Is anyone using a different protocol? Any advise would be greatly appreciated. Schaundra Walton BS HTL(ASCP) Swedish American Hospital 1401 E. State St. Rockford, IL 61104 --------------------------------- Pinpoint customers who are looking for what you sell. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sheila_adey <@t> hotmail.com Wed Oct 10 11:29:27 2007 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Wed Oct 10 11:29:34 2007 Subject: [Histonet] Toe nail results... Message-ID: A BIG thank you to everyone for their great ideas with the toe nails. I tried the 5% NaOH procedure from Rene and the results were so much better that we are going to make that procedure a part of our manuel. Excellent job on the paper Rene. Sheila Adey HT MLTPort Huron HospitalMichigan _________________________________________________________________ Send a smile, make someone laugh, have some fun! Start now! http://www.freemessengeremoticons.ca/?icid=EMENCA122 From TJJ <@t> Stowers-Institute.org Wed Oct 10 12:05:59 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Wed Oct 10 12:06:31 2007 Subject: [Histonet] Re: Matrigel sectioning Message-ID: Jon, I just did a search of the histonet archives yesterday on this very subject. Go to www.histosearch.com/histonet and type in matrigel in the search box. Others have had difficulty with cryosectioning it in the past as well. Apparently it paraffin processes and sections just fine. You might see if this is an option instead. Good luck! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From RSRICHMOND <@t> aol.com Wed Oct 10 12:45:50 2007 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Wed Oct 10 12:45:55 2007 Subject: [Histonet] Re: Toe nail results... Message-ID: Obviously these procedures on hard keratin (toenails) aren't decalcifications, but is anybody billing them with the CPT code 88311 for decalcification (since there is no other applicable code, and there isn't going to be)? If so, how are you describing the procedure in the gross description of the specimen? I'd be inclined to say something like "the specimen is softened in 10% sodium hydroxide (similar to decalcification) before processing". Bob Richmond Samurai Pathologist Knoxville TN From doug <@t> ppspath.com Wed Oct 10 13:52:45 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Wed Oct 10 12:53:04 2007 Subject: {SPAM?} [Histonet] Iron Staining of Bone Marrows In-Reply-To: <970248.21844.qm@web58911.mail.re1.yahoo.com> Message-ID: We are using the NexEs for the iron. No problems what so ever. What is he looking for or missing with them? Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Schaundra Walton Sent: Wednesday, October 10, 2007 11:04 AM To: Histonet Subject: {SPAM?} [Histonet] Iron Staining of Bone Marrows We use a Vetana NEXUS special stainer and stain our bone marrow aspirates and smears with Ventana's iron kit. One of our pathologists has complained (repeatedly) that our irons aren't staining like he expects. Has anyone else had trouble with Ventana's iron kit? Is anyone using a different protocol? Any advise would be greatly appreciated. Schaundra Walton BS HTL(ASCP) Swedish American Hospital 1401 E. State St. Rockford, IL 61104 --------------------------------- Pinpoint customers who are looking for what you sell. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Wed Oct 10 12:54:27 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Oct 10 12:54:34 2007 Subject: AW: [Histonet] Iron Staining of Bone Marrows In-Reply-To: <970248.21844.qm@web58911.mail.re1.yahoo.com> Message-ID: <000601c80b66$9a78b1f0$6412a8c0@dielangs.at> We experienced two times, that the tube of the lid of the container was blocked. I don't know the cause. We changed the pipettes and the staining was well again. You can test it, when you try to get some fluid out of the container with a pipette through the lid. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Schaundra Walton Gesendet: Mittwoch, 10. Oktober 2007 18:04 An: Histonet Betreff: [Histonet] Iron Staining of Bone Marrows We use a Vetana NEXUS special stainer and stain our bone marrow aspirates and smears with Ventana's iron kit. One of our pathologists has complained (repeatedly) that our irons aren't staining like he expects. Has anyone else had trouble with Ventana's iron kit? Is anyone using a different protocol? Any advise would be greatly appreciated. Schaundra Walton BS HTL(ASCP) Swedish American Hospital 1401 E. State St. Rockford, IL 61104 --------------------------------- Pinpoint customers who are looking for what you sell. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Wed Oct 10 13:25:52 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Oct 10 13:25:33 2007 Subject: [Histonet] Iron Staining of Bone Marrows In-Reply-To: <970248.21844.qm@web58911.mail.re1.yahoo.com> Message-ID: Run your control slides in parallel, one set on the instrument and one off using a manual method. Compare the results. This would rule out/in a kit/instrument problem. Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu Schaundra Walton Sent by: histonet-bounces@lists.utsouthwestern.edu 10/10/2007 09:03 AM To Histonet cc Subject [Histonet] Iron Staining of Bone Marrows We use a Vetana NEXUS special stainer and stain our bone marrow aspirates and smears with Ventana's iron kit. One of our pathologists has complained (repeatedly) that our irons aren't staining like he expects. Has anyone else had trouble with Ventana's iron kit? Is anyone using a different protocol? Any advise would be greatly appreciated. Schaundra Walton BS HTL(ASCP) Swedish American Hospital 1401 E. State St. Rockford, IL 61104 --------------------------------- Pinpoint customers who are looking for what you sell. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Wed Oct 10 13:28:10 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Oct 10 13:27:52 2007 Subject: [Histonet] H&E Staining In-Reply-To: <470CEE58.6050704@umdnj.edu> Message-ID: Also, the more water that is in the dehydrating alcohols, the faster the slides will differentiate. You may be washing out the eosin. The optimal pH is 4.6 to 5. Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu Geoff McAuliffe Sent by: histonet-bounces@lists.utsouthwestern.edu 10/10/2007 08:23 AM To Kemlo Rogerson cc William Ares , histonet@lists.utsouthwestern.edu Subject Re: [Histonet] H&E Staining Put a few drops of glacial acetic acid in the eosin. Without acidification, the stain will not 'adhere' to tissue components. Geoff Kemlo Rogerson wrote: > i'm currently trying some H&E staining (post-TUNEL) on paraffin embedded > tissues and keep having the eosin wash out during the dehydration steps > (stepwise: 70% alcohol, 95% alcohol, 100% alcohol)... any ideas on why > this would happen? am i missing a step in between that allows the stain > to 'stick'? thanks > > It is 'stuck' but you are unsticking it. Either because you are using > eosin that is soluble in alcohol or because the water in the 70% is > removing the water soluble eosin. You could try adding calcium ions as > an accentuator, that might make the glue stronger, but of course you > must use aqueous eosin or the calcium ions come out of solution. > > Kemlo Rogerson > Pathology Manager > DD 01934 647057 or extension 3311 > Mob 07749 754194; Pager 07659 597107; > > Creativity requires the courage to let go of certainties. --Erich Fromm > > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. > Please inform me that this message has gone astray before deleting it. > Thank you for your co-operation > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtarango <@t> nvcancer.org Wed Oct 10 13:36:41 2007 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Wed Oct 10 13:36:58 2007 Subject: [Histonet] Iron Staining of Bone Marrows In-Reply-To: <970248.21844.qm@web58911.mail.re1.yahoo.com> Message-ID: <5AEC610C1CE02945BD63A395BA763EDE011B6EA7@NVCIEXCH02.NVCI.org> Is the problem only on the smears? If it is maybe its got a bunch of fatty stuff on top the smears and runs off the slide when you stain on the machine. Why automate Prussian blue anyway? I'm guessing it costs 10x as much too. Mark Adam Tarango HT(ASCP) Histology & IHC Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 Direct Line (702) 822-5112 Mobile (702) 759-9229 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Schaundra Walton Sent: Wednesday, October 10, 2007 9:04 AM To: Histonet Subject: [Histonet] Iron Staining of Bone Marrows We use a Vetana NEXUS special stainer and stain our bone marrow aspirates and smears with Ventana's iron kit. One of our pathologists has complained (repeatedly) that our irons aren't staining like he expects. Has anyone else had trouble with Ventana's iron kit? Is anyone using a different protocol? Any advise would be greatly appreciated. Schaundra Walton BS HTL(ASCP) Swedish American Hospital 1401 E. State St. Rockford, IL 61104 --------------------------------- Pinpoint customers who are looking for what you sell. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From TAYLORS <@t> DAL.CA Wed Oct 10 14:01:06 2007 From: TAYLORS <@t> DAL.CA (Sean Taylor) Date: Wed Oct 10 14:01:11 2007 Subject: [Histonet] Fixation of Skin biopsies. Message-ID: <20071010160106.25b71k05osfcokkc@my6.dal.ca> Hello histonetters I have been preparing humna skin biopsies for PGP9.5 immunohistochemistry. The Biopsies are fixed in 2% PLP fixative and cryoprotected in 20% glycerol/sorennsens buffer. After cutting several specimens on a cryostat they appear to be insufficinetly fixed. Can tissues that has been cyroprotected be brought back ro Sorennsens buffered and refixed? Any suggestions would be appreciated. Sean Taylor M.D., M.Sc. Department of Neurology Kingston General Hospital 613-546-2550 From carl.hobbs <@t> kcl.ac.uk Wed Oct 10 14:21:15 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Wed Oct 10 14:21:36 2007 Subject: [Histonet] Re: PTEN Message-ID: <003701c80b72$ba64bac0$4101a8c0@carlba65530bda> Well, PTEN Abs have been around for quite a while. Tried a few myself. Which particular anti PTEN Ab are you referring to? C From TJJ <@t> Stowers-Institute.org Wed Oct 10 14:22:38 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Wed Oct 10 14:23:05 2007 Subject: [Histonet] Re: Need help with ISH Message-ID: Naira, There is a lot of information that we need to know before we can really help you with this. Why are you using a tyramide amplification system? Because your target has known low expression? Or because that's what you saw in a paper somewhere? Very high background staining is a usual result from using amplification. Do you actually need amplification? Don't use it if you don't need it. When doing ISH on cells, the formalin fixed cells will need to be permeabilized prior to doing any intracellular staining. It's possible that you have permeabilized the cellular membrane with methanol and acetone. I prefer using detergent solutions to do this, with either Saponin or Triton X-100. It looks like you might be able to simplify your protocol depending on what you are trying to demonstrate. Regardless, unless you can get absolutely negative results with no probe or no antibody, your test samples are a risk for misinterpretation. A typical protocol looks something like this: Cells are fixed, then washed in buffer. Permeabilized with 0.3% Triton x-100 15 minutes. Enzyme digestion (pronase, proteinase K) improves penetration and removes associated proteins. Stabilize cells with a post-fix in 4% PFA in PBS for 5 minutes Optional - Acetylation with acetic anhydride in TEA buffer Pre-hybe in hybridization buffer (no probe) Hybridization in hybe buffer containing probe (time and temperature determined by probe type and composition) Stringency washes to remove unbound probes (concentration and temperature of SSC determined by degree of stringency needed) Detection system depends on what the probe label is - streptavidin AP or HRP if probe is biotinylated, or protein block followed by anti-dig antibody (labeled with AP or HRP) if probe is labeled with digoxigenin. Appropriate chromogen. Not mentioned above are the buffer rinses between steps (no rinse between pre-hybe and probe hybridization). If you follow a plan such as above and have no background (low noise) and strong signal, then you need no further amplification. I recommend reading as much as you can about the ISH process to help you understand what each step in the protocol does. Good luck! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From moran.elish <@t> gmail.com Wed Oct 10 15:01:38 2007 From: moran.elish <@t> gmail.com (Moran Elishmereni) Date: Wed Oct 10 15:01:45 2007 Subject: [Histonet] stain living cells - what dye doesn't kill the cells? Message-ID: <4a722ef70710101301k2a3cfc0fj639a66b90cff567d@mail.gmail.com> Dear Histonetters, Does anyone know of a dye/stain (fluorescent or non-fluorescent) that does not kill cells? I want to do some live-imaging of live primary mast cells, but be able to distinguish them from other cells in a certain mix. So I need to stain them- but not affect there viability/function. I know that this isnt exactly the forum for this question, but I'm hoping to get some suggestions anyway. Many thanks, Moran -- Moran Elishmereni Department of Pharmacology and Experimental Therapeutics School of Pharmacy, Faculty of Medicine The Hebrew University of Jerusalem POB 12065 Jerusalem 91120, ISRAEL Tel: 972-2-675-8746 Fax: 972-2-675-8144 Email: moran.elish@gmail.com From rjbuesa <@t> yahoo.com Wed Oct 10 15:14:33 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Oct 10 15:14:42 2007 Subject: [Histonet] stain living cells - what dye doesn't kill the cells? In-Reply-To: <4a722ef70710101301k2a3cfc0fj639a66b90cff567d@mail.gmail.com> Message-ID: <253114.92698.qm@web61217.mail.yahoo.com> Moran: Your question belongs to the realm of "supravital" or "vital" stains, i.e. those able to accomplish exactly what you want to do. I always used neutral red in dilutions between 1:10,000 and 1:50,000 depending on the subject . The idea being to let the living cells to incorporate and concentrate the dye in a way that they survive and become visible. Now a question: why you don't use a phase or an interference optical system to view the cells, alive and without staining them? Ren? J. Moran Elishmereni wrote: Dear Histonetters, Does anyone know of a dye/stain (fluorescent or non-fluorescent) that does not kill cells? I want to do some live-imaging of live primary mast cells, but be able to distinguish them from other cells in a certain mix. So I need to stain them- but not affect there viability/function. I know that this isnt exactly the forum for this question, but I'm hoping to get some suggestions anyway. Many thanks, Moran -- Moran Elishmereni Department of Pharmacology and Experimental Therapeutics School of Pharmacy, Faculty of Medicine The Hebrew University of Jerusalem POB 12065 Jerusalem 91120, ISRAEL Tel: 972-2-675-8746 Fax: 972-2-675-8144 Email: moran.elish@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better Globetrotter. Get better travel answers from someone who knows. Yahoo! Answers - Check it out. From oshel1pe <@t> cmich.edu Wed Oct 10 15:22:34 2007 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Wed Oct 10 15:22:47 2007 Subject: [Histonet] stain living cells - what dye doesn't kill the cells? In-Reply-To: <4a722ef70710101301k2a3cfc0fj639a66b90cff567d@mail.gmail.com> References: <4a722ef70710101301k2a3cfc0fj639a66b90cff567d@mail.gmail.com> Message-ID: Try 0.01% aqueous Neutral Red. Phil >Dear Histonetters, > >Does anyone know of a dye/stain (fluorescent or non-fluorescent) that does >not kill cells? I want to do some live-imaging of live primary mast cells, >but be able to distinguish them from other cells in a certain mix. So I need >to stain them- but not affect there viability/function. >I know that this isnt exactly the forum for this question, but I'm hoping to >get some suggestions anyway. >Many thanks, >Moran > >-- >Moran Elishmereni > >Department of Pharmacology and Experimental Therapeutics >School of Pharmacy, Faculty of Medicine >The Hebrew University of Jerusalem >POB 12065 >Jerusalem 91120, ISRAEL >Tel: 972-2-675-8746 >Fax: 972-2-675-8144 >Email: moran.elish@gmail.com >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 From jkiernan <@t> uwo.ca Wed Oct 10 15:25:49 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Oct 10 15:25:53 2007 Subject: [Histonet] stain living cells - what dye doesn't kill the cells? In-Reply-To: <4a722ef70710101301k2a3cfc0fj639a66b90cff567d@mail.gmail.com> References: <4a722ef70710101301k2a3cfc0fj639a66b90cff567d@mail.gmail.com> Message-ID: Neutral red is OK. So is janus green B. Toluidine blue will stain the granules (metachromatically), but it is likely to disrupt the mast cells. See H. Selye (1965) The Mast Cells. Washington: Butterworths, pp. 63-66. There's lots of literature on vital staining of mast cells, dating back as far as 1895. John Kiernan Anatomy, UWO London, Canada --- ----- Original Message ----- From: Moran Elishmereni Date: Wednesday, October 10, 2007 16:05 Subject: [Histonet] stain living cells - what dye doesn't kill the cells? To: histonet@lists.utsouthwestern.edu > Dear Histonetters, > > Does anyone know of a dye/stain (fluorescent or non-fluorescent) > that does > not kill cells? I want to do some live-imaging of live primary > mast cells, > but be able to distinguish them from other cells in a certain > mix. So I need > to stain them- but not affect there viability/function. > I know that this isnt exactly the forum for this question, but > I'm hoping to > get some suggestions anyway. > Many thanks, > Moran > > -- > Moran Elishmereni > > Department of Pharmacology and Experimental Therapeutics > School of Pharmacy, Faculty of Medicine > The Hebrew University of Jerusalem > POB 12065 > Jerusalem 91120, ISRAEL > Tel: 972-2-675-8746 > Fax: 972-2-675-8144 > Email: moran.elish@gmail.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Kathy.Abels <@t> sial.com Wed Oct 10 16:01:45 2007 From: Kathy.Abels <@t> sial.com (Kathy Abels) Date: Wed Oct 10 16:02:20 2007 Subject: [Histonet] Kathy Abels/ops/diag/sial is out of the office. Message-ID: I will be out of the office starting 10/09/2007 and will not return until 10/15/2007. I will respond to your message when I return. For urgent matters, Please contact Leigh Gaskill. This message and any files transmitted with it are the property of Sigma-Aldrich Corporation, are confidential, and are intended solely for the use of the person or entity to whom this e-mail is addressed. If you are not one of the named recipient(s) or otherwise have reason to believe that you have received this message in error, please contact the sender and delete this message immediately from your computer. Any other use, retention, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. From Jonathan.Arzt <@t> ARS.USDA.GOV Wed Oct 10 18:44:22 2007 From: Jonathan.Arzt <@t> ARS.USDA.GOV (Arzt, Jonathan) Date: Wed Oct 10 18:44:53 2007 Subject: [Histonet] BIODEFENSE (HT/HTL) POSITION (NY/CT) NOW POSTED Message-ID: <6B4AF69268EFAE4C80A786BA99F7A6670152FFEF@MD-MAIL-01.ARSNET.ARS.USDA.GOV> BIODEFENSE (HT/HTL) POSITION AVAILABLE (NY/CT) This position is currently advertised at: www.usajobs.com as announcement #: ARS-X8E-0001 . Only accepting applications through 10/22/07. A unique, full-time, permanent histotech. position is immediately available and advertised by the pathology division of the USDA, Animal Research Service on Plum Island, NY. The position will be offered at the grades of GS-7/9/11 conferring salary of $39K-$76K commensurate with experience and training. Benefits and promotion potential are excellent. Licensed HT and HTL preferred, but unlicensed candidates with appropriate experience will be considered and are encouraged to apply. The primary mission of the research group is investigation of veterinary diseases of potential threat to US agriculture interests. Currently the greatest emphases are directed towards characterizing the pathogenesis of foot-and-mouth disease and classical swine fever. The Plum Island Animal Disease Center is located off the East end of Long Island, NY. Transport to and from the island is provided free to employees aboard government-operated ferries servicing Orient, NY and Old Saybrook, CT. Rural and suburban communities are abundant near both the NY and CT sides of the ferries with access to scenic beaches and vineyards. New York City and Boston are both within 2-4hrs drive. Activities will be varied but will include conventional histotech work with paraffin-embedded and frozen tissues, immunohistochemistry, in-situ hybridization, confocal microscopy, and assistance with animal necropsies and sample collection. For more information, email to: Jonathan.Arzt@ARS.USDA.GOV If applying, please email to this address directly in addition to filing the application through USAJOBS. Note: this is an informational notice only and does not constitute an official advertisement of any position. From mtarango <@t> nvcancer.org Wed Oct 10 19:27:48 2007 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Wed Oct 10 19:28:13 2007 Subject: [Histonet] stain living cells - what dye doesn't kill the cells? In-Reply-To: <4a722ef70710101301k2a3cfc0fj639a66b90cff567d@mail.gmail.com> Message-ID: <5AEC610C1CE02945BD63A395BA763EDE011B6EA8@NVCIEXCH02.NVCI.org> Check out Invitrogen's Qdots, if you're interested in florescence. You could use those. Mark Adam Tarango HT(ASCP) Histology & IHC Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 Direct Line (702) 822-5112 Mobile (702) 759-9229 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Moran Elishmereni Sent: Wednesday, October 10, 2007 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] stain living cells - what dye doesn't kill the cells? Dear Histonetters, Does anyone know of a dye/stain (fluorescent or non-fluorescent) that does not kill cells? I want to do some live-imaging of live primary mast cells, but be able to distinguish them from other cells in a certain mix. So I need to stain them- but not affect there viability/function. I know that this isnt exactly the forum for this question, but I'm hoping to get some suggestions anyway. Many thanks, Moran -- Moran Elishmereni Department of Pharmacology and Experimental Therapeutics School of Pharmacy, Faculty of Medicine The Hebrew University of Jerusalem POB 12065 Jerusalem 91120, ISRAEL Tel: 972-2-675-8746 Fax: 972-2-675-8144 Email: moran.elish@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From talulahgosh <@t> gmail.com Thu Oct 11 01:21:27 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu Oct 11 01:21:30 2007 Subject: [Histonet] Fetal Tissue - In Situ - Fixation Issues In-Reply-To: <223db0850710100748v490f8459v414fbe69deb3165b@mail.gmail.com> References: <223db0850710100748v490f8459v414fbe69deb3165b@mail.gmail.com> Message-ID: Having done both chick and mouse embryonic nervous tissue, I would suggest letting it fix for 2 - 4 hours (2 hrs if it's under E11.5). This may help. If it doesn't, try fixing the embryo longer--perhaps it takes longer for the fix to perfuse as the embryo gets older--remember, there are more tissues for the fix to absorb through. Also, you may want to lower your concentration of proteinase K in your in situ--using it at room temperature for three minutes is always a good digest for our chick embryonic tissue using PK from Roche (embryos fixed from 2 to overnight) , but we have left it as long at five minutes. Note that we do not heat it to 37 degrees Celcius but leave it at room temperature, and make it right before this step. This also brings up the fact that you may be using your proteinase K repeatedly--which is not bad, unless you freeze/thaw it. That is not good for proteinase K--for every aliquot, you should freeze it, then thaw it once (throwing it away afterwards) for optimal results. The next possibility I would consider is that someone is being too rough with your samples. It may sound ridiculous, but I can't tell you how many times that has come into play when processing our slides (can I emphasize GENTLE, DON'T DROP!). Every step requires gentle lifting from the solution, and gentle lowering into the next. Furthermore, use enough solution to cover the slides COMPLETELY AND MORE; use enough solution to at least cover 1 cm of distance between the top of the slide and the meniscus of the liquid level. I haven't calculated some magical number between the two; it seems that a separation affects the way tissue adheres to the slide, as if near a surface tension--my guess only. -- Yog-Sothoth knows the gate. Yog-Sothoth is the gate. Yog-Sothoth is the key and guardian of the gate. Past, present, future, all are one in Yog-Sothoth. He knows where the Old Ones broke through of old, and where They shall break through again. From Kemlo.Rogerson <@t> waht.swest.nhs.uk Thu Oct 11 01:58:13 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Oct 11 01:58:20 2007 Subject: [Histonet] H&E Staining Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222EEF2@wahtntex2.waht.swest.nhs.uk> Not my question; hence my reply that was attached. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Creativity requires the courage to let go of certainties. --Erich Fromm This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From talulahgosh <@t> gmail.com Thu Oct 11 02:58:28 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu Oct 11 02:58:38 2007 Subject: [Histonet] H&E Staining In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBF0222EEF2@wahtntex2.waht.swest.nhs.uk> References: <86ADE4EB583CE64799A9924684A0FBBF0222EEF2@wahtntex2.waht.swest.nhs.uk> Message-ID: Hello, I appreciate hearing the answers to the questions asked, but sometimes I can't find them in my mail. Could we please send a reply with a response including the question? I don't mind, nor do I think anyone else minds, that the question is copied from another email or quoted. I also don't mind a direct fragmental quote. I can also blame my absence of mind or email account, but I hope I don't have to :) On that note, what does this reply refer to--eosin as alcoholic or aqueous? (seems to be the likely topic) On 10/11/07, Kemlo Rogerson wrote: > > Not my question; hence my reply that was attached. > > Kemlo Rogerson > Pathology Manager > DD 01934 647057 or extension 3311 > Mob 07749 754194; Pager 07659 597107; > > Creativity requires the courage to let go of certainties. --Erich Fromm > > -- Yog-Sothoth knows the gate. Yog-Sothoth is the gate. Yog-Sothoth is the key and guardian of the gate. Past, present, future, all are one in Yog-Sothoth. He knows where the Old Ones broke through of old, and where They shall break through again. From GAshton <@t> picr.man.ac.uk Thu Oct 11 05:59:44 2007 From: GAshton <@t> picr.man.ac.uk (Garry Ashton) Date: Thu Oct 11 06:00:13 2007 Subject: [Histonet] LCM Message-ID: Hi, I was just wondering, does anybody have any opinions on the laser capture microdissection system they are currently using, and what is currently available, together with the pros and cons Many thanks in advance Garry PICR UK -------------------------------------------------------- This email is confidential and intended solely for the use of the person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Paterson Institute for Cancer Research or the University of Manchester. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From kmerriam2003 <@t> yahoo.com Thu Oct 11 06:54:13 2007 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Thu Oct 11 06:54:18 2007 Subject: [Histonet] LCM Message-ID: <253023.49492.qm@web50302.mail.re2.yahoo.com> Garry, I have not done LCM in a couple of years, but I have used both the Arcturus Autopix and the Arcturus Vertitas. They are both good, but the Vertias is much easier to use and has the 2 major advantages: "laser cutting", which allows you to cut out a larger piece of the tissue very quickly (much better than having the laser fire over an entire large area). This feature is not available on the Autopix. live-image mark-up; with the Autopix, you need to take a picture, mark up what you want to capture and then tell the machine to go. You can mark your slides up live on-screen with the Veritas (it's much quicker, I can remember taking 10 or 20 pictures per slide, which could get time consuming). The quicker everything can get done, the better quality your sample will be. It all depends on the type of samples you are planning to work with, if they are large or require large areas, the Veritas is well worth the extra money. In addition, Arcturus is one of only a (very) small handful of companies that I would say has excellent customer service. Their field technicians were always available to come and help me. As you are in the UK, you would need to check to see what kind of local support they offer. Just my 2-cents. Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA ----- Original Message ---- From: Garry Ashton To: histonet@lists.utsouthwestern.edu Sent: Thursday, October 11, 2007 6:59:44 AM Subject: [Histonet] LCM Hi, I was just wondering, does anybody have any opinions on the laser capture microdissection system they are currently using, and what is currently available, together with the pros and cons Many thanks in advance Garry PICR UK -------------------------------------------------------- This email is confidential and intended solely for the use of the person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Paterson Institute for Cancer Research or the University of Manchester. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Be a better Globetrotter. Get better travel answers from someone who knows. Yahoo! Answers - Check it out. http://answers.yahoo.com/dir/?link=list&sid=396545469 From relia1 <@t> earthlink.net Thu Oct 11 07:10:05 2007 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Oct 11 07:10:16 2007 Subject: [Histonet] RELIA's Histology Careers Bulletin 10/11/07 Message-ID: Hi Histonetters! I hope you are enjoying a wonderful Autumn. Halloween is just around the corner TRICK or TREAT!!!!! The TRICK is finding the right job opportunity for you. And the TREAT is with my help it won?t be spooky at all! I will help you with your resume, coach you through the interview and offer process and refer you to positions based on the critieria you give me. All of the opportunities I work with are fulltime 40 hour per week positions with top hospitals, labs and doctors offices. My clients offer excellent compensation including competitive salaries, great benefits and relocation assistance. My services are FREE of charge to you. All of my fees are paid by the facilities that I represent Here is a list of my current openings HISTOLOGY MANAGEMENT: 1. Dallas, TX 2. Santa Barbara, CA 3. Valencia, CA 4. Cape Cod, MA 5. Chicago, IL 6. Pathology Assistant ? Los Angeles, CA HISTOTECHNICIAN/TECHNOLOGIST: 1. Phoenix, AZ 2. New York, NY 3. San Francisco, CA 4. Los Angeles, CA 5. Valencia, CA 6. Orlando, FL 7. St. Petersburg, FL 8. Fredericksburg, VA 9. Dallas, TX 10. Waco, TX 11. Columbus, OH 12. Falmouth, MA 13. Spokane, WA 14. Atlanta, GA HEADS UP !! These are areas that some of my clients project a need in the near future. New Braunsfel, TX Kalamazoo, MI Panama City, FL Rochester, MN Denver, CO Columbus, OH If you or any of your friends would like more information on any of these positions. Or if you would like to discuss opportunities in other areas or future job searches please contact me. I would be more than happy to assist you. You can reach me at 866-607-3542 or relia1@earthlink.net I work with facilities nationwide and I will keep your resume confidential. I will only represent you to jobs you tell me you are interested in looking into. Remember It never hurts to keep an eye open even if you are happy in your present job. Happy Halloween!!!!!!!!!!!! Pam ? 866-607-3542 (866-60RELIA) Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.myspace.com/pamatrelia From moran.elish <@t> gmail.com Thu Oct 11 07:13:55 2007 From: moran.elish <@t> gmail.com (Moran Elishmereni) Date: Thu Oct 11 07:14:01 2007 Subject: [Histonet] which mounting media for DAB + toluidine blue staining? Message-ID: <4a722ef70710110513s239abf79tfebc5237253eb705@mail.gmail.com> Hi histonetters, I am staining skin sections for mast cells (toluidine blue) and for an eosinophil protein (using DAB). Can I use entelan as mounting media? It works nicely for the tol blue, but I fear it will ruin the DAB staining. Is there any alternative? Thanks, Moran -- Moran Elishmereni Department of Pharmacology and Experimental Therapeutics School of Pharmacy, Faculty of Medicine The Hebrew University of Jerusalem POB 12065 Jerusalem 91120, ISRAEL Tel: 972-2-675-8746 Fax: 972-2-675-8144 Email: moran.elish@gmail.com From b-frederick <@t> northwestern.edu Thu Oct 11 07:52:21 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Oct 11 07:52:27 2007 Subject: [Histonet] CRYOSTAT TROUBLESHOOTING In-Reply-To: <5F31F38C96781A4FBE3196EBC22D47802E1162@fhosxchmb006.ADVENTISTCORP.NET> Message-ID: <004701c80c05$90371d40$d00f7ca5@lurie.northwestern.edu> I have found that the reason the tissue falls off from a precooled chuck, such as leaving them premade and in the cryostat overnight, is because of the defrost cycle we all have set to run during the night. As a result, we keep the chucks in the cryostat un made and only make them in the morning. The chucks can also be placed in the cryostat first thing to cool down and then the OCT added etc. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonner, Janet Sent: Tuesday, October 09, 2007 1:12 PM To: Matt Bancroft; Sebree Linda A.; mstone@cmhlink.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CRYOSTAT TROUBLESHOOTING We also use the warm chucks. Without exception, the OCT chunks off the chuck when the chuck is pre-cooled. We have chucks of all ages and stages of use. We only use the large chucks and clean them in a virex soak and a wire brush. Both of these last specifications may be factors! For those of you who precool your chucks, what size/type of chuck are you using and what do you clean them with? The chucks come out so much neater if the chuck is cooler before the OCT is applied! Thank you! Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Matt Bancroft Sent: Tue 10/9/2007 11:52 AM To: Sebree Linda A.; mstone@cmhlink.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CRYOSTAT TROUBLESHOOTING I am a manger of a mohs lab, I use room temp molds to keep the OCT from popping off the chuck. Matt Bancroft HT (ASCP) "Sebree Linda A." wrote: Marcy, I was always taught to apply the OCT to a RT chuck, put in the cryostat and when you start to see the OCT turn white, i.e. the OCT in the grooves is starting to freeze, add the tissue and weight down with the cold heat extractor. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MARCELLYN STONE Sent: Tuesday, October 09, 2007 9:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CRYOSTAT TROUBLESHOOTING Hello.. I am looking for some help. The O.C.T. embedded tissue is falling of the chuck when we try to section. Does anyone have any ideas to stop this? We have tried new O.C.T., cleaning chucks, advancing slower. Please help. Thanks Marcy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Moody friends. Drama queens. Your life? Nope! - their life, your story. Play Sims Stories at Yahoo! Games. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmiller <@t> physlab.com Thu Oct 11 09:04:56 2007 From: cmiller <@t> physlab.com (Cheri Miller) Date: Thu Oct 11 09:05:12 2007 Subject: [Histonet] Iron Staining of Bone Marrows In-Reply-To: <004801c80b5a$5c520360$6a01a8c0@CHERYLSLAPTOP> References: <970248.21844.qm@web58911.mail.re1.yahoo.com> <004801c80b5a$5c520360$6a01a8c0@CHERYLSLAPTOP> Message-ID: <005001c80c0f$b5edea50$3402a8c0@plab.local> Well said! We gave up and did them by hand. They always work well and its so easy to do by hand Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl R. Kerry Sent: Wednesday, October 10, 2007 11:27 AM To: 'Histonet' Subject: RE: [Histonet] Iron Staining of Bone Marrows Hey Schaundra- Have you talked with Ventana tech support? They've probably dealt with similar issues from other clients and would know right off the bat how to help if there is help to be had. If there isn't there always the old-fashioned alternative to fix this for your Path... I know this isn't what you want to hear, but sometimes automation cannot duplicate what we can produce by hand. If you try what they suggest and he's not getting what he needs to adequately diagnose the patient--he needs to serve the patient's best interest (an accurate diagnosis). You may need to pull these off the machine. Running them by hand isn't all that hard OR time-consuming if your reagents are at hand, and you do end up with a better product. My two cents having been in your shoes in the past... Cheryl Cheryl R. Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab, one great tech at a time. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Schaundra Walton Sent: Wednesday, October 10, 2007 11:04 AM To: Histonet Subject: [Histonet] Iron Staining of Bone Marrows We use a Vetana NEXUS special stainer and stain our bone marrow aspirates and smears with Ventana's iron kit. One of our pathologists has complained (repeatedly) that our irons aren't staining like he expects. Has anyone else had trouble with Ventana's iron kit? Is anyone using a different protocol? Any advise would be greatly appreciated. Schaundra Walton BS HTL(ASCP) Swedish American Hospital 1401 E. State St. Rockford, IL 61104 --------------------------------- Pinpoint customers who are looking for what you sell. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From jkiernan <@t> uwo.ca Thu Oct 11 09:38:16 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Thu Oct 11 09:39:19 2007 Subject: [Histonet] which mounting media for DAB + toluidine blue staining? In-Reply-To: <4a722ef70710110513s239abf79tfebc5237253eb705@mail.gmail.com> References: <4a722ef70710110513s239abf79tfebc5237253eb705@mail.gmail.com> Message-ID: Yes. Use Entellan or any other resinous mounting medium. Nothing removes the oxidation product of DAB. John Kiernan Anatomy, UWO London, Canada --- ----- Original Message ----- From: Moran Elishmereni Date: Thursday, October 11, 2007 8:16 Subject: [Histonet] which mounting media for DAB + toluidine blue staining? To: histonet@lists.utsouthwestern.edu > Hi histonetters, > > I am staining skin sections for mast cells (toluidine blue) and > for an > eosinophil protein (using DAB). Can I use entelan as mounting > media? It > works nicely for the tol blue, but I fear it will ruin the DAB > staining. Is > there any alternative? > > Thanks, > Moran > > -- > Moran Elishmereni > > Department of Pharmacology and Experimental Therapeutics > School of Pharmacy, Faculty of Medicine > The Hebrew University of Jerusalem > POB 12065 > Jerusalem 91120, ISRAEL > Tel: 972-2-675-8746 > Fax: 972-2-675-8144 > Email: moran.elish@gmail.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From akbitting <@t> geisinger.edu Thu Oct 11 09:42:52 2007 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Thu Oct 11 09:43:06 2007 Subject: [Histonet] renal immunofluorescence Message-ID: <470DFE2C020000C900010287@GHSGWIANW5V.GEISINGER.EDU> Where can I buy FITC-conjugated Lambda for direct immunofluorescent staining of renal bxs? Can someone recommend a reliable company? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From dshtilki <@t> uci.edu Thu Oct 11 10:18:31 2007 From: dshtilki <@t> uci.edu (Shtilkind, Dmitriy) Date: Thu Oct 11 10:18:37 2007 Subject: [Histonet] FISH staining Message-ID: <2937D5939ABCCA4F96C14BBF3E463A1C03859D1B@GUS.hs.uci.edu> We are considering doing FISH in house. I would like to get some opinions about equipment and all problems that go with that. Dako vs Ventana etc... Dmitriy Shtilkind, HTL (ASCP) Histology Supervisor UCI Medical Center Orange, CA, 92868 (714) 456-8731 dshtilki@uci.edu From dshtilki <@t> uci.edu Thu Oct 11 10:51:14 2007 From: dshtilki <@t> uci.edu (Shtilkind, Dmitriy) Date: Thu Oct 11 10:51:17 2007 Subject: [Histonet] FISH test Message-ID: <2937D5939ABCCA4F96C14BBF3E463A1C03859D4C@GUS.hs.uci.edu> We are considering doing FISH in house. I would like to get some opinions about equipment and all problems that go with that. Dako vs Ventana etc... Dmitriy Shtilkind, HTL (ASCP) Histology Supervisor UCI Medical Center Orange, CA, 92868 (714) 456-8731 dshtilki@uci.edu From rjbuesa <@t> yahoo.com Thu Oct 11 10:53:48 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 11 10:53:55 2007 Subject: [Histonet] which mounting media for DAB + toluidine blue staining? In-Reply-To: <4a722ef70710110513s239abf79tfebc5237253eb705@mail.gmail.com> Message-ID: <107182.50125.qm@web61219.mail.yahoo.com> Moran: Once the DAB has reacted (oxidized) it is permanent, no matter which mounting medium you use. Ren? J. Moran Elishmereni wrote: Hi histonetters, I am staining skin sections for mast cells (toluidine blue) and for an eosinophil protein (using DAB). Can I use entelan as mounting media? It works nicely for the tol blue, but I fear it will ruin the DAB staining. Is there any alternative? Thanks, Moran -- Moran Elishmereni Department of Pharmacology and Experimental Therapeutics School of Pharmacy, Faculty of Medicine The Hebrew University of Jerusalem POB 12065 Jerusalem 91120, ISRAEL Tel: 972-2-675-8746 Fax: 972-2-675-8144 Email: moran.elish@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Fussy? Opinionated? Impossible to please? Perfect. Join Yahoo!'s user panel and lay it on us. From rjbuesa <@t> yahoo.com Thu Oct 11 10:55:12 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 11 10:55:16 2007 Subject: [Histonet] FISH test In-Reply-To: <2937D5939ABCCA4F96C14BBF3E463A1C03859D4C@GUS.hs.uci.edu> Message-ID: <178655.85336.qm@web61221.mail.yahoo.com> Try to contact Abbot to see their products. Ren? J. "Shtilkind, Dmitriy" wrote: We are considering doing FISH in house. I would like to get some opinions about equipment and all problems that go with that. Dako vs Ventana etc... Dmitriy Shtilkind, HTL (ASCP) Histology Supervisor UCI Medical Center Orange, CA, 92868 (714) 456-8731 dshtilki@uci.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better Globetrotter. Get better travel answers from someone who knows. Yahoo! Answers - Check it out. From LSebree <@t> uwhealth.org Thu Oct 11 12:43:27 2007 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Thu Oct 11 12:43:36 2007 Subject: FW: [Histonet] renal immunofluorescence Message-ID: Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: Normington Lacy Sent: Thursday, October 11, 2007 11:26 AM To: Sebree Linda A. Subject: RE: [Histonet] renal immunofluorescence I use DakoCytomation. The address is 6392 Via Real, Carpinteria, CA 93013. The reference number is F0199. I dilute the antibody using their Background Reducing Diluent, reference number S3022. It is important to remember that when using in conjunction with Kappa, that the protein ratio is taken into account for dilution purposes. If my procedure is needed, let me know. Lacy -----Original Message----- From: Sebree Linda A. Sent: Thursday, October 11, 2007 10:29 AM To: Normington Lacy Subject: FW: [Histonet] renal immunofluorescence Lacy, Is this something you can help with? Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Thursday, October 11, 2007 9:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] renal immunofluorescence Where can I buy FITC-conjugated Lambda for direct immunofluorescent staining of renal bxs? Can someone recommend a reliable company? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dorothy.L.Webb <@t> HealthPartners.Com Thu Oct 11 12:48:24 2007 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Thu Oct 11 12:48:33 2007 Subject: [Histonet] Iron staining In-Reply-To: <01MMEA4J9C9A000FE9@Dino.HealthPartners.Com> Message-ID: <0E394B648E5284478A6CCB78E5AFDA27056351D8@hpes1.HealthPartners.int> We have the Nexus special stainer and quit using the iron stain 2 years ago, as it did not give true staining on the iron for our bone marrows either. Since we went back to doing them by hand, which is very easy, things are great in the world of FE staining! We do all of our irons by hand as long as we have to have the reagents for the bone marrows and it works well for us! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, October 11, 2007 12:13 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 47, Issue 13 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Iron Staining of Bone Marrows (Cheri Miller) 2. Re: which mounting media for DAB + toluidine blue staining? (John Kiernan) 3. renal immunofluorescence (Angela Bitting) 4. FISH staining (Shtilkind, Dmitriy) 5. FISH test (Shtilkind, Dmitriy) 6. Re: which mounting media for DAB + toluidine blue staining? (Rene J Buesa) 7. Re: FISH test (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Thu, 11 Oct 2007 09:04:56 -0500 From: "Cheri Miller" Subject: RE: [Histonet] Iron Staining of Bone Marrows To: "'Cheryl R. Kerry'" , "'Histonet'" Message-ID: <005001c80c0f$b5edea50$3402a8c0@plab.local> Content-Type: text/plain; charset="us-ascii" Well said! We gave up and did them by hand. They always work well and its so easy to do by hand Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl R. Kerry Sent: Wednesday, October 10, 2007 11:27 AM To: 'Histonet' Subject: RE: [Histonet] Iron Staining of Bone Marrows Hey Schaundra- Have you talked with Ventana tech support? They've probably dealt with similar issues from other clients and would know right off the bat how to help if there is help to be had. If there isn't there always the old-fashioned alternative to fix this for your Path... I know this isn't what you want to hear, but sometimes automation cannot duplicate what we can produce by hand. If you try what they suggest and he's not getting what he needs to adequately diagnose the patient--he needs to serve the patient's best interest (an accurate diagnosis). You may need to pull these off the machine. Running them by hand isn't all that hard OR time-consuming if your reagents are at hand, and you do end up with a better product. My two cents having been in your shoes in the past... Cheryl Cheryl R. Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab, one great tech at a time. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Schaundra Walton Sent: Wednesday, October 10, 2007 11:04 AM To: Histonet Subject: [Histonet] Iron Staining of Bone Marrows We use a Vetana NEXUS special stainer and stain our bone marrow aspirates and smears with Ventana's iron kit. One of our pathologists has complained (repeatedly) that our irons aren't staining like he expects. Has anyone else had trouble with Ventana's iron kit? Is anyone using a different protocol? Any advise would be greatly appreciated. Schaundra Walton BS HTL(ASCP) Swedish American Hospital 1401 E. State St. Rockford, IL 61104 --------------------------------- Pinpoint customers who are looking for what you sell. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ------------------------------ Message: 2 Date: Thu, 11 Oct 2007 10:38:16 -0400 From: John Kiernan Subject: Re: [Histonet] which mounting media for DAB + toluidine blue staining? To: Moran Elishmereni Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii Yes. Use Entellan or any other resinous mounting medium. Nothing removes the oxidation product of DAB. John Kiernan Anatomy, UWO London, Canada --- ----- Original Message ----- From: Moran Elishmereni Date: Thursday, October 11, 2007 8:16 Subject: [Histonet] which mounting media for DAB + toluidine blue staining? To: histonet@lists.utsouthwestern.edu > Hi histonetters, > > I am staining skin sections for mast cells (toluidine blue) and > for an > eosinophil protein (using DAB). Can I use entelan as mounting > media? It > works nicely for the tol blue, but I fear it will ruin the DAB > staining. Is > there any alternative? > > Thanks, > Moran > > -- > Moran Elishmereni > > Department of Pharmacology and Experimental Therapeutics School of > Pharmacy, Faculty of Medicine The Hebrew University of Jerusalem > POB 12065 > Jerusalem 91120, ISRAEL > Tel: 972-2-675-8746 > Fax: 972-2-675-8144 > Email: moran.elish@gmail.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 3 Date: Thu, 11 Oct 2007 10:42:52 -0400 From: "Angela Bitting" Subject: [Histonet] renal immunofluorescence To: Message-ID: <470DFE2C020000C900010287@GHSGWIANW5V.GEISINGER.EDU> Content-Type: text/plain; charset=US-ASCII Where can I buy FITC-conjugated Lambda for direct immunofluorescent staining of renal bxs? Can someone recommend a reliable company? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ------------------------------ Message: 4 Date: Thu, 11 Oct 2007 08:18:31 -0700 From: "Shtilkind, Dmitriy" Subject: [Histonet] FISH staining To: Message-ID: <2937D5939ABCCA4F96C14BBF3E463A1C03859D1B@GUS.hs.uci.edu> Content-Type: text/plain; charset="us-ascii" We are considering doing FISH in house. I would like to get some opinions about equipment and all problems that go with that. Dako vs Ventana etc... Dmitriy Shtilkind, HTL (ASCP) Histology Supervisor UCI Medical Center Orange, CA, 92868 (714) 456-8731 dshtilki@uci.edu ------------------------------ Message: 5 Date: Thu, 11 Oct 2007 08:51:14 -0700 From: "Shtilkind, Dmitriy" Subject: [Histonet] FISH test To: Message-ID: <2937D5939ABCCA4F96C14BBF3E463A1C03859D4C@GUS.hs.uci.edu> Content-Type: text/plain; charset="us-ascii" We are considering doing FISH in house. I would like to get some opinions about equipment and all problems that go with that. Dako vs Ventana etc... Dmitriy Shtilkind, HTL (ASCP) Histology Supervisor UCI Medical Center Orange, CA, 92868 (714) 456-8731 dshtilki@uci.edu ------------------------------ Message: 6 Date: Thu, 11 Oct 2007 08:53:48 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] which mounting media for DAB + toluidine blue staining? To: Moran Elishmereni , histonet@lists.utsouthwestern.edu Message-ID: <107182.50125.qm@web61219.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Moran: Once the DAB has reacted (oxidized) it is permanent, no matter which mounting medium you use. Ren? J. Moran Elishmereni wrote: Hi histonetters, I am staining skin sections for mast cells (toluidine blue) and for an eosinophil protein (using DAB). Can I use entelan as mounting media? It works nicely for the tol blue, but I fear it will ruin the DAB staining. Is there any alternative? Thanks, Moran -- Moran Elishmereni Department of Pharmacology and Experimental Therapeutics School of Pharmacy, Faculty of Medicine The Hebrew University of Jerusalem POB 12065 Jerusalem 91120, ISRAEL Tel: 972-2-675-8746 Fax: 972-2-675-8144 Email: moran.elish@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Fussy? Opinionated? Impossible to please? Perfect. Join Yahoo!'s user panel and lay it on us. ------------------------------ Message: 7 Date: Thu, 11 Oct 2007 08:55:12 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] FISH test To: "Shtilkind, Dmitriy" , histonet@lists.utsouthwestern.edu Message-ID: <178655.85336.qm@web61221.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Try to contact Abbot to see their products. Ren? J. "Shtilkind, Dmitriy" wrote: We are considering doing FISH in house. I would like to get some opinions about equipment and all problems that go with that. Dako vs Ventana etc... Dmitriy Shtilkind, HTL (ASCP) Histology Supervisor UCI Medical Center Orange, CA, 92868 (714) 456-8731 dshtilki@uci.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better Globetrotter. Get better travel answers from someone who knows. Yahoo! Answers - Check it out. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 47, Issue 13 **************************************** ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From TMcNemar <@t> lmhealth.org Thu Oct 11 13:13:08 2007 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Thu Oct 11 13:13:15 2007 Subject: [Histonet] Flooring recommendations... Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F4D6@lmhsmail.lmhealth.org> Hello all, It looks like we will be moving into newly renovated space. I was wondering if anyone had any recommendations regarding non-slip flooring for the cutting room. If there's a particular type, brand, manufacturer, etc., I'd sure like to hear about it. Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org From tkngflght <@t> yahoo.com Thu Oct 11 13:27:54 2007 From: tkngflght <@t> yahoo.com (Cheryl R. Kerry) Date: Thu Oct 11 13:27:53 2007 Subject: [Histonet] Flooring recommendations... In-Reply-To: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F4D6@lmhsmail.lmhealth.org> Message-ID: <008f01c80c34$7126a590$6a01a8c0@CHERYLSLAPTOP> Hey Tom-- Concrete--rough finish but sealed with one of the new 'stain' type of finishes. They use these finishes in commercial garages and they're chemical resistant and TOUGH. The roughened concrete works for sure footing even with motor oil and the colors and designs you can create with the products are simply beautiful. If you pour it with a lip you can flood the place with no harm to the substrate or cabinetry. Run it up a wall and you can pressure-wash the room if you have a drain! Unfortunately you can't get these but through a distributor or an application company so Google around your area to find the supplier. They should have local installations you can look at or at least a photo gallery. Pretty, tough, safe, durable...sounds like what I want in my kitchen!! Cheryl Cheryl R. Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab, one great tech at a time. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Thursday, October 11, 2007 1:13 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Flooring recommendations... Hello all, It looks like we will be moving into newly renovated space. I was wondering if anyone had any recommendations regarding non-slip flooring for the cutting room. If there's a particular type, brand, manufacturer, etc., I'd sure like to hear about it. Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From petepath <@t> yahoo.com Thu Oct 11 13:31:34 2007 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Thu Oct 11 13:31:38 2007 Subject: [Histonet] CRYOSTAT TROUBLESHOOTING Message-ID: <585724.79940.qm@web45107.mail.sp1.yahoo.com> For the last 5 years I have been promoting a system of face down embedding tissue for frozen section which I developed over my 25 year surgical pathology practice. The system is rapid, very precise and wastes very little tissue. I am all but exhausted devoting most of my vacation and free time to bring this sytem to my colleagues at very limited profit.My chucks are made y.to be used cold with deep wide waffle pattern grooves. They are made of stainless so they function as a heat extractor. With the exception of Leica, I am quite surprised and saddened by the lack of interest in our cryostat manufacturers to explore a new technology which could benefit their customers. I believe they are hoping I fade away. If you want to improve your frozen section practice, I suggest you seek out this system. I would be happy to direct you to it, but the last time I offered help, one of the vendors complained I was breaking a vendor rule. Stephen From kmerriam2003 <@t> yahoo.com Thu Oct 11 13:35:08 2007 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Thu Oct 11 13:35:13 2007 Subject: [Histonet] Zebrafish Atlas Message-ID: <768146.8396.qm@web50309.mail.re2.yahoo.com> Hi, Can anyone recommend a Zebrafish histology atlas? I did a quick Amazon.com search but could not come up with anything. Thanks, Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA ____________________________________________________________________________________ Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. http://farechase.yahoo.com/ From dshtilki <@t> uci.edu Thu Oct 11 13:44:09 2007 From: dshtilki <@t> uci.edu (Shtilkind, Dmitriy) Date: Thu Oct 11 13:44:14 2007 Subject: [Histonet] Histotech wanted Message-ID: <2937D5939ABCCA4F96C14BBF3E463A1C03859E7D@GUS.hs.uci.edu> We have one opening for an experienced histotech at Univercity of California, Irvine. Here is the link http://www.ucihs.uci.edu/hris/bulletin/btJobView.asp?jn=MH43407P&t=Histo technologist%20II&pt=MH&ps= Dmitriy Shtilkind, HTL (ASCP) Histology Supervisor UCI Medical Center Orange, CA, 92868 (714) 456-8731 dshtilki@uci.edu From gvdobbin <@t> ihis.org Thu Oct 11 13:58:42 2007 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Thu Oct 11 13:59:02 2007 Subject: [Histonet] Washed out nuclear staining??? Message-ID: Hi Folks, Mystery to solve. Some of the surgical cases that came off our stainer today had very pale washed out looking nuclei. Our H&E control was fine (ran it twice) so it tells me the problem is either at the source or in the tissue processor. However, since not all cases are affected, I can't see how it could be the processor (VIP 5). So if it is the source, what are we talking about-something other than formalin in their formalin bottles? Contaminated formalin? Insufficient fixation time? Any and all comments will be welcome. Thanks in advance (again!) Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 There is some merit in doing the right thing rather badly, but absolutely none in doing the wrong thing excellently! Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ayant pour objet une personne ou un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer compl?tement de votre syst?me informatique. From lpjones <@t> srhs-pa.org Thu Oct 11 14:04:33 2007 From: lpjones <@t> srhs-pa.org (Jones, Laura) Date: Thu Oct 11 14:04:47 2007 Subject: FW: [Histonet] Re: Anti-parrafin floor Message-ID: <8E7AD740937B954F947F0DB4467EFEE056E9EC@mail.srhs-pa.org> I accidentally deleted the inquiry about flooring, so I don't remember who was asking; but since I've been saving this message for three years (!!!)(in hopes of one day remodeling...) I thought I'd share. Hope it's helpful. Laura -----Original Message----- From: DDittus787@aol.com [mailto:DDittus787@aol.com] Sent: Monday, September 13, 2004 1:49 PM To: BBEIER@kumc.edu Cc: histonet@pathology.swmed.edu Subject: [Histonet] Re: Anti-parrafin floor Barb: the flooring came from Acme Panels-the company is in the UK and can be found on Google, it is anti-solvent, chemical, paraffin,etc. It comes in 3 or 4 colors and everyone loves it. We had a contractor bring it in and install it. dana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hymclab <@t> hyhc.com Thu Oct 11 14:17:03 2007 From: hymclab <@t> hyhc.com (hymclab) Date: Thu Oct 11 14:14:12 2007 Subject: [Possible Spam] [Histonet] CRYOSTAT TROUBLESHOOTING Message-ID: Please contact Dr. Peters to try his system (especially for anyone doing Mohs frozen sections). It is a very quick, easy system to use and your sections come out beautiful every time. And yes, you use the chucks cold and they never come off!!! We have been using his bar and chucks in our Leica Cryocut and old Tissue-Tek cryostat for approximately 3 years with very high success and our Pathologists love how the skin adheres to the metal for a very flat section!!!! If I'm correct Dr. Peters lets you try his system first and pay for it later if you like it (if not, of course return to him). I am in no way associated with Dr. Peters----just a satisfied customer!!!! We had him speak at our Tri-State meeting in Iowa this last spring and it was wonderful!!! Dawn D. Schneider, HT(ASCP) Howard Young Medical Center Woodruff, WI 54568 (715)356-8174 schneiderd@hyhc.com -----Original Message----- From: Stephen Peters M.D. [mailto:petepath@yahoo.com] Sent: Thursday, October 11, 2007 1:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Possible Spam] [Histonet] CRYOSTAT TROUBLESHOOTING For the last 5 years I have been promoting a system of face down embedding tissue for frozen section which I developed over my 25 year surgical pathology practice. The system is rapid, very precise and wastes very little tissue. I am all but exhausted devoting most of my vacation and free time to bring this sytem to my colleagues at very limited profit.My chucks are made y.to be used cold with deep wide waffle pattern grooves. They are made of stainless so they function as a heat extractor. With the exception of Leica, I am quite surprised and saddened by the lack of interest in our cryostat manufacturers to explore a new technology which could benefit their customers. I believe they are hoping I fade away. If you want to improve your frozen section practice, I suggest you seek out this system. I would be happy to direct you to it, but the last time I offered help, one of the vendors complained I was breaking a vendor rule. Stephen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic message and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. Dissemination, forwarding, printing or copying of this message/documents without the consent of the sender is prohibited. From rjbuesa <@t> yahoo.com Thu Oct 11 14:39:48 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 11 14:39:59 2007 Subject: [Histonet] Washed out nuclear staining??? In-Reply-To: Message-ID: <699909.33012.qm@web61219.mail.yahoo.com> Greg: You are right when saying that the VIP is not the culprit, but the temperature at which the slides were dried. You either have two drying ovens (one at more than 60?C) or one batch was subjected to higher temperature that the other for whatever reason. Probably you also used controls already ready to stain and added just before staining the batches, and that could explain why the controls came OK and some slides did not. This problem was dealt with in an article of the Journal of Histotechnology (vol.13, No.2 pp 135-6, 1990). Hope this solved the mistery! Ren? J. Greg Dobbin wrote: Hi Folks, Mystery to solve. Some of the surgical cases that came off our stainer today had very pale washed out looking nuclei. Our H&E control was fine (ran it twice) so it tells me the problem is either at the source or in the tissue processor. However, since not all cases are affected, I can't see how it could be the processor (VIP 5). So if it is the source, what are we talking about-something other than formalin in their formalin bottles? Contaminated formalin? Insufficient fixation time? Any and all comments will be welcome. Thanks in advance (again!) Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 There is some merit in doing the right thing rather badly, but absolutely none in doing the wrong thing excellently! Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ayant pour objet une personne ou un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer compl?tement de votre syst?me informatique. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Need a vacation? Get great deals to amazing places on Yahoo! Travel. From ploykasek <@t> phenopath.com Thu Oct 11 17:53:09 2007 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Fri Oct 12 01:30:41 2007 Subject: [Histonet] IGF antibody Message-ID: Hi all. I'm asking a question for a research colleague. Does anyone have an antibody to IGF-1 (insulin-like growth factor) working in either formalin fixed tissue or frozen tissue? If so, what clone are you using, and what are you using for control tissue? Thank you. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From histology.bc <@t> shaw.ca Thu Oct 11 17:59:34 2007 From: histology.bc <@t> shaw.ca (Paul Bradbury) Date: Fri Oct 12 01:30:44 2007 Subject: [Histonet] CRYOSTAT TROUBLESHOOTING In-Reply-To: <585724.79940.qm@web45107.mail.sp1.yahoo.com> References: <585724.79940.qm@web45107.mail.sp1.yahoo.com> Message-ID: <470EAAD6.6010309@shaw.ca> I have just spent the last few hours doing frozen sections on a number of clinical skin specimens using the procedure that Stephen is talking about. I have been using this approach for well over a year and I love it!! It solves most of the problems routinely associated with doing frozens on skin.The quality of the sections is great, the orientation is quick and easy, and the overall time required is less. Stephen has obviously put a lot of thought and experience into the development of this system ... and it shows. When I first saw the video clip on his web site, I was was skeptical and thought it all looked too easy. I was wrong; it really is that easy! Sure, there is some expense. You need to buy the freezing blocks, heat extractor blocks, and chucks (in a pinch, you can actually use the Microm chucks too). But, if quality of section, ease of use, and time saving are important ... you owe it to yourself to give it a try. I am very surprised to hear that the cryostat manufacturers are not promoting this system. What is there to lose? If a vendor has complained about this system being promoted on the Histonet, too bad! The prime purpose of the Histonet is to provide a forum for histotechnologists, pathologists, researchers, etc. to share information, seek advise, or share experiences. The Histonet is not a forum for blatant commercial advertising, but I am very impressed with this system and would recommend it to anyone who does frozen sections. The website is well put together and well worth investigating. (http://pathologyinnovations.com/) Paul Bradbury Canada Stephen Peters M.D. wrote: > For the last 5 years I have been promoting a system of face down embedding tissue > for frozen section which I developed over my 25 year surgical pathology practice. > The system is rapid, very precise and wastes very little tissue. I am all but exhausted devoting most of my vacation and free time to bring this sytem to my colleagues at very limited profit.My chucks are made y.to be used cold with deep wide > waffle pattern grooves. They are made of stainless so they function as a heat extractor. > With the exception of Leica, I am quite surprised and saddened by the lack of interest in > our cryostat manufacturers to explore a new technology which could benefit their customers. I believe they are hoping I fade away. If you want to improve your frozen section practice, I suggest you seek out this system. I would be happy to direct you to it, but the last time I offered help, one of the vendors complained I was breaking a vendor rule. > > Stephen > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From sccrshlly <@t> yahoo.com Thu Oct 11 20:49:38 2007 From: sccrshlly <@t> yahoo.com (Shelly Coker) Date: Fri Oct 12 01:33:49 2007 Subject: [Histonet] H&E Staining Message-ID: <255001.23376.qm@web90306.mail.mud.yahoo.com> If you are using an alcohol soluble eosin, I recommend removing the 70% alcohol from your procedure. Go straight to 95% after the eosin, then a couple changes of 100%. You can adjust the timing in the 95% alcohol to get it just right b/c the 95% alcohol does the differentiating of the eosin staining. The longer in the 95%, the less pink staining there will be. Alot of the timing depends on the type of eosin you are using. Happy staining! --------------------------------- Yahoo! oneSearch: Finally, mobile search that gives answers, not web links. From pieronelva01 <@t> bigpond.com Fri Oct 12 04:23:23 2007 From: pieronelva01 <@t> bigpond.com (Piero Nelva) Date: Fri Oct 12 04:23:33 2007 Subject: [Histonet] Cell block References: <2925AE271EAAD440AF48FCCEB8002D090542F837@smgmail01.smgj.sclhs.net> Message-ID: <005801c80cb1$8970e0c0$fc74be7c@pentium4> Hello - my first time on Histonet!! Our cyto lab picks out any formed clots and spins the rest into a cell block which is stabilised with plasma and thrombin. Processes and section well. Piero Nelva Anatomical Pathology Monash Medical Centre Melbourne Australia ----- Original Message ----- From: "Inman, Anna" To: Sent: Tuesday, October 09, 2007 12:47 AM Subject: [Histonet] Cell block Hello - For those you who perform cytology cell blocks - if the fluid (pleural, peritoneal, pericardial) has a "clot" (mucous, tumor or whatever) do you only submit the clot for cell block or do you also spin fluid down and add whatever button you may have, after decanting, to the clot for processing? Thank you Anna CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.5.488 / Virus Database: 269.14.5/1058 - Release Date: 10/8/2007 4:54 PM From dmccaig <@t> ckha.on.ca Fri Oct 12 10:24:33 2007 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Fri Oct 12 10:24:55 2007 Subject: [Histonet] Teratogens Message-ID: Hi I was wondering if anyone specifically labels these chemicals in your lab to alert any techs of child producing age. Also, how do you establish this criteria, some could be in the concentrated form or powder state, but do they still qualify in a dilute state? Is this determined strictly by the MSDS and based on the word teratogen or do you also consider terminology as mutagen, reproductive effector as well? If protective equipment and proper ventilation is available, are pregnant staff exempt for doing any activity or stain that uses these solutions for the full term of their pregnancy and "I want to become pregnant" stages? And finally, can another provide a list of the chemicals they class as teratogens. thanks Diana McCaig From jpiche-grocki <@t> wtbyhosp.org Fri Oct 12 11:33:10 2007 From: jpiche-grocki <@t> wtbyhosp.org (Piche-Grocki, Jessica) Date: Fri Oct 12 11:34:41 2007 Subject: [Histonet] Defrosting Cryostat Message-ID: <0A57D6AEAE4CBA4A984D27257160A72D01069CEE@win03exchange01.wtbyhosp.org> Hi, How often should we defrost our Cryostat? I have set it up for once a week. Should it be more often? Thanks, Jessica Piche-Grocki, HT(ASCP) CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From rjbuesa <@t> yahoo.com Fri Oct 12 12:23:30 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 12 12:25:16 2007 Subject: [Histonet] Teratogens In-Reply-To: Message-ID: <823783.2629.qm@web61214.mail.yahoo.com> Diana: 1- teratogen, mutagen or any such an indication in the MSDS should be considered as warnings for the label 2- at any concentration 3- all pregnant employees should (and deserve) being assigned low risk activities, even when 31% of USA histology labs do not practice this prohibition 4- you could Google "Teratogen and Mutagen" Ren? J. Diana McCaig wrote: Hi I was wondering if anyone specifically labels these chemicals in your lab to alert any techs of child producing age. Also, how do you establish this criteria, some could be in the concentrated form or powder state, but do they still qualify in a dilute state? Is this determined strictly by the MSDS and based on the word teratogen or do you also consider terminology as mutagen, reproductive effector as well? If protective equipment and proper ventilation is available, are pregnant staff exempt for doing any activity or stain that uses these solutions for the full term of their pregnancy and "I want to become pregnant" stages? And finally, can another provide a list of the chemicals they class as teratogens. thanks Diana McCaig _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better Heartthrob. Get better relationship answers from someone who knows. Yahoo! Answers - Check it out. From rjbuesa <@t> yahoo.com Fri Oct 12 12:24:59 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 12 12:25:18 2007 Subject: [Histonet] Defrosting Cryostat In-Reply-To: <0A57D6AEAE4CBA4A984D27257160A72D01069CEE@win03exchange01.wtbyhosp.org> Message-ID: <709197.83606.qm@web61222.mail.yahoo.com> Cryostats should be defrosted daily, near midnight. Ren? J. "Piche-Grocki, Jessica" wrote: Hi, How often should we defrost our Cryostat? I have set it up for once a week. Should it be more often? Thanks, Jessica Piche-Grocki, HT(ASCP) CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Building a website is a piece of cake. Yahoo! Small Business gives you all the tools to get online. From trinimaican2501 <@t> yahoo.com Fri Oct 12 13:04:36 2007 From: trinimaican2501 <@t> yahoo.com (I-sanna Gibbons) Date: Fri Oct 12 13:04:41 2007 Subject: [Histonet] proventricular dilatation images? Message-ID: <361313.20681.qm@web50310.mail.re2.yahoo.com> Hi all, Does anyone have histological images of proventricular dilatation and also images of the normal for comparison? Thanks I-sanna ____________________________________________________________________________________ Moody friends. Drama queens. Your life? Nope! - their life, your story. Play Sims Stories at Yahoo! Games. http://sims.yahoo.com/ From ccross6032 <@t> aol.com Fri Oct 12 13:27:07 2007 From: ccross6032 <@t> aol.com (Cheryl Cross) Date: Fri Oct 12 13:27:25 2007 Subject: [Histonet] proventricular dilatation images? In-Reply-To: <361313.20681.qm@web50310.mail.re2.yahoo.com> References: <361313.20681.qm@web50310.mail.re2.yahoo.com> Message-ID: <05462208-2622-43F5-95C0-52961EF6A3A0@aol.com> Hi I-sanna - I don't have any images readily available - there is a nice little review with some histopathology here, however: http://www.vet.uga.edu/vpp/ivcvm/1998/gregory/index.php What I can tell you is that in many cases can be a very very subtle disease - often there are just a few lymphocytes around the nerve ganglia - very very subtle. if you look at just a normal proventriculus histology book - an animal with really remarkable with have a thinned, stretched out proventriculus, but the histology can look very similar. It's easier to diagnose grossly (in my experience anyway), because the amount of inflammation histopathologically doesn't always match up with the degree of dilatation (ie animals with a marked dilation can have minimal inflammation). make sense? CC Cheryl Cross, DVM, Dipl. ACVP Researcher University Corporation for Atmospheric Research College of Veterinary Medicine University of Tennessee Department of Pathology 2407 River Drive, Room A201 Knoxville, TN 37996-4542 (423) 967-2724 fax: 865-974-5616 ccross@ucar.edu On Oct 12, 2007, at 2:04 PM, I-sanna Gibbons wrote: > Hi all, > > Does anyone have histological images of proventricular dilatation > and also images of the normal for comparison? > > Thanks > I-sanna > > > > ______________________________________________________________________ > ______________ > Moody friends. Drama queens. Your life? Nope! - their life, your > story. Play Sims Stories at Yahoo! Games. > http://sims.yahoo.com/ > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trinimaican2501 <@t> yahoo.com Fri Oct 12 14:21:39 2007 From: trinimaican2501 <@t> yahoo.com (I-sanna Gibbons) Date: Fri Oct 12 14:21:48 2007 Subject: [Histonet] proventricular dilatation images? Message-ID: <24335.52735.qm@web50301.mail.re2.yahoo.com> Cheryl Yes it does! Thanks. Will check the website I-sanna ----- Original Message ---- From: Cheryl Cross To: I-sanna Gibbons Cc: histonet@lists.utsouthwestern.edu Sent: Friday, October 12, 2007 3:27:07 PM Subject: Re: [Histonet] proventricular dilatation images? Hi I-sanna - I don't have any images readily available - there is a nice little review with some histopathology here, however: http://www.vet.uga.edu/vpp/ivcvm/1998/gregory/index.php What I can tell you is that in many cases can be a very very subtle disease - often there are just a few lymphocytes around the nerve ganglia - very very subtle. if you look at just a normal proventriculus histology book - an animal with really remarkable with have a thinned, stretched out proventriculus, but the histology can look very similar. It's easier to diagnose grossly (in my experience anyway), because the amount of inflammation histopathologically doesn't always match up with the degree of dilatation (ie animals with a marked dilation can have minimal inflammation). make sense? CC Cheryl Cross, DVM, Dipl. ACVP Researcher University Corporation for Atmospheric Research College of Veterinary Medicine University of Tennessee Department of Pathology 2407 River Drive, Room A201 Knoxville, TN 37996-4542 (423) 967-2724 fax: 865-974-5616 ccross@ucar.edu On Oct 12, 2007, at 2:04 PM, I-sanna Gibbons wrote: Hi all, Does anyone have histological images of proventricular dilatation and also images of the normal for comparison? Thanks I-sanna ____________________________________________________________________________________ Moody friends. Drama queens. Your life? Nope! - their life, your story. Play Sims Stories at Yahoo! Games. http://sims.yahoo.com/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet = ____________________________________________________________________________________ Check out the hottest 2008 models today at Yahoo! Autos. http://autos.yahoo.com/new_cars.html From jhabecke <@t> fhcrc.org Fri Oct 12 17:18:40 2007 From: jhabecke <@t> fhcrc.org (Randolph-Habecker, Julie) Date: Fri Oct 12 17:18:48 2007 Subject: [Histonet] Keeping frozen mouse aorta on slides Message-ID: <040346FA7309BD439C327F97D4C4D69B13F127@ISIS.fhcrc.org> Folks, I need your suggestions! I am working with an investigator who wants my lab to stain unfixed, OCT embedded mouse aortas. Unfortunately, they have already cut all of the tissue so we do not have a choice of slides. They were cut at 6 microns on Star frost slides and stored at -80C for a few months. When we received them, we took a few slides out, let them air dry at room temperature for 24 hours, put them in a slides rack, soaked them in room temperature PBS for 5 minutes and fixed them with 10% NBF for 10 minutes. These steps are done without agitation. This procedure is compatible with our staining protocol and normally works fine when we use Super frost or Gold Plus slides from Erie. However, in this case, the tissue is floating off the slides in both the PBS and the NBF stage. I have also tried to do these steps with the slides flat and get the same results. Any suggestion on ways to "glue" already cut frozen sections to a charged slide in ways that are compatible with x-gal and IHC staining? Thanks in advance for your help!!!! Julie Julie Randolph-Habecker, Ph.D. Staff Scientist - Director Experimental Histopathology Shared Resource Fred Hutchinson Cancer Research Center 1100 Fairview Ave, N. M5-A803 Seattle WA 98109-1024 206-667-6119 jhabecke@fhcrc.org From liz <@t> premierlab.com Fri Oct 12 17:28:14 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Oct 12 17:28:22 2007 Subject: [Histonet] polymer based IHC reagent for mouse antibodies on rat tissue Message-ID: Hello all Is anyone out there know of a polymer based reagent that will work for mouse antibodies on rat tissues? Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 From rjbuesa <@t> yahoo.com Sat Oct 13 08:46:17 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Oct 13 08:46:28 2007 Subject: [Histonet] Keeping frozen mouse aorta on slides In-Reply-To: <040346FA7309BD439C327F97D4C4D69B13F127@ISIS.fhcrc.org> Message-ID: <260570.30359.qm@web61212.mail.yahoo.com> Julie: The collagen nature of aortas make them more difficult to adhere. I think that you will need a "mechanical barrier" to restrain the sections. Try doing the whole procedure with the slides flat and the sections covered by a very fine plastic mesh that will hold the sections down by its weight. The mesh will allow all the reagents to act. The only problem will be when lifting the mesh that the sections may stick to it. The procedure could also be done through a piece of filter paper, but it is more likely to peel the sections than with the mesh. Just a thought, you may try 1 or 2 sections to see what happens. Ren? J. "Randolph-Habecker, Julie" wrote: Folks, I need your suggestions! I am working with an investigator who wants my lab to stain unfixed, OCT embedded mouse aortas. Unfortunately, they have already cut all of the tissue so we do not have a choice of slides. They were cut at 6 microns on Star frost slides and stored at -80C for a few months. When we received them, we took a few slides out, let them air dry at room temperature for 24 hours, put them in a slides rack, soaked them in room temperature PBS for 5 minutes and fixed them with 10% NBF for 10 minutes. These steps are done without agitation. This procedure is compatible with our staining protocol and normally works fine when we use Super frost or Gold Plus slides from Erie. However, in this case, the tissue is floating off the slides in both the PBS and the NBF stage. I have also tried to do these steps with the slides flat and get the same results. Any suggestion on ways to "glue" already cut frozen sections to a charged slide in ways that are compatible with x-gal and IHC staining? Thanks in advance for your help!!!! Julie Julie Randolph-Habecker, Ph.D. Staff Scientist - Director Experimental Histopathology Shared Resource Fred Hutchinson Cancer Research Center 1100 Fairview Ave, N. M5-A803 Seattle WA 98109-1024 206-667-6119 jhabecke@fhcrc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Check out the hottest 2008 models today at Yahoo! Autos. From contact <@t> excaliburpathology.com Sat Oct 13 09:14:36 2007 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Sat Oct 13 09:14:45 2007 Subject: [Histonet] Keeping frozen mouse aorta on slides Message-ID: <220369.5150.qm@web50102.mail.re2.yahoo.com> Try going right to formalin after air drying, instead of PBS. Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 630 N. Broadway Moore, OK 73160 405-570-6679 405-759-3953 contact@excaliburpathology.com From contact <@t> excaliburpathology.com Sat Oct 13 09:16:16 2007 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Sat Oct 13 09:16:23 2007 Subject: [Histonet] polymer based IHC reagent for mouse antibodies on rat tissue Message-ID: <122947.8101.qm@web50106.mail.re2.yahoo.com> Mouse ImmPress from Vector Labs. Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 630 N. Broadway Moore, OK 73160 405-570-6679 405-759-3953 contact@excaliburpathology.com From max_atena_parthenos <@t> alice.it Sat Oct 13 17:49:42 2007 From: max_atena_parthenos <@t> alice.it (max_atena_parthenos@alice.it) Date: Sat Oct 13 17:53:02 2007 Subject: [Histonet] Test Message-ID: This is only a test, please do not answer. Thank you From mariakatleba <@t> aol.com Sun Oct 14 20:19:37 2007 From: mariakatleba <@t> aol.com (mariakatleba@aol.com) Date: Sun Oct 14 20:21:15 2007 Subject: [Histonet] Jobs in Ireland Message-ID: <8C9DCE09612B69B-E8C-92D@WEBMAIL-MB10.sysops.aol.com> Anyone know how I can look for histology jobs in Ireland??? Thanks in advance Maria Katleba MS HT(ASCP) Napa California USA ________________________________________________________________________ Email and AIM finally together. You've gotta check out free AOL Mail! - http://mail.aol.com From atouvr <@t> yahoo.com Mon Oct 15 04:30:26 2007 From: atouvr <@t> yahoo.com (annamaria touvra) Date: Mon Oct 15 04:30:34 2007 Subject: [Histonet] Collecting PBMCs Message-ID: <840665.38722.qm@web60712.mail.yahoo.com> Goodmorning everyone, I would like some help concerning the collection/separation of Peripheral Mononuclear Cells (PBMCs) from whole blood. If anyone has done it with HIstopaque-1077 of Sigma-Aldrich, I would like to know which is the appropriate ratio of Histopaque and whole blood, at what temperature is the centrifugation done, how many rpm are used and for how long. The procedure mentions to use 15ml centrifuge tubes. What will happen if I'll use tubes of 5 or 10 ml? If there is anything else making the separation better I would appreciate any comment. Thank you in advance, Have a nice week full of joy in work, Anna-Maria --------------------------------- Pinpoint customers who are looking for what you sell. From relia1 <@t> earthlink.net Mon Oct 15 08:41:02 2007 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Oct 15 08:41:10 2007 Subject: [Histonet] Histo Tech and Cyto Tech needed in the San Francisco Bay Area. Can you help? Message-ID: Hi Histonetters, I hope you all had great weekends. I am presently on a search for one of my best clients that is in need of a Cyto Tech and a Histo Tech in the San Francisco Bay area. My client offers excellent compensation and relocation and offers an environment that is great to work in. The help I need is do you know anyone that might be interested in hearing about either of these opportunites? If so could you please forward my e-mail to them? If you are interested in the histo tech position or know a cyto tech that might be interested in the cytotechnologist position please call me ASAP at 866-607-3542 or e-mail me at relia1@earthlink.net Thank you, Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.myspace.com/pamatrelia From abright <@t> brightinstruments.com Mon Oct 15 09:37:13 2007 From: abright <@t> brightinstruments.com (Alan Bright) Date: Mon Oct 15 09:40:47 2007 Subject: [Histonet] CRYOSTAT TROUBLESHOOTING References: <585724.79940.qm@web45107.mail.sp1.yahoo.com> Message-ID: Dear Dr. Peters, There is no need to be saddened by the lack of interest of cryostat manufactures as we have a constant devotion to cryostat developments spanning over 50 years. We developed and made available for our cryostats over 14 years ago, 10 years ahead of you, a very superior system for face down tissue embedding, using frozen object holders and much faster freezing at -50 degs. C. I for one do not hope you fade away as we continually consult with users to establish new techniques and products to advance frozen sectioning. Perhaps when looking into future developments you would consider contacting us. Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com Skype: dazzle0 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephen Peters M.D. Sent: 11 October 2007 19:32 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CRYOSTAT TROUBLESHOOTING For the last 5 years I have been promoting a system of face down embedding tissue for frozen section which I developed over my 25 year surgical pathology practice. The system is rapid, very precise and wastes very little tissue. I am all but exhausted devoting most of my vacation and free time to bring this sytem to my colleagues at very limited profit.My chucks are made y.to be used cold with deep wide waffle pattern grooves. They are made of stainless so they function as a heat extractor. With the exception of Leica, I am quite surprised and saddened by the lack of interest in our cryostat manufacturers to explore a new technology which could benefit their customers. I believe they are hoping I fade away. If you want to improve your frozen section practice, I suggest you seek out this system. I would be happy to direct you to it, but the last time I offered help, one of the vendors complained I was breaking a vendor rule. Stephen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From torbertn <@t> wabash.edu Mon Oct 15 10:57:16 2007 From: torbertn <@t> wabash.edu (Neil Schmitzer-Torbert) Date: Mon Oct 15 10:58:58 2007 Subject: [Histonet] IEC manual and specimen holder Message-ID: <003501c80f44$0ecdcbe0$722b20a1@torbertnPC> Hello, I have recently come into possession of a IEC CTD cryostat with a minot microtome. I am hoping to find a copy of the manual for the cryostat and somewhere that I can purchase a specimen holder for the microtome. Any advice on where I can find these items would be greatly appreciated! Neil Schmitzer-Torbert BKT Assistant Professor of Psychology Wabash College Crawfordsville, IN 47933 (765) 361-6076 From ACortez <@t> hei.org Mon Oct 15 12:55:03 2007 From: ACortez <@t> hei.org (Cortez, Ana) Date: Mon Oct 15 12:55:10 2007 Subject: [Histonet] How do I prepare glycerin albumen. Message-ID: <87449E4A2B01DA47B29424CE5D6E0F83191744@hi0sml1.hei.org> Does anybody have a recipe for preparing glycerin albumen. I found a recipe online but it uses eggs. All I have is Egg albumen powder. Your help will be appreciated. Thanks Ana Cordero, HT(ASCP) Research Assistant technician House Ear Institute 2100 W. 3rd Street Los Angeles, CA 90057 From TJJ <@t> Stowers-Institute.org Mon Oct 15 13:12:18 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Mon Oct 15 13:12:35 2007 Subject: [Histonet] Re: Keeping frozen mouse aorta on slides Message-ID: Julie, you might try getting the Sequenza system, as it uses capillary gap action and the slide is mounted with the tissue against a face plate. What I would probably do is float them off and put them in multiwell plates, and do the staining free-floating. Then when you're finished, mount them on to slides, or visualize them in the well plate if you can. Good luck! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From rjbuesa <@t> yahoo.com Mon Oct 15 13:16:10 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Oct 15 13:16:15 2007 Subject: [Histonet] How do I prepare glycerin albumen. In-Reply-To: <87449E4A2B01DA47B29424CE5D6E0F83191744@hi0sml1.hei.org> Message-ID: <5652.48654.qm@web61212.mail.yahoo.com> Ana: The original glycerin albumen (by Mayer, 1880) calls for: 50 mL of fresh egg albumen + 50 mL of glycerol + 1 g of sodium salicilate. You have to agitate the mixture frequently for several days, and filter at the end. I don't know if the albumen powder will work, but I don't see any difficulty in getting fresh egg albumen. Filtration is very slow! Ren? J. "Cortez, Ana" wrote: Does anybody have a recipe for preparing glycerin albumen. I found a recipe online but it uses eggs. All I have is Egg albumen powder. Your help will be appreciated. Thanks Ana Cordero, HT(ASCP) Research Assistant technician House Ear Institute 2100 W. 3rd Street Los Angeles, CA 90057 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Moody friends. Drama queens. Your life? Nope! - their life, your story. Play Sims Stories at Yahoo! Games. From melissa.mazan <@t> tufts.edu Mon Oct 15 13:22:29 2007 From: melissa.mazan <@t> tufts.edu (Melissa Mazan) Date: Mon Oct 15 13:22:38 2007 Subject: [Histonet] anti-macrophage marker reactive to mouse In-Reply-To: <200710151714.l9FHEuUr016807@mail-proofpoint-2a.usg.tufts.edu> References: <200710151714.l9FHEuUr016807@mail-proofpoint-2a.usg.tufts.edu> Message-ID: <4713AFE5.1030306@tufts.edu> Hi all, I'm looking for an anti-mouse anti-macrophage marker that is NOT raised in mouse for use in immunohistochemistry (FFPE sections). Most of the antibodies that I've used in the past - CD45, Thy-1, CD11 - are too nonspecific being for anti-hematopoietic cells. Can anyone give a suggestion? Thanks - Melissa histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Jobs in Ireland (mariakatleba@aol.com) > 2. Collecting PBMCs (annamaria touvra) > 3. Histo Tech and Cyto Tech needed in the San Francisco Bay > Area. Can you help? (Pam Barker) > 4. RE: CRYOSTAT TROUBLESHOOTING (Alan Bright) > 5. IEC manual and specimen holder (Neil Schmitzer-Torbert) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sun, 14 Oct 2007 21:19:37 -0400 > From: mariakatleba@aol.com > Subject: [Histonet] Jobs in Ireland > To: histonet@lists.utsouthwestern.edu > Message-ID: <8C9DCE09612B69B-E8C-92D@WEBMAIL-MB10.sysops.aol.com> > Content-Type: text/plain; charset="us-ascii" > > Anyone know how I can look for histology jobs in Ireland??? > > Thanks in advance > > Maria Katleba MS HT(ASCP) > Napa California USA > ________________________________________________________________________ > Email and AIM finally together. You've gotta check out free AOL Mail! - http://mail.aol.com > > > ------------------------------ > > Message: 2 > Date: Mon, 15 Oct 2007 02:30:26 -0700 (PDT) > From: annamaria touvra > Subject: [Histonet] Collecting PBMCs > To: histonet@lists.utsouthwestern.edu > Message-ID: <840665.38722.qm@web60712.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > > Goodmorning everyone, > > I would like some help concerning the collection/separation of Peripheral Mononuclear Cells (PBMCs) from whole blood. > If anyone has done it with HIstopaque-1077 of Sigma-Aldrich, I would like to know which is the appropriate ratio of Histopaque and whole blood, at what temperature is the centrifugation done, how many rpm are used and for how long. The procedure mentions to use 15ml centrifuge tubes. What will happen if I'll use tubes of 5 or 10 ml? > If there is anything else making the separation better I would appreciate any comment. > > Thank you in advance, > > Have a nice week full of joy in work, > Anna-Maria > > > > --------------------------------- > Pinpoint customers who are looking for what you sell. > > ------------------------------ > > Message: 3 > Date: Mon, 15 Oct 2007 09:41:02 -0400 > From: "Pam Barker" > Subject: [Histonet] Histo Tech and Cyto Tech needed in the San > Francisco Bay Area. Can you help? > To: "'Histonet'" > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Hi Histonetters, > I hope you all had great weekends. I am presently on a search for one > of my best clients that is in need of a Cyto Tech and a Histo Tech in > the San Francisco Bay area. My client offers excellent compensation and > relocation and offers an environment that is great to work in. > > > > The help I need is do you know anyone that might be interested in > hearing about either of these opportunites? If so could you please > forward my e-mail to them? > > > > If you are interested in the histo tech position or know a cyto tech > that might be interested in the cytotechnologist position please call me > ASAP at 866-607-3542 or e-mail me at relia1@earthlink.net > > > > Thank you, > > > Thank You! > > > Pam Barker > President > RELIA > Specialists in Allied Healthcare Recruiting > 5703 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell: (407)353-5070 > FAX: (407)678-2788 > E-mail: relia1@earthlink.net > > www.myspace.com/pamatrelia > > > > ------------------------------ > > Message: 4 > Date: Mon, 15 Oct 2007 15:37:13 +0100 > From: "Alan Bright" > Subject: RE: [Histonet] CRYOSTAT TROUBLESHOOTING > To: "Stephen Peters M.D." , > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > > Dear Dr. Peters, > > There is no need to be saddened by the lack of interest of cryostat > manufactures as we have a constant devotion to cryostat developments > spanning over 50 years. > We developed and made available for our cryostats over 14 years ago, 10 > years ahead of you, a very superior system for face down tissue > embedding, using frozen object holders and much faster freezing at -50 > degs. C. > > I for one do not hope you fade away as we continually consult with users > to establish new techniques and products to advance frozen sectioning. > Perhaps when looking into future developments you would consider > contacting us. > > Best Regards > > Alan Bright > > Bright Instrument Co.Ltd. > St Margaret's Way > Huntingdon > Cambridgeshire > PE29 6EU > England > > Tel No:+44 (0)1480 454528 > Fax No:+44 (0)1480 456031 > Email: abright@brightinstruments.com > Web Site: www.brightinstruments.com > Skype: dazzle0 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephen > Peters M.D. > Sent: 11 October 2007 19:32 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] CRYOSTAT TROUBLESHOOTING > > For the last 5 years I have been promoting a system of face down > embedding tissue > for frozen section which I developed over my 25 year surgical > pathology practice. > The system is rapid, very precise and wastes very little tissue. I am > all but exhausted devoting most of my vacation and free time to bring > this sytem to my colleagues at very limited profit.My chucks are made > y.to be used cold with deep wide > waffle pattern grooves. They are made of stainless so they function > as a heat extractor. > With the exception of Leica, I am quite surprised and saddened by the > lack of interest in > our cryostat manufacturers to explore a new technology which could > benefit their customers. I believe they are hoping I fade away. If you > want to improve your frozen section practice, I suggest you seek out > this system. I would be happy to direct you to it, but the last time I > offered help, one of the vendors complained I was breaking a vendor > rule. > > Stephen > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 5 > Date: Mon, 15 Oct 2007 11:57:16 -0400 > From: "Neil Schmitzer-Torbert" > Subject: [Histonet] IEC manual and specimen holder > To: > Message-ID: <003501c80f44$0ecdcbe0$722b20a1@torbertnPC> > Content-Type: text/plain; charset="US-ASCII" > > Hello, > > > > I have recently come into possession of a IEC CTD cryostat with a minot > microtome. I am hoping to find a copy of the manual for the cryostat and > somewhere that I can purchase a specimen holder for the microtome. Any > advice on where I can find these items would be greatly appreciated! > > > > Neil Schmitzer-Torbert > > > > BKT Assistant Professor of Psychology > > Wabash College > > Crawfordsville, IN 47933 > > (765) 361-6076 > > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 47, Issue 17 > **************************************** > From liz <@t> premierlab.com Mon Oct 15 13:55:25 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Mon Oct 15 13:55:34 2007 Subject: [Histonet] anti-macrophage marker reactive to mouse In-Reply-To: <49ABE08F66CB484D8E045BB292437911@PremierLab.local> References: <200710151714.l9FHEuUr016807@mail-proofpoint-2a.usg.tufts.edu> <49ABE08F66CB484D8E045BB292437911@PremierLab.local> Message-ID: You can use either F4/80 or CD68 from serotec. Both work in FFPE tissues. They are rat anti-mouse antibodies. I have protocols if you need them. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Melissa Mazan Sent: Monday, October 15, 2007 12:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] anti-macrophage marker reactive to mouse Hi all, I'm looking for an anti-mouse anti-macrophage marker that is NOT raised in mouse for use in immunohistochemistry (FFPE sections). Most of the antibodies that I've used in the past - CD45, Thy-1, CD11 - are too nonspecific being for anti-hematopoietic cells. Can anyone give a suggestion? Thanks - Melissa histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Jobs in Ireland (mariakatleba@aol.com) > 2. Collecting PBMCs (annamaria touvra) > 3. Histo Tech and Cyto Tech needed in the San Francisco Bay > Area. Can you help? (Pam Barker) > 4. RE: CRYOSTAT TROUBLESHOOTING (Alan Bright) > 5. IEC manual and specimen holder (Neil Schmitzer-Torbert) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sun, 14 Oct 2007 21:19:37 -0400 > From: mariakatleba@aol.com > Subject: [Histonet] Jobs in Ireland > To: histonet@lists.utsouthwestern.edu > Message-ID: <8C9DCE09612B69B-E8C-92D@WEBMAIL-MB10.sysops.aol.com> > Content-Type: text/plain; charset="us-ascii" > > Anyone know how I can look for histology jobs in Ireland??? > > Thanks in advance > > Maria Katleba MS HT(ASCP) > Napa California USA > ______________________________________________________________________ > __ Email and AIM finally together. You've gotta check out free AOL > Mail! - http://mail.aol.com > > > ------------------------------ > > Message: 2 > Date: Mon, 15 Oct 2007 02:30:26 -0700 (PDT) > From: annamaria touvra > Subject: [Histonet] Collecting PBMCs > To: histonet@lists.utsouthwestern.edu > Message-ID: <840665.38722.qm@web60712.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > > Goodmorning everyone, > > I would like some help concerning the collection/separation of Peripheral Mononuclear Cells (PBMCs) from whole blood. > If anyone has done it with HIstopaque-1077 of Sigma-Aldrich, I would like to know which is the appropriate ratio of Histopaque and whole blood, at what temperature is the centrifugation done, how many rpm are used and for how long. The procedure mentions to use 15ml centrifuge tubes. What will happen if I'll use tubes of 5 or 10 ml? > If there is anything else making the separation better I would appreciate any comment. > > Thank you in advance, > > Have a nice week full of joy in work, > Anna-Maria > > > > --------------------------------- > Pinpoint customers who are looking for what you sell. > > ------------------------------ > > Message: 3 > Date: Mon, 15 Oct 2007 09:41:02 -0400 > From: "Pam Barker" > Subject: [Histonet] Histo Tech and Cyto Tech needed in the San > Francisco Bay Area. Can you help? > To: "'Histonet'" > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Hi Histonetters, > I hope you all had great weekends. I am presently on a search for one > of my best clients that is in need of a Cyto Tech and a Histo Tech in > the San Francisco Bay area. My client offers excellent compensation > and relocation and offers an environment that is great to work in. > > > > The help I need is do you know anyone that might be interested in > hearing about either of these opportunites? If so could you please > forward my e-mail to them? > > > > If you are interested in the histo tech position or know a cyto tech > that might be interested in the cytotechnologist position please call > me ASAP at 866-607-3542 or e-mail me at relia1@earthlink.net > > > > Thank you, > > > Thank You! > > > Pam Barker > President > RELIA > Specialists in Allied Healthcare Recruiting > 5703 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell: (407)353-5070 > FAX: (407)678-2788 > E-mail: relia1@earthlink.net > > www.myspace.com/pamatrelia > > > > ------------------------------ > > Message: 4 > Date: Mon, 15 Oct 2007 15:37:13 +0100 > From: "Alan Bright" > Subject: RE: [Histonet] CRYOSTAT TROUBLESHOOTING > To: "Stephen Peters M.D." , > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > > Dear Dr. Peters, > > There is no need to be saddened by the lack of interest of cryostat > manufactures as we have a constant devotion to cryostat developments > spanning over 50 years. > We developed and made available for our cryostats over 14 years ago, > 10 years ahead of you, a very superior system for face down tissue > embedding, using frozen object holders and much faster freezing at -50 > degs. C. > > I for one do not hope you fade away as we continually consult with > users to establish new techniques and products to advance frozen sectioning. > Perhaps when looking into future developments you would consider > contacting us. > > Best Regards > > Alan Bright > > Bright Instrument Co.Ltd. > St Margaret's Way > Huntingdon > Cambridgeshire > PE29 6EU > England > > Tel No:+44 (0)1480 454528 > Fax No:+44 (0)1480 456031 > Email: abright@brightinstruments.com > Web Site: www.brightinstruments.com > Skype: dazzle0 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Stephen Peters M.D. > Sent: 11 October 2007 19:32 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] CRYOSTAT TROUBLESHOOTING > > For the last 5 years I have been promoting a system of face down > embedding tissue > for frozen section which I developed over my 25 year surgical > pathology practice. > The system is rapid, very precise and wastes very little tissue. I > am all but exhausted devoting most of my vacation and free time to > bring this sytem to my colleagues at very limited profit.My chucks are > made y.to be used cold with deep wide > waffle pattern grooves. They are made of stainless so they function > as a heat extractor. > With the exception of Leica, I am quite surprised and saddened by > the lack of interest in > our cryostat manufacturers to explore a new technology which could > benefit their customers. I believe they are hoping I fade away. If you > want to improve your frozen section practice, I suggest you seek out > this system. I would be happy to direct you to it, but the last time I > offered help, one of the vendors complained I was breaking a vendor > rule. > > Stephen > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 5 > Date: Mon, 15 Oct 2007 11:57:16 -0400 > From: "Neil Schmitzer-Torbert" > Subject: [Histonet] IEC manual and specimen holder > To: > Message-ID: <003501c80f44$0ecdcbe0$722b20a1@torbertnPC> > Content-Type: text/plain; charset="US-ASCII" > > Hello, > > > > I have recently come into possession of a IEC CTD cryostat with a > minot microtome. I am hoping to find a copy of the manual for the > cryostat and somewhere that I can purchase a specimen holder for the > microtome. Any advice on where I can find these items would be greatly appreciated! > > > > Neil Schmitzer-Torbert > > > > BKT Assistant Professor of Psychology > > Wabash College > > Crawfordsville, IN 47933 > > (765) 361-6076 > > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 47, Issue 17 > **************************************** > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bendahhou <@t> ipmc.cnrs.fr Mon Oct 15 15:14:19 2007 From: bendahhou <@t> ipmc.cnrs.fr (Said Bendahhou) Date: Mon Oct 15 15:14:47 2007 Subject: [Histonet] Staining Muscle proteins Message-ID: <361578D5-1CC7-432B-957C-D6502B3580C3@ipmc.cnrs.fr> Hi all, I'm looking for a protocol to label skeletal muscle compartments (T- tubules, SR...) of frozen sections, using mice antibodies. Mice muscle was cut and embedded in OCT, frozen using isopentane, and cut at 8 um. Cover slips were stored at -20C. Expecting details regarding fixation, permeabilization for better access to all compartments. Secondary antibodies used are Alexa 488 or 594. Thanks, Said From BLORD <@t> swmail.sw.org Mon Oct 15 15:30:50 2007 From: BLORD <@t> swmail.sw.org (Barbara Lord) Date: Mon Oct 15 15:31:08 2007 Subject: [Histonet] Freezing specimens for frozens Message-ID: I agree with everything that Dawn said about Dr. Peters system. I have been using it for about 4 years and just love it. I have been cutting frozens for about 35 years and this is the easiest and most reliable method for freezing specimens, flat surface etc.... Dr. Peters has done a great job bringing his method to the rest of histo world and deserves a great THANK YOU. I have been cutting Mohs for 5 years and of all the ways that people have come up with to get a flat surface of skin for Mohs, this is hands down the only way in my book. All have a great day Barbara Scott & White Hospital Dept of Dermatology Temple, TX From ACortez <@t> hei.org Mon Oct 15 15:35:54 2007 From: ACortez <@t> hei.org (Cortez, Ana) Date: Mon Oct 15 15:36:00 2007 Subject: [Histonet] Thank you Message-ID: <87449E4A2B01DA47B29424CE5D6E0F83191747@hi0sml1.hei.org> Thanks to those who reply to my message. From Eric.Hoy <@t> UTSouthwestern.edu Mon Oct 15 17:38:29 2007 From: Eric.Hoy <@t> UTSouthwestern.edu (Eric Hoy) Date: Mon Oct 15 17:38:35 2007 Subject: [Histonet] Re: Histonet Digest, Vol 47, Issue 17 In-Reply-To: <200710151658.l9FGwY7U006177@nlpi003.prodigy.net> Message-ID: Anna-Maria asked: > I would like some help concerning the collection/separation of Peripheral > Mononuclear Cells (PBMCs) from whole blood. > If anyone has done it with HIstopaque-1077 of Sigma-Aldrich, I would like to > know which is the appropriate ratio of Histopaque and whole blood, at what > temperature is the centrifugation done, how many rpm are used and for how > long. The procedure mentions to use 15ml centrifuge tubes. What will happen if > I'll use tubes of 5 or 10 ml? > If there is anything else making the separation better I would appreciate > any comment. > > Thank you in advance, > > Have a nice week full of joy in work, > Anna-Maria The method I have used for flow cytometry is as follows: 1) Pipet 3 ml of anticoagulated blood into a 13 x 100 tube. 2) Dilute blood 1:2 with 3 ml of PBS. Invert gently to mix. 3) Pipet 5 ml of Histopaque-1077 into a 15 ml conical centrifuge tube. 4) Carefully overlay the entire diluted blood sample onto the Histopaque. Do not allow the blood and Histopaque to mix. (One tech with whom I worked would put the diluted blood in the centrifuge tube first, then use a long-tipped Pasteur pipet to introduce the Histopaque under the blood sample. I found that technique more difficult, and could never demonstrate a difference.) 4) Cap the tube and centrifuge 25 minutes at 1200 rpm. (This is in a Silencer 2110 centrifuge with a swinging-bucket rotor. The RCF is about 200-220 G. Slow and gentle.) The centrifugation is at room temperature. After spinning you will have four layers: plasma on top, PBMC, histopaque, and RBC/granulocytes. We remove the plasma with a pipet, and then harvest the PBMC. Good luck with your cell separations! Eric =================================================== Eric S. Hoy, Ph.D., SI(ASCP) Clinical Associate Professor Department of Medical Laboratory Sciences The University of Texas Southwestern Medical Center Dallas, Texas Email: Eric.Hoy@UTSouthwestern.edu =================================================== From max_atena_parthenos <@t> alice.it Mon Oct 15 17:38:03 2007 From: max_atena_parthenos <@t> alice.it (max_atena_parthenos@alice.it) Date: Mon Oct 15 17:40:22 2007 Subject: [Histonet] Test - do not answer please Message-ID: test From histology.bc <@t> shaw.ca Mon Oct 15 18:48:13 2007 From: histology.bc <@t> shaw.ca (Paul Bradbury) Date: Mon Oct 15 18:49:20 2007 Subject: [Histonet] How do I prepare glycerin albumen. In-Reply-To: <87449E4A2B01DA47B29424CE5D6E0F83191744@hi0sml1.hei.org> References: <87449E4A2B01DA47B29424CE5D6E0F83191744@hi0sml1.hei.org> Message-ID: <4713FC3D.3070004@shaw.ca> Just use an egg as the recipe suggests. Separate the yolk and the white ... and there you are. Don't over-complicate things. Paul Cortez, Ana wrote: > Does anybody have a recipe for preparing glycerin albumen. I found a recipe online but it uses eggs. > All I have is Egg albumen powder. Your help will be appreciated. > > Thanks > > Ana Cordero, HT(ASCP) > Research Assistant technician > House Ear Institute > 2100 W. 3rd Street > Los Angeles, CA 90057 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From Kemlo.Rogerson <@t> waht.swest.nhs.uk Tue Oct 16 02:12:19 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Oct 16 02:12:26 2007 Subject: [Histonet] How do I prepare glycerin albumen. Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222EF40@wahtntex2.waht.swest.nhs.uk> If you fried that would it be an aspirin omelette? Sounds rather appetising. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Biology is the least of what makes someone a mother. --Oprah Winfrey This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Kemlo.Rogerson <@t> waht.swest.nhs.uk Tue Oct 16 02:13:04 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Oct 16 02:13:12 2007 Subject: [Histonet] How do I prepare glycerin albumen. Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222EF41@wahtntex2.waht.swest.nhs.uk> I assumed they didn't have chickens in LA. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Biology is the least of what makes someone a mother. --Oprah Winfrey This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From sbreeden <@t> nmda.nmsu.edu Tue Oct 16 07:04:10 2007 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Oct 16 07:04:17 2007 Subject: [Histonet] AutoStainer XL Tweaking Message-ID: <4D14F0FC9316DD41972D5F03C070908B8F46DA@nmdamailsvr.nmda.ad.nmsu.edu> I'm setting up my Leica AutoStainer XL and would appreciate any help with tweaking the times. I based my timing for the Autostainer on my manual stain times but staining is not crisp and looks washed out. Docs say eosin is fine but cellular detail is foggy. Here are my times: XYL - 3 at 5 min each 100% reagent - 2 at 30 seconds each 95% reagent - 1 at 30 seconds 80% reagent - 1 at 30 seconds DI water wash - 1 minute Hematox (currently using Sigma) - 5 minutes DI water wash - 1 minute Acid Alcohol (1%) - one dip DI water wash - 1 minute Ammonia water - 15 seconds DI water wash - 1 minute 80% reagent - 1 at 30 seconds 95% reagent - 1 at 30 seconds Eosin (alcoholic) - 10 seconds 95% reagent - 1 at 30 seconds 100% reagent - 2 at 1 minute each XYL - 3 at 1 minute each I cut my acid alcohol time down from 15 seconds to one dip but it's still taking a lot of hematoxylin off. Should I try 0.5% acid alcohol? I'm at a loss so far; different hematox? More time in ammonia water? If you use this Leica AutoStainer XL, could you offer some Handy Hints? I thank you SO much! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From gvdobbin <@t> ihis.org Tue Oct 16 07:15:52 2007 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Tue Oct 16 07:16:08 2007 Subject: [Histonet] Washed out nuclei resolved Message-ID: For the benefit of anyone who read my original post and were awaiting the outcome, the following explanation was arrived at after investigating what may have been done differently in the lead up to the the problem and with the help and advise of many of you, my Histonet colleagues! Thank you. I think what has happened is that our processor normally gets all of its reagents changed on Mondays. This past Monday was Canadian Thanksgiving, a holiday. Then on Tuesday and Wednesday mornings our Assistant was tied up with autopsies and so the processor did not get its reagents changed until Thursday. And as it happens we had unually high workloads on both Thursday night last week and Wednesday night this week (the night we processed the offending specimens). Not all of the cassettes were affected equally, but my best guess is that those with biopsy pads (and not all with biopsy pads mind you) were somehow more susceptible to the poor reagents than the non-biopsy pad specimens-perhaps because of increased carryover in the pads and as was suggested, by some, not all tissues are "created equal" (ie different consistencies, densities, etc.). Thanks again to all to took time to respond! Greg Original post: Hi Folks, Mystery to solve. Some of the surgical cases that came off our stainer today had very pale washed out looking nuclei. Our H&E control was fine (ran it twice) so it tells me the problem is either at the source or in the tissue processor. However, since not all cases are affected, I can't see how it could be the processor (VIP 5). So if it is the source, what are we talking about-something other than formalin in their formalin bottles? Contaminated formalin? Insufficient fixation time? Any and all comments will be welcome. Thanks in advance (again!) Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 There is some merit in doing the right thing rather badly, but absolutely none in doing the wrong thing excellently! Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ayant pour objet une personne ou un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer compl?tement de votre syst?me informatique. From jqb7 <@t> CDC.GOV Tue Oct 16 07:28:45 2007 From: jqb7 <@t> CDC.GOV (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Tue Oct 16 07:29:03 2007 Subject: [Histonet] AutoStainer XL Tweaking In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B8F46DA@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B8F46DA@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <34BB307EFC9A65429BBB49E330675F72045E2061@LTA3VS003.ees.hhs.gov> Which type of hematoxylin, etc. are you using? (I know you said Sigma, but do they have more than one formula?) Are you using their acid alcohol or are you making your own? Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Tuesday, October 16, 2007 8:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AutoStainer XL Tweaking I'm setting up my Leica AutoStainer XL and would appreciate any help with tweaking the times. I based my timing for the Autostainer on my manual stain times but staining is not crisp and looks washed out. Docs say eosin is fine but cellular detail is foggy. Here are my times: XYL - 3 at 5 min each 100% reagent - 2 at 30 seconds each 95% reagent - 1 at 30 seconds 80% reagent - 1 at 30 seconds DI water wash - 1 minute Hematox (currently using Sigma) - 5 minutes DI water wash - 1 minute Acid Alcohol (1%) - one dip DI water wash - 1 minute Ammonia water - 15 seconds DI water wash - 1 minute 80% reagent - 1 at 30 seconds 95% reagent - 1 at 30 seconds Eosin (alcoholic) - 10 seconds 95% reagent - 1 at 30 seconds 100% reagent - 2 at 1 minute each XYL - 3 at 1 minute each I cut my acid alcohol time down from 15 seconds to one dip but it's still taking a lot of hematoxylin off. Should I try 0.5% acid alcohol? I'm at a loss so far; different hematox? More time in ammonia water? If you use this Leica AutoStainer XL, could you offer some Handy Hints? I thank you SO much! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Tue Oct 16 08:27:11 2007 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Oct 16 08:24:35 2007 Subject: [Histonet] AutoStainer XL Tweaking In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B8F46DA@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B8F46DA@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <1F937FB30BDB7C4A9F39F83FEA8D379F3E4C96@bruexchange1.digestivespecialists.com> Hi Sara, Here is my schedule for H&E. It's not the Leica stainer but Microm DS50. Maybe this will help. Baseball in Denver at the NSH?? Linda 1 Oven 15 minutes 2 Xylene 3 minutes 3 Xylene 3 minutes 4 Xylene 3 minutes 5 100 % Alcohol 1 minute 6 100 % Alcohol 1 minute 7 100 % Alcohol 1 minute 8 95% Alcohol 1 minute 9 95% Alcohol 1 minute 10 Running Water 2 minutes 11 Hematoxylin 3 minutes 12 Running Water 2 minutes 13 5% acetic acid 15 seconds 14 Running Water 1 minute 15 Bluing Reagent 1 minute 16 Running Water 1 minute 17 95% Alcohol 15 seconds 18 Eosin 2 minutes 19 100% Alcohol 15 seconds 20 100 % Alcohol 1 minute 21 100 % Alcohol 1 minute 22 100 % Alcohol 1 minute 23 Xylene 2 minute 24 Xylene 2 minute 25 Xylene 2 minute Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Tuesday, October 16, 2007 8:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AutoStainer XL Tweaking I'm setting up my Leica AutoStainer XL and would appreciate any help with tweaking the times. I based my timing for the Autostainer on my manual stain times but staining is not crisp and looks washed out. Docs say eosin is fine but cellular detail is foggy. Here are my times: XYL - 3 at 5 min each 100% reagent - 2 at 30 seconds each 95% reagent - 1 at 30 seconds 80% reagent - 1 at 30 seconds DI water wash - 1 minute Hematox (currently using Sigma) - 5 minutes DI water wash - 1 minute Acid Alcohol (1%) - one dip DI water wash - 1 minute Ammonia water - 15 seconds DI water wash - 1 minute 80% reagent - 1 at 30 seconds 95% reagent - 1 at 30 seconds Eosin (alcoholic) - 10 seconds 95% reagent - 1 at 30 seconds 100% reagent - 2 at 1 minute each XYL - 3 at 1 minute each I cut my acid alcohol time down from 15 seconds to one dip but it's still taking a lot of hematoxylin off. Should I try 0.5% acid alcohol? I'm at a loss so far; different hematox? More time in ammonia water? If you use this Leica AutoStainer XL, could you offer some Handy Hints? I thank you SO much! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mauger <@t> email.chop.edu Tue Oct 16 09:00:41 2007 From: mauger <@t> email.chop.edu (Joanne Mauger) Date: Tue Oct 16 09:01:20 2007 Subject: [Histonet] protocol mounting media-xylene Message-ID: Hi Histotechs, Anyone having trouble with this mounting media being thinner than usual? We are having a lot of trouble with our coverslipper- Hacker robot, glass cover slips. We have always used this mountant, and the problems have been very recent- cover slips popping off after drying as if they didn't get anything but xylene! Coverslipper has been checked out as OK. Anyone get a bad batch? Company isn't aware of problems. I have lot 75461, exp 4/09 Thanks, Jo Mauger From Janet.Bonner <@t> FLHOSP.ORG Tue Oct 16 09:21:46 2007 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Tue Oct 16 09:22:38 2007 Subject: FW: [Histonet] AutoStainer XL Tweaking References: <4D14F0FC9316DD41972D5F03C070908B8F46DA@nmdamailsvr.nmda.ad.nmsu.edu> <5F31F38C96781A4FBE3196EBC22D47802E116E@fhosxchmb006.ADVENTISTCORP.NET> Message-ID: <5F31F38C96781A4FBE3196EBC22D47802E116F@fhosxchmb006.ADVENTISTCORP.NET> Sara, Here is our procedure, years of tweaking (it seems) every time the weather changed! Good Luck! Janet Florida Hospital Orlando ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Breeden, Sara Sent: Tue 10/16/2007 8:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AutoStainer XL Tweaking I'm setting up my Leica AutoStainer XL and would appreciate any help with tweaking the times. I based my timing for the Autostainer on my manual stain times but staining is not crisp and looks washed out. Docs say eosin is fine but cellular detail is foggy. Here are my times: YOURS OURS XYL - 3 at 5 min each ...................................................... 3 at 3 minutes 100% reagent - 2 at 30 seconds each ................................ 2 at 30 seconds each 95% reagent - 1 at 30 seconds ........................................... 1 at 30 seconds 80% reagent - 1 at 30 seconds ................................................................. none DI water wash - 1 minute ......................................................tap water (connected to faucet) - 1 minute Hematox (currently using Sigma) - 5 minutes ............... hematox (Richard Allen #7211) - 2 minutes Hematox #2 ........................................................................... hematox (R-A #7211) - 3 minutes DI water wash - 1 minute ....................................................... tap water - 1 minute Acid Alcohol (1%) - one dip ................................. 1% acid alcohol (bought from Polysciences for control issues)-5 seconds DI water wash - 1 minute ..................................................... tap water - 1 minute Ammonia water - 15 seconds .................................................Richard-Allen Bluing Solution -1 minute DI water wash - 1 minute ...........................................................tap water - 1 minute 80% reagent - 1 at 30 seconds ....................................................... none 95% reagent - 1 at 30 seconds ................................................. 1 at 30 seconds Eosin (alcoholic) - 10 seconds .............................................. Richard-Allen Eosin #7111(1:1 with 95% EtOH) - 15 seconds 95% reagent - 1 at 30 seconds ................................................. 1 at 30 seconds 100% reagent - 2 at 1 minute each ......................................... 2 at 1 minute each XYL - 3 at 1 minute each ...................................................... 1 at 1 minute Xylene ........................................................................ 2 at 2:30 minutes I cut my acid alcohol time down from 15 seconds to one dip but it's still taking a lot of hematoxylin off. Should I try 0.5% acid alcohol? I'm at a loss so far; different hematox? More time in ammonia water? If you use this Leica AutoStainer XL, could you offer some Handy Hints? I thank you SO much! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From mari.ann.mailhiot <@t> leica-microsystems.com Tue Oct 16 09:27:34 2007 From: mari.ann.mailhiot <@t> leica-microsystems.com (mari.ann.mailhiot@leica-microsystems.com) Date: Tue Oct 16 09:27:47 2007 Subject: [Histonet] AutoStainer XL Tweaking In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B8F46DA@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: Hi Sally I will give you a call. Best Regards Mari Ann Mailhiot BA HT ASCP Application Specialist Leica Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com "Breeden, Sara" To Sent by: histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] AutoStainer XL Tweaking 10/16/2007 07:04 AM I'm setting up my Leica AutoStainer XL and would appreciate any help with tweaking the times. I based my timing for the Autostainer on my manual stain times but staining is not crisp and looks washed out. Docs say eosin is fine but cellular detail is foggy. Here are my times: XYL - 3 at 5 min each 100% reagent - 2 at 30 seconds each 95% reagent - 1 at 30 seconds 80% reagent - 1 at 30 seconds DI water wash - 1 minute Hematox (currently using Sigma) - 5 minutes DI water wash - 1 minute Acid Alcohol (1%) - one dip DI water wash - 1 minute Ammonia water - 15 seconds DI water wash - 1 minute 80% reagent - 1 at 30 seconds 95% reagent - 1 at 30 seconds Eosin (alcoholic) - 10 seconds 95% reagent - 1 at 30 seconds 100% reagent - 2 at 1 minute each XYL - 3 at 1 minute each I cut my acid alcohol time down from 15 seconds to one dip but it's still taking a lot of hematoxylin off. Should I try 0.5% acid alcohol? I'm at a loss so far; different hematox? More time in ammonia water? If you use this Leica AutoStainer XL, could you offer some Handy Hints? I thank you SO much! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From melissa.farmer <@t> mail.mcgill.ca Tue Oct 16 09:46:08 2007 From: melissa.farmer <@t> mail.mcgill.ca (Melissa Farmer) Date: Tue Oct 16 09:47:38 2007 Subject: [Histonet] multiplex cytometry of mouse vaginal fluid for cytokine analysis Message-ID: I am desparately seeking information about whether anyone has successfully detected cytokines (TNFa, IFNy, IL-1, IL-4, IL-6) in mouse vaginal fluid using multiplex assays (specifically with the Luminex 100 system). I fear that cytokine levels may not be detectable, and the literature using this technique is quite limited regardling rodent vaginal fluid. Any wisdom? A million thanks-- Melissa Farmer Department of Psychology McGill University From sheila_adey <@t> hotmail.com Tue Oct 16 10:54:08 2007 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Tue Oct 16 10:54:17 2007 Subject: [Histonet] AutoStainer XL Tweaking In-Reply-To: References: <4D14F0FC9316DD41972D5F03C070908B8F46DA@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: Hi Sally, We use our Hematoxylin progressively and have no problems. Here is our protocol, hope it helps. We also use the Leica XL. You are right, I think the one dip would still be along time because of the speed of the dip. Station Oven 12 min #1 Xylene 3 min #2 Xylene 3 min #3 100% 30 sec #4 100% 30 sec #5 95% 20 sec #6 70% 20 sec W1 H20 20 sec #7 Gills 55 sec III Hematoxylin (surgipath) W2 H20 20 sec #11 Clarifier 20 sec Richard Allen W3 H20 30 sec #12 Bluing 40 sec Richard Allen W4 H20 30 sec #13 Eosin 20 sec Surgipath #14 95% 20 sec #15 100% 20 sec #16 100% 20 sec #17 Xylene 10 sec #18 Xylene 10 sec #19 Xylene Exit Note that if we are bringing IHCs to water we increase the Xylene times to 5min each and the Alcohols to 2 min each. Sheila Adey HT MLTPort Huron HospitalMichigan> To: sbreeden@nmda.nmsu.edu> From: mari.ann.mailhiot@leica-microsystems.com> Date: Tue, 16 Oct 2007 09:27:34 -0500> Subject: Re: [Histonet] AutoStainer XL Tweaking> CC: histonet@lists.utsouthwestern.edu> > Hi Sally> > I will give you a call.> > Best Regards> > Mari Ann Mailhiot BA HT ASCP> Application Specialist> Leica Technical Assistance Center> 800 248 0123 x7267> 847 236 3063 fax> mari.ann.mailhiot@leica-microsystems.com> www.leica-microsystems.com> > > > "Breeden, Sara" > su.edu> To > Sent by: > histonet-bounces@ cc > lists.utsouthwest > ern.edu Subject > [Histonet] AutoStainer XL Tweaking > > 10/16/2007 07:04 > AM > > > > > > > I'm setting up my Leica AutoStainer XL and would appreciate any help> with tweaking the times. I based my timing for the Autostainer on my> manual stain times but staining is not crisp and looks washed out. Docs> say eosin is fine but cellular detail is foggy. Here are my times:> > XYL - 3 at 5 min each> > 100% reagent - 2 at 30 seconds each> > 95% reagent - 1 at 30 seconds> > 80% reagent - 1 at 30 seconds> > DI water wash - 1 minute> > Hematox (currently using Sigma) - 5 minutes> > DI water wash - 1 minute> > Acid Alcohol (1%) - one dip> > DI water wash - 1 minute> > Ammonia water - 15 seconds> > DI water wash - 1 minute> > 80% reagent - 1 at 30 seconds> > 95% reagent - 1 at 30 seconds> > Eosin (alcoholic) - 10 seconds> > 95% reagent - 1 at 30 seconds> > 100% reagent - 2 at 1 minute each> > XYL - 3 at 1 minute each> > I cut my acid alcohol time down from 15 seconds to one dip but it's> still taking a lot of hematoxylin off. Should I try 0.5% acid alcohol?> I'm at a loss so far; different hematox? More time in ammonia water? If> you use this Leica AutoStainer XL, could you offer some Handy Hints? I> thank you SO much!> > > > Sally Breeden, HT(ASCP)> > NM Dept. of Agriculture> > Veterinary Diagnostic Services> > PO Box 4700> > Albuquerque, NM 87106> > 505-841-2576> > > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > ______________________________________________________________________> This email has been scanned by the MessageLabs Email Security System.> For more information please visit http://www.messagelabs.com/email > ______________________________________________________________________> > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Have fun while connecting on Messenger! Click here to learn more. http://entertainment.sympatico.msn.ca/WindowsLiveMessenger From Bauer.Karen <@t> mayo.edu Tue Oct 16 12:44:37 2007 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen) Date: Tue Oct 16 12:45:14 2007 Subject: [Histonet] Herpes Simplex Virus Controls Message-ID: Hello to all... It's a cold, dark, and dreary day in Wisconsin and my husband actually saw snow flakes last week! I'm dreading the winter weather as we approach the holidays. Oh well, on to business... We are in desperate need of some HSV controls and I'm hoping someone out there has a few I could have. I would be willing to swap with some controls that we have in abundance. Thanks in advance, Karen Bauer HT(ASCP) Histology Supervisor Luther Hospital Eau Claire, WI ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. From MElliott <@t> mrl.ubc.ca Tue Oct 16 12:54:06 2007 From: MElliott <@t> mrl.ubc.ca (Mark Elliott) Date: Tue Oct 16 12:54:49 2007 Subject: [Histonet] PAS for frozens Message-ID: <4714984E020000D600027301@mail.mrl.ubc.ca> Does anyone have a procedure for doing a PAS on frozen tissue?? I assume we need to shorten the times in the various regents, but by how much? Any tips/tricks would be greatly appreciated. We are staining human lung tissue. Thanks Mark in dreary Vancouver BC ***CONFIDENTIALITY NOTICE*** This electronic message is intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From Marirose.Satterfield <@t> MercyMemorial.org Tue Oct 16 12:57:21 2007 From: Marirose.Satterfield <@t> MercyMemorial.org (Satterfield, Marirose) Date: Tue Oct 16 13:04:27 2007 Subject: [Histonet] Formalin Message-ID: We are going to be switching to Formalin. Our local water department has informed us that we cannot dump the Formalin down the drain. Could I hear some feed back on how you handle your disposal- waste haulers vs. recycling. Thanks Mari Marirose Satterfield Histology Supervisor Mercy Memorial Hospital From Shirley_PHUA <@t> hsa.gov.sg Tue Oct 16 13:03:41 2007 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Tue Oct 16 13:06:54 2007 Subject: [Histonet] Shirley Phua is away on course 17-18 October 2007. Message-ID: I will be out of the office from 17-10-2007 to 18-10-2007. I'll be away on course 17-18 October 2007. I'll be back on 19 October 2007. Pathologists: I will process your requests when I return. If urgent, please forward your email to Henry_Kyaw@hsa.gov.sg From ploykasek <@t> phenopath.com Tue Oct 16 13:22:52 2007 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Tue Oct 16 13:23:04 2007 Subject: [Histonet] Slide labeling pen Message-ID: Hi all. I need some help determining the manufacturer of a slide labeling pen. I received a free sample, and now can't remember where it came from (must be the fumes attacking my brain). The pen is labeled as KP Marker Plus. On our slides it has held up well to deparaffinization reagents, heat pretreatment, etc... Thanks for the help. I'm much chagrined having to post this. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From sheila_adey <@t> hotmail.com Tue Oct 16 13:34:05 2007 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Tue Oct 16 13:34:12 2007 Subject: [Histonet] Slide labeling pen In-Reply-To: References: Message-ID: Hi Patti, We love them to. They are from Mercedes Medical. Sheila Adey HT MLTPort Huron HospitalMichigan> Date: Tue, 16 Oct 2007 11:22:52 -0700> From: ploykasek@phenopath.com> To: histonet@pathology.swmed.edu> CC: > Subject: [Histonet] Slide labeling pen> > Hi all. I need some help determining the manufacturer of a slide labeling> pen. I received a free sample, and now can't remember where it came from> (must be the fumes attacking my brain). The pen is labeled as KP Marker> Plus. On our slides it has held up well to deparaffinization reagents, heat> pretreatment, etc...> Thanks for the help. I'm much chagrined having to post this.> > > Patti Loykasek BS, HTL, QIHC> PhenoPath Laboratories> Seattle, WA> > > > -------------------------------------------------------------------------> This e-mail message, including any attachments, is for the sole use of> the intended recipients and may contain privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. If > you are not the intended recipient, please contact the sender by e-mail > and destroy all copies of the original message, or you may call PhenoPath > Laboratories, Seattle, WA U.S.A. at (206) 374-9000.> > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Are you ready for Windows Live Messenger Beta 8.5 ? Get the latest for free today! http://entertainment.sympatico.msn.ca/WindowsLiveMessenger From liz <@t> premierlab.com Tue Oct 16 13:53:04 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Tue Oct 16 13:53:08 2007 Subject: [Histonet] Slide labeling pen In-Reply-To: <149C874907274170BC97DD9FB41E8748@PremierLab.local> References: <149C874907274170BC97DD9FB41E8748@PremierLab.local> Message-ID: I thinks it mercedes medical Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti Loykasek Sent: Tuesday, October 16, 2007 12:45 PM To: histonet Subject: [Histonet] Slide labeling pen Hi all. I need some help determining the manufacturer of a slide labeling pen. I received a free sample, and now can't remember where it came from (must be the fumes attacking my brain). The pen is labeled as KP Marker Plus. On our slides it has held up well to deparaffinization reagents, heat pretreatment, etc... Thanks for the help. I'm much chagrined having to post this. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------ - This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From djohnson <@t> mercedesmedical.com Tue Oct 16 14:03:31 2007 From: djohnson <@t> mercedesmedical.com (Dave Johnson) Date: Tue Oct 16 14:03:35 2007 Subject: [Histonet] Slide labeling pen Message-ID: <46D38D90616923469B1C86663FC1327402466500@mailserver.mercedesmedical.com> Yes, it is indeed Mercedes Medical Give us a call. 800-331-2716 Dave Johnson -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Tuesday, October 16, 2007 2:53 PM To: Patti Loykasek; histonet Subject: RE: [Histonet] Slide labeling pen I thinks it mercedes medical Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti Loykasek Sent: Tuesday, October 16, 2007 12:45 PM To: histonet Subject: [Histonet] Slide labeling pen Hi all. I need some help determining the manufacturer of a slide labeling pen. I received a free sample, and now can't remember where it came from (must be the fumes attacking my brain). The pen is labeled as KP Marker Plus. On our slides it has held up well to deparaffinization reagents, heat pretreatment, etc... Thanks for the help. I'm much chagrined having to post this. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------ - This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dmccaig <@t> ckha.on.ca Tue Oct 16 14:03:47 2007 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Tue Oct 16 14:03:55 2007 Subject: [Histonet] FUNGI CONTROL BLOCK Message-ID: Does someone have a good fungus control block that demonstrates hyphae? Would be truly appreciated Diana McCaig Histology Lab Chatham Kent Health Alliance Chatham. Ontario N8A 5E1 519-352-6401 (6604) From gentras <@t> vetmed.auburn.edu Tue Oct 16 14:54:39 2007 From: gentras <@t> vetmed.auburn.edu (Atoska Gentry) Date: Tue Oct 16 14:54:47 2007 Subject: [Histonet] frozen sections Message-ID: <471516FF.8000108@vetmed.auburn.edu> hello, has anyone observed empty space surrounding tissue frozen in O.C.T. or similar tissue freezing medium after snap freezing? Upon sectioning samples shipped previously snap frozen to one of our researchers I noticed space surrounding various areas of the tissue. Does anyone know what probably caused this? Could this be a factor in the generation of horrible sections? I suspect the tissue may be moving when the blade touches it. Is there a know remedy that will not damage the tissue? Has anyone ever added O.C.T. to already frozen tissues? Any assistance will be much appreciated. Atoska -- Atoska S. Gentry, B.S., HT(ASCP) Research Assistant IV Scott-Ritchey RSCH Center College of Vet. Med Auburn, AL 36849 PH (334) 844-5579 FAX (334) 844-5850 email: gentras@vetmed.auburn.edu From JWEEMS <@t> sjha.org Tue Oct 16 14:58:01 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Tue Oct 16 14:58:17 2007 Subject: [Histonet] frozen sections In-Reply-To: <471516FF.8000108@vetmed.auburn.edu> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F3F38@sjhaexc02.sjha.org> Please the tissue in baby powder and it will eliminate the air bubble. You can also add baby powder to the OCT and it will give it more "body" for holding the tissue correctly for freezing. I don't remember where I heard this or learned it... I just know it works! Hope it works for you, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Atoska Gentry Sent: Tuesday, October 16, 2007 3:55 PM To: Histonet Subject: [Histonet] frozen sections hello, has anyone observed empty space surrounding tissue frozen in O.C.T. or similar tissue freezing medium after snap freezing? Upon sectioning samples shipped previously snap frozen to one of our researchers I noticed space surrounding various areas of the tissue. Does anyone know what probably caused this? Could this be a factor in the generation of horrible sections? I suspect the tissue may be moving when the blade touches it. Is there a know remedy that will not damage the tissue? Has anyone ever added O.C.T. to already frozen tissues? Any assistance will be much appreciated. Atoska -- Atoska S. Gentry, B.S., HT(ASCP) Research Assistant IV Scott-Ritchey RSCH Center College of Vet. Med Auburn, AL 36849 PH (334) 844-5579 FAX (334) 844-5850 email: gentras@vetmed.auburn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From rjbuesa <@t> yahoo.com Tue Oct 16 15:34:22 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Oct 16 15:34:30 2007 Subject: [Histonet] frozen sections In-Reply-To: <471516FF.8000108@vetmed.auburn.edu> Message-ID: <846676.2416.qm@web61211.mail.yahoo.com> Yes, if there is a space beyween the OCT and the tissue, you can "refill" it and freeze it again. Ren? J. Atoska Gentry wrote: hello, has anyone observed empty space surrounding tissue frozen in O.C.T. or similar tissue freezing medium after snap freezing? Upon sectioning samples shipped previously snap frozen to one of our researchers I noticed space surrounding various areas of the tissue. Does anyone know what probably caused this? Could this be a factor in the generation of horrible sections? I suspect the tissue may be moving when the blade touches it. Is there a know remedy that will not damage the tissue? Has anyone ever added O.C.T. to already frozen tissues? Any assistance will be much appreciated. Atoska -- Atoska S. Gentry, B.S., HT(ASCP) Research Assistant IV Scott-Ritchey RSCH Center College of Vet. Med Auburn, AL 36849 PH (334) 844-5579 FAX (334) 844-5850 email: gentras@vetmed.auburn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Catch up on fall's hot new shows on Yahoo! TV. Watch previews, get listings, and more! From ccross6032 <@t> aol.com Tue Oct 16 15:39:04 2007 From: ccross6032 <@t> aol.com (Cheryl Cross) Date: Tue Oct 16 15:39:29 2007 Subject: [Histonet] crazy immuno question - can you restain slides? Message-ID: <009D9AB1-E76A-45DD-83F1-3ABE7BEE760D@aol.com> hi everybody - I'm going back through some of my earlier attempts at immuno for cleaved caspase 3; i have a beautiful lesion i ruined :) on dilutions. is there anyway i can use my newer, better protocol on this previously stained slide? seems such a waste :) thanks for any input! CC Cheryl Cross, DVM, Dipl. ACVP Researcher University Corporation for Atmospheric Research College of Veterinary Medicine University of Tennessee Department of Pathology 2407 River Drive, Room A201 Knoxville, TN 37996-4542 (423) 967-2724 fax: 865-974-5616 ccross@ucar.edu From rjbuesa <@t> yahoo.com Tue Oct 16 15:58:04 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Oct 16 15:58:08 2007 Subject: [Histonet] crazy immuno question - can you restain slides? In-Reply-To: <009D9AB1-E76A-45DD-83F1-3ABE7BEE760D@aol.com> Message-ID: <474958.73016.qm@web61213.mail.yahoo.com> If the section already has DAB (as it probably does), you cannot eliminate it. Ren? J. Cheryl Cross wrote: hi everybody - I'm going back through some of my earlier attempts at immuno for cleaved caspase 3; i have a beautiful lesion i ruined :) on dilutions. is there anyway i can use my newer, better protocol on this previously stained slide? seems such a waste :) thanks for any input! CC Cheryl Cross, DVM, Dipl. ACVP Researcher University Corporation for Atmospheric Research College of Veterinary Medicine University of Tennessee Department of Pathology 2407 River Drive, Room A201 Knoxville, TN 37996-4542 (423) 967-2724 fax: 865-974-5616 ccross@ucar.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Need a vacation? Get great deals to amazing places on Yahoo! Travel. From ccross6032 <@t> aol.com Tue Oct 16 16:12:20 2007 From: ccross6032 <@t> aol.com (Cheryl Cross) Date: Tue Oct 16 16:12:31 2007 Subject: [Histonet] crazy immuno question - can you restain slides? In-Reply-To: <474958.73016.qm@web61213.mail.yahoo.com> References: <474958.73016.qm@web61213.mail.yahoo.com> Message-ID: <980C596F-9F85-4078-B277-D9A8844CB217@aol.com> i see - thanks Bonnie and Rene for your input... The old trial sections have not only a lower dilution that didn't work, but a completely different antibody. the rest of the protocol would be the same. it sounds as though if my old sections were understained it may be worth a shot.....but no guarantees :) if i'm understanding you correctly. thanks! CC Cheryl Cross, DVM, Dipl. ACVP Researcher University Corporation for Atmospheric Research College of Veterinary Medicine University of Tennessee Department of Pathology 2407 River Drive, Room A201 Knoxville, TN 37996-4542 (423) 967-2724 fax: 865-974-5616 ccross@ucar.edu On Oct 16, 2007, at 4:58 PM, Rene J Buesa wrote: > If the section already has DAB (as it probably does), you cannot > eliminate it. > Ren? J. > > Cheryl Cross wrote: > hi everybody - > > I'm going back through some of my earlier attempts at immuno for > cleaved caspase 3; i have a beautiful lesion i ruined :) on > dilutions. is there anyway i can use my newer, better protocol on > this previously stained slide? > > seems such a waste :) > > thanks for any input! > > CC > > Cheryl Cross, DVM, Dipl. ACVP > Researcher > University Corporation for Atmospheric Research > College of Veterinary Medicine > University of Tennessee Department of Pathology > 2407 River Drive, Room A201 > Knoxville, TN 37996-4542 > (423) 967-2724 > fax: 865-974-5616 > ccross@ucar.edu > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > Need a vacation? Get great deals to amazing places on Yahoo! Travel. From ploykasek <@t> phenopath.com Tue Oct 16 16:46:34 2007 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Tue Oct 16 16:46:40 2007 Subject: [Histonet] Slide labeling pens Message-ID: Thanks to all for the answers to my slide labeling pen question. The KP Marker pens are distributed by Mercedes Medical. Unfortunately, the pens are currently on back order. Call & get your name on the list. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From mickie25 <@t> netzero.net Tue Oct 16 18:13:59 2007 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Tue Oct 16 18:14:34 2007 Subject: [Histonet] frozen sections In-Reply-To: <471516FF.8000108@vetmed.auburn.edu> References: <471516FF.8000108@vetmed.auburn.edu> Message-ID: This may be remedied by smearing some OCT to the face of the frozen block. One caution is that this may cause the formation of freezing artifact that looks like small holes next to nuclei or in the center of muscle fibers from the thawing that occurs when adding warm OCT to the frozen block face which then re-freezes causing ice crystals to form. The effect may not go deep in the block though. Good luck. Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Atoska Gentry Sent: Tuesday, October 16, 2007 12:55 PM To: Histonet Subject: [Histonet] frozen sections hello, has anyone observed empty space surrounding tissue frozen in O.C.T. or similar tissue freezing medium after snap freezing? Upon sectioning samples shipped previously snap frozen to one of our researchers I noticed space surrounding various areas of the tissue. Does anyone know what probably caused this? Could this be a factor in the generation of horrible sections? I suspect the tissue may be moving when the blade touches it. Is there a know remedy that will not damage the tissue? Has anyone ever added O.C.T. to already frozen tissues? Any assistance will be much appreciated. Atoska -- Atoska S. Gentry, B.S., HT(ASCP) Research Assistant IV Scott-Ritchey RSCH Center College of Vet. Med Auburn, AL 36849 PH (334) 844-5579 FAX (334) 844-5850 email: gentras@vetmed.auburn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From magicchessa <@t> yahoo.com Tue Oct 16 19:43:26 2007 From: magicchessa <@t> yahoo.com (Malissa Snyder) Date: Tue Oct 16 19:43:30 2007 Subject: [Histonet] formalin disposal Message-ID: <601608.92888.qm@web50208.mail.re2.yahoo.com> We actually have a powder neutralizer that we put in the jugs of used formalin and then we are able by state and county to pour it down the drain. Maybe, they will let you do the same. --------------------------------- Yahoo! oneSearch: Finally, mobile search that gives answers, not web links. From Megan.Clarke <@t> hnehealth.nsw.gov.au Tue Oct 16 22:08:18 2007 From: Megan.Clarke <@t> hnehealth.nsw.gov.au (Megan Clarke) Date: Tue Oct 16 22:08:53 2007 Subject: [Histonet] C9 antibody Message-ID: <47160942020000250001A37A@domainatrix.HAHS.HEALTH.NSW.GOV.AU> Hi Histonetters We are looking for an antibody to Complement 9 that can be used for Immunohistochemistry stains on formalin fixed paraffin embedded sections. If there is a company out there that supplies this antibody or someone that already uses this antibody, could you please forward this information to us. A distributor in australia would be helpful. Thanking you in anticipation. Zenobia Haffajee HAPS Newcastle Australia From valeria.berno <@t> embl.it Wed Oct 17 03:00:04 2007 From: valeria.berno <@t> embl.it (Valeria Berno) Date: Wed Oct 17 03:00:23 2007 Subject: [Histonet] cross reaction Message-ID: <1127.10.251.1.146.1192608004.squirrel@10.251.1.146> Hi, I am working with a user on neurons culture (embryonic) in order to characterize a list of antibodies for differentiated and undifferentiated markers. Here the problem: either transcription factor (nuclear) and membrane antigens show staining in the nuclei and in the membrane. it seems the antibody sticks on the membrane or enter partially in the nuclei. The protocol is 4%PFA 10min RT, triton 0.3% 10min, bl0cking in Normal goat serum,and washes in TBS-tween0.1%. Any suggestions? Thanks in advance Valeria Berno Valeria, PhD EMBL- Mouse Biology Unit Campus A. Buzzati-Traverso Via Ramarini, 32 00015, Monterotondo Scalo (RM) Italy Tel: +39 06 90091287 Fax: +39 06 90091406 email: valeria.berno@embl.it www.embl.it From mickie25 <@t> netzero.net Wed Oct 17 05:52:40 2007 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Wed Oct 17 05:53:18 2007 Subject: [Histonet] Formalin In-Reply-To: References: Message-ID: Creative Waste Solutions in Portland (888)795-8300 has a filtration system which conserves the buffer salts and so greatly extends the useful life of buffered formalin. Give them a call for more details. Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Satterfield, Marirose Sent: Tuesday, October 16, 2007 10:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin We are going to be switching to Formalin. Our local water department has informed us that we cannot dump the Formalin down the drain. Could I hear some feed back on how you handle your disposal- waste haulers vs. recycling. Thanks Mari Marirose Satterfield Histology Supervisor Mercy Memorial Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From christina_bisgaard <@t> yahoo.dk Wed Oct 17 06:28:13 2007 From: christina_bisgaard <@t> yahoo.dk (christina bisgaard) Date: Wed Oct 17 06:28:18 2007 Subject: [Histonet] Laser Capture Microdissection and thick rat brain sections Message-ID: <94261.79139.qm@web54410.mail.yahoo.com> Dear Histonetters I?m in urgent need of help Does anyone out there have experience with laser capture micro-dissection (Arcturus, Veritas) and thick (60-80?) rat brain section? I have problems collecting more than one Dentate Gyrus (DG) subregion from the hippocampal area from rat brain sections onto the macro cap. I have tried both PEN membrane glass slides and FRAME slides. Could this be due to lack of dehydration? When staining/dehydrating 60? horizontal sections, I observe severe horizontal tearing of the sections if left for more than 30 sec in each dehydration step. If put in Xylene, they are totally broken. Even at 30 sec we sometimes see ?blackness? of parts of the sections. My staining protocol is: (using this protocol we don?t see much horizontal tearing) 1) Directly from -80oC to 75% EtOH, 30sec 2) dH2O, 30sec 3) Stain, Toluidin Blue, 3 min. 4) dH2O, 30sec 5) 50% EtOH, 30sec 6) 75% EtOH, 30sec 7) 96% EtOH, 30 sec 8) 100% EtOH, 30sec I wish to utilize the cap as much as possible (up to 8 DG per cap) in part for economic reasons. Since the downstream process is 2D gels and protein analysis I need as much tissue from each brain as possible which is why I wish to use thick sections to decrease the number of sections to be microdissected. I?m kind of stuck right now and running out of ideas. I will appreciate any help and will provide more information concerning our protocol if needed. Christina Bisgaard, PhD Stud., Center for Psychiatric Research, Denmark --------------------------------- F? en billig laptop. Se Kelkoos gode tilbud her! From kgreen <@t> hsh.org Wed Oct 17 07:05:45 2007 From: kgreen <@t> hsh.org (Green, Kathy) Date: Wed Oct 17 07:05:51 2007 Subject: [Histonet] question about charges Message-ID: Does anyone have "proof" in regard to the ruling of charging multiple specimens? For example, currently if we get a specimen slip with a uterus, non neoplastic (88307) & an appendix, we can only charge for the "higher" item. Our new director is sure that where he was before they were able to charge for multiple specimens. Our pathologists bill for multiple specimens, but out billing dept. says that we aren't allowed. So if anyone out there does "charge" for multiple specimens like that & have "proof" or something that I can show to billing, I would greatly appreciate it. Kathy Green, HT Manager Histology Laboratory Holy Spirit Hospital 503 N. 21st Street Camp Hill, PA 17011 kgreen@hsh.org (717) 763-2930 Blackberry: (717) 370-1726 Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You From sbledsoe <@t> iupui.edu Wed Oct 17 07:32:49 2007 From: sbledsoe <@t> iupui.edu (Sharon Bledsoe) Date: Wed Oct 17 07:32:56 2007 Subject: [Histonet] frozen sections In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F3F38@sjhaexc02.sjha.org> References: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F3F38@sjhaexc02.sjha.org> Message-ID: Joyce, How does the baby powder effect your sample if you have to use birefringence? Talc polarizes, I have to be careful and use non-powdered gloves when I section many of our animal tissue as we are looking for crystals that are identified by birefringence. Do you note on the slide that you have an additive on a frozen section? Sharon Bledsoe At 3:58 PM -0400 10/16/07, Weems, Joyce wrote: >Content-class: urn:content-classes:message >Content-Type: text/plain; > charset="utf-8" > >Please the tissue in baby powder and it will eliminate the air >bubble. You can also add baby powder to the OCT and it will give it >more "body" for holding the tissue correctly for freezing. I don't >remember where I heard this or learned it... I just know it works! >Hope it works for you, j > >Joyce Weems >Pathology Manager >Saint Joseph's Hospital >5665 Peachtree Dunwoody Rd NE >Atlanta, GA 30342 >404-851-7376 - Phone >404-851-7831 - Fax > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Atoska >Gentry >Sent: Tuesday, October 16, 2007 3:55 PM >To: Histonet >Subject: [Histonet] frozen sections > > >hello, has anyone observed empty space surrounding tissue frozen in >O.C.T. or similar tissue freezing medium after snap freezing? Upon >sectioning samples shipped previously snap frozen to one of our >researchers I noticed space surrounding various areas of the tissue. >Does anyone know what probably caused this? Could this be a factor in >the generation of horrible sections? I suspect the tissue may be moving >when the blade touches it. Is there a know remedy that will not damage >the tissue? Has anyone ever added O.C.T. to already frozen tissues? Any >assistance will be much appreciated. Atoska > >-- >Atoska S. Gentry, B.S., HT(ASCP) >Research Assistant IV >Scott-Ritchey RSCH Center >College of Vet. Med >Auburn, AL 36849 >PH (334) 844-5579 >FAX (334) 844-5850 >email: gentras@vetmed.auburn.edu > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >Confidentiality Notice ** The information contained in this message >may be privileged and is confidential information intended for the >use of the addressee listed above. If you are neither the intended >recipient nor the employee or agent responsible for delivering this >message to the intended recipient, you are hereby notified that any >disclosure, copying, distribution or the taking of any action in >reliance on the contents of this information is strictly prohibited. >If you have received this communication in error, please notify us >immediately by replying to the message and deleting it from your >computer. Thank you. Saint Joseph's Health System, Inc. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Wed Oct 17 08:02:48 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Oct 17 08:03:07 2007 Subject: [Histonet] question about charges In-Reply-To: Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F3F41@sjhaexc02.sjha.org> It's in the CPT code guidelines. There are some specimens that need to be "bundled" for charging, but if you have separate specimens that require separate diagnoses, each specimen gets a separate charge. Yes, you would charge for the uterus - depending on whether it is malignant or not and an 88302 for an incidental appendix. If you have uterus, tubes, and ovaries, there are guidelines about bundling those specimens - sometimes you don't. There are many good resources for billing. Dennis Padget is a good one. He has good instructions and answers for all questions. Our pathologists have subscribed to his services. Good luck. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Green, Kathy Sent: Wednesday, October 17, 2007 8:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] question about charges Does anyone have "proof" in regard to the ruling of charging multiple specimens? For example, currently if we get a specimen slip with a uterus, non neoplastic (88307) & an appendix, we can only charge for the "higher" item. Our new director is sure that where he was before they were able to charge for multiple specimens. Our pathologists bill for multiple specimens, but out billing dept. says that we aren't allowed. So if anyone out there does "charge" for multiple specimens like that & have "proof" or something that I can show to billing, I would greatly appreciate it. Kathy Green, HT Manager Histology Laboratory Holy Spirit Hospital 503 N. 21st Street Camp Hill, PA 17011 kgreen@hsh.org (717) 763-2930 Blackberry: (717) 370-1726 Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From JWEEMS <@t> sjha.org Wed Oct 17 08:05:05 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Oct 17 08:05:27 2007 Subject: [Histonet] Formalin In-Reply-To: Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F3F42@sjhaexc02.sjha.org> We use the Neutralex system from Sakura. They are certified approved with the state of CA. I rest assured that if CA approves, we are safe to us anywhere! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Mickie Johnson Sent: Wednesday, October 17, 2007 6:53 AM To: 'Satterfield, Marirose'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Formalin Creative Waste Solutions in Portland (888)795-8300 has a filtration system which conserves the buffer salts and so greatly extends the useful life of buffered formalin. Give them a call for more details. Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Satterfield, Marirose Sent: Tuesday, October 16, 2007 10:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin We are going to be switching to Formalin. Our local water department has informed us that we cannot dump the Formalin down the drain. Could I hear some feed back on how you handle your disposal- waste haulers vs. recycling. Thanks Mari Marirose Satterfield Histology Supervisor Mercy Memorial Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From JWEEMS <@t> sjha.org Wed Oct 17 08:08:37 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Oct 17 08:08:57 2007 Subject: [Histonet] frozen sections In-Reply-To: Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F3F44@sjhaexc02.sjha.org> I never used it for fluorescence, so I don't know! Sounds like that might be a problem for you. I used it for muscles and would freeze them on the chuck. I rolled the tissue in the powder and thickened the OCT with it. They stood on end for freezing and I was never aware of any problem caused by the talc. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: Sharon Bledsoe [mailto:sbledsoe@iupui.edu] Sent: Wednesday, October 17, 2007 8:33 AM To: Weems, Joyce; Atoska Gentry; Histonet Subject: RE: [Histonet] frozen sections Joyce, How does the baby powder effect your sample if you have to use birefringence? Talc polarizes, I have to be careful and use non-powdered gloves when I section many of our animal tissue as we are looking for crystals that are identified by birefringence. Do you note on the slide that you have an additive on a frozen section? Sharon Bledsoe At 3:58 PM -0400 10/16/07, Weems, Joyce wrote: >Content-class: urn:content-classes:message >Content-Type: text/plain; > charset="utf-8" > >Please the tissue in baby powder and it will eliminate the air >bubble. You can also add baby powder to the OCT and it will give it >more "body" for holding the tissue correctly for freezing. I don't >remember where I heard this or learned it... I just know it works! >Hope it works for you, j > >Joyce Weems >Pathology Manager >Saint Joseph's Hospital >5665 Peachtree Dunwoody Rd NE >Atlanta, GA 30342 >404-851-7376 - Phone >404-851-7831 - Fax > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Atoska >Gentry >Sent: Tuesday, October 16, 2007 3:55 PM >To: Histonet >Subject: [Histonet] frozen sections > > >hello, has anyone observed empty space surrounding tissue frozen in >O.C.T. or similar tissue freezing medium after snap freezing? Upon >sectioning samples shipped previously snap frozen to one of our >researchers I noticed space surrounding various areas of the tissue. >Does anyone know what probably caused this? Could this be a factor in >the generation of horrible sections? I suspect the tissue may be moving >when the blade touches it. Is there a know remedy that will not damage >the tissue? Has anyone ever added O.C.T. to already frozen tissues? Any >assistance will be much appreciated. Atoska > >-- >Atoska S. Gentry, B.S., HT(ASCP) >Research Assistant IV >Scott-Ritchey RSCH Center >College of Vet. Med >Auburn, AL 36849 >PH (334) 844-5579 >FAX (334) 844-5850 >email: gentras@vetmed.auburn.edu > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >Confidentiality Notice ** The information contained in this message >may be privileged and is confidential information intended for the >use of the addressee listed above. If you are neither the intended >recipient nor the employee or agent responsible for delivering this >message to the intended recipient, you are hereby notified that any >disclosure, copying, distribution or the taking of any action in >reliance on the contents of this information is strictly prohibited. >If you have received this communication in error, please notify us >immediately by replying to the message and deleting it from your >computer. Thank you. Saint Joseph's Health System, Inc. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From GDeville <@t> hlm-la.com Wed Oct 17 08:51:15 2007 From: GDeville <@t> hlm-la.com (Gwen R. Deville) Date: Wed Oct 17 08:51:18 2007 Subject: [Histonet] Surgipath semi-automatic microtomes Message-ID: <9D0C87254B13304FAB8887A15EA9522DE114@hlmlasbs.hlm-la.local> We have 3 Surgipath Model#4800E semi-automatic microtomes which are approximately 5 to 6 years old. When we first purchased them, they cut great, however, for about the last year to present day, we have experienced great difficultly with microtomy. You can be cutting perfectly for several hours then all of a sudden you can not get a ribbon at all or better yet, it may cut great the day before, then not cut at all the next day when you start. We have not made any changes in our cutting process (we use Accu Edge blades and cut routinely on 4 microns, with the angle normally set somewhere between 1-3). We have regular preventive maintenance performed on each instrument. In the past we've had issue with the blade holder screws backing out of the knife holder and are in the process of having the holders rebuilt by Surgipath. We feel like this is probably the source of our problems. Just wondering if any one else has experienced issues with these microtomes or has any suggestions. Gwen Deville, Histology Supv. Delta Pathology, Mid-Louisiana Box 30113, 211 Fourth Street Alexandria, LA 71301 Work: (318)473-3943 / (318) 473-3180 Mobile: (318) 729-4203 Pager: (318) 427-5444 Email: gdeville@hlm-la.com NOTICE: This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissenmination, distribution, forwarding, printing, or copying of the email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. From SDrew <@t> uwhealth.org Wed Oct 17 09:09:18 2007 From: SDrew <@t> uwhealth.org (Drew Sally A.) Date: Wed Oct 17 09:09:26 2007 Subject: [Histonet] C9 antibody In-Reply-To: <47160942020000250001A37A@domainatrix.HAHS.HEALTH.NSW.GOV.AU> Message-ID: Our C9 comes from Vision Biosystems/Novocastra (NCL-CCC9)and works well on our Ventana instruments for paraffin protocols. I'd be happy to give you the details if you'd like. Sally Ann Drew, MT(ASCP) IHC/ISH Laboratory University of Wisconsin Hosp. & Clinics Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Megan Clarke Sent: Tuesday, October 16, 2007 10:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] C9 antibody Hi Histonetters We are looking for an antibody to Complement 9 that can be used for Immunohistochemistry stains on formalin fixed paraffin embedded sections. If there is a company out there that supplies this antibody or someone that already uses this antibody, could you please forward this information to us. A distributor in australia would be helpful. Thanking you in anticipation. Zenobia Haffajee HAPS Newcastle Australia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Wed Oct 17 09:11:06 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Oct 17 09:11:15 2007 Subject: [Histonet] crazy immuno question - can you restain slides? In-Reply-To: <009D9AB1-E76A-45DD-83F1-3ABE7BEE760D@aol.com> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273CEE@LSRIEXCH1.lsmaster.lifespan.org> If the section was a paraffin section, and the original antibody didn't work - in other words the section is essentially unstained - then you should be able to stain it with another antibody using standard peroxidase-DAB. If there is any peroxidase-DAB pigment in the tissue from the original staining attempt, you won't be able to remove it, and will have to work around it, either by ignoring it if it is sufficiently light, or by using a different substrate this time, that will produce a different color. If the section was a frozen section, then dehydrating it after the original stain prior to coverslipping may have denatured the antigen to the point where it will no longer be recognized by the antibody. In that case you could try antigen retrievel, but I don't know how well that would work. From gu.lang <@t> gmx.at Wed Oct 17 09:36:47 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Oct 17 09:36:54 2007 Subject: AW: [Histonet] AutoStainer XL Tweaking In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B8F46DA@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <003601c810cb$262d7d20$6412a8c0@dielangs.at> Hi, this is my schedule: 25 min 60?C oven - 2x5 min Xylolsubstitute - each 2 min 96%-70%-50%-A.dest. - 10 min Mayers hemalaun - 3 min bluing in running tapwater - 45 sek. aqu. 2%eosin - - 30 sek wash in running tapwater - 30 sek 96% - each 1 min 3x100% - 1 min butylacetat - caverslipping Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Breeden, Sara Gesendet: Dienstag, 16. Oktober 2007 14:04 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] AutoStainer XL Tweaking I'm setting up my Leica AutoStainer XL and would appreciate any help with tweaking the times. I based my timing for the Autostainer on my manual stain times but staining is not crisp and looks washed out. Docs say eosin is fine but cellular detail is foggy. Here are my times: XYL - 3 at 5 min each 100% reagent - 2 at 30 seconds each 95% reagent - 1 at 30 seconds 80% reagent - 1 at 30 seconds DI water wash - 1 minute Hematox (currently using Sigma) - 5 minutes DI water wash - 1 minute Acid Alcohol (1%) - one dip DI water wash - 1 minute Ammonia water - 15 seconds DI water wash - 1 minute 80% reagent - 1 at 30 seconds 95% reagent - 1 at 30 seconds Eosin (alcoholic) - 10 seconds 95% reagent - 1 at 30 seconds 100% reagent - 2 at 1 minute each XYL - 3 at 1 minute each I cut my acid alcohol time down from 15 seconds to one dip but it's still taking a lot of hematoxylin off. Should I try 0.5% acid alcohol? I'm at a loss so far; different hematox? More time in ammonia water? If you use this Leica AutoStainer XL, could you offer some Handy Hints? I thank you SO much! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NMargaryan <@t> childrensmemorial.org Wed Oct 17 09:40:59 2007 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Wed Oct 17 09:39:00 2007 Subject: [Histonet] in situ Message-ID: Dear Histonetters, I would like to express my appreciation to everyone who has contributed with advices and suggestions in help with in situ hybridization. I am still working on it and looking forward to get results. If you have more to suggest I will be happy to get it:-) Have a nice day, Naira Naira V. Margaryan, D.V.M., Ph.D. Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3394 Tel: 773-755-6340 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 From Carmen.Wynn <@t> us.astellas.com Wed Oct 17 09:51:01 2007 From: Carmen.Wynn <@t> us.astellas.com (Wynn, Carmen) Date: Wed Oct 17 09:51:55 2007 Subject: [Histonet] RE: Polymer based IHC reagent for mouse antibodies on rat tissue In-Reply-To: <20071013170643.9C3D7658075@mail16-fra.bigfish.com> Message-ID: Hello Histonetters, I have had great success with the Biocare HRP polymer product line. I have used the various kits: Mouse Primary antibody on Rat tissue, Rat Primary Antibody on Mouse tissue, Rabbit Primary on Rodent tissue (for both mouse and rat), and Goat Primary antibody on Human, Mouse, and Rat tissue. All work great with no background and the protocols are very quick. Just don't forget to rinse in water prior to the substrate chromagen application...VERY IMPORTANT!! I use Romulin AEC at double strength for chromagen. They also offer all these reagents in the AP form as well. Good Luck! Carmen Wynn, M.S., Senior Scientist Astellas Research Institute of America, LLC. (ARIA) Illinois Science and Technology Park 8045 Lamon Ave Skokie, IL 60077 Direct: 847-933-7419 Main: 847-933-7400 Fax: 847-933-7401 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Saturday, October 13, 2007 12:07 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 47, Issue 15 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Teratogens (Rene J Buesa) 2. Re: Defrosting Cryostat (Rene J Buesa) 3. proventricular dilatation images? (I-sanna Gibbons) 4. Re: proventricular dilatation images? (Cheryl Cross) 5. Re: proventricular dilatation images? (I-sanna Gibbons) 6. Keeping frozen mouse aorta on slides (Randolph-Habecker, Julie) 7. polymer based IHC reagent for mouse antibodies on rat tissue (Liz Chlipala) 8. Re: Keeping frozen mouse aorta on slides (Rene J Buesa) 9. Keeping frozen mouse aorta on slides (Paula Pierce) 10. polymer based IHC reagent for mouse antibodies on rat tissue (Paula Pierce) ---------------------------------------------------------------------- Message: 1 Date: Fri, 12 Oct 2007 10:23:30 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Teratogens To: Diana McCaig , histonet@lists.utsouthwestern.edu Message-ID: <823783.2629.qm@web61214.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Diana: 1- teratogen, mutagen or any such an indication in the MSDS should be considered as warnings for the label 2- at any concentration 3- all pregnant employees should (and deserve) being assigned low risk activities, even when 31% of USA histology labs do not practice this prohibition 4- you could Google "Teratogen and Mutagen" Ren J. Diana McCaig wrote: Hi I was wondering if anyone specifically labels these chemicals in your lab to alert any techs of child producing age. Also, how do you establish this criteria, some could be in the concentrated form or powder state, but do they still qualify in a dilute state? Is this determined strictly by the MSDS and based on the word teratogen or do you also consider terminology as mutagen, reproductive effector as well? If protective equipment and proper ventilation is available, are pregnant staff exempt for doing any activity or stain that uses these solutions for the full term of their pregnancy and "I want to become pregnant" stages? And finally, can another provide a list of the chemicals they class as teratogens. thanks Diana McCaig _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better Heartthrob. Get better relationship answers from someone who knows. Yahoo! Answers - Check it out. ------------------------------ Message: 2 Date: Fri, 12 Oct 2007 10:24:59 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Defrosting Cryostat To: "Piche-Grocki, Jessica" , histonet@lists.utsouthwestern.edu Message-ID: <709197.83606.qm@web61222.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Cryostats should be defrosted daily, near midnight. Ren J. "Piche-Grocki, Jessica" wrote: Hi, How often should we defrost our Cryostat? I have set it up for once a week. Should it be more often? Thanks, Jessica Piche-Grocki, HT(ASCP) CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Building a website is a piece of cake. Yahoo! Small Business gives you all the tools to get online. ------------------------------ Message: 3 Date: Fri, 12 Oct 2007 11:04:36 -0700 (PDT) From: I-sanna Gibbons Subject: [Histonet] proventricular dilatation images? To: histonet@lists.utsouthwestern.edu Message-ID: <361313.20681.qm@web50310.mail.re2.yahoo.com> Content-Type: text/plain; charset=us-ascii Hi all, Does anyone have histological images of proventricular dilatation and also images of the normal for comparison? Thanks I-sanna ________________________________________________________________________ ____________ Moody friends. Drama queens. Your life? Nope! - their life, your story. Play Sims Stories at Yahoo! Games. http://sims.yahoo.com/ ------------------------------ Message: 4 Date: Fri, 12 Oct 2007 14:27:07 -0400 From: Cheryl Cross Subject: Re: [Histonet] proventricular dilatation images? To: I-sanna Gibbons Cc: histonet@lists.utsouthwestern.edu Message-ID: <05462208-2622-43F5-95C0-52961EF6A3A0@aol.com> Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed Hi I-sanna - I don't have any images readily available - there is a nice little review with some histopathology here, however: http://www.vet.uga.edu/vpp/ivcvm/1998/gregory/index.php What I can tell you is that in many cases can be a very very subtle disease - often there are just a few lymphocytes around the nerve ganglia - very very subtle. if you look at just a normal proventriculus histology book - an animal with really remarkable with have a thinned, stretched out proventriculus, but the histology can look very similar. It's easier to diagnose grossly (in my experience anyway), because the amount of inflammation histopathologically doesn't always match up with the degree of dilatation (ie animals with a marked dilation can have minimal inflammation). make sense? CC Cheryl Cross, DVM, Dipl. ACVP Researcher University Corporation for Atmospheric Research College of Veterinary Medicine University of Tennessee Department of Pathology 2407 River Drive, Room A201 Knoxville, TN 37996-4542 (423) 967-2724 fax: 865-974-5616 ccross@ucar.edu On Oct 12, 2007, at 2:04 PM, I-sanna Gibbons wrote: > Hi all, > > Does anyone have histological images of proventricular dilatation > and also images of the normal for comparison? > > Thanks > I-sanna > > > > ______________________________________________________________________ > ______________ > Moody friends. Drama queens. Your life? Nope! - their life, your > story. Play Sims Stories at Yahoo! Games. > http://sims.yahoo.com/ > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Fri, 12 Oct 2007 12:21:39 -0700 (PDT) From: I-sanna Gibbons Subject: Re: [Histonet] proventricular dilatation images? To: Cheryl Cross Cc: histonet@lists.utsouthwestern.edu Message-ID: <24335.52735.qm@web50301.mail.re2.yahoo.com> Content-Type: text/plain; charset=us-ascii Cheryl Yes it does! Thanks. Will check the website I-sanna ----- Original Message ---- From: Cheryl Cross To: I-sanna Gibbons Cc: histonet@lists.utsouthwestern.edu Sent: Friday, October 12, 2007 3:27:07 PM Subject: Re: [Histonet] proventricular dilatation images? Hi I-sanna - I don't have any images readily available - there is a nice little review with some histopathology here, however: http://www.vet.uga.edu/vpp/ivcvm/1998/gregory/index.php What I can tell you is that in many cases can be a very very subtle disease - often there are just a few lymphocytes around the nerve ganglia - very very subtle. if you look at just a normal proventriculus histology book - an animal with really remarkable with have a thinned, stretched out proventriculus, but the histology can look very similar. It's easier to diagnose grossly (in my experience anyway), because the amount of inflammation histopathologically doesn't always match up with the degree of dilatation (ie animals with a marked dilation can have minimal inflammation). make sense? CC Cheryl Cross, DVM, Dipl. ACVP Researcher University Corporation for Atmospheric Research College of Veterinary Medicine University of Tennessee Department of Pathology 2407 River Drive, Room A201 Knoxville, TN 37996-4542 (423) 967-2724 fax: 865-974-5616 ccross@ucar.edu On Oct 12, 2007, at 2:04 PM, I-sanna Gibbons wrote: Hi all, Does anyone have histological images of proventricular dilatation and also images of the normal for comparison? Thanks I-sanna ________________________________________________________________________ ____________ Moody friends. Drama queens. Your life? Nope! - their life, your story. Play Sims Stories at Yahoo! Games. http://sims.yahoo.com/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet = ________________________________________________________________________ ____________ Check out the hottest 2008 models today at Yahoo! Autos. http://autos.yahoo.com/new_cars.html ------------------------------ Message: 6 Date: Fri, 12 Oct 2007 15:18:40 -0700 From: "Randolph-Habecker, Julie" Subject: [Histonet] Keeping frozen mouse aorta on slides To: Message-ID: <040346FA7309BD439C327F97D4C4D69B13F127@ISIS.fhcrc.org> Content-Type: text/plain; charset="us-ascii" Folks, I need your suggestions! I am working with an investigator who wants my lab to stain unfixed, OCT embedded mouse aortas. Unfortunately, they have already cut all of the tissue so we do not have a choice of slides. They were cut at 6 microns on Star frost slides and stored at -80C for a few months. When we received them, we took a few slides out, let them air dry at room temperature for 24 hours, put them in a slides rack, soaked them in room temperature PBS for 5 minutes and fixed them with 10% NBF for 10 minutes. These steps are done without agitation. This procedure is compatible with our staining protocol and normally works fine when we use Super frost or Gold Plus slides from Erie. However, in this case, the tissue is floating off the slides in both the PBS and the NBF stage. I have also tried to do these steps with the slides flat and get the same results. Any suggestion on ways to "glue" already cut frozen sections to a charged slide in ways that are compatible with x-gal and IHC staining? Thanks in advance for your help!!!! Julie Julie Randolph-Habecker, Ph.D. Staff Scientist - Director Experimental Histopathology Shared Resource Fred Hutchinson Cancer Research Center 1100 Fairview Ave, N. M5-A803 Seattle WA 98109-1024 206-667-6119 jhabecke@fhcrc.org ------------------------------ Message: 7 Date: Fri, 12 Oct 2007 16:28:14 -0600 From: "Liz Chlipala" Subject: [Histonet] polymer based IHC reagent for mouse antibodies on rat tissue To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hello all Is anyone out there know of a polymer based reagent that will work for mouse antibodies on rat tissues? Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 ------------------------------ Message: 8 Date: Sat, 13 Oct 2007 06:46:17 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Keeping frozen mouse aorta on slides To: "Randolph-Habecker, Julie" , histonet@lists.utsouthwestern.edu Message-ID: <260570.30359.qm@web61212.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Julie: The collagen nature of aortas make them more difficult to adhere. I think that you will need a "mechanical barrier" to restrain the sections. Try doing the whole procedure with the slides flat and the sections covered by a very fine plastic mesh that will hold the sections down by its weight. The mesh will allow all the reagents to act. The only problem will be when lifting the mesh that the sections may stick to it. The procedure could also be done through a piece of filter paper, but it is more likely to peel the sections than with the mesh. Just a thought, you may try 1 or 2 sections to see what happens. Ren J. "Randolph-Habecker, Julie" wrote: Folks, I need your suggestions! I am working with an investigator who wants my lab to stain unfixed, OCT embedded mouse aortas. Unfortunately, they have already cut all of the tissue so we do not have a choice of slides. They were cut at 6 microns on Star frost slides and stored at -80C for a few months. When we received them, we took a few slides out, let them air dry at room temperature for 24 hours, put them in a slides rack, soaked them in room temperature PBS for 5 minutes and fixed them with 10% NBF for 10 minutes. These steps are done without agitation. This procedure is compatible with our staining protocol and normally works fine when we use Super frost or Gold Plus slides from Erie. However, in this case, the tissue is floating off the slides in both the PBS and the NBF stage. I have also tried to do these steps with the slides flat and get the same results. Any suggestion on ways to "glue" already cut frozen sections to a charged slide in ways that are compatible with x-gal and IHC staining? Thanks in advance for your help!!!! Julie Julie Randolph-Habecker, Ph.D. Staff Scientist - Director Experimental Histopathology Shared Resource Fred Hutchinson Cancer Research Center 1100 Fairview Ave, N. M5-A803 Seattle WA 98109-1024 206-667-6119 jhabecke@fhcrc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Check out the hottest 2008 models today at Yahoo! Autos. ------------------------------ Message: 9 Date: Sat, 13 Oct 2007 07:14:36 -0700 (PDT) From: Paula Pierce Subject: [Histonet] Keeping frozen mouse aorta on slides To: Histonet Message-ID: <220369.5150.qm@web50102.mail.re2.yahoo.com> Content-Type: text/plain; charset=us-ascii Try going right to formalin after air drying, instead of PBS. Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 630 N. Broadway Moore, OK 73160 405-570-6679 405-759-3953 contact@excaliburpathology.com ------------------------------ Message: 10 Date: Sat, 13 Oct 2007 07:16:16 -0700 (PDT) From: Paula Pierce Subject: [Histonet] polymer based IHC reagent for mouse antibodies on rat tissue To: Histonet Message-ID: <122947.8101.qm@web50106.mail.re2.yahoo.com> Content-Type: text/plain; charset=us-ascii Mouse ImmPress from Vector Labs. Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 630 N. Broadway Moore, OK 73160 405-570-6679 405-759-3953 contact@excaliburpathology.com ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 47, Issue 15 **************************************** From MMargiotta <@t> bmhmc.org Wed Oct 17 10:07:26 2007 From: MMargiotta <@t> bmhmc.org (Margiotta, Michele) Date: Wed Oct 17 10:07:31 2007 Subject: [Histonet] H&E problem Message-ID: <922CE5B88F398948B4076A9A4340E7AF036AF619@bmh_exchange.bmhmc.org> Hi All, We are having a problem with our H&E stain. The pathologist is seeing circular areas on the tissues that are not staining with the hematoxylin(Harris), but they are picking up the Eosin. We are changing the first xylenes and alcohols and hoping that might help. Any idea why this might be happening? Thanks! Michele Margiotta BMHMC Histology Supervisor 631-654-7192 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. From JWEEMS <@t> sjha.org Wed Oct 17 10:14:41 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Oct 17 10:14:59 2007 Subject: [Histonet] H&E problem In-Reply-To: <922CE5B88F398948B4076A9A4340E7AF036AF619@bmh_exchange.bmhmc.org> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F3F4C@sjhaexc02.sjha.org> I'd be sure the slides are completely deparaffinized. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Margiotta, Michele Sent: Wednesday, October 17, 2007 11:07 AM To: histonet@pathology.swmed.edu Subject: [Histonet] H&E problem Hi All, We are having a problem with our H&E stain. The pathologist is seeing circular areas on the tissues that are not staining with the hematoxylin(Harris), but they are picking up the Eosin. We are changing the first xylenes and alcohols and hoping that might help. Any idea why this might be happening? Thanks! Michele Margiotta BMHMC Histology Supervisor 631-654-7192 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From TMcNemar <@t> lmhealth.org Wed Oct 17 10:15:10 2007 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Wed Oct 17 10:15:12 2007 Subject: [Histonet] FUNGI CONTROL BLOCK In-Reply-To: Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F4DC@lmhsmail.lmhealth.org> I sent some out to someone not so long ago so I don't have much left but... You can make your own pretty easily. I streaked some bread mold onto a blood agar plate and let it grow (at room temp). Cut up the agar into cassette-sized pieces (wrapped mine in papers before putting them into the cassettes) and run them through your tissue processor. Loaded with hyphae... beautiful PAS and GMS. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Diana McCaig Sent: Tuesday, October 16, 2007 3:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FUNGI CONTROL BLOCK Does someone have a good fungus control block that demonstrates hyphae? Would be truly appreciated Diana McCaig Histology Lab Chatham Kent Health Alliance Chatham. Ontario N8A 5E1 519-352-6401 (6604) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Oct 17 10:25:44 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Oct 17 10:25:51 2007 Subject: [Histonet] H&E problem In-Reply-To: <922CE5B88F398948B4076A9A4340E7AF036AF619@bmh_exchange.bmhmc.org> Message-ID: <456363.93636.qm@web61219.mail.yahoo.com> Usually that artifact is due to incomplete dewaxing. Make sure your dewaxing reagent is fresh and the time adequate. Ren? J. "Margiotta, Michele" wrote: Hi All, We are having a problem with our H&E stain. The pathologist is seeing circular areas on the tissues that are not staining with the hematoxylin(Harris), but they are picking up the Eosin. We are changing the first xylenes and alcohols and hoping that might help. Any idea why this might be happening? Thanks! Michele Margiotta BMHMC Histology Supervisor 631-654-7192 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From schaundrawalton <@t> yahoo.com Wed Oct 17 10:26:18 2007 From: schaundrawalton <@t> yahoo.com (Schaundra Walton) Date: Wed Oct 17 10:26:21 2007 Subject: [Histonet] Re:Formalin Message-ID: <17189.91014.qm@web58906.mail.re1.yahoo.com> We neutralize our formalin and then dump down the drain. We had been using Formalex, but are in the process of switching to Surgipath's D-Formalizer. D-formalizer is a powder that you add to the formalin. The pH remains neutral and there is a test kit you can get to be sure that the formalin has been neutralized. Its easy to use, cost effective, and only takes about 15-30 minutes to work. It's great. Hope this helps. Schaundra Walton BS HTL(ASCP) Swedish American Hospital 1401 E. State St. Rockford, IL 61104 __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From vazquezr <@t> ohsu.edu Wed Oct 17 10:40:15 2007 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Wed Oct 17 10:40:41 2007 Subject: [Histonet] frozen sections Message-ID: Joyce, How much baby powder do you use, per bottle of OCT? Thanks Robyn >>> "Weems, Joyce" 10/16/2007 12:58 PM >>> Please the tissue in baby powder and it will eliminate the air bubble. You can also add baby powder to the OCT and it will give it more "body" for holding the tissue correctly for freezing. I don't remember where I heard this or learned it... I just know it works! Hope it works for you, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Atoska Gentry Sent: Tuesday, October 16, 2007 3:55 PM To: Histonet Subject: [Histonet] frozen sections hello, has anyone observed empty space surrounding tissue frozen in O.C.T. or similar tissue freezing medium after snap freezing? Upon sectioning samples shipped previously snap frozen to one of our researchers I noticed space surrounding various areas of the tissue. Does anyone know what probably caused this? Could this be a factor in the generation of horrible sections? I suspect the tissue may be moving when the blade touches it. Is there a know remedy that will not damage the tissue? Has anyone ever added O.C.T. to already frozen tissues? Any assistance will be much appreciated. Atoska -- Atoska S. Gentry, B.S., HT(ASCP) Research Assistant IV Scott-Ritchey RSCH Center College of Vet. Med Auburn, AL 36849 PH (334) 844-5579 FAX (334) 844-5850 email: gentras@vetmed.auburn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From JWEEMS <@t> sjha.org Wed Oct 17 10:43:04 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Oct 17 10:43:33 2007 Subject: [Histonet] frozen sections In-Reply-To: Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F3F4E@sjhaexc02.sjha.org> I just mixed it as I needed it and added enough to make a thick paste. Sorry I was not more scientific! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: Robyn Vazquez [mailto:vazquezr@ohsu.edu] Sent: Wednesday, October 17, 2007 11:40 AM To: histonet@pathology.swmed.edu; Weems, Joyce; gentras@vetmed.auburn.edu Subject: RE: [Histonet] frozen sections Joyce, How much baby powder do you use, per bottle of OCT? Thanks Robyn >>> "Weems, Joyce" 10/16/2007 12:58 PM >>> Please the tissue in baby powder and it will eliminate the air bubble. You can also add baby powder to the OCT and it will give it more "body" for holding the tissue correctly for freezing. I don't remember where I heard this or learned it... I just know it works! Hope it works for you, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Atoska Gentry Sent: Tuesday, October 16, 2007 3:55 PM To: Histonet Subject: [Histonet] frozen sections hello, has anyone observed empty space surrounding tissue frozen in O.C.T. or similar tissue freezing medium after snap freezing? Upon sectioning samples shipped previously snap frozen to one of our researchers I noticed space surrounding various areas of the tissue. Does anyone know what probably caused this? Could this be a factor in the generation of horrible sections? I suspect the tissue may be moving when the blade touches it. Is there a know remedy that will not damage the tissue? Has anyone ever added O.C.T. to already frozen tissues? Any assistance will be much appreciated. Atoska -- Atoska S. Gentry, B.S., HT(ASCP) Research Assistant IV Scott-Ritchey RSCH Center College of Vet. Med Auburn, AL 36849 PH (334) 844-5579 FAX (334) 844-5850 email: gentras@vetmed.auburn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From jennifer.l.hofecker <@t> Vanderbilt.Edu Wed Oct 17 11:22:29 2007 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Wed Oct 17 11:22:33 2007 Subject: [Histonet] FUNGI CONTROL BLOCK In-Reply-To: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F4DC@lmhsmail.lmhealth.org> Message-ID: <898D946569A27444B65667A49C074052013D73AA@mailbe06.mc.vanderbilt.edu> Tom, Thanks for passing along the recipe for all of us. I would like to make a plea to anyone who might be "making" some control blocks or who may have some around: The NSH Tissue Control Bank is currently OUT of fungus controls. Would you please consider sending a few our way? I can give you information for mailing. That being said, if anybody comes up with a bunch of good control blocks (for any stain) please consider donating to the bank. If you are a MEMBER of NSH and are in need of a block, let us know. We are always in need of donations of control material. The bank can only exist if those of us who "take" from it "give back" when we can. Thanks in advance for any/all donations. Jennifer L. Hofecker, HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph. (615)343-0083 fax. (615)343-7089 NSH Quality Control Committee Chair -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Wednesday, October 17, 2007 10:15 AM To: Diana McCaig; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] FUNGI CONTROL BLOCK I sent some out to someone not so long ago so I don't have much left but... You can make your own pretty easily. I streaked some bread mold onto a blood agar plate and let it grow (at room temp). Cut up the agar into cassette-sized pieces (wrapped mine in papers before putting them into the cassettes) and run them through your tissue processor. Loaded with hyphae... beautiful PAS and GMS. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Diana McCaig Sent: Tuesday, October 16, 2007 3:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FUNGI CONTROL BLOCK Does someone have a good fungus control block that demonstrates hyphae? Would be truly appreciated Diana McCaig Histology Lab Chatham Kent Health Alliance Chatham. Ontario N8A 5E1 519-352-6401 (6604) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcline <@t> wchsys.org Wed Oct 17 12:18:22 2007 From: jcline <@t> wchsys.org (Joyce Cline) Date: Wed Oct 17 12:18:30 2007 Subject: [Histonet] Slide labeling pen In-Reply-To: Message-ID: <002c01c810e1$b8a1da00$1d2a14ac@wchsys.org> Hi Patti, I received my sample from Mercedes Medical. I agree the pens are great! Joyce Cline ________________________________________________________________________ Hi all. I need some help determining the manufacturer of a slide labeling pen. I received a free sample, and now can't remember where it came from (must be the fumes attacking my brain). The pen is labeled as KP Marker Plus. On our slides it has held up well to deparaffinization reagents, heat pretreatment, etc... Thanks for the help. I'm much chagrined having to post this. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------ - This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From asachau <@t> titanmed.com Wed Oct 17 12:30:05 2007 From: asachau <@t> titanmed.com (April Sachau) Date: Wed Oct 17 12:32:18 2007 Subject: [Histonet] Histology Position $$$ In-Reply-To: <002c01c810e1$b8a1da00$1d2a14ac@wchsys.org> Message-ID: <7E3ACD48BA6E26408F3188FBF08693F7D45AE6@titansbs1.corp.titanmed.com> Hello Everyone! I have a regular Histology position available currently in Minnesota. It is a dayshift position. The pay package will include $26.00/hour; you will be given a $210.00 tax-free per diem weekly; Flight and rental car included (otherwise mileage $.365 per mile paid and a weekly car allowance of $150) Fully furnished housing provided. This is a 13 week assignment with the possibility of an extension. Any interested individuals, please call me at 866-332-9600 ext 1023 or email me at asachau@titanmed.com. Thanks! Have a wonderful day! April Sachau Titan Medical Group Staff Supervisor Phone (866) 332-9600 Ext. 1023 Fax (402) 332-5181 asachau@titanmed.com see us on the web at www.titanmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Wednesday, October 17, 2007 12:18 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide labeling pen Hi Patti, I received my sample from Mercedes Medical. I agree the pens are great! Joyce Cline ________________________________________________________________________ Hi all. I need some help determining the manufacturer of a slide labeling pen. I received a free sample, and now can't remember where it came from (must be the fumes attacking my brain). The pen is labeled as KP Marker Plus. On our slides it has held up well to deparaffinization reagents, heat pretreatment, etc... Thanks for the help. I'm much chagrined having to post this. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------ - This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Maria.Mejia <@t> ucsf.edu Wed Oct 17 15:42:00 2007 From: Maria.Mejia <@t> ucsf.edu (Mejia, Maria) Date: Wed Oct 17 15:42:12 2007 Subject: [Histonet] alternative IHC marker other than GFAP for astrocytes! Message-ID: <6CF686BD6F24A546B85B24FE3B97864701B78F82@EXVS06.net.ucsf.edu> Hello, I wanting to do fluorescent immunostaining on fixed 40um free-floating primate sections & I'm looking for a marker other than GFAP! Can anyone recommend another marker for astrocytes!!!! Regards Maria Bartola Mejia Department of Neurosurgery UCSF SF, CA Lab: 415-514-2954 From TeresaJHarris <@t> msn.com Wed Oct 17 18:49:44 2007 From: TeresaJHarris <@t> msn.com (TeresaJHarris@msn.com) Date: Wed Oct 17 18:49:58 2007 Subject: [Histonet] Slide labeling pen References: <46D38D90616923469B1C86663FC1327402466500@mailserver.mercedesmedical.com> Message-ID: It is a great pen. I highly recommend it. ----- Original Message ----- From: Dave Johnson To: Liz Chlipala ; Patti Loykasek ; histonet Sent: Tuesday, October 16, 2007 2:03 PM Subject: RE: [Histonet] Slide labeling pen Yes, it is indeed Mercedes Medical Give us a call. 800-331-2716 Dave Johnson -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Tuesday, October 16, 2007 2:53 PM To: Patti Loykasek; histonet Subject: RE: [Histonet] Slide labeling pen I thinks it mercedes medical Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti Loykasek Sent: Tuesday, October 16, 2007 12:45 PM To: histonet Subject: [Histonet] Slide labeling pen Hi all. I need some help determining the manufacturer of a slide labeling pen. I received a free sample, and now can't remember where it came from (must be the fumes attacking my brain). The pen is labeled as KP Marker Plus. On our slides it has held up well to deparaffinization reagents, heat pretreatment, etc... Thanks for the help. I'm much chagrined having to post this. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------ - This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pvalente <@t> sbcglobal.net Wed Oct 17 22:49:56 2007 From: pvalente <@t> sbcglobal.net (Patricia Valente) Date: Wed Oct 17 22:50:01 2007 Subject: [Histonet] wax cracks along edge of biopsies Message-ID: <72778.86622.qm@web81714.mail.mud.yahoo.com> Any suggestions about the cause of blocks cracking along the edge of biopsies (happens with some of our prostate biopsies about 14-20 mm long) We have tried to eliminate following factors the temp of the cold plate (-5 C) Care in removing blocks from molds biopsies getting colder before embedding- therefore wax not evenly impregnated Seems to happen along one side of the biopsy causing splitting and or creases when sectioning sometimes only a couple of blocks effected sometimes an irritating amount. usually solved when re-embedded What technique can we change to eliminate problem other details- processing looks OK, cores are not hard, wax in use is polyfin Thanks From Megan.Clarke <@t> hnehealth.nsw.gov.au Thu Oct 18 02:03:18 2007 From: Megan.Clarke <@t> hnehealth.nsw.gov.au (Megan Clarke) Date: Thu Oct 18 02:04:33 2007 Subject: [Histonet] thanks re C9 Message-ID: <471791D6020000250001A5CA@domainatrix.HAHS.HEALTH.NSW.GOV.AU> Hi to the great detectives in Histonet world! We look they find; we querie, they resolve!!! Marvellous! Thanks a stack for the help as always. Zenobia Haffajee Newcastle Australia From christina_bisgaard <@t> yahoo.dk Thu Oct 18 02:13:46 2007 From: christina_bisgaard <@t> yahoo.dk (christina bisgaard) Date: Thu Oct 18 02:13:52 2007 Subject: SV: RE: [Histonet] Laser Capture Microdissection and thick rat brainsections In-Reply-To: Message-ID: <507091.30643.qm@web54406.mail.yahoo.com> Hi Bilqees, The reason I work with native rat brain sections is that formaldehyde fixation cross links the proteins in the tissue, resulting in a "false" 2D protein pattern. So I guess fixing in 37% formaldehyde is not compatible to my protocol?? Christina "Bhatti, Bilqees" skrev: Hi Christina, Try to bring brain sections to room temperature first, fix in 37 % formaldehyde for 5-10 minutes. These are thick sections, rinse in distilled water gently for 3-5 minutes. Then proceed with your protocol. Are you using 75% alcohol for fixation? You can skip this step when you fix in formaldehyde. Good Luck! Bhatti, B Baylor College of Medicine Center for Comparartive Medicine Houston, Texas W # 713-798-6592 U.S.A -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of christina bisgaard Sent: Wednesday, October 17, 2007 6:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Laser Capture Microdissection and thick rat brainsections Dear Histonetters I'm in urgent need of help Does anyone out there have experience with laser capture micro-dissection (Arcturus, Veritas) and thick (60-80?) rat brain section? I have problems collecting more than one Dentate Gyrus (DG) subregion from the hippocampal area from rat brain sections onto the macro cap. I have tried both PEN membrane glass slides and FRAME slides. Could this be due to lack of dehydration? When staining/dehydrating 60? horizontal sections, I observe severe horizontal tearing of the sections if left for more than 30 sec in each dehydration step. If put in Xylene, they are totally broken. Even at 30 sec we sometimes see "blackness" of parts of the sections. My staining protocol is: (using this protocol we don't see much horizontal tearing) 1) Directly from -80oC to 75% EtOH, 30sec 2) dH2O, 30sec 3) Stain, Toluidin Blue, 3 min. 4) dH2O, 30sec 5) 50% EtOH, 30sec 6) 75% EtOH, 30sec 7) 96% EtOH, 30 sec 8) 100% EtOH, 30sec I wish to utilize the cap as much as possible (up to 8 DG per cap) in part for economic reasons. Since the downstream process is 2D gels and protein analysis I need as much tissue from each brain as possible which is why I wish to use thick sections to decrease the number of sections to be microdissected. I'm kind of stuck right now and running out of ideas. I will appreciate any help and will provide more information concerning our protocol if needed. Christina Bisgaard, PhD Stud., Center for Psychiatric Research, Denmark --------------------------------- F? en billig laptop. Se Kelkoos gode tilbud her! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- F? en billig laptop. Se Kelkoos gode tilbud her! From Kemlo.Rogerson <@t> waht.swest.nhs.uk Thu Oct 18 02:38:53 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Oct 18 02:39:02 2007 Subject: [Histonet] H&E problem Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222EF7B@wahtntex2.waht.swest.nhs.uk> "Hi All, We are having a problem with our H&E stain. The pathologist is seeing circular areas on the tissues that are not staining with the hematoxylin(Harris), but they are picking up the Eosin. We are changing the first xylenes and alcohols and hoping that might help. Any idea why this might be happening? Thanks! Michele Margiotta BMHMC Histology Supervisor 631-654-7192" Old chestnut this one; answers usually are poor dewaxing, sections lifting and/ or poor fixation. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Biology is the least of what makes someone a mother. --Oprah Winfrey This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From babatundeajibade <@t> yahoo.com Thu Oct 18 04:44:35 2007 From: babatundeajibade <@t> yahoo.com (Babatunde Ajibade) Date: Thu Oct 18 04:44:39 2007 Subject: [Histonet] I NEED CDX2 CONTROL IHC Message-ID: <180572.99124.qm@web37106.mail.mud.yahoo.com> Can antibody tell me the best CDX2 control tissue, I have been try to get control for this antigen, I have tried colon from diferent samples but I have not be able to get good control, its stain faintly. Thank you in advance. Tunde Ajibade. __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From Farnana <@t> nehealth.com Thu Oct 18 07:09:59 2007 From: Farnana <@t> nehealth.com (Amy Farnan) Date: Thu Oct 18 07:10:29 2007 Subject: [Histonet] Ventana Benchmark Message-ID: <471714D6.26ED.00D9.0@nehealth.com> Hi everyone. I am looking for some feedback on the Ventana Benchmark stainer. I have a tech that worked with a Ventana stainer at her previous job and did not care for it. System was to closed for her. I have another tech that has not used the stainer but went to the recent Region II meeting and attended a workshop on evaluating IHC stainers. The Ventana Benchmark was not given favorable reviews. I do know some area hospitals that have it that really like it. Can you give me some pros and cons? Disclaimer: The information in this message is confidential. If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender. From BMolinari <@t> heart.thi.tmc.edu Thu Oct 18 07:51:21 2007 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Thu Oct 18 07:51:25 2007 Subject: [Histonet] mouse diaphrams Message-ID: It is not Friday but I can see the remarks a mile away on this one. I may have posted this before and I apologize if I have. I am trying to freeze some mouse diaphragms. I continue to get artifact (holes).I have tried freezing on dry ice, dry ice/isopentane slurry, isopentane, Isopentane/ methlybutane . I have blotted the tissue to remove excess moisture all to no avail. Any suggestions? Thanks in advance...should be wild time in Denver w/ World Series going on. I understand that on that Monday the W.S. and Monday night football will be happening at the same time right next door to each other, Betsy Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-6812 From doug <@t> ppspath.com Thu Oct 18 08:51:15 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Thu Oct 18 07:51:33 2007 Subject: SPAM-LOW: [Histonet] Ventana Benchmark In-Reply-To: <471714D6.26ED.00D9.0@nehealth.com> Message-ID: Amy, I am afraid that you just opened a BIG can of worms. Anyway I will give you a different viewpoint than the others will. If you have any concerns of technician turn-over or training anyone that has little or no immuno experience then I recommend the Benchmark XT. It is "almost" dummy-proof. You can have a tech running it in about a week. You will pay for the ease of operation though. To if you are short staffed and have techs with little or no immuno experience it is priceless. The other systems are open systems that will give you a more flexibility. They are more difficult to operate and require a bit more immuno experience and theory. If you have a experienced immuno staff and are not concerned about turnover then this is one to consider. Good luck. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Farnan Sent: Thursday, October 18, 2007 7:10 AM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] Ventana Benchmark Hi everyone. I am looking for some feedback on the Ventana Benchmark stainer. I have a tech that worked with a Ventana stainer at her previous job and did not care for it. System was to closed for her. I have another tech that has not used the stainer but went to the recent Region II meeting and attended a workshop on evaluating IHC stainers. The Ventana Benchmark was not given favorable reviews. I do know some area hospitals that have it that really like it. Can you give me some pros and cons? Disclaimer: The information in this message is confidential. If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Thu Oct 18 08:01:01 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Oct 18 08:01:21 2007 Subject: [Histonet] I NEED CDX2 CONTROL IHC In-Reply-To: <180572.99124.qm@web37106.mail.mud.yahoo.com> References: <180572.99124.qm@web37106.mail.mud.yahoo.com> Message-ID: <471720CD0200007700008988@gwmail4.harthosp.org> We use a section of colon with colonic adenocarcinoma that also contains normal epithelium. If you are seeing weak staining you probably are not achieving optimal antigen retrieval. We use BioGenex's mAb at 1:200 following high pH HIER on the Bond Max and obtain excellent immunoreactivity. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> Babatunde Ajibade 10/18/07 5:44 AM >>> Can antibody tell me the best CDX2 control tissue, I have been try to get control for this antigen, I have tried colon from diferent samples but I have not be able to get good control, its stain faintly. Thank you in advance. Tunde Ajibade. __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From alaskagirl1950 <@t> yahoo.com Thu Oct 18 08:02:00 2007 From: alaskagirl1950 <@t> yahoo.com (Patricia Adams) Date: Thu Oct 18 08:02:04 2007 Subject: Fwd: RE: SPAM-LOW: [Histonet] Ventana Benchmark Message-ID: <679292.50279.qm@web52512.mail.re2.yahoo.com> Amy, I agree with Douglas on this, I have used all of the Ventana IHC systems, and been for training on the Dako, which I am using now. I have liked using both types for different reasons. The Benchmark, because everything is done on board, and the Dako because I have control. In the hospital setting the Benchmark was my choice, put the slides on and finish cutting and staining. Now doing research I need to be able to control all things. All things said I like both. Patricia --- Douglas D Deltour wrote: > From: "Douglas D Deltour" > To: "'Amy Farnan'" , > > Date: Thu, 18 Oct 2007 08:51:15 -0500 > Subject: RE: SPAM-LOW: [Histonet] Ventana > Benchmark > CC: > > Amy, > > I am afraid that you just opened a BIG can of > worms. > > Anyway I will give you a different viewpoint > than the others will. > > If you have any concerns of technician > turn-over or training anyone that has > little or no immuno experience then I recommend > the Benchmark XT. It is > "almost" dummy-proof. You can have a tech > running it in about a week. You > will pay for the ease of operation though. To > if you are short staffed and > have techs with little or no immuno experience > it is priceless. > > The other systems are open systems that will > give you a more flexibility. > They are more difficult to operate and require > a bit more immuno experience > and theory. If you have a experienced immuno > staff and are not concerned > about turnover then this is one to consider. > > Good luck. > > Douglas D. Deltour HT(ASCP) > Histology Manager > Professional Pathology Services, PC > One Science Court > Suite 200 > Columbia, SC 29203 > Office (803)252-1913 > Fax (803)254-3262 > Doug@ppspath.com > ***************************************************** > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of > the individual or entity to > which it is addressed and may contain > information that is privileged, > confidential and exempt from disclosure under > applicable law. If the reader > of this message is not the intended recipient, > you are hereby notified that > any dissemination, distribution, or copying of > this communication is > strictly prohibited by law. If you have > received this communication in > error, please notify me immediately. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Amy Farnan > Sent: Thursday, October 18, 2007 7:10 AM > To: histonet@lists.utsouthwestern.edu > Subject: SPAM-LOW: [Histonet] Ventana Benchmark > > Hi everyone. I am looking for some feedback on > the Ventana Benchmark > stainer. I have a tech that worked with a > Ventana stainer at her previous > job and did not care for it. System was to > closed for her. I have another > tech that has not used the stainer but went to > the recent Region II meeting > and attended a workshop on evaluating IHC > stainers. The Ventana Benchmark > was not given favorable reviews. I do know some > area hospitals that have it > that really like it. Can you give me some pros > and cons? > > Disclaimer: The information in this message is > confidential. If you are not > the intended recipient, do not disclose, copy, > or distribute this message, > and please immediately contact the sender. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From TillRenee <@t> uams.edu Thu Oct 18 08:27:00 2007 From: TillRenee <@t> uams.edu (Till, Renee) Date: Thu Oct 18 08:27:34 2007 Subject: [Histonet] Just curious Message-ID: <11F927674DEBDC43B960809A7403C5D20617CEE8@MAILPED.ad.uams.edu> In terms of histo jobs, what is a special procedures technician? Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72202 Lab (501)364-8504 Office Fax (501)364-3161 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Lynn.Burton <@t> Illinois.gov Thu Oct 18 09:24:48 2007 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Thu Oct 18 09:28:46 2007 Subject: [Histonet] Ventana Benchmark References: <471714D6.26ED.00D9.0@nehealth.com> Message-ID: I came back to Histo after a six year hiatus in virology. Because the state employment agencies are ignorant;y run, I was not allowed to come until Rae Ann retired. She had little or no time to train me at all. I had to come in and train myself on how to run this machine. She did have enough time to show me how to look up protocols and how the machine tells you what is wrong when alarms go off. I have had little or no touble running this machine for the past 11 months. When I have tech support for this company is great. They answer questions over the phone and return calls right away. They even stayed on the phone and walked me through decontamination of the machine one day. Anyone with half an idea how to run a computer can run this stainer. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Amy Farnan Sent: Thu 10/18/2007 7:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana Benchmark Hi everyone. I am looking for some feedback on the Ventana Benchmark stainer. I have a tech that worked with a Ventana stainer at her previous job and did not care for it. System was to closed for her. I have another tech that has not used the stainer but went to the recent Region II meeting and attended a workshop on evaluating IHC stainers. The Ventana Benchmark was not given favorable reviews. I do know some area hospitals that have it that really like it. Can you give me some pros and cons? Disclaimer: The information in this message is confidential. If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janci.Wellborn <@t> stlukes-stl.com Thu Oct 18 09:31:29 2007 From: Janci.Wellborn <@t> stlukes-stl.com (Wellborn, Janci R) Date: Thu Oct 18 09:31:34 2007 Subject: [Histonet] Opne HT Position Message-ID: <42DC3293408E094499C68DDDD45CA08E35CA1F@W3CEXCHANGE2.slh.stlukes.com> Full-time Histology Tech I for St. Luke's Hos Mo. Requirements: Accredited histology program degre of on the job training in Histology. ASCP certification requ Day hours with weekends and holidays as needed. If interested: www jobs@stlukes-stl.com. < Janci R Wellborn, A St. Luke's Hospital 232 South Woods Mill Road Chesterfield, Missouri 63017 (314) 205-6228 janci.wellborn@stlukes-stl.com DISCLAIMER: T p;attachments, contain information which may be&nb sp;confidential, legally privileged, proprietary in&nbs p;nature, or otherwise protected by law from& nbsp;disclosure, and is solely for the use&nb sp;of the intended recipient(s). If you are&n bsp;not the intended recipient, any use, disc losure or copying of this e-mail, including&n bsp;any attachments, is unauthorized and strictly& nbsp;prohibited. If you have received this e- mail in error, please notify us via retu rn e-mail and immediately delete all copies&n bsp;of it from your system. Any opinions ;either expressed or implied in this e-mail&n bsp;and all attachments, are those of its&nbs p;author only, and do not necessarily reflect those of St. Luke's Hospital. < From Dorothy.L.Webb <@t> HealthPartners.Com Thu Oct 18 09:42:03 2007 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Thu Oct 18 09:42:17 2007 Subject: [Histonet] Marking pens In-Reply-To: <01MMMFDHR7NY000IZP@Dino.HealthPartners.Com> Message-ID: <0E394B648E5284478A6CCB78E5AFDA2705635208@hpes1.HealthPartners.int> They are from Mercedes Medical and the order part # is:KLI-KPPEN They come 12 to a box for $30. Subject: [Histonet] Herpes Simplex Virus Controls To: Cc: Linda Sebree , "Colborn, Lisa K." Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello to all... It's a cold, dark, and dreary day in Wisconsin and my husband actually saw snow flakes last week! I'm dreading the winter weather as we approach the holidays. Oh well, on to business... We are in desperate need of some HSV controls and I'm hoping someone out there has a few I could have. I would be willing to swap with some controls that we have in abundance. Thanks in advance, Karen Bauer HT(ASCP) Histology Supervisor Luther Hospital Eau Claire, WI ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. ------------------------------ Message: 2 Date: Tue, 16 Oct 2007 10:54:06 -0700 From: "Mark Elliott" Subject: [Histonet] PAS for frozens To: Message-ID: <4714984E020000D600027301@mail.mrl.ubc.ca> Content-Type: text/plain; charset=US-ASCII Does anyone have a procedure for doing a PAS on frozen tissue?? I assume we need to shorten the times in the various regents, but by how much? Any tips/tricks would be greatly appreciated. We are staining human lung tissue. Thanks Mark in dreary Vancouver BC ***CONFIDENTIALITY NOTICE*** This electronic message is intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. ------------------------------ Message: 3 Date: Tue, 16 Oct 2007 13:57:21 -0400 From: "Satterfield, Marirose" Subject: [Histonet] Formalin To: Message-ID: Content-Type: text/plain; charset="us-ascii" We are going to be switching to Formalin. Our local water department has informed us that we cannot dump the Formalin down the drain. Could I hear some feed back on how you handle your disposal- waste haulers vs. recycling. Thanks Mari Marirose Satterfield Histology Supervisor Mercy Memorial Hospital ------------------------------ Message: 4 Date: Wed, 17 Oct 2007 02:03:41 +0800 From: Shirley PHUA Subject: [Histonet] Shirley Phua is away on course 17-18 October 2007. To: histonet Message-ID: Content-Type: text/plain; charset=US-ASCII I will be out of the office from 17-10-2007 to 18-10-2007. I'll be away on course 17-18 October 2007. I'll be back on 19 October 2007. Pathologists: I will process your requests when I return. If urgent, please forward your email to Henry_Kyaw@hsa.gov.sg ------------------------------ Message: 5 Date: Tue, 16 Oct 2007 11:22:52 -0700 From: Patti Loykasek Subject: [Histonet] Slide labeling pen To: histonet Message-ID: Content-Type: text/plain; charset="US-ASCII" Hi all. I need some help determining the manufacturer of a slide labeling pen. I received a free sample, and now can't remember where it came from (must be the fumes attacking my brain). The pen is labeled as KP Marker Plus. On our slides it has held up well to deparaffinization reagents, heat pretreatment, etc... Thanks for the help. I'm much chagrined having to post this. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. ------------------------------ Message: 6 Date: Tue, 16 Oct 2007 14:34:05 -0400 From: sheila adey Subject: RE: [Histonet] Slide labeling pen To: Patti Loykasek , histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Patti, We love them to. They are from Mercedes Medical. Sheila Adey HT MLTPort Huron HospitalMichigan> Date: Tue, 16 Oct 2007 11:22:52 -0700> From: ploykasek@phenopath.com> To: histonet@pathology.swmed.edu> CC: > Subject: [Histonet] Slide labeling pen> > Hi all. I need some help determining the manufacturer of a slide labeling> pen. I received a free sample, and now can't remember where it came from> (must be the fumes attacking my brain). The pen is labeled as KP Marker> Plus. On our slides it has held up well to deparaffinization reagents, heat> pretreatment, etc...> Thanks for the help. I'm much chagrined having to post this.> > > Patti Loykasek BS, HTL, QIHC> PhenoPath Laboratories> Seattle, WA> > > > -------------------------------------------------------------------------> This e-mail message, including any attachments, is for the sole use of> the intended recipients and may contain privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. If > you are not the intended recipient, please contact the sender by e-mail > _________________________________________________________________ Are you ready for Windows Live Messenger Beta 8.5 ? Get the latest for free today! http://entertainment.sympatico.msn.ca/WindowsLiveMessenger ------------------------------ Message: 7 Date: Tue, 16 Oct 2007 12:53:04 -0600 From: "Liz Chlipala" Subject: RE: [Histonet] Slide labeling pen To: "Patti Loykasek" , "histonet" Message-ID: Content-Type: text/plain; charset="us-ascii" I thinks it mercedes medical Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti Loykasek Sent: Tuesday, October 16, 2007 12:45 PM To: histonet Subject: [Histonet] Slide labeling pen Hi all. I need some help determining the manufacturer of a slide labeling pen. I received a free sample, and now can't remember where it came from (must be the fumes attacking my brain). The pen is labeled as KP Marker Plus. On our slides it has held up well to deparaffinization reagents, heat pretreatment, etc... Thanks for the help. I'm much chagrined having to post this. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------ - This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Tue, 16 Oct 2007 15:03:31 -0400 From: "Dave Johnson" Subject: RE: [Histonet] Slide labeling pen To: "Liz Chlipala" , "Patti Loykasek" , "histonet" Message-ID: <46D38D90616923469B1C86663FC1327402466500@mailserver.mercedesmedical.com> Content-Type: text/plain; charset="us-ascii" Yes, it is indeed Mercedes Medical Give us a call. 800-331-2716 Dave Johnson -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Tuesday, October 16, 2007 2:53 PM To: Patti Loykasek; histonet Subject: RE: [Histonet] Slide labeling pen I thinks it mercedes medical Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti Loykasek Sent: Tuesday, October 16, 2007 12:45 PM To: histonet Subject: [Histonet] Slide labeling pen Hi all. I need some help determining the manufacturer of a slide labeling pen. I received a free sample, and now can't remember where it came from (must be the fumes attacking my brain). The pen is labeled as KP Marker Plus. On our slides it has held up well to deparaffinization reagents, heat pretreatment, etc... Thanks for the help. I'm much chagrined having to post this. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------ - This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Tue, 16 Oct 2007 15:03:47 -0400 From: "Diana McCaig" Subject: [Histonet] FUNGI CONTROL BLOCK To: Message-ID: Content-Type: text/plain; charset="us-ascii" Does someone have a good fungus control block that demonstrates hyphae? Would be truly appreciated Diana McCaig Histology Lab Chatham Kent Health Alliance Chatham. Ontario N8A 5E1 519-352-6401 (6604) ------------------------------ Message: 10 Date: Tue, 16 Oct 2007 14:54:39 -0500 From: Atoska Gentry Subject: [Histonet] frozen sections To: Histonet Message-ID: <471516FF.8000108@vetmed.auburn.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed hello, has anyone observed empty space surrounding tissue frozen in O.C.T. or similar tissue freezing medium after snap freezing? Upon sectioning samples shipped previously snap frozen to one of our researchers I noticed space surrounding various areas of the tissue. Does anyone know what probably caused this? Could this be a factor in the generation of horrible sections? I suspect the tissue may be moving when the blade touches it. Is there a know remedy that will not damage the tissue? Has anyone ever added O.C.T. to already frozen tissues? Any assistance will be much appreciated. Atoska -- Atoska S. Gentry, B.S., HT(ASCP) Research Assistant IV Scott-Ritchey RSCH Center College of Vet. Med Auburn, AL 36849 PH (334) 844-5579 FAX (334) 844-5850 email: gentras@vetmed.auburn.edu ------------------------------ Message: 11 Date: Tue, 16 Oct 2007 15:58:01 -0400 From: "Weems, Joyce" Subject: RE: [Histonet] frozen sections To: "Atoska Gentry" , "Histonet" Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F3F38@sjhaexc02.sjha.org> Content-Type: text/plain; charset="utf-8" Please the tissue in baby powder and it will eliminate the air bubble. You can also add baby powder to the OCT and it will give it more "body" for holding the tissue correctly for freezing. I don't remember where I heard this or learned it... I just know it works! Hope it works for you, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Atoska Gentry Sent: Tuesday, October 16, 2007 3:55 PM To: Histonet Subject: [Histonet] frozen sections hello, has anyone observed empty space surrounding tissue frozen in O.C.T. or similar tissue freezing medium after snap freezing? Upon sectioning samples shipped previously snap frozen to one of our researchers I noticed space surrounding various areas of the tissue. Does anyone know what probably caused this? Could this be a factor in the generation of horrible sections? I suspect the tissue may be moving when the blade touches it. Is there a know remedy that will not damage the tissue? Has anyone ever added O.C.T. to already frozen tissues? Any assistance will be much appreciated. Atoska -- Atoska S. Gentry, B.S., HT(ASCP) Research Assistant IV Scott-Ritchey RSCH Center College of Vet. Med Auburn, AL 36849 PH (334) 844-5579 FAX (334) 844-5850 email: gentras@vetmed.auburn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. ------------------------------ Message: 12 Date: Tue, 16 Oct 2007 13:34:22 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] frozen sections To: Atoska Gentry , Histonet Message-ID: <846676.2416.qm@web61211.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Yes, if there is a space beyween the OCT and the tissue, you can "refill" it and freeze it again. Ren? J. Atoska Gentry wrote: hello, has anyone observed empty space surrounding tissue frozen in O.C.T. or similar tissue freezing medium after snap freezing? Upon sectioning samples shipped previously snap frozen to one of our researchers I noticed space surrounding various areas of the tissue. Does anyone know what probably caused this? Could this be a factor in the generation of horrible sections? I suspect the tissue may be moving when the blade touches it. Is there a know remedy that will not damage the tissue? Has anyone ever added O.C.T. to already frozen tissues? Any assistance will be much appreciated. Atoska -- Atoska S. Gentry, B.S., HT(ASCP) Research Assistant IV Scott-Ritchey RSCH Center College of Vet. Med Auburn, AL 36849 PH (334) 844-5579 FAX (334) 844-5850 email: gentras@vetmed.auburn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Catch up on fall's hot new shows on Yahoo! TV. Watch previews, get listings, and more! ------------------------------ Message: 13 Date: Tue, 16 Oct 2007 16:39:04 -0400 From: Cheryl Cross Subject: [Histonet] crazy immuno question - can you restain slides? To: histonet Message-ID: <009D9AB1-E76A-45DD-83F1-3ABE7BEE760D@aol.com> Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed hi everybody - I'm going back through some of my earlier attempts at immuno for cleaved caspase 3; i have a beautiful lesion i ruined :) on dilutions. is there anyway i can use my newer, better protocol on this previously stained slide? seems such a waste :) thanks for any input! CC Cheryl Cross, DVM, Dipl. ACVP Researcher University Corporation for Atmospheric Research College of Veterinary Medicine University of Tennessee Department of Pathology 2407 River Drive, Room A201 Knoxville, TN 37996-4542 (423) 967-2724 fax: 865-974-5616 ccross@ucar.edu ------------------------------ Message: 14 Date: Tue, 16 Oct 2007 13:58:04 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] crazy immuno question - can you restain slides? To: Cheryl Cross , histonet Message-ID: <474958.73016.qm@web61213.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 If the section already has DAB (as it probably does), you cannot eliminate it. Ren? J. Cheryl Cross wrote: hi everybody - I'm going back through some of my earlier attempts at immuno for cleaved caspase 3; i have a beautiful lesion i ruined :) on dilutions. is there anyway i can use my newer, better protocol on this previously stained slide? seems such a waste :) thanks for any input! CC Cheryl Cross, DVM, Dipl. ACVP Researcher University Corporation for Atmospheric Research College of Veterinary Medicine University of Tennessee Department of Pathology 2407 River Drive, Room A201 Knoxville, TN 37996-4542 (423) 967-2724 fax: 865-974-5616 ccross@ucar.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Need a vacation? Get great deals to amazing places on Yahoo! Travel. ------------------------------ Message: 15 Date: Tue, 16 Oct 2007 17:12:20 -0400 From: Cheryl Cross Subject: Re: [Histonet] crazy immuno question - can you restain slides? To: Rene J Buesa Cc: histonet Message-ID: <980C596F-9F85-4078-B277-D9A8844CB217@aol.com> Content-Type: text/plain; charset=ISO-8859-1; delsp=yes; format=flowed i see - thanks Bonnie and Rene for your input... The old trial sections have not only a lower dilution that didn't work, but a completely different antibody. the rest of the protocol would be the same. it sounds as though if my old sections were understained it may be worth a shot.....but no guarantees :) if i'm understanding you correctly. thanks! CC Cheryl Cross, DVM, Dipl. ACVP Researcher University Corporation for Atmospheric Research College of Veterinary Medicine University of Tennessee Department of Pathology 2407 River Drive, Room A201 Knoxville, TN 37996-4542 (423) 967-2724 fax: 865-974-5616 ccross@ucar.edu On Oct 16, 2007, at 4:58 PM, Rene J Buesa wrote: > If the section already has DAB (as it probably does), you cannot > eliminate it. > Ren? J. > > Cheryl Cross wrote: > hi everybody - > > I'm going back through some of my earlier attempts at immuno for > cleaved caspase 3; i have a beautiful lesion i ruined :) on dilutions. > is there anyway i can use my newer, better protocol on this previously > stained slide? > > seems such a waste :) > > thanks for any input! > > CC > > Cheryl Cross, DVM, Dipl. ACVP > Researcher > University Corporation for Atmospheric Research > College of Veterinary Medicine > University of Tennessee Department of Pathology > 2407 River Drive, Room A201 > Knoxville, TN 37996-4542 > (423) 967-2724 > fax: 865-974-5616 > ccross@ucar.edu > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > Need a vacation? Get great deals to amazing places on Yahoo! Travel. ------------------------------ Message: 16 Date: Tue, 16 Oct 2007 14:46:34 -0700 From: Patti Loykasek Subject: [Histonet] Slide labeling pens To: histonet Message-ID: Content-Type: text/plain; charset="US-ASCII" Thanks to all for the answers to my slide labeling pen question. The KP Marker pens are distributed by Mercedes Medical. Unfortunately, the pens are currently on back order. Call & get your name on the list. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. ------------------------------ Message: 17 Date: Tue, 16 Oct 2007 16:13:59 -0700 From: "Mickie Johnson" Subject: RE: [Histonet] frozen sections To: "'Atoska Gentry'" , "'Histonet'" Message-ID: Content-Type: text/plain; charset="us-ascii" This may be remedied by smearing some OCT to the face of the frozen block. One caution is that this may cause the formation of freezing artifact that looks like small holes next to nuclei or in the center of muscle fibers from the thawing that occurs when adding warm OCT to the frozen block face which then re-freezes causing ice crystals to form. The effect may not go deep in the block though. Good luck. Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Atoska Gentry Sent: Tuesday, October 16, 2007 12:55 PM To: Histonet Subject: [Histonet] frozen sections hello, has anyone observed empty space surrounding tissue frozen in O.C.T. or similar tissue freezing medium after snap freezing? Upon sectioning samples shipped previously snap frozen to one of our researchers I noticed space surrounding various areas of the tissue. Does anyone know what probably caused this? Could this be a factor in the generation of horrible sections? I suspect the tissue may be moving when the blade touches it. Is there a know remedy that will not damage the tissue? Has anyone ever added O.C.T. to already frozen tissues? Any assistance will be much appreciated. Atoska -- Atoska S. Gentry, B.S., HT(ASCP) Research Assistant IV Scott-Ritchey RSCH Center College of Vet. Med Auburn, AL 36849 PH (334) 844-5579 FAX (334) 844-5850 email: gentras@vetmed.auburn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Tue, 16 Oct 2007 17:43:26 -0700 (PDT) From: Malissa Snyder Subject: [Histonet] formalin disposal To: histonet@lists.utsouthwestern.edu Message-ID: <601608.92888.qm@web50208.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 We actually have a powder neutralizer that we put in the jugs of used formalin and then we are able by state and county to pour it down the drain. Maybe, they will let you do the same. --------------------------------- Yahoo! oneSearch: Finally, mobile search that gives answers, not web links. ------------------------------ Message: 19 Date: Wed, 17 Oct 2007 13:08:18 +1000 From: "Megan Clarke" Subject: [Histonet] C9 antibody To: Message-ID: <47160942020000250001A37A@domainatrix.HAHS.HEALTH.NSW.GOV.AU> Content-Type: text/plain; charset=US-ASCII Hi Histonetters We are looking for an antibody to Complement 9 that can be used for Immunohistochemistry stains on formalin fixed paraffin embedded sections. If there is a company out there that supplies this antibody or someone that already uses this antibody, could you please forward this information to us. A distributor in australia would be helpful. Thanking you in anticipation. Zenobia Haffajee HAPS Newcastle Australia ------------------------------ Message: 20 Date: Wed, 17 Oct 2007 10:00:04 +0200 (CEST) From: "Valeria Berno" Subject: [Histonet] cross reaction To: histonet@lists.utsouthwestern.edu Message-ID: <1127.10.251.1.146.1192608004.squirrel@10.251.1.146> Content-Type: text/plain;charset=iso-8859-1 Hi, I am working with a user on neurons culture (embryonic) in order to characterize a list of antibodies for differentiated and undifferentiated markers. Here the problem: either transcription factor (nuclear) and membrane antigens show staining in the nuclei and in the membrane. it seems the antibody sticks on the membrane or enter partially in the nuclei. The protocol is 4%PFA 10min RT, triton 0.3% 10min, bl0cking in Normal goat serum,and washes in TBS-tween0.1%. Any suggestions? Thanks in advance Valeria Berno Valeria, PhD EMBL- Mouse Biology Unit Campus A. Buzzati-Traverso Via Ramarini, 32 00015, Monterotondo Scalo (RM) Italy Tel: +39 06 90091287 Fax: +39 06 90091406 email: valeria.berno@embl.it www.embl.it ------------------------------ Message: 21 Date: Wed, 17 Oct 2007 03:52:40 -0700 From: "Mickie Johnson" Subject: RE: [Histonet] Formalin To: "'Satterfield, Marirose'" , Message-ID: Content-Type: text/plain; charset="us-ascii" Creative Waste Solutions in Portland (888)795-8300 has a filtration system which conserves the buffer salts and so greatly extends the useful life of buffered formalin. Give them a call for more details. Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Satterfield, Marirose Sent: Tuesday, October 16, 2007 10:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin We are going to be switching to Formalin. Our local water department has informed us that we cannot dump the Formalin down the drain. Could I hear some feed back on how you handle your disposal- waste haulers vs. recycling. Thanks Mari Marirose Satterfield Histology Supervisor Mercy Memorial Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 22 Date: Wed, 17 Oct 2007 13:28:13 +0200 (CEST) From: christina bisgaard Subject: [Histonet] Laser Capture Microdissection and thick rat brain sections To: histonet@lists.utsouthwestern.edu Message-ID: <94261.79139.qm@web54410.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Dear Histonetters I'm in urgent need of help Does anyone out there have experience with laser capture micro-dissection (Arcturus, Veritas) and thick (60-80?) rat brain section? I have problems collecting more than one Dentate Gyrus (DG) subregion from the hippocampal area from rat brain sections onto the macro cap. I have tried both PEN membrane glass slides and FRAME slides. Could this be due to lack of dehydration? When staining/dehydrating 60? horizontal sections, I observe severe horizontal tearing of the sections if left for more than 30 sec in each dehydration step. If put in Xylene, they are totally broken. Even at 30 sec we sometimes see "blackness" of parts of the sections. My staining protocol is: (using this protocol we don't see much horizontal tearing) 1) Directly from -80oC to 75% EtOH, 30sec 2) dH2O, 30sec 3) Stain, Toluidin Blue, 3 min. 4) dH2O, 30sec 5) 50% EtOH, 30sec 6) 75% EtOH, 30sec 7) 96% EtOH, 30 sec 8) 100% EtOH, 30sec I wish to utilize the cap as much as possible (up to 8 DG per cap) in part for economic reasons. Since the downstream process is 2D gels and protein analysis I need as much tissue from each brain as possible which is why I wish to use thick sections to decrease the number of sections to be microdissected. I'm kind of stuck right now and running out of ideas. I will appreciate any help and will provide more information concerning our protocol if needed. Christina Bisgaard, PhD Stud., Center for Psychiatric Research, Denmark --------------------------------- F? en billig laptop. Se Kelkoos gode tilbud her! ------------------------------ Message: 23 Date: Wed, 17 Oct 2007 08:05:45 -0400 From: "Green, Kathy" Subject: [Histonet] question about charges To: Message-ID: Content-Type: text/plain; charset="us-ascii" Does anyone have "proof" in regard to the ruling of charging multiple specimens? For example, currently if we get a specimen slip with a uterus, non neoplastic (88307) & an appendix, we can only charge for the "higher" item. Our new director is sure that where he was before they were able to charge for multiple specimens. Our pathologists bill for multiple specimens, but out billing dept. says that we aren't allowed. So if anyone out there does "charge" for multiple specimens like that & have "proof" or something that I can show to billing, I would greatly appreciate it. Kathy Green, HT Manager Histology Laboratory Holy Spirit Hospital 503 N. 21st Street Camp Hill, PA 17011 kgreen@hsh.org (717) 763-2930 Blackberry: (717) 370-1726 Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You ------------------------------ Message: 24 Date: Wed, 17 Oct 2007 08:32:49 -0400 From: Sharon Bledsoe Subject: RE: [Histonet] frozen sections To: "Weems, Joyce" , "Atoska Gentry" , "Histonet" Message-ID: Content-Type: text/plain; charset="us-ascii" ; format="flowed" Joyce, How does the baby powder effect your sample if you have to use birefringence? Talc polarizes, I have to be careful and use non-powdered gloves when I section many of our animal tissue as we are looking for crystals that are identified by birefringence. Do you note on the slide that you have an additive on a frozen section? Sharon Bledsoe At 3:58 PM -0400 10/16/07, Weems, Joyce wrote: >Content-class: urn:content-classes:message >Content-Type: text/plain; > charset="utf-8" > >Please the tissue in baby powder and it will eliminate the air >bubble. You can also add baby powder to the OCT and it will give it >more "body" for holding the tissue correctly for freezing. I don't >remember where I heard this or learned it... I just know it works! >Hope it works for you, j > >Joyce Weems >Pathology Manager >Saint Joseph's Hospital >5665 Peachtree Dunwoody Rd NE >Atlanta, GA 30342 >404-851-7376 - Phone >404-851-7831 - Fax > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Atoska >Gentry >Sent: Tuesday, October 16, 2007 3:55 PM >To: Histonet >Subject: [Histonet] frozen sections > > >hello, has anyone observed empty space surrounding tissue frozen in >O.C.T. or similar tissue freezing medium after snap freezing? Upon >sectioning samples shipped previously snap frozen to one of our >researchers I noticed space surrounding various areas of the tissue. >Does anyone know what probably caused this? Could this be a factor in >the generation of horrible sections? I suspect the tissue may be moving >when the blade touches it. Is there a know remedy that will not damage >the tissue? Has anyone ever added O.C.T. to already frozen tissues? Any >assistance will be much appreciated. Atoska > >-- >Atoska S. Gentry, B.S., HT(ASCP) >Research Assistant IV >Scott-Ritchey RSCH Center >College of Vet. Med >Auburn, AL 36849 >PH (334) 844-5579 >FAX (334) 844-5850 >email: gentras@vetmed.auburn.edu > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >Confidentiality Notice ** The information contained in this message >may be privileged and is confidential information intended for the >use of the addressee listed above. If you are neither the intended >recipient nor the employee or agent responsible for delivering this >message to the intended recipient, you are hereby notified that any >disclosure, copying, distribution or the taking of any action in >reliance on the contents of this information is strictly prohibited. >If you have received this communication in error, please notify us >immediately by replying to the message and deleting it from your >computer. Thank you. Saint Joseph's Health System, Inc. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 25 Date: Wed, 17 Oct 2007 09:02:48 -0400 From: "Weems, Joyce" Subject: RE: [Histonet] question about charges To: "Green, Kathy" , Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F3F41@sjhaexc02.sjha.org> Content-Type: text/plain; charset="utf-8" It's in the CPT code guidelines. There are some specimens that need to be "bundled" for charging, but if you have separate specimens that require separate diagnoses, each specimen gets a separate charge. Yes, you would charge for the uterus - depending on whether it is malignant or not and an 88302 for an incidental appendix. If you have uterus, tubes, and ovaries, there are guidelines about bundling those specimens - sometimes you don't. There are many good resources for billing. Dennis Padget is a good one. He has good instructions and answers for all questions. Our pathologists have subscribed to his services. Good luck. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Green, Kathy Sent: Wednesday, October 17, 2007 8:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] question about charges Does anyone have "proof" in regard to the ruling of charging multiple specimens? For example, currently if we get a specimen slip with a uterus, non neoplastic (88307) & an appendix, we can only charge for the "higher" item. Our new director is sure that where he was before they were able to charge for multiple specimens. Our pathologists bill for multiple specimens, but out billing dept. says that we aren't allowed. So if anyone out there does "charge" for multiple specimens like that & have "proof" or something that I can show to billing, I would greatly appreciate it. Kathy Green, HT Manager Histology Laboratory Holy Spirit Hospital 503 N. 21st Street Camp Hill, PA 17011 kgreen@hsh.org (717) 763-2930 Blackberry: (717) 370-1726 Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. ------------------------------ Message: 26 Date: Wed, 17 Oct 2007 09:05:05 -0400 From: "Weems, Joyce" Subject: RE: [Histonet] Formalin To: , "Satterfield, Marirose" , Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F3F42@sjhaexc02.sjha.org> Content-Type: text/plain; charset="utf-8" We use the Neutralex system from Sakura. They are certified approved with the state of CA. I rest assured that if CA approves, we are safe to us anywhere! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Mickie Johnson Sent: Wednesday, October 17, 2007 6:53 AM To: 'Satterfield, Marirose'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Formalin Creative Waste Solutions in Portland (888)795-8300 has a filtration system which conserves the buffer salts and so greatly extends the useful life of buffered formalin. Give them a call for more details. Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Satterfield, Marirose Sent: Tuesday, October 16, 2007 10:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin We are going to be switching to Formalin. Our local water department has informed us that we cannot dump the Formalin down the drain. Could I hear some feed back on how you handle your disposal- waste haulers vs. recycling. Thanks Mari Marirose Satterfield Histology Supervisor Mercy Memorial Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 47, Issue 19 **************************************** ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From jennifer.l.hofecker <@t> Vanderbilt.Edu Thu Oct 18 10:08:48 2007 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Thu Oct 18 10:08:54 2007 Subject: [Histonet] mouse diaphrams In-Reply-To: Message-ID: <898D946569A27444B65667A49C074052013D73BD@mailbe06.mc.vanderbilt.edu> And the Thermo Party at the Bowling Lanes is Monday night also ... Sorry, no expertise in mouse diaphragms! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Thursday, October 18, 2007 7:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] mouse diaphrams It is not Friday but I can see the remarks a mile away on this one. I may have posted this before and I apologize if I have. I am trying to freeze some mouse diaphragms. I continue to get artifact (holes).I have tried freezing on dry ice, dry ice/isopentane slurry, isopentane, Isopentane/ methlybutane . I have blotted the tissue to remove excess moisture all to no avail. Any suggestions? Thanks in advance...should be wild time in Denver w/ World Series going on. I understand that on that Monday the W.S. and Monday night football will be happening at the same time right next door to each other, Betsy Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-6812 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Thu Oct 18 10:14:40 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Thu Oct 18 10:14:48 2007 Subject: [Histonet] mouse diaphrams In-Reply-To: <898D946569A27444B65667A49C074052013D73BD@mailbe06.mc.vanderbilt.edu> References: <898D946569A27444B65667A49C074052013D73BD@mailbe06.mc.vanderbilt.edu> Message-ID: Well that's one way to keep the rodent population in check, I suppose. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hofecker, Jennifer L Sent: 18 October 2007 16:09 To: Molinari, Betsy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] mouse diaphrams And the Thermo Party at the Bowling Lanes is Monday night also ... Sorry, no expertise in mouse diaphragms! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Thursday, October 18, 2007 7:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] mouse diaphrams It is not Friday but I can see the remarks a mile away on this one. I may have posted this before and I apologize if I have. I am trying to freeze some mouse diaphragms. I continue to get artifact (holes).I have tried freezing on dry ice, dry ice/isopentane slurry, isopentane, Isopentane/ methlybutane . I have blotted the tissue to remove excess moisture all to no avail. Any suggestions? Thanks in advance...should be wild time in Denver w/ World Series going on. I understand that on that Monday the W.S. and Monday night football will be happening at the same time right next door to each other, Betsy Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-6812 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sheila_adey <@t> hotmail.com Thu Oct 18 10:19:46 2007 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Thu Oct 18 10:19:55 2007 Subject: [Histonet] Ventana Benchmark In-Reply-To: References: <471714D6.26ED.00D9.0@nehealth.com> Message-ID: We have a tech here that works at 2 hospitals and the other one has a Ventana. He loves it and says how user friendly it is. He won't even go near our Biogenex i6000. We have had it for 2 years now and still don't have all the bugs worked out. Most frustrating.Sheila Adey HT MLTPort Huron HospitalMichigan> Date: Thu, 18 Oct 2007 09:24:48 -0500> From: Lynn.Burton@Illinois.gov> To: Farnana@nehealth.com; histonet@lists.utsouthwestern.edu> Subject: RE: [Histonet] Ventana Benchmark> CC: > > I came back to Histo after a six year hiatus in virology. Because the state employment agencies are ignorant;y run, I was not allowed to come until Rae Ann retired. She had little or no time to train me at all. I had to come in and train myself on how to run this machine. She did have enough time to show me how to look up protocols and how the machine tells you what is wrong when alarms go off. I have had little or no touble running this machine for the past 11 months. When I have tech support for this company is great. They answer questions over the phone and return calls right away. They even stayed on the phone and walked me through decontamination of the machine one day. Anyone with half an idea how to run a computer can run this stainer.> > ________________________________> > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Amy Farnan> Sent: Thu 10/18/2007 7:09 AM> To: histonet@lists.utsouthwestern.edu> Subject: [Histonet] Ventana Benchmark> > > > Hi everyone. I am looking for some feedback on the Ventana Benchmark stainer. I have a tech that worked with a Ventana stainer at her previous job and did not care for it. System was to closed for her. I have another tech that has not used the stainer but went to the recent Region II meeting and attended a workshop on evaluating IHC stainers. The Ventana Benchmark was not given favorable reviews. I do know some area hospitals that have it that really like it. Can you give me some pros and cons?> > Disclaimer: The information in this message is confidential. If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender.> > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Have fun while connecting on Messenger! Click here to learn more. http://entertainment.sympatico.msn.ca/WindowsLiveMessenger From BMolinari <@t> heart.thi.tmc.edu Thu Oct 18 10:23:07 2007 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Thu Oct 18 10:23:13 2007 Subject: [Histonet] Tiffany Lopez Message-ID: Are you out there? Please contact me. Betsy Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-6812 From sheila_adey <@t> hotmail.com Thu Oct 18 10:26:30 2007 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Thu Oct 18 10:26:36 2007 Subject: [Histonet] Neg controls positive with IHC Message-ID: Hi Everyone, We've been having on going issues with our Negative tissues staining positive. Today the control that had no retrieval was perfect. The slide that was pepsin retrieved was quite positive and the one that was HIER was a little positive. Just a note here, this is the i6000 from Biogenex and the tech support is very inefficient. Very nice people but ..... Any ideas? Thanks in advanceSheila Adey HT MLTPort Huron HospitalMichigan _________________________________________________________________ Have fun while connecting on Messenger! Click here to learn more. http://entertainment.sympatico.msn.ca/WindowsLiveMessenger From jlinda <@t> ces.clemson.edu Thu Oct 18 10:30:32 2007 From: jlinda <@t> ces.clemson.edu (Linda Jenkins) Date: Thu Oct 18 10:30:43 2007 Subject: [Histonet] Career Day in Denver! Message-ID: <6.2.3.4.2.20071018103807.035057d8@mailhost.ces.clemson.edu> We need a few more volunteers for Career Day at the NSH Symposium/Convention. Career Day is in its second year of being and we are planning for another huge success. Basically, we have a hundred or so high school students who will be attending either a morning or afternoon 3 hour, hands-on workshop entitled, "What in the World is Histotechnology". As they travel through the histology galaxy, the students will explore embedding, sectioning, staining, coverslipping, and photography through the microscope. They will also need tour guides to lead them on a scavenger hunt though the exhibit hall. On Monday, October 29, the morning session is from 8:30 - 11:30am and the afternoon session is from 12:00 - 3:00pm. The classrooms will be located in a separate part of the exhibit hall. If you have a free morning or afternoon on Monday and would like to have a really rewarding experience plus obtain one of our special aprons, send Stacey an email at . Mandatory attendance is required at the set-up on Sunday morning at 10:30am - 1:00pm. We had a blast last year in Phoenix. Come join us and see what fun it is to teach your profession to a teenager interested in the field of medicine! Hope to see you in Denver! Stacey Langenberg - Local Career Day Coordinator Linda Jenkins - Career Day Coordinator Linda Jenkins, HT Clemson University Department of Bioengineering 501 Rhodes Research Center Clemson, SC 29634-0905 http://www.ces.clemson.edu/bio/research/histo.html From rjbuesa <@t> yahoo.com Thu Oct 18 10:39:33 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 18 10:39:38 2007 Subject: [Histonet] wax cracks along edge of biopsies In-Reply-To: <72778.86622.qm@web81714.mail.mud.yahoo.com> Message-ID: <607308.72577.qm@web61212.mail.yahoo.com> Some HTs have the costum of opening several cassettes and leaving them on the hot area of the embedding center before embedding. That practice, although adds some productivity to the operation, is not very good and could be that cause of the problem you are having. It will also explain why some present the problem and why others don't. Those staying the more time open, are more likely to have the problem than those embedded shortly after being opened and "in the que" to be embeded. Ren? J. Patricia Valente wrote: Any suggestions about the cause of blocks cracking along the edge of biopsies (happens with some of our prostate biopsies about 14-20 mm long) We have tried to eliminate following factors the temp of the cold plate (-5 C) Care in removing blocks from molds biopsies getting colder before embedding- therefore wax not evenly impregnated Seems to happen along one side of the biopsy causing splitting and or creases when sectioning sometimes only a couple of blocks effected sometimes an irritating amount. usually solved when re-embedded What technique can we change to eliminate problem other details- processing looks OK, cores are not hard, wax in use is polyfin Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From rjbuesa <@t> yahoo.com Thu Oct 18 10:42:23 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 18 10:42:31 2007 Subject: [Histonet] Just curious In-Reply-To: <11F927674DEBDC43B960809A7403C5D20617CEE8@MAILPED.ad.uams.edu> Message-ID: <36195.94644.qm@web61223.mail.yahoo.com> That is an "obscure" term like "histology facilitator" instead of supervisor. There is official recognition of neither. Ren? J. "Till, Renee" wrote: In terms of histo jobs, what is a special procedures technician? Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72202 Lab (501)364-8504 Office Fax (501)364-3161 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From rjbuesa <@t> yahoo.com Thu Oct 18 10:47:21 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 18 10:47:25 2007 Subject: [Histonet] Neg controls positive with IHC In-Reply-To: Message-ID: <154264.26707.qm@web61214.mail.yahoo.com> PERHAPS your problem resides in the antibody titer you are using and which tissue to used to determine it. Variations on the epitope concentration and changes in the target tissue can cause this problem. Ren? J. sheila adey wrote: Hi Everyone, We've been having on going issues with our Negative tissues staining positive. Today the control that had no retrieval was perfect. The slide that was pepsin retrieved was quite positive and the one that was HIER was a little positive. Just a note here, this is the i6000 from Biogenex and the tech support is very inefficient. Very nice people but ..... Any ideas? Thanks in advanceSheila Adey HT MLTPort Huron HospitalMichigan _________________________________________________________________ Have fun while connecting on Messenger! Click here to learn more. http://entertainment.sympatico.msn.ca/WindowsLiveMessenger_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From tahseen <@t> brain.net.pk Thu Oct 18 10:54:48 2007 From: tahseen <@t> brain.net.pk (Tahseen) Date: Thu Oct 18 10:55:03 2007 Subject: [Histonet] H&E problem References: <922CE5B88F398948B4076A9A4340E7AF036AF619@bmh_exchange.bmhmc.org> Message-ID: <004901c8119f$36846e20$8b0b80cb@PDualCore> Dear Michele Margiotta, Just changing the first xylenes. Muhammad Tahseen Histology Supervisor, Shaukat Khanum Memorial Cancer Hospital & Research Centre Lahore,Pakistan. tahseen@brain.net.pk ----- Original Message ----- From: "Margiotta, Michele" To: Sent: Wednesday, October 17, 2007 8:07 PM Subject: [Histonet] H&E problem Hi All, We are having a problem with our H&E stain. The pathologist is seeing circular areas on the tissues that are not staining with the hematoxylin(Harris), but they are picking up the Eosin. We are changing the first xylenes and alcohols and hoping that might help. Any idea why this might be happening? Thanks! Michele Margiotta BMHMC Histology Supervisor 631-654-7192 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sjchtascp <@t> yahoo.com Thu Oct 18 11:05:51 2007 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Thu Oct 18 11:05:56 2007 Subject: [Histonet] Looking for an HT position SO.WI Message-ID: <911736.34214.qm@web38212.mail.mud.yahoo.com> I'm looking for an HT position in So. WI, maybe Madison area or No. Illinois, Rockford area. Thanks, Steve __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From Farnana <@t> nehealth.com Thu Oct 18 11:06:06 2007 From: Farnana <@t> nehealth.com (Amy Farnan) Date: Thu Oct 18 11:06:28 2007 Subject: [Histonet] Ventana Benchmark In-Reply-To: References: <471714D6.26ED.00D9.0@nehealth.com> Message-ID: <47174C2E.26ED.00D9.0@nehealth.com> Wow, I have gotten a lot of great comments. I really appreciate all the feed back. Thanks to all Disclaimer: The information in this message is confidential. If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender. From making <@t> ufl.edu Thu Oct 18 11:17:42 2007 From: making <@t> ufl.edu (MKing) Date: Thu Oct 18 11:18:13 2007 Subject: [Histonet] baby powder freeze aid Message-ID: <47178726.3050209@ufl.edu> Isn't baby powder talc-free these days, cornstarch? Message: 24 Date: Wed, 17 Oct 2007 08:32:49 -0400 From: Sharon Bledsoe Subject: RE: [Histonet] frozen sections To: "Weems, Joyce" , "Atoska Gentry" , "Histonet" Message-ID: Content-Type: text/plain; charset="us-ascii" ; format="flowed" Joyce, How does the baby powder effect your sample if you have to use birefringence? Talc polarizes,... From making <@t> ufl.edu Thu Oct 18 11:24:47 2007 From: making <@t> ufl.edu (MKing) Date: Thu Oct 18 11:25:18 2007 Subject: [Histonet] DAB restain Message-ID: <471788CF.9090905@ufl.edu> I've lightened DAB immersing slides with dilute household bleach (sodium hypochlorite), to the point where it was no longer visible. Try 1:20-1:100 dilution of the 5% commercial liquid. Don't know what happens to it next time you react tissue for DAB, would recommend another substrate or fluorescence, or at least appropriate controls. Mike King -------------- Message: 14 Date: Tue, 16 Oct 2007 13:58:04 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] crazy immuno question - can you restain slides? To: Cheryl Cross , histonet Message-ID: <474958.73016.qm@web61213.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 If the section already has DAB (as it probably does), you cannot eliminate it. Ren? J. Cheryl Cross wrote: hi everybody - I'm going back through some of my earlier attempts at immuno for cleaved caspase 3; i have a beautiful lesion i ruined :) on dilutions. is there anyway i can use my newer, better protocol on this previously stained slide? From jennifer.l.hofecker <@t> Vanderbilt.Edu Thu Oct 18 11:29:18 2007 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Thu Oct 18 11:29:22 2007 Subject: [Histonet] baby powder freeze aid In-Reply-To: <47178726.3050209@ufl.edu> Message-ID: <898D946569A27444B65667A49C074052013D73C2@mailbe06.mc.vanderbilt.edu> I believe you are correct. We use talc (purchased from Fisher) to freeze our muscle biopsies. We have received some samples from outside institutions that were obviously frozen with baby powder (smell powder fresh when you open the package). The samples tend to have some "ice artifact" which is the reason we encourage the use of talc in the first place. If you are looking to reduce freeze artifact (and you are not using fluorescence) go ahead and order the talc from a Scientific product distributor. It's relatively inexpensive. Anybody know if talc protects from "flaming"???? Have a great rest of the week. Jennifer L. Hofecker, HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph. (615)343-0083 fax. (615)343-7089 NSH Quality Control Committee Chair -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MKing Sent: Thursday, October 18, 2007 11:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] baby powder freeze aid Isn't baby powder talc-free these days, cornstarch? Message: 24 Date: Wed, 17 Oct 2007 08:32:49 -0400 From: Sharon Bledsoe Subject: RE: [Histonet] frozen sections To: "Weems, Joyce" , "Atoska Gentry" , "Histonet" Message-ID: Content-Type: text/plain; charset="us-ascii" ; format="flowed" Joyce, How does the baby powder effect your sample if you have to use birefringence? Talc polarizes,... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Thu Oct 18 11:37:16 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Oct 18 11:37:31 2007 Subject: [Histonet] Charging for patient slide send out Message-ID: <4717537C02000077000089BD@gwmail4.harthosp.org> I know we have discussed this before, but how many of you in clinical pathology labs are doing direct patient billing (cash or credit card) for sending out patient slides for second opinions? We are getting so many requests now we cannot keep up, and we certainly can't afford to do this for free. At a minimum, I think the patient should pay the mailing charge. Thank you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From JWEEMS <@t> sjha.org Thu Oct 18 11:45:04 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Thu Oct 18 11:45:19 2007 Subject: [Histonet] Charging for patient slide send out In-Reply-To: <4717537C02000077000089BD@gwmail4.harthosp.org> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F3F7D@sjhaexc02.sjha.org> We have patient pay shipping charge and have facilities that will bill pt ins for second opinion. So many consultants are changing to bill client only that it has really become a problem. j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Richard Cartun Sent: Thursday, October 18, 2007 12:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Charging for patient slide send out I know we have discussed this before, but how many of you in clinical pathology labs are doing direct patient billing (cash or credit card) for sending out patient slides for second opinions? We are getting so many requests now we cannot keep up, and we certainly can't afford to do this for free. At a minimum, I think the patient should pay the mailing charge. Thank you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From rjbuesa <@t> yahoo.com Thu Oct 18 12:04:45 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 18 12:04:51 2007 Subject: [Histonet] DAB restain In-Reply-To: <471788CF.9090905@ufl.edu> Message-ID: <283680.75938.qm@web61217.mail.yahoo.com> Bleach will definitely eliminate DAB, and antigen activity as well! Ren? J. MKing wrote: I've lightened DAB immersing slides with dilute household bleach (sodium hypochlorite), to the point where it was no longer visible. Try 1:20-1:100 dilution of the 5% commercial liquid. Don't know what happens to it next time you react tissue for DAB, would recommend another substrate or fluorescence, or at least appropriate controls. Mike King -------------- Message: 14 Date: Tue, 16 Oct 2007 13:58:04 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] crazy immuno question - can you restain slides? To: Cheryl Cross , histonet Message-ID: <474958.73016.qm@web61213.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 If the section already has DAB (as it probably does), you cannot eliminate it. Ren? J. Cheryl Cross wrote: hi everybody - I'm going back through some of my earlier attempts at immuno for cleaved caspase 3; i have a beautiful lesion i ruined :) on dilutions. is there anyway i can use my newer, better protocol on this previously stained slide? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From Margaret.Perry <@t> sdstate.edu Thu Oct 18 12:11:43 2007 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Thu Oct 18 12:11:48 2007 Subject: [Histonet] sectioning plant seeds Message-ID: We have been asked to section plant seeds that resemble oats. We have tried to run them thru formalin and processed the same as our regular animal tissue. Then we embedded in wax. We cannot get a good section. Has anyone worked with plant seeds? How do you fix and process them. Any help will be greatly appreciated. I believe they want to do ISH on the slides. Thanks. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 From PIXLEYSK <@t> UCMAIL.UC.EDU Thu Oct 18 12:12:08 2007 From: PIXLEYSK <@t> UCMAIL.UC.EDU (Pixley, Sarah (pixleysk)) Date: Thu Oct 18 12:12:17 2007 Subject: [Histonet] DAB restain In-Reply-To: <200710181705.DXH56874@mprelay1.uc.edu> Message-ID: Dear Mike: Beware using bleach and then trying to restain with DAB. We have found that if we even tried to re-use glass containers that had held DAB and that we subsequently bleached to inactivate the DAB, the glass absorbed and retained the bleach and would inactivate our next DAB solution. So the glass slides might retain the bleach and inactivate. Etc. So I agree with the previous person, use another chromagen or fluorescence after bleaching, if it does get rid of any of the staining. Sarah Pixley From LRaff <@t> lab.uropartners.com Thu Oct 18 12:16:55 2007 From: LRaff <@t> lab.uropartners.com (Lester Raff) Date: Thu Oct 18 12:16:59 2007 Subject: [Histonet] Charging for patient slide send out In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F3F7D@sjhaexc02.sjha.org> Message-ID: <5DA1CA5D0B98A84985B545A24423B8220531AC@UPLAB01.uplab.local> We do a lot of send outs for second opinion. We send them all by Fedex. We have a standard form and a standard fee of $35, usually paid in advance by patient credit card. Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Thursday, October 18, 2007 11:45 AM To: Richard Cartun; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Charging for patient slide send out We have patient pay shipping charge and have facilities that will bill pt ins for second opinion. So many consultants are changing to bill client only that it has really become a problem. j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Richard Cartun Sent: Thursday, October 18, 2007 12:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Charging for patient slide send out I know we have discussed this before, but how many of you in clinical pathology labs are doing direct patient billing (cash or credit card) for sending out patient slides for second opinions? We are getting so many requests now we cannot keep up, and we certainly can't afford to do this for free. At a minimum, I think the patient should pay the mailing charge. Thank you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. -- I am using the free version of SPAMfighter for private users. It has removed 2565 spam emails to date. Paying users do not have this message in their emails. Get the free SPAMfighter here: http://www.spamfighter.com/len From Heather.D.Renko <@t> osfhealthcare.org Thu Oct 18 12:18:48 2007 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Thu Oct 18 12:19:03 2007 Subject: [Histonet] RE: world series/monday night football/NSH Message-ID: <40026EDDE64CDA47AB382C52619ACD3C0A09DB6E@pmc-rfd-mx01.intranet.osfnet.org> OK, what sports pub will we all be meeting at? See you in Denver! Heather D. Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 Direct: 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From BWeston <@t> BarbHosp.com Thu Oct 18 12:25:25 2007 From: BWeston <@t> BarbHosp.com (Weston, Bernadette) Date: Thu Oct 18 12:25:32 2007 Subject: [Histonet] screened cassettes Message-ID: Can anyone tell me their criteria for using screened cassettes, what dimensions? All small skin? Do you wrap tiny pieces in filter paper in addition to putting it into a screened cassette? I am concerned if a piece in wrapped as well as put in the screened cassette that the fixative will penetrate. Any thoughts on this subject would be appreciated. Bernadette Weston HT Histology Supervisor Barberton Citizens Hospital 330 615 3980 bweston@barbhosp.com From BMolinari <@t> heart.thi.tmc.edu Thu Oct 18 12:29:37 2007 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Thu Oct 18 12:29:39 2007 Subject: [Histonet] RE: world series/monday night football/NSH In-Reply-To: <40026EDDE64CDA47AB382C52619ACD3C0A09DB6E@pmc-rfd-mx01.intranet.osfnet.org> Message-ID: Well..I may pass...I am a tried and true Red Sox fan and will be sitting on the edge of my seat tonight....hopefully I will be cheering in Denver..otherwise I will be bowling. Betsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Renko, Heather D. Sent: Thursday, October 18, 2007 12:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: world series/monday night football/NSH OK, what sports pub will we all be meeting at? See you in Denver! Heather D. Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 Direct: 815-395-5410 Heather.D.Renko@osfhealthcare.org ======================================================================== ====== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> CDC.GOV Thu Oct 18 12:35:37 2007 From: jqb7 <@t> CDC.GOV (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Thu Oct 18 12:36:06 2007 Subject: [Histonet] RE: world series/monday night football/NSH In-Reply-To: <40026EDDE64CDA47AB382C52619ACD3C0A09DB6E@pmc-rfd-mx01.intranet.osfnet.org> References: <40026EDDE64CDA47AB382C52619ACD3C0A09DB6E@pmc-rfd-mx01.intranet.osfnet.org> Message-ID: <34BB307EFC9A65429BBB49E330675F72045E209A@LTA3VS003.ees.hhs.gov> ANY of them! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Renko, Heather D. Sent: Thursday, October 18, 2007 1:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: world series/monday night football/NSH OK, what sports pub will we all be meeting at? See you in Denver! Heather D. Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 Direct: 815-395-5410 Heather.D.Renko@osfhealthcare.org ======================================================================== ====== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lynne.Bell <@t> hitchcock.org Thu Oct 18 12:47:40 2007 From: Lynne.Bell <@t> hitchcock.org (Bell, Lynne) Date: Thu Oct 18 12:47:45 2007 Subject: [Histonet] RE: world series/monday night football/NSH In-Reply-To: Message-ID: Betsy, I feel your pain - I'm a life long Red Sox fan as well....... Unfortunately, I won't be at the NSH convention. Lynne From RSRICHMOND <@t> aol.com Thu Oct 18 12:50:02 2007 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Thu Oct 18 12:50:05 2007 Subject: [Histonet] Re: baby powder freeze aid Message-ID: Both talc and cornstarch baby powders are still available. I have purchased each of them within the last few weeks - needed it to re-use thick latex "chemotherapy gloves" at one hospital that didn't believe in nitrile rubber gloves for formaldehyde. I bought the talc powder at Wal-Mart, in what seemed obviously a misbranded container. I bought the cornstarch at Walgreen's. Both talc and starch particles are highly birefringent with a polarizer. I've used talc for freezing muscle biopsy specimens - have never tried cornstarch. By the way, don't ever put talc on a baby's butt - some talc products contain asbestos fibers. Use cornstarch. Bob Richmond Samurai Pathologist (and long-ago baby's butt powderer) Knoxville TN From rjbuesa <@t> yahoo.com Thu Oct 18 12:56:01 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 18 12:56:07 2007 Subject: [Histonet] screened cassettes In-Reply-To: Message-ID: <76878.21634.qm@web61221.mail.yahoo.com> If you use a screened cassette, you don't need to wrap them (but you will not feel comfortable doing that until you get use to using the screened cassettes). Ren? J. "Weston, Bernadette" wrote: Can anyone tell me their criteria for using screened cassettes, what dimensions? All small skin? Do you wrap tiny pieces in filter paper in addition to putting it into a screened cassette? I am concerned if a piece in wrapped as well as put in the screened cassette that the fixative will penetrate. Any thoughts on this subject would be appreciated. Bernadette Weston HT Histology Supervisor Barberton Citizens Hospital 330 615 3980 bweston@barbhosp.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From rjbuesa <@t> yahoo.com Thu Oct 18 12:58:32 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 18 12:58:37 2007 Subject: [Histonet] sectioning plant seeds In-Reply-To: Message-ID: <549044.76095.qm@web61225.mail.yahoo.com> You will have to break the surface of the seeds, just a little bit, to allow the chemicals to penetrate and do their wotk during processing. Ren? J. "Perry, Margaret" wrote: We have been asked to section plant seeds that resemble oats. We have tried to run them thru formalin and processed the same as our regular animal tissue. Then we embedded in wax. We cannot get a good section. Has anyone worked with plant seeds? How do you fix and process them. Any help will be greatly appreciated. I believe they want to do ISH on the slides. Thanks. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From pruegg <@t> ihctech.net Thu Oct 18 13:13:12 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Thu Oct 18 13:13:19 2007 Subject: [Histonet] NSH S/C in Denver Message-ID: <008f01c811b2$8c9bc9d0$6601a8c0@Patsy> NSH moved the S/C to Denver and a World Series broke out. World Series games on Oct.27, 28 and 29 are expected to be in Denver (yes it will be crazy downtown where the meeting is taking place, god help us find places to eat and park, etc.) If you want to try and get tickets to a game, they go on sale next Monday Oct. 22 at 10 am online only at www.coloradorockies.com . Hope to see you in Denver, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC 12635 Montview Blvd. Ste.215 Aurora, Colorado 80045 Phone: 720-859-4060 Fax: 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org From erweber <@t> maxhealth.com Thu Oct 18 13:14:05 2007 From: erweber <@t> maxhealth.com (Eric Weber) Date: Thu Oct 18 13:13:42 2007 Subject: [Histonet] Pathologist's Assistant Message-ID: <9D12D4EF30176D4F839EB47F3D0E8436027B8605@exbk2.maxhealth.com> Looking for help, Can anyone explain the requirements for a Pathology Assistant? Thank you. Eric Weber Maxim Healthcare Services Toll Free (877) 263-7823 Toll Free Fax (866) 466-2981 erweber@maxhealth.com From lblazek <@t> digestivespecialists.com Thu Oct 18 13:17:53 2007 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Oct 18 13:15:13 2007 Subject: [Histonet] RE: world series/monday night football/NSH OT In-Reply-To: References: <40026EDDE64CDA47AB382C52619ACD3C0A09DB6E@pmc-rfd-mx01.intranet.osfnet.org> Message-ID: <1F937FB30BDB7C4A9F39F83FEA8D379F3E4CA7@bruexchange1.digestivespecialists.com> I'll be on the edge of my seat tonight too! But I'm tried and true INDIAN fan. GO TRIBE!!! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Thursday, October 18, 2007 1:30 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: world series/monday night football/NSH Well..I may pass...I am a tried and true Red Sox fan and will be sitting on the edge of my seat tonight....hopefully I will be cheering in Denver..otherwise I will be bowling. Betsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Renko, Heather D. Sent: Thursday, October 18, 2007 12:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: world series/monday night football/NSH OK, what sports pub will we all be meeting at? See you in Denver! Heather D. Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 Direct: 815-395-5410 Heather.D.Renko@osfhealthcare.org ======================================================================== ====== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nancy.Warren <@t> nkch.org Thu Oct 18 13:20:26 2007 From: Nancy.Warren <@t> nkch.org (Nancy Warren) Date: Thu Oct 18 13:20:55 2007 Subject: [Histonet] RE: Histonet Digest, Vol 47, Issue 22 In-Reply-To: Message-ID: <77FADC01535C0B4FAC7EC79961E9B73C5E6A4E@Belgian.nkch.org> Regarding #16 patient charges. You can charge per slide. We charge $20 per slide for referrals outside of diagnostic purposes. Research, legal or for someones interest. It must be across the board with no exceptions. It has cut down on the number of requests and re-requests because of lost slides. Some doctors get mad, but stick to your guns and you will get this accomplished and not be going crazy with requests. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, October 18, 2007 12:03 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 47, Issue 22 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: mouse diaphrams (Hofecker, Jennifer L) 2. RE: mouse diaphrams (Edwards, R.E.) 3. RE: Ventana Benchmark (sheila adey) 4. Tiffany Lopez (Molinari, Betsy) 5. Neg controls positive with IHC (sheila adey) 6. Career Day in Denver! (Linda Jenkins) 7. Re: wax cracks along edge of biopsies (Rene J Buesa) 8. Re: Just curious (Rene J Buesa) 9. Re: Neg controls positive with IHC (Rene J Buesa) 10. Re: H&E problem (Tahseen) 11. Looking for an HT position SO.WI (Steven Coakley) 12. RE: Ventana Benchmark (Amy Farnan) 13. baby powder freeze aid (MKing) 14. DAB restain (MKing) 15. RE: baby powder freeze aid (Hofecker, Jennifer L) 16. Charging for patient slide send out (Richard Cartun) 17. RE: Charging for patient slide send out (Weems, Joyce) ---------------------------------------------------------------------- Message: 1 Date: Thu, 18 Oct 2007 10:08:48 -0500 From: "Hofecker, Jennifer L" Subject: RE: [Histonet] mouse diaphrams To: "Molinari, Betsy" , Message-ID: <898D946569A27444B65667A49C074052013D73BD@mailbe06.mc.vanderbilt.edu> Content-Type: text/plain; charset="us-ascii" And the Thermo Party at the Bowling Lanes is Monday night also ... Sorry, no expertise in mouse diaphragms! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Thursday, October 18, 2007 7:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] mouse diaphrams It is not Friday but I can see the remarks a mile away on this one. I may have posted this before and I apologize if I have. I am trying to freeze some mouse diaphragms. I continue to get artifact (holes).I have tried freezing on dry ice, dry ice/isopentane slurry, isopentane, Isopentane/ methlybutane . I have blotted the tissue to remove excess moisture all to no avail. Any suggestions? Thanks in advance...should be wild time in Denver w/ World Series going on. I understand that on that Monday the W.S. and Monday night football will be happening at the same time right next door to each other, Betsy Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-6812 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Thu, 18 Oct 2007 16:14:40 +0100 From: "Edwards, R.E." Subject: RE: [Histonet] mouse diaphrams To: "Hofecker, Jennifer L" , "Molinari, Betsy" , Message-ID: Content-Type: text/plain; charset="US-ASCII" Well that's one way to keep the rodent population in check, I suppose. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hofecker, Jennifer L Sent: 18 October 2007 16:09 To: Molinari, Betsy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] mouse diaphrams And the Thermo Party at the Bowling Lanes is Monday night also ... Sorry, no expertise in mouse diaphragms! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Thursday, October 18, 2007 7:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] mouse diaphrams It is not Friday but I can see the remarks a mile away on this one. I may have posted this before and I apologize if I have. I am trying to freeze some mouse diaphragms. I continue to get artifact (holes).I have tried freezing on dry ice, dry ice/isopentane slurry, isopentane, Isopentane/ methlybutane . I have blotted the tissue to remove excess moisture all to no avail. Any suggestions? Thanks in advance...should be wild time in Denver w/ World Series going on. I understand that on that Monday the W.S. and Monday night football will be happening at the same time right next door to each other, Betsy Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-6812 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Thu, 18 Oct 2007 11:19:46 -0400 From: sheila adey Subject: RE: [Histonet] Ventana Benchmark To: "Burton, Lynn" , Amy Farnan , Message-ID: Content-Type: text/plain; charset="iso-8859-1" We have a tech here that works at 2 hospitals and the other one has a Ventana. He loves it and says how user friendly it is. He won't even go near our Biogenex i6000. We have had it for 2 years now and still don't have all the bugs worked out. Most frustrating.Sheila Adey HT MLTPort Huron HospitalMichigan> Date: Thu, 18 Oct 2007 09:24:48 -0500> From: Lynn.Burton@Illinois.gov> To: Farnana@nehealth.com; histonet@lists.utsouthwestern.edu> Subject: RE: [Histonet] Ventana Benchmark> CC: > > I came back to Histo after a six year hiatus in virology. Because the state employment agencies are ignorant;y run, I was not allowed to come until Rae Ann retired. She had little or no time to train me at all. I had to come in and train myself on how to run this machine. She did have enough time to show me how to look up protocols and how the machine tells you what is wrong when alarms go off. I have had little or no touble running this machine for the past 11 months. When I have tech support for this company is great. They answer questions over the phone and return calls right away. They even stayed on the phone and walked me through decontamination of the machine one day. Anyone with half an idea how to run a computer can run this stainer.> > ________________________________> > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Amy Farnan> Sent: Thu 10/18/2007 7:09 AM> To: histonet@lists.utsouthwestern.edu> Subject: [Histonet] Ventana Benchmark> > > > Hi everyone. I am looking for some feedback on the Ventana Benchmark stainer. I have a tech that worked with a Ventana stainer at her previous job and did not care for it. System was to closed for her. I have another tech that has not used the stainer but went to the recent Region II meeting and attended a workshop on evaluating IHC stainers. The Ventana Benchmark was not given favorable reviews. I do know some area hospitals that have it that really like it. Can you give me some pros and cons?> > Disclaimer: The information in this message is confidential. If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender.> > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Have fun while connecting on Messenger! Click here to learn more. http://entertainment.sympatico.msn.ca/WindowsLiveMessenger ------------------------------ Message: 4 Date: Thu, 18 Oct 2007 10:23:07 -0500 From: "Molinari, Betsy" Subject: [Histonet] Tiffany Lopez To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Are you out there? Please contact me. Betsy Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-6812 ------------------------------ Message: 5 Date: Thu, 18 Oct 2007 11:26:30 -0400 From: sheila adey Subject: [Histonet] Neg controls positive with IHC To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Everyone, We've been having on going issues with our Negative tissues staining positive. Today the control that had no retrieval was perfect. The slide that was pepsin retrieved was quite positive and the one that was HIER was a little positive. Just a note here, this is the i6000 from Biogenex and the tech support is very inefficient. Very nice people but ..... Any ideas? Thanks in advanceSheila Adey HT MLTPort Huron HospitalMichigan _________________________________________________________________ Have fun while connecting on Messenger! Click here to learn more. http://entertainment.sympatico.msn.ca/WindowsLiveMessenger ------------------------------ Message: 6 Date: Thu, 18 Oct 2007 11:30:32 -0400 From: Linda Jenkins Subject: [Histonet] Career Day in Denver! To: histonet@lists.utsouthwestern.edu Message-ID: <6.2.3.4.2.20071018103807.035057d8@mailhost.ces.clemson.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed We need a few more volunteers for Career Day at the NSH Symposium/Convention. Career Day is in its second year of being and we are planning for another huge success. Basically, we have a hundred or so high school students who will be attending either a morning or afternoon 3 hour, hands-on workshop entitled, "What in the World is Histotechnology". As they travel through the histology galaxy, the students will explore embedding, sectioning, staining, coverslipping, and photography through the microscope. They will also need tour guides to lead them on a scavenger hunt though the exhibit hall. On Monday, October 29, the morning session is from 8:30 - 11:30am and the afternoon session is from 12:00 - 3:00pm. The classrooms will be located in a separate part of the exhibit hall. If you have a free morning or afternoon on Monday and would like to have a really rewarding experience plus obtain one of our special aprons, send Stacey an email at . Mandatory attendance is required at the set-up on Sunday morning at 10:30am - 1:00pm. We had a blast last year in Phoenix. Come join us and see what fun it is to teach your profession to a teenager interested in the field of medicine! Hope to see you in Denver! Stacey Langenberg - Local Career Day Coordinator Linda Jenkins - Career Day Coordinator Linda Jenkins, HT Clemson University Department of Bioengineering 501 Rhodes Research Center Clemson, SC 29634-0905 http://www.ces.clemson.edu/bio/research/histo.html ------------------------------ Message: 7 Date: Thu, 18 Oct 2007 08:39:33 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] wax cracks along edge of biopsies To: Patricia Valente , Histonet@lists.utsouthwestern.edu Message-ID: <607308.72577.qm@web61212.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Some HTs have the costum of opening several cassettes and leaving them on the hot area of the embedding center before embedding. That practice, although adds some productivity to the operation, is not very good and could be that cause of the problem you are having. It will also explain why some present the problem and why others don't. Those staying the more time open, are more likely to have the problem than those embedded shortly after being opened and "in the que" to be embeded. Ren? J. Patricia Valente wrote: Any suggestions about the cause of blocks cracking along the edge of biopsies (happens with some of our prostate biopsies about 14-20 mm long) We have tried to eliminate following factors the temp of the cold plate (-5 C) Care in removing blocks from molds biopsies getting colder before embedding- therefore wax not evenly impregnated Seems to happen along one side of the biopsy causing splitting and or creases when sectioning sometimes only a couple of blocks effected sometimes an irritating amount. usually solved when re-embedded What technique can we change to eliminate problem other details- processing looks OK, cores are not hard, wax in use is polyfin Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com ------------------------------ Message: 8 Date: Thu, 18 Oct 2007 08:42:23 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Just curious To: "Till, Renee" , histonet@lists.utsouthwestern.edu Message-ID: <36195.94644.qm@web61223.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 That is an "obscure" term like "histology facilitator" instead of supervisor. There is official recognition of neither. Ren? J. "Till, Renee" wrote: In terms of histo jobs, what is a special procedures technician? Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72202 Lab (501)364-8504 Office Fax (501)364-3161 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com ------------------------------ Message: 9 Date: Thu, 18 Oct 2007 08:47:21 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Neg controls positive with IHC To: sheila adey , "histonet@lists.utsouthwestern.edu" Message-ID: <154264.26707.qm@web61214.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 PERHAPS your problem resides in the antibody titer you are using and which tissue to used to determine it. Variations on the epitope concentration and changes in the target tissue can cause this problem. Ren? J. sheila adey wrote: Hi Everyone, We've been having on going issues with our Negative tissues staining positive. Today the control that had no retrieval was perfect. The slide that was pepsin retrieved was quite positive and the one that was HIER was a little positive. Just a note here, this is the i6000 from Biogenex and the tech support is very inefficient. Very nice people but ..... Any ideas? Thanks in advanceSheila Adey HT MLTPort Huron HospitalMichigan _________________________________________________________________ Have fun while connecting on Messenger! Click here to learn more. http://entertainment.sympatico.msn.ca/WindowsLiveMessenger_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com ------------------------------ Message: 10 Date: Thu, 18 Oct 2007 20:54:48 +0500 From: "Tahseen" Subject: Re: [Histonet] H&E problem To: "Margiotta, Michele" , Message-ID: <004901c8119f$36846e20$8b0b80cb@PDualCore> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Dear Michele Margiotta, Just changing the first xylenes. Muhammad Tahseen Histology Supervisor, Shaukat Khanum Memorial Cancer Hospital & Research Centre Lahore,Pakistan. tahseen@brain.net.pk ----- Original Message ----- From: "Margiotta, Michele" To: Sent: Wednesday, October 17, 2007 8:07 PM Subject: [Histonet] H&E problem Hi All, We are having a problem with our H&E stain. The pathologist is seeing circular areas on the tissues that are not staining with the hematoxylin(Harris), but they are picking up the Eosin. We are changing the first xylenes and alcohols and hoping that might help. Any idea why this might be happening? Thanks! Michele Margiotta BMHMC Histology Supervisor 631-654-7192 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Thu, 18 Oct 2007 09:05:51 -0700 (PDT) From: Steven Coakley Subject: [Histonet] Looking for an HT position SO.WI To: Histonet@lists.utsouthwestern.edu Message-ID: <911736.34214.qm@web38212.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I'm looking for an HT position in So. WI, maybe Madison area or No. Illinois, Rockford area. Thanks, Steve __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com ------------------------------ Message: 12 Date: Thu, 18 Oct 2007 12:06:06 -0400 From: "Amy Farnan" Subject: RE: [Histonet] Ventana Benchmark To: "sheila adey" , "Lynn Burton" , Message-ID: <47174C2E.26ED.00D9.0@nehealth.com> Content-Type: text/plain; charset=US-ASCII Wow, I have gotten a lot of great comments. I really appreciate all the feed back. Thanks to all Disclaimer: The information in this message is confidential. If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender. ------------------------------ Message: 13 Date: Thu, 18 Oct 2007 12:17:42 -0400 From: MKing Subject: [Histonet] baby powder freeze aid To: histonet@lists.utsouthwestern.edu Message-ID: <47178726.3050209@ufl.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Isn't baby powder talc-free these days, cornstarch? Message: 24 Date: Wed, 17 Oct 2007 08:32:49 -0400 From: Sharon Bledsoe Subject: RE: [Histonet] frozen sections To: "Weems, Joyce" , "Atoska Gentry" , "Histonet" Message-ID: Content-Type: text/plain; charset="us-ascii" ; format="flowed" Joyce, How does the baby powder effect your sample if you have to use birefringence? Talc polarizes,... ------------------------------ Message: 14 Date: Thu, 18 Oct 2007 12:24:47 -0400 From: MKing Subject: [Histonet] DAB restain To: histonet@lists.utsouthwestern.edu Message-ID: <471788CF.9090905@ufl.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed I've lightened DAB immersing slides with dilute household bleach (sodium hypochlorite), to the point where it was no longer visible. Try 1:20-1:100 dilution of the 5% commercial liquid. Don't know what happens to it next time you react tissue for DAB, would recommend another substrate or fluorescence, or at least appropriate controls. Mike King -------------- Message: 14 Date: Tue, 16 Oct 2007 13:58:04 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] crazy immuno question - can you restain slides? To: Cheryl Cross , histonet Message-ID: <474958.73016.qm@web61213.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 If the section already has DAB (as it probably does), you cannot eliminate it. Ren? J. Cheryl Cross wrote: hi everybody - I'm going back through some of my earlier attempts at immuno for cleaved caspase 3; i have a beautiful lesion i ruined :) on dilutions. is there anyway i can use my newer, better protocol on this previously stained slide? ------------------------------ Message: 15 Date: Thu, 18 Oct 2007 11:29:18 -0500 From: "Hofecker, Jennifer L" Subject: RE: [Histonet] baby powder freeze aid To: "MKing" , Message-ID: <898D946569A27444B65667A49C074052013D73C2@mailbe06.mc.vanderbilt.edu> Content-Type: text/plain; charset="us-ascii" I believe you are correct. We use talc (purchased from Fisher) to freeze our muscle biopsies. We have received some samples from outside institutions that were obviously frozen with baby powder (smell powder fresh when you open the package). The samples tend to have some "ice artifact" which is the reason we encourage the use of talc in the first place. If you are looking to reduce freeze artifact (and you are not using fluorescence) go ahead and order the talc from a Scientific product distributor. It's relatively inexpensive. Anybody know if talc protects from "flaming"???? Have a great rest of the week. Jennifer L. Hofecker, HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph. (615)343-0083 fax. (615)343-7089 NSH Quality Control Committee Chair -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MKing Sent: Thursday, October 18, 2007 11:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] baby powder freeze aid Isn't baby powder talc-free these days, cornstarch? Message: 24 Date: Wed, 17 Oct 2007 08:32:49 -0400 From: Sharon Bledsoe Subject: RE: [Histonet] frozen sections To: "Weems, Joyce" , "Atoska Gentry" , "Histonet" Message-ID: Content-Type: text/plain; charset="us-ascii" ; format="flowed" Joyce, How does the baby powder effect your sample if you have to use birefringence? Talc polarizes,... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Thu, 18 Oct 2007 12:37:16 -0400 From: "Richard Cartun" Subject: [Histonet] Charging for patient slide send out To: Message-ID: <4717537C02000077000089BD@gwmail4.harthosp.org> Content-Type: text/plain; charset=US-ASCII I know we have discussed this before, but how many of you in clinical pathology labs are doing direct patient billing (cash or credit card) for sending out patient slides for second opinions? We are getting so many requests now we cannot keep up, and we certainly can't afford to do this for free. At a minimum, I think the patient should pay the mailing charge. Thank you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 17 Date: Thu, 18 Oct 2007 12:45:04 -0400 From: "Weems, Joyce" Subject: RE: [Histonet] Charging for patient slide send out To: "Richard Cartun" , Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F3F7D@sjhaexc02.sjha.org> Content-Type: text/plain; charset="utf-8" We have patient pay shipping charge and have facilities that will bill pt ins for second opinion. So many consultants are changing to bill client only that it has really become a problem. j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Richard Cartun Sent: Thursday, October 18, 2007 12:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Charging for patient slide send out I know we have discussed this before, but how many of you in clinical pathology labs are doing direct patient billing (cash or credit card) for sending out patient slides for second opinions? We are getting so many requests now we cannot keep up, and we certainly can't afford to do this for free. At a minimum, I think the patient should pay the mailing charge. Thank you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 47, Issue 22 **************************************** Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s), and may contain privileged or confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by replying to this email, and destroy all copies of the original message. From lisab <@t> hollandhospital.org Thu Oct 18 13:20:10 2007 From: lisab <@t> hollandhospital.org (Lisa Brenner) Date: Thu Oct 18 13:21:01 2007 Subject: [Histonet] Mats for the histology lab Message-ID: <47176B99.3CA1.003C.0@hollandhospital.org> Hello, We recently moved into our brand new lab space. We are trying to keep the floors nice which is no easy feat in a histology lab as you all know. My question is what do all you out there recommend for mats for floors by the cutting and embedding stations? We are hoping to find something that protects the floor, is easy to roll your chair on, and doesn't produce static. Any feed back is appreciated. Thanks and have a great day! Lisa Brenner HTL (ASCP) Histology Technical Consultant Holland Hospital phone: (616)394-3184 lisab@hollandhospital.org Confidentiality Notice: The information contained in this e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information, or Protected Health Information as such term is defined under the Health Insurance Portability and Accountability Act of 1996 (HIPAA). Any unauthorized review, use, disclosure, copying or distribution is prohibited and may be unlawful. If you believe you have received this e-mail in error, please contact the sender by reply e-mail and delete all copies of the original message, including attachments. From mlm11 <@t> cornell.edu Thu Oct 18 13:25:22 2007 From: mlm11 <@t> cornell.edu (Mary Lou Norman) Date: Thu Oct 18 13:24:27 2007 Subject: [Histonet] aluminum block molds Message-ID: <6.2.1.2.2.20071018142048.044a16b0@postoffice9.mail.cornell.edu> Hi, Anybody interested in the old, old style paraffin block molds? These are the ones that look similar to combs and they hook together by the teeth. Thanks, Mary Lou From cmiller <@t> physlab.com Thu Oct 18 13:25:19 2007 From: cmiller <@t> physlab.com (Cheri Miller) Date: Thu Oct 18 13:25:27 2007 Subject: [Histonet] FW: PAS/Fungus Message-ID: <000301c811b4$3ef099c0$3402a8c0@plab.local> Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _____ From: Cheri Miller [mailto:cmiller@physlab.com] Sent: Wednesday, October 17, 2007 10:38 AM To: histonet-bounces@lists.utsouthwestern.edu Subject: PAS/Fungus I have a Path that says our PAS/F is weak. His suggestion is that I do a hematoxilyn counter instead of light green. Anyone else doing this?? Seems to me that the counter stain won't make the PAS reaction any stronger. Our procedure is as follows; .5%Periodic acid 5 mins 2 changes DI water Schiff's 20 mins Running water 10 mins Light green 1 min 95,100,100 and 3 xylene I don't get any complaints from the other 6 pathologist's. Share your wisdom. Thanks Cheri Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From Nancy.Warren <@t> nkch.org Thu Oct 18 13:26:48 2007 From: Nancy.Warren <@t> nkch.org (Nancy Warren) Date: Thu Oct 18 13:27:11 2007 Subject: [Histonet] You can charge per slide. We charge $20 per slide for referrals outside of diagnostic purposes. Research, legal or for someones interest. It must be across the board with no exceptions. Message-ID: <77FADC01535C0B4FAC7EC79961E9B73C5E6A4F@Belgian.nkch.org> You can charge per slide. We charge $20 per slide for referrals outside of diagnostic purposes. Research, legal or for someones interest. It must be across the board with no exceptions. It has cut down on the number of requests and re-requests because of lost slides. Some doctors get mad, but stick to your guns and you will get this accomplished and not be going crazy with requests. Skippi ext. 5041 Be kind to unkind people (they probably need it the most). Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s), and may contain privileged or confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by replying to this email, and destroy all copies of the original message. From b-frederick <@t> northwestern.edu Thu Oct 18 13:28:12 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Oct 18 13:28:25 2007 Subject: [Histonet] Charging for patient slide send out In-Reply-To: <4717537C02000077000089BD@gwmail4.harthosp.org> Message-ID: <000501c811b4$a6f97190$d00f7ca5@lurie.northwestern.edu> Isn't there a CPT code for consultation? Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, October 18, 2007 11:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Charging for patient slide send out I know we have discussed this before, but how many of you in clinical pathology labs are doing direct patient billing (cash or credit card) for sending out patient slides for second opinions? We are getting so many requests now we cannot keep up, and we certainly can't afford to do this for free. At a minimum, I think the patient should pay the mailing charge. Thank you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Thu Oct 18 13:29:27 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Thu Oct 18 13:29:45 2007 Subject: [Histonet] Charging for patient slide send out In-Reply-To: <000501c811b4$a6f97190$d00f7ca5@lurie.northwestern.edu> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F3F88@sjhaexc02.sjha.org> It's professional only - none for the actual send out charges. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Bernice Frederick Sent: Thursday, October 18, 2007 2:28 PM To: 'Richard Cartun'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Charging for patient slide send out Isn't there a CPT code for consultation? Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, October 18, 2007 11:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Charging for patient slide send out I know we have discussed this before, but how many of you in clinical pathology labs are doing direct patient billing (cash or credit card) for sending out patient slides for second opinions? We are getting so many requests now we cannot keep up, and we certainly can't afford to do this for free. At a minimum, I think the patient should pay the mailing charge. Thank you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From b-frederick <@t> northwestern.edu Thu Oct 18 13:30:08 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Oct 18 13:30:25 2007 Subject: [Histonet] RE: world series/monday night football/NSH In-Reply-To: Message-ID: <000601c811b4$ec21c240$d00f7ca5@lurie.northwestern.edu> I won't be there- Dancing with the Stars is on and I am a NASCAR fan!!!!! Bad enough I have to tape the race!!!!! Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bell, Lynne Sent: Thursday, October 18, 2007 12:48 PM To: Molinari, Betsy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: world series/monday night football/NSH Betsy, I feel your pain - I'm a life long Red Sox fan as well....... Unfortunately, I won't be at the NSH convention. Lynne _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sheila_adey <@t> hotmail.com Thu Oct 18 13:35:19 2007 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Thu Oct 18 13:35:23 2007 Subject: [Histonet] Tris buffer Message-ID: Hello netters. Can anyone tell me the function of the pre soak in Tris buffer, prior to the IHC? Also, how long are you pre soaking your IHCs? Thanks in advance Sheila Adey HT MLTPort Huron HospitalMichigan _________________________________________________________________ Are you ready for Windows Live Messenger Beta 8.5 ? Get the latest for free today! http://entertainment.sympatico.msn.ca/WindowsLiveMessenger From failm <@t> musc.edu Thu Oct 18 13:39:09 2007 From: failm <@t> musc.edu (Mildred Fail) Date: Thu Oct 18 13:39:28 2007 Subject: [Histonet] FW: PAS/Fungus Message-ID: We use chromic acid as the oxidizer, only the fungi stains with Schiff's, tissue is counter stained in light green. Because of the large number of specimens stained with Scoff's, I have requested that it be changed each week assuring consistency week to week. We found that when the Schiff;s reaction appeared to be too light, simply using fresh fixed the problem Rena Fail >>> "Cheri Miller" 10/18/07 02:25PM >>> Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _____ From: Cheri Miller [mailto:cmiller@physlab.com] Sent: Wednesday, October 17, 2007 10:38 AM To: histonet-bounces@lists.utsouthwestern.edu Subject: PAS/Fungus I have a Path that says our PAS/F is weak. His suggestion is that I do a hematoxilyn counter instead of light green. Anyone else doing this?? Seems to me that the counter stain won't make the PAS reaction any stronger. Our procedure is as follows; ..5%Periodic acid 5 mins 2 changes DI water Schiff's 20 mins Running water 10 mins Light green 1 min 95,100,100 and 3 xylene I don't get any complaints from the other 6 pathologist's. Share your wisdom. Thanks Cheri Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lange <@t> kennedykrieger.org Thu Oct 18 13:41:34 2007 From: lange <@t> kennedykrieger.org (Mollie Lange) Date: Thu Oct 18 13:42:07 2007 Subject: [Histonet] thick paraffin sections for stereology Message-ID: <4717703B.BB3C.0024.0@kennedykrieger.org> Hello Everyone, Help! I need some fixation and processing alternatives. We need to cut 20-30 micron thick paraffin sections of post-natal day 7-14 mouse brains. Immunocytochemistry for apoptosis inducing factor (AIF) will be performed and then the positive cells will be counted using unbiased stereology (optical fractionator). Fixation is 4% paraformaldehyde perfusion with a 4-6hr post-fixation. We dehydrate in graded ethanols, clear in cedarwood oil and methyl salicylate and embed in paraffin. Processing steps are as short as possible to ensure adequate dehydration, clearing and infiltration. All steps are done by hand. This protocol is okay for 6-10 micron sections, but not for the required thicker sections. The tissue just completely shreds. Soaking the block face does not give good results. Methacarn fixation is good for cutting thicker sections, but unfortunately it is not compatible with ICC for AIF. Are there other fixation, processing or sectioning procedures we should try? Bouin's? Methanol? Immersion rather than perfusion? Any and all insights are most appreciated. Thanks, Mollie Lange International Center for Spinal Cord Injury Kennedy Krieger Institute Baltimore, MD 21205 Disclaimer: The materials in this e-mail are private and may contain Protected Health Information. Please note that e-mail is not necessarily confidential or secure. Your use of e-mail constitutes your acknowledgment of these confidentiality and security limitations. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution, or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via telephone or return e-mail. From BMolinari <@t> heart.thi.tmc.edu Thu Oct 18 13:46:30 2007 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Thu Oct 18 13:46:35 2007 Subject: [Histonet] NSH S/C in Denver In-Reply-To: <008f01c811b2$8c9bc9d0$6601a8c0@Patsy> Message-ID: Thank you Patsy for all the info! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Thursday, October 18, 2007 1:13 PM To: 'Histonet' Subject: [Histonet] NSH S/C in Denver NSH moved the S/C to Denver and a World Series broke out. World Series games on Oct.27, 28 and 29 are expected to be in Denver (yes it will be crazy downtown where the meeting is taking place, god help us find places to eat and park, etc.) If you want to try and get tickets to a game, they go on sale next Monday Oct. 22 at 10 am online only at www.coloradorockies.com . Hope to see you in Denver, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC 12635 Montview Blvd. Ste.215 Aurora, Colorado 80045 Phone: 720-859-4060 Fax: 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Thu Oct 18 13:51:47 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Oct 18 13:52:01 2007 Subject: [Histonet] IgG4 - IHC Message-ID: <471773030200007700008A17@gwmail4.harthosp.org> Is anyone doing IHC for IgG4? Thanks. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From Charles.Embrey <@t> carle.com Thu Oct 18 13:53:02 2007 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Thu Oct 18 13:54:05 2007 Subject: [Histonet] Pathologist's Assistant In-Reply-To: <9D12D4EF30176D4F839EB47F3D0E8436027B8605@exbk2.maxhealth.com> Message-ID: <44780C571F28624DBB446DE55C4D733A1FE55E@EXCHANGEBE1.carle.com> A Pathologists' assistant or pathology assistant? Also are you talking about certified or not in the case of the Pathologists' Assistant? Charles Embrey, PA(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eric Weber Sent: Thursday, October 18, 2007 1:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathologist's Assistant Looking for help, Can anyone explain the requirements for a Pathology Assistant? Thank you. Eric Weber Maxim Healthcare Services Toll Free (877) 263-7823 Toll Free Fax (866) 466-2981 erweber@maxhealth.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From david.kinsley <@t> spcorp.com Thu Oct 18 14:09:22 2007 From: david.kinsley <@t> spcorp.com (Kinsley, David) Date: Thu Oct 18 14:09:27 2007 Subject: [Histonet] changing processor reagents Message-ID: Hi, I would like to know what criteria people are using to determine when to change or rotate reagents on their tissue processors. Is there a certain # of cassettes processed, or # of processing runs, or other criteria? Do you take into consideration the type of tissue processed? We are using a Thermo-Shandon Excelsior, and find that the reagent quality monitor is not a reliable tool for assessing reagent quality and are looking to establish some new guidelines for reagent rotation. Any advice is appreciated. Thanks Dave. ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From Lynn.Burton <@t> Illinois.gov Thu Oct 18 14:16:35 2007 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Thu Oct 18 14:18:12 2007 Subject: [Histonet] changing processor reagents References: Message-ID: On our old processor, which we use for CWD, we change cleaning reagents at least every three runs. We change or rotate reagents every 5 runs or 300 blocks whichever comes first. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Kinsley, David Sent: Thu 10/18/2007 2:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] changing processor reagents Hi, I would like to know what criteria people are using to determine when to change or rotate reagents on their tissue processors. Is there a certain # of cassettes processed, or # of processing runs, or other criteria? Do you take into consideration the type of tissue processed? We are using a Thermo-Shandon Excelsior, and find that the reagent quality monitor is not a reliable tool for assessing reagent quality and are looking to establish some new guidelines for reagent rotation. Any advice is appreciated. Thanks Dave. ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bliven.laura <@t> marshfieldclinic.org Thu Oct 18 14:22:21 2007 From: bliven.laura <@t> marshfieldclinic.org (bliven.laura@marshfieldclinic.org) Date: Thu Oct 18 14:22:27 2007 Subject: [Histonet] Disposal of Fixed Tissues Message-ID: <558bf01c811bc$34dcab60$7205010a@mfldclinframe.org> Will anyone please share your info on how you dispose of your formalin-fixed wet tissue, OTHER than incineration? In the future, our incinerator will be closing down permanently. We will need to dispose of a fair amount of tissue every week. Any idea of cost will also be appreciated. Thanks, Laura Laura Bliven Histology Lab/IHC Marshfield Laboratories 1000 N. Oak Ave. Marshfield, WI 54449 From MSHERWOOD <@t> PARTNERS.ORG Thu Oct 18 14:28:55 2007 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Thu Oct 18 14:29:00 2007 Subject: [Histonet] Re: PPM1A Ab Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D97B11C56@PHSXMB30.partners.org> To all: I have a researcher who wants to run PPM1A Ab on mouse colon tissue. The Ab he bought (from abcam) has only been run on western blots and ELISA. 1. Has anybody any experience with this Ab? Have you used it on tissue (frozen or paraffin)? (PPM1A is a Mn2+ or Mg2+-dependent protein serine/threonine phosphatase that inhibits human stress-responsive p38 and JNK MAPK pathways and regulates cellular stress response in eukaryotes). 2. Or do you know of another company that sells this Ab for paraffin/frozen use? Hope someone can assist me. Thanks! Peggy I will not be attending the NSH meeting in Denver, but, being from Boston, I am a diehard Red Sox fan, so I will be glued to my TV set tonight, hoping for a turnaround! Go Sox! Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From MSHERWOOD <@t> PARTNERS.ORG Thu Oct 18 14:33:04 2007 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Thu Oct 18 14:33:09 2007 Subject: [Histonet] Disposal of Fixed Tissues In-Reply-To: <558bf01c811bc$34dcab60$7205010a@mfldclinframe.org> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D97B11C57@PHSXMB30.partners.org> Check with your local hospital to see how they dispose of their human waste. In MGH research labs, we have red bins for disposing of contaminated pipets, etc. as well as waste tissue. These are collected by Environmental Services when 3/4 full. I believe they are sent off-site for disposal. Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of bliven.laura@marshfieldclinic.org Sent: Thursday, October 18, 2007 3:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Disposal of Fixed Tissues Will anyone please share your info on how you dispose of your formalin-fixed wet tissue, OTHER than incineration? In the future, our incinerator will be closing down permanently. We will need to dispose of a fair amount of tissue every week. Any idea of cost will also be appreciated. Thanks, Laura Laura Bliven Histology Lab/IHC Marshfield Laboratories 1000 N. Oak Ave. Marshfield, WI 54449 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From lpjones <@t> srhs-pa.org Thu Oct 18 14:50:57 2007 From: lpjones <@t> srhs-pa.org (Jones, Laura) Date: Thu Oct 18 14:51:14 2007 Subject: [Histonet] New CAP Regulations - Breast Tissue Must Be Fixed in Formalin Message-ID: <8E7AD740937B954F947F0DB4467EFEE056E9F2@mail.srhs-pa.org> Hi all. I am wondering if anyone else out there is NOT using formalin on a routine basis any more, and now must bring it back in order to comply with the new CAP regulation. We routinely process everything in Shandon's Glyofixx. Our Pathologist would like to have breast tissues only submitted in formalin, left overnight to fix, and then process from alcohols on during the next day. He feels that the small biopsies will be our main concern, as these are what we usually send for ER/PR, Her2Neu and FISH. If anyone else is going through this same situation, we'd appreciate hearing how you are handling this. Also, a question from my managers: in this situation, can we charge anything for the "special" treatment of the tissue? My feeling was this it was kind of our "fault" that we had to do this, so there probably could not be a charge, but I told them I'd ask the experts. Thanks to all in advance. Laura From akemiat3377 <@t> yahoo.com Thu Oct 18 15:01:58 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Thu Oct 18 15:02:06 2007 Subject: [Histonet] NSH S/C in Denver In-Reply-To: <008f01c811b2$8c9bc9d0$6601a8c0@Patsy> Message-ID: <847621.80295.qm@web31312.mail.mud.yahoo.com> Hi All, This is going to be a crazy time. I Bet a lot of the the men will have a hard choice on getting scalped tickets to the game or attending NSH! Hope they don't give our rooms away for more $$$. Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL Client Service Manager PhenoPath Laboratories 551 N 34th St., #100 Seattle, WA 98103 (206) 374-9000 akemi@phenopath.com http://www.phenopath.com --- Patsy Ruegg wrote: > NSH moved the S/C to Denver and a World Series broke > out. World Series > games on Oct.27, 28 and 29 are expected to be in > Denver (yes it will be > crazy downtown where the meeting is taking place, > god help us find places to > eat and park, etc.) If you want to try and get > tickets to a game, they go > on sale next Monday Oct. 22 at 10 am online only at > www.coloradorockies.com > . > > > > Hope to see you in Denver, > > > > Patsy > > > > Patsy Ruegg, HT(ASCP)QIHC > > IHCtech, LLC > > 12635 Montview Blvd. Ste.215 > > Aurora, Colorado 80045 > > Phone: 720-859-4060 > > Fax: 720-859-4110 > > pruegg@ihctech.net > > www.ihctech.net > > www.ihcrg.org > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Akemi Allison-Tacha, BS, HT(ASCP)HTL President Phoenix Lab Consulting & Staffing Tele: (925)788-0900 E-Mail: akemiat3377@yahoo.com From detmar <@t> mshri.on.ca Thu Oct 18 15:09:24 2007 From: detmar <@t> mshri.on.ca (Jacqui Detmar) Date: Thu Oct 18 15:09:42 2007 Subject: [Histonet] mouse spleen and Prussian Blue stain Message-ID: Hello all. I have a couple of blocks embedded with mouse spleen tissue, one which contains older mouse spleen (6 months old) and one with younger mouse spleen (3 weeks). These tissues are further sub-grouped as coming from mice wildtype for caspase-2 or deleted for caspase-2. I wanted to assess the spleen morphology between the two strains b/c the spleen from the deleted mouse appears to be larger as the mouse is older (i.e. spleen from deleted versus wildtype at 3 weeks is same size). The morphology looked a little wonky, so I did a Prussian Blue stain. I have never done this stain before in mouse spleen and before I go embedding some more tissue, I would like to get some info from people who might already know the answer. The deleted and wildtype spleens from the 3-week-old mice looked similar with respect to Prussian blue stain: very few blue deposits in the tissue. In the older tissue, I saw a great deal of iron deposition in the wildtype spleen but almost no deposition in the deleted spleen. This is opposite to what I hypothesized. I thought the deleted spleen would be more damaged and have a greater amount of blue staining. Now I'm worried that the tissue might have been switched during collection or processing - although the size of the tissue in the block and on the section corresponds to the increased spleen size in the deleted mouse. Does anybody out there know what an elderly mouse spleen should look like after Prussian blue staining? Thanks, Jacqui Detmar, Post-doctoral Fellow Samuel Lunenfeld Research Institute, room 876 Mount Sinai Hospital 600 University Avenue Toronto, ON, Canada M5G 1X5 Tel: 416-586-4800 x2451 Fax: 416-586-8588 Email: detmar@mshri.on.ca From nfournier <@t> sasktel.net Thu Oct 18 15:11:49 2007 From: nfournier <@t> sasktel.net (N Fournier) Date: Thu Oct 18 15:11:57 2007 Subject: [Histonet] removing a coverslip Message-ID: Hi everyone, I need to remove a coverslip from tissue that was stained previously for GAD67, so that I can counterstain the tissue with cresyl violet but also retain the previous immunostain. What is the best method to remove a coverslip ? The slides are Superfrost Plus, the tissue is rat brain, and mounting medium is DPX or Entellan (depending on experiment). One recommendation I received was to leave the slides in xylene for several days and try to pry of the tissue that way. I would think that prolonged xylene exposure might influence the staining of my tissue and secondly it seems to be taking forever. If the xylene method is the best then I do not mind waiting, but I really need to get this tissue counterstained ASAP. So any recomendations are welcomed. And lastly, once the coverslip has been removed do I need to run the slides through descending alcohols and water before I start my cresyl violet staining protocol? If this is required, does anyone have handy the concentrations of alcohol and how long the slides are to be kept in these solutions? I really appreciate the help, Neil From rjbuesa <@t> yahoo.com Thu Oct 18 16:09:23 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 18 16:09:26 2007 Subject: [Histonet] Pathologist's Assistant In-Reply-To: <9D12D4EF30176D4F839EB47F3D0E8436027B8605@exbk2.maxhealth.com> Message-ID: <325319.13525.qm@web61219.mail.yahoo.com> Check their website Ren? J. Eric Weber wrote: Looking for help, Can anyone explain the requirements for a Pathology Assistant? Thank you. Eric Weber Maxim Healthcare Services Toll Free (877) 263-7823 Toll Free Fax (866) 466-2981 erweber@maxhealth.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From rjbuesa <@t> yahoo.com Thu Oct 18 16:11:14 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 18 16:11:20 2007 Subject: [Histonet] Tris buffer In-Reply-To: Message-ID: <924865.29103.qm@web61211.mail.yahoo.com> The idea is that the slides you have hydrated (after dewaxing) should be immersed (soaked if you like) in a liquid with a neutral pH (either Tris or PBS). Ren? J. sheila adey wrote: Hello netters. Can anyone tell me the function of the pre soak in Tris buffer, prior to the IHC? Also, how long are you pre soaking your IHCs? Thanks in advance Sheila Adey HT MLTPort Huron HospitalMichigan _________________________________________________________________ Are you ready for Windows Live Messenger Beta 8.5 ? Get the latest for free today! http://entertainment.sympatico.msn.ca/WindowsLiveMessenger_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From rjbuesa <@t> yahoo.com Thu Oct 18 16:13:19 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 18 16:13:25 2007 Subject: [Histonet] changing processor reagents In-Reply-To: Message-ID: <168698.66018.qm@web61213.mail.yahoo.com> Using "runs" as a criteria is not very objective. Use the number of cassettes. I always rotated some reagents/discarded the oldest, every time the TP capacity was reached. Ren? J. "Kinsley, David" wrote: Hi, I would like to know what criteria people are using to determine when to change or rotate reagents on their tissue processors. Is there a certain # of cassettes processed, or # of processing runs, or other criteria? Do you take into consideration the type of tissue processed? We are using a Thermo-Shandon Excelsior, and find that the reagent quality monitor is not a reliable tool for assessing reagent quality and are looking to establish some new guidelines for reagent rotation. Any advice is appreciated. Thanks Dave. ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From rjbuesa <@t> yahoo.com Thu Oct 18 16:14:40 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 18 16:14:43 2007 Subject: [Histonet] Disposal of Fixed Tissues In-Reply-To: <558bf01c811bc$34dcab60$7205010a@mfldclinframe.org> Message-ID: <401339.66018.qm@web61213.mail.yahoo.com> Other than incineration, you will have to use a specialized contractor, probably the same you use to dispose the formalin. Renj J. bliven.laura@marshfieldclinic.org wrote: Will anyone please share your info on how you dispose of your formalin-fixed wet tissue, OTHER than incineration? In the future, our incinerator will be closing down permanently. We will need to dispose of a fair amount of tissue every week. Any idea of cost will also be appreciated. Thanks, Laura Laura Bliven Histology Lab/IHC Marshfield Laboratories 1000 N. Oak Ave. Marshfield, WI 54449 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From rjbuesa <@t> yahoo.com Thu Oct 18 16:18:38 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 18 16:18:45 2007 Subject: [Histonet] New CAP Regulations - Breast Tissue Must Be Fixed in Formalin In-Reply-To: <8E7AD740937B954F947F0DB4467EFEE056E9F2@mail.srhs-pa.org> Message-ID: <650091.94464.qm@web61212.mail.yahoo.com> This CAP (and FDA) regulation is a hindrance in any efforts to eliminate carcinogenic formalin from the histo lab, but has to be followed to be in compliance, or to participate in studies about breast cancer. This fact does not qualify for a special treatment/charge. Ren? J. "Jones, Laura" wrote: Hi all. I am wondering if anyone else out there is NOT using formalin on a routine basis any more, and now must bring it back in order to comply with the new CAP regulation. We routinely process everything in Shandon's Glyofixx. Our Pathologist would like to have breast tissues only submitted in formalin, left overnight to fix, and then process from alcohols on during the next day. He feels that the small biopsies will be our main concern, as these are what we usually send for ER/PR, Her2Neu and FISH. If anyone else is going through this same situation, we'd appreciate hearing how you are handling this. Also, a question from my managers: in this situation, can we charge anything for the "special" treatment of the tissue? My feeling was this it was kind of our "fault" that we had to do this, so there probably could not be a charge, but I told them I'd ask the experts. Thanks to all in advance. Laura _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From rjbuesa <@t> yahoo.com Thu Oct 18 16:19:34 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 18 16:19:38 2007 Subject: [Histonet] removing a coverslip In-Reply-To: Message-ID: <422106.97385.qm@web61221.mail.yahoo.com> The recommendation you received is the best. Ren? J. N Fournier wrote: Hi everyone, I need to remove a coverslip from tissue that was stained previously for GAD67, so that I can counterstain the tissue with cresyl violet but also retain the previous immunostain. What is the best method to remove a coverslip ? The slides are Superfrost Plus, the tissue is rat brain, and mounting medium is DPX or Entellan (depending on experiment). One recommendation I received was to leave the slides in xylene for several days and try to pry of the tissue that way. I would think that prolonged xylene exposure might influence the staining of my tissue and secondly it seems to be taking forever. If the xylene method is the best then I do not mind waiting, but I really need to get this tissue counterstained ASAP. So any recomendations are welcomed. And lastly, once the coverslip has been removed do I need to run the slides through descending alcohols and water before I start my cresyl violet staining protocol? If this is required, does anyone have handy the concentrations of alcohol and how long the slides are to be kept in these solutions? I really appreciate the help, Neil _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From Lance.Erickson <@t> intermountainmail.org Thu Oct 18 16:27:22 2007 From: Lance.Erickson <@t> intermountainmail.org (Lance Erickson) Date: Thu Oct 18 16:27:28 2007 Subject: [Histonet] Pathologist's Assistant In-Reply-To: <325319.13525.qm@web61219.mail.yahoo.com> References: <9D12D4EF30176D4F839EB47F3D0E8436027B8605@exbk2.maxhealth.com> <325319.13525.qm@web61219.mail.yahoo.com> Message-ID: <8B08EC394B366D4A895DD5686D6AFE4AE7E92A@LP-EXCHVS08.CO.IHC.COM> www.pathologistsassistants.org "about us" section "What is a PA". www.ascp.org/bor to find out about the certification/qualifications/requirements. -Lance, Primary Children's Medical Center, SLC, UT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, October 18, 2007 3:09 PM To: Eric Weber; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Pathologist's Assistant Check their website Ren? J. Eric Weber wrote: Looking for help, Can anyone explain the requirements for a Pathology Assistant? Thank you. Eric Weber Maxim Healthcare Services Toll Free (877) 263-7823 Toll Free Fax (866) 466-2981 erweber@maxhealth.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From m5johnso <@t> meded.ucsd.edu Thu Oct 18 16:30:24 2007 From: m5johnso <@t> meded.ucsd.edu (Mindy Johnson) Date: Thu Oct 18 16:30:48 2007 Subject: [Histonet] removing a coverslip In-Reply-To: <422106.97385.qm@web61221.mail.yahoo.com> References: <422106.97385.qm@web61221.mail.yahoo.com> Message-ID: <00fc01c811ce$18740150$495c03f0$@ucsd.edu> Yes it takes a long time, but I too have found this is what works best. In fact I have had to restain several hundred slides here and that is what I have done. If it's a few slides, I would change the xylene a few times cause I ruined a few when I didn't. The mountant wasn't all removed. However, I haven't had a problem with the prolonged xylene exposure and restaining. Good luck! :) ?Mindy A Johnson SRA II UCSD - School of Medicine Medical Teaching Labs -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, October 18, 2007 2:20 PM To: N Fournier; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] removing a coverslip The recommendation you received is the best. Ren? J. N Fournier wrote: Hi everyone, I need to remove a coverslip from tissue that was stained previously for GAD67, so that I can counterstain the tissue with cresyl violet but also retain the previous immunostain. What is the best method to remove a coverslip ? The slides are Superfrost Plus, the tissue is rat brain, and mounting medium is DPX or Entellan (depending on experiment). One recommendation I received was to leave the slides in xylene for several days and try to pry of the tissue that way. I would think that prolonged xylene exposure might influence the staining of my tissue and secondly it seems to be taking forever. If the xylene method is the best then I do not mind waiting, but I really need to get this tissue counterstained ASAP. So any recomendations are welcomed. And lastly, once the coverslip has been removed do I need to run the slides through descending alcohols and water before I start my cresyl violet staining protocol? If this is required, does anyone have handy the concentrations of alcohol and how long the slides are to be kept in these solutions? I really appreciate the help, Neil _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lance.Erickson <@t> intermountainmail.org Thu Oct 18 16:39:20 2007 From: Lance.Erickson <@t> intermountainmail.org (Lance Erickson) Date: Thu Oct 18 16:39:38 2007 Subject: [Histonet] New CAP Regulations - Breast Tissue Must Be Fixed inFormalin In-Reply-To: <650091.94464.qm@web61212.mail.yahoo.com> References: <8E7AD740937B954F947F0DB4467EFEE056E9F2@mail.srhs-pa.org> <650091.94464.qm@web61212.mail.yahoo.com> Message-ID: <8B08EC394B366D4A895DD5686D6AFE4AE7E938@LP-EXCHVS08.CO.IHC.COM> There is a new CAP checklist for Anatomic Pathology that was updated on the CAP website October 5th. These changes are effective September 27, 2007. All of the new checklist questions involve Her2 testing both IHC and FISH. And actually there is a question that addresses the use of fixatives other than formalin. Look at the notes section of question ANP 22997 you must validate your method with a minimum of 25 (recommended 25-100)and if you use a fixative other than formalin you must show that the results are concordant with the results from formalin-fixed tissues. The next new question specifically addresses the length of time in fixative. Take a look at these new questions at www.cap.org go to the "accreditation and laboratory improvement" tab under the inspection information click the "inspection checklists" and print off the new AP questions. -Lance, Primary Children's, SLC,UT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, October 18, 2007 3:19 PM To: Jones, Laura; Histonet (E-mail) Subject: Re: [Histonet] New CAP Regulations - Breast Tissue Must Be Fixed inFormalin This CAP (and FDA) regulation is a hindrance in any efforts to eliminate carcinogenic formalin from the histo lab, but has to be followed to be in compliance, or to participate in studies about breast cancer. This fact does not qualify for a special treatment/charge. Ren? J. "Jones, Laura" wrote: Hi all. I am wondering if anyone else out there is NOT using formalin on a routine basis any more, and now must bring it back in order to comply with the new CAP regulation. We routinely process everything in Shandon's Glyofixx. Our Pathologist would like to have breast tissues only submitted in formalin, left overnight to fix, and then process from alcohols on during the next day. He feels that the small biopsies will be our main concern, as these are what we usually send for ER/PR, Her2Neu and FISH. If anyone else is going through this same situation, we'd appreciate hearing how you are handling this. Also, a question from my managers: in this situation, can we charge anything for the "special" treatment of the tissue? My feeling was this it was kind of our "fault" that we had to do this, so there probably could not be a charge, but I told them I'd ask the experts. Thanks to all in advance. Laura _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From paul.kammeyer <@t> comphealth.com Thu Oct 18 17:14:02 2007 From: paul.kammeyer <@t> comphealth.com (paul.kammeyer@comphealth.com) Date: Thu Oct 18 17:09:52 2007 Subject: [Histonet] Pathologists' Assistant Opportunity Message-ID: Hello! With the recent talk and inquiries about the qualifications of a Pathologists' Assistant, I thought I would submit my own inquiry. Currently, I work with a facility in Texas that would like someone to fill a permanent position they have available. The position would be a 1st shift and the other details would be worked out. If you are interested or know someone else who would be interested call me to discuss. I appreciate your time and Happy Thursday! Paul Kammeyer Staffing Consultant Lab Specialties - CompHealth P.O. Box 713400 Salt Lake City, UT 84171-3400 Phone: (800) 447-6016 ext. 3380 Fax: (866) 588-1340, (801) 930-4504 paul.kammeyer@comphealth.com www.comphealth.com Ask me about our $500 Referral Bonus Program!! From jnocito <@t> satx.rr.com Thu Oct 18 17:40:26 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Oct 18 17:40:30 2007 Subject: [Histonet] Ventana Benchmark References: <471714D6.26ED.00D9.0@nehealth.com> Message-ID: <002301c811d7$e1673f60$0302a8c0@yourxhtr8hvc4p> my last place of employment has 3 XTs. It is my experience that it is hit or miss with these machines. The first machine has been breaking down ever since it was purchased. The other two have had a hiccup or two, but have been ok. I have complained (here and to Ventana) about the situation. When do you consider the machine a total loss and replace it? I have a 1991 Ford pick up that I keep thowing money into to it, but it doesn't handle patient specimens. I raised a stink so bad that some regional manager came down and promised me the world. As far as I know, that machine has been repaired several times after I left. I do have to give Ventana credit on one thing- they do try to get a service rep out as fast as they can. The problem is either they don't have enough service reps, their machines constantly break down, or both. Not to mention their prices are pretty steep. One has to look a bit past the purchase and research the entire company and product. Are they available for tech questions? Do you talk to a real person or have to leave a message and they'll get back with later, or tomorrow? Is your machine always breaking down for one thing or another? What happens if your machine is down for a while? Can you get a loaner? Are you getting lip service or is the company really trying to work with you? Like others have said, there are pros and cons for every machine and you can make some up your own. Try to test as many machines as you can, but also test the company and make sure that they can deliver what they say they can. One other thing, carefully review all contracts before signing, twice. If you have the availability to take the contract to a medical-legal office or someone else, please do. Joe ----- Original Message ----- From: "Amy Farnan" To: Sent: Thursday, October 18, 2007 7:09 AM Subject: [Histonet] Ventana Benchmark Hi everyone. I am looking for some feedback on the Ventana Benchmark stainer. I have a tech that worked with a Ventana stainer at her previous job and did not care for it. System was to closed for her. I have another tech that has not used the stainer but went to the recent Region II meeting and attended a workshop on evaluating IHC stainers. The Ventana Benchmark was not given favorable reviews. I do know some area hospitals that have it that really like it. Can you give me some pros and cons? Disclaimer: The information in this message is confidential. If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LINDA.MARGRAF <@t> childrens.com Thu Oct 18 17:42:59 2007 From: LINDA.MARGRAF <@t> childrens.com (LINDA MARGRAF) Date: Thu Oct 18 17:43:17 2007 Subject: [Histonet] replying to Histonet digest messages Message-ID: <47179B23020000DA0001651F@CNET3.CHILDRENS.COM> Dear Histonetters: I have had another request to remind people to please trim the unnecessary stuff from their messages before replying. It makes for huge documents if you include the digest in your response. This makes them hard to read and the messages are sometimes blocked by the servers. Please note.....On a Windows machine, you can use Ctrl-A - Del if you don't want to quote anything. If you want to quote something, you can first select and Ctrl-C the part you want to reply to, then use Ctrl-A and Del, and Ctrl-V to paste back the relevant bit.Thanks. I hope everyone going to the NSH meeting in Denver has a safe and fun trip. Regards, Linda M Histonet administrator From jnocito <@t> satx.rr.com Thu Oct 18 17:44:45 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Oct 18 17:44:49 2007 Subject: [Histonet] New CAP Regulations - Breast Tissue Must Be Fixed inFormalin References: <650091.94464.qm@web61212.mail.yahoo.com> Message-ID: <004301c811d8$7c698f40$0302a8c0@yourxhtr8hvc4p> This is why I have a problem with CAP. CAP can't regulate themselves and yet, they are regulating how to handle special specimens for special procedures. Give me a break. That said, I expect that I'll be getting flamed next week in Denver. Until then, flame on Joe ----- Original Message ----- From: "Rene J Buesa" To: "Jones, Laura" ; "Histonet (E-mail)" Sent: Thursday, October 18, 2007 4:18 PM Subject: Re: [Histonet] New CAP Regulations - Breast Tissue Must Be Fixed inFormalin > This CAP (and FDA) regulation is a hindrance in any efforts to eliminate > carcinogenic formalin from the histo lab, but has to be followed to be in > compliance, or to participate in studies about breast cancer. > This fact does not qualify for a special treatment/charge. > Ren? J. > > "Jones, Laura" wrote: > Hi all. I am wondering if anyone else out there is NOT using formalin on > a > routine basis any more, and now must bring it back in order to comply with > the new CAP regulation. We routinely process everything in Shandon's > Glyofixx. Our Pathologist would like to have breast tissues only submitted > in formalin, left overnight to fix, and then process from alcohols on > during > the next day. He feels that the small biopsies will be our main concern, > as > these are what we usually send for ER/PR, Her2Neu and FISH. If anyone else > is going through this same situation, we'd appreciate hearing how you are > handling this. > > Also, a question from my managers: in this situation, can we charge > anything for the "special" treatment of the tissue? My feeling was this it > was kind of our "fault" that we had to do this, so there probably could > not > be a charge, but I told them I'd ask the experts. Thanks to all in > advance. > Laura > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leba <@t> hawaii.edu Thu Oct 18 21:16:16 2007 From: leba <@t> hawaii.edu (Heather A Leba) Date: Thu Oct 18 21:16:58 2007 Subject: [Histonet] please unsubscribe me!!!! Message-ID: From BMolinari <@t> heart.thi.tmc.edu Fri Oct 19 05:41:53 2007 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri Oct 19 05:42:01 2007 Subject: [Histonet] NSH S/C in Denver In-Reply-To: <847621.80295.qm@web31312.mail.mud.yahoo.com> Message-ID: Why only the men? Betsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Thursday, October 18, 2007 3:02 PM To: Patsy Ruegg; 'Histonet' Subject: Re: [Histonet] NSH S/C in Denver Hi All, This is going to be a crazy time. I Bet a lot of the the men will have a hard choice on getting scalped tickets to the game or attending NSH! Hope they don't give our rooms away for more $$$. Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL Client Service Manager PhenoPath Laboratories 551 N 34th St., #100 Seattle, WA 98103 (206) 374-9000 akemi@phenopath.com http://www.phenopath.com --- Patsy Ruegg wrote: > NSH moved the S/C to Denver and a World Series broke > out. World Series > games on Oct.27, 28 and 29 are expected to be in > Denver (yes it will be > crazy downtown where the meeting is taking place, > god help us find places to > eat and park, etc.) If you want to try and get > tickets to a game, they go > on sale next Monday Oct. 22 at 10 am online only at > www.coloradorockies.com > . > > > > Hope to see you in Denver, > > > > Patsy > > > > Patsy Ruegg, HT(ASCP)QIHC > > IHCtech, LLC > > 12635 Montview Blvd. Ste.215 > > Aurora, Colorado 80045 > > Phone: 720-859-4060 > > Fax: 720-859-4110 > > pruegg@ihctech.net > > www.ihctech.net > > www.ihcrg.org > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Akemi Allison-Tacha, BS, HT(ASCP)HTL President Phoenix Lab Consulting & Staffing Tele: (925)788-0900 E-Mail: akemiat3377@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> CDC.GOV Fri Oct 19 05:50:54 2007 From: jqb7 <@t> CDC.GOV (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Fri Oct 19 05:51:10 2007 Subject: [Histonet] NSH S/C in Denver In-Reply-To: References: <847621.80295.qm@web31312.mail.mud.yahoo.com> Message-ID: <34BB307EFC9A65429BBB49E330675F72045E20A0@LTA3VS003.ees.hhs.gov> Good point Betsy. I love me some baseball! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Friday, October 19, 2007 6:42 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NSH S/C in Denver Why only the men? Betsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Thursday, October 18, 2007 3:02 PM To: Patsy Ruegg; 'Histonet' Subject: Re: [Histonet] NSH S/C in Denver Hi All, This is going to be a crazy time. I Bet a lot of the the men will have a hard choice on getting scalped tickets to the game or attending NSH! Hope they don't give our rooms away for more $$$. Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL Client Service Manager PhenoPath Laboratories 551 N 34th St., #100 Seattle, WA 98103 (206) 374-9000 akemi@phenopath.com http://www.phenopath.com --- Patsy Ruegg wrote: > NSH moved the S/C to Denver and a World Series broke out. World > Series games on Oct.27, 28 and 29 are expected to be in Denver (yes it > will be crazy downtown where the meeting is taking place, god help us > find places to eat and park, etc.) If you want to try and get tickets > to a game, they go on sale next Monday Oct. 22 at 10 am online only at > www.coloradorockies.com . > > > > Hope to see you in Denver, > > > > Patsy > > > > Patsy Ruegg, HT(ASCP)QIHC > > IHCtech, LLC > > 12635 Montview Blvd. Ste.215 > > Aurora, Colorado 80045 > > Phone: 720-859-4060 > > Fax: 720-859-4110 > > pruegg@ihctech.net > > www.ihctech.net > > www.ihcrg.org > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Akemi Allison-Tacha, BS, HT(ASCP)HTL President Phoenix Lab Consulting & Staffing Tele: (925)788-0900 E-Mail: akemiat3377@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From katia.catunda <@t> cipax.com.br Fri Oct 19 07:27:56 2007 From: katia.catunda <@t> cipax.com.br (=?iso-8859-1?Q?Ms._K=E1tia_Cristina_Catunda?=) Date: Fri Oct 19 06:27:39 2007 Subject: RES: [Histonet] changing processor reagents In-Reply-To: <168698.66018.qm@web61213.mail.yahoo.com> Message-ID: David, Here we have a routine of approximately 450 blocks each day and three tissue processors. We do change the last alcohol and xylene of the carroussel every single day (since the processor has been used). Once a week we change one of the two waxes. Hope it helps Ms. K?tia Catunda Produ??o +55 12 3203-0612 (direto) +55 12 3203-0633 (PABX) www.cipax.com.br katia.catunda@cipax.com.br From BMolinari <@t> heart.thi.tmc.edu Fri Oct 19 06:53:27 2007 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri Oct 19 06:53:31 2007 Subject: FW: [Histonet] NSH S/C in Denver/OT Message-ID: Not that I would shurck (sp) my responsibilities and obligations to NSH or my employers but I would be looking for tickets too,especially if the Sox are playing. My 50th b'day was in Sept and my dear husband took me to the Red Sox/Yankees SERIES at Fenway! It was incredible! -----Original Message----- From: Bartlett, Jeanine (CDC/CCID/NCZVED) [mailto:jqb7@CDC.GOV] Sent: Friday, October 19, 2007 5:51 AM To: Molinari, Betsy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NSH S/C in Denver Good point Betsy. I love me some baseball! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Friday, October 19, 2007 6:42 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NSH S/C in Denver Why only the men? Betsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Thursday, October 18, 2007 3:02 PM To: Patsy Ruegg; 'Histonet' Subject: Re: [Histonet] NSH S/C in Denver Hi All, This is going to be a crazy time. I Bet a lot of the the men will have a hard choice on getting scalped tickets to the game or attending NSH! Hope they don't give our rooms away for more $$$. Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL Client Service Manager PhenoPath Laboratories 551 N 34th St., #100 Seattle, WA 98103 (206) 374-9000 akemi@phenopath.com http://www.phenopath.com --- Patsy Ruegg wrote: > NSH moved the S/C to Denver and a World Series broke out. World > Series games on Oct.27, 28 and 29 are expected to be in Denver (yes it > will be crazy downtown where the meeting is taking place, god help us > find places to eat and park, etc.) If you want to try and get tickets > to a game, they go on sale next Monday Oct. 22 at 10 am online only at > www.coloradorockies.com . > > > > Hope to see you in Denver, > > > > Patsy > > > > Patsy Ruegg, HT(ASCP)QIHC > > IHCtech, LLC > > 12635 Montview Blvd. Ste.215 > > Aurora, Colorado 80045 > > Phone: 720-859-4060 > > Fax: 720-859-4110 > > pruegg@ihctech.net > > www.ihctech.net > > www.ihcrg.org > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Akemi Allison-Tacha, BS, HT(ASCP)HTL President Phoenix Lab Consulting & Staffing Tele: (925)788-0900 E-Mail: akemiat3377@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Karen.Heckford <@t> CHW.edu Fri Oct 19 06:59:15 2007 From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF) Date: Fri Oct 19 06:59:24 2007 Subject: [Histonet] Paraffin and Slide disposal. Message-ID: <2842DC75AE43AA4B92954CFB31781BC1821577@CHW-MSG-301.chw.edu> I have been handed a pretty big mess. We have a lot of old blocks and slides at a archiving company which needs to be disposed of because some date back to 1960. Does anyone know of a company that will come in and dispose of blocks and slides? Any input would be greatly appreciated. Cheers, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathology Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu From katia.catunda <@t> cipax.com.br Fri Oct 19 08:04:17 2007 From: katia.catunda <@t> cipax.com.br (=?iso-8859-1?Q?Ms._K=E1tia_Cristina_Catunda?=) Date: Fri Oct 19 07:03:56 2007 Subject: [Histonet] Histotechs In-Reply-To: Message-ID: Hi there!! Would like to know how many technicians each of you have working on: - stain - special stain - embedding - microtomy We have almost 500 blocks each day, 400 on autotech routine and 100 on microwave routine. We do have 10 technicians that alternates on microtomy (4), embedding (3), stain (1), slides ordenation (1) special stain (same on embedding) and block?s archivation (same on microtomy) and one technician on imunohistochemmistry (that occurs twice a week). We work every day from 7h00 AM to 4h30 PM for 5 pathologists. We have seen labs that has more blocks than us and less technicians.. I do really want to understand how they do that! Tks a lot!! Ms. K?tia Catunda Produ??o +55 12 3203-0612 (direto) +55 12 3203-0633 (PABX) www.cipax.com.br katia.catunda@cipax.com.br -----Mensagem original----- De: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Em nome de Ms. K?tia Cristina Catunda Enviada em: sexta-feira, 19 de outubro de 2007 09:28 Para: 'Kinsley, David'; histonet@lists.utsouthwestern.edu Assunto: RES: [Histonet] changing processor reagents David, Here we have a routine of approximately 450 blocks each day and three tissue processors. We do change the last alcohol and xylene of the carroussel every single day (since the processor has been used). Once a week we change one of the two waxes. Hope it helps Ms. K?tia Catunda Produ??o +55 12 3203-0612 (direto) +55 12 3203-0633 (PABX) www.cipax.com.br katia.catunda@cipax.com.br _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lynne.Bell <@t> hitchcock.org Fri Oct 19 07:13:17 2007 From: Lynne.Bell <@t> hitchcock.org (Bell, Lynne) Date: Fri Oct 19 07:13:21 2007 Subject: [Histonet] NSH S/C in Denver/OT In-Reply-To: Message-ID: Betsy, If Soxtober keeps going as we did last night, a Rockies-Red Sox World Series can be a possibility!! Go Sox, Lynne From lblazek <@t> digestivespecialists.com Fri Oct 19 07:22:56 2007 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Oct 19 07:20:19 2007 Subject: [Histonet] NSH S/C in Denver/OT In-Reply-To: References: Message-ID: <1F937FB30BDB7C4A9F39F83FEA8D379F3E4CA9@bruexchange1.digestivespecialists.com> Rockies - Indians -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bell, Lynne Sent: Friday, October 19, 2007 8:13 AM To: Molinari, Betsy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NSH S/C in Denver/OT Betsy, If Soxtober keeps going as we did last night, a Rockies-Red Sox World Series can be a possibility!! Go Sox, Lynne _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sheila_adey <@t> hotmail.com Fri Oct 19 07:30:20 2007 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Fri Oct 19 07:30:26 2007 Subject: [Histonet] Histotechs In-Reply-To: References: Message-ID: Hi, We do 110 - 150 blocks per day. We have 3 techs and 3 pathologists. We do have an automated special stainer which has been a huge time saver (Artisan). We do the pick ups from the ORs, FNAs, Cyto set up, grossing of small stuff, embedding, microtomy, IHCs and everything else that needs to be done like procedure writing, ordering of supplies, scheduling and autopsies. Sheila Adey HT MLTPort Huron HospitalMichigan> From: katia.catunda@cipax.com.br> To: histonet@lists.utsouthwestern.edu> Date: Fri, 19 Oct 2007 10:04:17 -0300> Subject: [Histonet] Histotechs> > Hi there!!> > Would like to know how many technicians each of you have working on:> > - stain> - special stain> - embedding> - microtomy> > We have almost 500 blocks each day, 400 on autotech routine and 100> on microwave routine. We do have 10 technicians that alternates on microtomy> (4), embedding (3), stain (1), slides ordenation (1) special stain (same on> embedding) and block?s archivation (same on microtomy) and one technician on> imunohistochemmistry (that occurs twice a week).> > We work every day from 7h00 AM to 4h30 PM for 5 pathologists.> > We have seen labs that has more blocks than us and less> technicians.. I do really want to understand how they do that!> > Tks a lot!!> > > > Ms. K?tia Catunda> Produ??o> +55 12 3203-0612 (direto)> +55 12 3203-0633 (PABX)> www.cipax.com.br> katia.catunda@cipax.com.br> > -----Mensagem original-----> De: histonet-bounces@lists.utsouthwestern.edu> [mailto:histonet-bounces@lists.utsouthwestern.edu] Em nome de Ms. K?tia> Cristina Catunda> Enviada em: sexta-feira, 19 de outubro de 2007 09:28> Para: 'Kinsley, David'; histonet@lists.utsouthwestern.edu> Assunto: RES: [Histonet] changing processor reagents> > David,> > Here we have a routine of approximately 450 blocks each day and> three tissue processors. > We do change the last alcohol and xylene of the carroussel every> single day (since the processor has been used). Once a week we change one of> the two waxes.> > Hope it helps> > > Ms. K?tia Catunda> Produ??o> +55 12 3203-0612 (direto)> +55 12 3203-0633 (PABX)> www.cipax.com.br> katia.catunda@cipax.com.br> > > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Are you ready for Windows Live Messenger Beta 8.5 ? Get the latest for free today! http://entertainment.sympatico.msn.ca/WindowsLiveMessenger From Teri.Hallada <@t> midmichigan.org Fri Oct 19 07:57:11 2007 From: Teri.Hallada <@t> midmichigan.org (Teri.Hallada@midmichigan.org) Date: Fri Oct 19 07:57:23 2007 Subject: [Histonet] Ventanna using other antibodies Message-ID: <8839B08E3ED7364E8CBBD53882C984D50994CB7D@MAILSRV01.midmichigan.net> I was wodering if there are others using antibodies not purchased from Ventanna on the Benchmark and what kind of validation studoes did you do? Teresa Hallada BS, MT/CT (ASCP) Lead Cytotechnologist MidMichigan Health - Gratiot teri.hallada@midmichigan.org 989.463.1101 ext 3423 Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law. The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are herby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited. If you have received this email in error, please advise by immediate reply. From Anthony.Johnson <@t> leica-microsystems.com Fri Oct 19 08:16:17 2007 From: Anthony.Johnson <@t> leica-microsystems.com (Anthony.Johnson@leica-microsystems.com) Date: Fri Oct 19 08:16:32 2007 Subject: [Histonet] Johnson, Anthony is out of the office. Message-ID: I will be out of the office starting 10/18/2007 and will not return until 10/24/2007. If your message is urgent please contact Luanda Diggs in our Customer Care dept on 847 405 7052. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From talulahgosh <@t> gmail.com Fri Oct 19 08:21:33 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Oct 19 08:21:38 2007 Subject: [Histonet] removing a coverslip In-Reply-To: <00fc01c811ce$18740150$495c03f0$@ucsd.edu> References: <422106.97385.qm@web61221.mail.yahoo.com> <00fc01c811ce$18740150$495c03f0$@ucsd.edu> Message-ID: rant below: Can I also note that if you coverslipped your slides aqueously, you CANNOT remove the coverslips with xylene. Our newest lab tech tried that. Her reasoning was that the sections were paraffin, though she had de-paraffinized them in xylene-EtOH-water steps and kept them aqueous. Explaining why she can't use xylene didn't help. The next time she says she has eight years of experience, I think I'll bring this mishap up. Sigh. Emily -- Yog-Sothoth knows the gate. Yog-Sothoth is the gate. Yog-Sothoth is the key and guardian of the gate. Past, present, future, all are one in Yog-Sothoth. He knows where the Old Ones broke through of old, and where They shall break through again. From b-frederick <@t> northwestern.edu Fri Oct 19 08:58:12 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Oct 19 08:58:22 2007 Subject: [Histonet] removing a coverslip In-Reply-To: Message-ID: <000701c81258$19582c10$d00f7ca5@lurie.northwestern.edu> If you have really old slides (like the glue is drying out and yellowish) you can spray them with histofreeze, slip the edge of a scalpel under the edge of the coverslip and pop it off. Just wipe the condensate form the histofreeze off before you go into xylene to remove the excess mounting media. I spray both sides. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Friday, October 19, 2007 8:22 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] removing a coverslip rant below: Can I also note that if you coverslipped your slides aqueously, you CANNOT remove the coverslips with xylene. Our newest lab tech tried that. Her reasoning was that the sections were paraffin, though she had de-paraffinized them in xylene-EtOH-water steps and kept them aqueous. Explaining why she can't use xylene didn't help. The next time she says she has eight years of experience, I think I'll bring this mishap up. Sigh. Emily -- Yog-Sothoth knows the gate. Yog-Sothoth is the gate. Yog-Sothoth is the key and guardian of the gate. Past, present, future, all are one in Yog-Sothoth. He knows where the Old Ones broke through of old, and where They shall break through again. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Fri Oct 19 09:11:06 2007 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Oct 19 09:14:30 2007 Subject: [Histonet] Mats for the histology lab References: <47176B99.3CA1.003C.0@hollandhospital.org> Message-ID: <5F31F38C96781A4FBE3196EBC22D47802E1179@fhosxchmb006.ADVENTISTCORP.NET> Lab Safety Supply has a great selection of mats for every purpose. We used carpet mats but they would not clean up well. We switched to soft rubber mats that can be pressure-washed for certain areas. We eliminated the mats in the cutting area and use scrapers to clean the floor, as well as require everyone to wear non-skid shoes. Janet Bonner, Florida Hospital Orlando ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Lisa Brenner Sent: Thu 10/18/2007 2:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mats for the histology lab Hello, We recently moved into our brand new lab space. We are trying to keep the floors nice which is no easy feat in a histology lab as you all know. My question is what do all you out there recommend for mats for floors by the cutting and embedding stations? We are hoping to find something that protects the floor, is easy to roll your chair on, and doesn't produce static. Any feed back is appreciated. Thanks and have a great day! Lisa Brenner HTL (ASCP) Histology Technical Consultant Holland Hospital phone: (616)394-3184 lisab@hollandhospital.org Confidentiality Notice: The information contained in this e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information, or Protected Health Information as such term is defined under the Health Insurance Portability and Accountability Act of 1996 (HIPAA). Any unauthorized review, use, disclosure, copying or distribution is prohibited and may be unlawful. If you believe you have received this e-mail in error, please contact the sender by reply e-mail and delete all copies of the original message, including attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From rjbuesa <@t> yahoo.com Fri Oct 19 09:36:46 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 19 09:36:50 2007 Subject: [Histonet] Paraffin and Slide disposal. In-Reply-To: <2842DC75AE43AA4B92954CFB31781BC1821577@CHW-MSG-301.chw.edu> Message-ID: <856468.86649.qm@web61211.mail.yahoo.com> The blocks can be incinerated. For the slides you could try to contact a recycling companey that probably will accept them as "glass to recycle" (and will be cheaper than a "specialized" company). Ren? J "Heckford, Karen - SMMC-SF" wrote: I have been handed a pretty big mess. We have a lot of old blocks and slides at a archiving company which needs to be disposed of because some date back to 1960. Does anyone know of a company that will come in and dispose of blocks and slides? Any input would be greatly appreciated. Cheers, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathology Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From gu.lang <@t> gmx.at Fri Oct 19 09:39:32 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Oct 19 09:39:40 2007 Subject: AW: [Histonet] FW: PAS/Fungus In-Reply-To: <000301c811b4$3ef099c0$3402a8c0@plab.local> Message-ID: <000301c8125d$dd671d00$6412a8c0@dielangs.at> We use mayers hematoxylin 5 min as counterstain. - only nuclei-stain is perhaps easier to distinguish from the fungi. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Cheri Miller Gesendet: Donnerstag, 18. Oktober 2007 20:25 An: 'Histonet' Betreff: [Histonet] FW: PAS/Fungus Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _____ From: Cheri Miller [mailto:cmiller@physlab.com] Sent: Wednesday, October 17, 2007 10:38 AM To: histonet-bounces@lists.utsouthwestern.edu Subject: PAS/Fungus I have a Path that says our PAS/F is weak. His suggestion is that I do a hematoxilyn counter instead of light green. Anyone else doing this?? Seems to me that the counter stain won't make the PAS reaction any stronger. Our procedure is as follows; .5%Periodic acid 5 mins 2 changes DI water Schiff's 20 mins Running water 10 mins Light green 1 min 95,100,100 and 3 xylene I don't get any complaints from the other 6 pathologist's. Share your wisdom. Thanks Cheri Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Fri Oct 19 09:50:36 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Oct 19 09:50:43 2007 Subject: AW: [Histonet] mouse spleen and Prussian Blue stain In-Reply-To: Message-ID: <000401c8125f$68fb6c80$6412a8c0@dielangs.at> The iron-deposit is due to the old erythrocytes, that are destroyed in the spleen. So my suggestion is, that the elder the spleen is, the more erys are destroyed, the more iron-deposit is to be found. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Jacqui Detmar Gesendet: Donnerstag, 18. Oktober 2007 22:09 An: histonet@lists.utsouthwestern.edu. Betreff: [Histonet] mouse spleen and Prussian Blue stain Hello all. I have a couple of blocks embedded with mouse spleen tissue, one which contains older mouse spleen (6 months old) and one with younger mouse spleen (3 weeks). These tissues are further sub-grouped as coming from mice wildtype for caspase-2 or deleted for caspase-2. I wanted to assess the spleen morphology between the two strains b/c the spleen from the deleted mouse appears to be larger as the mouse is older (i.e. spleen from deleted versus wildtype at 3 weeks is same size). The morphology looked a little wonky, so I did a Prussian Blue stain. I have never done this stain before in mouse spleen and before I go embedding some more tissue, I would like to get some info from people who might already know the answer. The deleted and wildtype spleens from the 3-week-old mice looked similar with respect to Prussian blue stain: very few blue deposits in the tissue. In the older tissue, I saw a great deal of iron deposition in the wildtype spleen but almost no deposition in the deleted spleen. This is opposite to what I hypothesized. I thought the deleted spleen would be more damaged and have a greater amount of blue staining. Now I'm worried that the tissue might have been switched during collection or processing - although the size of the tissue in the block and on the section corresponds to the increased spleen size in the deleted mouse. Does anybody out there know what an elderly mouse spleen should look like after Prussian blue staining? Thanks, Jacqui Detmar, Post-doctoral Fellow Samuel Lunenfeld Research Institute, room 876 Mount Sinai Hospital 600 University Avenue Toronto, ON, Canada M5G 1X5 Tel: 416-586-4800 x2451 Fax: 416-586-8588 Email: detmar@mshri.on.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cbobrowi <@t> mcw.edu Fri Oct 19 09:55:02 2007 From: cbobrowi <@t> mcw.edu (Bobrowitz, Carol) Date: Fri Oct 19 09:55:05 2007 Subject: [Histonet] tamm-horsfall protein Message-ID: <8F78639AC56F4143B267FE5F5A1B92C8F0AE65@guyton.phys.mcw.edu> Hi, I need to locate a source for the antibody tamm-horsfall protein. Does anyone have any experience with this antibody? Any help will be appreciated. Thank you in advance. Carol Ann Bobrowitz Medical College of Wisconsin Physiology - Histology cbobrowi@mcw.edu From gu.lang <@t> gmx.at Fri Oct 19 09:59:25 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Oct 19 09:59:30 2007 Subject: AW: [Histonet] Ventanna using other antibodies In-Reply-To: <8839B08E3ED7364E8CBBD53882C984D50994CB7D@MAILSRV01.midmichigan.net> Message-ID: <000501c81260$a460d930$6412a8c0@dielangs.at> Most of the antibodies we use are not from Ventana. With a new one I make a titration-protocol according to the datasheet, with various concentrations in the Ventana antibody diluent. I run that on positiv controls and look for the best result. Sometimes I have no luck to establish a working protocol. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Teri.Hallada@midmichigan.org Gesendet: Freitag, 19. Oktober 2007 14:57 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Ventanna using other antibodies I was wodering if there are others using antibodies not purchased from Ventanna on the Benchmark and what kind of validation studoes did you do? Teresa Hallada BS, MT/CT (ASCP) Lead Cytotechnologist MidMichigan Health - Gratiot teri.hallada@midmichigan.org 989.463.1101 ext 3423 Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law. The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are herby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited. If you have received this email in error, please advise by immediate reply. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Heather.D.Renko <@t> osfhealthcare.org Fri Oct 19 10:19:48 2007 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Fri Oct 19 10:20:07 2007 Subject: [Histonet] Re: Send out/consult billing charges Message-ID: <40026EDDE64CDA47AB382C52619ACD3C0A09DB79@pmc-rfd-mx01.intranet.osfnet.org> Richard, The only CPT code that I would recommend is 99001 - Handling, conveyance or specimen for transfer from the patient in other than a physician's office to a laboratory (distance may be indicated). The problem with this code however is Medicare does not reimburse for this code. Third party payers tend to follow Medicare's lead in dealing with payment of these codes. The following is from a Medicare publication: B = Payment for covered services are always bundled into payment for other services not specified. There will be no relative value units (RVU) or payment amounts for these codes, and no separate payment is ever made. When these services are covered, payment for them is subsumed by the payment for the services to which they are incident. (Example: a telephone call from a hospital nurse regarding care of a patient.) Under this statement is a list of CPT codes that they will not pay. CPT 99001 is in that list. In some cases in our lab, a patient comes in themselves and picks up slides, so of course there is no charge to the patient in these cases. I don't know how a lab could charge a patient directly for this service. So the bottom line is we absorb the cost of doing this. I would agree with Dr. Raff's position on doing all via fed ex and having a standard flat fee for shipping costs. I sure hope this helps some-Happy Friday! Heather D. Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 Direct: 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From B.Deyarmin <@t> wriwindber.org Fri Oct 19 10:44:52 2007 From: B.Deyarmin <@t> wriwindber.org (Brenda Deyarmin) Date: Fri Oct 19 10:47:43 2007 Subject: [Histonet] New CAP Regulations - Breast Tissue Must BeFixed inFormalin In-Reply-To: <8B08EC394B366D4A895DD5686D6AFE4AE7E938@LP-EXCHVS08.CO.IHC.COM> Message-ID: <7690673B79E70D429A12057919339223AC4A92@wri-xchng.WRIWINDBER.ORG> With all the talk about fixative for Her2 and IHC staining...I was wondering if anyone knows of the effects on the DNA from tissue fixed in a fixative other than formalin. I work in a research institute working mainly on breast tissue. It has recently come to my attention that hospitals that we are associated with are using Pen Fix. We are having a difficult time troubleshooting the ability to perform SNP's from paraffin embedded tissue. The protocols from Affy are set for FFPE tissue and I was thinking it may have something to do with an alternative fixative. Thanks, brenda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lance Erickson Sent: Thursday, October 18, 2007 5:39 PM To: Rene J Buesa; Jones, Laura; Histonet (E-mail) Subject: RE: [Histonet] New CAP Regulations - Breast Tissue Must BeFixed inFormalin There is a new CAP checklist for Anatomic Pathology that was updated on the CAP website October 5th. These changes are effective September 27, 2007. All of the new checklist questions involve Her2 testing both IHC and FISH. And actually there is a question that addresses the use of fixatives other than formalin. Look at the notes section of question ANP 22997 you must validate your method with a minimum of 25 (recommended 25-100)and if you use a fixative other than formalin you must show that the results are concordant with the results from formalin-fixed tissues. The next new question specifically addresses the length of time in fixative. Take a look at these new questions at www.cap.org go to the "accreditation and laboratory improvement" tab under the inspection information click the "inspection checklists" and print off the new AP questions. -Lance, Primary Children's, SLC,UT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, October 18, 2007 3:19 PM To: Jones, Laura; Histonet (E-mail) Subject: Re: [Histonet] New CAP Regulations - Breast Tissue Must Be Fixed inFormalin This CAP (and FDA) regulation is a hindrance in any efforts to eliminate carcinogenic formalin from the histo lab, but has to be followed to be in compliance, or to participate in studies about breast cancer. This fact does not qualify for a special treatment/charge. Ren? J. "Jones, Laura" wrote: Hi all. I am wondering if anyone else out there is NOT using formalin on a routine basis any more, and now must bring it back in order to comply with the new CAP regulation. We routinely process everything in Shandon's Glyofixx. Our Pathologist would like to have breast tissues only submitted in formalin, left overnight to fix, and then process from alcohols on during the next day. He feels that the small biopsies will be our main concern, as these are what we usually send for ER/PR, Her2Neu and FISH. If anyone else is going through this same situation, we'd appreciate hearing how you are handling this. Also, a question from my managers: in this situation, can we charge anything for the "special" treatment of the tissue? My feeling was this it was kind of our "fault" that we had to do this, so there probably could not be a charge, but I told them I'd ask the experts. Thanks to all in advance. Laura _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AGrobe2555 <@t> aol.com Fri Oct 19 10:48:46 2007 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Fri Oct 19 10:49:09 2007 Subject: [Histonet] Looking for a Leitz 1512 User manual Message-ID: Good morning, Does anyone know where I might find a Leitz 1512 user/owner manual or where I might download one from the net? Thanks, Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital ************************************** See what's new at http://www.aol.com From mickie25 <@t> netzero.net Fri Oct 19 11:01:04 2007 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Fri Oct 19 11:01:11 2007 Subject: [Histonet] Mohs Histology Tech looking for work In-Reply-To: <8B08EC394B366D4A895DD5686D6AFE4AE7E938@LP-EXCHVS08.CO.IHC.COM> References: <8E7AD740937B954F947F0DB4467EFEE056E9F2@mail.srhs-pa.org><650091.94464.qm@web61212.mail.yahoo.com> <8B08EC394B366D4A895DD5686D6AFE4AE7E938@LP-EXCHVS08.CO.IHC.COM> Message-ID: Dear Histonetters, I have an experienced Mohs histotech who lives in the Memphis, TN area who is looking for work. Downsizing caused her to be relegated to medical assisting leaving the other Mohs tech with seniority to do Mohs, so she left. She is willing to drive as much as 45 minutes for work. She might also be willing to relocate. If you are looking for a 4 year experienced Mohs tech, give me a shout. Thank you. Mickie Mohs Lab Staffing is dedicated to finding jobs for techs and techs for jobs. Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net From jdmd77 <@t> hotmail.com Fri Oct 19 11:06:10 2007 From: jdmd77 <@t> hotmail.com (Julia Dahl) Date: Fri Oct 19 11:06:31 2007 Subject: [Histonet] Charging for patient slide send out In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F3F7D@sjhaexc02.sjha.org> References: <4717537C02000077000089BD@gwmail4.harthosp.org> <1CD6831EB9B26D45B0A3EAA79F7EBD32048F3F7D@sjhaexc02.sjha.org> Message-ID: As I've chimed in before - it's also a loss for the consulting pathologist. Insurances typically pay for about 50% of the consultations that Mosaic GI Pathology sees - at an a rate significantly below the usual payment for the primary read... hence why consultants are switching to billing the institution. This certainly needs to be addressed on a broader scale. An 88321 reimbursement should cover expenses for shipping TO the consultant, FROM the consultant back to the originating institution, overhead for handling the incoming case, report distribution and for the consultation. The average reimbursement that we receive covers little more than shipping and administrative expenses. Julia Dahl MD Consulting GI Pathologist > Date: Thu, 18 Oct 2007 12:45:04 -0400 > From: JWEEMS@sjha.org > To: Rcartun@harthosp.org; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Charging for patient slide send out > CC: > > We have patient pay shipping charge and have facilities that will bill pt ins for second opinion. So many consultants are changing to bill client only that it has really become a problem. j > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 404-851-7376 - Phone > 404-851-7831 - Fax > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Richard > Cartun > Sent: Thursday, October 18, 2007 12:37 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Charging for patient slide send out > > > I know we have discussed this before, but how many of you in clinical pathology labs are doing direct patient billing (cash or credit card) for sending out patient slides for second opinions? We are getting so many requests now we cannot keep up, and we certainly can't afford to do this for free. At a minimum, I think the patient should pay the mailing charge. Thank you. > > Richard > > Richard W. Cartun, Ph.D. > Director, Immunopathology & Histology > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 > (860) 545-0174 Fax > > Confidentiality Notice > > This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _________________________________________________________________ Climb to the top of the charts!? Play Star Shuffle:? the word scramble challenge with star power. http://club.live.com/star_shuffle.aspx?icid=starshuffle_wlmailtextlink_oct From jennifer.l.hofecker <@t> Vanderbilt.Edu Fri Oct 19 11:17:20 2007 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Fri Oct 19 11:17:24 2007 Subject: [Histonet] changing processor reagents In-Reply-To: <168698.66018.qm@web61213.mail.yahoo.com> Message-ID: <898D946569A27444B65667A49C074052013D73CC@mailbe06.mc.vanderbilt.edu> Ren? makes a good point, as always. I would like to add one thing, though. We do not process a high number of blocks in our lab; however, we process nearly every day. We change our cleaning reagents (xylene/alcohol) every 5 runs, no matter how many blocks we've done. The same amount of reagent is pumped in and out of the retort, independent of block counts. I feel safer that way. If you are using a xylene substitute or in some cases, recycled xylene, you may find increasing the frequency of changing the cleaning reagents alone helps you. You can always check alcohols with a hydrometer. Another consideration is the H20 flush, if you have formalin on your processor. Although, the Excelsior is so advanced, maybe it doesn't even need that? I am leery of letting it go to long. If I have to empty a container to run a flush, I might as well change the reagent. Call me paranoid, but I'd rather err on the side of caution. If you looked at the number of cassettes we processed, we'd change the machine every couple of months! I am not willing to try that with a patient's biopsy. As long as we are under budget for reagents, we'll go by number of runs. Just my couple of cents. A different perspective, if you will. Good luck! Have a great weekend. Jennifer L. Hofecker, HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph. (615)343-0083 fax. (615)343-7089 NSH Quality Control Committee Chair -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, October 18, 2007 4:13 PM To: Kinsley, David; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] changing processor reagents Using "runs" as a criteria is not very objective. Use the number of cassettes. I always rotated some reagents/discarded the oldest, every time the TP capacity was reached. Ren? J. "Kinsley, David" wrote: Hi, I would like to know what criteria people are using to determine when to change or rotate reagents on their tissue processors. Is there a certain # of cassettes processed, or # of processing runs, or other criteria? Do you take into consideration the type of tissue processed? We are using a Thermo-Shandon Excelsior, and find that the reagent quality monitor is not a reliable tool for assessing reagent quality and are looking to establish some new guidelines for reagent rotation. Any advice is appreciated. Thanks Dave. ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmerriam2003 <@t> yahoo.com Fri Oct 19 11:47:16 2007 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Fri Oct 19 11:47:21 2007 Subject: [Histonet] NSH and Halloween Message-ID: <528656.59640.qm@web50305.mail.re2.yahoo.com> Are any of the vendors having a Halloween party at the NSH? Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From jqb7 <@t> CDC.GOV Fri Oct 19 11:49:48 2007 From: jqb7 <@t> CDC.GOV (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Fri Oct 19 11:50:07 2007 Subject: [Histonet] NSH and Halloween In-Reply-To: <528656.59640.qm@web50305.mail.re2.yahoo.com> References: <528656.59640.qm@web50305.mail.re2.yahoo.com> Message-ID: <34BB307EFC9A65429BBB49E330675F72045E20AC@LTA3VS003.ees.hhs.gov> Aren't most of us scary enough already? Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Friday, October 19, 2007 12:47 PM To: Histonet Subject: [Histonet] NSH and Halloween Are any of the vendors having a Halloween party at the NSH? Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From igor.deyneko <@t> gmail.com Fri Oct 19 12:34:34 2007 From: igor.deyneko <@t> gmail.com (Igor Deyneko) Date: Fri Oct 19 12:34:39 2007 Subject: [Histonet] Mouse Skin Processing Message-ID: <35e16a770710191034o6ad47ba4x325a1c541e88b48e@mail.gmail.com> Dear Histonetters! I have been havins some problems with processing mouse skin. The blacks are difficult to section, then tissue starts flowing away from the paraffin and the sections do not retain Hematoxylin stain. That suggest to me that the processing and paraffin infiltration were poor. Here's the processing protocol I used for pieces no larger than 1cm^2. 70 % Alcohol - 1.5 hr 70 % Alcohol - 1.5 hr 70 % Alcohol - 1.0 hr 80 % Alcohol - 1.0 hr 95 % Alcohol - 1.0 hr 95 % Alcohol - 1.0 hr 100 % Alcohol - 1.5 hr 100 % Alcohol - 1.5 hr( Due to machine only having 2 stations for that alcohol) Xylene - 1.5 hr Xylene - 1.5 hr(Due to machine having only 2 stations for Xylene) Paraffin - 1.0 hr Paraffin - 1.0 hr Paraffin - 1.0 hr Any suggestions would be greately appreciated. Thank you. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA. From kmerriam2003 <@t> yahoo.com Fri Oct 19 12:43:33 2007 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Fri Oct 19 12:43:38 2007 Subject: [Histonet] Mouse Skin Processing Message-ID: <261903.43336.qm@web50304.mail.re2.yahoo.com> Igor, These processing times might be too long, I would try closer to 30-35 minutes per station for mouse skins. Kim Merriam, MA, HT(ASCP) Cambridge, MA ----- Original Message ---- From: Igor Deyneko To: Histonet@lists.utsouthwestern.edu Sent: Friday, October 19, 2007 1:34:34 PM Subject: [Histonet] Mouse Skin Processing Dear Histonetters! I have been havins some problems with processing mouse skin. The blacks are difficult to section, then tissue starts flowing away from the paraffin and the sections do not retain Hematoxylin stain. That suggest to me that the processing and paraffin infiltration were poor. Here's the processing protocol I used for pieces no larger than 1cm^2. 70 % Alcohol - 1.5 hr 70 % Alcohol - 1.5 hr 70 % Alcohol - 1.0 hr 80 % Alcohol - 1.0 hr 95 % Alcohol - 1.0 hr 95 % Alcohol - 1.0 hr 100 % Alcohol - 1.5 hr 100 % Alcohol - 1.5 hr( Due to machine only having 2 stations for that alcohol) Xylene - 1.5 hr Xylene - 1.5 hr(Due to machine having only 2 stations for Xylene) Paraffin - 1.0 hr Paraffin - 1.0 hr Paraffin - 1.0 hr Any suggestions would be greately appreciated. Thank you. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From jcline <@t> wchsys.org Fri Oct 19 12:46:46 2007 From: jcline <@t> wchsys.org (Joyce Cline) Date: Fri Oct 19 12:46:59 2007 Subject: [Histonet] Histotechs In-Reply-To: Message-ID: <002001c81278$06811f00$1d2a14ac@wchsys.org> We do between 150 to 200 blocks M-F. Starting shift is 6:00am, ending shift 4:30pm. Stain - 1 tech Sp.St - 1 tech Embedding - 1 tech, Coverslips and cuts when through embedding Microtomy - 3 techs that also cover all the other benches. Techs are 3 full time and 1 part time. I have an accessioning assistant that handles all specimens and standing with the pathologist. Bench assignments vary each week. IHC is done by machine. Specials are 3/4 by machine and 1/4 by hand. We cover slip by hand, H&E by machine. We also do two microwave runs a day. We also recycle. I work the bench to cover personnel shortages. (which is quite often since we have all worked here a long time!) We have 4 pathologists and average around 70,000 slides a year. ________________________________________________________________________ ___ Subject: [Histonet] Histotechs Hi there!! Would like to know how many technicians each of you have working on: - stain - special stain - embedding - microtomy We have almost 500 blocks each day, 400 on autotech routine and 100 on microwave routine. We do have 10 technicians that alternates on microtomy (4), embedding (3), stain (1), slides ordenation (1) special stain (same on embedding) and block?s archivation (same on microtomy) and one technician on imunohistochemmistry (that occurs twice a week). We work every day from 7h00 AM to 4h30 PM for 5 pathologists. We have seen labs that has more blocks than us and less technicians.. I do really want to understand how they do that! Tks a lot!! Ms. K?tia Catunda Produ??o +55 12 3203-0612 (direto) +55 12 3203-0633 (PABX) www.cipax.com.br katia.catunda@cipax.com.br -----Mensagem original----- De: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Em nome de Ms. K?tia Cristina Catunda Enviada em: sexta-feira, 19 de outubro de 2007 09:28 Para: 'Kinsley, David'; histonet@lists.utsouthwestern.edu Assunto: RES: [Histonet] changing processor reagents David, Here we have a routine of approximately 450 blocks each day and three tissue processors. We do change the last alcohol and xylene of the carroussel every single day (since the processor has been used). Once a week we change one of the two waxes. Hope it helps Ms. K?tia Catunda Produ??o +55 12 3203-0612 (direto) +55 12 3203-0633 (PABX) www.cipax.com.br katia.catunda@cipax.com.br _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From liz <@t> premierlab.com Fri Oct 19 12:55:02 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Oct 19 12:55:08 2007 Subject: [Histonet] CD166 control and staining pattern Message-ID: I need some help with choosing a CD166 control, the protocol states to use human skin which I did and I have some staining in the epithelium to me looks like dentritic cells. If there is anyone out there that can help me with for both control tissue and localization Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 From b-frederick <@t> northwestern.edu Fri Oct 19 13:38:33 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Oct 19 13:38:16 2007 Subject: [Histonet] NSH and Halloween In-Reply-To: <34BB307EFC9A65429BBB49E330675F72045E20AC@LTA3VS003.ees.hhs.gov> Message-ID: <000301c8127f$4413bd30$d00f7ca5@lurie.northwestern.edu> I will at least have my Halloweeen sweater with me. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Friday, October 19, 2007 11:50 AM To: Kim Merriam; Histonet Subject: RE: [Histonet] NSH and Halloween Aren't most of us scary enough already? Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Friday, October 19, 2007 12:47 PM To: Histonet Subject: [Histonet] NSH and Halloween Are any of the vendors having a Halloween party at the NSH? Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mari.ann.mailhiot <@t> leica-microsystems.com Fri Oct 19 14:20:09 2007 From: mari.ann.mailhiot <@t> leica-microsystems.com (mari.ann.mailhiot@leica-microsystems.com) Date: Fri Oct 19 14:20:17 2007 Subject: [Histonet] Looking for a Leitz 1512 User manual In-Reply-To: Message-ID: Hi Albert I will be glad tosend you a copy by email. Best Regards Mari Ann Mailhiot BA HT ASCP Application Specialist Leica Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From sbreeden <@t> nmda.nmsu.edu Fri Oct 19 14:31:22 2007 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri Oct 19 14:31:27 2007 Subject: [Histonet] OT: Joe the Toe Party Message-ID: <4D14F0FC9316DD41972D5F03C070908B8F4708@nmdamailsvr.nmda.ad.nmsu.edu> Where's the "Meet JTT" reception at NSH? And will he be giving autographs? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From slappycraw <@t> yahoo.com Fri Oct 19 14:32:15 2007 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Fri Oct 19 14:32:20 2007 Subject: [Histonet] Mouse Skin Processing In-Reply-To: <261903.43336.qm@web50304.mail.re2.yahoo.com> Message-ID: <988832.59154.qm@web53603.mail.re2.yahoo.com> I would shorten some of the alcohol stations, currently you've got about 10 hours in alcohol, seems like too much IMO. Kim Merriam wrote: Igor, These processing times might be too long, I would try closer to 30-35 minutes per station for mouse skins. Kim Merriam, MA, HT(ASCP) Cambridge, MA ----- Original Message ---- From: Igor Deyneko To: Histonet@lists.utsouthwestern.edu Sent: Friday, October 19, 2007 1:34:34 PM Subject: [Histonet] Mouse Skin Processing Dear Histonetters! I have been havins some problems with processing mouse skin. The blacks are difficult to section, then tissue starts flowing away from the paraffin and the sections do not retain Hematoxylin stain. That suggest to me that the processing and paraffin infiltration were poor. Here's the processing protocol I used for pieces no larger than 1cm^2. 70 % Alcohol - 1.5 hr 70 % Alcohol - 1.5 hr 70 % Alcohol - 1.0 hr 80 % Alcohol - 1.0 hr 95 % Alcohol - 1.0 hr 95 % Alcohol - 1.0 hr 100 % Alcohol - 1.5 hr 100 % Alcohol - 1.5 hr( Due to machine only having 2 stations for that alcohol) Xylene - 1.5 hr Xylene - 1.5 hr(Due to machine having only 2 stations for Xylene) Paraffin - 1.0 hr Paraffin - 1.0 hr Paraffin - 1.0 hr Any suggestions would be greately appreciated. Thank you. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Larry A. Woody Amgen Seattle, Wa. __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From b-frederick <@t> northwestern.edu Fri Oct 19 15:06:28 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Oct 19 15:06:40 2007 Subject: [Histonet] OT: Joe the Toe Party In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B8F4708@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <000501c8128b$8bdd0110$d00f7ca5@lurie.northwestern.edu> Let me know when this happens, I'd like to meet JTT also. Will he be bringing his fire extinguisher? Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Friday, October 19, 2007 2:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] OT: Joe the Toe Party Where's the "Meet JTT" reception at NSH? And will he be giving autographs? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vazquezr <@t> ohsu.edu Fri Oct 19 16:27:17 2007 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Oct 19 16:27:50 2007 Subject: [Histonet] OT: Joe the Toe Party Message-ID: He will be bringing his ear protectors and toe nail clips...pedicure anyone?? Happy Friday!!! ;) Robyn From jnocito <@t> satx.rr.com Fri Oct 19 18:27:51 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Oct 19 18:27:56 2007 Subject: [Histonet] OT: Joe the Toe Party References: Message-ID: <000c01c812a7$ac0b28b0$0302a8c0@yourxhtr8hvc4p> I all want you to know that I haven't clip my toenails in months just for this special occasion. We can have clipping party and do PAS/Fungus all night long. no, no. Don't thank me, it's my pleasure. ----- Original Message ----- From: "Robyn Vazquez" To: ; ; Sent: Friday, October 19, 2007 4:27 PM Subject: RE: [Histonet] OT: Joe the Toe Party > He will be bringing his ear protectors and toe nail clips...pedicure > anyone?? Happy Friday!!! ;) > Robyn > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From godsgalnow <@t> aol.com Fri Oct 19 18:49:24 2007 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Fri Oct 19 18:49:35 2007 Subject: [Histonet] prostate Message-ID: <8C9E0C1D0322D5B-820-1CE0@FWM-D07.sysops.aol.com> Is anyone using the following antibodies for prostate cancer?? Prognostically, I mean. cox2 nf kb s100a4 akt Thanks in advance Roxanne Soto HT(ASCP)QIHC ________________________________________________________________________ Email and AIM finally together. You've gotta check out free AOL Mail! - http://mail.aol.com From jnocito <@t> satx.rr.com Fri Oct 19 19:20:16 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Oct 19 19:20:22 2007 Subject: [Histonet] NSH and Halloween References: <528656.59640.qm@web50305.mail.re2.yahoo.com> <34BB307EFC9A65429BBB49E330675F72045E20AC@LTA3VS003.ees.hhs.gov> Message-ID: <001701c812ae$fdd89c20$0302a8c0@yourxhtr8hvc4p> I'm going to dress up like a Boston Red Sox. Now, that's scary ----- Original Message ----- From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" To: "Kim Merriam" ; "Histonet" Sent: Friday, October 19, 2007 11:49 AM Subject: RE: [Histonet] NSH and Halloween Aren't most of us scary enough already? Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Friday, October 19, 2007 12:47 PM To: Histonet Subject: [Histonet] NSH and Halloween Are any of the vendors having a Halloween party at the NSH? Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Fri Oct 19 19:25:39 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Oct 19 19:25:44 2007 Subject: [Histonet] Paraffin and Slide disposal. References: <2842DC75AE43AA4B92954CFB31781BC1821577@CHW-MSG-301.chw.edu> Message-ID: <005c01c812af$be41cfe0$0302a8c0@yourxhtr8hvc4p> Karen, who ever (whom ever?) takes away your hazardous waste should be able to take it away. JTT ----- Original Message ----- From: "Heckford, Karen - SMMC-SF" To: Sent: Friday, October 19, 2007 6:59 AM Subject: [Histonet] Paraffin and Slide disposal. >I have been handed a pretty big mess. We have a lot of old blocks and > slides at a archiving company which needs to be disposed of because some > date back to 1960. Does anyone know of a company that will come in and > dispose of blocks and slides? Any input would be greatly appreciated. > Cheers, > > Karen Heckford HT (ASCP) CE > Lead Histology Technician > Histology/Pathology Department > St. Mary's Medical Center > 450 Stanyan St. > San Francisco, Ca. 94117 > 415-668-1000 ext. 6167 > Fax: 415-750-8123 > email: kheckfor@chw.edu > -------------------------------------------------------------------------------- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From christiegowan <@t> msn.com Fri Oct 19 15:15:46 2007 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Fri Oct 19 19:52:36 2007 Subject: [Histonet] OT: Joe the Toe Party In-Reply-To: <000501c8128b$8bdd0110$d00f7ca5@lurie.northwestern.edu> Message-ID: Joe, Looks like you have a fan club! christie >From: "Bernice Frederick" >To: "'Breeden, Sara'" >, >Subject: RE: [Histonet] OT: Joe the Toe Party >Date: Fri, 19 Oct 2007 15:06:28 -0500 > >Let me know when this happens, I'd like to meet JTT also. Will he be >bringing his fire extinguisher? >Bernice > >Bernice Frederick HTL (ASCP) >Northwestern University >Pathology Core Facility >710 N Fairbanks Court >Olson 8-421 >Chicago,IL 60611 >312-503-3723 > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, >Sara >Sent: Friday, October 19, 2007 2:31 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] OT: Joe the Toe Party > >Where's the "Meet JTT" reception at NSH? And will he be giving >autographs? > > > >Sally Breeden, HT(ASCP) > >NM Dept. of Agriculture > >Veterinary Diagnostic Services > >PO Box 4700 > >Albuquerque, NM 87106 > >505-841-2576 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sccrshlly <@t> yahoo.com Fri Oct 19 23:02:48 2007 From: sccrshlly <@t> yahoo.com (Shelly Coker) Date: Fri Oct 19 23:02:53 2007 Subject: [Histonet] Histotechs Message-ID: <697961.85412.qm@web90306.mail.mud.yahoo.com> We currently have 2 techs. We do 100-150 block per day. We do start to finish on these blocks: gross, processing (microwave), cutting, embedding, and special stains (not very many different stains, though). The only other person in the lab is an assistant for about 2 hours each day to help with paperwork. Perhaps look at your work flow. Three people on embedding all the time with only 4 cutters seems like a mismatch. As does having three people doing special stains. I recently worked at a much larger operation (1400-1600 blocks per day) and we managed with 2 embedders usually and 8-10 cutters, one of which got up halfway through the day to do the special stains. One thing that may help you is a lab assistant to pick up slides, help with coverslipping and handing out slides, answering phones, filing blocks and slides. These are tasks that the tech doesn't HAVE to do. Think of how much tech time you could save for actual tech work by simply delegating some of the non-technical duties to a lab assistant. Just food for thought! Shelly __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From rjbuesa <@t> yahoo.com Sat Oct 20 07:33:56 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Oct 20 07:34:00 2007 Subject: [Histonet] Histotechs In-Reply-To: <697961.85412.qm@web90306.mail.mud.yahoo.com> Message-ID: <810398.91917.qm@web61219.mail.yahoo.com> Amen! Ren? J. Shelly Coker wrote: We currently have 2 techs. We do 100-150 block per day. We do start to finish on these blocks: gross, processing (microwave), cutting, embedding, and special stains (not very many different stains, though). The only other person in the lab is an assistant for about 2 hours each day to help with paperwork. Perhaps look at your work flow. Three people on embedding all the time with only 4 cutters seems like a mismatch. As does having three people doing special stains. I recently worked at a much larger operation (1400-1600 blocks per day) and we managed with 2 embedders usually and 8-10 cutters, one of which got up halfway through the day to do the special stains. One thing that may help you is a lab assistant to pick up slides, help with coverslipping and handing out slides, answering phones, filing blocks and slides. These are tasks that the tech doesn't HAVE to do. Think of how much tech time you could save for actual tech work by simply delegating some of the non-technical duties to a lab assistant. Just food for thought! Shelly __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From jnocito <@t> satx.rr.com Sat Oct 20 09:35:50 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Sat Oct 20 09:35:55 2007 Subject: [Histonet] OT: Joe the Toe Party References: Message-ID: <000701c81326$83c749e0$0302a8c0@yourxhtr8hvc4p> Yes, but is it a club or a cult, being Halloween and all? ----- Original Message ----- From: "CHRISTIE GOWAN" To: ; ; Sent: Friday, October 19, 2007 3:15 PM Subject: RE: [Histonet] OT: Joe the Toe Party > Joe, > Looks like you have a fan club! > christie > > >>From: "Bernice Frederick" >>To: "'Breeden, Sara'" >>, >>Subject: RE: [Histonet] OT: Joe the Toe Party >>Date: Fri, 19 Oct 2007 15:06:28 -0500 >> >>Let me know when this happens, I'd like to meet JTT also. Will he be >>bringing his fire extinguisher? >>Bernice >> >>Bernice Frederick HTL (ASCP) >>Northwestern University >>Pathology Core Facility >>710 N Fairbanks Court >>Olson 8-421 >>Chicago,IL 60611 >>312-503-3723 >> >> >>-----Original Message----- >>From: histonet-bounces@lists.utsouthwestern.edu >>[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, >>Sara >>Sent: Friday, October 19, 2007 2:31 PM >>To: histonet@lists.utsouthwestern.edu >>Subject: [Histonet] OT: Joe the Toe Party >> >>Where's the "Meet JTT" reception at NSH? And will he be giving >>autographs? >> >> >> >>Sally Breeden, HT(ASCP) >> >>NM Dept. of Agriculture >> >>Veterinary Diagnostic Services >> >>PO Box 4700 >> >>Albuquerque, NM 87106 >> >>505-841-2576 >> >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From froyer <@t> bitstream.net Sat Oct 20 11:14:40 2007 From: froyer <@t> bitstream.net (Ford Royer) Date: Sat Oct 20 11:15:49 2007 Subject: [Histonet] OT: Joe the Toe Party In-Reply-To: <000701c81326$83c749e0$0302a8c0@yourxhtr8hvc4p> References: <000701c81326$83c749e0$0302a8c0@yourxhtr8hvc4p> Message-ID: <005101c81334$51fd48c0$0200a8c0@yoursz6x6sefxo> >From some of the flames you've received in the past, it is positively a Club... about 42" long with nails sticking out the end. Along with your asbestos underwear, remember to bring your football (or batting) helmet. ;-) Sorry I won't be able to attend this year. Too many preexisting conflicting commitments. Have a wonderful time everyone. ~ Ford Ford M. Royer, MT(ASCP) Minnesota Medical Minneapolis, MN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Saturday, October 20, 2007 9:36 AM To: CHRISTIE GOWAN; b-frederick@northwestern.edu; sbreeden@nmda.nmsu.edu; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] OT: Joe the Toe Party Yes, but is it a club or a cult, being Halloween and all? From detmar <@t> mshri.on.ca Sat Oct 20 12:32:58 2007 From: detmar <@t> mshri.on.ca (Jacqui Detmar) Date: Sat Oct 20 12:33:14 2007 Subject: [Histonet] fixative recommendation Message-ID: Hi all. I am posting this question on behalf of a colleague. She would like to start archiving human placenta tissue and was wondering what would be an ideal fixative. Any help would be appreciated. Also, she asked me for my opinion on a product called "HOPE" fixative, which is produced by Polysciences, but I have not used this fixative. I was hoping someone out there in histoland has experience with this fixative and would give me his/her opinion. Lastly, if anybody has any helpful suggestions or advice to give re: setting up archives of tissue, please pass this on. Fore-warned is fore-armed, eh? Thanks in advance, Jacqui Detmar, Post-doctoral Fellow Samuel Lunenfeld Research Institute, room 876 Mount Sinai Hospital 600 University Avenue, Toronto, ON, Canada M5G 1X5 Tel: 416-586-4800 x2451/x2290 Fax: 416-586-8588 email: detmar@mshri.on.ca From pruegg <@t> ihctech.net Sat Oct 20 13:13:45 2007 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Sat Oct 20 13:13:57 2007 Subject: [Histonet] NSH and Halloween In-Reply-To: <001701c812ae$fdd89c20$0302a8c0@yourxhtr8hvc4p> References: <528656.59640.qm@web50305.mail.re2.yahoo.com><34BB307EFC9A65429BBB49E330675F72045E20AC@LTA3VS003.ees.hhs.gov> <001701c812ae$fdd89c20$0302a8c0@yourxhtr8hvc4p> Message-ID: <005c01c81344$f564b1a0$0202a8c0@ihctechq9h2qof> If you are coming to Denver in what we call "Rocktober" dressed as a Red Sox or Cleveland Indian, you would be taking your life into your hands. Go Rockies! Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Friday, October 19, 2007 6:20 PM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Kim Merriam; Histonet Subject: Re: [Histonet] NSH and Halloween I'm going to dress up like a Boston Red Sox. Now, that's scary ----- Original Message ----- From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" To: "Kim Merriam" ; "Histonet" Sent: Friday, October 19, 2007 11:49 AM Subject: RE: [Histonet] NSH and Halloween Aren't most of us scary enough already? Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Friday, October 19, 2007 12:47 PM To: Histonet Subject: [Histonet] NSH and Halloween Are any of the vendors having a Halloween party at the NSH? Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sat Oct 20 13:14:24 2007 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Sat Oct 20 13:14:33 2007 Subject: [Histonet] prostate In-Reply-To: <8C9E0C1D0322D5B-820-1CE0@FWM-D07.sysops.aol.com> References: <8C9E0C1D0322D5B-820-1CE0@FWM-D07.sysops.aol.com> Message-ID: <005d01c81345$0c60c9c0$0202a8c0@ihctechq9h2qof> I have used pAKT. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: Friday, October 19, 2007 5:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] prostate Is anyone using the following antibodies for prostate cancer?? Prognostically, I mean. cox2 nf kb s100a4 akt Thanks in advance Roxanne Soto HT(ASCP)QIHC ________________________________________________________________________ Email and AIM finally together. You've gotta check out free AOL Mail! - http://mail.aol.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dahui_you <@t> yahoo.com Sat Oct 20 21:23:32 2007 From: dahui_you <@t> yahoo.com (Dahui You) Date: Sat Oct 20 21:23:36 2007 Subject: [Histonet] CD3 antibody Message-ID: <665746.38567.qm@web35713.mail.mud.yahoo.com> Hello, everyone: We are trying to identify CD3 positive T cells and/or H1N1 influenza in some HistoChoice (Amresco) fixed and paraffin embedded mouse lung sections. Does anybody have experience with these antibodies? Any suggestions on companies and antibodies? Thanks in advance and I appreciate your help. Dahui Dahui You Department of Biological Sciences Louisiana State University Baton Rouge, LA 70803 Tel:225-578-5960 Fax:225-578-2594 __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From kb4091 <@t> yahoo.com Sun Oct 21 07:17:12 2007 From: kb4091 <@t> yahoo.com (Kathy Munro) Date: Sun Oct 21 07:17:23 2007 Subject: [Histonet] Pricing for histologyt services Message-ID: <735379.23922.qm@web39601.mail.mud.yahoo.com> Has anyone out there ever priced out what it costs to do the following CPT codes? Please let me know if you have. I could use any help I can get on this. My supervisor wants from beginning to end ..... 88104 cyto smears 88108 cytospins 88173 cyto FNA 88302 Level II 88305 Level III 88307 Level IV 88309 Level V 88311 Decal 88312 Spec stains group 1 88313 Special stains Group 2 Any help would be greatly appreciated!! Thank you in adavance Kathy Bowden kb4091@yahoo.com __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From rjbuesa <@t> yahoo.com Sun Oct 21 07:59:52 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Oct 21 08:00:06 2007 Subject: [Histonet] CD3 antibody In-Reply-To: <665746.38567.qm@web35713.mail.mud.yahoo.com> Message-ID: <899901.8328.qm@web61211.mail.yahoo.com> I used CD-3,T antibody from DAKO (polyclonal rabbit IgG) at 1:200 dilution, HIER at pH6 incubated for 30 minutes at RT and standard detection systems. Tonsil as control. Ren? J. Dahui You wrote: Hello, everyone: We are trying to identify CD3 positive T cells and/or H1N1 influenza in some HistoChoice (Amresco) fixed and paraffin embedded mouse lung sections. Does anybody have experience with these antibodies? Any suggestions on companies and antibodies? Thanks in advance and I appreciate your help. Dahui Dahui You Department of Biological Sciences Louisiana State University Baton Rouge, LA 70803 Tel:225-578-5960 Fax:225-578-2594 __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From pruegg <@t> ihctech.net Sun Oct 21 11:11:03 2007 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Sun Oct 21 11:06:50 2007 Subject: [Histonet] CD3 antibody In-Reply-To: <899901.8328.qm@web61211.mail.yahoo.com> Message-ID: <200710211606.l9LG6bEW009192@pro12.abac.com> I would recommend lab vision rab monoclonal for cd3, haven't tried it with alcohol based fixatives but it works great with formalin fixed tissues for many species, human, dog, rat, mouse, etc. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Sunday, October 21, 2007 7:00 AM To: Dahui You; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] CD3 antibody I used CD-3,T antibody from DAKO (polyclonal rabbit IgG) at 1:200 dilution, HIER at pH6 incubated for 30 minutes at RT and standard detection systems. Tonsil as control. Ren? J. Dahui You wrote: Hello, everyone: We are trying to identify CD3 positive T cells and/or H1N1 influenza in some HistoChoice (Amresco) fixed and paraffin embedded mouse lung sections. Does anybody have experience with these antibodies? Any suggestions on companies and antibodies? Thanks in advance and I appreciate your help. Dahui Dahui You Department of Biological Sciences Louisiana State University Baton Rouge, LA 70803 Tel:225-578-5960 Fax:225-578-2594 __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Sun Oct 21 13:02:12 2007 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sun Oct 21 13:02:18 2007 Subject: [Histonet] Tris buffer Message-ID: <582736990710211102w460aa022ja88f520ba4deccf1@mail.gmail.com> Sheila, The idea is to make sure the environment the sections are in before adding the antibodies is as similar to the environment the antibodies themselves are in as possible. This increases the avidity and affinity of the antibodies for the antigens. Think of it like a lock and key. If your hand is shaking you won't be able to get the key into the lock as well. The molecules of the proteins are doing the same thing when they are of a different pH (or temperature for that matter). When the molecules aren't moving around or twisting (like what happens in antigen retrieval) they can line up and the reactive parts can get close enough to bond properly. Amos Brooks From massimo.tosi.ajoj <@t> alice.it Sun Oct 21 18:24:12 2007 From: massimo.tosi.ajoj <@t> alice.it (Massimo Tosi) Date: Sun Oct 21 18:24:30 2007 Subject: [Histonet] Fw: test Message-ID: <000701c81439$7d56c8b0$0c01a8c0@SN300208440005> ----- Original Message ----- From: "Massimo Tosi" To: "histonet" Sent: Monday, October 22, 2007 12:56 AM Subject: test > test di prova From pvalente <@t> sbcglobal.net Sun Oct 21 19:15:40 2007 From: pvalente <@t> sbcglobal.net (Patricia Valente) Date: Sun Oct 21 19:15:44 2007 Subject: [Histonet] Histotech - open position Message-ID: <53798.312.qm@web81701.mail.mud.yahoo.com> We have a vacant position for an experienced Histotech - ASCP and 5 yrs minimum experience preferred. Come join our Urological Pathology team in San Antonio. For more info - E-mail bbailey@uropathllc.com or fax resume to: 210 - 521 - 7710 From pvalente <@t> sbcglobal.net Sun Oct 21 19:15:40 2007 From: pvalente <@t> sbcglobal.net (Patricia Valente) Date: Sun Oct 21 19:15:48 2007 Subject: [Histonet] Histotech - open position Message-ID: <53798.312.qm@web81701.mail.mud.yahoo.com> We have a vacant position for an experienced Histotech - ASCP and 5 yrs minimum experience preferred. Come join our Urological Pathology team in San Antonio. For more info - E-mail bbailey@uropathllc.com or fax resume to: 210 - 521 - 7710 From jkiernan <@t> uwo.ca Sun Oct 21 23:18:59 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Sun Oct 21 23:19:02 2007 Subject: [Histonet] PAS for frozens In-Reply-To: <471BA3B9.11C6.00D6.0@mrl.ubc.ca> References: <471BA3B9.11C6.00D6.0@mrl.ubc.ca> Message-ID: Don't do anything without knowing the reason! Of the four liquids you list, the first three will extract most of the lipids (PAS +ve and others) from the sections. Formalin will not do this (unless it's diluted with a large excess of an organic solvent such as alcohol). You didn't say if the pieces of lung had been fixed before freezing. If not, brief treatment with formalin won't fix them, though it will probably reduce autolysis and delay putrefaction. The three lipid solvents that you list will all rapidly provide good fixation at the micro-anatomical level (e.g. X40 dry objective), as long as you're not interested in lipids or enzyme activity histochemistry. John Kiernan Anatomy, UWO London, Canada --- ----- Original Message ----- From: Mark Elliott Date: Sunday, October 21, 2007 22:09 Subject: Re: [Histonet] PAS for frozens To: John Kiernan > Thanks very much for the info, it is much appreciated. > Have received a number of methods with various pre-fixes-none, > acetic ethanol, ethanol, Carnoys and formalin. Will try a > variety. > Mark > > >>> John Kiernan 10/18/2007 8:22 AM >>> > If your frozen sections are mounted on slides, the periodic acid- > Schiff PAS procedure is the same as for paraffin sections. > Lipids, especially hydrophilic ones, are PAS-positive to a > variable extent. This is partly on account of the sugars in > glycolipids and partly due to atmospheric oxidation at > unsaturated bonds. > > ----- Original Message ----- > From: Mark Elliott > Date: Tuesday, October 16, 2007 13:58 > Subject: [Histonet] PAS for frozens > To: histonet@lists.utsouthwestern.edu > > > Does anyone have a procedure for doing a PAS on frozen > > tissue?? I assume we need to shorten the times in the > > various regents, but by how much? Any tips/tricks would > be > > greatly appreciated. We are staining human lung tissue. > > > > Thanks > > Mark in dreary Vancouver BC From jkiernan <@t> uwo.ca Sun Oct 21 23:39:23 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Sun Oct 21 23:39:27 2007 Subject: [Histonet] fixative recommendation In-Reply-To: References: Message-ID: The effects of a fixative on a tissue depend on the physical properties and chemical reactivities of the ingredients. The requirements of the investigation must also be considered. What will be done with the archived placental tissue? Every textbook of histotechnology, microtechnique or histochemistry explains how to go about choosing a fixative. Before buying any proprietary fixative mixture with a trade-name, find out the names and concentrations of all the ingredients. Without this information it is impossible to decide whether the mixture will be suitable for your purposes. If you look up the Histonet archives (www.histosearch.com) you will find many warnings of this kind, from several different people. There is no shortage of excellent fixatives of known composition that you can either mix for yourself or buy ready-made. John Kiernan Anatomy, UWO London, Canada --- ----- Original Message ----- From: Jacqui Detmar Date: Saturday, October 20, 2007 13:35 Subject: [Histonet] fixative recommendation To: histonet@lists.utsouthwestern.edu > Hi all. I am posting this question on behalf of a > colleague. She would like to start archiving human > placenta tissue and was wondering what would be an ideal > fixative. Any help would be appreciated. > > Also, she asked me for my opinion on a product called "HOPE" > fixative, which is produced by Polysciences, but I have not used > this fixative. I was hoping someone out there in histoland > has experience with this fixative and would give me his/her > opinion. > > Lastly, if anybody has any helpful suggestions or advice to give > re: setting up archives of tissue, please pass this on. > Fore-warned is fore-armed, eh? > > Thanks in advance, > > > Jacqui Detmar, Post-doctoral Fellow > Samuel Lunenfeld Research Institute, room 876 > Mount Sinai Hospital > 600 University Avenue, > Toronto, ON, Canada > M5G 1X5 > > Tel: 416-586-4800 x2451/x2290 > Fax: 416-586-8588 > email: detmar@mshri.on.ca > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From vhlwong <@t> yahoo.com Mon Oct 22 03:04:24 2007 From: vhlwong <@t> yahoo.com (Victor Wong) Date: Mon Oct 22 03:04:32 2007 Subject: [Histonet] HELP!!! peroxidase blocking on HCC paraffin sections!!!! Message-ID: <47703.20903.qm@web52106.mail.re2.yahoo.com> Hi all, Last time I tried to block endogenous peroxidase with 0.3% hydrogen peroxide for 15 minutes but it came out a lot of background. Then I tried to block with 3% in PBS for 10 and 30 minutes. The background staining was greatly reduced esp. in that treated for 30 minutes. But the positive staining was also muchly reduced. Since I have no spared slides left, I cannot make others trial to massage the blocking conditions. I felt very frustrated and now I am seeking help from you folks. I use the DAKO's Envision+ kit therefore it should not be the endogenous biotin's problem. I also want to know the rationale behind the H2O2 on blocking the peroxide and the visualization of chromogen. Tons of thanks. Victor __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From kappeler <@t> patho.unibe.ch Mon Oct 22 05:26:51 2007 From: kappeler <@t> patho.unibe.ch (Andi Kappeler) Date: Mon Oct 22 05:27:11 2007 Subject: [Histonet] tamm-horsfall protein References: <8F78639AC56F4143B267FE5F5A1B92C8F0AE65@guyton.phys.mcw.edu> Message-ID: <00c101c81496$100aeae0$27955c82@patho.unibe.ch> Hi Carol we use mo-a-THP, clone 10.32, from Cedarlane (http://www.cedarlanelabs.com/DataSheets/CL1032A.PDF). Working dilution 11:200, no pretreatment. Hope this helps. Best regards Andi Kappeler Institute of Pathology, University of Bern, Switzerland ----- Original Message ----- From: "Bobrowitz, Carol" To: Sent: Friday, October 19, 2007 4:55 PM Subject: [Histonet] tamm-horsfall protein Hi, I need to locate a source for the antibody tamm-horsfall protein. Does anyone have any experience with this antibody? Any help will be appreciated. Thank you in advance. Carol Ann Bobrowitz Medical College of Wisconsin Physiology - Histology cbobrowi@mcw.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BMolinari <@t> heart.thi.tmc.edu Mon Oct 22 06:25:32 2007 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Mon Oct 22 06:25:35 2007 Subject: [Histonet] NSH and Halloween/OT world Series In-Reply-To: <005c01c81344$f564b1a0$0202a8c0@ihctechq9h2qof> Message-ID: Is anyone interested in trying to get tickets to one of the games? Red Sox fan on the way. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC 1-283 Houston, TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pruegg@ihctech.net Sent: Saturday, October 20, 2007 1:14 PM To: 'Joe Nocito'; 'Bartlett, Jeanine (CDC/CCID/NCZVED)'; 'Kim Merriam'; 'Histonet' Subject: RE: [Histonet] NSH and Halloween If you are coming to Denver in what we call "Rocktober" dressed as a Red Sox or Cleveland Indian, you would be taking your life into your hands. Go Rockies! Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Friday, October 19, 2007 6:20 PM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Kim Merriam; Histonet Subject: Re: [Histonet] NSH and Halloween I'm going to dress up like a Boston Red Sox. Now, that's scary ----- Original Message ----- From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" To: "Kim Merriam" ; "Histonet" Sent: Friday, October 19, 2007 11:49 AM Subject: RE: [Histonet] NSH and Halloween Aren't most of us scary enough already? Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Friday, October 19, 2007 12:47 PM To: Histonet Subject: [Histonet] NSH and Halloween Are any of the vendors having a Halloween party at the NSH? Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Oct 22 07:53:19 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Oct 22 07:53:23 2007 Subject: [Histonet] HELP!!! peroxidase blocking on HCC paraffin sections!!!! In-Reply-To: <47703.20903.qm@web52106.mail.re2.yahoo.com> Message-ID: <268371.5607.qm@web61225.mail.yahoo.com> Victor: You should be able to quench endogenous peroxidase with 3% UNLESS the H2O2 you are using has "expired" meaning that is NOT really a 3% solution. My first reccommendation would be to get really fresh H2O2 and try, again, Just 5 minutes at 3%, and that ought to be enough. The rationale is that peroxidase is an omnipresent enzime in all cells and if not oxidized (with H2O2) will produce a "background noise" (=unspecific staining) during the IHC procedure. Similarly DAB to react needs the addition of H2O2. Try a new fresh batch of hydrogen peroxide. Ren? J. Victor Wong wrote: Hi all, Last time I tried to block endogenous peroxidase with 0.3% hydrogen peroxide for 15 minutes but it came out a lot of background. Then I tried to block with 3% in PBS for 10 and 30 minutes. The background staining was greatly reduced esp. in that treated for 30 minutes. But the positive staining was also muchly reduced. Since I have no spared slides left, I cannot make others trial to massage the blocking conditions. I felt very frustrated and now I am seeking help from you folks. I use the DAKO's Envision+ kit therefore it should not be the endogenous biotin's problem. I also want to know the rationale behind the H2O2 on blocking the peroxide and the visualization of chromogen. Tons of thanks. Victor __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From b-frederick <@t> northwestern.edu Mon Oct 22 08:12:32 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon Oct 22 08:12:45 2007 Subject: [Histonet] OT: Joe the Toe Party In-Reply-To: <000c01c812a7$ac0b28b0$0302a8c0@yourxhtr8hvc4p> Message-ID: <000501c814ad$37d45860$d00f7ca5@lurie.northwestern.edu> Are you also bringing the Nair to soften those toenails? Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: Friday, October 19, 2007 6:28 PM To: Robyn Vazquez; histonet@lists.utsouthwestern.edu; sbreeden@nmda.nmsu.edu; b-frederick@northwestern.edu Subject: Re: [Histonet] OT: Joe the Toe Party I all want you to know that I haven't clip my toenails in months just for this special occasion. We can have clipping party and do PAS/Fungus all night long. no, no. Don't thank me, it's my pleasure. ----- Original Message ----- From: "Robyn Vazquez" To: ; ; Sent: Friday, October 19, 2007 4:27 PM Subject: RE: [Histonet] OT: Joe the Toe Party > He will be bringing his ear protectors and toe nail clips...pedicure > anyone?? Happy Friday!!! ;) > Robyn > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Mon Oct 22 08:24:31 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Mon Oct 22 08:25:08 2007 Subject: [Histonet] OT: Joe the Toe Party In-Reply-To: <000501c814ad$37d45860$d00f7ca5@lurie.northwestern.edu> References: <000c01c812a7$ac0b28b0$0302a8c0@yourxhtr8hvc4p> <000501c814ad$37d45860$d00f7ca5@lurie.northwestern.edu> Message-ID: Perhaps, more likely to be the good old Jim Bean to soften the senses. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: 22 October 2007 14:13 To: 'Joe Nocito'; 'Robyn Vazquez'; histonet@lists.utsouthwestern.edu; sbreeden@nmda.nmsu.edu Subject: RE: [Histonet] OT: Joe the Toe Party Are you also bringing the Nair to soften those toenails? Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: Friday, October 19, 2007 6:28 PM To: Robyn Vazquez; histonet@lists.utsouthwestern.edu; sbreeden@nmda.nmsu.edu; b-frederick@northwestern.edu Subject: Re: [Histonet] OT: Joe the Toe Party I all want you to know that I haven't clip my toenails in months just for this special occasion. We can have clipping party and do PAS/Fungus all night long. no, no. Don't thank me, it's my pleasure. ----- Original Message ----- From: "Robyn Vazquez" To: ; ; Sent: Friday, October 19, 2007 4:27 PM Subject: RE: [Histonet] OT: Joe the Toe Party > He will be bringing his ear protectors and toe nail clips...pedicure > anyone?? Happy Friday!!! ;) Robyn > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BMolinari <@t> heart.thi.tmc.edu Mon Oct 22 08:27:49 2007 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Mon Oct 22 08:28:00 2007 Subject: [Histonet] NSH and Halloween/OT world Series In-Reply-To: Message-ID: Count me in for the baseball game tickets. :) -----Original Message----- From: Schneider, Dawn [mailto:SchneiderD@hyhc.com] Sent: Monday, October 22, 2007 7:42 AM To: Molinari, Betsy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NSH and Halloween/OT world Series Yes, someone from our group is trying to get some for Saturday night. Tickets go on sale today. We also are going to the Packer/Bronco game on Monday night and have one extra ticket if anyone is interested. Let me know, Dawn D. Schneider, HT(ASCP) Howard Young Medical Center Woodruff, WI 54568 (715)356-8174 schneiderd@hyhc.com -----Original Message----- From: Molinari, Betsy [mailto:BMolinari@heart.thi.tmc.edu] Sent: Monday, October 22, 2007 6:26 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NSH and Halloween/OT world Series Is anyone interested in trying to get tickets to one of the games? Red Sox fan on the way. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC 1-283 Houston, TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pruegg@ihctech.net Sent: Saturday, October 20, 2007 1:14 PM To: 'Joe Nocito'; 'Bartlett, Jeanine (CDC/CCID/NCZVED)'; 'Kim Merriam'; 'Histonet' Subject: RE: [Histonet] NSH and Halloween If you are coming to Denver in what we call "Rocktober" dressed as a Red Sox or Cleveland Indian, you would be taking your life into your hands. Go Rockies! Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Friday, October 19, 2007 6:20 PM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Kim Merriam; Histonet Subject: Re: [Histonet] NSH and Halloween I'm going to dress up like a Boston Red Sox. Now, that's scary ----- Original Message ----- From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" To: "Kim Merriam" ; "Histonet" Sent: Friday, October 19, 2007 11:49 AM Subject: RE: [Histonet] NSH and Halloween Aren't most of us scary enough already? Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Friday, October 19, 2007 12:47 PM To: Histonet Subject: [Histonet] NSH and Halloween Are any of the vendors having a Halloween party at the NSH? Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic message and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. Dissemination, forwarding, printing or copying of this message/documents without the consent of the sender is prohibited. From carol.lansing <@t> spcorp.com Mon Oct 22 08:32:59 2007 From: carol.lansing <@t> spcorp.com (Lansing, Carol) Date: Mon Oct 22 08:33:14 2007 Subject: [Histonet] NSH and Halloween/OT world Series In-Reply-To: Message-ID: Me too!! :)) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Monday, October 22, 2007 9:28 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NSH and Halloween/OT world Series Count me in for the baseball game tickets. :) -----Original Message----- From: Schneider, Dawn [mailto:SchneiderD@hyhc.com] Sent: Monday, October 22, 2007 7:42 AM To: Molinari, Betsy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NSH and Halloween/OT world Series Yes, someone from our group is trying to get some for Saturday night. Tickets go on sale today. We also are going to the Packer/Bronco game on Monday night and have one extra ticket if anyone is interested. Let me know, Dawn D. Schneider, HT(ASCP) Howard Young Medical Center Woodruff, WI 54568 (715)356-8174 schneiderd@hyhc.com -----Original Message----- From: Molinari, Betsy [mailto:BMolinari@heart.thi.tmc.edu] Sent: Monday, October 22, 2007 6:26 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NSH and Halloween/OT world Series Is anyone interested in trying to get tickets to one of the games? Red Sox fan on the way. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC 1-283 Houston, TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pruegg@ihctech.net Sent: Saturday, October 20, 2007 1:14 PM To: 'Joe Nocito'; 'Bartlett, Jeanine (CDC/CCID/NCZVED)'; 'Kim Merriam'; 'Histonet' Subject: RE: [Histonet] NSH and Halloween If you are coming to Denver in what we call "Rocktober" dressed as a Red Sox or Cleveland Indian, you would be taking your life into your hands. Go Rockies! Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Friday, October 19, 2007 6:20 PM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Kim Merriam; Histonet Subject: Re: [Histonet] NSH and Halloween I'm going to dress up like a Boston Red Sox. Now, that's scary ----- Original Message ----- From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" To: "Kim Merriam" ; "Histonet" Sent: Friday, October 19, 2007 11:49 AM Subject: RE: [Histonet] NSH and Halloween Aren't most of us scary enough already? Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Friday, October 19, 2007 12:47 PM To: Histonet Subject: [Histonet] NSH and Halloween Are any of the vendors having a Halloween party at the NSH? Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic message and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. Dissemination, forwarding, printing or copying of this message/documents without the consent of the sender is prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From ryaskovich <@t> dir.nidcr.nih.gov Mon Oct 22 09:16:04 2007 From: ryaskovich <@t> dir.nidcr.nih.gov (Yaskovich, Ruth A (NIH/NIDCR) [E]) Date: Mon Oct 22 09:16:15 2007 Subject: [Histonet] Video Taping Message-ID: Are any of you (that do patient work) being video taped while at work? This means having a camera on you while you are working in your lab. If so, do you know if it's legal? Is it done some states versus others? I'm posting this for a friend. Thanks for any help you can send our way. Ruth Yaskovich National Institutes of Health Institute of Dental and Crainiofacial Research From rjbuesa <@t> yahoo.com Mon Oct 22 09:25:15 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Oct 22 09:25:20 2007 Subject: [Histonet] Video Taping In-Reply-To: Message-ID: <596502.40789.qm@web61225.mail.yahoo.com> Although I personally would NOT allow it, the opinions you are going to obtain from Histonet are wrthless. Consult with an attorney, any one will give you a "first free consultations", use it on that question. Ren? J. "Yaskovich, Ruth A (NIH/NIDCR) [E]" wrote: Are any of you (that do patient work) being video taped while at work? This means having a camera on you while you are working in your lab. If so, do you know if it's legal? Is it done some states versus others? I'm posting this for a friend. Thanks for any help you can send our way. Ruth Yaskovich National Institutes of Health Institute of Dental and Crainiofacial Research _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From jfish <@t> gladstone.ucsf.edu Mon Oct 22 10:21:16 2007 From: jfish <@t> gladstone.ucsf.edu (Jo Dee Fish) Date: Mon Oct 22 10:22:29 2007 Subject: [Histonet] NSH and Halloween/OT world Series In-Reply-To: Message-ID: <000901c814bf$305d9e90$2e0d010a@JFISH> I have someone trying to get tickets as we speak. But, they are locked out of the website, it is quite frustrating!!! ~~Jo Dee Fish~~ Research Technologist III Gladstone Institute of Cardiovascular Disease Telephone: (415) 734-2567 Fax: (415) 355-0824 E-mail: jfish@gladstone.ucsf.edu Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Monday, October 22, 2007 4:26 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NSH and Halloween/OT world Series Is anyone interested in trying to get tickets to one of the games? Red Sox fan on the way. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC 1-283 Houston, TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pruegg@ihctech.net Sent: Saturday, October 20, 2007 1:14 PM To: 'Joe Nocito'; 'Bartlett, Jeanine (CDC/CCID/NCZVED)'; 'Kim Merriam'; 'Histonet' Subject: RE: [Histonet] NSH and Halloween If you are coming to Denver in what we call "Rocktober" dressed as a Red Sox or Cleveland Indian, you would be taking your life into your hands. Go Rockies! Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Friday, October 19, 2007 6:20 PM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Kim Merriam; Histonet Subject: Re: [Histonet] NSH and Halloween I'm going to dress up like a Boston Red Sox. Now, that's scary ----- Original Message ----- From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" To: "Kim Merriam" ; "Histonet" Sent: Friday, October 19, 2007 11:49 AM Subject: RE: [Histonet] NSH and Halloween Aren't most of us scary enough already? Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Friday, October 19, 2007 12:47 PM To: Histonet Subject: [Histonet] NSH and Halloween Are any of the vendors having a Halloween party at the NSH? Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Mon Oct 22 10:25:33 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Oct 22 10:25:39 2007 Subject: [Histonet] Video Taping Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222EFC2@wahtntex2.waht.swest.nhs.uk> "Are any of you (that do patient work) being video taped while at work? This means having a camera on you while you are working in your lab. If so, do you know if it's legal? Is it done some states versus others? I'm posting this for a friend. Thanks for any help you can send our way." Ruth Depends if you are fully clothed or not . I suspect that it's legal in the UK, maybe even obligatory! Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Biology is the least of what makes someone a mother. --Oprah Winfrey This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From tkngflght <@t> yahoo.com Mon Oct 22 10:28:37 2007 From: tkngflght <@t> yahoo.com (Cheryl R. Kerry) Date: Mon Oct 22 10:28:42 2007 Subject: from experience RE: [Histonet] Video Taping In-Reply-To: <596502.40789.qm@web61225.mail.yahoo.com> Message-ID: <006401c814c0$37e6d450$6701a8c0@CHERYLSLAPTOP> Ruth- I've had to walk through this quagmire before--it can be the only answer if all other controls for safe work environments and patient care/safety have been utilized. It sounds like your friend is approaching this with caution and concern--good. Statues for this kind of issue vary wildly by state. If this is in conjunction with a company or an established lab, they likely have an attorney already representing them on this kind of issue. It's best to do it right if there is a need, and only a legal advisor familiar with the laws of your state and the situation you're trying to remedy can answer accurately and with the rights and best interests of all those involved adequately addressed. It is worth the investment. In anticipation of being flamed--there can be situations where this is the only answer and in doing so you PROTECT those in your lab--it is a very hard decision to come to. I wish her the best. Cheryl Cheryl R. Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab, one great tech at a time. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, October 22, 2007 9:25 AM To: Yaskovich, Ruth A (NIH/NIDCR) [E]; histonet Subject: Re: [Histonet] Video Taping Although I personally would NOT allow it, the opinions you are going to obtain from Histonet are wrthless. Consult with an attorney, any one will give you a "first free consultations", use it on that question. Ren? J. "Yaskovich, Ruth A (NIH/NIDCR) [E]" wrote: Are any of you (that do patient work) being video taped while at work? This means having a camera on you while you are working in your lab. If so, do you know if it's legal? Is it done some states versus others? I'm posting this for a friend. Thanks for any help you can send our way. Ruth Yaskovich National Institutes of Health Institute of Dental and Crainiofacial Research _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Mon Oct 22 10:29:20 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Mon Oct 22 10:29:27 2007 Subject: [Histonet] Video Taping In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBF0222EFC2@wahtntex2.waht.swest.nhs.uk> References: <86ADE4EB583CE64799A9924684A0FBBF0222EFC2@wahtntex2.waht.swest.nhs.uk> Message-ID: I have seen the film it's called "Laboratory Wives". -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo Rogerson Sent: 22 October 2007 16:26 To: Yaskovich, Ruth A (NIH/NIDCR) [E]; histonet Subject: RE: [Histonet] Video Taping "Are any of you (that do patient work) being video taped while at work? This means having a camera on you while you are working in your lab. If so, do you know if it's legal? Is it done some states versus others? I'm posting this for a friend. Thanks for any help you can send our way." Ruth Depends if you are fully clothed or not . I suspect that it's legal in the UK, maybe even obligatory! Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Biology is the least of what makes someone a mother. --Oprah Winfrey This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Mon Oct 22 10:31:13 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Oct 22 10:31:19 2007 Subject: [Histonet] Video Taping Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222EFC4@wahtntex2.waht.swest.nhs.uk> That's the one where they put them in a Laboratory and made them live together for two weeks? Partly naked and with only Newcastle Brown to drink? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Biology is the least of what makes someone a mother. --Oprah Winfrey This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Pat.Bell <@t> UCHSC.edu Mon Oct 22 10:33:16 2007 From: Pat.Bell <@t> UCHSC.edu (Pat.Bell@UCHSC.edu) Date: Mon Oct 22 10:33:24 2007 Subject: [Histonet] NSH and Halloween/OT world Series In-Reply-To: <000901c814bf$305d9e90$2e0d010a@JFISH> Message-ID: <71FCC52823941D49A4BC39B48C6E3428F3BE4A@java.uchsc.edu> You won't be able to purchase tickets until 10:00 Denver time. You have 27 minutes to wait. Good Luck. Pat Bell HT(ASCP) University of Colo. Health Sciences Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jo Dee Fish Sent: Monday, October 22, 2007 9:21 AM To: 'Molinari, Betsy'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NSH and Halloween/OT world Series I have someone trying to get tickets as we speak. But, they are locked out of the website, it is quite frustrating!!! ~~Jo Dee Fish~~ Research Technologist III Gladstone Institute of Cardiovascular Disease Telephone: (415) 734-2567 Fax: (415) 355-0824 E-mail: jfish@gladstone.ucsf.edu Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Monday, October 22, 2007 4:26 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NSH and Halloween/OT world Series Is anyone interested in trying to get tickets to one of the games? Red Sox fan on the way. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC 1-283 Houston, TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pruegg@ihctech.net Sent: Saturday, October 20, 2007 1:14 PM To: 'Joe Nocito'; 'Bartlett, Jeanine (CDC/CCID/NCZVED)'; 'Kim Merriam'; 'Histonet' Subject: RE: [Histonet] NSH and Halloween If you are coming to Denver in what we call "Rocktober" dressed as a Red Sox or Cleveland Indian, you would be taking your life into your hands. Go Rockies! Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Friday, October 19, 2007 6:20 PM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Kim Merriam; Histonet Subject: Re: [Histonet] NSH and Halloween I'm going to dress up like a Boston Red Sox. Now, that's scary ----- Original Message ----- From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" To: "Kim Merriam" ; "Histonet" Sent: Friday, October 19, 2007 11:49 AM Subject: RE: [Histonet] NSH and Halloween Aren't most of us scary enough already? Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Friday, October 19, 2007 12:47 PM To: Histonet Subject: [Histonet] NSH and Halloween Are any of the vendors having a Halloween party at the NSH? Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sheila_adey <@t> hotmail.com Mon Oct 22 10:51:57 2007 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Mon Oct 22 10:52:03 2007 Subject: [Histonet] AEC disposal Message-ID: Hello all, I'm just wondering how other institutions handle their AEC waste from the IHCs? Sheila Adey HT MLTPort Huron HospitalMichigan _________________________________________________________________ Send a smile, make someone laugh, have some fun! Start now! http://www.freemessengeremoticons.ca/?icid=EMENCA122 From ifc1028 <@t> yahoo.com Mon Oct 22 11:01:57 2007 From: ifc1028 <@t> yahoo.com (Irene Cochran) Date: Mon Oct 22 11:08:42 2007 Subject: [Histonet] Histotech Job Opening Message-ID: <714212.44511.qm@web45608.mail.sp1.yahoo.com> Experienced HT (ASCP) certified Tech needed for a full time day position at Bristol Hospital in the Central Connecticut area. Please send resume to mnormand@bristolhospital.org __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From AGrobe2555 <@t> aol.com Mon Oct 22 11:14:00 2007 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Mon Oct 22 11:14:30 2007 Subject: [Histonet] CD3 antibody Message-ID: I have used the Dako anti-CD3 polyclonal on both rabbit and sheep tissue fixed in Histochoice without any antigen retrieval. Stains quite well. I can't give you a dilution as it was 5-6 years back and I don't remember exactly... Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital ************************************** See what's new at http://www.aol.com From rjbuesa <@t> yahoo.com Mon Oct 22 11:15:23 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Oct 22 11:15:29 2007 Subject: [Histonet] AEC disposal In-Reply-To: Message-ID: <945613.87016.qm@web61219.mail.yahoo.com> Sheila: About 20 years ago AEC was considered harmless UNTIL it was discovered it was as cancerigenous ad DAB. I imagine you will have to dispose of it in the same way, although it can be "neutralized" easier. Ren? J. sheila adey wrote: Hello all, I'm just wondering how other institutions handle their AEC waste from the IHCs? Sheila Adey HT MLTPort Huron HospitalMichigan _________________________________________________________________ Send a smile, make someone laugh, have some fun! Start now! http://www.freemessengeremoticons.ca/?icid=EMENCA122_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From plucas <@t> biopath.org Mon Oct 22 11:50:03 2007 From: plucas <@t> biopath.org (Paula Lucas) Date: Mon Oct 22 11:50:08 2007 Subject: [Histonet] Job Opportunity-Pathology-Southern California (Orange County) Message-ID: <20071022165003.85056230C@arkroyal.cnchost.com> Attention: My company is looking for a temporary employee who can work at Fountain Valley Hospital as a secretary to the pathologists for 7 weeks. We are looking for someone who can type, use email, answer phones, use the computer, and be able to work with PowerPoint presentation. The hours are from 8am to 5 pm Monday to Friday. Paula Lucas Bio-Path Medical Group Fountain Valley, CA From CSwanson <@t> biotechnics-inc.com Mon Oct 22 12:11:14 2007 From: CSwanson <@t> biotechnics-inc.com (Cynthia Swanson) Date: Mon Oct 22 12:11:25 2007 Subject: [Histonet] cyclic dextrins? Message-ID: <86F3EC338F6AFB408D0115C55F9150171A55F9@BIO-SERVER.biotechnics-inc.local> I need a protocol to detect cyclic dextrins in formalin fixed tissue (dextrin is an acidic hydrolysis digestion product of starch). I didn't find an appropriate protocol in the archives, any help would be greatly appreciated! Cyndi Cynthia Swanson Biotechnics 310 Millstone Dr., Hillsborough, NC 27278 (919) 245-3114 x105 cswanson@biotechnics-inc.com From BMolinari <@t> heart.thi.tmc.edu Mon Oct 22 12:55:52 2007 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Mon Oct 22 12:55:57 2007 Subject: [Histonet] RE: game In-Reply-To: <0E394B648E5284478A6CCB78E5AFDA2705635222@hpes1.HealthPartners.int> Message-ID: The website is jammed.I have 2 family members working on it to no avail. This will be the only way to get reasonably priced tickets..once the free market scalpers get them the sky is the limit. I checked out one of the on line ticket "vendors" and some tickets are $10,000.00..one seat..one game. Way above the MLB price of $225. -----Original Message----- From: Webb, Dorothy L [mailto:Dorothy.L.Webb@HealthPartners.Com] Sent: Monday, October 22, 2007 12:36 PM To: Molinari, Betsy Subject: game I am a baseball fan, and would cheer for the Red Sox because they are an americal league tem ( I really wanted Cleveland in the series!!) and would love to go to a game if it is not totally out of my cost range!! Any ideas on getting tickets? I tried to get tickets to the Monday Night Football game against the Packers, but they were spendy at 300 plus per ticket! Let me know!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From funderwood <@t> mcohio.org Mon Oct 22 13:06:00 2007 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Mon Oct 22 13:06:20 2007 Subject: [Histonet] RE: game In-Reply-To: References: <0E394B648E5284478A6CCB78E5AFDA2705635222@hpes1.HealthPartners.int> Message-ID: <471CAE48020000340000274C@mcohio.org> This is one of the problems in supporting a winning team. GO REDS :( >>> "Molinari, Betsy" 10/22/2007 1:55 PM >>> The website is jammed.I have 2 family members working on it to no avail. This will be the only way to get reasonably priced tickets..once the free market scalpers get them the sky is the limit. I checked out one of the on line ticket "vendors" and some tickets are $10,000.00..one seat..one game. Way above the MLB price of $225. -----Original Message----- From: Webb, Dorothy L [mailto:Dorothy.L.Webb@HealthPartners.Com] Sent: Monday, October 22, 2007 12:36 PM To: Molinari, Betsy Subject: game I am a baseball fan, and would cheer for the Red Sox because they are an americal league tem ( I really wanted Cleveland in the series!!) and would love to go to a game if it is not totally out of my cost range!! Any ideas on getting tickets? I tried to get tickets to the Monday Night Football game against the Packers, but they were spendy at 300 plus per ticket! Let me know!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Mon Oct 22 13:45:38 2007 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Mon Oct 22 13:42:50 2007 Subject: [Histonet] RE: game In-Reply-To: <471CAE48020000340000274C@mcohio.org> References: <0E394B648E5284478A6CCB78E5AFDA2705635222@hpes1.HealthPartners.int> <471CAE48020000340000274C@mcohio.org> Message-ID: <1F937FB30BDB7C4A9F39F83FEA8D379F3E4CB6@bruexchange1.digestivespecialists.com> Next year for the Reds!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fred Underwood Sent: Monday, October 22, 2007 2:06 PM To: Betsy Molinari; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: game This is one of the problems in supporting a winning team. GO REDS :( >>> "Molinari, Betsy" 10/22/2007 1:55 PM >>> The website is jammed.I have 2 family members working on it to no avail. This will be the only way to get reasonably priced tickets..once the free market scalpers get them the sky is the limit. I checked out one of the on line ticket "vendors" and some tickets are $10,000.00..one seat..one game. Way above the MLB price of $225. -----Original Message----- From: Webb, Dorothy L [mailto:Dorothy.L.Webb@HealthPartners.Com] Sent: Monday, October 22, 2007 12:36 PM To: Molinari, Betsy Subject: game I am a baseball fan, and would cheer for the Red Sox because they are an americal league tem ( I really wanted Cleveland in the series!!) and would love to go to a game if it is not totally out of my cost range!! Any ideas on getting tickets? I tried to get tickets to the Monday Night Football game against the Packers, but they were spendy at 300 plus per ticket! Let me know!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Mon Oct 22 14:14:26 2007 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Mon Oct 22 14:21:11 2007 Subject: [Histonet] Re: fixative recommendation Message-ID: Jacqui Detmar is >>posting this question on behalf of a colleague. She would like to start archiving human placenta tissue and was wondering what would be an ideal fixative.<< and John Kiernan notes - as he and I are always noting - that choosing a fixative with a secret composition isn't a good idea. If a working surgical pathologist from the country with 5% of the world's population and 75% of the world's lawyers may suggest: What we're doing here is archiving tissue for twenty years, since a lawsuit against the obstetrician may be filed until the child reaches majority, and then some. (If your kid flunks out of college, sue the obstetrician.) Certainly paraffin embedding (without cutting sections) would be the safest way to go, and would probably result in the smallest volume of material you had to store. Alternatively you might fix the tissue in neutral buffered formalin (after dissection of the specimen), wash the formaldehyde out, and store the tissue in 70% alcohol. You could then seal the tissue into plastic bags (can't remember the plastic - may have been PVC). I've seen archival autopsy tissue stored like this, and be recoverable after decades. Of course - in the USA anyway - all this work would get you and the pathologist paid only the low fee for a gross examination. Since most of the pathologist's time is spent grossing the specimen rather than doing the microscopy, archiving tissue without preparing slides makes sense only if the pathologist's time is reckoned as being worth nothing. Which of course is usually the case. Bob Richmond Samurai Pathologist (and always reluctant placentologist) Knoxville TN From LSPrestridge <@t> seton.org Mon Oct 22 14:30:16 2007 From: LSPrestridge <@t> seton.org (Prestridge, Linda S.) Date: Mon Oct 22 14:30:21 2007 Subject: [Histonet] plasma cell staining with Ventana Ultraview kit Message-ID: <3D79F47DC92B204F9E5D35C885DFC5CB01433976@AUSEX2VS1.seton.org> While doing CKAE1/AE3's on the XT Benchmark, we are having plasma cell staining and histiocytes staining - especially on our sentinel nodes cases which I now must do on our Dako stainer. Does anyone have a solution to this problem. Evidently the Ultraview detection kit stains plasma cells (confirmed by a Ventana rep.) Does any one have a protocol that stops this staining? Linda From Rcartun <@t> harthosp.org Mon Oct 22 15:01:15 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Mon Oct 22 15:01:27 2007 Subject: [Histonet] plasma cell staining with Ventana Ultraview kit In-Reply-To: <3D79F47DC92B204F9E5D35C885DFC5CB01433976@AUSEX2VS1.seton.org> References: <3D79F47DC92B204F9E5D35C885DFC5CB01433976@AUSEX2VS1.seton.org> Message-ID: <471CC94B0200007700008B1B@gwmail4.harthosp.org> Extra-follicular dendritic cells in lymph nodes will stain with some cytokeratin antibodies. I use this staining as an internal positive control. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Prestridge, Linda S." 10/22/07 3:30 PM >>> While doing CKAE1/AE3's on the XT Benchmark, we are having plasma cell staining and histiocytes staining - especially on our sentinel nodes cases which I now must do on our Dako stainer. Does anyone have a solution to this problem. Evidently the Ultraview detection kit stains plasma cells (confirmed by a Ventana rep.) Does any one have a protocol that stops this staining? Linda _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From barry_m <@t> ozemail.com.au Mon Oct 22 16:40:53 2007 From: barry_m <@t> ozemail.com.au (Barry Madigan) Date: Mon Oct 22 16:41:02 2007 Subject: [Histonet] Menin antibody Message-ID: <000501c814f4$396671d0$ac335570$@com.au> I was wondering if anyone is using the nuclear protein Menin antibody which is suspected to be a tumour suppressor. I have been using the Menin (N19) antibody with little result. Regards Barry B Madigan Immunohistochemistry Pathology Queensland Royal Brisbane Hospital Australia From victor <@t> pathology.washington.edu Mon Oct 22 16:53:33 2007 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Mon Oct 22 16:53:38 2007 Subject: [Histonet] WS Tickets on Craigs List Message-ID: <471D1BDD.7060602@pathology.washington.edu> The tickets appear to be selling in all price ranges. http://denver.craigslist.org/tix/ Good luck Victor -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From lscott <@t> sfcn.org Mon Oct 22 19:22:12 2007 From: lscott <@t> sfcn.org (Scott Hendricksen) Date: Mon Oct 22 19:22:37 2007 Subject: [Histonet] Please remove me from the mailing list. Thanks Message-ID: <006501c8150a$c245f500$0200a8c0@Katsu> Please remove me from the mailing list. Thanks From jnocito <@t> satx.rr.com Mon Oct 22 19:52:49 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Mon Oct 22 20:02:34 2007 Subject: [Histonet] Video Taping References: <86ADE4EB583CE64799A9924684A0FBBF0222EFC2@wahtntex2.waht.swest.nhs.uk> Message-ID: <00fb01c81510$631cf000$0302a8c0@yourxhtr8hvc4p> no, but I know some New England Patriot players ----- Original Message ----- From: "Kemlo Rogerson" To: "Yaskovich, Ruth A (NIH/NIDCR) [E]" ; "histonet" Sent: Monday, October 22, 2007 10:25 AM Subject: RE: [Histonet] Video Taping "Are any of you (that do patient work) being video taped while at work? This means having a camera on you while you are working in your lab. If so, do you know if it's legal? Is it done some states versus others? I'm posting this for a friend. Thanks for any help you can send our way." Ruth Depends if you are fully clothed or not . I suspect that it's legal in the UK, maybe even obligatory! Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Biology is the least of what makes someone a mother. --Oprah Winfrey This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leba <@t> hawaii.edu Tue Oct 23 00:03:49 2007 From: leba <@t> hawaii.edu (Heather A Leba) Date: Tue Oct 23 00:03:59 2007 Subject: [Histonet] Please remove me from the mailing list. Thanks In-Reply-To: <006501c8150a$c245f500$0200a8c0@Katsu> References: <006501c8150a$c245f500$0200a8c0@Katsu> Message-ID: Please remove me from this mailing list. Thank you. From jkiernan <@t> uwo.ca Tue Oct 23 00:59:48 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Tue Oct 23 00:59:54 2007 Subject: [Histonet] cyclic dextrins? Message-ID: What are the "cyclic dextrins" that you are asked to stain in "formalin fixed tissue"? Are they cyclodextrins, dextrins or dextrans? If the fixative was mostly water, dextrins (and dextrans if present) have probably all been dissolved out. These oligo- and polysaccharides are soluble in water. Unlike proteins, they do not react with formaldehyde to form insoluble products. Read on. Cyclodextrins are made by the action of a bacterial amylase on starch. The long chain of 1-->4-linked glucose units is broken down to shorter chains (6, 7 or 8 units), with the ends of each chain joined 1-->4 to make a ring. There are three types of cyclodextrin: alpha (smallest, with 6 glucose units), beta (7), and gamma (8 units). In three dimensions the ring is a shell or cage, with hydrophilic -OH groups of glucose units on the outside and a hydrophobic interior. A hydrophobic organic molecule can be enclosed within a cyclodextrin molecule without chemical change. The complex, known as a clathrate, is soluble in water; this provides a way to make an aqueous solution of an otherwise insoluble hydrophobic substance. The ordinary non-cyclic dextrins (3 types) are produced by either dry heating (toast) or acid hydrolysis of starch. Properties vary with method of production. They are soluble in water and precipitated by addition of alcohol. The information in this and the previous paragraph is from the Merck Index (which gives some references) and from an old biochemistry textbook for medical students (West & Todd, 1956). I do not have any authoritative statement about the solubilities of the three cyclodextrins in liquids other than water. They may, like simple dextrins, be insoluble in alcohols. You can be sure they won't dissolve in xylene or paraffin. Dextrins must not be confused with dextrans, which are bacterial polysaccharides composed of glucose units linked 1-->6 (in contrast to the 1-->4 of plants and animals). Dextrans are the protein substitutes in traditional artificial plasma expanders (Rheomacrodex when I was young; is it still used?) and they can end up in the renal tubules. According to Lillie's big book, which cites a paper by Bob Mowry (Am. J. Path. 29:523, 1953), water must be avoided at all stages prior to staining dextran. That means a non-aqueous fixative and floating out paraffin sections on 95% alcohol, not water. The reagents for staining (periodic acid-Schiff) should be made up in alcohol rather than water. Mowry's and Lillie's simple rules probably apply also to the histochemical detection of cyclo- and other dextrins: Non-aqueous fixative --> wax --> flatten on warm alcohol --> dewax with xylene and then --> alcoholic PAS method. Does this help? Please reply to all. John Kiernan Anatomy, UWO London, Canada --- ----- Original Message ----- From: Cynthia Swanson Date: Monday, October 22, 2007 13:13 Subject: [Histonet] cyclic dextrins? To: histonet@lists.utsouthwestern.edu > I need a protocol to detect cyclic dextrins in formalin fixed tissue > (dextrin is an acidic hydrolysis digestion product of starch). I > didn'tfind an appropriate protocol in the archives, any help > would be greatly > appreciated! > > > > Cyndi > > > > > > Cynthia Swanson > > Biotechnics > > 310 Millstone Dr., > > Hillsborough, NC 27278 > > (919) 245-3114 x105 > > cswanson@biotechnics-inc.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From BMolinari <@t> heart.thi.tmc.edu Tue Oct 23 08:07:50 2007 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Tue Oct 23 08:07:53 2007 Subject: [Histonet] 2nd chance Message-ID: The Rockies had to shut the website down after 2 hrs..it crashed actually..8.5 million hits in 2 hrs. They will go back on line today at 12:00 MT. Betsy Molinari Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-6812 From talulahgosh <@t> gmail.com Tue Oct 23 08:31:31 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Oct 23 08:31:36 2007 Subject: [Histonet] testifying in court? Message-ID: I would like an honest opinion of the histologists, DNA technologists, etc (those names can appear in court trials, or those connected with you)--do you feel nervous offering your opinion in trial to someone's sentence? I ask because this is the ONLY reason I've never applied for forensic science positions---I adore the field, and am possibly beneficial to a PCR/DNA investigation. Emily -- Yog-Sothoth knows the gate. Yog-Sothoth is the gate. Yog-Sothoth is the key and guardian of the gate. Past, present, future, all are one in Yog-Sothoth. He knows where the Old Ones broke through of old, and where They shall break through again. From CSwanson <@t> biotechnics-inc.com Tue Oct 23 08:31:46 2007 From: CSwanson <@t> biotechnics-inc.com (Cynthia Swanson) Date: Tue Oct 23 08:32:01 2007 Subject: [Histonet] cyclic dextrins? In-Reply-To: References: Message-ID: <86F3EC338F6AFB408D0115C55F9150171A563B@BIO-SERVER.biotechnics-inc.local> Thank you for a very informative post. Would you please recommend a non-aqueous fixative? Cyndi ________________________________ What are the "cyclic dextrins" that you are asked to stain in "formalin fixed tissue"? Are they cyclodextrins, dextrins or dextrans? If the fixative was mostly water, dextrins (and dextrans if present) have probably all been dissolved out. These oligo- and polysaccharides are soluble in water. Unlike proteins, they do not react with formaldehyde to form insoluble products. Read on. Cyclodextrins are made by the action of a bacterial amylase on starch. The long chain of 1-->4-linked glucose units is broken down to shorter chains (6, 7 or 8 units), with the ends of each chain joined 1-->4 to make a ring. There are three types of cyclodextrin: alpha (smallest, with 6 glucose units), beta (7), and gamma (8 units). In three dimensions the ring is a shell or cage, with hydrophilic -OH groups of glucose units on the outside and a hydrophobic interior. A hydrophobic organic molecule can be enclosed within a cyclodextrin molecule without chemical change. The complex, known as a clathrate, is soluble in water; this provides a way to make an aqueous solution of an otherwise insoluble hydrophobic substance. The ordinary non-cyclic dextrins (3 types) are produced by either dry heating (toast) or acid hydrolysis of starch. Properties vary with method of production. They are soluble in water and precipitated by addition of alcohol. The information in this and the previous paragraph is from the Merck Index (which gives some references) and from an old biochemistry textbook for medical students (West & Todd, 1956). I do not have any authoritative statement about the solubilities of the three cyclodextrins in liquids other than water. They may, like simple dextrins, be insoluble in alcohols. You can be sure they won't dissolve in xylene or paraffin. Dextrins must not be confused with dextrans, which are bacterial polysaccharides composed of glucose units linked 1-->6 (in contrast to the 1-->4 of plants and animals). Dextrans are the protein substitutes in traditional artificial plasma expanders (Rheomacrodex when I was young; is it still used?) and they can end up in the renal tubules. According to Lillie's big book, which cites a paper by Bob Mowry (Am. J. Path. 29:523, 1953), water must be avoided at all stages prior to staining dextran. That means a non-aqueous fixative and floating out paraffin sections on 95% alcohol, not water. The reagents for staining (periodic acid-Schiff) should be made up in alcohol rather than water. Mowry's and Lillie's simple rules probably apply also to the histochemical detection of cyclo- and other dextrins: Non-aqueous fixative --> wax --> flatten on warm alcohol --> dewax with xylene and then --> alcoholic PAS method. Does this help? Please reply to all. John Kiernan Anatomy, UWO London, Canada --- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From derek.papalegis <@t> tufts.edu Tue Oct 23 08:40:07 2007 From: derek.papalegis <@t> tufts.edu (Derek Papalegis) Date: Tue Oct 23 08:40:15 2007 Subject: [Histonet] lillie-twort Message-ID: <471DF9B7.7070803@tufts.edu> Has anyone used the Lillie Twort kit from Poly scientific? I recently purchased this kit and the results are not acceptable. I was wondering if anyone else has used this and had to tweak it to get the desired results. I noticed that in the procedure that came with the reagents, there is no Safranin step but in every search I did in the histonet archives Safranin is part of their procedure. Any help would be greatly appreciated. Thanks, Derek From kmerriam2003 <@t> yahoo.com Tue Oct 23 09:06:24 2007 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Tue Oct 23 09:06:32 2007 Subject: [Histonet] TNF-alpha antibodies Message-ID: <379300.91827.qm@web50305.mail.re2.yahoo.com> What antibodies are people using for TNF-alpha on human FFPE tissues? Thanks, Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From Stacy_McLaughlin <@t> cooley-dickinson.org Tue Oct 23 10:29:48 2007 From: Stacy_McLaughlin <@t> cooley-dickinson.org (Stacy McLaughlin) Date: Tue Oct 23 10:30:01 2007 Subject: [Histonet] Ventana DAB detections Message-ID: Hi, We are going to switch from the basic DAB kit to either the IVIEW or UltraView. Do any of you have preferences between the two? If so- what are the reasons? Thanks, Stacy McLaughlin Cooley Dickinson Hospital From pex0220 <@t> yahoo.com.cn Tue Oct 23 11:08:52 2007 From: pex0220 <@t> yahoo.com.cn (docqian) Date: Tue Oct 23 11:09:02 2007 Subject: [Histonet] immunohistochemistry method Message-ID: <558260.41746.qm@web15206.mail.cnb.yahoo.com> Dear all,=0A =0AI have one question to ask for your help.=0AI would like to= check one antibody with immunohistochemistry method in paraffin-embedded t= issues and this antibody is a membrane protein, however after this staing, = I find that there are also strong stainings in cytoplasm, I do not know the= reason. Does anyone have some ideas about it? And how can I only label the= membrane?=0A =0AThank you!=0A =0AGuofeng=0A=0A=0A ___________________= ________________________________________ =0A@yahoo.cn =D0=C2=D3=F2=C3=FB=A1= =A2=CE=DE=CF=DE=C1=BF=A3=AC=BF=EC=C0=B4=C7=C0=D7=A2=A3=A1 =0Ahttp://mail.ya= hoo.cn/From LSebree <@t> uwhealth.org Tue Oct 23 11:10:15 2007 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Tue Oct 23 11:10:19 2007 Subject: [Histonet] Ventana DAB detections In-Reply-To: Message-ID: iView is less expensive and has a longer shelf life. The one advantage to the UltraView is that it's a polymer detection system but for the short time we used it we could not use it up before it expired. Due to the higher expense we only used it for cases that had high endogenous biotin. So we are now using the iView DAB kit and using the AB block kit when needed. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stacy McLaughlin Sent: Tuesday, October 23, 2007 10:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana DAB detections Hi, We are going to switch from the basic DAB kit to either the IVIEW or UltraView. Do any of you have preferences between the two? If so- what are the reasons? Thanks, Stacy McLaughlin Cooley Dickinson Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Tue Oct 23 11:17:27 2007 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Tue Oct 23 11:17:46 2007 Subject: [Histonet] RE: game In-Reply-To: References: <0E394B648E5284478A6CCB78E5AFDA2705635222@hpes1.HealthPartners.int> Message-ID: <009701c81590$36a3fd90$0202a8c0@ihctechq9h2qof> Yesterday the website was hacked and not many were able to buy tickets, they are supposed to try again today at noon MT on www.coloradorockies.com I would not trust the tickets on sale thru Craigs list or ebay , this website is supposed to be the only place to buy ws tickets in Denver. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Monday, October 22, 2007 11:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: game The website is jammed.I have 2 family members working on it to no avail. This will be the only way to get reasonably priced tickets..once the free market scalpers get them the sky is the limit. I checked out one of the on line ticket "vendors" and some tickets are $10,000.00..one seat..one game. Way above the MLB price of $225. -----Original Message----- From: Webb, Dorothy L [mailto:Dorothy.L.Webb@HealthPartners.Com] Sent: Monday, October 22, 2007 12:36 PM To: Molinari, Betsy Subject: game I am a baseball fan, and would cheer for the Red Sox because they are an americal league tem ( I really wanted Cleveland in the series!!) and would love to go to a game if it is not totally out of my cost range!! Any ideas on getting tickets? I tried to get tickets to the Monday Night Football game against the Packers, but they were spendy at 300 plus per ticket! Let me know!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Tue Oct 23 11:34:36 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Tue Oct 23 11:35:10 2007 Subject: [Histonet] cyclic dextrins? Message-ID: I can't really recommend a fixative because I have never tried to stain for any type of dextrin in tissues and have never seen a published method for doing so. That doesn't mean there isn't one. In your original post you said the specimens were already fixed in formalin (presumably aqueous); they may well not contain any cyclodextrin. If you must start from scratch, the first place to look is in a library. Mowry's 1953 paper in Am. J. Path. should be worth looking at, even though dextran is not the same as dextrin. Do a cited reference search with Scopus or Web of Science (the best two) or with Google Scholar (also good, and freely available to all). Find publications that cite R.Mowry 1953, and follow up the ones with likely-looking titles. Another valuable resource is www.jhc.org where anyone can search the whole of the Journal of Histochemistry and Cytochemistry (since it began, in the early 1950s) and download (free) the full text of any papers published more than about a year ago. Perhaps you'll find that nobody has wanted to stain cyclodextrin in tissue before. In that case you will have the opportunity to devise a new technique. John Kiernan Anatomy, UWO London, Canada --- ----- Original Message ----- From: Cynthia Swanson Date: Tuesday, October 23, 2007 9:33 Subject: RE: [Histonet] cyclic dextrins? To: John Kiernan Cc: histonet@lists.utsouthwestern.edu > Thank you for a very informative post. Would you please > recommend a > non-aqueous fixative? > > > > Cyndi > > > > ________________________________ > > > > What are the "cyclic dextrins" that you are asked to stain in > "formalinfixed tissue"? Are they cyclodextrins, dextrins or > dextrans? > > > > If the fixative was mostly water, dextrins (and dextrans if present) > have probably all been dissolved out. These oligo- and polysaccharides > are soluble in water. Unlike proteins, they do not react with > formaldehyde to form insoluble products. Read on. > > > > Cyclodextrins are made by the action of a bacterial amylase on starch. > The long chain of 1-->4-linked glucose units is broken > down to shorter > chains (6, 7 or 8 units), with the ends of each chain joined 1-- > >4 to > make a ring. There are three types of cyclodextrin: alpha (smallest, > with 6 glucose units), beta (7), and gamma (8 units). In three > dimensions the ring is a shell or cage, with hydrophilic -OH > groups of > glucose units on the outside and a hydrophobic interior. A hydrophobic > organic molecule can be enclosed within a cyclodextrin molecule > withoutchemical change. The complex, known as a clathrate, is > soluble in water; > this provides a way to make an aqueous solution of an otherwise > insoluble hydrophobic substance. > > > > The ordinary non-cyclic dextrins (3 types) are produced by > either dry > heating (toast) or acid hydrolysis of starch. Properties vary with > method of production. They are soluble in water and precipitated by > addition of alcohol. The information in this and the previous > paragraphis from the Merck Index (which gives some references) > and from an old > biochemistry textbook for medical students (West & Todd, 1956). > > > > I do not have any authoritative statement about the solubilities > of the > three cyclodextrins in liquids other than water. They may, like simple > dextrins, be insoluble in alcohols. You can be sure they won't > dissolve in xylene or paraffin. > > > > Dextrins must not be confused with dextrans, which are bacterial > polysaccharides composed of glucose units linked 1-->6 (in > contrast to > the 1-->4 of plants and animals). Dextrans are the protein substitutes > in traditional artificial plasma expanders (Rheomacrodex when I was > young; is it still used?) and they can end up in the renal tubules. > According to Lillie's big book, which cites a paper by Bob Mowry > (Am. J. > Path. 29:523, 1953), water must be avoided at all stages prior to > staining dextran. That means a non-aqueous fixative and floating out > paraffin sections on 95% alcohol, not water. The reagents for staining > (periodic acid-Schiff) should be made up in alcohol rather than > water. > > > > Mowry's and Lillie's simple rules probably apply also to the > histochemical detection of cyclo- and other dextrins: Non-aqueous > fixative --> wax --> flatten on warm alcohol --> dewax > with xylene and > then --> alcoholic PAS method. Does this help? > Please reply to all. > > > > John Kiernan > > Anatomy, UWO > > London, Canada > --- > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > From rjbuesa <@t> yahoo.com Tue Oct 23 11:37:09 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Oct 23 11:37:13 2007 Subject: [Histonet] immunohistochemistry method In-Reply-To: <558260.41746.qm@web15206.mail.cnb.yahoo.com> Message-ID: <457134.75963.qm@web61211.mail.yahoo.com> Check your titration "checker-board" against a good membrane positive control, and use the highest dilution that stains just the membrane, and use that dilution in another test with the same tissue you are having the problem. Probably you are using too much antibody (more than required).. Ren? J. docqian wrote: Dear all, I have one question to ask for your help. I would like to check one antibody with immunohistochemistry method in paraffin-embedded tissues and this antibody is a membrane protein, however after this staing, I find that there are also strong stainings in cytoplasm, I do not know the reason. Does anyone have some ideas about it? And how can I only label the membrane? Thank you! Guofeng ___________________________________________________________ @yahoo.cn ?????????????????????????? http://mail.yahoo.cn/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From ktuttle <@t> umm.edu Tue Oct 23 11:37:52 2007 From: ktuttle <@t> umm.edu (Kimberly Tuttle) Date: Tue Oct 23 11:38:11 2007 Subject: [Histonet] Stratifin and PSCA In-Reply-To: <558260.41746.qm@web15206.mail.cnb.yahoo.com> References: <558260.41746.qm@web15206.mail.cnb.yahoo.com> Message-ID: <471DEB1F.90CE.001A.3@umm.edu> Can anyone recommend where to buy Stratifin (1433 Sigma) and also PSCA? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From gu.lang <@t> gmx.at Tue Oct 23 12:20:59 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Oct 23 12:21:12 2007 Subject: AW: [Histonet] Ventana DAB detections In-Reply-To: Message-ID: <000001c81599$1544f740$6412a8c0@dielangs.at> The polymer kit has the advantage, that you don't have to think about endogenous biotin. The disadvantage is, that in denser parts of the tissue the polymer "sticks" and gives a higher background. We had no problems with shelflife. When we receive the kit, shelfelife is usually about 3-4 months. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Stacy McLaughlin Gesendet: Dienstag, 23. Oktober 2007 17:30 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Ventana DAB detections Hi, We are going to switch from the basic DAB kit to either the IVIEW or UltraView. Do any of you have preferences between the two? If so- what are the reasons? Thanks, Stacy McLaughlin Cooley Dickinson Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alonso.martinezcanabal <@t> utoronto.ca Tue Oct 23 12:51:42 2007 From: alonso.martinezcanabal <@t> utoronto.ca (alonso.martinezcanabal@utoronto.ca) Date: Tue Oct 23 12:52:23 2007 Subject: [Histonet] Tau-P antibody Message-ID: <20071023135142.wr45g5cruskw8ck0@webmail.utoronto.ca> Dear everyone, Have any of you have ever tried Immunohistochemistry against pTAU antibody in mouse brain. I have seen a lot of commercial antibodies and for my previous experiences I am very careful when I chose an anti phosphor antibody. Any advice or previous experience in this field, would be very useful for me. Thanks a lot Alonso From alonso.martinezcanabal <@t> utoronto.ca Tue Oct 23 12:52:33 2007 From: alonso.martinezcanabal <@t> utoronto.ca (alonso.martinezcanabal@utoronto.ca) Date: Tue Oct 23 12:53:07 2007 Subject: [Histonet] DAB GFP staining Message-ID: <20071023135233.fpbg3msvko0k0kgw@webmail.utoronto.ca> Dear everyone. Have someone ever tried to perform a DAB immunohistochesmitry in tissue transfected with GFP? Some people in my lab have been trying it with not very good results. This is hippocampus and apparently just the nuclei and not the branches are stained. Thanks. Alonso From KMENDELL <@t> nhs-healthlink.org Tue Oct 23 13:44:40 2007 From: KMENDELL <@t> nhs-healthlink.org (Kate Mendell) Date: Tue Oct 23 13:45:01 2007 Subject: [Histonet] Help Tissue falling off Message-ID: <471E08D8020000A80001AB61@mail.nhs-healthlink.org> We have been having a problem with tissue falling off our slides during IHC (benchmark). Nothing has changed we are using the same protocol, same slides, and same control. The controls are staying on and we don't double dip. It doesn't matter if we air dry overnight, microwave or oven dry not all antibodies mostly CD's. Anyone else having problem. We use Colorfrost Plus Micro Slides from Erie Scientfic bought through Cardinal Health. This message and its contents are confidential and are intended for the use of the addressee only, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the intended recipient, this serves as notice that any unauthorized distribution, duplication, printing, or any other use is strictly prohibited. If you feel you have received this email in error, please delete the message and notify the sender so that we may prevent future occurrences. From kbradshaw <@t> lcpath.com Tue Oct 23 13:56:42 2007 From: kbradshaw <@t> lcpath.com (Kari Bradshaw) Date: Tue Oct 23 13:56:48 2007 Subject: [Histonet] Help Tissue falling off In-Reply-To: <471E08D8020000A80001AB61@mail.nhs-healthlink.org> Message-ID: <4ba58e51976fed459c8b3f1e3ceb6119@mail2.lcpath.com> Most recently there has been a problem with a certain lot of detection. If you have another detection kit, I would suggest putting it on. Make sure to call Ventana and let them know if it is indeed the detection kit. Kari Bradshaw Anatomic Pathology Manager Lower Columbia Pathologists 1217 14th Ave Longview, WA 98632 360.425.5620 kbradshaw@lcpath.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kate Mendell Sent: Tuesday, October 23, 2007 11:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Help Tissue falling off We have been having a problem with tissue falling off our slides during IHC (benchmark). Nothing has changed we are using the same protocol, same slides, and same control. The controls are staying on and we don't double dip. It doesn't matter if we air dry overnight, microwave or oven dry not all antibodies mostly CD's. Anyone else having problem. We use Colorfrost Plus Micro Slides from Erie Scientfic bought through Cardinal Health. This message and its contents are confidential and are intended for the use of the addressee only, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the intended recipient, this serves as notice that any unauthorized distribution, duplication, printing, or any other use is strictly prohibited. If you feel you have received this email in error, please delete the message and notify the sender so that we may prevent future occurrences. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Tue Oct 23 14:13:08 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Tue Oct 23 14:13:13 2007 Subject: [Histonet] Knife bevel for methacrylate sectioning? Message-ID: <4EBFF65383B74D49995298C4976D1D5E273CF1@LSRIEXCH1.lsmaster.lifespan.org> What bevel tungsten carbide knives do you use for sectioning large bone blocks? (I am using a Reichert-Jung Polycut E microtome.) How do the results with a 50 degree bevel compare to the results with a 40 degree bevel? Which is better, and why? From mcauliff <@t> umdnj.edu Tue Oct 23 15:21:43 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Oct 23 15:21:58 2007 Subject: [Histonet] alternative IHC marker other than GFAP for astrocytes! In-Reply-To: <6CF686BD6F24A546B85B24FE3B97864701B78F82@EXVS06.net.ucsf.edu> References: <6CF686BD6F24A546B85B24FE3B97864701B78F82@EXVS06.net.ucsf.edu> Message-ID: <471E57D7.1060700@umdnj.edu> S-100 stains astrocytes but is not 100% specific, it does stain other cells. Geoff Mejia, Maria wrote: > Hello, > > I wanting to do fluorescent immunostaining on fixed 40um free-floating primate > sections & I'm looking for a marker other than GFAP! Can anyone recommend > another marker for astrocytes!!!! > > Regards > > Maria Bartola Mejia > Department of Neurosurgery > UCSF > SF, CA > Lab: 415-514-2954 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From dmiller72 <@t> nycap.rr.com Tue Oct 23 16:42:38 2007 From: dmiller72 <@t> nycap.rr.com (DAVID MILLER) Date: Tue Oct 23 16:42:46 2007 Subject: [Histonet] (no subject) Message-ID: <002f01c815bd$a2579470$ba9a4c4a@DAVID> Please remove me from the mailing list thank you David Miller From dmiller72 <@t> nycap.rr.com Tue Oct 23 16:43:40 2007 From: dmiller72 <@t> nycap.rr.com (DAVID MILLER) Date: Tue Oct 23 16:43:47 2007 Subject: [Histonet] REMOVE FROM MAILING LIST Message-ID: <003601c815bd$c6a9fb10$ba9a4c4a@DAVID> PLEASE REMOVE ME FROM THE MAILING LIST THANK YOU DAVID MILLER From shive003 <@t> umn.edu Tue Oct 23 17:24:23 2007 From: shive003 <@t> umn.edu (Jan Shivers) Date: Tue Oct 23 17:24:28 2007 Subject: [Histonet] DAB GFP staining References: <20071023135233.fpbg3msvko0k0kgw@webmail.utoronto.ca> Message-ID: <00b701c815c3$771b8f40$a1065486@auxs.umn.edu> I've done GFP staining with AEC chromogen IHC. If you'd like more info, you can write me directly. Jan Shivers U of Minnesota Veterinary Diagnostic Laboratory shive003@umn.edu ----- Original Message ----- From: To: Sent: Tuesday, October 23, 2007 12:52 PM Subject: [Histonet] DAB GFP staining > Dear everyone. > > Have someone ever tried to perform a DAB immunohistochesmitry > in tissue transfected with GFP? Some people in my lab have been trying it > with not very good results. This is hippocampus and apparently just the > nuclei and not the branches are stained. > > Thanks. > > Alonso > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From koellingr <@t> comcast.net Tue Oct 23 17:36:29 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Tue Oct 23 17:36:34 2007 Subject: [Histonet] DAB GFP staining Message-ID: <102320072236.23171.471E776C000F377500005A8322028887449D09020704040A0105@comcast.net> Alonso, What kind of sections are you using? Are they thick enough? Thin sections would show nuclei and not "branches" since "branches" would randomly be out of the plane of a too thin section. Mainly used flourescent tagging of GFP cells in mouse brain with anti-GFP and a flourescent secondary. But certainly have done this nicely with appropriate secondary and then DAB. Used a goat anti-GFP, (you can use others), a biotinylated horse anti-goat from Vector (I personally like Vector secondaries but can use others), avidin/Peroxidase and DAB. You can actually see a beautiful picture on AbReviews of mouse brain stained with GFP and DAB that I have to admit outdoes anything I did yet they have a very similar procedure although I certainly didn't write the review or produce the slide but mine were pretty darn good. Ray Koelling PhenoPath Labs Seattle, WA -------------- Original message -------------- From: alonso.martinezcanabal@utoronto.ca > Dear everyone. > > Have someone ever tried to perform a DAB > immunohistochesmitry in tissue transfected with GFP? Some people in my > lab have been trying it with not very good results. This is > hippocampus and apparently just the nuclei and not the branches are > stained. > > Thanks. > > Alonso > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Robert.Lott <@t> TriadHospitals.com Tue Oct 23 17:39:24 2007 From: Robert.Lott <@t> TriadHospitals.com (Lott, Robert) Date: Tue Oct 23 17:41:01 2007 Subject: [Histonet] Breast Lumpectomies - MACRO sections Message-ID: <518A08C53ED96D419F498D309E64A36A38A58C@CPRTEVS03.triadhospitals.net> Hi everyone, Really looking forward to Denver in just a couple of days!!! Is "anyone" doing Macro sections on "diagnostic" excisional breast lumpectomies... multiple whole cross sections in Macro cassettes and 2" slides for H&E? If you are currently doing this or have done it in the past, I would like to talk you about fixation, processing, microtomy, etc!!! Robert Robert L. Lott, HTL(ASCP) Manager, Anatomic Pathology Trinity Medical Center / LabFirst 800 Montclair Road Birmingham, AL 35213 205-592-5388 205-592-5646 - fax robert.lott@triadhospitals.com From jnocito <@t> satx.rr.com Tue Oct 23 17:52:53 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Oct 23 17:53:03 2007 Subject: [Histonet] Ventana DAB detections References: Message-ID: <007101c815c7$7282a960$0302a8c0@yourxhtr8hvc4p> I vote for the iView over the Ultraview. With the Ultraview, I had more problems with background staining. ----- Original Message ----- From: "Stacy McLaughlin" To: Sent: Tuesday, October 23, 2007 10:29 AM Subject: [Histonet] Ventana DAB detections Hi, We are going to switch from the basic DAB kit to either the IVIEW or UltraView. Do any of you have preferences between the two? If so- what are the reasons? Thanks, Stacy McLaughlin Cooley Dickinson Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Tue Oct 23 17:57:06 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Oct 23 17:57:10 2007 Subject: [Histonet] testifying in court? References: Message-ID: <008f01c815c8$095b4cc0$0302a8c0@yourxhtr8hvc4p> Emily, I always feel nervous every time I give my opinion. I never had the opportunity to testify in court, so I couldn't give you an answer. I know, I'm a big help. Joe ----- Original Message ----- From: "Emily Sours" To: Sent: Tuesday, October 23, 2007 8:31 AM Subject: [Histonet] testifying in court? >I would like an honest opinion of the histologists, DNA technologists, etc > (those names can appear in court trials, or those connected with you)--do > you feel nervous offering your opinion in trial to someone's sentence? I > ask > because this is the ONLY reason I've never applied for forensic science > positions---I adore the field, and am possibly beneficial to a PCR/DNA > investigation. > > Emily > -- > Yog-Sothoth knows the gate. > Yog-Sothoth is the gate. > Yog-Sothoth is the key and guardian of the gate. Past, present, future, > all > are one in Yog-Sothoth. > He knows where the Old Ones broke through of old, and where They shall > break > through again. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sjchtascp <@t> yahoo.com Tue Oct 23 18:59:25 2007 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Tue Oct 23 18:59:29 2007 Subject: [Histonet] Seeking Employment Message-ID: <132152.26854.qm@web38212.mail.mud.yahoo.com> Looking for HT work in the Madison WI or Rockford, Ill area. Temp, contract, FT or PT. Thanks, Steve __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From hodges420 <@t> msn.com Tue Oct 23 21:39:43 2007 From: hodges420 <@t> msn.com (MARY T HODGES) Date: Tue Oct 23 21:39:49 2007 Subject: [Histonet] Help Tissue falling off In-Reply-To: <471E08D8020000A80001AB61@mail.nhs-healthlink.org> References: <471E08D8020000A80001AB61@mail.nhs-healthlink.org> Message-ID: i have heard some of the labs complaining... check your batch of slides... slides were the # 1 reason at Ventana for tissue falling off. sometimes it just a bad batch Tere Hodges> Date: Tue, 23 Oct 2007 14:44:40 -0400> From: KMENDELL@nhs-healthlink.org> To: histonet@lists.utsouthwestern.edu> Subject: [Histonet] Help Tissue falling off> > We have been having a problem with tissue falling off our slides during IHC (benchmark). Nothing has changed we are using the same protocol, same slides, and same control. The controls are staying on and we don't double dip. It doesn't matter if we air dry overnight, microwave or oven dry not all antibodies mostly CD's. Anyone else having problem. We use Colorfrost Plus Micro Slides from Erie Scientfic bought through Cardinal Health.> > This message and its contents are confidential and are intended for the use of the addressee only, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the intended recipient, this serves as notice that any unauthorized distribution, duplication, printing, or any other use is strictly prohibited. If you feel you have received this email in error, please delete the message and notify the sender so that we may prevent future occurrences. > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Boo!?Scare away worms, viruses and so much more! Try Windows Live OneCare! http://onecare.live.com/standard/en-us/purchase/trial.aspx?s_cid=wl_hotmailnews From mtarango <@t> nvcancer.org Tue Oct 23 22:39:35 2007 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Tue Oct 23 22:39:47 2007 Subject: [Histonet] Help Tissue falling off In-Reply-To: <471E08D8020000A80001AB61@mail.nhs-healthlink.org> Message-ID: <5AEC610C1CE02945BD63A395BA763EDE011B6F01@NVCIEXCH02.NVCI.org> Has your fixation or processing times been altered? The fact that the control section adheres and the patient tissue does not, makes me think that you might have a problem with fixation (since you even tried drying overnight). You might want to check it out. Mark Adam Tarango HT(ASCP) Histology & IHC Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 Direct Line (702) 822-5112 Treo (702) 759-9229 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kate Mendell Sent: Tuesday, October 23, 2007 11:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Help Tissue falling off We have been having a problem with tissue falling off our slides during IHC (benchmark). Nothing has changed we are using the same protocol, same slides, and same control. The controls are staying on and we don't double dip. It doesn't matter if we air dry overnight, microwave or oven dry not all antibodies mostly CD's. Anyone else having problem. We use Colorfrost Plus Micro Slides from Erie Scientfic bought through Cardinal Health. This message and its contents are confidential and are intended for the use of the addressee only, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the intended recipient, this serves as notice that any unauthorized distribution, duplication, printing, or any other use is strictly prohibited. If you feel you have received this email in error, please delete the message and notify the sender so that we may prevent future occurrences. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From melissa.mazan <@t> tufts.edu Wed Oct 24 06:26:01 2007 From: melissa.mazan <@t> tufts.edu (Melissa Mazan) Date: Wed Oct 24 06:26:11 2007 Subject: [Histonet] ERK and nuclear counterstain In-Reply-To: <200710221623.l9MGN3Lo001003@mail-proofpoint-3a.usg.tufts.edu> References: <200710221623.l9MGN3Lo001003@mail-proofpoint-3a.usg.tufts.edu> Message-ID: <471F2BC9.1020604@tufts.edu> Hi all, I am doing phospho ERK staining - using the Vector MOM kit on lung tissue - I want to counterstain so that I can enumerate number of ERK pos nuclei with respect to other nuclei, but if I use hematoxylin it obscures the signal - everything just looks dark. Any suggestions for a nuclear counterstain? Many thanks - Melissa histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Tris buffer (Amos Brooks) > 2. Fw: test (Massimo Tosi) > 3. Histotech - open position (Patricia Valente) > 4. Histotech - open position (Patricia Valente) > 5. Re: PAS for frozens (John Kiernan) > 6. Re: fixative recommendation (John Kiernan) > 7. HELP!!! peroxidase blocking on HCC paraffin sections!!!! > (Victor Wong) > 8. Re: tamm-horsfall protein (Andi Kappeler) > 9. RE: NSH and Halloween/OT world Series (Molinari, Betsy) > 10. Re: HELP!!! peroxidase blocking on HCC paraffin sections!!!! > (Rene J Buesa) > 11. RE: OT: Joe the Toe Party (Bernice Frederick) > 12. RE: OT: Joe the Toe Party (Edwards, R.E.) > 13. RE: NSH and Halloween/OT world Series (Molinari, Betsy) > 14. RE: NSH and Halloween/OT world Series (Lansing, Carol) > 15. Video Taping (Yaskovich, Ruth A (NIH/NIDCR) [E]) > 16. Re: Video Taping (Rene J Buesa) > 17. RE: NSH and Halloween/OT world Series (Jo Dee Fish) > 18. RE: Video Taping (Kemlo Rogerson) > 19. from experience RE: [Histonet] Video Taping (Cheryl R. Kerry) > 20. RE: Video Taping (Edwards, R.E.) > 21. RE: Video Taping (Kemlo Rogerson) > 22. RE: NSH and Halloween/OT world Series (Pat.Bell@UCHSC.edu) > 23. AEC disposal (sheila adey) > 24. Histotech Job Opening (Irene Cochran) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sun, 21 Oct 2007 14:02:12 -0400 > From: "Amos Brooks" > Subject: [Histonet] Tris buffer > To: sheila_adey@hotmail.com, histonet@lists.utsouthwestern.edu > Message-ID: > <582736990710211102w460aa022ja88f520ba4deccf1@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > Sheila, > The idea is to make sure the environment the sections are in before > adding the antibodies is as similar to the environment the antibodies > themselves are in as possible. This increases the avidity and affinity of > the antibodies for the antigens. > Think of it like a lock and key. If your hand is shaking you won't be > able to get the key into the lock as well. The molecules of the proteins are > doing the same thing when they are of a different pH (or temperature for > that matter). When the molecules aren't moving around or twisting (like what > happens in antigen retrieval) they can line up and the reactive parts can > get close enough to bond properly. > > Amos Brooks > > > ------------------------------ > > Message: 2 > Date: Mon, 22 Oct 2007 01:24:12 +0200 > From: "Massimo Tosi" > Subject: [Histonet] Fw: test > To: "histonet" > Message-ID: <000701c81439$7d56c8b0$0c01a8c0@SN300208440005> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=response > > > ----- Original Message ----- > From: "Massimo Tosi" > To: "histonet" > Sent: Monday, October 22, 2007 12:56 AM > Subject: test > > > >> test di prova >> > > > > > ------------------------------ > > Message: 3 > Date: Sun, 21 Oct 2007 17:15:40 -0700 (PDT) > From: Patricia Valente > Subject: [Histonet] Histotech - open position > To: histonet@lists.utsouthwestern.edu, > histonet@lists.utsouthwestern.edu > Cc: Brenda Bailey > Message-ID: <53798.312.qm@web81701.mail.mud.yahoo.com> > Content-Type: text/plain; charset=us-ascii > > We have a vacant position for an experienced Histotech > - ASCP and 5 yrs minimum experience preferred. > > Come join our Urological Pathology team in San Antonio. > > For more info - E-mail bbailey@uropathllc.com > or fax resume to: 210 - 521 - 7710 > > > > > > > > > > > ------------------------------ > > Message: 4 > Date: Sun, 21 Oct 2007 17:15:40 -0700 (PDT) > From: Patricia Valente > Subject: [Histonet] Histotech - open position > To: histonet@lists.utsouthwestern.edu, > histonet@lists.utsouthwestern.edu > Cc: Brenda Bailey > Message-ID: <53798.312.qm@web81701.mail.mud.yahoo.com> > Content-Type: text/plain; charset=us-ascii > > We have a vacant position for an experienced Histotech > - ASCP and 5 yrs minimum experience preferred. > > Come join our Urological Pathology team in San Antonio. > > For more info - E-mail bbailey@uropathllc.com > or fax resume to: 210 - 521 - 7710 > > > > > > > > > > > ------------------------------ > > Message: 5 > Date: Mon, 22 Oct 2007 00:18:59 -0400 > From: John Kiernan > Subject: Re: [Histonet] PAS for frozens > To: Mark Elliott > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=us-ascii > > Don't do anything without knowing the reason! Of the four liquids you list, the first three will extract most of the lipids (PAS +ve and others) from the sections. Formalin will not do this (unless it's diluted with a large excess of an organic solvent such as alcohol). You didn't say if the pieces of lung had been fixed before freezing. If not, brief treatment with formalin won't fix them, though it will probably reduce autolysis and delay putrefaction. The three lipid solvents that you list will all rapidly provide good fixation at the micro-anatomical level (e.g. X40 dry objective), as long as you're not interested in lipids or enzyme activity histochemistry. > > John Kiernan > Anatomy, UWO > London, Canada > --- > ----- Original Message ----- > From: Mark Elliott > Date: Sunday, October 21, 2007 22:09 > Subject: Re: [Histonet] PAS for frozens > To: John Kiernan > > >> Thanks very much for the info, it is much appreciated. >> Have received a number of methods with various pre-fixes-none, >> acetic ethanol, ethanol, Carnoys and formalin. Will try a >> variety. >> Mark >> >> >>>>> John Kiernan 10/18/2007 8:22 AM >>> >>>>> >> If your frozen sections are mounted on slides, the periodic acid- >> Schiff PAS procedure is the same as for paraffin sections. >> Lipids, especially hydrophilic ones, are PAS-positive to a >> variable extent. This is partly on account of the sugars in >> glycolipids and partly due to atmospheric oxidation at >> unsaturated bonds. >> >> ----- Original Message ----- >> From: Mark Elliott >> Date: Tuesday, October 16, 2007 13:58 >> Subject: [Histonet] PAS for frozens >> To: histonet@lists.utsouthwestern.edu >> >> >>> Does anyone have a procedure for doing a PAS on frozen >>> tissue?? I assume we need to shorten the times in the >>> various regents, but by how much? Any tips/tricks would >>> >> be >> >>> greatly appreciated. We are staining human lung tissue. >>> >>> Thanks >>> Mark in dreary Vancouver BC >>> > > > ------------------------------ > > Message: 6 > Date: Mon, 22 Oct 2007 00:39:23 -0400 > From: John Kiernan > Subject: Re: [Histonet] fixative recommendation > To: Jacqui Detmar > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=us-ascii > > The effects of a fixative on a tissue depend on the physical properties and chemical reactivities of the ingredients. The requirements of the investigation must also be considered. What will be done with the archived placental tissue? Every textbook of histotechnology, microtechnique or histochemistry explains how to go about choosing a fixative. > > Before buying any proprietary fixative mixture with a trade-name, find out the names and concentrations of all the ingredients. Without this information it is impossible to decide whether the mixture will be suitable for your purposes. If you look up the Histonet archives (www.histosearch.com) you will find many warnings of this kind, from several different people. There is no shortage of excellent fixatives of known composition that you can either mix for yourself or buy ready-made. > > John Kiernan > Anatomy, UWO > London, Canada > --- > ----- Original Message ----- > From: Jacqui Detmar > Date: Saturday, October 20, 2007 13:35 > Subject: [Histonet] fixative recommendation > To: histonet@lists.utsouthwestern.edu > > >> Hi all. I am posting this question on behalf of a >> colleague. She would like to start archiving human >> placenta tissue and was wondering what would be an ideal >> fixative. Any help would be appreciated. >> >> Also, she asked me for my opinion on a product called "HOPE" >> fixative, which is produced by Polysciences, but I have not used >> this fixative. I was hoping someone out there in histoland >> has experience with this fixative and would give me his/her >> opinion. >> >> Lastly, if anybody has any helpful suggestions or advice to give >> re: setting up archives of tissue, please pass this on. >> Fore-warned is fore-armed, eh? >> >> Thanks in advance, >> >> >> Jacqui Detmar, Post-doctoral Fellow >> Samuel Lunenfeld Research Institute, room 876 >> Mount Sinai Hospital >> 600 University Avenue, >> Toronto, ON, Canada >> M5G 1X5 >> >> Tel: 416-586-4800 x2451/x2290 >> Fax: 416-586-8588 >> email: detmar@mshri.on.ca >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > > > ------------------------------ > > Message: 7 > Date: Mon, 22 Oct 2007 01:04:24 -0700 (PDT) > From: Victor Wong > Subject: [Histonet] HELP!!! peroxidase blocking on HCC paraffin > sections!!!! > To: histonet@lists.utsouthwestern.edu > Message-ID: <47703.20903.qm@web52106.mail.re2.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hi all, > > Last time I tried to block endogenous peroxidase with 0.3% hydrogen peroxide for 15 minutes but it came out a lot of background. Then I tried to block with 3% in PBS for 10 and 30 minutes. The background staining was greatly reduced esp. in that treated for 30 minutes. But the positive staining was also muchly reduced. Since I have no spared slides left, I cannot make others trial to massage the blocking conditions. I felt very frustrated and now I am seeking help from you folks. I use the DAKO's Envision+ kit therefore it should not be the endogenous biotin's problem. I also want to know the rationale behind the H2O2 on blocking the peroxide and the visualization of chromogen. Tons of thanks. > > Victor > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > > ------------------------------ > > Message: 8 > Date: Mon, 22 Oct 2007 12:26:51 +0200 > From: "Andi Kappeler" > Subject: Re: [Histonet] tamm-horsfall protein > To: "Bobrowitz, Carol" , "Histonet" > > Message-ID: <00c101c81496$100aeae0$27955c82@patho.unibe.ch> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > Hi Carol > > we use mo-a-THP, clone 10.32, from Cedarlane > (http://www.cedarlanelabs.com/DataSheets/CL1032A.PDF). Working dilution > 11:200, no pretreatment. Hope this helps. > > Best regards > Andi Kappeler > Institute of Pathology, University of Bern, Switzerland > > ----- Original Message ----- > From: "Bobrowitz, Carol" > To: > Sent: Friday, October 19, 2007 4:55 PM > Subject: [Histonet] tamm-horsfall protein > > > Hi, > > > > I need to locate a source for the antibody tamm-horsfall protein. > > > > Does anyone have any experience with this antibody? > > > > Any help will be appreciated. > > > > Thank you in advance. > > > > Carol Ann Bobrowitz > > Medical College of Wisconsin > > Physiology - Histology > > cbobrowi@mcw.edu > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 9 > Date: Mon, 22 Oct 2007 06:25:32 -0500 > From: "Molinari, Betsy" > Subject: RE: [Histonet] NSH and Halloween/OT world Series > To: > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > Is anyone interested in trying to get tickets to one of the games? Red > Sox fan on the way. > > > Betsy Molinari HT (ASCP) > Texas Heart Institute > Cardiovascular Pathology > 6770 Bertner Ave > MC 1-283 > Houston, TX 77030 > 832-355-6524 > 832-355-6812 (fax) > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > pruegg@ihctech.net > Sent: Saturday, October 20, 2007 1:14 PM > To: 'Joe Nocito'; 'Bartlett, Jeanine (CDC/CCID/NCZVED)'; 'Kim Merriam'; > 'Histonet' > Subject: RE: [Histonet] NSH and Halloween > > If you are coming to Denver in what we call "Rocktober" dressed as a Red > Sox > or Cleveland Indian, you would be taking your life into your hands. Go > Rockies! > Patsy > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe > Nocito > Sent: Friday, October 19, 2007 6:20 PM > To: Bartlett, Jeanine (CDC/CCID/NCZVED); Kim Merriam; Histonet > Subject: Re: [Histonet] NSH and Halloween > > I'm going to dress up like a Boston Red Sox. Now, that's scary > ----- Original Message ----- > From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" > To: "Kim Merriam" ; "Histonet" > > Sent: Friday, October 19, 2007 11:49 AM > Subject: RE: [Histonet] NSH and Halloween > > > Aren't most of us scary enough already? > > > Jeanine Bartlett > Infectious Diseases Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim > Merriam > Sent: Friday, October 19, 2007 12:47 PM > To: Histonet > Subject: [Histonet] NSH and Halloween > > Are any of the vendors having a Halloween party at the NSH? > > Kim > > Kim Merriam, MA, HT(ASCP) > Cambridge, MA > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 10 > Date: Mon, 22 Oct 2007 05:53:19 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] HELP!!! peroxidase blocking on HCC paraffin > sections!!!! > To: Victor Wong , histonet@lists.utsouthwestern.edu > Message-ID: <268371.5607.qm@web61225.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Victor: You should be able to quench endogenous peroxidase with 3% UNLESS the H2O2 you are using has "expired" meaning that is NOT really a 3% solution. > My first reccommendation would be to get really fresh H2O2 and try, again, Just 5 minutes at 3%, and that ought to be enough. > The rationale is that peroxidase is an omnipresent enzime in all cells and if not oxidized (with H2O2) will produce a "background noise" (=unspecific staining) during the IHC procedure. > Similarly DAB to react needs the addition of H2O2. > Try a new fresh batch of hydrogen peroxide. > Ren? J. > > Victor Wong wrote: > Hi all, > > Last time I tried to block endogenous peroxidase with 0.3% hydrogen peroxide for 15 minutes but it came out a lot of background. Then I tried to block with 3% in PBS for 10 and 30 minutes. The background staining was greatly reduced esp. in that treated for 30 minutes. But the positive staining was also muchly reduced. Since I have no spared slides left, I cannot make others trial to massage the blocking conditions. I felt very frustrated and now I am seeking help from you folks. I use the DAKO's Envision+ kit therefore it should not be the endogenous biotin's problem. I also want to know the rationale behind the H2O2 on blocking the peroxide and the visualization of chromogen. Tons of thanks. > > Victor > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > > ------------------------------ > > Message: 11 > Date: Mon, 22 Oct 2007 08:12:32 -0500 > From: "Bernice Frederick" > Subject: RE: [Histonet] OT: Joe the Toe Party > To: "'Joe Nocito'" , "'Robyn Vazquez'" > , , > > Message-ID: <000501c814ad$37d45860$d00f7ca5@lurie.northwestern.edu> > Content-Type: text/plain; charset="us-ascii" > > Are you also bringing the Nair to soften those toenails? > > Bernice Frederick HTL (ASCP) > Northwestern University > Pathology Core Facility > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > > > -----Original Message----- > From: Joe Nocito [mailto:jnocito@satx.rr.com] > Sent: Friday, October 19, 2007 6:28 PM > To: Robyn Vazquez; histonet@lists.utsouthwestern.edu; > sbreeden@nmda.nmsu.edu; b-frederick@northwestern.edu > Subject: Re: [Histonet] OT: Joe the Toe Party > > I all want you to know that I haven't clip my toenails in months just for > this special occasion. We can have clipping party and do PAS/Fungus all > night long. no, no. Don't thank me, it's my pleasure. > > ----- Original Message ----- > From: "Robyn Vazquez" > To: ; ; > > Sent: Friday, October 19, 2007 4:27 PM > Subject: RE: [Histonet] OT: Joe the Toe Party > > > >> He will be bringing his ear protectors and toe nail clips...pedicure >> anyone?? Happy Friday!!! ;) >> Robyn >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > > > ------------------------------ > > Message: 12 > Date: Mon, 22 Oct 2007 14:24:31 +0100 > From: "Edwards, R.E." > Subject: RE: [Histonet] OT: Joe the Toe Party > To: "Bernice Frederick" , "Joe Nocito" > , "Robyn Vazquez" , > , > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Perhaps, more likely to be the good old Jim Bean to soften the > senses. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice > Frederick > Sent: 22 October 2007 14:13 > To: 'Joe Nocito'; 'Robyn Vazquez'; histonet@lists.utsouthwestern.edu; > sbreeden@nmda.nmsu.edu > Subject: RE: [Histonet] OT: Joe the Toe Party > > Are you also bringing the Nair to soften those toenails? > > Bernice Frederick HTL (ASCP) > Northwestern University > Pathology Core Facility > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > > > -----Original Message----- > From: Joe Nocito [mailto:jnocito@satx.rr.com] > Sent: Friday, October 19, 2007 6:28 PM > To: Robyn Vazquez; histonet@lists.utsouthwestern.edu; > sbreeden@nmda.nmsu.edu; b-frederick@northwestern.edu > Subject: Re: [Histonet] OT: Joe the Toe Party > > I all want you to know that I haven't clip my toenails in months just > for this special occasion. We can have clipping party and do PAS/Fungus > all night long. no, no. Don't thank me, it's my pleasure. > > ----- Original Message ----- > From: "Robyn Vazquez" > To: ; ; > > Sent: Friday, October 19, 2007 4:27 PM > Subject: RE: [Histonet] OT: Joe the Toe Party > > > >> He will be bringing his ear protectors and toe nail clips...pedicure >> anyone?? Happy Friday!!! ;) Robyn >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 13 > Date: Mon, 22 Oct 2007 08:27:49 -0500 > From: "Molinari, Betsy" > Subject: RE: [Histonet] NSH and Halloween/OT world Series > To: > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > Count me in for the baseball game tickets. :) > > -----Original Message----- > From: Schneider, Dawn [mailto:SchneiderD@hyhc.com] > Sent: Monday, October 22, 2007 7:42 AM > To: Molinari, Betsy; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] NSH and Halloween/OT world Series > > Yes, someone from our group is trying to get some for Saturday night. > Tickets go on sale today. > We also are going to the Packer/Bronco game on Monday night and have one > extra ticket if anyone is interested. > > Let me know, > > Dawn D. Schneider, HT(ASCP) > Howard Young Medical Center > Woodruff, WI 54568 > (715)356-8174 > schneiderd@hyhc.com > > > -----Original Message----- > From: Molinari, Betsy [mailto:BMolinari@heart.thi.tmc.edu] > Sent: Monday, October 22, 2007 6:26 AM > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] NSH and Halloween/OT world Series > > Is anyone interested in trying to get tickets to one of the games? Red > Sox > fan on the way. > > > Betsy Molinari HT (ASCP) > Texas Heart Institute > Cardiovascular Pathology > 6770 Bertner Ave > MC 1-283 > Houston, TX 77030 > 832-355-6524 > 832-355-6812 (fax) > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > pruegg@ihctech.net > Sent: Saturday, October 20, 2007 1:14 PM > To: 'Joe Nocito'; 'Bartlett, Jeanine (CDC/CCID/NCZVED)'; 'Kim Merriam'; > 'Histonet' > Subject: RE: [Histonet] NSH and Halloween > > If you are coming to Denver in what we call "Rocktober" dressed as a Red > Sox > or Cleveland Indian, you would be taking your life into your hands. Go > Rockies! > Patsy > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe > Nocito > Sent: Friday, October 19, 2007 6:20 PM > To: Bartlett, Jeanine (CDC/CCID/NCZVED); Kim Merriam; Histonet > Subject: Re: [Histonet] NSH and Halloween > > I'm going to dress up like a Boston Red Sox. Now, that's scary > ----- Original Message ----- > From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" > To: "Kim Merriam" ; "Histonet" > > Sent: Friday, October 19, 2007 11:49 AM > Subject: RE: [Histonet] NSH and Halloween > > > Aren't most of us scary enough already? > > > Jeanine Bartlett > Infectious Diseases Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim > Merriam > Sent: Friday, October 19, 2007 12:47 PM > To: Histonet > Subject: [Histonet] NSH and Halloween > > Are any of the vendors having a Halloween party at the NSH? > > Kim > > Kim Merriam, MA, HT(ASCP) > Cambridge, MA > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > This electronic message and any files transmitted with it are > confidential and are intended solely for the use of the individual or > entity to whom they are addressed. Dissemination, forwarding, printing > or copying of this message/documents without the consent of the sender > is prohibited. > > > > ------------------------------ > > Message: 14 > Date: Mon, 22 Oct 2007 09:32:59 -0400 > From: "Lansing, Carol" > Subject: RE: [Histonet] NSH and Halloween/OT world Series > To: "Molinari, Betsy" , > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Me too!! :)) > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Molinari, Betsy > Sent: Monday, October 22, 2007 9:28 AM > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] NSH and Halloween/OT world Series > > > Count me in for the baseball game tickets. :) > > -----Original Message----- > From: Schneider, Dawn [mailto:SchneiderD@hyhc.com] > Sent: Monday, October 22, 2007 7:42 AM > To: Molinari, Betsy; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] NSH and Halloween/OT world Series > > Yes, someone from our group is trying to get some for Saturday night. > Tickets go on sale today. We also are going to the Packer/Bronco game on > Monday night and have one extra ticket if anyone is interested. > > Let me know, > > Dawn D. Schneider, HT(ASCP) > Howard Young Medical Center > Woodruff, WI 54568 > (715)356-8174 > schneiderd@hyhc.com > > > -----Original Message----- > From: Molinari, Betsy [mailto:BMolinari@heart.thi.tmc.edu] > Sent: Monday, October 22, 2007 6:26 AM > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] NSH and Halloween/OT world Series > > Is anyone interested in trying to get tickets to one of the games? Red > Sox fan on the way. > > > Betsy Molinari HT (ASCP) > Texas Heart Institute > Cardiovascular Pathology > 6770 Bertner Ave > MC 1-283 > Houston, TX 77030 > 832-355-6524 > 832-355-6812 (fax) > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > pruegg@ihctech.net > Sent: Saturday, October 20, 2007 1:14 PM > To: 'Joe Nocito'; 'Bartlett, Jeanine (CDC/CCID/NCZVED)'; 'Kim Merriam'; > 'Histonet' > Subject: RE: [Histonet] NSH and Halloween > > If you are coming to Denver in what we call "Rocktober" dressed as a Red > Sox or Cleveland Indian, you would be taking your life into your hands. > Go Rockies! Patsy > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe > Nocito > Sent: Friday, October 19, 2007 6:20 PM > To: Bartlett, Jeanine (CDC/CCID/NCZVED); Kim Merriam; Histonet > Subject: Re: [Histonet] NSH and Halloween > > I'm going to dress up like a Boston Red Sox. Now, that's scary > ----- Original Message ----- > From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" > To: "Kim Merriam" ; "Histonet" > > Sent: Friday, October 19, 2007 11:49 AM > Subject: RE: [Histonet] NSH and Halloween > > > Aren't most of us scary enough already? > > > Jeanine Bartlett > Infectious Diseases Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim > Merriam > Sent: Friday, October 19, 2007 12:47 PM > To: Histonet > Subject: [Histonet] NSH and Halloween > > Are any of the vendors having a Halloween party at the NSH? > > Kim > > Kim Merriam, MA, HT(ASCP) > Cambridge, MA > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > This electronic message and any files transmitted with it are > confidential and are intended solely for the use of the individual or > entity to whom they are addressed. Dissemination, forwarding, printing > or copying of this message/documents without the consent of the sender > is prohibited. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ********************************************************************* > This message and any attachments are solely for the > intended recipient. If you are not the intended recipient, > disclosure, copying, use or distribution of the information > included in this message is prohibited -- Please > immediately and permanently delete. > > > > ------------------------------ > > Message: 15 > Date: Mon, 22 Oct 2007 10:16:04 -0400 > From: "Yaskovich, Ruth A (NIH/NIDCR) [E]" > > Subject: [Histonet] Video Taping > To: "histonet" > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Are any of you (that do patient work) being video taped while at work? > This means having a camera on you while you are working in your lab. If > so, do you know if it's legal? Is it done some states versus others? I'm > posting this for a friend. > > Thanks for any help you can send our way. > > Ruth Yaskovich > > National Institutes of Health > > Institute of Dental and Crainiofacial Research > > > > ------------------------------ > > Message: 16 > Date: Mon, 22 Oct 2007 07:25:15 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Video Taping > To: "Yaskovich, Ruth A \(NIH/NIDCR\) \[E\]" > , histonet > > Message-ID: <596502.40789.qm@web61225.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Although I personally would NOT allow it, the opinions you are going to obtain from Histonet are wrthless. > Consult with an attorney, any one will give you a "first free consultations", use it on that question. > Ren? J. > > "Yaskovich, Ruth A (NIH/NIDCR) [E]" wrote: > Are any of you (that do patient work) being video taped while at work? > This means having a camera on you while you are working in your lab. If > so, do you know if it's legal? Is it done some states versus others? I'm > posting this for a friend. > > Thanks for any help you can send our way. > > Ruth Yaskovich > > National Institutes of Health > > Institute of Dental and Crainiofacial Research > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > > ------------------------------ > > Message: 17 > Date: Mon, 22 Oct 2007 08:21:16 -0700 > From: "Jo Dee Fish" > Subject: RE: [Histonet] NSH and Halloween/OT world Series > To: "'Molinari, Betsy'" , > > Message-ID: <000901c814bf$305d9e90$2e0d010a@JFISH> > Content-Type: text/plain; charset="us-ascii" > > I have someone trying to get tickets as we speak. But, they are locked out > of the website, it is quite frustrating!!! > > > ~~Jo Dee Fish~~ > Research Technologist III > Gladstone Institute of Cardiovascular Disease > > Telephone: (415) 734-2567 > Fax: (415) 355-0824 > E-mail: jfish@gladstone.ucsf.edu > > Mailing address: > The J. David Gladstone Institutes > 1650 Owens Street > San Francisco, CA 94158 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, > Betsy > Sent: Monday, October 22, 2007 4:26 AM > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] NSH and Halloween/OT world Series > > Is anyone interested in trying to get tickets to one of the games? Red Sox > fan on the way. > > > Betsy Molinari HT (ASCP) > Texas Heart Institute > Cardiovascular Pathology > 6770 Bertner Ave > MC 1-283 > Houston, TX 77030 > 832-355-6524 > 832-355-6812 (fax) > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > pruegg@ihctech.net > Sent: Saturday, October 20, 2007 1:14 PM > To: 'Joe Nocito'; 'Bartlett, Jeanine (CDC/CCID/NCZVED)'; 'Kim Merriam'; > 'Histonet' > Subject: RE: [Histonet] NSH and Halloween > > If you are coming to Denver in what we call "Rocktober" dressed as a Red > Sox > or Cleveland Indian, you would be taking your life into your hands. Go > Rockies! > Patsy > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe > Nocito > Sent: Friday, October 19, 2007 6:20 PM > To: Bartlett, Jeanine (CDC/CCID/NCZVED); Kim Merriam; Histonet > Subject: Re: [Histonet] NSH and Halloween > > I'm going to dress up like a Boston Red Sox. Now, that's scary > ----- Original Message ----- > From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" > To: "Kim Merriam" ; "Histonet" > > Sent: Friday, October 19, 2007 11:49 AM > Subject: RE: [Histonet] NSH and Halloween > > > Aren't most of us scary enough already? > > > Jeanine Bartlett > Infectious Diseases Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim > Merriam > Sent: Friday, October 19, 2007 12:47 PM > To: Histonet > Subject: [Histonet] NSH and Halloween > > Are any of the vendors having a Halloween party at the NSH? > > Kim > > Kim Merriam, MA, HT(ASCP) > Cambridge, MA > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 18 > Date: Mon, 22 Oct 2007 16:25:33 +0100 > From: "Kemlo Rogerson" > Subject: RE: [Histonet] Video Taping > To: "Yaskovich, Ruth A \(NIH/NIDCR\) [E]" > , "histonet" > > Message-ID: > <86ADE4EB583CE64799A9924684A0FBBF0222EFC2@wahtntex2.waht.swest.nhs.uk> > Content-Type: text/plain; charset="us-ascii" > > "Are any of you (that do patient work) being video taped while at work? > This means having a camera on you while you are working in your lab. If > so, do you know if it's legal? Is it done some states versus others? I'm > posting this for a friend. > > Thanks for any help you can send our way." > Ruth > > > Depends if you are fully clothed or not . I suspect that it's legal > in the UK, maybe even obligatory! > > > Kemlo Rogerson > Pathology Manager > DD 01934 647057 or extension 3311 > Mob 07749 754194; Pager 07659 597107; > > Biology is the least of what makes someone a mother. --Oprah Winfrey > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. > Please inform me that this message has gone astray before deleting it. > Thank you for your co-operation > > > > > > ------------------------------ > > Message: 19 > Date: Mon, 22 Oct 2007 10:28:37 -0500 > From: "Cheryl R. Kerry" > Subject: from experience RE: [Histonet] Video Taping > To: "'histonet'" > Message-ID: <006401c814c0$37e6d450$6701a8c0@CHERYLSLAPTOP> > Content-Type: text/plain; charset="iso-8859-1" > > Ruth- > > I've had to walk through this quagmire before--it can be the only answer if > all other controls for safe work environments and patient care/safety have > been utilized. It sounds like your friend is approaching this with caution > and concern--good. > > Statues for this kind of issue vary wildly by state. If this is in > conjunction with a company or an established lab, they likely have an > attorney already representing them on this kind of issue. > > It's best to do it right if there is a need, and only a legal advisor > familiar with the laws of your state and the situation you're trying to > remedy can answer accurately and with the rights and best interests of all > those involved adequately addressed. It is worth the investment. > > In anticipation of being flamed--there can be situations where this is the > only answer and in doing so you PROTECT those in your lab--it is a very hard > decision to come to. > > I wish her the best. > > Cheryl > > Cheryl R. Kerry, HT(ASCP) > Full Staff Inc. > Staffing the AP Lab, one great tech at a time. > 281.852.9457 office > 281.883.7704 cell > 800.756.3309 fax and alternate phone > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Monday, October 22, 2007 9:25 AM > To: Yaskovich, Ruth A (NIH/NIDCR) [E]; histonet > Subject: Re: [Histonet] Video Taping > > Although I personally would NOT allow it, the opinions you are going to > obtain from Histonet are wrthless. > Consult with an attorney, any one will give you a "first free > consultations", use it on that question. > Ren? J. > > "Yaskovich, Ruth A (NIH/NIDCR) [E]" wrote: > Are any of you (that do patient work) being video taped while at work? > This means having a camera on you while you are working in your lab. If > so, do you know if it's legal? Is it done some states versus others? I'm > posting this for a friend. > > Thanks for any help you can send our way. > > Ruth Yaskovich > > National Institutes of Health > > Institute of Dental and Crainiofacial Research > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 20 > Date: Mon, 22 Oct 2007 16:29:20 +0100 > From: "Edwards, R.E." > Subject: RE: [Histonet] Video Taping > To: "Kemlo Rogerson" , "Yaskovich, > Ruth A (NIH/NIDCR) [E]" , "histonet" > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > I have seen the film it's called "Laboratory Wives". > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo > Rogerson > Sent: 22 October 2007 16:26 > To: Yaskovich, Ruth A (NIH/NIDCR) [E]; histonet > Subject: RE: [Histonet] Video Taping > > "Are any of you (that do patient work) being video taped while at work? > This means having a camera on you while you are working in your lab. If > so, do you know if it's legal? Is it done some states versus others? I'm > posting this for a friend. > > Thanks for any help you can send our way." > Ruth > > > Depends if you are fully clothed or not . I suspect that it's legal > in the UK, maybe even obligatory! > > > Kemlo Rogerson > Pathology Manager > DD 01934 647057 or extension 3311 > Mob 07749 754194; Pager 07659 597107; > > Biology is the least of what makes someone a mother. --Oprah Winfrey > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. > Please inform me that this message has gone astray before deleting it. > Thank you for your co-operation > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 21 > Date: Mon, 22 Oct 2007 16:31:13 +0100 > From: "Kemlo Rogerson" > Subject: RE: [Histonet] Video Taping > To: "Edwards, R.E." , "Yaskovich, Ruth A > \(NIH/NIDCR\) [E]" , "histonet" > > Message-ID: > <86ADE4EB583CE64799A9924684A0FBBF0222EFC4@wahtntex2.waht.swest.nhs.uk> > Content-Type: text/plain; charset="us-ascii" > > That's the one where they put them in a Laboratory and made them live > together for two weeks? Partly naked and with only Newcastle Brown to > drink? > > Kemlo Rogerson > Pathology Manager > DD 01934 647057 or extension 3311 > Mob 07749 754194; Pager 07659 597107; > > Biology is the least of what makes someone a mother. --Oprah Winfrey > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. > Please inform me that this message has gone astray before deleting it. > Thank you for your co-operation > > > > > > ------------------------------ > > Message: 22 > Date: Mon, 22 Oct 2007 09:33:16 -0600 > From: > Subject: RE: [Histonet] NSH and Halloween/OT world Series > To: , , > > Message-ID: <71FCC52823941D49A4BC39B48C6E3428F3BE4A@java.uchsc.edu> > Content-Type: text/plain; charset="US-ASCII" > > You won't be able to purchase tickets until 10:00 Denver time. You have > 27 minutes to wait. > Good Luck. > Pat Bell HT(ASCP) > University of Colo. Health Sciences Center > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jo Dee > Fish > Sent: Monday, October 22, 2007 9:21 AM > To: 'Molinari, Betsy'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] NSH and Halloween/OT world Series > > I have someone trying to get tickets as we speak. But, they are locked > out > of the website, it is quite frustrating!!! > > > ~~Jo Dee Fish~~ > Research Technologist III > Gladstone Institute of Cardiovascular Disease > > Telephone: (415) 734-2567 > Fax: (415) 355-0824 > E-mail: jfish@gladstone.ucsf.edu > > Mailing address: > The J. David Gladstone Institutes > 1650 Owens Street > San Francisco, CA 94158 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Molinari, > Betsy > Sent: Monday, October 22, 2007 4:26 AM > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] NSH and Halloween/OT world Series > > Is anyone interested in trying to get tickets to one of the games? Red > Sox > fan on the way. > > > Betsy Molinari HT (ASCP) > Texas Heart Institute > Cardiovascular Pathology > 6770 Bertner Ave > MC 1-283 > Houston, TX 77030 > 832-355-6524 > 832-355-6812 (fax) > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > pruegg@ihctech.net > Sent: Saturday, October 20, 2007 1:14 PM > To: 'Joe Nocito'; 'Bartlett, Jeanine (CDC/CCID/NCZVED)'; 'Kim Merriam'; > 'Histonet' > Subject: RE: [Histonet] NSH and Halloween > > If you are coming to Denver in what we call "Rocktober" dressed as a Red > Sox > or Cleveland Indian, you would be taking your life into your hands. Go > Rockies! > Patsy > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe > Nocito > Sent: Friday, October 19, 2007 6:20 PM > To: Bartlett, Jeanine (CDC/CCID/NCZVED); Kim Merriam; Histonet > Subject: Re: [Histonet] NSH and Halloween > > I'm going to dress up like a Boston Red Sox. Now, that's scary > ----- Original Message ----- > From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" > To: "Kim Merriam" ; "Histonet" > > Sent: Friday, October 19, 2007 11:49 AM > Subject: RE: [Histonet] NSH and Halloween > > > Aren't most of us scary enough already? > > > Jeanine Bartlett > Infectious Diseases Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim > Merriam > Sent: Friday, October 19, 2007 12:47 PM > To: Histonet > Subject: [Histonet] NSH and Halloween > > Are any of the vendors having a Halloween party at the NSH? > > Kim > > Kim Merriam, MA, HT(ASCP) > Cambridge, MA > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 23 > Date: Mon, 22 Oct 2007 11:51:57 -0400 > From: sheila adey > Subject: [Histonet] AEC disposal > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > Hello all, > > I'm just wondering how other institutions handle their AEC waste from the IHCs? > Sheila Adey HT MLTPort Huron HospitalMichigan > _________________________________________________________________ > Send a smile, make someone laugh, have some fun! Start now! > http://www.freemessengeremoticons.ca/?icid=EMENCA122 > > ------------------------------ > > Message: 24 > Date: Mon, 22 Oct 2007 09:01:57 -0700 (PDT) > From: Irene Cochran > Subject: [Histonet] Histotech Job Opening > To: histonet@lists.utsouthwestern.edu > Message-ID: <714212.44511.qm@web45608.mail.sp1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Experienced HT (ASCP) certified Tech needed for a full time day position at Bristol Hospital in the Central Connecticut area. > Please send resume to mnormand@bristolhospital.org > > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 47, Issue 29 > **************************************** > From Kemlo.Rogerson <@t> waht.swest.nhs.uk Wed Oct 24 07:16:01 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Oct 24 07:16:07 2007 Subject: [Histonet] Knife bevel for methacrylate sectioning? Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222EFFF@wahtntex2.waht.swest.nhs.uk> The more acute the bevel then the greater the vibration (so a 40 degree bevel ought to vibrate more than a 50 degree one). The more acute the bevel the less the friction so, as in life, it is a compromise. A compromise on the hardness of the material you are cutting with the ease of cutting. Too acute and you get 'chatters', too obtuse and it's harder to cut. I think!! Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Biology is the least of what makes someone a mother. --Oprah Winfrey This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Kemlo.Rogerson <@t> waht.swest.nhs.uk Wed Oct 24 07:17:03 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Oct 24 07:17:09 2007 Subject: [Histonet] testifying in court? Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222F000@wahtntex2.waht.swest.nhs.uk> If you are an expert in what you are telling the Court and what you tell them is the truth and well researched, why worry? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Biology is the least of what makes someone a mother. --Oprah Winfrey This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From talulahgosh <@t> gmail.com Wed Oct 24 08:31:18 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Wed Oct 24 08:31:25 2007 Subject: [Histonet] DAB GFP staining In-Reply-To: <00b701c815c3$771b8f40$a1065486@auxs.umn.edu> References: <20071023135233.fpbg3msvko0k0kgw@webmail.utoronto.ca> <00b701c815c3$771b8f40$a1065486@auxs.umn.edu> Message-ID: We have tried DAB staining with a Vectastain Elite ABC kit and a rabbit primary from Invitrogen on paraffin sections. It doesn't work at all, but we've never tried to troubleshoot it. Emily -- Yog-Sothoth knows the gate. Yog-Sothoth is the gate. Yog-Sothoth is the key and guardian of the gate. Past, present, future, all are one in Yog-Sothoth. He knows where the Old Ones broke through of old, and where They shall break through again. From gentras <@t> vetmed.auburn.edu Wed Oct 24 09:01:24 2007 From: gentras <@t> vetmed.auburn.edu (Atoska Gentry) Date: Wed Oct 24 09:01:32 2007 Subject: [Histonet] Region III seminar/conference info Message-ID: <471F5034.40507@vetmed.auburn.edu> hello, please a colleague of mine is inquiring about Region III meetings schedule, especially within the states of Alabama & Georgia. Will someone provide me with this info at your convenience? Thanks, Atoska -- Atoska S. Gentry, B.S., HT(ASCP) Research Assistant IV Scott-Ritchey RSCH Center College of Vet. Med Auburn, AL 36849 PH (334) 844-5579 FAX (334) 844-5850 email: gentras@vetmed.auburn.edu From algranth <@t> u.arizona.edu Wed Oct 24 09:12:24 2007 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Wed Oct 24 09:17:23 2007 Subject: [Histonet] testifying in court? In-Reply-To: <008f01c815c8$095b4cc0$0302a8c0@yourxhtr8hvc4p> References: <008f01c815c8$095b4cc0$0302a8c0@yourxhtr8hvc4p> Message-ID: <6.2.3.4.1.20071024065743.01f6e968@algranth.inbox.email.arizona.edu> I had to testify in court once for a murder trial about the lab's chain of custody protocol. Yes, I was nervous but it was very interesting. And, I guess the manner in which we received medical-legal specimens and stored them in a locked cabinet satisfied the lawyers and the jury. The victim came to the ER barely alive and the bullets were removed and received in the lab under the name of John Doe and then he died just as he was being identified. So later it was questioned how we knew that the bullets that I had in the lab actually came from the dead person formerly known as John Doe. That was easy and I really didn't have to explain anything about histology. I thought it was interesting that the defense lawyer took notes on a Big Chief tablet! (Younger people, you probably don't know what this is). Andi At 03:57 PM 10/23/2007, Joe Nocito wrote: >Emily, >I always feel nervous every time I give my opinion. I never had the >opportunity to testify in court, so I couldn't give you an answer. I >know, I'm a big help. > >Joe >----- Original Message ----- From: "Emily Sours" >To: >Sent: Tuesday, October 23, 2007 8:31 AM >Subject: [Histonet] testifying in court? > > >>I would like an honest opinion of the histologists, DNA technologists, etc >>(those names can appear in court trials, or those connected with you)--do >>you feel nervous offering your opinion in trial to someone's sentence? I ask >>because this is the ONLY reason I've never applied for forensic science >>positions---I adore the field, and am possibly beneficial to a PCR/DNA >>investigation. >> >>Emily >>-- >>Yog-Sothoth knows the gate. >>Yog-Sothoth is the gate. >>Yog-Sothoth is the key and guardian of the gate. Past, present, future, all >>are one in Yog-Sothoth. >>He knows where the Old Ones broke through of old, and where They shall break >>through again. >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From MadaryJ <@t> MedImmune.com Wed Oct 24 09:31:05 2007 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Wed Oct 24 09:31:38 2007 Subject: [Histonet] Joe Nocito and Testifying in court and World Series Tix during NSH Message-ID: <8F3E1865E343C943BB38506D56FF0151011E6190@MD1EV002.medimmune.com> Since I am a friend of Joe(hate to admit it), I just wonder if the rest of us can believe him when he says he is nervous about giving his opinion? I have heard many excellent lectures by Joe Nocito and will go out on a limb here and say he gives his opinion throughout the lecture and that is what we like and use to learn from experience. So at NSH can we please go out of our way and ask Joe to not give his opinion and see how long the lectures last? My bro in law lives in Denver and is trying to get tix to the world series, so if you all are wanting them, note that he is having a lard time and he lives there. Leica is giving the Art Show during the world series, I hate to miss that over a once in a lifetime world series. Nick Madary, HT/HTL(ASCP)QIHC Histology Mgr, Medimmune 301.398.6113(lab), 4745(vm),9745(fax) "To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation." From as3323 <@t> columbia.edu Wed Oct 24 10:05:37 2007 From: as3323 <@t> columbia.edu (Anna K. Schultz) Date: Wed Oct 24 10:05:40 2007 Subject: [Histonet] curious about soaking paraffin blocks. Message-ID: <471F5F41.5090606@columbia.edu> Greetings All, I remember reading on here/archives that paraffin blocks should not be soaked or kept on ice for too long--what is the reasoning behind this? I am just plain curious and still fairly new to the field. I often leave blocks on for a long time and this seems to help or at least not hurt. Thanks, Anna -- Anna K. Schultz SDRC Core A Research Technician Department of Dermatology College of Physicians and Surgeons Columbia University Vanderbilt Clinic 15-211 630 West 168^th Street New York, NY 10032 Phone: (212) 305-4954 From nrutledge <@t> CapeCodHealth.org Wed Oct 24 10:14:30 2007 From: nrutledge <@t> CapeCodHealth.org (Rutledge, Nancy) Date: Wed Oct 24 10:14:34 2007 Subject: [Histonet] Warthin-Starry, microwave Dieterle substitute? Message-ID: <47AD3B259E920D449F580E6AE82C2B8F210223@FHEXSVR2.FHDOMAIN1.capecodhealth.org> Is there a stain currently being used to replace Warthin Starry and/or Dieterle? We do VERY few and are concerned about reagent costs? Thanks. Nancy Rutledge ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org From kmerriam2003 <@t> yahoo.com Wed Oct 24 10:23:10 2007 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Wed Oct 24 10:23:19 2007 Subject: [Histonet] curious about soaking paraffin blocks. Message-ID: <546565.51719.qm@web50306.mail.re2.yahoo.com> Anna, It has been my experience that most tissues are OK if soaked for a long time; but some tissues (especially fatty ones, like brain) will swell (a lot!) when soaked for an extended period of time. Just be careful! Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA ----- Original Message ---- From: Anna K. Schultz To: histonet@lists.utsouthwestern.edu Sent: Wednesday, October 24, 2007 11:05:37 AM Subject: [Histonet] curious about soaking paraffin blocks. Greetings All, I remember reading on here/archives that paraffin blocks should not be soaked or kept on ice for too long--what is the reasoning behind this? I am just plain curious and still fairly new to the field. I often leave blocks on for a long time and this seems to help or at least not hurt. Thanks, Anna -- Anna K. Schultz SDRC Core A Research Technician Department of Dermatology College of Physicians and Surgeons Columbia University Vanderbilt Clinic 15-211 630 West 168^th Street New York, NY 10032 Phone: (212) 305-4954 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From bonnie.kaliko <@t> roche.com Wed Oct 24 10:25:46 2007 From: bonnie.kaliko <@t> roche.com (Kaliko, Bonnie) Date: Wed Oct 24 10:26:24 2007 Subject: [Histonet] curious about soaking paraffin blocks. In-Reply-To: <471F5F41.5090606@columbia.edu> References: <471F5F41.5090606@columbia.edu> Message-ID: We judge the amount of time on the ice by the tissue. The paraffin never comes into play. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anna K. Schultz Sent: Wednesday, October 24, 2007 11:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] curious about soaking paraffin blocks. Greetings All, I remember reading on here/archives that paraffin blocks should not be soaked or kept on ice for too long--what is the reasoning behind this? I am just plain curious and still fairly new to the field. I often leave blocks on for a long time and this seems to help or at least not hurt. Thanks, Anna -- Anna K. Schultz SDRC Core A Research Technician Department of Dermatology College of Physicians and Surgeons Columbia University Vanderbilt Clinic 15-211 630 West 168^th Street New York, NY 10032 Phone: (212) 305-4954 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jan.Minshew <@t> leica-microsystems.com Wed Oct 24 10:37:41 2007 From: Jan.Minshew <@t> leica-microsystems.com (Jan.Minshew@leica-microsystems.com) Date: Wed Oct 24 10:37:53 2007 Subject: [Histonet] Joe Nocito and Testifying in court and World Series Tix during NSH In-Reply-To: <8F3E1865E343C943BB38506D56FF0151011E6190@MD1EV002.medimmune.com> Message-ID: Hi, I'm also a friend of Joe's so I know he would rather be at the world series game...but I also know him well enough to know that he doesn't miss too many parties. We didn't have a clue that the game would be played in Denver when we booked our event and, believe me, a lot of our people would like to see the game too. So, if you can't get tickets to the game, you can at least find some great food, drink, entertainment and company at the Art Museum (and I feel certain that we will be able to keep up with the scores). See you at the meeting, Jan Minshew, HT(ASCP)HTL Marketing Manager Leica Microsystems, Inc. 2345 Waukegan Road Bannockburn, IL 60015 800.248.0123 x7051 toll free 847.405.7051 direct 847.405.6560 fax jan.minshew@leica-microsystems.com "Madary, Joseph" To Sent by: histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] Joe Nocito and Testifying in court and World 10/24/2007 09:31 Series Tix during NSH AM Since I am a friend of Joe(hate to admit it), I just wonder if the rest of us can believe him when he says he is nervous about giving his opinion? I have heard many excellent lectures by Joe Nocito and will go out on a limb here and say he gives his opinion throughout the lecture and that is what we like and use to learn from experience. So at NSH can we please go out of our way and ask Joe to not give his opinion and see how long the lectures last? My bro in law lives in Denver and is trying to get tix to the world series, so if you all are wanting them, note that he is having a lard time and he lives there. Leica is giving the Art Show during the world series, I hate to miss that over a once in a lifetime world series. Nick Madary, HT/HTL(ASCP)QIHC Histology Mgr, Medimmune 301.398.6113(lab), 4745(vm),9745(fax) "To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From Lynn.Burton <@t> Illinois.gov Wed Oct 24 10:50:55 2007 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Wed Oct 24 10:52:37 2007 Subject: [Histonet] curious about soaking paraffin blocks. References: <471F5F41.5090606@columbia.edu> Message-ID: I have left them on for an hour or a little more without noticing any problems. I do Chronic Wasting disease tests on deer brains. I don't notice that it effects them differently than tumors from other species. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Anna K. Schultz Sent: Wed 10/24/2007 10:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] curious about soaking paraffin blocks. Greetings All, I remember reading on here/archives that paraffin blocks should not be soaked or kept on ice for too long--what is the reasoning behind this? I am just plain curious and still fairly new to the field. I often leave blocks on for a long time and this seems to help or at least not hurt. Thanks, Anna -- Anna K. Schultz SDRC Core A Research Technician Department of Dermatology College of Physicians and Surgeons Columbia University Vanderbilt Clinic 15-211 630 West 168^th Street New York, NY 10032 Phone: (212) 305-4954 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Wanda.Smith <@t> HCAhealthcare.com Wed Oct 24 10:52:28 2007 From: Wanda.Smith <@t> HCAhealthcare.com (Smith Wanda) Date: Wed Oct 24 10:52:42 2007 Subject: [Histonet] curious about soaking paraffin blocks. In-Reply-To: <471F5F41.5090606@columbia.edu> References: <471F5F41.5090606@columbia.edu> Message-ID: <817C2761C5A1394180709AEEDB775B7E03BC5BCF@NASEV03.hca.corpad.net> Anna, The danger in leaving faced blocks on the ice too long is it causes the tissue to swell. The part of the tissue that swells will be cut away when you start to cut your sections. This can be bad for tiny biopsy specimens or needle core biopsies, because you may cut the tissue away. I hope this helps! Wanda Wanda G. Smith, HTL(ASCP)HT Trident Medical Center Pathology Supervisor ph: 843-847-4586 fx: 843-847-4296 email: wanda.smith@hcahealthcare.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anna K. Schultz Sent: Wednesday, October 24, 2007 11:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] curious about soaking paraffin blocks. Greetings All, I remember reading on here/archives that paraffin blocks should not be soaked or kept on ice for too long--what is the reasoning behind this? I am just plain curious and still fairly new to the field. I often leave blocks on for a long time and this seems to help or at least not hurt. Thanks, Anna -- Anna K. Schultz SDRC Core A Research Technician Department of Dermatology College of Physicians and Surgeons Columbia University Vanderbilt Clinic 15-211 630 West 168^th Street New York, NY 10032 Phone: (212) 305-4954 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Oct 24 10:53:03 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Oct 24 10:53:07 2007 Subject: [Histonet] Warthin-Starry, microwave Dieterle substitute? In-Reply-To: <47AD3B259E920D449F580E6AE82C2B8F210223@FHEXSVR2.FHDOMAIN1.capecodhealth.org> Message-ID: <883364.76955.qm@web61218.mail.yahoo.com> Modified Steiner with the original mordant of uranyl nitrate; much easier than Warthin Starry or Dieterle. Ren? J. "Rutledge, Nancy" wrote: Is there a stain currently being used to replace Warthin Starry and/or Dieterle? We do VERY few and are concerned about reagent costs? Thanks. Nancy Rutledge ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From LSebree <@t> uwhealth.org Wed Oct 24 11:06:18 2007 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Wed Oct 24 11:06:22 2007 Subject: [Histonet] curious about soaking paraffin blocks. In-Reply-To: <817C2761C5A1394180709AEEDB775B7E03BC5BCF@NASEV03.hca.corpad.net> Message-ID: I once left a small bm bx core to soak and the needle core soaked right out of the block never to be seen again! Now I'm more cautious depending on the tissue type and size. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith Wanda Sent: Wednesday, October 24, 2007 10:52 AM To: Anna K. Schultz; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] curious about soaking paraffin blocks. Anna, The danger in leaving faced blocks on the ice too long is it causes the tissue to swell. The part of the tissue that swells will be cut away when you start to cut your sections. This can be bad for tiny biopsy specimens or needle core biopsies, because you may cut the tissue away. I hope this helps! Wanda Wanda G. Smith, HTL(ASCP)HT Trident Medical Center Pathology Supervisor ph: 843-847-4586 fx: 843-847-4296 email: wanda.smith@hcahealthcare.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anna K. Schultz Sent: Wednesday, October 24, 2007 11:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] curious about soaking paraffin blocks. Greetings All, I remember reading on here/archives that paraffin blocks should not be soaked or kept on ice for too long--what is the reasoning behind this? I am just plain curious and still fairly new to the field. I often leave blocks on for a long time and this seems to help or at least not hurt. Thanks, Anna -- Anna K. Schultz SDRC Core A Research Technician Department of Dermatology College of Physicians and Surgeons Columbia University Vanderbilt Clinic 15-211 630 West 168^th Street New York, NY 10032 Phone: (212) 305-4954 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From settembr <@t> umdnj.edu Wed Oct 24 11:12:45 2007 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Wed Oct 24 11:13:26 2007 Subject: [Histonet] Big Chief tablet? Message-ID: What's a "Big Chief tablet"? Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Andrea Grantham 10/24/07 10:12 AM >>> I had to testify in court once for a murder trial about the lab's chain of custody protocol. Yes, I was nervous but it was very interesting. And, I guess the manner in which we received medical-legal specimens and stored them in a locked cabinet satisfied the lawyers and the jury. The victim came to the ER barely alive and the bullets were removed and received in the lab under the name of John Doe and then he died just as he was being identified. So later it was questioned how we knew that the bullets that I had in the lab actually came from the dead person formerly known as John Doe. That was easy and I really didn't have to explain anything about histology. I thought it was interesting that the defense lawyer took notes on a Big Chief tablet! (Younger people, you probably don't know what this is). Andi At 03:57 PM 10/23/2007, Joe Nocito wrote: >Emily, >I always feel nervous every time I give my opinion. I never had the >opportunity to testify in court, so I couldn't give you an answer. I >know, I'm a big help. > >Joe >----- Original Message ----- From: "Emily Sours" >To: >Sent: Tuesday, October 23, 2007 8:31 AM >Subject: [Histonet] testifying in court? > > >>I would like an honest opinion of the histologists, DNA technologists, etc >>(those names can appear in court trials, or those connected with you)--do >>you feel nervous offering your opinion in trial to someone's sentence? I ask >>because this is the ONLY reason I've never applied for forensic science >>positions---I adore the field, and am possibly beneficial to a PCR/DNA >>investigation. >> >>Emily >>-- >>Yog-Sothoth knows the gate. >>Yog-Sothoth is the gate. >>Yog-Sothoth is the key and guardian of the gate. Past, present, future, all >>are one in Yog-Sothoth. >>He knows where the Old Ones broke through of old, and where They shall break >>through again. >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alaskagirl1950 <@t> yahoo.com Wed Oct 24 11:24:16 2007 From: alaskagirl1950 <@t> yahoo.com (Patricia Adams) Date: Wed Oct 24 11:24:19 2007 Subject: [Histonet] Big Chief tablet? In-Reply-To: Message-ID: <313636.7770.qm@web52503.mail.re2.yahoo.com> Reminds me of going through some old records and my son, than 5 asked where the giant CD's came from. He is now 17. Patricia Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From rjbuesa <@t> yahoo.com Wed Oct 24 11:42:38 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Oct 24 11:42:43 2007 Subject: [Histonet] Big Chief tablet? In-Reply-To: <313636.7770.qm@web52503.mail.re2.yahoo.com> Message-ID: <971567.61509.qm@web61223.mail.yahoo.com> It reminds me of a very good old joke about a constipated Big Chief, but it is not suitable to post on this forum. Ren? J. Patricia Adams wrote: Reminds me of going through some old records and my son, than 5 asked where the giant CD's came from. He is now 17. Patricia Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From jqb7 <@t> CDC.GOV Wed Oct 24 11:43:24 2007 From: jqb7 <@t> CDC.GOV (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed Oct 24 11:43:40 2007 Subject: [Histonet] Big Chief tablet? In-Reply-To: References: Message-ID: <34BB307EFC9A65429BBB49E330675F72045E20F1@LTA3VS003.ees.hhs.gov> For the youngsters out there---------------- The Big Chief tablet was a popular writing notebook for several generations of young children in the United States. It featured widely spaced lines, easier to write in for those learning to write. Its prominence, though, was the cover's representation of a native American in full headdress, hence "Big Chief." Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dana Settembre Sent: Wednesday, October 24, 2007 12:13 PM To: histonet@lists.utsouthwestern.edu; Andrea Grantham Subject: Re: [Histonet] Big Chief tablet? What's a "Big Chief tablet"? Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Andrea Grantham 10/24/07 10:12 AM >>> I had to testify in court once for a murder trial about the lab's chain of custody protocol. Yes, I was nervous but it was very interesting. And, I guess the manner in which we received medical-legal specimens and stored them in a locked cabinet satisfied the lawyers and the jury. The victim came to the ER barely alive and the bullets were removed and received in the lab under the name of John Doe and then he died just as he was being identified. So later it was questioned how we knew that the bullets that I had in the lab actually came from the dead person formerly known as John Doe. That was easy and I really didn't have to explain anything about histology. I thought it was interesting that the defense lawyer took notes on a Big Chief tablet! (Younger people, you probably don't know what this is). Andi At 03:57 PM 10/23/2007, Joe Nocito wrote: >Emily, >I always feel nervous every time I give my opinion. I never had the >opportunity to testify in court, so I couldn't give you an answer. I >know, I'm a big help. > >Joe >----- Original Message ----- From: "Emily Sours" >To: >Sent: Tuesday, October 23, 2007 8:31 AM >Subject: [Histonet] testifying in court? > > >>I would like an honest opinion of the histologists, DNA technologists, etc >>(those names can appear in court trials, or those connected with you)--do >>you feel nervous offering your opinion in trial to someone's sentence? I ask >>because this is the ONLY reason I've never applied for forensic science >>positions---I adore the field, and am possibly beneficial to a PCR/DNA >>investigation. >> >>Emily >>-- >>Yog-Sothoth knows the gate. >>Yog-Sothoth is the gate. >>Yog-Sothoth is the key and guardian of the gate. Past, present, future, all >>are one in Yog-Sothoth. >>He knows where the Old Ones broke through of old, and where They shall break >>through again. >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BMolinari <@t> heart.thi.tmc.edu Wed Oct 24 12:02:33 2007 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Wed Oct 24 12:02:39 2007 Subject: [Histonet] Joe Nocito and Testifying in court and World Series Tixduring NSH In-Reply-To: <8F3E1865E343C943BB38506D56FF0151011E6190@MD1EV002.medimmune.com> Message-ID: Nick, I had 5 people trying yesterday..no one had any luck. I will remain optimistic that somehow..somewhere a ticket will become available ..after all I am a Red Sox fan..I have had decades of hoping. Betsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Madary, Joseph Sent: Wednesday, October 24, 2007 9:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Joe Nocito and Testifying in court and World Series Tixduring NSH Since I am a friend of Joe(hate to admit it), I just wonder if the rest of us can believe him when he says he is nervous about giving his opinion? I have heard many excellent lectures by Joe Nocito and will go out on a limb here and say he gives his opinion throughout the lecture and that is what we like and use to learn from experience. So at NSH can we please go out of our way and ask Joe to not give his opinion and see how long the lectures last? My bro in law lives in Denver and is trying to get tix to the world series, so if you all are wanting them, note that he is having a lard time and he lives there. Leica is giving the Art Show during the world series, I hate to miss that over a once in a lifetime world series. Nick Madary, HT/HTL(ASCP)QIHC Histology Mgr, Medimmune 301.398.6113(lab), 4745(vm),9745(fax) "To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jfish <@t> gladstone.ucsf.edu Wed Oct 24 12:22:12 2007 From: jfish <@t> gladstone.ucsf.edu (Jo Dee Fish) Date: Wed Oct 24 12:23:29 2007 Subject: [Histonet] Joe Nocito and Testifying in court and World SeriesTixduring NSH In-Reply-To: Message-ID: <000e01c81662$6a186990$2e0d010a@JFISH> I actually had a dream last night that a stranger on the street bought 4, couldn't sell 2, and just gave them to me (like that would happen). I guess after trying for 2 days to buy them (it's my mom's birthday and I thought it would be a great experience for her)I still haven't given up hope! Jo Dee ~~Jo Dee Fish~~ Research Technologist III Gladstone Institute of Cardiovascular Disease Telephone: (415) 734-2567 Fax: (415) 355-0824 E-mail: jfish@gladstone.ucsf.edu Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Wednesday, October 24, 2007 10:03 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Joe Nocito and Testifying in court and World SeriesTixduring NSH Nick, I had 5 people trying yesterday..no one had any luck. I will remain optimistic that somehow..somewhere a ticket will become available ..after all I am a Red Sox fan..I have had decades of hoping. Betsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Madary, Joseph Sent: Wednesday, October 24, 2007 9:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Joe Nocito and Testifying in court and World Series Tixduring NSH Since I am a friend of Joe(hate to admit it), I just wonder if the rest of us can believe him when he says he is nervous about giving his opinion? I have heard many excellent lectures by Joe Nocito and will go out on a limb here and say he gives his opinion throughout the lecture and that is what we like and use to learn from experience. So at NSH can we please go out of our way and ask Joe to not give his opinion and see how long the lectures last? My bro in law lives in Denver and is trying to get tix to the world series, so if you all are wanting them, note that he is having a lard time and he lives there. Leica is giving the Art Show during the world series, I hate to miss that over a once in a lifetime world series. Nick Madary, HT/HTL(ASCP)QIHC Histology Mgr, Medimmune 301.398.6113(lab), 4745(vm),9745(fax) "To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Wed Oct 24 12:23:46 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Wed Oct 24 12:25:55 2007 Subject: [Histonet] Re: DAB GFP staining Message-ID: <003001c81662$d28fe1b0$4101a8c0@carlba65530bda> Hi Alonso. Do you mean using DAB as a chromogen after detecting GFP with an anti -GFP antibody reagent? Also, what type of tissue preparation are you using? If you look here : http://www.immunoportal.com/index.php in the Image gallery, you will see some pictures of GFP/ anti-GFP in pwax sections. Carl From carl.hobbs <@t> kcl.ac.uk Wed Oct 24 12:29:19 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Wed Oct 24 12:29:42 2007 Subject: [Histonet] alternative IHC marker other than GFAP for Message-ID: <003401c81663$69367890$4101a8c0@carlba65530bda> Why do you want an alternative to GFAP as a marker of Astrocytes? I would be grateful for your answer. Thanks Carl From slappycraw <@t> yahoo.com Wed Oct 24 12:29:41 2007 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Wed Oct 24 12:29:48 2007 Subject: [Histonet] Big Chief tablet? In-Reply-To: <971567.61509.qm@web61223.mail.yahoo.com> Message-ID: <739407.68207.qm@web53610.mail.re2.yahoo.com> Is that the joke where the Chief can't move because? Rene J Buesa wrote: It reminds me of a very good old joke about a constipated Big Chief, but it is not suitable to post on this forum. Ren? J. Patricia Adams wrote: Reminds me of going through some old records and my son, than 5 asked where the giant CD's came from. He is now 17. Patricia Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Larry A. Woody Amgen Seattle, Wa. __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From bbroders <@t> unlnotes.unl.edu Wed Oct 24 12:53:04 2007 From: bbroders <@t> unlnotes.unl.edu (Bruce W Brodersen) Date: Wed Oct 24 12:53:15 2007 Subject: [Histonet] Fast red washing off Message-ID: Anyone have trouble with fast red substrate washing off slides during slide dehydration for coverslipping? Even though the label claims on two different Fast Reds we have say they can go through alcohols to xylene we see there is loss of the substrate. Thanks. Bruce W. Brodersen, DVM, PhD University of Nebraska Veterinary Diagnostic Center Fair St. & E. Campus Loop Lincoln, NE 68583-0907 voice (402) 472-1434 FAX (402 472-3094 From b-frederick <@t> northwestern.edu Wed Oct 24 13:12:14 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Oct 24 13:12:27 2007 Subject: [Histonet] Warthin-Starry, microwave Dieterle substitute? In-Reply-To: <47AD3B259E920D449F580E6AE82C2B8F210223@FHEXSVR2.FHDOMAIN1.capecodhealth.org> Message-ID: <001801c81669$6b167470$d00f7ca5@lurie.northwestern.edu> Sigma sells a modified steiner kit. Can be used standard as well as microwave. Ventana has one for the Nexus. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rutledge, Nancy Sent: Wednesday, October 24, 2007 10:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Warthin-Starry, microwave Dieterle substitute? Is there a stain currently being used to replace Warthin Starry and/or Dieterle? We do VERY few and are concerned about reagent costs? Thanks. Nancy Rutledge ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From settembr <@t> umdnj.edu Wed Oct 24 13:55:57 2007 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Wed Oct 24 13:56:48 2007 Subject: [Histonet] Big Chief tablet? Message-ID: I am not a youngster. I am in my 40's and thank you for telling me what the Big chief tablet is. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> "Bartlett, Jeanine (CDC/CCID/NCZVED)" 10/24/07 12:43 PM >>> For the youngsters out there---------------- The Big Chief tablet was a popular writing notebook for several generations of young children in the United States. It featured widely spaced lines, easier to write in for those learning to write. Its prominence, though, was the cover's representation of a native American in full headdress, hence "Big Chief." Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dana Settembre Sent: Wednesday, October 24, 2007 12:13 PM To: histonet@lists.utsouthwestern.edu; Andrea Grantham Subject: Re: [Histonet] Big Chief tablet? What's a "Big Chief tablet"? Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Andrea Grantham 10/24/07 10:12 AM >>> I had to testify in court once for a murder trial about the lab's chain of custody protocol. Yes, I was nervous but it was very interesting. And, I guess the manner in which we received medical-legal specimens and stored them in a locked cabinet satisfied the lawyers and the jury. The victim came to the ER barely alive and the bullets were removed and received in the lab under the name of John Doe and then he died just as he was being identified. So later it was questioned how we knew that the bullets that I had in the lab actually came from the dead person formerly known as John Doe. That was easy and I really didn't have to explain anything about histology. I thought it was interesting that the defense lawyer took notes on a Big Chief tablet! (Younger people, you probably don't know what this is). Andi At 03:57 PM 10/23/2007, Joe Nocito wrote: >Emily, >I always feel nervous every time I give my opinion. I never had the >opportunity to testify in court, so I couldn't give you an answer. I >know, I'm a big help. > >Joe >----- Original Message ----- From: "Emily Sours" >To: >Sent: Tuesday, October 23, 2007 8:31 AM >Subject: [Histonet] testifying in court? > > >>I would like an honest opinion of the histologists, DNA technologists, etc >>(those names can appear in court trials, or those connected with you)--do >>you feel nervous offering your opinion in trial to someone's sentence? I ask >>because this is the ONLY reason I've never applied for forensic science >>positions---I adore the field, and am possibly beneficial to a PCR/DNA >>investigation. >> >>Emily >>-- >>Yog-Sothoth knows the gate. >>Yog-Sothoth is the gate. >>Yog-Sothoth is the key and guardian of the gate. Past, present, future, all >>are one in Yog-Sothoth. >>He knows where the Old Ones broke through of old, and where They shall break >>through again. >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Wed Oct 24 14:02:02 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Oct 24 14:02:41 2007 Subject: [Histonet] Big Chief tablet? In-Reply-To: Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F4006@sjhaexc02.sjha.org> Honey, you are a mere child...j:>) Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Dana Settembre Sent: Wednesday, October 24, 2007 2:56 PM To: Jeanine (CDC/CCID/NCZVED) Bartlett; histonet@lists.utsouthwestern.edu; Andrea Grantham Subject: RE: [Histonet] Big Chief tablet? I am not a youngster. I am in my 40's and thank you for telling me what the Big chief tablet is. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> "Bartlett, Jeanine (CDC/CCID/NCZVED)" 10/24/07 12:43 PM >>> For the youngsters out there---------------- The Big Chief tablet was a popular writing notebook for several generations of young children in the United States. It featured widely spaced lines, easier to write in for those learning to write. Its prominence, though, was the cover's representation of a native American in full headdress, hence "Big Chief." Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dana Settembre Sent: Wednesday, October 24, 2007 12:13 PM To: histonet@lists.utsouthwestern.edu; Andrea Grantham Subject: Re: [Histonet] Big Chief tablet? What's a "Big Chief tablet"? Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Andrea Grantham 10/24/07 10:12 AM >>> I had to testify in court once for a murder trial about the lab's chain of custody protocol. Yes, I was nervous but it was very interesting. And, I guess the manner in which we received medical-legal specimens and stored them in a locked cabinet satisfied the lawyers and the jury. The victim came to the ER barely alive and the bullets were removed and received in the lab under the name of John Doe and then he died just as he was being identified. So later it was questioned how we knew that the bullets that I had in the lab actually came from the dead person formerly known as John Doe. That was easy and I really didn't have to explain anything about histology. I thought it was interesting that the defense lawyer took notes on a Big Chief tablet! (Younger people, you probably don't know what this is). Andi At 03:57 PM 10/23/2007, Joe Nocito wrote: >Emily, >I always feel nervous every time I give my opinion. I never had the >opportunity to testify in court, so I couldn't give you an answer. I >know, I'm a big help. > >Joe >----- Original Message ----- From: "Emily Sours" >To: >Sent: Tuesday, October 23, 2007 8:31 AM >Subject: [Histonet] testifying in court? > > >>I would like an honest opinion of the histologists, DNA technologists, etc >>(those names can appear in court trials, or those connected with you)--do >>you feel nervous offering your opinion in trial to someone's sentence? I ask >>because this is the ONLY reason I've never applied for forensic science >>positions---I adore the field, and am possibly beneficial to a PCR/DNA >>investigation. >> >>Emily >>-- >>Yog-Sothoth knows the gate. >>Yog-Sothoth is the gate. >>Yog-Sothoth is the key and guardian of the gate. Past, present, future, all >>are one in Yog-Sothoth. >>He knows where the Old Ones broke through of old, and where They shall break >>through again. >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From ander093 <@t> tc.umn.edu Wed Oct 24 14:12:30 2007 From: ander093 <@t> tc.umn.edu (LuAnn Anderson) Date: Wed Oct 24 14:12:49 2007 Subject: [Histonet] Big Chief tablet? In-Reply-To: References: Message-ID: Yup--still a youngster. lol At 01:55 PM 10/24/2007, Dana Settembre wrote: >I am not a youngster. I am in my 40's and thank you for >telling me what the Big chief tablet is. > > >Dana Settembre, HT ASCP >Immunohistochemistry Lab >UMDNJ - University Hospital >Newark, NJ USA > > > >>> "Bartlett, Jeanine (CDC/CCID/NCZVED)" 10/24/07 12:43 >PM >>> >For the youngsters out there---------------- > > The Big Chief tablet was a popular writing notebook for several >generations of young children in the United States. It featured widely >spaced lines, easier to write in for those learning to write. Its >prominence, though, was the cover's representation of a native >American >in full headdress, hence "Big Chief." > > > >Jeanine Bartlett >Infectious Diseases Pathology Branch >(404) 639-3590 >jeanine.bartlett@cdc.hhs.gov > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dana >Settembre >Sent: Wednesday, October 24, 2007 12:13 PM >To: histonet@lists.utsouthwestern.edu; Andrea Grantham >Subject: Re: [Histonet] Big Chief tablet? > >What's a "Big Chief tablet"? > > >Dana Settembre, HT ASCP >Immunohistochemistry Lab >UMDNJ - University Hospital >Newark, NJ USA > > > >>> Andrea Grantham 10/24/07 10:12 AM >>> >I had to testify in court once for a murder trial about the lab's >chain >of custody protocol. Yes, I was nervous but it was very interesting. >And, I guess the manner in which we received medical-legal specimens >and >stored them in a locked cabinet satisfied the lawyers and the jury. >The >victim came to the ER barely alive and the bullets were removed and >received in the lab under the name of John Doe and then he died just >as >he was being identified. So later it was questioned how we knew that >the >bullets that I had in the lab actually came from the dead person >formerly known as John Doe. That was easy and I really didn't have to >explain anything about histology. >I thought it was interesting that the defense lawyer took notes on a >Big >Chief tablet! (Younger people, you probably don't know what this is). > >Andi > > > >At 03:57 PM 10/23/2007, Joe Nocito wrote: > >Emily, > >I always feel nervous every time I give my opinion. I never had the > >opportunity to testify in court, so I couldn't give you an answer. I > >know, I'm a big help. > > > >Joe > >----- Original Message ----- From: "Emily Sours" > > >To: > >Sent: Tuesday, October 23, 2007 8:31 AM > >Subject: [Histonet] testifying in court? > > > > > >>I would like an honest opinion of the histologists, DNA >technologists, etc > >>(those names can appear in court trials, or those connected with >you)--do > >>you feel nervous offering your opinion in trial to someone's >sentence? I ask > >>because this is the ONLY reason I've never applied for forensic >science > >>positions---I adore the field, and am possibly beneficial to a >PCR/DNA > >>investigation. > >> > >>Emily > >>-- > >>Yog-Sothoth knows the gate. > >>Yog-Sothoth is the gate. > >>Yog-Sothoth is the key and guardian of the gate. Past, present, >future, all > >>are one in Yog-Sothoth. > >>He knows where the Old Ones broke through of old, and where They >shall break > >>through again. > >>_______________________________________________ > >>Histonet mailing list > >>Histonet@lists.utsouthwestern.edu > >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >..................................................................... >: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : >: Sr. Research Specialist University of Arizona : >: (office: AHSC 4212) P.O. Box 245044 : >: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : >: (FAX: 520-626-2097) (email: algranth@u.arizona.edu) >: >:...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mannis <@t> usgs.gov Wed Oct 24 14:31:16 2007 From: mannis <@t> usgs.gov (Mandy Annis) Date: Wed Oct 24 14:31:26 2007 Subject: [Histonet] free equipment Message-ID: We recently purchased 2 new microtomes and a cryostat (with the help of histonet equipment suggestions), and would like to see our old work horses receive new homes. We have two vintage AO 820 microtomes and a slightly newer Damon/ International Equipment Company Minotome (with Minot microtome) that are available for only the price of shipping. All are in relatively good shape, with the microtomes having been serviced recently. If anyone is interested in this equipment or parts of the equipment please contact me by the end of next week or the next stop for these relics will be the recycle yard! ********************************************************************** Mandy L. Annis Columbia Environmental Research Center 4200 New Haven RD Columbia, MO 65201 Phone:(573)-441-2940 Fax:(573)-876-1896 e-mail:mannis@usgs.gov From MBURTON1 <@t> PARTNERS.ORG Wed Oct 24 14:45:18 2007 From: MBURTON1 <@t> PARTNERS.ORG (Burton, Mark) Date: Wed Oct 24 14:45:27 2007 Subject: [Histonet] Warthin-Starry, microwave Dieterle substitute? In-Reply-To: <001801c81669$6b167470$d00f7ca5@lurie.northwestern.edu> Message-ID: I would be cautious before using Nexus for Steiner stains. I would do several comparison runs against a manual Steiner with well known controls and different types of controls if you have them available. I used to purchase a spirochete control form Newcomer and we had a Bartonella control that we made. Mark Burton HTL ASCP Brigham & Women's Hospital Boston, MA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Wednesday, October 24, 2007 2:12 PM To: 'Rutledge, Nancy'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Warthin-Starry, microwave Dieterle substitute? Sigma sells a modified steiner kit. Can be used standard as well as microwave. Ventana has one for the Nexus. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rutledge, Nancy Sent: Wednesday, October 24, 2007 10:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Warthin-Starry, microwave Dieterle substitute? Is there a stain currently being used to replace Warthin Starry and/or Dieterle? We do VERY few and are concerned about reagent costs? Thanks. Nancy Rutledge ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From b-frederick <@t> northwestern.edu Wed Oct 24 15:19:38 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Oct 24 15:20:06 2007 Subject: [Histonet] Warthin-Starry, microwave Dieterle substitute? In-Reply-To: Message-ID: <002401c8167b$36dcfd20$d00f7ca5@lurie.northwestern.edu> All, Sigma also sells controls as well as their kit. I've used it in the microwave. I actually had to perfect the kit on smears from the VD clinic. Fat chubby criiters let me tell you! At least they were highly visible. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Burton, Mark Sent: Wednesday, October 24, 2007 2:45 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Warthin-Starry, microwave Dieterle substitute? I would be cautious before using Nexus for Steiner stains. I would do several comparison runs against a manual Steiner with well known controls and different types of controls if you have them available. I used to purchase a spirochete control form Newcomer and we had a Bartonella control that we made. Mark Burton HTL ASCP Brigham & Women's Hospital Boston, MA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Wednesday, October 24, 2007 2:12 PM To: 'Rutledge, Nancy'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Warthin-Starry, microwave Dieterle substitute? Sigma sells a modified steiner kit. Can be used standard as well as microwave. Ventana has one for the Nexus. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rutledge, Nancy Sent: Wednesday, October 24, 2007 10:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Warthin-Starry, microwave Dieterle substitute? Is there a stain currently being used to replace Warthin Starry and/or Dieterle? We do VERY few and are concerned about reagent costs? Thanks. Nancy Rutledge ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Thu Oct 25 02:05:31 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Oct 25 02:05:40 2007 Subject: [Histonet] curious about soaking paraffin blocks. Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222F00A@wahtntex2.waht.swest.nhs.uk> "Greetings All, I remember reading on here/archives that paraffin blocks should not be soaked or kept on ice for too long--what is the reasoning behind this? I am just plain curious and still fairly new to the field. I often leave blocks on for a long time and this seems to help or at least not hurt. Thanks, Anna" Because you've taken all that time and energy to get the water out, putting them on ice after they've been trimmed allows water to re-enter. Leave a cut block in water for 2-3 days; it gets very wet. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Biology is the least of what makes someone a mother. --Oprah Winfrey This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From christina_bisgaard <@t> yahoo.dk Thu Oct 25 05:06:21 2007 From: christina_bisgaard <@t> yahoo.dk (christina bisgaard) Date: Thu Oct 25 05:06:28 2007 Subject: [Histonet] Please remove me from the list Message-ID: <578154.48493.qm@web54410.mail.yahoo.com> Please remove me from the list --------------------------------- Tr?nger du til at se det store billede? Kelkoo giver dig gode tilbud p? LCD TV! From jnocito <@t> satx.rr.com Thu Oct 25 05:47:57 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Oct 25 05:48:00 2007 Subject: [Histonet] Denver Message-ID: <003601c816f4$81b0fb70$0302a8c0@yourxhtr8hvc4p> The Toe is ready. I have my thermal socks ready too. Have a safe flight to Denver for those who can go. JTT From godsgalnow <@t> aol.com Thu Oct 25 06:56:13 2007 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Thu Oct 25 06:56:30 2007 Subject: [Histonet] Fast red washing off In-Reply-To: References: Message-ID: <8C9E5152D4711DA-114-3B96@webmail-da04.sysops.aol.com> Bruce, We never run the fast red through alcohol or xylene.? After the slides are finished we rinse them in water and allow them to air dry....(if you are in a big hurry, you can put them in the slide drying oven to speed this up a bit).? To coverslip them, dip in slide?slidebrite and mount with permount.? This should fix the problem. Roxanne Soto HT(ASCP)QIHC Lab Manager Tampa, FL -----Original Message----- From: Bruce W Brodersen To: histonet@lists.utsouthwestern.edu Sent: Wed, 24 Oct 2007 1:53 pm Subject: [Histonet] Fast red washing off Anyone have trouble with fast red substrate washing off slides during slide dehydration for coverslipping? Even though the label claims on two different Fast Reds we have say they can go through alcohols to xylene we see there is loss of the substrate. Thanks. Bruce W. Brodersen, DVM, PhD University of Nebraska Veterinary Diagnostic Center Fair St. & E. Campus Loop Lincoln, NE 68583-0907 voice (402) 472-1434 FAX (402 472-3094_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ Email and AIM finally together. You've gotta check out free AOL Mail! - http://mail.aol.com From cmiller <@t> physlab.com Thu Oct 25 08:33:39 2007 From: cmiller <@t> physlab.com (Cheri Miller) Date: Thu Oct 25 08:33:45 2007 Subject: [Histonet] curious about soaking paraffin blocks. In-Reply-To: <817C2761C5A1394180709AEEDB775B7E03BC5BCF@NASEV03.hca.corpad.net> References: <471F5F41.5090606@columbia.edu> <817C2761C5A1394180709AEEDB775B7E03BC5BCF@NASEV03.hca.corpad.net> Message-ID: <005d01c8170b$a6fb5f30$3402a8c0@plab.local> My experience shows soaking sometimes facilitates better sectioning it just depends on the tissue. When I do soak blocks, the small biopsies I place on the ice tissue side up so I don't risk swelling. Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith Wanda Sent: Wednesday, October 24, 2007 10:52 AM To: Anna K. Schultz; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] curious about soaking paraffin blocks. Anna, The danger in leaving faced blocks on the ice too long is it causes the tissue to swell. The part of the tissue that swells will be cut away when you start to cut your sections. This can be bad for tiny biopsy specimens or needle core biopsies, because you may cut the tissue away. I hope this helps! Wanda Wanda G. Smith, HTL(ASCP)HT Trident Medical Center Pathology Supervisor ph: 843-847-4586 fx: 843-847-4296 email: wanda.smith@hcahealthcare.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anna K. Schultz Sent: Wednesday, October 24, 2007 11:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] curious about soaking paraffin blocks. Greetings All, I remember reading on here/archives that paraffin blocks should not be soaked or kept on ice for too long--what is the reasoning behind this? I am just plain curious and still fairly new to the field. I often leave blocks on for a long time and this seems to help or at least not hurt. Thanks, Anna -- Anna K. Schultz SDRC Core A Research Technician Department of Dermatology College of Physicians and Surgeons Columbia University Vanderbilt Clinic 15-211 630 West 168^th Street New York, NY 10032 Phone: (212) 305-4954 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From cmiller <@t> physlab.com Thu Oct 25 08:39:55 2007 From: cmiller <@t> physlab.com (Cheri Miller) Date: Thu Oct 25 08:40:01 2007 Subject: [Histonet] Big Chief tablet? In-Reply-To: <34BB307EFC9A65429BBB49E330675F72045E20F1@LTA3VS003.ees.hhs.gov> References: <34BB307EFC9A65429BBB49E330675F72045E20F1@LTA3VS003.ees.hhs.gov> Message-ID: <005e01c8170c$87356910$3402a8c0@plab.local> I loved Big Cheif! When my daughter was young (she is 19 now) I looked for the Big Chief tablet so I could teach her to write her numbers/letters before she started school but I never did find it. Cheri Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Wednesday, October 24, 2007 11:43 AM To: Dana Settembre; histonet@lists.utsouthwestern.edu; Andrea Grantham Subject: RE: [Histonet] Big Chief tablet? For the youngsters out there---------------- The Big Chief tablet was a popular writing notebook for several generations of young children in the United States. It featured widely spaced lines, easier to write in for those learning to write. Its prominence, though, was the cover's representation of a native American in full headdress, hence "Big Chief." Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dana Settembre Sent: Wednesday, October 24, 2007 12:13 PM To: histonet@lists.utsouthwestern.edu; Andrea Grantham Subject: Re: [Histonet] Big Chief tablet? What's a "Big Chief tablet"? Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Andrea Grantham 10/24/07 10:12 AM >>> I had to testify in court once for a murder trial about the lab's chain of custody protocol. Yes, I was nervous but it was very interesting. And, I guess the manner in which we received medical-legal specimens and stored them in a locked cabinet satisfied the lawyers and the jury. The victim came to the ER barely alive and the bullets were removed and received in the lab under the name of John Doe and then he died just as he was being identified. So later it was questioned how we knew that the bullets that I had in the lab actually came from the dead person formerly known as John Doe. That was easy and I really didn't have to explain anything about histology. I thought it was interesting that the defense lawyer took notes on a Big Chief tablet! (Younger people, you probably don't know what this is). Andi At 03:57 PM 10/23/2007, Joe Nocito wrote: >Emily, >I always feel nervous every time I give my opinion. I never had the >opportunity to testify in court, so I couldn't give you an answer. I >know, I'm a big help. > >Joe >----- Original Message ----- From: "Emily Sours" >To: >Sent: Tuesday, October 23, 2007 8:31 AM >Subject: [Histonet] testifying in court? > > >>I would like an honest opinion of the histologists, DNA technologists, etc >>(those names can appear in court trials, or those connected with you)--do >>you feel nervous offering your opinion in trial to someone's sentence? I ask >>because this is the ONLY reason I've never applied for forensic science >>positions---I adore the field, and am possibly beneficial to a PCR/DNA >>investigation. >> >>Emily >>-- >>Yog-Sothoth knows the gate. >>Yog-Sothoth is the gate. >>Yog-Sothoth is the key and guardian of the gate. Past, present, future, all >>are one in Yog-Sothoth. >>He knows where the Old Ones broke through of old, and where They shall break >>through again. >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From cmiller <@t> physlab.com Thu Oct 25 08:42:20 2007 From: cmiller <@t> physlab.com (Cheri Miller) Date: Thu Oct 25 08:42:24 2007 Subject: [Histonet] Big Chief tablet? In-Reply-To: References: Message-ID: <005f01c8170c$dd6c0730$3402a8c0@plab.local> I'm 47 and I remember Big Cheif Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LuAnn Anderson Sent: Wednesday, October 24, 2007 2:13 PM To: Dana Settembre; Jeanine (CDC/CCID/NCZVED) Bartlett; histonet@lists.utsouthwestern.edu; Andrea Grantham Subject: RE: [Histonet] Big Chief tablet? Yup--still a youngster. lol At 01:55 PM 10/24/2007, Dana Settembre wrote: >I am not a youngster. I am in my 40's and thank you for >telling me what the Big chief tablet is. > > >Dana Settembre, HT ASCP >Immunohistochemistry Lab >UMDNJ - University Hospital >Newark, NJ USA > > > >>> "Bartlett, Jeanine (CDC/CCID/NCZVED)" 10/24/07 12:43 >PM >>> >For the youngsters out there---------------- > > The Big Chief tablet was a popular writing notebook for several >generations of young children in the United States. It featured widely >spaced lines, easier to write in for those learning to write. Its >prominence, though, was the cover's representation of a native >American >in full headdress, hence "Big Chief." > > > >Jeanine Bartlett >Infectious Diseases Pathology Branch >(404) 639-3590 >jeanine.bartlett@cdc.hhs.gov > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dana >Settembre >Sent: Wednesday, October 24, 2007 12:13 PM >To: histonet@lists.utsouthwestern.edu; Andrea Grantham >Subject: Re: [Histonet] Big Chief tablet? > >What's a "Big Chief tablet"? > > >Dana Settembre, HT ASCP >Immunohistochemistry Lab >UMDNJ - University Hospital >Newark, NJ USA > > > >>> Andrea Grantham 10/24/07 10:12 AM >>> >I had to testify in court once for a murder trial about the lab's >chain >of custody protocol. Yes, I was nervous but it was very interesting. >And, I guess the manner in which we received medical-legal specimens >and >stored them in a locked cabinet satisfied the lawyers and the jury. >The >victim came to the ER barely alive and the bullets were removed and >received in the lab under the name of John Doe and then he died just >as >he was being identified. So later it was questioned how we knew that >the >bullets that I had in the lab actually came from the dead person >formerly known as John Doe. That was easy and I really didn't have to >explain anything about histology. >I thought it was interesting that the defense lawyer took notes on a >Big >Chief tablet! (Younger people, you probably don't know what this is). > >Andi > > > >At 03:57 PM 10/23/2007, Joe Nocito wrote: > >Emily, > >I always feel nervous every time I give my opinion. I never had the > >opportunity to testify in court, so I couldn't give you an answer. I > >know, I'm a big help. > > > >Joe > >----- Original Message ----- From: "Emily Sours" > > >To: > >Sent: Tuesday, October 23, 2007 8:31 AM > >Subject: [Histonet] testifying in court? > > > > > >>I would like an honest opinion of the histologists, DNA >technologists, etc > >>(those names can appear in court trials, or those connected with >you)--do > >>you feel nervous offering your opinion in trial to someone's >sentence? I ask > >>because this is the ONLY reason I've never applied for forensic >science > >>positions---I adore the field, and am possibly beneficial to a >PCR/DNA > >>investigation. > >> > >>Emily > >>-- > >>Yog-Sothoth knows the gate. > >>Yog-Sothoth is the gate. > >>Yog-Sothoth is the key and guardian of the gate. Past, present, >future, all > >>are one in Yog-Sothoth. > >>He knows where the Old Ones broke through of old, and where They >shall break > >>through again. > >>_______________________________________________ > >>Histonet mailing list > >>Histonet@lists.utsouthwestern.edu > >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >..................................................................... >: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : >: Sr. Research Specialist University of Arizona : >: (office: AHSC 4212) P.O. Box 245044 : >: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : >: (FAX: 520-626-2097) (email: algranth@u.arizona.edu) >: >:...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From f.finlay <@t> formed.gla.ac.uk Thu Oct 25 08:45:50 2007 From: f.finlay <@t> formed.gla.ac.uk (Finlay Finlay) Date: Thu Oct 25 08:46:00 2007 Subject: [Histonet] Big Chief tablet? In-Reply-To: References: Message-ID: I'm really glad to have found out what a Big Chief tablet is. I'm from Scotland and had no idea but I remember reading about them in "Confederacy of Dunces". Cheers Finlay -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LuAnn Anderson Sent: 24 October 2007 20:13 To: Dana Settembre; Jeanine (CDC/CCID/NCZVED) Bartlett; histonet@lists.utsouthwestern.edu; Andrea Grantham Subject: RE: [Histonet] Big Chief tablet? Yup--still a youngster. lol At 01:55 PM 10/24/2007, Dana Settembre wrote: >I am not a youngster. I am in my 40's and thank you for telling me what >the Big chief tablet is. > > >Dana Settembre, HT ASCP >Immunohistochemistry Lab >UMDNJ - University Hospital >Newark, NJ USA > > > >>> "Bartlett, Jeanine (CDC/CCID/NCZVED)" 10/24/07 > >>> 12:43 >PM >>> >For the youngsters out there---------------- > > The Big Chief tablet was a popular writing notebook for several >generations of young children in the United States. It featured widely >spaced lines, easier to write in for those learning to write. Its >prominence, though, was the cover's representation of a native American >in full headdress, hence "Big Chief." > > > >Jeanine Bartlett >Infectious Diseases Pathology Branch >(404) 639-3590 >jeanine.bartlett@cdc.hhs.gov > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dana >Settembre >Sent: Wednesday, October 24, 2007 12:13 PM >To: histonet@lists.utsouthwestern.edu; Andrea Grantham >Subject: Re: [Histonet] Big Chief tablet? > >What's a "Big Chief tablet"? > > >Dana Settembre, HT ASCP >Immunohistochemistry Lab >UMDNJ - University Hospital >Newark, NJ USA > > > >>> Andrea Grantham 10/24/07 10:12 AM >>> >I had to testify in court once for a murder trial about the lab's chain >of custody protocol. Yes, I was nervous but it was very interesting. >And, I guess the manner in which we received medical-legal specimens >and stored them in a locked cabinet satisfied the lawyers and the jury. >The >victim came to the ER barely alive and the bullets were removed and >received in the lab under the name of John Doe and then he died just as >he was being identified. So later it was questioned how we knew that >the bullets that I had in the lab actually came from the dead person >formerly known as John Doe. That was easy and I really didn't have to >explain anything about histology. >I thought it was interesting that the defense lawyer took notes on a >Big Chief tablet! (Younger people, you probably don't know what this >is). > >Andi > > > >At 03:57 PM 10/23/2007, Joe Nocito wrote: > >Emily, > >I always feel nervous every time I give my opinion. I never had the > >opportunity to testify in court, so I couldn't give you an answer. I > >know, I'm a big help. > > > >Joe > >----- Original Message ----- From: "Emily Sours" > > >To: > >Sent: Tuesday, October 23, 2007 8:31 AM > >Subject: [Histonet] testifying in court? > > > > > >>I would like an honest opinion of the histologists, DNA >technologists, etc > >>(those names can appear in court trials, or those connected with >you)--do > >>you feel nervous offering your opinion in trial to someone's >sentence? I ask > >>because this is the ONLY reason I've never applied for forensic >science > >>positions---I adore the field, and am possibly beneficial to a >PCR/DNA > >>investigation. > >> > >>Emily > >>-- > >>Yog-Sothoth knows the gate. > >>Yog-Sothoth is the gate. > >>Yog-Sothoth is the key and guardian of the gate. Past, present, >future, all > >>are one in Yog-Sothoth. > >>He knows where the Old Ones broke through of old, and where They >shall break > >>through again. > >>_______________________________________________ > >>Histonet mailing list > >>Histonet@lists.utsouthwestern.edu > >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >..................................................................... >: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : >: Sr. Research Specialist University of Arizona : >: (office: AHSC 4212) P.O. Box 245044 : >: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : >: (FAX: 520-626-2097) (email: algranth@u.arizona.edu) >: >:...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________ Disclaimer: This e-mail is intended for the recipient only. If you are not the intended recipient you must not use, disclose, distribute, copy, print, or rely upon this e-mail. If an addressing or transmission error has misdirected this e-mail, please notify the author by replying to this e-mail and delete any local copy from your machine. E-mails are not necessarily secure. The Department of Forensic Medicine & Science (FMS) does not accept responsibility for changes made to this message after it was sent. Opinions, conclusions and other information in this message expressed by others or which do not relate to the official business of FMS shall be understood as neither given nor endorsed by FMS. It is expressly declared that this e-mail does not constitute nor form part of any contract or unilateral obligation unless the contrary is specifically stated in this e-mail and authorised by FMS. From erweber <@t> maxhealth.com Thu Oct 25 08:52:54 2007 From: erweber <@t> maxhealth.com (Eric Weber) Date: Thu Oct 25 08:52:56 2007 Subject: [Histonet] Big Chief tablet? In-Reply-To: <005e01c8170c$87356910$3402a8c0@plab.local> Message-ID: <9D12D4EF30176D4F839EB47F3D0E8436027B86A0@exbk2.maxhealth.com> This is your chance to buy a piece of history. Five Big Chief's Tablets available, current bid is $10. http://cgi.ebay.com/5-Old-Big-Chief-Tablets_W0QQitemZ120174958313QQcmdZV iewItem Eric Weber (866) 466-2974 erweber@maxhealth.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Thursday, October 25, 2007 9:40 AM To: 'Bartlett, Jeanine (CDC/CCID/NCZVED)'; 'Dana Settembre'; histonet@lists.utsouthwestern.edu; 'Andrea Grantham' Subject: RE: [Histonet] Big Chief tablet? I loved Big Cheif! When my daughter was young (she is 19 now) I looked for the Big Chief tablet so I could teach her to write her numbers/letters before she started school but I never did find it. Cheri Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Wednesday, October 24, 2007 11:43 AM To: Dana Settembre; histonet@lists.utsouthwestern.edu; Andrea Grantham Subject: RE: [Histonet] Big Chief tablet? For the youngsters out there---------------- The Big Chief tablet was a popular writing notebook for several generations of young children in the United States. It featured widely spaced lines, easier to write in for those learning to write. Its prominence, though, was the cover's representation of a native American in full headdress, hence "Big Chief." Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dana Settembre Sent: Wednesday, October 24, 2007 12:13 PM To: histonet@lists.utsouthwestern.edu; Andrea Grantham Subject: Re: [Histonet] Big Chief tablet? What's a "Big Chief tablet"? Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Andrea Grantham 10/24/07 10:12 AM >>> I had to testify in court once for a murder trial about the lab's chain of custody protocol. Yes, I was nervous but it was very interesting. And, I guess the manner in which we received medical-legal specimens and stored them in a locked cabinet satisfied the lawyers and the jury. The victim came to the ER barely alive and the bullets were removed and received in the lab under the name of John Doe and then he died just as he was being identified. So later it was questioned how we knew that the bullets that I had in the lab actually came from the dead person formerly known as John Doe. That was easy and I really didn't have to explain anything about histology. I thought it was interesting that the defense lawyer took notes on a Big Chief tablet! (Younger people, you probably don't know what this is). Andi At 03:57 PM 10/23/2007, Joe Nocito wrote: >Emily, >I always feel nervous every time I give my opinion. I never had the >opportunity to testify in court, so I couldn't give you an answer. I >know, I'm a big help. > >Joe >----- Original Message ----- From: "Emily Sours" >To: >Sent: Tuesday, October 23, 2007 8:31 AM >Subject: [Histonet] testifying in court? > > >>I would like an honest opinion of the histologists, DNA technologists, etc >>(those names can appear in court trials, or those connected with you)--do >>you feel nervous offering your opinion in trial to someone's sentence? I ask >>because this is the ONLY reason I've never applied for forensic science >>positions---I adore the field, and am possibly beneficial to a PCR/DNA >>investigation. >> >>Emily >>-- >>Yog-Sothoth knows the gate. >>Yog-Sothoth is the gate. >>Yog-Sothoth is the key and guardian of the gate. Past, present, future, all >>are one in Yog-Sothoth. >>He knows where the Old Ones broke through of old, and where They shall break >>through again. >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Thu Oct 25 08:55:20 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Thu Oct 25 08:56:36 2007 Subject: [Histonet] Big Chief tablet? In-Reply-To: <005f01c8170c$dd6c0730$3402a8c0@plab.local> References: <005f01c8170c$dd6c0730$3402a8c0@plab.local> Message-ID: and Big Chef? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: 25 October 2007 14:42 To: 'LuAnn Anderson'; 'Dana Settembre'; 'Jeanine (CDC/CCID/NCZVED) Bartlett'; histonet@lists.utsouthwestern.edu; 'Andrea Grantham' Subject: RE: [Histonet] Big Chief tablet? I'm 47 and I remember Big Cheif Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LuAnn Anderson Sent: Wednesday, October 24, 2007 2:13 PM To: Dana Settembre; Jeanine (CDC/CCID/NCZVED) Bartlett; histonet@lists.utsouthwestern.edu; Andrea Grantham Subject: RE: [Histonet] Big Chief tablet? Yup--still a youngster. lol At 01:55 PM 10/24/2007, Dana Settembre wrote: >I am not a youngster. I am in my 40's and thank you for telling me what >the Big chief tablet is. > > >Dana Settembre, HT ASCP >Immunohistochemistry Lab >UMDNJ - University Hospital >Newark, NJ USA > > > >>> "Bartlett, Jeanine (CDC/CCID/NCZVED)" 10/24/07 > >>> 12:43 >PM >>> >For the youngsters out there---------------- > > The Big Chief tablet was a popular writing notebook for several >generations of young children in the United States. It featured widely >spaced lines, easier to write in for those learning to write. Its >prominence, though, was the cover's representation of a native American >in full headdress, hence "Big Chief." > > > >Jeanine Bartlett >Infectious Diseases Pathology Branch >(404) 639-3590 >jeanine.bartlett@cdc.hhs.gov > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dana >Settembre >Sent: Wednesday, October 24, 2007 12:13 PM >To: histonet@lists.utsouthwestern.edu; Andrea Grantham >Subject: Re: [Histonet] Big Chief tablet? > >What's a "Big Chief tablet"? > > >Dana Settembre, HT ASCP >Immunohistochemistry Lab >UMDNJ - University Hospital >Newark, NJ USA > > > >>> Andrea Grantham 10/24/07 10:12 AM >>> >I had to testify in court once for a murder trial about the lab's chain >of custody protocol. Yes, I was nervous but it was very interesting. >And, I guess the manner in which we received medical-legal specimens >and stored them in a locked cabinet satisfied the lawyers and the jury. >The >victim came to the ER barely alive and the bullets were removed and >received in the lab under the name of John Doe and then he died just as >he was being identified. So later it was questioned how we knew that >the bullets that I had in the lab actually came from the dead person >formerly known as John Doe. That was easy and I really didn't have to >explain anything about histology. >I thought it was interesting that the defense lawyer took notes on a >Big Chief tablet! (Younger people, you probably don't know what this >is). > >Andi > > > >At 03:57 PM 10/23/2007, Joe Nocito wrote: > >Emily, > >I always feel nervous every time I give my opinion. I never had the > >opportunity to testify in court, so I couldn't give you an answer. I > >know, I'm a big help. > > > >Joe > >----- Original Message ----- From: "Emily Sours" > > >To: > >Sent: Tuesday, October 23, 2007 8:31 AM > >Subject: [Histonet] testifying in court? > > > > > >>I would like an honest opinion of the histologists, DNA >technologists, etc > >>(those names can appear in court trials, or those connected with >you)--do > >>you feel nervous offering your opinion in trial to someone's >sentence? I ask > >>because this is the ONLY reason I've never applied for forensic >science > >>positions---I adore the field, and am possibly beneficial to a >PCR/DNA > >>investigation. > >> > >>Emily > >>-- > >>Yog-Sothoth knows the gate. > >>Yog-Sothoth is the gate. > >>Yog-Sothoth is the key and guardian of the gate. Past, present, >future, all > >>are one in Yog-Sothoth. > >>He knows where the Old Ones broke through of old, and where They >shall break > >>through again. > >>_______________________________________________ > >>Histonet mailing list > >>Histonet@lists.utsouthwestern.edu > >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >..................................................................... >: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : >: Sr. Research Specialist University of Arizona : >: (office: AHSC 4212) P.O. Box 245044 : >: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : >: (FAX: 520-626-2097) (email: algranth@u.arizona.edu) >: >:...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Thu Oct 25 09:07:12 2007 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Thu Oct 25 09:07:30 2007 Subject: [Histonet] Re: Big Chief tablet? Message-ID: Is there ANYTHING that isn't in Wikipedia these days? The Big Chief tablet was a popular writing notebook for several generations of young children in the United States. It featured widely spaced lines, easier to write in for those learning to write. Its prominence, though, was the cover's representation of a native American in full headdress, hence "Big Chief." - The copyright for the Big Chief tablet originally went to the Western Tablet Company of Saint Joseph, Missouri but was sold to the Mead Corp., a leading stationery manufacturer. In January 2001, Everett Pad and Paper purchased Springfield Tablet, the latest manufacturer of Big Chief. They closed operations of their plant, after 80 years of being open, and Big Chief is currently no longer being produced. http://en.wikipedia.org/wiki/Big_Chief_tablet or on eBay? http://cgi.ebay.com/5-Old-Big-Chief-Tablets_W0QQitemZ120174958313QQcmdZViewItem This auction will show you pictures of five of them. Bob Richmond Samurai Pathologist Knoxville TN From mpence <@t> grhs.net Thu Oct 25 09:31:24 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Oct 25 09:31:36 2007 Subject: [Histonet] curious about soaking paraffin blocks. In-Reply-To: <005d01c8170b$a6fb5f30$3402a8c0@plab.local> Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C780@IS-E2K3.grhs.net> So if you place the block on the ice tissue up, what are you soaking, paraffin? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Thursday, October 25, 2007 8:34 AM To: 'Smith Wanda'; 'Anna K. Schultz'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] curious about soaking paraffin blocks. My experience shows soaking sometimes facilitates better sectioning it just depends on the tissue. When I do soak blocks, the small biopsies I place on the ice tissue side up so I don't risk swelling. Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith Wanda Sent: Wednesday, October 24, 2007 10:52 AM To: Anna K. Schultz; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] curious about soaking paraffin blocks. Anna, The danger in leaving faced blocks on the ice too long is it causes the tissue to swell. The part of the tissue that swells will be cut away when you start to cut your sections. This can be bad for tiny biopsy specimens or needle core biopsies, because you may cut the tissue away. I hope this helps! Wanda Wanda G. Smith, HTL(ASCP)HT Trident Medical Center Pathology Supervisor ph: 843-847-4586 fx: 843-847-4296 email: wanda.smith@hcahealthcare.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anna K. Schultz Sent: Wednesday, October 24, 2007 11:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] curious about soaking paraffin blocks. Greetings All, I remember reading on here/archives that paraffin blocks should not be soaked or kept on ice for too long--what is the reasoning behind this? I am just plain curious and still fairly new to the field. I often leave blocks on for a long time and this seems to help or at least not hurt. Thanks, Anna -- Anna K. Schultz SDRC Core A Research Technician Department of Dermatology College of Physicians and Surgeons Columbia University Vanderbilt Clinic 15-211 630 West 168^th Street New York, NY 10032 Phone: (212) 305-4954 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Thu Oct 25 09:40:56 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Oct 25 09:41:05 2007 Subject: [Histonet] curious about soaking paraffin blocks. Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222F02F@wahtntex2.waht.swest.nhs.uk> "So if you place the block on the ice tissue up, what are you soaking, paraffin?" Well if soak it in ice tissue side down what are you cutting frozen sections? Don't understand why you'd want to potentially stick water back into something you've dehydrated; makes no sense. Why not just put blocks face down on a chiller or one of those Peltier effect thingies. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Biology is the least of what makes someone a mother. --Oprah Winfrey This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From gagnone <@t> KGH.KARI.NET Thu Oct 25 09:49:34 2007 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Thu Oct 25 09:49:41 2007 Subject: [Histonet] Breast Lumpectomies..MACRO sections..and prostates Message-ID: Robert, we're not currently doing lumpectomies, but we have recently done 32 whole prostate glands and counting, so here's what we're doing in that regard, as some of the equipment and processes could be useful in your situation. We cut on the Leica RM2255 automated microtome. We obtained their Super Mega Cassette Clamp, (part no 140502 38967). This clamp mounts easily onto the microtome, and holds SurgiPath SuperCassettes (Cat No VSP59067B-BX grey in colour). The beauty of this microtome is that the stroke is 10 mm longer than our previous Reichert-Jung microtomes, I believe it is 70 mm vertically. In terms of slides, we had some on hand, from Fisher and other mfgrs, that are 75x38, 75x50, 75x80 mm, with coverslips that are 35x50, 45x50, and 48x65, whatever will hold and cover the tissue, depending on each specimen. The slides go on our automated stainer, held diagonally with other slides to keep them vertical, and coverslipped by hand. In terms of processing for these sections, we use our normal overnight processing cycle as we would for our routine surgical blocks. These sections are well-fixed before processing, cut at 4-5 mm during grossing. Having said that, breast is a whole different ball game than prostate, and section thickness is going to be more critical, and probably more of a challenge to achieve, and you might adopt a processing protocol that has some steps extended, since your specimen won't be as homogeneous as a prostate gland, i.e. fatty areas, solid/fibrous areas. It would be interesting to learn about these diagnostic excisional lumpectomies. Like the prostates, I presume it is to give the pathologist a better picture of what is going on in a larger area than will fit on a standard slide? I should add that I learned a lot about this topic on the Histonet, which helped a lot in developing our method! Hope I have been of help in turn. I'd also be interested in opening this topic up to other organs that are being sectioned elsewhere in a MACRO way. Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada From bonnie.kaliko <@t> roche.com Thu Oct 25 09:58:11 2007 From: bonnie.kaliko <@t> roche.com (Kaliko, Bonnie) Date: Thu Oct 25 09:58:38 2007 Subject: [Histonet] curious about soaking paraffin blocks. In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBF0222F02F@wahtntex2.waht.swest.nhs.uk> References: <86ADE4EB583CE64799A9924684A0FBBF0222F02F@wahtntex2.waht.swest.nhs.uk> Message-ID: Well as far as animal tissue is concerned, ice and water are needed to hydrate the tissue in order to obtain a section. Each tissue responds differently, some needing time on the ice longer then others. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo Rogerson Sent: Thursday, October 25, 2007 10:41 AM To: Mike Pence; Cheri Miller; Smith Wanda; Anna K. Schultz; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] curious about soaking paraffin blocks. "So if you place the block on the ice tissue up, what are you soaking, paraffin?" Well if soak it in ice tissue side down what are you cutting frozen sections? Don't understand why you'd want to potentially stick water back into something you've dehydrated; makes no sense. Why not just put blocks face down on a chiller or one of those Peltier effect thingies. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Biology is the least of what makes someone a mother. --Oprah Winfrey This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From as3323 <@t> columbia.edu Thu Oct 25 10:15:25 2007 From: as3323 <@t> columbia.edu (Anna K. Schultz) Date: Thu Oct 25 10:15:22 2007 Subject: [Histonet] curious about soaking paraffin blocks. In-Reply-To: References: <86ADE4EB583CE64799A9924684A0FBBF0222F02F@wahtntex2.waht.swest.nhs.uk> Message-ID: <4720B30D.1050801@columbia.edu> Thanks a lot for the responses and I am definitely enjoying the 'debate.' Anna Kaliko, Bonnie wrote: > Well as far as animal tissue is concerned, ice and water are needed to > hydrate the tissue in order to obtain a section. Each tissue responds > differently, some needing time on the ice longer then others. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo > Rogerson > Sent: Thursday, October 25, 2007 10:41 AM > To: Mike Pence; Cheri Miller; Smith Wanda; Anna K. Schultz; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] curious about soaking paraffin blocks. > > "So if you place the block on the ice tissue up, what are you soaking, > paraffin?" > > Well if soak it in ice tissue side down what are you cutting frozen > sections? Don't understand why you'd want to potentially stick water > back into something you've dehydrated; makes no sense. Why not just put > blocks face down on a chiller or one of those Peltier effect thingies. > > > Kemlo Rogerson > Pathology Manager > DD 01934 647057 or extension 3311 > Mob 07749 754194; Pager 07659 597107; > > Biology is the least of what makes someone a mother. --Oprah Winfrey > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. > Please inform me that this message has gone astray before deleting it. > Thank you for your co-operation > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anna K. Schultz SDRC Core A Research Technician Department of Dermatology College of Physicians and Surgeons Columbia University Vanderbilt Clinic 15-211 630 West 168^th Street New York, NY 10032 Phone: (212) 305-4954 From Maxim_71 <@t> mail.ru Thu Oct 25 09:56:27 2007 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Thu Oct 25 10:17:01 2007 Subject: [Histonet] Mouse Skin Processing Message-ID: <1662553864.20071025185627@mail.ru> Igor Deyneko wrote: > I have been havins some problems with processing > mouse skin. The blacks are difficult to section, > then tissue starts flowing away from the > paraffin and the sections do not retain > Hematoxylin stain. That suggest to me that the > processing and paraffin infiltration were poor. > Here's the processing protocol I used for pieces > no larger than 1cm^2. > 70 % Alcohol - 1.5 hr > 70 % Alcohol - 1.5 hr > 70 % Alcohol - 1.0 hr > 80 % Alcohol - 1.0 hr > 95 % Alcohol - 1.0 hr > 95 % Alcohol - 1.0 hr > 100 % Alcohol - 1.5 hr > 100 % Alcohol - 1.5 hr( Due to machine only > having 2 stations for that alcohol) > Xylene - 1.5 hr > Xylene - 1.5 hr(Due to machine having only 2 > stations for Xylene) > Paraffin - 1.0 hr > Paraffin - 1.0 hr > Paraffin - 1.0 hr Igor: We don't have mouse tissues, but for hard human tissues (bone, uterus, fibrous samples, skin etc) have had a similar problem, until start processing with isopropanol and mineral oil. We doing processing manually and now forgot any problems with processing. All these tissues contain too small volumes of water and dehydratation for they must be shortened as mentioned above. Moreover, tissues in isopropanol will not harden as in ethanol. Mineral oil is also very soft clearant than xylene and others aliphatic hydrocarbons. If you want try this method, please let me know. I think that it will be work great for your tissues. Maxim Peshkov, Russia, Taganrog. From Xilong.Li <@t> UTSouthwestern.edu Thu Oct 25 10:30:21 2007 From: Xilong.Li <@t> UTSouthwestern.edu (Xilong Li) Date: Thu Oct 25 10:30:38 2007 Subject: [Histonet] Sirius Red Stain Message-ID: <4720703D020000F00001C818@swnw124.swmed.edu> Hi, All, I try to stain frozen muscle section by Sirius red stain, the results were always not consistent. Background of muscle fiber can not be washed clear to be yellow as expected, it was dark yellow, light yellow or even green, various background color appear in different group staining or different type of muscle stain. In addition, most of protocol in reference or online information require to stain muscle in Sirius stain solution for 1 hour, then wash in acetic solution for a while, then alcohol, xylene. I followed those protocol and found it was hard to wash muscle fiber to yellow even if I washed in acetic solution for 1 hour or increased the concentration of acetic acid solution to 0.5%. Finally I stain muscle in Sirius solution just for 2 minutes, then followed the protocol, some time I can get good stain, some time I can't, it were inconsistent. I just wonder what was the problem of my protocol or staining. I attached three images. Please help me. Any comments would be appreciated. My protocol is as following: Sirius Red Stain Picro-sirius red Sirius Red F3B (CI 35780) Aldrich 36,554-8 synonym Direct Red 80 0.5gm Saturated aqueous Picric Acid 500ml [add a small amount of solid picric acid to ensure saturation: 1.2~1.3% (W/V)] Expiration date minimum 3 years Acidified Water Acetic Acid (glacial) 5 ml Water (DI or tap) 1 liter ? Fix frozen sections 30 minutes in Neutral Buffered Formalin(For paraffin sections deparaffinize and bring slides to water) ? Wash sections in 3 changes of water ? Drain sections well and place into Picro-Sirius solution 2 min ? Wash sections in 2 changes of acidified water(5min) ? Dehydrate in 3 changes of 100% ethanol (5min) ? Clear in xylene and mount in permanent mounting media (5min). Dr. Xilong Li Hypertension Division, Internal Medicine University of Texas Southwestern Medical Center 5323 Harry Hiness Blvd-J4.142 Dallas, TX 75390 Tel: 214-648-9966(L) Fax: 214-648-7902 From Xilong.Li <@t> UTSouthwestern.edu Thu Oct 25 10:31:02 2007 From: Xilong.Li <@t> UTSouthwestern.edu (Xilong Li) Date: Thu Oct 25 10:31:16 2007 Subject: [Histonet] Sirius Red Stain Message-ID: <47207066020000F00001C81C@swnw124.swmed.edu> Hi, All, I try to stain frozen muscle section by Sirius red stain, the results were always not consistent. Background of muscle fiber can not be washed clear to be yellow as expected, it was dark yellow, light yellow or even green, various background color appear in different group staining or different type of muscle stain. In addition, most of protocol in reference or online information require to stain muscle in Sirius stain solution for 1 hour, then wash in acetic solution for a while, then alcohol, xylene. I followed those protocol and found it was hard to wash muscle fiber to yellow even if I washed in acetic solution for 1 hour or increased the concentration of acetic acid solution to 0.5%. Finally I stain muscle in Sirius solution just for 2 minutes, then followed the protocol, some time I can get good stain, some time I can't, it were inconsistent. I just wonder what was the problem of my protocol or staining. I attached three images. Please help me. Any comments would be appreciated. My protocol is as following: Sirius Red Stain Picro-sirius red Sirius Red F3B (CI 35780) Aldrich 36,554-8 synonym Direct Red 80 0.5gm Saturated aqueous Picric Acid 500ml [add a small amount of solid picric acid to ensure saturation: 1.2~1.3% (W/V)] Expiration date minimum 3 years Acidified Water Acetic Acid (glacial) 5 ml Water (DI or tap) 1 liter Fix frozen sections 30 minutes in Neutral Buffered Formalin(For paraffin sections deparaffinize and bring slides to water) Wash sections in 3 changes of water Drain sections well and place into Picro-Sirius solution 2 min Wash sections in 2 changes of acidified water(5min) Dehydrate in 3 changes of 100% ethanol (5min) Clear in xylene and mount in permanent mounting media (5min). Dr. Xilong Li Hypertension Division, Internal Medicine University of Texas Southwestern Medical Center 5323 Harry Hiness Blvd-J4.142 Dallas, TX 75390 Tel: 214-648-9966(L) Fax: 214-648-7902 From jmahoney <@t> alegent.org Thu Oct 25 10:31:36 2007 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Thu Oct 25 10:33:17 2007 Subject: [Histonet] Big Chief tablet? In-Reply-To: References: Message-ID: <472070880200003C0001EC5B@gwia.alegent.org> Wow, another "Flash from the Past"! That was a great read! See you all in Denver. Jan Mahoney Omaha, NE >>> "Finlay Finlay" 10/25/2007 8:45 AM >>> I'm really glad to have found out what a Big Chief tablet is. I'm from Scotland and had no idea but I remember reading about them in "Confederacy of Dunces". Cheers Finlay From Karen.Heckford <@t> CHW.edu Thu Oct 25 10:48:51 2007 From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF) Date: Thu Oct 25 10:49:00 2007 Subject: [Histonet] curious about soaking paraffin blocks. Message-ID: <2842DC75AE43AA4B92954CFB31781BC1821582@CHW-MSG-301.chw.edu> I always face my blocks and then place them on the ice facing upwards. If I need to soak any blocks I use ammonia water only for about a minute. I have in the past used Downey fabric softener (the original scent only) this works great on big tissue like uterus. It also makes the lab smell like a laundry facility. Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathology Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Thursday, October 25, 2007 6:34 AM To: 'Smith Wanda'; 'Anna K. Schultz'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] curious about soaking paraffin blocks. My experience shows soaking sometimes facilitates better sectioning it just depends on the tissue. When I do soak blocks, the small biopsies I place on the ice tissue side up so I don't risk swelling. Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith Wanda Sent: Wednesday, October 24, 2007 10:52 AM To: Anna K. Schultz; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] curious about soaking paraffin blocks. Anna, The danger in leaving faced blocks on the ice too long is it causes the tissue to swell. The part of the tissue that swells will be cut away when you start to cut your sections. This can be bad for tiny biopsy specimens or needle core biopsies, because you may cut the tissue away. I hope this helps! Wanda Wanda G. Smith, HTL(ASCP)HT Trident Medical Center Pathology Supervisor ph: 843-847-4586 fx: 843-847-4296 email: wanda.smith@hcahealthcare.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anna K. Schultz Sent: Wednesday, October 24, 2007 11:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] curious about soaking paraffin blocks. Greetings All, I remember reading on here/archives that paraffin blocks should not be soaked or kept on ice for too long--what is the reasoning behind this? I am just plain curious and still fairly new to the field. I often leave blocks on for a long time and this seems to help or at least not hurt. Thanks, Anna -- Anna K. Schultz SDRC Core A Research Technician Department of Dermatology College of Physicians and Surgeons Columbia University Vanderbilt Clinic 15-211 630 West 168^th Street New York, NY 10032 Phone: (212) 305-4954 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tim.morken <@t> thermofisher.com Thu Oct 25 10:58:23 2007 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Thu Oct 25 11:02:12 2007 Subject: [Histonet] Rodent lymphoid antibody site Message-ID: <6BFF6D137DF6BC43B33891BA96E83B19D9123F@PGHCR-EXMB-VS-1.na.fshrnet.com> Here is a good site for those who need rodent antibodies to rodent lymphoid tissue http://tinyurl.com/ywjsur Tim Morken From b-frederick <@t> northwestern.edu Thu Oct 25 11:34:43 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Oct 25 11:34:55 2007 Subject: [Histonet] Breast Lumpectomies..MACRO sections..and prostates In-Reply-To: Message-ID: <000001c81724$f5a59470$d00f7ca5@lurie.northwestern.edu> Eric, Do you have the catalogue numbers for the coverslips and large slides for the megacassette? We had someone bring us in custom cut slides and getting a coverslip on them was a joke. We used regular coverslips (4 of them) Thanks, Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gagnon, Eric Sent: Thursday, October 25, 2007 9:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Breast Lumpectomies..MACRO sections..and prostates Robert, we're not currently doing lumpectomies, but we have recently done 32 whole prostate glands and counting, so here's what we're doing in that regard, as some of the equipment and processes could be useful in your situation. We cut on the Leica RM2255 automated microtome. We obtained their Super Mega Cassette Clamp, (part no 140502 38967). This clamp mounts easily onto the microtome, and holds SurgiPath SuperCassettes (Cat No VSP59067B-BX grey in colour). The beauty of this microtome is that the stroke is 10 mm longer than our previous Reichert-Jung microtomes, I believe it is 70 mm vertically. In terms of slides, we had some on hand, from Fisher and other mfgrs, that are 75x38, 75x50, 75x80 mm, with coverslips that are 35x50, 45x50, and 48x65, whatever will hold and cover the tissue, depending on each specimen. The slides go on our automated stainer, held diagonally with other slides to keep them vertical, and coverslipped by hand. In terms of processing for these sections, we use our normal overnight processing cycle as we would for our routine surgical blocks. These sections are well-fixed before processing, cut at 4-5 mm during grossing. Having said that, breast is a whole different ball game than prostate, and section thickness is going to be more critical, and probably more of a challenge to achieve, and you might adopt a processing protocol that has some steps extended, since your specimen won't be as homogeneous as a prostate gland, i.e. fatty areas, solid/fibrous areas. It would be interesting to learn about these diagnostic excisional lumpectomies. Like the prostates, I presume it is to give the pathologist a better picture of what is going on in a larger area than will fit on a standard slide? I should add that I learned a lot about this topic on the Histonet, which helped a lot in developing our method! Hope I have been of help in turn. I'd also be interested in opening this topic up to other organs that are being sectioned elsewhere in a MACRO way. Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmiller <@t> physlab.com Thu Oct 25 11:38:44 2007 From: cmiller <@t> physlab.com (Cheri Miller) Date: Thu Oct 25 11:38:50 2007 Subject: [Histonet] curious about soaking paraffin blocks. In-Reply-To: <661949901A768E4F9CC16D8AF8F2838CA1C780@IS-E2K3.grhs.net> References: <005d01c8170b$a6fb5f30$3402a8c0@plab.local> <661949901A768E4F9CC16D8AF8F2838CA1C780@IS-E2K3.grhs.net> Message-ID: <001201c81725$8284a8e0$3402a8c0@plab.local> No, I soak the tissue first in warm soapy water( very briefly) then place them tissue side up on the ice. Otherwise what would be the use??? Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Thursday, October 25, 2007 9:31 AM To: Cheri Miller; Smith Wanda; Anna K. Schultz; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] curious about soaking paraffin blocks. So if you place the block on the ice tissue up, what are you soaking, paraffin? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Thursday, October 25, 2007 8:34 AM To: 'Smith Wanda'; 'Anna K. Schultz'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] curious about soaking paraffin blocks. My experience shows soaking sometimes facilitates better sectioning it just depends on the tissue. When I do soak blocks, the small biopsies I place on the ice tissue side up so I don't risk swelling. Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith Wanda Sent: Wednesday, October 24, 2007 10:52 AM To: Anna K. Schultz; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] curious about soaking paraffin blocks. Anna, The danger in leaving faced blocks on the ice too long is it causes the tissue to swell. The part of the tissue that swells will be cut away when you start to cut your sections. This can be bad for tiny biopsy specimens or needle core biopsies, because you may cut the tissue away. I hope this helps! Wanda Wanda G. Smith, HTL(ASCP)HT Trident Medical Center Pathology Supervisor ph: 843-847-4586 fx: 843-847-4296 email: wanda.smith@hcahealthcare.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anna K. Schultz Sent: Wednesday, October 24, 2007 11:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] curious about soaking paraffin blocks. Greetings All, I remember reading on here/archives that paraffin blocks should not be soaked or kept on ice for too long--what is the reasoning behind this? I am just plain curious and still fairly new to the field. I often leave blocks on for a long time and this seems to help or at least not hurt. Thanks, Anna -- Anna K. Schultz SDRC Core A Research Technician Department of Dermatology College of Physicians and Surgeons Columbia University Vanderbilt Clinic 15-211 630 West 168^th Street New York, NY 10032 Phone: (212) 305-4954 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From cmiller <@t> physlab.com Thu Oct 25 11:46:24 2007 From: cmiller <@t> physlab.com (Cheri Miller) Date: Thu Oct 25 11:46:33 2007 Subject: [Histonet] curious about soaking paraffin blocks. In-Reply-To: <2842DC75AE43AA4B92954CFB31781BC1821582@CHW-MSG-301.chw.edu> References: <2842DC75AE43AA4B92954CFB31781BC1821582@CHW-MSG-301.chw.edu> Message-ID: <001601c81726$948ec600$3402a8c0@plab.local> Some on the net just like to try and make you feel foolish. I know of SEVERAL techs (in diff labs, facilities, states etc.) that soak on occasion when they feel it will benefit the cut etc. It's called technique. As we all know, not all is equal in histology..........Cheri Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Thursday, October 25, 2007 10:49 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] curious about soaking paraffin blocks. I always face my blocks and then place them on the ice facing upwards. If I need to soak any blocks I use ammonia water only for about a minute. I have in the past used Downey fabric softener (the original scent only) this works great on big tissue like uterus. It also makes the lab smell like a laundry facility. Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathology Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Thursday, October 25, 2007 6:34 AM To: 'Smith Wanda'; 'Anna K. Schultz'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] curious about soaking paraffin blocks. My experience shows soaking sometimes facilitates better sectioning it just depends on the tissue. When I do soak blocks, the small biopsies I place on the ice tissue side up so I don't risk swelling. Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith Wanda Sent: Wednesday, October 24, 2007 10:52 AM To: Anna K. Schultz; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] curious about soaking paraffin blocks. Anna, The danger in leaving faced blocks on the ice too long is it causes the tissue to swell. The part of the tissue that swells will be cut away when you start to cut your sections. This can be bad for tiny biopsy specimens or needle core biopsies, because you may cut the tissue away. I hope this helps! Wanda Wanda G. Smith, HTL(ASCP)HT Trident Medical Center Pathology Supervisor ph: 843-847-4586 fx: 843-847-4296 email: wanda.smith@hcahealthcare.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anna K. Schultz Sent: Wednesday, October 24, 2007 11:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] curious about soaking paraffin blocks. Greetings All, I remember reading on here/archives that paraffin blocks should not be soaked or kept on ice for too long--what is the reasoning behind this? I am just plain curious and still fairly new to the field. I often leave blocks on for a long time and this seems to help or at least not hurt. Thanks, Anna -- Anna K. Schultz SDRC Core A Research Technician Department of Dermatology College of Physicians and Surgeons Columbia University Vanderbilt Clinic 15-211 630 West 168^th Street New York, NY 10032 Phone: (212) 305-4954 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From rgarhart <@t> system1.net Thu Oct 25 11:52:25 2007 From: rgarhart <@t> system1.net (Robert Garhart) Date: Thu Oct 25 11:52:17 2007 Subject: [Histonet] AP Lab Manager Opportunity In-Reply-To: <000201c77d29$5ef145f0$800aa8c0@domain.local> References: <000201c77d29$5ef145f0$800aa8c0@domain.local> Message-ID: GREAT AP MANGER POSITION IN THE MIDWEST This is a FT AP Lab Manager position that requires experience in making various entities work together smoothly. Must have excellent communication skills. Will have two direct reports and a total of about 60 staff between histology and cytology. This is located in the Mid West and will consist of a competitive salary as well as a relocation package. Call to discuss your qualifications and potential interest. Robert Garhart Executive Recruiter System 1 Search 678-342-9029 Office rgarhart@system1.net Website: www.system1.net From jkiernan <@t> uwo.ca Thu Oct 25 12:15:33 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Thu Oct 25 12:15:38 2007 Subject: [Histonet] Sirius Red Stain In-Reply-To: <4720703D020000F00001C818@swnw124.swmed.edu> References: <4720703D020000F00001C818@swnw124.swmed.edu> Message-ID: If the frozen sections are of unfixed tissue, immersion in formaldehyde for 30 min will not fix them. The picric acid in the stain will, by a different mechanism, and that's not what's wanted. I suggest fixing the sections by immersing the slides in 95% alcohol for a minute or two. Take back to water and do the staining method as prescribed. This will give you something similar to hydrated paraffin sections. The acetic acid in the post-stain rinse is to prevent loss of bound dyes, not to differentiate the stain. If you want to remove some bound dye (which should never be necessary with this method), use a neutral liquid such as tap water. With muscle you should see red endomysial collagen surrounding yellow (or unstained) individual muscle fibres. The yellow colour from the picric acid is variable; it gets extracted into the alcohols used for dehydration after staining. If you prefer to have colourless cytoplasm, simply leave the slides for a long time in the first of the three changes of 100% alcohol. You didn't say how thick the frozen sections are. If they are thicker than about 20 um they might need a longer wash, with agitation in acidified water. John Kiernan Anatomy, UWO London, Canada --- ----- Original Message ----- From: Xilong Li Date: Thursday, October 25, 2007 11:31 Subject: [Histonet] Sirius Red Stain To: histonet@lists.utsouthwestern.edu > Hi, All, > > I try to stain frozen muscle section by Sirius red stain, the results > were always not consistent. Background of muscle fiber can not > be washed > clear to be yellow as expected, it was dark yellow, light yellow > or even > green, various background color appear in different group > staining or > different type of muscle stain. In addition, most of protocol in > reference or online information require to stain muscle in > Sirius stain > solution for 1 hour, then wash in acetic solution for a while, then > alcohol, xylene. I followed those protocol and found it was hard > to wash > muscle fiber to yellow even if I washed in acetic solution for 1 > hour or > increased the concentration of acetic acid solution to 0.5%. > Finally I > stain muscle in Sirius solution just for 2 minutes, then > followed the > protocol, some time I can get good stain, some time I can't, it were > inconsistent. I just wonder what was the problem of my protocol or > staining. I attached three images. Please help me. > > Any comments would be appreciated. > > > > My protocol is as following: > > Sirius Red Stain > > > Picro-sirius red > > Sirius Red F3B (CI 35780) Aldrich 36,554-8 synonym Direct Red > 80 0.5gm > Saturated aqueous Picric > Acid 500ml > [add a small amount of solid picric acid to ensure saturation: > 1.2~1.3%(W/V)] > > Expiration date minimum 3 years > > Acidified Water > Acetic Acid > (glacial) 5 ml > Water (DI or > tap) 1 liter > > ? Fix frozen sections 30 minutes in Neutral Buffered Formalin(For > paraffin sections deparaffinize and bring slides to water) > ? Wash sections in 3 changes of water > ? Drain sections well and place into Picro-Sirius solution 2 min > ? Wash sections in 2 changes of acidified water(5min) > ? Dehydrate in 3 changes of 100% ethanol (5min) > ? Clear in xylene and mount in permanent mounting media (5min). > > > Dr. Xilong Li > Hypertension Division, Internal Medicine > University of Texas Southwestern Medical Center > 5323 Harry Hiness Blvd-J4.142 > Dallas, TX 75390 > > Tel: 214-648-9966(L) > Fax: 214-648-7902 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From dmccaig <@t> ckha.on.ca Thu Oct 25 13:02:50 2007 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Thu Oct 25 13:02:59 2007 Subject: [Histonet] background staining after DAB Message-ID: Hi We are using the Biocare IHC protocol and using Fisher superfrost plus slides (due to decloaking). When doing the double stain, there is a lot of background stain from the DAB. We do wash well so that is not the problem. I am wondering if is the charge on the slides. I have checked the pH of all the solutions and they are okay. Any ideas. The stain is readable, but does not look too nice. thanks Diana From cstone88 <@t> verizon.net Thu Oct 25 13:09:01 2007 From: cstone88 <@t> verizon.net (Cynthia Stone) Date: Thu Oct 25 13:10:31 2007 Subject: [Histonet] Please take me of the net. Thanks Message-ID: <000601c81732$1fd89370$0201a8c0@puter> From rjbuesa <@t> yahoo.com Thu Oct 25 13:22:49 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 25 13:22:53 2007 Subject: [Histonet] background staining after DAB In-Reply-To: Message-ID: <686755.94523.qm@web61221.mail.yahoo.com> I would check the antibody dilution, it is probably too low. Ren? J. Diana McCaig wrote: Hi We are using the Biocare IHC protocol and using Fisher superfrost plus slides (due to decloaking). When doing the double stain, there is a lot of background stain from the DAB. We do wash well so that is not the problem. I am wondering if is the charge on the slides. I have checked the pH of all the solutions and they are okay. Any ideas. The stain is readable, but does not look too nice. thanks Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From gagnone <@t> KGH.KARI.NET Thu Oct 25 13:54:52 2007 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Thu Oct 25 13:54:59 2007 Subject: [Histonet] Large Slides (was: Breast Lumpectomies..MACRO sections..and prostates) Message-ID: Hi Bernice, Fisher manufactured all the slides and most of the coverslips we have. As I mentioned, we had them on hand, and haven't had to order them for some time, due to having several boxes of the ones we need. So, all I can suggest is contact your ThermoFisherScientific rep for modern-day equivalents. The exception is the 48x65 mm coverslips, which were obtained from Becton Dickinson Labware, their reorder number was 3335. These we obtained more recently, but it was probably a few years ago. Anything has to be better than applying four coverslips on one slide, as you mentioned. Hope this helps, Eric From mcauliff <@t> umdnj.edu Thu Oct 25 14:34:18 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Oct 25 14:34:40 2007 Subject: [Histonet] alternative IHC marker other than GFAP for In-Reply-To: <003401c81663$69367890$4101a8c0@carlba65530bda> References: <003401c81663$69367890$4101a8c0@carlba65530bda> Message-ID: <4720EFBA.3090603@umdnj.edu> Not all astrocytes are GFAP-positive. I suppose someone was looking for a way to find such cells. Geoff Carl Hobbs wrote: > Why do you want an alternative to GFAP as a marker of Astrocytes? > I would be grateful for your answer. > Thanks > > Carl > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From mcauliff <@t> umdnj.edu Thu Oct 25 14:52:32 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Oct 25 14:52:47 2007 Subject: [Histonet] ERK and nuclear counterstain In-Reply-To: <471F2BC9.1020604@tufts.edu> References: <200710221623.l9MGN3Lo001003@mail-proofpoint-3a.usg.tufts.edu> <471F2BC9.1020604@tufts.edu> Message-ID: <4720F400.8060902@umdnj.edu> 1. Nuclear fast red 2. 1% Bismark Brown in 1% acetic acid (gives pale,golden brown nuclei) 3. Basic fuchsin in 1% acetic acid, pinkish-red nuclei. 4. Scarba red. 5. Ask Vector for a recommendation. Geoff Melissa Mazan wrote: > Hi all, I am doing phospho ERK staining - using the Vector MOM kit on > lung tissue - I want to counterstain so that I can enumerate number of > ERK pos nuclei with respect to other nuclei, but if I use hematoxylin > it obscures the signal - everything just looks dark. Any suggestions > for a nuclear counterstain? Many thanks - Melissa > > histonet-request@lists.utsouthwestern.edu wrote: >> >> -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From sccrshlly <@t> yahoo.com Thu Oct 25 17:58:18 2007 From: sccrshlly <@t> yahoo.com (Shelly Coker) Date: Thu Oct 25 17:58:23 2007 Subject: [Histonet] Tryptase Stain for Mast Cell in Gastrointestinal Specimens Message-ID: <745429.37353.qm@web90303.mail.mud.yahoo.com> Hello everyone, Our pathologist has asked me to inquire about a Tryptase stain for mast cell tumors in gastro specimens. She said is one of those "latest/greatest" things. Does anyone have any experience with this stain? Thanks, Shelly __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From jnocito <@t> satx.rr.com Thu Oct 25 18:06:26 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Oct 25 18:06:28 2007 Subject: [Histonet] Re: Big Chief tablet? References: Message-ID: <006d01c8175b$ab9d88b0$0302a8c0@yourxhtr8hvc4p> yeah, Joe the Toe. Am I being selfish, self-centered and obnoxious? Oh yeah, I'm always obnoxious. ----- Original Message ----- From: "Robert Richmond" To: Sent: Thursday, October 25, 2007 9:07 AM Subject: [Histonet] Re: Big Chief tablet? > Is there ANYTHING that isn't in Wikipedia these days? > > The Big Chief tablet was a popular writing notebook for several > generations of young children in the United States. It featured widely > spaced lines, easier to write in for those learning to write. Its > prominence, though, was the cover's representation of a native > American in full headdress, hence "Big Chief." - The copyright for the > Big Chief tablet originally went to the Western Tablet Company of > Saint Joseph, Missouri but was sold to the Mead Corp., a leading > stationery manufacturer. In January 2001, Everett Pad and Paper > purchased Springfield Tablet, the latest manufacturer of Big Chief. > They closed operations of their plant, after 80 years of being open, > and Big Chief is currently no longer being produced. > > http://en.wikipedia.org/wiki/Big_Chief_tablet > > or on eBay? > > http://cgi.ebay.com/5-Old-Big-Chief-Tablets_W0QQitemZ120174958313QQcmdZViewItem > > This auction will show you pictures of five of them. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Thu Oct 25 18:06:26 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Oct 25 18:08:06 2007 Subject: [Histonet] Re: Big Chief tablet? References: Message-ID: <009d01c8175b$e6aded00$0302a8c0@yourxhtr8hvc4p> yeah, Joe the Toe. Am I being selfish, self-centered and obnoxious? Oh yeah, I'm always obnoxious. ----- Original Message ----- From: "Robert Richmond" To: Sent: Thursday, October 25, 2007 9:07 AM Subject: [Histonet] Re: Big Chief tablet? > Is there ANYTHING that isn't in Wikipedia these days? > > The Big Chief tablet was a popular writing notebook for several > generations of young children in the United States. It featured widely > spaced lines, easier to write in for those learning to write. Its > prominence, though, was the cover's representation of a native > American in full headdress, hence "Big Chief." - The copyright for the > Big Chief tablet originally went to the Western Tablet Company of > Saint Joseph, Missouri but was sold to the Mead Corp., a leading > stationery manufacturer. In January 2001, Everett Pad and Paper > purchased Springfield Tablet, the latest manufacturer of Big Chief. > They closed operations of their plant, after 80 years of being open, > and Big Chief is currently no longer being produced. > > http://en.wikipedia.org/wiki/Big_Chief_tablet > > or on eBay? > > http://cgi.ebay.com/5-Old-Big-Chief-Tablets_W0QQitemZ120174958313QQcmdZViewItem > > This auction will show you pictures of five of them. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RichmanL <@t> MedImmune.com Thu Oct 25 18:17:18 2007 From: RichmanL <@t> MedImmune.com (Richman, Laura) Date: Thu Oct 25 18:17:34 2007 Subject: [Histonet] Pathology Associate/Immunohistochemistry Technician position at MedImmune, Inc. In-Reply-To: References: Message-ID: <5B7214BEC09FBC49B30DB9D2345554EA01630B68@MD1EV002.medimmune.com> MedImmune, Inc. is expanding its Experimental Pathology unit in Gaithersburg, MD and is seeking a highly motivated Pathology Associate to join our interactive, fast-paced group. The successful candidate will be responsible for the following: Major Duties and Responsibilities: * Responsible for the proper and effective conduct of technically demanding immunohistochemical and tissue/cell-based experiments (including tissue cross-reactivity studies), with oversight and support from immediate supervisor. * Makes detailed observations, analyzes data, interprets results, with potential for presenting findings at internal meetings. * Exercises interest and potential for significant technical discretion in design, execution and interpretation of experimental outcomes. * Conducts research and development in collaboration with supervisor and other individuals within Research department * Prepares precise technical reports, summaries, protocols, quantitative analyses, and maintains appropriate documentation of experiments in laboratory notebooks, with oversight and guidance from supervisor. May contribute to preparation of manuscripts and/or patent applications. * Responsible for the correct operation of utilized lab equipment. * Instructs and assists others with the proper and effective conduct of lab procedures when appropriate. * Requires minimal instructions on routine work, general instructions on new assignments. Can follow protocols without supervision. Reduces procedures to practice. * Works to develop a broader skill base, competence with lab techniques, procedures and protocols. Requirements/Qualifications: * Education: Requires a Bachelor's/Master's degree in a scientific discipline or equivalent with a minimum of 2 years experience with a Bachelor's (2-4 years) or 1-2 years with a Master's and demonstrated working knowledge of scientific principles. * Experience: Minimum of 2 years experience with a Bachelor's (2-4 years) or 1-2 years with a Master's Degree. * Special Skills/Abilities: GLP experience desired but not required. * Job Complexity: moderate * Supervision: moderate level of independence About MedImmune: MedImmune is dedicated to helping patients live better lives through advances in science and medicine. Hundreds of thousands of patients have benefited from our products, which are designed to treat or prevent infectious diseases, cancer and inflammatory diseases. Our extensive research and development efforts are focused on these same areas. Founded in 1988, MedImmune is one of the world's premier biotechnology firms, with more than 2,500 employees worldwide. Continued investment in people and infrastructure is necessary to support our growing portfolio of products and programs, and we are committed to hiring and retaining the best talent. MedImmune employees share a commitment to helping people enjoy longer, healthier and more fulfilling lives. Individual contributions are highly valued in an environment characterized by an open exchange of ideas and information. This entrepreneurial spirit is central to our culture, where a passion for excellence meets cutting-edge technology. Laura K. Richman, DVM, PhD Diplomate, ACVP Director, Pathology MedImmune, Inc. One MedImmune Way Gaithersburg, MD 20878 phone: (301) 398-4741 fax: (301) 398-9741 ****************************************** To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From cfarish <@t> csu.edu.au Thu Oct 25 18:42:16 2007 From: cfarish <@t> csu.edu.au (Farish, Craig) Date: Thu Oct 25 18:42:46 2007 Subject: [Histonet] Breast Lumpectomies..MACRO sections..and Prostates Message-ID: <79A000B60AFE8045BA2581119DFEC44402A2518C@ESWW01.CSUMain.csu.edu.au> Hi Bernice, I'm currently in the process of sourcing plastic supermega cassettes + all the associated paraphernalia. I'm struggling to find a reasonable supplier in Australia so I'm dealing with a company called cellpath in the UK (www.cellpath.co.uk ). Up til now they've been excellent to deal with and can supply cassettes (71x51x16mm), appropriate moulds, slides, coverslips and storage for the whole set-up. Freight costs are however, a real drag (one of the few disadvantages of living down here). Hope this helps, Craig Craig (Joe) Farish Technical Officer School of Agricultural and Veterinary Sciences Charles Sturt University Locked Bag 588 Wagga Wagga NSW 2678 Ph. (02) 6933 2079 Fax. (02) 6933 2812 Email. cfarish@csu.edu.au From talulahgosh <@t> gmail.com Fri Oct 26 01:04:10 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Oct 26 01:04:14 2007 Subject: [Histonet] Re: Big Chief tablet? In-Reply-To: <009d01c8175b$e6aded00$0302a8c0@yourxhtr8hvc4p> References: <009d01c8175b$e6aded00$0302a8c0@yourxhtr8hvc4p> Message-ID: may i ask about "joe the toe"? i bet there isn't a wiki on that. Emily -- When you visualize a man or woman carefully, you could always begin to feel pity...when you saw the lines at the corners of the eyes, the shape of the mouth, how the hair grew, it was impossible to hate. -Graham Greene, The Power and the Glory From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Oct 26 01:54:05 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Oct 26 01:54:13 2007 Subject: [Histonet] curious about soaking paraffin blocks. Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222F037@wahtntex2.waht.swest.nhs.uk> "Well as far as animal tissue is concerned, ice and water are needed to hydrate the tissue in order to obtain a section. Each tissue responds differently, some needing time on the ice longer then others." That statement makes no sense, you rehydrate after sectioning and drying off and prior to staining with aqueous stains; you don't rehydrate prior to sectioning. I can't believe you believe what you appear to have said. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Biology is the least of what makes someone a mother. --Oprah Winfrey This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Oct 26 01:56:41 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Oct 26 01:56:47 2007 Subject: [Histonet] curious about soaking paraffin blocks. Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222F038@wahtntex2.waht.swest.nhs.uk> "I always face my blocks and then place them on the ice facing upwards. If I need to soak any blocks I use ammonia water only for about a minute. I have in the past used Downey fabric softener (the original scent only) this works great on big tissue like uterus. It also makes the lab smell like a laundry facility." Now that makes sense as you are alluding to 'softening' the tissue with fabric softener which you must do from time to time (but surely not regularly) for those very hard blocks. I've always maintained that if you fix tissue properly and process them optimally you won't need these fancy reclamation methods. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Biology is the least of what makes someone a mother. --Oprah Winfrey This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Oct 26 01:58:46 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Oct 26 01:58:52 2007 Subject: [Histonet] curious about soaking paraffin blocks. Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222F039@wahtntex2.waht.swest.nhs.uk> "Some on the net just like to try and make you feel foolish. I know of SEVERAL techs (in diff labs, facilities, states etc.) that soak on occasion when they feel it will benefit the cut etc. It's called technique. As we all know, not all is equal in histology..........Cheri" We are soaking for different reasons Cheri but I'm surprised it seems to be a regular procedure in the US probably used to mask poor fixation or processing. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Biology is the least of what makes someone a mother. --Oprah Winfrey This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From talulahgosh <@t> gmail.com Fri Oct 26 02:22:06 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Oct 26 02:22:15 2007 Subject: [Histonet] cryo-sections, old question? Message-ID: while searching through my discarded emails from histonet for another topic, i found an email with this: "For the last 5 years I have been promoting a system of face down embedding tissue for frozen section which I developed over my 25 year surgical pathology practice. The system is rapid, very precise and wastes very little tissue." what is face down embedding? and what is heat-extracting a block--(the email later mentions you can heat-extract it) couldn't you just cut it off with a razor blade, or do others try to conserve mounting media and make super tiny blocks (about 500 ul plus specimen equals tiny to me)? emily -- When you visualize a man or woman carefully, you could always begin to feel pity...when you saw the lines at the corners of the eyes, the shape of the mouth, how the hair grew, it was impossible to hate. -Graham Greene, The Power and the Glory From Susan.Walzer <@t> HCAHealthcare.com Fri Oct 26 02:27:55 2007 From: Susan.Walzer <@t> HCAHealthcare.com (Walzer Susan) Date: Fri Oct 26 02:28:05 2007 Subject: FW: [Histonet] curious about soaking paraffin blocks. Message-ID: <471953BC63077941B82C26A4338272B42F0543@ORLEV03.hca.corpad.net> As Lee Luna used to say..."Rehydrate, rehydrate, rehydrate" -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo Rogerson Sent: Friday, October 26, 2007 2:59 AM To: Cheri Miller; Heckford, Karen - SMMC-SF; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] curious about soaking paraffin blocks. "Some on the net just like to try and make you feel foolish. I know of SEVERAL techs (in diff labs, facilities, states etc.) that soak on occasion when they feel it will benefit the cut etc. It's called technique. As we all know, not all is equal in histology..........Cheri" We are soaking for different reasons Cheri but I'm surprised it seems to be a regular procedure in the US probably used to mask poor fixation or processing. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Biology is the least of what makes someone a mother. --Oprah Winfrey This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Susan.Walzer <@t> HCAHealthcare.com Fri Oct 26 02:29:53 2007 From: Susan.Walzer <@t> HCAHealthcare.com (Walzer Susan) Date: Fri Oct 26 02:30:00 2007 Subject: FW: [Histonet] curious about soaking paraffin blocks. Message-ID: <471953BC63077941B82C26A4338272B42F0544@ORLEV03.hca.corpad.net> I keep a bottle of soapy water with ammonia to soak blocks. This helps with tough tissue and the ammonia is great for bloody tissue. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Thursday, October 25, 2007 11:49 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] curious about soaking paraffin blocks. I always face my blocks and then place them on the ice facing upwards. If I need to soak any blocks I use ammonia water only for about a minute. I have in the past used Downey fabric softener (the original scent only) this works great on big tissue like uterus. It also makes the lab smell like a laundry facility. Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathology Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Thursday, October 25, 2007 6:34 AM To: 'Smith Wanda'; 'Anna K. Schultz'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] curious about soaking paraffin blocks. My experience shows soaking sometimes facilitates better sectioning it just depends on the tissue. When I do soak blocks, the small biopsies I place on the ice tissue side up so I don't risk swelling. Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith Wanda Sent: Wednesday, October 24, 2007 10:52 AM To: Anna K. Schultz; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] curious about soaking paraffin blocks. Anna, The danger in leaving faced blocks on the ice too long is it causes the tissue to swell. The part of the tissue that swells will be cut away when you start to cut your sections. This can be bad for tiny biopsy specimens or needle core biopsies, because you may cut the tissue away. I hope this helps! Wanda Wanda G. Smith, HTL(ASCP)HT Trident Medical Center Pathology Supervisor ph: 843-847-4586 fx: 843-847-4296 email: wanda.smith@hcahealthcare.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anna K. Schultz Sent: Wednesday, October 24, 2007 11:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] curious about soaking paraffin blocks. Greetings All, I remember reading on here/archives that paraffin blocks should not be soaked or kept on ice for too long--what is the reasoning behind this? I am just plain curious and still fairly new to the field. I often leave blocks on for a long time and this seems to help or at least not hurt. Thanks, Anna -- Anna K. Schultz SDRC Core A Research Technician Department of Dermatology College of Physicians and Surgeons Columbia University Vanderbilt Clinic 15-211 630 West 168^th Street New York, NY 10032 Phone: (212) 305-4954 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pieronelva01 <@t> bigpond.com Fri Oct 26 05:46:02 2007 From: pieronelva01 <@t> bigpond.com (Piero Nelva) Date: Fri Oct 26 05:46:13 2007 Subject: [Histonet] curious about soaking paraffin blocks. References: <2842DC75AE43AA4B92954CFB31781BC1821582@CHW-MSG-301.chw.edu> <001601c81726$948ec600$3402a8c0@plab.local> Message-ID: <002e01c817bd$66ddaa40$8a74be7c@pentium4> I find that soaking blocks on a wet tissue at room temperature, as opposed to an ice water bath is very helpful to stop haemorrhagic tissues shattering. Piero Nelva Anatomical Pathology Monash Medical Centre Melbourne Australia ----- Original Message ----- From: "Cheri Miller" To: "'Heckford, Karen - SMMC-SF'" ; Sent: Friday, October 26, 2007 2:46 AM Subject: RE: [Histonet] curious about soaking paraffin blocks. > Some on the net just like to try and make you feel foolish. I know of > SEVERAL techs (in diff labs, facilities, states etc.) that soak on > occasion > when they feel it will benefit the cut etc. It's called technique. As we > all > know, not all is equal in histology..........Cheri > > Cheri Miller HT ASCP > Histology Supervisor > Physicians Laboratory Services, Inc. > Omaha, NE 68117 > 402 738 5052 > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. > If > you are not the addressee intended / indicated or agent responsible for > delivering it to the addressee, you are hereby notified that you are in > possession of confidential and privileged information. Any dissemination, > distribution, or copying of this e-mail is strictly prohibited. If you > have > received this message in error, please notify the sender immediately and > delete this email from your system. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, > Karen - SMMC-SF > Sent: Thursday, October 25, 2007 10:49 AM > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] curious about soaking paraffin blocks. > > I always face my blocks and then place them on the ice facing upwards. If > I need to soak any blocks I use ammonia water only for about a minute. I > have in the past used Downey fabric softener (the original scent only) > this > works great on big tissue like uterus. It also makes the lab smell like a > laundry facility. > Karen Heckford HT (ASCP) CE > Lead Histology Technician > Histology/Pathology Department > St. Mary's Medical Center > 450 Stanyan St. > San Francisco, Ca. 94117 > 415-668-1000 ext. 6167 > Fax: 415-750-8123 > email: kheckfor@chw.edu > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri > Miller > Sent: Thursday, October 25, 2007 6:34 AM > To: 'Smith Wanda'; 'Anna K. Schultz'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] curious about soaking paraffin blocks. > > My experience shows soaking sometimes facilitates better sectioning it > just > depends on the tissue. When I do soak blocks, the small biopsies I place > on > the ice tissue side up so I don't risk swelling. > Cheri Miller HT ASCP > Histology Supervisor > Physicians Laboratory Services, Inc. > Omaha, NE 68117 > 402 738 5052 > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. > If > you are not the addressee intended / indicated or agent responsible for > delivering it to the addressee, you are hereby notified that you are in > possession of confidential and privileged information. Any dissemination, > distribution, or copying of this e-mail is strictly prohibited. If you > have > received this message in error, please notify the sender immediately and > delete this email from your system. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith > Wanda > Sent: Wednesday, October 24, 2007 10:52 AM > To: Anna K. Schultz; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] curious about soaking paraffin blocks. > > Anna, > The danger in leaving faced blocks on the ice too long is it causes the > tissue to swell. The part of the tissue that swells will be cut away when > you start to cut your sections. This can be bad for tiny biopsy specimens > or needle core biopsies, because you may cut the tissue away. > I hope this helps! > Wanda > > Wanda G. Smith, HTL(ASCP)HT > Trident Medical Center > Pathology Supervisor > ph: 843-847-4586 > fx: 843-847-4296 > email: wanda.smith@hcahealthcare.com > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anna K. > Schultz > Sent: Wednesday, October 24, 2007 11:06 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] curious about soaking paraffin blocks. > > Greetings All, > I remember reading on here/archives that paraffin blocks should not be > > soaked or kept on ice for too long--what is the reasoning behind this? > I am just plain curious and still fairly new to the field. I often leave > blocks on for a long time and this seems to help or at least not hurt. > > Thanks, > Anna > -- > Anna K. Schultz > SDRC Core A Research Technician > Department of Dermatology > College of Physicians and Surgeons > Columbia University > Vanderbilt Clinic 15-211 > 630 West 168^th Street > New York, NY 10032 > Phone: (212) 305-4954 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. > If > you are not the addressee intended / indicated or agent responsible for > delivering it to the addressee, you are hereby notified that you are in > possession of confidential and privileged information. Any dissemination, > distribution, or copying of this e-mail is strictly prohibited. If you > have > received this message in error, please notify the sender immediately and > delete this email from your system. > > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. > If > you are not the addressee intended / indicated or agent responsible for > delivering it to the addressee, you are hereby notified that you are in > possession of confidential and privileged information. Any dissemination, > distribution, or copying of this e-mail is strictly prohibited. If you > have > received this message in error, please notify the sender immediately and > delete this email from your system. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. > If > you are not the addressee intended / indicated or agent responsible for > delivering it to the addressee, you are hereby notified that you are in > possession of confidential and privileged information. Any dissemination, > distribution, or copying of this e-mail is strictly prohibited. If you > have > received this message in error, please notify the sender immediately and > delete this email from your system. > > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. > If you are not the addressee intended / indicated or agent responsible for > delivering it to the addressee, you are hereby notified that you are in > possession of confidential and privileged information. Any dissemination, > distribution, or copying of this e-mail is strictly prohibited. If you > have received this message in error, please notify the sender immediately > and delete this email from your system. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.5.503 / Virus Database: 269.15.11/1093 - Release Date: > 10/25/2007 5:38 PM > > From JWEEMS <@t> sjha.org Fri Oct 26 05:52:44 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Oct 26 05:53:01 2007 Subject: [Histonet] curious about soaking paraffin blocks. In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBF0222F037@wahtntex2.waht.swest.nhs.uk> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F4034@sjhaexc02.sjha.org> I believe overdehydration has something to do with extracting the bound water. I rehydrate - have for years, since we are trying to rush processing without the adequate fixation we used to have by leaving large specimens overnight in formalin. I blow on the blocks, put some of the colon bxs in the water bath for a few seconds, put large specimens on ice - anything to get a good section. The key to the perfect section after soaking is not to leave the block so long that the tissue is damaged. The block then has to be faced to the exact spot that the perfect section can be taken - not where the tissue is still too soaked or after you've moved past that place again. Once again this part has to do with perception and experience. My 2 cents again...j:>) Joyce Weems Saint Joseph's Hospital of Atlanta 404-851-7376 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo Rogerson Sent: Friday, October 26, 2007 2:54 AM To: Kaliko, Bonnie; Mike Pence; Cheri Miller; Smith Wanda; Anna K. Schultz; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] curious about soaking paraffin blocks. "Well as far as animal tissue is concerned, ice and water are needed to hydrate the tissue in order to obtain a section. Each tissue responds differently, some needing time on the ice longer then others." That statement makes no sense, you rehydrate after sectioning and drying off and prior to staining with aqueous stains; you don't rehydrate prior to sectioning. I can't believe you believe what you appear to have said. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Oct 26 06:24:04 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Oct 26 06:24:12 2007 Subject: [Histonet] curious about soaking paraffin blocks. Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222F041@wahtntex2.waht.swest.nhs.uk> "The key to the perfect section after soaking is not to leave the block so long that the tissue is damaged. The block then has to be faced to the exact spot that the perfect section can be taken - not where the tissue is still too soaked or after you've moved past that place again. Once again this part has to do with perception and experience." Helpful.... so obviously a way of answering poor fixation and/ or processing; sorry but I never came across this in the UK. Do any of you Brits do this? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Biology is the least of what makes someone a mother. --Oprah Winfrey This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From asmith <@t> mail.barry.edu Fri Oct 26 07:53:16 2007 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Oct 26 07:53:37 2007 Subject: [Histonet] soaking paraffin blocks. In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBF0222F041@wahtntex2.waht.swest.nhs.uk> Message-ID: <7DBCCC1FBC77C94F99F920D0CA6400B61E4132@exchsrv01.barrynet.barry.edu> Yes, soaking the faced block is a cure for poor processing, but it works. I find that holding a moist ice cube against the block face for about 30 seconds does wonders for a poorly processed block. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo Rogerson Sent: Friday, October 26, 2007 7:24 AM To: Weems, Joyce; Kaliko, Bonnie; Mike Pence; Cheri Miller; Smith Wanda; Anna K. Schultz; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] curious about soaking paraffin blocks. "The key to the perfect section after soaking is not to leave the block so long that the tissue is damaged. The block then has to be faced to the exact spot that the perfect section can be taken - not where the tissue is still too soaked or after you've moved past that place again. Once again this part has to do with perception and experience." Helpful.... so obviously a way of answering poor fixation and/ or processing; sorry but I never came across this in the UK. Do any of you Brits do this? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Biology is the least of what makes someone a mother. --Oprah Winfrey This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From slappycraw <@t> yahoo.com Fri Oct 26 08:08:15 2007 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Fri Oct 26 08:08:20 2007 Subject: [Histonet] Tryptase Stain for Mast Cell in Gastrointestinal Specimens In-Reply-To: <745429.37353.qm@web90303.mail.mud.yahoo.com> Message-ID: <499236.27587.qm@web53605.mail.re2.yahoo.com> We use Abcam ab2378 for human and monkey tissue with anti mouse polymer from dako and it works very well. Shelly Coker wrote: Hello everyone, Our pathologist has asked me to inquire about a Tryptase stain for mast cell tumors in gastro specimens. She said is one of those "latest/greatest" things. Does anyone have any experience with this stain? Thanks, Shelly __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Larry A. Woody Amgen Seattle, Wa. __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From bonnie.kaliko <@t> roche.com Fri Oct 26 08:27:41 2007 From: bonnie.kaliko <@t> roche.com (Kaliko, Bonnie) Date: Fri Oct 26 08:28:21 2007 Subject: [Histonet] curious about soaking paraffin blocks. In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBF0222F041@wahtntex2.waht.swest.nhs.uk> References: <86ADE4EB583CE64799A9924684A0FBBF0222F041@wahtntex2.waht.swest.nhs.uk> Message-ID: Kemlo, you obviously do not work with animal tissue which is VERY dry. It has nothing to do with poor fixation or over dehydration. Liver, bone, spleen, muscle is impossible to cut unless it is placed face down on ice. If you ever try cutting animal tissue and manage to get a good section without soaking it, then you should apply for a patent application. -----Original Message----- From: Kemlo Rogerson [mailto:Kemlo.Rogerson@waht.swest.nhs.uk] Sent: Friday, October 26, 2007 7:24 AM To: Weems, Joyce; Kaliko, Bonnie {Nonc~Nutley}; Mike Pence; Cheri Miller; Smith Wanda; Anna K. Schultz; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] curious about soaking paraffin blocks. "The key to the perfect section after soaking is not to leave the block so long that the tissue is damaged. The block then has to be faced to the exact spot that the perfect section can be taken - not where the tissue is still too soaked or after you've moved past that place again. Once again this part has to do with perception and experience." Helpful.... so obviously a way of answering poor fixation and/ or processing; sorry but I never came across this in the UK. Do any of you Brits do this? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Biology is the least of what makes someone a mother. --Oprah Winfrey This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From llewllew <@t> shaw.ca Fri Oct 26 09:02:56 2007 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Fri Oct 26 09:03:43 2007 Subject: [Histonet] curious about soaking paraffin blocks. References: <86ADE4EB583CE64799A9924684A0FBBF0222F039@wahtntex2.waht.swest.nhs.uk> Message-ID: <000c01c817d8$e9098870$05024246@yourlk4rlmsu> I think that hits the nail squarely on the head. I don't know about the UK anymore, but in North America, including Canada, there is a "fixation" about 24 hour service or less. Since 10% NBF is still the commonest fixative, that must entail inadequate treatment with the consequent alcoholic fixation that ensues during processing and the brittle, hard little bits of tissue that are the final product. Soaking in iced water for a short time does soften these enough to get sections, and has become an integral part of microtomy over here. Bryan Llewellyn ----- Original Message ----- From: "Kemlo Rogerson" To: "Cheri Miller" ; "Heckford, Karen - SMMC-SF" ; Sent: Thursday, October 25, 2007 11:58 PM Subject: RE: [Histonet] curious about soaking paraffin blocks. "Some on the net just like to try and make you feel foolish. I know of SEVERAL techs (in diff labs, facilities, states etc.) that soak on occasion when they feel it will benefit the cut etc. It's called technique. As we all know, not all is equal in histology..........Cheri" We are soaking for different reasons Cheri but I'm surprised it seems to be a regular procedure in the US probably used to mask poor fixation or processing. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Biology is the least of what makes someone a mother. --Oprah Winfrey This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Oct 26 09:16:20 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Oct 26 09:16:29 2007 Subject: [Histonet] curious about soaking paraffin blocks. Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222F046@wahtntex2.waht.swest.nhs.uk> "Kemlo, you obviously do not work with animal tissue which is VERY dry. It has nothing to do with poor fixation or over dehydration. Liver, bone, spleen, muscle is impossible to cut unless it is placed face down on ice. If you ever try cutting animal tissue and manage to get a good section without soaking it, then you should apply for a patent application." I ran a Veterinary Histology Laboratory from a number of years and cut sections from little birdies to big bad apes; never had the problem you allude to. Must be the way we process in the UK; or is it the water . Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Biology is the least of what makes someone a mother. --Oprah Winfrey This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From bonnie.kaliko <@t> roche.com Fri Oct 26 09:19:39 2007 From: bonnie.kaliko <@t> roche.com (Kaliko, Bonnie) Date: Fri Oct 26 09:20:18 2007 Subject: [Histonet] curious about soaking paraffin blocks. In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBF0222F046@wahtntex2.waht.swest.nhs.uk> References: <86ADE4EB583CE64799A9924684A0FBBF0222F046@wahtntex2.waht.swest.nhs.uk> Message-ID: I would love to find out the processing schedule. -----Original Message----- From: Kemlo Rogerson [mailto:Kemlo.Rogerson@waht.swest.nhs.uk] Sent: Friday, October 26, 2007 10:16 AM To: Kaliko, Bonnie {Nonc~Nutley}; Weems, Joyce; Mike Pence; Cheri Miller; Smith Wanda; Anna K. Schultz; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] curious about soaking paraffin blocks. "Kemlo, you obviously do not work with animal tissue which is VERY dry. It has nothing to do with poor fixation or over dehydration. Liver, bone, spleen, muscle is impossible to cut unless it is placed face down on ice. If you ever try cutting animal tissue and manage to get a good section without soaking it, then you should apply for a patent application." I ran a Veterinary Histology Laboratory from a number of years and cut sections from little birdies to big bad apes; never had the problem you allude to. Must be the way we process in the UK; or is it the water . Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Biology is the least of what makes someone a mother. --Oprah Winfrey This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Oct 26 09:24:17 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Oct 26 09:24:24 2007 Subject: [Histonet] curious about soaking paraffin blocks. Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222F047@wahtntex2.waht.swest.nhs.uk> I tended to process until they were processed; some tissue took longer than others the secret was when they looked clear in xylene. If they weren't see through I went back to alcohol; obviously NOT an automatic processing procedure. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Biology is the least of what makes someone a mother. --Oprah Winfrey This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From bonnie.kaliko <@t> roche.com Fri Oct 26 09:34:41 2007 From: bonnie.kaliko <@t> roche.com (Kaliko, Bonnie) Date: Fri Oct 26 09:35:14 2007 Subject: [Histonet] curious about soaking paraffin blocks. In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBF0222F047@wahtntex2.waht.swest.nhs.uk> References: <86ADE4EB583CE64799A9924684A0FBBF0222F047@wahtntex2.waht.swest.nhs.uk> Message-ID: Since we process every organ of rat, mouse, dog, rabbit and monkey on many animals, processing by hand would be archaic. The amount of time each species is processed depends on the tissue size. Mouse having the shortest processing schedule. Monkey is the easiest to section because it is so close to human tissue. All tissue is fully fixed in 10% NBF before processing. -----Original Message----- From: Kemlo Rogerson [mailto:Kemlo.Rogerson@waht.swest.nhs.uk] Sent: Friday, October 26, 2007 10:24 AM To: Kaliko, Bonnie {Nonc~Nutley}; Weems, Joyce; Mike Pence; Cheri Miller; Smith Wanda; Anna K. Schultz; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] curious about soaking paraffin blocks. I tended to process until they were processed; some tissue took longer than others the secret was when they looked clear in xylene. If they weren't see through I went back to alcohol; obviously NOT an automatic processing procedure. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Biology is the least of what makes someone a mother. --Oprah Winfrey This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From sheila_adey <@t> hotmail.com Fri Oct 26 10:16:31 2007 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Fri Oct 26 10:16:39 2007 Subject: [Histonet] background staining after DAB In-Reply-To: <686755.94523.qm@web61221.mail.yahoo.com> References: <686755.94523.qm@web61221.mail.yahoo.com> Message-ID: Hi Rene, Could you explain to me why having a low concentration of antibody would give background staining? I am having trouble only on my negatives with background. (we use AEC). Sheila Adey HT MLTPort Huron HospitalMichigan> Date: Thu, 25 Oct 2007 11:22:49 -0700> From: rjbuesa@yahoo.com> To: dmccaig@ckha.on.ca; histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] background staining after DAB> CC: > > I would check the antibody dilution, it is probably too low.> Ren? J.> > Diana McCaig wrote:> Hi> We are using the Biocare IHC protocol and using Fisher superfrost plus> slides (due to decloaking). When doing the double stain, there is a lot> of background stain from the DAB. We do wash well so that is not the> problem. I am wondering if is the charge on the slides. I have checked> the pH of all the solutions and they are okay. Any ideas. The stain is> readable, but does not look too nice.> thanks> Diana> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > __________________________________________________> Do You Yahoo!?> Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ R U Ready for Windows Live Messenger Beta 8.5? Try it today! http://entertainment.sympatico.msn.ca/WindowsLiveMessenger From gu.lang <@t> gmx.at Fri Oct 26 11:02:57 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Oct 26 11:03:05 2007 Subject: AW: [Histonet] curious about soaking paraffin blocks. In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBF0222F037@wahtntex2.waht.swest.nhs.uk> Message-ID: <000301c817e9$ad120fc0$0202fea9@dielangs.at> To start at the beginning again. Do the US-histotechs cool the blocks on a coolplate and subsequently soak them in ice-water or ammoniacal water? Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 From LSebree <@t> uwhealth.org Fri Oct 26 11:05:59 2007 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Fri Oct 26 11:06:04 2007 Subject: [Histonet] curious about soaking paraffin blocks. In-Reply-To: <000301c817e9$ad120fc0$0202fea9@dielangs.at> Message-ID: Our histotechs cool faced blocks on ice that is frozen in plastic storage containers. There is usually some melted ice water on top of the ice block so in essence the blocks are cooled and soaked at the same time. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Friday, October 26, 2007 11:03 AM To: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] curious about soaking paraffin blocks. To start at the beginning again. Do the US-histotechs cool the blocks on a coolplate and subsequently soak them in ice-water or ammoniacal water? Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ttruscot <@t> vetmed.wsu.edu Fri Oct 26 11:14:25 2007 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Fri Oct 26 11:14:34 2007 Subject: [Histonet] soaking paraffin blocks. In-Reply-To: <7DBCCC1FBC77C94F99F920D0CA6400B61E4132@exchsrv01.barrynet.barry.edu> References: <86ADE4EB583CE64799A9924684A0FBBF0222F041@wahtntex2.waht.swest.nhs.uk> <7DBCCC1FBC77C94F99F920D0CA6400B61E4132@exchsrv01.barrynet.barry.edu> Message-ID: <35CF12B690D8CA4E95375A36B4E7B44CB6BD63@cvm36.vetmed.wsu.edu> Hi All, The art of histology would take more than a lifetime to perfect for every tissue. In my limited experience, I've come to believe that poor fixation is often blamed when poor processing is the real culprit. Poor processing will also cause tissues to "explode" on a waterbath. Optimal fixation and optimal processing can differ for each tissue and each species, so when you perform histology on a mix of tissues and species you try to find the average optimal protocol or two that you can fit into your schedule, then try to overcome problems with chilling or soaking or lower waterbath temps or whatever other tricks you've learned from experience or the histonet. Tom Truscott USDA Pullman, WA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith, Allen Sent: Friday, October 26, 2007 4:53 AM To: Kemlo Rogerson Cc: histonet@pathology.swmed.edu Subject: RE: [Histonet] soaking paraffin blocks. Yes, soaking the faced block is a cure for poor processing, but it works. I find that holding a moist ice cube against the block face for about 30 seconds does wonders for a poorly processed block. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo Rogerson Sent: Friday, October 26, 2007 7:24 AM To: Weems, Joyce; Kaliko, Bonnie; Mike Pence; Cheri Miller; Smith Wanda; Anna K. Schultz; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] curious about soaking paraffin blocks. "The key to the perfect section after soaking is not to leave the block so long that the tissue is damaged. The block then has to be faced to the exact spot that the perfect section can be taken - not where the tissue is still too soaked or after you've moved past that place again. Once again this part has to do with perception and experience." Helpful.... so obviously a way of answering poor fixation and/ or processing; sorry but I never came across this in the UK. Do any of you Brits do this? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Biology is the least of what makes someone a mother. --Oprah Winfrey This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Fri Oct 26 11:14:39 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Fri Oct 26 11:15:01 2007 Subject: [Histonet] alternative IHC marker other than GFAP for Message-ID: <002601c817eb$4fc03890$4101a8c0@carlba65530bda> Goeff, I would be interested to know which subtype of astrocytes are GFAP -ve. Thanks. Carl From JWEEMS <@t> sjha.org Fri Oct 26 11:18:03 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Oct 26 11:18:21 2007 Subject: [Histonet] curious about soaking paraffin blocks. In-Reply-To: <000301c817e9$ad120fc0$0202fea9@dielangs.at> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F4041@sjhaexc02.sjha.org> Yes... Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Gudrun Lang Sent: Friday, October 26, 2007 12:03 PM To: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] curious about soaking paraffin blocks. To start at the beginning again. Do the US-histotechs cool the blocks on a coolplate and subsequently soak them in ice-water or ammoniacal water? Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From rjbuesa <@t> yahoo.com Fri Oct 26 11:24:20 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 26 11:24:27 2007 Subject: [Histonet] soaking paraffin blocks. In-Reply-To: <35CF12B690D8CA4E95375A36B4E7B44CB6BD63@cvm36.vetmed.wsu.edu> Message-ID: <74469.69108.qm@web61215.mail.yahoo.com> Amen! Histology is art, and art is not science (even when medicine is an art helped by science). Ren? J. "Truscott, Tom" wrote: Hi All, The art of histology would take more than a lifetime to perfect for every tissue. In my limited experience, I've come to believe that poor fixation is often blamed when poor processing is the real culprit. Poor processing will also cause tissues to "explode" on a waterbath. Optimal fixation and optimal processing can differ for each tissue and each species, so when you perform histology on a mix of tissues and species you try to find the average optimal protocol or two that you can fit into your schedule, then try to overcome problems with chilling or soaking or lower waterbath temps or whatever other tricks you've learned from experience or the histonet. Tom Truscott USDA Pullman, WA __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From rjbuesa <@t> yahoo.com Fri Oct 26 11:27:52 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 26 11:27:59 2007 Subject: [Histonet] curious about soaking paraffin blocks. In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F4041@sjhaexc02.sjha.org> Message-ID: <45225.84570.qm@web61218.mail.yahoo.com> What I used to do is to place some water soaked paper towels ON the cool plate, and over the cooled soaked paper towel the faced off blocks. I also had one ice cube weapped in gauze to rub over the faced off block while in the chuck before taking the final ribbon. Ren? J. "Weems, Joyce" wrote: Yes... Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Gudrun Lang Sent: Friday, October 26, 2007 12:03 PM To: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] curious about soaking paraffin blocks. To start at the beginning again. Do the US-histotechs cool the blocks on a coolplate and subsequently soak them in ice-water or ammoniacal water? Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From gu.lang <@t> gmx.at Fri Oct 26 11:28:12 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Oct 26 11:28:18 2007 Subject: AW: [Histonet] curious about soaking paraffin blocks. In-Reply-To: Message-ID: <000701c817ed$341f65a0$0202fea9@dielangs.at> Linda, thank you for explaining. In Austria, we use coolplates. These tend to get a "snowcoat" after a few hours. The blocks sit in the more or less dry snow (and wait for their fate). To soften the tissue, if it is too brittle, we blow at the surface of the block (warm and humid). That works the most times. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: Sebree Linda A. [mailto:LSebree@uwhealth.org] Gesendet: Freitag, 26. Oktober 2007 18:06 An: gu.lang@gmx.at; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] curious about soaking paraffin blocks. Our histotechs cool faced blocks on ice that is frozen in plastic storage containers. There is usually some melted ice water on top of the ice block so in essence the blocks are cooled and soaked at the same time. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Friday, October 26, 2007 11:03 AM To: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] curious about soaking paraffin blocks. To start at the beginning again. Do the US-histotechs cool the blocks on a coolplate and subsequently soak them in ice-water or ammoniacal water? Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Fri Oct 26 11:30:27 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Oct 26 11:30:42 2007 Subject: [Histonet] curious about soaking paraffin blocks. In-Reply-To: <000701c817ed$341f65a0$0202fea9@dielangs.at> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F4044@sjhaexc02.sjha.org> And just as I thought... we are all full of hot air! Happy Friday and Happy NSH! j:>) Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Gudrun Lang Sent: Friday, October 26, 2007 12:28 PM To: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] curious about soaking paraffin blocks. Linda, thank you for explaining. In Austria, we use coolplates. These tend to get a "snowcoat" after a few hours. The blocks sit in the more or less dry snow (and wait for their fate). To soften the tissue, if it is too brittle, we blow at the surface of the block (warm and humid). That works the most times. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: Sebree Linda A. [mailto:LSebree@uwhealth.org] Gesendet: Freitag, 26. Oktober 2007 18:06 An: gu.lang@gmx.at; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] curious about soaking paraffin blocks. Our histotechs cool faced blocks on ice that is frozen in plastic storage containers. There is usually some melted ice water on top of the ice block so in essence the blocks are cooled and soaked at the same time. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Friday, October 26, 2007 11:03 AM To: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] curious about soaking paraffin blocks. To start at the beginning again. Do the US-histotechs cool the blocks on a coolplate and subsequently soak them in ice-water or ammoniacal water? Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From tkngflght <@t> yahoo.com Fri Oct 26 11:41:52 2007 From: tkngflght <@t> yahoo.com (Cheryl R. Kerry) Date: Fri Oct 26 11:42:02 2007 Subject: US Soaking blocks--dozens of ways RE: [Histonet] curious about soaking paraffin blocks. In-Reply-To: <000301c817e9$ad120fc0$0202fea9@dielangs.at> Message-ID: <002701c817ef$1d1f6b00$6801a8c0@CHERYLSLAPTOP> Gudrun, It varies by location and even by tech in a single location. Generally the blocks are cooled from their molten state on an embedding center cold plate and that's where the variances begin. Some facilities use wet ice either frozen filled in a plastic container or from and ice machine, some use the plastic histology trays and some use embedding station cold plates for each cutting station to keep blocks cold as they cut. Some facilities face the blocks before they cut, others don't. If they soak their blocks, some places use a second container of water and then put them on ice, others use just wet ice, still others use ammonia water (I've seen plastic containers filled with ammonia water and frozen to use as a block). Yet other facilities use saline to prevent swelling, and others use soapy water to cut surface tension to allow the moisture to penetrate more quickly. I've even seen a place that uses a dilute solution of Aerosol OT to soak. There a dozens of other ways I probably don't know--but most places use what works for them and allow each tech to adjust to get a good end product assuming that no harm is done and no tissue is lost due to the choices made. Hope this helps-- Cheryl Cheryl R. Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab, one great tech at a time. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Friday, October 26, 2007 11:03 AM To: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] curious about soaking paraffin blocks. To start at the beginning again. Do the US-histotechs cool the blocks on a coolplate and subsequently soak them in ice-water or ammoniacal water? Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Fri Oct 26 11:40:50 2007 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Fri Oct 26 11:42:33 2007 Subject: [Histonet] Re: Big Chief tablet? References: <009d01c8175b$e6aded00$0302a8c0@yourxhtr8hvc4p> Message-ID: <08A0A863637F1349BBFD83A96B27A50A120092@uwhis-xchng3.uwhis.hosp.wisc.edu> So make one! I don't think Wiki could be all it claims to be without it. :) Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Emily Sours Sent: Fri 10/26/2007 1:04 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Big Chief tablet? may i ask about "joe the toe"? i bet there isn't a wiki on that. Emily From ian.montgomery <@t> bio.gla.ac.uk Fri Oct 26 11:44:12 2007 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Fri Oct 26 11:44:20 2007 Subject: [Histonet] Calcium Message-ID: <007a01c817ef$70646770$6424d182@IBLS.GLA.AC.UK> This is a subject that was discussed several weeks ago, calcium staining. I'm staining culture cells that have been induced to produce calcium. So far I've been using alizarin red but with mixed success. I intend trying von Kossa but it's been a long time and then on lumps of bone. Has anyone tried the technique on culture cells and do they have any hints and tips? Ian. Dr. Ian Montgomery, Histotechnology, F.B.L.S., Support Unit, Thomson Building, University of Glasgow, G12 8QQ. From bwhitaker <@t> brownpathology.com Fri Oct 26 12:09:12 2007 From: bwhitaker <@t> brownpathology.com (Bonnie Whitaker) Date: Fri Oct 26 12:05:45 2007 Subject: [Histonet] curious about soaking paraffin blocks. In-Reply-To: Message-ID: <001401c817f2$eed26730$3601a8c0@brownpathology.net> Ditto.... chill and soak simultaneously on ice that has a little water on it. Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A. Sent: Friday, October 26, 2007 11:06 AM To: gu.lang@gmx.at; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] curious about soaking paraffin blocks. Our histotechs cool faced blocks on ice that is frozen in plastic storage containers. There is usually some melted ice water on top of the ice block so in essence the blocks are cooled and soaked at the same time. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Friday, October 26, 2007 11:03 AM To: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] curious about soaking paraffin blocks. To start at the beginning again. Do the US-histotechs cool the blocks on a coolplate and subsequently soak them in ice-water or ammoniacal water? Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jdmd77 <@t> hotmail.com Fri Oct 26 12:17:33 2007 From: jdmd77 <@t> hotmail.com (Julia Dahl) Date: Fri Oct 26 12:17:42 2007 Subject: [Histonet] Tryptase Stain for Mast Cell in Gastrointestinal Specimens In-Reply-To: <499236.27587.qm@web53605.mail.re2.yahoo.com> References: <745429.37353.qm@web90303.mail.mud.yahoo.com> <499236.27587.qm@web53605.mail.re2.yahoo.com> Message-ID: The use of mast cell tryptase has recently been touted to identify patients with diarrhea who may respond to anti-histamine treatment. This has been promulgated in advertisements to gastroenterologists - with the test being called a unique test offered by this ONE laboratory - the "ID-ME" test, as a test specific for mastocytic enterocolitis. The study was presented at the 2004 USCAP meeting and has not yet been widely accepted. Archives of Pathology and Laboratory Medicine: Vol. 130, No. 3, pp. 362¨C367.Mastocytic Enterocolitis: Increased Mucosal Mast Cells in Chronic Intractable Diarrhea Shriram Jakate, MD, FRCPath; Mark Demeo, MD; Rohan John, MD; Mary Tobin, MD; Ali Keshavarzian, MD From the Departments of Pathology (Drs Jakate and John) and Medicine (Drs Demeo, Tobin, and Keshavarzian), Rush University Medical Center, Chicago, IllAccepted October 28, 2005 Here is the Materials and Methods section: Forty-seven patients (32 women and 15 men; age range, 21-78 years) were diagnosed as having chronic persistent diarrhea of unknown cause at our institution between 2000 and 2004. All patients underwent the investigational protocol recommended by the American Gastroenterological Association. A comprehensive history was obtained that included the onset, pattern, and duration of symptoms, as well as the presence or absence of fecal incontinence, abdominal pain, weight loss, medication use, recent travel, diet, and stress. A physical examination was conducted to assess the extent of fluid loss, as well to evaluate rashes, flushing, oral ulcers, edema, perianal skin, anal sphincter tone, abdominal masses, and ascites. Complete blood counts and a basic metabolic panel (serum sodium, potassium, chloride, carbon dioxide, glucose, calcium, urea nitrogen, and creatinine) were performed. A stool analysis assessed the type and severity of diarrhea and the presence of ova and parasites. In addition, the patients underwent full colonoscopy (22/47 patients), upper endoscopy (20 patients), or both (5 patients), with random biopsy samples taken from the colon and from the second part of the duodenum. The random colonic biopsies included 4 to 10 pieces taken from the right and left colon and submitted in one container, and the random duodenal biopsies included 2 to 5 pieces taken mainly from the second part of the duodenum and submitted in one container. The colonic and duodenal biopsy specimens were processed for routine histologic examination with standard formalin fixation and paraffin embedding, and 5-¦Ìm-thin sections were stained with hematoxylin-eosin. In addition, all sections were immunohistochemically stained for MCT as follows: 4-¦Ìm-thin sections were cut, dried, and deparaffinized before placing them on the Ventana NexES autostainer (Ventana Medical Systems Inc, Tucson, Ariz), where they were treated with protease 1 for 4 minutes and then incubated with monoclonal mouse anti-human MCT (clone AA1, code M 7052; DAKO, Carpinteria, Calif; dilution 1: 1600) for 32 minutes. A recommended negative control was used. Visualization was performed using the iView DAB detection kit (Ventana Medical Systems Inc). The results: Thirty-three (70%) of 47 study patients with chronic intractable diarrhea had increased mast cells (mean ¡À SD, 25.7 ¡À 4.5 cells per high-power field) (Figure 5 ). Figure 6 illustrates the mean ¡À SD concentrations of mast cells in the control subjects, in the patients with diarrhea caused by specific diseases, and in the study patients with chronic intractable diarrhea. Among the 5 patients who underwent both colonic and duodenal biopsies, the increase in mast cells observed in 3 patients was seen in both organs, suggesting a diffuse enterocolonic mucosal increase. Among all biopsy samples in which there was an increase in mast cells, the increase was generally diffuse in the mucosa, with little elevation in the concentration of submucosal mast cells (Figure 7 ), with no sex differences observed in the control versus the affected populations. At follow-up, 22 (67%) of 33 study patients who received H1 and H2 receptor antagonists with or without mast cell mediator release inhibitor showed cessation of diarrhea or significant reduction in diarrhea (defined as 50% reduction in stool frequency or as 50% improvement in stool consistency). It states the type of antibody used for the study, above. While the study is interesting, it does not allow the distinction between whether the mast cells are the cause of the diarrhea or just a participant in whatever the process that is causing the diarrhea is. A specific problem with the study is that patient's biopsy sites were not specifically separated by right colon or left colon to determine statistical significance. Inflammatory cells of ALL TYPES are considerably more dense in the right colon and varies significantly with age. This study could simply have documented more mast cells in the patients whose right colon was biopsied more than the left colon. There are many reasons why patients could be responding to the drugs, as well - and they may or may not have ANYTHING to do with the presence or absence of mast cells. For instance for H1 reversible competitive antagonists of histamine at H1 receptor sites: They do not prevent histamine release or bind to the histamine that has already been released. The H1 receptor blockade results in decreased vascular permeability, reduction of pruritus and relaxation of smooth muscle in the respiratory and gastrointestinal tracts. That these patients respond to the drug could be an incident of TRUE, TRUE and UNRELATED. Because there is a "new" advertised test going out to your clinicians, gastroenterologists may be asking your pathologists about whether or not they do this test. They may think that your pathologists aren't staying up to date because they don't offer it. As a GI pathologist, I don't offer this test unless requested. I spend time with my clinicians telling them why I don't do the test. If they still want the test, I insist that they do the following: EGD with biopsies of small bowel, antrum and body (to evaluate for other causes of diarrhea) and colonoscopy with biopsied of TI, right colon and left colon SEPARATELY, and rectum. With those 7 biopsies, I can generally tell them why the patient has diarrhea - and if they comply with the Right versus LEFT colon, I can show them the mast cell difference between right and left colon and let them decide whether or not to try antihistamines - for whatever reason. Off my soap box now, Julia Dahl, M.D. Medical Director Mosaic GI-Hepatic Pathology Services Collierville, TN > Date: Fri, 26 Oct 2007 06:08:15 -0700 > From: slappycraw@yahoo.com > To: sccrshlly@yahoo.com; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Tryptase Stain for Mast Cell in Gastrointestinal Specimens > CC: > > We use Abcam ab2378 for human and monkey tissue with anti mouse polymer from dako and it works very well. > > Shelly Coker wrote: Hello everyone, > > Our pathologist has asked me to inquire about a Tryptase stain for mast cell tumors in gastro specimens. She said is one of those "latest/greatest" things. Does anyone have any experience with this stain? > > Thanks, > > Shelly > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > Larry A. Woody > Amgen > Seattle, Wa. > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Help yourself to FREE treats served up daily at the Messenger Caf¨¦. Stop by today. http://www.cafemessenger.com/info/info_sweetstuff2.html?ocid=TXT_TAGLM_OctWLtaglineFrom CIngles <@t> uwhealth.org Fri Oct 26 12:30:29 2007 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Fri Oct 26 12:30:34 2007 Subject: [Histonet] soaking paraffin blocks. References: <74469.69108.qm@web61215.mail.yahoo.com> Message-ID: <08A0A863637F1349BBFD83A96B27A50A120093@uwhis-xchng3.uwhis.hosp.wisc.edu> Let's not also forget the fact of life that speed is usually one of the most important things to consider. TAT has to usually be 1-2 days, no matter the size or type of tissue. Yes breadloafing, etc. helps fixation, but the fact remains that fixation/processing time is sometimes sacrificed for the speed of finished slide delivery. Unless the tissue is grossly unproccessed, it is rarely reproccessed because of the time factor. The little tricks we do in between are just a stopgap to try and give the best results on an undesirable situation. Not to mention the fact that the pathologist usually just don't get the importance of the fixation and processing steps. (except you, Kemlo!). Not meaning to throw everything on their shoulders again. I understand the reasoning behind their requirements. It just annoys me (and probably a few others too) that they are willing to possibly sacrifice a diagnosis just to get cases done in a "reasonable" amount of time. I'm sure if someone developed a way to present paraffin quality sections in a frozen section amount of time, they would be richer than Bill Gates -- and everyone would be much happier. Sorry, my philosophical is coming out again. And it's Friday. Everyone Who is going to NHS have fun at the company parties and get lots of free stuff. Don't forget too, that Denver also has a great Natural History Museum and of course the Denver Mint. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Fri 10/26/2007 11:24 AM To: Truscott, Tom; Smith, Allen; Kemlo Rogerson Cc: histonet@pathology.swmed.edu Subject: RE: [Histonet] soaking paraffin blocks. Amen! Histology is art, and art is not science (even when medicine is an art helped by science). Ren? J. From JWEEMS <@t> sjha.org Fri Oct 26 12:40:17 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Oct 26 12:40:38 2007 Subject: [Histonet] soaking paraffin blocks. In-Reply-To: <08A0A863637F1349BBFD83A96B27A50A120093@uwhis-xchng3.uwhis.hosp.wisc.edu> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F404A@sjhaexc02.sjha.org> In defense of the pathologists, it is hospital administration that has to get the case billed that pushes this rush. I am always in trouble for late charges! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Ingles Claire Sent: Friday, October 26, 2007 1:30 PM To: histonet@pathology.swmed.edu Subject: RE: [Histonet] soaking paraffin blocks. Let's not also forget the fact of life that speed is usually one of the most important things to consider. TAT has to usually be 1-2 days, no matter the size or type of tissue. Yes breadloafing, etc. helps fixation, but the fact remains that fixation/processing time is sometimes sacrificed for the speed of finished slide delivery. Unless the tissue is grossly unproccessed, it is rarely reproccessed because of the time factor. The little tricks we do in between are just a stopgap to try and give the best results on an undesirable situation. Not to mention the fact that the pathologist usually just don't get the importance of the fixation and processing steps. (except you, Kemlo!). Not meaning to throw everything on their shoulders again. I understand the reasoning behind their requirements. It just annoys me (and probably a few others too) that they are willing to possibly sacrifice a diagnosis just to get cases done in a "reasonable" amount of time. I'm sure if someone developed a way to present paraffin quality sections in a frozen section amount of time, they would be richer than Bill Gates -- and everyone would be much happier. Sorry, my philosophical is coming out again. And it's Friday. Everyone Who is going to NHS have fun at the company parties and get lots of free stuff. Don't forget too, that Denver also has a great Natural History Museum and of course the Denver Mint. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Fri 10/26/2007 11:24 AM To: Truscott, Tom; Smith, Allen; Kemlo Rogerson Cc: histonet@pathology.swmed.edu Subject: RE: [Histonet] soaking paraffin blocks. Amen! Histology is art, and art is not science (even when medicine is an art helped by science). Ren? J. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From victor <@t> pathology.washington.edu Fri Oct 26 14:52:32 2007 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Fri Oct 26 14:52:36 2007 Subject: [Histonet] Joe The Toe Message-ID: <47224580.7070608@pathology.washington.edu> Joe, Don't you have exclusive rights to the phrase? I'm sure you could find a lawyer to take the case. Have fun in Denver. http://www.kmkelly.com/crowley/20020907joe/JoetheToe.htm -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From michelle-griffin-reyes <@t> uiowa.edu Fri Oct 26 15:07:59 2007 From: michelle-griffin-reyes <@t> uiowa.edu (Griffin-Reyes, Michelle A) Date: Fri Oct 26 15:08:03 2007 Subject: [Histonet] Eosinophil Staining Message-ID: I am looking to do a comparative staining study on eosinophils (tissue is in FFPE). Among several stains, I plan to include a hematoxylin/eosin/azure II stain which will stain eosinophils pink (Friend et al. 2000) and a Sirius red stain (Aust et al. 2000). Unfortunately, for the H&E/azure II stain, no publications give the protocol or specific stain sequence. As for the Sirius red stain, the study states that the authors bought the stain from Bayer AG in Germany. I have contacted Bayer and no one seems to know what I am talking about. Before I substitute the "Bayer" Sirius red, I want to make sure that using another company's stain is appropriate as the protocol is described as "Sirius red (500mg) was dissolved in 45 ml aqua bidest, 50 ml absolute ethanol and 1 ml 1% NaOH; 4ml NaCl at 20% solution was added until slight precipitation occurred." My main concern is that the Sirius red I can order from other company's state there is little to no solubility in ethanol. I am hoping someone will be able to help me in regards to these questions. Thank you for your time. Michelle Griffin-Reyes Comparative Pathology Laboratory University of Iowa Hospitals and Clinics From jkiernan <@t> uwo.ca Fri Oct 26 15:43:36 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Oct 26 15:43:40 2007 Subject: [Histonet] Eosinophil Staining In-Reply-To: References: Message-ID: Eosinophils stain well with eosin! If used in an alkaline solution (pH 8 to 9) , eosinophils are the only stained objects in most tissues. (Paneth cell granules and tails of spermatozoa also stain but are unlikely to cause confusion.) Combining H&E with azure II (a mixture of blue cationic thiazine dyes) does not make much sense because the azure and the haemalum both colour nuclei blue, albeit for different reasons. You could use haemalum amd eosin or Lillie's azure-eosin (an excellent method, better than H&E, using azure A and eosin B). With these methods eosiniphil granules will be red; cytoplasm and collagen pink, nuclei blue-purple - not the best contrast if you want to see all the eosinophils easily at low magnification. There is more than one dye called sirius red, and their staining properties are not the same because the coloured anions vary greatly in size. In general, eosinophils stand out in sections stained by any anionic dye that can be used at pH 8-9. Biebrich scarlet is good, because it stays the same colour over a wide pH range. John Kiernan Anatomy, UWO London, Canada === ----- Original Message ----- From: "Griffin-Reyes, Michelle A" Date: Friday, October 26, 2007 16:09 Subject: [Histonet] Eosinophil Staining To: histonet@lists.utsouthwestern.edu > I am looking to do a comparative staining study on eosinophils (tissue > is in FFPE). Among several stains, I plan to include a > hematoxylin/eosin/azure II stain which will stain eosinophils pink > (Friend et al. 2000) and a Sirius red stain (Aust et al. 2000). > Unfortunately, for the H&E/azure II stain, no publications give the > protocol or specific stain sequence. As for the Sirius red > stain, the > study states that the authors bought the stain from Bayer AG in > Germany.I have contacted Bayer and no one seems to know what I > am talking about. > Before I substitute the "Bayer" Sirius red, I want to make sure that > using another company's stain is appropriate as the protocol is > described as "Sirius red (500mg) was dissolved in 45 ml aqua > bidest, 50 > ml absolute ethanol and 1 ml 1% NaOH; 4ml NaCl at 20% solution > was added > until slight precipitation occurred." My main concern is that > the Sirius > red I can order from other company's state there is little to no > solubility in ethanol. I am hoping someone will be able to help > me in > regards to these questions. Thank you for your time. > > Michelle Griffin-Reyes > Comparative Pathology Laboratory > University of Iowa Hospitals and Clinics > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From llewllew <@t> shaw.ca Fri Oct 26 16:15:34 2007 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Fri Oct 26 16:16:35 2007 Subject: [Histonet] Eosinophil Staining References: Message-ID: <002a01c81815$58f0e020$05024246@yourlk4rlmsu> The sirius red method you describe was originally published as a technique for amyloid. It was only incidentally noted that it demonstrates eosinophils well. You can leave out the sodium chloride if it is used solely for that purpose. It is found at: http://stainsfile.info/StainsFile/stain/amyloid/siriusllew.htm, and the dye is identified specifically at: http://stainsfile.info/StainsFile/dyes/35780.htm Bryan Llewellyn ----- Original Message ----- From: "Griffin-Reyes, Michelle A" To: Sent: Friday, October 26, 2007 1:07 PM Subject: [Histonet] Eosinophil Staining I am looking to do a comparative staining study on eosinophils (tissue is in FFPE). Among several stains, I plan to include a hematoxylin/eosin/azure II stain which will stain eosinophils pink (Friend et al. 2000) and a Sirius red stain (Aust et al. 2000). Unfortunately, for the H&E/azure II stain, no publications give the protocol or specific stain sequence. As for the Sirius red stain, the study states that the authors bought the stain from Bayer AG in Germany. I have contacted Bayer and no one seems to know what I am talking about. Before I substitute the "Bayer" Sirius red, I want to make sure that using another company's stain is appropriate as the protocol is described as "Sirius red (500mg) was dissolved in 45 ml aqua bidest, 50 ml absolute ethanol and 1 ml 1% NaOH; 4ml NaCl at 20% solution was added until slight precipitation occurred." My main concern is that the Sirius red I can order from other company's state there is little to no solubility in ethanol. I am hoping someone will be able to help me in regards to these questions. Thank you for your time. Michelle Griffin-Reyes Comparative Pathology Laboratory University of Iowa Hospitals and Clinics _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtarango <@t> nvcancer.org Fri Oct 26 17:48:09 2007 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Fri Oct 26 17:49:18 2007 Subject: [Histonet] curious about soaking paraffin blocks. In-Reply-To: Message-ID: <5AEC610C1CE02945BD63A395BA763EDE011B6F11@NVCIEXCH02.NVCI.org> If you process animal tissue on a human processing schedule, it definitely will come out very dry. I remember trying to cut fish brains from some fish that washed ashore a few years ago. As soon as the tissue hit the knife, it all turned to powder. I had to let the blocks float on the warm waterbath, sit in soapy ammonia water, and huff and puff all over to get a nice section. Also, a researcher here made some antibodies against a synthetic peptide of a nuclear transcription factor that he is studying. The sequence he used was the same for both the human and the mouse versions of the protein; however, I couldn't get the antibody to work on mouse tissue, until I modified the processing protocol such that the tissue came out looking like human tissue (which just took cutting the dehydration time). I just think animal tissue doesn't need to be processed as long as human tissue does. Maybe we humans drink more water or something. Mark Adam Tarango HT(ASCP) Histology & IHC Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 Direct Line (702) 822-5112 Treo (702) 759-9229 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kaliko, Bonnie Sent: Friday, October 26, 2007 6:28 AM To: Kemlo Rogerson; Weems, Joyce; Mike Pence; Cheri Miller; Smith Wanda; Anna K. Schultz; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] curious about soaking paraffin blocks. Kemlo, you obviously do not work with animal tissue which is VERY dry. It has nothing to do with poor fixation or over dehydration. Liver, bone, spleen, muscle is impossible to cut unless it is placed face down on ice. If you ever try cutting animal tissue and manage to get a good section without soaking it, then you should apply for a patent application. -----Original Message----- From: Kemlo Rogerson [mailto:Kemlo.Rogerson@waht.swest.nhs.uk] Sent: Friday, October 26, 2007 7:24 AM To: Weems, Joyce; Kaliko, Bonnie {Nonc~Nutley}; Mike Pence; Cheri Miller; Smith Wanda; Anna K. Schultz; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] curious about soaking paraffin blocks. "The key to the perfect section after soaking is not to leave the block so long that the tissue is damaged. The block then has to be faced to the exact spot that the perfect section can be taken - not where the tissue is still too soaked or after you've moved past that place again. Once again this part has to do with perception and experience." Helpful.... so obviously a way of answering poor fixation and/ or processing; sorry but I never came across this in the UK. Do any of you Brits do this? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Biology is the least of what makes someone a mother. --Oprah Winfrey This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From amosbrooks <@t> gmail.com Fri Oct 26 18:24:10 2007 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Fri Oct 26 18:24:15 2007 Subject: [Histonet] background staining after DAB Message-ID: <582736990710261624m19d830c7j42820417d0078b11@mail.gmail.com> Diana, Did you happen to use a tissue adhesive in your waterbath? The sticky adhesives often hold on to the IHC reagents and show up as a film around the sections. Good luck troubleshooting, Amos Brooks Message: 2 Date: Thu, 25 Oct 2007 14:02:50 -0400 From: "Diana McCaig" Subject: [Histonet] background staining after DAB To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi We are using the Biocare IHC protocol and using Fisher superfrost plus slides (due to decloaking). When doing the double stain, there is a lot of background stain from the DAB. We do wash well so that is not the problem. I am wondering if is the charge on the slides. I have checked the pH of all the solutions and they are okay. Any ideas. The stain is readable, but does not look too nice. thanks Diana From es144131 <@t> bcm.tmc.edu Sat Oct 27 01:23:36 2007 From: es144131 <@t> bcm.tmc.edu (Stephens, Elizabeth Humes) Date: Sat Oct 27 01:23:41 2007 Subject: [Histonet] computerized computation of IHC intensity Message-ID: <10C24F7C4D05EB45B5F0E1B397897849022B651C@BCMEVS7.ad.bcm.edu> Can I get people's advice on computerized grading of IHC intensity? That is, if I circle a region of the slide I'd like it to calculate staining intensity for that area. Is there a program that people have had success with? We have ImagePro but I'm not very familiar with it. I do my IHC staining without Ni (so it's brown) with a hemotoxylin counterstain--so I guess I would need to subtract "blue" from the image. Image brightness Do people have problems with the image brightness affecting results? Thank you so much! Elizabeth Stephens From debbie72367 <@t> yahoo.com Sat Oct 27 12:15:53 2007 From: debbie72367 <@t> yahoo.com (debbie craig) Date: Sat Oct 27 12:16:02 2007 Subject: [Histonet] Wanting to buy ASCP Histotechnology BOR Study Guide Message-ID: <531155.19805.qm@web57013.mail.re3.yahoo.com> Wondering if anyone has theASCP Histotechnology BOR study guide(the Purple book) that they would want to sell. __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From debbie72367 <@t> yahoo.com Sat Oct 27 12:34:13 2007 From: debbie72367 <@t> yahoo.com (debbie craig) Date: Sat Oct 27 12:34:17 2007 Subject: [Histonet] Want to Buy ASCP Histotechnology BOR Study Guide Message-ID: <323418.21737.qm@web57003.mail.re3.yahoo.com> Just wondering if anyone has th ASCP Histotechnology BOR Study guide that they would like to sell. __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From anthony <@t> histotechexchange.com Sat Oct 27 14:06:51 2007 From: anthony <@t> histotechexchange.com (anthony@histotechexchange.com) Date: Sat Oct 27 14:09:13 2007 Subject: [Histonet] Kansas Temporary or Temp to Perm Histolgoy Position Message-ID: <1731.65.196.168.131.1193512011.squirrel@host7.wfdns.com> Dear All: We currently have a temp or temp-to-perm position in Hays, KS that requires an ASCP-certified Histologist with grossing experience to work in a reference laboratory. The position is 11:30 a.m. to 8:00 p.m., Wednesday to Sunday. The position is split between embedding in the morning with grossing in the afternoon. If anyone out there is interested in learning to gross, the Histotech Exchange can provide full pay training to the right candidate. The position has a flexible start date of +- 2-3 weeks around the 21st of November. The Histotech Exchange is dedicated to patient care. Run by histologists for histologists, we need people who can get on with others and change their work practice to suit the needs of the client. Hays, KS, is a small town with a low cost of living, great restaurants, and plenty of shopping. We are offering accommodation and meals & incidental per diems as well as a great rate of pay. Please contact us for more information. Yours truly, Anthony Williams HT (ASCP) Histotech Exchange LLC 19 Whitmore Street Lexington, VA 24450 T 1 877 464 8911 F 1 540 301 0071 anthony@histotechexchange.com www.histotechexchange.com From ap_singh82 <@t> yahoo.co.in Sat Oct 27 23:27:56 2007 From: ap_singh82 <@t> yahoo.co.in (amritpal singh) Date: Sat Oct 27 23:28:02 2007 Subject: [Histonet] Help regarding staining of BBB Message-ID: <622416.64053.qm@web8511.mail.in.yahoo.com> Dear all, warm regards to you. I am here to find help from some body who has experience in staining of blood brain barrier as it is entirely a new area for my research group. We have heard of India ink used to stain BBB, can any body provide me the detailed procedure for it? Looking for your kind guidance. I think you a lot in anticipation for your cooperation. Regards, Amrit Pal Singh --------------------------------- Why delete messages? Unlimited storage is just a click away. From stamptrain <@t> yahoo.com Sun Oct 28 13:47:39 2007 From: stamptrain <@t> yahoo.com (Roger Moretz) Date: Sun Oct 28 13:47:44 2007 Subject: [Histonet] Help regarding staining of BBB In-Reply-To: <622416.64053.qm@web8511.mail.in.yahoo.com> Message-ID: <310323.42497.qm@web55815.mail.re3.yahoo.com> A quick Google search for "BBB staining" brings up over 400,000 hits. I suspect a similar search on PUBMED would bring up nearly as many (without some of the dupicates and irrelevant hits in Google). One of the first papers I saw was from 1988 where they used Evans Blue. Most of the India Ink and Evans Blue methods papers are from the 1950s and 1960s -- but I don't know if there are any books with compilations of the methods (there probably are--I just don't know where). EB is probably a better choice for light microscopy, since it does label the endothelium. Any leakage of course will be evidence of damage to the BBB. I no longer have access to all my reprints and reference manager, but this quick Google search shows that there are more than one way to get answers. Roger Moretz, Ph.D. Electron Microscopist (retired) "I'm not dead yet" Monty Python, 'Search for the Holy Grail' --- amritpal singh wrote: > Dear all, warm regards to you. I am here to find > help from some body who has experience in staining > of blood brain barrier as it is entirely a new area > for my research group. We have heard of India ink > used to stain BBB, can any body provide me the > detailed procedure for it? > > Looking for your kind guidance. I think you a lot > in anticipation for your cooperation. > > Regards, > Amrit Pal Singh > > > --------------------------------- > Why delete messages? Unlimited storage is just a > click away. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From jkiernan <@t> uwo.ca Sun Oct 28 17:37:55 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Sun Oct 28 17:38:00 2007 Subject: [Histonet] Help regarding staining of BBB In-Reply-To: <622416.64053.qm@web8511.mail.in.yahoo.com> References: <622416.64053.qm@web8511.mail.in.yahoo.com> Message-ID: The term blood-brain barrier refers to the failure of substances to pass from the blood into the nervous tissue of the CNS. Such substances include most large molecules such as plasma proteins, and dyes that bind to plasma proteins. The older dyes used for the purpose, such as Evans blue and trypan blue, can be lost in the preparation of sections. From about the 1960s, the popular tracers have been horseradish peroxidase (HRP; don't use the cheapest grades, which contain toxic impurities; Sigma Type 6 is OK) and labelled plasma proteins. Rhodamine-conjugated bovine albumin is excellent. For HRP you need to cut frozen sections. Rhodamine-albumin is fine with paraffin. Another approach is simply to immunostain sections for endogenous plasma proteins. John Kiernan Anatomy, UWO London, Canada. === ----- Original Message ----- From: amritpal singh Date: Sunday, October 28, 2007 0:30 Subject: [Histonet] Help regarding staining of BBB To: histonet@lists.utsouthwestern.edu > Dear all, warm regards to you. I am here to find help from some > body who has experience in staining of blood brain barrier as it > is entirely a new area for my research group. We have heard of > India ink used to stain BBB, can any body provide me the > detailed procedure for it? > > Looking for your kind guidance. I think you a lot in > anticipation for your cooperation. > > Regards, > Amrit Pal Singh > > > --------------------------------- > Why delete messages? Unlimited storage is just a click away. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From AnthonyH <@t> chw.edu.au Sun Oct 28 17:59:21 2007 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Oct 28 18:00:06 2007 Subject: [Histonet] curious about soaking paraffin blocks. Message-ID: Hi Kemlo, Yes, I'm one of those that routinely soak trimmed blocks on water/ice. The rationale being that some tissues (eg spleen with lots of blood and thyroid with copious amounts of colloid) over-dehydrate during processing resulting in cracks and shrinkage on the final microscopic appearance. These tissues benefit from water soaking (30 years experience confirms this). I agree that tissues should be properly fixed and processed but in the real-world where histo specimens are batched, processing times are optimised for the majority of specimens so many do require some tweaking at the microtomy stage. We used to regularly have small endoscopic biopsies that were over-processed. We now use a dedicated carousel-type processor for these specimens, and the dried-out appearance is now rarely seen. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo Rogerson Sent: Friday, 26 October 2007 4:57 PM To: Heckford, Karen - SMMC-SF; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] curious about soaking paraffin blocks. "I always face my blocks and then place them on the ice facing upwards. If I need to soak any blocks I use ammonia water only for about a minute. I have in the past used Downey fabric softener (the original scent only) this works great on big tissue like uterus. It also makes the lab smell like a laundry facility." Now that makes sense as you are alluding to 'softening' the tissue with fabric softener which you must do from time to time (but surely not regularly) for those very hard blocks. I've always maintained that if you fix tissue properly and process them optimally you won't need these fancy reclamation methods. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Biology is the least of what makes someone a mother. --Oprah Winfrey This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From spotts <@t> aperio.com Mon Oct 29 00:13:01 2007 From: spotts <@t> aperio.com (Steven Potts) Date: Mon Oct 29 00:13:17 2007 Subject: [Histonet] Application Specialist employment openings for persons with histology lab background Message-ID: <52F0A1B1F834C54DB1C0CF32182D6E9B01931AEF@sagan.aperio.int> Dear All, We are looking for several people with histology or pathology lab background, who love working with computers, to join our team at Aperio Technologies. Aperio is the leader in digital pathology, including full-slide scanning, a website for remote management/annotation of slides, and IHC algorithms. We are looking for several Application Specialists who can work with our pathology and histology customers to customize typical lab workflows, help explain immunohistochemistry algorithms, and translate histopathology needs back to our engineering team. Positions can work remotely, provided the Specialist is willing to travel to customer sites. We have openings in both the United States and Europe. More information at www.aperio.com . Best regards, Steve Steve Potts, Ph.D. Director, Biopharma Aperio Technologies Digital Pathology Solutions Conference North American Meeting October 21-23 Europe Meeting December 3-5 phone: (760) 539-1118 email: spotts@aperio.com web: www.aperio.com From Kemlo.Rogerson <@t> waht.swest.nhs.uk Mon Oct 29 02:45:26 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Oct 29 02:45:34 2007 Subject: [Histonet] soaking paraffin blocks. Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222F049@wahtntex2.waht.swest.nhs.uk> Amen! Histology is art, and art is not science (even when medicine is an art helped by science). Ren? J. Then Histologists are artists not scientists? Isn't art, science that hasn't yet been understood? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Biology is the least of what makes someone a mother. --Oprah Winfrey This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Kemlo.Rogerson <@t> waht.swest.nhs.uk Mon Oct 29 02:47:15 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Oct 29 02:47:22 2007 Subject: [Histonet] curious about soaking paraffin blocks. Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222F04A@wahtntex2.waht.swest.nhs.uk> "Since we process every organ of rat, mouse, dog, rabbit and monkey on many animals, processing by hand would be archaic. The amount of time each species is processed depends on the tissue size. Mouse having the shortest processing schedule. Monkey is the easiest to section because it is so close to human tissue. All tissue is fully fixed in 10% NBF before processing." Bonnie That's an interesting comment "Monkey is the easiest to section because it is so close to human tissue", is that because primate tissue is easier to process or that processing schedules are primarily focused on human material? Is there something in primate proteins that makes it easier to process than say rodent tissue? It said on my wireless this morning that someone wearing red socks had won something in North America; what was that? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Biology is the least of what makes someone a mother. --Oprah Winfrey This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From oshel1pe <@t> cmich.edu Mon Oct 29 07:42:54 2007 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Mon Oct 29 07:43:05 2007 Subject: [Histonet] curious about soaking paraffin blocks. In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBF0222F04A@wahtntex2.waht.swest.nhs.uk> References: <86ADE4EB583CE64799A9924684A0FBBF0222F04A@wahtntex2.waht.swest.nhs.uk> Message-ID: I've been reading the posts in this thread, and I doubt that the proteins have anything to do with the differences in processing schedules. I suspect it's more the fat content. Both the amount and the nature of saturated vs unsaturated fats. I suspect human tissue is generally fattier than other animals, so a dehydration schedule that works for people extracts too much fat from other animal tissues, making them "dry" and crumbly. If this is true, the same sort of difference should show up when sectioning tissues from, say, wild squirrels in the early spring (after they've used up their winter-survival fat stores) and squirrels in the late fall/early winter, when their fat stores should be maximal. Maybe. Any of the histotechs in Vet Med colleges noticed having to change processing schedules of wild or outdoor animals with the seasons? As to winning ... yeah, I heard something about that. Nothing important. Something about hitting sticks with balls, or the failure to hit them. Phil >"Since we process every organ of rat, mouse, dog, rabbit and monkey on >many animals, processing by hand would be archaic. The amount of time >each species is processed depends on the tissue size. Mouse having the >shortest processing schedule. Monkey is the easiest to section because >it is so close to human tissue. All tissue is fully fixed in 10% NBF >before processing." >Bonnie > >That's an interesting comment "Monkey is the easiest to section because >it is so close to human tissue", is that because primate tissue is >easier to process or that processing schedules are primarily focused on >human material? Is there something in primate proteins that makes it >easier to process than say rodent tissue? > >It said on my wireless this morning that someone wearing red socks had >won something in North America; what was that? > > >Kemlo Rogerson >Pathology Manager >DD 01934 647057 or extension 3311 >Mob 07749 754194; Pager 07659 597107; > >Biology is the least of what makes someone a mother. --Oprah Winfrey -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576 From rjbuesa <@t> yahoo.com Mon Oct 29 07:52:02 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Oct 29 07:52:08 2007 Subject: [Histonet] soaking paraffin blocks. In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBF0222F049@wahtntex2.waht.swest.nhs.uk> Message-ID: <341061.88874.qm@web61219.mail.yahoo.com> Exactly, we are artists using some help from the science that we do not really develop even if we may get to some results by try and error based on scientific knowledge and understanding. Michelangelo would have never cared much about the mathematical equations that could have described the curves in his sculptures, or the energy invested in all the chiselling he had to do to finish "Piet?". Ren? J. Kemlo Rogerson wrote: Amen! Histology is art, and art is not science (even when medicine is an art helped by science). Ren? J. Then Histologists are artists not scientists? Isn't art, science that hasn't yet been understood? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Biology is the least of what makes someone a mother. --Oprah Winfrey This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From Kemlo.Rogerson <@t> waht.swest.nhs.uk Mon Oct 29 08:11:49 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Oct 29 08:11:58 2007 Subject: [Histonet] curious about soaking paraffin blocks. Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222F05B@wahtntex2.waht.swest.nhs.uk> "I suspect human tissue is generally fattier than other animals, so a dehydration schedule that works for people extracts too much fat from other animal tissues, making them "dry" and crumbly." Philip Yes I believe that is the answer I've heard before; that it is not how the proteins react to the fixative but what stops the fixative from fixing the proteins in the first place. Fat delays the process of fixation; absence of fat doesn't. Probably accounts for processing too. Fat tissue takes longer to process than thin tissue. Isn't it hitting balls with sticks not 'hitting sticks with balls'? You'd have to keep the stick motionless and throw the ball at it; isn't it called cricket anyway? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Never does nature say one thing and wisdom another. --Juvenal This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From n.cragg <@t> epistem.co.uk Mon Oct 29 08:11:55 2007 From: n.cragg <@t> epistem.co.uk (Nicola Cragg) Date: Mon Oct 29 08:23:22 2007 Subject: [Histonet] Soaking paraffin blocks Message-ID: I have read with interest the discussion on soaking paraffin blocks and with fear of being struck down by hell, fire and damnation - Kemlo I have to admit I'm in the UK and I do it!! At least with some tissues - in particular intestinal bundles. After chilling blocks on the coldplate I sit them face down on ice with a little water (have a plastic tray in the freezer with ice in it). I routinely cut blocks containing 6 bundles of intestine, each bundle contains approx. 16 pieces of intestine all orientated end on to get numerous cross-sections through the intestine - they cut a lot easier after being sat on the wet ice and I have no complaints on the quality of my sectioning. What am I concerned with is the claim of poor processing - how does one know that it is poor processing, particularly if you haven't seen the blocks or attempted to cut them? What is poor about it - insufficient wax penetration, lack of dehydration? Processing is essentially quite a straight forward process and apart from varying timings in alcohols, xylene and wax, I can't understand how it could go so wrong?!! I have a Leica TP1020 without vacuum and run overnight schedule based on what I've read up - reagents are changed regularly always erring on the side of caution. I am a self-taught histologist (and there may lay your accusations - LOL) and have been "practicing" for over 5 years now, but I have only learnt by reading up on what the experts say and do (including Histonet - which I have found to be a fantastic tutor) and by practice. I am always keen to learn from my more experienced peers so please expand on pharses such as "poor processing" and "poor fixation". This message has been scanned for viruses by BlackSpider MailControl - www.blackspider.com From oshel1pe <@t> cmich.edu Mon Oct 29 08:23:42 2007 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Mon Oct 29 08:23:54 2007 Subject: [Histonet] curious about soaking paraffin blocks. In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBF0222F05B@wahtntex2.waht.swest.nhs.uk> References: <86ADE4EB583CE64799A9924684A0FBBF0222F05B@wahtntex2.waht.swest.nhs.uk> Message-ID: Oh? Ok hitting balls with sticks then. I seem to recall doing something like that in my misspent youth. Isn't cricket hitting little ball-shaped bombs with bats that look more like paddles? Usually played by robots? Phil >"I suspect human tissue is generally fattier than other animals, so a >dehydration schedule that works for people extracts too much fat from >other animal tissues, making them "dry" and crumbly." >Philip > >Yes I believe that is the answer I've heard before; that it is not how >the proteins react to the fixative but what stops the fixative from >fixing the proteins in the first place. Fat delays the process of >fixation; absence of fat doesn't. Probably accounts for processing too. >Fat tissue takes longer to process than thin tissue. > >Isn't it hitting balls with sticks not 'hitting sticks with balls'? >You'd have to keep the stick motionless and throw the ball at it; isn't >it called cricket anyway? > >Kemlo Rogerson >Pathology Manager >DD 01934 647057 or extension 3311 >Mob 07749 754194; Pager 07659 597107; >Never does nature say one thing and wisdom another. --Juvenal > >This e-mail is confidential and privileged. If you are not the intended >recipient please accept my apologies; please do not disclose, copy or >distribute information in this e-mail or take any action in reliance on >its contents: to do so is strictly prohibited and may be unlawful. >Please inform me that this message has gone astray before deleting it. >Thank you for your co-operation > From Kemlo.Rogerson <@t> waht.swest.nhs.uk Mon Oct 29 08:39:58 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Oct 29 08:40:06 2007 Subject: [Histonet] Soaking paraffin blocks Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222F05F@wahtntex2.waht.swest.nhs.uk> "What am I concerned with is the claim of poor processing - how does one know that it is poor processing, particularly if you haven't seen the blocks or attempted to cut them? What is poor about it - insufficient wax penetration, lack of dehydration? Processing is essentially quite a straight forward process and apart from varying timings in alcohols, xylene and wax, I can't understand how it could go so wrong?!! I have a Leica TP1020 without vacuum and run overnight schedule based on what I've read up - reagents are changed regularly always erring on the side of caution. I am a self-taught histologist (and there may lay your accusations - LOL) and have been "practicing" for over 5 years now, but I have only learnt by reading up on what the experts say and do (including Histonet - which I have found to be a fantastic tutor) and by practice. I am always keen to learn from my more experienced peers so please expand on pharses such as "poor processing" and "poor fixation"." Nicola Poor fixation could mean not enough or too much fixation. Some fixatives harden tissue when 'overexposed' some fixative take some time to penetrate and to fix. Bluntly, IMHO, you can't process properly unless you've fixed 'properly'. Fixation puts the tissue in the correct state to resist the effects of processing and to allow the fluids to penetrate. If the processing fluids are left on too long, tissue goes hard, too little and water or alcohol isn't removed. If the tissue isn't clear in xylene then it's not dehydrated, if it's not dehydrated then you won't get the wax in properly, if you don't get the xylene out then you make the wax and the tissue 'soggy'. Overprocess and you harden the tissue (usually the heat from the wax) and you have to use reclamation techniques such as ice/ water IMHO. You build a house from the foundations up; fixation is your foundation without which nothing underpins the processing. What does your Company do in the UK? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Never does nature say one thing and wisdom another. --Juvenal This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From alaskagirl1950 <@t> yahoo.com Mon Oct 29 08:50:47 2007 From: alaskagirl1950 <@t> yahoo.com (Patricia Adams) Date: Mon Oct 29 08:50:51 2007 Subject: [Histonet] soaking paraffin blocks. ART Message-ID: <540661.7094.qm@web52501.mail.re2.yahoo.com> Does this mean I will be worth more dead? Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D ----- Original Message ---- From: Rene J Buesa To: Kemlo Rogerson ; "Truscott, Tom" ; "Smith, Allen" Cc: histonet@pathology.swmed.edu Sent: Monday, October 29, 2007 7:52:02 AM Subject: RE: [Histonet] soaking paraffin blocks. Exactly, we are artists using some help from the science that we do not really develop even if we may get to some results by try and error based on scientific knowledge and understanding. Michelangelo would have never cared much about the mathematical equations that could have described the curves in his sculptures, or the energy invested in all the chiselling he had to do to finish "Piet?". Ren? J. Kemlo Rogerson wrote: Amen! Histology is art, and art is not science (even when medicine is an art helped by science). Ren? J. Then Histologists are artists not scientists? Isn't art, science that hasn't yet been understood? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Biology is the least of what makes someone a mother. --Oprah Winfrey This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From rjbuesa <@t> yahoo.com Mon Oct 29 09:04:08 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Oct 29 09:04:12 2007 Subject: [Histonet] soaking paraffin blocks. ART In-Reply-To: <540661.7094.qm@web52501.mail.re2.yahoo.com> Message-ID: <563757.49253.qm@web61218.mail.yahoo.com> Perhaps BUT remember: you are not dead until you are forgotten! Ren? J. Patricia Adams wrote: Does this mean I will be worth more dead? Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D ----- Original Message ---- From: Rene J Buesa To: Kemlo Rogerson ; "Truscott, Tom" ; "Smith, Allen" Cc: histonet@pathology.swmed.edu Sent: Monday, October 29, 2007 7:52:02 AM Subject: RE: [Histonet] soaking paraffin blocks. Exactly, we are artists using some help from the science that we do not really develop even if we may get to some results by try and error based on scientific knowledge and understanding. Michelangelo would have never cared much about the mathematical equations that could have described the curves in his sculptures, or the energy invested in all the chiselling he had to do to finish "Piet?". Ren? J. Kemlo Rogerson wrote: Amen! Histology is art, and art is not science (even when medicine is an art helped by science). Ren? J. Then Histologists are artists not scientists? Isn't art, science that hasn't yet been understood? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Biology is the least of what makes someone a mother. --Oprah Winfrey This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From gmartin <@t> marshallmedical.org Mon Oct 29 10:11:34 2007 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Mon Oct 29 10:11:42 2007 Subject: [Histonet] specimens from surgery Message-ID: <6ED9D4252F278841A0593D3D788AF24C011539CE@mailsvr.MARSHMED.local> The surgery department we work with is stating that the bags (manufacturer Bitran) are leaking in the surgery suites and that the staff cannot deal with the leaks and the smell. They would like to deliver all specimens fresh. My question is; how are those of you that receive specimens in a hospital from surgery receiving them. Thank you Gary California From victor <@t> pathology.washington.edu Mon Oct 29 10:15:34 2007 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Mon Oct 29 10:15:44 2007 Subject: [Histonet] specimens from surgery In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C011539CE@mailsvr.MARSHMED.local> References: <6ED9D4252F278841A0593D3D788AF24C011539CE@mailsvr.MARSHMED.local> Message-ID: <4725F916.5010704@pathology.washington.edu> Gary, All our specimens from surgery are received fresh, bagged and have been for years. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Martin, Gary wrote: > The surgery department we work with is stating that the bags > (manufacturer Bitran) are leaking in the surgery suites and that the > staff cannot deal with the leaks and the smell. They would like to > deliver all specimens fresh. > > My question is; how are those of you that receive specimens in a > hospital from surgery receiving them. > > > > Thank you > > Gary > > California > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From JWEEMS <@t> sjha.org Mon Oct 29 10:22:04 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Mon Oct 29 10:22:20 2007 Subject: [Histonet] specimens from surgery In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C011539CE@mailsvr.MARSHMED.local> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F405B@sjhaexc02.sjha.org> We supply the ORs with a variety of formalin filled containers from 120 ml to 170 ml. If the specimen is too large to fit the largest size, it is brought over fresh. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Martin, Gary Sent: Monday, October 29, 2007 11:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] specimens from surgery The surgery department we work with is stating that the bags (manufacturer Bitran) are leaking in the surgery suites and that the staff cannot deal with the leaks and the smell. They would like to deliver all specimens fresh. My question is; how are those of you that receive specimens in a hospital from surgery receiving them. Thank you Gary California _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From rjbuesa <@t> yahoo.com Mon Oct 29 10:28:14 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Oct 29 10:28:18 2007 Subject: [Histonet] specimens from surgery In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C011539CE@mailsvr.MARSHMED.local> Message-ID: <399116.99661.qm@web61215.mail.yahoo.com> We only provided the surgery suites with plastic containers with NBF for small specimens. Large speciemens were delivered fresh, they called us and we pick them up. No problems at all. Ren? J. "Martin, Gary" wrote: The surgery department we work with is stating that the bags (manufacturer Bitran) are leaking in the surgery suites and that the staff cannot deal with the leaks and the smell. They would like to deliver all specimens fresh. My question is; how are those of you that receive specimens in a hospital from surgery receiving them. Thank you Gary California _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From mpence <@t> grhs.net Mon Oct 29 10:39:22 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Mon Oct 29 10:39:29 2007 Subject: [Histonet] specimens from surgery In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C011539CE@mailsvr.MARSHMED.local> Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C784@IS-E2K3.grhs.net> We have a small room in surgery that they have 2- 5 gallon containers with 10% NBF in. They put their specimen in a appropriate sized container in the room. When the case is over the specimen is taken to the room and formalin is added and the specimen in placed in a container that we pick up 4 times a day. The containers range from urine cups to gallon size buckets with snap on lids. No leaks and no formalin in the OR rooms. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Gary Sent: Monday, October 29, 2007 10:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] specimens from surgery The surgery department we work with is stating that the bags (manufacturer Bitran) are leaking in the surgery suites and that the staff cannot deal with the leaks and the smell. They would like to deliver all specimens fresh. My question is; how are those of you that receive specimens in a hospital from surgery receiving them. Thank you Gary California _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From buck45 <@t> optonline.net Mon Oct 29 10:39:17 2007 From: buck45 <@t> optonline.net (buck45@optonline.net) Date: Mon Oct 29 10:39:32 2007 Subject: [Histonet] bleeding eosin Message-ID: Hi - I was wondering if anyone who uses xylene substitutes have had problems with the eosin bleeding out of the tissue. I don't know if it is the type and brand of eosin or something else. I have never used a substitute before and I find that the stainer needs to be changed much more that with regular xylene. I seem to get a lot of water on the slides unless I change it every other day. Gail Paramus, NJ From TMcNemar <@t> lmhealth.org Mon Oct 29 10:41:08 2007 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Mon Oct 29 10:40:49 2007 Subject: [Histonet] specimens from surgery In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F405B@sjhaexc02.sjha.org> Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F4E8@lmhsmail.lmhealth.org> This is exactly the way we do it only we supply much larger containers all the way up to about 3L. We rarely get anything fresh (except extremeties, entire bowels, etc. basically any thing that doesen't fit into the largest size container). We are a dayshift only operation with no evenings or weekends so there's no one here to to put formalin on them after hours. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Weems, Joyce Sent: Monday, October 29, 2007 11:22 AM To: Martin, Gary; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] specimens from surgery We supply the ORs with a variety of formalin filled containers from 120 ml to 170 ml. If the specimen is too large to fit the largest size, it is brought over fresh. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Martin, Gary Sent: Monday, October 29, 2007 11:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] specimens from surgery The surgery department we work with is stating that the bags (manufacturer Bitran) are leaking in the surgery suites and that the staff cannot deal with the leaks and the smell. They would like to deliver all specimens fresh. My question is; how are those of you that receive specimens in a hospital from surgery receiving them. Thank you Gary California _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From Shawna.Thomas <@t> Integris-Health.com Mon Oct 29 10:42:56 2007 From: Shawna.Thomas <@t> Integris-Health.com (Thomas, Shawna G.) Date: Mon Oct 29 10:43:02 2007 Subject: [Histonet] specimens from surgery In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C011539CE@mailsvr.MARSHMED.local> References: <6ED9D4252F278841A0593D3D788AF24C011539CE@mailsvr.MARSHMED.local> Message-ID: <0AF4F8EA51200F49ADC32E6E353F024306559858@EXCHANGE2-OKC.corp.integris-health.com> Our surgery department orders their own plastic containers for the tissues. We provide a 5 gallon cube of NBF for them to fill the containers after they obtain the tissue. They have a dedicated room for filling and labeling the containers. They leave the labeled specimen in the room and a lab assistant retrieves the specimens through out the day and brings them to histology for accessioning and grossing. If a specimen is for frozen or fresh (lymphoma/cultures) someone from the OR delivers it to histology. Shawna Baker, MBA, PA(ASCP) Pathologists' Assistant AmeriPath Oklahoma @ ISMC Pathology (405)644-6146 phone (405)636-7518 fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Gary Sent: Monday, October 29, 2007 10:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] specimens from surgery The surgery department we work with is stating that the bags (manufacturer Bitran) are leaking in the surgery suites and that the staff cannot deal with the leaks and the smell. They would like to deliver all specimens fresh. My question is; how are those of you that receive specimens in a hospital from surgery receiving them. Thank you Gary California _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This e-mail may contain identifiable health information that is subject to protection under state and federal law. This information is intended to be for the use of the individual named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this information is prohibited and may be punishable by law. If you have received this electronic transmission in error, please notify us immediately by electronic mail (reply). From jb481 <@t> columbia.edu Mon Oct 29 11:09:27 2007 From: jb481 <@t> columbia.edu (Joshua Berman) Date: Mon Oct 29 11:09:29 2007 Subject: [Histonet] Help! Gelatin embedded mouse brains cracking! Message-ID: <000b01c81a46$148c6fc0$3926a8c0@JoshB> I've been using 10% gelatin/1xPBS to embed mouse brains prior to quick freezing in a cold isopentane bath. I like the gelatin embedding because I can see the orientation of the brain in the block and trim it to optimize orientation. The only problem I've had is that sometimes (with varying frequency) the block (and sometimes the brain with it) cracks in the freezing bath. I have isopentane in a metal container surrounded by a dry ice methanol bath. I put one piece of dry ice in the isopentane. The 4% para perfused mouse brains are infiltrated with 25%, then 30% sucrose after postfixing in 4% para. The gelatin does not contain any sucrose and was not placed in para. The brain is placed in 37*C geatin in a cryomold, oriented, and then goes into the refrigerator for the gelatin to harden prior to trimming the block and freezing it. Is this temperature control? Differential rates of expansion? Do I need to add something to the gelatin or use more or less of it? Any suggestions? Thanks in advance. Joshua Berman Columbia University From mauger <@t> email.chop.edu Mon Oct 29 11:19:18 2007 From: mauger <@t> email.chop.edu (Joanne Mauger) Date: Mon Oct 29 11:19:58 2007 Subject: [Histonet] bleeding eosin Message-ID: Hi Gail, We use xylene, but recently used a toluene based mounting medium instead of our regular xylene based one. We have been having terrible eosin bleeding, and think it has to be something about the mountant, as nothing else is different. Maybe we have a common problem we can help each other troubleshoot. Tha mountant is from Fisher- Protocol secure mount lot#79931. Even the other Protocol- xylene based, lot#75461, is very watery, and seems not to have any resin it. The coverslips pop right off! Fisher claims not to be aware of any trouble, but since we are in the same area-Philadelphia& NJ- maybe we both have bad batches? Do you see any common product? Hope I didn't confuse the issue! Thanks I hope we can both resolve the issue. Jo Mauger From rjbuesa <@t> yahoo.com Mon Oct 29 11:24:30 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Oct 29 11:24:34 2007 Subject: [Histonet] bleeding eosin In-Reply-To: Message-ID: <531035.42442.qm@web61217.mail.yahoo.com> That is a known disadvantage of some xylene substitutes. Ren? J. buck45@optonline.net wrote: Hi - I was wondering if anyone who uses xylene substitutes have had problems with the eosin bleeding out of the tissue. I don't know if it is the type and brand of eosin or something else. I have never used a substitute before and I find that the stainer needs to be changed much more that with regular xylene. I seem to get a lot of water on the slides unless I change it every other day. Gail Paramus, NJ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From llewllew <@t> shaw.ca Mon Oct 29 11:31:12 2007 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Mon Oct 29 11:32:26 2007 Subject: [Histonet] curious about soaking paraffin blocks. References: <86ADE4EB583CE64799A9924684A0FBBF0222F05B@wahtntex2.waht.swest.nhs.uk> Message-ID: <003a01c81a49$1e5c7650$05024246@yourlk4rlmsu> It's actually rounders, not cricket. Bryan ----- Original Message ----- From: "Kemlo Rogerson" To: "Philip Oshel" ; Sent: Monday, October 29, 2007 6:11 AM Subject: RE: [Histonet] curious about soaking paraffin blocks. Isn't it hitting balls with sticks not 'hitting sticks with balls'? You'd have to keep the stick motionless and throw the ball at it; isn't it called cricket anyway? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Never does nature say one thing and wisdom another. --Juvenal This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Mon Oct 29 11:50:30 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Oct 29 11:50:39 2007 Subject: [Histonet] curious about soaking paraffin blocks. Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222F066@wahtntex2.waht.swest.nhs.uk> It's actually rounders, not cricket. Bryan I understand, Red Sox are a rounders team, isn't that a girl's game? Bit like Hockey, that's a girl's game too. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; If someone listens, or stretches out a hand, or whispers a kind word of encouragement, or attempts to understand a lonely person, extraordinary things begin to happen. --Loretta Girzaitis This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From KMENDELL <@t> nhs-healthlink.org Mon Oct 29 12:04:53 2007 From: KMENDELL <@t> nhs-healthlink.org (Kate Mendell) Date: Mon Oct 29 12:05:09 2007 Subject: [Histonet] Tissue falling off Message-ID: <4725DA75020000A80001AF2F@mail.nhs-healthlink.org> Thank-you to everyone that wrote and gave advise. What ever problem we were having has stopped. I think we are going to chalk it up to bad batch of slides. By the way erie scientific has been great! I have had numerous phone calls from the product manager willing to do what they can to help us figure this out. Included in that was a batch of slides with a new lot number. Seeing that we don't know if was a bad batch or one that sat too long on Rep shelf without roating stock. There was no Passing the Buck just willing to help solve problem. Now that is GREAT CUSTOMER SERVICE! This message and its contents are confidential and are intended for the use of the addressee only, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the intended recipient, this serves as notice that any unauthorized distribution, duplication, printing, or any other use is strictly prohibited. If you feel you have received this email in error, please delete the message and notify the sender so that we may prevent future occurrences. From llewllew <@t> shaw.ca Mon Oct 29 12:03:50 2007 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Mon Oct 29 12:05:20 2007 Subject: [Histonet] curious about soaking paraffin blocks. References: <86ADE4EB583CE64799A9924684A0FBBF0222F066@wahtntex2.waht.swest.nhs.uk> Message-ID: <004701c81a4d$ada254c0$05024246@yourlk4rlmsu> Well, a little kids' game, anyway. AND, we all caught the rounders ball with our bare hands, not in a padded, webbed, oversized mitt. Hockey in Canada is different from field hockey in the UK. Here it is a blood sport. We expect teeth to be knocked out at every game. Bryan ----- Original Message ----- From: "Kemlo Rogerson" To: "Bryan Llewellyn" ; "Histonet" Sent: Monday, October 29, 2007 9:50 AM Subject: RE: [Histonet] curious about soaking paraffin blocks. It's actually rounders, not cricket. Bryan I understand, Red Sox are a rounders team, isn't that a girl's game? Bit like Hockey, that's a girl's game too. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; If someone listens, or stretches out a hand, or whispers a kind word of encouragement, or attempts to understand a lonely person, extraordinary things begin to happen. --Loretta Girzaitis This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From JMacDonald <@t> mtsac.edu Mon Oct 29 13:38:07 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon Oct 29 13:41:21 2007 Subject: [Histonet] bleeding eosin In-Reply-To: <531035.42442.qm@web61217.mail.yahoo.com> Message-ID: Not all mounting medium is compatible with xylene substitutes. Certain combinations of mounting media and substitute can cause the bleeding of eosin. Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu Rene J Buesa Sent by: histonet-bounces@lists.utsouthwestern.edu 10/29/2007 09:24 AM To buck45@optonline.net, histonet@lists.utsouthwestern.edu cc Subject Re: [Histonet] bleeding eosin That is a known disadvantage of some xylene substitutes. Ren? J. buck45@optonline.net wrote: Hi - I was wondering if anyone who uses xylene substitutes have had problems with the eosin bleeding out of the tissue. I don't know if it is the type and brand of eosin or something else. I have never used a substitute before and I find that the stainer needs to be changed much more that with regular xylene. I seem to get a lot of water on the slides unless I change it every other day. Gail Paramus, NJ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From plott <@t> uab.edu Mon Oct 29 14:31:11 2007 From: plott <@t> uab.edu (Patricia F Lott) Date: Mon Oct 29 14:31:21 2007 Subject: [Histonet] mouse bones cleaned Message-ID: <4850136AECAE5447B2E7FF80CA9225300C34AA@UABEXMB4.ad.uab.edu> I have some mouse bones which have been cleaned by dermestid beetles, then placed into bleach for whitening, and stored in alcohol. Is there a way to make them last longer? I'm afraid if I just dry them now, they will be very fragile! From LINDA.MARGRAF <@t> childrens.com Mon Oct 29 15:44:53 2007 From: LINDA.MARGRAF <@t> childrens.com (LINDA MARGRAF) Date: Mon Oct 29 15:45:40 2007 Subject: [Histonet] Job in St Petersburg, FL Message-ID: <4725FFF5020000DA00017018@CNET3.CHILDRENS.COM> Here's a message Kathy was having trouble posting to the list..... We are looking for a Histotech here in St. Petersburg, Florida Position needed in Histology Laboratory performing routine Histology, IHC, Special Stains, along with high tech testing in Electron Microscopy, Immunofluorescence, and InSitu Hybridization. Experience in routine histology; willing to train in specialty high tech testing areas. HT/HTL (ASCP); and Florida Licensure required. ALL CHILDREN'S HOSPITAL Please APPLY ONLINE: www.allkids.org Thank you, Kathy carneyk@allkids.org From Shawnster73 <@t> aol.com Mon Oct 29 16:51:15 2007 From: Shawnster73 <@t> aol.com (Shawnster73@aol.com) Date: Mon Oct 29 16:51:30 2007 Subject: [Histonet] Pathology the Movie Message-ID: I thought you all might get a kick out of this!! I know I did. _www.enterpathologylab.com_ (http://www.enterpathologylab.com) Michelle ************************************** See what's new at http://www.aol.com From katherine-walters <@t> uiowa.edu Mon Oct 29 16:52:02 2007 From: katherine-walters <@t> uiowa.edu (Walters, Katherine S) Date: Mon Oct 29 16:52:07 2007 Subject: [Histonet] sirius red F3B Message-ID: Dear Colleagues, I just want to clarify this issue. IS there such a thing as Sirius red F3B (C.I. 35782)? I have tried to find a source to buy it and have only been able to find stains without a C.I. # or a C.I. # of 35780. When I Goggle this stain, the following sentence appears verbatim over and over: Collagen is birefringent when stained with Sirius red F3B (C.I. 35782). Is this a typo that just gets repeated? Is it a stain that is no longer manufactured? Does anyone know? Katherine Walters Histology Director Central Microscopy Research Facility University of Iowa 85 Eckstein Medical Research Building Iowa City, Iowa 52242-1101 phone: (319) 335-8142 fax: (319) 384-4469 katherine-walters@uiowa.edu From AGrobe2555 <@t> aol.com Mon Oct 29 16:54:00 2007 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Mon Oct 29 16:54:09 2007 Subject: [Histonet] Pig vs Sheep cell marker? Message-ID: Good afternoon, After a bit of searching, it figured I would ask this question here. Do any of you know of an antibody/marker that can be used to differentiate sheep from pig cells in fixed tissues/mixed cultures? A cell-surface marker/intracellular protein......? This is a second-hand question, so I don't know the specifics of the experimental system yet...... Thanks in advance, Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital ************************************** See what's new at http://www.aol.com From JWEEMS <@t> sjha.org Mon Oct 29 17:01:30 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Mon Oct 29 17:01:54 2007 Subject: [Histonet] We need a report from NSH Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F406F@sjhaexc02.sjha.org> for the had to stay home because the new computer system was supposed to go-live on Oct 28 but now has been reset to Dec. 1 and who knows when it will really happen kind of folks... That would be me....j:>) Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From jacobc <@t> mmc.org Mon Oct 29 17:08:37 2007 From: jacobc <@t> mmc.org (Christine Jacobs) Date: Mon Oct 29 17:08:44 2007 Subject: [Histonet] C4d Message-ID: <20071029T180837Z_C07000110000@mmc.org> I was wondering what labs that offer this antibody are doing to get around the fact that the antibody is rated RUO. I recently purchased the Quidel antibody (cat # A213) and was surprised to find the enclosed spec. sheet rate it as an RUO. The spec sheet I downloaded from the internet rated it as an "ASR". Our lab tries to avoid the RUO-rated antibodies as much as possible. The renal clinicians are pushing hard to get this antibody up and running so I am feeling backed into the corner. Any suggestions? CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the use of the intended recipient(s) only and may contain information that is privileged, confidential, and prohibited from unauthorized disclosure under applicable law. If you are not the intended recipient of this message, any dissemination, distribution, or copying of this message is strictly prohibited. If you received this message in error, please notify the sender by reply email and destroy all copies of the original message and attachments. From Shawnster73 <@t> aol.com Mon Oct 29 18:01:23 2007 From: Shawnster73 <@t> aol.com (Shawnster73@aol.com) Date: Mon Oct 29 18:03:26 2007 Subject: [Histonet] sirius red F3B Message-ID: The CI name for Sirius Red F3B is Direct Red 80 and the CI number for Direct Red 80 is 35780. I'm suspicious that once upon a time someone mistyped the CI number and it has carried through to today. I currently use Direct Red 80, Alfa Aesar, B21693-06 purchased from Fisher Scientific (or whatever they are called now). Okay, I know that doesn't really answer your question, but I hope it helps a little. PS I checked StainFile (_http://stainsfile.info/StainsFile/jindex.html_ (http://stainsfile.info/StainsFile/jindex.html) ) out of curiosity and didn't find any stain with that CI number either. Michelle NIBRI, Cambridge In a message dated 10/29/2007 5:52:34 P.M. Eastern Daylight Time, katherine-walters@uiowa.edu writes: Dear Colleagues, I just want to clarify this issue. IS there such a thing as Sirius red F3B (C.I. 35782)? I have tried to find a source to buy it and have only been able to find stains without a C.I. # or a C.I. # of 35780. When I Goggle this stain, the following sentence appears verbatim over and over: Collagen is birefringent when stained with Sirius red F3B (C.I. 35782). Is this a typo that just gets repeated? Is it a stain that is no longer manufactured? Does anyone know? Katherine Walters Histology Director Central Microscopy Research Facility University of Iowa 85 Eckstein Medical Research Building Iowa City, Iowa 52242-1101 phone: (319) 335-8142 fax: (319) 384-4469 katherine-walters@uiowa.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************** See what's new at http://www.aol.com From llewllew <@t> shaw.ca Mon Oct 29 18:07:06 2007 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Mon Oct 29 18:07:54 2007 Subject: [Histonet] sirius red F3B References: Message-ID: <002501c81a80$6d01cdf0$05024246@yourlk4rlmsu> Yes, it is a typo that keeps being repeated. Sirius red F3B is CI# 35780 or Direct red 80. Bryan Llewellyn ----- Original Message ----- From: "Walters, Katherine S" To: Cc: Sent: Monday, October 29, 2007 2:52 PM Subject: [Histonet] sirius red F3B Dear Colleagues, I just want to clarify this issue. IS there such a thing as Sirius red F3B (C.I. 35782)? I have tried to find a source to buy it and have only been able to find stains without a C.I. # or a C.I. # of 35780. When I Goggle this stain, the following sentence appears verbatim over and over: Collagen is birefringent when stained with Sirius red F3B (C.I. 35782). Is this a typo that just gets repeated? Is it a stain that is no longer manufactured? Does anyone know? Katherine Walters Histology Director Central Microscopy Research Facility University of Iowa 85 Eckstein Medical Research Building Iowa City, Iowa 52242-1101 phone: (319) 335-8142 fax: (319) 384-4469 katherine-walters@uiowa.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From s.glyn-jones <@t> auckland.ac.nz Mon Oct 29 22:37:02 2007 From: s.glyn-jones <@t> auckland.ac.nz (Sarah Glyn-Jones) Date: Mon Oct 29 22:37:20 2007 Subject: [Histonet] Timms copper stain Message-ID: <007e01c81aa6$225fdbe0$5abad882@sfac.auckland.ac.nz> Hi, I have recently begun trying to optimise Timms copper stain on kidney tissue. I have frozen tissue sections and I leave them in 1% sodium sulphide for 1hr before TCA treatment and then the Timms reagent. The recipe I am using is: Solution A 5% silver nitrate aqueous (5g in 100ml distilled H2O). Solution B Hydroquinone 2.0gms Citric acid (monohydrate) 5.0gms Distilled water 100ml Develop sections in freshly filtered solution of 1 part A and 5 parts B for 5min There are a number of different recipes for the Timms reagent and the one I am currently using is not working. I cannot seem to get the stain into the tissue. I end up with lots of staining on top of the tissue despite heavy washing. Does anyone have a different recipe that I could try? I have seen recipes for Timms reagent using gum arabic and 10% hydroquinone, except hydroquinone is only soluble in water to 7% (I tried making a 10% solution and it was a disaster). Any suggestions would be greatly appreciated. Thanks! Sarah PhD Candidate Proteomics and Biomedicine Group School of Biological Sciences Thomas Building University of Auckland Tel: (09) 373-7599 ext 85763 From Kemlo.Rogerson <@t> waht.swest.nhs.uk Tue Oct 30 02:45:39 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Oct 30 02:45:44 2007 Subject: [Histonet] curious about soaking paraffin blocks. Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222F069@wahtntex2.waht.swest.nhs.uk> Hockey in Canada is different from field hockey in the UK. Here it is a blood sport. We expect teeth to be knocked out at every game. Bryan Still played by girls? I'm impressed the girls in the UK (despite popular myth) look after their teeth. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; If someone listens, or stretches out a hand, or whispers a kind word of encouragement, or attempts to understand a lonely person, extraordinary things begin to happen. --Loretta Girzaitis This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From gu.lang <@t> gmx.at Tue Oct 30 04:20:03 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Oct 30 04:20:16 2007 Subject: AW: [Histonet] Pathology the Movie In-Reply-To: Message-ID: <001c01c81ad6$0e8cef60$0202fea9@dielangs.at> Igitigit! That's to much for my thin nerves. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Shawnster73@aol.com Gesendet: Montag, 29. Oktober 2007 22:51 An: Histonet@lists.utsouthwestern.edu Betreff: [Histonet] Pathology the Movie I thought you all might get a kick out of this!! I know I did. _www.enterpathologylab.com_ (http://www.enterpathologylab.com) Michelle ************************************** See what's new at http://www.aol.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From n.cragg <@t> epistem.co.uk Tue Oct 30 06:00:13 2007 From: n.cragg <@t> epistem.co.uk (Nicola Cragg) Date: Tue Oct 30 06:00:33 2007 Subject: [Histonet] Looking for an manual for an old Microm cryostat Message-ID: Dear All, We have just acquired an old Microm cryostat (Heidelberg type 500 OM 124.557 CIBA-GEIGY Basel) which looks in quite good condition and it has a motorised pedal which seems to work but I have no manual. I have tried to contact Microm but with no joy yet! I can't wait to have a go on it (as despite it being so old I've been asking for a cryostat for the last 5 years and beggars can't be chosers), but there are a couple of things I'm a bit worried about - the handwheel doesn't seem to have a brake on it and there is a flexible arm with a rough-sided metal block attached to it that seems to just flop into the middle of the chamber and I don't know whatit's for!! I've only ever used Leica cryostats before. Anybody got any ideas aor better still a copy of the manual? Thanks, Nicola This message has been scanned for viruses by BlackSpider MailControl - www.blackspider.com From LSebree <@t> uwhealth.org Tue Oct 30 07:46:46 2007 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Tue Oct 30 07:46:52 2007 Subject: [Histonet] C4d In-Reply-To: <20071029T180837Z_C07000110000@mmc.org> Message-ID: We put a disclaimer on our reports that have used RUO antibodies to achieve a diagnosis. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Christine Jacobs Sent: Monday, October 29, 2007 5:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] C4d I was wondering what labs that offer this antibody are doing to get around the fact that the antibody is rated RUO. I recently purchased the Quidel antibody (cat # A213) and was surprised to find the enclosed spec. sheet rate it as an RUO. The spec sheet I downloaded from the internet rated it as an "ASR". Our lab tries to avoid the RUO-rated antibodies as much as possible. The renal clinicians are pushing hard to get this antibody up and running so I am feeling backed into the corner. Any suggestions? CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the use of the intended recipient(s) only and may contain information that is privileged, confidential, and prohibited from unauthorized disclosure under applicable law. If you are not the intended recipient of this message, any dissemination, distribution, or copying of this message is strictly prohibited. If you received this message in error, please notify the sender by reply email and destroy all copies of the original message and attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Oct 30 07:55:18 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Oct 30 07:55:22 2007 Subject: [Histonet] Pathology the Movie In-Reply-To: Message-ID: <809562.1083.qm@web61212.mail.yahoo.com> I wonder how many of those watching this movie are going to flee running from any of us when they hear: "I work in Pathology". Ren? J. Shawnster73@aol.com wrote: I thought you all might get a kick out of this!! I know I did. _www.enterpathologylab.com_ (http://www.enterpathologylab.com) Michelle ************************************** See what's new at http://www.aol.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From Kemlo.Rogerson <@t> waht.swest.nhs.uk Tue Oct 30 08:12:28 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Oct 30 08:12:35 2007 Subject: [Histonet] Pathology the Movie Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222F07B@wahtntex2.waht.swest.nhs.uk> Work? Don't you mean fornicate in Pathology? Heady mix of dead bodies, sex and sharp implements, very strange....... Just popped into my Mortuary to make sure the male and female APTs weren't up to something. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; If someone listens, or stretches out a hand, or whispers a kind word of encouragement, or attempts to understand a lonely person, extraordinary things begin to happen. --Loretta Girzaitis This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From sheila_adey <@t> hotmail.com Tue Oct 30 08:35:49 2007 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Tue Oct 30 08:35:54 2007 Subject: [Histonet] (no subject) Message-ID: Good morning netters, I'm asking this question on behalf of our morgue tech. He wants to know if hospitals are paying a weekend on call wage and if so, how much? Thanks in advanceSheila Adey HT MLTPort Huron HospitalMichigan _________________________________________________________________ Send a smile, make someone laugh, have some fun! Start now! http://www.freemessengeremoticons.ca/?icid=EMENCA122 From rjbuesa <@t> yahoo.com Tue Oct 30 09:02:24 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Oct 30 09:02:31 2007 Subject: [Histonet] (no subject) In-Reply-To: Message-ID: <447049.26969.qm@web61212.mail.yahoo.com> Yes, they pay. How much depends on the hospital, and more important, how your friend is able to negotiate. He should evaluate the NEED of the hospital and appreciate his contribution and ask accordingly. Salaries in histology are a real hodgepodge that can vary between hospitals "accross the street". Let your friend negotiate for what he thinks is fair and for what he is willing to accept to meet the hospital needs. If it is NOT unreasonable, they will pay him. Ren? J. sheila adey wrote: Good morning netters, I'm asking this question on behalf of our morgue tech. He wants to know if hospitals are paying a weekend on call wage and if so, how much? Thanks in advanceSheila Adey HT MLTPort Huron HospitalMichigan _________________________________________________________________ Send a smile, make someone laugh, have some fun! Start now! http://www.freemessengeremoticons.ca/?icid=EMENCA122_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From mcauliff <@t> umdnj.edu Tue Oct 30 09:20:32 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Oct 30 09:21:08 2007 Subject: [Histonet] mouse bones cleaned In-Reply-To: <4850136AECAE5447B2E7FF80CA9225300C34AA@UABEXMB4.ad.uab.edu> References: <4850136AECAE5447B2E7FF80CA9225300C34AA@UABEXMB4.ad.uab.edu> Message-ID: <47273DB0.5070002@umdnj.edu> Patricia F Lott wrote: > I have some mouse bones which have been cleaned by dermestid beetles, > then placed into bleach for whitening, and stored in alcohol. Is there > a way to make them last longer? I'm afraid if I just dry them now, they > will be very fragile! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Look into "plastination". Just google the word to learn more. Geoff -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From LMason <@t> wyeth.com Tue Oct 30 09:22:17 2007 From: LMason <@t> wyeth.com (Lawrence Mason) Date: Tue Oct 30 09:22:45 2007 Subject: [Histonet] Staining tissue in Embed 812 resin with Hematoxylin and Eosin? Message-ID: <472705D9020000DB00001B4F@CE08A07M.ad.us.pri.wyeth.com> I have some archived mouse tissues in Embed 812 resin. Is it possible to stain these tissues with hematoxylin and eosin? I was unable to find any relevant info in the archives. Thanks, Larry Mason Wyeth Research Cambridge, MA From n.cragg <@t> epistem.co.uk Tue Oct 30 09:23:48 2007 From: n.cragg <@t> epistem.co.uk (Nicola Cragg) Date: Tue Oct 30 09:24:49 2007 Subject: [Histonet] Soaking paraffin blocks Message-ID: Hi Kemlo, Thanks for the message and explanations - sorry for the slow response - life is very hectic in contract research particularly in a relatively new biotech company - I don't recommend it. The company arose out of the Department of Epithelial Biology at the Paterson Institute for Cancer Research at Christies Hospital and much of the company's work / assays are based on the Prof. Potten's work on the gut (the website will tell you much more). I'm ok with the poor fixation. The majority of our gut samples are fixed in Carnoy's (contains 60% absolute alcohol)for 1 hour and then transferred to 70% alcohol prior to processing. This method has been used for about 30 years, way before my time. What do you mean when you say "If the processing fluids are left on too long, tissue goes hard" - are you referring to too much dehydration or that the fluids have been left on the processor too long? After a previous discussion on Histonet, I had understood that some think it's not possible to over dehydrate - do you not agree? The schedule I use is quite lengthy so I don't think it can be a case of too little dehydration. With the xylene and wax stages, I thought I had encorporated sufficiently long incubation times to ensure clearing and wax penetration of tissues. I have wondered whether my timings are too long and have cut back on the wax times, which has made no difference to the sectioning. Plus I regularly change my reagents and probably do this more than necessary, so I don't think it's too much carry over. I will think more about the schedule though. I do think the nature of the samples has a lot to do with it and as I mentioned before an average block can contain up 96 pieces of intestine orientated on end and it seems to me that cutting through all those different layers of muscle, mucosa and epithelium repeatedly through the block could be the root of the difficulties - although I can cut them fine after a few minutes on wetted ice. It's been an eye-opening thread and it's this kind of thread which challenges the routine methods you do every day without thinking which I think help one become a better histologist, well hopefully anyway!! Thanks, Nicola -----Original Message----- From: Kemlo Rogerson [mailto:Kemlo.Rogerson@waht.swest.nhs.uk] Sent: 29 October 2007 13:40 To: Nicola Cragg; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Soaking paraffin blocks "What am I concerned with is the claim of poor processing - how does one know that it is poor processing, particularly if you haven't seen the blocks or attempted to cut them? What is poor about it - insufficient wax penetration, lack of dehydration? Processing is essentially quite a straight forward process and apart from varying timings in alcohols, xylene and wax, I can't understand how it could go so wrong?!! I have a Leica TP1020 without vacuum and run overnight schedule based on what I've read up - reagents are changed regularly always erring on the side of caution. I am a self-taught histologist (and there may lay your accusations - LOL) and have been "practicing" for over 5 years now, but I have only learnt by reading up on what the experts say and do (including Histonet - which I have found to be a fantastic tutor) and by practice. I am always keen to learn from my more experienced peers so please expand on pharses such as "poor processing" and "poor fixation"." Nicola Poor fixation could mean not enough or too much fixation. Some fixatives harden tissue when 'overexposed' some fixative take some time to penetrate and to fix. Bluntly, IMHO, you can't process properly unless you've fixed 'properly'. Fixation puts the tissue in the correct state to resist the effects of processing and to allow the fluids to penetrate. If the processing fluids are left on too long, tissue goes hard, too little and water or alcohol isn't removed. If the tissue isn't clear in xylene then it's not dehydrated, if it's not dehydrated then you won't get the wax in properly, if you don't get the xylene out then you make the wax and the tissue 'soggy'. Overprocess and you harden the tissue (usually the heat from the wax) and you have to use reclamation techniques such as ice/ water IMHO. You build a house from the foundations up; fixation is your foundation without which nothing underpins the processing. What does your Company do in the UK? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Never does nature say one thing and wisdom another. --Juvenal This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation This message has been scanned for viruses by BlackSpider MailControl - www.blackspider.com From mcauliff <@t> umdnj.edu Tue Oct 30 09:46:10 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Oct 30 09:46:29 2007 Subject: [Histonet] Staining tissue in Embed 812 resin with Hematoxylin and Eosin? In-Reply-To: <472705D9020000DB00001B4F@CE08A07M.ad.us.pri.wyeth.com> References: <472705D9020000DB00001B4F@CE08A07M.ad.us.pri.wyeth.com> Message-ID: <472743B2.7020908@umdnj.edu> Hi Larry: There is, in my opinion, no easy or good, way to do this. If there were, it would probably be in the archives. There were a lot of methods papers published in the late 1960's and into the early 1980's on various ways to get and H&E picture of glut. fixed, osmicated, plastic embedded tissue. The few I looked into seemed like way more trouble than the results demonstrated. Geoff Lawrence Mason wrote: > I have some archived mouse tissues in Embed 812 resin. Is it possible to stain these tissues with hematoxylin and eosin? I was unable to find any relevant info in the archives. > > Thanks, > Larry Mason > Wyeth Research > Cambridge, MA > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From Kemlo.Rogerson <@t> waht.swest.nhs.uk Tue Oct 30 10:08:20 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Oct 30 10:08:25 2007 Subject: [Histonet] Soaking paraffin blocks Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222F07E@wahtntex2.waht.swest.nhs.uk> "What do you mean when you say "If the processing fluids are left on too long, tissue goes hard" - are you referring to too much dehydration or that the fluids have been left on the processor too long? After a previous discussion on Histonet, I had understood that some think it's not possible to over dehydrate - do you not agree? The schedule I use is quite lengthy so I don't think it can be a case of too little dehydration." Nicola Interesting point and one I've thought about before; alcohol is a fixative as well as a dehydrating agent. If you put unfixed tissue on to a processing machine then you logically get alcohol fixation and that classically can make tissue 'hard'. If you put fixed tissue onto a processing machine then I'm assuming that initial fixation 'protects' the tissue against the effects of the alcohol. I agree you can't over dehydrate tissue as, as they say in the Sales, 'once it's gone, its gone'. I do think however that you can overfix tissue and I assume that the longer you leave formalin fixed tissue (a 'soft' reversible fixative) in alcohol then the greater effect that will have as a fixative and then harden. Interestingly Carnoy's is just that, an alcoholic fixative, containing alcohol, chloroform and acetic acid (60% ethyl alcohol). It classically is used for rapid fixation of thin pieces of tissue for relatively short periods of time. It's said to cause considerable shrinkage, dissolves acid soluble cell granules and pigments. It would not be the fixative of choice for the work you do IMHO and I would suggest at least replacing ethanol with methanol but moving to a formalin based fixative may work better. Large blocks would have to remain in the fixative for a long time (alcohol penetrates slowly) so by the time the middle is done, the outside is like a brick. You are using 'wetted ice' I think to reclaim tissue made hard by using a rather harsh fixative known to harden tissue. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; No snowflake in an avalanche ever feels responsible. --George Burns This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From n.cragg <@t> epistem.co.uk Tue Oct 30 10:36:59 2007 From: n.cragg <@t> epistem.co.uk (Nicola Cragg) Date: Tue Oct 30 10:39:46 2007 Subject: [Histonet] Soaking paraffin blocks Message-ID: Hi Kemlo, The reason that we use Carnoy's fixative is that the morphology on H&E is superior to Formalin and we perform a lot of manual histometric analyses by light microscopy, which in particular includes the identification of mitotic and apoptotic cells - these are much easier to recognise in Carnoy's fixed tissue. Also this method has a 30 year history so I don't think I would be able to convince people to change the fixative now!! We do use formalin for other work, i.e. IHC. Nicola -----Original Message----- From: Kemlo Rogerson [mailto:Kemlo.Rogerson@waht.swest.nhs.uk] Sent: 30 October 2007 15:08 To: Nicola Cragg Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Soaking paraffin blocks "What do you mean when you say "If the processing fluids are left on too long, tissue goes hard" - are you referring to too much dehydration or that the fluids have been left on the processor too long? After a previous discussion on Histonet, I had understood that some think it's not possible to over dehydrate - do you not agree? The schedule I use is quite lengthy so I don't think it can be a case of too little dehydration." Nicola Interesting point and one I've thought about before; alcohol is a fixative as well as a dehydrating agent. If you put unfixed tissue on to a processing machine then you logically get alcohol fixation and that classically can make tissue 'hard'. If you put fixed tissue onto a processing machine then I'm assuming that initial fixation 'protects' the tissue against the effects of the alcohol. I agree you can't over dehydrate tissue as, as they say in the Sales, 'once it's gone, its gone'. I do think however that you can overfix tissue and I assume that the longer you leave formalin fixed tissue (a 'soft' reversible fixative) in alcohol then the greater effect that will have as a fixative and then harden. Interestingly Carnoy's is just that, an alcoholic fixative, containing alcohol, chloroform and acetic acid (60% ethyl alcohol). It classically is used for rapid fixation of thin pieces of tissue for relatively short periods of time. It's said to cause considerable shrinkage, dissolves acid soluble cell granules and pigments. It would not be the fixative of choice for the work you do IMHO and I would suggest at least replacing ethanol with methanol but moving to a formalin based fixative may work better. Large blocks would have to remain in the fixative for a long time (alcohol penetrates slowly) so by the time the middle is done, the outside is like a brick. You are using 'wetted ice' I think to reclaim tissue made hard by using a rather harsh fixative known to harden tissue. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; No snowflake in an avalanche ever feels responsible. --George Burns This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation This message has been scanned for viruses by BlackSpider MailControl - www.blackspider.com From jkiernan <@t> uwo.ca Tue Oct 30 12:32:20 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Tue Oct 30 12:32:38 2007 Subject: [Histonet] Timms copper stain Message-ID: Your recipe for the developer does not include a protective colloid, so the mixture will be unstable. Try the developer of Danscher & Zimmer (1978) Histochemistry 55: 27-40. The first three ingredients of the developer may be mixed several hours in advance, but the last (AgNO3) is added immediately before using. 50% (w/v) aqueous gum arabic stock solution: 60 ml; Citrate buffer: 10 ml; Hydroquinone (5.67% w/v, aqueous): 30 ml. Silver nitrate (17% w/v, aqueous stock solution): 0.5 ml. The buffer is: Citric acid (monohydrate) 25.5g, Sodium citrate (dihydrate) 23.5g, dissolved in water and made up to 100 ml. The pH should be 3.7 if an adjustment is necessary use citric acid or diulute NaOH; not hydrochloric acid, which would precipitate the silver out of the developer. Remember that Timm's and related sulphide-silver methods are not specific for copper. They detect any metal with an insoluble sulphide, including iron, zinc and many unphysiological metals that might be present in tissues, such as mercury or lead. If an alcoholic fixative is use (eg hydrogen sulphide dissolved in ethanol) these methods will also detect calcium, because CaS is insoluble in alcohol. There are histochemical methods for copper that are quite specific, though less sensitive than Timm's technique. The specific methods detect abnormal deposits of Cu (as in wilson's disease) but not the copper in normal mammalian tissues. Unless your kidney tissue contains much more copper than zinc, the silver deposits that you see in the sections will not mean anything. For more information see the Danscher & Zimmer paper cited above; also more recent publications by Danscher. The method can be tweaked to make it fairly specific for zinc or mercury. I don't think this can be done for copper, but could be mistaken. John Kiernan Anatomy, UWO London, Canada ----- Original Message ----- From: Sarah Glyn-Jones Date: Monday, October 29, 2007 23:39 Subject: [Histonet] Timms copper stain To: histonet@lists.utsouthwestern.edu > Hi, > > I have recently begun trying to optimise Timms copper stain on kidney > tissue. I have frozen tissue sections and I leave them in 1% > sodium sulphide > for 1hr before TCA treatment and then the Timms reagent. > > The recipe I am using is: > > Solution A > > 5% silver nitrate aqueous (5g in 100ml distilled H2O). > > Solution B > > Hydroquinone 2.0gms > Citric acid > (monohydrate) 5.0gms > Distilled > water 100ml > > Develop sections in freshly filtered solution of 1 part A and 5 > parts B for > 5min > > There are a number of different recipes for the Timms reagent > and the one I > am currently using is not working. I cannot seem to get the > stain into the > tissue. I end up with lots of staining on top of the tissue > despite heavy > washing. > > Does anyone have a different recipe that I could try? I have > seen recipes > for Timms reagent using gum arabic and 10% hydroquinone, except > hydroquinoneis only soluble in water to 7% (I tried making a 10% > solution and it was a > disaster). > > Any suggestions would be greatly appreciated. > > Thanks! > > Sarah > > PhD Candidate > Proteomics and Biomedicine Group > School of Biological Sciences > Thomas Building > University of Auckland > Tel: (09) 373-7599 ext 85763 From CIngles <@t> uwhealth.org Tue Oct 30 12:55:01 2007 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Tue Oct 30 12:55:06 2007 Subject: [Histonet] Pathology the Movie References: Message-ID: <08A0A863637F1349BBFD83A96B27A50A120094@uwhis-xchng3.uwhis.hosp.wisc.edu> As if CSI, etc. weren't bad enough! Oh, and all the dead bodies/sex/sharp objects in the same room? Just remember they're Med Students! They all think they're Gods anyway, with a few notable exceptions (that won't be noted here). Geez but those 'Heroes' actors are sure getting around fast. I believe I heard the guy that plays Sylar is going to be in the next Star Trek movie. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Shawnster73@aol.com Sent: Mon 10/29/2007 4:51 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathology the Movie I thought you all might get a kick out of this!! I know I did. _www.enterpathologylab.com_ (http://www.enterpathologylab.com ) Michelle ************************************** See what's new at http://www.aol.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From froyer <@t> bitstream.net Tue Oct 30 13:26:57 2007 From: froyer <@t> bitstream.net (Ford Royer) Date: Tue Oct 30 13:27:36 2007 Subject: OFF TOPIC RE: [Histonet] Pathology the Movie In-Reply-To: <08A0A863637F1349BBFD83A96B27A50A120094@uwhis-xchng3.uwhis.hosp.wisc.edu> References: <08A0A863637F1349BBFD83A96B27A50A120094@uwhis-xchng3.uwhis.hosp.wisc.edu> Message-ID: <006501c81b22$74c5feb0$7701a80a@Ford> This (Pathology) reminds me of a stand-up routine that Dennis Miller did a number of years ago... and I've never forgotten it. He was talking about some of the "easier" professions that are available. One was County Coroner / Medical Examiner / Pathologists "What's so tough about this? ...surgery on dead people. HA! What's the worst that could happen? ...what, you might get a pulse?!" ;-) Ford M. Royer, MT(ASCP) Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL:? 612-839-1046 Phone:? 763-542-8725 Fax:? 763-546-4830 eMail:? clinicallab@minnesotamedical.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Tuesday, October 30, 2007 12:55 PM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Pathology the Movie As if CSI, etc. weren't bad enough! Oh, and all the dead bodies/sex/sharp objects in the same room? Just remember they're Med Students! They all think they're Gods anyway, with a few notable exceptions (that won't be noted here). Geez but those 'Heroes' actors are sure getting around fast. I believe I heard the guy that plays Sylar is going to be in the next Star Trek movie. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Shawnster73@aol.com Sent: Mon 10/29/2007 4:51 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathology the Movie I thought you all might get a kick out of this!! I know I did. _www.enterpathologylab.com_ (http://www.enterpathologylab.com ) Michelle ************************************** See what's new at http://www.aol.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terena.Few <@t> propathlab.com Tue Oct 30 15:31:38 2007 From: Terena.Few <@t> propathlab.com (Terena Few) Date: Tue Oct 30 15:32:13 2007 Subject: [Histonet] (no subject) Message-ID: Do anyone who do autopsies have any real pictures of an autopsy? It seems interesting. ______________________________________________________________________________ This e-mail may contain confidential or privileged information. If you think you have received this e-mail in error, please advise the sender by reply e-mail and then delete this e-mail immediately. From victor <@t> pathology.washington.edu Tue Oct 30 16:42:09 2007 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Tue Oct 30 16:42:15 2007 Subject: [Histonet] New Thermo Cassette Labeler Message-ID: <4727A531.8000301@pathology.washington.edu> Anyone returning from NSH, did you see the new cassette labeler from Thermo, I believe it is called the PrintMate? I would like to hear your initial reactions to the product. It was supposed to have come out months ago and wondering if the hype has been worth the wait. Thanks for any input. Victor -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From ccpath <@t> gmail.com Tue Oct 30 16:52:31 2007 From: ccpath <@t> gmail.com (j k) Date: Tue Oct 30 16:52:38 2007 Subject: [Histonet] tissue highlighting for embedding/cutting Message-ID: Sorry I missed this when it came up recently. What do people use to highlight tissue to assist in the embedding and cutting process? What to add to formalin bath? Eosin? Safranin O? Others? Do these affect the H&E staining quality in any way? Do these affect immunoperoxidase staining? Do these gunk up the innards of the tissue processor in any way? jim keller From mariakatleba <@t> aol.com Tue Oct 30 17:54:32 2007 From: mariakatleba <@t> aol.com (mariakatleba@aol.com) Date: Tue Oct 30 17:54:40 2007 Subject: [Histonet] pathology assistant job description Message-ID: <8C9E95EF85EC243-6F4-171B@MBLK-M37.sysops.aol.com> Anyone have a PATHOLOGY ASSISTANT job description??? I am working on my employee files and need a "real" job description. My employee? is a path asst. Thanks in advance Maria Katleba MS ?HT (ASCP) Pathology Dept Manager Queen of the Valley Medical Center Napa CA 94558 Fax 707-257-4133 ________________________________________________________________________ Email and AIM finally together. You've gotta check out free AOL Mail! - http://mail.aol.com From sheila_adey <@t> hotmail.com Tue Oct 30 19:25:33 2007 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Tue Oct 30 19:25:38 2007 Subject: [Histonet] tissue highlighting for embedding/cutting In-Reply-To: References: Message-ID: We add approx. 40 ml of our Alcoholic Eosin to the first 100% alcohol on the processor. Works great.Sheila Adey HT MLTPort Huron HospitalMichigan> Date: Tue, 30 Oct 2007 17:52:31 -0400> From: ccpath@gmail.com> To: histonet@lists.utsouthwestern.edu> Subject: [Histonet] tissue highlighting for embedding/cutting> > Sorry I missed this when it came up recently.> What do people use to highlight tissue to assist in the embedding and> cutting process?> What to add to formalin bath? Eosin? Safranin O? Others?> Do these affect the H&E staining quality in any way? Do these affect> immunoperoxidase staining? Do these gunk up the innards of the tissue> processor in any way?> jim keller> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ R U Ready for Windows Live Messenger Beta 8.5? Try it today! http://entertainment.sympatico.msn.ca/WindowsLiveMessenger From JMacDonald <@t> mtsac.edu Wed Oct 31 00:48:52 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Oct 31 00:48:13 2007 Subject: [Histonet] paraffin and microwaves Message-ID: How does one heat paraffin for microwave processing if paraffin i microwave transparent? The protocols that I have read simply say par affin at 84 degrees C or such. They don't say how to get there. Thank you, Jennifer&nb Director, Histotechnician Training ProgramMt. San Antonio College 1100 N. Grand Av Walnut, CA 91789 (909) 594-5611 ext. 4884 [1]jmacdonald@mtsa References 1. 3D"mailto:jmacdonald@mtsac.edu" From Kemlo.Rogerson <@t> waht.swest.nhs.uk Wed Oct 31 02:55:45 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Oct 31 02:55:52 2007 Subject: [Histonet] (no subject) Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222F086@wahtntex2.waht.swest.nhs.uk> "Do anyone who do autopsies have any real pictures of an autopsy? It seems interesting. Terena" In the UK that would break so many laws and maybe place the photographer in the hands of the Police that I hope no-one answers. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; No snowflake in an avalanche ever feels responsible. --George Burns This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From rjbuesa <@t> yahoo.com Wed Oct 31 07:44:40 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Oct 31 07:44:45 2007 Subject: [Histonet] paraffin and microwaves In-Reply-To: Message-ID: <966941.32869.qm@web61216.mail.yahoo.com> Jennifer: You cannot heat paraffin in a microwave oven because, as you point out, paraffin is microwave transparent, i.e. is not a molecule with areas that can absorb the MW energy. Paraffin heating in MW oven is possible when the paraffin itself contains aditives that can absorb the energy, or when fragments of Weflon (=Teflon covered with carbon that abosorbs MW) is added to the container either is constructed with Weflon or has a piece of Weflon in the bottom (that sometimes rotates). Another solution is how Sakura has developed its Xpress TP, with 4 chambers, 2 heated by MW of low intensity, and 2 regular chambers, heated by convection where the paraffin infiltration takes place. At the beginning the addition of small marble or earthware balls in the paraffin bath was a solution. The MW heated the marbles and the paraffin was heated by heat transmission. Also, if you heat the paraffin in a conventional oven and add the cassettes and place them both in the MW oven, the paraffin will remain melted and the cassettes with their tissues inside will act like the marbles and will irradiate the heat they receive from the MW. Ren? J. Jennifer MacDonald wrote: How does one heat paraffin for microwave processing if paraffin i= s microwave transparent? The protocols that I have read simply say par affin at 84 degrees C or such. They don't say how to get there. Thank you, Jennifer&nb= sp;MacDonald Director, Histotechnician Training ProgramMt. San Antonio College 1100 N. Grand Av= e. Walnut, CA 91789 (909) 594-5611 ext. 4884 [1]jmacdonald@mtsa= c.edu References 1. 3D"mailto:jmacdonald@mtsac.edu" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From nyilmaz <@t> mersin.edu.tr Wed Oct 31 08:06:59 2007 From: nyilmaz <@t> mersin.edu.tr (Nejat) Date: Wed Oct 31 08:06:28 2007 Subject: [Histonet] perfusion fixation Message-ID: <000501c81bbe$ebcd5de0$5201a8c0@NEJAT1> Dear Netters, We are searching to buy a suitable pump for perfusion fixation on animals. Is there anybody to share experiences with us? Thanks in advance. Dr. Necat Yilmaz Mersin Universitesi Tip Fakultesi Histoloji ve Embriyoloji Anabilim Dali From mcauliff <@t> umdnj.edu Wed Oct 31 08:22:13 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Oct 31 08:22:29 2007 Subject: [Histonet] (no subject) In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBF0222F086@wahtntex2.waht.swest.nhs.uk> References: <86ADE4EB583CE64799A9924684A0FBBF0222F086@wahtntex2.waht.swest.nhs.uk> Message-ID: <47288185.7000609@umdnj.edu> There was a case in the US last year in which a photographer suffered serious legal difficulties for photographing an autopsy. I think the charges were based on revealing confidential medical information. I don't remember if the person actually went to jail but he was probably out a significant amount in legal fees. Geoff Kemlo Rogerson wrote: > "Do anyone who do autopsies have any real pictures of an autopsy? It > seems interesting. > Terena" > > In the UK that would break so many laws and maybe place the photographer > in the hands of the Police that I hope no-one answers. > > Kemlo Rogerson > Pathology Manager > DD 01934 647057 or extension 3311 > Mob 07749 754194; Pager 07659 597107; > > No snowflake in an avalanche ever feels responsible. --George Burns > > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. > Please inform me that this message has gone astray before deleting it. > Thank you for your co-operation > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From mcauliff <@t> umdnj.edu Wed Oct 31 08:25:44 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Oct 31 08:25:58 2007 Subject: [Histonet] perfusion fixation In-Reply-To: <000501c81bbe$ebcd5de0$5201a8c0@NEJAT1> References: <000501c81bbe$ebcd5de0$5201a8c0@NEJAT1> Message-ID: <47288258.9090200@umdnj.edu> I use a small perfusion pump for rats and mice, nothing fancy. Be sure to use a rate of flow that approximates the rate of circulation of the animal's blood. Also, some recommend extensive washing out of blood, I have found this to be a waste of time. Geoff Nejat wrote: > Dear Netters, > > We are searching to buy a suitable pump for perfusion fixation on > animals. Is there anybody to share experiences with us? > Thanks in advance. > > Dr. Necat Yilmaz > Mersin Universitesi Tip Fakultesi > Histoloji ve Embriyoloji Anabilim Dali > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From Kemlo.Rogerson <@t> waht.swest.nhs.uk Wed Oct 31 08:42:02 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Oct 31 08:42:14 2007 Subject: [Histonet] perfusion fixation Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222F092@wahtntex2.waht.swest.nhs.uk> Why not use the force of gravity; if you put a header tank 1 metre about the animal and introduce the fixative from that isn't that one atmosphere of pressure? Thought it was as that's how I perfused collier's lungs when we doing pneumoconiosis studies for compensation. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; No snowflake in an avalanche ever feels responsible. --George Burns This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From ccross6032 <@t> aol.com Wed Oct 31 08:51:21 2007 From: ccross6032 <@t> aol.com (Cheryl Cross) Date: Wed Oct 31 08:51:39 2007 Subject: [Histonet] perfusion fixation In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBF0222F092@wahtntex2.waht.swest.nhs.uk> References: <86ADE4EB583CE64799A9924684A0FBBF0222F092@wahtntex2.waht.swest.nhs.uk> Message-ID: i agree - with gravity you have little chance of perfusing at too high a pressure; for neurotox/neuropath it makes the brains particularly fix much better. Cheryl Cheryl Cross, DVM, Dipl. ACVP Researcher University Corporation for Atmospheric Research College of Veterinary Medicine University of Tennessee Department of Pathology 2407 River Drive, Room A201 Knoxville, TN 37996-4542 (423) 967-2724 fax: 865-974-5616 ccross@ucar.edu On Oct 31, 2007, at 9:42 AM, Kemlo Rogerson wrote: > Why not use the force of gravity; if you put a header tank 1 metre > about > the animal and introduce the fixative from that isn't that one > atmosphere of pressure? Thought it was as that's how I perfused > collier's lungs when we doing pneumoconiosis studies for compensation. > > Kemlo Rogerson > Pathology Manager > DD 01934 647057 or extension 3311 > Mob 07749 754194; Pager 07659 597107; > No snowflake in an avalanche ever feels responsible. --George Burns > > This e-mail is confidential and privileged. If you are not the > intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in > reliance on > its contents: to do so is strictly prohibited and may be unlawful. > Please inform me that this message has gone astray before deleting it. > Thank you for your co-operation > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Wed Oct 31 10:56:25 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Wed Oct 31 10:56:35 2007 Subject: [Histonet] (no subject) Message-ID: <5C0BED61F529364E86309CADEA63FEF2F3AE1E@TRFT-EX01.xRothGen.nhs.uk> I would be interested to know what laws are being or would be flouted on either side of the pond. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Geoff McAuliffe Sent: 31 October 2007 13:22 To: Kemlo Rogerson Cc: histonet@lists.utsouthwestern.edu; Terena Few Subject: Re: [Histonet] (no subject) There was a case in the US last year in which a photographer suffered serious legal difficulties for photographing an autopsy. I think the charges were based on revealing confidential medical information. I don't remember if the person actually went to jail but he was probably out a significant amount in legal fees. Geoff Kemlo Rogerson wrote: > "Do anyone who do autopsies have any real pictures of an autopsy? It > seems interesting. > Terena" > > In the UK that would break so many laws and maybe place the > photographer in the hands of the Police that I hope no-one answers. > > Kemlo Rogerson > Pathology Manager > DD 01934 647057 or extension 3311 > Mob 07749 754194; Pager 07659 597107; > > No snowflake in an avalanche ever feels responsible. --George Burns > > This e-mail is confidential and privileged. If you are not the > intended recipient please accept my apologies; please do not disclose, > copy or distribute information in this e-mail or take any action in > reliance on its contents: to do so is strictly prohibited and may be unlawful. > Please inform me that this message has gone astray before deleting it. > Thank you for your co-operation > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Wed Oct 31 11:31:29 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Oct 31 11:31:35 2007 Subject: [Histonet] (no subject) Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222F095@wahtntex2.waht.swest.nhs.uk> You are the Lawyer, you tell us. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; No snowflake in an avalanche ever feels responsible. --George Burns This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From drbugge <@t> gmail.com Wed Oct 31 12:59:01 2007 From: drbugge <@t> gmail.com (Dawn Bugge) Date: Wed Oct 31 13:00:30 2007 Subject: [Histonet] Histolotech Opening - Seattle, WA Message-ID: <1c4db3750710311059l2278b698n6f71fbacfc048dee@mail.gmail.com> Histotech position open in a high volume GI lab. Looking for an experienced tech for job duties including: accessioning, grossing, embedding, cutting, coverslipping, etc. We process tissue for a large group of Gastroenterologists so are tissues are very small. Currently we are located near the Northgate Mall in Seattle but are relocating further north possibly in the Mill Creek area. We offer benefits including: 401K, Health, Dental and Vision. If you are interested please email Dawn Bugge at drbugge@gmail.com or call 206-365-1438. -- Dawn R Bugge Seattle Histology a Division of Puget Sound Gastroenterology 206-365-1438 drbugge@gmail.com From Terena.Few <@t> propathlab.com Wed Oct 31 13:06:43 2007 From: Terena.Few <@t> propathlab.com (Terena Few) Date: Wed Oct 31 13:06:40 2007 Subject: [Histonet] RE: no subject In-Reply-To: References: Message-ID: OK ignore the question I asked earlier. I had no idea it is illegal to do that. Im not a lawyer!!!!!!!!! Please ignore the question??!!!!!!!!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, October 31, 2007 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 47, Issue 45 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." _____________________________________________________________________________ _ This e-mail may contain confidential or privileged information. If you think you have received this e-mail in error, please advise the sender by reply e-mail and then delete this e-mail immediately. ______________________________________________________________________________ This e-mail may contain confidential or privileged information. If you think you have received this e-mail in error, please advise the sender by reply e-mail and then delete this e-mail immediately. From JWEEMS <@t> sjha.org Wed Oct 31 13:24:26 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Oct 31 13:25:15 2007 Subject: [Histonet] RE: no subject In-Reply-To: Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F40C6@sjhaexc02.sjha.org> In the US we are now subject to HIPAA privacy laws that are quite strict. Regardless, it can be very traumatic to families if that info gets out. Remember the Dale Earnhardt debacle? At just about that same time, a young man died from a drug overdose here in GA . The police officer or detective - one of the officials - took pictures of the autopsy for his private collection. Later, he allowed his child to use the photos for a class project. Even tho the face was not shown, there was a recognizable tatoo on the shoulder that his friends immediately recognized. The whole school and community was in an uproar - everyone calling his mother. It was so traumatic for everyone who knew him and for his family. His mother worked to have legislation passed that makes it illegal to show photos in GA now. I believe this is happening in several states. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Terena Few Sent: Wednesday, October 31, 2007 2:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: no subject OK ignore the question I asked earlier. I had no idea it is illegal to do that. Im not a lawyer!!!!!!!!! Please ignore the question??!!!!!!!!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, October 31, 2007 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 47, Issue 45 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." _____________________________________________________________________________ _ This e-mail may contain confidential or privileged information. If you think you have received this e-mail in error, please advise the sender by reply e-mail and then delete this e-mail immediately. ______________________________________________________________________________ This e-mail may contain confidential or privileged information. If you think you have received this e-mail in error, please advise the sender by reply e-mail and then delete this e-mail immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From laurie.colbert <@t> huntingtonhospital.com Wed Oct 31 13:34:20 2007 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Wed Oct 31 13:34:26 2007 Subject: [Histonet] Flammable and acid storage Message-ID: <57BE698966D5C54EAE8612E8941D768301EA5FC0@EXCHANGE3.huntingtonhospital.com> Are there any regulations that say flammables and acids cannot be stored together? Laurie Colbert From Barry.R.Rittman <@t> uth.tmc.edu Wed Oct 31 13:38:15 2007 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Wed Oct 31 13:38:20 2007 Subject: [Histonet] Flammable and acid storage In-Reply-To: <57BE698966D5C54EAE8612E8941D768301EA5FC0@EXCHANGE3.huntingtonhospital.com> Message-ID: yes -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Wednesday, October 31, 2007 1:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Flammable and acid storage Are there any regulations that say flammables and acids cannot be stored together? Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AGrobe2555 <@t> aol.com Wed Oct 31 13:39:25 2007 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Wed Oct 31 13:39:35 2007 Subject: [Histonet] Re: Perfusion fixation Message-ID: Rather than using a suspended fluid reservoir, I have used a IV pressure bag dialed into the average systolic pressure of the animal (+/- 10mm Hg). The most annoying part was getting the fixative into an IV bag. Insert a catheter and go.... Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital ************************************** See what's new at http://www.aol.com From Herrick.James <@t> mayo.edu Wed Oct 31 16:42:49 2007 From: Herrick.James <@t> mayo.edu (Herrick, James L.) Date: Wed Oct 31 16:42:59 2007 Subject: [Histonet] Deplastification and immuno question Message-ID: <572057D3BDD52A46BD05BC6DA50686110CAEDF@MSGEBE22.mfad.mfroot.org> Hi all, Has anyone had any success with attaching GMA or MMA embedded sections to glass slides, deplastifying and running immuno, without the sections loosening or falling off of the slide? If so, were you successful with antigen retrieval and antibody attachment? Thank you much for your help. Jim From thisisann <@t> aol.com Wed Oct 31 21:31:28 2007 From: thisisann <@t> aol.com (thisisann@aol.com) Date: Wed Oct 31 21:32:29 2007 Subject: [Histonet] Requisition Imaging Message-ID: <8C9EA4670A3ED52-CD4-24C1@WEBMAIL-MA04.sysops.aol.com> I am employed by a small, but quickly growing, Histology laboratory that is in need of a method of "imaging" requisition slips.? Rather than re-invent the wheel, can anyone suggest a reliable product, currently on the market, that?can meet our needs?? Thank you,? Ann Angelo ________________________________________________________________________ Email and AIM finally together. You've gotta check out free AOL Mail! - http://mail.aol.com