From Kemlo.Rogerson <@t> waht.swest.nhs.uk Tue May 1 01:41:15 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue May 1 01:41:23 2007 Subject: [Histonet] Re: Emperor Message-ID: <86ADE4EB583CE64799A9924684A0FBBF01247623@wahtntex2.waht.swest.nhs.uk> I was just being too clever for my own good, bound to cock it up eventually. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; I want it said of me by those who knew me best, that I always plucked a thistle and planted a flower where I thought a flower would grow. --Abraham Lincoln This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From tim.morken <@t> thermofisher.com Tue May 1 13:22:46 2007 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Tue May 1 13:23:13 2007 Subject: [Histonet] California Society for Histotechnology Spring Meeting May 17-20 Message-ID: <6BFF6D137DF6BC43B33891BA96E83B1959B5DD@PGHCR-EXMB-VS-1.na.fshrnet.com> Information and registration forms for the California Society for Histotechnology Spring Meeting on May 17-20 in San Mateo (near San Jose) can be downloaded at the California society website: www.californiahistology.org Tim Morken From Karen.Heckford <@t> CHW.edu Tue May 1 14:51:36 2007 From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF) Date: Tue May 1 14:51:44 2007 Subject: [Histonet] Cell Button Prep Message-ID: I was wondering if anyone would be so kind as giving input on the different ways of preparing a cell button. We work a lot with Pleural Fluid and Ascites Fluid. I have been told two different ways. One is just 1/2 and 1/2 with fluid and 95%alcohol and spend down decant and get your cell button. The other is spend down the fluid decant add 10%formalin respin and let set then get your cell button. Of course this all after I have made my cytospins. We do not have a ThinPrep type machine. Any input would be very appreciative. I realize there is probably more ways to do this that I have not heard of. Cheers, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathology Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu From pathology.histology <@t> gmail.com Tue May 1 15:24:42 2007 From: pathology.histology <@t> gmail.com (path lab) Date: Tue May 1 15:24:46 2007 Subject: [Histonet] bone marrow smears Message-ID: <73841640705011324w79dfab34u57e8553b37923836@mail.gmail.com> Could anyone please provide a procedure for bone marrow smears from necropsy to staining with Wrights-Giemsa? Thanks for your help in advance. From jjohnson <@t> crispregional.org Tue May 1 15:55:49 2007 From: jjohnson <@t> crispregional.org (Jennifer Johnson) Date: Tue May 1 15:58:39 2007 Subject: [Histonet] Fatty tissue Message-ID: <000701c78c33$1eb04e00$2082010a@main.crispregional.org> Does anyone have any suggestions for cutting better frozen sections of very fatty tissue (breast in particular)? When I turn the cryostat colder, my Pathologist complains about the artifact on the permanent sections. Any help is appreciated. Thanks, Jennifer From Eric <@t> ategra.com Tue May 1 17:18:17 2007 From: Eric <@t> ategra.com (Eric Dye (ext 223)) Date: Tue May 1 17:00:11 2007 Subject: [Histonet] 2 F/T Permanent Histology Bench and Histo Supv positions in SW Texas & East Texas Message-ID: Hi - Histonetters -Are you still working at ? I am presently on a search for several of my clients in Texas who are looking to hire HistoTech employees. I have Two(2) F/T Permanent Histology positions in Texas.BOTH positions are perm F/T as direct employees of my clients. They are regular employee positions that offer you 40hrs/wk with full employe benfits - including: 401-K, Full Health Insur., Vacation/Sick/Holiday & Full Relo. Permanent Histology positions in Texas 1. S.W. Texas - Bench Histo Tech - Mon - Fri Days - work schedule is flexible to meet YOUR needs. 2. East Texas - HistoTech Supervisor - Mon - Fri Days - 8a -5pm 3. East Texas - Bench Histo Tech Supervisor - Mon - Fri Days - hours flex to suit your needs. If you are interested in any of the HISTOTECH J0BS listed above - please call me at 800-466-9919 ext 223 or cell - 407-756-5507...OR .. May I call you at or to discuss these Histo Tech openings? Thank you, Eric Dye-Sr Allied Healthcare Recruiter 800-466-9919 ext 223 or Cell - 407-756-5507 P.S.: Feel free to pass along this email and my phone number to anyone who you think might be interested. P.S.S.: The clients are currently interviewing - and the positions will close soon - so if you are interested, please call me today at 1-800-466-9919 ext 223 --------------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing 800-466-9919 ext 223 - voice 407 671 6075 - fax eric@ategra.com To Learn More About Ategra: http://www.ategra.com ---------------------------------------------------------------- If you received this by mistake, or if you wish not to hear from me, please shoot me a mail to let me know and I'll not mail you again. ---------------------------------------------------------------- ---------------------------------------------------------------- Note: this message is intended for: Histonetters at histonet@lists.utsouthwestern.edu ---------------------------------------------------------------- From conniegrubaugh <@t> hotmail.com Tue May 1 22:19:58 2007 From: conniegrubaugh <@t> hotmail.com (connie grubaugh) Date: Tue May 1 22:20:01 2007 Subject: [Histonet] =?windows-1252?q?peloris=99?= Message-ID: In the market for a new processor and would like feed back on the Peloris good and bad from anyone that has this machine. Connie G. _________________________________________________________________ Discover the new Windows Vista http://search.msn.com/results.aspx?q=windows+vista&mkt=en-US&form=QBRE From WWmn916 <@t> aol.com Tue May 1 22:33:21 2007 From: WWmn916 <@t> aol.com (WWmn916@aol.com) Date: Tue May 1 22:33:29 2007 Subject: [Histonet] Certified thermometers Message-ID: Hello everyone, I'm hoping for a little help. What is everyone using to calibrate routine thermometers in the histology lab (thermometers for refrig, H20 baths, IPOX machines....etc.)? We can no longer use mercury filled certified thermometers to test the others. Any suggestions? How often do the certified thermometers need to be recalibrated? Thanks much, Deb King California ************************************** See what's free at http://www.aol.com. From Kemlo.Rogerson <@t> waht.swest.nhs.uk Wed May 2 02:11:00 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed May 2 02:11:06 2007 Subject: [Histonet] Cell Button Prep Message-ID: <86ADE4EB583CE64799A9924684A0FBBF01247645@wahtntex2.waht.swest.nhs.uk> You could try the hypaque method. If you are interested I could get the method but it bluntly uses a density gradient. You spin down the fluid remove supernatant and reconstitute with saline. You make a solution of hypaque and layer the cell/ saline solution on top. You then spin, rbcs and crude fall through the hypaque and larger cells float on top of the hypaque. Harvest these cells, wash and then mix with agar and cool. Process and cut and stain. Job done. But ThinPrep is the way forward from my experience and if you are clever you can come up with some sedimentation process that might emulate ThinPrep similar to the one SurgiPath was flogging; I think it was French but god knows what happened to it. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; We need to be open to the possibility that colleagues and even strangers have information and perspectives that may be of value to us. --Margaret Wheatley This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From louise.renton <@t> gmail.com Wed May 2 03:38:18 2007 From: louise.renton <@t> gmail.com (louise renton) Date: Wed May 2 03:38:27 2007 Subject: [Histonet] osteoclast/ osteoblast markers Message-ID: Hi all, what recommendations for the most specific immuno markers for osteoclasts, osteoblasts and macrophages in FFPE primate/human tissue? Anybody using CD163 and what have your results been like Thanks in advance best regards -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From Doug.Geddes <@t> lhsc.on.ca Wed May 2 07:04:42 2007 From: Doug.Geddes <@t> lhsc.on.ca (Doug Geddes) Date: Wed May 2 07:05:06 2007 Subject: [Histonet] CD52 Message-ID: <4638461A0200006100001DF6@lhscgwiao.lhsc.on.ca> Looking for a reliable supplier of CD52 for formalin fixed paraffin embedded tissue, and any methods. Doug Geddes BSc, MLT Department of Pathology London Helath Sciences Centre London, ON Canada From raghulvet <@t> yahoo.co.in Wed May 2 08:32:15 2007 From: raghulvet <@t> yahoo.co.in (raghul) Date: Wed May 2 08:32:23 2007 Subject: [Histonet] alcian blue_precipitaion_solution Message-ID: <681959.20532.qm@web8322.mail.in.yahoo.com> dear histonetters hope atleast the persons remember my problem of alcian blue(sigma) precpitating in dis. water. I went on to stirr for 2 hours followed by boiling for 5 min. I could make a 2.5ph solution of alcian blue and its staining well. I think boiling was the main reason. thanks for all those suggestions raghul medclone biotech chennai, india --------------------------------- Check out what you're missing if you're not on Yahoo! Messenger From rjbuesa <@t> yahoo.com Wed May 2 08:40:26 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 2 08:40:30 2007 Subject: [Histonet] Fatty tissue In-Reply-To: <000701c78c33$1eb04e00$2082010a@main.crispregional.org> Message-ID: <885157.50026.qm@web61219.mail.yahoo.com> Deep freeze quickly, cut slowly, increase thickness "just a little bit" and use the "anti-rolling" devise (of a camel hair brush) to "pull" the section. Ren? J. Jennifer Johnson wrote: Does anyone have any suggestions for cutting better frozen sections of very fatty tissue (breast in particular)? When I turn the cryostat colder, my Pathologist complains about the artifact on the permanent sections. Any help is appreciated. Thanks, Jennifer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From asachau <@t> titanmed.com Wed May 2 08:40:32 2007 From: asachau <@t> titanmed.com (April Sachau) Date: Wed May 2 08:41:27 2007 Subject: [Histonet] MOHS In-Reply-To: <681959.20532.qm@web8322.mail.in.yahoo.com> Message-ID: <7E3ACD48BA6E26408F3188FBF08693F78B246F@titansbs1.corp.titanmed.com> Good Morning, Can anyone please tell me why it has been impossible to find someone with strong MOHS experience? April Sachau Titan Medical Group Staff Supervisor Phone (866) 332-9600 Ext. 1023 Fax (402) 332-5181 asachau@titanmed.com see us on the web at www.titanmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of raghul Sent: Wednesday, May 02, 2007 8:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] alcian blue_precipitaion_solution dear histonetters hope atleast the persons remember my problem of alcian blue(sigma) precpitating in dis. water. I went on to stirr for 2 hours followed by boiling for 5 min. I could make a 2.5ph solution of alcian blue and its staining well. I think boiling was the main reason. thanks for all those suggestions raghul medclone biotech chennai, india --------------------------------- Check out what you're missing if you're not on Yahoo! Messenger _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed May 2 08:50:36 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 2 08:51:01 2007 Subject: [Histonet] Certified thermometers In-Reply-To: Message-ID: <872229.17519.qm@web61224.mail.yahoo.com> Calibrate them yourself! Just place your thermometer in boilong water and, if you are at sea level and there is no atmosferic low pressure or metheorological disturbance around, your thermometer should read 100?C when the water starts to boil. If there is a different reading (either higher or lower) that difference will be your thermometer correction, meaning that to whatever reading you have, you will have to add (+) or substract (-) that difference. For 0?C fill a container with distilled water and add ice cubes and, with the same provisions as before, the reading should be 0?C when there is a surface ice crust of ice in the water + ice. Once a year is enough OR you could buy a digital thermometer (for less that $35). ALL come certified and avoid all the hassle previously described! Ren? J. WWmn916@aol.com wrote: Hello everyone, I'm hoping for a little help. What is everyone using to calibrate routine thermometers in the histology lab (thermometers for refrig, H20 baths, IPOX machines....etc.)? We can no longer use mercury filled certified thermometers to test the others. Any suggestions? How often do the certified thermometers need to be recalibrated? Thanks much, Deb King California ************************************** See what's free at http://www.aol.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From rjbuesa <@t> yahoo.com Wed May 2 09:03:17 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 2 09:03:21 2007 Subject: [Histonet] MOHS In-Reply-To: <7E3ACD48BA6E26408F3188FBF08693F78B246F@titansbs1.corp.titanmed.com> Message-ID: <825625.80927.qm@web61214.mail.yahoo.com> In the last issue of Advance MLP comes an address that you could try: www.mohshistotemp.com Ren? J. April Sachau wrote: Good Morning, Can anyone please tell me why it has been impossible to find someone with strong MOHS experience? April Sachau Titan Medical Group Staff Supervisor Phone (866) 332-9600 Ext. 1023 Fax (402) 332-5181 asachau@titanmed.com see us on the web at www.titanmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of raghul Sent: Wednesday, May 02, 2007 8:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] alcian blue_precipitaion_solution dear histonetters hope atleast the persons remember my problem of alcian blue(sigma) precpitating in dis. water. I went on to stirr for 2 hours followed by boiling for 5 min. I could make a 2.5ph solution of alcian blue and its staining well. I think boiling was the main reason. thanks for all those suggestions raghul medclone biotech chennai, india --------------------------------- Check out what you're missing if you're not on Yahoo! Messenger _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From godsgalnow <@t> aol.com Wed May 2 09:05:14 2007 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Wed May 2 09:05:26 2007 Subject: [Histonet] looking for Freida Carson Message-ID: <8C95ADA0A9C8D49-D0C-449A@FWM-R13.sysops.aol.com> I am looking for Freida...can you please email me. Thanks, Roxanne Soto HT(ASCP)QIHC ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From Kemlo.Rogerson <@t> waht.swest.nhs.uk Wed May 2 09:59:29 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed May 2 09:59:39 2007 Subject: [Histonet] Certified thermometers Message-ID: <86ADE4EB583CE64799A9924684A0FBBF01247657@wahtntex2.waht.swest.nhs.uk> your thermometer should read 100?C when the water starts to boil. Rene If water boils longer does it get hotter? I thought water boiled at 100 degrees however long you left it; if you increased the heat it still boiled at 100 degrees but the increased latent heat of vaporisation meant that more water was able to vaporise but still only at 100 degrees. Kemlo For 0?C fill a container with distilled water and add ice cubes and, with the same provisions as before, the reading should be 0?C when there is a surface ice crust of ice in the water + ice. Rene Same with freezing; I thought water could be 'frozen' at the eutectic point of water which is +4 degrees C (that's how you get 'black ice'). It certainly is at its most dense (which is why ice floats) and wouldn't the bottom of the water be hotter than at the top cos the coldest water floats (its less dense at the eutectic point) that's why we are here are were able to crawl out of the sea and my Koi don't freeze in Winter. Never really understood water that why I used to brew beer in it. I'd buy a digital thermometer because those of you at sea level will soon drown anyway with global warming. Kemlo Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; The reason birds can fly and we can't is simply that they have perfect faith, for to have faith is to have wings. --J.M. Barrie This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From rjbuesa <@t> yahoo.com Wed May 2 10:07:44 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 2 10:07:50 2007 Subject: [Histonet] Certified thermometers In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBF01247657@wahtntex2.waht.swest.nhs.uk> Message-ID: <12704.11108.qm@web61221.mail.yahoo.com> That is why it is better to buy a certified digital thermometer, and budget for a new one every year! (Avoids all physical and methaphysical considerations!). Ren? J. Kemlo Rogerson wrote: your thermometer should read 100?C when the water starts to boil. Rene If water boils longer does it get hotter? I thought water boiled at 100 degrees however long you left it; if you increased the heat it still boiled at 100 degrees but the increased latent heat of vaporisation meant that more water was able to vaporise but still only at 100 degrees. Kemlo For 0?C fill a container with distilled water and add ice cubes and, with the same provisions as before, the reading should be 0?C when there is a surface ice crust of ice in the water + ice. Rene Same with freezing; I thought water could be 'frozen' at the eutectic point of water which is +4 degrees C (that's how you get 'black ice'). It certainly is at its most dense (which is why ice floats) and wouldn't the bottom of the water be hotter than at the top cos the coldest water floats (its less dense at the eutectic point) that's why we are here are were able to crawl out of the sea and my Koi don't freeze in Winter. Never really understood water that why I used to brew beer in it. I'd buy a digital thermometer because those of you at sea level will soon drown anyway with global warming. Kemlo Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; The reason birds can fly and we can't is simply that they have perfect faith, for to have faith is to have wings. --J.M. Barrie This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From soofias2 <@t> yahoo.com Wed May 2 10:26:06 2007 From: soofias2 <@t> yahoo.com (soofia siddiqui) Date: Wed May 2 10:46:09 2007 Subject: [Histonet] Certified thermometers In-Reply-To: <12704.11108.qm@web61221.mail.yahoo.com> Message-ID: <97858.21365.qm@web39515.mail.mud.yahoo.com> Instead of buying new digital thermometer every year, buying two sets and using them alternate year will be economical. Control company re-certifies our thermometers for $15 each. I send them two thermometers to recalibrate and it cost us $ 40( including shipping). Soofia _______________ Molecular diagnostics lab UWHC Madison WI --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From anh2006 <@t> med.cornell.edu Wed May 2 11:33:46 2007 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Wed May 2 11:33:55 2007 Subject: [Histonet] Aortic ring references Message-ID: Does anyone have PDF or faxable forms of the following articles? If so, send me a personal email. I don't have access but would love to read them: Nicosia, RF and Ottinetti A. Modulation of microvascular growth and morphogenesis by reconstituted basement membrane gel in three dimensional cultures of rat aorta: a comparative study of angiogenesis in matrigel, collagen, fibrin, and plasma clot. In Vitro Cell Dev Biol. 1990 Feb;26(2):119-28. Nicosia RF, Ottinetti A. Growth of microvessels in serum-free matrix culture of rat aorta. A quantitative assay of angiogenesis in vitro. Lab Invest. 1990 Jul;63(1):115-22. In addition, I am trying to fine tune my protocol for staining aortic ring explants. Is anyone willing to share their protocol with me? Thank you, Andrea -- From dmccaig <@t> ckha.on.ca Wed May 2 12:06:10 2007 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Wed May 2 12:06:19 2007 Subject: [Histonet] heated forcep warmer Message-ID: Can someone provide me with a supplier for a seperate heated focep unit for embedding stations? Thanks Diana McCaig From jqb7 <@t> cdc.gov Wed May 2 12:15:15 2007 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed May 2 12:15:22 2007 Subject: [Histonet] heated forcep warmer References: Message-ID: Timing is everything! I am sure there are many, but I was just looking through my new American Master Tech catalog that came in today's mail and they have one http://www.americanmastertech.com/store/main.aspx?p=ItemDetailStyles&ite m=EQFW-120 Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Wednesday, May 02, 2007 1:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] heated forcep warmer Can someone provide me with a supplier for a seperate heated focep unit for embedding stations? Thanks Diana McCaig _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DennisH <@t> cookchildrens.org Wed May 2 12:41:15 2007 From: DennisH <@t> cookchildrens.org (Dennis Hahn) Date: Wed May 2 12:41:34 2007 Subject: [Histonet] PA Net Info Message-ID: If one exists.....Does anyone know the site or email address for the PA version of the Histonet? Thanks in advance, Dennis Dennis Hahn, HT (ASCP) Histology Lab Supervisor Cook Children's Medical Center 801 7th Avenue Ft Worth, Tx 76104-2796 682-885-6168 dennish@cookchildrens.org ------------------------------------------------------------------------------------------------------------ Cook Children's Health Care System This e-mail, facsimile, or letter and any files or attachments transmitted may contain information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Cook Children's Health Care System immediately at (682)885-4000 or via e-mail at compliance@cookchildrens.org. Cook Children's Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. ----------------------------------------------------------------------------------------------------------- From djemge <@t> aol.com Wed May 2 12:43:42 2007 From: djemge <@t> aol.com (djemge@aol.com) Date: Wed May 2 12:44:03 2007 Subject: [Histonet] (no subject) Message-ID: <8C95AF88F6F7778-FE8-5AAF@mblk-r28.sysops.aol.com> Hello All. One of the Grad Students asked me to post her question. The Testes and Brain tissue for ISH Sox3 was kept at 4 degrees, 4% PFA fixed overnight, 30% sucrose cryoprotected until it sank, embedded in OCT, flash frozen. The ISH on the Brain was very good, but not the Testes. I tried to submit photos but they never appeared in the image gallery - maybe they will later. There are 2 image links for immunoportal.com below. Thx Donna Here's Monica's question: I would like to know if anyone else has experience with ISH on adult testis cross-sections and if they've seen this background staining that specifically binds to elongated spermatid heads... And of course if anyone has been able to block against this. My sox3 probe is supposed to bind to the undifferentiated spermatogonia along the basement membrane of the seminiferous tubules. This staining appeared before the positive staining (approximately 3-4h after incubation) among the spermatid heads towards the lumen of the tubules. I have been using 4%PFA fixed testes sections and blocking with 10% lamb serum. This staining has appeared with both an 820b probe and 440b probe. -- Monica M Laronda Graduate Student, Jameson Lab Northwestern University Feinberg School of Medicine Chicago, IL m-laronda@northwestern.edu Phone: 312-503-2036 http://immunoportal.com/albums/album02/2007_04_13_a2.thumb.jpg http://immunoportal.com/albums/album02/2007_04_13_10xa.thumb.jpg Donna J Emge, HT(ASCP) Northwestern University Feinberg School of Medicine Chicago, IL djemge@aol.com d-emge@northwestern.edu Phone: 312-503-2036 ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From djemge <@t> aol.com Wed May 2 12:49:39 2007 From: djemge <@t> aol.com (djemge@aol.com) Date: Wed May 2 12:49:45 2007 Subject: [Histonet] ISH Sox3 Mouse Testes Specific Background Staining Spermatids In-Reply-To: <8C95AF88F6F7778-FE8-5AAF@mblk-r28.sysops.aol.com> References: <8C95AF88F6F7778-FE8-5AAF@mblk-r28.sysops.aol.com> Message-ID: <8C95AF96443DEA6-FE8-5B41@mblk-r28.sysops.aol.com> -----Original Message----- From: djemge@aol.com To: histonet@lists.utsouthwestern.edu Sent: Wed, 2 May 2007 12:43 PM Hello All. One of the Grad Students asked me to post her question. The Testes and Brain tissue for ISH Sox3 was kept at 4 degrees, 4% PFA fixed overnight, 30% sucrose cryoprotected until it sank, embedded in OCT, flash frozen. The ISH on the Brain was very good, but not the Testes. I tried to submit photos but they never appeared in the image gallery - maybe they will later. There are 2 image links for immunoportal.com below. Thx Donna Here's Monica's question: I would like to know if anyone else has experience with ISH on adult testis cross-sections and if they've seen this background staining that specifically binds to elongated spermatid heads... And of course if anyone has been able to block against this. My sox3 probe is supposed to bind to the undifferentiated spermatogonia along the basement membrane of the seminiferous tubules. This staining appeared before the positive staining (approximately 3-4h after incubation) among the spermatid heads towards the lumen of the tubules. I have been using 4%PFA fixed testes sections and blocking with 10% lamb serum. This staining has appeared with both an 820b probe and 440b probe. -- Monica M Laronda Graduate Student, Jameson Lab Northwestern University Feinberg School of Medicine Chicago, IL m-laronda@northwestern.edu Phone: 312-503-2036 http://immunoportal.com/albums/album02/2007_04_13_a2.thumb.jpg http://immunoportal.com/albums/album02/2007_04_13_10xa.thumb.jpg Donna J Emge, HT(ASCP) Northwestern University Feinberg School of Medicine Chicago, IL djemge@aol.com d-emge@northwestern.edu Phone: 312-503-2036 AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From sbreeden <@t> nmda.nmsu.edu Wed May 2 13:23:33 2007 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed May 2 13:23:38 2007 Subject: [Histonet] Gill's 3? Message-ID: <4D14F0FC9316DD41972D5F03C070908B8F4437@nmdamailsvr.nmda.ad.nmsu.edu> Excuse the question if it is patently obvious - perhaps I'm having a Senior Moment. I occasionally do Congo Red (Sigma's kit) and it calls for Gill 3 Hematoxylin. According to the description of Gill 3, it is a regressive hematoxylin - as is Harris'. Should I not have Gill 3 at that moment, what would be the result (pos/neg) of using Harris'? A follow-on to this question: is there a vendor for Bouin's that sells less than a liter? Thanks in advance. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 From BMolinari <@t> heart.thi.tmc.edu Wed May 2 13:41:10 2007 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Wed May 2 13:41:14 2007 Subject: [Histonet] PSR Message-ID: After coverslipping, my picro Sirius red slides show a leaching of what seems to be the picric acid. I see a "halo" of yellow . I have tried extending the acidified water rinses and followed w/ 100% ETOH - Xylene . Any ideas? Thanks! Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC 1-283 Houston, TX 77030 832-355-6524 832-355-6812 (fax) From djemge <@t> aol.com Wed May 2 13:43:35 2007 From: djemge <@t> aol.com (djemge@aol.com) Date: Wed May 2 13:43:49 2007 Subject: [Histonet] In Situ Hybridization on Mouse Testes Fixed Frozen sections Message-ID: <8C95B00ED188D3E-4B8-62C8@WEBMAIL-MA11.sysops.aol.com> Hello All. One of the Grad Students asked me to post her question. The Testes and Brain tissue for ISH Sox3 was kept at 4 degrees, 4% PFA fixed overnight, 30% sucrose cryoprotected until it sank, embedded in OCT, flash frozen. The ISH on the Brain was very good, but not the Testes. Two photos ISH Sox 3 Testes and ISH Sox 3 Brain are in the image gallery at histonet.org. Thanks, Donna Here's Monica's question: I would like to know if anyone else has experience with ISH on adult testis cross-sections and if they've seen this background staining that specifically binds to elongated spermatid heads... And of course if anyone has been able to block against this. My sox3 probe is supposed to bind to the undifferentiated spermatogonia along the basement membrane of the seminiferous tubules. This staining appeared before the positive staining (approximately 3-4h after incubation) among the spermatid heads towards the lumen of the tubules. I have been using 4%PFA fixed testes sections and blocking with 10% lamb serum. This staining has appeared with both an 820b probe and 440b probe. -- Monica M Laronda Graduate Student, Jameson Lab Northwestern University Feinberg School of Medicine Chicago, IL m-laronda@northwestern.edu Phone: 312-503-2036 Donna J Emge, HT(ASCP) Northwestern University Feinberg School of Medicine Chicago, IL djemge@aol.com d-emge@northwestern.edu Phone: 312-503-2036 ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From sbreeden <@t> nmda.nmsu.edu Wed May 2 14:15:39 2007 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed May 2 14:15:44 2007 Subject: [Histonet] GILL 3 Confusion Message-ID: <4D14F0FC9316DD41972D5F03C070908B8F443C@nmdamailsvr.nmda.ad.nmsu.edu> I'm making this follow-up post because I'm getting some conflicting information about Gill 3 Hematoxylin. Quoting from the Sigma 2006-2007 Biochemicals, Reagents & Kits catalog, page 1196: "General purpose nuclear stain, regressive type. Used with hematoxylin and eosin staining." Thus my question about substituting Harris' for Gill 3. I've had a couple responses telling me that Gill 3 is PROGRESSIVE. So, whazzup??? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 From jjohnson <@t> crispregional.org Wed May 2 14:23:04 2007 From: jjohnson <@t> crispregional.org (Jennifer Johnson) Date: Wed May 2 14:26:01 2007 Subject: [Histonet] Touch Preps Message-ID: <000501c78cef$4ee6cf10$2082010a@main.crispregional.org> Are any of you doing Touch Preps on Breast tissue to determine whether or not the margins are free? If so, are you having success with this method and do you consider it to be better than a frozen section? From rjbuesa <@t> yahoo.com Wed May 2 14:37:19 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 2 14:37:22 2007 Subject: [Histonet] Gill's 3? In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B8F4437@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <368056.50533.qm@web61216.mail.yahoo.com> Sara: After Congo Red you will need a nuclear staining with hematoxylin, it does not matter which hematoxylin. Most definitely, you can use Harris (as a matter of fact, that was the one I always used). Ren? J. "Breeden, Sara" wrote: Excuse the question if it is patently obvious - perhaps I'm having a Senior Moment. I occasionally do Congo Red (Sigma's kit) and it calls for Gill 3 Hematoxylin. According to the description of Gill 3, it is a regressive hematoxylin - as is Harris'. Should I not have Gill 3 at that moment, what would be the result (pos/neg) of using Harris'? A follow-on to this question: is there a vendor for Bouin's that sells less than a liter? Thanks in advance. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From rjbuesa <@t> yahoo.com Wed May 2 14:38:24 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 2 14:38:27 2007 Subject: [Histonet] Touch Preps In-Reply-To: <000501c78cef$4ee6cf10$2082010a@main.crispregional.org> Message-ID: <515207.30512.qm@web61220.mail.yahoo.com> For margins it is always better a frozen section. Ren? J. Jennifer Johnson wrote: Are any of you doing Touch Preps on Breast tissue to determine whether or not the margins are free? If so, are you having success with this method and do you consider it to be better than a frozen section? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From gcallis <@t> montana.edu Wed May 2 14:48:26 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed May 2 14:48:35 2007 Subject: [Histonet] Gill's 3? In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B8F4437@nmdamailsvr.nmda.ad .nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B8F4437@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <6.0.0.22.1.20070502133844.01b3ce48@gemini.msu.montana.edu> No, is NOT regressive, Gill 3 is a progressive hematoxylin and it differs from Gill 1 by having a higher concentration of hematoxylin (3X more) hence the Gill 3. Staining is usually done in approx 1 1/2 minutes, then you rinse with approx 10 dips or so 4% acetic acid only to get rid of ionic interaction of hematoxylin to the glass slide and a bit of background staining in the tissue. The acetic acid is not a differentiation solution as is 1% HCl in 70% alcohol (as used with Harris Hematoxylin). I am not a fan of Harris anymore since it can no longer be made with mercuric oxide (boo hoo!) Harris tends to overstain, Gill will not do this. We switched to Gill or other progressive hematoxylins years ago to get away from having to differentiate the Harris. Simply, with Gill 3 you just stain, rinse with acetic, and blue in Scotts tap water substitute. Check out Richard Allan Gill hematoxylins, they sell everything you need and the stain was superb. Whoever wrote the Sigma tech sheet didn't know their hematoxylins IF they called Gill regressive!! Gary Gill must be gagging over this one. Gayle Callis (senior too!) At 12:23 PM 5/2/2007, you wrote: >Excuse the question if it is patently obvious - perhaps I'm having a >Senior Moment. I occasionally do Congo Red (Sigma's kit) and it calls >for Gill 3 Hematoxylin. According to the description of Gill 3, it is a >regressive hematoxylin - as is Harris'. Should I not have Gill 3 at >that moment, what would be the result (pos/neg) of using Harris'? A >follow-on to this question: is there a vendor for Bouin's that sells >less than a liter? Thanks in advance. > > > >Sally Breeden, HT(ASCP) > >NM Dept. of Agriculture > >Veterinary Diagnostic Services > >PO Box 700 > >Albuquerque, NM 87106 > >505-841-2576 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From Charles.Embrey <@t> carle.com Wed May 2 15:00:50 2007 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Wed May 2 15:00:58 2007 Subject: [Histonet] PA Net Info In-Reply-To: Message-ID: <44780C571F28624DBB446DE55C4D733A1FE4D3@EXCHANGEBE1.carle.com> There is, but it is restricted to registered PAs and available through the AAPA website, www.pathologistsassistants.org Charles Embrey PA(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dennis Hahn Sent: Wednesday, May 02, 2007 12:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PA Net Info If one exists.....Does anyone know the site or email address for the PA version of the Histonet? Thanks in advance, Dennis Dennis Hahn, HT (ASCP) Histology Lab Supervisor Cook Children's Medical Center 801 7th Avenue Ft Worth, Tx 76104-2796 682-885-6168 dennish@cookchildrens.org ------------------------------------------------------------------------ ------------------------------------ Cook Children's Health Care System This e-mail, facsimile, or letter and any files or attachments transmitted may contain information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Cook Children's Health Care System immediately at (682)885-4000 or via e-mail at compliance@cookchildrens.org. Cook Children's Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. ------------------------------------------------------------------------ ----------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kathy.Johnston <@t> CLS.ab.ca Wed May 2 15:03:46 2007 From: Kathy.Johnston <@t> CLS.ab.ca (Kathy.Johnston@CLS.ab.ca) Date: Wed May 2 15:04:45 2007 Subject: [Histonet] GILL 3 Confusion In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B8F443C@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <4BC300747AF87A48BCDF8E48BC2885CE049B9161@mail1.calgary.com> And yet on the two preceding pages, Gill 1 and Gill 2 are both described as progressive. Both Bancroft and Luna describe them as being regressive. Odd isn't it? I had always been taught that all Gill hematoxylin are progressive. I use my Sigma Gill 3 progressively, but I'm quite sure you could use it regressively if your procedure would allow it. I also use Harris' progressively and regressively some of my other procedures as well. I believe your choice of hematoxylins is all in how you want to use them and how you want your end product to look. For our Congo Red's we use Harris' regressively before the CR solutions. Kathy Johnston Tech II Special Stains - Anatomic Pathology Calgary Laboratory Services #9 - 3535 Research Road NW Calgary, AB Canada 403-770-3572 phone 403-770-3731 fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Breeden, Sara Sent: May 2, 2007 1:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GILL 3 Confusion I'm making this follow-up post because I'm getting some conflicting information about Gill 3 Hematoxylin. Quoting from the Sigma 2006-2007 Biochemicals, Reagents & Kits catalog, page 1196: "General purpose nuclear stain, regressive type. Used with hematoxylin and eosin staining." Thus my question about substituting Harris' for Gill 3. I've had a couple responses telling me that Gill 3 is PROGRESSIVE. So, whazzup??? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. From gcallis <@t> montana.edu Wed May 2 15:16:49 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed May 2 15:16:58 2007 Subject: [Histonet] GILL 3 Confusion In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B8F443C@nmdamailsvr.nmda.ad .nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B8F443C@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <6.0.0.22.1.20070502135115.01b11280@gemini.msu.montana.edu> I think Sigma, or whoever wrote the specifications are uninformed. The differences between Gill (progressive) and Harris (regressive) is discussed in most modern histotechnology textbooks including Sheehan and Hrapchak's book. Gill 1,2, or 3 are progressive and there have been some delightful spinoffs on this formulation for excellent H&E staining. Publication is Gill G et al. A new formula for a half oxidized hematoxylin solution that neither over-stains nor requires differentiation. Acta Cytologica, 18:300-311, 1974. The title itself indicates this is NOT a regressive hematoxylin. When commercial Gill first came out, we bought ours from Lerner Laboratories before everyone started to make Gill 1, 2, and 3, and I still have the spec sheet on file. Staining is done in 1 - 5 minutes, rinse, 4% acetic acid rinse 10 dips, tap water rinse, Bluing reagent (Scotts Tap water, or Richard Allan Bluing solution, pH 8) 10 dips (longer is permitted) then water rinse. Some people will decolorize Gill hematoxylins. However, Gills publication, and another by Meloan and Puchtler titled Harris Hematoxylin, What Harris really wrote and the mechanism of hemalum stains. J Histotechnology 10(4):257, 1987 - you learn Gills do not need decolorizing per se with an acid/alcohol. I think one reason people tend to decolorize Gill is they stain too long in the solutions or use the more concentrated Gill 3 when Gill 2 will do the job. We always tried to control for optimal staining by time in the Gill rather than removing it later. Our staining time was usually 1 1/2 to 2 minutes in Gill 2. I am forever thankful to Gary Gill for making my hematoxylin and eosin staining "life" easier!! He also has made comments on Histonet, so check out the archives. Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 PuchAt 01:15 PM 5/2/2007, you wrote: >I'm making this follow-up post because I'm getting some conflicting >information about Gill 3 Hematoxylin. Quoting from the Sigma 2006-2007 >Biochemicals, Reagents & Kits catalog, page 1196: "General purpose >nuclear stain, regressive type. Used with hematoxylin and eosin >staining." Thus my question about substituting Harris' for Gill 3. >I've had a couple responses telling me that Gill 3 is PROGRESSIVE. So, >whazzup??? > > > >Sally Breeden, HT(ASCP) > >NM Dept. of Agriculture > >Veterinary Diagnostic Services > >PO Box 700 > >Albuquerque, NM 87106 > >505-841-2576 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bethcoxx <@t> gmail.com Wed May 2 15:19:58 2007 From: bethcoxx <@t> gmail.com (Beth Cox) Date: Wed May 2 15:20:03 2007 Subject: [Histonet] RE: Cell Button Prep Message-ID: Hi Karen, It is generally recommended that you spin down your fluid, decant, and then add the fixative and re-spin. Using a 1/2 fluid and 1/2 fixative method gives you a weak solution for fixation. Whether you use 95% alcohol (ethyl or reagent) or 10% formalin is a personal choice - it depends on whether your pathologist likes to see formalin fixed morphology or alcohol fixed morphology. You didn't mention what method you use to hold all those loose cells together for processing/embedding/sectioning. There are several simple methods available to make your life easier and give the most cellular blocks/slides. If you are interested in info about those, just let me know. Beth Cox, SCT/HT(ASCP) Schools of Histotechnology Department of Anatomic Pathology, 100RO William Beaumont Hospital 3601 W. 13 Mile Road Royal Oak, MI 48073-6769 Work 248/898-9020 Fax 248/898-9054 E-mail bethcox@beaumonthospitals.com Date: Tue, 1 May 2007 12:51:36 -0700 From: "Heckford, Karen - SMMC-SF" Subject: [Histonet] Cell Button Prep To: "Histonet (histonet@lists.utsouthwestern.edu)" Message-ID: Content-Type: text/plain; charset="us-ascii" I was wondering if anyone would be so kind as giving input on the different ways of preparing a cell button. We work a lot with Pleural Fluid and Ascites Fluid. I have been told two different ways. One is just 1/2 and 1/2 with fluid and 95%alcohol and spend down decant and get your cell button. The other is spend down the fluid decant add 10%formalin respin and let set then get your cell button. Of course this all after I have made my cytospins. We do not have a ThinPrep type machine. Any input would be very appreciative. I realize there is probably more ways to do this that I have not heard of. Cheers, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathology Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu From jwpmail <@t> gmail.com Wed May 2 15:51:24 2007 From: jwpmail <@t> gmail.com (Joe Plandowski) Date: Wed May 2 15:51:32 2007 Subject: [Histonet] Seeking Histotech - Paramus (NJ) Message-ID: A new anatomic pathology laboratory is seeking a histotech. Great opportunity, great community and excellent compensation. Please send resume. From ftulenko06 <@t> jcu.edu Wed May 2 15:52:54 2007 From: ftulenko06 <@t> jcu.edu (ftulenko06@jcu.edu) Date: Wed May 2 15:52:59 2007 Subject: [Histonet] anti human placental alkaline phosphatase Message-ID: <20070502165254.AFB42890@mirapoint.jcu.edu> Hello Histonetters, I was hoping to receive advice on finding an anti-human placental alkaline phosphatase antibody. The lab I work in is using transgenic mice that express human placental alkaline phosphatase as a tissue specific reporter. Any advice would be appreciated. It seems that most antibodies I have found are produced in mice. Has anybody used these and is there a problem with mouse-on-mouse background. Thank you, Frank Tulenko From Charles.Embrey <@t> carle.com Wed May 2 16:11:03 2007 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Wed May 2 16:11:08 2007 Subject: [Histonet] 2 open HT positions Message-ID: <44780C571F28624DBB446DE55C4D733A1FE4D5@EXCHANGEBE1.carle.com> I have 2 open HT positions at Carle Clinic in Urbana Illinois, home of the Fighting Illini. One position is for a dayshift HT and the other is for dayshift Technical Specialist. Carle has a great working environment and is the largest Hospital in the region. The histology lab processes an average of 225 blocks a day. We are currently moving to microwave processing. Please contact me for more info. Charles Embrey PA(ASCP) Histology Manager Carle Clinic, Urbana Illinois From wjtang <@t> stanford.edu Wed May 2 18:06:39 2007 From: wjtang <@t> stanford.edu (wjtang@stanford.edu) Date: Wed May 2 18:05:22 2007 Subject: [Histonet] storing paraformaldehyde under inert gas Message-ID: <20070502160639.megui4a1gq4ok80g@webmail.stanford.edu> Hi, We just ordered paraformaldehyde (crystalline form, Sigma P6148) and found out from the MSDS that it needs to be stored under inert gas at 4C. Does anyone know how to do store a reagent under inert gas? Or does anyone do this at all w/ their paraformaldehyde? I tried searching the Histonet archives and didn't find any info relating to storing the solid but maybe I used the wrong search terms. Thanks in advance for any assistance! -- Joyce Tang Cell and Molecular Biomechanics Laboratory Stanford University From JMacDonald <@t> mtsac.edu Wed May 2 23:07:04 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed May 2 23:07:08 2007 Subject: [Histonet] PSR In-Reply-To: Message-ID: What type of mounting medium are you using? Jennifer Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu "Molinari, Betsy" Sent by: histonet-bounces@lists.utsouthwestern.edu 05/02/2007 11:41 AM To cc Subject [Histonet] PSR After coverslipping, my picro Sirius red slides show a leaching of what seems to be the picric acid. I see a "halo" of yellow . I have tried extending the acidified water rinses and followed w/ 100% ETOH - Xylene . Any ideas? Thanks! Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC 1-283 Houston, TX 77030 832-355-6524 832-355-6812 (fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Thu May 3 01:56:59 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu May 3 01:57:08 2007 Subject: [Histonet] Gill's 3? Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0124765C@wahtntex2.waht.swest.nhs.uk> I think any Harris's or Gill's both act as a regressive stain if you overstain or if they are overoxidised. The trick is to get your staining times correct with Gill's and use in date stain. I used Gill 3 for cervical smears and it worked very well but I tended always to differentiate either out of habit of because I wanted crisper counterstain; Harris's always appeared muddy. Never understand Gill 1 or 2 though, but then I never got my head around EA50 and EA65 so that's not unusual. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; The reason birds can fly and we can't is simply that they have perfect faith, for to have faith is to have wings. --J.M. Barrie This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Kemlo.Rogerson <@t> waht.swest.nhs.uk Thu May 3 02:04:33 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu May 3 02:04:38 2007 Subject: [Histonet] Touch Preps Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0124765D@wahtntex2.waht.swest.nhs.uk> Are any of you doing Touch Preps on Breast tissue to determine whether or not the margins are free? If so, are you having success with this method and do you consider it to be better than a frozen section? Jennifer Depends what you are trying to do. If you have a tissue diagnosis and all you want to do is to check for malignancy or not then I guess touch preps are fine. If you need a tissue diagnosis then I guess not. We use FNAC for the diagnosis of breast cancer in our one stop breast Clinics and I have done them for many years; breast cytology, like so much in cytology, is only as good as the Cytologist (in some cases) and that Cytologist must know the limitations of the technique. IMHO once you know the pitfalls and when to say "I don't know" then Cytology is useful but I feel it is more 'subjective' than Histology where you get more information. I am not a Clinician nor medically qualified but have been 'doing' cytology for 25 years and these are only my personal views. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; The reason birds can fly and we can't is simply that they have perfect faith, for to have faith is to have wings. --J.M. Barrie This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Kemlo.Rogerson <@t> waht.swest.nhs.uk Thu May 3 02:06:05 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu May 3 02:06:09 2007 Subject: [Histonet] GILL 3 Confusion Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0124765E@wahtntex2.waht.swest.nhs.uk> As I've already said, depends on the age of the stain, depends on the length of time for staining. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; The reason birds can fly and we can't is simply that they have perfect faith, for to have faith is to have wings. --J.M. Barrie This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From BMolinari <@t> heart.thi.tmc.edu Thu May 3 05:14:37 2007 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Thu May 3 05:14:43 2007 Subject: [Histonet] PSR In-Reply-To: <8B07D141BCDE434285DC12B3290E3FB3010B0E55@exbackca.caus.dako.net> Message-ID: RA Mounting Media on my glass coverslipper. I will contact my RA rep to ask if this is the case. Thanks! Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: Sarah Jones [mailto:Sarah.Jones@dako.com] Sent: Wednesday, May 02, 2007 1:57 PM To: Molinari, Betsy Subject: RE: [Histonet] PSR What kind of mounting media are you using, Betsy? Sarah Sarah A. Jones, HTL (ASCP) CM Research Associate, R&D Artisan Special Stains Dako North America, Inc. 6392 Via Real Carpinteria, CA 93013 sarah.jones@dako.com Tel (800) 235-5743 Ext 5681 Fax (805) 684-1372 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Wednesday, May 02, 2007 11:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PSR After coverslipping, my picro Sirius red slides show a leaching of what seems to be the picric acid. I see a "halo" of yellow . I have tried extending the acidified water rinses and followed w/ 100% ETOH - Xylene . Any ideas? Thanks! Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC 1-283 Houston, TX 77030 832-355-6524 832-355-6812 (fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BMolinari <@t> heart.thi.tmc.edu Thu May 3 05:17:09 2007 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Thu May 3 05:17:11 2007 Subject: [Histonet] PSR In-Reply-To: <6.0.0.22.1.20070502134955.01b461a8@gemini.msu.montana.edu> Message-ID: Thanks Gail I will give it a go. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: Wednesday, May 02, 2007 2:51 PM To: Molinari, Betsy Subject: Re: [Histonet] PSR Betsty, After staining, try blotting the slide to dry, then coverslip without going through the alcohols, etc - just like van Giesons. Gayle Callis At 12:41 PM 5/2/2007, you wrote: >After coverslipping, my picro Sirius red slides show a leaching of what >seems to be the picric acid. I see a "halo" of yellow . I have tried >extending the acidified water rinses and followed w/ 100% ETOH - Xylene >. Any ideas? >Thanks! > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From JWEEMS <@t> sjha.org Thu May 3 05:45:31 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Thu May 3 05:45:57 2007 Subject: [Histonet] PSR In-Reply-To: Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FF96FA@sjhaexc02.sjha.org> We use Cytoceal XY -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Thursday, May 03, 2007 12:07 AM To: Molinari, Betsy Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] PSR What type of mounting medium are you using? Jennifer Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu "Molinari, Betsy" Sent by: histonet-bounces@lists.utsouthwestern.edu 05/02/2007 11:41 AM To cc Subject [Histonet] PSR After coverslipping, my picro Sirius red slides show a leaching of what seems to be the picric acid. I see a "halo" of yellow . I have tried extending the acidified water rinses and followed w/ 100% ETOH - Xylene . Any ideas? Thanks! Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC 1-283 Houston, TX 77030 832-355-6524 832-355-6812 (fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From sbreeden <@t> nmda.nmsu.edu Thu May 3 07:04:07 2007 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu May 3 07:04:12 2007 Subject: [Histonet] Gill 3 Thanks Message-ID: <4D14F0FC9316DD41972D5F03C070908B8F444A@nmdamailsvr.nmda.ad.nmsu.edu> My posting about the use of Harris' hematoxylin in lieu of Gill 3 (in Congo Red stain) brought many interesting responses and I thank each of you for your input. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 From jmahoney <@t> alegent.org Thu May 3 07:21:30 2007 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Thu May 3 07:21:48 2007 Subject: [Histonet] Open Position Message-ID: <46398D7A0200003C0000CAA9@gwia.alegent.org> Good Morning Histonetters, I have an immediate opening for a full or part time Histology Tech. If you are interested in gaining the experience of working in a LEAN laboratory with a highly motivated TEAM apply online at www.alegent.com We are located in Omaha Nebraska (home of the College World Series for you baseball fans). We offer a full benefit package and are an equal opportunity employer. Alegent Health is a health system with 5 metro hospitals, three out state hospitals and about 100 clinics. We are the largest employer in Omaha and attract the best employees throughout the region. Please call me anytime if you have questions about our lab or to see if you could be part of our Histology Team. Jan Janice Mahoney Histology/Cytology Coordinator Alegent Health Laboratory 4955 F Street Omaha, NE 68117 (402)717-2889 From jo-ann.bader <@t> mcgill.ca Thu May 3 08:39:32 2007 From: jo-ann.bader <@t> mcgill.ca (Jo-Ann Bader, Ms.) Date: Thu May 3 08:42:19 2007 Subject: [Histonet] DAKO autostainer Message-ID: I have an offer to purchase a DAKO autostainer second hand. I know it has not been used very much. The offer is for $40,000.00, half the purchase price. I would appreciate hearing from those of you who have used one an what you think of it. Thanks Jo-Ann Bader Histology Facility Coordinator Molecular Oncology Group MUHC/RVH 687 Pine Ave. W - Rm. M11-53 Montreal, QC, H3A-1A1 Tel: 514-934-1934 Ext: 31780 Fax: 514-843-1479 email: jo-ann.bader@mcgill.ca From HornHV <@t> archildrens.org Thu May 3 08:50:39 2007 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu May 3 08:50:51 2007 Subject: [Histonet] DAKO autostainer In-Reply-To: Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB7119@EMAIL.archildrens.org> The autostainer is a very good IHC stainer. I love mine and I think you would be pleased with it. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jo-Ann Bader, Ms. Sent: Thursday, May 03, 2007 8:40 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] DAKO autostainer I have an offer to purchase a DAKO autostainer second hand. I know it has not been used very much. The offer is for $40,000.00, half the purchase price. I would appreciate hearing from those of you who have used one an what you think of it. Thanks Jo-Ann Bader Histology Facility Coordinator Molecular Oncology Group MUHC/RVH 687 Pine Ave. W - Rm. M11-53 Montreal, QC, H3A-1A1 Tel: 514-934-1934 Ext: 31780 Fax: 514-843-1479 email: jo-ann.bader@mcgill.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From vazquezr <@t> ohsu.edu Thu May 3 08:52:43 2007 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Thu May 3 08:53:13 2007 Subject: [Histonet] Gill's 3? Message-ID: Gayle, Is this the same with frozen sections too (4% acetic acid)? I use a linear stainer, so a 4% solution would be too strong for the length of time it would be on the chain link. I would be using a 30sec cup. Could I weaken the solution to accomadate the time? Thanks Robyn >>> "Gayle Callis" 5/2/2007 12:48 PM >>> No, is NOT regressive, Gill 3 is a progressive hematoxylin and it differs from Gill 1 by having a higher concentration of hematoxylin (3X more) hence the Gill 3. Staining is usually done in approx 1 1/2 minutes, then you rinse with approx 10 dips or so 4% acetic acid only to get rid of ionic interaction of hematoxylin to the glass slide and a bit of background staining in the tissue. The acetic acid is not a differentiation solution as is 1% HCl in 70% alcohol (as used with Harris Hematoxylin). I am not a fan of Harris anymore since it can no longer be made with mercuric oxide (boo hoo!) Harris tends to overstain, Gill will not do this. We switched to Gill or other progressive hematoxylins years ago to get away from having to differentiate the Harris. Simply, with Gill 3 you just stain, rinse with acetic, and blue in Scotts tap water substitute. Check out Richard Allan Gill hematoxylins, they sell everything you need and the stain was superb. Whoever wrote the Sigma tech sheet didn't know their hematoxylins IF they called Gill regressive!! Gary Gill must be gagging over this one. Gayle Callis (senior too!) At 12:23 PM 5/2/2007, you wrote: >Excuse the question if it is patently obvious - perhaps I'm having a >Senior Moment. I occasionally do Congo Red (Sigma's kit) and it calls >for Gill 3 Hematoxylin. According to the description of Gill 3, it is a >regressive hematoxylin - as is Harris'. Should I not have Gill 3 at >that moment, what would be the result (pos/neg) of using Harris'? A >follow-on to this question: is there a vendor for Bouin's that sells >less than a liter? Thanks in advance. > > > >Sally Breeden, HT(ASCP) > >NM Dept. of Agriculture > >Veterinary Diagnostic Services > >PO Box 700 > >Albuquerque, NM 87106 > >505-841-2576 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu May 3 09:05:11 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu May 3 09:05:16 2007 Subject: [Histonet] DAKO autostainer In-Reply-To: Message-ID: <706068.73805.qm@web61224.mail.yahoo.com> IF it is in good condition and IF you can get a repair (parts and labor) reasonably priced contract, do not hesitate and buy it (once you have covered any possible but infrequent mechanical problems repaires). For me the DAKO autostainer is a very good piece of equipment. Ren? J. "Jo-Ann Bader, Ms." wrote: I have an offer to purchase a DAKO autostainer second hand. I know it has not been used very much. The offer is for $40,000.00, half the purchase price. I would appreciate hearing from those of you who have used one an what you think of it. Thanks Jo-Ann Bader Histology Facility Coordinator Molecular Oncology Group MUHC/RVH 687 Pine Ave. W - Rm. M11-53 Montreal, QC, H3A-1A1 Tel: 514-934-1934 Ext: 31780 Fax: 514-843-1479 email: jo-ann.bader@mcgill.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From mjlco <@t> aol.com Thu May 3 09:15:29 2007 From: mjlco <@t> aol.com (mjlco@aol.com) Date: Thu May 3 09:15:37 2007 Subject: [Histonet] DAKO autostainer In-Reply-To: References: Message-ID: <8C95BA4A38941F0-A68-C34F@mblk-d46.sysops.aol.com> Jo-Ann, we find it to be a great work horse. Well worth it. Matt Longmont United Hospital Colorado -----Original Message----- From: jo-ann.bader@mcgill.ca To: Histonet@lists.utsouthwestern.edu Sent: Thu, 3 May 2007 7:39 AM Subject: [Histonet] DAKO autostainer I have an offer to purchase a DAKO autostainer second hand. I know it has not been used very much. The offer is for $40,000.00, half the purchase price. I would appreciate hearing from those of you who have used one an what you think of it. Thanks Jo-Ann Bader Histology Facility Coordinator Molecular Oncology Group MUHC/RVH 687 Pine Ave. W - Rm. M11-53 Montreal, QC, H3A-1A1 Tel: 514-934-1934 Ext: 31780 Fax: 514-843-1479 email: jo-ann.bader@mcgill.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From shive003 <@t> umn.edu Thu May 3 10:34:31 2007 From: shive003 <@t> umn.edu (Jan Shivers) Date: Thu May 3 10:34:47 2007 Subject: [Histonet] DAKO autostainer References: Message-ID: <001d01c78d98$8ba4d790$a1065486@auxs.umn.edu> Jo-Ann, I have 2 DAKO autostainers and I would highly recommend them. Very easy to program and utilize; versatile open system. Jan Shivers Univ. of Minnesota Veterinary Diagnostic Lab St. Paul, MN 55108 shive003@umn.edu ----- Original Message ----- From: "Jo-Ann Bader, Ms." To: Sent: Thursday, May 03, 2007 8:39 AM Subject: [Histonet] DAKO autostainer I have an offer to purchase a DAKO autostainer second hand. I know it has not been used very much. The offer is for $40,000.00, half the purchase price. I would appreciate hearing from those of you who have used one an what you think of it. Thanks Jo-Ann Bader Histology Facility Coordinator Molecular Oncology Group MUHC/RVH 687 Pine Ave. W - Rm. M11-53 Montreal, QC, H3A-1A1 Tel: 514-934-1934 Ext: 31780 Fax: 514-843-1479 email: jo-ann.bader@mcgill.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Thu May 3 10:42:50 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Thu May 3 10:43:16 2007 Subject: [Histonet] DAKO autostainer In-Reply-To: <001d01c78d98$8ba4d790$a1065486@auxs.umn.edu> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FF9714@sjhaexc02.sjha.org> Same here! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jan Shivers Sent: Thursday, May 03, 2007 11:35 AM To: Jo-Ann Bader, Ms.; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] DAKO autostainer Jo-Ann, I have 2 DAKO autostainers and I would highly recommend them. Very easy to program and utilize; versatile open system. Jan Shivers Univ. of Minnesota Veterinary Diagnostic Lab St. Paul, MN 55108 shive003@umn.edu ----- Original Message ----- From: "Jo-Ann Bader, Ms." To: Sent: Thursday, May 03, 2007 8:39 AM Subject: [Histonet] DAKO autostainer I have an offer to purchase a DAKO autostainer second hand. I know it has not been used very much. The offer is for $40,000.00, half the purchase price. I would appreciate hearing from those of you who have used one an what you think of it. Thanks Jo-Ann Bader Histology Facility Coordinator Molecular Oncology Group MUHC/RVH 687 Pine Ave. W - Rm. M11-53 Montreal, QC, H3A-1A1 Tel: 514-934-1934 Ext: 31780 Fax: 514-843-1479 email: jo-ann.bader@mcgill.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From scorpionrider <@t> cox.net Thu May 3 10:55:05 2007 From: scorpionrider <@t> cox.net (scorpionrider@cox.net) Date: Thu May 3 10:55:08 2007 Subject: [Histonet] DAKO autostainer Message-ID: <11483605.1178207705544.JavaMail.root@fed1wml23.mgt.cox.net> We have 4 stainers and they are great! The only thing that comes to mind when purchasing used equipment is whether your institution would rather spend the asdditional dollars and get new instruments with the accompanying warranties and service contracts, or are they willing to take a short-cut to save the bucks. Somtimes you can even work arrangements of an instrument in return for standing orders of reagents, depending upon your usage. Either way should work as these are good machines. Mark Turner, HT(ASCP) Mayo Clinic Arizona ---- "Weems wrote: > Same here! > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 404-851-7376 - Phone > 404-851-7831 - Fax > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jan > Shivers > Sent: Thursday, May 03, 2007 11:35 AM > To: Jo-Ann Bader, Ms.; Histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] DAKO autostainer > > > Jo-Ann, > > I have 2 DAKO autostainers and I would highly recommend them. Very easy to > program and utilize; versatile open system. > > Jan Shivers > Univ. of Minnesota Veterinary Diagnostic Lab > St. Paul, MN 55108 > shive003@umn.edu > > > ----- Original Message ----- > From: "Jo-Ann Bader, Ms." > To: > Sent: Thursday, May 03, 2007 8:39 AM > Subject: [Histonet] DAKO autostainer > > > > I have an offer to purchase a DAKO autostainer second hand. I know it has > not been used very much. The offer is for $40,000.00, half the purchase > price. I would appreciate hearing from those of you who have used one an > what you think of it. > > Thanks > Jo-Ann Bader > Histology Facility Coordinator > Molecular Oncology Group > MUHC/RVH > 687 Pine Ave. W - Rm. M11-53 > Montreal, QC, H3A-1A1 > Tel: 514-934-1934 Ext: 31780 > Fax: 514-843-1479 > email: jo-ann.bader@mcgill.ca > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From HParker <@t> Skaggs.Net Thu May 3 11:11:18 2007 From: HParker <@t> Skaggs.Net (Parker, Helayne) Date: Thu May 3 11:12:17 2007 Subject: [Histonet] Silver Cassette Organizers In-Reply-To: <20070503104838.453421538D@barracuda.skaggs.net> Message-ID: <2FAF8CC41C5AAF43AAA52F26782E1A56FE44B4@mail1-schc.skaggs.net> Does anyone know where I can purchase those square brushed silver cassette holders that hold 15 empty cassettes (3 across in 5 rows). We use them when we set up the grosses on large specimens. We only have 3 and our Pathologist likes these organizers a lot. I can not find them to buy anywhere. Please advise. Thanks, Helayne Parker HT (ASCP) Histology Section Head Skaggs Community Health Center Branson, Missouri From kwalker <@t> valleyhealthlink.com Thu May 3 11:22:35 2007 From: kwalker <@t> valleyhealthlink.com (Walker, Keith) Date: Thu May 3 11:21:40 2007 Subject: [Histonet] specimens recieved in formalin Message-ID: We recently had our JCAHO survey done and one thing they were concerned about was formalin being applied to specimens in places other than our lab. I understand that proper ventilation and minimal exposure is a concern. We operate our lab Monday thru Friday from 4 am to 6 pm and on Saturdays from 4am to noon, so sending specimens over fresh would be a concern. We were wondering what other facilities do with similar situations. We are a 411 bed hospital with a large ER with level II trauma, a 15 room OR, an outpatient surgery center, full obstetrics, very busy endoscopy and colonoscopy business, and of course routine specimens from the floors. We are also a reference lab. We do about 22,000 surgical cases a year. We would also like to know if anyone else has had this issue brought up to them by inspectors before and how did they handle it. Thanks for your help Keith Walker HT ascp From cormier <@t> MIT.EDU Thu May 3 11:30:42 2007 From: cormier <@t> MIT.EDU (Kathy Cormier) Date: Thu May 3 11:30:51 2007 Subject: [Histonet] ? Qdotsq Message-ID: <001201c78da0$64d394a0$92003712@mit.edu> Hey Everyone, Another question for the collective knowledge bank out there. We are working w/ Qdot 525 goat anti rabbit fab fragment on FFPE mouse tissue. We are trying to get this to work with one of our usual antibodies ( that we use FITC and CY3 on) that routinely works on FFPE mouse tissue. We are getting no signal on the Qdot slides (our regular slides are fine). Has anyone gotten Qdots to work routinely on FFPE tissue? Will this Qdot be visible w/ out buying the very specific filter sets for Qdots? The pathologist insists that they should be visible w/ regular filter sets, not the very expensive sets. I am using the Immunoedge pens, and the Cytoseal 60 that is recommended by the spec sheet. Any suggestions or ideas? Thanks! Kathy Cormier Div Comp Medicine MIT From HornHV <@t> archildrens.org Thu May 3 11:40:42 2007 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu May 3 11:41:06 2007 Subject: [Histonet] specimens recieved in formalin In-Reply-To: Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB711C@EMAIL.archildrens.org> As far as I know, this has not been a problem here at my hospital. We receive 95% of our specimens in formalin. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Walker, Keith Sent: Thursday, May 03, 2007 11:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] specimens recieved in formalin We recently had our JCAHO survey done and one thing they were concerned about was formalin being applied to specimens in places other than our lab. I understand that proper ventilation and minimal exposure is a concern. We operate our lab Monday thru Friday from 4 am to 6 pm and on Saturdays from 4am to noon, so sending specimens over fresh would be a concern. We were wondering what other facilities do with similar situations. We are a 411 bed hospital with a large ER with level II trauma, a 15 room OR, an outpatient surgery center, full obstetrics, very busy endoscopy and colonoscopy business, and of course routine specimens from the floors. We are also a reference lab. We do about 22,000 surgical cases a year. We would also like to know if anyone else has had this issue brought up to them by inspectors before and how did they handle it. Thanks for your help Keith Walker HT ascp _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From rjbuesa <@t> yahoo.com Thu May 3 11:52:23 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu May 3 11:52:28 2007 Subject: [Histonet] specimens recieved in formalin In-Reply-To: Message-ID: <884860.81060.qm@web61214.mail.yahoo.com> With "formalin being applied to specimens in places other than our lab", do you mean that vials with formalin are available in other places than your lab to place specimens into them? If that is your question, in our hospital we used to have vials with formalin available in several places through the hospital for collected specimens. There was not a problem with that, provided that the vials with the formalin were in just one place in those receiving areas and that they were properly labelled. The problem could be in opening the vials to place the specimens in an area with poor ventilation. That could be the source of concern. Hope this will help you. Ren? J. "Walker, Keith" wrote: We recently had our JCAHO survey done and one thing they were concerned about was formalin being applied to specimens in places other than our lab. I understand that proper ventilation and minimal exposure is a concern. We operate our lab Monday thru Friday from 4 am to 6 pm and on Saturdays from 4am to noon, so sending specimens over fresh would be a concern. We were wondering what other facilities do with similar situations. We are a 411 bed hospital with a large ER with level II trauma, a 15 room OR, an outpatient surgery center, full obstetrics, very busy endoscopy and colonoscopy business, and of course routine specimens from the floors. We are also a reference lab. We do about 22,000 surgical cases a year. We would also like to know if anyone else has had this issue brought up to them by inspectors before and how did they handle it. Thanks for your help Keith Walker HT ascp _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From RJLevier <@t> LancasterGeneral.org Thu May 3 12:09:37 2007 From: RJLevier <@t> LancasterGeneral.org (LeVier, Rebecca J) Date: Thu May 3 12:09:45 2007 Subject: [Histonet] Ventana Symphony Message-ID: <0FDBF29200C637468CCC1F9E7E85A3D2019E39E5@MAIL-LR.lha.org> Hi Everyone, If anyone is using the Ventana Symphony, could you please let me know what your experience has been with it and how much time you are saving, etc. Thanks in advance. Becky Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From fawn <@t> cs.cmu.edu Thu May 3 12:21:03 2007 From: fawn <@t> cs.cmu.edu (Fawn Jones) Date: Thu May 3 12:20:45 2007 Subject: [Histonet] Job opening Message-ID: <463A19FF.2010208@cs.cmu.edu> Hi everyone, There is a possible histology job opening up at Carnegie Mellon Univeristy in Pittsburgh. This person will be dealing mainly with MMA plastic embedding and sectioning. It is a great opportunity for anyone interested. If you are interested or have any questions please contact me through e-mail at Fawn@cs.cmu.edu or call 412-268-8275 Thanks Fawn Jones From Karen.Heckford <@t> CHW.edu Thu May 3 12:30:08 2007 From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF) Date: Thu May 3 12:30:58 2007 Subject: [Histonet] Thermo Rep Message-ID: Does anyone know who the Thermo Rep. is for San Francisco Bay area. I need to talk to them. Emailing the company has failed. I guess it must be the switch over to Fisher? AUGH!!!! Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathology Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu From jerry.santiago <@t> jax.ufl.edu Thu May 3 12:33:38 2007 From: jerry.santiago <@t> jax.ufl.edu (Santiago, Jerry) Date: Thu May 3 12:34:21 2007 Subject: [Histonet] Telomerase Message-ID: <816DC61E1730E843A0BCF4CCFA1EFCF308928D@jaxmail.umc.ufl.edu> Histonetters: Is anyone using Telomerase on the Benchmark XT? If so, which antibody and clone? and can you share your protocol? Sincerely, Jerry Santiago, BS, HTL(ASCP)QIHC Shands Jacksonville Tumor Analysis Lab Jacksonville, Florida Lab: 904-244-6149 From TJJ <@t> Stowers-Institute.org Thu May 3 13:58:18 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Thu May 3 13:58:37 2007 Subject: [Histonet] RE: Silver Cassette Organizers In-Reply-To: Message-ID: Helayne, you mean like this? http://www.americanmastertech.com/store/main.aspx?p=ItemDetailStyles&ite m=FMML6146 They're not exactly the same as the old tissue tek organizers. These work with the unicassettes that have plastic lids attached. Hope this helps! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From kasamayoa <@t> gmail.com Thu May 3 14:21:12 2007 From: kasamayoa <@t> gmail.com (Kimberly Samayoa) Date: Thu May 3 14:21:17 2007 Subject: [Histonet] IHC on LR White hair follicles Message-ID: <335a28840705031221y21cc8de3p2191e8ad8cf29af8@mail.gmail.com> Hi Histonetters, I am new at working with resins and have been tasked to embed eyebrow hair in LR White for IHC staining of the hair follicles. I thermally cured the resin at 60C for 24 hours and sectioned the blocks at 2 micron. I dried the slides for 1 hour at 60C and open to air overnight. I am stumped because I am not sure how or where to start next. The antibodies I'm going to use I have used in paraffin sucessfully, however, they require pretreatment (Citrate buffer, pH 5.5 in a pressure cooker). I also read somewhere that H2O2 can make the sections come off the slide, so is endogenous H2O2 blocking needed? How does the DAB work with LR White? Does the resin need to be removed prior to staining? Lastly, I will be using JB4 in the near future and am curious to hear from people who use it if it is compatible for IHC. Is there a book someone could suggest that would be a good guide for how to work with resins? I appreciate any advice and thank you in advance. -- Kimberly A. Samayoa Work Direct: (650) 624-3295 From funderwood <@t> mcohio.org Thu May 3 14:23:14 2007 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Thu May 3 14:23:39 2007 Subject: [Histonet] Silver Cassette Organizers Message-ID: Hi Helayne, This company offers a plastic version that may work. Fred http://www.innovativelabacrylics.com/products/pages/cassette.html >>> "Parker, Helayne" 5/3/2007 12:11 PM >>> Does anyone know where I can purchase those square brushed silver cassette holders that hold 15 empty cassettes (3 across in 5 rows). We use them when we set up the grosses on large specimens. We only have 3 and our Pathologist likes these organizers a lot. I can not find them to buy anywhere. Please advise. Thanks, Helayne Parker HT (ASCP) Histology Section Head Skaggs Community Health Center Branson, Missouri _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ccross6032 <@t> aol.com Thu May 3 14:29:00 2007 From: ccross6032 <@t> aol.com (Cheryl Cross) Date: Thu May 3 14:29:09 2007 Subject: [Histonet] anyone able to do double IHC staining for cox-2 and c-fos on mouse FFPE brain? Message-ID: hi everyone - I'm looking for a lab to run a double stain for cox-2 and c-fos on some FFPE mouse brains - anyone offering this? thanks! cheryl Cheryl Cross, DVM, Dipl. ACVP Researcher University Corporation for Atmospheric Research College of Veterinary Medicine University of Tennessee Department of Pathology 2407 River Drive, Room A201 Knoxville, TN 37996-4542 (423) 967-2724 fax: 865-974-5616 ccross@ucar.edu From mcauliff <@t> umdnj.edu Thu May 3 14:44:14 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu May 3 14:44:43 2007 Subject: [Histonet] storing paraformaldehyde under inert gas In-Reply-To: <20070502160639.megui4a1gq4ok80g@webmail.stanford.edu> References: <20070502160639.megui4a1gq4ok80g@webmail.stanford.edu> Message-ID: <463A3B8E.6010801@umdnj.edu> I have not heard of this and I just store my paraformaldehyde in its original container, tightly closed. I suppose you could buy a glove box, a closed chamber with gloves and inlet and outlet valves and use it to close the paraform. jar with nitrogen in the chamber. Seems like a lot of trouble ESPECIALLY since, if the lid is not 100% leak proof, you are wasting your time and money. Geoff wjtang@stanford.edu wrote: > Hi, > > We just ordered paraformaldehyde (crystalline form, Sigma P6148) and > found out from the MSDS that it needs to be stored under inert gas at > 4C. Does anyone know how to do store a reagent under inert gas? Or > does anyone do this at all w/ their paraformaldehyde? I tried > searching the Histonet archives and didn't find any info relating to > storing the solid but maybe I used the wrong search terms. > > Thanks in advance for any assistance! > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From pmarcum <@t> vet.upenn.edu Thu May 3 14:54:05 2007 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Thu May 3 14:54:10 2007 Subject: [Histonet] Need to Find These People Message-ID: <6.2.5.6.2.20070503154441.01fca430@vet.upenn.edu> Hi All, We are preparing for the Region 2 Fall Symposium in September, 2007. We have found the following people are missing or have e-mail, work and\or home addresses that are out of date. While these are or could be Maryland State Histology members we have them in a data base and would love to see them at the Rrgion Meeting. Could you please contact Gloria Limetti at glorialimetti@yahoo.com so we can update and send the information - PLEASE? Michelle Gitu Bradley Blumenauer Nancy Marinos Yvonne Chavez Tori Graves Reginald Woodard Kelly Benauer Joseph Madary Pratibhna Vohra Traci Sullivan Ashley Hull Elizabeth Smith Fred Argilan Elizabeth Williams Jerry Skwarek Annie Meriweather Mary Ann Cohen Dawn Spicer Linda Hrabek Gayle Andre Ravi Vohra Kimberly Tuttle Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From relia1 <@t> earthlink.net Thu May 3 14:54:57 2007 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu May 3 14:54:59 2007 Subject: [Histonet] RELIA's Histology Job Alert for 05/03/07 Message-ID: Hello Histonetters, I hope everybody is having a great week. These are brand new positions and I wanted to be the first to tell you about them. These are Full-Time Day Shift M-F permanent positions. My clients offer excellent compensation, benefits and relocation assistance. These clients have told me that they are Ready, Willing and Able to interview and make decisions quickly! Here are my latest job openings: Histo Tech - MN Histo Tech - OK Histo Tech - Northern CA I also have current openings for supervisors and techs in MA, TX, FL, VA, RI, IL and OH. If you are interested in any of these positions please contact me toll free at 866-607-3542 or by e-mail at relia1@earthlink.net Please feel free to pass my information on to others who might be interested. Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net From Barry.R.Rittman <@t> uth.tmc.edu Thu May 3 15:30:15 2007 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Thu May 3 15:30:18 2007 Subject: [Histonet] Paraformaldehyde. Message-ID: I agree totally with Geoff. While it sounds good - it is impractical to store most chemicals under inert gases. Would be nice to store dyes and stains so that they did not become oxidized etc. Might I suggest that you weigh out smalkl aliquots and place them in glass screw capped tubes and then keep these in a tightly sealed jar in the refrigerator. That shoudl solve the problem. If you are concerned about mixing then you might want to consider purchasing selaed glass vials of prepared paraformaldehyde solution. Not sure who sells that now but it used to be Ladd Industries.Glutaraldehye solutions in sealed vials also available. While these may seem expensive foe\r what they are - it does cut down on your time and effort and is safer than making solutions yourself. Additionally you could tell your supervisor that the most expensive item in the lab is (and should be) your time. Barry From asachau <@t> titanmed.com Thu May 3 15:59:16 2007 From: asachau <@t> titanmed.com (April Sachau) Date: Thu May 3 16:00:04 2007 Subject: [Histonet] Paraformaldehyde. In-Reply-To: Message-ID: <7E3ACD48BA6E26408F3188FBF08693F78B2B1A@titansbs1.corp.titanmed.com> Does anyone know if there is a similar net list to the "Histo Net" for Cyto and Med techs? From pruegg <@t> ihctech.net Thu May 3 16:37:21 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Thu May 3 16:37:26 2007 Subject: [Histonet] PSR In-Reply-To: Message-ID: <000301c78dcb$3ca3be30$6501a8c0@Patsy> When I did picro sirrus I rinsed well in dih20 to clear the yellow picric acid, then airdryed and coverslipped with permount. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Thursday, May 03, 2007 4:17 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSR Thanks Gail I will give it a go. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: Wednesday, May 02, 2007 2:51 PM To: Molinari, Betsy Subject: Re: [Histonet] PSR Betsty, After staining, try blotting the slide to dry, then coverslip without going through the alcohols, etc - just like van Giesons. Gayle Callis At 12:41 PM 5/2/2007, you wrote: >After coverslipping, my picro Sirius red slides show a leaching of what >seems to be the picric acid. I see a "halo" of yellow . I have tried >extending the acidified water rinses and followed w/ 100% ETOH - Xylene >. Any ideas? >Thanks! > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Thu May 3 16:37:21 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Thu May 3 16:37:30 2007 Subject: [Histonet] DAKO autostainer In-Reply-To: <11483605.1178207705544.JavaMail.root@fed1wml23.mgt.cox.net> Message-ID: <000701c78dcb$3e44eb10$6501a8c0@Patsy> I have used 4 different Dako Ihc stainers over the years and nothing really important has gone bad with any of them. I had a pump go out once but for me it just turned out to be a fuse replacement, didn't even need a new pump, I have heard about pumps going out but they are easy to fix, my husband in a mechanical engineer and he works on mine (don't tell Dako), I find them to be very reliable, open and easy to use with your own reagents. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of scorpionrider@cox.net Sent: Thursday, May 03, 2007 9:55 AM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] DAKO autostainer We have 4 stainers and they are great! The only thing that comes to mind when purchasing used equipment is whether your institution would rather spend the asdditional dollars and get new instruments with the accompanying warranties and service contracts, or are they willing to take a short-cut to save the bucks. Somtimes you can even work arrangements of an instrument in return for standing orders of reagents, depending upon your usage. Either way should work as these are good machines. Mark Turner, HT(ASCP) Mayo Clinic Arizona ---- "Weems wrote: > Same here! > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 404-851-7376 - Phone > 404-851-7831 - Fax > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jan > Shivers > Sent: Thursday, May 03, 2007 11:35 AM > To: Jo-Ann Bader, Ms.; Histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] DAKO autostainer > > > Jo-Ann, > > I have 2 DAKO autostainers and I would highly recommend them. Very easy to > program and utilize; versatile open system. > > Jan Shivers > Univ. of Minnesota Veterinary Diagnostic Lab > St. Paul, MN 55108 > shive003@umn.edu > > > ----- Original Message ----- > From: "Jo-Ann Bader, Ms." > To: > Sent: Thursday, May 03, 2007 8:39 AM > Subject: [Histonet] DAKO autostainer > > > > I have an offer to purchase a DAKO autostainer second hand. I know it has > not been used very much. The offer is for $40,000.00, half the purchase > price. I would appreciate hearing from those of you who have used one an > what you think of it. > > Thanks > Jo-Ann Bader > Histology Facility Coordinator > Molecular Oncology Group > MUHC/RVH > 687 Pine Ave. W - Rm. M11-53 > Montreal, QC, H3A-1A1 > Tel: 514-934-1934 Ext: 31780 > Fax: 514-843-1479 > email: jo-ann.bader@mcgill.ca > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DeBrosse_Beatrice <@t> Allergan.com Thu May 3 17:05:07 2007 From: DeBrosse_Beatrice <@t> Allergan.com (DeBrosse_Beatrice) Date: Thu May 3 17:05:37 2007 Subject: [Histonet] Silver Cassette Organizers Message-ID: Try the case management system from McCormick. Contact Misty Brown at StatLab 1-800-442-3453 x235. Beatrice DeBrosse-Serra HT(ASCP)QIHC Allergan, Inc. 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5116 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Parker, Helayne Sent: Thursday, May 03, 2007 9:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Silver Cassette Organizers Does anyone know where I can purchase those square brushed silver cassette holders that hold 15 empty cassettes (3 across in 5 rows). We use them when we set up the grosses on large specimens. We only have 3 and our Pathologist likes these organizers a lot. I can not find them to buy anywhere. Please advise. Thanks, Helayne Parker HT (ASCP) Histology Section Head Skaggs Community Health Center Branson, Missouri _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri May 4 01:50:03 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri May 4 01:50:08 2007 Subject: [Histonet] specimens recieved in formalin Message-ID: <86ADE4EB583CE64799A9924684A0FBBF01247673@wahtntex2.waht.swest.nhs.uk> I guess the problem is do you send out pre-filled formalin pots or do Staff apply the formalin to the specimen and pot elsewhere? The latter is dangerous and you risk inhalation and spillage problems with poorly trained Staff. The former is acceptable but you must have a spillage policy and you must have masks and a proper procedure in place for formalin use. Whether the area must be ventilated is a moot point and I think you need to risk assess the areas where the formalin and pots are stored; in the UK we own this risk as the Lab is the area of expertise concerning this matter and we have a duty of care; formalin monitoring may also be an option. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Wherever there is a human being, there is an opportunity for kindness. --Seneca This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From BMolinari <@t> heart.thi.tmc.edu Fri May 4 05:27:52 2007 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri May 4 05:27:58 2007 Subject: [Histonet] PSR In-Reply-To: <000301c78dcb$3ca3be30$6501a8c0@Patsy> Message-ID: Thank you Patsy and to all who helped me. I am a bit chagrined that I did not take the obvious route of simply reading the bottle. A good reminder that sometimes the answers are right under our noses..it is just too easy to go to the Histonetters.Have a good weekend. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: Patsy Ruegg [mailto:pruegg@ihctech.net] Sent: Thursday, May 03, 2007 4:37 PM To: Molinari, Betsy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSR When I did picro sirrus I rinsed well in dih20 to clear the yellow picric acid, then airdryed and coverslipped with permount. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Thursday, May 03, 2007 4:17 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSR Thanks Gail I will give it a go. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: Wednesday, May 02, 2007 2:51 PM To: Molinari, Betsy Subject: Re: [Histonet] PSR Betsty, After staining, try blotting the slide to dry, then coverslip without going through the alcohols, etc - just like van Giesons. Gayle Callis At 12:41 PM 5/2/2007, you wrote: >After coverslipping, my picro Sirius red slides show a leaching of what >seems to be the picric acid. I see a "halo" of yellow . I have tried >extending the acidified water rinses and followed w/ 100% ETOH - Xylene >. Any ideas? >Thanks! > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From atouvr <@t> yahoo.com Fri May 4 05:55:25 2007 From: atouvr <@t> yahoo.com (annamaria touvra) Date: Fri May 4 05:55:36 2007 Subject: [Histonet] Re: Histonet Digest, Vol 42, Issue 2 Message-ID: <821457.16160.qm@web60718.mail.yahoo.com> Hello There! I am interested too for this article. Any protocols are welcome, Thank you, Anna-Maria Nicosia, RF and Ottinetti A. Modulation of microvascular growth and morphogenesis by reconstituted basement membrane gel in three dimensional cultures of rat aorta: a comparative study of angiogenesis in matrigel, collagen, fibrin, and plasma clot. In Vitro Cell Dev Biol. 1990 Feb;26(2):119-28. histonet-request@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. California Society for Histotechnology Spring Meeting May 17-20 (Morken, Tim) 2. Cell Button Prep (Heckford, Karen - SMMC-SF) 3. bone marrow smears (path lab) 4. Fatty tissue (Jennifer Johnson) 5. 2 F/T Permanent Histology Bench and Histo Supv positions in SW Texas & East Texas (Eric Dye (ext 223)) 6. peloris? (connie grubaugh) 7. Certified thermometers (WWmn916@aol.com) 8. RE: Cell Button Prep (Kemlo Rogerson) 9. osteoclast/ osteoblast markers (louise renton) 10. CD52 (Doug Geddes) 11. alcian blue_precipitaion_solution (raghul) 12. Re: Fatty tissue (Rene J Buesa) 13. MOHS (April Sachau) 14. Re: Certified thermometers (Rene J Buesa) 15. Re: MOHS (Rene J Buesa) 16. looking for Freida Carson (godsgalnow@aol.com) 17. RE: Certified thermometers (Kemlo Rogerson) 18. RE: Certified thermometers (Rene J Buesa) 19. RE: Certified thermometers (soofia siddiqui) 20. Aortic ring references (Andrea T. Hooper) ---------------------------------------------------------------------- Message: 1 Date: Tue, 1 May 2007 14:22:46 -0400 From: "Morken, Tim" Subject: [Histonet] California Society for Histotechnology Spring Meeting May 17-20 To: "histonet" Message-ID: <6BFF6D137DF6BC43B33891BA96E83B1959B5DD@PGHCR-EXMB-VS-1.na.fshrnet.com> Content-Type: text/plain; charset="us-ascii" Information and registration forms for the California Society for Histotechnology Spring Meeting on May 17-20 in San Mateo (near San Jose) can be downloaded at the California society website: www.californiahistology.org Tim Morken ------------------------------ Message: 2 Date: Tue, 1 May 2007 12:51:36 -0700 From: "Heckford, Karen - SMMC-SF" Subject: [Histonet] Cell Button Prep To: "Histonet (histonet@lists.utsouthwestern.edu)" Message-ID: Content-Type: text/plain; charset="us-ascii" I was wondering if anyone would be so kind as giving input on the different ways of preparing a cell button. We work a lot with Pleural Fluid and Ascites Fluid. I have been told two different ways. One is just 1/2 and 1/2 with fluid and 95%alcohol and spend down decant and get your cell button. The other is spend down the fluid decant add 10%formalin respin and let set then get your cell button. Of course this all after I have made my cytospins. We do not have a ThinPrep type machine. Any input would be very appreciative. I realize there is probably more ways to do this that I have not heard of. Cheers, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathology Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu ------------------------------ Message: 3 Date: Tue, 1 May 2007 16:24:42 -0400 From: "path lab" Subject: [Histonet] bone marrow smears To: histonet@lists.utsouthwestern.edu Message-ID: <73841640705011324w79dfab34u57e8553b37923836@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Could anyone please provide a procedure for bone marrow smears from necropsy to staining with Wrights-Giemsa? Thanks for your help in advance. ------------------------------ Message: 4 Date: Tue, 1 May 2007 16:55:49 -0400 From: "Jennifer Johnson" Subject: [Histonet] Fatty tissue To: Message-ID: <000701c78c33$1eb04e00$2082010a@main.crispregional.org> Content-Type: text/plain; charset="us-ascii" Does anyone have any suggestions for cutting better frozen sections of very fatty tissue (breast in particular)? When I turn the cryostat colder, my Pathologist complains about the artifact on the permanent sections. Any help is appreciated. Thanks, Jennifer ------------------------------ Message: 5 Date: Tue, 1 May 2007 18:18:17 -0400 From: Eric Dye (ext 223) Subject: [Histonet] 2 F/T Permanent Histology Bench and Histo Supv positions in SW Texas & East Texas To: Histonetters Message-ID: Content-Type: text/plain Hi - Histonetters -Are you still working at ? I am presently on a search for several of my clients in Texas who are looking to hire HistoTech employees. I have Two(2) F/T Permanent Histology positions in Texas.BOTH positions are perm F/T as direct employees of my clients. They are regular employee positions that offer you 40hrs/wk with full employe benfits - including: 401-K, Full Health Insur., Vacation/Sick/Holiday & Full Relo. Permanent Histology positions in Texas 1. S.W. Texas - Bench Histo Tech - Mon - Fri Days - work schedule is flexible to meet YOUR needs. 2. East Texas - HistoTech Supervisor - Mon - Fri Days - 8a -5pm 3. East Texas - Bench Histo Tech Supervisor - Mon - Fri Days - hours flex to suit your needs. If you are interested in any of the HISTOTECH J0BS listed above - please call me at 800-466-9919 ext 223 or cell - 407-756-5507...OR .. May I call you at or to discuss these Histo Tech openings? Thank you, Eric Dye-Sr Allied Healthcare Recruiter 800-466-9919 ext 223 or Cell - 407-756-5507 P.S.: Feel free to pass along this email and my phone number to anyone who you think might be interested. P.S.S.: The clients are currently interviewing - and the positions will close soon - so if you are interested, please call me today at 1-800-466-9919 ext 223 --------------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing 800-466-9919 ext 223 - voice 407 671 6075 - fax eric@ategra.com To Learn More About Ategra: http://www.ategra.com ---------------------------------------------------------------- If you received this by mistake, or if you wish not to hear from me, please shoot me a mail to let me know and I'll not mail you again. ---------------------------------------------------------------- ---------------------------------------------------------------- Note: this message is intended for: Histonetters at histonet@lists.utsouthwestern.edu ---------------------------------------------------------------- ------------------------------ Message: 6 Date: Wed, 2 May 2007 03:19:58 +0000 From: connie grubaugh Subject: [Histonet] peloris? To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="Windows-1252" In the market for a new processor and would like feed back on the Peloris good and bad from anyone that has this machine. Connie G. _________________________________________________________________ Discover the new Windows Vista http://search.msn.com/results.aspx?q=windows+vista&mkt=en-US&form=QBRE ------------------------------ Message: 7 Date: Tue, 1 May 2007 23:33:21 EDT From: WWmn916@aol.com Subject: [Histonet] Certified thermometers To: histonet@pathology.swmed.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Hello everyone, I'm hoping for a little help. What is everyone using to calibrate routine thermometers in the histology lab (thermometers for refrig, H20 baths, IPOX machines....etc.)? We can no longer use mercury filled certified thermometers to test the others. Any suggestions? How often do the certified thermometers need to be recalibrated? Thanks much, Deb King California ************************************** See what's free at http://www.aol.com. ------------------------------ Message: 8 Date: Wed, 2 May 2007 08:11:00 +0100 From: "Kemlo Rogerson" Subject: RE: [Histonet] Cell Button Prep To: "Heckford, Karen - SMMC-SF" , Message-ID: <86ADE4EB583CE64799A9924684A0FBBF01247645@wahtntex2.waht.swest.nhs.uk> Content-Type: text/plain; charset="us-ascii" You could try the hypaque method. If you are interested I could get the method but it bluntly uses a density gradient. You spin down the fluid remove supernatant and reconstitute with saline. You make a solution of hypaque and layer the cell/ saline solution on top. You then spin, rbcs and crude fall through the hypaque and larger cells float on top of the hypaque. Harvest these cells, wash and then mix with agar and cool. Process and cut and stain. Job done. But ThinPrep is the way forward from my experience and if you are clever you can come up with some sedimentation process that might emulate ThinPrep similar to the one SurgiPath was flogging; I think it was French but god knows what happened to it. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; We need to be open to the possibility that colleagues and even strangers have information and perspectives that may be of value to us. --Margaret Wheatley This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation ------------------------------ Message: 9 Date: Wed, 2 May 2007 10:38:18 +0200 From: "louise renton" Subject: [Histonet] osteoclast/ osteoblast markers To: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hi all, what recommendations for the most specific immuno markers for osteoclasts, osteoblasts and macrophages in FFPE primate/human tissue? Anybody using CD163 and what have your results been like Thanks in advance best regards -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ------------------------------ Message: 10 Date: Wed, 02 May 2007 08:04:42 -0400 From: "Doug Geddes" Subject: [Histonet] CD52 To: Message-ID: <4638461A0200006100001DF6@lhscgwiao.lhsc.on.ca> Content-Type: text/plain; charset=US-ASCII Looking for a reliable supplier of CD52 for formalin fixed paraffin embedded tissue, and any methods. Doug Geddes BSc, MLT Department of Pathology London Helath Sciences Centre London, ON Canada ------------------------------ Message: 11 Date: Wed, 2 May 2007 14:32:15 +0100 (BST) From: raghul Subject: [Histonet] alcian blue_precipitaion_solution To: histonet@lists.utsouthwestern.edu Message-ID: <681959.20532.qm@web8322.mail.in.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 dear histonetters hope atleast the persons remember my problem of alcian blue(sigma) precpitating in dis. water. I went on to stirr for 2 hours followed by boiling for 5 min. I could make a 2.5ph solution of alcian blue and its staining well. I think boiling was the main reason. thanks for all those suggestions raghul medclone biotech chennai, india --------------------------------- Check out what you're missing if you're not on Yahoo! Messenger ------------------------------ Message: 12 Date: Wed, 2 May 2007 06:40:26 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Fatty tissue To: Jennifer Johnson , histonet@lists.utsouthwestern.edu Message-ID: <885157.50026.qm@web61219.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Deep freeze quickly, cut slowly, increase thickness "just a little bit" and use the "anti-rolling" devise (of a camel hair brush) to "pull" the section. Ren? J. Jennifer Johnson wrote: Does anyone have any suggestions for cutting better frozen sections of very fatty tissue (breast in particular)? When I turn the cryostat colder, my Pathologist complains about the artifact on the permanent sections. Any help is appreciated. Thanks, Jennifer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. ------------------------------ Message: 13 Date: Wed, 2 May 2007 08:40:32 -0500 From: "April Sachau" Subject: [Histonet] MOHS To: "raghul" , Message-ID: <7E3ACD48BA6E26408F3188FBF08693F78B246F@titansbs1.corp.titanmed.com> Content-Type: text/plain; charset="us-ascii" Good Morning, Can anyone please tell me why it has been impossible to find someone with strong MOHS experience? April Sachau Titan Medical Group Staff Supervisor Phone (866) 332-9600 Ext. 1023 Fax (402) 332-5181 asachau@titanmed.com see us on the web at www.titanmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of raghul Sent: Wednesday, May 02, 2007 8:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] alcian blue_precipitaion_solution dear histonetters hope atleast the persons remember my problem of alcian blue(sigma) precpitating in dis. water. I went on to stirr for 2 hours followed by boiling for 5 min. I could make a 2.5ph solution of alcian blue and its staining well. I think boiling was the main reason. thanks for all those suggestions raghul medclone biotech chennai, india --------------------------------- Check out what you're missing if you're not on Yahoo! Messenger _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Wed, 2 May 2007 06:50:36 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Certified thermometers To: WWmn916@aol.com, histonet@pathology.swmed.edu Message-ID: <872229.17519.qm@web61224.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Calibrate them yourself! Just place your thermometer in boilong water and, if you are at sea level and there is no atmosferic low pressure or metheorological disturbance around, your thermometer should read 100?C when the water starts to boil. If there is a different reading (either higher or lower) that difference will be your thermometer correction, meaning that to whatever reading you have, you will have to add (+) or substract (-) that difference. For 0?C fill a container with distilled water and add ice cubes and, with the same provisions as before, the reading should be 0?C when there is a surface ice crust of ice in the water + ice. Once a year is enough OR you could buy a digital thermometer (for less that $35). ALL come certified and avoid all the hassle previously described! Ren? J. WWmn916@aol.com wrote: Hello everyone, I'm hoping for a little help. What is everyone using to calibrate routine thermometers in the histology lab (thermometers for refrig, H20 baths, IPOX machines....etc.)? We can no longer use mercury filled certified thermometers to test the others. Any suggestions? How often do the certified thermometers need to be recalibrated? Thanks much, Deb King California ************************************** See what's free at http://www.aol.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. ------------------------------ Message: 15 Date: Wed, 2 May 2007 07:03:17 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] MOHS To: April Sachau , raghul , histonet@lists.utsouthwestern.edu Message-ID: <825625.80927.qm@web61214.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 In the last issue of Advance MLP comes an address that you could try: www.mohshistotemp.com Ren? J. April Sachau wrote: Good Morning, Can anyone please tell me why it has been impossible to find someone with strong MOHS experience? April Sachau Titan Medical Group Staff Supervisor Phone (866) 332-9600 Ext. 1023 Fax (402) 332-5181 asachau@titanmed.com see us on the web at www.titanmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of raghul Sent: Wednesday, May 02, 2007 8:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] alcian blue_precipitaion_solution dear histonetters hope atleast the persons remember my problem of alcian blue(sigma) precpitating in dis. water. I went on to stirr for 2 hours followed by boiling for 5 min. I could make a 2.5ph solution of alcian blue and its staining well. I think boiling was the main reason. thanks for all those suggestions raghul medclone biotech chennai, india --------------------------------- Check out what you're missing if you're not on Yahoo! Messenger _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. ------------------------------ Message: 16 Date: Wed, 02 May 2007 10:05:14 -0400 From: godsgalnow@aol.com Subject: [Histonet] looking for Freida Carson To: histonet@lists.utsouthwestern.edu Message-ID: <8C95ADA0A9C8D49-D0C-449A@FWM-R13.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" I am looking for Freida...can you please email me. Thanks, Roxanne Soto HT(ASCP)QIHC ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. ------------------------------ Message: 17 Date: Wed, 2 May 2007 15:59:29 +0100 From: "Kemlo Rogerson" Subject: RE: [Histonet] Certified thermometers To: "Rene J Buesa" , , Message-ID: <86ADE4EB583CE64799A9924684A0FBBF01247657@wahtntex2.waht.swest.nhs.uk> Content-Type: text/plain; charset="Windows-1252" your thermometer should read 100?C when the water starts to boil. Rene If water boils longer does it get hotter? I thought water boiled at 100 degrees however long you left it; if you increased the heat it still boiled at 100 degrees but the increased latent heat of vaporisation meant that more water was able to vaporise but still only at 100 degrees. Kemlo For 0?C fill a container with distilled water and add ice cubes and, with the same provisions as before, the reading should be 0?C when there is a surface ice crust of ice in the water + ice. Rene Same with freezing; I thought water could be 'frozen' at the eutectic point of water which is +4 degrees C (that's how you get 'black ice'). It certainly is at its most dense (which is why ice floats) and wouldn't the bottom of the water be hotter than at the top cos the coldest water floats (its less dense at the eutectic point) that's why we are here are were able to crawl out of the sea and my Koi don't freeze in Winter. Never really understood water that why I used to brew beer in it. I'd buy a digital thermometer because those of you at sea level will soon drown anyway with global warming. === message truncated === ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Anna-Maria Touvra MSc Democritus University of Thrace Department of Physical Education and Sport Science Address: University Campus, TEFAA Komotini 69100, GREECE Tel: 00306938511677, 00302531030343 Contact information in Finland Address: Haperontie 1B 31, 40640 JYV?SKYL?, FINLAND Tel: 00358509324688 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From atouvr <@t> yahoo.com Fri May 4 05:55:52 2007 From: atouvr <@t> yahoo.com (annamaria touvra) Date: Fri May 4 05:55:57 2007 Subject: [Histonet] Re: Histonet Digest, Vol 42, Issue 2 Message-ID: <217174.86918.qm@web60722.mail.yahoo.com> Hello There! I am interested too for this article. Any protocols are welcome, Thank you, Anna-Maria Nicosia, RF and Ottinetti A. Modulation of microvascular growth and morphogenesis by reconstituted basement membrane gel in three dimensional cultures of rat aorta: a comparative study of angiogenesis in matrigel, collagen, fibrin, and plasma clot. In Vitro Cell Dev Biol. 1990 Feb;26(2):119-28. histonet-request@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. California Society for Histotechnology Spring Meeting May 17-20 (Morken, Tim) 2. Cell Button Prep (Heckford, Karen - SMMC-SF) 3. bone marrow smears (path lab) 4. Fatty tissue (Jennifer Johnson) 5. 2 F/T Permanent Histology Bench and Histo Supv positions in SW Texas & East Texas (Eric Dye (ext 223)) 6. peloris? (connie grubaugh) 7. Certified thermometers (WWmn916@aol.com) 8. RE: Cell Button Prep (Kemlo Rogerson) 9. osteoclast/ osteoblast markers (louise renton) 10. CD52 (Doug Geddes) 11. alcian blue_precipitaion_solution (raghul) 12. Re: Fatty tissue (Rene J Buesa) 13. MOHS (April Sachau) 14. Re: Certified thermometers (Rene J Buesa) 15. Re: MOHS (Rene J Buesa) 16. looking for Freida Carson (godsgalnow@aol.com) 17. RE: Certified thermometers (Kemlo Rogerson) 18. RE: Certified thermometers (Rene J Buesa) 19. RE: Certified thermometers (soofia siddiqui) 20. Aortic ring references (Andrea T. Hooper) ---------------------------------------------------------------------- Message: 1 Date: Tue, 1 May 2007 14:22:46 -0400 From: "Morken, Tim" Subject: [Histonet] California Society for Histotechnology Spring Meeting May 17-20 To: "histonet" Message-ID: <6BFF6D137DF6BC43B33891BA96E83B1959B5DD@PGHCR-EXMB-VS-1.na.fshrnet.com> Content-Type: text/plain; charset="us-ascii" Information and registration forms for the California Society for Histotechnology Spring Meeting on May 17-20 in San Mateo (near San Jose) can be downloaded at the California society website: www.californiahistology.org Tim Morken ------------------------------ Message: 2 Date: Tue, 1 May 2007 12:51:36 -0700 From: "Heckford, Karen - SMMC-SF" Subject: [Histonet] Cell Button Prep To: "Histonet (histonet@lists.utsouthwestern.edu)" Message-ID: Content-Type: text/plain; charset="us-ascii" I was wondering if anyone would be so kind as giving input on the different ways of preparing a cell button. We work a lot with Pleural Fluid and Ascites Fluid. I have been told two different ways. One is just 1/2 and 1/2 with fluid and 95%alcohol and spend down decant and get your cell button. The other is spend down the fluid decant add 10%formalin respin and let set then get your cell button. Of course this all after I have made my cytospins. We do not have a ThinPrep type machine. Any input would be very appreciative. I realize there is probably more ways to do this that I have not heard of. Cheers, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathology Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu ------------------------------ Message: 3 Date: Tue, 1 May 2007 16:24:42 -0400 From: "path lab" Subject: [Histonet] bone marrow smears To: histonet@lists.utsouthwestern.edu Message-ID: <73841640705011324w79dfab34u57e8553b37923836@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Could anyone please provide a procedure for bone marrow smears from necropsy to staining with Wrights-Giemsa? Thanks for your help in advance. ------------------------------ Message: 4 Date: Tue, 1 May 2007 16:55:49 -0400 From: "Jennifer Johnson" Subject: [Histonet] Fatty tissue To: Message-ID: <000701c78c33$1eb04e00$2082010a@main.crispregional.org> Content-Type: text/plain; charset="us-ascii" Does anyone have any suggestions for cutting better frozen sections of very fatty tissue (breast in particular)? When I turn the cryostat colder, my Pathologist complains about the artifact on the permanent sections. Any help is appreciated. Thanks, Jennifer ------------------------------ Message: 5 Date: Tue, 1 May 2007 18:18:17 -0400 From: Eric Dye (ext 223) Subject: [Histonet] 2 F/T Permanent Histology Bench and Histo Supv positions in SW Texas & East Texas To: Histonetters Message-ID: Content-Type: text/plain Hi - Histonetters -Are you still working at ? I am presently on a search for several of my clients in Texas who are looking to hire HistoTech employees. I have Two(2) F/T Permanent Histology positions in Texas.BOTH positions are perm F/T as direct employees of my clients. They are regular employee positions that offer you 40hrs/wk with full employe benfits - including: 401-K, Full Health Insur., Vacation/Sick/Holiday & Full Relo. Permanent Histology positions in Texas 1. S.W. Texas - Bench Histo Tech - Mon - Fri Days - work schedule is flexible to meet YOUR needs. 2. East Texas - HistoTech Supervisor - Mon - Fri Days - 8a -5pm 3. East Texas - Bench Histo Tech Supervisor - Mon - Fri Days - hours flex to suit your needs. If you are interested in any of the HISTOTECH J0BS listed above - please call me at 800-466-9919 ext 223 or cell - 407-756-5507...OR .. May I call you at or to discuss these Histo Tech openings? Thank you, Eric Dye-Sr Allied Healthcare Recruiter 800-466-9919 ext 223 or Cell - 407-756-5507 P.S.: Feel free to pass along this email and my phone number to anyone who you think might be interested. P.S.S.: The clients are currently interviewing - and the positions will close soon - so if you are interested, please call me today at 1-800-466-9919 ext 223 --------------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing 800-466-9919 ext 223 - voice 407 671 6075 - fax eric@ategra.com To Learn More About Ategra: http://www.ategra.com ---------------------------------------------------------------- If you received this by mistake, or if you wish not to hear from me, please shoot me a mail to let me know and I'll not mail you again. ---------------------------------------------------------------- ---------------------------------------------------------------- Note: this message is intended for: Histonetters at histonet@lists.utsouthwestern.edu ---------------------------------------------------------------- ------------------------------ Message: 6 Date: Wed, 2 May 2007 03:19:58 +0000 From: connie grubaugh Subject: [Histonet] peloris? To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="Windows-1252" In the market for a new processor and would like feed back on the Peloris good and bad from anyone that has this machine. Connie G. _________________________________________________________________ Discover the new Windows Vista http://search.msn.com/results.aspx?q=windows+vista&mkt=en-US&form=QBRE ------------------------------ Message: 7 Date: Tue, 1 May 2007 23:33:21 EDT From: WWmn916@aol.com Subject: [Histonet] Certified thermometers To: histonet@pathology.swmed.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Hello everyone, I'm hoping for a little help. What is everyone using to calibrate routine thermometers in the histology lab (thermometers for refrig, H20 baths, IPOX machines....etc.)? We can no longer use mercury filled certified thermometers to test the others. Any suggestions? How often do the certified thermometers need to be recalibrated? Thanks much, Deb King California ************************************** See what's free at http://www.aol.com. ------------------------------ Message: 8 Date: Wed, 2 May 2007 08:11:00 +0100 From: "Kemlo Rogerson" Subject: RE: [Histonet] Cell Button Prep To: "Heckford, Karen - SMMC-SF" , Message-ID: <86ADE4EB583CE64799A9924684A0FBBF01247645@wahtntex2.waht.swest.nhs.uk> Content-Type: text/plain; charset="us-ascii" You could try the hypaque method. If you are interested I could get the method but it bluntly uses a density gradient. You spin down the fluid remove supernatant and reconstitute with saline. You make a solution of hypaque and layer the cell/ saline solution on top. You then spin, rbcs and crude fall through the hypaque and larger cells float on top of the hypaque. Harvest these cells, wash and then mix with agar and cool. Process and cut and stain. Job done. But ThinPrep is the way forward from my experience and if you are clever you can come up with some sedimentation process that might emulate ThinPrep similar to the one SurgiPath was flogging; I think it was French but god knows what happened to it. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; We need to be open to the possibility that colleagues and even strangers have information and perspectives that may be of value to us. --Margaret Wheatley This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation ------------------------------ Message: 9 Date: Wed, 2 May 2007 10:38:18 +0200 From: "louise renton" Subject: [Histonet] osteoclast/ osteoblast markers To: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hi all, what recommendations for the most specific immuno markers for osteoclasts, osteoblasts and macrophages in FFPE primate/human tissue? Anybody using CD163 and what have your results been like Thanks in advance best regards -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ------------------------------ Message: 10 Date: Wed, 02 May 2007 08:04:42 -0400 From: "Doug Geddes" Subject: [Histonet] CD52 To: Message-ID: <4638461A0200006100001DF6@lhscgwiao.lhsc.on.ca> Content-Type: text/plain; charset=US-ASCII Looking for a reliable supplier of CD52 for formalin fixed paraffin embedded tissue, and any methods. Doug Geddes BSc, MLT Department of Pathology London Helath Sciences Centre London, ON Canada ------------------------------ Message: 11 Date: Wed, 2 May 2007 14:32:15 +0100 (BST) From: raghul Subject: [Histonet] alcian blue_precipitaion_solution To: histonet@lists.utsouthwestern.edu Message-ID: <681959.20532.qm@web8322.mail.in.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 dear histonetters hope atleast the persons remember my problem of alcian blue(sigma) precpitating in dis. water. I went on to stirr for 2 hours followed by boiling for 5 min. I could make a 2.5ph solution of alcian blue and its staining well. I think boiling was the main reason. thanks for all those suggestions raghul medclone biotech chennai, india --------------------------------- Check out what you're missing if you're not on Yahoo! Messenger ------------------------------ Message: 12 Date: Wed, 2 May 2007 06:40:26 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Fatty tissue To: Jennifer Johnson , histonet@lists.utsouthwestern.edu Message-ID: <885157.50026.qm@web61219.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Deep freeze quickly, cut slowly, increase thickness "just a little bit" and use the "anti-rolling" devise (of a camel hair brush) to "pull" the section. Ren? J. Jennifer Johnson wrote: Does anyone have any suggestions for cutting better frozen sections of very fatty tissue (breast in particular)? When I turn the cryostat colder, my Pathologist complains about the artifact on the permanent sections. Any help is appreciated. Thanks, Jennifer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. ------------------------------ Message: 13 Date: Wed, 2 May 2007 08:40:32 -0500 From: "April Sachau" Subject: [Histonet] MOHS To: "raghul" , Message-ID: <7E3ACD48BA6E26408F3188FBF08693F78B246F@titansbs1.corp.titanmed.com> Content-Type: text/plain; charset="us-ascii" Good Morning, Can anyone please tell me why it has been impossible to find someone with strong MOHS experience? April Sachau Titan Medical Group Staff Supervisor Phone (866) 332-9600 Ext. 1023 Fax (402) 332-5181 asachau@titanmed.com see us on the web at www.titanmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of raghul Sent: Wednesday, May 02, 2007 8:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] alcian blue_precipitaion_solution dear histonetters hope atleast the persons remember my problem of alcian blue(sigma) precpitating in dis. water. I went on to stirr for 2 hours followed by boiling for 5 min. I could make a 2.5ph solution of alcian blue and its staining well. I think boiling was the main reason. thanks for all those suggestions raghul medclone biotech chennai, india --------------------------------- Check out what you're missing if you're not on Yahoo! Messenger _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Wed, 2 May 2007 06:50:36 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Certified thermometers To: WWmn916@aol.com, histonet@pathology.swmed.edu Message-ID: <872229.17519.qm@web61224.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Calibrate them yourself! Just place your thermometer in boilong water and, if you are at sea level and there is no atmosferic low pressure or metheorological disturbance around, your thermometer should read 100?C when the water starts to boil. If there is a different reading (either higher or lower) that difference will be your thermometer correction, meaning that to whatever reading you have, you will have to add (+) or substract (-) that difference. For 0?C fill a container with distilled water and add ice cubes and, with the same provisions as before, the reading should be 0?C when there is a surface ice crust of ice in the water + ice. Once a year is enough OR you could buy a digital thermometer (for less that $35). ALL come certified and avoid all the hassle previously described! Ren? J. WWmn916@aol.com wrote: Hello everyone, I'm hoping for a little help. What is everyone using to calibrate routine thermometers in the histology lab (thermometers for refrig, H20 baths, IPOX machines....etc.)? We can no longer use mercury filled certified thermometers to test the others. Any suggestions? How often do the certified thermometers need to be recalibrated? Thanks much, Deb King California ************************************** See what's free at http://www.aol.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. ------------------------------ Message: 15 Date: Wed, 2 May 2007 07:03:17 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] MOHS To: April Sachau , raghul , histonet@lists.utsouthwestern.edu Message-ID: <825625.80927.qm@web61214.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 In the last issue of Advance MLP comes an address that you could try: www.mohshistotemp.com Ren? J. April Sachau wrote: Good Morning, Can anyone please tell me why it has been impossible to find someone with strong MOHS experience? April Sachau Titan Medical Group Staff Supervisor Phone (866) 332-9600 Ext. 1023 Fax (402) 332-5181 asachau@titanmed.com see us on the web at www.titanmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of raghul Sent: Wednesday, May 02, 2007 8:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] alcian blue_precipitaion_solution dear histonetters hope atleast the persons remember my problem of alcian blue(sigma) precpitating in dis. water. I went on to stirr for 2 hours followed by boiling for 5 min. I could make a 2.5ph solution of alcian blue and its staining well. I think boiling was the main reason. thanks for all those suggestions raghul medclone biotech chennai, india --------------------------------- Check out what you're missing if you're not on Yahoo! Messenger _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. ------------------------------ Message: 16 Date: Wed, 02 May 2007 10:05:14 -0400 From: godsgalnow@aol.com Subject: [Histonet] looking for Freida Carson To: histonet@lists.utsouthwestern.edu Message-ID: <8C95ADA0A9C8D49-D0C-449A@FWM-R13.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" I am looking for Freida...can you please email me. Thanks, Roxanne Soto HT(ASCP)QIHC ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. ------------------------------ Message: 17 Date: Wed, 2 May 2007 15:59:29 +0100 From: "Kemlo Rogerson" Subject: RE: [Histonet] Certified thermometers To: "Rene J Buesa" , , Message-ID: <86ADE4EB583CE64799A9924684A0FBBF01247657@wahtntex2.waht.swest.nhs.uk> Content-Type: text/plain; charset="Windows-1252" your thermometer should read 100?C when the water starts to boil. Rene If water boils longer does it get hotter? I thought water boiled at 100 degrees however long you left it; if you increased the heat it still boiled at 100 degrees but the increased latent heat of vaporisation meant that more water was able to vaporise but still only at 100 degrees. Kemlo For 0?C fill a container with distilled water and add ice cubes and, with the same provisions as before, the reading should be 0?C when there is a surface ice crust of ice in the water + ice. Rene Same with freezing; I thought water could be 'frozen' at the eutectic point of water which is +4 degrees C (that's how you get 'black ice'). It certainly is at its most dense (which is why ice floats) and wouldn't the bottom of the water be hotter than at the top cos the coldest water floats (its less dense at the eutectic point) that's why we are here are were able to crawl out of the sea and my Koi don't freeze in Winter. Never really understood water that why I used to brew beer in it. I'd buy a digital thermometer because those of you at sea level will soon drown anyway with global warming. === message truncated === ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Anna-Maria Touvra MSc Democritus University of Thrace Department of Physical Education and Sport Science Address: University Campus, TEFAA Komotini 69100, GREECE Tel: 00306938511677, 00302531030343 Contact information in Finland Address: Haperontie 1B 31, 40640 JYV?SKYL?, FINLAND Tel: 00358509324688 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ --------------------------------- Sucker-punch spam with award-winning protection. Try the free Yahoo! Mail Beta. From jjohnson <@t> crispregional.org Fri May 4 08:17:04 2007 From: jjohnson <@t> crispregional.org (Jennifer Johnson) Date: Fri May 4 08:19:53 2007 Subject: [Histonet] BTA Message-ID: <000001c78e4e$86c6fe90$2082010a@main.crispregional.org> Is anyone currently doing BTA TRAC & BTA STAT? We currently send out all immunos to a reference lab and wanted to know if these specimens were acceptable if admixed with Saccomanno fixative. From BrealK <@t> alexian.net Fri May 4 09:50:40 2007 From: BrealK <@t> alexian.net (Kari Breal) Date: Fri May 4 09:51:39 2007 Subject: [Histonet] Receiving all placentas Message-ID: Our OB/GYN department is requesting we accession/receive all placentas. They want us to continue with the gross and micro for the one that meet the criteria for examination. For the normal placentas, they want us to gross the placenta, take a few sections and store these sections (uncut) for up to 20 years. Is there any pathology departments that receive all placentas? If you do, do you perform just a gross exam on the normal placentas or do you perform a gross and micro on all specimens? If you receive all placentas, do the insurance companies reimburse for the normal (the ones that do not meet the CAP guidelines for examination) placentas? Please contact me if your hospital does this or something similar with the placentas. Thank you, Kari Breal Histology Supervisor Alexian Brothers Medical Center 847-437-5500 ext. 5155 Fax 847-981-2023 brealk@alexian.net CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From jnocito <@t> satx.rr.com Fri May 4 09:58:07 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri May 4 09:58:08 2007 Subject: [Histonet] DAKO autostainer References: <000701c78dcb$3e44eb10$6501a8c0@Patsy> Message-ID: <013b01c7d44c$584776f0$d49eae18@yourxhtr8hvc4p> I refuse to answer on the grounds it may incriminate me again and again. JTT ----- Original Message ----- From: "Patsy Ruegg" To: ; Sent: Thursday, May 03, 2007 4:37 PM Subject: RE: [Histonet] DAKO autostainer >I have used 4 different Dako Ihc stainers over the years and nothing really > important has gone bad with any of them. I had a pump go out once but for > me it just turned out to be a fuse replacement, didn't even need a new > pump, > I have heard about pumps going out but they are easy to fix, my husband in > a > mechanical engineer and he works on mine (don't tell Dako), I find them to > be very reliable, open and easy to use with your own reagents. > Patsy > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > scorpionrider@cox.net > Sent: Thursday, May 03, 2007 9:55 AM > To: Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] DAKO autostainer > > > We have 4 stainers and they are great! The only thing that comes to mind > when purchasing used equipment is whether your institution would rather > spend the asdditional dollars and get new instruments with the > accompanying > warranties and service contracts, or are they willing to take a short-cut > to > save the bucks. Somtimes you can even work arrangements of an instrument > in > return for standing orders of reagents, depending upon your usage. Either > way should work as these are good machines. > Mark Turner, HT(ASCP) > Mayo Clinic Arizona > > > ---- "Weems wrote: >> Same here! >> >> Joyce Weems >> Pathology Manager >> Saint Joseph's Hospital >> 5665 Peachtree Dunwoody Rd NE >> Atlanta, GA 30342 >> 404-851-7376 - Phone >> 404-851-7831 - Fax >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jan >> Shivers >> Sent: Thursday, May 03, 2007 11:35 AM >> To: Jo-Ann Bader, Ms.; Histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] DAKO autostainer >> >> >> Jo-Ann, >> >> I have 2 DAKO autostainers and I would highly recommend them. Very easy >> to >> program and utilize; versatile open system. >> >> Jan Shivers >> Univ. of Minnesota Veterinary Diagnostic Lab >> St. Paul, MN 55108 >> shive003@umn.edu >> >> >> ----- Original Message ----- >> From: "Jo-Ann Bader, Ms." >> To: >> Sent: Thursday, May 03, 2007 8:39 AM >> Subject: [Histonet] DAKO autostainer >> >> >> >> I have an offer to purchase a DAKO autostainer second hand. I know it has >> not been used very much. The offer is for $40,000.00, half the purchase >> price. I would appreciate hearing from those of you who have used one an >> what you think of it. >> >> Thanks >> Jo-Ann Bader >> Histology Facility Coordinator >> Molecular Oncology Group >> MUHC/RVH >> 687 Pine Ave. W - Rm. M11-53 >> Montreal, QC, H3A-1A1 >> Tel: 514-934-1934 Ext: 31780 >> Fax: 514-843-1479 >> email: jo-ann.bader@mcgill.ca >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> Confidentiality Notice ** The information contained in this message may >> be > privileged and is confidential information intended for the use of the > addressee listed above. If you are neither the intended recipient nor the > employee or agent responsible for delivering this message to the intended > recipient, you are hereby notified that any disclosure, copying, > distribution or the taking of any action in reliance on the contents of > this > information is strictly prohibited. If you have received this > communication > in error, please notify us immediately by replying to the message and > deleting it from your computer. Thank you. Saint Joseph's Health System, > Inc. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Fri May 4 10:05:13 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri May 4 10:05:18 2007 Subject: [Histonet] storing paraformaldehyde under inert gas In-Reply-To: <463A3B8E.6010801@umdnj.edu> References: <20070502160639.megui4a1gq4ok80g@webmail.stanford.edu> <463A3B8E.6010801@umdnj.edu> Message-ID: <6.0.0.22.1.20070504090236.01b54f98@gemini.msu.montana.edu> We store our Sigma paraformaldehyde inside a sealed plastic bag in the refrigerator (their 4C recommendation) but never under an inert gas. The bag is used to isolate the bottle, keep things clean more than anything. At 01:44 PM 5/3/2007, you wrote: > I have not heard of this and I just store my paraformaldehyde in its > original container, tightly closed. I suppose you could buy a glove box, > a closed chamber with gloves and inlet and outlet valves and use it to > close the paraform. jar with nitrogen in the chamber. Seems like a lot of > trouble ESPECIALLY since, if the lid is not 100% leak proof, you are > wasting your time and money. > >Geoff Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From rjbuesa <@t> yahoo.com Fri May 4 10:22:31 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri May 4 10:22:35 2007 Subject: [Histonet] Receiving all placentas In-Reply-To: Message-ID: <357087.91885.qm@web61214.mail.yahoo.com> We used to do what your OB/GYN dept. wants you to do. As to reimbursement you can charge the OB/GYN dept. (as we used to do) and they would then charge the patient's insurance. Ren? J. Kari Breal wrote: Our OB/GYN department is requesting we accession/receive all placentas. They want us to continue with the gross and micro for the one that meet the criteria for examination. For the normal placentas, they want us to gross the placenta, take a few sections and store these sections (uncut) for up to 20 years. Is there any pathology departments that receive all placentas? If you do, do you perform just a gross exam on the normal placentas or do you perform a gross and micro on all specimens? If you receive all placentas, do the insurance companies reimburse for the normal (the ones that do not meet the CAP guidelines for examination) placentas? Please contact me if your hospital does this or something similar with the placentas. Thank you, Kari Breal Histology Supervisor Alexian Brothers Medical Center 847-437-5500 ext. 5155 Fax 847-981-2023 brealk@alexian.net CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From derek.papalegis <@t> tufts.edu Fri May 4 10:35:12 2007 From: derek.papalegis <@t> tufts.edu (Derek Papalegis) Date: Fri May 4 10:35:20 2007 Subject: [Histonet] reprocessing Message-ID: <463B52B0.1060608@tufts.edu> I am reprocessing some tissues that did not process adequately and was curious as to if after they are followed backwards through xylene, 100, 95, 80 and 70, could the tissues go back into formalin with no adverse affects? Would it be better to simply leave them in 70% for the weekend. The one thing I don't want to do on a Friday afternoon is to sit around and stay late to process tissues. Thanks for your help. -- Derek Papalegis Histotechnician T-NEMC Animal Histology Core Division of Laboratory Animal Medicine Tufts University 136 Harrison Avenue Boston, MA 02111 phone: 617 636-2971 fax: 617 636-8354 From mcauliff <@t> umdnj.edu Fri May 4 10:42:05 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri May 4 10:42:52 2007 Subject: [Histonet] reprocessing In-Reply-To: <463B52B0.1060608@tufts.edu> References: <463B52B0.1060608@tufts.edu> Message-ID: <463B544D.50901@umdnj.edu> Hi Derek: While you could go all the way back to formalin I doubt if it would make a difference. I think storing in 70% over the weekend would be fine. Geoff Derek Papalegis wrote: > I am reprocessing some tissues that did not process adequately and was > curious as to if after they are followed backwards through xylene, > 100, 95, 80 and 70, could the tissues go back into formalin with no > adverse affects? Would it be better to simply leave them in 70% for > the weekend. The one thing I don't want to do on a Friday afternoon is > to sit around and stay late to process tissues. > > Thanks for your help. > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From rjbuesa <@t> yahoo.com Fri May 4 10:42:48 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri May 4 10:42:53 2007 Subject: [Histonet] reprocessing In-Reply-To: <463B52B0.1060608@tufts.edu> Message-ID: <437467.80000.qm@web61216.mail.yahoo.com> Returning to formalin will do nothing in any way. That tissue has already been fixed. As a matter of fact the most I used to do was going back to 100 EthOL and again to xylene and paraffin. Usually a tissue not well processed is already "ruined" will little hope for real improvement. The thing is doing the processing well from the initial step on the first time. Ren? J. Derek Papalegis wrote: I am reprocessing some tissues that did not process adequately and was curious as to if after they are followed backwards through xylene, 100, 95, 80 and 70, could the tissues go back into formalin with no adverse affects? Would it be better to simply leave them in 70% for the weekend. The one thing I don't want to do on a Friday afternoon is to sit around and stay late to process tissues. Thanks for your help. -- Derek Papalegis Histotechnician T-NEMC Animal Histology Core Division of Laboratory Animal Medicine Tufts University 136 Harrison Avenue Boston, MA 02111 phone: 617 636-2971 fax: 617 636-8354 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Bored stiff? Loosen up... Download and play hundreds of games for free on Yahoo! Games. From Terry.Marshall <@t> rothgen.nhs.uk Fri May 4 10:44:31 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri May 4 10:44:36 2007 Subject: [Histonet] Receiving all placentas Message-ID: <407F05A128805F4C879A33DBA32E618E20C2F3@TRFT-EX01.xRothGen.nhs.uk> Since by implication you do not do this now, I suggest you continue, rather than pursue this proposed insanity. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kari Breal Sent: 04 May 2007 15:51 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Receiving all placentas Our OB/GYN department is requesting we accession/receive all placentas. They want us to continue with the gross and micro for the one that meet the criteria for examination. For the normal placentas, they want us to gross the placenta, take a few sections and store these sections (uncut) for up to 20 years. Is there any pathology departments that receive all placentas? If you do, do you perform just a gross exam on the normal placentas or do you perform a gross and micro on all specimens? If you receive all placentas, do the insurance companies reimburse for the normal (the ones that do not meet the CAP guidelines for examination) placentas? Please contact me if your hospital does this or something similar with the placentas. Thank you, Kari Breal Histology Supervisor Alexian Brothers Medical Center 847-437-5500 ext. 5155 Fax 847-981-2023 brealk@alexian.net CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Mary.Vaughan <@t> RoswellPark.org Fri May 4 10:44:40 2007 From: Mary.Vaughan <@t> RoswellPark.org (Vaughan, Mary) Date: Fri May 4 10:44:42 2007 Subject: [Histonet] leptin receptor Message-ID: <6FF91AE4F1DC7743A6466E334EB865AE1A6E8F06@VERITY.roswellpark.org> Hi Everybody - I have a mo monoclonal antibody for Leptin receptor [not leptin] and am going to optimize it [human FFPE tissues]. Any advice? Best Regards, Mary Vaughan HT (ASCP) Research Specialist Roswell Park Cancer Institute Sci-601 Elm & Carlton Streets Buffalo, N Y 14263 phone: 716.845.3006 This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. From BrealK <@t> alexian.net Fri May 4 10:44:17 2007 From: BrealK <@t> alexian.net (Kari Breal) Date: Fri May 4 10:44:59 2007 Subject: [Histonet] CPT code 88172 for FNA cases Message-ID: How are other cytology departments charging for the cytotech performing the assessment for adequacy on FNA specimens? According to an article in the CAP today from Sep 06, 88172 can only be used if the pathologist renders the adequacy assessment. So does the cytotech not get to bill/charge for their time and technical skills for this task? For FNA's done by a cytotech or histotech, is 88173 (interpretation and report) the only charge? Thank you for your assistance! Kari Breal Histology Supervisor Alexian Brothers Medical Center 847-437-5500 ext. 5155 Fax 847-981-2023 brealk@alexian.net CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From kbradshaw <@t> lcpath.com Fri May 4 10:50:47 2007 From: kbradshaw <@t> lcpath.com (Kari Bradshaw) Date: Fri May 4 10:50:55 2007 Subject: [Histonet] CPT code 88172 for FNA cases In-Reply-To: Message-ID: That's correct. Kari Bradshaw Anatomic Pathology Manager Lower columbia Pathologists -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kari Breal Sent: Friday, May 04, 2007 8:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CPT code 88172 for FNA cases How are other cytology departments charging for the cytotech performing the assessment for adequacy on FNA specimens? According to an article in the CAP today from Sep 06, 88172 can only be used if the pathologist renders the adequacy assessment. So does the cytotech not get to bill/charge for their time and technical skills for this task? For FNA's done by a cytotech or histotech, is 88173 (interpretation and report) the only charge? Thank you for your assistance! Kari Breal Histology Supervisor Alexian Brothers Medical Center 847-437-5500 ext. 5155 Fax 847-981-2023 brealk@alexian.net CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Fri May 4 11:03:11 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri May 4 11:03:16 2007 Subject: [Histonet] reprocessing In-Reply-To: <463B52B0.1060608@tufts.edu> References: <463B52B0.1060608@tufts.edu> Message-ID: <6.0.0.22.1.20070504100117.01b3a5a8@gemini.msu.montana.edu> Go to Histonet Archives and look for Mickie Johnson's way of reprocessing tissues. I think he also published this in Histo Logic, Sakura Finetek website - it was a delightfully simple thing to do and you did not have to remove the paraffin to do it. Hopefully, he is looking in and will help with this again. At 09:35 AM 5/4/2007, you wrote: >I am reprocessing some tissues that did not process adequately and was >curious as to if after they are followed backwards through xylene, 100, >95, 80 and 70, could the tissues go back into formalin with no adverse >affects? Would it be better to simply leave them in 70% for the weekend. >The one thing I don't want to do on a Friday afternoon is to sit around >and stay late to process tissues. > >Thanks for your help. > >-- >Derek Papalegis >Histotechnician >T-NEMC Animal Histology Core >Division of Laboratory Animal Medicine >Tufts University 136 Harrison Avenue >Boston, MA 02111 >phone: 617 636-2971 >fax: 617 636-8354 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From TownsendD <@t> childrensdayton.org Fri May 4 11:12:43 2007 From: TownsendD <@t> childrensdayton.org (Dolores Townsend) Date: Fri May 4 11:13:01 2007 Subject: [Histonet] reprocessing Message-ID: Actually, you don't need to go to all that trouble with xylene and alcohol. We found out that all you have to do is melt down the block (if it was embedded), put the tissue on some absorbent paper and press gently to remove some of the paraffin, than just process again. The specimen can just go back directly into the processor in formalin. This has worked fine for us, and the pathologists can't see a difference. Dolores From gcallis <@t> montana.edu Fri May 4 11:17:47 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri May 4 11:17:53 2007 Subject: [Histonet] reprocessing paraffin blocks Message-ID: <6.0.0.22.1.20070504101611.01b0fe78@gemini.msu.montana.edu> Get this publication from Histo Logic on Sakura Finetek website. It was very simple and very little work overall. A Technique for Correcting Poorly Processed Paraffin Blocks. Michael L. Johnson, BS, HTL, HT(ASCP), Spokane, WA, May 2003;XXXVI(1):21. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From TJJ <@t> Stowers-Institute.org Fri May 4 11:29:47 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Fri May 4 11:30:06 2007 Subject: [Histonet] In vivo fluorescent antibody immunocytochemistry Message-ID: Has anybody successfully done experiments by injecting fluorescent-labeled antibodies into mouse organs in vivo? I'm currently searching the internet looking for information on how this is done, how long these antibodies would need to "incubate" prior to sacrifice, and whether it is possible to detect them in histological section afterwards. Thank you for any insight you can provide! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From jqb7 <@t> cdc.gov Fri May 4 11:43:11 2007 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Fri May 4 11:43:15 2007 Subject: [Histonet] reprocessing Message-ID: http://www.sakura-americas.com/histologic/pdf/03_may.pdf Page 21 From liz <@t> premierlab.com Fri May 4 11:50:17 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri May 4 11:50:27 2007 Subject: [Histonet] In vivo fluorescent antibody immunocytochemistry In-Reply-To: Message-ID: <000001c78e6c$4b46ece0$0d00a8c0@domain.Premier> Teri We have not looked at antibodies before, but we have looked at FITC and Cy3 tagged compounds. When we did this we looked at different time points up to 24 hours. You could use the liver and kidney as possible controls, once injected the antibody will be metabolized through the kidney and liver. We would snap freeze the tissue and then cut frozen sections. In our application we had some issues with fixation since our compound was water soluable. But we found that brief fixation in 10% NBF preserved the staining, but we also found that increased time in buffers or water, etc. would decrease staining. Personally I would start with a pilot of a few animals. The time points that you select will also depend it they are dosing iv verses ip or sc. Its going to take longer for ip and sc dosing to metabolize than the iv. I would try 1, 4, 8 and 24 hours to start with. After you cut the frozen don't mount them, just look at the section under the fluorescent microscope, you should see fluorescence in the kidney at all of those time points. You will get an idea of what type of staining you have and then you can fix and mount and be able to judge if you are losing staining when you fix, etc. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Teri Sent: Friday, May 04, 2007 10:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] In vivo fluorescent antibody immunocytochemistry Has anybody successfully done experiments by injecting fluorescent-labeled antibodies into mouse organs in vivo? I'm currently searching the internet looking for information on how this is done, how long these antibodies would need to "incubate" prior to sacrifice, and whether it is possible to detect them in histological section afterwards. Thank you for any insight you can provide! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1971 (20070110) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From JWEEMS <@t> sjha.org Fri May 4 11:57:09 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri May 4 11:57:36 2007 Subject: [Histonet] CPT code 88172 for FNA cases In-Reply-To: Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FF972B@sjhaexc02.sjha.org> That is our understanding. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Kari Breal Sent: Friday, May 04, 2007 11:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CPT code 88172 for FNA cases How are other cytology departments charging for the cytotech performing the assessment for adequacy on FNA specimens? According to an article in the CAP today from Sep 06, 88172 can only be used if the pathologist renders the adequacy assessment. So does the cytotech not get to bill/charge for their time and technical skills for this task? For FNA's done by a cytotech or histotech, is 88173 (interpretation and report) the only charge? Thank you for your assistance! Kari Breal Histology Supervisor Alexian Brothers Medical Center 847-437-5500 ext. 5155 Fax 847-981-2023 brealk@alexian.net CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From gcallis <@t> montana.edu Fri May 4 12:18:52 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri May 4 12:18:57 2007 Subject: QDot technology for Re: [Histonet] In vivo fluorescent antibody immunocytochemistry Message-ID: <6.0.0.22.1.20070504111648.01b3d758@gemini.msu.montana.edu> Check out QDot applications for this purpose. Invitrogen now owns the company and may have literature sources available, or maybe a visit to PUBMED or Google Scholar. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From lhadley <@t> iupui.edu Fri May 4 12:29:16 2007 From: lhadley <@t> iupui.edu (Baldridge, Lee Ann) Date: Fri May 4 12:29:20 2007 Subject: [Histonet] Unsubscribe Message-ID: <1E3324ECFB5D5F4DAFEDD17203EEBF21EC0BED@iu-mssg-mbx105.ads.iu.edu> Unsubscribe Please From dmccaig <@t> ckha.on.ca Fri May 4 13:00:28 2007 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Fri May 4 13:00:36 2007 Subject: [Histonet] cold ZN stain Message-ID: Has anyone used the cold method for ZN and have the recipe for the solutions or know of a vendor for premade solutions? How does it compare to the traditional method? Diana From tkngflght <@t> yahoo.com Fri May 4 13:06:08 2007 From: tkngflght <@t> yahoo.com (Cheryl Kerry) Date: Fri May 4 13:05:57 2007 Subject: [Histonet] Temp job openings and a whole bunch of permanent positions... In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FF972B@sjhaexc02.sjha.org> References: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FF972B@sjhaexc02.sjha.org> Message-ID: <005501c78e76$e478b6f0$6501a8c0@FSDESKTOP> Hello everyone! We hope you had a great lab week! TEMPS: As always, we're looking for a couple of great histology travelers. We have openings in a number of labs--a couple I've benched in myself so I can vouch that you'll have a great assignment! If you're curious but have never temped before, I'll spend all the time you need to answer your questions and to be certain you & your family are comfortable before you ever leave your front door. Call the 800 any time before 10PM and I'll pick up (or call you right back--please leave a message!!). I'd like to get a good fit for these as they are good places to work and really need the help due to growth. We have five current openings and if we don't have what you want now, registration takes a few minutes and we'll work to find it for you. PERMANENTS: We also have a number of new permanent positions, including routine bench, grossing and management. (Too many to list here.) I'm careful with the labs we work with--if I wouldn't work there, I would never expect you to, either! Give us a call-five minutes could mean being HAPPY to go to work each day with a paycheck to reflect our efforts.we all deserve at LEAST that. Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. 281.852.9457 800.756.3309 Sign up for the FREE newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. From p.jung <@t> freesurf.ch Fri May 4 13:26:53 2007 From: p.jung <@t> freesurf.ch (p.jung@freesurf.ch) Date: Fri May 4 13:27:00 2007 Subject: [Histonet] (no subject) Message-ID: <45C1FBD90004D956@mta-fs-be-02.sunrise.ch> Medizinische Genetik Universit?tsklinikum T?bingen Patrick Jung Calwerstra?e 7 72076 T?bingen 07071-29-72306 Hi all, I want to embed rat brain ( halves) in paraffin with the Leica TP 1020 ( Ethanol/Xylene). Unfortunately the rat brain were useless with the program installed for mice brains....Could you give me a program for the Leica to fix the rat brain ? Or have you any idea on the fixation times you should respect for rat brains? Thanks to you all. Best regards paddy Neu: Das erste ADSL-Abo ohne Monatsgeb?hr! Steigen Sie jetzt auf sunrise ADSL free um. http://www.sunrise.ch/privatkunden/iminternetsurfen/adsl/adsl_abosundpreise/adsl_gelegenheitssurfer/adsl_free.htm From pvalente <@t> sbcglobal.net Fri May 4 16:12:06 2007 From: pvalente <@t> sbcglobal.net (Patricia Valente) Date: Fri May 4 16:12:10 2007 Subject: [Histonet] 2 open HT positions Message-ID: <164414.73949.qm@web81707.mail.mud.yahoo.com> Please join our Urology lab team in beautiful sunny San Antonio. We have two FT Histotech positions. Interesting work in a friendly place for an experienced self starter. please contact - pvalente@uropathllc.com or bbailey@uropathllc.com From Rcartun <@t> harthosp.org Sat May 5 06:48:58 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Sat May 5 06:49:12 2007 Subject: [Histonet] Receiving all placentas In-Reply-To: References: Message-ID: <463C36EA0200007700005A5D@gwmail.harthosp.org> This request is ridiculous. Do you know why they are requesting this? Legal reasons? Research? Insurance companies will not pay for the handling of normal placentas from uncomplicated pregnancies. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Kari Breal" 05/04/07 10:50 AM >>> Our OB/GYN department is requesting we accession/receive all placentas. They want us to continue with the gross and micro for the one that meet the criteria for examination. For the normal placentas, they want us to gross the placenta, take a few sections and store these sections (uncut) for up to 20 years. Is there any pathology departments that receive all placentas? If you do, do you perform just a gross exam on the normal placentas or do you perform a gross and micro on all specimens? If you receive all placentas, do the insurance companies reimburse for the normal (the ones that do not meet the CAP guidelines for examination) placentas? Please contact me if your hospital does this or something similar with the placentas. Thank you, Kari Breal Histology Supervisor Alexian Brothers Medical Center 847-437-5500 ext. 5155 Fax 847-981-2023 brealk@alexian.net CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From rsrichmond <@t> aol.com Sun May 6 04:06:51 2007 From: rsrichmond <@t> aol.com (rsrichmond@aol.com) Date: Sun May 6 04:07:07 2007 Subject: [Histonet] Re: Receiving all placentas In-Reply-To: <200705051301.a39463cb85d71@rly-mg10.mx.aol.com> References: <200705051301.a39463cb85d71@rly-mg10.mx.aol.com> Message-ID: <8C95DD504F8EB5E-F74-C881@mblk-d37.sysops.aol.com> Kari Breal, the Histology Supervisor Alexian Brothers Medical Center at 847-437-5500 ext. 5155 (I'm going to assume this is in the USA - my Internet access is limited today) asks: >>Our OB/GYN department is requesting we accession/receive all placentas. They want us to continue with the gross and micro for the one that meet the criteria for examination. For the normal placentas, they want us to gross the placenta, take a few sections and store these sections (uncut) for up to 20 years.<< Did your pathologists have no input into this decision? If they're allowed an opinion, I'd suggest they work with your hospital's perinatal committee on this issue. The gross-only examination (CPT code 88300) pays very much less than the microscopic (88307 for placentas). Whether insurance companies pay either of these - and how much they pay - varies from state to state - remember that in most states 80% of deliveries are on Medicaid or its local equivalent. By "store these sections uncut" I suppose you mean store the uncut paraffin blocks. Since in most places paraffin blocks are retained for at most ten years, this policy would require separate storage of placenta blocks. (Actually 20 years is a little short - as much as 25 years would probably be required - if your kid flunks out of college, sue the obstetrician that delivered him.) I'm seeing increasing numbers of hospitals where all placentas get microscopic examinations, and apparently the third parties are paying. Placentas are submitted for examination entirely without history, with hissy-fits following the pathologist's attempts to get any, so that the quality of the examinations is quite low. If the OB-GYN's want a gross examination only, they should do the examination themselves. My advice - which I've never seen followed - would be to have all placentas submitted in adequate quantities of formalin, with the pathology department holding them for a week. If the OB-GYN wants a placental examination, they can order it, with a special history sheet included. If they don't order an examination, the charts are reviewed by somebody in the Risk Management department when mother and baby are discharged, and examination is ordered by the Risk Management department if guidelines indicate it's needed. (Remember that some indications for examination are not apparent at the time of the delivery.) Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From pruegg <@t> ihctech.net Sun May 6 17:20:58 2007 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Sun May 6 17:19:56 2007 Subject: [Histonet] Receiving all placentas In-Reply-To: <463C36EA0200007700005A5D@gwmail.harthosp.org> Message-ID: <200705062219.l46MJglP083729@pro12.abac.com> It sounds like your OB department is trying to get you to do their research for them for free. I went to a talk once by a Pathologist who was suggesting that we look at all placentas, he thinks we are missing a lot of pathology from not examining the placentas more carefully in anatomic pathology, but he had no plan for who would pay for this. I see it as a research interest project, your OB department should apply for a research grant to fund anatomic path examination and storage of sections on all placentas. My two cents worth. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Saturday, May 05, 2007 5:49 AM To: Kari Breal; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Receiving all placentas This request is ridiculous. Do you know why they are requesting this? Legal reasons? Research? Insurance companies will not pay for the handling of normal placentas from uncomplicated pregnancies. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Kari Breal" 05/04/07 10:50 AM >>> Our OB/GYN department is requesting we accession/receive all placentas. They want us to continue with the gross and micro for the one that meet the criteria for examination. For the normal placentas, they want us to gross the placenta, take a few sections and store these sections (uncut) for up to 20 years. Is there any pathology departments that receive all placentas? If you do, do you perform just a gross exam on the normal placentas or do you perform a gross and micro on all specimens? If you receive all placentas, do the insurance companies reimburse for the normal (the ones that do not meet the CAP guidelines for examination) placentas? Please contact me if your hospital does this or something similar with the placentas. Thank you, Kari Breal Histology Supervisor Alexian Brothers Medical Center 847-437-5500 ext. 5155 Fax 847-981-2023 brealk@alexian.net CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Sun May 6 19:11:43 2007 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun May 6 19:11:55 2007 Subject: [Histonet] reprocessing Message-ID: I agree Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dolores Townsend Sent: Saturday, 5 May 2007 2:13 AM To: histonet@lists.utsouthwestern.edu; Derek Papalegis Subject: Re: [Histonet] reprocessing Actually, you don't need to go to all that trouble with xylene and alcohol. We found out that all you have to do is melt down the block (if it was embedded), put the tissue on some absorbent paper and press gently to remove some of the paraffin, than just process again. The specimen can just go back directly into the processor in formalin. This has worked fine for us, and the pathologists can't see a difference. Dolores _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From scorpionrider <@t> cox.net Sun May 6 20:37:47 2007 From: scorpionrider <@t> cox.net (Mark Turner) Date: Sun May 6 20:37:53 2007 Subject: [Histonet] reprocessing In-Reply-To: Message-ID: <000001c79048$50deeb20$6400a8c0@D8P565C1> We do the same thing when we need to reprocess tissue. Saves quite a bit of time and is less harsh on the tissue, particularly for IHC procedures. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Sunday, May 06, 2007 5:12 PM To: Dolores Townsend; histonet@lists.utsouthwestern.edu; Derek Papalegis Subject: RE: [Histonet] reprocessing I agree Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dolores Townsend Sent: Saturday, 5 May 2007 2:13 AM To: histonet@lists.utsouthwestern.edu; Derek Papalegis Subject: Re: [Histonet] reprocessing Actually, you don't need to go to all that trouble with xylene and alcohol. We found out that all you have to do is melt down the block (if it was embedded), put the tissue on some absorbent paper and press gently to remove some of the paraffin, than just process again. The specimen can just go back directly into the processor in formalin. This has worked fine for us, and the pathologists can't see a difference. Dolores _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From djemge <@t> aol.com Mon May 7 11:28:30 2007 From: djemge <@t> aol.com (djemge@aol.com) Date: Mon May 7 11:28:50 2007 Subject: [Histonet] Help With In Situ Message-ID: <8C95EDBE21D2102-EA0-8CC3@WEBMAIL-RC15.sysops.aol.com> One of the Grad Students asked me to post her question. The Mouse Testes and Mouse Brain tissue for ISH Sox3 was kept at 4 degrees, 4% PFA fixed overnight, 30% sucrose cryoprotected until it sank, embedded in OCT, flash frozen. The ISH on the Mouse Brain was very good, but not the Mouse Testes. Two photos ISH Sox 3 Testes and ISH Sox 3 Brain are in the image gallery at histonet.org. Thanks, Donna Here's Monica's question: I want to know if anyone else had experience with ISH on adult mouse testis cross-sections and if they've seen this background staining that specifically binds to elongated spermatid heads... And of course if anyone has been able to block against this. My sox3 probe is supposed to bind to the undifferentiated spermatogonia along the basement membrane of the seminiferous tubules. This staining appeared before the positive staining (approximately 3-4h after incubation) among the spermatid heads towards the lumen of the tubules. I have been using 4%PFA fixed testes sections and blocking with 10% lamb serum. This staining has appeared with both an 820b probe and 440b probe. -- Monica M Laronda Graduate Student, Jameson Lab Northwestern University Feinberg School of Medicine Chicago, IL m-laronda@northwestern.edu Phone: 312-503-2036 Donna J Emge, HT(ASCP) Northwestern University Feinberg School of Medicine Chicago, IL djemge@aol.com d-emge@northwestern.edu Phone: 312-503-2036 ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From Melissa.Gonzalez <@t> cellgenesys.com Mon May 7 12:35:52 2007 From: Melissa.Gonzalez <@t> cellgenesys.com (Melissa Gonzalez) Date: Mon May 7 12:36:14 2007 Subject: [Histonet] Regulatory Tcells (FoxP3) IHC In-Reply-To: <20070506170017.0D54227F0BE@mail.cellgenesys.com> References: <20070506170017.0D54227F0BE@mail.cellgenesys.com> Message-ID: Hi all, Anyone have any experience with this? How well does the Abcam antibody work on FFPE human tissues? What's a good positive control? Thanks Melissa From pruegg <@t> ihctech.net Mon May 7 13:57:33 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon May 7 13:57:40 2007 Subject: [Histonet] Regulatory Tcells (FoxP3) IHC In-Reply-To: Message-ID: <006c01c790d9$92dbcd90$6501a8c0@Patsy> Thymus should be a good control for T cells. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Melissa Gonzalez Sent: Monday, May 07, 2007 11:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Regulatory Tcells (FoxP3) IHC Hi all, Anyone have any experience with this? How well does the Abcam antibody work on FFPE human tissues? What's a good positive control? Thanks Melissa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Mon May 7 14:13:26 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon May 7 14:13:40 2007 Subject: [Histonet] Regulatory Tcells (FoxP3) IHC In-Reply-To: <006c01c790d9$92dbcd90$6501a8c0@Patsy> References: <006c01c790d9$92dbcd90$6501a8c0@Patsy> Message-ID: <6.0.0.22.1.20070507131119.01b1d8c8@gemini.msu.montana.edu> The technical data sheet should provide control information. Spleen should work with this according to the eBiosciences data sheet/technical information. At 12:57 PM 5/7/2007, you wrote: >Thymus should be a good control for T cells. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Melissa >Gonzalez >Sent: Monday, May 07, 2007 11:36 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Regulatory Tcells (FoxP3) IHC > >Hi all, >Anyone have any experience with this? How well does the Abcam antibody >work on FFPE human tissues? What's a good positive control? >Thanks >Melissa Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 From gcallis <@t> montana.edu Mon May 7 14:23:08 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon May 7 14:23:11 2007 Subject: [Histonet] FOX P3 antibody Message-ID: <6.0.0.22.1.20070507132141.01b13268@gemini.msu.montana.edu> The following was copied from eBioscience website. The photographs they showed were murine spleen/frozen section and also human tonsil. The Foxp3 antibodies have been validated for intracellular staining with flow cytometric analysis. The protocols and staining buffers have been developed for optimal and consistent staining Foxp3 staining while preserving surface antigen staining. Furthermore, the protocol was designed for robustness, reproducibility, ease-of-use, as well as flexibility, to allow researchers to stain at their convenience. In addition, the antibodies have been reported useful for immunohistological staining (both frozen and paraffin sections) and western blotting of endogenous protein from total human PBMCs or mouse splenocytes, without the need for enrichment of Tregs. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From tp2 <@t> medicine.wisc.edu Mon May 7 14:28:47 2007 From: tp2 <@t> medicine.wisc.edu (Thomas Pier) Date: Mon May 7 14:29:23 2007 Subject: [Histonet] Regulatory Tcells (FoxP3) IHC Message-ID: <463F37A0020000DF000070AF@gwmail.medicine.wisc.edu> I've used the Abcam FOXP3 antibody in the past and had great results with it. I pretty much just followed the data sheet that came with the antibody. Good positive controls would be tonsil or lymphnode. Tom Pier >>> Gayle Callis 05/07/07 2:13 PM >>> The technical data sheet should provide control information. Spleen should work with this according to the eBiosciences data sheet/technical information. At 12:57 PM 5/7/2007, you wrote: >Thymus should be a good control for T cells. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Melissa >Gonzalez >Sent: Monday, May 07, 2007 11:36 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Regulatory Tcells (FoxP3) IHC > >Hi all, >Anyone have any experience with this? How well does the Abcam antibody >work on FFPE human tissues? What's a good positive control? >Thanks >Melissa Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mauger <@t> email.chop.edu Mon May 7 14:42:07 2007 From: mauger <@t> email.chop.edu (Joanne Mauger) Date: Mon May 7 14:42:50 2007 Subject: [Histonet] Regulatory Tcells (FoxP3) IHC Message-ID: Hi Melissa, We use the Abcam antibody #ab2481, goat polyclonal at 1:400 with EDTA pH8 retrieval.It works well on FFPE, we use tonsil for control. The % of positive cells will vary with different tonsils. We stain human tissue, and I believe I have stained mouse tissue with this Ab also. Jo Mauger From alaskagirl1950 <@t> yahoo.com Mon May 7 14:44:47 2007 From: alaskagirl1950 <@t> yahoo.com (Patricia Adams) Date: Mon May 7 14:44:51 2007 Subject: [Histonet] IHC antibodies for animals Message-ID: <511336.93307.qm@web52501.mail.re2.yahoo.com> Hello all! Now that I am working with animals, I am looking for antibodies that react on all species. But right now canine is the most important. I used a CD20 and a CD43, human tonsil as the control. Of course my controls were beautiful, but the canine pt. did not work and it should have. CD20 and CD43 were all the choices I had to pick for B-cell and T-cell. Could you please give me some advice? We have a very small budget and I do not have the choice of buying antibodies just to see if they will work on animals. Thank you very much, Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From ploykasek <@t> phenopath.com Mon May 7 14:45:09 2007 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Mon May 7 14:45:19 2007 Subject: FW: [Histonet] Receiving all placentas In-Reply-To: Message-ID: Dear Kari, I have worked at institutions in the past where this was the practice. I am not sure about the reimbursement issues. It is definitely a practice due to medical/ legal concerns. The majority of ' routine' placentas, a P.A. Did the gross exam, submitted material to be processed & embedded & the blocks were filed. These gross exams were usually done 1-2 X's a week, not every day, and the blocks were processed once a week. No H&E was cut unless requested, usually initiated by the ob/gyn. Placentas with any abnormal history or gross finding were submitted for routine micro. I don't remember the other details. I know the ob/gyn & neonat docs really liked this process. Saved them countless problems on numerous occasions. Hope this helps. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > Our OB/GYN department is requesting we accession/receive all placentas. > They want us to continue with the gross and micro for the one that meet > the criteria for examination. For the normal placentas, they want us to > gross the placenta, take a few sections and store these sections (uncut) > for up to 20 years. > > > Is there any pathology departments that receive all placentas? If you > do, do you perform just a gross exam on the normal placentas or do you > perform a gross and micro on all specimens? > > If you receive all placentas, do the insurance companies reimburse for > the normal (the ones that do not meet the CAP guidelines for > examination) placentas? > > Please contact me if your hospital does this or something similar with > the placentas. > > Thank you, > > > > Kari Breal > Histology Supervisor > Alexian Brothers Medical Center > 847-437-5500 ext. 5155 > Fax 847-981-2023 > brealk@alexian.net > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for > the sole use of the intended recipient(s) and may contain confidential and > privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------ End of Forwarded Message ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From SDrew <@t> uwhealth.org Mon May 7 15:03:28 2007 From: SDrew <@t> uwhealth.org (Drew Sally A.) Date: Mon May 7 15:03:35 2007 Subject: [Histonet] Leishmania Message-ID: Could someone direct us to a place/person who might run an antibody against Leishmania? We have a pathologist asking questions about it, and all our usual source don't list it. Thank you! Sally Ann Drew, MT(ASCP) IHC/ISH Laboratory University of Wisconsin Hosp. & Clinics Madison, WI 53792 (608)265-6596 From pruegg <@t> ihctech.net Mon May 7 15:16:55 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon May 7 15:17:02 2007 Subject: [Histonet] IHC antibodies for animals In-Reply-To: <511336.93307.qm@web52501.mail.re2.yahoo.com> Message-ID: <007001c790e4$ac01c990$6501a8c0@Patsy> Patricia, I have used CD20 from Lab Vision on dogs, this does not require any pretreatment AR. Also used cd79a from Dako for B cells on dog tissue. For T cells I highly recommend Lab Vision's rab monoclonal CD3, it works great with citrate HIER on dogs. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patricia Adams Sent: Monday, May 07, 2007 1:45 PM To: HistoNet Subject: [Histonet] IHC antibodies for animals Hello all! Now that I am working with animals, I am looking for antibodies that react on all species. But right now canine is the most important. I used a CD20 and a CD43, human tonsil as the control. Of course my controls were beautiful, but the canine pt. did not work and it should have. CD20 and CD43 were all the choices I had to pick for B-cell and T-cell. Could you please give me some advice? We have a very small budget and I do not have the choice of buying antibodies just to see if they will work on animals. Thank you very much, Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From portera <@t> msu.edu Mon May 7 15:26:43 2007 From: portera <@t> msu.edu (Amy Porter) Date: Mon May 7 15:26:56 2007 Subject: [Histonet] IHC antibodies for animals References: <511336.93307.qm@web52501.mail.re2.yahoo.com> Message-ID: <006301c790e6$08e2af20$8e7a0923@histolab> Patricia - You could try Dako Rabbit Polyclonal CD3 for T-Cells if you are just looking to identify TCells. You might try going to VMRD.com and looking for canine antibodies there. We use Dako CD3 here for numerous species and it works well. Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 355-6475 ext 1480 Fax: (517) 432-1368 Email: portera@msu.edu Web: www.humanpathology.msu.edu ----- Original Message ----- From: "Patricia Adams" To: "HistoNet" Sent: Monday, May 07, 2007 3:44 PM Subject: [Histonet] IHC antibodies for animals > Hello all! > Now that I am working with animals, I am looking > for antibodies that react on all species. But > right now canine is the most important. > I used a CD20 and a CD43, human tonsil as the > control. Of course my controls were beautiful, > but the canine pt. did not work and it should > have. > CD20 and CD43 were all the choices I had to pick > for B-cell and T-cell. > Could you please give me some advice? We have a > very small budget and I do not have the choice of > buying antibodies just to see if they will work > on animals. > Thank you very much, > > Patricia Adams > ----- > Fight back spam! Download the Blue Frog. > http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From shive003 <@t> umn.edu Mon May 7 15:56:49 2007 From: shive003 <@t> umn.edu (Jan Shivers) Date: Mon May 7 15:56:53 2007 Subject: [Histonet] IHC antibodies for animals References: <511336.93307.qm@web52501.mail.re2.yahoo.com> Message-ID: <005b01c790ea$3b4b5c10$a1065486@auxs.umn.edu> Patricia, Are you looking for only CD markers that work on non-human mammalian species, or any antibodies in general? My CD sources: Rabbit polyclonal CD3 (LabVision/NeoMarkers; RB-9039-P; works well on everything.) Mouse monoclonal CD79a (LabVision/NeoMarkers; MS-357-P; works moderately well, but some nonspecific staining) Mouse monoclonal CD18 canine (Leukocyte Antigen Biology Lab/UCDavis; CA16.3C10; works well on canines only) Mouse monoclonal CD18 feline (Leukocyte Antigen Biology Lab/UCDavis; FE3.9F2; works well on felines only) Mouse monoclonal CD45RA canine (Leukocyte Antigen Biology Lab/UCDavis; CA21.4B3; works well on canines only) Leukocyte Antigen Biology Lab also carries other CD markers, but they can only be used on frozen tissues; same goes for VMRD/Pullman, WA. If you want information on tissue/tumor marker or immunoglobulin reactivity in non-human tissues, you can write me directly for the info. I use about 75 of them in my lab. Jan Shivers IHC/Histo/EM Section Head Univ. of Minnesota Veterinary Diagnostic Lab 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu ----- Original Message ----- From: "Patricia Adams" To: "HistoNet" Sent: Monday, May 07, 2007 2:44 PM Subject: [Histonet] IHC antibodies for animals > Hello all! > Now that I am working with animals, I am looking > for antibodies that react on all species. But > right now canine is the most important. > I used a CD20 and a CD43, human tonsil as the > control. Of course my controls were beautiful, > but the canine pt. did not work and it should > have. > CD20 and CD43 were all the choices I had to pick > for B-cell and T-cell. > Could you please give me some advice? We have a > very small budget and I do not have the choice of > buying antibodies just to see if they will work > on animals. > Thank you very much, > > Patricia Adams > ----- > Fight back spam! Download the Blue Frog. > http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From algranth <@t> u.arizona.edu Mon May 7 15:58:16 2007 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Mon May 7 15:59:21 2007 Subject: [Histonet] recycled xylene question Message-ID: <4.3.2.7.2.20070507135321.00def1e0@algranth.inbox.email.arizona.edu> I'm asking this question for a friend and I'm including her email address so you can respond directly to her. Her lab has been having problems with water in their recycled xylene. She is wondering if anybody else has had this problem and what they did to prevent it. They have a CBG recycler and process on a VIP 5 tissue processor. Send responses to: MAWeiss@scal.kp.org Thanks and Happy Monday! Andi ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From rjbuesa <@t> yahoo.com Mon May 7 16:06:36 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon May 7 16:06:40 2007 Subject: [Histonet] recycled xylene question In-Reply-To: <4.3.2.7.2.20070507135321.00def1e0@algranth.inbox.email.arizona.edu> Message-ID: <740423.99073.qm@web61213.mail.yahoo.com> She should check the diverting valve, the one that diverts the "waste" to the waste container and that shuts down when the temperature reaches the BP of xylene. If the valve is not working correctly some "waste" will be diverted to the "recycled xylene" container and will contaminate it. Ren? J. Andrea Grantham wrote: I'm asking this question for a friend and I'm including her email address so you can respond directly to her. Her lab has been having problems with water in their recycled xylene. She is wondering if anybody else has had this problem and what they did to prevent it. They have a CBG recycler and process on a VIP 5 tissue processor. Send responses to: MAWeiss@scal.kp.org Thanks and Happy Monday! Andi ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From pruegg <@t> ihctech.net Mon May 7 16:26:25 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon May 7 16:26:34 2007 Subject: [Histonet] IHC antibodies for animals In-Reply-To: <006101c790ea$d2fc0ff0$a1065486@auxs.umn.edu> Message-ID: <008101c790ee$63267770$6501a8c0@Patsy> Jan, I used the Lab Vision polyclonal CD20. Patsy -----Original Message----- From: Jan Shivers [mailto:shive003@umn.edu] Sent: Monday, May 07, 2007 3:01 PM To: Patsy Ruegg Subject: Re: [Histonet] IHC antibodies for animals Hi Patsy, Could you tell me which CD20 from Lab Vision you use? I see they have one polyclonal and three monoclonal CD20s. I'm in a search for another B cell marker, since Dako discontinued selling B Lymphocyte Antigen 36. Thanks so much for any help you can provide. Jan Shivers U of MN Vet Diag Lab shive003@umn.edu ----- Original Message ----- From: "Patsy Ruegg" To: "'Patricia Adams'" ; "'HistoNet'" Sent: Monday, May 07, 2007 3:16 PM Subject: RE: [Histonet] IHC antibodies for animals > Patricia, > I have used CD20 from Lab Vision on dogs, this does not require any > pretreatment AR. Also used cd79a from Dako for B cells on dog tissue. > For > T cells I highly recommend Lab Vision's rab monoclonal CD3, it works great > with citrate HIER on dogs. > Patsy > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patricia > Adams > Sent: Monday, May 07, 2007 1:45 PM > To: HistoNet > Subject: [Histonet] IHC antibodies for animals > > Hello all! > Now that I am working with animals, I am looking > for antibodies that react on all species. But > right now canine is the most important. > I used a CD20 and a CD43, human tonsil as the > control. Of course my controls were beautiful, > but the canine pt. did not work and it should > have. > CD20 and CD43 were all the choices I had to pick > for B-cell and T-cell. > Could you please give me some advice? We have a > very small budget and I do not have the choice of > buying antibodies just to see if they will work > on animals. > Thank you very much, > > Patricia Adams > ----- > Fight back spam! Download the Blue Frog. > http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tkngflght <@t> yahoo.com Mon May 7 19:23:21 2007 From: tkngflght <@t> yahoo.com (Cheryl Kerry) Date: Mon May 7 20:23:42 2007 Subject: [Histonet] Staining during processing In-Reply-To: <008101c790ee$63267770$6501a8c0@Patsy> References: <006101c790ea$d2fc0ff0$a1065486@auxs.umn.edu> <008101c790ee$63267770$6501a8c0@Patsy> Message-ID: <001401c79107$15f95990$6501a8c0@FSDESKTOP> Hi All! A client is trying to stain biopsies on processing to aid in orientation. They've tried eosin in the processor alcohols and wanted to know if there are any other ways that allow the embedders to see tissue detail but that won't interfere with subsequent staining (or at least minimal interference.) Any suggestions welcome and I'll pass them along-- Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc 800.756.3309 Currently seeking temporary travel histologist!! From vanmetmj <@t> pbrc.edu Mon May 7 23:32:44 2007 From: vanmetmj <@t> pbrc.edu (Montina Van Meter) Date: Mon May 7 23:33:18 2007 Subject: [Histonet] Louisiana State Meeting Info Message-ID: <463FB71C0200005000003584@PBRCFS07.pbrc.edu> Hello, The Louisiana Society for Histotechnology is pleased to announce the 24th Annual Symposium/Convention "Cajun Style", will be held June 1 & 2, 2007. The meeting will be held in Lafayette, LA at the Best Western Hotel Acadiana, 1801 W. Pinhook Road. For reservations call 337-233-8120 or 1-800-887-7371 and mention you are with the LSH group. Do to relocation of many of our members we would ask everyone who would like to receive a brochure/membership form in the mail or by fax, to please contact me via email or phone at vanmetmj@pbrc.edu or 225-603-0953. Walk-ins are always welcome!!! The LSH would also like to extend the invitation to our fellow techs from Mississippi, Arkansas, Alabama and Texas, to please consider attending our meeting, you are always welcome. We would encourage everyone to attend in order to build our networking potential, earn those valuable CEU's and enjoy some warm Cajun hospitality. We have a variety of topics that will be presented by experienced speakers that promise to benefit everyone. The attendees will also have access to several well known scientific vendor exhibits during the entire symposium. I have listed below the schedule and workshops and encourage you to come on over to Lafayette! Fri., June 1, 2007, 8-11:30 AM: WS#1 Ready or Not Here It Comes: Microwave Technology WS#2 Histology Nuts and Bolts - Back to Basics Fri., June 1, 2007, 1-4:30 PM: WS#3 Is Your Tissue Processor Fighting You? Fight Back. WS#4 Introduction to Immunos Sat., June 2, 2007, 8-11:30 AM: WS#5 What Every Histotech Should Know About Water WS#6 Which Plastic Should Be Used To Embed This Project? Sat., June2, 2007, 1-4:30 PM: WS#7 What Code Should I Use? WS#8 Latest Advances in Immunohistochemistry CONFIDENTIALITY NOTICE Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this E-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately. From Kemlo.Rogerson <@t> waht.swest.nhs.uk Tue May 8 01:21:44 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue May 8 01:21:52 2007 Subject: [Histonet] Staining during processing Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0124768B@wahtntex2.waht.swest.nhs.uk> Dip the biopsies in alcoholic eosin BEFORE processing. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Wherever there is a human being, there is an opportunity for kindness. --Seneca This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From rjbuesa <@t> yahoo.com Tue May 8 07:30:52 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue May 8 07:30:58 2007 Subject: [Histonet] Staining during processing In-Reply-To: <001401c79107$15f95990$6501a8c0@FSDESKTOP> Message-ID: <84741.1408.qm@web61219.mail.yahoo.com> Cheryl: There are 2 different issues: if the biopsy is very small, staining it will prevent losing it, but that has nothing to do with orientation; what orientation has to have such a small biopsy? If the biopsy is a very small skin punch any histotech can orient it, and if they are needle biospies of any sort, they do not need orientation at all. So if not losing it is the issue, then the best option is to stain them (either before or during processing) but if your client does not like to have the Bx partially stained then tell your client to be careful, to wrap the Bx in paper or between sponges and just be careful. It is evident that the HT you sent to your client did not know what to do! Ren? J. Cheryl Kerry wrote: Hi All! A client is trying to stain biopsies on processing to aid in orientation. They've tried eosin in the processor alcohols and wanted to know if there are any other ways that allow the embedders to see tissue detail but that won't interfere with subsequent staining (or at least minimal interference.) Any suggestions welcome and I'll pass them along-- Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc 800.756.3309 Currently seeking temporary travel histologist!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From rjbuesa <@t> yahoo.com Tue May 8 07:30:52 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue May 8 07:31:00 2007 Subject: [Histonet] Staining during processing In-Reply-To: <001401c79107$15f95990$6501a8c0@FSDESKTOP> Message-ID: <84741.1408.qm@web61219.mail.yahoo.com> Cheryl: There are 2 different issues: if the biopsy is very small, staining it will prevent losing it, but that has nothing to do with orientation; what orientation has to have such a small biopsy? If the biopsy is a very small skin punch any histotech can orient it, and if they are needle biospies of any sort, they do not need orientation at all. So if not losing it is the issue, then the best option is to stain them (either before or during processing) but if your client does not like to have the Bx partially stained then tell your client to be careful, to wrap the Bx in paper or between sponges and just be careful. It is evident that the HT you sent to your client did not know what to do! Ren? J. Cheryl Kerry wrote: Hi All! A client is trying to stain biopsies on processing to aid in orientation. They've tried eosin in the processor alcohols and wanted to know if there are any other ways that allow the embedders to see tissue detail but that won't interfere with subsequent staining (or at least minimal interference.) Any suggestions welcome and I'll pass them along-- Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc 800.756.3309 Currently seeking temporary travel histologist!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From Marirose.Satterfield <@t> MercyMemorial.org Tue May 8 10:03:41 2007 From: Marirose.Satterfield <@t> MercyMemorial.org (Satterfield, Marirose) Date: Tue May 8 10:03:53 2007 Subject: [Histonet] Histology Lab ventilation Message-ID: I was wondering if any one could point me in the right direction. We are having ventilation problems in our lab. The lab director wants to know if there are any standards for air flow in the Histology Lab or how much air should be circulated in any given time. The only standards I know about are for fumes. I thought we should just ask a company who specializes in ventilation but the Lab Director seems to think they may not be familiar with a Histology Lab. Any thoughts? Marirose Satterfield Histology Supervisor Mercy Memorial Hospital From rjbuesa <@t> yahoo.com Tue May 8 10:18:34 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue May 8 10:18:37 2007 Subject: [Histonet] Histology Lab ventilation In-Reply-To: Message-ID: <860228.55716.qm@web61219.mail.yahoo.com> Our standard was four to five complete air exchanges per hour. That should not be taken as a rigid standard and could vary depending on the size (air volume) of the room or how many chemicals are used. Also if in the room there is a fumes hood. The air exchange rate has to be also combined with a monitoring program for chemical exposure, specially for formalin and xylene, for those labs still using one or both of those dangerous chemicals. Hope this will help you! Ren? J. "Satterfield, Marirose" wrote: I was wondering if any one could point me in the right direction. We are having ventilation problems in our lab. The lab director wants to know if there are any standards for air flow in the Histology Lab or how much air should be circulated in any given time. The only standards I know about are for fumes. I thought we should just ask a company who specializes in ventilation but the Lab Director seems to think they may not be familiar with a Histology Lab. Any thoughts? Marirose Satterfield Histology Supervisor Mercy Memorial Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From sjchtascp <@t> yahoo.com Tue May 8 10:39:46 2007 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Tue May 8 10:39:49 2007 Subject: [Histonet] Histology position wanted Message-ID: <20070508153946.72057.qmail@web38203.mail.mud.yahoo.com> I'm seeking an part time or as needed histology position in the So.Wisconsin or No.Illinois area. Thanks you, Steven Coakley Beloit, WI --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From dpahisto <@t> yahoo.com Tue May 8 12:27:35 2007 From: dpahisto <@t> yahoo.com (Cindy DuBois) Date: Tue May 8 12:27:41 2007 Subject: [Histonet] Placentas Message-ID: <769673.20813.qm@web33409.mail.mud.yahoo.com> We do this for one of our hospitals. We do charge the patient / insurance for a "Gross Only" on the placentas we accession as a gross only specimen. We were told that since the child and / or parents can sue the hospital up to 20 years later for any perceived problems that may have arisen out of the pregnancy or labor, this was to cover the hospital legally. The only hassle we have with this is how to store all the blocks we are producing. Cindy DuBois Stockton, CA --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From TillRenee <@t> uams.edu Tue May 8 13:36:38 2007 From: TillRenee <@t> uams.edu (Till, Renee) Date: Tue May 8 13:37:13 2007 Subject: [Histonet] protein block Message-ID: <11F927674DEBDC43B960809A7403C5D204550AA5@MAILPED.ad.uams.edu> I have a protocol from a paper that I need to try and reproduce. They used the Protein Block from Dako, along with the LSAB+ kit. The Block seems to no longer be available. What would be an alternative? Is this the same as the serum block I would normally use? Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72202 Lab (501)364-8504 Office Fax (501)364-3161 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From gcallis <@t> montana.edu Tue May 8 13:49:21 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue May 8 13:49:33 2007 Subject: [Histonet] protein block In-Reply-To: <11F927674DEBDC43B960809A7403C5D204550AA5@MAILPED.ad.uams.e du> References: <11F927674DEBDC43B960809A7403C5D204550AA5@MAILPED.ad.uams.edu> Message-ID: <6.0.0.22.1.20070508124600.01b06170@gemini.msu.montana.edu> Renee, I doubt it, sometimes protein blocks are serum-free blocks, and may contain BSA and/or casein. I would assume another company's block should do the job as well. Try Lab Vision, Biocare, etc to see what they have available. At 12:36 PM 5/8/2007, you wrote: >I have a protocol from a paper that I need to try and reproduce. They used >the Protein Block from Dako, along with the LSAB+ kit. The Block seems to no >longer be available. What would be an alternative? Is this the same as the >serum block I would normally use? > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From MMargiotta <@t> bmhmc.org Tue May 8 14:42:12 2007 From: MMargiotta <@t> bmhmc.org (Margiotta, Michele) Date: Tue May 8 14:42:15 2007 Subject: [Histonet] timer calibration Message-ID: <922CE5B88F398948B4076A9A4340E7AF036AF5FF@bmh_exchange.bmhmc.org> Hi All, Does anyone have a procedure for calibrating timers? We just had an inspection and got cited because our timers were not calibrated. Any info would be appreciated! Michele This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. From alaskagirl1950 <@t> yahoo.com Tue May 8 15:11:02 2007 From: alaskagirl1950 <@t> yahoo.com (Patricia Adams) Date: Tue May 8 15:11:06 2007 Subject: [Histonet] If you wanted an antibody what would it be? Message-ID: <102360.41177.qm@web52512.mail.re2.yahoo.com> First I wish to thank everyone for helping me with my species antibody problems. My question is for all of you other Vet School and Vet clinic people. Do you have problems finding certain types of antibodies that will react on certain species, if so what antibody and which species? I am trying to compile a list of the most needed antibodies that we need on the animal side of things. I do again wish to thank all who sent me help, I have been burning up the Internet looking up all the information sent my way. And trying to dodge my Pathologist and all his questions! (He knows all my hiding places ;(, bummer!). Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From rjbuesa <@t> yahoo.com Tue May 8 15:39:47 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue May 8 15:39:52 2007 Subject: [Histonet] timer calibration In-Reply-To: <922CE5B88F398948B4076A9A4340E7AF036AF5FF@bmh_exchange.bmhmc.org> Message-ID: <113979.14134.qm@web61212.mail.yahoo.com> Try any local watch repair store. They have to calibrate their repaired watches so it is very likely they can help you. Ren? J. "Margiotta, Michele" wrote: Hi All, Does anyone have a procedure for calibrating timers? We just had an inspection and got cited because our timers were not calibrated. Any info would be appreciated! Michele This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From mickie25 <@t> netzero.net Tue May 8 20:32:59 2007 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Tue May 8 20:33:05 2007 Subject: [SPAM] [Histonet] reprocessing paraffin blocks In-Reply-To: <6.0.0.22.1.20070504101611.01b0fe78@gemini.msu.montana.edu> References: <6.0.0.22.1.20070504101611.01b0fe78@gemini.msu.montana.edu> Message-ID: Hi Everyone. Thanks Gail for remembering my small article in Histo-Logic about 5 years ago! The procedure is very simple and the least harsh of any method I know. I did not develop it, but one of my former students brought it back from NSH and we used it with great success. First, melt the block down and blot off the excess paraffin from the tissue. Second, re-cassette the tissue in the same cassette, blotting excess paraffin from the cassette. Third, put the cassette in with the days normal tissue processing run, in formalin. Fourth, process as usual. The next morning, embed and cut. The fat (or under processed tissue will cut beautifully. The rational is that the well processed part of the block will have paraffin in it and will not feel the effects of dehydration. Xylene will melt out the paraffin and then paraffin will re-infiltrate this part. The unprocessed tissue areas are available to fix additionally and dehydrate, clear and infiltrate with paraffin. The net result is a reprocessed block with no harsh treatment and very little time expended to 'reprocess' by hand. It does take overnight to get the slides, but usually the pathologist will be happy to see slides he can read accurately. Hope this helps. Good Luck! Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Friday, May 04, 2007 9:18 AM To: Histonet@lists.utsouthwestern.edu Subject: [SPAM] [Histonet] reprocessing paraffin blocks Get this publication from Histo Logic on Sakura Finetek website. It was very simple and very little work overall. A Technique for Correcting Poorly Processed Paraffin Blocks. Michael L. Johnson, BS, HTL, HT(ASCP), Spokane, WA, May 2003;XXXVI(1):21. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.m.vanderloos <@t> amc.uva.nl Wed May 9 02:19:03 2007 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Wed May 9 02:19:23 2007 Subject: [Histonet] RE: Regulatory Tcells (FoxP3) IHC Message-ID: <132f4512faf0.12faf0132f45@amc.uva.nl> Melissa, We used the Abcam mouse antibody, clone 236A/E7 we great success on both human cryo and FFPE samples, and also in double staining with CD25, CD4 (cryo). For more details please see our paper recently accepted for JHC: Onno J. de Boer et al., Immunohistochemical Analysis of Regulatory T Cell Markers FOXP3 and GITR on CD4^+CD25^+ T Cells in Normal Skin and Inflammatory Dermatoses. Go to the JHC website ([1]www.jhc.org) and go to exPRESS. You will find our paper under: May 3rd 2007. Cheers, Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Mon, 7 May 2007 10:35:52 -0700 From: "Melissa Gonzalez" Subject: [Histonet] Regulatory Tcells (FoxP3) IHC To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi all, Anyone have any experience with this? How well does the Abcam antibody work on FFPE human tissues? What's a good positive control? Thanks Melissa References 1. http://www.jhc.org/ From c.m.vanderloos <@t> amc.uva.nl Wed May 9 02:50:37 2007 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Wed May 9 02:50:58 2007 Subject: [Histonet] RE: Leishmania Message-ID: Dear Sally, Last year I tried an antibody from Cedarlane Leishmania LPG, clone CA7AE (1:500, 60 min, RT). I could make it work with HIER citrate pH6.0 and indirect fluorescence on FFPE samples. Spectral imaging was used for unmixing "real" signal from autofluorescence. Even then it was hard to find the specific signal, due to some specific-looking nuclear background staining. The tissue samples that were positive with fluorescence were also subjected to IHC with anti-mouse polymers and DAB or LPR as chromogens. This totally failed and we gave up. I realize it's not a very hopeful story but perhaps it helps anyway. Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Mon, 7 May 2007 15:03:28 -0500 From: "Drew Sally A." Subject: [Histonet] Leishmania To: "Histonet" Could someone direct us to a place/person who might run an antibody against Leishmania? We have a pathologist asking questions about it, and all our usual source don't list it. Thank you! Sally Ann Drew, MT(ASCP) IHC/ISH Laboratory University of Wisconsin Hosp. & Clinics Madison, WI 53792 From Sherene.Cohen <@t> fccc.edu Wed May 9 08:30:05 2007 From: Sherene.Cohen <@t> fccc.edu (Cohen, Sherene B.) Date: Wed May 9 08:31:47 2007 Subject: [Histonet] FW: Her2 question Message-ID: <670B8345AF238F40910FEC4CA7D4B7D2F9E991@exchserver.fccc.edu> > -----Original Message----- > From: Cohen, Sherene B. > Sent: Tuesday, May 08, 2007 3:12 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: Her2 question > > Hey netters, > > My director just asked me about fixation in NBF and Her2. I'm curious to > know what others are doing about: > > A) Weekend processing of breast specimens > > B) If anyone has studied the antigenicity effect of these specimens > sitting in warm paraffin for at least 8 hours. > > Any feedeback is greatly appreciated. > > Sherene > > From rjbuesa <@t> yahoo.com Wed May 9 09:35:01 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 9 09:35:08 2007 Subject: [Histonet] FW: Her2 question In-Reply-To: <670B8345AF238F40910FEC4CA7D4B7D2F9E991@exchserver.fccc.edu> Message-ID: <222753.88085.qm@web61222.mail.yahoo.com> This was the subject of an extense discussion in Histonet just one month ago. It would be better for you to look in the archieves and benefit from that discussion. Ren? J. "Cohen, Sherene B." wrote: > -----Original Message----- > From: Cohen, Sherene B. > Sent: Tuesday, May 08, 2007 3:12 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: Her2 question > > Hey netters, > > My director just asked me about fixation in NBF and Her2. I'm curious to > know what others are doing about: > > A) Weekend processing of breast specimens > > B) If anyone has studied the antigenicity effect of these specimens > sitting in warm paraffin for at least 8 hours. > > Any feedeback is greatly appreciated. > > Sherene > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss an email again! Yahoo! Toolbar alerts you the instant new Mail arrives. Check it out. From jnocito <@t> satx.rr.com Mon May 14 10:08:30 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed May 9 10:08:32 2007 Subject: [Histonet] timer calibration References: <113979.14134.qm@web61212.mail.yahoo.com> Message-ID: <00c801c79639$bc0a91a0$d49eae18@yourxhtr8hvc4p> I'm sorry am I the only one that thinks calibrating timers is stupid. I mean how many histology procedures are so time sensitive that the timers have to be calibrated? Let's face it. I have had techs sit there and watch the clock and rinse as soon as the timer goes off. And I've had techs wait for the timer to go off , then mosey over and rinse. Both stains worked. I guess I'm just not anal enough. JTT ----- Original Message ----- From: "Rene J Buesa" To: "Margiotta, Michele" ; Sent: Tuesday, May 08, 2007 3:39 PM Subject: Re: [Histonet] timer calibration > Try any local watch repair store. They have to calibrate their repaired > watches so it is very likely they can help you. > Ren? J. > > "Margiotta, Michele" wrote: > Hi All, > > Does anyone have a procedure for calibrating timers? We just had an > inspection and got cited because our timers were not calibrated. Any info > would be appreciated! > > Michele > > > > This e-mail and any files transmitted with it are confidential and are > intended > solely for the use of the individual or entity to which they are > addressed. > This communication may contain material protected by the attorney-client > privilege. If you are not the intended recipient or the person responsible > for > delivering the e-mail to the intended recipient, be advised that you have > received > this e-mail in error and that any use, dissemination, forwarding, > printing, > or copying of this e-mail is strictly prohibited. If you have received > this e-mail in > error, please immediately notify the sender via return e-mail or call > Brookhaven Memorial Hospital Medical Center at (631) 654-7282. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Ahhh...imagining that irresistible "new car" smell? > Check outnew cars at Yahoo! Autos. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MLashus <@t> pathgroup.com Wed May 9 10:18:51 2007 From: MLashus <@t> pathgroup.com (Mighnon Lashus) Date: Wed May 9 10:18:57 2007 Subject: [Histonet] timer calibration Message-ID: <7DFAF4868AAAC34C986DF7E1AC16D02601156D54@pgnexchg1.pathgroup.com> Who sited you? Mighnon -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Monday, May 14, 2007 11:09 AM To: Rene J Buesa; Margiotta, Michele; histonet@pathology.swmed.edu Subject: Re: [Histonet] timer calibration I'm sorry am I the only one that thinks calibrating timers is stupid. I mean how many histology procedures are so time sensitive that the timers have to be calibrated? Let's face it. I have had techs sit there and watch the clock and rinse as soon as the timer goes off. And I've had techs wait for the timer to go off , then mosey over and rinse. Both stains worked. I guess I'm just not anal enough. JTT ----- Original Message ----- From: "Rene J Buesa" To: "Margiotta, Michele" ; Sent: Tuesday, May 08, 2007 3:39 PM Subject: Re: [Histonet] timer calibration > Try any local watch repair store. They have to calibrate their repaired > watches so it is very likely they can help you. > Ren? J. > > "Margiotta, Michele" wrote: > Hi All, > > Does anyone have a procedure for calibrating timers? We just had an > inspection and got cited because our timers were not calibrated. Any info > would be appreciated! > > Michele > > > > This e-mail and any files transmitted with it are confidential and are > intended > solely for the use of the individual or entity to which they are > addressed. > This communication may contain material protected by the attorney-client > privilege. If you are not the intended recipient or the person responsible > for > delivering the e-mail to the intended recipient, be advised that you have > received > this e-mail in error and that any use, dissemination, forwarding, > printing, > or copying of this e-mail is strictly prohibited. If you have received > this e-mail in > error, please immediately notify the sender via return e-mail or call > Brookhaven Memorial Hospital Medical Center at (631) 654-7282. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Ahhh...imagining that irresistible "new car" smell? > Check outnew cars at Yahoo! Autos. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc. immediately at 615-562-9255. From doug <@t> ppspath.com Wed May 9 11:25:31 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Wed May 9 10:24:14 2007 Subject: [Histonet] timer calibration In-Reply-To: <00c801c79639$bc0a91a0$d49eae18@yourxhtr8hvc4p> Message-ID: <1033394974-763687002@pathology.swmed.edu> Who inspects the calibrating timers? What are the calibrating timers calibrated against? What are the calibrating timers of the calibrating timers calibrated against? Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Monday, May 14, 2007 10:09 AM To: Rene J Buesa; Margiotta, Michele; histonet@pathology.swmed.edu Subject: Re: [Histonet] timer calibration I'm sorry am I the only one that thinks calibrating timers is stupid. I mean how many histology procedures are so time sensitive that the timers have to be calibrated? Let's face it. I have had techs sit there and watch the clock and rinse as soon as the timer goes off. And I've had techs wait for the timer to go off , then mosey over and rinse. Both stains worked. I guess I'm just not anal enough. JTT ----- Original Message ----- From: "Rene J Buesa" To: "Margiotta, Michele" ; Sent: Tuesday, May 08, 2007 3:39 PM Subject: Re: [Histonet] timer calibration > Try any local watch repair store. They have to calibrate their repaired > watches so it is very likely they can help you. > Ren? J. > > "Margiotta, Michele" wrote: > Hi All, > > Does anyone have a procedure for calibrating timers? We just had an > inspection and got cited because our timers were not calibrated. Any info > would be appreciated! > > Michele > > > > This e-mail and any files transmitted with it are confidential and are > intended > solely for the use of the individual or entity to which they are > addressed. > This communication may contain material protected by the attorney-client > privilege. If you are not the intended recipient or the person responsible > for > delivering the e-mail to the intended recipient, be advised that you have > received > this e-mail in error and that any use, dissemination, forwarding, > printing, > or copying of this e-mail is strictly prohibited. If you have received > this e-mail in > error, please immediately notify the sender via return e-mail or call > Brookhaven Memorial Hospital Medical Center at (631) 654-7282. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Ahhh...imagining that irresistible "new car" smell? > Check outnew cars at Yahoo! Autos. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pex0220 <@t> yahoo.com.cn Wed May 9 10:24:51 2007 From: pex0220 <@t> yahoo.com.cn (docqian) Date: Wed May 9 10:24:57 2007 Subject: [Histonet] Alizarin Red staining for mineralization nodules Message-ID: <628956.75919.qm@web15210.mail.cnb.yahoo.com> Dear all,=0A=0AI would like to perform Alizarin Red staining for mineraliza= tion nodules in primary osteoblasts, Does anyone have a protocol about it?= =0AIn addition, for mineralization nodule formation, we should add ascorbic= acid and beta-glycerolphosphate into the culture medium, my question is : = use which solution to dissovle these drugs (distilled water, or culture me= dium).=0A=0AThank you.=0A =0AGuofeng=0A=0A=0A ________________________= ___________________________________ =0A=C7=C0=D7=A2=D1=C5=BB=A2=C3=E2=B7=D1= =D3=CA=CF=E43.5G=C8=DD=C1=BF=A3=AC20M=B8=BD=BC=FE=A3=A1 =0Ahttp://cn.mail.y= ahoo.comFrom BBranton <@t> sarapath.com Wed May 9 10:30:39 2007 From: BBranton <@t> sarapath.com (Brian Branton) Date: Wed May 9 10:29:53 2007 Subject: [Histonet] Leica ASP300 Tissue Processor for sale Message-ID: <2A5183C7E289F646A4744C85841587BF04128A@timeclock.sarapath.com> Hello Histonet, We are selling one of our Leica ASP300 tissue processors. If anyone is interested, please checkout our eBay listing http://cgi.ebay.com/Leica-ASP300-Tissue-Processor_W0QQitemZ200106385547QQihZ010QQcategoryZ11816QQssPageNameZWDVWQQrdZ1QQcmdZViewItem. Thank You Brian Branton Purchasing Agent Sarasota Pathology (941) 362-8963 (941) 362-8964 FAX From JMitchell <@t> uwhealth.org Wed May 9 10:45:00 2007 From: JMitchell <@t> uwhealth.org (Mitchell Jean A.) Date: Wed May 9 10:45:10 2007 Subject: [Histonet] timer calibration In-Reply-To: <1033394974-763687002@pathology.swmed.edu> Message-ID: <936BDBD9AB6ED84FB1FD25FD55DCDFB1035585A2@uwhis-xchng4.uwhis.hosp.wisc.edu> JCAHO inspectors each and every time have inquired about my timer calibrations. I satisfy this requirement by annually calibrating my timers to my wall clock; with the wall clocks calibrated to Greenwich Mean Time (from a website) Jean Mitchell, BS HT (ASCP) University of Wisconsin Hospital & Clinics Neuromuscular Laboratory Manager 600 Highland Avenue Madison, WI 53792-5132 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Wednesday, May 09, 2007 11:26 AM To: 'Joe Nocito'; histonet@pathology.swmed.edu Subject: RE: [Histonet] timer calibration Who inspects the calibrating timers? What are the calibrating timers calibrated against? What are the calibrating timers of the calibrating timers calibrated against? Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 From ree3 <@t> leicester.ac.uk Wed May 9 10:58:38 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Wed May 9 10:58:47 2007 Subject: [Histonet] timer calibration In-Reply-To: <00c801c79639$bc0a91a0$d49eae18@yourxhtr8hvc4p> References: <00c801c79639$bc0a91a0$d49eae18@yourxhtr8hvc4p> Message-ID: Calibration of timers is essential, as one of the least publicised consequences of global warming is that time is speeding up by about a second a year( Wells et al;Journal of Man and Time Vol 6, 211-221, 2008). -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: 14 May 2007 16:09 To: Rene J Buesa; Margiotta, Michele; histonet@pathology.swmed.edu Subject: Re: [Histonet] timer calibration I'm sorry am I the only one that thinks calibrating timers is stupid. I mean how many histology procedures are so time sensitive that the timers have to be calibrated? Let's face it. I have had techs sit there and watch the clock and rinse as soon as the timer goes off. And I've had techs wait for the timer to go off , then mosey over and rinse. Both stains worked. I guess I'm just not anal enough. JTT ----- Original Message ----- From: "Rene J Buesa" To: "Margiotta, Michele" ; Sent: Tuesday, May 08, 2007 3:39 PM Subject: Re: [Histonet] timer calibration > Try any local watch repair store. They have to calibrate their > repaired watches so it is very likely they can help you. > Ren? J. > > "Margiotta, Michele" wrote: > Hi All, > > Does anyone have a procedure for calibrating timers? We just had an > inspection and got cited because our timers were not calibrated. Any > info would be appreciated! > > Michele > > > > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to which they > are addressed. > This communication may contain material protected by the > attorney-client privilege. If you are not the intended recipient or > the person responsible for delivering the e-mail to the intended > recipient, be advised that you have received this e-mail in error and > that any use, dissemination, forwarding, printing, or copying of this > e-mail is strictly prohibited. If you have received this e-mail in > error, please immediately notify the sender via return e-mail or call > Brookhaven Memorial Hospital Medical Center at (631) 654-7282. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Ahhh...imagining that irresistible "new car" smell? > Check outnew cars at Yahoo! Autos. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Wed May 9 11:34:31 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed May 9 11:34:33 2007 Subject: [Histonet] protein block In-Reply-To: <6.0.0.22.1.20070508124600.01b06170@gemini.msu.montana.edu> Message-ID: <002701c79257$ec227640$6501a8c0@Patsy> Dako's protein block is serum free and from what I understand is just casein, no bsa. I use serum free protein block from Open Biosystems, I also use their Stable Dab, Antibody Diluent, hematoxylin CS, etc., they are a lot cheaper from most and I like their products. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Tuesday, May 08, 2007 12:49 PM To: Till, Renee; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] protein block Renee, I doubt it, sometimes protein blocks are serum-free blocks, and may contain BSA and/or casein. I would assume another company's block should do the job as well. Try Lab Vision, Biocare, etc to see what they have available. At 12:36 PM 5/8/2007, you wrote: >I have a protocol from a paper that I need to try and reproduce. They used >the Protein Block from Dako, along with the LSAB+ kit. The Block seems to no >longer be available. What would be an alternative? Is this the same as the >serum block I would normally use? > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Wed May 9 11:40:13 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Wed May 9 11:40:26 2007 Subject: [Histonet] timer calibration In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C5C6@IS-E2K3.grhs.net> If we are going to ask if our timers are calibrated in the Histology Lab, then one could ask the question: "Do you calibrate the timers on your various automated instruments such as processors, stainers, cryostats, and the list could go on? I don't feel that 1 second a year is going to make a difference in my calibrated-timed tissue being processed or my H&E stains. If time does matter that precisely to you, then you most likely have a system in place for calibrating your timers. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, R.E. Sent: Wednesday, May 09, 2007 10:59 AM To: Joe Nocito; Rene J Buesa; Margiotta, Michele; histonet@pathology.swmed.edu Subject: RE: [Histonet] timer calibration Calibration of timers is essential, as one of the least publicised consequences of global warming is that time is speeding up by about a second a year( Wells et al;Journal of Man and Time Vol 6, 211-221, 2008). -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: 14 May 2007 16:09 To: Rene J Buesa; Margiotta, Michele; histonet@pathology.swmed.edu Subject: Re: [Histonet] timer calibration I'm sorry am I the only one that thinks calibrating timers is stupid. I mean how many histology procedures are so time sensitive that the timers have to be calibrated? Let's face it. I have had techs sit there and watch the clock and rinse as soon as the timer goes off. And I've had techs wait for the timer to go off , then mosey over and rinse. Both stains worked. I guess I'm just not anal enough. JTT ----- Original Message ----- From: "Rene J Buesa" To: "Margiotta, Michele" ; Sent: Tuesday, May 08, 2007 3:39 PM Subject: Re: [Histonet] timer calibration > Try any local watch repair store. They have to calibrate their > repaired watches so it is very likely they can help you. > Ren? J. > > "Margiotta, Michele" wrote: > Hi All, > > Does anyone have a procedure for calibrating timers? We just had an > inspection and got cited because our timers were not calibrated. Any > info would be appreciated! > > Michele > > > > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to which they > are addressed. > This communication may contain material protected by the > attorney-client privilege. If you are not the intended recipient or > the person responsible for delivering the e-mail to the intended > recipient, be advised that you have received this e-mail in error and > that any use, dissemination, forwarding, printing, or copying of this > e-mail is strictly prohibited. If you have received this e-mail in > error, please immediately notify the sender via return e-mail or call > Brookhaven Memorial Hospital Medical Center at (631) 654-7282. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Ahhh...imagining that irresistible "new car" smell? > Check outnew cars at Yahoo! Autos. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LisaKennedy <@t> catholichealth.net Wed May 9 11:41:06 2007 From: LisaKennedy <@t> catholichealth.net (Kennedy, Lisa) Date: Wed May 9 11:41:31 2007 Subject: [Histonet] (no subject) Message-ID: I am doing a search for information regarding Histologists who perform grossing in and dictation of surgical specimens. We have, in the past couple of years, begun to do that at our facility and are trying to get an idea of the increase of pay for histologist that doing this job warrants. Can anyone out there give me some pay ranges, etc. for Histologists who perform these tasks. Thanks so much. Lisa Kennedy, HT(ASCP) Good Samaritan Hospital Kearney, NE 68847 From bhewlett <@t> cogeco.ca Wed May 9 11:43:11 2007 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Wed May 9 11:43:13 2007 Subject: [Histonet] timer calibration References: <113979.14134.qm@web61212.mail.yahoo.com> <00c801c79639$bc0a91a0$d49eae18@yourxhtr8hvc4p> Message-ID: <002d01c79259$22348380$6500a8c0@mainbox> Joe, You are talking fundamental philosophical differences here. The inspectors are obviously Newtonian realists, while you're leaning more to the Leibnitz/Kant view. They are going to win! Bryan ----- Original Message ----- From: "Joe Nocito" To: "Rene J Buesa" ; "Margiotta, Michele" ; Sent: Monday, May 14, 2007 11:08 AM Subject: Re: [Histonet] timer calibration I'm sorry am I the only one that thinks calibrating timers is stupid. I mean how many histology procedures are so time sensitive that the timers have to be calibrated? Let's face it. I have had techs sit there and watch the clock and rinse as soon as the timer goes off. And I've had techs wait for the timer to go off , then mosey over and rinse. Both stains worked. I guess I'm just not anal enough. JTT ----- Original Message ----- From: "Rene J Buesa" To: "Margiotta, Michele" ; Sent: Tuesday, May 08, 2007 3:39 PM Subject: Re: [Histonet] timer calibration > Try any local watch repair store. They have to calibrate their repaired > watches so it is very likely they can help you. > Ren? J. > > "Margiotta, Michele" wrote: > Hi All, > > Does anyone have a procedure for calibrating timers? We just had an > inspection and got cited because our timers were not calibrated. Any info > would be appreciated! > > Michele > > > > This e-mail and any files transmitted with it are confidential and are > intended > solely for the use of the individual or entity to which they are > addressed. > This communication may contain material protected by the attorney-client > privilege. If you are not the intended recipient or the person responsible > for > delivering the e-mail to the intended recipient, be advised that you have > received > this e-mail in error and that any use, dissemination, forwarding, > printing, > or copying of this e-mail is strictly prohibited. If you have received > this e-mail in > error, please immediately notify the sender via return e-mail or call > Brookhaven Memorial Hospital Medical Center at (631) 654-7282. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Ahhh...imagining that irresistible "new car" smell? > Check outnew cars at Yahoo! Autos. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Wed May 9 11:50:35 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Wed May 9 11:50:44 2007 Subject: [Histonet] (no subject) In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C5C7@IS-E2K3.grhs.net> This is going to depend on the degree of grossing the tech does. If they only put GI type bx's in or if they do the entire gross-in of specimens to the degree a PA or Resident does. You may get a wide range of pay. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kennedy, Lisa Sent: Wednesday, May 09, 2007 11:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) I am doing a search for information regarding Histologists who perform grossing in and dictation of surgical specimens. We have, in the past couple of years, begun to do that at our facility and are trying to get an idea of the increase of pay for histologist that doing this job warrants. Can anyone out there give me some pay ranges, etc. for Histologists who perform these tasks. Thanks so much. Lisa Kennedy, HT(ASCP) Good Samaritan Hospital Kearney, NE 68847 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LisaKennedy <@t> catholichealth.net Wed May 9 12:04:04 2007 From: LisaKennedy <@t> catholichealth.net (Kennedy, Lisa) Date: Wed May 9 12:04:17 2007 Subject: [Histonet] (no subject) Message-ID: The specimens we gross in are GI biopsies plus appendix, gallbladders,some skins, tonsils that are gross only, cervical biopsies to include LEEPs, bursa's, lipomas, ganglions, prostate chips and such. Lisa Good Samaritan Hospital Kearney, NE 68847 From Lchausse <@t> nmh.org Wed May 9 12:23:38 2007 From: Lchausse <@t> nmh.org (Chaussey, Leslie) Date: Wed May 9 12:24:12 2007 Subject: [Histonet] (no subject) In-Reply-To: Message-ID: Our staff grosses similar specimens as below. However, they are not histotechs. They all have BS degrees in Biology or similar. One is a MT(ASCP). We have residents at our institution that currently do all the larger, more complex cases. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kennedy, Lisa Sent: Wednesday, May 09, 2007 12:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) The specimens we gross in are GI biopsies plus appendix, gallbladders,some skins, tonsils that are gross only, cervical biopsies to include LEEPs, bursa's, lipomas, ganglions, prostate chips and such. Lisa Good Samaritan Hospital Kearney, NE 68847 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- This message and any included attachments are intended only for the addressee. 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From Janet.Bonner <@t> FLHOSP.ORG Wed May 9 12:40:37 2007 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Wed May 9 12:41:20 2007 Subject: [Histonet] timer calibration References: <1033394974-763687002@pathology.swmed.edu> Message-ID: <5F31F38C96781A4FBE3196EBC22D47802E0F9E@fhosxchmb006.ADVENTISTCORP.NET> Calibrated Clock Satellites? ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Douglas D Deltour Sent: Wed 5/9/2007 12:25 PM To: 'Joe Nocito'; histonet@pathology.swmed.edu Subject: RE: [Histonet] timer calibration Who inspects the calibrating timers? What are the calibrating timers calibrated against? What are the calibrating timers of the calibrating timers calibrated against? Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Monday, May 14, 2007 10:09 AM To: Rene J Buesa; Margiotta, Michele; histonet@pathology.swmed.edu Subject: Re: [Histonet] timer calibration I'm sorry am I the only one that thinks calibrating timers is stupid. I mean how many histology procedures are so time sensitive that the timers have to be calibrated? Let's face it. I have had techs sit there and watch the clock and rinse as soon as the timer goes off. And I've had techs wait for the timer to go off , then mosey over and rinse. Both stains worked. I guess I'm just not anal enough. JTT ----- Original Message ----- From: "Rene J Buesa" To: "Margiotta, Michele" ; Sent: Tuesday, May 08, 2007 3:39 PM Subject: Re: [Histonet] timer calibration > Try any local watch repair store. They have to calibrate their repaired > watches so it is very likely they can help you. > Ren? J. > > "Margiotta, Michele" wrote: > Hi All, > > Does anyone have a procedure for calibrating timers? We just had an > inspection and got cited because our timers were not calibrated. Any info > would be appreciated! > > Michele > > > > This e-mail and any files transmitted with it are confidential and are > intended > solely for the use of the individual or entity to which they are > addressed. > This communication may contain material protected by the attorney-client > privilege. If you are not the intended recipient or the person responsible > for > delivering the e-mail to the intended recipient, be advised that you have > received > this e-mail in error and that any use, dissemination, forwarding, > printing, > or copying of this e-mail is strictly prohibited. If you have received > this e-mail in > error, please immediately notify the sender via return e-mail or call > Brookhaven Memorial Hospital Medical Center at (631) 654-7282. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Ahhh...imagining that irresistible "new car" smell? > Check outnew cars at Yahoo! Autos. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From Margaret.Perry <@t> sdstate.edu Wed May 9 12:42:29 2007 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Wed May 9 12:42:38 2007 Subject: [Histonet] timer calibration Message-ID: We calibrate our timers by doing the following. Use the telephone to call 303-499-7111. A voice will prompt you and tell you the time. At the minute turn on the timer and record the Coordinated Universal time and the timer time. Listen until the next minute and turn off the timer and record the Coordinated Universal Time and the time on the timer. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 From TJJ <@t> Stowers-Institute.org Wed May 9 12:45:50 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Wed May 9 12:46:14 2007 Subject: [Histonet] Casein block for phosphorylated antigens? Message-ID: Has anybody heard of (or can cite references for) not using casein to block in IHC when immunostaining for phosphorylated proteins? Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From tp2 <@t> medicine.wisc.edu Wed May 9 12:51:05 2007 From: tp2 <@t> medicine.wisc.edu (Thomas Pier) Date: Wed May 9 12:51:39 2007 Subject: [Histonet] TGF-B Message-ID: <4641C3B9020000DF000071AE@gwmail.medicine.wisc.edu> Hello, Has anybody out there had any success staining frozen human or rabbit tissue for TGF-B? If so, I'd appreciate any help you could give me. Tom Pier From TJJ <@t> Stowers-Institute.org Wed May 9 12:56:51 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Wed May 9 12:57:20 2007 Subject: [Histonet] Re: Casein block for phosphorylated antigens? Message-ID: I found this from a Google Search - http://www.nanotools.de/protocols/western_blot.php "2. Blocking of Nonspecific Binding Sites * Block blots for at least 2 hours with Blocking Solution. Blocking overnight is also possible (add phosphatase-inhibitors, e.g. sodiumfluoride, sodiumvanadate if the assay targets are phosphorylated epitopes). * Wash three times 5 min with 2x PBS/0.1% (v/v) Tween 20. General Blocking Solution We recommend a casein/Tween 20 based blocking solution (e.g. nanoTools product #3031-500/CPPT or #3031-3000/CPPT) for the use with phosphorylation-site specific antibodies and antibodies that are not directed to phosphorylated epitopes, unless otherwise stated in the datasheet. Alternative Blocking Solution This blocking solution is recommended for the use with phosphorylation-site specific antibodies that cannot be used with a casein based blocking solution. These are especially the phosphoserine/threonine-specific antibodies that are included in our Phosphoserine and Phosphothreonine Detection Kits (see list below). Phosphoserine-specific antibodies: PSER-1C8; -4A3; -4A9; -4H4; -7F12; -16B4 Phosphothreonine-specific antibodies: PTHR-1E11; -4D11; -14B3 For these antibodies, it is essential to avoid the use of blocking reagents containing phosphoproteins (e.g. milk or casein), since this will lead to competition of the highly phosphorylated casein with the phosphoserine-/ phosphothreonine-specific monoclonal antibodies. Application of casein or milk may lead to significant decrease or loss of signal intensity due to reaction of the primary antibody with the blocking/diluting agent. We therefore recommend to replace casein by 2% (w/v) BSA." This is on western blots. Does this also pertain to immunohistochemistry? Please, freely discuss opinions here! Teri > -----Original Message----- > From: Johnson, Teri > Sent: Wednesday, May 09, 2007 12:46 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: Casein block for phosphorylated antigens? > > Has anybody heard of (or can cite references for) not using casein to > block in IHC when immunostaining for phosphorylated proteins? > > Teri Johnson, HT(ASCP)QIHC > Managing Director Histology Facility > Stowers Institute for Medical Research > 1000 E. 50th St. > Kansas City, MO 64110 > From Shirley_PHUA <@t> hsa.gov.sg Wed May 9 13:06:52 2007 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Wed May 9 13:08:57 2007 Subject: [Histonet] Shirley is away 09 May 2007 afternoon ... Message-ID: I will be out of the office from 09-05-2007 to 10-05-2007. I will return on 10 May 2007. Pathologists : I will process your requests when I return. Otherwise, if urgent, please forward your mail to henry_kyaw@hsa.gov.sg Regrets for the inconveniences caused. From GDawson <@t> dynacaremilwaukee.com Wed May 9 13:12:06 2007 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Wed May 9 13:12:09 2007 Subject: [Histonet] timer calibration In-Reply-To: <00c801c79639$bc0a91a0$d49eae18@yourxhtr8hvc4p> Message-ID: Joe, You are NOT the only one who sees no value in this one. It truly boggles the mind that no one in the CAP has come to their senses and realized that so much of the process is absolute garbage. As we speak, there is a histo-lab recording temperatures, pH'ing all of their liquids religiously, monitoring humidity levels, calibrating their thermometers, pipettes, and clocks, as well as a multitude of other CAP enforced tasks and when all is said and done, their product (IHC, special stains, H&E's, in-situ, etc...) looks like garbage. At the same time, there is a histology manager who is working 11 hour days that is down 2 FTE's that has been forced to prioritize that getting the clinical workload out is far more important than the above mentioned tasks. Every day is a challenge and, although he or she cannot perform these CAP required things religiously, the end product looks great. Fast forward to the CAP inpections for these two labs and cringe along with me as the first lab that produces an inferior products sails through their inspection with flying colors while the second lab is repeatedly cited and must put in even more overtime to "correct" their gross CAP neglegence. I believe that CAP inspections need to put way more effort into pulling examples of the finished product and checking it for acceptable quality and less time on making sure our timers aren't off by a tenth of a second per every minute counted. Well, I must go since our Artel reagents have been delivered and I can finally calibrate the equipment used to calibrate my pipettes...can't wait. Done Venting, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Joe Nocito Sent: Monday, May 14, 2007 10:09 AM To: Rene J Buesa; Margiotta, Michele; histonet@pathology.swmed.edu Subject: Re: [Histonet] timer calibration I'm sorry am I the only one that thinks calibrating timers is stupid. I mean how many histology procedures are so time sensitive that the timers have to be calibrated? Let's face it. I have had techs sit there and watch the clock and rinse as soon as the timer goes off. And I've had techs wait for the timer to go off , then mosey over and rinse. Both stains worked. I guess I'm just not anal enough. JTT ----- Original Message ----- From: "Rene J Buesa" To: "Margiotta, Michele" ; Sent: Tuesday, May 08, 2007 3:39 PM Subject: Re: [Histonet] timer calibration > Try any local watch repair store. They have to calibrate their repaired > watches so it is very likely they can help you. > Ren? J. > > "Margiotta, Michele" wrote: > Hi All, > > Does anyone have a procedure for calibrating timers? We just had an > inspection and got cited because our timers were not calibrated. Any info > would be appreciated! > > Michele > > > > This e-mail and any files transmitted with it are confidential and are > intended > solely for the use of the individual or entity to which they are > addressed. > This communication may contain material protected by the attorney-client > privilege. If you are not the intended recipient or the person responsible > for > delivering the e-mail to the intended recipient, be advised that you have > received > this e-mail in error and that any use, dissemination, forwarding, > printing, > or copying of this e-mail is strictly prohibited. If you have received > this e-mail in > error, please immediately notify the sender via return e-mail or call > Brookhaven Memorial Hospital Medical Center at (631) 654-7282. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Ahhh...imagining that irresistible "new car" smell? > Check outnew cars at Yahoo! Autos. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charles.Embrey <@t> carle.com Wed May 9 13:29:23 2007 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Wed May 9 13:29:29 2007 Subject: [Histonet] (no subject) In-Reply-To: Message-ID: <44780C571F28624DBB446DE55C4D733A1FE4DE@EXCHANGEBE1.carle.com> Be very careful telling folks that you do gross only specimens. This is a very big subject that has been discussed through the PA sites. It is legally fraud to bill for something that you do not do. If the technician or PA does the gross only exam and the pathologists bill for the professional component then it is fraud. Grossing what you say you are grossing falls under "grossing" even in CAP watered down definition and counts as High complexity testing. The technicians doing that service should be compensated as such and should be paid close to what a PA would make. Charles Embrey, PA(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kennedy, Lisa Sent: Wednesday, May 09, 2007 12:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) The specimens we gross in are GI biopsies plus appendix, gallbladders,some skins, tonsils that are gross only, cervical biopsies to include LEEPs, bursa's, lipomas, ganglions, prostate chips and such. Lisa Good Samaritan Hospital Kearney, NE 68847 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From buckeyedermatology <@t> yahoo.com Wed May 9 13:53:28 2007 From: buckeyedermatology <@t> yahoo.com (J. Cruz) Date: Wed May 9 13:53:31 2007 Subject: [Histonet] job opportunity Message-ID: <660261.29354.qm@web33202.mail.mud.yahoo.com> My name is Andrew I work with Buckeye Dermatology her in Columbus,Oh and we are looking for day shift histologist. You can e-mail your resume to Buckeye Dermatology @ yahoo. com --------------------------------- 8:00? 8:25? 8:40? Find a flick in no time with theYahoo! Search movie showtime shortcut. From CIngles <@t> uwhealth.org Wed May 9 14:02:14 2007 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Wed May 9 14:02:18 2007 Subject: [Histonet] timer calibration References: Message-ID: <08A0A863637F1349BBFD83A96B27A50A12003E@uwhis-xchng3.uwhis.hosp.wisc.edu> Why don't we all just get atomic clocks and be done with it. I don't believe they ever need to be calibrated. (unless the laws of physics and radioactive element half-lives suddenly change). I agree with Joe. Staining is a special talent anyway. I have had to reset timers to add more incubation time on stains lots of times (especially silver). Oh sorry, it's only Wednesday. Only two more days to go. Claire Ingles UW Hospital Madison WI ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Perry, Margaret Sent: Wed 5/9/2007 12:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] timer calibration We calibrate our timers by doing the following. Use the telephone to call 303-499-7111. A voice will prompt you and tell you the time. At the minute turn on the timer and record the Coordinated Universal time and the timer time. Listen until the next minute and turn off the timer and record the Coordinated Universal Time and the time on the timer. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LisaKennedy <@t> catholichealth.net Wed May 9 14:03:35 2007 From: LisaKennedy <@t> catholichealth.net (Kennedy, Lisa) Date: Wed May 9 14:03:46 2007 Subject: [Histonet] gross error Message-ID: I am so sorry, I mistakingly said we did gross only on tonsils, what I meant to say was we do gross on tonsils that require a microscopic examination to be performed by the pathologist. My mistake. Lisa Kennedy From lblazek <@t> digestivespecialists.com Wed May 9 14:11:08 2007 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed May 9 14:10:10 2007 Subject: [Histonet] timer calibration In-Reply-To: <08A0A863637F1349BBFD83A96B27A50A12003E@uwhis-xchng3.uwhis.hosp.wisc.edu> References: <08A0A863637F1349BBFD83A96B27A50A12003E@uwhis-xchng3.uwhis.hosp.wisc.edu> Message-ID: <1F937FB30BDB7C4A9F39F83FEA8D379F07EE44@bruexchange1.digestivespecialists.com> I can't figure out how to calibrate the timer that lives in my head. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Wednesday, May 09, 2007 3:02 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] timer calibration Why don't we all just get atomic clocks and be done with it. I don't believe they ever need to be calibrated. (unless the laws of physics and radioactive element half-lives suddenly change). I agree with Joe. Staining is a special talent anyway. I have had to reset timers to add more incubation time on stains lots of times (especially silver). Oh sorry, it's only Wednesday. Only two more days to go. Claire Ingles UW Hospital Madison WI ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Perry, Margaret Sent: Wed 5/9/2007 12:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] timer calibration We calibrate our timers by doing the following. Use the telephone to call 303-499-7111. A voice will prompt you and tell you the time. At the minute turn on the timer and record the Coordinated Universal time and the timer time. Listen until the next minute and turn off the timer and record the Coordinated Universal Time and the time on the timer. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> flhosp.org Wed May 9 14:12:43 2007 From: Janet.Bonner <@t> flhosp.org (Bonner, Janet) Date: Wed May 9 14:13:31 2007 Subject: [Histonet] timer calibration References: <08A0A863637F1349BBFD83A96B27A50A12003E@uwhis-xchng3.uwhis.hosp.wisc.edu> Message-ID: <5F31F38C96781A4FBE3196EBC22D47802E0FA6@fhosxchmb006.ADVENTISTCORP.NET> The Atomic clock we had was great until the battery ran down! ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Ingles Claire Sent: Wed 5/9/2007 3:02 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] timer calibration Why don't we all just get atomic clocks and be done with it. I don't believe they ever need to be calibrated. (unless the laws of physics and radioactive element half-lives suddenly change). I agree with Joe. Staining is a special talent anyway. I have had to reset timers to add more incubation time on stains lots of times (especially silver). Oh sorry, it's only Wednesday. Only two more days to go. Claire Ingles UW Hospital Madison WI ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Perry, Margaret Sent: Wed 5/9/2007 12:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] timer calibration We calibrate our timers by doing the following. Use the telephone to call 303-499-7111. A voice will prompt you and tell you the time. At the minute turn on the timer and record the Coordinated Universal time and the timer time. Listen until the next minute and turn off the timer and record the Coordinated Universal Time and the time on the timer. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From Jackie.O'Connor <@t> abbott.com Wed May 9 14:15:37 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed May 9 14:16:11 2007 Subject: [Histonet] timer calibration In-Reply-To: <1F937FB30BDB7C4A9F39F83FEA8D379F07EE44@bruexchange1.digestivespecialists.com> Message-ID: Just listen to it's little voice - like I do. Bwa ha ha! "Blazek, Linda" Sent by: histonet-bounces@lists.utsouthwestern.edu 05/09/2007 02:11 PM To "Ingles Claire" , cc Subject RE: [Histonet] timer calibration I can't figure out how to calibrate the timer that lives in my head. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Wednesday, May 09, 2007 3:02 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] timer calibration Why don't we all just get atomic clocks and be done with it. I don't believe they ever need to be calibrated. (unless the laws of physics and radioactive element half-lives suddenly change). I agree with Joe. Staining is a special talent anyway. I have had to reset timers to add more incubation time on stains lots of times (especially silver). Oh sorry, it's only Wednesday. Only two more days to go. Claire Ingles UW Hospital Madison WI ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Perry, Margaret Sent: Wed 5/9/2007 12:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] timer calibration We calibrate our timers by doing the following. Use the telephone to call 303-499-7111. A voice will prompt you and tell you the time. At the minute turn on the timer and record the Coordinated Universal time and the timer time. Listen until the next minute and turn off the timer and record the Coordinated Universal Time and the time on the timer. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Wed May 9 14:19:20 2007 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed May 9 14:18:14 2007 Subject: [Histonet] timer calibration In-Reply-To: References: <1F937FB30BDB7C4A9F39F83FEA8D379F07EE44@bruexchange1.digestivespecialists.com> Message-ID: <1F937FB30BDB7C4A9F39F83FEA8D379F07EE45@bruexchange1.digestivespecialists.com> But I'm afraid if I start trying to calibrate them they will start arguing over the .5 second that one may differ from the other. Did someone say it was Friday!!? ________________________________ From: Jackie M O'Connor [mailto:Jackie.O'Connor@abbott.com] Sent: Wednesday, May 09, 2007 3:16 PM To: Blazek, Linda Cc: Ingles Claire; histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] timer calibration Just listen to it's little voice - like I do. Bwa ha ha! "Blazek, Linda" Sent by: histonet-bounces@lists.utsouthwestern.edu 05/09/2007 02:11 PM To "Ingles Claire" , cc Subject RE: [Histonet] timer calibration I can't figure out how to calibrate the timer that lives in my head. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Wednesday, May 09, 2007 3:02 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] timer calibration Why don't we all just get atomic clocks and be done with it. I don't believe they ever need to be calibrated. (unless the laws of physics and radioactive element half-lives suddenly change). I agree with Joe. Staining is a special talent anyway. I have had to reset timers to add more incubation time on stains lots of times (especially silver). Oh sorry, it's only Wednesday. Only two more days to go. Claire Ingles UW Hospital Madison WI ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Perry, Margaret Sent: Wed 5/9/2007 12:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] timer calibration We calibrate our timers by doing the following. Use the telephone to call 303-499-7111. A voice will prompt you and tell you the time. At the minute turn on the timer and record the Coordinated Universal time and the timer time. Listen until the next minute and turn off the timer and record the Coordinated Universal Time and the time on the timer. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From timothy.macatee <@t> med.nyu.edu Wed May 9 14:24:20 2007 From: timothy.macatee <@t> med.nyu.edu (Timothy Macatee) Date: Wed May 9 14:27:30 2007 Subject: [Histonet] timer calibration In-Reply-To: <1F937FB30BDB7C4A9F39F83FEA8D379F07EE45@bruexchange1.digestivespecialists.com> Message-ID: Here I just got this calibration off the web.... 1 2 3 4 5 6 7 8 9 10 On 5/9/07 3:19 PM, "Blazek, Linda" wrote: > But I'm afraid if I start trying to calibrate them they will start > arguing over the .5 second that one may differ from the other. Did > someone say it was Friday!!? > > > > > > ________________________________ > > From: Jackie M O'Connor [mailto:Jackie.O'Connor@abbott.com] > Sent: Wednesday, May 09, 2007 3:16 PM > To: Blazek, Linda > Cc: Ingles Claire; histonet@lists.utsouthwestern.edu; > histonet-bounces@lists.utsouthwestern.edu > Subject: RE: [Histonet] timer calibration > > > > > Just listen to it's little voice - like I do. Bwa ha ha! > > > > > > "Blazek, Linda" > Sent by: histonet-bounces@lists.utsouthwestern.edu > > 05/09/2007 02:11 PM > > To > > "Ingles Claire" , > > > cc > > > > Subject > > RE: [Histonet] timer calibration > > > > > > > > > > > I can't figure out how to calibrate the timer that lives in my head. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles > Claire > Sent: Wednesday, May 09, 2007 3:02 PM > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] timer calibration > > > Why don't we all just get atomic clocks and be done with it. I don't > believe they ever need to be calibrated. (unless the laws of physics and > radioactive element half-lives suddenly change). I agree with Joe. > Staining is a special talent anyway. I have had to reset timers to add > more incubation time on stains lots of times (especially silver). Oh > sorry, it's only Wednesday. Only two more days to go. > > Claire Ingles > UW Hospital > Madison WI > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Perry, > Margaret > Sent: Wed 5/9/2007 12:42 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] timer calibration > > > > We calibrate our timers by doing the following. Use the telephone to > call 303-499-7111. A voice will prompt you and tell you the time. At > the minute turn on the timer and record the Coordinated Universal time > and the timer time. Listen until the next minute and turn off the timer > and record the Coordinated Universal Time and the time on the timer. > > > > > > Margaret Perry HT (ASCP) > > IHC Lab Manager Veterinary Science > > Animal Disease Research and Diagnostic Lab > > South Dakota State University > > Box 2175 North Campus Drive > > Brookings SD 57007 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Tim Macatee Research Histology Core New York University School of Medicine 550 First Ave. Department of Pathology. Medical Science Building - Room 521 New York, N.Y. 10016 (212) 263-3888 From buckeyedermatology <@t> yahoo.com Wed May 9 14:55:28 2007 From: buckeyedermatology <@t> yahoo.com (J. Cruz) Date: Wed May 9 14:55:32 2007 Subject: [Histonet] job opportunity Message-ID: <650412.53020.qm@web33210.mail.mud.yahoo.com> My name is Andrew I work with Buckeye Dermatology her in Columbus,Oh and we are looking for day shift histologist. You can e-mail your resume to Buckeye Dermatology @ yahoo. com --------------------------------- Expecting? Get great news right away with email Auto-Check. Try the Yahoo! Mail Beta. From gcallis <@t> montana.edu Wed May 9 14:56:28 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed May 9 14:56:38 2007 Subject: [Histonet] Interesting discussion about RE: timer calibration In-Reply-To: <08A0A863637F1349BBFD83A96B27A50A12003E@uwhis-xchng3.uwhis. hosp.wisc.edu> References: <08A0A863637F1349BBFD83A96B27A50A12003E@uwhis-xchng3.uwhis.hosp.wisc.edu> Message-ID: <6.0.0.22.1.20070509132552.01b40ae8@gemini.msu.montana.edu> I have followed this discussion with interest and a bit of humor on the rationale for this chore. It escapes me a bit - but I did like the idea of an atomic clock but then saw the reply about battery failure. Also, Greenwich time seemed the most logical over-all, always available and correct. I was most bothered by a second added onto every year, and that means I get older by the second annually. Hmmmm - but then the need for calibrated timers for validation purposes with complex testing? Is it the automated stainers/processors they are worried about? As for resetting timers to do staining, my eyes are the timer when developing a chromogen during manual IHC procedures - this is controlled with a microscope. I have let a clock run up in time to garner a "ballpark" figure for optimal development, and found that can vary from day to day too, not an exact timing to be sure. I agree with Claire on silver staining, but depend on microscopic examination to monitor silver (for finalizing GMS, Jones methenamine silver) ,and other proper removal of dyes Luxol fast blue, decolorizing tissue Gram stain. Now for a question: Do you think CAP will ever require eye calibration? Sorry to even suggest that - Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 At 01:02 PM 5/9/2007, you wrote: > >Why don't we all just get atomic clocks and be done with it. I don't >believe they ever need to be calibrated. (unless the laws of physics and >radioactive element half-lives suddenly change). I agree with Joe. >Staining is a special talent anyway. I have had to reset timers to add >more incubation time on stains lots of times (especially silver). Oh >sorry, it's only Wednesday. Only two more days to go. > >Claire Ingles > UW Hospital >Madison WI > >________________________________ > >From: histonet-bounces@lists.utsouthwestern.edu on behalf of Perry, Margaret >Sent: Wed 5/9/2007 12:42 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] timer calibration > > > >We calibrate our timers by doing the following. Use the telephone to >call 303-499-7111. A voice will prompt you and tell you the time. At >the minute turn on the timer and record the Coordinated Universal time >and the timer time. Listen until the next minute and turn off the timer >and record the Coordinated Universal Time and the time on the timer. > > > > > >Margaret Perry HT (ASCP) > >IHC Lab Manager Veterinary Science > >Animal Disease Research and Diagnostic Lab > >South Dakota State University > >Box 2175 North Campus Drive > >Brookings SD 57007 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Wed May 9 15:01:04 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed May 9 15:01:47 2007 Subject: [Histonet] Interesting discussion about RE: timer calibration In-Reply-To: <6.0.0.22.1.20070509132552.01b40ae8@gemini.msu.montana.edu> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FF97CF@sjhaexc02.sjha.org> I believe this calibration has been on the general check list since back in the 80s. I remember how silly we thought it was, most inspectors don't pay attention in AP. I think it is more critical in the clinical lab. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Gayle Callis Sent: Wednesday, May 09, 2007 3:56 PM To: Ingles Claire; Histonet@lists.utsouthwestern.edu Subject: [Histonet] Interesting discussion about RE: timer calibration I have followed this discussion with interest and a bit of humor on the rationale for this chore. It escapes me a bit - but I did like the idea of an atomic clock but then saw the reply about battery failure. Also, Greenwich time seemed the most logical over-all, always available and correct. I was most bothered by a second added onto every year, and that means I get older by the second annually. Hmmmm - but then the need for calibrated timers for validation purposes with complex testing? Is it the automated stainers/processors they are worried about? As for resetting timers to do staining, my eyes are the timer when developing a chromogen during manual IHC procedures - this is controlled with a microscope. I have let a clock run up in time to garner a "ballpark" figure for optimal development, and found that can vary from day to day too, not an exact timing to be sure. I agree with Claire on silver staining, but depend on microscopic examination to monitor silver (for finalizing GMS, Jones methenamine silver) ,and other proper removal of dyes Luxol fast blue, decolorizing tissue Gram stain. Now for a question: Do you think CAP will ever require eye calibration? Sorry to even suggest that - Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 At 01:02 PM 5/9/2007, you wrote: > >Why don't we all just get atomic clocks and be done with it. I don't >believe they ever need to be calibrated. (unless the laws of physics and >radioactive element half-lives suddenly change). I agree with Joe. >Staining is a special talent anyway. I have had to reset timers to add >more incubation time on stains lots of times (especially silver). Oh >sorry, it's only Wednesday. Only two more days to go. > >Claire Ingles > UW Hospital >Madison WI > >________________________________ > >From: histonet-bounces@lists.utsouthwestern.edu on behalf of Perry, Margaret >Sent: Wed 5/9/2007 12:42 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] timer calibration > > > >We calibrate our timers by doing the following. Use the telephone to >call 303-499-7111. A voice will prompt you and tell you the time. At >the minute turn on the timer and record the Coordinated Universal time >and the timer time. Listen until the next minute and turn off the timer >and record the Coordinated Universal Time and the time on the timer. > > > > > >Margaret Perry HT (ASCP) > >IHC Lab Manager Veterinary Science > >Animal Disease Research and Diagnostic Lab > >South Dakota State University > >Box 2175 North Campus Drive > >Brookings SD 57007 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From pmarcum <@t> vet.upenn.edu Wed May 9 15:06:27 2007 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Wed May 9 15:06:34 2007 Subject: [Histonet] Interesting discussion about RE: timer calibration In-Reply-To: <6.0.0.22.1.20070509132552.01b40ae8@gemini.msu.montana.edu> References: <08A0A863637F1349BBFD83A96B27A50A12003E@uwhis-xchng3.uwhis.hosp.wisc.edu> <6.0.0.22.1.20070509132552.01b40ae8@gemini.msu.montana.edu> Message-ID: <6.2.5.6.2.20070509160345.01c2f5d0@vet.upenn.edu> Shame on you Gayle!! Now they will attempt to calibrate eyes and we are in trouble 'cause they will have to decide with or without glasses and/or contacts, not to mention the colour charts to be sure we know which shade of blue or purple or red is correct. Oh this is frightening!! Let's not tell ATF about recycling either. Gaining that second year to get older does scare me a little. Pam At 03:56 PM 5/9/2007, Gayle Callis wrote: >I have followed this discussion with interest and a bit of humor on >the rationale for this chore. It escapes me a bit - but I did like >the idea of an atomic clock but then saw the reply about battery >failure. Also, Greenwich time seemed the most logical over-all, >always available and correct. I was most bothered by a second added >onto every year, and that means I get older by the second >annually. Hmmmm - but then the need for calibrated timers for >validation purposes with complex testing? Is it the automated >stainers/processors they are worried about? > >As for resetting timers to do staining, my eyes are the timer when >developing a chromogen during manual IHC procedures - this >is controlled with a microscope. I have let a clock run up in time >to garner a "ballpark" figure for optimal development, and found >that can vary from day to day too, not an exact timing to be sure. > >I agree with Claire on silver staining, but depend on microscopic >examination to monitor silver (for finalizing GMS, Jones methenamine >silver) ,and other proper removal of dyes Luxol fast blue, >decolorizing tissue Gram stain. > >Now for a question: Do you think CAP will ever require eye >calibration? Sorry to even suggest that - > >Gayle Callis HTL, HT, MT(ASCP) >Research Histopathology Supervisor >Veterinary Molecular Biology >Montana State University >Bozeman MT 59717 > > > At 01:02 PM 5/9/2007, you wrote: >> >>Why don't we all just get atomic clocks and be done with it. I >>don't believe they ever need to be calibrated. (unless the laws of >>physics and radioactive element half-lives suddenly change). I >>agree with Joe. Staining is a special talent anyway. I have had to >>reset timers to add more incubation time on stains lots of times >>(especially silver). Oh sorry, it's only Wednesday. Only two more days to go. >> >>Claire Ingles >> UW Hospital >>Madison WI >> >>________________________________ >> >>From: histonet-bounces@lists.utsouthwestern.edu on behalf of Perry, Margaret >>Sent: Wed 5/9/2007 12:42 PM >>To: histonet@lists.utsouthwestern.edu >>Subject: [Histonet] timer calibration >> >> >> >>We calibrate our timers by doing the following. Use the telephone to >>call 303-499-7111. A voice will prompt you and tell you the time. At >>the minute turn on the timer and record the Coordinated Universal time >>and the timer time. Listen until the next minute and turn off the timer >>and record the Coordinated Universal Time and the time on the timer. >> >> >> >> >> >>Margaret Perry HT (ASCP) >> >>IHC Lab Manager Veterinary Science >> >>Animal Disease Research and Diagnostic Lab >> >>South Dakota State University >> >>Box 2175 North Campus Drive >> >>Brookings SD 57007 >> >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From holliday.casey <@t> gmail.com Wed May 9 15:22:12 2007 From: holliday.casey <@t> gmail.com (Casey Holliday) Date: Wed May 9 15:22:17 2007 Subject: [Histonet] MMA embedding services Message-ID: Hi Histonet, Are there any recommended firms that offer MMA embedding and sectioning? Thanks -- Casey Holliday Joan C Edwards School of Medicine Marshall University From oshel1pe <@t> cmich.edu Wed May 9 15:23:26 2007 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Wed May 9 15:23:38 2007 Subject: [Histonet] Interesting discussion about RE: timer calibration In-Reply-To: <6.0.0.22.1.20070509132552.01b40ae8@gemini.msu.montana.edu> References: <08A0A863637F1349BBFD83A96B27A50A12003E@uwhis-xchng3.uwhis.hosp.wisc.edu> <6.0.0.22.1.20070509132552.01b40ae8@gemini.msu.montana.edu> Message-ID: Sure, why not? Require all histotechs to pass a "color-blindness" (note the quotes) eye exam. Given that women generally have better color discrimination than men in the red-green end of the spectrum, female histotechs should be more valuble and able to demand higher pay. I can just see medical supply houses all carrying Pantone color charts ... Phil >I have followed this discussion with interest and a bit of humor on >the rationale for this chore. It escapes me a bit - but I did like >the idea of an atomic clock but then saw the reply about battery >failure. Also, Greenwich time seemed the most logical over-all, >always available and correct. I was most bothered by a second added >onto every year, and that means I get older by the second annually. >Hmmmm - but then the need for calibrated timers for validation >purposes with complex testing? Is it the automated >stainers/processors they are worried about? > >As for resetting timers to do staining, my eyes are the timer when >developing a chromogen during manual IHC procedures - this is >controlled with a microscope. I have let a clock run up in time to >garner a "ballpark" figure for optimal development, and found that >can vary from day to day too, not an exact timing to be sure. > >I agree with Claire on silver staining, but depend on microscopic >examination to monitor silver (for finalizing GMS, Jones methenamine >silver) ,and other proper removal of dyes Luxol fast blue, >decolorizing tissue Gram stain. > >Now for a question: Do you think CAP will ever require eye >calibration? Sorry to even suggest that - > >Gayle Callis HTL, HT, MT(ASCP) >Research Histopathology Supervisor >Veterinary Molecular Biology >Montana State University >Bozeman MT 59717 > > > At 01:02 PM 5/9/2007, you wrote: >> >>Why don't we all just get atomic clocks and be done with it. I >>don't believe they ever need to be calibrated. (unless the laws of >>physics and radioactive element half-lives suddenly change). I >>agree with Joe. Staining is a special talent anyway. I have had to >>reset timers to add more incubation time on stains lots of times >>(especially silver). Oh sorry, it's only Wednesday. Only two more >>days to go. >> >>Claire Ingles >> UW Hospital >>Madison WI >> >>________________________________ >> >>From: histonet-bounces@lists.utsouthwestern.edu on behalf of Perry, Margaret >>Sent: Wed 5/9/2007 12:42 PM >>To: histonet@lists.utsouthwestern.edu >>Subject: [Histonet] timer calibration >> >> >> >>We calibrate our timers by doing the following. Use the telephone to >>call 303-499-7111. A voice will prompt you and tell you the time. At >>the minute turn on the timer and record the Coordinated Universal time >>and the timer time. Listen until the next minute and turn off the timer >>and record the Coordinated Universal Time and the time on the timer. >> >> >> >> >> >>Margaret Perry HT (ASCP) >> >>IHC Lab Manager Veterinary Science >> >>Animal Disease Research and Diagnostic Lab >> >>South Dakota State University >> >>Box 2175 North Campus Drive >> >>Brookings SD 57007 -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 voice: (989) 774-3576 dept. fax: (989) 774-3462 From bhewlett <@t> cogeco.ca Wed May 9 15:25:08 2007 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Wed May 9 15:25:10 2007 Subject: [Histonet] Interesting discussion about RE: timer calibration References: <08A0A863637F1349BBFD83A96B27A50A12003E@uwhis-xchng3.uwhis.hosp.wisc.edu><6.0.0.22.1.20070509132552.01b40ae8@gemini.msu.montana.edu> <6.2.5.6.2.20070509160345.01c2f5d0@vet.upenn.edu> Message-ID: <008c01c79278$23810fa0$6500a8c0@mainbox> So Joe isn't the only follower of Leibnitz/Kant, I suspected as much! Gayle, a mere second a year is nothing to us folks, I can't see those fine divisions on the timer anyway. You might want to try counting instead, one thousand and one .. one thousand and two etc. I've been doing that to time stains for years, the only downside is that colleagues who overhear, think you are losing it! Bryan ----- Original Message ----- From: "Pamela Marcum" To: "Gayle Callis" ; "Ingles Claire" ; Sent: Wednesday, May 09, 2007 4:06 PM Subject: Re: [Histonet] Interesting discussion about RE: timer calibration > Shame on you Gayle!! Now they will attempt to calibrate eyes and we are > in trouble 'cause they will have to decide with or without glasses and/or > contacts, not to mention the colour charts to be sure we know which shade > of blue or purple or red is correct. Oh this is frightening!! > > Let's not tell ATF about recycling either. > > Gaining that second year to get older does scare me a little. > > Pam > > At 03:56 PM 5/9/2007, Gayle Callis wrote: >>I have followed this discussion with interest and a bit of humor on the >>rationale for this chore. It escapes me a bit - but I did like the idea >>of an atomic clock but then saw the reply about battery failure. Also, >>Greenwich time seemed the most logical over-all, always available and >>correct. I was most bothered by a second added onto every year, and that >>means I get older by the second annually. Hmmmm - but then the need for >>calibrated timers for validation purposes with complex testing? Is it the >>automated stainers/processors they are worried about? >> >>As for resetting timers to do staining, my eyes are the timer when >>developing a chromogen during manual IHC procedures - this is controlled >>with a microscope. I have let a clock run up in time to garner a >>"ballpark" figure for optimal development, and found that can vary from >>day to day too, not an exact timing to be sure. >> >>I agree with Claire on silver staining, but depend on microscopic >>examination to monitor silver (for finalizing GMS, Jones methenamine >>silver) ,and other proper removal of dyes Luxol fast blue, decolorizing >>tissue Gram stain. >> >>Now for a question: Do you think CAP will ever require eye calibration? >>Sorry to even suggest that - >> >>Gayle Callis HTL, HT, MT(ASCP) >>Research Histopathology Supervisor >>Veterinary Molecular Biology >>Montana State University >>Bozeman MT 59717 >> >> >> At 01:02 PM 5/9/2007, you wrote: >>> >>>Why don't we all just get atomic clocks and be done with it. I don't >>>believe they ever need to be calibrated. (unless the laws of physics and >>>radioactive element half-lives suddenly change). I agree with Joe. >>>Staining is a special talent anyway. I have had to reset timers to add >>>more incubation time on stains lots of times (especially silver). Oh >>>sorry, it's only Wednesday. Only two more days to go. >>> >>>Claire Ingles >>> UW Hospital >>>Madison WI >>> >>>________________________________ >>> >>>From: histonet-bounces@lists.utsouthwestern.edu on behalf of Perry, >>>Margaret >>>Sent: Wed 5/9/2007 12:42 PM >>>To: histonet@lists.utsouthwestern.edu >>>Subject: [Histonet] timer calibration >>> >>> >>> >>>We calibrate our timers by doing the following. Use the telephone to >>>call 303-499-7111. A voice will prompt you and tell you the time. At >>>the minute turn on the timer and record the Coordinated Universal time >>>and the timer time. Listen until the next minute and turn off the timer >>>and record the Coordinated Universal Time and the time on the timer. >>> >>> >>> >>> >>> >>>Margaret Perry HT (ASCP) >>> >>>IHC Lab Manager Veterinary Science >>> >>>Animal Disease Research and Diagnostic Lab >>> >>>South Dakota State University >>> >>>Box 2175 North Campus Drive >>> >>>Brookings SD 57007 >>> >>> >>> >>>_______________________________________________ >>>Histonet mailing list >>>Histonet@lists.utsouthwestern.edu >>>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >>> >>>_______________________________________________ >>>Histonet mailing list >>>Histonet@lists.utsouthwestern.edu >>>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Best Regards, > > Pamela A Marcum > Manager, Histology Special Procedures > University of Pennsylvania > School of Veterinary Medicine > R.S. Reynolds Jr. CORL > New Bolton Center > 382 West Street Road > Kennett Square, PA 19348 > > Phone - 610-925-6278 > Fax - 610-925-8120 > E-mail - pmarcum@vet.upenn.edu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From kgrobert <@t> rci.rutgers.edu Wed May 9 15:38:12 2007 From: kgrobert <@t> rci.rutgers.edu (Kathleen Roberts) Date: Wed May 9 15:33:03 2007 Subject: [Histonet] Interesting discussion about RE: timer calibration In-Reply-To: References: <08A0A863637F1349BBFD83A96B27A50A12003E@uwhis-xchng3.uwhis.hosp.wisc.edu> <6.0.0.22.1.20070509132552.01b40ae8@gemini.msu.montana.edu> Message-ID: <46423134.2070602@rci.rutgers.edu> God forbid that you & your pathologist see the same color differently...I go through this with my boss every once in a while. An H&E slide will look just fine to me, but to him it's too pink, whereupon he tells me to go clean my glasses, change out the H&E and restain the slide. :oP Fortunately, our lab is a university research lab, so we don't have to undergo CAP inspection. -Kathy Neurotoxicology Labs Dept of Pharmacology & Toxicology Rutgers University Philip Oshel wrote: > Sure, why not? Require all histotechs to pass a "color-blindness" > (note the quotes) eye exam. Given that women generally have better > color discrimination than men in the red-green end of the spectrum, > female histotechs should be more valuble and able to demand higher pay. > I can just see medical supply houses all carrying Pantone color charts > ... > > Phil > >> I have followed this discussion with interest and a bit of humor on >> the rationale for this chore. It escapes me a bit - but I did like >> the idea of an atomic clock but then saw the reply about battery >> failure. Also, Greenwich time seemed the most logical over-all, >> always available and correct. I was most bothered by a second added >> onto every year, and that means I get older by the second annually. >> Hmmmm - but then the need for calibrated timers for validation >> purposes with complex testing? Is it the automated >> stainers/processors they are worried about? >> >> As for resetting timers to do staining, my eyes are the timer when >> developing a chromogen during manual IHC procedures - this is >> controlled with a microscope. I have let a clock run up in time to >> garner a "ballpark" figure for optimal development, and found that >> can vary from day to day too, not an exact timing to be sure. >> >> I agree with Claire on silver staining, but depend on microscopic >> examination to monitor silver (for finalizing GMS, Jones methenamine >> silver) ,and other proper removal of dyes Luxol fast blue, >> decolorizing tissue Gram stain. >> >> Now for a question: Do you think CAP will ever require eye >> calibration? Sorry to even suggest that - >> >> Gayle Callis HTL, HT, MT(ASCP) >> Research Histopathology Supervisor >> Veterinary Molecular Biology >> Montana State University >> Bozeman MT 59717 >> >> >> At 01:02 PM 5/9/2007, you wrote: >> >>> >>> Why don't we all just get atomic clocks and be done with it. I don't >>> believe they ever need to be calibrated. (unless the laws of physics >>> and radioactive element half-lives suddenly change). I agree with >>> Joe. Staining is a special talent anyway. I have had to reset timers >>> to add more incubation time on stains lots of times (especially >>> silver). Oh sorry, it's only Wednesday. Only two more days to go. >>> >>> Claire Ingles >>> UW Hospital >>> Madison WI >>> >>> ________________________________ >>> >>> From: histonet-bounces@lists.utsouthwestern.edu on behalf of Perry, >>> Margaret >>> Sent: Wed 5/9/2007 12:42 PM >>> To: histonet@lists.utsouthwestern.edu >>> Subject: [Histonet] timer calibration >>> >>> >>> >>> We calibrate our timers by doing the following. Use the telephone to >>> call 303-499-7111. A voice will prompt you and tell you the time. At >>> the minute turn on the timer and record the Coordinated Universal time >>> and the timer time. Listen until the next minute and turn off the >>> timer >>> and record the Coordinated Universal Time and the time on the timer. >>> >>> >>> >>> >>> >>> Margaret Perry HT (ASCP) >>> >>> IHC Lab Manager Veterinary Science >>> >>> Animal Disease Research and Diagnostic Lab >>> >>> South Dakota State University >>> >>> Box 2175 North Campus Drive >>> >>> Brookings SD 57007 >> > From Janet.Bonner <@t> flhosp.org Wed May 9 15:32:18 2007 From: Janet.Bonner <@t> flhosp.org (Bonner, Janet) Date: Wed May 9 15:33:20 2007 Subject: [Histonet] Interesting discussion about RE: timer calibration References: <08A0A863637F1349BBFD83A96B27A50A12003E@uwhis-xchng3.uwhis.hosp.wisc.edu><6.0.0.22.1.20070509132552.01b40ae8@gemini.msu.montana.edu> Message-ID: <5F31F38C96781A4FBE3196EBC22D47802E0FA7@fhosxchmb006.ADVENTISTCORP.NET> Actually, we do get the color-blindness test here. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Philip Oshel Sent: Wed 5/9/2007 4:23 PM To: Histonet@Pathology.swmed.edu Subject: Re: [Histonet] Interesting discussion about RE: timer calibration Sure, why not? Require all histotechs to pass a "color-blindness" (note the quotes) eye exam. Given that women generally have better color discrimination than men in the red-green end of the spectrum, female histotechs should be more valuble and able to demand higher pay. I can just see medical supply houses all carrying Pantone color charts ... Phil >I have followed this discussion with interest and a bit of humor on >the rationale for this chore. It escapes me a bit - but I did like >the idea of an atomic clock but then saw the reply about battery >failure. Also, Greenwich time seemed the most logical over-all, >always available and correct. I was most bothered by a second added >onto every year, and that means I get older by the second annually. >Hmmmm - but then the need for calibrated timers for validation >purposes with complex testing? Is it the automated >stainers/processors they are worried about? > >As for resetting timers to do staining, my eyes are the timer when >developing a chromogen during manual IHC procedures - this is >controlled with a microscope. I have let a clock run up in time to >garner a "ballpark" figure for optimal development, and found that >can vary from day to day too, not an exact timing to be sure. > >I agree with Claire on silver staining, but depend on microscopic >examination to monitor silver (for finalizing GMS, Jones methenamine >silver) ,and other proper removal of dyes Luxol fast blue, >decolorizing tissue Gram stain. > >Now for a question: Do you think CAP will ever require eye >calibration? Sorry to even suggest that - > >Gayle Callis HTL, HT, MT(ASCP) >Research Histopathology Supervisor >Veterinary Molecular Biology >Montana State University >Bozeman MT 59717 > > > At 01:02 PM 5/9/2007, you wrote: >> >>Why don't we all just get atomic clocks and be done with it. I >>don't believe they ever need to be calibrated. (unless the laws of >>physics and radioactive element half-lives suddenly change). I >>agree with Joe. Staining is a special talent anyway. I have had to >>reset timers to add more incubation time on stains lots of times >>(especially silver). Oh sorry, it's only Wednesday. Only two more >>days to go. >> >>Claire Ingles >> UW Hospital >>Madison WI >> >>________________________________ >> >>From: histonet-bounces@lists.utsouthwestern.edu on behalf of Perry, Margaret >>Sent: Wed 5/9/2007 12:42 PM >>To: histonet@lists.utsouthwestern.edu >>Subject: [Histonet] timer calibration >> >> >> >>We calibrate our timers by doing the following. Use the telephone to >>call 303-499-7111. A voice will prompt you and tell you the time. At >>the minute turn on the timer and record the Coordinated Universal time >>and the timer time. Listen until the next minute and turn off the timer >>and record the Coordinated Universal Time and the time on the timer. >> >> >> >> >> >>Margaret Perry HT (ASCP) >> >>IHC Lab Manager Veterinary Science >> >>Animal Disease Research and Diagnostic Lab >> >>South Dakota State University >> >>Box 2175 North Campus Drive >> >>Brookings SD 57007 -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 voice: (989) 774-3576 dept. fax: (989) 774-3462 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From Barry.R.Rittman <@t> uth.tmc.edu Wed May 9 15:37:14 2007 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Wed May 9 15:37:19 2007 Subject: [Histonet] Topic of interest Message-ID: Might I respectfully request that you use the subject line for the topic, a lot of the email have this line blank and so dam not sure if they are the same topic or different ones. It is difficult for a person that has only had one cup of coffee today and a heavy lunch to have to open up and delete all these emails. I appreciate (or will) your help with this. Thank you Barry From JWEEMS <@t> sjha.org Wed May 9 15:38:05 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed May 9 15:38:32 2007 Subject: [Histonet] Interesting discussion about RE: timer calibration In-Reply-To: Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FF97D0@sjhaexc02.sjha.org> We already do the color blind thing, how do you not! It's in there somewhere. Doesn't it make you want to quit doing CAP? You all are 2 fun!!! and it's only Wednesday... Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Philip Oshel Sent: Wednesday, May 09, 2007 4:23 PM To: Histonet@Pathology.swmed.edu Subject: Re: [Histonet] Interesting discussion about RE: timer calibration Sure, why not? Require all histotechs to pass a "color-blindness" (note the quotes) eye exam. Given that women generally have better color discrimination than men in the red-green end of the spectrum, female histotechs should be more valuble and able to demand higher pay. I can just see medical supply houses all carrying Pantone color charts ... Phil >I have followed this discussion with interest and a bit of humor on >the rationale for this chore. It escapes me a bit - but I did like >the idea of an atomic clock but then saw the reply about battery >failure. Also, Greenwich time seemed the most logical over-all, >always available and correct. I was most bothered by a second added >onto every year, and that means I get older by the second annually. >Hmmmm - but then the need for calibrated timers for validation >purposes with complex testing? Is it the automated >stainers/processors they are worried about? > >As for resetting timers to do staining, my eyes are the timer when >developing a chromogen during manual IHC procedures - this is >controlled with a microscope. I have let a clock run up in time to >garner a "ballpark" figure for optimal development, and found that >can vary from day to day too, not an exact timing to be sure. > >I agree with Claire on silver staining, but depend on microscopic >examination to monitor silver (for finalizing GMS, Jones methenamine >silver) ,and other proper removal of dyes Luxol fast blue, >decolorizing tissue Gram stain. > >Now for a question: Do you think CAP will ever require eye >calibration? Sorry to even suggest that - > >Gayle Callis HTL, HT, MT(ASCP) >Research Histopathology Supervisor >Veterinary Molecular Biology >Montana State University >Bozeman MT 59717 > > > At 01:02 PM 5/9/2007, you wrote: >> >>Why don't we all just get atomic clocks and be done with it. I >>don't believe they ever need to be calibrated. (unless the laws of >>physics and radioactive element half-lives suddenly change). I >>agree with Joe. Staining is a special talent anyway. I have had to >>reset timers to add more incubation time on stains lots of times >>(especially silver). Oh sorry, it's only Wednesday. Only two more >>days to go. >> >>Claire Ingles >> UW Hospital >>Madison WI >> >>________________________________ >> >>From: histonet-bounces@lists.utsouthwestern.edu on behalf of Perry, Margaret >>Sent: Wed 5/9/2007 12:42 PM >>To: histonet@lists.utsouthwestern.edu >>Subject: [Histonet] timer calibration >> >> >> >>We calibrate our timers by doing the following. Use the telephone to >>call 303-499-7111. A voice will prompt you and tell you the time. At >>the minute turn on the timer and record the Coordinated Universal time >>and the timer time. Listen until the next minute and turn off the timer >>and record the Coordinated Universal Time and the time on the timer. >> >> >> >> >> >>Margaret Perry HT (ASCP) >> >>IHC Lab Manager Veterinary Science >> >>Animal Disease Research and Diagnostic Lab >> >>South Dakota State University >> >>Box 2175 North Campus Drive >> >>Brookings SD 57007 -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 voice: (989) 774-3576 dept. fax: (989) 774-3462 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From trinimaican2501 <@t> yahoo.com Wed May 9 15:40:17 2007 From: trinimaican2501 <@t> yahoo.com (I-sanna Gibbons) Date: Wed May 9 15:40:20 2007 Subject: [Histonet] brain histology Message-ID: <581897.86999.qm@web50305.mail.re2.yahoo.com> I am having a bit of trouble obtaining good quality slides of bat brains. The brains are fixed in Carnoy's, stored in absolute alcohol, processed routinely, infiltrated with paraffin, sectioned at 8 microns and stained using cresyl fast violet. The aim is to record the cytoarchitecture. The resulting slides show poor tissue integrity with extensive cracking. Can anyone suggest how this can be avoided? I was thinking maybe a double-embedding method for whole brains (10-15 mm) and thicker sections (25-30 microns)? Thanking you in advance I-sanna Gibbons, DVM Veterinary Anatomy School of Veterinary Medicine The University of the West Indies Trinidad and Tobago, W.I. --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From trinimaican2501 <@t> yahoo.com Wed May 9 15:48:01 2007 From: trinimaican2501 <@t> yahoo.com (I-sanna Gibbons) Date: Wed May 9 15:48:06 2007 Subject: [Histonet] images of bat midbrain Message-ID: <296805.65426.qm@web50304.mail.re2.yahoo.com> can anyone send me some images of what normal bat brain (midbrain especially) histology looks like? Thanks I-sanna Gibbons DVM Veterinary Anatomy School of Veterinary Medicine The University of the West Indies Trinidad and Tobago, W.I. --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From rjbuesa <@t> yahoo.com Wed May 9 15:50:30 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 9 15:50:34 2007 Subject: [Histonet] brain histology In-Reply-To: <581897.86999.qm@web50305.mail.re2.yahoo.com> Message-ID: <741106.41997.qm@web61225.mail.yahoo.com> I think is too much time in alcohol(s). Storing in absolute EthOL is not a good practice. Try to reduce this aspect and check for results. Ren? J. I-sanna Gibbons wrote: I am having a bit of trouble obtaining good quality slides of bat brains. The brains are fixed in Carnoy's, stored in absolute alcohol, processed routinely, infiltrated with paraffin, sectioned at 8 microns and stained using cresyl fast violet. The aim is to record the cytoarchitecture. The resulting slides show poor tissue integrity with extensive cracking. Can anyone suggest how this can be avoided? I was thinking maybe a double-embedding method for whole brains (10-15 mm) and thicker sections (25-30 microns)? Thanking you in advance I-sanna Gibbons, DVM Veterinary Anatomy School of Veterinary Medicine The University of the West Indies Trinidad and Tobago, W.I. --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From alaskagirl1950 <@t> yahoo.com Wed May 9 16:06:18 2007 From: alaskagirl1950 <@t> yahoo.com (Patricia Adams) Date: Wed May 9 16:06:25 2007 Subject: [Histonet]Coffee? Topic of interest In-Reply-To: Message-ID: <20070509210618.45286.qmail@web52506.mail.re2.yahoo.com> Bummer! I have been maybe on the up-side of coffee today, might be where the rather small nagging headache came from. Or maybe the continuous group of DVM's and PhD's that keep trouping in to ask which antibodies I have on hand and will they work on "pick your species". And the phone has not stopped ringing today! Or maybe I need a little more coffee, or maybe its that missing second for this year......bummer. Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From ccross6032 <@t> aol.com Wed May 9 16:10:43 2007 From: ccross6032 <@t> aol.com (Cheryl Cross) Date: Wed May 9 16:11:03 2007 Subject: [Histonet] brain histology In-Reply-To: <741106.41997.qm@web61225.mail.yahoo.com> References: <741106.41997.qm@web61225.mail.yahoo.com> Message-ID: <92ADA3E1-F789-4143-AA9E-031B798E4BE2@aol.com> Hi Dr. Gibbons - Great to see a fellow vet posting!! I do some neurotox stuff in mice. What makes for a really nice FFPE section is, if you can, perfuse fixing the rodents, then removing the calvarium overlying the brain, and letting the brain sit in the skull in your fixative of choice (I use 10% NBF). Letting the brain sit for 24 hours in the fix allows you to avoid those dreaded dark angular neurons. After sitting for 24 hours I take out the brain and section routinely ... if I can't section immediately and immunohistochemistry will be performed i switch it to 70% EtOH ... but sitting in the alcohol makes the tissue very very firm. If immunohistochemistry isn't an issue I allow the brain or organs to remain in the 10% NBF. Hope this helps, let me know if you need a protocol for perfusion, etc. Cheryl Cheryl Cross, DVM, Dipl. ACVP Researcher University Corporation for Atmospheric Research College of Veterinary Medicine University of Tennessee Department of Pathology 2407 River Drive, Room A201 Knoxville, TN 37996-4542 (423) 967-2724 fax: 865-974-5616 ccross@ucar.edu On May 9, 2007, at 4:50 PM, Rene J Buesa wrote: > I think is too much time in alcohol(s). Storing in absolute EthOL > is not a good practice. > Try to reduce this aspect and check for results. > Ren? J. > > I-sanna Gibbons wrote: > I am having a bit of trouble obtaining good quality slides of bat > brains. The brains are fixed in Carnoy's, stored in absolute > alcohol, processed routinely, infiltrated with paraffin, sectioned > at 8 microns and stained using cresyl fast violet. The aim is to > record the cytoarchitecture. The resulting slides show poor tissue > integrity with extensive cracking. Can anyone suggest how this can > be avoided? I was thinking maybe a double-embedding method for > whole brains (10-15 mm) and thicker sections (25-30 microns)? > > Thanking you in advance > > I-sanna Gibbons, DVM > Veterinary Anatomy > School of Veterinary Medicine > The University of the West Indies > Trinidad and Tobago, W.I. > > > --------------------------------- > Ahhh...imagining that irresistible "new car" smell? > Check outnew cars at Yahoo! Autos. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Ahhh...imagining that irresistible "new car" smell? > Check outnew cars at Yahoo! Autos. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ccross6032 <@t> aol.com Wed May 9 16:15:35 2007 From: ccross6032 <@t> aol.com (Cheryl Cross) Date: Wed May 9 16:15:45 2007 Subject: [Histonet] images of bat midbrain In-Reply-To: <296805.65426.qm@web50304.mail.re2.yahoo.com> References: <296805.65426.qm@web50304.mail.re2.yahoo.com> Message-ID: <30A72922-9264-4C43-A7D9-F3C205173262@aol.com> Try this online atlas, It's really nice for normal structures (especially if you are using Cresyl Echt Violet stain) - this is a mouse atlas but hopefully will help. Also you can chose transverse or sagittal sections: http://www.brain-map.org/welcome.do On the right side of the page click on "launch the Allen reference Atlas" and then you can choose the coordinates that best match your sections. Cheryl Cheryl A. Cross, DVM, Dipl. ACVP Researcher University Corporation for Atmospheric Research College of Veterinary Medicine University of Tennessee Department of Pathology 2407 River Drive, Room A201 Knoxville, TN 37996-4542 (423) 967-2724 fax: 865-974-5616 ccross@ucar.edu On May 9, 2007, at 4:48 PM, I-sanna Gibbons wrote: > can anyone send me some images of what normal bat brain (midbrain > especially) histology looks like? > Thanks > > I-sanna Gibbons DVM > Veterinary Anatomy > School of Veterinary Medicine > The University of the West Indies > Trinidad and Tobago, W.I. > > > --------------------------------- > Ahhh...imagining that irresistible "new car" smell? > Check outnew cars at Yahoo! Autos. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From soofias2 <@t> yahoo.com Wed May 9 16:17:16 2007 From: soofias2 <@t> yahoo.com (soofia siddiqui) Date: Wed May 9 16:17:18 2007 Subject: [Histonet] Interesting discussion about RE: timer calibration In-Reply-To: <46423134.2070602@rci.rutgers.edu> Message-ID: <170534.73285.qm@web39515.mail.mud.yahoo.com> Is not the peer review of the slides to confirm the pathologists diagnosis is a kind of calibration of a pathologists eyes, knowledge, and understanding? Peer review is required after every six month. I request the other pathologist to check the slides to check on my boss' diagnosis.I wished my eyes could be calibrated with my teachers' eyes when I was attending hematology class. Soofia UW Hospital & Clinics Madison WI --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From gcallis <@t> montana.edu Wed May 9 16:19:02 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed May 9 16:19:12 2007 Subject: website for brains Re: [Histonet] brain histology In-Reply-To: <741106.41997.qm@web61225.mail.yahoo.com> References: <581897.86999.qm@web50305.mail.re2.yahoo.com> <741106.41997.qm@web61225.mail.yahoo.com> Message-ID: <6.0.0.22.1.20070509151733.01b7ee00@gemini.msu.montana.edu> Try this website - I am not sure they have bat, but they certainly have many other species - it is a delight to look at. www.brainmaps.com Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 From Tony_Reilly <@t> health.qld.gov.au Wed May 9 17:27:29 2007 From: Tony_Reilly <@t> health.qld.gov.au (Anthony Reilly) Date: Wed May 9 17:28:04 2007 Subject: [Histonet] timer calibration Message-ID: Hi I agree with Joe that histology timers do not need to be calibrated, but they are apparently in other scientific disciplines. A simple and quick method which does not require any special equipment is to test your timers over a stipulated time frame against the time supplied service by your telephone provider. The time is usually expressed in 10 second intervals. If your timer complied over a one minute period it would be deemed satisfactory. regards Tony Reilly Chief Scientist Anatomical Pathology QHPS-Prince Charles Hospital Rode Rd Chermside Q 4032 Australia Ph: 07 3139 4543 Fax: 07 3193 4546 tony_reilly@health.qld.gov.au >>> "Joe Nocito" 05/15/07 1:08 am >>> I'm sorry am I the only one that thinks calibrating timers is stupid. I mean how many histology procedures are so time sensitive that the timers have to be calibrated? Let's face it. I have had techs sit there and watch the clock and rinse as soon as the timer goes off. And I've had techs wait for the timer to go off , then mosey over and rinse. Both stains worked. I guess I'm just not anal enough. JTT ----- Original Message ----- From: "Rene J Buesa" To: "Margiotta, Michele" ; Sent: Tuesday, May 08, 2007 3:39 PM Subject: Re: [Histonet] timer calibration > Try any local watch repair store. They have to calibrate their repaired > watches so it is very likely they can help you. > Ren? J. > > "Margiotta, Michele" wrote: > Hi All, > > Does anyone have a procedure for calibrating timers? We just had an > inspection and got cited because our timers were not calibrated. Any info > would be appreciated! > > Michele > > > > This e-mail and any files transmitted with it are confidential and are > intended > solely for the use of the individual or entity to which they are > addressed. > This communication may contain material protected by the attorney-client > privilege. If you are not the intended recipient or the person responsible > for > delivering the e-mail to the intended recipient, be advised that you have > received > this e-mail in error and that any use, dissemination, forwarding, > printing, > or copying of this e-mail is strictly prohibited. If you have received > this e-mail in > error, please immediately notify the sender via return e-mail or call > Brookhaven Memorial Hospital Medical Center at (631) 654-7282. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Ahhh...imagining that irresistible "new car" smell? > Check outnew cars at Yahoo! Autos. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/ received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. **************************************************************** From caramel-bonbon <@t> caramail.com Thu May 10 00:03:29 2007 From: caramel-bonbon <@t> caramail.com (Hired Blade) Date: Thu May 10 00:03:36 2007 Subject: [Histonet] Proof of Employment for HT Cert-Testing? Message-ID: <183536364416634@lycos-europe.com> Hello, I am wondering if anybody who has recently taken the c exams for HT(ASCP) via the "year-of-employment" (plus acedmi requirements) route can tell me how the ASCP asks for proof of ones yea Path-MD, or perhaps copies of starting and ending pay-check statements?& gear up for ju official ASCP Thanks. [images.=] M?me le plus noble des super-h?ros poss?de sa part d'ombre... Lib?rez-la! From laurie.reilly <@t> jcu.edu.au Thu May 10 02:55:51 2007 From: laurie.reilly <@t> jcu.edu.au (Laurie Reilly) Date: Thu May 10 02:56:03 2007 Subject: [Histonet] timer calibration In-Reply-To: <1F937FB30BDB7C4A9F39F83FEA8D379F07EE44@bruexchange1.digestivespecialists.com> References: <08A0A863637F1349BBFD83A96B27A50A12003E@uwhis-xchng3.uwhis.hosp.wisc.edu> <1F937FB30BDB7C4A9F39F83FEA8D379F07EE44@bruexchange1.digestivespecialists.com> Message-ID: <002001c792d8$a1dc9e90$de55db89@health.ad.jcu.edu.au> The timer in your head probably doesn't need calibrating. How often do we set a timer for a stain that we do regularly and then get busy with something else? All of a sudden we think "that timer should have gone off" and when we take it from our pocket there's 2 seconds to go. Regards, Laurie. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Thursday, 10 May 2007 5:11 AM To: Ingles Claire; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] timer calibration I can't figure out how to calibrate the timer that lives in my head. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Wednesday, May 09, 2007 3:02 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] timer calibration Why don't we all just get atomic clocks and be done with it. I don't believe they ever need to be calibrated. (unless the laws of physics and radioactive element half-lives suddenly change). I agree with Joe. Staining is a special talent anyway. I have had to reset timers to add more incubation time on stains lots of times (especially silver). Oh sorry, it's only Wednesday. Only two more days to go. Claire Ingles UW Hospital Madison WI ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Perry, Margaret Sent: Wed 5/9/2007 12:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] timer calibration We calibrate our timers by doing the following. Use the telephone to call 303-499-7111. A voice will prompt you and tell you the time. At the minute turn on the timer and record the Coordinated Universal time and the timer time. Listen until the next minute and turn off the timer and record the Coordinated Universal Time and the time on the timer. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Thu May 10 06:06:54 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Thu May 10 06:07:01 2007 Subject: [Histonet] Ki 67 Message-ID: My supply of antibody to Ki67 is soon to run out, can anyone let me know which antibody is currently the one of their choice when working with formalin fixed paraffin processed rodent tissues??, thanks. Richard Edwards MRC TOXICOLOGY UNIT U.K. From JMacDonald <@t> mtsac.edu Thu May 10 07:15:17 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu May 10 07:15:31 2007 Subject: [Histonet] Proof of Employment for HT Cert-Testing? In-Reply-To: <183536364416634@lycos-europe.com> Message-ID: The application form for applying for the certification has an area for filling out the employment information area. You can down load the application at http://www.ascp.org/Certification/CertifyingExaminations/cert_procedures/ Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu "Hired Blade" Sent by: histonet-bounces@lists.utsouthwestern.edu 05/09/2007 10:03 PM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Proof of Employment for HT Cert-Testing? Hello, I am wondering if anybody who has recently taken the c= ertification exams for HT(ASCP) via the "year-of-employment" (plus acedmi= c requirements) route can tell me how the ASCP asks for proof of ones yea= r-term fulfilled? Is it a signed letter from your supervising Path-MD, or perhaps copies of starting and ending pay-check statements?&= nbsp; I am nearing my firstyear-mark and starting to gear up for ju= mping this hoop with some excitement! I looked on the official ASCP= site and couldn't seem to find this answer by the way. Thanks. [images.=] M?me le plus noble des super-h?ros poss?de sa part d'ombre... Lib?rez-la! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RitaR <@t> lexhealth.org Thu May 10 08:50:19 2007 From: RitaR <@t> lexhealth.org (Rita Riddle) Date: Thu May 10 08:50:26 2007 Subject: [Histonet] Salary - San Antonio Message-ID: I have a co-worker moving to San Antonio. She is certified, has 9 years of experience. Knows Special Stains and Immunohistochemistry. What can she expect as far as salary range in that area. Thanks Rita Rita Riddle HT(ASCP) Lead Histology Tech Lexington Medical Center West Columbia, SC 803-791-2881 _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. From dusko.trajkovic <@t> pfizer.com Thu May 10 09:00:33 2007 From: dusko.trajkovic <@t> pfizer.com (Trajkovic, Dusko) Date: Thu May 10 09:01:03 2007 Subject: [Histonet] Ki 67 In-Reply-To: Message-ID: <3AD0BD3142459B4E9B12CBEAFF2B89B2046A69D5@lajamrexm01.amer.pfizer.com> Lab Vision / Neomarkers - Rabbit monoclonal Cat# RM9106 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, R.E. Sent: Thursday, May 10, 2007 4:07 AM To: Histonet Subject: [Histonet] Ki 67 My supply of antibody to Ki67 is soon to run out, can anyone let me know which antibody is currently the one of their choice when working with formalin fixed paraffin processed rodent tissues??, thanks. Richard Edwards MRC TOXICOLOGY UNIT U.K. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- LEGAL NOTICE Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this E-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately. From TJJ <@t> Stowers-Institute.org Thu May 10 09:07:25 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Thu May 10 09:07:45 2007 Subject: [Histonet] Re: Ki67 (in rodents) Message-ID: Richard, we use Labvision's rabbit monoclonal Ki67 with great success in mouse. I highly recommend it. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From making <@t> ufl.edu Thu May 10 09:16:32 2007 From: making <@t> ufl.edu (MKing) Date: Thu May 10 09:09:42 2007 Subject: [Histonet] timer calibration Message-ID: <46432940.1010308@ufl.edu> If the regs don't specify what to calibrate to, it doesn't make any difference whether you use atomic clocks or a calendar, they're still calibrated. From sonya.martin <@t> soton.ac.uk Thu May 10 09:19:20 2007 From: sonya.martin <@t> soton.ac.uk (Martin S.) Date: Thu May 10 09:20:38 2007 Subject: [Histonet] Crumbling frozen sections Message-ID: <71437982F5B13A4D9A5B2669BDB89EE4023E3590@ISS-CL-EX-V1.soton.ac.uk> Hi All, I have been doing a lot of frozen sectioning of mouse tissues. I remove the organ (spleen, lymph nodes, liver etc) from the mouse immerse it in OCT in a foil mould and then place in a bath of isopentane on dry ice. This has been working really well and I have been getting good sections. I am now looking at some human tissue (colo-rectal and liver tumours). I receive a small piece of tissue from the surgeon which I have been treating as for the mouse tissue. However I am finding it very hard to get good sections. The tissue seems very flaky and crumbly and either disintegrates on cutting or if I do get what looks like an ok section by the time I have gone through the staining procedure it has completely gone! I am not sure how long it takes between the tissue being removed and me getting the sample - maybe 30min - could this be the cause? I have some old colo-rectal tumours that were snap frozen in liquid nitrogen and they seem much better - I think I'll try doing this from now on but just wondering if anyone has any insight into why I'm having such problems. Thanks! Sonya From carl.hobbs <@t> kcl.ac.uk Thu May 10 09:20:56 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Thu May 10 09:21:21 2007 Subject: [Histonet] re:Ki67 Message-ID: I regularly use Labvision's rabbit monoclonal ( SP6clone #RM-9106) and also BDPharmingen's mouse monoclonal ( B56 clone #556003) both are excellent , after HIER, on mouse and rat FFPW sections If you just need one: I personally would recommend the BD Pharma one. In my hands, not only is it more consistent but I can dilute it around 1/1000. Whereas I can only dilute SP6 to 1/100, for equally effective staining. Strangely enough, in our hands SP6 works much better than B56 for perfuse-fixed frozen sections. Carl Try looking here for some more info/pics http://www.immunoportal.com/index.php Histology Manager, Wolfson CARD Kings College London Guys Campus London SE1 1UL England From asachau <@t> titanmed.com Thu May 10 09:26:37 2007 From: asachau <@t> titanmed.com (April Sachau) Date: Thu May 10 09:27:34 2007 Subject: [Histonet] Ohio Position In-Reply-To: <71437982F5B13A4D9A5B2669BDB89EE4023E3590@ISS-CL-EX-V1.soton.ac.uk> Message-ID: <7E3ACD48BA6E26408F3188FBF08693F78D3B0B@titansbs1.corp.titanmed.com> Hi Histo-netters! I am working with a hospital in Ohio that is in need of an ASCP certified Histologist for a temporary contract. They tentatively need someone the first week of June. Day shift/40 hours a week. Please reply if you are interested or if you know someone interested. Thanks! From c.m.vanderloos <@t> amc.uva.nl Thu May 10 09:36:16 2007 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Thu May 10 09:36:43 2007 Subject: [Histonet] RE: Ki 67 Message-ID: Richard, Go for Ki67 rabbit monoclonal, clone SP6 (LabVision/Neomarkers). HIER with Tris-EDTA9.0 / 1:500 (60 min, RT) / PowerVision anti-Rb/HRP / DAB+ Cheers, Chris Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands phone: +31 20 5665631 Date: Thu, 10 May 2007 12:06:54 +0100 From: "Edwards, R.E." Subject: [Histonet] Ki 67 To: "Histonet" [1]histonet@lists.utsouthwestern.edu My supply of antibody to Ki67 is soon to run out, can anyone let me know which antibody is currently the one of their choice when working with formalin fixed paraffin processed rodent tissues??, thanks. Richard Edwards MRC TOXICOLOGY UNIT U.K. References 1. mailto:histonet@lists.utsouthwestern.edu From jnocito <@t> satx.rr.com Tue May 15 09:58:12 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu May 10 09:58:14 2007 Subject: [Histonet] timer calibration References: <113979.14134.qm@web61212.mail.yahoo.com> <00c801c79639$bc0a91a0$d49eae18@yourxhtr8hvc4p> <002d01c79259$22348380$6500a8c0@mainbox> Message-ID: <017a01c79701$75e23930$d49eae18@yourxhtr8hvc4p> They might, but you know me, I'm not going down without a fight!!! I need a name change. Instead of Joe "The Toe", How about Joe "The Timer?" I still get to keep my initials "JTT" Aaauggggg!! Is it Friday yet? JTT ----- Original Message ----- From: "Bryan Hewlett" To: "Joe Nocito" ; "Rene J Buesa" ; "Margiotta, Michele" ; Sent: Wednesday, May 09, 2007 11:43 AM Subject: Re: [Histonet] timer calibration > Joe, > > You are talking fundamental philosophical differences here. > The inspectors are obviously Newtonian realists, while you're leaning more > to the Leibnitz/Kant view. > They are going to win! > > Bryan > > ----- Original Message ----- > From: "Joe Nocito" > To: "Rene J Buesa" ; "Margiotta, Michele" > ; > Sent: Monday, May 14, 2007 11:08 AM > Subject: Re: [Histonet] timer calibration > > > I'm sorry > am I the only one that thinks calibrating timers is stupid. I mean how > many > histology procedures are so time sensitive that the timers have to be > calibrated? Let's face it. I have had techs sit there and watch the clock > and rinse as soon as the timer goes off. And I've had techs wait for the > timer to go off , then mosey over and rinse. Both stains worked. I guess > I'm just not anal enough. > > JTT > ----- Original Message ----- > From: "Rene J Buesa" > To: "Margiotta, Michele" ; > > Sent: Tuesday, May 08, 2007 3:39 PM > Subject: Re: [Histonet] timer calibration > > >> Try any local watch repair store. They have to calibrate their repaired >> watches so it is very likely they can help you. >> Ren? J. >> >> "Margiotta, Michele" wrote: >> Hi All, >> >> Does anyone have a procedure for calibrating timers? We just had an >> inspection and got cited because our timers were not calibrated. Any info >> would be appreciated! >> >> Michele >> >> >> >> This e-mail and any files transmitted with it are confidential and are >> intended >> solely for the use of the individual or entity to which they are >> addressed. >> This communication may contain material protected by the attorney-client >> privilege. If you are not the intended recipient or the person >> responsible for >> delivering the e-mail to the intended recipient, be advised that you have >> received >> this e-mail in error and that any use, dissemination, forwarding, >> printing, >> or copying of this e-mail is strictly prohibited. If you have received >> this e-mail in >> error, please immediately notify the sender via return e-mail or call >> Brookhaven Memorial Hospital Medical Center at (631) 654-7282. >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> >> --------------------------------- >> Ahhh...imagining that irresistible "new car" smell? >> Check outnew cars at Yahoo! Autos. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mcauliff <@t> umdnj.edu Thu May 10 09:59:04 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu May 10 09:59:48 2007 Subject: website for brains Re: [Histonet] brain histology In-Reply-To: <6.0.0.22.1.20070509151733.01b7ee00@gemini.msu.montana.edu> References: <581897.86999.qm@web50305.mail.re2.yahoo.com> <741106.41997.qm@web61225.mail.yahoo.com> <6.0.0.22.1.20070509151733.01b7ee00@gemini.msu.montana.edu> Message-ID: <46433338.9060802@umdnj.edu> When I try the site below all I get is a bunch of ads. Clicking on brain maps just gets more ads. Geoff Gayle Callis wrote: > Try this website - I am not sure they have bat, but they certainly > have many other species - it is a delight to look at. > > www.brainmaps.com > > Gayle Callis HTL, HT, MT(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University > Bozeman MT 59717 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From jnocito <@t> satx.rr.com Tue May 15 10:00:32 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu May 10 10:00:35 2007 Subject: [Histonet] timer calibration References: <1033394974-763687002@pathology.swmed.edu> <5F31F38C96781A4FBE3196EBC22D47802E0F9E@fhosxchmb006.ADVENTISTCORP.NET> Message-ID: <019201c79701$c98b4ea0$d49eae18@yourxhtr8hvc4p> RE: [Histonet] timer calibrationnow you're talking ----- Original Message ----- From: Bonner, Janet To: Douglas D Deltour ; Joe Nocito ; histonet@pathology.swmed.edu Sent: Wednesday, May 09, 2007 12:40 PM Subject: RE: [Histonet] timer calibration Calibrated Clock Satellites? ------------------------------------------------------------------------------ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Douglas D Deltour Sent: Wed 5/9/2007 12:25 PM To: 'Joe Nocito'; histonet@pathology.swmed.edu Subject: RE: [Histonet] timer calibration Who inspects the calibrating timers? What are the calibrating timers calibrated against? What are the calibrating timers of the calibrating timers calibrated against? Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Monday, May 14, 2007 10:09 AM To: Rene J Buesa; Margiotta, Michele; histonet@pathology.swmed.edu Subject: Re: [Histonet] timer calibration I'm sorry am I the only one that thinks calibrating timers is stupid. I mean how many histology procedures are so time sensitive that the timers have to be calibrated? Let's face it. I have had techs sit there and watch the clock and rinse as soon as the timer goes off. And I've had techs wait for the timer to go off , then mosey over and rinse. Both stains worked. I guess I'm just not anal enough. JTT ----- Original Message ----- From: "Rene J Buesa" To: "Margiotta, Michele" ; Sent: Tuesday, May 08, 2007 3:39 PM Subject: Re: [Histonet] timer calibration > Try any local watch repair store. They have to calibrate their repaired > watches so it is very likely they can help you. > Ren? J. > > "Margiotta, Michele" wrote: > Hi All, > > Does anyone have a procedure for calibrating timers? We just had an > inspection and got cited because our timers were not calibrated. Any info > would be appreciated! > > Michele > > > > This e-mail and any files transmitted with it are confidential and are > intended > solely for the use of the individual or entity to which they are > addressed. > This communication may contain material protected by the attorney-client > privilege. If you are not the intended recipient or the person responsible > for > delivering the e-mail to the intended recipient, be advised that you have > received > this e-mail in error and that any use, dissemination, forwarding, > printing, > or copying of this e-mail is strictly prohibited. If you have received > this e-mail in > error, please immediately notify the sender via return e-mail or call > Brookhaven Memorial Hospital Medical Center at (631) 654-7282. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Ahhh...imagining that irresistible "new car" smell? > Check outnew cars at Yahoo! Autos. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From jnocito <@t> satx.rr.com Tue May 15 10:02:47 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu May 10 10:02:49 2007 Subject: [Histonet] timer calibration References: <08A0A863637F1349BBFD83A96B27A50A12003E@uwhis-xchng3.uwhis.hosp.wisc.edu> <1F937FB30BDB7C4A9F39F83FEA8D379F07EE44@bruexchange1.digestivespecialists.com> Message-ID: <01a001c79702$1a02ac20$d49eae18@yourxhtr8hvc4p> Take one hammer, place in one hand, and pound the crap out of the nearest inspector JTT ----- Original Message ----- From: "Blazek, Linda" To: "Ingles Claire" ; Sent: Wednesday, May 09, 2007 2:11 PM Subject: RE: [Histonet] timer calibration I can't figure out how to calibrate the timer that lives in my head. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Wednesday, May 09, 2007 3:02 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] timer calibration Why don't we all just get atomic clocks and be done with it. I don't believe they ever need to be calibrated. (unless the laws of physics and radioactive element half-lives suddenly change). I agree with Joe. Staining is a special talent anyway. I have had to reset timers to add more incubation time on stains lots of times (especially silver). Oh sorry, it's only Wednesday. Only two more days to go. Claire Ingles UW Hospital Madison WI ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Perry, Margaret Sent: Wed 5/9/2007 12:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] timer calibration We calibrate our timers by doing the following. Use the telephone to call 303-499-7111. A voice will prompt you and tell you the time. At the minute turn on the timer and record the Coordinated Universal time and the timer time. Listen until the next minute and turn off the timer and record the Coordinated Universal Time and the time on the timer. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Thu May 10 10:04:20 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Thu May 10 10:04:49 2007 Subject: [Histonet] timer calibration In-Reply-To: <017a01c79701$75e23930$d49eae18@yourxhtr8hvc4p> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FF97E6@sjhaexc02.sjha.org> Joe "The Toe Timer" ....(NOT two timer) Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Joe Nocito Sent: Tuesday, May 15, 2007 10:58 AM To: Bryan Hewlett; Rene J Buesa; Margiotta, Michele; histonet@pathology.swmed.edu Subject: Re: [Histonet] timer calibration They might, but you know me, I'm not going down without a fight!!! I need a name change. Instead of Joe "The Toe", How about Joe "The Timer?" I still get to keep my initials "JTT" Aaauggggg!! Is it Friday yet? JTT ----- Original Message ----- From: "Bryan Hewlett" To: "Joe Nocito" ; "Rene J Buesa" ; "Margiotta, Michele" ; Sent: Wednesday, May 09, 2007 11:43 AM Subject: Re: [Histonet] timer calibration > Joe, > > You are talking fundamental philosophical differences here. > The inspectors are obviously Newtonian realists, while you're leaning more > to the Leibnitz/Kant view. > They are going to win! > > Bryan > > ----- Original Message ----- > From: "Joe Nocito" > To: "Rene J Buesa" ; "Margiotta, Michele" > ; > Sent: Monday, May 14, 2007 11:08 AM > Subject: Re: [Histonet] timer calibration > > > I'm sorry > am I the only one that thinks calibrating timers is stupid. I mean how > many > histology procedures are so time sensitive that the timers have to be > calibrated? Let's face it. I have had techs sit there and watch the clock > and rinse as soon as the timer goes off. And I've had techs wait for the > timer to go off , then mosey over and rinse. Both stains worked. I guess > I'm just not anal enough. > > JTT > ----- Original Message ----- > From: "Rene J Buesa" > To: "Margiotta, Michele" ; > > Sent: Tuesday, May 08, 2007 3:39 PM > Subject: Re: [Histonet] timer calibration > > >> Try any local watch repair store. They have to calibrate their repaired >> watches so it is very likely they can help you. >> Ren? J. >> >> "Margiotta, Michele" wrote: >> Hi All, >> >> Does anyone have a procedure for calibrating timers? We just had an >> inspection and got cited because our timers were not calibrated. Any info >> would be appreciated! >> >> Michele >> >> >> >> This e-mail and any files transmitted with it are confidential and are >> intended >> solely for the use of the individual or entity to which they are >> addressed. >> This communication may contain material protected by the attorney-client >> privilege. If you are not the intended recipient or the person >> responsible for >> delivering the e-mail to the intended recipient, be advised that you have >> received >> this e-mail in error and that any use, dissemination, forwarding, >> printing, >> or copying of this e-mail is strictly prohibited. If you have received >> this e-mail in >> error, please immediately notify the sender via return e-mail or call >> Brookhaven Memorial Hospital Medical Center at (631) 654-7282. >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> >> --------------------------------- >> Ahhh...imagining that irresistible "new car" smell? >> Check outnew cars at Yahoo! Autos. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From jnocito <@t> satx.rr.com Tue May 15 10:05:37 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu May 10 10:05:43 2007 Subject: [Histonet] timer calibration References: <08A0A863637F1349BBFD83A96B27A50A12003E@uwhis-xchng3.uwhis.hosp.wisc.edu> <1F937FB30BDB7C4A9F39F83FEA8D379F07EE44@bruexchange1.digestivespecialists.com> <002001c792d8$a1dc9e90$de55db89@health.ad.jcu.edu.au> Message-ID: <01b901c79702$802d4e10$d49eae18@yourxhtr8hvc4p> I have the uncanny way of starting trouble, don't I? I hope the clock police are not monitoring my emails. I might get banned again. JTT ----- Original Message ----- From: "Laurie Reilly" To: "'Blazek, Linda'" ; "'Ingles Claire'" ; Sent: Thursday, May 10, 2007 2:55 AM Subject: RE: [Histonet] timer calibration > The timer in your head probably doesn't need calibrating. > How often do we set a timer for a stain that we do regularly and then get > busy with something else? All of a sudden we think "that timer should have > gone off" and when we take it from our pocket there's 2 seconds to go. > > Regards, Laurie. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, > Linda > Sent: Thursday, 10 May 2007 5:11 AM > To: Ingles Claire; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] timer calibration > > I can't figure out how to calibrate the timer that lives in my head. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles > Claire > Sent: Wednesday, May 09, 2007 3:02 PM > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] timer calibration > > > Why don't we all just get atomic clocks and be done with it. I don't > believe they ever need to be calibrated. (unless the laws of physics and > radioactive element half-lives suddenly change). I agree with Joe. > Staining is a special talent anyway. I have had to reset timers to add > more incubation time on stains lots of times (especially silver). Oh > sorry, it's only Wednesday. Only two more days to go. > > Claire Ingles > UW Hospital > Madison WI > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Perry, > Margaret > Sent: Wed 5/9/2007 12:42 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] timer calibration > > > > We calibrate our timers by doing the following. Use the telephone to > call 303-499-7111. A voice will prompt you and tell you the time. At > the minute turn on the timer and record the Coordinated Universal time > and the timer time. Listen until the next minute and turn off the timer > and record the Coordinated Universal Time and the time on the timer. > > > > > > Margaret Perry HT (ASCP) > > IHC Lab Manager Veterinary Science > > Animal Disease Research and Diagnostic Lab > > South Dakota State University > > Box 2175 North Campus Drive > > Brookings SD 57007 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Thu May 10 10:08:25 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu May 10 10:08:59 2007 Subject: [Histonet] brain histology In-Reply-To: <581897.86999.qm@web50305.mail.re2.yahoo.com> References: <581897.86999.qm@web50305.mail.re2.yahoo.com> Message-ID: <46433569.60706@umdnj.edu> Without fixation by perfusion the interior architecture will not be worth looking at. Why are you using Carnoy? Yes, it gives quick penetration but not qucik enough to get all the way through to the interior unless you slice the brain. Perfuse with buffered formalin, slice into 5-10 mm sections and fix for several days to a week, formalin works slowly. Then process for paraffin. As Rene stated, forget storage in abs. alcohol. Ten micron sections is fine for CNS. Also, there is a lot of work done on bat brain in the literature. Geoff I-sanna Gibbons wrote: >I am having a bit of trouble obtaining good quality slides of bat brains. The brains are fixed in Carnoy's, stored in absolute alcohol, processed routinely, infiltrated with paraffin, sectioned at 8 microns and stained using cresyl fast violet. The aim is to record the cytoarchitecture. The resulting slides show poor tissue integrity with extensive cracking. Can anyone suggest how this can be avoided? I was thinking maybe a double-embedding method for whole brains (10-15 mm) and thicker sections (25-30 microns)? > > Thanking you in advance > > I-sanna Gibbons, DVM > Veterinary Anatomy > School of Veterinary Medicine > The University of the West Indies > Trinidad and Tobago, W.I. > > >--------------------------------- >Ahhh...imagining that irresistible "new car" smell? > Check outnew cars at Yahoo! Autos. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From ree3 <@t> leicester.ac.uk Thu May 10 10:10:34 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Thu May 10 10:10:58 2007 Subject: [Histonet] timer calibration In-Reply-To: <01b901c79702$802d4e10$d49eae18@yourxhtr8hvc4p> References: <08A0A863637F1349BBFD83A96B27A50A12003E@uwhis-xchng3.uwhis.hosp.wisc.edu><1F937FB30BDB7C4A9F39F83FEA8D379F07EE44@bruexchange1.digestivespecialists.com><002001c792d8$a1dc9e90$de55db89@health.ad.jcu.edu.au> <01b901c79702$802d4e10$d49eae18@yourxhtr8hvc4p> Message-ID: They are, and you are!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: 15 May 2007 16:06 To: Laurie Reilly; 'Blazek, Linda'; 'Ingles Claire'; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] timer calibration I have the uncanny way of starting trouble, don't I? I hope the clock police are not monitoring my emails. I might get banned again. JTT ----- Original Message ----- From: "Laurie Reilly" To: "'Blazek, Linda'" ; "'Ingles Claire'" ; Sent: Thursday, May 10, 2007 2:55 AM Subject: RE: [Histonet] timer calibration > The timer in your head probably doesn't need calibrating. > How often do we set a timer for a stain that we do regularly and then get > busy with something else? All of a sudden we think "that timer should have > gone off" and when we take it from our pocket there's 2 seconds to go. > > Regards, Laurie. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, > Linda > Sent: Thursday, 10 May 2007 5:11 AM > To: Ingles Claire; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] timer calibration > > I can't figure out how to calibrate the timer that lives in my head. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles > Claire > Sent: Wednesday, May 09, 2007 3:02 PM > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] timer calibration > > > Why don't we all just get atomic clocks and be done with it. I don't > believe they ever need to be calibrated. (unless the laws of physics and > radioactive element half-lives suddenly change). I agree with Joe. > Staining is a special talent anyway. I have had to reset timers to add > more incubation time on stains lots of times (especially silver). Oh > sorry, it's only Wednesday. Only two more days to go. > > Claire Ingles > UW Hospital > Madison WI > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Perry, > Margaret > Sent: Wed 5/9/2007 12:42 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] timer calibration > > > > We calibrate our timers by doing the following. Use the telephone to > call 303-499-7111. A voice will prompt you and tell you the time. At > the minute turn on the timer and record the Coordinated Universal time > and the timer time. Listen until the next minute and turn off the timer > and record the Coordinated Universal Time and the time on the timer. > > > > > > Margaret Perry HT (ASCP) > > IHC Lab Manager Veterinary Science > > Animal Disease Research and Diagnostic Lab > > South Dakota State University > > Box 2175 North Campus Drive > > Brookings SD 57007 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MadaryJ <@t> MedImmune.com Thu May 10 10:11:01 2007 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Thu May 10 10:11:17 2007 Subject: [Histonet] Trying to find a train histo intern a job in Annapolis Md Message-ID: <8F3E1865E343C943BB38506D56FF01519C6C20@MD1EV002.medimmune.com> A college grad has been training in my lab(R&D in big pharma) for 3 months, but training has been intense. She is moving to the Annapolis area, and I was wondering if anyone knew of histolabs in that area? Respectfully, Nick Madary, HT/HTL(ASCP)QIHC Histology Lab Mgr I, Medimmune Inc One Medimmune Way Gaiithersburg, MD 20878 301.398.6113(lab) 301.398.4745(vm) 301.398.9745(fax) From ree3 <@t> leicester.ac.uk Thu May 10 10:17:52 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Thu May 10 10:18:00 2007 Subject: website for brains Re: [Histonet] brain histology In-Reply-To: <46433338.9060802@umdnj.edu> References: <6.0.0.22.1.20070509151733.01b7ee00@gemini.msu.montana.edu> <46433338.9060802@umdnj.edu> Message-ID: and I. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Geoff McAuliffe Sent: 10 May 2007 15:59 To: Gayle Callis Cc: Histonet@lists.utsouthwestern.edu Subject: Re: website for brains Re: [Histonet] brain histology When I try the site below all I get is a bunch of ads. Clicking on brain maps just gets more ads. Geoff Gayle Callis wrote: > Try this website - I am not sure they have bat, but they certainly > have many other species - it is a delight to look at. > > www.brainmaps.com > > Gayle Callis HTL, HT, MT(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University > Bozeman MT 59717 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Thu May 10 10:19:01 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Thu May 10 10:19:03 2007 Subject: [Histonet] FW: Open Biosystems products Message-ID: <002301c79316$8a98c5e0$6501a8c0@Patsy> Product specs for Open Biosystems Gayle, We have had the same problem with Open Biosystems I don't know why these things are not listed on their website. We started buying this stuff a long time ago when they were Phoenix (before that they were the company that Bragatti started), I really like their products. (we order by phone 888-412-2225), we do not have a catalog from them. Protein Blocker MBI1239 Antibody Diluent MBI1235 Hematoxylin MBI1222 Stable Dab MBI1241 -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: Thursday, May 10, 2007 8:50 AM To: Patsy Ruegg Subject: Open Biosystems products Patsy I went to Open Biosystems website and did not find any of the items you mentioned in this reply. Are there product numbers available? At 10:34 AM 5/9/2007, you wrote: >Dako's protein block is serum free and from what I understand is just >casein, no bsa. I use serum free protein block from Open Biosystems, I also >use their Stable Dab, Antibody Diluent, hematoxylin CS, etc., they are a lot >cheaper from most and I like their products. >Patsy Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From jnocito <@t> satx.rr.com Tue May 15 10:21:10 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu May 10 10:21:25 2007 Subject: [Histonet] timer calibration References: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FF97E6@sjhaexc02.sjha.org> Message-ID: <026801c79704$abc694d0$d49eae18@yourxhtr8hvc4p> I like it!! ----- Original Message ----- From: "Weems, Joyce" To: "Joe Nocito" ; "Bryan Hewlett" ; "Rene J Buesa" ; "Margiotta, Michele" ; Sent: Thursday, May 10, 2007 10:04 AM Subject: RE: [Histonet] timer calibration > Joe "The Toe Timer" ....(NOT two timer) > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 404-851-7376 - Phone > 404-851-7831 - Fax > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Joe > Nocito > Sent: Tuesday, May 15, 2007 10:58 AM > To: Bryan Hewlett; Rene J Buesa; Margiotta, Michele; > histonet@pathology.swmed.edu > Subject: Re: [Histonet] timer calibration > > > They might, > but you know me, I'm not going down without a fight!!! > I need a name change. Instead of Joe "The Toe", How about Joe "The Timer?" > I > still get to keep my initials "JTT" > Aaauggggg!! Is it Friday yet? > > JTT > ----- Original Message ----- > From: "Bryan Hewlett" > To: "Joe Nocito" ; "Rene J Buesa" > ; > "Margiotta, Michele" ; > > Sent: Wednesday, May 09, 2007 11:43 AM > Subject: Re: [Histonet] timer calibration > > >> Joe, >> >> You are talking fundamental philosophical differences here. >> The inspectors are obviously Newtonian realists, while you're leaning >> more >> to the Leibnitz/Kant view. >> They are going to win! >> >> Bryan >> >> ----- Original Message ----- >> From: "Joe Nocito" >> To: "Rene J Buesa" ; "Margiotta, Michele" >> ; >> Sent: Monday, May 14, 2007 11:08 AM >> Subject: Re: [Histonet] timer calibration >> >> >> I'm sorry >> am I the only one that thinks calibrating timers is stupid. I mean how >> many >> histology procedures are so time sensitive that the timers have to be >> calibrated? Let's face it. I have had techs sit there and watch the clock >> and rinse as soon as the timer goes off. And I've had techs wait for the >> timer to go off , then mosey over and rinse. Both stains worked. I guess >> I'm just not anal enough. >> >> JTT >> ----- Original Message ----- >> From: "Rene J Buesa" >> To: "Margiotta, Michele" ; >> >> Sent: Tuesday, May 08, 2007 3:39 PM >> Subject: Re: [Histonet] timer calibration >> >> >>> Try any local watch repair store. They have to calibrate their repaired >>> watches so it is very likely they can help you. >>> Ren? J. >>> >>> "Margiotta, Michele" wrote: >>> Hi All, >>> >>> Does anyone have a procedure for calibrating timers? We just had an >>> inspection and got cited because our timers were not calibrated. Any >>> info >>> would be appreciated! >>> >>> Michele >>> >>> >>> >>> This e-mail and any files transmitted with it are confidential and are >>> intended >>> solely for the use of the individual or entity to which they are >>> addressed. >>> This communication may contain material protected by the attorney-client >>> privilege. If you are not the intended recipient or the person >>> responsible for >>> delivering the e-mail to the intended recipient, be advised that you >>> have >>> received >>> this e-mail in error and that any use, dissemination, forwarding, >>> printing, >>> or copying of this e-mail is strictly prohibited. If you have received >>> this e-mail in >>> error, please immediately notify the sender via return e-mail or call >>> Brookhaven Memorial Hospital Medical Center at (631) 654-7282. >>> >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >>> >>> >>> >>> --------------------------------- >>> Ahhh...imagining that irresistible "new car" smell? >>> Check outnew cars at Yahoo! Autos. >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice ** The information contained in this message may be > privileged and is confidential information intended for the use of the > addressee listed above. If you are neither the intended recipient nor the > employee or agent responsible for delivering this message to the intended > recipient, you are hereby notified that any disclosure, copying, > distribution or the taking of any action in reliance on the contents of > this information is strictly prohibited. If you have received this > communication in error, please notify us immediately by replying to the > message and deleting it from your computer. Thank you. Saint Joseph's > Health System, Inc. > From gcallis <@t> montana.edu Thu May 10 10:25:28 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu May 10 10:25:36 2007 Subject: website for brains Re: [Histonet] brain histology In-Reply-To: <46433338.9060802@umdnj.edu> References: <581897.86999.qm@web50305.mail.re2.yahoo.com> <741106.41997.qm@web61225.mail.yahoo.com> <6.0.0.22.1.20070509151733.01b7ee00@gemini.msu.montana.edu> <46433338.9060802@umdnj.edu> Message-ID: <6.0.0.22.1.20070510092358.01b52e30@gemini.msu.montana.edu> Geoff and other folks, Sorry! Too many .coms out there!!! The website is www.brainmaps.org At 08:59 AM 5/10/2007, you wrote: >When I try the site below all I get is a bunch of ads. Clicking on brain >maps just gets more ads. > >Geoff > >Gayle Callis wrote: > >>Try this website - I am not sure they have bat, but they certainly have >>many other species - it is a delight to look at. >> >>www.brainmaps.com Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 From sonya.martin <@t> soton.ac.uk Thu May 10 11:25:12 2007 From: sonya.martin <@t> soton.ac.uk (Martin S.) Date: Thu May 10 11:27:24 2007 Subject: [Histonet] Crumbling frozen sections References: <71437982F5B13A4D9A5B2669BDB89EE4023E3590@ISS-CL-EX-V1.soton.ac.uk> <6.0.0.22.1.20070510085102.01b0e480@gemini.msu.montana.edu> Message-ID: <71437982F5B13A4D9A5B2669BDB89EE4023E3591@ISS-CL-EX-V1.soton.ac.uk> I have tried a range of temps from -30oC to -10 (I went up in 5oC intervals). For the colorectal tumour that was snap frozen cutting at -20oC was good its just these new ones that I'm having a problem with. The tissues are put in a plastic tube without anything else - just a blob of tissue. I'm not sure how long the tissue sits like this before I get it - the surgeon isnt very forthcoming with info! I have been using APES coated slides (which I coat myself 5% APES in Acetone, 5min, wash dH2O x3, dried in oven) and have done one test with VWR Superfrost charged slides (no difference). Thanks Sonya -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: 10 May 2007 15:55 To: Martin S. Subject: Re: [Histonet] Crumbling frozen sections Have you played with cryostat temperature to improve sectioning? It may be too cold (you did not say what temp you cut at?). Are the human tissues coming to you fresh or have they been dunked into formalin? Do they put this on saline moistened guaze to keep it from drying out? Also, what slides are you putting the tissues on, plus charge? So many questions? At 08:19 AM 5/10/2007, you wrote: >Hi All, > >I have been doing a lot of frozen sectioning of mouse tissues. I remove >the organ (spleen, lymph nodes, liver etc) from the mouse immerse it in >OCT in a foil mould and then place in a bath of isopentane on dry ice. >This has been working really well and I have been getting good sections. > >I am now looking at some human tissue (colo-rectal and liver tumours). >I receive a small piece of tissue from the surgeon which I have been >treating as for the mouse tissue. However I am finding it very hard to >get good sections. The tissue seems very flaky and crumbly and either >disintegrates on cutting or if I do get what looks like an ok section >by the time I have gone through the staining procedure it has >completely gone! > >I am not sure how long it takes between the tissue being removed and me >getting the sample - maybe 30min - could this be the cause? > >I have some old colo-rectal tumours that were snap frozen in liquid >nitrogen and they seem much better - I think I'll try doing this from >now on but just wondering if anyone has any insight into why I'm having >such problems. > >Thanks! > >Sonya > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From mpence <@t> grhs.net Thu May 10 11:49:56 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Thu May 10 11:50:08 2007 Subject: [Histonet] Crumbling frozen sections In-Reply-To: <71437982F5B13A4D9A5B2669BDB89EE4023E3591@ISS-CL-EX-V1.soton.ac.uk> Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C5C8@IS-E2K3.grhs.net> The difference you have is snap frozen tissue vs. tissue frozen by dry ice and isopentane freezing. You are more than likely getting freeze artifact from the tissue setting around in a closed tube (moisture build-up and ice crystal formation when frozen with iso.) Try handling your colorectal tissues in the same manner and see what happens. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin S. Sent: Thursday, May 10, 2007 11:25 AM To: Gayle Callis Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Crumbling frozen sections I have tried a range of temps from -30oC to -10 (I went up in 5oC intervals). For the colorectal tumour that was snap frozen cutting at -20oC was good its just these new ones that I'm having a problem with. The tissues are put in a plastic tube without anything else - just a blob of tissue. I'm not sure how long the tissue sits like this before I get it - the surgeon isnt very forthcoming with info! I have been using APES coated slides (which I coat myself 5% APES in Acetone, 5min, wash dH2O x3, dried in oven) and have done one test with VWR Superfrost charged slides (no difference). Thanks Sonya -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: 10 May 2007 15:55 To: Martin S. Subject: Re: [Histonet] Crumbling frozen sections Have you played with cryostat temperature to improve sectioning? It may be too cold (you did not say what temp you cut at?). Are the human tissues coming to you fresh or have they been dunked into formalin? Do they put this on saline moistened guaze to keep it from drying out? Also, what slides are you putting the tissues on, plus charge? So many questions? At 08:19 AM 5/10/2007, you wrote: >Hi All, > >I have been doing a lot of frozen sectioning of mouse tissues. I remove >the organ (spleen, lymph nodes, liver etc) from the mouse immerse it in >OCT in a foil mould and then place in a bath of isopentane on dry ice. >This has been working really well and I have been getting good sections. > >I am now looking at some human tissue (colo-rectal and liver tumours). >I receive a small piece of tissue from the surgeon which I have been >treating as for the mouse tissue. However I am finding it very hard to >get good sections. The tissue seems very flaky and crumbly and either >disintegrates on cutting or if I do get what looks like an ok section >by the time I have gone through the staining procedure it has >completely gone! > >I am not sure how long it takes between the tissue being removed and me >getting the sample - maybe 30min - could this be the cause? > >I have some old colo-rectal tumours that were snap frozen in liquid >nitrogen and they seem much better - I think I'll try doing this from >now on but just wondering if anyone has any insight into why I'm having >such problems. > >Thanks! > >Sonya > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vazquezr <@t> ohsu.edu Thu May 10 12:18:34 2007 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Thu May 10 12:19:20 2007 Subject: [Histonet] timer calibration Message-ID: Joe, I hear the "timer vendors" calling....ring ring!! Robyn >>> "Joe Nocito" 5/15/2007 8:05 AM >>> I have the uncanny way of starting trouble, don't I? I hope the clock police are not monitoring my emails. I might get banned again. JTT ----- Original Message ----- From: "Laurie Reilly" To: "'Blazek, Linda'" ; "'Ingles Claire'" ; Sent: Thursday, May 10, 2007 2:55 AM Subject: RE: [Histonet] timer calibration > The timer in your head probably doesn't need calibrating. > How often do we set a timer for a stain that we do regularly and then get > busy with something else? All of a sudden we think "that timer should have > gone off" and when we take it from our pocket there's 2 seconds to go. > > Regards, Laurie. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, > Linda > Sent: Thursday, 10 May 2007 5:11 AM > To: Ingles Claire; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] timer calibration > > I can't figure out how to calibrate the timer that lives in my head. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles > Claire > Sent: Wednesday, May 09, 2007 3:02 PM > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] timer calibration > > > Why don't we all just get atomic clocks and be done with it. I don't > believe they ever need to be calibrated. (unless the laws of physics and > radioactive element half-lives suddenly change). I agree with Joe. > Staining is a special talent anyway. I have had to reset timers to add > more incubation time on stains lots of times (especially silver). Oh > sorry, it's only Wednesday. Only two more days to go. > > Claire Ingles > UW Hospital > Madison WI > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Perry, > Margaret > Sent: Wed 5/9/2007 12:42 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] timer calibration > > > > We calibrate our timers by doing the following. Use the telephone to > call 303-499-7111. A voice will prompt you and tell you the time. At > the minute turn on the timer and record the Coordinated Universal time > and the timer time. Listen until the next minute and turn off the timer > and record the Coordinated Universal Time and the time on the timer. > > > > > > Margaret Perry HT (ASCP) > > IHC Lab Manager Veterinary Science > > Animal Disease Research and Diagnostic Lab > > South Dakota State University > > Box 2175 North Campus Drive > > Brookings SD 57007 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kshimp <@t> seattlecca.org Thu May 10 12:56:36 2007 From: kshimp <@t> seattlecca.org (Shimp, Kristen R) Date: Thu May 10 12:56:48 2007 Subject: [Histonet] RE: timer calibration In-Reply-To: <200705091700.l49H0suc025791@astro.seattlecca.org> Message-ID: <4EA6CBCAB26218408EFCEC255A88624101E57164@storm.seattlecca.org> What CAP question number were you sited? I haven't heard of this one pertaining to histology timers. Kristen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, May 09, 2007 10:01 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 42, Issue 11 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Placentas (Cindy DuBois) 2. protein block (Till, Renee) 3. Re: protein block (Gayle Callis) 4. timer calibration (Margiotta, Michele) 5. If you wanted an antibody what would it be? (Patricia Adams) 6. Re: timer calibration (Rene J Buesa) 7. RE: [SPAM] [Histonet] reprocessing paraffin blocks (Mickie Johnson) 8. RE: Regulatory Tcells (FoxP3) IHC (C.M. van der Loos) 9. RE: Leishmania (C.M. van der Loos) 10. FW: Her2 question (Cohen, Sherene B.) 11. Re: FW: Her2 question (Rene J Buesa) 12. Re: timer calibration (Joe Nocito) 13. RE: timer calibration (Mighnon Lashus) 14. RE: timer calibration (Douglas D Deltour) 15. Alizarin Red staining for mineralization nodules (docqian) 16. Leica ASP300 Tissue Processor for sale (Brian Branton) 17. RE: timer calibration (Mitchell Jean A.) 18. RE: timer calibration (Edwards, R.E.) 19. RE: protein block (Patsy Ruegg) 20. RE: timer calibration (Mike Pence) 21. (no subject) (Kennedy, Lisa) 22. Re: timer calibration (Bryan Hewlett) 23. RE: (no subject) (Mike Pence) ---------------------------------------------------------------------- Message: 1 Date: Tue, 8 May 2007 10:27:35 -0700 (PDT) From: Cindy DuBois Subject: [Histonet] Placentas To: Histonet Message-ID: <769673.20813.qm@web33409.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 We do this for one of our hospitals. We do charge the patient / insurance for a "Gross Only" on the placentas we accession as a gross only specimen. We were told that since the child and / or parents can sue the hospital up to 20 years later for any perceived problems that may have arisen out of the pregnancy or labor, this was to cover the hospital legally. The only hassle we have with this is how to store all the blocks we are producing. Cindy DuBois Stockton, CA --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. ------------------------------ Message: 2 Date: Tue, 8 May 2007 13:36:38 -0500 From: "Till, Renee" Subject: [Histonet] protein block To: histonet@lists.utsouthwestern.edu Message-ID: <11F927674DEBDC43B960809A7403C5D204550AA5@MAILPED.ad.uams.edu> Content-Type: text/plain; charset=us-ascii I have a protocol from a paper that I need to try and reproduce. They used the Protein Block from Dako, along with the LSAB+ kit. The Block seems to no longer be available. What would be an alternative? Is this the same as the serum block I would normally use? Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72202 Lab (501)364-8504 Office Fax (501)364-3161 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 3 Date: Tue, 08 May 2007 12:49:21 -0600 From: Gayle Callis Subject: Re: [Histonet] protein block To: "Till, Renee" , Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20070508124600.01b06170@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Renee, I doubt it, sometimes protein blocks are serum-free blocks, and may contain BSA and/or casein. I would assume another company's block should do the job as well. Try Lab Vision, Biocare, etc to see what they have available. At 12:36 PM 5/8/2007, you wrote: >I have a protocol from a paper that I need to try and reproduce. They used >the Protein Block from Dako, along with the LSAB+ kit. The Block seems to no >longer be available. What would be an alternative? Is this the same as the >serum block I would normally use? > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 ------------------------------ Message: 4 Date: Tue, 8 May 2007 15:42:12 -0400 From: "Margiotta, Michele" Subject: [Histonet] timer calibration To: Message-ID: <922CE5B88F398948B4076A9A4340E7AF036AF5FF@bmh_exchange.bmhmc.org> Content-Type: text/plain; charset="iso-8859-1" Hi All, Does anyone have a procedure for calibrating timers? We just had an inspection and got cited because our timers were not calibrated. Any info would be appreciated! Michele This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. ------------------------------ Message: 5 Date: Tue, 8 May 2007 13:11:02 -0700 (PDT) From: Patricia Adams Subject: [Histonet] If you wanted an antibody what would it be? To: HistoNet Message-ID: <102360.41177.qm@web52512.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 First I wish to thank everyone for helping me with my species antibody problems. My question is for all of you other Vet School and Vet clinic people. Do you have problems finding certain types of antibodies that will react on certain species, if so what antibody and which species? I am trying to compile a list of the most needed antibodies that we need on the animal side of things. I do again wish to thank all who sent me help, I have been burning up the Internet looking up all the information sent my way. And trying to dodge my Pathologist and all his questions! (He knows all my hiding places ;(, bummer!). Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com ------------------------------ Message: 6 Date: Tue, 8 May 2007 13:39:47 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] timer calibration To: "Margiotta, Michele" , histonet@pathology.swmed.edu Message-ID: <113979.14134.qm@web61212.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Try any local watch repair store. They have to calibrate their repaired watches so it is very likely they can help you. Ren? J. "Margiotta, Michele" wrote: Hi All, Does anyone have a procedure for calibrating timers? We just had an inspection and got cited because our timers were not calibrated. Any info would be appreciated! Michele This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. ------------------------------ Message: 7 Date: Tue, 8 May 2007 18:32:59 -0700 From: "Mickie Johnson" Subject: RE: [SPAM] [Histonet] reprocessing paraffin blocks To: "'Gayle Callis'" , Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Everyone. Thanks Gail for remembering my small article in Histo-Logic about 5 years ago! The procedure is very simple and the least harsh of any method I know. I did not develop it, but one of my former students brought it back from NSH and we used it with great success. First, melt the block down and blot off the excess paraffin from the tissue. Second, re-cassette the tissue in the same cassette, blotting excess paraffin from the cassette. Third, put the cassette in with the days normal tissue processing run, in formalin. Fourth, process as usual. The next morning, embed and cut. The fat (or under processed tissue will cut beautifully. The rational is that the well processed part of the block will have paraffin in it and will not feel the effects of dehydration. Xylene will melt out the paraffin and then paraffin will re-infiltrate this part. The unprocessed tissue areas are available to fix additionally and dehydrate, clear and infiltrate with paraffin. The net result is a reprocessed block with no harsh treatment and very little time expended to 'reprocess' by hand. It does take overnight to get the slides, but usually the pathologist will be happy to see slides he can read accurately. Hope this helps. Good Luck! Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Friday, May 04, 2007 9:18 AM To: Histonet@lists.utsouthwestern.edu Subject: [SPAM] [Histonet] reprocessing paraffin blocks Get this publication from Histo Logic on Sakura Finetek website. It was very simple and very little work overall. A Technique for Correcting Poorly Processed Paraffin Blocks. Michael L. Johnson, BS, HTL, HT(ASCP), Spokane, WA, May 2003;XXXVI(1):21. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Wed, 09 May 2007 09:19:03 +0200 From: "C.M. van der Loos" Subject: [Histonet] RE: Regulatory Tcells (FoxP3) IHC To: histonet@lists.utsouthwestern.edu Cc: Melissa.Gonzalez@cellgenesys.com, mauger@email.chop.edu Message-ID: <132f4512faf0.12faf0132f45@amc.uva.nl> Content-Type: text/plain; charset="us-ascii" Melissa, We used the Abcam mouse antibody, clone 236A/E7 we great success on both human cryo and FFPE samples, and also in double staining with CD25, CD4 (cryo). For more details please see our paper recently accepted for JHC: Onno J. de Boer et al., Immunohistochemical Analysis of Regulatory T Cell Markers FOXP3 and GITR on CD4^+CD25^+ T Cells in Normal Skin and Inflammatory Dermatoses. Go to the JHC website ([1]www.jhc.org) and go to exPRESS. You will find our paper under: May 3rd 2007. Cheers, Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Mon, 7 May 2007 10:35:52 -0700 From: "Melissa Gonzalez" Subject: [Histonet] Regulatory Tcells (FoxP3) IHC To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi all, Anyone have any experience with this? How well does the Abcam antibody work on FFPE human tissues? What's a good positive control? Thanks Melissa References 1. http://www.jhc.org/ ------------------------------ Message: 9 Date: Wed, 09 May 2007 09:50:37 +0200 From: "C.M. van der Loos" Subject: [Histonet] RE: Leishmania To: histonet@pathology.swmed.edu Cc: SDrew@uwhealth.org Message-ID: Content-Type: text/plain; charset="us-ascii" Dear Sally, Last year I tried an antibody from Cedarlane Leishmania LPG, clone CA7AE (1:500, 60 min, RT). I could make it work with HIER citrate pH6.0 and indirect fluorescence on FFPE samples. Spectral imaging was used for unmixing "real" signal from autofluorescence. Even then it was hard to find the specific signal, due to some specific-looking nuclear background staining. The tissue samples that were positive with fluorescence were also subjected to IHC with anti-mouse polymers and DAB or LPR as chromogens. This totally failed and we gave up. I realize it's not a very hopeful story but perhaps it helps anyway. Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Mon, 7 May 2007 15:03:28 -0500 From: "Drew Sally A." Subject: [Histonet] Leishmania To: "Histonet" Could someone direct us to a place/person who might run an antibody against Leishmania? We have a pathologist asking questions about it, and all our usual source don't list it. Thank you! Sally Ann Drew, MT(ASCP) IHC/ISH Laboratory University of Wisconsin Hosp. & Clinics Madison, WI 53792 ------------------------------ Message: 10 Date: Wed, 9 May 2007 09:30:05 -0400 From: "Cohen, Sherene B." Subject: [Histonet] FW: Her2 question To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <670B8345AF238F40910FEC4CA7D4B7D2F9E991@exchserver.fccc.edu> Content-Type: text/plain; charset="iso-8859-1" > -----Original Message----- > From: Cohen, Sherene B. > Sent: Tuesday, May 08, 2007 3:12 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: Her2 question > > Hey netters, > > My director just asked me about fixation in NBF and Her2. I'm curious to > know what others are doing about: > > A) Weekend processing of breast specimens > > B) If anyone has studied the antigenicity effect of these specimens > sitting in warm paraffin for at least 8 hours. > > Any feedeback is greatly appreciated. > > Sherene > > ------------------------------ Message: 11 Date: Wed, 9 May 2007 07:35:01 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] FW: Her2 question To: "Cohen, Sherene B." , "'histonet@lists.utsouthwestern.edu'" Message-ID: <222753.88085.qm@web61222.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 This was the subject of an extense discussion in Histonet just one month ago. It would be better for you to look in the archieves and benefit from that discussion. Ren? J. "Cohen, Sherene B." wrote: > -----Original Message----- > From: Cohen, Sherene B. > Sent: Tuesday, May 08, 2007 3:12 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: Her2 question > > Hey netters, > > My director just asked me about fixation in NBF and Her2. I'm curious to > know what others are doing about: > > A) Weekend processing of breast specimens > > B) If anyone has studied the antigenicity effect of these specimens > sitting in warm paraffin for at least 8 hours. > > Any feedeback is greatly appreciated. > > Sherene > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss an email again! Yahoo! Toolbar alerts you the instant new Mail arrives. Check it out. ------------------------------ Message: 12 Date: Mon, 14 May 2007 10:08:30 -0500 From: "Joe Nocito" Subject: Re: [Histonet] timer calibration To: "Rene J Buesa" , "Margiotta, Michele" , Message-ID: <00c801c79639$bc0a91a0$d49eae18@yourxhtr8hvc4p> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original I'm sorry am I the only one that thinks calibrating timers is stupid. I mean how many histology procedures are so time sensitive that the timers have to be calibrated? Let's face it. I have had techs sit there and watch the clock and rinse as soon as the timer goes off. And I've had techs wait for the timer to go off , then mosey over and rinse. Both stains worked. I guess I'm just not anal enough. JTT ----- Original Message ----- From: "Rene J Buesa" To: "Margiotta, Michele" ; Sent: Tuesday, May 08, 2007 3:39 PM Subject: Re: [Histonet] timer calibration > Try any local watch repair store. They have to calibrate their repaired > watches so it is very likely they can help you. > Ren? J. > > "Margiotta, Michele" wrote: > Hi All, > > Does anyone have a procedure for calibrating timers? We just had an > inspection and got cited because our timers were not calibrated. Any info > would be appreciated! > > Michele > > > > This e-mail and any files transmitted with it are confidential and are > intended > solely for the use of the individual or entity to which they are > addressed. > This communication may contain material protected by the attorney-client > privilege. If you are not the intended recipient or the person responsible > for > delivering the e-mail to the intended recipient, be advised that you have > received > this e-mail in error and that any use, dissemination, forwarding, > printing, > or copying of this e-mail is strictly prohibited. If you have received > this e-mail in > error, please immediately notify the sender via return e-mail or call > Brookhaven Memorial Hospital Medical Center at (631) 654-7282. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Ahhh...imagining that irresistible "new car" smell? > Check outnew cars at Yahoo! Autos. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Wed, 9 May 2007 10:18:51 -0500 From: "Mighnon Lashus" Subject: RE: [Histonet] timer calibration To: Message-ID: <7DFAF4868AAAC34C986DF7E1AC16D02601156D54@pgnexchg1.pathgroup.com> Content-Type: text/plain; charset="iso-8859-1" Who sited you? Mighnon -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Monday, May 14, 2007 11:09 AM To: Rene J Buesa; Margiotta, Michele; histonet@pathology.swmed.edu Subject: Re: [Histonet] timer calibration I'm sorry am I the only one that thinks calibrating timers is stupid. I mean how many histology procedures are so time sensitive that the timers have to be calibrated? Let's face it. I have had techs sit there and watch the clock and rinse as soon as the timer goes off. And I've had techs wait for the timer to go off , then mosey over and rinse. Both stains worked. I guess I'm just not anal enough. JTT ----- Original Message ----- From: "Rene J Buesa" To: "Margiotta, Michele" ; Sent: Tuesday, May 08, 2007 3:39 PM Subject: Re: [Histonet] timer calibration > Try any local watch repair store. They have to calibrate their repaired > watches so it is very likely they can help you. > Ren? J. > > "Margiotta, Michele" wrote: > Hi All, > > Does anyone have a procedure for calibrating timers? We just had an > inspection and got cited because our timers were not calibrated. Any info > would be appreciated! > > Michele > > > > This e-mail and any files transmitted with it are confidential and are > intended > solely for the use of the individual or entity to which they are > addressed. > This communication may contain material protected by the attorney-client > privilege. If you are not the intended recipient or the person responsible > for > delivering the e-mail to the intended recipient, be advised that you have > received > this e-mail in error and that any use, dissemination, forwarding, > printing, > or copying of this e-mail is strictly prohibited. If you have received > this e-mail in > error, please immediately notify the sender via return e-mail or call > Brookhaven Memorial Hospital Medical Center at (631) 654-7282. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Ahhh...imagining that irresistible "new car" smell? > Check outnew cars at Yahoo! Autos. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc. immediately at 615-562-9255. ------------------------------ Message: 14 Date: Wed, 9 May 2007 11:25:31 -0500 From: "Douglas D Deltour" Subject: RE: [Histonet] timer calibration To: "'Joe Nocito'" , Message-ID: <1033394974-763687002@pathology.swmed.edu> Content-Type: text/plain; charset="iso-8859-1" Who inspects the calibrating timers? What are the calibrating timers calibrated against? What are the calibrating timers of the calibrating timers calibrated against? Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Monday, May 14, 2007 10:09 AM To: Rene J Buesa; Margiotta, Michele; histonet@pathology.swmed.edu Subject: Re: [Histonet] timer calibration I'm sorry am I the only one that thinks calibrating timers is stupid. I mean how many histology procedures are so time sensitive that the timers have to be calibrated? Let's face it. I have had techs sit there and watch the clock and rinse as soon as the timer goes off. And I've had techs wait for the timer to go off , then mosey over and rinse. Both stains worked. I guess I'm just not anal enough. JTT ----- Original Message ----- From: "Rene J Buesa" To: "Margiotta, Michele" ; Sent: Tuesday, May 08, 2007 3:39 PM Subject: Re: [Histonet] timer calibration > Try any local watch repair store. They have to calibrate their repaired > watches so it is very likely they can help you. > Ren? J. > > "Margiotta, Michele" wrote: > Hi All, > > Does anyone have a procedure for calibrating timers? We just had an > inspection and got cited because our timers were not calibrated. Any info > would be appreciated! > > Michele > > > > This e-mail and any files transmitted with it are confidential and are > intended > solely for the use of the individual or entity to which they are > addressed. > This communication may contain material protected by the attorney-client > privilege. If you are not the intended recipient or the person responsible > for > delivering the e-mail to the intended recipient, be advised that you have > received > this e-mail in error and that any use, dissemination, forwarding, > printing, > or copying of this e-mail is strictly prohibited. If you have received > this e-mail in > error, please immediately notify the sender via return e-mail or call > Brookhaven Memorial Hospital Medical Center at (631) 654-7282. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Ahhh...imagining that irresistible "new car" smell? > Check outnew cars at Yahoo! Autos. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Wed, 9 May 2007 23:24:51 +0800 (CST) From: docqian Subject: [Histonet] Alizarin Red staining for mineralization nodules To: Histonet@lists.utsouthwestern.edu Message-ID: <628956.75919.qm@web15210.mail.cnb.yahoo.com> Content-Type: text/plain; charset=gb2312 Dear all, I would like to perform Alizarin Red staining for mineralization nodules in primary osteoblasts, Does anyone have a protocol about it? In addition, for mineralization nodule formation, we should add ascorbic acid and beta-glycerolphosphate into the culture medium, my question is : use which solution to dissovle these drugs (distilled water, or culture medium). Thank you. Guofeng ___________________________________________________________ ????????????????3.5G??????20M?????? http://cn.mail.yahoo.com ------------------------------ Message: 16 Date: Wed, 9 May 2007 11:30:39 -0400 From: "Brian Branton" Subject: [Histonet] Leica ASP300 Tissue Processor for sale To: Message-ID: <2A5183C7E289F646A4744C85841587BF04128A@timeclock.sarapath.com> Content-Type: text/plain; charset="iso-8859-1" Hello Histonet, We are selling one of our Leica ASP300 tissue processors. If anyone is interested, please checkout our eBay listing http://cgi.ebay.com/Leica-ASP300-Tissue-Processor_W0QQitemZ200106385547QQihZ010QQcategoryZ11816QQssPageNameZWDVWQQrdZ1QQcmdZViewItem. Thank You Brian Branton Purchasing Agent Sarasota Pathology (941) 362-8963 (941) 362-8964 FAX ------------------------------ Message: 17 Date: Wed, 9 May 2007 10:45:00 -0500 From: "Mitchell Jean A." Subject: RE: [Histonet] timer calibration To: Message-ID: <936BDBD9AB6ED84FB1FD25FD55DCDFB1035585A2@uwhis-xchng4.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="us-ascii" JCAHO inspectors each and every time have inquired about my timer calibrations. I satisfy this requirement by annually calibrating my timers to my wall clock; with the wall clocks calibrated to Greenwich Mean Time (from a website) Jean Mitchell, BS HT (ASCP) University of Wisconsin Hospital & Clinics Neuromuscular Laboratory Manager 600 Highland Avenue Madison, WI 53792-5132 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Wednesday, May 09, 2007 11:26 AM To: 'Joe Nocito'; histonet@pathology.swmed.edu Subject: RE: [Histonet] timer calibration Who inspects the calibrating timers? What are the calibrating timers calibrated against? What are the calibrating timers of the calibrating timers calibrated against? Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ------------------------------ Message: 18 Date: Wed, 9 May 2007 16:58:38 +0100 From: "Edwards, R.E." Subject: RE: [Histonet] timer calibration To: "Joe Nocito" , "Rene J Buesa" , "Margiotta, Michele" , Message-ID: Content-Type: text/plain; charset="iso-8859-1" Calibration of timers is essential, as one of the least publicised consequences of global warming is that time is speeding up by about a second a year( Wells et al;Journal of Man and Time Vol 6, 211-221, 2008). -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: 14 May 2007 16:09 To: Rene J Buesa; Margiotta, Michele; histonet@pathology.swmed.edu Subject: Re: [Histonet] timer calibration I'm sorry am I the only one that thinks calibrating timers is stupid. I mean how many histology procedures are so time sensitive that the timers have to be calibrated? Let's face it. I have had techs sit there and watch the clock and rinse as soon as the timer goes off. And I've had techs wait for the timer to go off , then mosey over and rinse. Both stains worked. I guess I'm just not anal enough. JTT ----- Original Message ----- From: "Rene J Buesa" To: "Margiotta, Michele" ; Sent: Tuesday, May 08, 2007 3:39 PM Subject: Re: [Histonet] timer calibration > Try any local watch repair store. They have to calibrate their > repaired watches so it is very likely they can help you. > Ren? J. > > "Margiotta, Michele" wrote: > Hi All, > > Does anyone have a procedure for calibrating timers? We just had an > inspection and got cited because our timers were not calibrated. Any > info would be appreciated! > > Michele > > > > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to which they > are addressed. > This communication may contain material protected by the > attorney-client privilege. If you are not the intended recipient or > the person responsible for delivering the e-mail to the intended > recipient, be advised that you have received this e-mail in error and > that any use, dissemination, forwarding, printing, or copying of this > e-mail is strictly prohibited. If you have received this e-mail in > error, please immediately notify the sender via return e-mail or call > Brookhaven Memorial Hospital Medical Center at (631) 654-7282. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Ahhh...imagining that irresistible "new car" smell? > Check outnew cars at Yahoo! Autos. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Wed, 9 May 2007 10:34:31 -0600 From: "Patsy Ruegg" Subject: RE: [Histonet] protein block To: "'Gayle Callis'" , "'Till, Renee'" , Message-ID: <002701c79257$ec227640$6501a8c0@Patsy> Content-Type: text/plain; charset="us-ascii" Dako's protein block is serum free and from what I understand is just casein, no bsa. I use serum free protein block from Open Biosystems, I also use their Stable Dab, Antibody Diluent, hematoxylin CS, etc., they are a lot cheaper from most and I like their products. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Tuesday, May 08, 2007 12:49 PM To: Till, Renee; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] protein block Renee, I doubt it, sometimes protein blocks are serum-free blocks, and may contain BSA and/or casein. I would assume another company's block should do the job as well. Try Lab Vision, Biocare, etc to see what they have available. At 12:36 PM 5/8/2007, you wrote: >I have a protocol from a paper that I need to try and reproduce. They used >the Protein Block from Dako, along with the LSAB+ kit. The Block seems to no >longer be available. What would be an alternative? Is this the same as the >serum block I would normally use? > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Wed, 9 May 2007 11:40:13 -0500 From: "Mike Pence" Subject: RE: [Histonet] timer calibration To: "Edwards, R.E." , "Joe Nocito" , "Rene J Buesa" , "Margiotta, Michele" , Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C5C6@IS-E2K3.grhs.net> Content-Type: text/plain; charset="iso-8859-1" If we are going to ask if our timers are calibrated in the Histology Lab, then one could ask the question: "Do you calibrate the timers on your various automated instruments such as processors, stainers, cryostats, and the list could go on? I don't feel that 1 second a year is going to make a difference in my calibrated-timed tissue being processed or my H&E stains. If time does matter that precisely to you, then you most likely have a system in place for calibrating your timers. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, R.E. Sent: Wednesday, May 09, 2007 10:59 AM To: Joe Nocito; Rene J Buesa; Margiotta, Michele; histonet@pathology.swmed.edu Subject: RE: [Histonet] timer calibration Calibration of timers is essential, as one of the least publicised consequences of global warming is that time is speeding up by about a second a year( Wells et al;Journal of Man and Time Vol 6, 211-221, 2008). -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: 14 May 2007 16:09 To: Rene J Buesa; Margiotta, Michele; histonet@pathology.swmed.edu Subject: Re: [Histonet] timer calibration I'm sorry am I the only one that thinks calibrating timers is stupid. I mean how many histology procedures are so time sensitive that the timers have to be calibrated? Let's face it. I have had techs sit there and watch the clock and rinse as soon as the timer goes off. And I've had techs wait for the timer to go off , then mosey over and rinse. Both stains worked. I guess I'm just not anal enough. JTT ----- Original Message ----- From: "Rene J Buesa" To: "Margiotta, Michele" ; Sent: Tuesday, May 08, 2007 3:39 PM Subject: Re: [Histonet] timer calibration > Try any local watch repair store. They have to calibrate their > repaired watches so it is very likely they can help you. > Ren? J. > > "Margiotta, Michele" wrote: > Hi All, > > Does anyone have a procedure for calibrating timers? We just had an > inspection and got cited because our timers were not calibrated. Any > info would be appreciated! > > Michele > > > > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to which they > are addressed. > This communication may contain material protected by the > attorney-client privilege. If you are not the intended recipient or > the person responsible for delivering the e-mail to the intended > recipient, be advised that you have received this e-mail in error and > that any use, dissemination, forwarding, printing, or copying of this > e-mail is strictly prohibited. If you have received this e-mail in > error, please immediately notify the sender via return e-mail or call > Brookhaven Memorial Hospital Medical Center at (631) 654-7282. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Ahhh...imagining that irresistible "new car" smell? > Check outnew cars at Yahoo! Autos. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 21 Date: Wed, 9 May 2007 11:41:06 -0500 From: "Kennedy, Lisa" Subject: [Histonet] (no subject) To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=iso-8859-1 I am doing a search for information regarding Histologists who perform grossing in and dictation of surgical specimens. We have, in the past couple of years, begun to do that at our facility and are trying to get an idea of the increase of pay for histologist that doing this job warrants. Can anyone out there give me some pay ranges, etc. for Histologists who perform these tasks. Thanks so much. Lisa Kennedy, HT(ASCP) Good Samaritan Hospital Kearney, NE 68847 ------------------------------ Message: 22 Date: Wed, 9 May 2007 12:43:11 -0400 From: "Bryan Hewlett" Subject: Re: [Histonet] timer calibration To: "Joe Nocito" , "Rene J Buesa" , "Margiotta, Michele" , Message-ID: <002d01c79259$22348380$6500a8c0@mainbox> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=response Joe, You are talking fundamental philosophical differences here. The inspectors are obviously Newtonian realists, while you're leaning more to the Leibnitz/Kant view. They are going to win! Bryan ----- Original Message ----- From: "Joe Nocito" To: "Rene J Buesa" ; "Margiotta, Michele" ; Sent: Monday, May 14, 2007 11:08 AM Subject: Re: [Histonet] timer calibration I'm sorry am I the only one that thinks calibrating timers is stupid. I mean how many histology procedures are so time sensitive that the timers have to be calibrated? Let's face it. I have had techs sit there and watch the clock and rinse as soon as the timer goes off. And I've had techs wait for the timer to go off , then mosey over and rinse. Both stains worked. I guess I'm just not anal enough. JTT ----- Original Message ----- From: "Rene J Buesa" To: "Margiotta, Michele" ; Sent: Tuesday, May 08, 2007 3:39 PM Subject: Re: [Histonet] timer calibration > Try any local watch repair store. They have to calibrate their repaired > watches so it is very likely they can help you. > Ren? J. > > "Margiotta, Michele" wrote: > Hi All, > > Does anyone have a procedure for calibrating timers? We just had an > inspection and got cited because our timers were not calibrated. Any info > would be appreciated! > > Michele > > > > This e-mail and any files transmitted with it are confidential and are > intended > solely for the use of the individual or entity to which they are > addressed. > This communication may contain material protected by the attorney-client > privilege. If you are not the intended recipient or the person responsible > for > delivering the e-mail to the intended recipient, be advised that you have > received > this e-mail in error and that any use, dissemination, forwarding, > printing, > or copying of this e-mail is strictly prohibited. If you have received > this e-mail in > error, please immediately notify the sender via return e-mail or call > Brookhaven Memorial Hospital Medical Center at (631) 654-7282. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Ahhh...imagining that irresistible "new car" smell? > Check outnew cars at Yahoo! Autos. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 23 Date: Wed, 9 May 2007 11:50:35 -0500 From: "Mike Pence" Subject: RE: [Histonet] (no subject) To: "Kennedy, Lisa" , Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C5C7@IS-E2K3.grhs.net> Content-Type: text/plain; charset="us-ascii" This is going to depend on the degree of grossing the tech does. If they only put GI type bx's in or if they do the entire gross-in of specimens to the degree a PA or Resident does. You may get a wide range of pay. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kennedy, Lisa Sent: Wednesday, May 09, 2007 11:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) I am doing a search for information regarding Histologists who perform grossing in and dictation of surgical specimens. We have, in the past couple of years, begun to do that at our facility and are trying to get an idea of the increase of pay for histologist that doing this job warrants. Can anyone out there give me some pay ranges, etc. for Histologists who perform these tasks. Thanks so much. Lisa Kennedy, HT(ASCP) Good Samaritan Hospital Kearney, NE 68847 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 42, Issue 11 **************************************** This electronic message transmission contains information which may be confidential or privileged. The information is intended to be for the use of the individual or entity named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this information is prohibited. If you have received this electronic transmission in error, please leave a message via telephone at (206) 288-6266, notify me by electronic reply, and delete this message. Opinions and ideas in this message that do not relate to official business are understood as neither given nor endorsed by the Seattle Cancer Care Alliance. To view our complete Notice of Privacy Practices, visit our web site at www.seattlecca.org. From froyer <@t> bitstream.net Thu May 10 13:44:27 2007 From: froyer <@t> bitstream.net (Ford Royer) Date: Thu May 10 13:44:54 2007 Subject: [Histonet] timer calibration In-Reply-To: <002d01c79259$22348380$6500a8c0@mainbox> References: <113979.14134.qm@web61212.mail.yahoo.com><00c801c79639$bc0a91a0$d49eae18@yourxhtr8hvc4p> <002d01c79259$22348380$6500a8c0@mainbox> Message-ID: <001301c79333$3cb3a5d0$7701a80a@Ford> It's really not the Timer or the Inspector that is off. What screwed up the whole aspect of "Time" and "Timers" was the advent of Day Light Savings Time... which was, of course, adapted from an ancient practice by an early Norwegian bachelor farmers who settled in Minnesota. One night he noticed that the blanket on his bed was too short to cover his bare feet when he was sleeping. So he cut six inches off the top of the blanket and sewed it on to the bottom. Problem solved. But I digress... Ford M. Royer, MT(ASCP) Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL:? 612-839-1046 Phone:? 763-542-8725 Fax:? 763-546-4830 eMail:? clinicallab@minnesotamedical.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bryan Hewlett Sent: Wednesday, May 09, 2007 11:43 AM To: Joe Nocito; Rene J Buesa; Margiotta, Michele; histonet@pathology.swmed.edu Subject: Re: [Histonet] timer calibration Joe, You are talking fundamental philosophical differences here. The inspectors are obviously Newtonian realists, while you're leaning more to the Leibnitz/Kant view. They are going to win! Bryan ----- Original Message ----- From: "Joe Nocito" To: "Rene J Buesa" ; "Margiotta, Michele" ; Sent: Monday, May 14, 2007 11:08 AM Subject: Re: [Histonet] timer calibration I'm sorry am I the only one that thinks calibrating timers is stupid. I mean how many histology procedures are so time sensitive that the timers have to be calibrated? Let's face it. I have had techs sit there and watch the clock and rinse as soon as the timer goes off. And I've had techs wait for the timer to go off , then mosey over and rinse. Both stains worked. I guess I'm just not anal enough. JTT ----- Original Message ----- From: "Rene J Buesa" To: "Margiotta, Michele" ; Sent: Tuesday, May 08, 2007 3:39 PM Subject: Re: [Histonet] timer calibration > Try any local watch repair store. They have to calibrate their repaired > watches so it is very likely they can help you. > Ren? J. > > "Margiotta, Michele" wrote: > Hi All, > > Does anyone have a procedure for calibrating timers? We just had an > inspection and got cited because our timers were not calibrated. Any info > would be appreciated! > > Michele > > > > This e-mail and any files transmitted with it are confidential and are > intended > solely for the use of the individual or entity to which they are > addressed. > This communication may contain material protected by the attorney-client > privilege. If you are not the intended recipient or the person responsible > for > delivering the e-mail to the intended recipient, be advised that you have > received > this e-mail in error and that any use, dissemination, forwarding, > printing, > or copying of this e-mail is strictly prohibited. If you have received > this e-mail in > error, please immediately notify the sender via return e-mail or call > Brookhaven Memorial Hospital Medical Center at (631) 654-7282. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Ahhh...imagining that irresistible "new car" smell? > Check outnew cars at Yahoo! Autos. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Thu May 10 13:55:22 2007 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu May 10 13:55:46 2007 Subject: [Histonet] RE: timer calibration In-Reply-To: <4EA6CBCAB26218408EFCEC255A88624101E57164@storm.seattlecca.org> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB7123@EMAIL.archildrens.org> I think this is in the lab general checklist which applies to histology as well.... Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shimp, Kristen R Sent: Thursday, May 10, 2007 12:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: timer calibration What CAP question number were you sited? I haven't heard of this one pertaining to histology timers. Kristen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, May 09, 2007 10:01 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 42, Issue 11 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Placentas (Cindy DuBois) 2. protein block (Till, Renee) 3. Re: protein block (Gayle Callis) 4. timer calibration (Margiotta, Michele) 5. If you wanted an antibody what would it be? (Patricia Adams) 6. Re: timer calibration (Rene J Buesa) 7. RE: [SPAM] [Histonet] reprocessing paraffin blocks (Mickie Johnson) 8. RE: Regulatory Tcells (FoxP3) IHC (C.M. van der Loos) 9. RE: Leishmania (C.M. van der Loos) 10. FW: Her2 question (Cohen, Sherene B.) 11. Re: FW: Her2 question (Rene J Buesa) 12. Re: timer calibration (Joe Nocito) 13. RE: timer calibration (Mighnon Lashus) 14. RE: timer calibration (Douglas D Deltour) 15. Alizarin Red staining for mineralization nodules (docqian) 16. Leica ASP300 Tissue Processor for sale (Brian Branton) 17. RE: timer calibration (Mitchell Jean A.) 18. RE: timer calibration (Edwards, R.E.) 19. RE: protein block (Patsy Ruegg) 20. RE: timer calibration (Mike Pence) 21. (no subject) (Kennedy, Lisa) 22. Re: timer calibration (Bryan Hewlett) 23. RE: (no subject) (Mike Pence) ---------------------------------------------------------------------- Message: 1 Date: Tue, 8 May 2007 10:27:35 -0700 (PDT) From: Cindy DuBois Subject: [Histonet] Placentas To: Histonet Message-ID: <769673.20813.qm@web33409.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 We do this for one of our hospitals. We do charge the patient / insurance for a "Gross Only" on the placentas we accession as a gross only specimen. We were told that since the child and / or parents can sue the hospital up to 20 years later for any perceived problems that may have arisen out of the pregnancy or labor, this was to cover the hospital legally. The only hassle we have with this is how to store all the blocks we are producing. Cindy DuBois Stockton, CA --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. ------------------------------ Message: 2 Date: Tue, 8 May 2007 13:36:38 -0500 From: "Till, Renee" Subject: [Histonet] protein block To: histonet@lists.utsouthwestern.edu Message-ID: <11F927674DEBDC43B960809A7403C5D204550AA5@MAILPED.ad.uams.edu> Content-Type: text/plain; charset=us-ascii I have a protocol from a paper that I need to try and reproduce. They used the Protein Block from Dako, along with the LSAB+ kit. The Block seems to no longer be available. What would be an alternative? Is this the same as the serum block I would normally use? Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72202 Lab (501)364-8504 Office Fax (501)364-3161 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 3 Date: Tue, 08 May 2007 12:49:21 -0600 From: Gayle Callis Subject: Re: [Histonet] protein block To: "Till, Renee" , Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20070508124600.01b06170@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Renee, I doubt it, sometimes protein blocks are serum-free blocks, and may contain BSA and/or casein. I would assume another company's block should do the job as well. Try Lab Vision, Biocare, etc to see what they have available. At 12:36 PM 5/8/2007, you wrote: >I have a protocol from a paper that I need to try and reproduce. They used >the Protein Block from Dako, along with the LSAB+ kit. The Block seems to no >longer be available. What would be an alternative? Is this the same as the >serum block I would normally use? > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 ------------------------------ Message: 4 Date: Tue, 8 May 2007 15:42:12 -0400 From: "Margiotta, Michele" Subject: [Histonet] timer calibration To: Message-ID: <922CE5B88F398948B4076A9A4340E7AF036AF5FF@bmh_exchange.bmhmc.org> Content-Type: text/plain; charset="iso-8859-1" Hi All, Does anyone have a procedure for calibrating timers? We just had an inspection and got cited because our timers were not calibrated. Any info would be appreciated! Michele This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. ------------------------------ Message: 5 Date: Tue, 8 May 2007 13:11:02 -0700 (PDT) From: Patricia Adams Subject: [Histonet] If you wanted an antibody what would it be? To: HistoNet Message-ID: <102360.41177.qm@web52512.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 First I wish to thank everyone for helping me with my species antibody problems. My question is for all of you other Vet School and Vet clinic people. Do you have problems finding certain types of antibodies that will react on certain species, if so what antibody and which species? I am trying to compile a list of the most needed antibodies that we need on the animal side of things. I do again wish to thank all who sent me help, I have been burning up the Internet looking up all the information sent my way. And trying to dodge my Pathologist and all his questions! (He knows all my hiding places ;(, bummer!). Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com ------------------------------ Message: 6 Date: Tue, 8 May 2007 13:39:47 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] timer calibration To: "Margiotta, Michele" , histonet@pathology.swmed.edu Message-ID: <113979.14134.qm@web61212.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Try any local watch repair store. They have to calibrate their repaired watches so it is very likely they can help you. Ren? J. "Margiotta, Michele" wrote: Hi All, Does anyone have a procedure for calibrating timers? We just had an inspection and got cited because our timers were not calibrated. Any info would be appreciated! Michele This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. ------------------------------ Message: 7 Date: Tue, 8 May 2007 18:32:59 -0700 From: "Mickie Johnson" Subject: RE: [SPAM] [Histonet] reprocessing paraffin blocks To: "'Gayle Callis'" , Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Everyone. Thanks Gail for remembering my small article in Histo-Logic about 5 years ago! The procedure is very simple and the least harsh of any method I know. I did not develop it, but one of my former students brought it back from NSH and we used it with great success. First, melt the block down and blot off the excess paraffin from the tissue. Second, re-cassette the tissue in the same cassette, blotting excess paraffin from the cassette. Third, put the cassette in with the days normal tissue processing run, in formalin. Fourth, process as usual. The next morning, embed and cut. The fat (or under processed tissue will cut beautifully. The rational is that the well processed part of the block will have paraffin in it and will not feel the effects of dehydration. Xylene will melt out the paraffin and then paraffin will re-infiltrate this part. The unprocessed tissue areas are available to fix additionally and dehydrate, clear and infiltrate with paraffin. The net result is a reprocessed block with no harsh treatment and very little time expended to 'reprocess' by hand. It does take overnight to get the slides, but usually the pathologist will be happy to see slides he can read accurately. Hope this helps. Good Luck! Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Friday, May 04, 2007 9:18 AM To: Histonet@lists.utsouthwestern.edu Subject: [SPAM] [Histonet] reprocessing paraffin blocks Get this publication from Histo Logic on Sakura Finetek website. It was very simple and very little work overall. A Technique for Correcting Poorly Processed Paraffin Blocks. Michael L. Johnson, BS, HTL, HT(ASCP), Spokane, WA, May 2003;XXXVI(1):21. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Wed, 09 May 2007 09:19:03 +0200 From: "C.M. van der Loos" Subject: [Histonet] RE: Regulatory Tcells (FoxP3) IHC To: histonet@lists.utsouthwestern.edu Cc: Melissa.Gonzalez@cellgenesys.com, mauger@email.chop.edu Message-ID: <132f4512faf0.12faf0132f45@amc.uva.nl> Content-Type: text/plain; charset="us-ascii" Melissa, We used the Abcam mouse antibody, clone 236A/E7 we great success on both human cryo and FFPE samples, and also in double staining with CD25, CD4 (cryo). For more details please see our paper recently accepted for JHC: Onno J. de Boer et al., Immunohistochemical Analysis of Regulatory T Cell Markers FOXP3 and GITR on CD4^+CD25^+ T Cells in Normal Skin and Inflammatory Dermatoses. Go to the JHC website ([1]www.jhc.org) and go to exPRESS. You will find our paper under: May 3rd 2007. Cheers, Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Mon, 7 May 2007 10:35:52 -0700 From: "Melissa Gonzalez" Subject: [Histonet] Regulatory Tcells (FoxP3) IHC To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi all, Anyone have any experience with this? How well does the Abcam antibody work on FFPE human tissues? What's a good positive control? Thanks Melissa References 1. http://www.jhc.org/ ------------------------------ Message: 9 Date: Wed, 09 May 2007 09:50:37 +0200 From: "C.M. van der Loos" Subject: [Histonet] RE: Leishmania To: histonet@pathology.swmed.edu Cc: SDrew@uwhealth.org Message-ID: Content-Type: text/plain; charset="us-ascii" Dear Sally, Last year I tried an antibody from Cedarlane Leishmania LPG, clone CA7AE (1:500, 60 min, RT). I could make it work with HIER citrate pH6.0 and indirect fluorescence on FFPE samples. Spectral imaging was used for unmixing "real" signal from autofluorescence. Even then it was hard to find the specific signal, due to some specific-looking nuclear background staining. The tissue samples that were positive with fluorescence were also subjected to IHC with anti-mouse polymers and DAB or LPR as chromogens. This totally failed and we gave up. I realize it's not a very hopeful story but perhaps it helps anyway. Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Mon, 7 May 2007 15:03:28 -0500 From: "Drew Sally A." Subject: [Histonet] Leishmania To: "Histonet" Could someone direct us to a place/person who might run an antibody against Leishmania? We have a pathologist asking questions about it, and all our usual source don't list it. Thank you! Sally Ann Drew, MT(ASCP) IHC/ISH Laboratory University of Wisconsin Hosp. & Clinics Madison, WI 53792 ------------------------------ Message: 10 Date: Wed, 9 May 2007 09:30:05 -0400 From: "Cohen, Sherene B." Subject: [Histonet] FW: Her2 question To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <670B8345AF238F40910FEC4CA7D4B7D2F9E991@exchserver.fccc.edu> Content-Type: text/plain; charset="iso-8859-1" > -----Original Message----- > From: Cohen, Sherene B. > Sent: Tuesday, May 08, 2007 3:12 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: Her2 question > > Hey netters, > > My director just asked me about fixation in NBF and Her2. I'm curious to > know what others are doing about: > > A) Weekend processing of breast specimens > > B) If anyone has studied the antigenicity effect of these specimens > sitting in warm paraffin for at least 8 hours. > > Any feedeback is greatly appreciated. > > Sherene > > ------------------------------ Message: 11 Date: Wed, 9 May 2007 07:35:01 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] FW: Her2 question To: "Cohen, Sherene B." , "'histonet@lists.utsouthwestern.edu'" Message-ID: <222753.88085.qm@web61222.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 This was the subject of an extense discussion in Histonet just one month ago. It would be better for you to look in the archieves and benefit from that discussion. Ren? J. "Cohen, Sherene B." wrote: > -----Original Message----- > From: Cohen, Sherene B. > Sent: Tuesday, May 08, 2007 3:12 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: Her2 question > > Hey netters, > > My director just asked me about fixation in NBF and Her2. I'm curious to > know what others are doing about: > > A) Weekend processing of breast specimens > > B) If anyone has studied the antigenicity effect of these specimens > sitting in warm paraffin for at least 8 hours. > > Any feedeback is greatly appreciated. > > Sherene > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss an email again! Yahoo! Toolbar alerts you the instant new Mail arrives. Check it out. ------------------------------ Message: 12 Date: Mon, 14 May 2007 10:08:30 -0500 From: "Joe Nocito" Subject: Re: [Histonet] timer calibration To: "Rene J Buesa" , "Margiotta, Michele" , Message-ID: <00c801c79639$bc0a91a0$d49eae18@yourxhtr8hvc4p> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original I'm sorry am I the only one that thinks calibrating timers is stupid. I mean how many histology procedures are so time sensitive that the timers have to be calibrated? Let's face it. I have had techs sit there and watch the clock and rinse as soon as the timer goes off. And I've had techs wait for the timer to go off , then mosey over and rinse. Both stains worked. I guess I'm just not anal enough. JTT ----- Original Message ----- From: "Rene J Buesa" To: "Margiotta, Michele" ; Sent: Tuesday, May 08, 2007 3:39 PM Subject: Re: [Histonet] timer calibration > Try any local watch repair store. They have to calibrate their repaired > watches so it is very likely they can help you. > Ren? J. > > "Margiotta, Michele" wrote: > Hi All, > > Does anyone have a procedure for calibrating timers? We just had an > inspection and got cited because our timers were not calibrated. Any info > would be appreciated! > > Michele > > > > This e-mail and any files transmitted with it are confidential and are > intended > solely for the use of the individual or entity to which they are > addressed. > This communication may contain material protected by the attorney-client > privilege. If you are not the intended recipient or the person responsible > for > delivering the e-mail to the intended recipient, be advised that you have > received > this e-mail in error and that any use, dissemination, forwarding, > printing, > or copying of this e-mail is strictly prohibited. If you have received > this e-mail in > error, please immediately notify the sender via return e-mail or call > Brookhaven Memorial Hospital Medical Center at (631) 654-7282. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Ahhh...imagining that irresistible "new car" smell? > Check outnew cars at Yahoo! Autos. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Wed, 9 May 2007 10:18:51 -0500 From: "Mighnon Lashus" Subject: RE: [Histonet] timer calibration To: Message-ID: <7DFAF4868AAAC34C986DF7E1AC16D02601156D54@pgnexchg1.pathgroup.com> Content-Type: text/plain; charset="iso-8859-1" Who sited you? Mighnon -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Monday, May 14, 2007 11:09 AM To: Rene J Buesa; Margiotta, Michele; histonet@pathology.swmed.edu Subject: Re: [Histonet] timer calibration I'm sorry am I the only one that thinks calibrating timers is stupid. I mean how many histology procedures are so time sensitive that the timers have to be calibrated? Let's face it. I have had techs sit there and watch the clock and rinse as soon as the timer goes off. And I've had techs wait for the timer to go off , then mosey over and rinse. Both stains worked. I guess I'm just not anal enough. JTT ----- Original Message ----- From: "Rene J Buesa" To: "Margiotta, Michele" ; Sent: Tuesday, May 08, 2007 3:39 PM Subject: Re: [Histonet] timer calibration > Try any local watch repair store. They have to calibrate their repaired > watches so it is very likely they can help you. > Ren? J. > > "Margiotta, Michele" wrote: > Hi All, > > Does anyone have a procedure for calibrating timers? We just had an > inspection and got cited because our timers were not calibrated. Any info > would be appreciated! > > Michele > > > > This e-mail and any files transmitted with it are confidential and are > intended > solely for the use of the individual or entity to which they are > addressed. > This communication may contain material protected by the attorney-client > privilege. If you are not the intended recipient or the person responsible > for > delivering the e-mail to the intended recipient, be advised that you have > received > this e-mail in error and that any use, dissemination, forwarding, > printing, > or copying of this e-mail is strictly prohibited. If you have received > this e-mail in > error, please immediately notify the sender via return e-mail or call > Brookhaven Memorial Hospital Medical Center at (631) 654-7282. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Ahhh...imagining that irresistible "new car" smell? > Check outnew cars at Yahoo! Autos. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc. immediately at 615-562-9255. ------------------------------ Message: 14 Date: Wed, 9 May 2007 11:25:31 -0500 From: "Douglas D Deltour" Subject: RE: [Histonet] timer calibration To: "'Joe Nocito'" , Message-ID: <1033394974-763687002@pathology.swmed.edu> Content-Type: text/plain; charset="iso-8859-1" Who inspects the calibrating timers? What are the calibrating timers calibrated against? What are the calibrating timers of the calibrating timers calibrated against? Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Monday, May 14, 2007 10:09 AM To: Rene J Buesa; Margiotta, Michele; histonet@pathology.swmed.edu Subject: Re: [Histonet] timer calibration I'm sorry am I the only one that thinks calibrating timers is stupid. I mean how many histology procedures are so time sensitive that the timers have to be calibrated? Let's face it. I have had techs sit there and watch the clock and rinse as soon as the timer goes off. And I've had techs wait for the timer to go off , then mosey over and rinse. Both stains worked. I guess I'm just not anal enough. JTT ----- Original Message ----- From: "Rene J Buesa" To: "Margiotta, Michele" ; Sent: Tuesday, May 08, 2007 3:39 PM Subject: Re: [Histonet] timer calibration > Try any local watch repair store. They have to calibrate their repaired > watches so it is very likely they can help you. > Ren? J. > > "Margiotta, Michele" wrote: > Hi All, > > Does anyone have a procedure for calibrating timers? We just had an > inspection and got cited because our timers were not calibrated. Any info > would be appreciated! > > Michele > > > > This e-mail and any files transmitted with it are confidential and are > intended > solely for the use of the individual or entity to which they are > addressed. > This communication may contain material protected by the attorney-client > privilege. If you are not the intended recipient or the person responsible > for > delivering the e-mail to the intended recipient, be advised that you have > received > this e-mail in error and that any use, dissemination, forwarding, > printing, > or copying of this e-mail is strictly prohibited. If you have received > this e-mail in > error, please immediately notify the sender via return e-mail or call > Brookhaven Memorial Hospital Medical Center at (631) 654-7282. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Ahhh...imagining that irresistible "new car" smell? > Check outnew cars at Yahoo! Autos. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Wed, 9 May 2007 23:24:51 +0800 (CST) From: docqian Subject: [Histonet] Alizarin Red staining for mineralization nodules To: Histonet@lists.utsouthwestern.edu Message-ID: <628956.75919.qm@web15210.mail.cnb.yahoo.com> Content-Type: text/plain; charset=gb2312 Dear all, I would like to perform Alizarin Red staining for mineralization nodules in primary osteoblasts, Does anyone have a protocol about it? In addition, for mineralization nodule formation, we should add ascorbic acid and beta-glycerolphosphate into the culture medium, my question is : use which solution to dissovle these drugs (distilled water, or culture medium). Thank you. Guofeng ___________________________________________________________ ????????????????3.5G??????20M?????? http://cn.mail.yahoo.com ------------------------------ Message: 16 Date: Wed, 9 May 2007 11:30:39 -0400 From: "Brian Branton" Subject: [Histonet] Leica ASP300 Tissue Processor for sale To: Message-ID: <2A5183C7E289F646A4744C85841587BF04128A@timeclock.sarapath.com> Content-Type: text/plain; charset="iso-8859-1" Hello Histonet, We are selling one of our Leica ASP300 tissue processors. If anyone is interested, please checkout our eBay listing http://cgi.ebay.com/Leica-ASP300-Tissue-Processor_W0QQitemZ200106385547QQihZ010QQcategoryZ11816QQssPageNameZWDVWQQrdZ1QQcmdZViewItem. Thank You Brian Branton Purchasing Agent Sarasota Pathology (941) 362-8963 (941) 362-8964 FAX ------------------------------ Message: 17 Date: Wed, 9 May 2007 10:45:00 -0500 From: "Mitchell Jean A." Subject: RE: [Histonet] timer calibration To: Message-ID: <936BDBD9AB6ED84FB1FD25FD55DCDFB1035585A2@uwhis-xchng4.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="us-ascii" JCAHO inspectors each and every time have inquired about my timer calibrations. I satisfy this requirement by annually calibrating my timers to my wall clock; with the wall clocks calibrated to Greenwich Mean Time (from a website) Jean Mitchell, BS HT (ASCP) University of Wisconsin Hospital & Clinics Neuromuscular Laboratory Manager 600 Highland Avenue Madison, WI 53792-5132 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Wednesday, May 09, 2007 11:26 AM To: 'Joe Nocito'; histonet@pathology.swmed.edu Subject: RE: [Histonet] timer calibration Who inspects the calibrating timers? What are the calibrating timers calibrated against? What are the calibrating timers of the calibrating timers calibrated against? Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ------------------------------ Message: 18 Date: Wed, 9 May 2007 16:58:38 +0100 From: "Edwards, R.E." Subject: RE: [Histonet] timer calibration To: "Joe Nocito" , "Rene J Buesa" , "Margiotta, Michele" , Message-ID: Content-Type: text/plain; charset="iso-8859-1" Calibration of timers is essential, as one of the least publicised consequences of global warming is that time is speeding up by about a second a year( Wells et al;Journal of Man and Time Vol 6, 211-221, 2008). -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: 14 May 2007 16:09 To: Rene J Buesa; Margiotta, Michele; histonet@pathology.swmed.edu Subject: Re: [Histonet] timer calibration I'm sorry am I the only one that thinks calibrating timers is stupid. I mean how many histology procedures are so time sensitive that the timers have to be calibrated? Let's face it. I have had techs sit there and watch the clock and rinse as soon as the timer goes off. And I've had techs wait for the timer to go off , then mosey over and rinse. Both stains worked. I guess I'm just not anal enough. JTT ----- Original Message ----- From: "Rene J Buesa" To: "Margiotta, Michele" ; Sent: Tuesday, May 08, 2007 3:39 PM Subject: Re: [Histonet] timer calibration > Try any local watch repair store. They have to calibrate their > repaired watches so it is very likely they can help you. > Ren? J. > > "Margiotta, Michele" wrote: > Hi All, > > Does anyone have a procedure for calibrating timers? We just had an > inspection and got cited because our timers were not calibrated. Any > info would be appreciated! > > Michele > > > > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to which they > are addressed. > This communication may contain material protected by the > attorney-client privilege. If you are not the intended recipient or > the person responsible for delivering the e-mail to the intended > recipient, be advised that you have received this e-mail in error and > that any use, dissemination, forwarding, printing, or copying of this > e-mail is strictly prohibited. If you have received this e-mail in > error, please immediately notify the sender via return e-mail or call > Brookhaven Memorial Hospital Medical Center at (631) 654-7282. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Ahhh...imagining that irresistible "new car" smell? > Check outnew cars at Yahoo! Autos. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Wed, 9 May 2007 10:34:31 -0600 From: "Patsy Ruegg" Subject: RE: [Histonet] protein block To: "'Gayle Callis'" , "'Till, Renee'" , Message-ID: <002701c79257$ec227640$6501a8c0@Patsy> Content-Type: text/plain; charset="us-ascii" Dako's protein block is serum free and from what I understand is just casein, no bsa. I use serum free protein block from Open Biosystems, I also use their Stable Dab, Antibody Diluent, hematoxylin CS, etc., they are a lot cheaper from most and I like their products. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Tuesday, May 08, 2007 12:49 PM To: Till, Renee; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] protein block Renee, I doubt it, sometimes protein blocks are serum-free blocks, and may contain BSA and/or casein. I would assume another company's block should do the job as well. Try Lab Vision, Biocare, etc to see what they have available. At 12:36 PM 5/8/2007, you wrote: >I have a protocol from a paper that I need to try and reproduce. They used >the Protein Block from Dako, along with the LSAB+ kit. The Block seems to no >longer be available. What would be an alternative? Is this the same as the >serum block I would normally use? > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Wed, 9 May 2007 11:40:13 -0500 From: "Mike Pence" Subject: RE: [Histonet] timer calibration To: "Edwards, R.E." , "Joe Nocito" , "Rene J Buesa" , "Margiotta, Michele" , Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C5C6@IS-E2K3.grhs.net> Content-Type: text/plain; charset="iso-8859-1" If we are going to ask if our timers are calibrated in the Histology Lab, then one could ask the question: "Do you calibrate the timers on your various automated instruments such as processors, stainers, cryostats, and the list could go on? I don't feel that 1 second a year is going to make a difference in my calibrated-timed tissue being processed or my H&E stains. If time does matter that precisely to you, then you most likely have a system in place for calibrating your timers. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, R.E. Sent: Wednesday, May 09, 2007 10:59 AM To: Joe Nocito; Rene J Buesa; Margiotta, Michele; histonet@pathology.swmed.edu Subject: RE: [Histonet] timer calibration Calibration of timers is essential, as one of the least publicised consequences of global warming is that time is speeding up by about a second a year( Wells et al;Journal of Man and Time Vol 6, 211-221, 2008). -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: 14 May 2007 16:09 To: Rene J Buesa; Margiotta, Michele; histonet@pathology.swmed.edu Subject: Re: [Histonet] timer calibration I'm sorry am I the only one that thinks calibrating timers is stupid. I mean how many histology procedures are so time sensitive that the timers have to be calibrated? Let's face it. I have had techs sit there and watch the clock and rinse as soon as the timer goes off. And I've had techs wait for the timer to go off , then mosey over and rinse. Both stains worked. I guess I'm just not anal enough. JTT ----- Original Message ----- From: "Rene J Buesa" To: "Margiotta, Michele" ; Sent: Tuesday, May 08, 2007 3:39 PM Subject: Re: [Histonet] timer calibration > Try any local watch repair store. They have to calibrate their > repaired watches so it is very likely they can help you. > Ren? J. > > "Margiotta, Michele" wrote: > Hi All, > > Does anyone have a procedure for calibrating timers? We just had an > inspection and got cited because our timers were not calibrated. Any > info would be appreciated! > > Michele > > > > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to which they > are addressed. > This communication may contain material protected by the > attorney-client privilege. If you are not the intended recipient or > the person responsible for delivering the e-mail to the intended > recipient, be advised that you have received this e-mail in error and > that any use, dissemination, forwarding, printing, or copying of this > e-mail is strictly prohibited. If you have received this e-mail in > error, please immediately notify the sender via return e-mail or call > Brookhaven Memorial Hospital Medical Center at (631) 654-7282. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Ahhh...imagining that irresistible "new car" smell? > Check outnew cars at Yahoo! Autos. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 21 Date: Wed, 9 May 2007 11:41:06 -0500 From: "Kennedy, Lisa" Subject: [Histonet] (no subject) To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=iso-8859-1 I am doing a search for information regarding Histologists who perform grossing in and dictation of surgical specimens. We have, in the past couple of years, begun to do that at our facility and are trying to get an idea of the increase of pay for histologist that doing this job warrants. Can anyone out there give me some pay ranges, etc. for Histologists who perform these tasks. Thanks so much. Lisa Kennedy, HT(ASCP) Good Samaritan Hospital Kearney, NE 68847 ------------------------------ Message: 22 Date: Wed, 9 May 2007 12:43:11 -0400 From: "Bryan Hewlett" Subject: Re: [Histonet] timer calibration To: "Joe Nocito" , "Rene J Buesa" , "Margiotta, Michele" , Message-ID: <002d01c79259$22348380$6500a8c0@mainbox> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=response Joe, You are talking fundamental philosophical differences here. The inspectors are obviously Newtonian realists, while you're leaning more to the Leibnitz/Kant view. They are going to win! Bryan ----- Original Message ----- From: "Joe Nocito" To: "Rene J Buesa" ; "Margiotta, Michele" ; Sent: Monday, May 14, 2007 11:08 AM Subject: Re: [Histonet] timer calibration I'm sorry am I the only one that thinks calibrating timers is stupid. I mean how many histology procedures are so time sensitive that the timers have to be calibrated? Let's face it. I have had techs sit there and watch the clock and rinse as soon as the timer goes off. And I've had techs wait for the timer to go off , then mosey over and rinse. Both stains worked. I guess I'm just not anal enough. JTT ----- Original Message ----- From: "Rene J Buesa" To: "Margiotta, Michele" ; Sent: Tuesday, May 08, 2007 3:39 PM Subject: Re: [Histonet] timer calibration > Try any local watch repair store. They have to calibrate their repaired > watches so it is very likely they can help you. > Ren? J. > > "Margiotta, Michele" wrote: > Hi All, > > Does anyone have a procedure for calibrating timers? We just had an > inspection and got cited because our timers were not calibrated. Any info > would be appreciated! > > Michele > > > > This e-mail and any files transmitted with it are confidential and are > intended > solely for the use of the individual or entity to which they are > addressed. > This communication may contain material protected by the attorney-client > privilege. If you are not the intended recipient or the person responsible > for > delivering the e-mail to the intended recipient, be advised that you have > received > this e-mail in error and that any use, dissemination, forwarding, > printing, > or copying of this e-mail is strictly prohibited. If you have received > this e-mail in > error, please immediately notify the sender via return e-mail or call > Brookhaven Memorial Hospital Medical Center at (631) 654-7282. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Ahhh...imagining that irresistible "new car" smell? > Check outnew cars at Yahoo! Autos. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 23 Date: Wed, 9 May 2007 11:50:35 -0500 From: "Mike Pence" Subject: RE: [Histonet] (no subject) To: "Kennedy, Lisa" , Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C5C7@IS-E2K3.grhs.net> Content-Type: text/plain; charset="us-ascii" This is going to depend on the degree of grossing the tech does. If they only put GI type bx's in or if they do the entire gross-in of specimens to the degree a PA or Resident does. You may get a wide range of pay. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kennedy, Lisa Sent: Wednesday, May 09, 2007 11:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) I am doing a search for information regarding Histologists who perform grossing in and dictation of surgical specimens. We have, in the past couple of years, begun to do that at our facility and are trying to get an idea of the increase of pay for histologist that doing this job warrants. Can anyone out there give me some pay ranges, etc. for Histologists who perform these tasks. Thanks so much. Lisa Kennedy, HT(ASCP) Good Samaritan Hospital Kearney, NE 68847 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 42, Issue 11 **************************************** This electronic message transmission contains information which may be confidential or privileged. The information is intended to be for the use of the individual or entity named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this information is prohibited. If you have received this electronic transmission in error, please leave a message via telephone at (206) 288-6266, notify me by electronic reply, and delete this message. Opinions and ideas in this message that do not relate to official business are understood as neither given nor endorsed by the Seattle Cancer Care Alliance. To view our complete Notice of Privacy Practices, visit our web site at www.seattlecca.org. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From nancy.troiano <@t> yale.edu Thu May 10 13:56:41 2007 From: nancy.troiano <@t> yale.edu (Nancy W. Troiano) Date: Thu May 10 13:56:51 2007 Subject: [Histonet] embedding services Message-ID: <5.2.1.1.2.20070510145536.00c0dba0@email.med.yale.edu> We embed/cut tissues in MMA here in the Yale Core Center for Musculoskeletal Disorders for outside customers. You can contact me via email at nancy.troiano@yale.edu. From nfournier <@t> sasktel.net Thu May 10 13:58:25 2007 From: nfournier <@t> sasktel.net (N Fournier) Date: Thu May 10 13:58:29 2007 Subject: [Histonet] sodium citrate antigen retrieval protocol Message-ID: Dear Colleagues, I need some assistance regarding how to do heat-induced antigen retrieval for on free-floating brain sections. I am using the protocol suggested by Jiao et al., (1999) in which they used 10 mM sodium citrate pH 8.5-9.0 for 30 min at 80 degree C. The recipe I used was 2.94 g tri-sodium citrate (dihydrate) in 1000 ml dH2O and pH the solution using 5 N NaOH. I preheated culture wells that contained sodium citrate at 80-82 degree C for about an hour or so before the antigen retrieval step was needed. We use a isotemp Fisher water bath for heating (we do not have access to a microwave). I placed netwells containing the sections into the preheated culture dish (6 well) and returned them to the heat bath. . When i used this protocol and evaluated the sections at the end of an immunostaining for synaptophysin or Prox-1, I found it produced extensive wrinkling and damage to the tissue. Any suggestions. Should I be using citric acid in the recipe for sodium citrate buffer? If so, does anyone have a recipe for the protocol and importantly the amounts of citrate acid and sodium citrate needed to produce a 10 mM solution. I appreciate your help, Neil From trinimaican2501 <@t> yahoo.com Thu May 10 14:41:27 2007 From: trinimaican2501 <@t> yahoo.com (I-sanna Gibbons) Date: Thu May 10 14:41:32 2007 Subject: [Histonet] brain histology In-Reply-To: <741106.41997.qm@web61225.mail.yahoo.com> Message-ID: <276608.99086.qm@web50302.mail.re2.yahoo.com> Thanks for your response. I have to recheck the source but after using carnoy's, i think the tissue has to be kept in alcohol... I may have to try other fixatives then.... Rene J Buesa wrote: I think is too much time in alcohol(s). Storing in absolute EthOL is not a good practice. Try to reduce this aspect and check for results. Ren? J. I-sanna Gibbons wrote: I am having a bit of trouble obtaining good quality slides of bat brains. The brains are fixed in Carnoy's, stored in absolute alcohol, processed routinely, infiltrated with paraffin, sectioned at 8 microns and stained using cresyl fast violet. The aim is to record the cytoarchitecture. The resulting slides show poor tissue integrity with extensive cracking. Can anyone suggest how this can be avoided? I was thinking maybe a double-embedding method for whole brains (10-15 mm) and thicker sections (25-30 microns)? Thanking you in advance I-sanna Gibbons, DVM Veterinary Anatomy School of Veterinary Medicine The University of the West Indies Trinidad and Tobago, W.I. --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check out new cars at Yahoo! Autos. --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From trinimaican2501 <@t> yahoo.com Thu May 10 14:51:25 2007 From: trinimaican2501 <@t> yahoo.com (I-sanna Gibbons) Date: Thu May 10 14:51:28 2007 Subject: [Histonet] brain histology In-Reply-To: <6.2.5.6.2.20070509165126.01ca4e38@vet.upenn.edu> Message-ID: <410493.95518.qm@web50302.mail.re2.yahoo.com> Will try 70% alcohol. Thanks Pamela Marcum wrote: The blocks should be stored in 70% alcohol as absolute will remove all water even the bound molecules of water you actually need. Also going into a processor for routine processing may be fixative (aqueous) through lower grades of alcohol that replace the water just removed by your storage alcohol and then remove it again. All of these factors could contribute to the cracking as the tissue is completely over exposed to alcohol. It would help to have the actual protocol used for processing. Pam Marcum (address at the bottom) At 04:40 PM 5/9/2007, you wrote: >I am having a bit of trouble obtaining good quality slides of bat >brains. The brains are fixed in Carnoy's, stored in absolute >alcohol, processed routinely, infiltrated with paraffin, sectioned >at 8 microns and stained using cresyl fast violet. The aim is to >record the cytoarchitecture. The resulting slides show poor tissue >integrity with extensive cracking. Can anyone suggest how this can >be avoided? I was thinking maybe a double-embedding method for >whole brains (10-15 mm) and thicker sections (25-30 microns)? > > Thanking you in advance > > I-sanna Gibbons, DVM > Veterinary Anatomy > School of Veterinary Medicine > The University of the West Indies > Trinidad and Tobago, W.I. > > >--------------------------------- >Ahhh...imagining that irresistible "new car" smell? > Check outnew cars at Yahoo! Autos. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From trinimaican2501 <@t> yahoo.com Thu May 10 14:56:10 2007 From: trinimaican2501 <@t> yahoo.com (I-sanna Gibbons) Date: Thu May 10 15:02:54 2007 Subject: [Histonet] brain histology In-Reply-To: <8B07D141BCDE434285DC12B3290E3FB30111E657@exbackca.caus.dako.net> Message-ID: <773140.51749.qm@web50304.mail.re2.yahoo.com> Thanks! I'm always particularly concerned about safety. The thing isI have tried other fixatives - 10% formal saline and buffered neutral formalin... poor tissue integrity as well.... Sarah Jones wrote: Why Carnoy's fixative? It contains chloroform, and therefore hardens any tissues to the point of cracking. It is also very unsafe for you, health-wise. I have been in this field for over 40 years, and worked with chloroform for quite a bit of my early days in Histology. My liver is greatly impaired today because of this. Of course, we had open processors back then, and we also didn't have the OSHA regulations for fume control that we have today. Personally, I would try a different fixative. Sarah A. Jones, HTL(ASCP)CM Dako North America, Inc. Carpinteria, CA. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of I-sanna Gibbons Sent: Wednesday, May 09, 2007 1:40 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] brain histology I am having a bit of trouble obtaining good quality slides of bat brains. The brains are fixed in Carnoy's, stored in absolute alcohol, processed routinely, infiltrated with paraffin, sectioned at 8 microns and stained using cresyl fast violet. The aim is to record the cytoarchitecture. The resulting slides show poor tissue integrity with extensive cracking. Can anyone suggest how this can be avoided? I was thinking maybe a double-embedding method for whole brains (10-15 mm) and thicker sections (25-30 microns)? Thanking you in advance I-sanna Gibbons, DVM Veterinary Anatomy School of Veterinary Medicine The University of the West Indies Trinidad and Tobago, W.I. --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From rjbuesa <@t> yahoo.com Thu May 10 14:43:51 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu May 10 15:10:39 2007 Subject: [Histonet] sodium citrate antigen retrieval protocol In-Reply-To: Message-ID: <788992.98965.qm@web61218.mail.yahoo.com> Citrate buffer pH6 recipe: Sol. A: 21.0 g of citric acid monohydrate + 1 liter of dist. water Sol. B: 29.41 g of sodium citrate dihydrated + 1 litre of dist. water To prepare 750 mL of citrate buffer: Mix 13.5 mL of Sol.A + 61.5 mL of Sol. B + distilled water UP TO 750 mL. Adjust pHto 6 (with NaOH if pH<6 or with HCl if pH>6). My question: why on free floating brain sections? Is it necessary that way? Why not on sections adhered to a glass slide? [Just my "protocolar" ignorance!]. A free floating section will undergo the wrinkling you refer to. Could you adhere the sections to a platinum loop and process them that way (as it was customary about 50 years ago for chick embryos?). Just a thought! Ren? J. N Fournier wrote: Dear Colleagues, I need some assistance regarding how to do heat-induced antigen retrieval for on free-floating brain sections. I am using the protocol suggested by Jiao et al., (1999) in which they used 10 mM sodium citrate pH 8.5-9.0 for 30 min at 80 degree C. The recipe I used was 2.94 g tri-sodium citrate (dihydrate) in 1000 ml dH2O and pH the solution using 5 N NaOH. I preheated culture wells that contained sodium citrate at 80-82 degree C for about an hour or so before the antigen retrieval step was needed. We use a isotemp Fisher water bath for heating (we do not have access to a microwave). I placed netwells containing the sections into the preheated culture dish (6 well) and returned them to the heat bath. . When i used this protocol and evaluated the sections at the end of an immunostaining for synaptophysin or Prox-1, I found it produced extensive wrinkling and damage to the tissue. Any suggestions. Should I be using citric acid in the recipe for sodium citrate buffer? If so, does anyone have a recipe for the protocol and importantly the amounts of citrate acid and sodium citrate needed to produce a 10 mM solution. I appreciate your help, Neil _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From trinimaican2501 <@t> yahoo.com Thu May 10 15:08:33 2007 From: trinimaican2501 <@t> yahoo.com (I-sanna Gibbons) Date: Thu May 10 15:11:01 2007 Subject: [Histonet] brain histology In-Reply-To: <92ADA3E1-F789-4143-AA9E-031B798E4BE2@aol.com> Message-ID: <995367.11460.qm@web50302.mail.re2.yahoo.com> I actually used 10% formalin initially. First I perfused the bat transcardially, then removed the brain and placed it in the same fixative. That was the first batch. For the second batch of bats, I removed the brain (without prior perfusion) immediately after the animal had expired and placed it in Carnoy's. Dr. Cross, glad to meet you as well. I was actually thinking of pursuing pathology after spending 3 years in the anatomy department post-graduation. Are there any vet pathology residencies you would recommend? I-sanna Cheryl Cross wrote: Hi Dr. Gibbons - Great to see a fellow vet posting!! I do some neurotox stuff in mice. What makes for a really nice FFPE section is, if you can, perfuse fixing the rodents, then removing the calvarium overlying the brain, and letting the brain sit in the skull in your fixative of choice (I use 10% NBF). Letting the brain sit for 24 hours in the fix allows you to avoid those dreaded dark angular neurons. After sitting for 24 hours I take out the brain and section routinely ... if I can't section immediately and immunohistochemistry will be performed i switch it to 70% EtOH ... but sitting in the alcohol makes the tissue very very firm. If immunohistochemistry isn't an issue I allow the brain or organs to remain in the 10% NBF. Hope this helps, let me know if you need a protocol for perfusion, etc. Cheryl Cheryl Cross, DVM, Dipl. ACVP Researcher University Corporation for Atmospheric Research College of Veterinary Medicine University of Tennessee Department of Pathology 2407 River Drive, Room A201 Knoxville, TN 37996-4542 (423) 967-2724 fax: 865-974-5616 ccross@ucar.edu On May 9, 2007, at 4:50 PM, Rene J Buesa wrote: I think is too much time in alcohol(s). Storing in absolute EthOL is not a good practice. Try to reduce this aspect and check for results. Ren? J. I-sanna Gibbons wrote: I am having a bit of trouble obtaining good quality slides of bat brains. The brains are fixed in Carnoy's, stored in absolute alcohol, processed routinely, infiltrated with paraffin, sectioned at 8 microns and stained using cresyl fast violet. The aim is to record the cytoarchitecture. The resulting slides show poor tissue integrity with extensive cracking. Can anyone suggest how this can be avoided? I was thinking maybe a double-embedding method for whole brains (10-15 mm) and thicker sections (25-30 microns)? Thanking you in advance I-sanna Gibbons, DVM Veterinary Anatomy School of Veterinary Medicine The University of the West Indies Trinidad and Tobago, W.I. --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet = --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From laurie.colbert <@t> huntingtonhospital.com Thu May 10 15:30:50 2007 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Thu May 10 15:30:58 2007 Subject: [Histonet] Slide files for binders Message-ID: <57BE698966D5C54EAE8612E8941D768301268D31@EXCHANGE3.huntingtonhospital.com> Does anyone know where I can purchase plastic "pages" that hold glass microscope slides and have three holes in the page to put it into a binder? Laurie Colbert From DennisH <@t> cookchildrens.org Thu May 10 15:41:33 2007 From: DennisH <@t> cookchildrens.org (Dennis Hahn) Date: Thu May 10 15:42:12 2007 Subject: [Histonet] Slide files for binders Message-ID: Try Market Lab. Item # ML 1067. Website is Marketlabinc.com. Listed at $36.00 for a pack of 10pgs. Dennis Dennis Hahn, HT (ASCP) Histology Lab Supervisor Cook Children's Medical Center 801 7th Avenue Ft Worth, Tx 76104-2796 682-885-6168 dennish@cookchildrens.org >>> "Laurie Colbert" 5/10/2007 3:30 PM >>> Does anyone know where I can purchase plastic "pages" that hold glass microscope slides and have three holes in the page to put it into a binder? Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------------------------------------ Cook Children's Health Care System This e-mail, facsimile, or letter and any files or attachments transmitted may contain information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Cook Children's Health Care System immediately at (682)885-4000 or via e-mail at compliance@cookchildrens.org. Cook Children's Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. ----------------------------------------------------------------------------------------------------------- From rjbuesa <@t> yahoo.com Thu May 10 15:43:25 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu May 10 15:52:35 2007 Subject: [Histonet] Slide files for binders In-Reply-To: <57BE698966D5C54EAE8612E8941D768301268D31@EXCHANGE3.huntingtonhospital.com> Message-ID: <819130.91720.qm@web61225.mail.yahoo.com> A house called "20th Century". Ren? J. Laurie Colbert wrote: Does anyone know where I can purchase plastic "pages" that hold glass microscope slides and have three holes in the page to put it into a binder? Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From trinimaican2501 <@t> yahoo.com Thu May 10 16:59:07 2007 From: trinimaican2501 <@t> yahoo.com (I-sanna Gibbons) Date: Thu May 10 17:05:53 2007 Subject: [Histonet] bat brain Message-ID: <760799.93821.qm@web50307.mail.re2.yahoo.com> Hi everyone So what is the best histological procedure for bat brains? Fixative, duration of fixation, processing schedule, section thickness, stain, staining protocol... I have checked the literature but most authors used frozen tissue, not paraffin... All help is appreciated :) I-sanna --------------------------------- Ahhh...imagining that irresistible "new car" smell? Check outnew cars at Yahoo! Autos. From Malcolm.McCallum <@t> tamut.edu Thu May 10 17:32:07 2007 From: Malcolm.McCallum <@t> tamut.edu (Malcolm McCallum) Date: Thu May 10 17:33:19 2007 Subject: [Histonet] new issue herp con bio References: Message-ID: In case your interested, the most recent issue of herpetological conservation and biology has been posted online. No histology in it this time though! http://www.herpconbio.org VISIT HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org A New Journal Published in Partnership with Partners in Amphibian and Reptile Conservation and the World Congress of Herpetology. Malcolm L. McCallum Assistant Professor Department of Biological Sciences Texas A&M University Texarkana 2600 Robison Rd. Texarkana, TX 75501 O: 1-903-223-3134 H: 1-903-791-3843 Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html Spring Teaching Schedule & Office Hours: Monday: Genetics 1-2:40 pm Office Hours 4-6 pm Landscape Ecology 6-9:40 pm Tuesday Ichthyology 10-11:40 pm Office Hours/Student Research 1-2:30 pm Seminar 2:30-3:30 pm Wednesday Genetics 1-2:40 pm Office Hours/Student Research 3-5 pm Thursday Ichthyology 10-11:40 pm Office Hours/Student Research 1-4:30 pm ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rrlopez2@aol.com Sent: Sat 4/7/2007 1:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana, Symphony Stainer Just wanted to know if anyone is using the new Symphony Stainer from Ventana, if yes what do you like or dislike about it. Thanks ************************************** See what's free at http://www.aol.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ploykasek <@t> phenopath.com Thu May 10 17:35:28 2007 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Thu May 10 17:37:07 2007 Subject: [Histonet] Blood group H antibody Message-ID: Hi all. I am looking for an antibody to Blood Group H. In the last year, we have had a clone be discontinued from 2 manufacturers. We have been using clone 92FR-A2. We use this antibody on formalin fixed, paraffin embedded tissues. I appreciate the help. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From ccross6032 <@t> aol.com Thu May 10 18:50:41 2007 From: ccross6032 <@t> aol.com (Cheryl Cross) Date: Thu May 10 18:57:16 2007 Subject: [Histonet] bat brain In-Reply-To: <760799.93821.qm@web50307.mail.re2.yahoo.com> References: <760799.93821.qm@web50307.mail.re2.yahoo.com> Message-ID: <0CD63415-DDC7-492D-8DB8-3B45ACD1879E@aol.com> hi i-sanna i know everyone will have their own opinions.....if you aren't perfusing what we typically do for necropsy cases is to remove the brain immediately and let it sit in formalin (10% NBF) whole, for 24 hours. if you touch or cut the parenchyma when the brain is still soft you increase likelihood of artifactual neuronal change. make sure that you have enough formalin in the container - the brain should be well covered (1:10 ratio is ideal). After 24 hours then we section transversely (or what some call coronally) and wrap up the cut sections in paper towels to keep the sections in order. let that wrapped and cut brain sit for at least 2-3 days in the formalin to appropriately fix (some might even put it on a shaker to keep the formalin infiltrating into the cuts). For histopath we use 6-7 um sections and H&E staining. i am not sure of the processing schedule that's standard where i am. once you have your sections sampled you can then keep the left over portions in the same soaked paper towels with just a few cc's of formalin in a zip-lock plastic back for storage (once the brain is fixed). cheryl On May 10, 2007, at 5:59 PM, I-sanna Gibbons wrote: > Hi everyone > So what is the best histological procedure for bat brains? > Fixative, duration of fixation, processing schedule, section > thickness, stain, staining protocol... I have checked the > literature but most authors used frozen tissue, not paraffin... > All help is appreciated :) > I-sanna > > > --------------------------------- > Ahhh...imagining that irresistible "new car" smell? > Check outnew cars at Yahoo! Autos. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Tue May 15 21:41:03 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu May 10 21:41:06 2007 Subject: [Histonet] timer calibration References: Message-ID: <008a01c79763$a6c3ba30$d49eae18@yourxhtr8hvc4p> well hush my mouth ----- Original Message ----- From: "Robyn Vazquez" To: ; ; ; ; Sent: Thursday, May 10, 2007 12:18 PM Subject: Re: [Histonet] timer calibration > Joe, > I hear the "timer vendors" calling....ring ring!! > > > Robyn > >>>> "Joe Nocito" 5/15/2007 8:05 AM >>> > > I have the uncanny way of starting trouble, don't I? I hope the clock > police > are not monitoring my emails. I might get banned again. > > JTT > ----- Original Message ----- > From: "Laurie Reilly" > To: "'Blazek, Linda'" ; "'Ingles > Claire'" > ; > Sent: Thursday, May 10, 2007 2:55 AM > Subject: RE: [Histonet] timer calibration > > >> The timer in your head probably doesn't need calibrating. >> How often do we set a timer for a stain that we do regularly and then > get >> busy with something else? All of a sudden we think "that timer should > have >> gone off" and when we take it from our pocket there's 2 seconds to > go. >> >> Regards, Laurie. >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Blazek, >> Linda >> Sent: Thursday, 10 May 2007 5:11 AM >> To: Ingles Claire; histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] timer calibration >> >> I can't figure out how to calibrate the timer that lives in my head. >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Ingles >> Claire >> Sent: Wednesday, May 09, 2007 3:02 PM >> To: histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] timer calibration >> >> >> Why don't we all just get atomic clocks and be done with it. I don't >> believe they ever need to be calibrated. (unless the laws of physics > and >> radioactive element half-lives suddenly change). I agree with Joe. >> Staining is a special talent anyway. I have had to reset timers to > add >> more incubation time on stains lots of times (especially silver). Oh >> sorry, it's only Wednesday. Only two more days to go. >> >> Claire Ingles >> UW Hospital >> Madison WI >> >> ________________________________ >> >> From: histonet-bounces@lists.utsouthwestern.edu on behalf of Perry, >> Margaret >> Sent: Wed 5/9/2007 12:42 PM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] timer calibration >> >> >> >> We calibrate our timers by doing the following. Use the telephone > to >> call 303-499-7111. A voice will prompt you and tell you the time. > At >> the minute turn on the timer and record the Coordinated Universal > time >> and the timer time. Listen until the next minute and turn off the > timer >> and record the Coordinated Universal Time and the time on the timer. >> >> >> >> >> >> Margaret Perry HT (ASCP) >> >> IHC Lab Manager Veterinary Science >> >> Animal Disease Research and Diagnostic Lab >> >> South Dakota State University >> >> Box 2175 North Campus Drive >> >> Brookings SD 57007 >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Tue May 15 21:42:25 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu May 10 21:44:57 2007 Subject: [Histonet] timer calibration References: <113979.14134.qm@web61212.mail.yahoo.com> <00c801c79639$bc0a91a0$d49eae18@yourxhtr8hvc4p> <002d01c79259$22348380$6500a8c0@mainbox> <001301c79333$3cb3a5d0$7701a80a@Ford> Message-ID: <008f01c79763$d698e7d0$d49eae18@yourxhtr8hvc4p> If I move to Arizona, will my timer be fixed? ----- Original Message ----- From: "Ford Royer" To: Sent: Thursday, May 10, 2007 1:44 PM Subject: RE: [Histonet] timer calibration It's really not the Timer or the Inspector that is off. What screwed up the whole aspect of "Time" and "Timers" was the advent of Day Light Savings Time... which was, of course, adapted from an ancient practice by an early Norwegian bachelor farmers who settled in Minnesota. One night he noticed that the blanket on his bed was too short to cover his bare feet when he was sleeping. So he cut six inches off the top of the blanket and sewed it on to the bottom. Problem solved. But I digress... Ford M. Royer, MT(ASCP) Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL: 612-839-1046 Phone: 763-542-8725 Fax: 763-546-4830 eMail: clinicallab@minnesotamedical.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bryan Hewlett Sent: Wednesday, May 09, 2007 11:43 AM To: Joe Nocito; Rene J Buesa; Margiotta, Michele; histonet@pathology.swmed.edu Subject: Re: [Histonet] timer calibration Joe, You are talking fundamental philosophical differences here. The inspectors are obviously Newtonian realists, while you're leaning more to the Leibnitz/Kant view. They are going to win! Bryan ----- Original Message ----- From: "Joe Nocito" To: "Rene J Buesa" ; "Margiotta, Michele" ; Sent: Monday, May 14, 2007 11:08 AM Subject: Re: [Histonet] timer calibration I'm sorry am I the only one that thinks calibrating timers is stupid. I mean how many histology procedures are so time sensitive that the timers have to be calibrated? Let's face it. I have had techs sit there and watch the clock and rinse as soon as the timer goes off. And I've had techs wait for the timer to go off , then mosey over and rinse. Both stains worked. I guess I'm just not anal enough. JTT ----- Original Message ----- From: "Rene J Buesa" To: "Margiotta, Michele" ; Sent: Tuesday, May 08, 2007 3:39 PM Subject: Re: [Histonet] timer calibration > Try any local watch repair store. They have to calibrate their repaired > watches so it is very likely they can help you. > Ren? J. > > "Margiotta, Michele" wrote: > Hi All, > > Does anyone have a procedure for calibrating timers? We just had an > inspection and got cited because our timers were not calibrated. Any info > would be appreciated! > > Michele > > > > This e-mail and any files transmitted with it are confidential and are > intended > solely for the use of the individual or entity to which they are > addressed. > This communication may contain material protected by the attorney-client > privilege. If you are not the intended recipient or the person responsible > for > delivering the e-mail to the intended recipient, be advised that you have > received > this e-mail in error and that any use, dissemination, forwarding, > printing, > or copying of this e-mail is strictly prohibited. If you have received > this e-mail in > error, please immediately notify the sender via return e-mail or call > Brookhaven Memorial Hospital Medical Center at (631) 654-7282. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Ahhh...imagining that irresistible "new car" smell? > Check outnew cars at Yahoo! Autos. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> aol.com Fri May 11 04:32:36 2007 From: rsrichmond <@t> aol.com (rsrichmond@aol.com) Date: Fri May 11 04:32:44 2007 Subject: [Histonet] Re: timer calibration In-Reply-To: <200705101228.9c54643481b3c0@rly-me07.mx.aol.com> References: <200705101228.9c54643481b3c0@rly-me07.mx.aol.com> Message-ID: <8C961C671DE24AC-1608-6EC5@mblk-d37.sysops.aol.com> If you have Internet access in your histology laboratory, a simple source of the correct time, accurate to within about a second, is the US government's clock at www.time.gov Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From ree3 <@t> leicester.ac.uk Fri May 11 04:53:25 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Fri May 11 04:53:31 2007 Subject: [Histonet] Ki 67 antibody of choice feedback. In-Reply-To: <3AD0BD3142459B4E9B12CBEAFF2B89B2046A69D5@lajamrexm01.amer.pfizer.com> References: <3AD0BD3142459B4E9B12CBEAFF2B89B2046A69D5@lajamrexm01.amer.pfizer.com> Message-ID: Recommendations:- BD Pharmingen 2 Labvision 6 DAKO 2 Many thanks for your inputs.......... From HoustonR <@t> chi.osu.edu Fri May 11 06:43:34 2007 From: HoustonR <@t> chi.osu.edu (Houston, Ronald) Date: Fri May 11 06:43:59 2007 Subject: [Histonet] Slide files for binders In-Reply-To: <57BE698966D5C54EAE8612E8941D768301268D31@EXCHANGE3.huntingtonhospital.com> Message-ID: <979FF5962E234F45B06CF0DB7C1AABB20D77D26B@chi2k3ms01.columbuschildrens.net> Cardinal has them, Cat# M6306-10; come in packs of 10 or case of 100 Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Columbus Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 houstonr@chi.osu.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, May 10, 2007 4:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide files for binders Does anyone know where I can purchase plastic "pages" that hold glass microscope slides and have three holes in the page to put it into a binder? Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: This e-mail message, from Children's Hospital, Columbus, Ohio, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes pursuant to Children's Hospital's confidentiality policies. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From ryeo <@t> wchosp.org Fri May 11 07:54:05 2007 From: ryeo <@t> wchosp.org (Richard Yeo) Date: Fri May 11 07:52:53 2007 Subject: FW: [Histonet] timer calibration Message-ID: -----Original Message----- From: Richard Yeo Sent: Friday, May 11, 2007 8:51 AM To: 'histonet-bounces@lists.utsouthwestern.edu' Subject: FW: [Histonet] timer calibration Just buy the certified timers from Thermo/Fisher. They are certified for two years. When they expire toss them and buy new ones. We have been doing this for quite some time and have never been dinged by CAP. Cat. # 14-649-17 $25.56 each. Richard E Yeo Histology Supervisor Wooster Community Hospital Wooster Ohio (330)263-8563 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margiotta, Michele Sent: Tuesday, May 08, 2007 3:42 PM To: histonet@pathology.swmed.edu Subject: [Histonet] timer calibration Hi All, Does anyone have a procedure for calibrating timers? We just had an inspection and got cited because our timers were not calibrated. Any info would be appreciated! Michele This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jmahoney <@t> alegent.org Fri May 11 08:40:32 2007 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Fri May 11 08:40:54 2007 Subject: [Histonet] timer calibration In-Reply-To: <008f01c79763$d698e7d0$d49eae18@yourxhtr8hvc4p> References: <113979.14134.qm@web61212.mail.yahoo.com> <00c801c79639$bc0a91a0$d49eae18@yourxhtr8hvc4p> <002d01c79259$22348380$6500a8c0@mainbox> <001301c79333$3cb3a5d0$7701a80a@Ford> <008f01c79763$d698e7d0$d49eae18@yourxhtr8hvc4p> Message-ID: <46442C000200003C0000DA50@gwia.alegent.org> That would all depend on the hydrogen ion concentration, temeperature, penetration, osmolality, concentration and duration. Jan, Omaha >>> "Joe Nocito" 05/15/2007 9:42 PM >>> If I move to Arizona, will my timer be fixed? ----- Original Message ----- From: "Ford Royer" To: Sent: Thursday, May 10, 2007 1:44 PM Subject: RE: [Histonet] timer calibration It's really not the Timer or the Inspector that is off. What screwed up the whole aspect of "Time" and "Timers" was the advent of Day Light Savings Time... which was, of course, adapted from an ancient practice by an early Norwegian bachelor farmers who settled in Minnesota. One night he noticed that the blanket on his bed was too short to cover his bare feet when he was sleeping. So he cut six inches off the top of the blanket and sewed it on to the bottom. Problem solved. But I digress... Ford M. Royer, MT(ASCP) Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL: 612-839-1046 Phone: 763-542-8725 Fax: 763-546-4830 eMail: clinicallab@minnesotamedical.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bryan Hewlett Sent: Wednesday, May 09, 2007 11:43 AM To: Joe Nocito; Rene J Buesa; Margiotta, Michele; histonet@pathology.swmed.edu Subject: Re: [Histonet] timer calibration Joe, You are talking fundamental philosophical differences here. The inspectors are obviously Newtonian realists, while you're leaning more to the Leibnitz/Kant view. They are going to win! Bryan ----- Original Message ----- From: "Joe Nocito" To: "Rene J Buesa" ; "Margiotta, Michele" ; Sent: Monday, May 14, 2007 11:08 AM Subject: Re: [Histonet] timer calibration I'm sorry am I the only one that thinks calibrating timers is stupid. I mean how many histology procedures are so time sensitive that the timers have to be calibrated? Let's face it. I have had techs sit there and watch the clock and rinse as soon as the timer goes off. And I've had techs wait for the timer to go off , then mosey over and rinse. Both stains worked. I guess I'm just not anal enough. JTT ----- Original Message ----- From: "Rene J Buesa" To: "Margiotta, Michele" ; Sent: Tuesday, May 08, 2007 3:39 PM Subject: Re: [Histonet] timer calibration > Try any local watch repair store. They have to calibrate their repaired > watches so it is very likely they can help you. > Ren? J. > > "Margiotta, Michele" wrote: > Hi All, > > Does anyone have a procedure for calibrating timers? We just had an > inspection and got cited because our timers were not calibrated. Any info > would be appreciated! > > Michele > > > > This e-mail and any files transmitted with it are confidential and are > intended > solely for the use of the individual or entity to which they are > addressed. > This communication may contain material protected by the attorney-client > privilege. If you are not the intended recipient or the person responsible > for > delivering the e-mail to the intended recipient, be advised that you have > received > this e-mail in error and that any use, dissemination, forwarding, > printing, > or copying of this e-mail is strictly prohibited. If you have received > this e-mail in > error, please immediately notify the sender via return e-mail or call > Brookhaven Memorial Hospital Medical Center at (631) 654-7282. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Ahhh...imagining that irresistible "new car" smell? > Check outnew cars at Yahoo! Autos. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shu-cheng.chen <@t> spcorp.com Fri May 11 08:52:10 2007 From: shu-cheng.chen <@t> spcorp.com (Chen, Shu-Cheng) Date: Fri May 11 08:52:38 2007 Subject: [Histonet] Ki16 and BDCA Message-ID: <886D951F4246D94DAF28C82DC102DDD0024F246F@KENMSG20.us.schp.com> Hi, I have a question about antibodies for Ki16 (cytokeratine16) and BDCA. Is there any antibody that works with paraffin human tissues? Any information you can share, even negative experience, is very much appreciated. Thanks, Shu-Cheng ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From dusko.trajkovic <@t> pfizer.com Fri May 11 09:20:18 2007 From: dusko.trajkovic <@t> pfizer.com (Trajkovic, Dusko) Date: Fri May 11 09:20:47 2007 Subject: [Histonet] PCV-2 antibody In-Reply-To: Message-ID: <3AD0BD3142459B4E9B12CBEAFF2B89B2046A6D48@lajamrexm01.amer.pfizer.com> Does anyone have any information on PCV-2 (Porcine Circovirus Type 2) antibody? ANY information is greatly appreciated. Thank you and have a great weekend. To all Histology Mom's. Happy mothers day!!! Dusko Trajkovic ---------------------------------------------------------------------- LEGAL NOTICE Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this E-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately. From anh2006 <@t> med.cornell.edu Fri May 11 09:25:14 2007 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Fri May 11 09:25:37 2007 Subject: [Histonet] Ki 67 antibody Message-ID: Add another vote for DAKO, clone Tec3, it works very well for me on paraffin sections (though I have not tried the others ... so who knows). >Date: Fri, 11 May 2007 10:53:25 +0100 >From: "Edwards, R.E." >Subject: [Histonet] Ki 67 antibody of choice feedback. >Sender: histonet-bounces@lists.utsouthwestern.edu > > > >Recommendations:- > > BD Pharmingen 2 > > Labvision 6 > > DAKO 2 > > Many thanks for your inputs.......... > -- From shive003 <@t> umn.edu Fri May 11 09:38:37 2007 From: shive003 <@t> umn.edu (Jan Shivers) Date: Fri May 11 09:38:57 2007 Subject: [Histonet] PCV-2 antibody References: <3AD0BD3142459B4E9B12CBEAFF2B89B2046A6D48@lajamrexm01.amer.pfizer.com> Message-ID: <005a01c793da$0faf2a80$a1065486@auxs.umn.edu> I buy my rabbit polyclonal from: Iowa State University Veterinary Diagnostic Lab Ames, IA 515-294-1950 isuvdlv@iastate.edu www.vdpam.iastate.edu 1:1000 with Proteinase K enzyme digestion pretreatment. I buy my mouse monoclonal from: Rural Technologies, Inc. Brookings, SD 605-692-6953 info@ruraltechinc.com www.ruraltechinc.com 1:500 with Proteinase K enzyme digestion pretreatment. Good luck, Jan Shivers U of MN Veterinary Diagnostic Lab St. Paul, MN ----- Original Message ----- From: "Trajkovic, Dusko" To: Sent: Friday, May 11, 2007 9:20 AM Subject: [Histonet] PCV-2 antibody Does anyone have any information on PCV-2 (Porcine Circovirus Type 2) antibody? ANY information is greatly appreciated. Thank you and have a great weekend. To all Histology Mom's. Happy mothers day!!! Dusko Trajkovic ---------------------------------------------------------------------- LEGAL NOTICE Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this E-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Fri May 11 10:27:10 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Fri May 11 10:27:18 2007 Subject: [Histonet] Ohio Position In-Reply-To: <7E3ACD48BA6E26408F3188FBF08693F78D3B0B@titansbs1.corp.titanmed.com> References: <71437982F5B13A4D9A5B2669BDB89EE4023E3590@ISS-CL-EX-V1.soton.ac.uk> <7E3ACD48BA6E26408F3188FBF08693F78D3B0B@titansbs1.corp.titanmed.com> Message-ID: Has anyone successfully tried the Ohio position?. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of April Sachau Sent: 10 May 2007 15:27 To: Martin S.; histonet@lists.utsouthwestern.edu Subject: [Histonet] Ohio Position Hi Histo-netters! I am working with a hospital in Ohio that is in need of an ASCP certified Histologist for a temporary contract. They tentatively need someone the first week of June. Day shift/40 hours a week. Please reply if you are interested or if you know someone interested. Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Anthony.Gatt <@t> jefferson.edu Fri May 11 11:16:12 2007 From: Anthony.Gatt <@t> jefferson.edu (Anthony Gatt) Date: Fri May 11 11:10:48 2007 Subject: [Histonet] (no subject) Message-ID: <20070511121612.AHC52584@logan.jefferson.edu> Hello, I am currently sectioning drosophila heads and am tearing up the paraffin. Without the heads, I get beautiful ribbons so I suspect it is the sample itself. I am using a tissue processor with the following protocol after an o/n fix in 4% PFA. 15m -alcoholic formalin (x2) 30m -95% EtOH (x2) 30m -100% EtOH (x2) 30m -Histoclear (x3) 30m -Paraffin (x2) 45m -Paraffin Embed immediately in paraffin, cold block 30m, section next day. I am new to this an would greatly appreciate any help. Thank you, Anthony From rjbuesa <@t> yahoo.com Fri May 11 11:15:23 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri May 11 11:15:29 2007 Subject: [Histonet] (no subject) In-Reply-To: <20070511121612.AHC52584@logan.jefferson.edu> Message-ID: <248827.34387.qm@web61214.mail.yahoo.com> Your problem resides in the fact that you have to "soften" the chitin before processing the heads, otherwise the chitin cannot be infiltrated by the paraffin, causing the tears you are having. Soften the chitin first and process afterwards. Ren? J. Anthony Gatt wrote: Hello, I am currently sectioning drosophila heads and am tearing up the paraffin. Without the heads, I get beautiful ribbons so I suspect it is the sample itself. I am using a tissue processor with the following protocol after an o/n fix in 4% PFA. 15m -alcoholic formalin (x2) 30m -95% EtOH (x2) 30m -100% EtOH (x2) 30m -Histoclear (x3) 30m -Paraffin (x2) 45m -Paraffin Embed immediately in paraffin, cold block 30m, section next day. I am new to this an would greatly appreciate any help. Thank you, Anthony _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get the free Yahoo! toolbar and rest assured with the added security of spyware protection. From Anthony.Gatt <@t> jefferson.edu Fri May 11 11:35:37 2007 From: Anthony.Gatt <@t> jefferson.edu (Anthony Gatt) Date: Fri May 11 11:30:33 2007 Subject: [Histonet] drosophila sectioning Message-ID: <20070511123537.AHC53634@logan.jefferson.edu> Thanks, Rene. Any advice on a softening agent and where it would fit into the protocol? ---- Original message ---- >Date: Fri, 11 May 2007 09:15:23 -0700 (PDT) >From: Rene J Buesa >Subject: Re: [Histonet] (no subject) >To: Anthony.Gatt@jefferson.edu, "histonet@lists.utsouthwestern.edu" > > Your problem resides in the fact that you have to > "soften" the chitin before processing the heads, > otherwise the chitin cannot be infiltrated by the > paraffin, causing the tears you are having. > Soften the chitin first and process afterwards. > Ren? J. > > Anthony Gatt wrote: > > Hello, I am currently sectioning drosophila heads > and am tearing up the paraffin. Without the heads, > I get beautiful ribbons so I suspect it is the > sample itself. I am using a tissue processor with > the following protocol after an o/n fix in 4% PFA. > > 15m -alcoholic formalin (x2) > 30m -95% EtOH (x2) > 30m -100% EtOH (x2) > 30m -Histoclear (x3) > 30m -Paraffin (x2) > 45m -Paraffin > > Embed immediately in paraffin, cold block 30m, > section next day. > > I am new to this an would greatly appreciate any > help. > > Thank you, > > Anthony > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------------------------ > > Get the free Yahoo! toolbar and rest assured with > the added security of spyware protection. From pvalente <@t> sbcglobal.net Fri May 11 11:35:53 2007 From: pvalente <@t> sbcglobal.net (Patricia Valente) Date: Fri May 11 11:35:58 2007 Subject: [Histonet] Time calibration Message-ID: <896031.93070.qm@web81705.mail.mud.yahoo.com> yet another thought on this thread!! My cell phone has a timer set to the world clock perhaps I could expense my cell phone bill!! Pat Valente From gcallis <@t> montana.edu Fri May 11 11:36:57 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri May 11 11:37:04 2007 Subject: [Histonet] Sorry, forgot to send to Histonet - Softening chitin - Drosophila heads) Message-ID: <6.0.0.22.1.20070511103549.01b24d18@gemini.msu.montana.edu> >Rene is correct with this suggestion. > >One way to soften the chitin is to soak the totally fixed insect in 4% >phenol, alcoholic solution for 24 hours. Beware, phenol is toxic. Make >it up by melting the phenol, add 4 ml of this to 96 ml of 80% >alcohol). Phil Oshel provided a processing schedule for small insects, I >have attached that to Anthony privately. > >At 10:15 AM 5/11/2007, you wrote: >>Your problem resides in the fact that you have to "soften" the chitin >>before processing the heads, otherwise the chitin cannot be infiltrated >>by the paraffin, causing the tears you are having. >> Soften the chitin first and process afterwards. >> Ren? J. Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 From Allison_Scott <@t> hchd.tmc.edu Fri May 11 11:42:35 2007 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Fri May 11 11:42:57 2007 Subject: [Histonet] FW: H&E Issues Message-ID: <1872B4A455B7974391609AD8034C79FC06715C@LBEXCH01.hchd.local> > -----Original Message----- > From: Scott, Allison D > Sent: Friday, May 11, 2007 11:31 AM > To: 'histonet@lists.utsouthwester.edu' > Subject: H&E Issues > > We are having a problem with our H&E slides. The problem just started this week. One of our pathologist said that the stain was too dark. After looking at the solutions in the stainer, I determined that the bluing solution had not been changed as it should have been and I changed it. I also changed the hematoxylin and the clarifier. The next day the stain was fine. Now the pathologist is saying that the sections look air dried or cooked. I checked the temperatures on the waterbaths, embedding stations, vip processor and the stainer. We have a Shandon Gemini stainer. This is a mixture of GI, Skin,Gyn, and other biopsies. The surgicals are not affected. The only other thing I can think of is the coverslipper. We have a Leica automatic glass coverslipper. Any help in this situation would be greatly appreciated. Happy Friday > > Allison Scott > Histology Supervisor > LBJ Hospital > Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From kbradshaw <@t> lcpath.com Fri May 11 11:50:35 2007 From: kbradshaw <@t> lcpath.com (Kari Bradshaw) Date: Fri May 11 11:50:39 2007 Subject: [Histonet] RE: H&E Issues In-Reply-To: <1872B4A455B7974391609AD8034C79FC06715C@LBEXCH01.hchd.local> Message-ID: What type of paraffin are you using? We had similar problems awhile back. The paraffin we had used for many years had a change in where is was being manufactured, which resulted in somewhat of a different recipe. Call me and I can fill you in on what we were using and how we fixed the problem. Kari Bradshaw Anatomic Pathology Manager Lower Columbia Pathologists 1217 14th Ave Longview, WA 98632 360.425.5620 kbradshaw@lcpath.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Friday, May 11, 2007 9:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: H&E Issues > -----Original Message----- > From: Scott, Allison D > Sent: Friday, May 11, 2007 11:31 AM > To: 'histonet@lists.utsouthwester.edu' > Subject: H&E Issues > > We are having a problem with our H&E slides. The problem just started this week. One of our pathologist said that the stain was too dark. After looking at the solutions in the stainer, I determined that the bluing solution had not been changed as it should have been and I changed it. I also changed the hematoxylin and the clarifier. The next day the stain was fine. Now the pathologist is saying that the sections look air dried or cooked. I checked the temperatures on the waterbaths, embedding stations, vip processor and the stainer. We have a Shandon Gemini stainer. This is a mixture of GI, Skin,Gyn, and other biopsies. The surgicals are not affected. The only other thing I can think of is the coverslipper. We have a Leica automatic glass coverslipper. Any help in this situation would be greatly appreciated. Happy Friday > > Allison Scott > Histology Supervisor > LBJ Hospital > Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From opiecurt <@t> yahoo.com Fri May 11 11:53:01 2007 From: opiecurt <@t> yahoo.com (curt tague) Date: Fri May 11 12:19:44 2007 Subject: [Histonet] looking for experienced tech in southern CA. that was southern.... by the BEACH. Message-ID: <90680.44138.qm@web81612.mail.mud.yahoo.com> the title says it all, i'm needing tech's to help embed and cut just over 500 blocks/day. hospital and derm specimens with a few routine specials. curt --------------------------------- Never miss an email again! Yahoo! Toolbar alerts you the instant new Mail arrives. Check it out. From ROrr <@t> enh.org Fri May 11 12:42:07 2007 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Fri May 11 12:42:12 2007 Subject: [Histonet] Ohio Position Message-ID: Hmmmm when I saw this posting, the first thing I thought of was the game of Twister..... HAPPY FRIDAY!!!!!! Becky Message: 3 Date: Fri, 11 May 2007 16:27:10 +0100 From: "Edwards, R.E." Subject: RE: [Histonet] Ohio Position To: Has anyone successfully tried the Ohio position?. -----Original Message----- Sent: 10 May 2007 15:27 To: Martin S.; histonet@lists.utsouthwestern.edu Subject: [Histonet] Ohio Position Hi Histo-netters! I am working with a hospital in Ohio that is in need of an ASCP certified Histologist for a temporary contract. They tentatively need someone the first week of June. Day shift/40 hours a week. Please reply if you are interested or if you know someone interested. Thanks! Becky Orr CLA,HT(ASCP)QIHC Assistant Manager, Anatomic Pathology Evanston Northwestern Healthcare 847-570-2763 -----Original Message----- From mickie25 <@t> netzero.net Fri May 11 13:10:50 2007 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Fri May 11 13:12:57 2007 Subject: [PHISH] FW: [Histonet] timer calibration In-Reply-To: References: Message-ID: Hi Everyone, I have been reading the correspondence about timers for a few days. It never ceases to amaze me how a bureaucracy can create rules that have to real merit, requiring everyone to jump through hoops, (occasionally burning hoops!) to no real purpose. After 35 years, I still have yet to see (even with IHC stains) how a 'certified' timer adds anything at all to the accuracy of a stain. Isn't it the training and experience of the histotechnologist that determines how useful and readable a special stain is? Lee Luna, Desna Sheehan and a few others would turn over in their graves! Now that I have let off steam about this, I hope everyone has a great weekend! By the way, if there are any techs out their who would like to learn how fun Mohs histology is, let me know. I have doctors looking for qualified people. Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Yeo Sent: Friday, May 11, 2007 5:54 AM To: histonet@lists.utsouthwestern.edu Subject: [PHISH] FW: [Histonet] timer calibration -----Original Message----- From: Richard Yeo Sent: Friday, May 11, 2007 8:51 AM To: 'histonet-bounces@lists.utsouthwestern.edu' Subject: FW: [Histonet] timer calibration Just buy the certified timers from Thermo/Fisher. They are certified for two years. When they expire toss them and buy new ones. We have been doing this for quite some time and have never been dinged by CAP. Cat. # 14-649-17 $25.56 each. Richard E Yeo Histology Supervisor Wooster Community Hospital Wooster Ohio (330)263-8563 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margiotta, Michele Sent: Tuesday, May 08, 2007 3:42 PM To: histonet@pathology.swmed.edu Subject: [Histonet] timer calibration Hi All, Does anyone have a procedure for calibrating timers? We just had an inspection and got cited because our timers were not calibrated. Any info would be appreciated! Michele This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Anthony.Gatt <@t> jefferson.edu Fri May 11 13:25:38 2007 From: Anthony.Gatt <@t> jefferson.edu (Anthony Gatt) Date: Fri May 11 13:20:17 2007 Subject: [Histonet] drosophila sectioning Message-ID: <20070511142538.AHC58914@logan.jefferson.edu> thanks, James. Just curious, what is T&S under the agitation? I only have the options for "on" or "off" on my processor. Also do you use the same paraffin for embedding? ---- Original message ---- >Date: Fri, 11 May 2007 09:59:07 -0700 >From: "James Watson" >Subject: RE: [Histonet] drosophila sectioning >To: , "Rene J Buesa" , > > > > Anthony, > > > > This is the processing schedule that I have used on > drosophila heads,? note that I use 5% Glycerin in > the absolute alcohols.? The fixation was formalin > or 4% PFA with no softening agent used other than > the? glycerin. > > > > > > > > Drosophila Processing Schedule > >+--------------------------------------------------------+ >|Drosophila Tissue Processing Schedule | >| | >|+------------------------------------------------------+| >||Station|Reagent |Temperature|Vacuum|Time|Drain|Agitate|| >|| | | | | |Time | || >||-------+--------+-----------+------+----+-----+-------|| >|| 1 |70% ETOH| Ambient | OFF |0:15| 30 |Off || >||-------+--------+-----------+------+----+-----+-------|| >|| 2 |80% ETOH| Ambient |Vacuum|0:20| 30 |Stirred|| >||-------+--------+-----------+------+----+-----+-------|| >|| 3 |95% ETOH| Ambient |Vacuum|0:15| 30 |Stirred|| >||-------+--------+-----------+------+----+-----+-------|| >|| 4 |95% ETOH| Ambient |Vacuum|0:20| 45 |Stirred|| >||-------+--------+-----------+------+----+-----+-------|| >|| 5 | 5% | Ambient |Vacuum|0:15| 30 |Stirred|| >|| |Glycerin| | | | | || >|| | in | | | | | || >|| | | | | | | || >|| | 100% | | | | | || >|| | ETOH | | | | | || >||-------+--------+-----------+------+----+-----+-------|| >|| 6 | 5% | Ambient |Vacuum|0:20| 30 | T&S || >|| |Glycerin| | | | | || >|| | in | | | | | || >|| | | | | | | || >|| | 100% | | | | | || >|| | ETOH | | | | | || >||-------+--------+-----------+------+----+-----+-------|| >|| 7 | 5% | Ambient |Vacuum|0:30| 100 | T&S || >|| |Glycerin| | | | | || >|| | in | | | | | || >|| | | | | | | || >|| | 100% | | | | | || >|| | ETOH | | | | | || >||-------+--------+-----------+------+----+-----+-------|| >|| 8 | Xylene | Ambient |Vacuum|0:15| 45 |Stirred|| >||-------+--------+-----------+------+----+-----+-------|| >|| 9 |Xylene | Ambient |Vacuum|0:20| 45 |T&S || >||-------+--------+-----------+------+----+-----+-------|| >|| 10 | Xylene | Ambient |Vacuum|0:30| 120 | T&S || >||-------+--------+-----------+------+----+-----+-------|| >|| 11 |Paraffin| 60 |Vacuum|0:15| 120 |Stirred|| >||-------+--------+-----------+------+----+-----+-------|| >|| 12 |Paraffin| 60 |Vacuum|0:20| 120 |Stirred|| >||-------+--------+-----------+------+----+-----+-------|| >|| 13 |Paraffin| 60 |Vacuum|0:20| 120 |Stirred|| >||-------+--------+-----------+------+----+-----+-------|| >|| 14 |Paraffin| 60 |Vacuum|0:30| 120 |Stirred|| >|+------------------------------------------------------+| >| | >| | >| | >| | >+--------------------------------------------------------+ > > > > > > > > > > > > James Watson? HT? ASCP > > Facilities Manager of Histology > > GNF,? Genomics Institute of the Novartis Research > foundation > > jwatson@gnf.org > > 858-332-4647 > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Anthony Gatt > Sent: Friday, May 11, 2007 9:36 AM > To: Rene J Buesa; Anthony.Gatt@jefferson.edu; > histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] drosophila sectioning > > > > Thanks, Rene.? Any advice on a softening agent and > where it would fit into the protocol? > > > > ---- Original message ---- > > >Date: Fri, 11 May 2007 09:15:23 -0700 (PDT) > > >From: Rene J Buesa ? > > >Subject: Re: [Histonet] (no subject)? > > >To: Anthony.Gatt@jefferson.edu, > "histonet@lists.utsouthwestern.edu" > > > > > > >?? Your problem resides in the fact that you have > to > > >?? "soften" the chitin before processing the > heads, > > >?? otherwise the chitin cannot be infiltrated by > the > > >?? paraffin, causing the tears you are having. > > >?? Soften the chitin first and process > afterwards. > > >?? Ren? J. > > > > > >?? Anthony Gatt > wrote: > > > > > >???? Hello, I am currently sectioning > drosophila heads > > >???? and am tearing up the paraffin. Without > the heads, > > >???? I get beautiful ribbons so I suspect it is > the > > >???? sample itself. I am using a tissue > processor with > > >???? the following protocol after an o/n fix in > 4% PFA. > > > > > >???? 15m -alcoholic formalin (x2) > > >???? 30m -95% EtOH (x2) > > > ????30m -100% EtOH (x2) > > >???? 30m -Histoclear (x3) > > >???? 30m -Paraffin (x2) > > >???? 45m -Paraffin > > > > > >???? Embed immediately in paraffin, cold block > 30m, > > >???? section next day. > > > > > >???? I am new to this an would greatly > appreciate any > > >???? help. > > > > > >???? Thank you, > > > > > >???? Anthony > > > > > >???? > _______________________________________________ > > >???? Histonet mailing list > > >???? Histonet@lists.utsouthwestern.edu > > >???? > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > > >???? > ------------------------------------------------ > > > > > >?? Get the free Yahoo! toolbar and rest assured > with > > >?? the added security of spyware protection. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Fri May 11 13:43:27 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri May 11 13:43:33 2007 Subject: [Histonet] Ohio Position In-Reply-To: Message-ID: <001601c793fc$4362ab50$d00f7ca5@lurie.northwestern.edu> Ooh Becky, can I play too? We can do Chicago twister!!!! Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Orr, Rebecca Sent: Friday, May 11, 2007 11:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ohio Position Hmmmm when I saw this posting, the first thing I thought of was the game of Twister..... HAPPY FRIDAY!!!!!! Becky Message: 3 Date: Fri, 11 May 2007 16:27:10 +0100 From: "Edwards, R.E." Subject: RE: [Histonet] Ohio Position To: Has anyone successfully tried the Ohio position?. -----Original Message----- Sent: 10 May 2007 15:27 To: Martin S.; histonet@lists.utsouthwestern.edu Subject: [Histonet] Ohio Position Hi Histo-netters! I am working with a hospital in Ohio that is in need of an ASCP certified Histologist for a temporary contract. They tentatively need someone the first week of June. Day shift/40 hours a week. Please reply if you are interested or if you know someone interested. Thanks! Becky Orr CLA,HT(ASCP)QIHC Assistant Manager, Anatomic Pathology Evanston Northwestern Healthcare 847-570-2763 -----Original Message----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jjohnson <@t> crispregional.org Fri May 11 13:44:58 2007 From: jjohnson <@t> crispregional.org (Jennifer Johnson) Date: Fri May 11 13:47:52 2007 Subject: [Histonet] Salary Information Message-ID: <000001c793fc$7a13fb40$2082010a@main.crispregional.org> Can any of you tell me what a HTL (ASCP) with a BS in Biology and six years of experience should be making in or around South Georgia? I am having a job re-evaluation for a status change and want to be sure I am being fairly paid. Any help is appreciated. Thanks, Jennifer From tkngflght <@t> yahoo.com Fri May 11 13:40:03 2007 From: tkngflght <@t> yahoo.com (Cheryl Kerry) Date: Fri May 11 13:48:51 2007 Subject: [Histonet] Open temp and temp-to-permanent histology positions In-Reply-To: <20070511142538.AHC58914@logan.jefferson.edu> References: <20070511142538.AHC58914@logan.jefferson.edu> Message-ID: <001101c793fb$caa71390$6501a8c0@FSDESKTOP> Hi All- I hope this job table (below) comes through okay. Please-if you are even REMOTELY curious-call me. I've been a tech since 1982 and have temped on and off since 1993-I'll tell you the good and the bad about temping and you can make up your own mind. I've benched one of these labs, and have had temps and staffed permanents in the rest. All are well-managed and happy places to be but need help as they are GROWING! We have a separate list of permanent openings that I'll post in just a bit- We'll be working this weekend to fill these-800.756.3309 is the easiest way to talk with me directly. Cheryl Kerry, HT(ASCP) Full Staff Inc. 281.852.9457 800.756.3309 Sign up for the FREE newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. Location Position Shift Start date Duration Ohio bench 3rd ASAP 13 weeks Ohio bench 3rd 30-May 13 weeks Connecticut bench 2nd ASAP 13 weeks Connecticut IHC 1st ASAP 4-6 weeks Connecticut bench 2nd ASAP 13 weeks New Jersey Mohs 1st ASAP 6-8 weeks Connecticut Supervisor 2nd ASAP 13 weeks From rjbuesa <@t> yahoo.com Fri May 11 13:45:01 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri May 11 13:51:46 2007 Subject: [Histonet] drosophila sectioning In-Reply-To: <20070511123537.AHC53634@logan.jefferson.edu> Message-ID: <212532.89950.qm@web61211.mail.yahoo.com> Anthony: Hennings (1900) described a method to soften chitin, but I don't recommend it (too many toxic components). Slifer and King (1933) developed another method: Dist. water (30 mL) + 95% Ethanol (80 mL) + phenol (4 mL), but Roonwall (1935) reduces the amount of phenol to 1-2 mL These phenol containing solutions are good to soften chitin and, once is soft, will be readily infiltrated with the paraffin. The insects should be fixed first. Ren? J. Anthony Gatt wrote: Thanks, Rene. Any advice on a softening agent and where it would fit into the protocol? ---- Original message ---- >Date: Fri, 11 May 2007 09:15:23 -0700 (PDT) >From: Rene J Buesa >Subject: Re: [Histonet] (no subject) >To: Anthony.Gatt@jefferson.edu, "histonet@lists.utsouthwestern.edu" > > Your problem resides in the fact that you have to > "soften" the chitin before processing the heads, > otherwise the chitin cannot be infiltrated by the > paraffin, causing the tears you are having. > Soften the chitin first and process afterwards. > Ren? J. > > Anthony Gatt wrote: > > Hello, I am currently sectioning drosophila heads > and am tearing up the paraffin. Without the heads, > I get beautiful ribbons so I suspect it is the > sample itself. I am using a tissue processor with > the following protocol after an o/n fix in 4% PFA. > > 15m -alcoholic formalin (x2) > 30m -95% EtOH (x2) > 30m -100% EtOH (x2) > 30m -Histoclear (x3) > 30m -Paraffin (x2) > 45m -Paraffin > > Embed immediately in paraffin, cold block 30m, > section next day. > > I am new to this an would greatly appreciate any > help. > > Thank you, > > Anthony > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------------------------ > > Get the free Yahoo! toolbar and rest assured with > the added security of spyware protection. --------------------------------- Be a better Globetrotter. Get better travel answers from someone who knows. Yahoo! Answers - Check it out. From jqb7 <@t> cdc.gov Fri May 11 13:55:51 2007 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Fri May 11 13:56:54 2007 Subject: [Histonet] Salary Information References: <000001c793fc$7a13fb40$2082010a@main.crispregional.org> Message-ID: This should help. http://www.nsh.org/organizations.php3?action=printContentTypeHome&orgid= 111&typeID=1197 Jeanine Bartlett Infectious Disease Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Johnson Sent: Friday, May 11, 2007 2:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Salary Information Can any of you tell me what a HTL (ASCP) with a BS in Biology and six years of experience should be making in or around South Georgia? I am having a job re-evaluation for a status change and want to be sure I am being fairly paid. Any help is appreciated. Thanks, Jennifer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri May 11 14:01:16 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri May 11 14:03:39 2007 Subject: [Histonet] Salary Information In-Reply-To: <000001c793fc$7a13fb40$2082010a@main.crispregional.org> Message-ID: <294276.86861.qm@web61214.mail.yahoo.com> Depends on the tasks: for bench positions, between $22-$25 if it includes HC and IHC, between $25-$30 if to do ISH, FISH, or TEM: more than $30 (negotiable). Ren? J. Jennifer Johnson wrote: Can any of you tell me what a HTL (ASCP) with a BS in Biology and six years of experience should be making in or around South Georgia? I am having a job re-evaluation for a status change and want to be sure I am being fairly paid. Any help is appreciated. Thanks, Jennifer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ready for the edge of your seat? Check out tonight's top picks on Yahoo! TV. From trinimaican2501 <@t> yahoo.com Fri May 11 15:15:58 2007 From: trinimaican2501 <@t> yahoo.com (I-sanna Gibbons) Date: Fri May 11 15:18:24 2007 Subject: [Histonet] chattering Message-ID: <644100.81238.qm@web50301.mail.re2.yahoo.com> Hi everyone, my problem is more chattering of the bat brain tissue rather than cracking. Does chattering occur easily with the bat brain? Waht are the causes of this chattering and how can I prevent it? Thanks I-sanna I-sanna Gibbons, DVM Veterinary Anatomy School of Veterinary Medicine The University of the West Indies Trinidad and Tobago, W.I. --------------------------------- Park yourself in front of a world of choices in alternative vehicles. Visit the Yahoo! Auto Green Center. From mpence <@t> grhs.net Fri May 11 15:33:00 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Fri May 11 15:35:03 2007 Subject: [Histonet] chattering In-Reply-To: <644100.81238.qm@web50301.mail.re2.yahoo.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C5C9@IS-E2K3.grhs.net> Chatter can be from a number of reasons. Loose knife blade. Angle of knife not correct. Too "dry" of tissue. (over fixed) Speed the tissue is cut across the blade. The material the tissue is embedded in. The size of the tissue being cut. C.L. Sturkey, Inc. Lebanon, PA. USA put out a paper about microtomy trouble-shooting guide. See if you can locate that for reference. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of I-sanna Gibbons Sent: Friday, May 11, 2007 3:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] chattering Hi everyone, my problem is more chattering of the bat brain tissue rather than cracking. Does chattering occur easily with the bat brain? Waht are the causes of this chattering and how can I prevent it? Thanks I-sanna I-sanna Gibbons, DVM Veterinary Anatomy School of Veterinary Medicine The University of the West Indies Trinidad and Tobago, W.I. --------------------------------- Park yourself in front of a world of choices in alternative vehicles. Visit the Yahoo! Auto Green Center. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ander093 <@t> tc.umn.edu Fri May 11 15:49:45 2007 From: ander093 <@t> tc.umn.edu (LuAnn Anderson) Date: Fri May 11 15:52:06 2007 Subject: [Histonet] chattering In-Reply-To: <661949901A768E4F9CC16D8AF8F2838CA1C5C9@IS-E2K3.grhs.net> References: <644100.81238.qm@web50301.mail.re2.yahoo.com> <661949901A768E4F9CC16D8AF8F2838CA1C5C9@IS-E2K3.grhs.net> Message-ID: I would soak the blocks, then make sure everything on the microtome is tightened down well (blade, block clamp etc.) and see if that will work. At 03:33 PM 5/11/2007, Mike Pence wrote: >Chatter can be from a number of reasons. > >Loose knife blade. Angle of knife not correct. Too "dry" of tissue. >(over fixed) Speed the tissue is cut across the blade. The material >the tissue is embedded in. The size of the tissue being cut. C.L. >Sturkey, Inc. Lebanon, PA. USA put out a paper about microtomy >trouble-shooting guide. See if you can locate that for reference. > >Mike > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of I-sanna >Gibbons >Sent: Friday, May 11, 2007 3:16 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] chattering > > >Hi everyone, my problem is more chattering of the bat brain tissue >rather than cracking. Does chattering occur easily with the bat brain? >Waht are the causes of this chattering and how can I prevent it? > Thanks > I-sanna > > I-sanna Gibbons, DVM > Veterinary Anatomy > School of Veterinary Medicine > The University of the West Indies > Trinidad and Tobago, W.I. > > >--------------------------------- >Park yourself in front of a world of choices in alternative vehicles. >Visit the Yahoo! Auto Green Center. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sonyprasad <@t> hotmail.com Fri May 11 15:55:45 2007 From: sonyprasad <@t> hotmail.com (sony prasad) Date: Fri May 11 15:55:53 2007 Subject: [Histonet] KI67 staining Message-ID: I'll second that, DAKO cloetech 3 works really well on FFPE sections. Very crisp staining and no background at all. I use citrate buffer with tween20 (microvave in 5min pulses for 25min). _________________________________________________________________ Sign in and get updated on all the action from Formula One http://content.msn.co.in/Sports/FormulaOne/Default From CIngles <@t> uwhealth.org Fri May 11 15:59:06 2007 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Fri May 11 15:59:14 2007 Subject: [Histonet] RE: timer calibration References: Message-ID: <08A0A863637F1349BBFD83A96B27A50A120040@uwhis-xchng3.uwhis.hosp.wisc.edu> Does that mean that WE need to be calibrated yearly now? What would the standards be? Happy Friday, and upcoming Mother's Day. I'm going fishing! Then I get to 'gross' the fish for the frying pan. Mmmm. Don't worry, I'll watch my Mercury intake. I don't need to loose mental functioning any faster than it has been happening lately. Maybe I'm just allergic to my pathologists. Could I get workman's comp. for that? Sorry about the rambling. Claire Hi Everyone, I have been reading the correspondence about timers for a few days. It never ceases to amaze me how a bureaucracy can create rules that have to real merit, requiring everyone to jump through hoops, (occasionally burning hoops!) to no real purpose. After 35 years, I still have yet to see (even with IHC stains) how a 'certified' timer adds anything at all to the accuracy of a stain. Isn't it the training and experience of the histotechnologist that determines how useful and readable a special stain is? Lee Luna, Desna Sheehan and a few others would turn over in their graves! Now that I have let off steam about this, I hope everyone has a great weekend! By the way, if there are any techs out their who would like to learn how fun Mohs histology is, let me know. I have doctors looking for qualified people. Mickie From asachau <@t> titanmed.com Fri May 11 16:08:04 2007 From: asachau <@t> titanmed.com (April Sachau) Date: Fri May 11 16:10:31 2007 Subject: [Histonet] Mother's Day In-Reply-To: <08A0A863637F1349BBFD83A96B27A50A120040@uwhis-xchng3.uwhis.hosp.wisc.edu> Message-ID: <7E3ACD48BA6E26408F3188FBF08693F78D40E5@titansbs1.corp.titanmed.com> Happy Early Mother's Day to all you Mother's out there! I hope you all have a splendid weekend!!! April Sachau Titan Medical Group Staff Supervisor Phone (866) 332-9600 Ext. 1023 Fax (402) 332-5181 asachau@titanmed.com see us on the web at www.titanmed.com From sheila_adey <@t> hotmail.com Fri May 11 20:11:36 2007 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Fri May 11 20:11:40 2007 Subject: [Histonet] chattering In-Reply-To: Message-ID: Someone on histonet mentioned a 15 second soak on your water bath, then back to the cold plate. It works wonders. Sheila Adey HT MLT Port Huron Hospital Michigan >From: LuAnn Anderson >To: "Mike Pence" ,"I-sanna Gibbons" >, >Subject: RE: [Histonet] chattering >Date: Fri, 11 May 2007 15:49:45 -0500 > >I would soak the blocks, then make sure everything on the microtome is >tightened down well (blade, block clamp etc.) and see if that will work. > > >At 03:33 PM 5/11/2007, Mike Pence wrote: >>Chatter can be from a number of reasons. >> >>Loose knife blade. Angle of knife not correct. Too "dry" of tissue. >>(over fixed) Speed the tissue is cut across the blade. The material >>the tissue is embedded in. The size of the tissue being cut. C.L. >>Sturkey, Inc. Lebanon, PA. USA put out a paper about microtomy >>trouble-shooting guide. See if you can locate that for reference. >> >>Mike >> >>-----Original Message----- >>From: histonet-bounces@lists.utsouthwestern.edu >>[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of I-sanna >>Gibbons >>Sent: Friday, May 11, 2007 3:16 PM >>To: histonet@lists.utsouthwestern.edu >>Subject: [Histonet] chattering >> >> >>Hi everyone, my problem is more chattering of the bat brain tissue >>rather than cracking. Does chattering occur easily with the bat brain? >>Waht are the causes of this chattering and how can I prevent it? >> Thanks >> I-sanna >> >> I-sanna Gibbons, DVM >> Veterinary Anatomy >> School of Veterinary Medicine >> The University of the West Indies >> Trinidad and Tobago, W.I. >> >> >>--------------------------------- >>Park yourself in front of a world of choices in alternative vehicles. >>Visit the Yahoo! Auto Green Center. >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ See Fireworks On Live Image Search http://search.live.com/images/results.aspx?q=Fireworks&mkt=en-ca&FORM=SERNEP From skitlbug <@t> yahoo.com Fri May 11 22:15:27 2007 From: skitlbug <@t> yahoo.com (skitlbug@yahoo.com) Date: Fri May 11 22:17:49 2007 Subject: [Histonet] Fixing, Sectioning, and Staining Caterpillars- Help Needed Message-ID: <628110.49130.qm@web90508.mail.mud.yahoo.com> Hi all, I'm a PhD student at the University of New Orleans interested in looking at parasitoids in small caterpillars, soybean loopers (about the size of an inch worm). The parasitoids are wasps (various species) that lay their eggs inside the parasitoid. The wasp eggs hatch and the wasp undergoes molting and development inside the caterpillar eventually forming a pupae, metamorphosing, and emerging. I'm looking to make a contact that has experience with the histology of insects that can give me some real assistance in how to process caterpillars through fixing, embedding, sectioning, staining, etc. The only thing I am interested in seeing is where the parasitoids are located in the caterpillar so I don't think I need anything too fancy in the way of special staining. My University was greatly impacted by Hurricane Katrina and I am having to set up a makeshift histology lab from the ground up. (Actually, everything in New Orleans is being done from the ground up right now.) Basically, all I have right now is a microtome, a histology catalog, and some money to get this started, but I could really use some step-by-step recipies, instructions, and advice! Any help is greatly appreciated. I'll be glad to answer any further questions about my project and thanks in advance. Very Sincerely, Sarah Brock skitlbug@yahoo.com or stemple@uno.edu --------------------------------- Ready for the edge of your seat? Check out tonight's top picks on Yahoo! TV. From Jacqueline.Malam <@t> rli.mbht.nhs.uk Mon May 14 02:57:12 2007 From: Jacqueline.Malam <@t> rli.mbht.nhs.uk (Malam Jacqueline) Date: Mon May 14 03:00:15 2007 Subject: [Histonet] RE: Ki67 Message-ID: We use Lab Vision's on the Ventana Benchmark XT - superb! Jacqui Lancaster uk DISCLAIMER: This e-mail is confidential and privileged. If you are not the intended recipient please accept our apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform postmaster@rli.mbht.nhs.uk that this message has gone astray before deleting it. Comments or opinions expressed in this email are those of their respective contributors only. The views expressed do not represent the views of the Trust, its management or employees. University Hospitals of Morecambe Bay NHS Trust is not responsible and disclaims any and all liability for the content of comments written within.Thank you for your co-operation. From ree3 <@t> leicester.ac.uk Mon May 14 03:27:19 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Mon May 14 03:27:30 2007 Subject: [Histonet] Ohio Position In-Reply-To: <001601c793fc$4362ab50$d00f7ca5@lurie.northwestern.edu> References: <001601c793fc$4362ab50$d00f7ca5@lurie.northwestern.edu> Message-ID: Twister eh?!. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: 11 May 2007 19:43 To: 'Orr, Rebecca'; histonet Subject: RE: [Histonet] Ohio Position Ooh Becky, can I play too? We can do Chicago twister!!!! Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Orr, Rebecca Sent: Friday, May 11, 2007 11:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ohio Position Hmmmm when I saw this posting, the first thing I thought of was the game of Twister..... HAPPY FRIDAY!!!!!! Becky Message: 3 Date: Fri, 11 May 2007 16:27:10 +0100 From: "Edwards, R.E." Subject: RE: [Histonet] Ohio Position To: Has anyone successfully tried the Ohio position?. -----Original Message----- Sent: 10 May 2007 15:27 To: Martin S.; histonet@lists.utsouthwestern.edu Subject: [Histonet] Ohio Position Hi Histo-netters! I am working with a hospital in Ohio that is in need of an ASCP certified Histologist for a temporary contract. They tentatively need someone the first week of June. Day shift/40 hours a week. Please reply if you are interested or if you know someone interested. Thanks! Becky Orr CLA,HT(ASCP)QIHC Assistant Manager, Anatomic Pathology Evanston Northwestern Healthcare 847-570-2763 -----Original Message----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LINDA.MARGRAF <@t> childrens.com Mon May 14 08:19:51 2007 From: LINDA.MARGRAF <@t> childrens.com (LINDA MARGRAF) Date: Mon May 14 08:22:01 2007 Subject: [Histonet] job opportunities in Dallas Message-ID: <46481BA7020000DA0000BB54@CNET3.CHILDRENS.COM> Dear Histonetters: We are currently looking for two Histotechnologists to join the team here at Children's Medical Center in Dallas. Here's the info: Children's Medical Center Dallas is currently seeking qualified personnel to fill 2 certified histotech positions. To apply on line and find out more about Children's, visit our website at www.childrens.com Go to Careers, then Job Search; type in Histology Technician and apply on-line. Interviews are initiated through our Human Resources Department only with a completed application. Please let me know if you have questions. Thanks, Linda M Histonet administrator From Susan.Ferrigon <@t> sanofi-aventis.com Mon May 14 09:14:50 2007 From: Susan.Ferrigon <@t> sanofi-aventis.com (Susan.Ferrigon@sanofi-aventis.com) Date: Mon May 14 09:25:39 2007 Subject: [Histonet] Ultrasonic Decalcifying Unit Message-ID: <90B6684A9D6DAF468F7A5DC148754E1DA2E683@ALPW31.f2.enterprise> Hi Is anyone familiar with the Medite/Bios USE 33 Ultrasonic Decalcifying Unit??? if so do you have any suggestions for a starting point for length of time in decal for rat or mouse bones??? Thanks Susan From Karen.Heckford <@t> CHW.edu Mon May 14 10:57:54 2007 From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF) Date: Mon May 14 10:58:09 2007 Subject: [Histonet] CD 56 Message-ID: Does anyone use a CD56 with the Dako Envision Dual Link on FFPE human tissue? If so who are you purchasing it from? Cheers, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathology Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu From adelsyscarol <@t> yahoo.com Mon May 14 11:04:52 2007 From: adelsyscarol <@t> yahoo.com (Carol Wilson) Date: Mon May 14 11:04:56 2007 Subject: [Histonet] Switching from hospital histo to Research Message-ID: <701563.77668.qm@web36906.mail.mud.yahoo.com> Hi All, I'm contemplating making a switch from a routine hospital histology department to supervising a pharmaceutical research histology dept. working with mostly rodent tissue. Any opinions, suggestions, or resources anyone would like to contribute would be greatly appreciated. It seems like a much less stress level than a hospital is at times,..... so what am I missing or not thinking about as far as the "problems" in this type of histology? Thanks in advance, Carol --------------------------------- Sick sense of humor? Visit Yahoo! TV's Comedy with an Edge to see what's on, when. From MAUGER <@t> email.chop.edu Mon May 14 11:33:51 2007 From: MAUGER <@t> email.chop.edu (Joanne Mauger) Date: Mon May 14 11:34:34 2007 Subject: [Histonet] CD 56 Message-ID: HI, we use CD56 fromSanta Cruz (SC-7326) with Dako envision dual link with success-retrieve with citrate pH6. Jo Mauger From rjbuesa <@t> yahoo.com Mon May 14 11:12:39 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon May 14 11:39:23 2007 Subject: [Histonet] Switching from hospital histo to Research In-Reply-To: <701563.77668.qm@web36906.mail.mud.yahoo.com> Message-ID: <568203.91980.qm@web61224.mail.yahoo.com> Condider: how many people to supervise? How much budget to manage? Your new salary and benefits? Any personal experience on rodent tissues? Equipment in the new lab? Ren? J. Carol Wilson wrote: Hi All, I'm contemplating making a switch from a routine hospital histology department to supervising a pharmaceutical research histology dept. working with mostly rodent tissue. Any opinions, suggestions, or resources anyone would like to contribute would be greatly appreciated. It seems like a much less stress level than a hospital is at times,..... so what am I missing or not thinking about as far as the "problems" in this type of histology? Thanks in advance, Carol --------------------------------- Sick sense of humor? Visit Yahoo! TV's Comedy with an Edge to see what's on, when. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss an email again! Yahoo! Toolbar alerts you the instant new Mail arrives. Check it out. From Linda_Coomer <@t> paloscommunityhospital.org Mon May 14 12:07:05 2007 From: Linda_Coomer <@t> paloscommunityhospital.org (Coomer, Linda) Date: Mon May 14 12:07:10 2007 Subject: [Histonet] vapor monitoring Message-ID: <61E44BBE9F5E12429A720E5EB2DF94F9017EFE29@pchms1.pch.org> I am inquiring how the majority of the "histology world" monitors vapors. Currently the hospital has an outside agency twice a year. I would like to monitor by the vapor badges. Are they as effective as the outside agency? Linda Coomer Supervisor of Histology/Cytology Linda_Coomer@Paloscommunityhospital.org Palos Community Hospital 708-923-5094 This message and accompanying documents are covered by the Electronic Communications Privacy Act and the Health Insurance Portability and Accountability Act. This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is confidential and/or privileged. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by e-mail, and delete the original message. Thank you. From lfitzgibbon <@t> huronmedicalcenter.org Mon May 14 12:16:58 2007 From: lfitzgibbon <@t> huronmedicalcenter.org (Linda Fitzgibbon) Date: Mon May 14 12:15:02 2007 Subject: [Histonet] leather strops Message-ID: Hello everyone, I am a 'new' Histonetter and I have a problem. Sturkey, inc suggested that I try you. We still hand strop our microtome knives, after being reconditioned by Sturkey's. I know I'm looking for dinosaurs, but I would like to find the two strops for the Lipshaw Model A hand strop.Thermo/Fisher says they are no longer available. Can anyone help? Thanks, Lynda from Huron Medical Center, Bad Axe, MI From gcallis <@t> montana.edu Mon May 14 12:35:20 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon May 14 12:35:36 2007 Subject: [Histonet] leather strops In-Reply-To: References: Message-ID: <6.0.0.22.1.20070514113105.01b43bf0@gemini.msu.montana.edu> We have never leather stropped a reconditioned knife. When it comes back to us, it is like brand new knife and ready to go. In fact, we have not stropped a knife in over 35 years with the advent of automatic knife sharpeners, in particular the ThermoFisher/ThermoShandon/Shandon sharpener and the superb reconditioning services available to us. At 11:16 AM 5/14/2007, you wrote: >Hello everyone, I am a 'new' Histonetter and I have a problem. Sturkey, inc >suggested that I try you. We still hand strop our microtome knives, after >being reconditioned by Sturkey's. I know I'm looking for dinosaurs, but I >would like to find the two strops for the Lipshaw Model A hand >strop.Thermo/Fisher says they are no longer available. Can anyone help? >Thanks, Lynda from Huron Medical Center, Bad Axe, MI Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From pathderm <@t> yahoo.com Mon May 14 12:36:12 2007 From: pathderm <@t> yahoo.com (bobby king) Date: Mon May 14 12:36:16 2007 Subject: [Histonet] Position Available Message-ID: <760170.31176.qm@web50007.mail.re2.yahoo.com> How do I submit information for an available position within the laboratory. Thanks Bob King, P.A. Laboratory Manager North Georgia Dermatopathology, P.C. --------------------------------- Park yourself in front of a world of choices in alternative vehicles. Visit the Yahoo! Auto Green Center. From christina.thurby <@t> bms.com Mon May 14 13:13:00 2007 From: christina.thurby <@t> bms.com (Christina Thurby) Date: Mon May 14 13:13:06 2007 Subject: [Histonet] Re: Switching from hospital histo to Research (Carol Wilson) In-Reply-To: <0JI100JFXJMN9X@chimera.bms.com> References: <0JI100JFXJMN9X@chimera.bms.com> Message-ID: <4648A6AC.402@bms.com> > 6. Switching from hospital histo to Research (Carol Wilson) > > > And don't forget to think about GLP's (Good Laboratory Practices!!) I made the switch from hospital histo to research about 3 years ago and have been very happy. The job is not less stressful - just plain different. Good luck! Christina > > >Condider: > how many people to supervise? How much budget to manage? Your new salary and benefits? Any personal experience on rodent tissues? Equipment in the new lab? > Ren? J. > >Carol Wilson wrote: > Hi All, >I'm contemplating making a switch from a routine hospital histology department to supervising a pharmaceutical research histology dept. working with mostly rodent tissue. Any opinions, suggestions, or resources anyone would like to contribute would be greatly appreciated. It seems like a much less stress level than a hospital is at times,..... so what am I missing or not thinking about as far as the "problems" in this type of histology? >Thanks in advance, >Carol > > >--------------------------------- >Sick sense of humor? Visit Yahoo! TV's Comedy with an Edge to see what's on, when. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Never miss an email again! >Yahoo! Toolbar alerts you the instant new Mail arrives. Check it out. > >------------------------------ > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >End of Histonet Digest, Vol 42, Issue 20 >**************************************** > > From SLB <@t> Stowers-Institute.org Mon May 14 13:13:56 2007 From: SLB <@t> Stowers-Institute.org (Beckham, Sharon) Date: Mon May 14 13:14:11 2007 Subject: [Histonet] Switching from hospital histo to Research In-Reply-To: <701563.77668.qm@web36906.mail.mud.yahoo.com> Message-ID: Hi Carol, I made a switch from clinical to research 2 years ago after having worked in clinical for about 35 years. There is quite an adjustment. Emotionally, I was very connected to the patients and putting out the best work at the best TAT possible (I did all the IHC for a large hospital system). There was a lot of stress, but also a large amount of job satisfaction. Coming over to the "other side" was very difficult for me. In many ways, the stress level is much less in research. And, for quite a while you really feel like you were not the great tech that you had always considered yourself to be. Research ends up being much more challanging than anything you've ever done before. Even something as simple as cutting blocks can be overwhelming. The methods that work for you one day may not work the next. Also, immunos can be very challanging. Something else you do a lot of is cutting serial sections on paraffin and cryo sometimes on tiny mouse or chick embryos that you can't even see with the middle aged eyes. You learn something new everyday and that can be very rewarding. Another nice thing is no weekends and normal working hours and you most likely won't have the strict dress code that you do in hospital settings. It's a different world, but that is not necessarily a bad thing. I have come to love my job and hoping that learning something new all the time will help me stay young and focused. Hope this helps you out a little. Good luck to you. Sharon -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Wilson Sent: Monday, May 14, 2007 11:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Switching from hospital histo to Research Hi All, I'm contemplating making a switch from a routine hospital histology department to supervising a pharmaceutical research histology dept. working with mostly rodent tissue. Any opinions, suggestions, or resources anyone would like to contribute would be greatly appreciated. It seems like a much less stress level than a hospital is at times,..... so what am I missing or not thinking about as far as the "problems" in this type of histology? Thanks in advance, Carol --------------------------------- Sick sense of humor? Visit Yahoo! TV's Comedy with an Edge to see what's on, when. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From chiggerson <@t> memhosp.com Mon May 14 13:20:07 2007 From: chiggerson <@t> memhosp.com (chiggerson@memhosp.com) Date: Mon May 14 13:20:18 2007 Subject: [Histonet] Histology Position, St. Louis Missouri In-Reply-To: <20070510164727.24794.qmail@mail.memhosp.com> Message-ID: DesPeres Hospital, St. Louis MO has an opening for a Full Time Day Histology Tech. No weekends or call. Excellent salary and benefits. Apply online at www.despereshospital.com under careers tab. From b-frederick <@t> northwestern.edu Mon May 14 13:21:59 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon May 14 13:22:42 2007 Subject: [Histonet] leather strops In-Reply-To: Message-ID: <000001c79654$c3117930$d00f7ca5@lurie.northwestern.edu> Time for disposable blades!!!!! Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda Fitzgibbon Sent: Monday, May 14, 2007 11:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] leather strops Hello everyone, I am a 'new' Histonetter and I have a problem. Sturkey, inc suggested that I try you. We still hand strop our microtome knives, after being reconditioned by Sturkey's. I know I'm looking for dinosaurs, but I would like to find the two strops for the Lipshaw Model A hand strop.Thermo/Fisher says they are no longer available. Can anyone help? Thanks, Lynda from Huron Medical Center, Bad Axe, MI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ploykasek <@t> phenopath.com Mon May 14 13:38:11 2007 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Mon May 14 13:39:05 2007 Subject: [Histonet] Histology Position, St. Louis Missouri In-Reply-To: Message-ID: Wow, must be a Monday. When I first read the web site address, I read it as desperatehospital.com. I thought finally - truth in advertising. Then realized my mis-read. Oh well, a quick laugh. Good luck on filling your position. Patti Loykasek > DesPeres Hospital, St. Louis MO has an opening for a Full Time Day > Histology Tech. No weekends or call. Excellent salary and benefits. > Apply online at www.despereshospital.com under careers tab. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From jlinda <@t> ces.clemson.edu Mon May 14 13:48:01 2007 From: jlinda <@t> ces.clemson.edu (Linda Jenkins) Date: Mon May 14 13:48:11 2007 Subject: [Histonet] RE:Switching from hospital histo to Research Message-ID: <6.2.3.4.2.20070514144036.03b772e8@mailhost.ces.clemson.edu> Hi, Carol! You stated: " I'm contemplating making a switch from a routine hospital histology department to supervising a pharmaceutical research histology dept. working with mostly rodent tissue. Any opinions, suggestions, or resources anyone would like to contribute would be greatly appreciated. It seems like a much less stress level than a hospital is at times,..... so what am I missing or not thinking about as far as the "problems" in this type of histology?" Well, I made the switch 18 years ago and the thoughts of going back to clinical have never entered my mind. What's not to like? Better pay, better benefits, MUCH less stress, no more working holidays or weekends unless I choose to do so. I think the primary ingredient to a successful transfer is that you must be self motivated and capable of independent work. In clinical your days are fairly well defined (e.g. embed, section, stain, etc.). In research, you just never know what each day will bring. If you like orderly, routine days then you might want to stay in clinical. I brought human protocols to my research lab and they all had to be severely modified. Rodent tissue is so lean you must modify processing protocols or you will end up with (as Gayle Callis says) "crispy critters". You will be asked to perform stains you have only read about and they will probably need to be modified on top of that. The only thing I really miss is being able to consult with a pathologist and histology colleagues when I encounter problems. Part of that problem was solved by joining NSH's VIR & Hard Tissue committees where I have bunches of "bonehead buddies" doing the stuff I am. I still haven't found a research pathologist - much to my chagrin. I thought I was happy in clinical histology until I entered the world of research. Now, I know true happiness! Hope this helps, Linda From alaskagirl1950 <@t> yahoo.com Mon May 14 14:07:16 2007 From: alaskagirl1950 <@t> yahoo.com (Patricia Adams) Date: Mon May 14 14:08:36 2007 Subject: [Histonet] RE:Switching from hospital histo to Research In-Reply-To: <6.2.3.4.2.20070514144036.03b772e8@mailhost.ces.clemson.edu> Message-ID: <931152.4274.qm@web52508.mail.re2.yahoo.com> Hello Carol, I am new to the research area, I do clinical histology for the Vet Clinic. But also help the researchers with their Histology needs. It was challenging as far as ordering was concerned, no budget. But have worked that out by charging the researchers and using that money for supplies. The people I work with are very laid back and every minute is not watched. I get my work out and that is all that is asked of me. I enjoy the freedom. It challenges the mind, and you redefine yourself. But a question, as all of us are leaving the hospital, what are they going to do? Carol, I left and glad everyday that I did. Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D ____________________________________________________________________________________Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. http://farechase.yahoo.com/ From jlinda <@t> ces.clemson.edu Mon May 14 14:08:05 2007 From: jlinda <@t> ces.clemson.edu (Linda Jenkins) Date: Mon May 14 14:09:07 2007 Subject: [Histonet] RE:Switching from hospital histo to Research Message-ID: <6.2.3.4.2.20070514135740.03b66d18@mailhost.ces.clemson.edu> Hi, Carol! You stated: " I'm contemplating making a switch from a routine hospital histology department to supervising a pharmaceutical research histology dept. working with mostly rodent tissue. Any opinions, suggestions, or resources anyone would like to contribute would be greatly appreciated. It seems like a much less stress level than a hospital is at times,..... so what am I missing or not thinking about as far as the "problems" in this type of histology?" Well, I made the switch 18 years ago and the thoughts of going back to clinical have never entered my mind. What's not to like? Better pay, better benefits, MUCH less stress, no more working holidays or weekends unless I choose to do so. Being able to attend NSH conventions and regional and state meetings all expenses paid is another perk! I think the primary ingredient to a successful transfer is that you must be self motivated and capable of independent work. In clinical your days are fairly well defined (e.g. embed, section, stain, etc.). In research, you just never know what each day will bring. If you like orderly, routine days then you might want to stay in clinical. I brought human protocols to my research lab and they all had to be severely modified. Rodent tissue is so lean you must modify processing protocols or you will end up with (as Gayle Callis says) "crispy critters". You will be asked to perform stains you have only read about and they will probably need to be modified on top of that. The only thing I really miss is being able to consult with a pathologist and histology colleagues when I encounter problems. Part of that problem was solved by joining NSH's VIR & Hard Tissue committees where I have bunches of "bonehead buddies" doing the stuff I am. I still haven't found a research pathologist - much to my chagrin. There is much to enjoy about each path so, even if you find you don't like research, you can always return to clinical. Good Luck, Linda From psicurello <@t> mcvh-vcu.edu Mon May 14 14:21:45 2007 From: psicurello <@t> mcvh-vcu.edu (Paula Sicurello) Date: Mon May 14 14:22:59 2007 Subject: [Histonet] RE:Switching from hospital histo to Research In-Reply-To: <6.2.3.4.2.20070514135740.03b66d18@mailhost.ces.clemson.edu> References: <6.2.3.4.2.20070514135740.03b66d18@mailhost.ces.clemson.edu> Message-ID: I would love to be back in research. Now I'm doing both cli and research so I have the stress of getting clinical slides out whil e dealing with researchers who wonder why their slides aren't done yet. If anybody needs a research histologist in S. Calif call me! :-) I've set up histo. labs for b Paula :-) -----histonet-bounces@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu From: Linda Jenkins montana.edu Mon May 14 14:30:29 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon May 14 14:31:44 2007 Subject: [Histonet] RE:Switching from hospital histo to Research In-Reply-To: <6.2.3.4.2.20070514144036.03b772e8@mailhost.ces.clemson.edu > References: <6.2.3.4.2.20070514144036.03b772e8@mailhost.ces.clemson.edu> Message-ID: <6.0.0.22.1.20070514131216.01b5ce58@gemini.msu.montana.edu> Dear Linda and Carol, Another thing - develop an invaluable network of research histotechs to help you as you go along. Histonet is one place to find these people, and in the end, you will spend a lot of time with them in very lengthy private conversations. Attending NSH workshops and participating in their committees has been a huge help to our laboratory, right down on how to set up GLP, and just about any other methodology - As for Linda, she is one of my invaluable contacts - sharing ideas, literature, the lost method, problem solving with individuals like her not only boosts your knowledge but you also become good friends along the way and maybe someday co-present workshops on various reserach related topics as we have done. Enjoy your research experience - and remember to avoid those "crispy critters" . Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 At 12:48 PM 5/14/2007, you wrote: >Hi, Carol! > You stated: > > " I'm contemplating making a switch from a routine hospital histology > department to supervising a pharmaceutical research histology dept. > working with mostly rodent tissue. Any opinions, suggestions, or > resources anyone would like to contribute would be greatly > appreciated. It seems like a much less stress level than a hospital is > at times,..... so what am I missing or not thinking about as far as the > "problems" in this type of histology?" > > Well, I made the switch 18 years ago and the thoughts of going > back to clinical have never entered my mind. What's not to like? Better > pay, better benefits, MUCH less stress, no more working holidays or > weekends unless I choose to do so. I think the primary ingredient to a > successful transfer is that you must be self motivated and capable of > independent work. In clinical your days are fairly well defined (e.g. > embed, section, stain, etc.). In research, you just never know what each > day will bring. If you like orderly, routine days then you might want to > stay in clinical. I brought human protocols to my research lab and they > all had to be severely modified. Rodent tissue is so lean you must > modify processing protocols or you will end up with (as Gayle Callis > says) "crispy critters". You will be asked to perform stains you have > only read about and they will probably need to be modified on top of > that. The only thing I really miss is being able to consult with a > pathologist and histology colleagues when I encounter problems. Part of > that problem was solved by joining NSH's VIR & Hard Tissue committees > where I have bunches of "bonehead buddies" doing the stuff I am. I still > haven't found a research pathologist - much to my chagrin. > I thought I was happy in clinical histology until I entered the > world of research. Now, I know true happiness! > > Hope this helps, > Linda > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Mon May 14 14:43:51 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon May 14 14:43:59 2007 Subject: [Histonet] RE:Switching from hospital histo to Research In-Reply-To: <6.2.3.4.2.20070514144036.03b772e8@mailhost.ces.clemson.edu> Message-ID: <000001c79660$3592fa00$d00f7ca5@lurie.northwestern.edu> Carol, I actually work in what my boss calls "A hybrid" We do animal as well as human correlative studies on cancer clinical trials. We are the histo lab for ECOG- basically any clinical trial tissue comes to us for banking and correlative studies on active trials. As for the animal, we do the histo for the many research labs at the university. I have seen a few in my short time here cross over to human. It's neat to read about something out there and realize "hey we had a doctor doing that experiment" How come you don't have a staff pathologist? I have a friend at Abbott and they have one on hand. We have one here for research as well as clinical trials. We also have the luxury that Northwestern Memorial Hospital is across the street!!! Our director has the final approval on antibody titres etc. Bernice Bernice Frederick HTL (ASCP) Sr. Research Tech/Supervisor Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda Jenkins Sent: Monday, May 14, 2007 12:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE:Switching from hospital histo to Research Hi, Carol! You stated: " I'm contemplating making a switch from a routine hospital histology department to supervising a pharmaceutical research histology dept. working with mostly rodent tissue. Any opinions, suggestions, or resources anyone would like to contribute would be greatly appreciated. It seems like a much less stress level than a hospital is at times,..... so what am I missing or not thinking about as far as the "problems" in this type of histology?" Well, I made the switch 18 years ago and the thoughts of going back to clinical have never entered my mind. What's not to like? Better pay, better benefits, MUCH less stress, no more working holidays or weekends unless I choose to do so. I think the primary ingredient to a successful transfer is that you must be self motivated and capable of independent work. In clinical your days are fairly well defined (e.g. embed, section, stain, etc.). In research, you just never know what each day will bring. If you like orderly, routine days then you might want to stay in clinical. I brought human protocols to my research lab and they all had to be severely modified. Rodent tissue is so lean you must modify processing protocols or you will end up with (as Gayle Callis says) "crispy critters". You will be asked to perform stains you have only read about and they will probably need to be modified on top of that. The only thing I really miss is being able to consult with a pathologist and histology colleagues when I encounter problems. Part of that problem was solved by joining NSH's VIR & Hard Tissue committees where I have bunches of "bonehead buddies" doing the stuff I am. I still haven't found a research pathologist - much to my chagrin. I thought I was happy in clinical histology until I entered the world of research. Now, I know true happiness! Hope this helps, Linda _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sandra.Etheridge <@t> gov.bc.ca Mon May 14 15:08:43 2007 From: Sandra.Etheridge <@t> gov.bc.ca (Etheridge, Sandra AL:EX) Date: Mon May 14 15:08:48 2007 Subject: [Histonet] Okajima Stain Message-ID: Hello, everyone, I was wonder if anyone knows if the Okajima stain will differentiate between hemaglobin and myoglobin? Does it even stain myoglobin? Is there a stain or antibody (IHC) for it? Any information is, as always, much appreciated. Sandra Etheridge BC Ministry of Agriculture & Lands Animal Health Center, Histology Abbotsford, BC Canada From elgerp <@t> kadlecmed.org Mon May 14 15:21:40 2007 From: elgerp <@t> kadlecmed.org (Elgert, Phil) Date: Mon May 14 15:21:46 2007 Subject: [Histonet] pathology assistant Message-ID: I am looking for an HT who would like to do path assistant work. This is in south central Washington State. If this sounds interesting call me or Angela Nelson at 946-4611 ext. 2112. Or check us out at kadlecmed.org. Phil Elgert Histology Supervisor Kadlec Medical Center 509-946-7686 elgerp@kadlecmed.org ********************************************************************************************** IMPORTANT: The contents of this email and any attachments are confidential. They are intended for the named recipient(s) only. If you have received this email in error, please notify the system manager or the sender immediately and do not disclose the contents to anyone or make copies thereof. *** eSafe scanned this email for viruses, vandals, and malicious content. *** ********************************************************************************************** From b-frederick <@t> northwestern.edu Mon May 14 15:28:24 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon May 14 15:28:35 2007 Subject: [Histonet] RE:Switching from hospital histo to Research In-Reply-To: <6.0.0.22.1.20070514131216.01b5ce58@gemini.msu.montana.edu> Message-ID: <000501c79666$70014330$d00f7ca5@lurie.northwestern.edu> Addendum to previous: we process most mouse tissue on a 9 hour cycle. Makes quite a difference- very few crispy critters. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Monday, May 14, 2007 1:30 PM To: Linda Jenkins; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE:Switching from hospital histo to Research Dear Linda and Carol, Another thing - develop an invaluable network of research histotechs to help you as you go along. Histonet is one place to find these people, and in the end, you will spend a lot of time with them in very lengthy private conversations. Attending NSH workshops and participating in their committees has been a huge help to our laboratory, right down on how to set up GLP, and just about any other methodology - As for Linda, she is one of my invaluable contacts - sharing ideas, literature, the lost method, problem solving with individuals like her not only boosts your knowledge but you also become good friends along the way and maybe someday co-present workshops on various reserach related topics as we have done. Enjoy your research experience - and remember to avoid those "crispy critters" . Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 At 12:48 PM 5/14/2007, you wrote: >Hi, Carol! > You stated: > > " I'm contemplating making a switch from a routine hospital histology > department to supervising a pharmaceutical research histology dept. > working with mostly rodent tissue. Any opinions, suggestions, or > resources anyone would like to contribute would be greatly > appreciated. It seems like a much less stress level than a hospital is > at times,..... so what am I missing or not thinking about as far as the > "problems" in this type of histology?" > > Well, I made the switch 18 years ago and the thoughts of going > back to clinical have never entered my mind. What's not to like? Better > pay, better benefits, MUCH less stress, no more working holidays or > weekends unless I choose to do so. I think the primary ingredient to a > successful transfer is that you must be self motivated and capable of > independent work. In clinical your days are fairly well defined (e.g. > embed, section, stain, etc.). In research, you just never know what each > day will bring. If you like orderly, routine days then you might want to > stay in clinical. I brought human protocols to my research lab and they > all had to be severely modified. Rodent tissue is so lean you must > modify processing protocols or you will end up with (as Gayle Callis > says) "crispy critters". You will be asked to perform stains you have > only read about and they will probably need to be modified on top of > that. The only thing I really miss is being able to consult with a > pathologist and histology colleagues when I encounter problems. Part of > that problem was solved by joining NSH's VIR & Hard Tissue committees > where I have bunches of "bonehead buddies" doing the stuff I am. I still > haven't found a research pathologist - much to my chagrin. > I thought I was happy in clinical histology until I entered the > world of research. Now, I know true happiness! > > Hope this helps, > Linda > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victor <@t> pathology.washington.edu Mon May 14 15:55:39 2007 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Mon May 14 15:55:43 2007 Subject: [Histonet] New Leica Cassette Labeler Message-ID: <4648CCCB.2030104@pathology.washington.edu> Is there anyone in the Seattle area using the latest labeler with bar codes? Please contact me directly. Victor -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From rjbuesa <@t> yahoo.com Mon May 14 16:19:42 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon May 14 16:19:46 2007 Subject: [Histonet] Okajima Stain In-Reply-To: Message-ID: <150828.40587.qm@web61219.mail.yahoo.com> Sandra: The Okajima stain (1917) was developed to stain [orange] erythrocytes in sections. I don't know about its differential staining for hemoglobin or myoglobin, but I asume it will stain hemoglobin (which was the original intent). Ren? J. "Etheridge, Sandra AL:EX" wrote: Hello, everyone, I was wonder if anyone knows if the Okajima stain will differentiate between hemaglobin and myoglobin? Does it even stain myoglobin? Is there a stain or antibody (IHC) for it? Any information is, as always, much appreciated. Sandra Etheridge BC Ministry of Agriculture & Lands Animal Health Center, Histology Abbotsford, BC Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your own web address. Have a HUGE year through Yahoo! Small Business. From rjbuesa <@t> yahoo.com Mon May 14 16:24:52 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon May 14 16:24:56 2007 Subject: [Histonet] vapor monitoring In-Reply-To: <61E44BBE9F5E12429A720E5EB2DF94F9017EFE29@pchms1.pch.org> Message-ID: <495762.574.qm@web61224.mail.yahoo.com> We also used the services of an outside contractor BUT only after 6 years with an intensive and extensive "in house" monitoring system with badges developed "in house" and others sent outside for develoiping. If you don't know your conditions, just to be in the "safe side", I would try to do a monitoring with badges developed by a company (like KEM). Ren? J. "Coomer, Linda" wrote: I am inquiring how the majority of the "histology world" monitors vapors. Currently the hospital has an outside agency twice a year. I would like to monitor by the vapor badges. Are they as effective as the outside agency? Linda Coomer Supervisor of Histology/Cytology Linda_Coomer@Paloscommunityhospital.org Palos Community Hospital 708-923-5094 This message and accompanying documents are covered by the Electronic Communications Privacy Act and the Health Insurance Portability and Accountability Act. This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is confidential and/or privileged. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by e-mail, and delete the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- You snooze, you lose. Get messages ASAP with AutoCheck in the all-new Yahoo! Mail Beta. From Kitty.Maxey <@t> propathlab.com Mon May 14 16:34:16 2007 From: Kitty.Maxey <@t> propathlab.com (Kitty Maxey) Date: Mon May 14 16:34:20 2007 Subject: [Histonet] Opportunities in Dallas, TX Message-ID: ProPath, a physician-owned pathology practice has openings for experienced Histotechnologists working the night shift. A competitive salary, shift differential and benefits are offered. To apply, please go to our web at www.propathlab.com Kitty Maxey Human Resources Director ProPath - The Leader in Pathology Services 8267 Elmbrook Dr., Suite 100 Dallas, TX 75247 From caramel-bonbon <@t> caramail.com Mon May 14 18:23:24 2007 From: caramel-bonbon <@t> caramail.com (Hired Blade) Date: Mon May 14 18:23:30 2007 Subject: [Histonet] International Employment Scene (Outside USA?) Message-ID: <191988539119106@lycos-europe.com> Hello, I have been reading these posts-c onversations for abo and so far I have not heard very much discussion about the employment scene outside the US. Does anybody have any experi in this, or at least looked into it? I wonder how certification or licensing vary from country to country.& about NGO or Aid organization work? Any leads or i be appreciated. Thanks. [l=] M?me le plus noble des super-h?ros poss?de sa part d'ombre... Lib?rez-la! From jnocito <@t> satx.rr.com Sat May 19 19:07:55 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Mon May 14 19:07:51 2007 Subject: [Histonet] vapor monitoring References: <61E44BBE9F5E12429A720E5EB2DF94F9017EFE29@pchms1.pch.org> Message-ID: <003801c79a72$eb34be00$d49eae18@yourxhtr8hvc4p> Yes. I wasn't sure until I placed a badge over a bucket of formalin for 6 hours. I few days later, I received a telephone call saying they were concerned because my level was over 2.0 ppm. I'm still with that company and I'm still monitoring for formaldehyde and xylene every six months even though CAP says you don't have to once you establish that you are under OSHA standards. (Another reason why I have issues with CAP). Just my 4 cents (inflation you know). I would mention the company in open forum, but y'all know what happens to me when I do that. JTT ----- Original Message ----- From: "Coomer, Linda" To: Sent: Monday, May 14, 2007 12:07 PM Subject: [Histonet] vapor monitoring I am inquiring how the majority of the "histology world" monitors vapors. Currently the hospital has an outside agency twice a year. I would like to monitor by the vapor badges. Are they as effective as the outside agency? Linda Coomer Supervisor of Histology/Cytology Linda_Coomer@Paloscommunityhospital.org Palos Community Hospital 708-923-5094 This message and accompanying documents are covered by the Electronic Communications Privacy Act and the Health Insurance Portability and Accountability Act. This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is confidential and/or privileged. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by e-mail, and delete the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Sat May 19 19:10:51 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Mon May 14 19:10:48 2007 Subject: [Histonet] Histology Position, St. Louis Missouri References: Message-ID: <008b01c79a73$53e56e90$d49eae18@yourxhtr8hvc4p> hmmmmmmmm, and what did we do this wekend? Fess up girl. JTT ----- Original Message ----- From: "Patti Loykasek" To: "histonet" Sent: Monday, May 14, 2007 1:38 PM Subject: Re: [Histonet] Histology Position, St. Louis Missouri > Wow, must be a Monday. When I first read the web site address, I read it > as > desperatehospital.com. I thought finally - truth in advertising. Then > realized my mis-read. Oh well, a quick laugh. Good luck on filling your > position. > > Patti Loykasek > > >> DesPeres Hospital, St. Louis MO has an opening for a Full Time Day >> Histology Tech. No weekends or call. Excellent salary and benefits. >> Apply online at www.despereshospital.com under careers tab. >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------------------------------------------------- > This e-mail message, including any attachments, is for the sole use of > the intended recipients and may contain privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. If > you are not the intended recipient, please contact the sender by e-mail > and destroy all copies of the original message, or you may call PhenoPath > Laboratories, Seattle, WA U.S.A. at (206) 374-9000. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Tue May 15 07:39:50 2007 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Tue May 15 07:40:00 2007 Subject: [Histonet] CD 56 In-Reply-To: Message-ID: Karen, I've used my CD56 from Vector/Novacastra (Cat.#VP-C360) with the dual Link with great success. pH 6.0 HIER with Dako Citrate @ a working dilution of 1:100. Good Luck, Glen Dawson BS, HT & QIHC IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Heckford, Karen - SMMC-SF Sent: Monday, May 14, 2007 10:58 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] CD 56 Does anyone use a CD56 with the Dako Envision Dual Link on FFPE human tissue? If so who are you purchasing it from? Cheers, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathology Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu From buckeyedermatology <@t> yahoo.com Tue May 15 07:51:02 2007 From: buckeyedermatology <@t> yahoo.com (J. Cruz) Date: Tue May 15 07:51:11 2007 Subject: [Histonet] ohio histology position Message-ID: <357379.87183.qm@web33214.mail.mud.yahoo.com> full time ,m - f ,8-5 ,permanent.for buckeye dermatology columbus ohio lab,great benefits,please send resume to buckeyedermatology@yahoo.com or call andrew at 614-798-1358 --------------------------------- Get the free Yahoo! toolbar and rest assured with the added security of spyware protection. From adelsyscarol <@t> yahoo.com Tue May 15 09:48:28 2007 From: adelsyscarol <@t> yahoo.com (Carol Wilson) Date: Tue May 15 09:48:34 2007 Subject: [Histonet] Thanks to all, RE: switching to a research lab Message-ID: <140542.85502.qm@web36915.mail.mud.yahoo.com> Once again thanks to all who have responded to my original post about switching to research. I had many great responses both privately and to the list. When I make the plunge (which is looking like the way I'm going) I'm sure I'll be back in touch a lot counting on all of your great advice and wisdom. In the meantime, several of you mentioned GLP, is there a place I can start for more information in this realm? Thanks again, Carol --------------------------------- Need Mail bonding? Go to the Yahoo! Mail Q&A for great tips from Yahoo! Answers users. From caron_fournier <@t> yahoo.ca Tue May 15 09:56:17 2007 From: caron_fournier <@t> yahoo.ca (caron fournier) Date: Tue May 15 09:56:20 2007 Subject: [Histonet] Re: Switching from hospital histo to Research Message-ID: <384742.65289.qm@web35410.mail.mud.yahoo.com> The one comment that I found interesting is that research is "better pay".....not if you work in Canada. Research is less pay (that's why I work both clinical and research)but still less stress (sometimes) as you work according to the needs of the project. All other things that have been mentioned do apply as well like new stains and stains that don't work on animal tissue. Finding protocols that work for your project that has not been done before. And, above all safety and good lab practises. I love the challenges research offers and the variety (no day is ever the same as the next) but I also like the pay of clinical and need to pay the bills. So, as I said in Canada research is less money especially if you work at a university. Caron Fournier, BSc, R.T. Department of Orthopaedics, Division of Orthopaedic Engineering Research, U.B.C. ----- Original Message ---- From: "histonet-request@lists.utsouthwestern.edu" To: histonet@lists.utsouthwestern.edu Sent: Monday, May 14, 2007 5:15:26 PM Subject: Histonet Digest, Vol 42, Issue 21 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. vapor monitoring (Coomer, Linda) 2. leather strops (Linda Fitzgibbon) 3. Re: leather strops (Gayle Callis) 4. Position Available (bobby king) 5. Re: Switching from hospital histo to Research (Carol Wilson) (Christina Thurby) 6. RE: Switching from hospital histo to Research (Beckham, Sharon) 7. Histology Position, St. Louis Missouri (chiggerson@memhosp.com) 8. RE: leather strops (Bernice Frederick) 9. Re: Histology Position, St. Louis Missouri (Patti Loykasek) 10. RE:Switching from hospital histo to Research (Linda Jenkins) 11. Re: RE:Switching from hospital histo to Research (Patricia Adams) 12. RE:Switching from hospital histo to Research (Linda Jenkins) 13. Re: RE:Switching from hospital histo to Research (Paula Sicurello) 14. Re: RE:Switching from hospital histo to Research (Gayle Callis) 15. RE: RE:Switching from hospital histo to Research (Bernice Frederick) 16. Okajima Stain (Etheridge, Sandra AL:EX) 17. pathology assistant (Elgert, Phil) 18. RE: RE:Switching from hospital histo to Research (Bernice Frederick) 19. New Leica Cassette Labeler (Victor Tobias) 20. Re: Okajima Stain (Rene J Buesa) 21. Re: vapor monitoring (Rene J Buesa) 22. Opportunities in Dallas, TX (Kitty Maxey) 23. International Employment Scene (Outside USA?) (Hired Blade) 24. Re: vapor monitoring (Joe Nocito) 25. Re: Histology Position, St. Louis Missouri (Joe Nocito) Be smarter than spam. See how smart SpamGuard is at giving junk email the boot with the All-new Yahoo! Mail at http://mrd.mail.yahoo.com/try_beta?.intl=ca From bwhitaker <@t> brownpathology.com Tue May 15 10:20:14 2007 From: bwhitaker <@t> brownpathology.com (Bonnie Whitaker) Date: Tue May 15 10:19:11 2007 Subject: [Histonet] Re: Switching from hospital histo to Research In-Reply-To: <384742.65289.qm@web35410.mail.mud.yahoo.com> Message-ID: <002901c79704$8a208980$3601a8c0@brownpathology.net> I've never run across a research position that paid more. If I had, I'd probably be there!! Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of caron fournier Sent: Tuesday, May 15, 2007 9:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Switching from hospital histo to Research The one comment that I found interesting is that research is "better pay".....not if you work in Canada. Research is less pay (that's why I work both clinical and research)but still less stress (sometimes) as you work according to the needs of the project. All other things that have been mentioned do apply as well like new stains and stains that don't work on animal tissue. Finding protocols that work for your project that has not been done before. And, above all safety and good lab practises. I love the challenges research offers and the variety (no day is ever the same as the next) but I also like the pay of clinical and need to pay the bills. So, as I said in Canada research is less money especially if you work at a university. Caron Fournier, BSc, R.T. Department of Orthopaedics, Division of Orthopaedic Engineering Research, U.B.C. ----- Original Message ---- From: "histonet-request@lists.utsouthwestern.edu" To: histonet@lists.utsouthwestern.edu Sent: Monday, May 14, 2007 5:15:26 PM Subject: Histonet Digest, Vol 42, Issue 21 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. vapor monitoring (Coomer, Linda) 2. leather strops (Linda Fitzgibbon) 3. Re: leather strops (Gayle Callis) 4. Position Available (bobby king) 5. Re: Switching from hospital histo to Research (Carol Wilson) (Christina Thurby) 6. RE: Switching from hospital histo to Research (Beckham, Sharon) 7. Histology Position, St. Louis Missouri (chiggerson@memhosp.com) 8. RE: leather strops (Bernice Frederick) 9. Re: Histology Position, St. Louis Missouri (Patti Loykasek) 10. RE:Switching from hospital histo to Research (Linda Jenkins) 11. Re: RE:Switching from hospital histo to Research (Patricia Adams) 12. RE:Switching from hospital histo to Research (Linda Jenkins) 13. Re: RE:Switching from hospital histo to Research (Paula Sicurello) 14. Re: RE:Switching from hospital histo to Research (Gayle Callis) 15. RE: RE:Switching from hospital histo to Research (Bernice Frederick) 16. Okajima Stain (Etheridge, Sandra AL:EX) 17. pathology assistant (Elgert, Phil) 18. RE: RE:Switching from hospital histo to Research (Bernice Frederick) 19. New Leica Cassette Labeler (Victor Tobias) 20. Re: Okajima Stain (Rene J Buesa) 21. Re: vapor monitoring (Rene J Buesa) 22. Opportunities in Dallas, TX (Kitty Maxey) 23. International Employment Scene (Outside USA?) (Hired Blade) 24. Re: vapor monitoring (Joe Nocito) 25. Re: Histology Position, St. Louis Missouri (Joe Nocito) Be smarter than spam. See how smart SpamGuard is at giving junk email the boot with the All-new Yahoo! Mail at http://mrd.mail.yahoo.com/try_beta?.intl=ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From angela.mcnabola <@t> ikonisys.com Tue May 15 10:36:51 2007 From: angela.mcnabola <@t> ikonisys.com (Angela McNabola) Date: Tue May 15 10:36:57 2007 Subject: [Histonet] Re: Switching from hospital histo to Research In-Reply-To: <002901c79704$8a208980$3601a8c0@brownpathology.net> Message-ID: <4ECD18F12E443644B1F3C924A20398241BAA9A@ikoexchange.ikonisys.com> I think that you need to differentiate between research in an academic area or small company where the pay may or may not be so good. Or research at a big pharma or biotech. Speaking from experience having previously worked for a pharmaceutical company in the northeast, and recently laid off, research positions at small companies, universities, or in the clinical area average 25-50K LESS! This includes hospitals.......... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonnie Whitaker Sent: Tuesday, May 15, 2007 11:20 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Switching from hospital histo to Research I've never run across a research position that paid more. If I had, I'd probably be there!! Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of caron fournier Sent: Tuesday, May 15, 2007 9:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Switching from hospital histo to Research The one comment that I found interesting is that research is "better pay".....not if you work in Canada. Research is less pay (that's why I work both clinical and research)but still less stress (sometimes) as you work according to the needs of the project. All other things that have been mentioned do apply as well like new stains and stains that don't work on animal tissue. Finding protocols that work for your project that has not been done before. And, above all safety and good lab practises. I love the challenges research offers and the variety (no day is ever the same as the next) but I also like the pay of clinical and need to pay the bills. So, as I said in Canada research is less money especially if you work at a university. Caron Fournier, BSc, R.T. Department of Orthopaedics, Division of Orthopaedic Engineering Research, U.B.C. ----- Original Message ---- From: "histonet-request@lists.utsouthwestern.edu" To: histonet@lists.utsouthwestern.edu Sent: Monday, May 14, 2007 5:15:26 PM Subject: Histonet Digest, Vol 42, Issue 21 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. vapor monitoring (Coomer, Linda) 2. leather strops (Linda Fitzgibbon) 3. Re: leather strops (Gayle Callis) 4. Position Available (bobby king) 5. Re: Switching from hospital histo to Research (Carol Wilson) (Christina Thurby) 6. RE: Switching from hospital histo to Research (Beckham, Sharon) 7. Histology Position, St. Louis Missouri (chiggerson@memhosp.com) 8. RE: leather strops (Bernice Frederick) 9. Re: Histology Position, St. Louis Missouri (Patti Loykasek) 10. RE:Switching from hospital histo to Research (Linda Jenkins) 11. Re: RE:Switching from hospital histo to Research (Patricia Adams) 12. RE:Switching from hospital histo to Research (Linda Jenkins) 13. Re: RE:Switching from hospital histo to Research (Paula Sicurello) 14. Re: RE:Switching from hospital histo to Research (Gayle Callis) 15. RE: RE:Switching from hospital histo to Research (Bernice Frederick) 16. Okajima Stain (Etheridge, Sandra AL:EX) 17. pathology assistant (Elgert, Phil) 18. RE: RE:Switching from hospital histo to Research (Bernice Frederick) 19. New Leica Cassette Labeler (Victor Tobias) 20. Re: Okajima Stain (Rene J Buesa) 21. Re: vapor monitoring (Rene J Buesa) 22. Opportunities in Dallas, TX (Kitty Maxey) 23. International Employment Scene (Outside USA?) (Hired Blade) 24. Re: vapor monitoring (Joe Nocito) 25. Re: Histology Position, St. Louis Missouri (Joe Nocito) Be smarter than spam. See how smart SpamGuard is at giving junk email the boot with the All-new Yahoo! Mail at http://mrd.mail.yahoo.com/try_beta?.intl=ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> u.arizona.edu Tue May 15 10:59:06 2007 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Tue May 15 11:00:08 2007 Subject: [Histonet] Re: Switching from hospital histo to Research In-Reply-To: <4ECD18F12E443644B1F3C924A20398241BAA9A@ikoexchange.ikonisy s.com> References: <002901c79704$8a208980$3601a8c0@brownpathology.net> Message-ID: <4.3.2.7.2.20070515085554.00c959d8@algranth.inbox.email.arizona.edu> Love my job at a university core facility but I don't do it for the pay. I make a lot less than my colleagues in the clinical lab but the benefits and environment help to make up for what I don't get in my paycheck. Andi At 11:36 AM 5/15/2007 -0400, Angela McNabola wrote: >I think that you need to differentiate between research in an academic >area or small company where the pay may or may not be so good. > >Or research at a big pharma or biotech. Speaking from experience having >previously worked for a pharmaceutical company in the northeast, and >recently laid off, research positions at small companies, universities, >or in the clinical area average 25-50K LESS! This includes >hospitals.......... > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonnie >Whitaker >Sent: Tuesday, May 15, 2007 11:20 AM >To: histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] Re: Switching from hospital histo to Research > > >I've never run across a research position that paid more. If I had, I'd >probably be there!! >Bonnie Whitaker > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of caron >fournier >Sent: Tuesday, May 15, 2007 9:56 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Re: Switching from hospital histo to Research > > >The one comment that I found interesting is that research is "better >pay".....not if you work in Canada. Research is less pay (that's why I >work >both clinical and research)but still less stress (sometimes) as you work >according to the needs of the project. All other things that have been >mentioned do apply as well like new stains and stains that don't work on >animal tissue. Finding protocols that work for your project that has not >been done before. And, above all safety and good lab practises. I love >the >challenges research offers and the variety (no day is ever the same as >the >next) but I also like the pay of clinical and need to pay the bills. >So, as I said in Canada research is less money especially if you work at >a >university. > >Caron Fournier, BSc, R.T. >Department of Orthopaedics, >Division of Orthopaedic Engineering Research, >U.B.C. > > > > >----- Original Message ---- >From: "histonet-request@lists.utsouthwestern.edu" > >To: histonet@lists.utsouthwestern.edu >Sent: Monday, May 14, 2007 5:15:26 PM >Subject: Histonet Digest, Vol 42, Issue 21 > > >Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > >To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > >You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > >When replying, please edit your Subject line so it is more specific than >"Re: Contents of Histonet digest..." > > >Today's Topics: > > 1. vapor monitoring (Coomer, Linda) > 2. leather strops (Linda Fitzgibbon) > 3. Re: leather strops (Gayle Callis) > 4. Position Available (bobby king) > 5. Re: Switching from hospital histo to Research (Carol Wilson) > (Christina Thurby) > 6. RE: Switching from hospital histo to Research (Beckham, Sharon) > 7. Histology Position, St. Louis Missouri (chiggerson@memhosp.com) > 8. RE: leather strops (Bernice Frederick) > 9. Re: Histology Position, St. Louis Missouri (Patti Loykasek) > 10. RE:Switching from hospital histo to Research (Linda Jenkins) > 11. Re: RE:Switching from hospital histo to Research (Patricia Adams) > 12. RE:Switching from hospital histo to Research (Linda Jenkins) > 13. Re: RE:Switching from hospital histo to Research (Paula Sicurello) > 14. Re: RE:Switching from hospital histo to Research (Gayle Callis) > 15. RE: RE:Switching from hospital histo to Research > (Bernice Frederick) > 16. Okajima Stain (Etheridge, Sandra AL:EX) > 17. pathology assistant (Elgert, Phil) > 18. RE: RE:Switching from hospital histo to Research > (Bernice Frederick) > 19. New Leica Cassette Labeler (Victor Tobias) > 20. Re: Okajima Stain (Rene J Buesa) > 21. Re: vapor monitoring (Rene J Buesa) > 22. Opportunities in Dallas, TX (Kitty Maxey) > 23. International Employment Scene (Outside USA?) (Hired Blade) > 24. Re: vapor monitoring (Joe Nocito) > 25. Re: Histology Position, St. Louis Missouri (Joe Nocito) > > > Be smarter than spam. See how smart SpamGuard is at giving junk >email >the boot with the All-new Yahoo! Mail at >http://mrd.mail.yahoo.com/try_beta?.intl=ca >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From Tracy.Bergeron <@t> crl.com Tue May 15 11:11:35 2007 From: Tracy.Bergeron <@t> crl.com (Bergeron, Tracy) Date: Tue May 15 11:11:42 2007 Subject: [Histonet] Re: Switching from hospital histo to Research References: <002901c79704$8a208980$3601a8c0@brownpathology.net> Message-ID: Depends on where geographically you are. In the North East especially Boston area where I am, biotech and pharma pay much much better than university, and more often than not clinical pays more than university research positions do. The salaries at biotech and pharma in this area can be quite high depending on your level of experience. For example.. Someone with more than 10yrs experience in histology, who is certified should be able to find a job in industry that pays somewhere around $60k to $80k a year. But.. Along with the great salary you end up with the marvelous commute into Boston or Cambridge which can take hrs depending on where you are coming from, whether you are driving, taking public transport etc. So it depends on whether the money is worth the commute. University research on the other hand in Boston more often than not pays the same person I mentioned above somewhere around $40k to $50k a year. I work north of Boston in the biotech industry and make a salary that sits in the same range as that of University in Boston. I am considering increasing my commute from 85 miles round trip to 130 miles round trip for the higher salary.. (I live in NH) Tracy E. Bergeron, BS, HT, HTL (ASCP) Histotechnologist Charles River Laboratories Wilmington, MA 978-658-6000 x 1229 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Bonnie Whitaker Sent: Tue 5/15/2007 11:20 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Switching from hospital histo to Research I've never run across a research position that paid more. If I had, I'd probably be there!! Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of caron fournier Sent: Tuesday, May 15, 2007 9:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Switching from hospital histo to Research The one comment that I found interesting is that research is "better pay".....not if you work in Canada. Research is less pay (that's why I work both clinical and research)but still less stress (sometimes) as you work according to the needs of the project. All other things that have been mentioned do apply as well like new stains and stains that don't work on animal tissue. Finding protocols that work for your project that has not been done before. And, above all safety and good lab practises. I love the challenges research offers and the variety (no day is ever the same as the next) but I also like the pay of clinical and need to pay the bills. So, as I said in Canada research is less money especially if you work at a university. Caron Fournier, BSc, R.T. Department of Orthopaedics, Division of Orthopaedic Engineering Research, U.B.C. ----- Original Message ---- From: "histonet-request@lists.utsouthwestern.edu" To: histonet@lists.utsouthwestern.edu Sent: Monday, May 14, 2007 5:15:26 PM Subject: Histonet Digest, Vol 42, Issue 21 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. vapor monitoring (Coomer, Linda) 2. leather strops (Linda Fitzgibbon) 3. Re: leather strops (Gayle Callis) 4. Position Available (bobby king) 5. Re: Switching from hospital histo to Research (Carol Wilson) (Christina Thurby) 6. RE: Switching from hospital histo to Research (Beckham, Sharon) 7. Histology Position, St. Louis Missouri (chiggerson@memhosp.com) 8. RE: leather strops (Bernice Frederick) 9. Re: Histology Position, St. Louis Missouri (Patti Loykasek) 10. RE:Switching from hospital histo to Research (Linda Jenkins) 11. Re: RE:Switching from hospital histo to Research (Patricia Adams) 12. RE:Switching from hospital histo to Research (Linda Jenkins) 13. Re: RE:Switching from hospital histo to Research (Paula Sicurello) 14. Re: RE:Switching from hospital histo to Research (Gayle Callis) 15. RE: RE:Switching from hospital histo to Research (Bernice Frederick) 16. Okajima Stain (Etheridge, Sandra AL:EX) 17. pathology assistant (Elgert, Phil) 18. RE: RE:Switching from hospital histo to Research (Bernice Frederick) 19. New Leica Cassette Labeler (Victor Tobias) 20. Re: Okajima Stain (Rene J Buesa) 21. Re: vapor monitoring (Rene J Buesa) 22. Opportunities in Dallas, TX (Kitty Maxey) 23. International Employment Scene (Outside USA?) (Hired Blade) 24. Re: vapor monitoring (Joe Nocito) 25. Re: Histology Position, St. Louis Missouri (Joe Nocito) Be smarter than spam. See how smart SpamGuard is at giving junk email the boot with the All-new Yahoo! Mail at http://mrd.mail.yahoo.com/try_beta?.intl=ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alaskagirl1950 <@t> yahoo.com Tue May 15 11:18:38 2007 From: alaskagirl1950 <@t> yahoo.com (Patricia Adams) Date: Tue May 15 11:18:46 2007 Subject: [Histonet] Re: Switching from hospital histo to Research In-Reply-To: <384742.65289.qm@web35410.mail.mud.yahoo.com> Message-ID: <461027.59874.qm@web52501.mail.re2.yahoo.com> I work at a University, and do not find that the pay is better than the Hospital. I can not look forward to yearly raises. But less stress has been great. Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D ____________________________________________________________________________________Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: mail, news, photos & more. http://mobile.yahoo.com/go?refer=1GNXIC From mary.gessford <@t> spcorp.com Tue May 15 13:46:02 2007 From: mary.gessford <@t> spcorp.com (Gessford, Mary) Date: Tue May 15 13:46:12 2007 Subject: [Histonet] Thanks to all, RE: switching to a research lab In-Reply-To: <140542.85502.qm@web36915.mail.mud.yahoo.com> Message-ID: Carol, Yes, you can read all about GLPs (21 CRF Part 58 - Good Laboratory Practice for Nonclinical Laboratory Studies) this is the Code of Federal Regulations of the FDA. You can get it online at www.interpharm.com. It is the bible for all of us in the Pharma GLP world. It's a great read, drink lots of coffee. It's a yearly mandatory class for all of us out here in Pharma land. As they(FDA)say....if its not documented it didn't happen! Oh and yes the pay can better but its not like the old days when you start working at 18 and stay at that company for 30 yrs. Their are still a few lucky souls out there, but its rare. I have been in this business for 30yrs this year(time flies when your having fun) and I have been through 7 mergers. 3 in 9 yrs at contract labs that changed hands during the late Seventies/early Eighties and again 4 in 9 yrs with Big Pharma. It is not necessarily a safe place in terms of job security. You may have to move or loss your job, sometimes you don't get the choice. You can guess who last Pfired me. I have actually ridden the WAVE as they say pretty well "then why am I not in La Jolla with Dusko" fate I guess. Its not over yet for many of our Histo friends out there and I feel for all of you, hoping the best as well! I'm in a good place now and have to say I'm learning to love my job. Who said the Northeast pays well.....I have moved here(with the job of course) and you might it pays well(it varies by Company) BUT coming from the mid-west the cost of living is triple. I'm starting to settle in and enjoy the people I work with.....its not for the money right? Although, they don't know what pop is here... they call it soda. People that know me know I don't talk MUCH but I thought I would reply to your GLP question. Good luck! Hi to all my histo friends, you can reach me at my new digs, Mary Gessford Scientist II Supervisor Anatomic Pathology Schering-Plough Corp Summit, NJ 07901 Mary.Gessford@spcorp.com 908-473-4358 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Wilson Sent: Tuesday, May 15, 2007 10:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thanks to all, RE: switching to a research lab Once again thanks to all who have responded to my original post about switching to research. I had many great responses both privately and to the list. When I make the plunge (which is looking like the way I'm going) I'm sure I'll be back in touch a lot counting on all of your great advice and wisdom. In the meantime, several of you mentioned GLP, is there a place I can start for more information in this realm? Thanks again, Carol --------------------------------- Need Mail bonding? Go to the Yahoo! Mail Q&A for great tips from Yahoo! Answers users. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From carmen_loiselle <@t> hotmail.com Tue May 15 13:46:46 2007 From: carmen_loiselle <@t> hotmail.com (carmen loiselle) Date: Tue May 15 13:46:54 2007 Subject: [Histonet] CEACAM 1 Message-ID: Does anyone's using the antibody CEACAM1 (CD66a) from GENOVAC. It's to use on paraffin section on Ventana immunostainers BMK and XT. The researcher brought a very minute amount of this antibody to try on melanoma cases. Any feedback would be greatly appreciated. _________________________________________________________________ Windows Live Hotmail : s?curit? et stockage accrus, et fonctions am?lior?es. Voyez par vous-m?me. www.nouveauhotmail.ca?icid=WLHMFRCA122 From JGREWE <@t> OhioHealth.com Tue May 15 14:05:34 2007 From: JGREWE <@t> OhioHealth.com (JGREWE@OhioHealth.com) Date: Tue May 15 14:05:43 2007 Subject: [Histonet] Jacquelyn Grewe/Staff/OhioHealth is out of the office . Message-ID: I will be out of the office starting 05/15/2007 and will not return until 06/04/2007. I will respond to your message when I return. Thanks, Jackie From hej01 <@t> health.state.ny.us Tue May 15 14:15:27 2007 From: hej01 <@t> health.state.ny.us (Helen E Johnson) Date: Tue May 15 14:15:36 2007 Subject: [Histonet] Prosurfactant Protein C [proSP-C] Message-ID: Hi Histonetters, Does anyone have an IHC protocol for polyclonal proSP-C for FFPE mouse tissue? Helen Johnson (hej01@health.state.ny.us) From LChen <@t> mednet.ucla.edu Tue May 15 14:40:03 2007 From: LChen <@t> mednet.ucla.edu (Chen, Leslie) Date: Tue May 15 14:43:08 2007 Subject: [Histonet] Re: Switching from hospital histo to Research Message-ID: <9F8AE7E7B303F44DB0CAB587F1E96C3F0A58D0A3@admedmail3.ad.medctr.ucla.edu> I work in research and the pay is much worse than the hospital histotech position. I could be making 1.5 times more on the hospital side, but I'd also be working much harder, in pain all the time from the cutting, and work odd hours. My position is much more flexible and I have a great boss. Disadvantages - research relies on grants, and the NIH is cutting money - according to people - due to the war in Iraq. I almost got laid off 1.5 years ago, and may be laid off in June due to lack of funding. ---------------------------------------------------------- IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the recipient, please immediately notify us by return email, and delete this message from your computer. ---------------------------------------------------------- From coco6866 <@t> cox.net Tue May 15 14:43:43 2007 From: coco6866 <@t> cox.net (Coco) Date: Tue May 15 14:43:43 2007 Subject: [Histonet] Processing Message-ID: <000301c79729$58561bc0$021919ac@coco1> have a simple question. When the phrase "processing of specimens"is used in someone's work description(duties), how does everyone interpret that? Example: "Performs routine processing of histology specimens to include accessioning, setting up or performing the gross of surgical specimens and assisting the pathologist at gross." I interpret it is as that person has to accession, gross, process, embed, cut and stain. I want to see if others interpret this like I do. I work for the government and have a situation where my military dept. head and my military immediate supervisor (Head of Anatomical Pathology) created a GS 7 position for a lady who was contracted as a phlebotomist. She has no experience, no knowledge, no degree and is being trained (OJT) which is a no no for a GS 7. Minimal degree for this position is an Associates. I feel this is compromising patient care and I have spoken to my immediate supervisor, who justified this woman getting the position. I have a very tough decision to make, b/c I believe this is wrong and I will not be hung out to dry when something major happens. I'm covering all bases. I know one can not OJT in histology as of Jan. 2004. Does the same rule apply to cytology? This lady is being trained to accession in histology, cyto prep and as a back up transcriptionist. Thanks everybody. Heather A. Harper From Jackie.O'Connor <@t> abbott.com Tue May 15 14:56:53 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Tue May 15 14:57:27 2007 Subject: [Histonet] Re: Switching from hospital histo to Research In-Reply-To: <9F8AE7E7B303F44DB0CAB587F1E96C3F0A58D0A3@admedmail3.ad.medctr.ucla.edu> Message-ID: Sorry for your current situation, Leslie - but I don't know how funding from the NIH has anything to do with the Pentagon. While they are both government entities, I THINK their monies come from separate pockets. I could be wrong, and I welcome naysayers, but it seems like everything is being blamed on the Iraq war these days. Don't let "people" blame everything on the war. Kinda early for Friday flaming, I know - I'm just trying to catch up. I think 'research' has a couple of venues, commercial and academia. I've been a 'player' in hospital histo labs, university settings, as well as big pharma. I like 'em all - but I like big pharma the best. They have the most opportunity for spending money on equipment and personnel - but the politics can be a kicker. I did work in one hospital purported to be the richest private hospital in the world, and money was no object there. I had carte blanche to rebuild that lab - kind nice. Bottom line is, if you like getting up and going to work every day, and you make enough money to live a comfortable life - you have the perfect job. The grass is always greener over the septic tank. "Chen, Leslie" Sent by: histonet-bounces@lists.utsouthwestern.edu 05/15/2007 02:40 PM To cc Subject [Histonet] Re: Switching from hospital histo to Research I work in research and the pay is much worse than the hospital histotech position. I could be making 1.5 times more on the hospital side, but I'd also be working much harder, in pain all the time from the cutting, and work odd hours. My position is much more flexible and I have a great boss. Disadvantages - research relies on grants, and the NIH is cutting money - according to people - due to the war in Iraq. I almost got laid off 1.5 years ago, and may be laid off in June due to lack of funding. ---------------------------------------------------------- IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the recipient, please immediately notify us by return email, and delete this message from your computer. ---------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Tue May 15 15:52:17 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Tue May 15 15:52:31 2007 Subject: [Histonet] Processing In-Reply-To: <000301c79729$58561bc0$021919ac@coco1> Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C5D2@IS-E2K3.grhs.net> A person can be trained to accession in histology, cyto prep and as a back up transcriptionist, she just cannot get certification. I am not sure of the way things are in the military world, but OJT can have its ups and downs. I have two techs the are OJT and both do fine. "processing of specimens" means to take the specimen from accessioning thru cover slipping and everything in the middle. Maybe there is more to the story as to why this supervisor wants this person to have this job! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Coco Sent: Tuesday, May 15, 2007 2:44 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Processing have a simple question. When the phrase "processing of specimens"is used in someone's work description(duties), how does everyone interpret that? Example: "Performs routine processing of histology specimens to include accessioning, setting up or performing the gross of surgical specimens and assisting the pathologist at gross." I interpret it is as that person has to accession, gross, process, embed, cut and stain. I want to see if others interpret this like I do. I work for the government and have a situation where my military dept. head and my military immediate supervisor (Head of Anatomical Pathology) created a GS 7 position for a lady who was contracted as a phlebotomist. She has no experience, no knowledge, no degree and is being trained (OJT) which is a no no for a GS 7. Minimal degree for this position is an Associates. I feel this is compromising patient care and I have spoken to my immediate supervisor, who justified this woman getting the position. I have a very tough decision to make, b/c I believe this is wrong and I will not be hung out to dry when something major happens. I'm covering all bases. I know one can not OJT in histology as of Jan. 2004. Does the same rule apply to cytology? This lady is being trained to accession in histology, cyto prep and as a back up transcriptionist. Thanks everybody. Heather A. Harper _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From coco6866 <@t> cox.net Tue May 15 18:13:04 2007 From: coco6866 <@t> cox.net (Coco) Date: Tue May 15 18:13:06 2007 Subject: [Histonet] Processing Message-ID: <000001c79746$97490b40$021919ac@coco1> Well anybody who is a GS, if you know a friend of a friend, they will get you a job. I agree military believe that civilians are enlisted people, and think that you can be cross trained in every department, like a corpsman. Unfortunately, that is why we have work pd's. Civilian vs. military is not a great interface. A lot of problems are not dealt with until they directly affect that person. Anyways, to the person who said, "report it to CAP". What will they do? At this point, I wish it was easy to say that this person could label slides. The accessioning process after 60 days is a nightmare. I've repeated, visually shown, written things down and this lady is still struggling. But for those who are not GS. A GS 7 can not train a GS 7, nor the secretary at GS 5, can not train a GS 7. The work PD does not state OJT. So right now there is a major violation going on. This lady just happened to want a GS job so badly, she might have sold her soul to the devil to get it and now she is paying dearly. So she is on a year probation, and I'm between a rock and a hard place because she is not capable of performing the job. I have already rattled the pathologists cage, and I am getting ignored. I'm just as guilty of standing by and doing nothing when patient care is being compromised and when there is a problem, military come after the civilian and pin the blame on you. That is why the BS is unreal and very very political. I am debating whether to tell the CO. What should I do? That is a trickle down effect and there will be retaliation. Anybody have any ideas on what I should do? Let it go, or go to the top and than commanders will take some serious heat. Regardless oh how this situation is dealt with the nending is not going to be nice. I don't want this woman to lose her job but than again she is incompetent. All opinions appreciated. Heather From helen.ilsley <@t> uct.ac.za Wed May 16 00:51:22 2007 From: helen.ilsley <@t> uct.ac.za (Helen Ilsley) Date: Wed May 16 00:58:06 2007 Subject: [Histonet] IgE supplier Message-ID: Hi I am looking for a supplier for an antibody that will stain IgE in rabbit tissue. So far I have been unsuccessful, if anyone could help I would really appreciate it. Many thanks Helen Ilsley Helen.Ilsley@uct.ac.za Cardiovascular Research Unit Cape Heart Centre Anzio Road, Observatory, UCT 021-406 6398/6590 021-448 5935 (fax) From eearle <@t> abrazohealth.com Wed May 16 06:46:41 2007 From: eearle <@t> abrazohealth.com (Earle, Elizabeth) Date: Wed May 16 06:46:53 2007 Subject: [Histonet] rodent diet - rather urgent - rather off topic Message-ID: <3961D92A950C8F4CAB37AAC02F21FE30AB1254@mail-srv02.vhswest.local> This is mostly for people in research labs. Have any of your rat or mouse populations exhibited symptoms of the toxicity which dogs and cats experienced due to contaminated pet food? If so, which diet were the animals eating? If any pet rodents have had similar issues, we'd like to know about those as well, and also which diet those animals were on. If you'd like to respond off the list, that is understandable. Thanks so much Elizabeth From Jackie.O'Connor <@t> abbott.com Wed May 16 07:44:20 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed May 16 07:44:55 2007 Subject: [Histonet] Processing In-Reply-To: <000001c79746$97490b40$021919ac@coco1> Message-ID: Heather - If you're not active duty, why don't you get out of that job? Seems like you've been struggling a long time there - -maybe that job just isn't the right fit. Best wishes, Jackie "Coco" Sent by: histonet-bounces@lists.utsouthwestern.edu 05/15/2007 06:13 PM To cc Subject [Histonet] Processing Well anybody who is a GS, if you know a friend of a friend, they will get you a job. I agree military believe that civilians are enlisted people, and think that you can be cross trained in every department, like a corpsman. Unfortunately, that is why we have work pd's. Civilian vs. military is not a great interface. A lot of problems are not dealt with until they directly affect that person. Anyways, to the person who said, "report it to CAP". What will they do? At this point, I wish it was easy to say that this person could label slides. The accessioning process after 60 days is a nightmare. I've repeated, visually shown, written things down and this lady is still struggling. But for those who are not GS. A GS 7 can not train a GS 7, nor the secretary at GS 5, can not train a GS 7. The work PD does not state OJT. So right now there is a major violation going on. This lady just happened to want a GS job so badly, she might have sold her soul to the devil to get it and now she is paying dearly. So she is on a year probation, and I'm between a rock and a hard place because she is not capable of performing the job. I have already rattled the pathologists cage, and I am getting ignored. I'm just as guilty of standing by and doing nothing when patient care is being compromised and when there is a problem, military come after the civilian and pin the blame on you. That is why the BS is unreal and very very political. I am debating whether to tell the CO. What should I do? That is a trickle down effect and there will be retaliation. Anybody have any ideas on what I should do? Let it go, or go to the top and than commanders will take some serious heat. Regardless oh how this situation is dealt with the nending is not going to be nice. I don't want this woman to lose her job but than again she is incompetent. All opinions appreciated. Heather _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Anthony.Gatt <@t> jefferson.edu Wed May 16 08:13:44 2007 From: Anthony.Gatt <@t> jefferson.edu (Anthony Gatt) Date: Wed May 16 08:09:02 2007 Subject: [Histonet] Drosophila sectioning Message-ID: <20070516091344.AHE44215@logan.jefferson.edu> I wrote recently asking for advice on sectioning drosophila heads and many of you wrote back very useful advice pertaining to softening the chitin before processing. My question is...is there anything I can do for the 2 dozen or so blocks that I had already processed and are waiting to be sectioned? These have NOT been processed with softeners but are important specimens. Thanks From jqb7 <@t> CDC.GOV Wed May 16 08:11:07 2007 From: jqb7 <@t> CDC.GOV (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed May 16 08:11:27 2007 Subject: [Histonet] Von Kossa Message-ID: <34BB307EFC9A65429BBB49E330675F7201B0B6D9@LTA3VS003.ees.hhs.gov> Hi everyone, I am looking for a very reliable Von Kossa method for calcium. Is there anything out there that is simple and reliable? Thanks, Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov From rjbuesa <@t> yahoo.com Wed May 16 09:18:16 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 16 09:18:20 2007 Subject: [Histonet] Drosophila sectioning In-Reply-To: <20070516091344.AHE44215@logan.jefferson.edu> Message-ID: <402647.93672.qm@web61215.mail.yahoo.com> Start sectioning the block until you get to the are of interest ("face off"). Remove the block and place it "face down" on the softening liquid during 15 minutes. Cool it and try sectioning again. Ren? J. Anthony Gatt wrote: I wrote recently asking for advice on sectioning drosophila heads and many of you wrote back very useful advice pertaining to softening the chitin before processing. My question is...is there anything I can do for the 2 dozen or so blocks that I had already processed and are waiting to be sectioned? These have NOT been processed with softeners but are important specimens. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: mail, news, photos & more. From rjbuesa <@t> yahoo.com Wed May 16 09:19:15 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 16 09:19:18 2007 Subject: [Histonet] Von Kossa In-Reply-To: <34BB307EFC9A65429BBB49E330675F7201B0B6D9@LTA3VS003.ees.hhs.gov> Message-ID: <334968.46478.qm@web61224.mail.yahoo.com> The method in any histology book is really reliable. Ren? J. "Bartlett, Jeanine (CDC/CCID/NCZVED)" wrote: Hi everyone, I am looking for a very reliable Von Kossa method for calcium. Is there anything out there that is simple and reliable? Thanks, Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ready for the edge of your seat? Check out tonight's top picks on Yahoo! TV. From derek.papalegis <@t> tufts.edu Wed May 16 09:37:08 2007 From: derek.papalegis <@t> tufts.edu (Derek Papalegis) Date: Wed May 16 09:37:12 2007 Subject: [Histonet] slide labels Message-ID: <464B1714.4040804@tufts.edu> I was curious as to where people get their slide labels from? I am looking for xylene resistant labels that are custom made to display the laboratory name on them. Due to our small volume, there is no need to print the slide numbers on a laser printer so all I need is to be able to hand write the slide name on them. Thanks for your help, Derek -- Derek Papalegis Histotechnician T-NEMC Animal Histology Core Division of Laboratory Animal Medicine Tufts University 136 Harrison Avenue Boston, MA 02111 phone: 617 636-2971 fax: 617 636-8354 From mpence <@t> grhs.net Wed May 16 09:50:02 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Wed May 16 09:50:12 2007 Subject: [Histonet] slide labels In-Reply-To: <464B1714.4040804@tufts.edu> Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C5D3@IS-E2K3.grhs.net> Try Shamrock Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Derek Papalegis Sent: Wednesday, May 16, 2007 9:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slide labels I was curious as to where people get their slide labels from? I am looking for xylene resistant labels that are custom made to display the laboratory name on them. Due to our small volume, there is no need to print the slide numbers on a laser printer so all I need is to be able to hand write the slide name on them. Thanks for your help, Derek -- Derek Papalegis Histotechnician T-NEMC Animal Histology Core Division of Laboratory Animal Medicine Tufts University 136 Harrison Avenue Boston, MA 02111 phone: 617 636-2971 fax: 617 636-8354 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Helen.Ilsley <@t> uct.ac.za Wed May 16 00:39:42 2007 From: Helen.Ilsley <@t> uct.ac.za (Helen Ilsley) Date: Wed May 16 11:20:49 2007 Subject: [Histonet] IgE supplier Message-ID: Hi I am looking for a supplier for an antibody that will stain IgE in rabbit tissue. So far I have been unsuccessful, if anyone could help I would really appreciate it. Many thanks Helen Ilsley Helen.Ilsley@uct.ac.za Cardiovascular Research Unit Cape Heart Centre Anzio Road, Observatory, UCT 021-406 6398/6590 021-448 5935 (fax) From PMonfils <@t> Lifespan.org Wed May 16 11:44:12 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed May 16 11:44:17 2007 Subject: [Histonet] Recommended processing schedule for large PMMA specimens? Message-ID: <4EBFF65383B74D49995298C4976D1D5E273C84@LSRIEXCH1.lsmaster.lifespan.org> I have some large femur specimens to process for PMMA (approx. 40x40x60 mm). I have done a fair amount of PMMA work but not with anything this large. What would you recommend for length of time in absolute alcohol, clearing agent, and PMMA infiltration for specimens this size? I am performing all these steps under vacuum. Thanks. From jqb7 <@t> CDC.GOV Wed May 16 11:54:01 2007 From: jqb7 <@t> CDC.GOV (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed May 16 11:54:20 2007 Subject: [Histonet] Thanks! Message-ID: <34BB307EFC9A65429BBB49E330675F7201B0B6E6@LTA3VS003.ees.hhs.gov> Thanks to everyone who has answered my Von Kossa question. Again, the Histonet has proven what a great resource it is. Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov From sbledsoe <@t> iupui.edu Wed May 16 12:05:17 2007 From: sbledsoe <@t> iupui.edu (Bledsoe, Sharon B) Date: Wed May 16 12:05:21 2007 Subject: [Histonet] Need help from Imaging Experts Message-ID: <20070516130517.s3bpk8hktw8044sw@webmail.iu.edu> Here is my problem: I can only use Mac iMovie to edit my digital video. I can not do this on a PC. ONLY iMovie. To save a single frame from the video, my choices are either PICT or JPEG format. My Question is: To save an origianl image, which is the best format, PICT or JPEG? I'm not interested in hearing about Tiffs, BMPs or any other format as that is not one of my choices. From kbowden <@t> ucsd.edu Wed May 16 12:18:16 2007 From: kbowden <@t> ucsd.edu (kbowden) Date: Wed May 16 12:18:26 2007 Subject: [Histonet] Need help from Imaging Experts In-Reply-To: <20070516130517.s3bpk8hktw8044sw@webmail.iu.edu> References: <20070516130517.s3bpk8hktw8044sw@webmail.iu.edu> Message-ID: <464B3CD8.6030905@ucsd.edu> We use macs also but we have to send some images to colleges or send them out for publication, so I always save all of my images as jpeg's because when it is needed they can be opened by a pc. Karen Bowden Staff Research Associate II University of CA, San Diego 9500 Gilman Dr. 0630 La Jolla, CA 92093-0630 858-534-4655 Voice 858-534-5304 Fax kbowden@ucsd.edu CONFIDENTIALITY NOTICE: THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED. IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT THE SENDER ANDDELETE THE MATERIAL FROM ANY COMPUTER. Bledsoe, Sharon B wrote: > Here is my problem: I can only use Mac iMovie to edit my digital > video. I can not do this on a PC. ONLY iMovie. To save a single > frame from the video, my choices are either PICT or JPEG format. > > My Question is: To save an origianl image, which is the best format, > PICT or JPEG? > > I'm not interested in hearing about Tiffs, BMPs or any other format as > that is not one of my choices. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From liz <@t> premierlab.com Wed May 16 12:18:36 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed May 16 12:18:41 2007 Subject: [Histonet] Need help from Imaging Experts In-Reply-To: <20070516130517.s3bpk8hktw8044sw@webmail.iu.edu> Message-ID: <000001c797de$3d1552d0$0d00a8c0@domain.Premier> I would save as a jpeg rather than a pic file. That would be my choice out of those two. Are you going to run image analysis after? If that is the case then you want the file format that will save as much information as possible. For image analysis we save images as tiff files. Standard images we take as jpeg files, since tiff files are too large even to e-mail in some cases. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bledsoe, Sharon B Sent: Wednesday, May 16, 2007 11:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Need help from Imaging Experts Here is my problem: I can only use Mac iMovie to edit my digital video. I can not do this on a PC. ONLY iMovie. To save a single frame from the video, my choices are either PICT or JPEG format. My Question is: To save an origianl image, which is the best format, PICT or JPEG? I'm not interested in hearing about Tiffs, BMPs or any other format as that is not one of my choices. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From djemge <@t> aol.com Wed May 16 12:21:47 2007 From: djemge <@t> aol.com (djemge@aol.com) Date: Wed May 16 12:22:05 2007 Subject: [Histonet] RE: Switching from hospital histo to Research Message-ID: <8C965F5D1864830-169C-3E31@mblk-d48.sysops.aol.com> I have been in clinical histotechnology for over 10 years now and made the switch to research 2 years ago at Northwestern University Feinburg School of Medicine. I absolutely agree with Sharon, Linda and Gayle. There are so many great things I get to do that just would not happen in the clinical setting. The mouse tissue presented some challenges. I figured as a 10 year certified tech I should be able to figure this out myself for godness sake. Most things from human clinical tissue translated, some did not. I had to do some networking and ask questions on the Histonet. Some of the things that I am now doing and hadn't done before are: Fruit flies (softening chitin); aspects of types of mouse tissue (brain, testes, and 11.5 - 14 dpc embryos); in situ for RNA (making all my own reagents and buffers rnase free - intro to DEPC water :-); dealing with new untested antibodies (quite a bit different than the tried and true clinical ab); freezing protocols for fixed frozens and fresh mouse tissue (especially brain, testes, embryos, lung); Mouse perfusion. I have been this awsome clinical tech and boy do I still have a lot to learn. It was unsettling at first. I love my research position at Northwestern. In two years I have expanded my knowledge faster than I ever would have in the clinical grind. It really has made me a more valuable tech. Donna ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From JEllin <@t> yumaregional.org Wed May 16 12:49:43 2007 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Wed May 16 12:49:58 2007 Subject: [Histonet] Need help from Imaging Experts In-Reply-To: <000001c797de$3d1552d0$0d00a8c0@domain.Premier> Message-ID: <29BE166A2CF48D459853F8EC57CD37E8ADA5F7@EXCHANGECLUSTER.yumaregional.local> You are going to find that in time jpeg will loose resolution, and this is a problem. For compression the best form is jpeg , but to store the image in its entirety the best format is TIFF. The problem with TIFF is the file is way to large. What needs to be brought up is an image standard for this type of testing. Similar to what radiology has with DI COMM. Currently DI COMM is looking at a Pathology form of standard imaging. In turn would create a PACS for Pathology. Jesus A. Ellin HT/PA ASCP Yuma Regional Medical Center Histology Systems Technologist Pathology Information Systems 928-336-7444 or 928-336-1144 Fax: 928-336-7319 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Wednesday, May 16, 2007 10:19 AM To: 'Bledsoe, Sharon B'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Need help from Imaging Experts I would save as a jpeg rather than a pic file. That would be my choice out of those two. Are you going to run image analysis after? If that is the case then you want the file format that will save as much information as possible. For image analysis we save images as tiff files. Standard images we take as jpeg files, since tiff files are too large even to e-mail in some cases. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bledsoe, Sharon B Sent: Wednesday, May 16, 2007 11:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Need help from Imaging Experts Here is my problem: I can only use Mac iMovie to edit my digital video. I can not do this on a PC. ONLY iMovie. To save a single frame from the video, my choices are either PICT or JPEG format. My Question is: To save an origianl image, which is the best format, PICT or JPEG? I'm not interested in hearing about Tiffs, BMPs or any other format as that is not one of my choices. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank you. From ccross6032 <@t> aol.com Wed May 16 12:54:18 2007 From: ccross6032 <@t> aol.com (Cheryl Cross) Date: Wed May 16 12:54:39 2007 Subject: [Histonet] Position announcement for NOAA's Center for Marine Animal Health at the University of TN, Knoxville Message-ID: <337DB432-DBDB-4F5A-AD25-2F07C794FF40@aol.com> Hi everyone - This is a new position we are excited to advertise. More information about the position and specifics for applying can be found at this link: http://www.fin.ucar.edu/hr/careers/uco.cfm?do=jobDetailExt&job_ID=857 Thank you! Cheryl Cheryl Cross, DVM, Dipl. ACVP Researcher University Corporation for Atmospheric Research College of Veterinary Medicine University of Tennessee Department of Pathology 2407 River Drive, Room A201 Knoxville, TN 37996-4542 (423) 967-2724 fax: 865-974-5616 ccross@ucar.edu From Boneslides <@t> aol.com Wed May 16 12:58:25 2007 From: Boneslides <@t> aol.com (Boneslides@aol.com) Date: Wed May 16 12:58:40 2007 Subject: [Histonet] NSH Patch for Lab Coat?? Message-ID: I was asked to post this for a co-worker. Is there an NSH patch available to sew onto a lab coat? We checked the NSH website and their "marketplace" page is under construction. Thanks for any help you can provide! ~Diane Diane M. Mahovlic, HT, MLT(ASCP) Orthopedic Pathology & Biomaterials Laboratory Department of Anatomic Pathology The Cleveland Clinic Foundation 9500 Euclid Avenue- L30 Cleveland, Ohio 44195 216-444-0166 ************************************** See what's free at http://www.aol.com. From doug <@t> ppspath.com Wed May 16 13:54:53 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Wed May 16 12:59:44 2007 Subject: [Histonet] Processing In-Reply-To: Message-ID: I agree with Jackie on this Heather. Having worked in an exact environment that you work in (A Navy Hospital) I can tell you that the military and the GS have a totally different mindset than the "real world". I did it for 14 years (I won't tell you which side). Just face up to it because you will always have some sort of a military vs. GS conflict. You can try to rattle the military paths cage but to him you will be "the civilian" and if you make too many waves then you will be a "trouble making civilian". It sounds like you do not have a very supportive GS chain at your hospital. Most likely your GS boss is on the clinical side and considers the AP side the "stepchild" of the lab. Maybe he/she lacks the backbone to support you. It is all about getting through another day without any bother for most. As for the GS PD... I bet you and your worker have a little sentence at the end of it that reads "All other duties assigned", or something like it. The GS pay system is below standard and the benefits are not what they used to be. Is it really worth the headache? No offense to the GS workers out there. I appreciate your tolerance. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Wednesday, May 16, 2007 7:44 AM To: Coco Cc: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Processing Heather - If you're not active duty, why don't you get out of that job? Seems like you've been struggling a long time there - -maybe that job just isn't the right fit. Best wishes, Jackie "Coco" Sent by: histonet-bounces@lists.utsouthwestern.edu 05/15/2007 06:13 PM To cc Subject [Histonet] Processing Well anybody who is a GS, if you know a friend of a friend, they will get you a job. I agree military believe that civilians are enlisted people, and think that you can be cross trained in every department, like a corpsman. Unfortunately, that is why we have work pd's. Civilian vs. military is not a great interface. A lot of problems are not dealt with until they directly affect that person. Anyways, to the person who said, "report it to CAP". What will they do? At this point, I wish it was easy to say that this person could label slides. The accessioning process after 60 days is a nightmare. I've repeated, visually shown, written things down and this lady is still struggling. But for those who are not GS. A GS 7 can not train a GS 7, nor the secretary at GS 5, can not train a GS 7. The work PD does not state OJT. So right now there is a major violation going on. This lady just happened to want a GS job so badly, she might have sold her soul to the devil to get it and now she is paying dearly. So she is on a year probation, and I'm between a rock and a hard place because she is not capable of performing the job. I have already rattled the pathologists cage, and I am getting ignored. I'm just as guilty of standing by and doing nothing when patient care is being compromised and when there is a problem, military come after the civilian and pin the blame on you. That is why the BS is unreal and very very political. I am debating whether to tell the CO. What should I do? That is a trickle down effect and there will be retaliation. Anybody have any ideas on what I should do? Let it go, or go to the top and than commanders will take some serious heat. Regardless oh how this situation is dealt with the nending is not going to be nice. I don't want this woman to lose her job but than again she is incompetent. All opinions appreciated. Heather _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mannis <@t> usgs.gov Wed May 16 13:13:15 2007 From: mannis <@t> usgs.gov (Mandy Annis) Date: Wed May 16 13:13:19 2007 Subject: [Histonet] histology lab purchases Message-ID: Our histology lab is in need of new equipment and we are considering purchasing a new manual microtome, an ultramicrotome, a xylene/alcohol recycler and a slide/cassette labeler. Does anyone have any recommendations? We are an environmental research lab with a small histology laboratory which does not encounter high case loads on a regular basis. We would also welcome information on a company that services microtomes in the midwest (Mid-Missouri). Vendors are welcome to respond. Thank you. Mandy ********************************************************************** Mandy L. Annis Columbia Environmental Research Center 4200 New Haven RD Columbia, MO 65201 Phone:(573)-441-2940 Fax:(573)-876-1896 e-mail:mannis@usgs.gov From oshel1pe <@t> cmich.edu Wed May 16 13:14:52 2007 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Wed May 16 13:15:05 2007 Subject: [Histonet] Need help from Imaging Experts In-Reply-To: <20070516130517.s3bpk8hktw8044sw@webmail.iu.edu> References: <20070516130517.s3bpk8hktw8044sw@webmail.iu.edu> Message-ID: Sharon, I suggest PICT. Short answer: always save original files in a format that does not compress the image *at all*. JPEG compresses images, even if (as in some programs) you have an option to use "0%" compression. (JPG2000 may not use a lossy compression, but I'm not sure about that. I don't trust it.) The saved file can then always be re-named ( ! ) and resaved as a JPEG if needed for e.g. emailing. The reverse is not true. Once an image is compressed, the information -- which means: your data -- discarded during compression is lost forever. I ran a test on my Mac with iMovie, and saved a frame as a JPEG and a PICT. Both files read as 1.5MB, and took 1.5MB memory, but the actual file length of the JPEG was 120kB, while the PICT was 1.5MB. JPEG compression was 1:12, there was no compression of the PICT image (strictly, compression was 1:1). I then opened and saved both images as TIFFS using Graphic Converter**, and could see more detail and better color gradations at 400% zoom in the file originally saved as a PICT. This was also true if just opening the saved PICT and JPEG images. Keep in mind, once the frames have been saved as separate images, they can be opened and then saved in any other format. Adobe Photoshop CS2 (and I think earlier versions) in Windows opens PICT files. The question becomes: what is the purpose of the images? If they are for posting on a web site, emailing, or a Powerpoint presentation, then JPEG is good. If they are for publication in print, or as a poster, then JPEG is no good. JPEG is a lossy file-compression standard. I believe PICT does not compress or lose image information. (We at Microscopy Today don't like JPEG images for this reason, and always ask for micrographs as TIFFs. TIFFs are much larger files than JPEGs, but that is because JPEGs are compressed and have lost information.) Note: iMovie saves at 72dpi if just "Saved", or exported for DV. If the movie is new -- i.e., not yet saved -- don't "Save" it, but "Export" it as "Full Quality". This will leave the individual frames uncompressed for later individual saving. This format can always be resaved for DV or CD-ROM if needed, but you will have kept all the information in the images. **Graphic Converter is a shareware program that started life as a graphics file conversion program and has become an imaging program almost as powerful as Photoshop, but *much* easier to use. And costs $30. Anyone who has a Mac and does images should have this. http://www.lemkesoft.com Phil >Here is my problem: I can only use Mac iMovie to edit my digital >video. I can not do this on a PC. ONLY iMovie. To save a single >frame from the video, my choices are either PICT or JPEG format. > >My Question is: To save an origianl image, which is the best format, >PICT or JPEG? > >I'm not interested in hearing about Tiffs, BMPs or any other format >as that is not one of my choices. -- Philip Oshel Technical Editor, Microscopy Today Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 voice: (989) 774-3576 fax: (989) 774-3462 From Jackie.O'Connor <@t> abbott.com Wed May 16 14:42:40 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed May 16 14:43:59 2007 Subject: [Histonet] Can someone tell me what Berliner Blau means? In-Reply-To: <464B3CD8.6030905@ucsd.edu> Message-ID: Anyone know if "Berliner Blau" is an iron stain? Jackie From mpence <@t> grhs.net Wed May 16 14:55:36 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Wed May 16 14:55:46 2007 Subject: [Histonet] Can someone tell me what Berliner Blau means? In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C5D6@IS-E2K3.grhs.net> Berliner Blau {Prussian blue} German - Iron stain -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Wednesday, May 16, 2007 2:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Can someone tell me what Berliner Blau means? Anyone know if "Berliner Blau" is an iron stain? Jackie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed May 16 15:02:28 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 16 15:02:33 2007 Subject: [Histonet] Can someone tell me what Berliner Blau means? In-Reply-To: Message-ID: <541778.9177.qm@web61211.mail.yahoo.com> Berliner Blau = Berlin Blue and, since Berlin was the capital of Prusia before the unification, not with East Germany (XX century), but under Otto von Bismark (XIX century), it is also know as Prusian Blue. Yes, it is an iron stain. Ren? J. Jackie M O'Connor wrote: Anyone know if "Berliner Blau" is an iron stain? Jackie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. From Jackie.O'Connor <@t> abbott.com Wed May 16 15:04:52 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed May 16 15:09:07 2007 Subject: [Histonet] Can someone tell me what Berliner Blau means? In-Reply-To: <541778.9177.qm@web61211.mail.yahoo.com> Message-ID: wow. You know way too much. Thanks, Rene'. I'm trying to read German slides. Rene J Buesa 05/16/2007 03:02 PM To Jackie M O'Connor , histonet@lists.utsouthwestern.edu cc Subject Re: [Histonet] Can someone tell me what Berliner Blau means? Berliner Blau = Berlin Blue and, since Berlin was the capital of Prusia before the unification, not with East Germany (XX century), but under Otto von Bismark (XIX century), it is also know as Prusian Blue. Yes, it is an iron stain. Ren? J. Jackie M O'Connor wrote: Anyone know if "Berliner Blau" is an iron stain? Jackie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. From ander093 <@t> tc.umn.edu Wed May 16 15:45:59 2007 From: ander093 <@t> tc.umn.edu (LuAnn Anderson) Date: Wed May 16 15:46:10 2007 Subject: [Histonet] Legislative good news for NIH Message-ID: Update on Budget Resolution The first part of FASEB's two-part request has been (nearly) met. Today, House and Senate Conferees to the FY2008 Budget Resolution agreed on a proposal to provide an additional $21 billion for non-defense discretionary programs (Note: FASEB's alert asked Members to support $22 billion). The $21 billion is significant and a victory for the programs that are funded out of the discretionary pie, such as NIH, NSF, DOE, VA, USDA and NASA. The House plans to vote on the budget resolution tomorrow morning, and the Senate hopes to vote on it tomorrow afternoon. The FY2008 Budget Resolution is expected to pass in both Houses of Congress. Once the budget is passed, the Chairmen of the Appropriations Committees in the House and Senate will provide allocations to the twelve appropriations subcommittees. Therefore, you still have time to request that your Members of Congress support the $14 billion increase (over FY2007 levels) for Labor-HHS-Education programs. Thus far, more than 11,000 letters have been sent by FASEB society members to Congress. FASEB's ALERT: http://capwiz.com/faseb/issues/alert/?alertid=9722801&type=CO And, even though we expect Appropriations Chairmen Obey and Byrd to provide allocations in fairly short order, it appears that the appropriations subcommittees in the House will not begin marking up its bills until after the Memorial Day recess. Thanks for your efforts on FASEB's Action Alert! From jnocito <@t> satx.rr.com Mon May 21 17:53:28 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed May 16 17:53:26 2007 Subject: [Histonet] Thanks! References: <34BB307EFC9A65429BBB49E330675F7201B0B6E6@LTA3VS003.ees.hhs.gov> Message-ID: <012c01c79bfa$d99ad240$d49eae18@yourxhtr8hvc4p> no problem, That'll be $1,500.00, cash or credit card only, no checks accepted. I prefer electronic transfers JTT ----- Original Message ----- From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" To: Sent: Wednesday, May 16, 2007 11:54 AM Subject: [Histonet] Thanks! Thanks to everyone who has answered my Von Kossa question. Again, the Histonet has proven what a great resource it is. Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Mon May 21 17:56:13 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed May 16 17:56:12 2007 Subject: [Histonet] slide labels References: <661949901A768E4F9CC16D8AF8F2838CA1C5D3@IS-E2K3.grhs.net> Message-ID: <014801c79bfb$3bc41030$d49eae18@yourxhtr8hvc4p> We purchase our from Label Arts in Kemp TX, 1-800-634-9943. Tell them Joe sent you. On second thought, don't mention me, you'll probably get a better price. Joe ----- Original Message ----- From: "Mike Pence" To: "Derek Papalegis" ; Sent: Wednesday, May 16, 2007 9:50 AM Subject: RE: [Histonet] slide labels Try Shamrock Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Derek Papalegis Sent: Wednesday, May 16, 2007 9:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slide labels I was curious as to where people get their slide labels from? I am looking for xylene resistant labels that are custom made to display the laboratory name on them. Due to our small volume, there is no need to print the slide numbers on a laser printer so all I need is to be able to hand write the slide name on them. Thanks for your help, Derek -- Derek Papalegis Histotechnician T-NEMC Animal Histology Core Division of Laboratory Animal Medicine Tufts University 136 Harrison Avenue Boston, MA 02111 phone: 617 636-2971 fax: 617 636-8354 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anthony <@t> histotechexchange.com Wed May 16 22:22:07 2007 From: anthony <@t> histotechexchange.com (anthony@histotechexchange.com) Date: Wed May 16 22:22:37 2007 Subject: [Histonet] RE: Switching from hospital histo to Research In-Reply-To: <8C965F5D1864830-169C-3E31@mblk-d48.sysops.aol.com> References: <8C965F5D1864830-169C-3E31@mblk-d48.sysops.aol.com> Message-ID: <1493.71.51.6.248.1179372150.squirrel@host4.wfdns.com> Dear All: If anyone out there with some leadership experience is interested in working for an international pharmaceutical company, I have two openings to get in above the ground floor. The positions still entail some cutting, embedding and staining, but your work will be mainly to direct the histologists below you and to provide direction to the pathologists and scientists working with you, on the best path through the range of histology procedures available. Create, document and complete these tasks in compliance with all regulatory bodies involved. If you are interested in finding out more about this position, please contact me. Yours truly, Anthony Williams HT (ASCP) Histotech Exchange LLC 19 Whitmore St. Lexington, VA 24450 T 1 877 464 8911 F 1 540 301 0071 anthony@Histotechexchange.com www.histotechexchange.com I have been in clinical histotechnology for over 10 years now and made the > switch to research 2 years ago at Northwestern University Feinburg School > of Medicine. I absolutely agree with Sharon, Linda and Gayle. There are so > many great things I get to do that just would not happen in the clinical > setting. The mouse tissue presented some challenges. I figured as a 10 > year certified tech I should be able to figure this out myself for > godness sake. Most things from human clinical tissue translated, some did > not. I had to do some networking and ask questions on the Histonet. Some > of the things that I am now doing and hadn't done before are: Fruit flies > (softening chitin); aspects of types of mouse tissue (brain, testes, and > 11.5 - 14 dpc embryos); in situ for RNA (making all my own reagents and > buffers rnase free - intro to DEPC water :-); dealing with new untested > antibodies (quite a bit different than the tried and true clinical ab); > freezing protocols for fixed frozens and fresh mouse tissue (especially > brain, testes, embryos, lung); Mouse perfusion. I have been this awsome > clinical tech and boy do I still have a lot to learn. It was unsettling at > first. I love my research position at Northwestern. In two years I have > expanded my knowledge faster than I ever would have in the clinical grind. > It really has made me a more valuable tech. > > Donna > ________________________________________________________________________ > AOL now offers free email to everyone. Find out more about what's free > from AOL at AOL.com. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From bruce.webber <@t> cefe.cnrs.fr Thu May 17 03:46:50 2007 From: bruce.webber <@t> cefe.cnrs.fr (Bruce Webber) Date: Thu May 17 03:47:09 2007 Subject: [Histonet] Need help from Imaging Experts In-Reply-To: <20070516130517.s3bpk8hktw8044sw@webmail.iu.edu> References: <20070516130517.s3bpk8hktw8044sw@webmail.iu.edu> Message-ID: <464C167A.40506@cefe.cnrs.fr> Hi Sharon, I agree with Phil - PICT is a lossless image format (capable of containing lossless raster as well as vector data) and would be your best bet to retain maximum image information. However, PICT is also a format primarily for Macs and is slowly becoming redundant. To avoid incompatibility issues in the future, I would recommend using graphics software to convert them to an EPS file (if you have inserted vector objects onto your frame captures -e.g. an arrow) or TIFF file (if it is just the single video frame unedited) for long-term archiving. You can then use these archived originals to create smaller (lossy) JPG files for email distribution or web posting. I would suggest reading the following page (and the links it contains) for information on various image file formats: http://en.wikipedia.org/wiki/Image_file_formats Cheers, Bruce On 16/05/2007 18:05, Bledsoe, Sharon B wrote: > Here is my problem: I can only use Mac iMovie to edit my digital > video. I can not do this on a PC. ONLY iMovie. To save a single > frame from the video, my choices are either PICT or JPEG format. > > My Question is: To save an origianl image, which is the best format, > PICT or JPEG? > > I'm not interested in hearing about Tiffs, BMPs or any other format as > that is not one of my choices. > From gu.lang <@t> gmx.at Thu May 17 05:01:50 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu May 17 05:01:57 2007 Subject: AW: [Histonet] Can someone tell me what Berliner Blau means? In-Reply-To: Message-ID: <000301c7986a$63f64df0$6412a8c0@dielangs.at> If you want to know it exactly. Berliner Blau is the substance, that results in the following reaction: 4 FeCl3 + 3 K4Fe(CN)6 --> Fe4[Fe8(CN)6]3 + 12 KCl It is the blue pigment, that was also used for painting. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Jackie M O'Connor Gesendet: Mittwoch, 16. Mai 2007 21:43 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Can someone tell me what Berliner Blau means? Anyone know if "Berliner Blau" is an iron stain? Jackie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From karenadams <@t> comcast.net Thu May 17 07:23:44 2007 From: karenadams <@t> comcast.net (karenadams@comcast.net) Date: Thu May 17 07:23:51 2007 Subject: [Histonet] Question about CBG recycler Message-ID: <051720071223.25632.464C4950000A6FB30000642022058844849C030E0B0E020A9D0E05@comcast.net> Greetings all.....my question about the recycler is this....is it worth the price differential to purchase the 5 year replacement policy as opposed to the 1 year policy???? What types of experiences have labs had w/ problems where they have had to utilize this.....any input would be appreciated.....Karen -- Karen Adams Pathology Laboratories West 9303 Park West Blvd Knoxville, TN 37923 (865) 690-2111 FAX (865) 691-1623 From JWEEMS <@t> sjha.org Thu May 17 08:02:59 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Thu May 17 08:03:29 2007 Subject: [Histonet] Question about CBG recycler In-Reply-To: <051720071223.25632.464C4950000A6FB30000642022058844849C030E0B0E020A9D0E05@comcast.net> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FF98B9@sjhaexc02.sjha.org> We've never had any problems with out recycler, so I would vote no to each. But of course, having said that, you might have problems right away... Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of karenadams@comcast.net Sent: Thursday, May 17, 2007 8:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question about CBG recycler Greetings all.....my question about the recycler is this....is it worth the price differential to purchase the 5 year replacement policy as opposed to the 1 year policy???? What types of experiences have labs had w/ problems where they have had to utilize this.....any input would be appreciated.....Karen -- Karen Adams Pathology Laboratories West 9303 Park West Blvd Knoxville, TN 37923 (865) 690-2111 FAX (865) 691-1623 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From MLashus <@t> pathgroup.com Thu May 17 08:24:38 2007 From: MLashus <@t> pathgroup.com (Mighnon Lashus) Date: Thu May 17 08:24:45 2007 Subject: [Histonet] Question about CBG recycler Message-ID: <7DFAF4868AAAC34C986DF7E1AC16D0260131DCB4@pgnexchg1.pathgroup.com> We had the recycler for 3 years and in those 3 years I had to replace the instrument 3 times. Mighnon Lashus -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Thursday, May 17, 2007 9:03 AM To: karenadams@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Question about CBG recycler We've never had any problems with out recycler, so I would vote no to each. But of course, having said that, you might have problems right away... Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of karenadams@comcast.net Sent: Thursday, May 17, 2007 8:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question about CBG recycler Greetings all.....my question about the recycler is this....is it worth the price differential to purchase the 5 year replacement policy as opposed to the 1 year policy???? What types of experiences have labs had w/ problems where they have had to utilize this.....any input would be appreciated.....Karen -- Karen Adams Pathology Laboratories West 9303 Park West Blvd Knoxville, TN 37923 (865) 690-2111 FAX (865) 691-1623 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. --------------------------------------------------------------------- Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc. immediately at 615-562-9255. From Andrew.Prior <@t> Smith-Nephew.com Thu May 17 08:27:10 2007 From: Andrew.Prior <@t> Smith-Nephew.com (Prior, Andrew) Date: Thu May 17 08:27:17 2007 Subject: [Histonet] Milestone microwave processor Message-ID: <7028DD15E14FDC4DB1F3C5AF8735AF3701BD1E81@EHS021.wound.san> Greetings all, Does anyone out there have any experience using the Milestone RHS-1 microwave processor? I'd be interested to know how good it is for decalcifying bone, processing skin and bone samples and performing epitope retrieval. (Feel free to contact me privately if you want to avoid getting flamed like JTT.) Any feedback about other microwave processors and "Microwaves for the art of Microscopy" by Kok and Boon would also be greatly appreciated. Thanks in advance Andrew Andrew Prior Histologist Smith &Nephew Research Centre York Science Park Heslington York YO10 5DF UK Andrew.Prior@smith-nephew.com 01904 824022 Confidentiality. This electronic transmission is strictly confidential to Smith & Nephew and intended solely for the addressee. It may contain information which is covered by legal, professional or other privilege. If you are not the intended addressee, or someone authorised by the intended addressee to receive transmissions on behalf of the addressee, you must not retain, disclose in any form, copy or take any action in reliance on this transmission. If you have received this transmission in error, please notify the sender as soon as possible and destroy this message. Smith & Nephew UK Limited Registered in England and Wales No.4421171 with registered office at 15 Adam Street, London WC2N 6LA From sbreeden <@t> nmda.nmsu.edu Thu May 17 08:48:25 2007 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu May 17 08:48:32 2007 Subject: [Histonet] Friday Fun Fume Message-ID: <4D14F0FC9316DD41972D5F03C070908B8F446D@nmdamailsvr.nmda.ad.nmsu.edu> I'm back from hibernation and have an idea for a new end-of-week grin (hopefully). It's called "Can You Beat This?". Here's mine: I do special stains in an 18x18" "wet bar" sink using an under-counter refrigerator rack as my slide support. Can you beat that? Knock yourself out... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 From cgfields <@t> lexhealth.org Thu May 17 08:48:46 2007 From: cgfields <@t> lexhealth.org (Carole Fields) Date: Thu May 17 08:48:55 2007 Subject: [Histonet] Mopec Grossing center Message-ID: The Mopec grossing Center is still for sale. It is referbished and ready to use. It is the 500 Series ...the one in the picture is a 600. I will include a picture of the unit. You can call 1-843-588-2559 for information. Thank you Mike _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. From shroffsm <@t> vcu.edu Thu May 17 09:24:15 2007 From: shroffsm <@t> vcu.edu (Seema Shroff) Date: Thu May 17 09:24:31 2007 Subject: [Histonet] Lysosomal / late endosomal marker Message-ID: Hey, What is a good marker to label lysosomes / late endosomes in Central Nervous System TISSUE SECTIONS. I'm trying to label this compartment in oligodendrocytes in spinal cord cross sections - frozen or vibratome. Seema. From japoteete <@t> saintfrancis.com Thu May 17 09:44:14 2007 From: japoteete <@t> saintfrancis.com (Poteete, Jacquie A.) Date: Thu May 17 09:44:21 2007 Subject: [Histonet] Friday Fun Fume In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B8F446D@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: We're close to that. Our main IHC work area is an old shelf placed across the top of two open drawers. Jacquie Poteete MT(ASCP)QIHC Tulsa, OK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, May 17, 2007 8:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Friday Fun Fume I'm back from hibernation and have an idea for a new end-of-week grin (hopefully). It's called "Can You Beat This?". Here's mine: I do special stains in an 18x18" "wet bar" sink using an under-counter refrigerator rack as my slide support. Can you beat that? Knock yourself out... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> u.arizona.edu Thu May 17 09:57:14 2007 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Thu May 17 09:58:18 2007 Subject: [Histonet] Friday Fun Fume In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B8F446D@nmdamailsvr.nmda.ad .nmsu.edu> Message-ID: <4.3.2.7.2.20070517075514.00ccf670@algranth.inbox.email.arizona.edu> I recycle used paraffin into cutting boards. They actually work very well. Cafeteria trays work well as the "molds". Andi At 07:48 AM 5/17/2007 -0600, Breeden, Sara wrote: >I'm back from hibernation and have an idea for a new end-of-week grin >(hopefully). It's called "Can You Beat This?". Here's mine: I do >special stains in an 18x18" "wet bar" sink using an under-counter >refrigerator rack as my slide support. Can you beat that? Knock >yourself out... > > > >Sally Breeden, HT(ASCP) > >NM Dept. of Agriculture > >Veterinary Diagnostic Services > >PO Box 700 > >Albuquerque, NM 87106 > >505-841-2576 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From CDunn <@t> mtsinai.on.ca Thu May 17 10:51:23 2007 From: CDunn <@t> mtsinai.on.ca (Dunn, Cyndy) Date: Thu May 17 10:50:28 2007 Subject: [Histonet] Sledge micrtome Message-ID: I am looking for a good used sledge or sliding microtome to purchase. Please forward pictures and description if you have one available. Thanks, Cyndy Cyndy Dunn Charge Technologist Mount Sinai Hospital Pathology 600 University Avenue Toronto, Ontario M5G 1X6 Phone: 416-586-4800 X4489 Fax: 416-586-8589 cdunn@mtsinai.on.ca From jjohnson <@t> crispregional.org Thu May 17 10:56:23 2007 From: jjohnson <@t> crispregional.org (Jennifer Johnson) Date: Thu May 17 10:59:23 2007 Subject: [Histonet] Re: Can you top this? Message-ID: <001501c7989b$eb8e41b0$2082010a@main.crispregional.org> I personally do not have it that bad but my friends from Sumter Regional Hospital which was recently destroyed in a tornado have relocated to Central Baptist Church where they are performing gross in the kitchen sink. Fortunately, the church relocated to a bigger building and is no longer using that one (it is now privately owned). From mhorne <@t> upei.ca Thu May 17 13:45:23 2007 From: mhorne <@t> upei.ca (Margaret Horne) Date: Thu May 17 12:49:52 2007 Subject: [Histonet] Can you top this? Message-ID: <464C6A83.23366.88628D@localhost> I keep my diluted antibodies in shell vials but needed a way to keep them organised, so : I got a Ferrraro Roche container from a friend ( clear plastic , rectangular) and the cardboard divider from a - 80 freezer box. Cut the divider to fit and organised the vials in their serial dilutions. Fits beautifully in the door of the fridge. A second similar container holds my small bottles of streptavidin and my Biotinylated secondary, etc. My friend thinks I should get another box of those chocolates and charge it to my boss. I haven't had the nerve. My incubation chambers for IHC are rubbermaid containers. For TEM IHC I got two ceramic escargo dishes, each has 6 wells, for $2 in a grocery store sale bin. I was tickled as the ones from an EM catalogue are about $15 each. But my favourite is the wooden stir stick with a eyelash attached to the end by a dab of nail polish that I use with my $3000 diamond knife to do TEM work. The incongruity always makes me chuckle. I like this thread. It's more than a good chuckle. It's an exchange of neat ideas. thanks , margaret Margaret Horne , Histology Teaching Assistant, Dept. of B.SC., Atlantic Veterinary College, U.P.E.I., 550 University Ave., Charlottetown, P.E.I., C1A 4P3 Canada From mjlco <@t> aol.com Thu May 17 13:49:51 2007 From: mjlco <@t> aol.com (mjlco@aol.com) Date: Thu May 17 13:50:01 2007 Subject: [Histonet] Please remove In-Reply-To: References: Message-ID: <8C966CB491EAE11-16D8-1665@webmail-db17.sysops.aol.com> I am off on holiday and dont want to fill up my box. Thanks, matt Longmont united hospital ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From CIngles <@t> uwhealth.org Thu May 17 14:42:28 2007 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Thu May 17 14:42:35 2007 Subject: [Histonet] frozen special stains Message-ID: <08A0A863637F1349BBFD83A96B27A50A120042@uwhis-xchng3.uwhis.hosp.wisc.edu> Hello out there! I have a question for the collective histo-brain out there. (resistance is futile). Does anyone do special stains i.e. trichrome and/or elastic stains on frozen tissue (skin)? I have been trying these out using the regular protocols you would use for FFPE tissues. The (FFPE) controls I stain with the frozens come out fine, but the staining of the frozen sections is so intense that it is difficult to interpret. On the trichrome, the epidermis is so dark it looks black. I've tried cutting the incubation times back by as much as half. It helps a bit but is still pretty intense. Are there any special procedures out there to make them turn out more normal looking? For procedure, I cut sections at 5 microns, dry in a 60 oven for 10 min, and fix in 10% formalin for 30 min. Then rinse in running tap for about 5 minutes and perform stain as normal. Help?!? Claire Ingles UW Hospital Madison WI From lyonm <@t> upstate.edu Thu May 17 14:37:09 2007 From: lyonm <@t> upstate.edu (Michael J. Lyon, Ph.D.) Date: Thu May 17 14:54:49 2007 Subject: [Histonet] Help with Tunel Message-ID: <004a01c798ba$c2a571f0$31ae7f8b@lyonoffice> I am looking for some help using the Chemicon ApopTag kit. We have used this successfully in the past on formalin fixed human muscle, frozen sections. We are looking at a different human muscle, processed similarly. However our results are incredibly inconsistent even on the positive control DNAse treated sections of the same muscle. Some of the sections have nothing at all. While other sections have areas of very bright nuclei and other areas are nearly negative. Any help appreciated. Thanks Michael J. Lyon, Ph.D. Otolaryngology Research Lab SUNY Upstate Medical University 750 East Adams Street Syracuse, NY 13210 Voice 315-464-7253 Fax 315-464-5572 From NMP <@t> Stowers-Institute.org Thu May 17 15:30:37 2007 From: NMP <@t> Stowers-Institute.org (Marsh, Nannette) Date: Thu May 17 15:30:49 2007 Subject: [Histonet] Flex Alcohols Message-ID: Hello. We are considering switching to the Flex alcohols produced by Richard Allen. Any comments, especially from research personnel are greatly appreciated. Thanks, Nanne Nanne Marsh Histology Specialist I Stowers Institute for Medical Research From jnocito <@t> satx.rr.com Tue May 22 18:09:49 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu May 17 18:09:53 2007 Subject: [Histonet] Processing References: <5noink$1pe7dlh@clmboh-mx-14.mgw.rr.com> Message-ID: <015a01c79cc6$4ef49d20$d49eae18@yourxhtr8hvc4p> Heather, have you tried talking to Civilian Personnel? I had a GS7 who was a force-hire because another base closed due to BRAC. You talk about a nightmare! Freddy Kruger had nothing on this woman. Civilian Personnel was a saving grace. JTT ----- Original Message ----- From: "Douglas D Deltour" To: "'Jackie M O'Connor'" ; "'Coco'" Cc: ; Sent: Wednesday, May 16, 2007 1:54 PM Subject: RE: [Histonet] Processing >I agree with Jackie on this Heather. Having worked in an exact environment > that you work in (A Navy Hospital) I can tell you that the military and > the > GS have a totally different mindset than the "real world". I did it for 14 > years (I won't tell you which side). Just face up to it because you will > always have some sort of a military vs. GS conflict. You can try to rattle > the military paths cage but to him you will be "the civilian" and if you > make too many waves then you will be a "trouble making civilian". > It sounds like you do not have a very supportive GS chain at your > hospital. > Most likely your GS boss is on the clinical side and considers the AP side > the "stepchild" of the lab. Maybe he/she lacks the backbone to support > you. > It is all about getting through another day without any bother for most. > > As for the GS PD... I bet you and your worker have a little sentence at > the > end of it that reads "All other duties assigned", or something like it. > > The GS pay system is below standard and the benefits are not what they > used > to be. Is it really worth the headache? > > No offense to the GS workers out there. I appreciate your tolerance. > > > Douglas D. Deltour HT(ASCP) > Histology Manager > Professional Pathology Services, PC > One Science Court > Suite 200 > Columbia, SC 29203 > (803)252-1913 > Fax (803)254-3262 > > ***************************************************** > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of the individual or entity to > which it is addressed and may contain information that is privileged, > confidential and exempt from disclosure under applicable law. If the > reader > of this message is not the intended recipient, you are hereby notified > that > any dissemination, distribution, or copying of this communication is > strictly prohibited by law. If you have received this communication in > error, please notify me immediately. > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M > O'Connor > Sent: Wednesday, May 16, 2007 7:44 AM > To: Coco > Cc: Histonet@lists.utsouthwestern.edu; > histonet-bounces@lists.utsouthwestern.edu > Subject: Re: [Histonet] Processing > > Heather - > If you're not active duty, why don't you get out of that job? Seems like > you've been struggling a long time there - -maybe that job just isn't the > right fit. > Best wishes, > Jackie > > > > "Coco" > Sent by: histonet-bounces@lists.utsouthwestern.edu > 05/15/2007 06:13 PM > > To > > cc > > Subject > [Histonet] Processing > > > > > > > Well anybody who is a GS, if you know a friend of a friend, they will > get > you a job. I agree military believe that civilians are enlisted people, > and > think that you can be cross trained in every department, like a corpsman. > Unfortunately, that is why we have work pd's. Civilian vs. military is not > a > great interface. A lot of problems are not dealt with until they directly > affect that person. Anyways, to the person who said, "report it to CAP". > What will they do? At this point, I wish it was easy to say that this > person > could label slides. The accessioning process after 60 days is a nightmare. > I've repeated, visually shown, written things down and this lady is still > struggling. But for those who are not GS. A GS 7 can not train a GS 7, nor > the secretary at GS 5, can not train a GS 7. The work PD does not state > OJT. > So right now there is a major violation going on. This lady just happened > to > want a GS job so badly, she might have sold her soul to the devil to get > it > and now she is paying dearly. So she is on a year probation, and I'm > between > a rock and a hard place because she is not capable of performing the job. > I > have already rattled the pathologists cage, and I am getting ignored. I'm > just as guilty of standing by and doing nothing when patient care is being > compromised and when there is a problem, military come after the civilian > and pin the blame on you. That is why the BS is unreal and very very > political. I am debating whether to tell the CO. What should I do? That is > a > trickle down effect and there will be retaliation. Anybody have any ideas > on > what I should do? Let it go, or go to the top and than commanders will > take > some serious heat. Regardless oh how this situation is dealt with the > nending is not going to be nice. I don't want this woman to lose her job > but > than again she is incompetent. All opinions appreciated. > > > > Heather > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Fri May 18 04:10:07 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Fri May 18 04:10:25 2007 Subject: [Histonet] Friday Fun Fume In-Reply-To: References: <4D14F0FC9316DD41972D5F03C070908B8F446D@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: When I was a lad we used an electric iron as a hot plate and a toaster to dry the slides in, and warm up our meals, and dry our wet clothes on top of the autoclave never did me any harm! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Poteete, Jacquie A. Sent: 17 May 2007 15:44 To: Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Friday Fun Fume We're close to that. Our main IHC work area is an old shelf placed across the top of two open drawers. Jacquie Poteete MT(ASCP)QIHC Tulsa, OK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, May 17, 2007 8:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Friday Fun Fume I'm back from hibernation and have an idea for a new end-of-week grin (hopefully). It's called "Can You Beat This?". Here's mine: I do special stains in an 18x18" "wet bar" sink using an under-counter refrigerator rack as my slide support. Can you beat that? Knock yourself out... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histology <@t> gradymem.org Fri May 18 07:20:01 2007 From: histology <@t> gradymem.org (histology@gradymem.org) Date: Fri May 18 07:20:14 2007 Subject: [Histonet] Friday Fun Fume In-Reply-To: References: <4D14F0FC9316DD41972D5F03C070908B8F446D@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: When they first opened a pathology lab here (30+ yrs ago) the histology lab was in a former bathroom. You could do gross, put it in the processor, embed, cut, and stain just by swiveling the chair around. And you had to go through the pathologist's office to enter and leave the "lab"...and he was quite a bear in those days!! Angie Barnett, HTL(ASCP) Grady Memorial Hospital Pathology Department 405/224-2258 histology@gradymem.org ----- Original Message ----- From: "Edwards, R.E." Date: Friday, May 18, 2007 4:17 am Subject: RE: [Histonet] Friday Fun Fume To: "Poteete, Jacquie A." , "Breeden, Sara" , histonet@lists.utsouthwestern.edu > When I was a lad we used an electric iron as a hot plate > and a toaster to dry the slides in, and warm up our > meals, and > dry our wet clothes on top of the autoclave never did me any > harm! > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Poteete,Jacquie A. > Sent: 17 May 2007 15:44 > To: Breeden, Sara; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Friday Fun Fume > > We're close to that. Our main IHC work area is an old shelf placed > across the top of two open drawers. > > Jacquie Poteete MT(ASCP)QIHC > Tulsa, OK > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Breeden,Sara > Sent: Thursday, May 17, 2007 8:48 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Friday Fun Fume > > > I'm back from hibernation and have an idea for a new end-of-week grin > (hopefully). It's called "Can You Beat This?". Here's mine: I do > special stains in an 18x18" "wet bar" sink using an under-counter > refrigerator rack as my slide support. Can you beat that? Knock > yourself out... > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lpaveli1 <@t> hurleymc.com Fri May 18 08:25:24 2007 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Fri May 18 08:25:47 2007 Subject: [Histonet] Friday Fun Fume Message-ID: <464D7105020000EE00014376@smtp-gw.hurleymc.com> Back in '71, when I was a student, we were in 1 room with 2 windows on the same side of the building so there was no cross venilation. The box fan was propped up in the window in front of the docs when they grossed blowing out, winter or summer. I still remember going home so "high" from the xylene fumes that it took about 30 minutes to come around. Never remembered driving home and if I ever went through a red light or not!!! Some guardian angel, huh!! Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810-257-9948 fax: 810-762-7082 From POWELL_SA <@t> Mercer.edu Fri May 18 09:29:22 2007 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Fri May 18 09:30:31 2007 Subject: [Histonet] Friday Fun Fume In-Reply-To: <464D7105020000EE00014376@smtp-gw.hurleymc.com> Message-ID: <01MGQ7YQSQBK8WWLGQ@Macon2.Mercer.edu> 45 years ago (coming this July 18th) when I began training (I was 2 at the time :) ), it was in the basement of the hospital in the animal room where they used to keep the rabbits for the pregnancy tests, no windows, not much ventilation if any, and we dried our slides with a hair dryer (yes they had those back then) and we stored all the specimens in jars of formalin in the closet next to that room, so now you know why I am defective, or is it just fixed. Xylene fumes, I can't smell anymore and my liver enzymes run high, not me. Oh well that could be from the wine consumption. My salary at that time would not buy groceries today for a week and gas is out of the question. Call me an antique, not old. Shirley Powell -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Friday, May 18, 2007 9:25 AM To: histology@gradymem.org; ree3@leicester.ac.uk Cc: histonet@lists.utsouthwestern.edu; sbreeden@nmda.nmsu.edu Subject: Re: RE: [Histonet] Friday Fun Fume Back in '71, when I was a student, we were in 1 room with 2 windows on the same side of the building so there was no cross venilation. The box fan was propped up in the window in front of the docs when they grossed blowing out, winter or summer. I still remember going home so "high" from the xylene fumes that it took about 30 minutes to come around. Never remembered driving home and if I ever went through a red light or not!!! Some guardian angel, huh!! Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810-257-9948 fax: 810-762-7082 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mickie25 <@t> netzero.net Fri May 18 10:28:43 2007 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Fri May 18 10:28:56 2007 Subject: [SPAM] Re: RE: [Histonet] Friday Fun Fume In-Reply-To: References: <4D14F0FC9316DD41972D5F03C070908B8F446D@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: My first 'job' was a volunteer in my last semester in high school in 1965. I volunteered to take on a 'work-study' job in the anatomic pathology dept. at UCLA. The job was to change the tops on 6000 (that's right 6K) pint bottles of saved autopsy tissue dating from the 1950's if guess. (Study was whatever I could learn by observation while working). The tissues were saved in Mason jars with metal tops and, you guessed it, the tops had corroded and most of the formalin had evaporated. You can imagine that took me all of that semester and part of the next summer to complete. We had no ventilation in the storage room at all so exposure was constant. Not to mention that occasionally a jar would fall off the back of the shelf and break on the floor. It was a nasty job, but endeared me to the histology staff so they hired me part time as a lab aid to dump tissues, move paraffin blocks and slides, make stains, etc, etc. I worked there through college. We also used chloroform for clearing on the Technicon Duo processors and filled gallon bottles by pumping out of a 55 gallon drum. Got high a few times (no ventilation in the storage room either of course). We didn't wear gloves except when doing autopsies and no hoods for coverslipping. They call these the good ol' days, but I have always questioned that rationale. Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histology@gradymem.org Sent: Friday, May 18, 2007 5:20 AM To: Edwards, R.E. Cc: histonet@lists.utsouthwestern.edu; Breeden, Sara Subject: [SPAM] Re: RE: [Histonet] Friday Fun Fume When they first opened a pathology lab here (30+ yrs ago) the histology lab was in a former bathroom. You could do gross, put it in the processor, embed, cut, and stain just by swiveling the chair around. And you had to go through the pathologist's office to enter and leave the "lab"...and he was quite a bear in those days!! Angie Barnett, HTL(ASCP) Grady Memorial Hospital Pathology Department 405/224-2258 histology@gradymem.org ----- Original Message ----- From: "Edwards, R.E." Date: Friday, May 18, 2007 4:17 am Subject: RE: [Histonet] Friday Fun Fume To: "Poteete, Jacquie A." , "Breeden, Sara" , histonet@lists.utsouthwestern.edu > When I was a lad we used an electric iron as a hot plate > and a toaster to dry the slides in, and warm up our > meals, and > dry our wet clothes on top of the autoclave never did me any > harm! > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Poteete,Jacquie A. > Sent: 17 May 2007 15:44 > To: Breeden, Sara; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Friday Fun Fume > > We're close to that. Our main IHC work area is an old shelf placed > across the top of two open drawers. > > Jacquie Poteete MT(ASCP)QIHC > Tulsa, OK > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Breeden,Sara > Sent: Thursday, May 17, 2007 8:48 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Friday Fun Fume > > > I'm back from hibernation and have an idea for a new end-of-week grin > (hopefully). It's called "Can You Beat This?". Here's mine: I do > special stains in an 18x18" "wet bar" sink using an under-counter > refrigerator rack as my slide support. Can you beat that? Knock > yourself out... > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Fri May 18 10:36:06 2007 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri May 18 10:36:12 2007 Subject: [Histonet] Can You Beat This? Message-ID: <4D14F0FC9316DD41972D5F03C070908B8F4477@nmdamailsvr.nmda.ad.nmsu.edu> I've gotten some interesting, funny, and scary stories about Ye Olde Days. As one of my old bosses used to say, "If I'd known I was going to live this long, I'da taken better care of myself." Too bad all these stories about how we used to do things put us at risk! This should be a lesson to all the newbies out there to wear gloves, masks and question stuff, right? We shouldn't be afraid of what we do, just very cautious of what might come back to bite us. For example, one of my first jobs while training was changing the Technicon Duo - the dioxane vats. Aargh! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 From Jackie.O'Connor <@t> abbott.com Fri May 18 10:38:59 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri May 18 10:47:00 2007 Subject: [Histonet] CSI Las Vegas In-Reply-To: Message-ID: Anyone catch the histology scene in CSI last night? The medical examiner took a piece of skin from a victim, put it in a mold, poured wax on it, put it in a microtome, turned the wheel backwards to get a section on a slide, melted the wax off with a Bunsen burner, then looked at the section under the microscope - -from that he determined that the victim was electrocuted. I love TV science. Happy Friday! Jackie O' From doug <@t> ppspath.com Fri May 18 11:58:12 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Fri May 18 10:57:17 2007 Subject: [Histonet] CSI Las Vegas In-Reply-To: Message-ID: I believe that it was fresh tissue also (a big hunk). He is amazing! Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Friday, May 18, 2007 10:39 AM To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] CSI Las Vegas Anyone catch the histology scene in CSI last night? The medical examiner took a piece of skin from a victim, put it in a mold, poured wax on it, put it in a microtome, turned the wheel backwards to get a section on a slide, melted the wax off with a Bunsen burner, then looked at the section under the microscope - -from that he determined that the victim was electrocuted. I love TV science. Happy Friday! Jackie O' _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Fri May 18 10:56:50 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri May 18 10:57:20 2007 Subject: [Histonet] Can You Beat This? In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B8F4477@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FF9908@sjhaexc02.sjha.org> We had a gallon bucket of xylene mixed with acetone to clean the cassettes in. I spilled it once - in a corner - got drunk as a skunk cleaning it up. I was always zoned out on xylene in those days and didn't even know to enjoy it! I had to sharpen knives on the AO sharpener with the wheel and the powder. My first child did flips when I stood there with her pushed up against that thing while I was pregnant. It's a wonder she can hear today! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Breeden, Sara Sent: Friday, May 18, 2007 11:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Can You Beat This? I've gotten some interesting, funny, and scary stories about Ye Olde Days. As one of my old bosses used to say, "If I'd known I was going to live this long, I'da taken better care of myself." Too bad all these stories about how we used to do things put us at risk! This should be a lesson to all the newbies out there to wear gloves, masks and question stuff, right? We shouldn't be afraid of what we do, just very cautious of what might come back to bite us. For example, one of my first jobs while training was changing the Technicon Duo - the dioxane vats. Aargh! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From akemiat3377 <@t> yahoo.com Fri May 18 10:59:38 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Fri May 18 10:59:41 2007 Subject: [Histonet] CSI Las Vegas In-Reply-To: Message-ID: <504527.154.qm@web31303.mail.mud.yahoo.com> I didn't catch the show last night, but years ago, there was a show called Quincy...very similar to CSI. My best friend Ed Brock was the technical consultant for that show. He worked for Technicon at that time and made sure they were atleast close to being on track. Times change. Akemi Allison-Tacha --- Jackie M O'Connor wrote: > Anyone catch the histology scene in CSI last night? > The medical examiner > took a piece of skin from a victim, put it in a > mold, poured wax on it, > put it in a microtome, turned the wheel backwards to > get a section on a > slide, melted the wax off with a Bunsen burner, then > looked at the section > under the microscope - -from that he determined that > the victim was > electrocuted. I love TV science. > > Happy Friday! > > Jackie O' > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From POWELL_SA <@t> Mercer.edu Fri May 18 10:58:54 2007 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Fri May 18 11:00:14 2007 Subject: [Histonet] CSI Las Vegas In-Reply-To: Message-ID: <01MGQB3QSGHQ8WWKRX@Macon2.Mercer.edu> Yes, you would think that with all that elaborate expensive equipment they have for diagnostics, toxins, etc, that they would at least have had a processor, slide dryer, stainer and oh my goodness, maybe a histotech, duhhhhh. Some one needs to contact their consultant, I think they are a pathologist. sp -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Friday, May 18, 2007 11:39 AM To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] CSI Las Vegas Anyone catch the histology scene in CSI last night? The medical examiner took a piece of skin from a victim, put it in a mold, poured wax on it, put it in a microtome, turned the wheel backwards to get a section on a slide, melted the wax off with a Bunsen burner, then looked at the section under the microscope - -from that he determined that the victim was electrocuted. I love TV science. Happy Friday! Jackie O' _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tp2 <@t> medicine.wisc.edu Fri May 18 11:42:20 2007 From: tp2 <@t> medicine.wisc.edu (Thomas Pier) Date: Fri May 18 11:43:00 2007 Subject: [Histonet] CSI Las Vegas Message-ID: <464D911C020000DF00007597@gwmail.medicine.wisc.edu> Yeah, my wife had to tell me to quite down when I started shouting about how he was doing it all wrong. The tissue was never fixed or processed. It probably would've been totally fried from the "deparaffinization method" that was used. He turned the wheel on the microtome the wrong way. The slide was also magically stained. The biggest problem though, is that a pathologist actually did his own histology. When was the last time that anybody saw that happen? Happy Friday, Tom >>> "Jackie M O'Connor" 05/18/07 10:38 AM >>> Anyone catch the histology scene in CSI last night? The medical examiner took a piece of skin from a victim, put it in a mold, poured wax on it, put it in a microtome, turned the wheel backwards to get a section on a slide, melted the wax off with a Bunsen burner, then looked at the section under the microscope - -from that he determined that the victim was electrocuted. I love TV science. Happy Friday! Jackie O' _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vazquezr <@t> ohsu.edu Fri May 18 12:42:03 2007 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri May 18 12:42:33 2007 Subject: [Histonet] CSI Las Vegas Message-ID: Tom, Like never (pathologist doing own histo)... ditto happy Friday Robyn >>> "Thomas Pier" 5/18/2007 9:42 AM >>> Yeah, my wife had to tell me to quite down when I started shouting about how he was doing it all wrong. The tissue was never fixed or processed. It probably would've been totally fried from the "deparaffinization method" that was used. He turned the wheel on the microtome the wrong way. The slide was also magically stained. The biggest problem though, is that a pathologist actually did his own histology. When was the last time that anybody saw that happen? Happy Friday, Tom >>> "Jackie M O'Connor" 05/18/07 10:38 AM >>> Anyone catch the histology scene in CSI last night? The medical examiner took a piece of skin from a victim, put it in a mold, poured wax on it, put it in a microtome, turned the wheel backwards to get a section on a slide, melted the wax off with a Bunsen burner, then looked at the section under the microscope - -from that he determined that the victim was electrocuted. I love TV science. Happy Friday! Jackie O' _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> CDC.GOV Fri May 18 12:54:29 2007 From: jqb7 <@t> CDC.GOV (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Fri May 18 12:58:10 2007 Subject: [Histonet] CSI Las Vegas References: Message-ID: <34BB307EFC9A65429BBB49E330675F7201B0AA80@LTA3VS003.ees.hhs.gov> Don't forget the doctors from House taking a piece of tissue from the refrigerator, placing it on a petri dish, dropping red stuff on it and looking under the scope to see the "immuno".....and all of the doctors do all of the procedures themselves, including all the lab work. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Robyn Vazquez Sent: Fri 5/18/2007 1:42 PM To: Jackie.O'Connor@abbott.com; histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu; tp2@medicine.wisc.edu Subject: Re: [Histonet] CSI Las Vegas Tom, Like never (pathologist doing own histo)... ditto happy Friday Robyn >>> "Thomas Pier" 5/18/2007 9:42 AM >>> Yeah, my wife had to tell me to quite down when I started shouting about how he was doing it all wrong. The tissue was never fixed or processed. It probably would've been totally fried from the "deparaffinization method" that was used. He turned the wheel on the microtome the wrong way. The slide was also magically stained. The biggest problem though, is that a pathologist actually did his own histology. When was the last time that anybody saw that happen? Happy Friday, Tom >>> "Jackie M O'Connor" 05/18/07 10:38 AM >>> Anyone catch the histology scene in CSI last night? The medical examiner took a piece of skin from a victim, put it in a mold, poured wax on it, put it in a microtome, turned the wheel backwards to get a section on a slide, melted the wax off with a Bunsen burner, then looked at the section under the microscope - -from that he determined that the victim was electrocuted. I love TV science. Happy Friday! Jackie O' _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Fri May 18 13:00:16 2007 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Fri May 18 13:03:24 2007 Subject: [Histonet] Friday Fun Fume References: <01MGQ7YQSQBK8WWLGQ@Macon2.Mercer.edu> Message-ID: <08A0A863637F1349BBFD83A96B27A50A120044@uwhis-xchng3.uwhis.hosp.wisc.edu> Well I always thought old histotechs never died, they were just well fixed. Oh yea, The histo lab at our main hospital is still in the basement and you have to go UP one floor to get to the morgue! Talk about an ego dropper. By the way, what are windows? :) Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Shirley Powell Sent: Fri 5/18/2007 9:29 AM To: 'Lynette Pavelich'; histology@gradymem.org; ree3@leicester.ac.uk Cc: histonet@lists.utsouthwestern.edu; sbreeden@nmda.nmsu.edu Subject: RE: RE: [Histonet] Friday Fun Fume 45 years ago (coming this July 18th) when I began training (I was 2 at the time :) ), it was in the basement of the hospital in the animal room where they used to keep the rabbits for the pregnancy tests, no windows, not much ventilation if any, and we dried our slides with a hair dryer (yes they had those back then) and we stored all the specimens in jars of formalin in the closet next to that room, so now you know why I am defective, or is it just fixed. Xylene fumes, I can't smell anymore and my liver enzymes run high, not me. Oh well that could be from the wine consumption. My salary at that time would not buy groceries today for a week and gas is out of the question. Call me an antique, not old. Shirley Powell From CIngles <@t> uwhealth.org Fri May 18 13:07:03 2007 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Fri May 18 13:09:18 2007 Subject: [Histonet] CSI Las Vegas References: <464D911C020000DF00007597@gwmail.medicine.wisc.edu> Message-ID: <08A0A863637F1349BBFD83A96B27A50A120045@uwhis-xchng3.uwhis.hosp.wisc.edu> Yea, it's hard enough getting the residents to cut their own frozens! Then we usually have to clean up after them. Is that the part in the job discription that states "...and other duties as required."? Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Thomas Pier Sent: Fri 5/18/2007 11:42 AM To: Jackie.O'Connor@abbott.com; histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] CSI Las Vegas Yeah, my wife had to tell me to quite down when I started shouting about how he was doing it all wrong. The tissue was never fixed or processed. It probably would've been totally fried from the "deparaffinization method" that was used. He turned the wheel on the microtome the wrong way. The slide was also magically stained. The biggest problem though, is that a pathologist actually did his own histology. When was the last time that anybody saw that happen? Happy Friday, Tom From POWELL_SA <@t> Mercer.edu Fri May 18 13:18:54 2007 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Fri May 18 13:20:16 2007 Subject: [Histonet] Friday Fun Fume In-Reply-To: <08A0A863637F1349BBFD83A96B27A50A120044@uwhis-xchng3.uwhis.hosp.wisc.edu> Message-ID: <01MGQG0AXQPI8WWDSK@Macon2.Mercer.edu> Something that runs your computer. sp -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Friday, May 18, 2007 2:00 PM Cc: histonet@lists.utsouthwestern.edu Subject: RE: RE: [Histonet] Friday Fun Fume Well I always thought old histotechs never died, they were just well fixed. Oh yea, The histo lab at our main hospital is still in the basement and you have to go UP one floor to get to the morgue! Talk about an ego dropper. By the way, what are windows? :) Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Shirley Powell Sent: Fri 5/18/2007 9:29 AM To: 'Lynette Pavelich'; histology@gradymem.org; ree3@leicester.ac.uk Cc: histonet@lists.utsouthwestern.edu; sbreeden@nmda.nmsu.edu Subject: RE: RE: [Histonet] Friday Fun Fume 45 years ago (coming this July 18th) when I began training (I was 2 at the time :) ), it was in the basement of the hospital in the animal room where they used to keep the rabbits for the pregnancy tests, no windows, not much ventilation if any, and we dried our slides with a hair dryer (yes they had those back then) and we stored all the specimens in jars of formalin in the closet next to that room, so now you know why I am defective, or is it just fixed. Xylene fumes, I can't smell anymore and my liver enzymes run high, not me. Oh well that could be from the wine consumption. My salary at that time would not buy groceries today for a week and gas is out of the question. Call me an antique, not old. Shirley Powell _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA <@t> Mercer.edu Fri May 18 13:19:55 2007 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Fri May 18 13:21:11 2007 Subject: [Histonet] CSI Las Vegas In-Reply-To: <34BB307EFC9A65429BBB49E330675F7201B0AA80@LTA3VS003.ees.hhs.gov> Message-ID: <01MGQG1KD0IS8WWIH8@Macon2.Mercer.edu> You think they have been talking to CLIA? sp -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Friday, May 18, 2007 1:54 PM To: Robyn Vazquez; Jackie.O'Connor@abbott.com; histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu; tp2@medicine.wisc.edu Subject: RE: [Histonet] CSI Las Vegas Don't forget the doctors from House taking a piece of tissue from the refrigerator, placing it on a petri dish, dropping red stuff on it and looking under the scope to see the "immuno".....and all of the doctors do all of the procedures themselves, including all the lab work. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Robyn Vazquez Sent: Fri 5/18/2007 1:42 PM To: Jackie.O'Connor@abbott.com; histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu; tp2@medicine.wisc.edu Subject: Re: [Histonet] CSI Las Vegas Tom, Like never (pathologist doing own histo)... ditto happy Friday Robyn >>> "Thomas Pier" 5/18/2007 9:42 AM >>> Yeah, my wife had to tell me to quite down when I started shouting about how he was doing it all wrong. The tissue was never fixed or processed. It probably would've been totally fried from the "deparaffinization method" that was used. He turned the wheel on the microtome the wrong way. The slide was also magically stained. The biggest problem though, is that a pathologist actually did his own histology. When was the last time that anybody saw that happen? Happy Friday, Tom >>> "Jackie M O'Connor" 05/18/07 10:38 AM >>> Anyone catch the histology scene in CSI last night? The medical examiner took a piece of skin from a victim, put it in a mold, poured wax on it, put it in a microtome, turned the wheel backwards to get a section on a slide, melted the wax off with a Bunsen burner, then looked at the section under the microscope - -from that he determined that the victim was electrocuted. I love TV science. Happy Friday! Jackie O' _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From swest <@t> statlab.com Fri May 18 13:22:44 2007 From: swest <@t> statlab.com (swest@statlab.com) Date: Fri May 18 13:22:35 2007 Subject: [Histonet] Remove please Message-ID: <14A6B50B8AA247878EBD9663DD0CAC4D.MAI@mail.stormtechgroup.com> to many e mails fulling up my inbox. thanks, Susan From POWELL_SA <@t> Mercer.edu Fri May 18 13:25:23 2007 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Fri May 18 13:26:44 2007 Subject: [Histonet] CSI Las Vegas In-Reply-To: <504527.154.qm@web31303.mail.mud.yahoo.com> Message-ID: <01MGQG8CI4Q88WWDSK@Macon2.Mercer.edu> Akemi, I remember watching Quincy when he told his side kick (I can't remember his name) that his job was going to be "reduced" to becoming a histology tech "receiving and labeling specimens". I quit watching that show after that condescending remark. Your friend Ed would have really gotten flamed if we had histonet then. :) Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Friday, May 18, 2007 12:00 PM To: Jackie M O'Connor; histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Cc: broed@aol.com Subject: Re: [Histonet] CSI Las Vegas I didn't catch the show last night, but years ago, there was a show called Quincy...very similar to CSI. My best friend Ed Brock was the technical consultant for that show. He worked for Technicon at that time and made sure they were atleast close to being on track. Times change. Akemi Allison-Tacha --- Jackie M O'Connor wrote: > Anyone catch the histology scene in CSI last night? > The medical examiner > took a piece of skin from a victim, put it in a mold, poured wax on > it, put it in a microtome, turned the wheel backwards to get a section > on a slide, melted the wax off with a Bunsen burner, then looked at > the section under the microscope - -from that he determined that the > victim was electrocuted. I love TV science. > > Happy Friday! > > Jackie O' > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> CDC.GOV Fri May 18 13:29:19 2007 From: jqb7 <@t> CDC.GOV (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Fri May 18 13:30:02 2007 Subject: [Histonet] CSI Las Vegas References: <01MGQG8CI4Q88WWDSK@Macon2.Mercer.edu> Message-ID: <34BB307EFC9A65429BBB49E330675F7201B0AA81@LTA3VS003.ees.hhs.gov> Good old Sam! He was supposedly a combo. histotech and pathology assistant. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Shirley Powell Sent: Fri 5/18/2007 2:25 PM To: 'Akemi Allison-Tacha'; 'Jackie M O'Connor'; histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Cc: broed@aol.com Subject: RE: [Histonet] CSI Las Vegas Akemi, I remember watching Quincy when he told his side kick (I can't remember his name) that his job was going to be "reduced" to becoming a histology tech "receiving and labeling specimens". I quit watching that show after that condescending remark. Your friend Ed would have really gotten flamed if we had histonet then. :) Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Friday, May 18, 2007 12:00 PM To: Jackie M O'Connor; histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Cc: broed@aol.com Subject: Re: [Histonet] CSI Las Vegas I didn't catch the show last night, but years ago, there was a show called Quincy...very similar to CSI. My best friend Ed Brock was the technical consultant for that show. He worked for Technicon at that time and made sure they were atleast close to being on track. Times change. Akemi Allison-Tacha --- Jackie M O'Connor wrote: > Anyone catch the histology scene in CSI last night? > The medical examiner > took a piece of skin from a victim, put it in a mold, poured wax on > it, put it in a microtome, turned the wheel backwards to get a section > on a slide, melted the wax off with a Bunsen burner, then looked at > the section under the microscope - -from that he determined that the > victim was electrocuted. I love TV science. > > Happy Friday! > > Jackie O' > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From denise.woodward <@t> uconn.edu Fri May 18 13:50:07 2007 From: denise.woodward <@t> uconn.edu (Woodward, Denise) Date: Fri May 18 13:50:14 2007 Subject: [Histonet] CSI Las Vegas In-Reply-To: <464D911C020000DF00007597@gwmail.medicine.wisc.edu> References: <464D911C020000DF00007597@gwmail.medicine.wisc.edu> Message-ID: I actually caught a Cardiology Fellow trying to embed mouse heart tissue from formalin into paraffin. He wanted me to tell him what to do with the formalin when he was finished. He said he was trying to save the department research money by skipping the processing step. This was in a major Boston institution labeled as within the "top 3" in the country! Takes all kinds......... TGIF and start of my VACATION! Yipee! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Pier Sent: Friday, May 18, 2007 12:42 PM To: Jackie.O'Connor@abbott.com; histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] CSI Las Vegas Yeah, my wife had to tell me to quite down when I started shouting about how he was doing it all wrong. The tissue was never fixed or processed. It probably would've been totally fried from the "deparaffinization method" that was used. He turned the wheel on the microtome the wrong way. The slide was also magically stained. The biggest problem though, is that a pathologist actually did his own histology. When was the last time that anybody saw that happen? Happy Friday, Tom >>> "Jackie M O'Connor" 05/18/07 10:38 AM >>> Anyone catch the histology scene in CSI last night? The medical examiner took a piece of skin from a victim, put it in a mold, poured wax on it, put it in a microtome, turned the wheel backwards to get a section on a slide, melted the wax off with a Bunsen burner, then looked at the section under the microscope - -from that he determined that the victim was electrocuted. I love TV science. Happy Friday! Jackie O' _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From denise.woodward <@t> uconn.edu Fri May 18 13:55:29 2007 From: denise.woodward <@t> uconn.edu (Woodward, Denise) Date: Fri May 18 13:55:34 2007 Subject: [Histonet] unsubscribe Message-ID: From sonyprasad <@t> hotmail.com Fri May 18 14:34:21 2007 From: sonyprasad <@t> hotmail.com (sony prasad) Date: Fri May 18 14:34:31 2007 Subject: [Histonet] alpha SMA staining on FFPE sections. Message-ID: Hi, I have the alpha SMA antibody from DAKO and presently looking to optimise staining on FFPE mouse kidney sections. It would be a great help if I could get a protocol for it. Many thanks, Sony. _________________________________________________________________ Tried the new MSN Messenger? It’s cool! Download now. http://messenger.msn.com/Download/Default.aspx?mkt=en-in From lpwenk <@t> sbcglobal.net Fri May 18 15:04:22 2007 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Fri May 18 15:04:50 2007 Subject: [Histonet] CSI Las Vegas In-Reply-To: <01MGQB3QSGHQ8WWKRX@Macon2.Mercer.edu> Message-ID: <000f01c79987$bb1ec8a0$0202a8c0@HPPav2> And to think ASCP is using William Petersen's photo and quote are on the first page of the new ASCP "Careers in the Medical Laboratory" bookelt, to help recruit people into the medical laboratory field. http://www.ascp.org/careerlinks/ Go to bottom of page to download a copy (almost 2 MB, so beware), or to order copies. HT/HTL are on page 5. Any idea what the stain/solution is in the Erlenmeyer? Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley Powell Sent: Friday, May 18, 2007 11:59 AM To: 'Jackie M O'Connor'; histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] CSI Las Vegas Yes, you would think that with all that elaborate expensive equipment they have for diagnostics, toxins, etc, that they would at least have had a processor, slide dryer, stainer and oh my goodness, maybe a histotech, duhhhhh. Some one needs to contact their consultant, I think they are a pathologist. sp -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Friday, May 18, 2007 11:39 AM To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] CSI Las Vegas Anyone catch the histology scene in CSI last night? The medical examiner took a piece of skin from a victim, put it in a mold, poured wax on it, put it in a microtome, turned the wheel backwards to get a section on a slide, melted the wax off with a Bunsen burner, then looked at the section under the microscope - -from that he determined that the victim was electrocuted. I love TV science. Happy Friday! Jackie O' _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Fri May 18 16:04:40 2007 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri May 18 16:05:00 2007 Subject: [Histonet] CSI Las Vegas In-Reply-To: <01MGQG8CI4Q88WWDSK@Macon2.Mercer.edu> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB7143@EMAIL.archildrens.org> I remember that. Sam had made some error and was going to be punished by going to histology. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley Powell Sent: Friday, May 18, 2007 1:25 PM To: 'Akemi Allison-Tacha'; 'Jackie M O'Connor'; histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Cc: broed@aol.com Subject: RE: [Histonet] CSI Las Vegas Akemi, I remember watching Quincy when he told his side kick (I can't remember his name) that his job was going to be "reduced" to becoming a histology tech "receiving and labeling specimens". I quit watching that show after that condescending remark. Your friend Ed would have really gotten flamed if we had histonet then. :) Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Friday, May 18, 2007 12:00 PM To: Jackie M O'Connor; histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Cc: broed@aol.com Subject: Re: [Histonet] CSI Las Vegas I didn't catch the show last night, but years ago, there was a show called Quincy...very similar to CSI. My best friend Ed Brock was the technical consultant for that show. He worked for Technicon at that time and made sure they were atleast close to being on track. Times change. Akemi Allison-Tacha --- Jackie M O'Connor wrote: > Anyone catch the histology scene in CSI last night? > The medical examiner > took a piece of skin from a victim, put it in a mold, poured wax on > it, put it in a microtome, turned the wheel backwards to get a section > on a slide, melted the wax off with a Bunsen burner, then looked at > the section under the microscope - -from that he determined that the > victim was electrocuted. I love TV science. > > Happy Friday! > > Jackie O' > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. 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Thank you. ============================================================================== From jqb7 <@t> CDC.GOV Fri May 18 16:08:46 2007 From: jqb7 <@t> CDC.GOV (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Fri May 18 16:09:14 2007 Subject: [Histonet] CSI Las Vegas References: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB7143@EMAIL.archildrens.org> Message-ID: <34BB307EFC9A65429BBB49E330675F7201B0AA88@LTA3VS003.ees.hhs.gov> God forbid! :) ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Horn, Hazel V Sent: Fri 5/18/2007 5:04 PM To: Shirley Powell; Akemi Allison-Tacha; Jackie M O'Connor; histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Cc: broed@aol.com Subject: RE: [Histonet] CSI Las Vegas I remember that. Sam had made some error and was going to be punished by going to histology. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley Powell Sent: Friday, May 18, 2007 1:25 PM To: 'Akemi Allison-Tacha'; 'Jackie M O'Connor'; histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Cc: broed@aol.com Subject: RE: [Histonet] CSI Las Vegas Akemi, I remember watching Quincy when he told his side kick (I can't remember his name) that his job was going to be "reduced" to becoming a histology tech "receiving and labeling specimens". I quit watching that show after that condescending remark. Your friend Ed would have really gotten flamed if we had histonet then. :) Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Friday, May 18, 2007 12:00 PM To: Jackie M O'Connor; histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Cc: broed@aol.com Subject: Re: [Histonet] CSI Las Vegas I didn't catch the show last night, but years ago, there was a show called Quincy...very similar to CSI. My best friend Ed Brock was the technical consultant for that show. He worked for Technicon at that time and made sure they were atleast close to being on track. Times change. Akemi Allison-Tacha --- Jackie M O'Connor wrote: > Anyone catch the histology scene in CSI last night? > The medical examiner > took a piece of skin from a victim, put it in a mold, poured wax on > it, put it in a microtome, turned the wheel backwards to get a section > on a slide, melted the wax off with a Bunsen burner, then looked at > the section under the microscope - -from that he determined that the > victim was electrocuted. I love TV science. > > Happy Friday! > > Jackie O' > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From opiecurt <@t> yahoo.com Fri May 18 17:02:41 2007 From: opiecurt <@t> yahoo.com (curt tague) Date: Fri May 18 17:02:50 2007 Subject: [Histonet] histology supervisor with experience needed. Message-ID: <38961.80501.qm@web81602.mail.mud.yahoo.com> i am getting really busy and find that i am sometimes ovewhelmed. my lab is growing fast and with an influx of staff i could use a little help keeping things/people in line... scheduling and the like. we're in so cal, (moving to anaheim in less than 2 mos.) do about 500 block/night and will be adding approx 800 more in the next 6-8 months. what kind of salary does that position dictate? if interested, please include you requirements. curt pathology arts --------------------------------- Need a vacation? Get great deals to amazing places on Yahoo! Travel. From liz <@t> premierlab.com Fri May 18 17:05:16 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri May 18 17:05:21 2007 Subject: [Histonet] CLIA recs and contract labs Message-ID: <000c01c79998$9dc65cd0$0d00a8c0@domain.Premier> I have a question about histology labs that just perform the processing of tissue samples from humans for diagnosis. I spoke to two different individuals at the Colorado Department of Public Health and Environment the Laboratory Services Division and they told me that as long as the slides were not being read at my facility then we are not required to be CLIA Certified. The pathologist that I could be performing the work for thinks that I need to be CLIA certified. So what is the correct answer? It would be nice to get some responses from labs that just provide the histology services to a pathologist, but any advice would be helpful. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 From hhawkins <@t> utmb.edu Fri May 18 17:58:24 2007 From: hhawkins <@t> utmb.edu (Hawkins, Hal K.) Date: Fri May 18 17:58:31 2007 Subject: [Histonet] CSI Las Vegas In-Reply-To: <34BB307EFC9A65429BBB49E330675F7201B0AA80@LTA3VS003.ees.hhs.gov> Message-ID: I saw it, and was mainly impressed that the forensic pathologist cut his own sections! I didn't notice that he turned the crank backwards. We used to see that kind of lining up of nuclei as a cautery artifact. I was able to assume that it was selective editing that let him go from the unfixed sample to the microscope in 45 seconds. Hal Hawkins (deputy medical examiner) UTMB, Galveston, Texas -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Friday, May 18, 2007 12:54 PM To: Robyn Vazquez; Jackie.O'Connor@abbott.com; histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu; tp2@medicine.wisc.edu Subject: RE: [Histonet] CSI Las Vegas Don't forget the doctors from House taking a piece of tissue from the refrigerator, placing it on a petri dish, dropping red stuff on it and looking under the scope to see the "immuno".....and all of the doctors do all of the procedures themselves, including all the lab work. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Robyn Vazquez Sent: Fri 5/18/2007 1:42 PM To: Jackie.O'Connor@abbott.com; histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu; tp2@medicine.wisc.edu Subject: Re: [Histonet] CSI Las Vegas Tom, Like never (pathologist doing own histo)... ditto happy Friday Robyn >>> "Thomas Pier" 5/18/2007 9:42 AM >>> Yeah, my wife had to tell me to quite down when I started shouting about how he was doing it all wrong. The tissue was never fixed or processed. It probably would've been totally fried from the "deparaffinization method" that was used. He turned the wheel on the microtome the wrong way. The slide was also magically stained. The biggest problem though, is that a pathologist actually did his own histology. When was the last time that anybody saw that happen? Happy Friday, Tom >>> "Jackie M O'Connor" 05/18/07 10:38 AM >>> Anyone catch the histology scene in CSI last night? The medical examiner took a piece of skin from a victim, put it in a mold, poured wax on it, put it in a microtome, turned the wheel backwards to get a section on a slide, melted the wax off with a Bunsen burner, then looked at the section under the microscope - -from that he determined that the victim was electrocuted. I love TV science. Happy Friday! Jackie O' _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Wed May 23 18:33:49 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri May 18 18:33:53 2007 Subject: [Histonet] Friday Fun Fume References: <4D14F0FC9316DD41972D5F03C070908B8F446D@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <004b01c79d92$d1353660$d49eae18@yourxhtr8hvc4p> Ok, here you go. I know TV is TV but did anyone catch the CSI episode last night? The "doctor" cut a piece of skin off of a "victim, placed it a cassette, filled it with paraffin, then put it on a Lietz microtome, cut a ribbon, placed it on a glass slide, then used a Bunsen burner to fit it to the slide. HELLO, where is the processing people? Who are the technical advisors for this show? No wonder why people think they can do our jobs with depictions like that. I'M fuming. JTT ----- Original Message ----- From: To: "Edwards, R.E." Cc: ; "Breeden, Sara" Sent: Friday, May 18, 2007 7:20 AM Subject: Re: RE: [Histonet] Friday Fun Fume > When they first opened a pathology lab here (30+ yrs ago) the > histology lab was in a former bathroom. You could do gross, put it in > the processor, embed, cut, and stain just by swiveling the chair > around. And you had to go through the pathologist's office to enter > and leave the "lab"...and he was quite a bear in those days!! > > Angie Barnett, HTL(ASCP) > Grady Memorial Hospital > Pathology Department > 405/224-2258 > histology@gradymem.org > > > ----- Original Message ----- > From: "Edwards, R.E." > Date: Friday, May 18, 2007 4:17 am > Subject: RE: [Histonet] Friday Fun Fume > To: "Poteete, Jacquie A." , "Breeden, > Sara" , histonet@lists.utsouthwestern.edu > >> When I was a lad we used an electric iron as a hot plate >> and a toaster to dry the slides in, and warm up our >> meals, and >> dry our wet clothes on top of the autoclave never did me > any >> harm! >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >> Poteete,Jacquie A. >> Sent: 17 May 2007 15:44 >> To: Breeden, Sara; histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] Friday Fun Fume >> >> We're close to that. Our main IHC work area is an old shelf placed >> across the top of two open drawers. >> >> Jacquie Poteete MT(ASCP)QIHC >> Tulsa, OK >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >> Breeden,Sara >> Sent: Thursday, May 17, 2007 8:48 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Friday Fun Fume >> >> >> I'm back from hibernation and have an idea for a new end-of-week grin >> (hopefully). It's called "Can You Beat This?". Here's mine: I do >> special stains in an 18x18" "wet bar" sink using an under-counter >> refrigerator rack as my slide support. Can you beat that? Knock >> yourself out... >> >> >> >> Sally Breeden, HT(ASCP) >> >> NM Dept. of Agriculture >> >> Veterinary Diagnostic Services >> >> PO Box 700 >> >> Albuquerque, NM 87106 >> >> 505-841-2576 >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jason.Wiese <@t> va.gov Fri May 18 18:45:07 2007 From: Jason.Wiese <@t> va.gov (Wiese, Jason VHAROS) Date: Fri May 18 18:45:15 2007 Subject: [Histonet] Friday Fun Fume In-Reply-To: <004b01c79d92$d1353660$d49eae18@yourxhtr8hvc4p> References: <4D14F0FC9316DD41972D5F03C070908B8F446D@nmdamailsvr.nmda.ad.nmsu.edu> <004b01c79d92$d1353660$d49eae18@yourxhtr8hvc4p> Message-ID: <70EEF3D43B3C164C94037D811B2BE193E129DF@VHAV20MSGA3.v20.med.va.gov> Okay, so this is like the 50th message today on this particular matter. I am a man of much reserve, but my mailbox is inundated with junk. Can we perhaps minimize the BS? Just a thought? JW -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, May 23, 2007 4:34 PM To: histology@gradymem.org; Edwards, R.E. Cc: histonet@lists.utsouthwestern.edu; Breeden, Sara Subject: Re: RE: [Histonet] Friday Fun Fume Ok, here you go. I know TV is TV but did anyone catch the CSI episode last night? The "doctor" cut a piece of skin off of a "victim, placed it a cassette, filled it with paraffin, then put it on a Lietz microtome, cut a ribbon, placed it on a glass slide, then used a Bunsen burner to fit it to the slide. HELLO, where is the processing people? Who are the technical advisors for this show? No wonder why people think they can do our jobs with depictions like that. I'M fuming. JTT ----- Original Message ----- From: To: "Edwards, R.E." Cc: ; "Breeden, Sara" Sent: Friday, May 18, 2007 7:20 AM Subject: Re: RE: [Histonet] Friday Fun Fume > When they first opened a pathology lab here (30+ yrs ago) the > histology lab was in a former bathroom. You could do gross, put it in > the processor, embed, cut, and stain just by swiveling the chair > around. And you had to go through the pathologist's office to enter > and leave the "lab"...and he was quite a bear in those days!! > > Angie Barnett, HTL(ASCP) > Grady Memorial Hospital > Pathology Department > 405/224-2258 > histology@gradymem.org > > > ----- Original Message ----- > From: "Edwards, R.E." > Date: Friday, May 18, 2007 4:17 am > Subject: RE: [Histonet] Friday Fun Fume > To: "Poteete, Jacquie A." , "Breeden, > Sara" , histonet@lists.utsouthwestern.edu > >> When I was a lad we used an electric iron as a hot plate >> and a toaster to dry the slides in, and warm up our >> meals, and >> dry our wet clothes on top of the autoclave never did me > any >> harm! >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >> Poteete,Jacquie A. >> Sent: 17 May 2007 15:44 >> To: Breeden, Sara; histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] Friday Fun Fume >> >> We're close to that. Our main IHC work area is an old shelf placed >> across the top of two open drawers. >> >> Jacquie Poteete MT(ASCP)QIHC >> Tulsa, OK >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >> Breeden,Sara >> Sent: Thursday, May 17, 2007 8:48 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Friday Fun Fume >> >> >> I'm back from hibernation and have an idea for a new end-of-week grin >> (hopefully). It's called "Can You Beat This?". Here's mine: I do >> special stains in an 18x18" "wet bar" sink using an under-counter >> refrigerator rack as my slide support. Can you beat that? Knock >> yourself out... >> >> >> >> Sally Breeden, HT(ASCP) >> >> NM Dept. of Agriculture >> >> Veterinary Diagnostic Services >> >> PO Box 700 >> >> Albuquerque, NM 87106 >> >> 505-841-2576 >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Fri May 18 18:49:15 2007 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Fri May 18 18:49:41 2007 Subject: [Histonet] Coplin Message-ID: <000101c799a7$254ba0d0$0202a8c0@HPPav2> Two nonsensical questions for late Friday night, concerning coplin jars. We got into a discussion about it this week, but I forgot to get onto Histonet Friday morning in time for the Friday trivia (though the CSI thread was good). 1. How do most people say "coplin"? - Cope lynn - Cop lynn We have people pronouncing it differently. Training difference? Difference in dialect? You say toe-may-toe, I say toe-mah-toe? Which brought us to question #2: 2. Who (maybe what) is Coplin/coplin? What nationality? That might make a difference in how the name should be pronounced. I've been looking for the origin/inventor/whatever, and I can't find anything. Thanks in advance. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 From jnocito <@t> satx.rr.com Wed May 23 18:59:33 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri May 18 18:59:29 2007 Subject: [Histonet] Can You Beat This? References: <4D14F0FC9316DD41972D5F03C070908B8F4477@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <014801c79d96$69322e20$d49eae18@yourxhtr8hvc4p> Here I go, flaming time. Are some of you seeing more techs just doing their job and that's it? I have a couple of techs that have accomplished more in their short time that they have been in the field than I did. Then, I have a couple of techs that just do enough to get by. Talking to them, documenting, over looking them for pay raises just doesn't affect them. Today, my PM supervisor and I were getting one of our VIPs ready. The molds were left in after cleaning. He pulled the trays out and smelled xylene. He called me over and I took a sniff (a big long one at that). I pulled out the cleaning alcohol and it was full of xylene. I went to dump it and spilled xylene all over me. I wasn't happy. My bigger question was why didn't the machine catch the vacuum leak? I don't know any more. I think it's time to open up Papa Joe's Bikini House of Pizza. JTT ----- Original Message ----- From: "Breeden, Sara" To: Sent: Friday, May 18, 2007 10:36 AM Subject: [Histonet] Can You Beat This? I've gotten some interesting, funny, and scary stories about Ye Olde Days. As one of my old bosses used to say, "If I'd known I was going to live this long, I'da taken better care of myself." Too bad all these stories about how we used to do things put us at risk! This should be a lesson to all the newbies out there to wear gloves, masks and question stuff, right? We shouldn't be afraid of what we do, just very cautious of what might come back to bite us. For example, one of my first jobs while training was changing the Technicon Duo - the dioxane vats. Aargh! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Fri May 18 19:08:25 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Fri May 18 19:08:32 2007 Subject: [Histonet] CSI Las Vegas In-Reply-To: <34BB307EFC9A65429BBB49E330675F7201B0AA81@LTA3VS003.ees.hhs.gov> References: <01MGQG8CI4Q88WWDSK@Macon2.Mercer.edu> <34BB307EFC9A65429BBB49E330675F7201B0AA81@LTA3VS003.ees.hhs.gov> Message-ID: <1D440703-1174-4B98-82DE-E2E58F78CDDD@yahoo.com> As I stated, Ed Brock was a technical consultant, not a script writer. He was truly dedicated to our profession. As many of you who have known Ed since the late 60's, he worked closely with the likes of lee Luna, Jules Elias, Edna Prophet and Ann Preece, contributing to continuing education for our profession. When he was with Technicon, he and Don Mayfield sponsored the 1st histology workshops with Lee Luna at AFIP. He had a love and respect for us. As anything in life, anything can be taken wrong! This is why I am most likely going to only be a observer to this website. 99% can be correct and you will get flamed for that 1% that is misconstrued. What a pity! Akemi Allison-Tacha On May 18, 2007, at 1:29 PM, Bartlett, Jeanine (CDC/CCID/NCZVED) wrote: > Good old Sam! He was supposedly a combo. histotech and pathology > assistant. > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > Shirley Powell > Sent: Fri 5/18/2007 2:25 PM > To: 'Akemi Allison-Tacha'; 'Jackie M O'Connor'; > histonet@lists.utsouthwestern.edu; histonet- > bounces@lists.utsouthwestern.edu > Cc: broed@aol.com > Subject: RE: [Histonet] CSI Las Vegas > > Akemi, > I remember watching Quincy when he told his side kick (I can't > remember his > name) that his job was going to be "reduced" to becoming a > histology tech > "receiving and labeling specimens". I quit watching that show > after that > condescending remark. Your friend Ed would have really gotten > flamed if we > had histonet then. :) > Shirley > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi > Allison-Tacha > Sent: Friday, May 18, 2007 12:00 PM > To: Jackie M O'Connor; histonet@lists.utsouthwestern.edu; > histonet-bounces@lists.utsouthwestern.edu > Cc: broed@aol.com > Subject: Re: [Histonet] CSI Las Vegas > > I didn't catch the show last night, but years ago, there was a show > called > Quincy...very similar to CSI. > My best friend Ed Brock was the technical consultant for that > show. He > worked for Technicon at that time and made sure they were atleast > close to > being on track. Times change. > > Akemi Allison-Tacha > > --- Jackie M O'Connor > wrote: > > > Anyone catch the histology scene in CSI last night? > > The medical examiner > > took a piece of skin from a victim, put it in a mold, poured wax on > > it, put it in a microtome, turned the wheel backwards to get a > section > > on a slide, melted the wax off with a Bunsen burner, then looked at > > the section under the microscope - -from that he determined that the > > victim was electrocuted. I love TV science. > > > > Happy Friday! > > > > Jackie O' > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Eric.Hoy <@t> UTSouthwestern.edu Fri May 18 20:52:05 2007 From: Eric.Hoy <@t> UTSouthwestern.edu (Eric Hoy) Date: Fri May 18 20:52:38 2007 Subject: [Histonet] CSI Las Vegas In-Reply-To: <34BB307EFC9A65429BBB49E330675F7201B0AA80@LTA3VS003.ees.hhs.gov> Message-ID: On 5/18/07 12:54 PM, "Bartlett, Jeanine (CDC/CCID/NCZVED)" wrote: > Don't forget the doctors from House taking a piece of tissue from the > refrigerator, placing it on a petri dish, dropping red stuff on it and looking > under the scope to see the "immuno".....and all of the doctors do all of the > procedures themselves, including all the lab work. > One of my favorites was on the old series "St. Elsewhere". In one episode, a nurse was shown in "the lab", digging thru a common kitchen refrigerator (I think it was Harvest Gold, a popular color in the 70's), looking for a unit of blood to administer to a patient. She had to push aside the lunches of the "lab techs" to find the blood unit. Television has gotten better, but even the consultants they use (usually MD's) don't always know what really goes on in the lab. Eric =================================================== Eric S. Hoy, Ph.D., SI(ASCP) Clinical Associate Professor Department of Medical Laboratory Sciences The University of Texas Southwestern Medical Center Dallas, Texas Email: Eric.Hoy@UTSouthwestern.edu =================================================== From rjbuesa <@t> yahoo.com Sat May 19 07:50:46 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat May 19 07:50:53 2007 Subject: [Histonet] histology supervisor with experience needed. In-Reply-To: <38961.80501.qm@web81602.mail.mud.yahoo.com> Message-ID: <863313.22736.qm@web61212.mail.yahoo.com> Salary is always a tricky proposition. If you want to be, at least fair, offer the salary range in your area. If you want to offer what your lab requires, analyze the impact of what having or not having that position filled will have on your work output. A good supervisor is your only guarantee to have a lab running smoothly and with quality. What I am getting at is that the salary should be dictated by your requirements, needs and expectatoins. Don't use other labs as a source of "inspiration", yours is the problem and yours should be the rate. Just a thought! Ren? J. curt tague wrote: i am getting really busy and find that i am sometimes ovewhelmed. my lab is growing fast and with an influx of staff i could use a little help keeping things/people in line... scheduling and the like. we're in so cal, (moving to anaheim in less than 2 mos.) do about 500 block/night and will be adding approx 800 more in the next 6-8 months. what kind of salary does that position dictate? if interested, please include you requirements. curt pathology arts --------------------------------- Need a vacation? Get great deals to amazing places on Yahoo! Travel. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- No need to miss a message. Get email on-the-go with Yahoo! Mail for Mobile. Get started. From rjbuesa <@t> yahoo.com Sat May 19 07:53:44 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat May 19 07:53:49 2007 Subject: [Histonet] Friday Fun Fume In-Reply-To: <70EEF3D43B3C164C94037D811B2BE193E129DF@VHAV20MSGA3.v20.med.va.gov> Message-ID: <589178.68235.qm@web61222.mail.yahoo.com> Judge by the title you receive, and delete if you think you are not interested, but don't project your frustration and bad maners in public. We all receive the same amount of mesages but only few, like you, complain. Or you can do as some other very "bothered" listers do: unsubscribe! Ren? J. "Wiese, Jason VHAROS" wrote: Okay, so this is like the 50th message today on this particular matter. I am a man of much reserve, but my mailbox is inundated with junk. Can we perhaps minimize the BS? Just a thought? JW -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, May 23, 2007 4:34 PM To: histology@gradymem.org; Edwards, R.E. Cc: histonet@lists.utsouthwestern.edu; Breeden, Sara Subject: Re: RE: [Histonet] Friday Fun Fume Ok, here you go. I know TV is TV but did anyone catch the CSI episode last night? The "doctor" cut a piece of skin off of a "victim, placed it a cassette, filled it with paraffin, then put it on a Lietz microtome, cut a ribbon, placed it on a glass slide, then used a Bunsen burner to fit it to the slide. HELLO, where is the processing people? Who are the technical advisors for this show? No wonder why people think they can do our jobs with depictions like that. I'M fuming. JTT ----- Original Message ----- From: To: "Edwards, R.E." Cc: ; "Breeden, Sara" Sent: Friday, May 18, 2007 7:20 AM Subject: Re: RE: [Histonet] Friday Fun Fume > When they first opened a pathology lab here (30+ yrs ago) the > histology lab was in a former bathroom. You could do gross, put it in > the processor, embed, cut, and stain just by swiveling the chair > around. And you had to go through the pathologist's office to enter > and leave the "lab"...and he was quite a bear in those days!! > > Angie Barnett, HTL(ASCP) > Grady Memorial Hospital > Pathology Department > 405/224-2258 > histology@gradymem.org > > > ----- Original Message ----- > From: "Edwards, R.E." > Date: Friday, May 18, 2007 4:17 am > Subject: RE: [Histonet] Friday Fun Fume > To: "Poteete, Jacquie A." , "Breeden, > Sara" , histonet@lists.utsouthwestern.edu > >> When I was a lad we used an electric iron as a hot plate >> and a toaster to dry the slides in, and warm up our >> meals, and >> dry our wet clothes on top of the autoclave never did me > any >> harm! >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >> Poteete,Jacquie A. >> Sent: 17 May 2007 15:44 >> To: Breeden, Sara; histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] Friday Fun Fume >> >> We're close to that. Our main IHC work area is an old shelf placed >> across the top of two open drawers. >> >> Jacquie Poteete MT(ASCP)QIHC >> Tulsa, OK >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >> Breeden,Sara >> Sent: Thursday, May 17, 2007 8:48 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Friday Fun Fume >> >> >> I'm back from hibernation and have an idea for a new end-of-week grin >> (hopefully). It's called "Can You Beat This?". Here's mine: I do >> special stains in an 18x18" "wet bar" sink using an under-counter >> refrigerator rack as my slide support. Can you beat that? Knock >> yourself out... >> >> >> >> Sally Breeden, HT(ASCP) >> >> NM Dept. of Agriculture >> >> Veterinary Diagnostic Services >> >> PO Box 700 >> >> Albuquerque, NM 87106 >> >> 505-841-2576 >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Sick sense of humor? Visit Yahoo! TV's Comedy with an Edge to see what's on, when. From rjbuesa <@t> yahoo.com Sat May 19 08:02:03 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat May 19 08:02:07 2007 Subject: [Histonet] Coplin In-Reply-To: <000101c799a7$254ba0d0$0202a8c0@HPPav2> Message-ID: <723674.18937.qm@web61215.mail.yahoo.com> I always said it Co-pleen (the same "ee" as in spleen) with verbal accent on "Co"- C?-pleen Ren? J. Lee & Peggy Wenk wrote: Two nonsensical questions for late Friday night, concerning coplin jars. We got into a discussion about it this week, but I forgot to get onto Histonet Friday morning in time for the Friday trivia (though the CSI thread was good). 1. How do most people say "coplin"? - Cope lynn - Cop lynn We have people pronouncing it differently. Training difference? Difference in dialect? You say toe-may-toe, I say toe-mah-toe? Which brought us to question #2: 2. Who (maybe what) is Coplin/coplin? What nationality? That might make a difference in how the name should be pronounced. I've been looking for the origin/inventor/whatever, and I can't find anything. Thanks in advance. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! oneSearch: Finally, mobile search that gives answers, not web links. From gu.lang <@t> gmx.at Sat May 19 12:37:03 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat May 19 12:37:12 2007 Subject: AW: [Histonet] Coplin In-Reply-To: <000101c799a7$254ba0d0$0202a8c0@HPPav2> Message-ID: <000301c79a3c$50d5e9a0$6412a8c0@dielangs.at> Inspired from the Friday-Fun mails I must admit, that I say "Kuevette" to the coplin jar. (but my mother tongue is German) I think Coplin was a person. There exist various jar-models. One is named after Coplin and one after Hellendahl. And I wouldn't be surprised if he was a French and his name is pronounced this way (like Gobelin the carpet). Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Lee & Peggy Wenk Gesendet: Samstag, 19. Mai 2007 01:49 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Coplin Two nonsensical questions for late Friday night, concerning coplin jars. We got into a discussion about it this week, but I forgot to get onto Histonet Friday morning in time for the Friday trivia (though the CSI thread was good). 1. How do most people say "coplin"? - Cope lynn - Cop lynn We have people pronouncing it differently. Training difference? Difference in dialect? You say toe-may-toe, I say toe-mah-toe? Which brought us to question #2: 2. Who (maybe what) is Coplin/coplin? What nationality? That might make a difference in how the name should be pronounced. I've been looking for the origin/inventor/whatever, and I can't find anything. Thanks in advance. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> CDC.GOV Sat May 19 13:54:21 2007 From: jqb7 <@t> CDC.GOV (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Sat May 19 13:54:55 2007 Subject: [Histonet] Coplin References: <000101c799a7$254ba0d0$0202a8c0@HPPav2> Message-ID: <34BB307EFC9A65429BBB49E330675F7201B0AA89@LTA3VS003.ees.hhs.gov> cope lynn ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Lee & Peggy Wenk Sent: Fri 5/18/2007 7:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Coplin Two nonsensical questions for late Friday night, concerning coplin jars. We got into a discussion about it this week, but I forgot to get onto Histonet Friday morning in time for the Friday trivia (though the CSI thread was good). 1. How do most people say "coplin"? - Cope lynn - Cop lynn We have people pronouncing it differently. Training difference? Difference in dialect? You say toe-may-toe, I say toe-mah-toe? Which brought us to question #2: 2. Who (maybe what) is Coplin/coplin? What nationality? That might make a difference in how the name should be pronounced. I've been looking for the origin/inventor/whatever, and I can't find anything. Thanks in advance. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Djemge <@t> aol.com Sat May 19 16:43:45 2007 From: Djemge <@t> aol.com (Djemge@aol.com) Date: Sat May 19 16:43:58 2007 Subject: [Histonet] re: Friday Fun Fume Message-ID: JW the histonet list server gives you the option of receiving batch digests. By batching all the messages you only get a couple of e-mails a day that includes all the topics and discussions. This has worked better for me than receiving an in box full of every single post. Someone on the histonet should be able to tell you how to make this switch to a batch digest. Donna Emge, HT(ASCP) Northwestern University 303 E. Superior, Lurie 7-220 312-503-2036 _d-emge@northwestern.edu_ (mailto:d-emge@northwestern.edu) djemge@aol.com ************************************** See what's free at http://www.aol.com. From galalmkh <@t> yahoo.com Sun May 20 07:46:56 2007 From: galalmkh <@t> yahoo.com (manal galal) Date: Sun May 20 07:47:10 2007 Subject: [Histonet] Re: Histonet Digest, Vol 42, Issue 26 Message-ID: <76774.34233.qm@web50205.mail.re2.yahoo.com> Hi, I do special satins on frozen muscle sections sections. I air dry them for about one hour and never fix them. try it and tell me how it works out. histonet-request@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Can you top this? (Margaret Horne) 2. Please remove (mjlco@aol.com) 3. frozen special stains (Ingles Claire) 4. Help with Tunel (Michael J. Lyon, Ph.D.) 5. Flex Alcohols (Marsh, Nannette) 6. Re: Processing (Joe Nocito) 7. RE: Friday Fun Fume (Edwards, R.E.) 8. Re: RE: [Histonet] Friday Fun Fume (histology@gradymem.org) 9. Re: RE: [Histonet] Friday Fun Fume (Lynette Pavelich) 10. RE: RE: [Histonet] Friday Fun Fume (Shirley Powell) 11. RE: [SPAM] Re: RE: [Histonet] Friday Fun Fume (Mickie Johnson) 12. Can You Beat This? (Breeden, Sara) 13. CSI Las Vegas (Jackie M O'Connor) 14. RE: CSI Las Vegas (Douglas D Deltour) 15. RE: Can You Beat This? (Weems, Joyce) 16. Re: CSI Las Vegas (Akemi Allison-Tacha) 17. RE: CSI Las Vegas (Shirley Powell) 18. Re: CSI Las Vegas (Thomas Pier) ---------------------------------------------------------------------- Message: 1 Date: Thu, 17 May 2007 14:45:23 -0400 From: "Margaret Horne" Subject: [Histonet] Can you top this? To: Message-ID: <464C6A83.23366.88628D@localhost> Content-Type: text/plain; charset=US-ASCII I keep my diluted antibodies in shell vials but needed a way to keep them organised, so : I got a Ferrraro Roche container from a friend ( clear plastic , rectangular) and the cardboard divider from a - 80 freezer box. Cut the divider to fit and organised the vials in their serial dilutions. Fits beautifully in the door of the fridge. A second similar container holds my small bottles of streptavidin and my Biotinylated secondary, etc. My friend thinks I should get another box of those chocolates and charge it to my boss. I haven't had the nerve. My incubation chambers for IHC are rubbermaid containers. For TEM IHC I got two ceramic escargo dishes, each has 6 wells, for $2 in a grocery store sale bin. I was tickled as the ones from an EM catalogue are about $15 each. But my favourite is the wooden stir stick with a eyelash attached to the end by a dab of nail polish that I use with my $3000 diamond knife to do TEM work. The incongruity always makes me chuckle. I like this thread. It's more than a good chuckle. It's an exchange of neat ideas. thanks , margaret Margaret Horne , Histology Teaching Assistant, Dept. of B.SC., Atlantic Veterinary College, U.P.E.I., 550 University Ave., Charlottetown, P.E.I., C1A 4P3 Canada ------------------------------ Message: 2 Date: Thu, 17 May 2007 14:49:51 -0400 From: mjlco@aol.com Subject: [Histonet] Please remove To: histonet@lists.utsouthwestern.edu Message-ID: <8C966CB491EAE11-16D8-1665@webmail-db17.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" I am off on holiday and dont want to fill up my box. Thanks, matt Longmont united hospital ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. ------------------------------ Message: 3 Date: Thu, 17 May 2007 14:42:28 -0500 From: "Ingles Claire" Subject: [Histonet] frozen special stains To: Message-ID: <08A0A863637F1349BBFD83A96B27A50A120042@uwhis-xchng3.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="iso-8859-1" Hello out there! I have a question for the collective histo-brain out there. (resistance is futile). Does anyone do special stains i.e. trichrome and/or elastic stains on frozen tissue (skin)? I have been trying these out using the regular protocols you would use for FFPE tissues. The (FFPE) controls I stain with the frozens come out fine, but the staining of the frozen sections is so intense that it is difficult to interpret. On the trichrome, the epidermis is so dark it looks black. I've tried cutting the incubation times back by as much as half. It helps a bit but is still pretty intense. Are there any special procedures out there to make them turn out more normal looking? For procedure, I cut sections at 5 microns, dry in a 60 oven for 10 min, and fix in 10% formalin for 30 min. Then rinse in running tap for about 5 minutes and perform stain as normal. Help?!? Claire Ingles UW Hospital Madison WI ------------------------------ Message: 4 Date: Thu, 17 May 2007 15:37:09 -0400 From: "Michael J. Lyon, Ph.D." Subject: [Histonet] Help with Tunel To: Message-ID: <004a01c798ba$c2a571f0$31ae7f8b@lyonoffice> Content-Type: text/plain; charset="us-ascii" I am looking for some help using the Chemicon ApopTag kit. We have used this successfully in the past on formalin fixed human muscle, frozen sections. We are looking at a different human muscle, processed similarly. However our results are incredibly inconsistent even on the positive control DNAse treated sections of the same muscle. Some of the sections have nothing at all. While other sections have areas of very bright nuclei and other areas are nearly negative. Any help appreciated. Thanks Michael J. Lyon, Ph.D. Otolaryngology Research Lab SUNY Upstate Medical University 750 East Adams Street Syracuse, NY 13210 Voice 315-464-7253 Fax 315-464-5572 ------------------------------ Message: 5 Date: Thu, 17 May 2007 15:30:37 -0500 From: "Marsh, Nannette" Subject: [Histonet] Flex Alcohols To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hello. We are considering switching to the Flex alcohols produced by Richard Allen. Any comments, especially from research personnel are greatly appreciated. Thanks, Nanne Nanne Marsh Histology Specialist I Stowers Institute for Medical Research ------------------------------ Message: 6 Date: Tue, 22 May 2007 18:09:49 -0500 From: "Joe Nocito" Subject: Re: [Histonet] Processing To: "Douglas D Deltour" , "'Jackie M O'Connor'" , "'Coco'" Cc: Histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: <015a01c79cc6$4ef49d20$d49eae18@yourxhtr8hvc4p> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Heather, have you tried talking to Civilian Personnel? I had a GS7 who was a force-hire because another base closed due to BRAC. You talk about a nightmare! Freddy Kruger had nothing on this woman. Civilian Personnel was a saving grace. JTT ----- Original Message ----- From: "Douglas D Deltour" To: "'Jackie M O'Connor'" ; "'Coco'" Cc: ; Sent: Wednesday, May 16, 2007 1:54 PM Subject: RE: [Histonet] Processing >I agree with Jackie on this Heather. Having worked in an exact environment > that you work in (A Navy Hospital) I can tell you that the military and > the > GS have a totally different mindset than the "real world". I did it for 14 > years (I won't tell you which side). Just face up to it because you will > always have some sort of a military vs. GS conflict. You can try to rattle > the military paths cage but to him you will be "the civilian" and if you > make too many waves then you will be a "trouble making civilian". > It sounds like you do not have a very supportive GS chain at your > hospital. > Most likely your GS boss is on the clinical side and considers the AP side > the "stepchild" of the lab. Maybe he/she lacks the backbone to support > you. > It is all about getting through another day without any bother for most. > > As for the GS PD... I bet you and your worker have a little sentence at > the > end of it that reads "All other duties assigned", or something like it. > > The GS pay system is below standard and the benefits are not what they > used > to be. Is it really worth the headache? > > No offense to the GS workers out there. I appreciate your tolerance. > > > Douglas D. Deltour HT(ASCP) > Histology Manager > Professional Pathology Services, PC > One Science Court > Suite 200 > Columbia, SC 29203 > (803)252-1913 > Fax (803)254-3262 > > ***************************************************** > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of the individual or entity to > which it is addressed and may contain information that is privileged, > confidential and exempt from disclosure under applicable law. If the > reader > of this message is not the intended recipient, you are hereby notified > that > any dissemination, distribution, or copying of this communication is > strictly prohibited by law. If you have received this communication in > error, please notify me immediately. > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M > O'Connor > Sent: Wednesday, May 16, 2007 7:44 AM > To: Coco > Cc: Histonet@lists.utsouthwestern.edu; > histonet-bounces@lists.utsouthwestern.edu > Subject: Re: [Histonet] Processing > > Heather - > If you're not active duty, why don't you get out of that job? Seems like > you've been struggling a long time there - -maybe that job just isn't the > right fit. > Best wishes, > Jackie > > > > "Coco" > Sent by: histonet-bounces@lists.utsouthwestern.edu > 05/15/2007 06:13 PM > > To > > cc > > Subject > [Histonet] Processing > > > > > > > Well anybody who is a GS, if you know a friend of a friend, they will > get > you a job. I agree military believe that civilians are enlisted people, > and > think that you can be cross trained in every department, like a corpsman. > Unfortunately, that is why we have work pd's. Civilian vs. military is not > a > great interface. A lot of problems are not dealt with until they directly > affect that person. Anyways, to the person who said, "report it to CAP". > What will they do? At this point, I wish it was easy to say that this > person > could label slides. The accessioning process after 60 days is a nightmare. > I've repeated, visually shown, written things down and this lady is still > struggling. But for those who are not GS. A GS 7 can not train a GS 7, nor > the secretary at GS 5, can not train a GS 7. The work PD does not state > OJT. > So right now there is a major violation going on. This lady just happened > to > want a GS job so badly, she might have sold her soul to the devil to get > it > and now she is paying dearly. So she is on a year probation, and I'm > between > a rock and a hard place because she is not capable of performing the job. > I > have already rattled the pathologists cage, and I am getting ignored. I'm > just as guilty of standing by and doing nothing when patient care is being > compromised and when there is a problem, military come after the civilian > and pin the blame on you. That is why the BS is unreal and very very > political. I am debating whether to tell the CO. What should I do? That is > a > trickle down effect and there will be retaliation. Anybody have any ideas > on > what I should do? Let it go, or go to the top and than commanders will > take > some serious heat. Regardless oh how this situation is dealt with the > nending is not going to be nice. I don't want this woman to lose her job > but > than again she is incompetent. All opinions appreciated. > > > > Heather > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Fri, 18 May 2007 10:10:07 +0100 From: "Edwards, R.E." Subject: RE: [Histonet] Friday Fun Fume To: "Poteete, Jacquie A." , "Breeden, Sara" , Message-ID: Content-Type: text/plain; charset="US-ASCII" When I was a lad we used an electric iron as a hot plate and a toaster to dry the slides in, and warm up our meals, and dry our wet clothes on top of the autoclave never did me any harm! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Poteete, Jacquie A. Sent: 17 May 2007 15:44 To: Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Friday Fun Fume We're close to that. Our main IHC work area is an old shelf placed across the top of two open drawers. Jacquie Poteete MT(ASCP)QIHC Tulsa, OK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, May 17, 2007 8:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Friday Fun Fume I'm back from hibernation and have an idea for a new end-of-week grin (hopefully). It's called "Can You Beat This?". Here's mine: I do special stains in an 18x18" "wet bar" sink using an under-counter refrigerator rack as my slide support. Can you beat that? Knock yourself out... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Fri, 18 May 2007 07:20:01 -0500 From: histology@gradymem.org Subject: Re: RE: [Histonet] Friday Fun Fume To: "Edwards, R.E." Cc: histonet@lists.utsouthwestern.edu, "Breeden, Sara" Message-ID: Content-Type: text/plain; charset=us-ascii When they first opened a pathology lab here (30+ yrs ago) the histology lab was in a former bathroom. You could do gross, put it in the processor, embed, cut, and stain just by swiveling the chair around. And you had to go through the pathologist's office to enter and leave the "lab"...and he was quite a bear in those days!! Angie Barnett, HTL(ASCP) Grady Memorial Hospital Pathology Department 405/224-2258 histology@gradymem.org ----- Original Message ----- From: "Edwards, R.E." Date: Friday, May 18, 2007 4:17 am Subject: RE: [Histonet] Friday Fun Fume To: "Poteete, Jacquie A." , "Breeden, Sara" , histonet@lists.utsouthwestern.edu > When I was a lad we used an electric iron as a hot plate > and a toaster to dry the slides in, and warm up our > meals, and > dry our wet clothes on top of the autoclave never did me any > harm! > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Poteete,Jacquie A. > Sent: 17 May 2007 15:44 > To: Breeden, Sara; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Friday Fun Fume > > We're close to that. Our main IHC work area is an old shelf placed > across the top of two open drawers. > > Jacquie Poteete MT(ASCP)QIHC > Tulsa, OK > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Breeden,Sara > Sent: Thursday, May 17, 2007 8:48 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Friday Fun Fume > > > I'm back from hibernation and have an idea for a new end-of-week grin > (hopefully). It's called "Can You Beat This?". Here's mine: I do > special stains in an 18x18" "wet bar" sink using an under-counter > refrigerator rack as my slide support. Can you beat that? Knock > yourself out... > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 9 Date: Fri, 18 May 2007 09:25:24 -0400 From: "Lynette Pavelich" Subject: Re: RE: [Histonet] Friday Fun Fume To: , Cc: histonet@lists.utsouthwestern.edu, sbreeden@nmda.nmsu.edu Message-ID: <464D7105020000EE00014376@smtp-gw.hurleymc.com> Content-Type: text/plain; charset=US-ASCII Back in '71, when I was a student, we were in 1 room with 2 windows on the same side of the building so there was no cross venilation. The box fan was propped up in the window in front of the docs when they grossed blowing out, winter or summer. I still remember going home so "high" from the xylene fumes that it took about 30 minutes to come around. Never remembered driving home and if I ever went through a red light or not!!! Some guardian angel, huh!! Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810-257-9948 fax: 810-762-7082 ------------------------------ Message: 10 Date: Fri, 18 May 2007 10:29:22 -0400 From: Shirley Powell Subject: RE: RE: [Histonet] Friday Fun Fume To: 'Lynette Pavelich' , histology@gradymem.org, ree3@leicester.ac.uk Cc: histonet@lists.utsouthwestern.edu, sbreeden@nmda.nmsu.edu Message-ID: <01MGQ7YQSQBK8WWLGQ@Macon2.Mercer.edu> Content-Type: text/plain; charset=us-ascii 45 years ago (coming this July 18th) when I began training (I was 2 at the time :) ), it was in the basement of the hospital in the animal room where they used to keep the rabbits for the pregnancy tests, no windows, not much ventilation if any, and we dried our slides with a hair dryer (yes they had those back then) and we stored all the specimens in jars of formalin in the closet next to that room, so now you know why I am defective, or is it just fixed. Xylene fumes, I can't smell anymore and my liver enzymes run high, not me. Oh well that could be from the wine consumption. My salary at that time would not buy groceries today for a week and gas is out of the question. Call me an antique, not old. Shirley Powell -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Friday, May 18, 2007 9:25 AM To: histology@gradymem.org; ree3@leicester.ac.uk Cc: histonet@lists.utsouthwestern.edu; sbreeden@nmda.nmsu.edu Subject: Re: RE: [Histonet] Friday Fun Fume Back in '71, when I was a student, we were in 1 room with 2 windows on the same side of the building so there was no cross venilation. The box fan was === message truncated === --------------------------------- Park yourself in front of a world of choices in alternative vehicles. Visit the Yahoo! Auto Green Center. From galalmkh <@t> yahoo.com Sun May 20 08:03:41 2007 From: galalmkh <@t> yahoo.com (manal galal) Date: Sun May 20 08:03:49 2007 Subject: [Histonet] Re: tissue crumbled frozen sections Message-ID: <601200.73340.qm@web50210.mail.re2.yahoo.com> Hi, I do all my work on frozen muscle and I'm proud to say I have practically no artifacts or crumbling. I freeze in the cryostat at -40c . I recieve the muscle immediately while the patient is still on the table and it is always put in gauze dampened with saline ( otherwise I get lots of artfacts). Dr. Manal Galal MD pathology Cairo, Egypt histonet-request@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Crumbling frozen sections (Mike Pence) ---------------------------------------------------------------------- Message: 1 Date: Thu, 10 May 2007 11:49:56 -0500 From: "Mike Pence" Subject: RE: [Histonet] Crumbling frozen sections To: "Martin S." , "Gayle Callis" Cc: histonet@lists.utsouthwestern.edu Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C5C8@IS-E2K3.grhs.net> Content-Type: text/plain; charset="us-ascii" The difference you have is snap frozen tissue vs. tissue frozen by dry ice and isopentane freezing. You are more than likely getting freeze artifact from the tissue setting around in a closed tube (moisture build-up and ice crystal formation when frozen with iso.) Try handling your colorectal tissues in the same manner and see what happens. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin S. Sent: Thursday, May 10, 2007 11:25 AM To: Gayle Callis Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Crumbling frozen sections I have tried a range of temps from -30oC to -10 (I went up in 5oC intervals). For the colorectal tumour that was snap frozen cutting at -20oC was good its just these new ones that I'm having a problem with. The tissues are put in a plastic tube without anything else - just a blob of tissue. I'm not sure how long the tissue sits like this before I get it - the surgeon isnt very forthcoming with info! I have been using APES coated slides (which I coat myself 5% APES in Acetone, 5min, wash dH2O x3, dried in oven) and have done one test with VWR Superfrost charged slides (no difference). Thanks Sonya -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: 10 May 2007 15:55 To: Martin S. Subject: Re: [Histonet] Crumbling frozen sections Have you played with cryostat temperature to improve sectioning? It may be too cold (you did not say what temp you cut at?). Are the human tissues coming to you fresh or have they been dunked into formalin? Do they put this on saline moistened guaze to keep it from drying out? Also, what slides are you putting the tissues on, plus charge? So many questions? At 08:19 AM 5/10/2007, you wrote: >Hi All, > >I have been doing a lot of frozen sectioning of mouse tissues. I remove >the organ (spleen, lymph nodes, liver etc) from the mouse immerse it in >OCT in a foil mould and then place in a bath of isopentane on dry ice. >This has been working really well and I have been getting good sections. > >I am now looking at some human tissue (colo-rectal and liver tumours). >I receive a small piece of tissue from the surgeon which I have been >treating as for the mouse tissue. However I am finding it very hard to >get good sections. The tissue seems very flaky and crumbly and either >disintegrates on cutting or if I do get what looks like an ok section >by the time I have gone through the staining procedure it has >completely gone! > >I am not sure how long it takes between the tissue being removed and me >getting the sample - maybe 30min - could this be the cause? > >I have some old colo-rectal tumours that were snap frozen in liquid >nitrogen and they seem much better - I think I'll try doing this from >now on but just wondering if anyone has any insight into why I'm having >such problems. > >Thanks! > >Sonya > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 42, Issue 15 **************************************** --------------------------------- We won't tell. Get more on shows you hate to love (and love to hate): Yahoo! TV's Guilty Pleasures list. From Dorothy.L.Webb <@t> HealthPartners.Com Sun May 20 11:28:15 2007 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Sun May 20 11:28:22 2007 Subject: [Histonet] Microwave processing Message-ID: <0E394B648E5284478A6CCB78E5AFDA2703640CC3@hpes1.HealthPartners.int> For those of your who do microwave processing, have you ever had a problem with one run where the slides look terrible (smudgy, poor nuclear detail, uneven staining) and the previous and next run were fine?? What could cause this to happen? If you would like to Email me personally or call me at 651-254-2962, I would greatly appreciate it, for I am stymied!! Thanks, ahead of time to all the great histonetters..what an awesome group!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From sbreeden <@t> nmda.nmsu.edu Sun May 20 12:41:22 2007 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Sun May 20 12:41:31 2007 Subject: [Histonet] Friday Fume Runover Message-ID: <4D14F0FC9316DD41972D5F03C070908B8F4478@nmdamailsvr.nmda.ad.nmsu.edu> All this chat of CSI - which is a good thing, even though some believe this chat to be spam - brings me (thankfully) to my point: Why don't we (or me, or one of us, or someone with years of visibility in this profession) contact the producers of these shows and drop the big hint that with histology technicians in particular being in such demand, the benefit of showing how we actually do our work could have a beneficial side effect of generating some interest in our field. "Kids" watch these shows (NCIS, CSI, etc.) for the goo and ooze, but showing a short REALISTIC (I'm not shouting) histology segment might just light some bulbs in some of these "kids". I know...I know... but you never know until you try. The interest that's been generated by such shows could be absolutely put to good use! Here in New Mexico (that's that State between ARIZONA and TEXAS [this might be shouting...]), our educational system is investigating "career pathways" beginning in mid-high school; the plan is to determine interest and ability and then allow a focus to the student's education. I was asked to join the "Healthcare Pipeline" portion of the Governor's Task Force after I began searching for a way to get the histology subject in front of mid-high and high-school students. The members (mostly nurses) asked for a synopsis of what histologists do and most had little or no knowledge of our profession. I have even managed to do the "chamber of commerce" sales pitch for histology on one of the vet tech students that rotate through our facility and she just began an informal OJT course with me. The interest is out there - we just have to cultivate it! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 From rjbuesa <@t> yahoo.com Sun May 20 13:06:16 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun May 20 13:06:20 2007 Subject: [Histonet] Friday Fume Runover In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B8F4478@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <428235.61171.qm@web61222.mail.yahoo.com> Sara: Vinnie de la Esperanza, as President of NSH should be the one in charge of that contact. I am forwarding him your e-mail Ren? J. "Breeden, Sara" wrote: All this chat of CSI - which is a good thing, even though some believe this chat to be spam - brings me (thankfully) to my point: Why don't we (or me, or one of us, or someone with years of visibility in this profession) contact the producers of these shows and drop the big hint that with histology technicians in particular being in such demand, the benefit of showing how we actually do our work could have a beneficial side effect of generating some interest in our field. "Kids" watch these shows (NCIS, CSI, etc.) for the goo and ooze, but showing a short REALISTIC (I'm not shouting) histology segment might just light some bulbs in some of these "kids". I know...I know... but you never know until you try. The interest that's been generated by such shows could be absolutely put to good use! Here in New Mexico (that's that State between ARIZONA and TEXAS [this might be shouting...]), our educational system is investigating "career pathways" beginning in mid-high school; the plan is to determine interest and ability and then allow a focus to the student's education. I was asked to join the "Healthcare Pipeline" portion of the Governor's Task Force after I began searching for a way to get the histology subject in front of mid-high and high-school students. The members (mostly nurses) asked for a synopsis of what histologists do and most had little or no knowledge of our profession. I have even managed to do the "chamber of commerce" sales pitch for histology on one of the vet tech students that rotate through our facility and she just began an informal OJT course with me. The interest is out there - we just have to cultivate it! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Got a little couch potato? Check out fun summer activities for kids. From rjbuesa <@t> yahoo.com Sun May 20 13:11:24 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun May 20 13:11:27 2007 Subject: [Histonet] Another question! Message-ID: <332113.64123.qm@web61219.mail.yahoo.com> Dear Colleagues: By now you already know that from time to time I come up with a general question, here is another one: Are we histotechs ARTISTS (creating new beautiful and original things daily) or are we ARTISANS assuring that our work responds to certain established norms in a most beautiful way? It would be nice to know how we, as a collective, feel about this questions: artists or artisans? Ren? J. --------------------------------- Building a website is a piece of cake. Yahoo! Small Business gives you all the tools to get online. From histotechkb <@t> gmail.com Sun May 20 14:47:31 2007 From: histotechkb <@t> gmail.com (Kristen Broomall) Date: Sun May 20 14:47:44 2007 Subject: [Histonet] Coplin In-Reply-To: <34BB307EFC9A65429BBB49E330675F7201B0AA89@LTA3VS003.ees.hhs.gov> References: <000101c799a7$254ba0d0$0202a8c0@HPPav2> <34BB307EFC9A65429BBB49E330675F7201B0AA89@LTA3VS003.ees.hhs.gov> Message-ID: <667c97ab0705201247i59d260vef184df709271f90@mail.gmail.com> Cope lynn here too On 5/19/07, Bartlett, Jeanine (CDC/CCID/NCZVED) wrote: > > cope lynn > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Lee & Peggy > Wenk > Sent: Fri 5/18/2007 7:49 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Coplin > > > > Two nonsensical questions for late Friday night, concerning coplin jars. > We > got into a discussion about it this week, but I forgot to get onto > Histonet > Friday morning in time for the Friday trivia (though the CSI thread was > good). > > 1. How do most people say "coplin"? > - Cope lynn > - Cop lynn > > We have people pronouncing it differently. Training difference? Difference > in dialect? You say toe-may-toe, I say toe-mah-toe? > > Which brought us to question #2: > > 2. Who (maybe what) is Coplin/coplin? What nationality? That might make a > difference in how the name should be pronounced. I've been looking for the > origin/inventor/whatever, and I can't find anything. > > Thanks in advance. > > Peggy A. Wenk, HTL(ASCP)SLS > William Beaumont Hospital > Royal Oak, MI 48073 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Kristen Broomall, HT (ASCP) Histotechnology Society of Delaware Correspondence Secretary and Newsletter Co-Editor histotechkb@gmail.com From JWEEMS <@t> sjha.org Sun May 20 16:23:21 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Sun May 20 16:23:49 2007 Subject: [Histonet] Coplin In-Reply-To: <000101c799a7$254ba0d0$0202a8c0@HPPav2> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FF992D@sjhaexc02.sjha.org> I say....Cope land. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Friday, May 18, 2007 7:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Coplin Two nonsensical questions for late Friday night, concerning coplin jars. We got into a discussion about it this week, but I forgot to get onto Histonet Friday morning in time for the Friday trivia (though the CSI thread was good). 1. How do most people say "coplin"? - Cope lynn - Cop lynn We have people pronouncing it differently. Training difference? Difference in dialect? You say toe-may-toe, I say toe-mah-toe? Which brought us to question #2: 2. Who (maybe what) is Coplin/coplin? What nationality? That might make a difference in how the name should be pronounced. I've been looking for the origin/inventor/whatever, and I can't find anything. Thanks in advance. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From cstone88 <@t> verizon.net Sun May 20 17:12:35 2007 From: cstone88 <@t> verizon.net (Cynthia Stone) Date: Sun May 20 17:13:06 2007 Subject: [Histonet] Coplin References: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FF992D@sjhaexc02.sjha.org> Message-ID: <001b01c79b2b$f8fa4a00$0201a8c0@puter> I say Cop lin, like Copley Square in Boston. ----- Original Message ----- From: "Weems, Joyce" To: ; Sent: Sunday, May 20, 2007 5:23 PM Subject: RE: [Histonet] Coplin I say....Cope land. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Friday, May 18, 2007 7:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Coplin Two nonsensical questions for late Friday night, concerning coplin jars. We got into a discussion about it this week, but I forgot to get onto Histonet Friday morning in time for the Friday trivia (though the CSI thread was good). 1. How do most people say "coplin"? - Cope lynn - Cop lynn We have people pronouncing it differently. Training difference? Difference in dialect? You say toe-may-toe, I say toe-mah-toe? Which brought us to question #2: 2. Who (maybe what) is Coplin/coplin? What nationality? That might make a difference in how the name should be pronounced. I've been looking for the origin/inventor/whatever, and I can't find anything. Thanks in advance. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Sun May 20 19:47:35 2007 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sun May 20 19:47:42 2007 Subject: [Histonet] CSI Las Vegas Message-ID: <582736990705201747p4aa89b4ao7e3c5d2511be43f5@mail.gmail.com> Denise, That sounds familiar. I was told by a researcher that he didn't want any slides cut for his tissue he only wanted wax poured on it. Really got me thinking though, How literal should I take this? Could I use a nice lavendar from Yankee Candle? Amos -------------------------------------------------- I actually caught a Cardiology Fellow trying to embed mouse heart tissue from formalin into paraffin. He wanted me to tell him what to do with the formalin when he was finished. He said he was trying to save the department research money by skipping the processing step. This was in a major Boston institution labeled as within the "top 3" in the country! Takes all kinds......... TGIF and start of my VACATION! Yipee! From jnocito <@t> satx.rr.com Fri May 25 20:59:31 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Sun May 20 20:59:30 2007 Subject: [Histonet] Another question! References: <332113.64123.qm@web61219.mail.yahoo.com> Message-ID: <005501c79f39$80d23300$d49eae18@yourxhtr8hvc4p> Artists of course. Every block is a new item, every special stain is a new canvas, every immuno is a work of art in itself. I have always thought that we, as histo techs, we most artists than anything else. No matter how much companies may automate histology, someone still has to put that section on the slide. When things go wrong, someone still has to trouble shoot. That's my 4 cents. JTT ----- Original Message ----- From: "Rene J Buesa" To: Sent: Sunday, May 20, 2007 1:11 PM Subject: [Histonet] Another question! > Dear Colleagues: > By now you already know that from time to time I come up with a general > question, here is another one: > > Are we histotechs ARTISTS (creating new beautiful and original things > daily) or are we ARTISANS assuring that our work responds to certain > established norms in a most beautiful way? > > It would be nice to know how we, as a collective, feel about this > questions: artists or artisans? > > Ren? J. > > > > > --------------------------------- > Building a website is a piece of cake. > Yahoo! Small Business gives you all the tools to get online. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From derekejohnson <@t> hotmail.com Sun May 20 23:44:48 2007 From: derekejohnson <@t> hotmail.com (Derek Johnson) Date: Sun May 20 23:44:57 2007 Subject: [Histonet] Lab Procedures Message-ID: Hello All- I am a histotechnology student and have a few questions I would like to ask this great community about your lab procedures. I don't want to be too intrusive, but any info you can give would be wonderful! Without further ado, here are my questions: 1) Who decides the format and required components of your lab's procedures, an accrediting organization or internal laboratory management? 2) About how many histotechnology related procedures exist in your laboratory? Where are they kept? 3) Who is responsible for creating new lab procedures? Who is responsible for reviewing your histology lab procedures? How often is it required to be done? 4) What is the method you use to train new personnel in your lab? Thanks for all your input! -Derek _________________________________________________________________ PC Magazine’s 2007 editors’ choice for best Web mail—award-winning Windows Live Hotmail. http://imagine-windowslive.com/hotmail/?locale=en-us&ocid=TXT_TAGHM_migration_HM_mini_pcmag_0507 From JMacDonald <@t> mtsac.edu Sun May 20 23:49:56 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Sun May 20 23:50:01 2007 Subject: [Histonet] Coplin In-Reply-To: <001b01c79b2b$f8fa4a00$0201a8c0@puter> Message-ID: I was taught cope lynn. I went to school in Canada. Any difference? Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu "Cynthia Stone" Sent by: histonet-bounces@lists.utsouthwestern.edu 05/20/2007 03:12 PM Please respond to Cynthia Stone To cc Subject Re: [Histonet] Coplin I say Cop lin, like Copley Square in Boston. ----- Original Message ----- From: "Weems, Joyce" To: ; Sent: Sunday, May 20, 2007 5:23 PM Subject: RE: [Histonet] Coplin I say....Cope land. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Friday, May 18, 2007 7:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Coplin Two nonsensical questions for late Friday night, concerning coplin jars. We got into a discussion about it this week, but I forgot to get onto Histonet Friday morning in time for the Friday trivia (though the CSI thread was good). 1. How do most people say "coplin"? - Cope lynn - Cop lynn We have people pronouncing it differently. Training difference? Difference in dialect? You say toe-may-toe, I say toe-mah-toe? Which brought us to question #2: 2. Who (maybe what) is Coplin/coplin? What nationality? That might make a difference in how the name should be pronounced. I've been looking for the origin/inventor/whatever, and I can't find anything. Thanks in advance. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Sun May 20 23:54:11 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Sun May 20 23:54:16 2007 Subject: [Histonet] Friday Fume Runover In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B8F4478@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: Sara, I actually contacted the CSI people and spoke directly with David Berman. I invited him out to the college to visit our histology lab and learn about the field, but he seemed less than interested. He loaned me a CSI tape and called it a day. Jennifer Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu "Breeden, Sara" Sent by: histonet-bounces@lists.utsouthwestern.edu 05/20/2007 10:41 AM To cc Subject [Histonet] Friday Fume Runover All this chat of CSI - which is a good thing, even though some believe this chat to be spam - brings me (thankfully) to my point: Why don't we (or me, or one of us, or someone with years of visibility in this profession) contact the producers of these shows and drop the big hint that with histology technicians in particular being in such demand, the benefit of showing how we actually do our work could have a beneficial side effect of generating some interest in our field. "Kids" watch these shows (NCIS, CSI, etc.) for the goo and ooze, but showing a short REALISTIC (I'm not shouting) histology segment might just light some bulbs in some of these "kids". I know...I know... but you never know until you try. The interest that's been generated by such shows could be absolutely put to good use! Here in New Mexico (that's that State between ARIZONA and TEXAS [this might be shouting...]), our educational system is investigating "career pathways" beginning in mid-high school; the plan is to determine interest and ability and then allow a focus to the student's education. I was asked to join the "Healthcare Pipeline" portion of the Governor's Task Force after I began searching for a way to get the histology subject in front of mid-high and high-school students. The members (mostly nurses) asked for a synopsis of what histologists do and most had little or no knowledge of our profession. I have even managed to do the "chamber of commerce" sales pitch for histology on one of the vet tech students that rotate through our facility and she just began an informal OJT course with me. The interest is out there - we just have to cultivate it! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Mon May 21 08:14:01 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon May 21 08:14:15 2007 Subject: [Histonet] CSI Las Vegas In-Reply-To: <504527.154.qm@web31303.mail.mud.yahoo.com> Message-ID: <000001c79ba9$e93f8160$d00f7ca5@lurie.northwestern.edu> At least "Bones" has been a little more accurate. They actually cut on a microtome (the right way) but I did question the fact that they never mentioned wether the bone they were cutting was decalled or not. Did anyone see S.O.B.? They did do it right. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Friday, May 18, 2007 10:00 AM To: Jackie M O'Connor; histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Cc: broed@aol.com Subject: Re: [Histonet] CSI Las Vegas I didn't catch the show last night, but years ago, there was a show called Quincy...very similar to CSI. My best friend Ed Brock was the technical consultant for that show. He worked for Technicon at that time and made sure they were atleast close to being on track. Times change. Akemi Allison-Tacha --- Jackie M O'Connor wrote: > Anyone catch the histology scene in CSI last night? > The medical examiner > took a piece of skin from a victim, put it in a > mold, poured wax on it, > put it in a microtome, turned the wheel backwards to > get a section on a > slide, melted the wax off with a Bunsen burner, then > looked at the section > under the microscope - -from that he determined that > the victim was > electrocuted. I love TV science. > > Happy Friday! > > Jackie O' > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon May 21 08:17:18 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon May 21 08:17:22 2007 Subject: [Histonet] Lab Procedures In-Reply-To: Message-ID: <755548.97442.qm@web61219.mail.yahoo.com> Derek: Short answers to your questions: 1- NCLSS originally dictated the formate; 2- About 40 all in the SOP (Standard Operating Procedures); 3-The Supervisor selects new procedures and if s/he knows how, could "create" new ones, to be "blind tested" and approved by the lab director; and 4- The same as going to Carnegie Hall: "practice, practice, practice" Ren? J. Derek Johnson wrote: Hello All- I am a histotechnology student and have a few questions I would like to ask this great community about your lab procedures. I don't want to be too intrusive, but any info you can give would be wonderful! Without further ado, here are my questions: 1) Who decides the format and required components of your lab's procedures, an accrediting organization or internal laboratory management? 2) About how many histotechnology related procedures exist in your laboratory? Where are they kept? 3) Who is responsible for creating new lab procedures? Who is responsible for reviewing your histology lab procedures? How often is it required to be done? 4) What is the method you use to train new personnel in your lab? Thanks for all your input! -Derek _________________________________________________________________ PC Magazine?s 2007 editors? choice for best Web mail?award-winning Windows Live Hotmail. http://imagine-windowslive.com/hotmail/?locale=en-us&ocid=TXT_TAGHM_migration_HM_mini_pcmag_0507 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Give spam the boot. Take control with tough spam protection in the all-new Yahoo! Mail Beta. From pmcardle <@t> ebsciences.com Mon May 21 08:32:07 2007 From: pmcardle <@t> ebsciences.com (Phil McArdle) Date: Mon May 21 08:32:18 2007 Subject: [Histonet] Microwave processing In-Reply-To: <0E394B648E5284478A6CCB78E5AFDA2703640CC3@hpes1.HealthPartners.int> References: <0E394B648E5284478A6CCB78E5AFDA2703640CC3@hpes1.HealthPartners.int> Message-ID: <46519F57.4000405@ebsciences.com> Hello: This reply is from a lab microwave vendor; since you're now officially forewarned, no flames, please! :-) That said, for me, the usual suspect is the paraffin step. From the inception of microwave processing, this has always been the most problematic process, due to paraffin's non-polar nature. Pre-heating paraffin to the operating temperature of the protocol has always been extremely important; if the temperature of paraffin is raised by more than a degree or two when tissue is present, there is a strong potential for artifacts, inconsistency, and other problems. Here's why there's a critical difference between merely maintaining paraffin temperature, and raising paraffin temperature in the microwave. Paraffin does not absorb significant microwave energy; microwaves pass through wax almost as if it weren't there. So the energy has to be absorbed by "something," hence EBS' recommendation for a Pyrex processing container; Pyrex does absorb small amounts of microwave energy, transferring it to paraffin, maintaining a given temperature. Things get a little more delicate when using, for example, disposable plastic containers instead of Pyrex, making precise pre-heating even more critical. Pre-heating produces good, consistent results, since maintaining temperature does not expose patient samples to undue energy, but RAISING the temperature is another matter, requiring long wait times and, significantly, LOTS of excess energy. Where does this excess energy go? All too often, patient samples, which, even after fixation, dehydration, and clearing, are probably the most polar materials present. In effect, tissue can take the entire "hit." Since most melted paraffin in today's A.P. lab is somewhere around 60'0C, if lab personnel skip the pre-heating step, they are using the microwave to heat paraffin 14 - 24'0C. Besides taking a VERY long time, this can expose patient samples to WAY too much energy. There's also an unfortunate temptation to raise power output of the microwave since it's "taking too long," which may well be the worst thing to do. I know -- since we all use microwaves outside the lab, this whole concept is very counter-intuitive, which is why all EBS' microwaves have sported door warning labels exhorting users to pre-heat paraffin. Preheating paraffin to the operating temperature does require additional time, and equipment such as an oven or additional paraffin pot, assuming availability of a unit that can be set hot enough, plus sufficient space and electrical power. On the other hand, early attempts at microwave heating of paraffin resulted in questionable solutions like the addition of marble chips, glass marbles, glass discs and other more exotic (and expensive) materials. SHAMELESS COMMERCE WARNING: At EBS, we recognized the many benefits of the ability to microwave paraffin like any polar reagent: time savings, convenience, and standardization of procedures. So we developed PolarHeat(TM) (patent pending) disposable paraffin heating sheets. When exposed to microwave energy, a PolarHeat sheet, properly placed and immersed in paraffin, provides safe paraffin heating at a speed comparable to water or alcohol. Although significant tine savings are realized, more importantly, the sheets afford protection to irreplaceable tissue samples, "mission critical" in the context of patient care. Versions are available to fit popular EBS containers and racks; free sample packs are available. See [1]http://www.ebsstore.com/control/product/~category_id=C/~product_id= H2830 for more information, or feel free to contact me directly. Best regards, Phil McArdle -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Mobile: 860.597.6796 Fax: 860.653.0422 [2]pmcardle@ebsciences.com [3]www.ebsciences.com I skate to where the puck is going to be, not to where it's been. - Wayne Gretsky You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it, and it was probably my mistake anyway. So please do the right thing and make this e-mail go away. Thank you. References 1. http://www.ebsstore.com/control/product/~category_id=C/~product_id=H2830 2. mailto:pmcardle@ebsciences.com 3. http://www.ebsciences.com/ From gcallis <@t> montana.edu Mon May 21 10:29:19 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon May 21 10:29:20 2007 Subject: istotechnician artists has a history Re: [Histonet] Another question! In-Reply-To: <332113.64123.qm@web61219.mail.yahoo.com> References: <332113.64123.qm@web61219.mail.yahoo.com> Message-ID: <6.0.0.22.1.20070521091458.01b684d8@gemini.msu.montana.edu> Colleagues, On an aside, the "artist'" aspect to histotechnology has a long historical background. This is particularly apparent when you take Dr. McCormick's antique slide preparation workshop. The art was in the preparation of microscope slides made on glass scored and separated to obtain that slide. After the specimen was mounted under the coverglass the slide was carefully wrapped with beautiful paper to protect the viewer from cutting themselves on sharp edges. This produced an a slide reminiscent of old time framed, matted photographs, and the final labeling was done in the most gorgeous hand writing. Dr. McCormick also teaches some other ways of making slides in the most artistic fashion and the workshop is fun to take, and still being presented at NSH symposiums. I have some slides, one containing scales from a butterfly wing that remain iridescent even after preparation in the earlier 1900's. There is not doubt these slides were and are still valuable to the scientific communinity, artistically presented but highly viewable. At 12:11 PM 5/20/2007, you wrote: >Dear Colleagues: > By now you already know that from time to time I come up with a general > question, here is another one: > > Are we histotechs ARTISTS (creating new beautiful and original things > daily) or are we ARTISANS assuring that our work responds to certain > established norms in a most beautiful way? > > It would be nice to know how we, as a collective, feel about this > questions: artists or artisans? > > Ren? J. > > > > >--------------------------------- >Building a website is a piece of cake. >Yahoo! Small Business gives you all the tools to get online. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From gcallis <@t> montana.edu Mon May 21 10:35:36 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon May 21 10:35:33 2007 Subject: [Histonet] Re: Coplin pronunciation In-Reply-To: References: <001b01c79b2b$f8fa4a00$0201a8c0@puter> Message-ID: <6.0.0.22.1.20070521093213.01b660e8@gemini.msu.montana.edu> Have heard it both ways, but "cop-lynn" was how I learned it in esaly 60's. Jennifer brings up a good point as to where you learned the pronunciation. I have also heard them referred to as cuvets when in van der Loos lab in Amsterdam, no proper name involved. At 10:49 PM 5/20/2007, you wrote: >I was taught cope lynn. I went to school in Canada. Any difference? > > >Jennifer MacDonald Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 From Wanda.Smith <@t> HCAhealthcare.com Mon May 21 12:07:07 2007 From: Wanda.Smith <@t> HCAhealthcare.com (Smith Wanda) Date: Mon May 21 12:07:14 2007 Subject: [Histonet] Coplin In-Reply-To: <000101c799a7$254ba0d0$0202a8c0@HPPav2> References: <000101c799a7$254ba0d0$0202a8c0@HPPav2> Message-ID: <817C2761C5A1394180709AEEDB775B7E027AEB03@NASEV03.hca.corpad.net> My Momma's maiden name was Cope...so I say Cope lynn!!! Wanda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Friday, May 18, 2007 7:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Coplin Two nonsensical questions for late Friday night, concerning coplin jars. We got into a discussion about it this week, but I forgot to get onto Histonet Friday morning in time for the Friday trivia (though the CSI thread was good). 1. How do most people say "coplin"? - Cope lynn - Cop lynn We have people pronouncing it differently. Training difference? Difference in dialect? You say toe-may-toe, I say toe-mah-toe? Which brought us to question #2: 2. Who (maybe what) is Coplin/coplin? What nationality? That might make a difference in how the name should be pronounced. I've been looking for the origin/inventor/whatever, and I can't find anything. Thanks in advance. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Mon May 21 12:53:41 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Mon May 21 12:54:44 2007 Subject: [Histonet] Lysosomal / late endosomal marker In-Reply-To: References: Message-ID: <4651DCA5.2030705@umdnj.edu> Hi Seema; The classic method for lysosomes is a histochemical reaction for acid phosphatase. See any good histochemistry book for details. Geoff Seema Shroff wrote: >Hey, > > > >What is a good marker to label lysosomes / late endosomes in Central Nervous >System TISSUE SECTIONS. I'm trying to label this compartment in >oligodendrocytes in spinal cord cross sections - frozen or vibratome. > > > > > >Seema. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From Rrlopez2 <@t> aol.com Mon May 21 13:26:28 2007 From: Rrlopez2 <@t> aol.com (Rrlopez2@aol.com) Date: Mon May 21 13:26:36 2007 Subject: [Histonet] procedure for cleaning glassware Message-ID: Does anyone have a good procedure for cleaning glassware, one that CAP will approve of. Thanks in advance ************************************** See what's free at http://www.aol.com. From CBark <@t> memorialcare.org Mon May 21 13:46:31 2007 From: CBark <@t> memorialcare.org (Christine Bark) Date: Mon May 21 13:46:50 2007 Subject: [Histonet] Laboratory fume hood Message-ID: Happy Monday to all, I was hoping to get some help in finding a laboratory fume hood that satisfies California regulations. Starting in 2008, laboratory fume hoods need to have a quantitative airflow monitor and alarm (audible or visual). Is there anything I can purchase to attach to my current hoods or do I need to purchase new ones? Any information is welcome including vendors. Thank you, Christine Bark Senior Histotech, Pathology Saddleback Memorial Medical Center 949-452-3548 cbark@memorialcare.org ______________________________________________________________________________ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Jason.Wiese <@t> va.gov Mon May 21 15:08:21 2007 From: Jason.Wiese <@t> va.gov (Wiese, Jason VHAROS) Date: Mon May 21 15:08:31 2007 Subject: [Histonet] re: Friday Fun Fume In-Reply-To: References: Message-ID: <70EEF3D43B3C164C94037D811B2BE193E129E2@VHAV20MSGA3.v20.med.va.gov> Thank you Donna! I appreciate the suggestion. I was a little irritated on Friday, and I misdirected it at Joe. I apologized directly for it, and your suggestion is a great help to me. Thanks Again! JW -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Djemge@aol.com Sent: Saturday, May 19, 2007 2:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] re: Friday Fun Fume JW the histonet list server gives you the option of receiving batch digests. By batching all the messages you only get a couple of e-mails a day that includes all the topics and discussions. This has worked better for me than receiving an in box full of every single post. Someone on the histonet should be able to tell you how to make this switch to a batch digest. Donna Emge, HT(ASCP) Northwestern University 303 E. Superior, Lurie 7-220 312-503-2036 _d-emge@northwestern.edu_ (mailto:d-emge@northwestern.edu) djemge@aol.com ************************************** See what's free at http://www.aol.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> aol.com Mon May 21 16:45:48 2007 From: rsrichmond <@t> aol.com (rsrichmond@aol.com) Date: Mon May 21 16:46:01 2007 Subject: [Histonet] Re: Coplin pronunciation In-Reply-To: <200705211301.9fe4651d057396@rly-mg02.mx.aol.com> References: <200705211301.9fe4651d057396@rly-mg02.mx.aol.com> Message-ID: <8C96A08878643DC-1958-C33@FWM-D32.sysops.aol.com> I've always heard coplin, to rime with poplin or Janis Joplin. I checked some references, including the venerable "The Microtomist's Vade-Mecum" and my daddy's 1920 Dorland medical dictionary, as well as Google, and found nothing about who Coplin was. So the thing is called a "cuvette" - variously spelled - in Germany and the Netherlands? That would be fairly close the the English use of that curious word - a square glass spectrophotometer cell with optically flat sides. Apparently the word originally meant a bowl, and gradually acquired the meaning of a bowl-like container with square sides. It went in a different direction in French, where it came to mean a toilet bowl. Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From rchiovetti <@t> yahoo.com Mon May 21 17:19:25 2007 From: rchiovetti <@t> yahoo.com (Robert Chiovetti) Date: Mon May 21 17:19:31 2007 Subject: [Histonet] Laboratory fume hood Message-ID: <152053.35704.qm@web58907.mail.re1.yahoo.com> Christine, The most popular airflow meters I see on fume hoods are made by Dwyer Instruments, and they're called Magnehelic meters. They are frequently used as original equipment on fume hoods. They come with different scales on them, and they cost about $60 - $70. You can buy a flat mount to put the meter on your fume hood for about $25. I think you can get either a digital display model or the old-fashioned style with a needle and a scale. Check them out at: http://www.dwyer-inst.com/htdocs/pressure/Series2000Price.cfm Also, it would be a good idea to check with your facilities folks or the HVAC guys to make sure this meter would do the trick for you. If the State of California requires an alarm, I'll bet that Dwyer Instruments has something to meet that requirement. Disclaimer: I have no financial interests in Dwyer Instruments or Magnehelic. I jsut see a lot of them out there. Cheers, Bob Robert (Bob) Chiovetti, Ph.D. Southwest Precision Instruments Arizona's Microscopy Resource 132 North Elster Drive Tucson, AZ 85710-3212 Tel./Fax 520-546-4986 www.swpinet.com Member, Arizona Small Business Association (www.asba.com) ----- Original Message ---- From: Christine Bark To: histonet@lists.utsouthwestern.edu Sent: Monday, May 21, 2007 11:46:31 AM Subject: [Histonet] Laboratory fume hood Happy Monday to all, I was hoping to get some help in finding a laboratory fume hood that satisfies California regulations. Starting in 2008, laboratory fume hoods need to have a quantitative airflow monitor and alarm (audible or visual). Is there anything I can purchase to attach to my current hoods or do I need to purchase new ones? Any information is welcome including vendors. Thank you, Christine Bark Senior Histotech, Pathology Saddleback Memorial Medical Center 949-452-3548 cbark@memorialcare.org ______________________________________________________________________________ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Need Mail bonding? Go to the Yahoo! Mail Q&A for great tips from Yahoo! Answers users. http://answers.yahoo.com/dir/?link=list&sid=396546091 From jnocito <@t> satx.rr.com Sat May 26 18:01:46 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Mon May 21 18:01:49 2007 Subject: [Histonet] Coplin References: Message-ID: <009901c79fe9$d6c0bba0$d49eae18@yourxhtr8hvc4p> I coped Lynn once and got in trouble. Now I just say "one of those things over there". JTT ----- Original Message ----- From: "Jennifer MacDonald" To: "Cynthia Stone" Cc: ; Sent: Sunday, May 20, 2007 11:49 PM Subject: Re: [Histonet] Coplin >I was taught cope lynn. I went to school in Canada. Any difference? > > > Jennifer MacDonald > Director, Histotechnician Training Program > Mt. San Antonio College > 1100 N. Grand Ave. > Walnut, CA 91789 > (909) 594-5611 ext. 4884 > jmacdonald@mtsac.edu > > > > "Cynthia Stone" > Sent by: histonet-bounces@lists.utsouthwestern.edu > 05/20/2007 03:12 PM > Please respond to > Cynthia Stone > > > To > > cc > > Subject > Re: [Histonet] Coplin > > > > > > > I say Cop lin, like Copley Square in Boston. > > > ----- Original Message ----- > From: "Weems, Joyce" > To: ; > Sent: Sunday, May 20, 2007 5:23 PM > Subject: RE: [Histonet] Coplin > > > I say....Cope land. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & > Peggy Wenk > Sent: Friday, May 18, 2007 7:49 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Coplin > > Two nonsensical questions for late Friday night, concerning coplin jars. > We got into a discussion about it this week, but I forgot to get onto > Histonet Friday morning in time for the Friday trivia (though the CSI > thread was good). > > 1. How do most people say "coplin"? > - Cope lynn > - Cop lynn > > We have people pronouncing it differently. Training difference? > Difference in dialect? You say toe-may-toe, I say toe-mah-toe? > > Which brought us to question #2: > > 2. Who (maybe what) is Coplin/coplin? What nationality? That might make > a difference in how the name should be pronounced. I've been looking for > the origin/inventor/whatever, and I can't find anything. > > Thanks in advance. > > Peggy A. Wenk, HTL(ASCP)SLS > William Beaumont Hospital > Royal Oak, MI 48073 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice ** The information contained in this message may be > > privileged and is confidential information intended for the use of the > addressee listed above. If you are neither the intended recipient nor the > employee or agent responsible for delivering this message to the intended > recipient, you are hereby notified that any disclosure, copying, > distribution or the taking of any action in reliance on the contents of > this > information is strictly prohibited. If you have received this > communication > in error, please notify us immediately by replying to the message and > deleting it from your computer. Thank you. Saint Joseph's Health System, > Inc. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Sat May 26 18:07:40 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Mon May 21 18:07:43 2007 Subject: [Histonet] Lab Procedures References: <755548.97442.qm@web61219.mail.yahoo.com> Message-ID: <00a301c79fea$a9a1b7e0$d49eae18@yourxhtr8hvc4p> Derek 1. I use the old NCLSS format 2. I have about 100 procedures which include Surg Path, Special Stains, Immunos, Safety and Admin 3. I create all our procedures when I think it's necessary. If my pathologists had their way, I would have over 200 4. My medical director reviews and signs off on the procedures once a year 5. I create (because I can) a training and orientation guide for new employees. Hope this helps JTT ----- Original Message ----- From: "Rene J Buesa" To: "Derek Johnson" ; Sent: Monday, May 21, 2007 8:17 AM Subject: Re: [Histonet] Lab Procedures > Derek: > Short answers to your questions: > 1- NCLSS originally dictated the formate; > 2- About 40 all in the SOP (Standard Operating Procedures); > 3-The Supervisor selects new procedures and if s/he knows how, could > "create" new ones, to be "blind tested" and approved by the lab director; > and > 4- The same as going to Carnegie Hall: "practice, practice, practice" > Ren? J. > > Derek Johnson wrote: > Hello All- > > I am a histotechnology student and have a few questions I would like to > ask > this great community about your lab procedures. I don't want to be too > intrusive, but any info you can give would be wonderful! > > Without further ado, here are my questions: > 1) Who decides the format and required components of your lab's > procedures, > an accrediting organization or internal laboratory management? > > 2) About how many histotechnology related procedures exist in your > laboratory? Where are they kept? > > 3) Who is responsible for creating new lab procedures? Who is responsible > for reviewing your histology lab procedures? How often is it required to > be > done? > > 4) What is the method you use to train new personnel in your lab? > > Thanks for all your input! > > -Derek > > _________________________________________________________________ > PC Magazine's 2007 editors' choice for best Web mail-award-winning Windows > Live Hotmail. > http://imagine-windowslive.com/hotmail/?locale=en-us&ocid=TXT_TAGHM_migration_HM_mini_pcmag_0507 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Give spam the boot. Take control with tough spam protection > in the all-new Yahoo! Mail Beta. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Mon May 21 22:01:26 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon May 21 22:01:33 2007 Subject: [Histonet] CSI Las Vegas In-Reply-To: <000001c79ba9$e93f8160$d00f7ca5@lurie.northwestern.edu> Message-ID: A histo sales rep was the person actually cutting on that microtome. Her arms were shown,but it was the TV personality that we saw. The rep had a difficult time convincing the people about doing things the right way. Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu "Bernice Frederick" Sent by: histonet-bounces@lists.utsouthwestern.edu 05/21/2007 06:14 AM To "'Akemi Allison-Tacha'" cc histonet Subject RE: [Histonet] CSI Las Vegas At least "Bones" has been a little more accurate. They actually cut on a microtome (the right way) but I did question the fact that they never mentioned wether the bone they were cutting was decalled or not. Did anyone see S.O.B.? They did do it right. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Friday, May 18, 2007 10:00 AM To: Jackie M O'Connor; histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Cc: broed@aol.com Subject: Re: [Histonet] CSI Las Vegas I didn't catch the show last night, but years ago, there was a show called Quincy...very similar to CSI. My best friend Ed Brock was the technical consultant for that show. He worked for Technicon at that time and made sure they were atleast close to being on track. Times change. Akemi Allison-Tacha --- Jackie M O'Connor wrote: > Anyone catch the histology scene in CSI last night? > The medical examiner > took a piece of skin from a victim, put it in a > mold, poured wax on it, > put it in a microtome, turned the wheel backwards to > get a section on a > slide, melted the wax off with a Bunsen burner, then > looked at the section > under the microscope - -from that he determined that > the victim was > electrocuted. I love TV science. > > Happy Friday! > > Jackie O' > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sonya.martin <@t> soton.ac.uk Tue May 22 05:01:32 2007 From: sonya.martin <@t> soton.ac.uk (Martin S.) Date: Tue May 22 05:02:13 2007 Subject: [Histonet] NK cells in mouse tissue and PLP fixation Message-ID: <71437982F5B13A4D9A5B2669BDB89EE4023E35AE@ISS-CL-EX-V1.soton.ac.uk> Has anyone done any staining for NK cells in mouse tissue. We have an antibody against NK1.1 (PK136) but it doesnt seem to work on fresh-frozen tissues. I have looked through the literature and some people seem to have got iot to work on fresh-frozens while others have used PLP fixation before embedding in Tissue Tek. Has anyone had any experience with this Ab? Also, if I fix the tissues with PLP before freezing then how should I treat them before outting them in TissueTek to freeze? Thanks Sonya From relia1 <@t> earthlink.net Tue May 22 05:40:26 2007 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue May 22 05:40:31 2007 Subject: [Histonet] RELIA's Histology Opportunity Update 5-22-07 From Pam Barker Message-ID: Hi Histonetters! Right Place, Right Time, Right Opportunity. Sometimes your next career move is just a matter of being in the right place at the right time and ready for the next opportunity. Odds are that if you are contemplating a job change for whatever reason ? better compensation, a more desirable location or more challenging work, you don?t have the time to do a job search. Let me help you with that. I have clients located throughout the country. All of the jobs that I represent are full time day shift positions with excellent compensation, benefits and relocation/sign-on bonuses. I will assist you with your resume, coordination of interviews and coaching throughout the process. Remember my services are FREE of charge to you. Here is the latest update of the positions I am most excited to represent. If you are interested in any of these positions or a position like anyone of these but in another area either way ? give me a call toll free at 866-607-3542 or shoot me an e-mail at relia1@earthlink.net and let?s discuss it. Whether you are looking for a new position today, tomorrow or 6 months from now, it is never too early to have me keep a watch out for that perfect job for you! HISTOLOGY MANAGEMENT 1. Histology Manager ? Central CA 2. Histology Supervisor ? Southern CA (not Los Angeles) 3. Histology/Cytology Supervisor ? PA 4. Histology Manager ? Central Florida HISTOTECHNICIANS/TECHNOLOGISTS 1. Histology Inside Sales/Support ? WI 2. Lead Histotechnologist ? MA 3. Histo Tech ? OH ? Cleveland 4. Histo Tech ? OH ? Cincinatti (3rd shift) 5. Entry Level Histotech/MOHS trainee ? Los Angeles 6. Histo Tech ? Southern CA 7. Histo Tech ? Northern IL 8. Histo Tech ? MN 9. Histo Tech ? SW FL 10. Histo Tech ? N. Central FL 11. Histo Tech ? OK 12. Histo Tech ? MD/DC 13. Histo Tech ? RI Remember if nothing sounds interesting on my current list you can pass it on to your friends and wait for the next one OR give me a call or shoot me an e-mail telling me what you are looking for in your next opportunity. Remember timing is everything. Thanks ? Pam 866-60-RELIA (866-607-3542) Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net From gu.lang <@t> gmx.at Tue May 22 09:04:01 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue May 22 09:04:11 2007 Subject: WG: [Histonet] Coplin Message-ID: <000301c79c7a$0cd4e310$6412a8c0@dielangs.at> I have found a Pathologist, who worked in Philadelphia and wrote literature on pathologic methods from 1893-1913: William Michael Late Coplin. Unfortunately there is no hint on the pronounciation, but a beautiful picture: He might be the man behind the "Coplin-jar". http://clendening.kumc.edu/dc/pc/Coplin.jpg Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 From Maxim_71 <@t> mail.ru Tue May 22 09:23:35 2007 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Tue May 22 09:29:12 2007 Subject: [Histonet] Another question! Message-ID: <801169710.20070522182335@mail.ru> Rene: We are ARTISANS, because our permament materials (the tissues) was created by nature. And we are use chemical and physical knowledge, chemicals and many other facilities for finding interesting structure, substantia and etc. We gain already earlier created things... Sincerely, Maxim Peshkov Russia Taganrog From sonya.martin <@t> soton.ac.uk Tue May 22 09:54:30 2007 From: sonya.martin <@t> soton.ac.uk (Martin S.) Date: Tue May 22 09:55:25 2007 Subject: [Histonet] NK cells in mouse tissue and PLP fixation References: <71437982F5B13A4D9A5B2669BDB89EE4023E35AE@ISS-CL-EX-V1.soton.ac.uk> <6.0.0.22.1.20070522084714.01b56c88@gemini.msu.montana.edu> Message-ID: <71437982F5B13A4D9A5B2669BDB89EE4023E35AF@ISS-CL-EX-V1.soton.ac.uk> I just snap frooze the fresh tissue in isopentane on dry ice - this has worked with all the other markers I've been looking at but I gather NK cells are more tricky. I want to try PLP fixation before I freeze so do I put it straight from PLP to 30% sucrose or do I wash/use increasing amounts of sucrose. Once I've left the tissue overnight in sucrose can I then freeze it in TissueTek on dry ice as normal? Thanks Sonya -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: 22 May 2007 15:49 To: Martin S. Subject: Re: [Histonet] NK cells in mouse tissue and PLP fixation How did you fix in the first place? You need to sucrose cryoprotect after PLP fixation, in 30% sucrose overnight BEFORE snap freezing the tissues. At 04:01 AM 5/22/2007, you wrote: >Has anyone done any staining for NK cells in mouse tissue. We have an >antibody against NK1.1 (PK136) but it doesnt seem to work on >fresh-frozen tissues. I have looked through the literature and some >people seem to have got iot to work on fresh-frozens while others have >used PLP fixation before embedding in Tissue Tek. > >Has anyone had any experience with this Ab? > >Also, if I fix the tissues with PLP before freezing then how should I >treat them before outting them in TissueTek to freeze? > >Thanks >Sonya > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From LRaff <@t> lab.uropartners.com Tue May 22 10:04:18 2007 From: LRaff <@t> lab.uropartners.com (Lester Raff) Date: Tue May 22 10:04:23 2007 Subject: [Histonet] Please ask your colleagues doing FISH Message-ID: <5DA1CA5D0B98A84985B545A24423B822052F24@UPLAB01.uplab.local> Hello Histonetters: There is no current list serve for FISH (one is being developed) so I am asking histonetters for help. Please pass this on to anyone in your labs doing FISH testing. We are performing UroVysion FISH testing for bladder cancer. Lately we are seeing a strange orange red glow on our slides under the fluorescent scope. It is most noticeable on triple filter and gold filter. Abbott has offered some suggestions, but we are still having problems. Any suggestions??? Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 From AGrobe2555 <@t> aol.com Tue May 22 10:53:44 2007 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Tue May 22 10:53:51 2007 Subject: [Histonet] Microtome calibration? Message-ID: Good morning, A question was raised as to "how do you know the sections are the appropriate thickness?". Does one trust the settings on the microtome (Leitz 1512)? Is it an experienced eye that knows when they are "right"? Or is there some instrument that will do this measurment? Is there some way to calibrate the microtome to ensure that the sections actually are Xum? Thanks, Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital ************************************** See what's free at http://www.aol.com. From Barry.R.Rittman <@t> uth.tmc.edu Tue May 22 10:58:00 2007 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Tue May 22 10:58:05 2007 Subject: [Histonet] Another question! References: <801169710.20070522182335@mail.ru> Message-ID: I believe that an artisan is a craftsman who uses tools in a particular craft. The end result is a product that is made according to certain specifications. The end product may or may not have artistic value to some individuals. An artist on the other hand aims for a product that in, some cases they alone will be the only ones to perceive the end product as art. In most cases it is an extension of the artists persona. It may perform a function but I think that the main aim is to communicate the artists intent. Some of the end products that artisans produce are without a doubt artistic as well as performing a specific function. Some histotechs are artists and some are artisans. If you produce a well stained section and do not see the art in the cells, tissues and architacture then you are an artisan. The aim is to be both. Barry ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Maxim Peshkov Sent: Tue 5/22/2007 9:23 AM To: Rene J Buesa Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Another question! Rene: We are ARTISANS, because our permament materials (the tissues) was created by nature. And we are use chemical and physical knowledge, chemicals and many other facilities for finding interesting structure, substantia and etc. We gain already earlier created things... Sincerely, Maxim Peshkov Russia Taganrog _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From fawn <@t> cs.cmu.edu Tue May 22 11:14:57 2007 From: fawn <@t> cs.cmu.edu (Fawn Jones) Date: Tue May 22 11:14:51 2007 Subject: [Histonet] Job Opening Message-ID: <46531701.5090004@cs.cmu.edu> There is a job opening for a histo tech at Carnegie Mellon University in Pittsburgh, Pa. This person will be doing mainly plastic embedded tissues. Anyone interested can send their resumes to Fawn.Jones@gmail.com. It is very good pay and benefits. Thanks Fawn From rjbuesa <@t> yahoo.com Tue May 22 11:22:20 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue May 22 11:22:26 2007 Subject: [Histonet] Microtome calibration? In-Reply-To: Message-ID: <228619.55495.qm@web61214.mail.yahoo.com> Many years ago there were some methods developed to determine the thickness of sections, but changes in temperature of the wax after each section (due to friction) and the fact that wax expands with heat make those calibrations rather difficult. The best way is to realize the presence of layers y a section. Usually very thin sections will show an abundance of sectioned nuclei, and seldom more than one layer of lymphocytes, whose average diameter if 5-7 ?m If you are getting sections with well defined single layers of either RBC of lymphocytes, you are sectioning thin, if there are several layers of RBC or lymphocytes, you are sectioning on the thick side. Additionally, the microtome you mentioned is quite good in delivering the thickness it is set to work. Ren? J. AGrobe2555@aol.com wrote: Good morning, A question was raised as to "how do you know the sections are the appropriate thickness?". Does one trust the settings on the microtome (Leitz 1512)? Is it an experienced eye that knows when they are "right"? Or is there some instrument that will do this measurment? Is there some way to calibrate the microtome to ensure that the sections actually are Xum? Thanks, Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital ************************************** See what's free at http://www.aol.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Shape Yahoo! in your own image. Join our Network Research Panel today!http://us.rd.yahoo.com/evt=48517/*http://surveylink.yahoo.com/gmrs/yahoo_panel_invite.asp?a=7 hot CTA = Join our Network Research Panel From GDawson <@t> dynacaremilwaukee.com Tue May 22 11:23:35 2007 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Tue May 22 11:23:44 2007 Subject: [Histonet] Microtome calibration? In-Reply-To: Message-ID: Albert, I usually "eyeball" the sections and it is usually very plain to see if a section is significantly thicker or thinner when viewed on the microscope. A pathologist will definitely be able to tell if the thickness is off and, if he/she is anything like my docs, it will be pointed out immediately...so I guess my instrument would be the Pathologist9000. Great piece of equipment since it won't accept calibration even if it needs it. I see yet another CAP regulation coming on now...how unfortunate. Glen Dawson Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of AGrobe2555@aol.com Sent: Tuesday, May 22, 2007 10:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome calibration? Good morning, A question was raised as to "how do you know the sections are the appropriate thickness?". Does one trust the settings on the microtome (Leitz 1512)? Is it an experienced eye that knows when they are "right"? Or is there some instrument that will do this measurment? Is there some way to calibrate the microtome to ensure that the sections actually are Xum? Thanks, Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital ************************************** See what's free at http://www.aol.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Melissa.Gonzalez <@t> cellgenesys.com Tue May 22 13:01:43 2007 From: Melissa.Gonzalez <@t> cellgenesys.com (Melissa Gonzalez) Date: Tue May 22 13:01:52 2007 Subject: [Histonet] RE: NK cell on mouse tissue In-Reply-To: <20070522171346.A0EB727F146@mail.cellgenesys.com> References: <20070522171346.A0EB727F146@mail.cellgenesys.com> Message-ID: Hi Sonya, Funny that you ask, I have just run across this problem myself. I also don't have much luck using (various) NK1.1 markers on frozens. (I had one that worked fairly well, and tried again last week after some time that no longer works, ordered fresh, and still nothing.) I have been using CD56 from US Biological for a couple years now with consistent results. I am not familiar with PLP fixation.. what does it stand for and what is the reference for using it for NK's in particular? Are you getting any staining on your positive controls? Thanks and good luck Melissa ------------------Original Message---------------------------------- Has anyone done any staining for NK cells in mouse tissue. We have an antibody against NK1.1 (PK136) but it doesnt seem to work on fresh-frozen tissues. I have looked through the literature and some people seem to have got iot to work on fresh-frozens while others have used PLP fixation before embedding in Tissue Tek. Has anyone had any experience with this Ab? Also, if I fix the tissues with PLP before freezing then how should I treat them before outting them in TissueTek to freeze? Thanks Sonya -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: 22 May 2007 15:49 To: Martin S. Subject: Re: [Histonet] NK cells in mouse tissue and PLP fixation How did you fix in the first place? You need to sucrose cryoprotect after PLP fixation, in 30% sucrose overnight BEFORE snap freezing the tissues. -----Original Message----- From: "Martin S." Subject: RE: [Histonet] NK cells in mouse tissue and PLP fixation To: "Gayle Callis" Cc: histonet@lists.utsouthwestern.edu I just snap frooze the fresh tissue in isopentane on dry ice - this has worked with all the other markers I've been looking at but I gather NK cells are more tricky. I want to try PLP fixation before I freeze so do I put it straight from PLP to 30% sucrose or do I wash/use increasing amounts of sucrose. Once I've left the tissue overnight in sucrose can I then freeze it in TissueTek on dry ice as normal? Thanks Sonya From saulsbery.1 <@t> osu.edu Tue May 22 13:10:26 2007 From: saulsbery.1 <@t> osu.edu (Anne Saulsbery) Date: Tue May 22 13:10:34 2007 Subject: [Histonet] Chlorine gas Message-ID: <000001c79c9c$79436680$559a6ba4@cvm.vet.ohiostate.edu> Has any one heard that mixing decal solution and formalin would cause the formation of chlorine gas? From thoward <@t> unm.edu Tue May 22 13:12:29 2007 From: thoward <@t> unm.edu (Tamara A Howard) Date: Tue May 22 13:12:35 2007 Subject: [Histonet] FISH question (orange glow) Message-ID: Your "strange orange glow" sounds like it could be a mounting media problem - have you recently changed bottles or did someone leave the bottle out on the bench (if you are using one that should be stored cold)? I've seen background glow when a bottle was stored improperly or was too old, especially with the PPD-based anti-fade reagents. *************************** Tamara Howard Cell Biology & Physiology UNM-HSC Albuquerque, NM *************************** From carl.hobbs <@t> kcl.ac.uk Tue May 22 13:29:34 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Tue May 22 13:30:17 2007 Subject: [Histonet] Re: NK cells in mouse tissue and PLP fixation Message-ID: <000801c79c9f$25feb0d0$4101a8c0@carlba65530bda> I've used mouse anti CD57 ( clone NK1) on frozen, ( prefixed or fresh, then fixed) and pwax sections of mouse and found that Ab reagent to be very good. Doesn't help you with yours, I'm afraid. Maybe it will work on pwax sections, after HIER? A couple of NK-1 pics here http://www.immunoportal.com/ using above Ab on mouse pwax sections. Re PLP: must admit, I have never yet used an Ab that has needed it, rather than std 4% Formalin. Then again, Ks of Abs I've never used. Best wishes carl From rcharles <@t> state.pa.us Tue May 22 13:29:23 2007 From: rcharles <@t> state.pa.us (Charles, Roger) Date: Tue May 22 13:30:21 2007 Subject: [Histonet] -20C acetone fixative Message-ID: <12E4E17FEF6EBE4BAE95BEB3CDCB570006ACBB18@enhbgpri04.backup> Does any know or remember why acetone, when used as a fixative, is always used "ice cold" or in our case at -20C? Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 From Barry.R.Rittman <@t> uth.tmc.edu Tue May 22 13:35:10 2007 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Tue May 22 13:35:17 2007 Subject: [Histonet] Microtome calibration? References: Message-ID: The microtome setting is the indication of how far the mechanism will advance with each stroke. It cannot be an accurate measure of how thick a section will be cut as this depends on many other factors. In general the thinner the section setting, the greater the possible variation. One method that works is to stain a piece of fairly homogeneous tissue of known surface area that will accompany the specimen into the wax block. Once the section has been cut you can remove the calibration piece of tissue (proteins also works) and dissolve. Then measure the amount of dye using a spectrophotometer. This will give you the total volume of the calibration tissue and as you already known the cross sectional dimensions you can calculate the thickness of the section. As an alternate can mount the section, carry out image analysis of the mounted stained calibration tissue for optical density using this calibration tissue. Barry From: histonet-bounces@lists.utsouthwestern.edu on behalf of AGrobe2555@aol.com Sent: Tue 5/22/2007 10:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome calibration? Good morning, A question was raised as to "how do you know the sections are the appropriate thickness?". Does one trust the settings on the microtome (Leitz 1512)? Is it an experienced eye that knows when they are "right"? Or is there some instrument that will do this measurment? Is there some way to calibrate the microtome to ensure that the sections actually are Xum? Thanks, Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital ************************************** See what's free at http://www.aol.com . _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Barry.R.Rittman <@t> uth.tmc.edu Tue May 22 13:44:39 2007 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Tue May 22 13:44:46 2007 Subject: [Histonet] Coplin jar Message-ID: According to Bracegirdle (A History of Microtechnique 1987). The Coplin jar was introduced in 1897 as a method to deal with more than one section at a time. His reference is "Editorial note. Laboratory Dish. Journal Royal Microscopical Society. 237. 1898" From gcallis <@t> montana.edu Tue May 22 13:47:13 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue May 22 13:47:11 2007 Subject: [Histonet] RE: NK cell on mouse tissue In-Reply-To: References: <20070522171346.A0EB727F146@mail.cellgenesys.com> Message-ID: <6.0.0.22.1.20070522124212.01b2d740@gemini.msu.montana.edu> This NK1.1 (P136) mouse on mouse is not recommended for use with immunohistochemistry according to BD Bioscience technical data sheet. I am curious who has published success though, same as you and mentioned by Sonya)? Maybe she can provide the references on this. PLP stands for periodate lysine paraformaldehyde (McKean et al). At 12:01 PM 5/22/2007, you wrote: >Hi Sonya, >Funny that you ask, I have just run across this problem myself. I also >don't have much luck using (various) NK1.1 markers on frozens. (I had >one that worked fairly well, and tried again last week after some time >that no longer works, ordered fresh, and still nothing.) >I have been using CD56 from US Biological for a couple years now with >consistent results. >I am not familiar with PLP fixation.. what does it stand for and what is >the reference for using it for NK's in particular? >Are you getting any staining on your positive controls? Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From b-frederick <@t> northwestern.edu Tue May 22 14:03:43 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Tue May 22 14:03:58 2007 Subject: [Histonet] Microtome calibration? In-Reply-To: Message-ID: <000201c79ca3$ede26cf0$d00f7ca5@lurie.northwestern.edu> We actually had ours "calibrated by the guys who did our PM. They can do it- just ask Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of AGrobe2555@aol.com Sent: Tuesday, May 22, 2007 9:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome calibration? Good morning, A question was raised as to "how do you know the sections are the appropriate thickness?". Does one trust the settings on the microtome (Leitz 1512)? Is it an experienced eye that knows when they are "right"? Or is there some instrument that will do this measurment? Is there some way to calibrate the microtome to ensure that the sections actually are Xum? Thanks, Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital ************************************** See what's free at http://www.aol.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Tue May 22 14:06:10 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Tue May 22 14:06:18 2007 Subject: [Histonet] Chlorine gas In-Reply-To: <000001c79c9c$79436680$559a6ba4@cvm.vet.ohiostate.edu> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273C89@LSRIEXCH1.lsmaster.lifespan.org> Anne, I assume you are referring to decal solution made with hydrochloric acid, since there is no other possible source of chlorine in any decal solution that I know of. The chlorine in such a solution is in the form of chloride (Cl-) ions, and there is no reaction between such ions and formaldehyde molecules, so no chlorine gas could be generated in such a solution. If this were the case then formol-saline would also generate chlorine since it also contains chloride ions and formaldehyde. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Anne Saulsbery > Sent: Tuesday, May 22, 2007 2:10 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Chlorine gas > > Has any one heard that mixing decal solution and formalin would cause > the formation of chlorine gas? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From b-frederick <@t> northwestern.edu Tue May 22 14:06:24 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Tue May 22 14:06:40 2007 Subject: [Histonet] Chlorine gas In-Reply-To: <000001c79c9c$79436680$559a6ba4@cvm.vet.ohiostate.edu> Message-ID: <000501c79ca4$4d857530$d00f7ca5@lurie.northwestern.edu> N0- but mixing bleach and HCL (1% at that) will. I accidentally did it in histo school many moons ago. Was changing a stainer and just actually had pored the HCl in the sink and was rinsing the hemo container with bleach and let in run over. OOOPS Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anne Saulsbery Sent: Tuesday, May 22, 2007 12:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Chlorine gas Has any one heard that mixing decal solution and formalin would cause the formation of chlorine gas? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Tue May 22 14:25:02 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Tue May 22 14:25:16 2007 Subject: [Histonet] -20C acetone fixative In-Reply-To: <12E4E17FEF6EBE4BAE95BEB3CDCB570006ACBB18@enhbgpri04.backup> Message-ID: <000001c79ca6$e7e2c090$d00f7ca5@lurie.northwestern.edu> Because, if I'm not mistaken it blows up very easily. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles, Roger Sent: Tuesday, May 22, 2007 12:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] -20C acetone fixative Does any know or remember why acetone, when used as a fixative, is always used "ice cold" or in our case at -20C? Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From godsgalnow <@t> aol.com Tue May 22 14:33:04 2007 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Tue May 22 14:33:23 2007 Subject: [Histonet] Please ask your colleagues doing FISH In-Reply-To: <5DA1CA5D0B98A84985B545A24423B822052F24@UPLAB01.uplab.local> References: <5DA1CA5D0B98A84985B545A24423B822052F24@UPLAB01.uplab.local> Message-ID: <8C96ABF26E21996-190C-CA3@FWM-D20.sysops.aol.com> Have you checked the pH of your solutions?? What about the temperature of the water baths?? Are your times accurate in the pretreatments and hybridization and posthyb?? What about collection of the specimen, are you certain they are properly trained and the specimen are arriving cold and in the proper fixative? Roxanne Soto HT(ASCP)QIHC -----Original Message----- From: Lester Raff To: histonet@lists.utsouthwestern.edu Sent: Tue, 22 May 2007 11:04 am Subject: [Histonet] Please ask your colleagues doing FISH Hello Histonetters: There is no current list serve for FISH (one is being developed) so I am sking histonetters for help. Please pass this on to anyone in your abs doing FISH testing. We are performing UroVysion FISH testing for bladder cancer. Lately we re seeing a strange orange red glow on our slides under the fluorescent cope. It is most noticeable on triple filter and gold filter. Abbott as offered some suggestions, but we are still having problems. Any uggestions??? Lester J. Raff, MD edical Director roPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 h: 708-486-0076 ax: 708-486-0080 _______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From candy.a.bales <@t> uth.tmc.edu Tue May 22 14:46:04 2007 From: candy.a.bales <@t> uth.tmc.edu (Bales, Candy A) Date: Tue May 22 14:46:10 2007 Subject: [Histonet] cracks in paraffin Message-ID: Greetings. I am writing on behalf of a friend who is in need of assistance. Since purchasing a new embedding center, she has noticed that her paraffin blocks are cracked when she removes them from the molds. She has checked her temperatures on the embedding machine. She has not changed paraffin brands/types. She uses paraplast plus. She has tried spraying the molds. Any suggestions would be appreciated. Thank you C. Bales, H.T. University of Texas Dental Branch Houston, TX From PMonfils <@t> Lifespan.org Tue May 22 15:14:44 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Tue May 22 15:14:52 2007 Subject: [Histonet] cracks in paraffin In-Reply-To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E273C8A@LSRIEXCH1.lsmaster.lifespan.org> This is due to the cold plate being too cold. I once had a loaner embedding center while my own embedding center was being repaired, and I had the same problem. The cold plate on that unit was -8 degrees C, while the cold plate on my own unit was 0 degrees C. I found that the blocks would separate from the molds in much less time then they would on my own unit. In fact, the blocks would come loose from the molds while the top of the paraffin block still felt fairly warm to the touch. If you left the blocks on the cold plate until the tops of the blocks felt cold, you would find cracks in the cutting surface of the block. I did find however that these cracks were often just on the surface, and that in facing off the blocks I would get in past the cracks. Even when some cracks remained, they did not seriously affect sectioning of the blocks. But the cracks could be avoided by removing the blocks from the cold plate after a short period of time. If I had to continue using that unit I would have looked into having the cold plate temperature reduced, but since I only had it a couple of weeks, I just worked around the problem. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Bales, Candy A > Sent: Tuesday, May 22, 2007 3:46 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] cracks in paraffin > > Greetings. I am writing on behalf of a friend who is in need of > assistance. > > Since purchasing a new embedding center, she has noticed that her > paraffin blocks are cracked when she removes them from the molds. She > has checked her temperatures on the embedding machine. She has not > changed paraffin brands/types. She uses paraplast plus. > > She has tried spraying the molds. > > Any suggestions would be appreciated. > > Thank you > > C. Bales, H.T. > University of Texas Dental Branch > Houston, TX > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From doug <@t> ppspath.com Tue May 22 16:33:04 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue May 22 15:34:43 2007 Subject: [Histonet] cracks in paraffin In-Reply-To: Message-ID: Is her cold plate larger than her previous cold plate? Does she think that it gets colder than her previous cold plate? Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bales, Candy A Sent: Tuesday, May 22, 2007 2:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cracks in paraffin Greetings. I am writing on behalf of a friend who is in need of assistance. Since purchasing a new embedding center, she has noticed that her paraffin blocks are cracked when she removes them from the molds. She has checked her temperatures on the embedding machine. She has not changed paraffin brands/types. She uses paraplast plus. She has tried spraying the molds. Any suggestions would be appreciated. Thank you C. Bales, H.T. University of Texas Dental Branch Houston, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcresor <@t> lcpath.com Tue May 22 15:35:10 2007 From: jcresor <@t> lcpath.com (Jennifer Cresor) Date: Tue May 22 15:35:18 2007 Subject: [Histonet] Microwaveable slide racks Message-ID: Hello all, Can anyone out there recommend a slide rack that can be microwaved and not melt or catch on fire? We need to have something available to be able to speed up the process at those rush times. Any information or other ideas would be very appreciated. Thank you, Jennifer Longview, Wa jencres@lcpath.com From hhawkins <@t> utmb.edu Tue May 22 16:49:58 2007 From: hhawkins <@t> utmb.edu (Hawkins, Hal K.) Date: Tue May 22 16:50:09 2007 Subject: [Histonet] Coplin In-Reply-To: <000301c79c7a$0cd4e310$6412a8c0@dielangs.at> Message-ID: My Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, copyright 1984, by James L. Bennington, M.D. (a great reference) has an entry that says: Coplin jar (kop'lin) [W.M.L. Coplin, U.S. physician, 1864-1928] a wide-mouthed container made of glass or other material that is used in histology laboratories for the storage or staining of tissue sections. I was amazed when I Googled W.M.L. Coplin that this link turned up -- it is actually the article in Science by Coplin that describes the dish in detail. http://www.jstor.org/view/00368075/ap990143/99a00050/0 Of course, no hint as to pronunciation. Also, it turns out he was the President of the US-Canadian section of the International Association of Medical Museums in 1920/21. Google is amazing! Hal Hawkins UTMB, the University of Texas Medical Branch, Galveston, Texas -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Tuesday, May 22, 2007 9:04 AM To: histonet@lists.utsouthwestern.edu Subject: WG: [Histonet] Coplin I have found a Pathologist, who worked in Philadelphia and wrote literature on pathologic methods from 1893-1913: William Michael Late Coplin. Unfortunately there is no hint on the pronounciation, but a beautiful picture: He might be the man behind the "Coplin-jar". http://clendening.kumc.edu/dc/pc/Coplin.jpg Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Tue May 22 16:57:14 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue May 22 16:57:11 2007 Subject: [Histonet] comments on Murine NK1.1(P136) Message-ID: <6.0.0.22.1.20070522152235.01b2f068@gemini.msu.montana.edu> I was intrigued with the inquiry for this antibody and successful immunostaining. A quick literature search turned up one publication, which must be the same one Sonya was reading(?). Andrews DM, et el. NK1.1+ cells and murine cytomegalovirus infections: What happens in situ?, J Immunology 166:1796-1802, 2001. This publication provided some excellent insight on what fixation worked best for this antibody. They found the mouse had to be in vivo perfused with periodate lysine paraformaldehyde (PLP, McLean and Nakane, 1974, J Histochem Cytochem) for the best results. Immersion fixation was a poor choice of ex vivo frozen section from fresh tissue, snap frozen tissue. They had a fixation chart and also a chart with results of fixation with staining on liver, spleen lung. Also, it seemed to be important what strains of mice were used for this study. They also quenched autofluorescence. They did not indicate they did sucrose cryoprotection, but that would certainly make cryotomy easier with these tissues. They did immunofluorescence staining with confocal laser scanning microscopy and had exceptional photographs of staining. The authors commented that in situ identification of NK cells with this particular antibody clone (NK1.1, P 136) had been elusive "to date" (of this publication). That is probably one reason BD Bioscience states in their technical data sheet is that this antibody does not work for immunohistochemical staining. This antibody seems to one of the "picky" ones for tissue section work. Good luck on getting this one to work. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From JMacDonald <@t> mtsac.edu Tue May 22 17:55:47 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue May 22 17:55:54 2007 Subject: [Histonet] Chlorine gas In-Reply-To: <000001c79c9c$79436680$559a6ba4@cvm.vet.ohiostate.edu> Message-ID: According to F. Carson's book, if hydrochloric acid is used after formaldehyde fixation there is a potential for the formation of bis -chloromethyl ether, a carcinogen. Jennifer Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu "Anne Saulsbery" Sent by: histonet-bounces@lists.utsouthwestern.edu 05/22/2007 11:10 AM To cc Subject [Histonet] Chlorine gas Has any one heard that mixing decal solution and formalin would cause the formation of chlorine gas? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histology.bc <@t> shaw.ca Tue May 22 18:29:30 2007 From: histology.bc <@t> shaw.ca (Paul Bradbury) Date: Tue May 22 18:34:18 2007 Subject: [Histonet] Chlorine gas In-Reply-To: References: Message-ID: <46537CDA.3050607@shaw.ca> This topic has been discussed before on the Histonet. Do an Archive search for John Kiernan's comments on this subject. But, in a nutshell, don't get in a sweat over it ... it won't happen! Paul Bradbury Kamloops, BC, Canada Jennifer MacDonald wrote: > According to F. Carson's book, if hydrochloric acid is used after > formaldehyde fixation there is a potential for the formation of bis > -chloromethyl ether, a carcinogen. > > Jennifer > > > > > > Jennifer MacDonald > Director, Histotechnician Training Program > Mt. San Antonio College > 1100 N. Grand Ave. > Walnut, CA 91789 > (909) 594-5611 ext. 4884 > jmacdonald@mtsac.edu > > > > "Anne Saulsbery" > Sent by: histonet-bounces@lists.utsouthwestern.edu > 05/22/2007 11:10 AM > > To > > cc > > Subject > [Histonet] Chlorine gas > > > > > > > Has any one heard that mixing decal solution and formalin would cause > the formation of chlorine gas? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From jnocito <@t> satx.rr.com Sun May 27 18:36:31 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue May 22 18:36:28 2007 Subject: [Histonet] Chlorine gas References: <000001c79c9c$79436680$559a6ba4@cvm.vet.ohiostate.edu> Message-ID: <00ef01c7a0b7$db7e8630$d49eae18@yourxhtr8hvc4p> Wouldn't it change to formic acid? And y'all thought this was just a pretty face. JTT ----- Original Message ----- From: "Anne Saulsbery" To: Sent: Tuesday, May 22, 2007 1:10 PM Subject: [Histonet] Chlorine gas > Has any one heard that mixing decal solution and formalin would cause > the formation of chlorine gas? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brucea <@t> unimelb.edu.au Tue May 22 19:12:35 2007 From: brucea <@t> unimelb.edu.au (Bruce Abaloz) Date: Tue May 22 19:12:55 2007 Subject: [Histonet] NK cells in mouse tissue and PLP fixation Message-ID: I just snap frooze the fresh tissue in isopentane on dry ice - this has worked with all the other markers I've been looking at but I gather NK cells are more tricky. I want to try PLP fixation before I freeze so do I put it straight from PLP to 30% sucrose or do I wash/use increasing amounts of sucrose. Once I've left the tissue overnight in sucrose can I then freeze it in TissueTek on dry ice as normal? Thanks Sonya -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: 22 May 2007 15:49 To: Martin S. Subject: Re: [Histonet] NK cells in mouse tissue and PLP fixation How did you fix in the first place? You need to sucrose cryoprotect after PLP fixation, in 30% sucrose overnight BEFORE snap freezing the tissues. At 04:01 AM 5/22/2007, you wrote: >Has anyone done any staining for NK cells in mouse tissue. We have an >antibody against NK1.1 (PK136) but it doesnt seem to work on >fresh-frozen tissues. I have looked through the literature and some >people seem to have got iot to work on fresh-frozens while others have >used PLP fixation before embedding in Tissue Tek. > >Has anyone had any experience with this Ab? > >Also, if I fix the tissues with PLP before freezing then how should I >treat them before outting them in TissueTek to freeze? > >Thanks >Sonya > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- BRUCE ABALOZ HISTOLOGIST PH:61383446282 DEPT. of ZOOLOGY EMAIL: brucea@unimelb.edu.au THE UNIVERSITY of MELBOURNE. FAX:61383447909 VICTORIA. AUSTRALIA 3010 Nobody Can Make You Feel Inferior Without YOUR Permission - Eleanor Roosevelt WHETHER YOU THINK YOU CAN-OR WHETHER YOU THINK YOU CAN'T-YOU'RE RIGHT!! Be reasonable... Demand the impossible..... <')))>>< <')))>>< <')))>>< <')))>>< P Please consider the environment before printing this e-mail. From pmcardle <@t> ebsciences.com Wed May 23 07:44:38 2007 From: pmcardle <@t> ebsciences.com (Phil McArdle) Date: Wed May 23 07:44:51 2007 Subject: [Histonet] Microwaveable slide racks In-Reply-To: References: Message-ID: <46543736.6020006@ebsciences.com> Hello Jennifer: I'm from Energy Beam Sciences, manufacturer of lab microwaves. We carry microwaveable slide racks, including one that has yet to appear on our website because it's too new. Feel free to give me a call at 800-992-9037 extension 341. Best regards, Phil McArdle Jennifer Cresor wrote: > Hello all, > > Can anyone out there recommend a slide rack that can be microwaved and > not melt or catch on fire? We need to have something available to be > able to speed up the process at those rush times. Any information or > other ideas would be very appreciated. Thank you, > > Jennifer > Longview, Wa > jencres@lcpath.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Mobile: 860.597.6796 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com I skate to where the puck is going to be, not to where it's been. - Wayne Gretsky You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it, and it was probably my mistake anyway. So please do the right thing and make this e-mail go away. Thank you. From asmith <@t> mail.barry.edu Wed May 23 08:36:17 2007 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Wed May 23 08:36:50 2007 Subject: [Histonet] Chlorine gas In-Reply-To: <000001c79c9c$79436680$559a6ba4@cvm.vet.ohiostate.edu> Message-ID: <7DBCCC1FBC77C94F99F920D0CA6400B61E4095@exchsrv01.barrynet.barry.edu> It depends on what you use for decalcification. If you use Hollande's fluid, formic acid, or EDTA, there is no chloride to be oxidized to chlorine. My preference for decalcification is Schmorl's fluid (formalin-formic acid). In theory, formalin can react with hydrochloric acid to produce the dangerous volatile liquid bis-chloromethyl ether, but the yield from dilute solutions at atmospheric pressure is trivial. Nevertheless, if I mixed formalin and HCl I would do it in a fume hood. Mixing concentrated ammonium hydroxide and sodium hypochlorite ("chlorine bleach") produces a fairly good yield of chlorine: don't try it! Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anne Saulsbery Sent: Tuesday, May 22, 2007 2:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Chlorine gas Has any one heard that mixing decal solution and formalin would cause the formation of chlorine gas? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From ree3 <@t> leicester.ac.uk Wed May 23 08:43:38 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Wed May 23 08:43:54 2007 Subject: [Histonet] Chlorine gas In-Reply-To: <7DBCCC1FBC77C94F99F920D0CA6400B61E4095@exchsrv01.barrynet.barry.edu> References: <000001c79c9c$79436680$559a6ba4@cvm.vet.ohiostate.edu> <7DBCCC1FBC77C94F99F920D0CA6400B61E4095@exchsrv01.barrynet.barry.edu> Message-ID: Also known as Gooding and Stewarts(5-20% formic acid/ 5-20% formalin), depending if you are in a rush or not!, works a treat. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith, Allen Sent: 23 May 2007 14:36 To: Anne Saulsbery Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Chlorine gas It depends on what you use for decalcification. If you use Hollande's fluid, formic acid, or EDTA, there is no chloride to be oxidized to chlorine. My preference for decalcification is Schmorl's fluid (formalin-formic acid). In theory, formalin can react with hydrochloric acid to produce the dangerous volatile liquid bis-chloromethyl ether, but the yield from dilute solutions at atmospheric pressure is trivial. Nevertheless, if I mixed formalin and HCl I would do it in a fume hood. Mixing concentrated ammonium hydroxide and sodium hypochlorite ("chlorine bleach") produces a fairly good yield of chlorine: don't try it! Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anne Saulsbery Sent: Tuesday, May 22, 2007 2:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Chlorine gas Has any one heard that mixing decal solution and formalin would cause the formation of chlorine gas? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CBark <@t> memorialcare.org Wed May 23 09:12:06 2007 From: CBark <@t> memorialcare.org (Christine Bark) Date: Wed May 23 09:12:31 2007 Subject: [Histonet] Clarification on Laboratory Fume Hoods Message-ID: Good Morning, I received several emails asking about the new regulation that I referred to in my previous inquiry. This is, to my knowledge, just a California regulation and it becomes effective as of January 2008. Here is a link to the exact regulation: http://www.dir.ca.gov/Title8/5154_1.html Thank you in advance to all/any for your help. Christine Bark Senior Histotech, Pathology Saddleback Memorial Medical Center 949-452-3548 cbark@memorialcare.org ______________________________________________________________________________ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From JKOBLER <@t> PARTNERS.ORG Wed May 23 09:20:34 2007 From: JKOBLER <@t> PARTNERS.ORG (Kobler, James) Date: Wed May 23 09:20:43 2007 Subject: [Histonet] RE: Histonet Digest, Vol 42, Issue 32 In-Reply-To: <20070523141345.6D9CC4F8058@phsmgmx1.partners.org> References: <20070523141345.6D9CC4F8058@phsmgmx1.partners.org> Message-ID: <6D1DFC2837CEAE4BBBDED596071430854D07D4@PHSXMB4.partners.org> Dear histonet contributors, I would greatly appreciate any advice about antibodies that work well with ferret tissue (and any related processing tips). We are particularly interested in antibodies to markers for fibroblasts, myofibroblasts, endothelial, smooth muscle and inflammatory cells, as well as extracellular matrix. Thanks very much, Jim Kobler, Massachusetts General Hospital The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From lcastillos <@t> cogenics.com Wed May 23 09:28:19 2007 From: lcastillos <@t> cogenics.com (Castillos, Luminita) Date: Wed May 23 09:28:25 2007 Subject: [Histonet] Ethanol ready-to-use for LCM Message-ID: Hi, Does any know from where to buy ready-to-use ethanol (100%, 95%, 70%) molecular biology grade and RNase-free, compatible for Laser Capture microdissection staining protocol?. Thank you very much. L, From gcallis <@t> montana.edu Wed May 23 09:43:03 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed May 23 09:42:58 2007 Subject: [Histonet] NK cells in mouse tissue and PLP fixation In-Reply-To: References: Message-ID: <6.0.0.22.1.20070523083057.01b3a148@gemini.msu.montana.edu> Sonya, You can try PLP immersion fixation followed by sucrose cryoprotection, but the publication I read (reference was in a Histonet message yesterday) did a fixation study with PLP and other fixatives. They had little success with the NK1.1 (P136) antibody following immersion fixation with all fixatives, before snap freezing or after fresh tissue frozen sections were cut. They had nice staining following PERFUSION (with PLP) of the mouse. After perfusion, and once the tissues are dissected out, then immersion into this fixative should ensure complete fixation. You may want to do final fixation in PLP overnight, some do it for 5 - 6 hours longer here, then do the 30% sucrose cryoprotection at 4C. Just remove the tissue from fixative and go to the sucrose solution as Bruce suggested. Small fixed tissues often float on top of the sucrose solution. A general rule of thumb the that cryoprotection is done, tissues sink to the bottom of the container. Some people use a sucrose gradient, often seen in the literature, but we have never found that necessary. We blot off the excess sucrose solution before embedding in OCT. Hopefully you have access to a perfusion methods IF your immersion fails. Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 At 06:12 PM 5/22/2007, you wrote: >I just snap frooze the fresh tissue in isopentane on dry ice - this has >worked with all the other markers I've been looking at but I gather NK >cells are more tricky. >I want to try PLP fixation before I freeze so do I put it straight from >PLP to 30% sucrose or do I wash/use increasing amounts of sucrose. >Once I've left the tissue overnight in sucrose can I then freeze it in >TissueTek on dry ice as normal? > >Thanks >Sonya > >-----Original Message----- >From: Gayle Callis [mailto:gcallis@montana.edu] >Sent: 22 May 2007 15:49 >To: Martin S. >Subject: Re: [Histonet] NK cells in mouse tissue and PLP fixation > >How did you fix in the first place? > >You need to sucrose cryoprotect after PLP fixation, in 30% sucrose >overnight BEFORE snap freezing the tissues. > >At 04:01 AM 5/22/2007, you wrote: >>Has anyone done any staining for NK cells in mouse tissue. We have an >>antibody against NK1.1 (PK136) but it doesnt seem to work on >>fresh-frozen tissues. I have looked through the literature and some >>people seem to have got iot to work on fresh-frozens while others have >>used PLP fixation before embedding in Tissue Tek. >> >>Has anyone had any experience with this Ab? >> >>Also, if I fix the tissues with PLP before freezing then how should I >>treat them before outting them in TissueTek to freeze? >> >>Thanks >>Sonya >> >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >Gayle Callis >MT,HT,HTL(ASCP) >Research Histopathology Supervisor >Veterinary Molecular Biology >Montana State University - Bozeman >PO Box 173610 >Bozeman MT 59717-3610 > > > > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >-- >BRUCE ABALOZ >HISTOLOGIST PH:61383446282 >DEPT. of ZOOLOGY EMAIL: brucea@unimelb.edu.au >THE UNIVERSITY of MELBOURNE. FAX:61383447909 >VICTORIA. AUSTRALIA 3010 > Nobody Can Make You Feel Inferior Without YOUR Permission - > Eleanor Roosevelt > WHETHER YOU THINK YOU CAN-OR WHETHER YOU THINK YOU > CAN'T-YOU'RE RIGHT!! > Be reasonable... Demand the > impossible..... > <')))>>< <')))>>< > <')))>>< <')))>>< > P Please consider the environment before printing this e-mail. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.m.vanderloos <@t> amc.uva.nl Wed May 23 09:48:47 2007 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Wed May 23 09:50:46 2007 Subject: [Histonet] RE: -20C acetone fixative Message-ID: <37e62d383380.38338037e62d@amc.uva.nl> Long time ago they told me it is a milder fixative at -20C than at RT ??? Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [[1]mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles, Roger Sent: Tuesday, May 22, 2007 12:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] -20C acetone fixative Does any know or remember why acetone, when used as a fixative, is always used "ice cold" or in our case at -20C? Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 References 1. javascript:main.compose('new', 't=histonet-bounces@lists.utsouthwestern.edu') From ree3 <@t> leicester.ac.uk Wed May 23 10:15:14 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Wed May 23 10:15:19 2007 Subject: [Histonet] RE: -20C acetone fixative In-Reply-To: <37e62d383380.38338037e62d@amc.uva.nl> References: <37e62d383380.38338037e62d@amc.uva.nl> Message-ID: And me, in particular for enzyme histochemistry... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of C.M. van der Loos Sent: 23 May 2007 15:49 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: -20C acetone fixative Long time ago they told me it is a milder fixative at -20C than at RT ??? Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [[1]mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles, Roger Sent: Tuesday, May 22, 2007 12:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] -20C acetone fixative Does any know or remember why acetone, when used as a fixative, is always used "ice cold" or in our case at -20C? Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 References 1. javascript:main.compose('new', 't=histonet-bounces@lists.utsouthwestern.edu') _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward <@t> wfubmc.edu Wed May 23 10:02:18 2007 From: mward <@t> wfubmc.edu (Martha Ward) Date: Wed May 23 10:23:17 2007 Subject: [Histonet] Cryomold adapters Message-ID: <61135F0455D33347B5AAE209B903A3041B1C9EBD@EXCHVS2.medctr.ad.wfubmc.edu> I am looking for cryomold adapters and I hope someone can help. The ones we are using have a round center and are a white, hard plastic. I can find adapters that have a square center but that isn't what we want. I have tried the usual vendors but was hoping someone could help me. Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center Medical Center Blvd. Winston-Salem, NC 27157 336-716-2756 mward@wfubmc.edu From Robert.Lott <@t> TriadHospitals.com Wed May 23 10:23:14 2007 From: Robert.Lott <@t> TriadHospitals.com (Lott, Robert) Date: Wed May 23 10:23:24 2007 Subject: [Histonet] Cell Blocks on small buttons... Message-ID: <673832E27C45FC4D97EF758FFC777C27020E03DC@CPRTEVS01.triadhospitals.net> Hi Everyone, We are interested in hearing about different procedures for recovering small cytology aspirates (any fluid really!) ... and making a cell block from them using some type of coagulative process/fluid/etc. ... so that virtually all of the cells are retained! There may be some commercial products available ... would like to hear about those as well.!! Thanks!!!! Robert Robert L. Lott, HTL(ASCP) Manager, Anatomic Pathology LabFirst / Trinity Medical Center - formerly Montclair Baptist Medical Center 800 Montclair Road Birmingham, AL 35213 205-592-5388 phone 205-592-5646 fax robert.lott@triadhospitals.com From mlm11 <@t> cornell.edu Wed May 23 10:26:13 2007 From: mlm11 <@t> cornell.edu (Mary Lou Norman) Date: Wed May 23 10:26:18 2007 Subject: [Histonet] celloidin-ether/alc Message-ID: <6.2.1.2.2.20070523111029.04899930@postoffice9.mail.cornell.edu> Hello Histonet, Is there any other choice besides the cellodin-ether/alcohol coating? My parloidin is taking 'forever' to dissolve -- I have read a week but I could have used it a week ago. Thanks for your help. Mary Lou Norman From ploykasek <@t> phenopath.com Wed May 23 10:42:22 2007 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Wed May 23 10:46:41 2007 Subject: [Histonet] Work hours Message-ID: I was wondering if anyone in histoland has experienced moving from a schedule of standard 8 hour day/5 days a week to a 10 hour day/4 days a week with techs having a day off during the week. If so, what are the pros and cons of this type of schedule. I'm interested in feedback from both bench techs and supervisor/managers. We are in the initial stages of contemplating this and would appreciate info from anyone that has tried it. Thank you. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From Barry.R.Rittman <@t> uth.tmc.edu Wed May 23 11:37:45 2007 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Wed May 23 11:38:58 2007 Subject: [Histonet] celloidin-ether/alc References: <6.2.1.2.2.20070523111029.04899930@postoffice9.mail.cornell.edu> Message-ID: Mary Lou Will dissolve much more rapidly if soak the strips first in absolute ethanol for a day o so. The add the ether. Barry ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Mary Lou Norman Sent: Wed 5/23/2007 10:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] celloidin-ether/alc Hello Histonet, Is there any other choice besides the cellodin-ether/alcohol coating? My parloidin is taking 'forever' to dissolve -- I have read a week but I could have used it a week ago. Thanks for your help. Mary Lou Norman _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed May 23 11:52:11 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 23 11:54:35 2007 Subject: [Histonet] Work hours In-Reply-To: Message-ID: <990345.12651.qm@web61216.mail.yahoo.com> I have tried it. Cons: If yu don't have a flow of specimens in 4 days equivalent to the flow on 5 days, the personnel will be idling some time each day. If specimens or workload is available everybody will have work to do. Pros: it is fantastic having 3 days off weekly. Everybody likes that! Ren? J. Patti Loykasek wrote: I was wondering if anyone in histoland has experienced moving from a schedule of standard 8 hour day/5 days a week to a 10 hour day/4 days a week with techs having a day off during the week. If so, what are the pros and cons of this type of schedule. I'm interested in feedback from both bench techs and supervisor/managers. We are in the initial stages of contemplating this and would appreciate info from anyone that has tried it. Thank you. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. From mpence <@t> grhs.net Wed May 23 11:58:01 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Wed May 23 11:59:42 2007 Subject: [Histonet] Work hours In-Reply-To: <990345.12651.qm@web61216.mail.yahoo.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C5E3@IS-E2K3.grhs.net> How do you decide who gets which days off! Are they floating days? Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, May 23, 2007 11:52 AM To: Patti Loykasek; histonet Subject: Re: [Histonet] Work hours I have tried it. Cons: If yu don't have a flow of specimens in 4 days equivalent to the flow on 5 days, the personnel will be idling some time each day. If specimens or workload is available everybody will have work to do. Pros: it is fantastic having 3 days off weekly. Everybody likes that! Ren? J. Patti Loykasek wrote: I was wondering if anyone in histoland has experienced moving from a schedule of standard 8 hour day/5 days a week to a 10 hour day/4 days a week with techs having a day off during the week. If so, what are the pros and cons of this type of schedule. I'm interested in feedback from both bench techs and supervisor/managers. We are in the initial stages of contemplating this and would appreciate info from anyone that has tried it. Thank you. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Wed May 23 12:02:45 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed May 23 12:02:52 2007 Subject: [Histonet] Rinse times during immunohistochemical staining? Message-ID: <4EBFF65383B74D49995298C4976D1D5E273C8C@LSRIEXCH1.lsmaster.lifespan.org> What are the standard rinse times in buffer that people are using between steps of a standard immunohistochemical procedure? For example, after the primary antibody, before applying the secondary? How many rinses, and of what duration? From barrickstacey <@t> yahoo.com Wed May 23 12:19:02 2007 From: barrickstacey <@t> yahoo.com (Stacey Barrick) Date: Wed May 23 12:19:09 2007 Subject: [Histonet] Rinse times during immunohistochemical staining? In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E273C8C@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <290726.79563.qm@web54308.mail.yahoo.com> Usually I do 3 rinses- each for 15 mins "Monfils, Paul" wrote: What are the standard rinse times in buffer that people are using between steps of a standard immunohistochemical procedure? For example, after the primary antibody, before applying the secondary? How many rinses, and of what duration? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Boardwalk for $500? In 2007? Ha! Play Monopoly Here and Now (it's updated for today's economy) at Yahoo! Games. From dusko.trajkovic <@t> pfizer.com Wed May 23 12:59:34 2007 From: dusko.trajkovic <@t> pfizer.com (Trajkovic, Dusko) Date: Wed May 23 13:04:43 2007 Subject: [Histonet] Work hours In-Reply-To: <661949901A768E4F9CC16D8AF8F2838CA1C5E3@IS-E2K3.grhs.net> Message-ID: <3AD0BD3142459B4E9B12CBEAFF2B89B2047F4FF4@lajamrexm01.amer.pfizer.com> When we had the 10 hour days implemented, half of the crew would be off on Friday and the other half on Monday. We would switch off each month. During that switch weekend, one group of people would have a four day weekend and the other only a regular two day weekend. I might be biased on this subject, but I personally do not feel that there are any cons with this set up. I found that I could accomplish more in 4 days than in 8hr/5 day week. Dusko -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, May 23, 2007 9:58 AM To: Rene J Buesa; Patti Loykasek; histonet Subject: RE: [Histonet] Work hours How do you decide who gets which days off! Are they floating days? Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, May 23, 2007 11:52 AM To: Patti Loykasek; histonet Subject: Re: [Histonet] Work hours I have tried it. Cons: If yu don't have a flow of specimens in 4 days equivalent to the flow on 5 days, the personnel will be idling some time each day. If specimens or workload is available everybody will have work to do. Pros: it is fantastic having 3 days off weekly. Everybody likes that! Ren? J. Patti Loykasek wrote: I was wondering if anyone in histoland has experienced moving from a schedule of standard 8 hour day/5 days a week to a 10 hour day/4 days a week with techs having a day off during the week. If so, what are the pros and cons of this type of schedule. I'm interested in feedback from both bench techs and supervisor/managers. We are in the initial stages of contemplating this and would appreciate info from anyone that has tried it. Thank you. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- LEGAL NOTICE Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this E-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately. From gcallis <@t> montana.edu Wed May 23 13:16:35 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed May 23 13:16:31 2007 Subject: [Histonet] Rinse times during immunohistochemical staining? In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E273C8C@LSRIEXCH1.lsmaster. lifespan.org> References: <4EBFF65383B74D49995298C4976D1D5E273C8C@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <6.0.0.22.1.20070523113927.01b33158@gemini.msu.montana.edu> Paul, You pose an interesting question, and there seems to be a great deal of variation with times and steps, particularly when you read written protocols on the web or what people supply on Histonet. I presume you are talking about manual staining and not an automated stainer? For 5 um or less frozen or paraffin embedded sections, enzyme immunohistochemistry, I am not an excessive rinser, but have adequate rinses with excellent results. I found it did NOT make any difference in results if rinses were untimed or with more than one rinse. Timing rinses was abandoned a long time ago as, sorry for the pun as too "time consuming" . I reached a point of refusal with 3 to 5 minutes per rinse and with three changes - it simply did not jive with an busy IHC lifestyle for a day. Using a coplin jar for rinsing was a horror since we 20 or more slides at a time. For all immunostaining, we use manual Scytek humidity chambers that tip the slides into a slanted upright position, but slides stay in place. We do one untimed rinse between steps by flowing buffer from a wide tipped squirt bottle so buffer flows from above and across sections, slowly and gently - over and back one time. After rinse, buffer is either added to section until next step (have to open tubes, or some extra manual step) or I simply blot and add the next reagent. I based this rinse on Shandon coverplate method, where the well is filled, buffer flows down and over sections using a capillary gap, gravity flow. We found this takes approx. 2 -3 minutes to completely drain the well (one should time this), and NOT the 5 minutes they say to use. For immunofluorescence staining, I do the same thing, but tip buffer off for extra rinses after the fluorophore step. I tend to rinse with more steps for IFA staining, just to get rid of the fluorophore and any glowing junk. Since I do not have an automated immunostainer, what kind of rinsing step do these have? If these perform a single rinse without any particular time, then why not emulate the machine? I figure if a machine can do it, so can I. At 11:02 AM 5/23/2007, you wrote: >What are the standard rinse times in buffer that people are using between >steps of a standard immunohistochemical procedure? For example, after the >primary antibody, before applying the secondary? How many rinses, and of >what duration? Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From alaskagirl1950 <@t> yahoo.com Wed May 23 13:45:36 2007 From: alaskagirl1950 <@t> yahoo.com (Patricia Adams) Date: Wed May 23 13:45:40 2007 Subject: [Histonet] Work hours In-Reply-To: Message-ID: <193806.62416.qm@web52511.mail.re2.yahoo.com> When we did the 4, 10 hour days there were just two of us. Fridays were very busy, so 10 hours would turn into 12 for the one person working, and since we did not work weekends the Monday was very hard on the one person working. We also had it worked out so that we had a four day weekend one week and a two day the next. The only con was since there were only two people, the four day weekend was used to rest up. With more than two people on at a time there is no cons that I can see. We were pretty busy and did not have much down time at all, sometimes lucky to get lunch. We started the 10 hour days because we were so busy the late person got 10 to 12 hours easy, so that was a way to help the person get caught up with all the afternoon special stains,gross and slides to make. We also had a lot of afternoon frozens' and the Histoech cut and stained while the Pathologist went back to their office to wait "patiently" for the slides. And with one person cutting and staining mumultiple blocks and running back and forth to an office, it got hairy, Med-Techs knew that look of a HiHistoech with slides in hand and gave ground quickly. HmHm-mm....kind of miss the craziness of it all. Patricia Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D ____________________________________________________________________________________Choose the right car based on your needs. Check out Yahoo! Autos new Car Finder tool. http://autos.yahoo.com/carfinder/ From Karen.Heckford <@t> CHW.edu Wed May 23 14:00:44 2007 From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF) Date: Wed May 23 14:00:57 2007 Subject: [Histonet] Tissue Tek Cryostat Message-ID: Good Morning Everyone, We have a old Tissue Tek 11 4553 cryostat. We noticed after defrosting it that the temperature went way cold. We turned the dial which moves the black hand and set it for -25 and noticed that the blue hand is way past -30. We cannot find the users guide to save our lives. Probably lost a long time ago. Anyways can anyone tell me how to reset this thing. Cheers, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathology Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu From cfranssen <@t> nsalabs.com Wed May 23 14:22:53 2007 From: cfranssen <@t> nsalabs.com (Franssen, Dr. Catherine) Date: Wed May 23 14:23:57 2007 Subject: [Histonet] Work hours reply to patti loykosek In-Reply-To: References: Message-ID: <26C60098CBC18C4F97C2514517EDB43905228DDE@VS6.EXCHPROD.USA.NET> Patti- We're in the process of switching our histology staff to 5 8hr days from the 4 10hr days system. Originally it was implemented to account for long staining and slicing procedures that we specialize in, but improved technology makes that less of an issue and staggered times of arrival (8-4 or 9-5) take care of it. From the histologists' viewpoint the 4 10s were great because they could choose a Friday or Monday to take off and often they could take 4 day weekends, switching off weekends. (One or two part-timers cover any necessary weekend work, with full-timers available as needed.) It was great for morale and there was a nice coffee-and-doughnut kinship among the 7am crew (management/administration has always worked 5 8's... or 5 10s as it often turns into!). From the management perspective it got too difficult to work at half-staff for 2 days of the week. For a long time the part-timers filled in on those days, but as a small company, most of our histologists are senior and are very involved with project management... not having them in to supervise the part-time techs and communicate with clients pushed us over the edge. Plus, with increasing volumes, scheduling the work/specimen flow got too complex. Another note: on the 4-day system it is much more noticeable and hard to recover from someone taking a sick or personal day. In a larger company some of these issues may not exist... but there's my $0.04. Hello to HistoLand. Catherine Catherine Lowry Franssen, Ph.D. NeuroScience Associates 10915 Lake Ridge Drive Knoxville, TN 37934 tel. 865-675-2245 cel. 865-712-9314 fax. 865-675-2787 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, May 23, 2007 1:09 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 42, Issue 33 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Histonet Digest, Vol 42, Issue 32 (Kobler, James) 2. Ethanol ready-to-use for LCM (Castillos, Luminita) 3. RE: NK cells in mouse tissue and PLP fixation (Gayle Callis) 4. RE: -20C acetone fixative (C.M. van der Loos) 5. RE: RE: -20C acetone fixative (Edwards, R.E.) 6. Cryomold adapters (Martha Ward) 7. Cell Blocks on small buttons... (Lott, Robert) 8. celloidin-ether/alc (Mary Lou Norman) 9. Work hours (Patti Loykasek) 10. RE: celloidin-ether/alc (Rittman, Barry R) 11. Re: Work hours (Rene J Buesa) 12. RE: Work hours (Mike Pence) ---------------------------------------------------------------------- Message: 1 Date: Wed, 23 May 2007 10:20:34 -0400 From: "Kobler, James" Subject: [Histonet] RE: Histonet Digest, Vol 42, Issue 32 To: Message-ID: <6D1DFC2837CEAE4BBBDED596071430854D07D4@PHSXMB4.partners.org> Content-Type: text/plain; charset="us-ascii" Dear histonet contributors, I would greatly appreciate any advice about antibodies that work well with ferret tissue (and any related processing tips). We are particularly interested in antibodies to markers for fibroblasts, myofibroblasts, endothelial, smooth muscle and inflammatory cells, as well as extracellular matrix. Thanks very much, Jim Kobler, Massachusetts General Hospital The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. ------------------------------ Message: 2 Date: Wed, 23 May 2007 10:28:19 -0400 From: "Castillos, Luminita" Subject: [Histonet] Ethanol ready-to-use for LCM To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Hi, Does any know from where to buy ready-to-use ethanol (100%, 95%, 70%) molecular biology grade and RNase-free, compatible for Laser Capture microdissection staining protocol?. Thank you very much. L, ------------------------------ Message: 3 Date: Wed, 23 May 2007 08:43:03 -0600 From: Gayle Callis Subject: RE: [Histonet] NK cells in mouse tissue and PLP fixation To: Bruce Abaloz , Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20070523083057.01b3a148@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Sonya, You can try PLP immersion fixation followed by sucrose cryoprotection, but the publication I read (reference was in a Histonet message yesterday) did a fixation study with PLP and other fixatives. They had little success with the NK1.1 (P136) antibody following immersion fixation with all fixatives, before snap freezing or after fresh tissue frozen sections were cut. They had nice staining following PERFUSION (with PLP) of the mouse. After perfusion, and once the tissues are dissected out, then immersion into this fixative should ensure complete fixation. You may want to do final fixation in PLP overnight, some do it for 5 - 6 hours longer here, then do the 30% sucrose cryoprotection at 4C. Just remove the tissue from fixative and go to the sucrose solution as Bruce suggested. Small fixed tissues often float on top of the sucrose solution. A general rule of thumb the that cryoprotection is done, tissues sink to the bottom of the container. Some people use a sucrose gradient, often seen in the literature, but we have never found that necessary. We blot off the excess sucrose solution before embedding in OCT. Hopefully you have access to a perfusion methods IF your immersion fails. Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 At 06:12 PM 5/22/2007, you wrote: >I just snap frooze the fresh tissue in isopentane on dry ice - this has >worked with all the other markers I've been looking at but I gather NK >cells are more tricky. >I want to try PLP fixation before I freeze so do I put it straight from >PLP to 30% sucrose or do I wash/use increasing amounts of sucrose. >Once I've left the tissue overnight in sucrose can I then freeze it in >TissueTek on dry ice as normal? > >Thanks >Sonya > >-----Original Message----- >From: Gayle Callis [mailto:gcallis@montana.edu] >Sent: 22 May 2007 15:49 >To: Martin S. >Subject: Re: [Histonet] NK cells in mouse tissue and PLP fixation > >How did you fix in the first place? > >You need to sucrose cryoprotect after PLP fixation, in 30% sucrose >overnight BEFORE snap freezing the tissues. > >At 04:01 AM 5/22/2007, you wrote: >>Has anyone done any staining for NK cells in mouse tissue. We have an >>antibody against NK1.1 (PK136) but it doesnt seem to work on >>fresh-frozen tissues. I have looked through the literature and some >>people seem to have got iot to work on fresh-frozens while others have >>used PLP fixation before embedding in Tissue Tek. >> >>Has anyone had any experience with this Ab? >> >>Also, if I fix the tissues with PLP before freezing then how should I >>treat them before outting them in TissueTek to freeze? >> >>Thanks >>Sonya >> >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >Gayle Callis >MT,HT,HTL(ASCP) >Research Histopathology Supervisor >Veterinary Molecular Biology >Montana State University - Bozeman >PO Box 173610 >Bozeman MT 59717-3610 > > > > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >-- >BRUCE ABALOZ >HISTOLOGIST PH:61383446282 >DEPT. of ZOOLOGY EMAIL: brucea@unimelb.edu.au >THE UNIVERSITY of MELBOURNE. FAX:61383447909 >VICTORIA. AUSTRALIA 3010 > Nobody Can Make You Feel Inferior Without YOUR Permission - > Eleanor Roosevelt > WHETHER YOU THINK YOU CAN-OR WHETHER YOU THINK YOU > CAN'T-YOU'RE RIGHT!! > Be reasonable... Demand the > impossible..... > <')))>>< <')))>>< > <')))>>< <')))>>< > P Please consider the environment before printing this e-mail. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Wed, 23 May 2007 16:48:47 +0200 From: "C.M. van der Loos" Subject: [Histonet] RE: -20C acetone fixative To: histonet@lists.utsouthwestern.edu Message-ID: <37e62d383380.38338037e62d@amc.uva.nl> Content-Type: text/plain; charset="us-ascii" Long time ago they told me it is a milder fixative at -20C than at RT ??? Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [[1]mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles, Roger Sent: Tuesday, May 22, 2007 12:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] -20C acetone fixative Does any know or remember why acetone, when used as a fixative, is always used "ice cold" or in our case at -20C? Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 References 1. javascript:main.compose('new', 't=histonet-bounces@lists.utsouthwestern.edu') ------------------------------ Message: 5 Date: Wed, 23 May 2007 16:15:14 +0100 From: "Edwards, R.E." Subject: RE: [Histonet] RE: -20C acetone fixative To: "C.M. van der Loos" , Message-ID: Content-Type: text/plain; charset="US-ASCII" And me, in particular for enzyme histochemistry... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of C.M. van der Loos Sent: 23 May 2007 15:49 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: -20C acetone fixative Long time ago they told me it is a milder fixative at -20C than at RT ??? Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [[1]mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles, Roger Sent: Tuesday, May 22, 2007 12:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] -20C acetone fixative Does any know or remember why acetone, when used as a fixative, is always used "ice cold" or in our case at -20C? Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 References 1. javascript:main.compose('new', 't=histonet-bounces@lists.utsouthwestern.edu') _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Wed, 23 May 2007 11:02:18 -0400 From: "Martha Ward" Subject: [Histonet] Cryomold adapters To: Message-ID: <61135F0455D33347B5AAE209B903A3041B1C9EBD@EXCHVS2.medctr.ad.wfubmc.edu> Content-Type: text/plain; charset="us-ascii" I am looking for cryomold adapters and I hope someone can help. The ones we are using have a round center and are a white, hard plastic. I can find adapters that have a square center but that isn't what we want. I have tried the usual vendors but was hoping someone could help me. Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center Medical Center Blvd. Winston-Salem, NC 27157 336-716-2756 mward@wfubmc.edu ------------------------------ Message: 7 Date: Wed, 23 May 2007 10:23:14 -0500 From: "Lott, Robert" Subject: [Histonet] Cell Blocks on small buttons... To: Message-ID: <673832E27C45FC4D97EF758FFC777C27020E03DC@CPRTEVS01.triadhospitals.net> Content-Type: text/plain; charset="us-ascii" Hi Everyone, We are interested in hearing about different procedures for recovering small cytology aspirates (any fluid really!) ... and making a cell block from them using some type of coagulative process/fluid/etc. ... so that virtually all of the cells are retained! There may be some commercial products available ... would like to hear about those as well.!! Thanks!!!! Robert Robert L. Lott, HTL(ASCP) Manager, Anatomic Pathology LabFirst / Trinity Medical Center - formerly Montclair Baptist Medical Center 800 Montclair Road Birmingham, AL 35213 205-592-5388 phone 205-592-5646 fax robert.lott@triadhospitals.com ------------------------------ Message: 8 Date: Wed, 23 May 2007 11:26:13 -0400 From: Mary Lou Norman Subject: [Histonet] celloidin-ether/alc To: histonet@lists.utsouthwestern.edu Message-ID: <6.2.1.2.2.20070523111029.04899930@postoffice9.mail.cornell.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Hello Histonet, Is there any other choice besides the cellodin-ether/alcohol coating? My parloidin is taking 'forever' to dissolve -- I have read a week but I could have used it a week ago. Thanks for your help. Mary Lou Norman ------------------------------ Message: 9 Date: Wed, 23 May 2007 08:42:22 -0700 From: Patti Loykasek Subject: [Histonet] Work hours To: histonet Message-ID: Content-Type: text/plain; charset="US-ASCII" I was wondering if anyone in histoland has experienced moving from a schedule of standard 8 hour day/5 days a week to a 10 hour day/4 days a week with techs having a day off during the week. If so, what are the pros and cons of this type of schedule. I'm interested in feedback from both bench techs and supervisor/managers. We are in the initial stages of contemplating this and would appreciate info from anyone that has tried it. Thank you. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. ------------------------------ Message: 10 Date: Wed, 23 May 2007 11:37:45 -0500 From: "Rittman, Barry R" Subject: RE: [Histonet] celloidin-ether/alc To: "Mary Lou Norman" , Message-ID: Content-Type: text/plain; charset="iso-8859-1" Mary Lou Will dissolve much more rapidly if soak the strips first in absolute ethanol for a day o so. The add the ether. Barry ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Mary Lou Norman Sent: Wed 5/23/2007 10:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] celloidin-ether/alc Hello Histonet, Is there any other choice besides the cellodin-ether/alcohol coating? My parloidin is taking 'forever' to dissolve -- I have read a week but I could have used it a week ago. Thanks for your help. Mary Lou Norman _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Wed, 23 May 2007 09:52:11 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Work hours To: Patti Loykasek , histonet Message-ID: <990345.12651.qm@web61216.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I have tried it. Cons: If yu don't have a flow of specimens in 4 days equivalent to the flow on 5 days, the personnel will be idling some time each day. If specimens or workload is available everybody will have work to do. Pros: it is fantastic having 3 days off weekly. Everybody likes that! Ren? J. Patti Loykasek wrote: I was wondering if anyone in histoland has experienced moving from a schedule of standard 8 hour day/5 days a week to a 10 hour day/4 days a week with techs having a day off during the week. If so, what are the pros and cons of this type of schedule. I'm interested in feedback from both bench techs and supervisor/managers. We are in the initial stages of contemplating this and would appreciate info from anyone that has tried it. Thank you. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. ------------------------------ Message: 12 Date: Wed, 23 May 2007 11:58:01 -0500 From: "Mike Pence" Subject: RE: [Histonet] Work hours To: "Rene J Buesa" , "Patti Loykasek" , "histonet" Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C5E3@IS-E2K3.grhs.net> Content-Type: text/plain; charset="iso-8859-1" How do you decide who gets which days off! Are they floating days? Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, May 23, 2007 11:52 AM To: Patti Loykasek; histonet Subject: Re: [Histonet] Work hours I have tried it. Cons: If yu don't have a flow of specimens in 4 days equivalent to the flow on 5 days, the personnel will be idling some time each day. If specimens or workload is available everybody will have work to do. Pros: it is fantastic having 3 days off weekly. Everybody likes that! Ren? J. Patti Loykasek wrote: I was wondering if anyone in histoland has experienced moving from a schedule of standard 8 hour day/5 days a week to a 10 hour day/4 days a week with techs having a day off during the week. If so, what are the pros and cons of this type of schedule. I'm interested in feedback from both bench techs and supervisor/managers. We are in the initial stages of contemplating this and would appreciate info from anyone that has tried it. Thank you. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 42, Issue 33 **************************************** From rjbuesa <@t> yahoo.com Wed May 23 14:24:28 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 23 14:24:32 2007 Subject: [Histonet] Rinse times during immunohistochemical staining? In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E273C8C@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <205186.63619.qm@web61216.mail.yahoo.com> Paul: When I did IHC manually I used to lift the slide (after each incubation step) with one hand and flushed PBS over it squeezing a plastic bottle containing the buffer. The slides went then to a jar with PBS and stayed there until I flushed the last slide in the run, so they would stay there more or less time depending on how many slides I was running. Once the last slide was in the jar I started the next spet. The PBS in the jar was discarded after each group of slides was incubating. With automated stainers, that time is determined by the program and, again, the flooded slide will remain floded more time or less, depending on how many slides are in the run (at least that is how the DAKO autostainer works.) Hope this will help you. Renj J. "Monfils, Paul" wrote: What are the standard rinse times in buffer that people are using between steps of a standard immunohistochemical procedure? For example, after the primary antibody, before applying the secondary? How many rinses, and of what duration? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! oneSearch: Finally, mobile search that gives answers, not web links. From garret.t.miyamoto <@t> us.army.mil Wed May 23 14:32:45 2007 From: garret.t.miyamoto <@t> us.army.mil (garret.t.miyamoto@us.army.mil) Date: Wed May 23 14:33:01 2007 Subject: [Histonet] Re: Histonet Digest, Vol 42, Issue 33 In-Reply-To: <5s9rj3$kehs4@mxoutps1.us.army.mil> References: <5s9rj3$kehs4@mxoutps1.us.army.mil> Message-ID: ----- Original Message ----- From: histonet-request@lists.utsouthwestern.edu Date: Wednesday, May 23, 2007 7:13 am Subject: Histonet Digest, Vol 42, Issue 33 To: histonet@lists.utsouthwestern.edu > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: Histonet Digest, Vol 42, Issue 32 (Kobler, James) > 2. Ethanol ready-to-use for LCM (Castillos, Luminita) > 3. RE: NK cells in mouse tissue and PLP fixation (Gayle Callis) > 4. RE: -20C acetone fixative (C.M. van der Loos) > 5. RE: RE: -20C acetone fixative (Edwards, R.E.) > 6. Cryomold adapters (Martha Ward) > 7. Cell Blocks on small buttons... (Lott, Robert) > 8. celloidin-ether/alc (Mary Lou Norman) > 9. Work hours (Patti Loykasek) > 10. RE: celloidin-ether/alc (Rittman, Barry R) > 11. Re: Work hours (Rene J Buesa) > 12. RE: Work hours (Mike Pence) > > > ------------------------------------------------------------------- > --- > > Message: 1 > Date: Wed, 23 May 2007 10:20:34 -0400 > From: "Kobler, James" > Subject: [Histonet] RE: Histonet Digest, Vol 42, Issue 32 > To: > Message-ID: > <6D1DFC2837CEAE4BBBDED596071430854D07D4@PHSXMB4.partners.org> > Content-Type: text/plain; charset="us-ascii" > > Dear histonet contributors, > > I would greatly appreciate any advice about antibodies that work > well with > ferret tissue (and any related processing tips). We are > particularly interested > in antibodies to markers for fibroblasts, myofibroblasts, > endothelial, smooth > muscle and inflammatory cells, as well as extracellular matrix. > Thanks very > much, > > Jim Kobler, Massachusetts General Hospital > > > > > > The information transmitted in this electronic communication is > intended only for the person or entity to whom it is addressed and > may contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of or taking of any > action in reliance upon this information by persons or entities > other than the intended recipient is prohibited. If you received > this information in error, please contact the Compliance HelpLine > at 800-856-1983 and properly dispose of this information. > > > > > ------------------------------ > > Message: 2 > Date: Wed, 23 May 2007 10:28:19 -0400 > From: "Castillos, Luminita" > Subject: [Histonet] Ethanol ready-to-use for LCM > To: > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > > Hi, > > Does any know from where to buy ready-to-use ethanol (100%, 95%, 70%) > molecular biology grade and RNase-free, compatible for Laser Capture > microdissection staining protocol?. Thank you very much. > > L, > > > ------------------------------ > > Message: 3 > Date: Wed, 23 May 2007 08:43:03 -0600 > From: Gayle Callis > Subject: RE: [Histonet] NK cells in mouse tissue and PLP fixation > To: Bruce Abaloz , > Histonet@lists.utsouthwestern.edu > Message-ID: > <6.0.0.22.1.20070523083057.01b3a148@gemini.msu.montana.edu> > Content-Type: text/plain; charset="us-ascii"; format=flowed > > Sonya, > > You can try PLP immersion fixation followed by sucrose > cryoprotection, but > the publication I read (reference was in a Histonet message > yesterday) did > a fixation study with PLP and other fixatives. They had little > success > with the NK1.1 (P136) antibody following immersion fixation with > all > fixatives, before snap freezing or after fresh tissue frozen > sections were > cut. They had nice staining following PERFUSION (with PLP) of the > mouse. After perfusion, and once the tissues are dissected out, > then > immersion into this fixative should ensure complete fixation. You > may want > to do final fixation in PLP overnight, some do it for 5 - 6 hours > longer > here, then do the 30% sucrose cryoprotection at 4C. Just remove > the tissue > from fixative and go to the sucrose solution as Bruce suggested. > Small > fixed tissues often float on top of the sucrose solution. A > general rule > of thumb the that cryoprotection is done, tissues sink to the > bottom of the > container. > > Some people use a sucrose gradient, often seen in the literature, > but we > have never found that necessary. We blot off the excess sucrose > solution > before embedding in OCT. > > Hopefully you have access to a perfusion methods IF your immersion > fails. > Gayle Callis HTL, HT, MT(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University > Bozeman MT 59717 > > > At 06:12 PM 5/22/2007, you wrote: > >I just snap frooze the fresh tissue in isopentane on dry ice - > this has > >worked with all the other markers I've been looking at but I > gather NK > >cells are more tricky. > >I want to try PLP fixation before I freeze so do I put it > straight from > >PLP to 30% sucrose or do I wash/use increasing amounts of sucrose. > >Once I've left the tissue overnight in sucrose can I then freeze > it in > >TissueTek on dry ice as normal? > > > >Thanks > >Sonya > > > >-----Original Message----- > >From: Gayle Callis [mailto:gcallis@montana.edu] > >Sent: 22 May 2007 15:49 > >To: Martin S. > >Subject: Re: [Histonet] NK cells in mouse tissue and PLP fixation > > > >How did you fix in the first place? > > > >You need to sucrose cryoprotect after PLP fixation, in 30% sucrose > >overnight BEFORE snap freezing the tissues. > > > >At 04:01 AM 5/22/2007, you wrote: > >>Has anyone done any staining for NK cells in mouse tissue. We > have an > >>antibody against NK1.1 (PK136) but it doesnt seem to work on > >>fresh-frozen tissues. I have looked through the literature and some > >>people seem to have got iot to work on fresh-frozens while > others have > >>used PLP fixation before embedding in Tissue Tek. > >> > >>Has anyone had any experience with this Ab? > >> > >>Also, if I fix the tissues with PLP before freezing then how > should I > >>treat them before outting them in TissueTek to freeze? > >> > >>Thanks > >>Sonya > >> > >> > >> > >>_______________________________________________ > >>Histonet mailing list > >>Histonet@lists.utsouthwestern.edu > >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >Gayle Callis > >MT,HT,HTL(ASCP) > >Research Histopathology Supervisor > >Veterinary Molecular Biology > >Montana State University - Bozeman > >PO Box 173610 > >Bozeman MT 59717-3610 > > > > > > > > > > > > > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >-- > >BRUCE ABALOZ > >HISTOLOGIST PH:61383446282 > >DEPT. of ZOOLOGY EMAIL: brucea@unimelb.edu.au > >THE UNIVERSITY of MELBOURNE. FAX:61383447909 > >VICTORIA. AUSTRALIA 3010 > > Nobody Can Make You Feel Inferior Without YOUR > Permission - > > Eleanor Roosevelt > > WHETHER YOU THINK YOU CAN-OR WHETHER YOU > THINK YOU > > CAN'T-YOU'RE RIGHT!! > > Be reasonable... > Demand the > > impossible..... > > <')))>>< <')))>>< > > <')))>>< <')))>>< > > P Please consider the environment before printing > this e-mail. > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 4 > Date: Wed, 23 May 2007 16:48:47 +0200 > From: "C.M. van der Loos" > Subject: [Histonet] RE: -20C acetone fixative > To: histonet@lists.utsouthwestern.edu > Message-ID: <37e62d383380.38338037e62d@amc.uva.nl> > Content-Type: text/plain; charset="us-ascii" > > > Long time ago they told me it is a milder fixative at -20C > than at RT > ??? > > Chris van der Loos, PhD > Dept. of Pathology > Academic Medical Center M2-230 > Meibergdreef 9 > NL-1105 AZ Amsterdam > The Netherlands > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [[1]mailto:histonet-bounces@lists.utsouthwestern.edu] On > Behalf Of > Charles, > Roger > Sent: Tuesday, May 22, 2007 12:29 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] -20C acetone fixative > Does any know or remember why acetone, when used as a fixative, is > always used "ice cold" or in our case at -20C? > Roger Charles > Microbiologist > Pennsylvania Veterinary Laboratory > 2305 N Cameron St > Harrisburg, PA 17110 > 717-787-8808 > > References > > 1. javascript:main.compose('new', 't=histonet- > bounces@lists.utsouthwestern.edu') > > ------------------------------ > > Message: 5 > Date: Wed, 23 May 2007 16:15:14 +0100 > From: "Edwards, R.E." > Subject: RE: [Histonet] RE: -20C acetone fixative > To: "C.M. van der Loos" , > > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > And me, in particular for enzyme histochemistry... > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > C.M. van > der Loos > Sent: 23 May 2007 15:49 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: -20C acetone fixative > > > Long time ago they told me it is a milder fixative at -20C > than at > RT > ??? > > Chris van der Loos, PhD > Dept. of Pathology > Academic Medical Center M2-230 > Meibergdreef 9 > NL-1105 AZ Amsterdam > The Netherlands > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [[1]mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf > Of > Charles, > Roger > Sent: Tuesday, May 22, 2007 12:29 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] -20C acetone fixative > Does any know or remember why acetone, when used as a fixative, is > always used "ice cold" or in our case at -20C? > Roger Charles > Microbiologist > Pennsylvania Veterinary Laboratory > 2305 N Cameron St > Harrisburg, PA 17110 > 717-787-8808 > > References > > 1. javascript:main.compose('new', > 't=histonet-bounces@lists.utsouthwestern.edu') > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 6 > Date: Wed, 23 May 2007 11:02:18 -0400 > From: "Martha Ward" > Subject: [Histonet] Cryomold adapters > To: > Message-ID: > <61135F0455D33347B5AAE209B903A3041B1C9EBD@EXCHVS2.medctr.ad.wfubmc.edu> > > Content-Type: text/plain; charset="us-ascii" > > I am looking for cryomold adapters and I hope someone can help. The > ones we are using have a round center and are a white, hard > plastic. I > can find adapters that have a square center but that isn't what we > want.I have tried the usual vendors but was hoping someone could > help me. > Thanks in advance for your help. > > Martha Ward, MT (ASCP) QIHC > Assistant Manager, Molecular Diagnostics Lab > Wake Forest University Baptist Medical Center > Medical Center Blvd. > Winston-Salem, NC 27157 > 336-716-2756 > mward@wfubmc.edu > > > > ------------------------------ > > Message: 7 > Date: Wed, 23 May 2007 10:23:14 -0500 > From: "Lott, Robert" > Subject: [Histonet] Cell Blocks on small buttons... > To: > Message-ID: > <673832E27C45FC4D97EF758FFC777C27020E03DC@CPRTEVS01.triadhospitals.net> > > Content-Type: text/plain; charset="us-ascii" > > Hi Everyone, > > We are interested in hearing about different procedures for recovering > small cytology aspirates (any fluid really!) ... and making a cell > blockfrom them using some type of coagulative process/fluid/etc. > ... so that > virtually all of the cells are retained! > > > > There may be some commercial products available ... would like to hear > about those as well.!! > > > > Thanks!!!! > > > > Robert > > > > Robert L. Lott, HTL(ASCP) > > Manager, Anatomic Pathology > > LabFirst / Trinity Medical Center - formerly > > Montclair Baptist Medical Center > > 800 Montclair Road > > Birmingham, AL 35213 > > 205-592-5388 phone > > 205-592-5646 fax > > robert.lott@triadhospitals.com > > > > > > > ------------------------------ > > Message: 8 > Date: Wed, 23 May 2007 11:26:13 -0400 > From: Mary Lou Norman > Subject: [Histonet] celloidin-ether/alc > To: histonet@lists.utsouthwestern.edu > Message-ID: > <6.2.1.2.2.20070523111029.04899930@postoffice9.mail.cornell.edu> > Content-Type: text/plain; charset="us-ascii"; format=flowed > > Hello Histonet, > > Is there any other choice besides the cellodin-ether/alcohol > coating? My > parloidin is taking 'forever' to dissolve -- I have read a week > but I could > have used it a week ago. > > Thanks for your help. > Mary Lou Norman > > > > > ------------------------------ > > Message: 9 > Date: Wed, 23 May 2007 08:42:22 -0700 > From: Patti Loykasek > Subject: [Histonet] Work hours > To: histonet > Message-ID: > Content-Type: text/plain; charset="US-ASCII" > > I was wondering if anyone in histoland has experienced moving from a > schedule of standard 8 hour day/5 days a week to a 10 hour day/4 > days a > week with techs having a day off during the week. If so, what are > the pros > and cons of this type of schedule. I'm interested in feedback from > bothbench techs and supervisor/managers. We are in the initial > stages of > contemplating this and would appreciate info from anyone that has > tried it. > Thank you. > > > Patti Loykasek BS, HTL, QIHC > PhenoPath Laboratories > Seattle, WA > > > > ------------------------------------------------------------------- > ------ > This e-mail message, including any attachments, is for the sole > use of > the intended recipients and may contain privileged information. > Any > unauthorized review, use, disclosure or distribution is > prohibited. If > you are not the intended recipient, please contact the sender by e- > mail > and destroy all copies of the original message, or you may call > PhenoPath > Laboratories, Seattle, WA U.S.A. at (206) 374-9000. > > > > > ------------------------------ > > Message: 10 > Date: Wed, 23 May 2007 11:37:45 -0500 > From: "Rittman, Barry R" > Subject: RE: [Histonet] celloidin-ether/alc > To: "Mary Lou Norman" , > > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > Mary Lou > Will dissolve much more rapidly if soak the strips first in > absolute ethanol for a day o so. The add the ether. > Barry > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Mary > Lou Norman > Sent: Wed 5/23/2007 10:26 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] celloidin-ether/alc > > > > Hello Histonet, > > Is there any other choice besides the cellodin-ether/alcohol > coating? My > parloidin is taking 'forever' to dissolve -- I have read a week > but I could > have used it a week ago. > > Thanks for your help. > Mary Lou Norman > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 11 > Date: Wed, 23 May 2007 09:52:11 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Work hours > To: Patti Loykasek , histonet > > Message-ID: <990345.12651.qm@web61216.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > I have tried it. > Cons: If yu don't have a flow of specimens in 4 days equivalent > to the flow on 5 days, the personnel will be idling some time each > day. If specimens or workload is available everybody will have > work to do. > Pros: it is fantastic having 3 days off weekly. Everybody likes > that! Ren? J. > > Patti Loykasek wrote: > I was wondering if anyone in histoland has experienced moving > from a > schedule of standard 8 hour day/5 days a week to a 10 hour day/4 > days a > week with techs having a day off during the week. If so, what are > the pros > and cons of this type of schedule. I'm interested in feedback from > bothbench techs and supervisor/managers. We are in the initial > stages of > contemplating this and would appreciate info from anyone that has > tried it. > Thank you. > > > Patti Loykasek BS, HTL, QIHC > PhenoPath Laboratories > Seattle, WA > > > > ------------------------------------------------------------------- > ------ > This e-mail message, including any attachments, is for the sole > use of > the intended recipients and may contain privileged information. > Any > unauthorized review, use, disclosure or distribution is > prohibited. If > you are not the intended recipient, please contact the sender by e- > mail > and destroy all copies of the original message, or you may call > PhenoPath > Laboratories, Seattle, WA U.S.A. at (206) 374-9000. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Looking for a deal? Find great prices on flights and hotels with > Yahoo! FareChase. > > ------------------------------ > > Message: 12 > Date: Wed, 23 May 2007 11:58:01 -0500 > From: "Mike Pence" > Subject: RE: [Histonet] Work hours > To: "Rene J Buesa" , "Patti Loykasek" > , "histonet" > Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C5E3@IS-E2K3.grhs.net> > Content-Type: text/plain; charset="iso-8859-1" > > How do you decide who gets which days off! Are they floating days? > > Mike > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Wednesday, May 23, 2007 11:52 AM > To: Patti Loykasek; histonet > Subject: Re: [Histonet] Work hours > > > I have tried it. > Cons: If yu don't have a flow of specimens in 4 days equivalent > to the flow on 5 days, the personnel will be idling some time each > day. If specimens or workload is available everybody will have > work to do. > Pros: it is fantastic having 3 days off weekly. Everybody likes > that! Ren? J. > > Patti Loykasek wrote: > I was wondering if anyone in histoland has experienced moving > from a schedule of standard 8 hour day/5 days a week to a 10 hour > day/4 days a week with techs having a day off during the week. If > so, what are the pros and cons of this type of schedule. I'm > interested in feedback from both bench techs and > supervisor/managers. We are in the initial stages of contemplating > this and would appreciate info from anyone that has tried it. > Thank you. > > > Patti Loykasek BS, HTL, QIHC > PhenoPath Laboratories > Seattle, WA > > > > ------------------------------------------------------------------- > ------ > This e-mail message, including any attachments, is for the sole > use of the intended recipients and may contain privileged > information. Any > unauthorized review, use, disclosure or distribution is > prohibited. If > you are not the intended recipient, please contact the sender by e- > mail > and destroy all copies of the original message, or you may call > PhenoPath > Laboratories, Seattle, WA U.S.A. at (206) 374-9000. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > --------------------------------- > Looking for a deal? Find great prices on flights and hotels with > Yahoo! FareChase. _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 42, Issue 33 > **************************************** > From garret.t.miyamoto <@t> us.army.mil Wed May 23 14:44:27 2007 From: garret.t.miyamoto <@t> us.army.mil (garret.t.miyamoto@us.army.mil) Date: Wed May 23 14:44:35 2007 Subject: [Histonet] Re: Cell blocks on small buttons In-Reply-To: <5s9rj3$kehs4@mxoutps1.us.army.mil> References: <5s9rj3$kehs4@mxoutps1.us.army.mil> Message-ID: ----- Original Message ----- From: histonet-request@lists.utsouthwestern.edu Date: Wednesday, May 23, 2007 7:13 am Subject: Histonet Digest, Vol 42, Issue 33 To: histonet@lists.utsouthwestern.edu > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: Histonet Digest, Vol 42, Issue 32 (Kobler, James) > 2. Ethanol ready-to-use for LCM (Castillos, Luminita) > 3. RE: NK cells in mouse tissue and PLP fixation (Gayle Callis) > 4. RE: -20C acetone fixative (C.M. van der Loos) > 5. RE: RE: -20C acetone fixative (Edwards, R.E.) > 6. Cryomold adapters (Martha Ward) > 7. Cell Blocks on small buttons... (Lott, Robert) > 8. celloidin-ether/alc (Mary Lou Norman) > 9. Work hours (Patti Loykasek) > 10. RE: celloidin-ether/alc (Rittman, Barry R) > 11. Re: Work hours (Rene J Buesa) > 12. RE: Work hours (Mike Pence) > > > ------------------------------------------------------------------- > --- > > Message: 1 > Date: Wed, 23 May 2007 10:20:34 -0400 > From: "Kobler, James" > Subject: [Histonet] RE: Histonet Digest, Vol 42, Issue 32 > To: > Message-ID: > <6D1DFC2837CEAE4BBBDED596071430854D07D4@PHSXMB4.partners.org> > Content-Type: text/plain; charset="us-ascii" > > Dear histonet contributors, > > I would greatly appreciate any advice about antibodies that work > well with > ferret tissue (and any related processing tips). We are > particularly interested > in antibodies to markers for fibroblasts, myofibroblasts, > endothelial, smooth > muscle and inflammatory cells, as well as extracellular matrix. > Thanks very > much, > > Jim Kobler, Massachusetts General Hospital > > > > > > The information transmitted in this electronic communication is > intended only for the person or entity to whom it is addressed and > may contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of or taking of any > action in reliance upon this information by persons or entities > other than the intended recipient is prohibited. If you received > this information in error, please contact the Compliance HelpLine > at 800-856-1983 and properly dispose of this information. > > > > > ------------------------------ > > Message: 2 > Date: Wed, 23 May 2007 10:28:19 -0400 > From: "Castillos, Luminita" > Subject: [Histonet] Ethanol ready-to-use for LCM > To: > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > > Hi, > > Does any know from where to buy ready-to-use ethanol (100%, 95%, 70%) > molecular biology grade and RNase-free, compatible for Laser Capture > microdissection staining protocol?. Thank you very much. > > L, > > > ------------------------------ > > Message: 3 > Date: Wed, 23 May 2007 08:43:03 -0600 > From: Gayle Callis > Subject: RE: [Histonet] NK cells in mouse tissue and PLP fixation > To: Bruce Abaloz , > Histonet@lists.utsouthwestern.edu > Message-ID: > <6.0.0.22.1.20070523083057.01b3a148@gemini.msu.montana.edu> > Content-Type: text/plain; charset="us-ascii"; format=flowed > > Sonya, > > You can try PLP immersion fixation followed by sucrose > cryoprotection, but > the publication I read (reference was in a Histonet message > yesterday) did > a fixation study with PLP and other fixatives. They had little > success > with the NK1.1 (P136) antibody following immersion fixation with > all > fixatives, before snap freezing or after fresh tissue frozen > sections were > cut. They had nice staining following PERFUSION (with PLP) of the > mouse. After perfusion, and once the tissues are dissected out, > then > immersion into this fixative should ensure complete fixation. You > may want > to do final fixation in PLP overnight, some do it for 5 - 6 hours > longer > here, then do the 30% sucrose cryoprotection at 4C. Just remove > the tissue > from fixative and go to the sucrose solution as Bruce suggested. > Small > fixed tissues often float on top of the sucrose solution. A > general rule > of thumb the that cryoprotection is done, tissues sink to the > bottom of the > container. > > Some people use a sucrose gradient, often seen in the literature, > but we > have never found that necessary. We blot off the excess sucrose > solution > before embedding in OCT. > > Hopefully you have access to a perfusion methods IF your immersion > fails. > Gayle Callis HTL, HT, MT(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University > Bozeman MT 59717 > > > At 06:12 PM 5/22/2007, you wrote: > >I just snap frooze the fresh tissue in isopentane on dry ice - > this has > >worked with all the other markers I've been looking at but I > gather NK > >cells are more tricky. > >I want to try PLP fixation before I freeze so do I put it > straight from > >PLP to 30% sucrose or do I wash/use increasing amounts of sucrose. > >Once I've left the tissue overnight in sucrose can I then freeze > it in > >TissueTek on dry ice as normal? > > > >Thanks > >Sonya > > > >-----Original Message----- > >From: Gayle Callis [mailto:gcallis@montana.edu] > >Sent: 22 May 2007 15:49 > >To: Martin S. > >Subject: Re: [Histonet] NK cells in mouse tissue and PLP fixation > > > >How did you fix in the first place? > > > >You need to sucrose cryoprotect after PLP fixation, in 30% sucrose > >overnight BEFORE snap freezing the tissues. > > > >At 04:01 AM 5/22/2007, you wrote: > >>Has anyone done any staining for NK cells in mouse tissue. We > have an > >>antibody against NK1.1 (PK136) but it doesnt seem to work on > >>fresh-frozen tissues. I have looked through the literature and some > >>people seem to have got iot to work on fresh-frozens while > others have > >>used PLP fixation before embedding in Tissue Tek. > >> > >>Has anyone had any experience with this Ab? > >> > >>Also, if I fix the tissues with PLP before freezing then how > should I > >>treat them before outting them in TissueTek to freeze? > >> > >>Thanks > >>Sonya > >> > >> > >> > >>_______________________________________________ > >>Histonet mailing list > >>Histonet@lists.utsouthwestern.edu > >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >Gayle Callis > >MT,HT,HTL(ASCP) > >Research Histopathology Supervisor > >Veterinary Molecular Biology > >Montana State University - Bozeman > >PO Box 173610 > >Bozeman MT 59717-3610 > > > > > > > > > > > > > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >-- > >BRUCE ABALOZ > >HISTOLOGIST PH:61383446282 > >DEPT. of ZOOLOGY EMAIL: brucea@unimelb.edu.au > >THE UNIVERSITY of MELBOURNE. FAX:61383447909 > >VICTORIA. AUSTRALIA 3010 > > Nobody Can Make You Feel Inferior Without YOUR > Permission - > > Eleanor Roosevelt > > WHETHER YOU THINK YOU CAN-OR WHETHER YOU > THINK YOU > > CAN'T-YOU'RE RIGHT!! > > Be reasonable... > Demand the > > impossible..... > > <')))>>< <')))>>< > > <')))>>< <')))>>< > > P Please consider the environment before printing > this e-mail. > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 4 > Date: Wed, 23 May 2007 16:48:47 +0200 > From: "C.M. van der Loos" > Subject: [Histonet] RE: -20C acetone fixative > To: histonet@lists.utsouthwestern.edu > Message-ID: <37e62d383380.38338037e62d@amc.uva.nl> > Content-Type: text/plain; charset="us-ascii" > > > Long time ago they told me it is a milder fixative at -20C > than at RT > ??? > > Chris van der Loos, PhD > Dept. of Pathology > Academic Medical Center M2-230 > Meibergdreef 9 > NL-1105 AZ Amsterdam > The Netherlands > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [[1]mailto:histonet-bounces@lists.utsouthwestern.edu] On > Behalf Of > Charles, > Roger > Sent: Tuesday, May 22, 2007 12:29 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] -20C acetone fixative > Does any know or remember why acetone, when used as a fixative, is > always used "ice cold" or in our case at -20C? > Roger Charles > Microbiologist > Pennsylvania Veterinary Laboratory > 2305 N Cameron St > Harrisburg, PA 17110 > 717-787-8808 > > References > > 1. javascript:main.compose('new', 't=histonet- > bounces@lists.utsouthwestern.edu') > > ------------------------------ > > Message: 5 > Date: Wed, 23 May 2007 16:15:14 +0100 > From: "Edwards, R.E." > Subject: RE: [Histonet] RE: -20C acetone fixative > To: "C.M. van der Loos" , > > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > And me, in particular for enzyme histochemistry... > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > C.M. van > der Loos > Sent: 23 May 2007 15:49 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: -20C acetone fixative > > > Long time ago they told me it is a milder fixative at -20C > than at > RT > ??? > > Chris van der Loos, PhD > Dept. of Pathology > Academic Medical Center M2-230 > Meibergdreef 9 > NL-1105 AZ Amsterdam > The Netherlands > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [[1]mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf > Of > Charles, > Roger > Sent: Tuesday, May 22, 2007 12:29 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] -20C acetone fixative > Does any know or remember why acetone, when used as a fixative, is > always used "ice cold" or in our case at -20C? > Roger Charles > Microbiologist > Pennsylvania Veterinary Laboratory > 2305 N Cameron St > Harrisburg, PA 17110 > 717-787-8808 > > References > > 1. javascript:main.compose('new', > 't=histonet-bounces@lists.utsouthwestern.edu') > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 6 > Date: Wed, 23 May 2007 11:02:18 -0400 > From: "Martha Ward" > Subject: [Histonet] Cryomold adapters > To: > Message-ID: > <61135F0455D33347B5AAE209B903A3041B1C9EBD@EXCHVS2.medctr.ad.wfubmc.edu> > > Content-Type: text/plain; charset="us-ascii" > > I am looking for cryomold adapters and I hope someone can help. The > ones we are using have a round center and are a white, hard > plastic. I > can find adapters that have a square center but that isn't what we > want.I have tried the usual vendors but was hoping someone could > help me. > Thanks in advance for your help. > > Martha Ward, MT (ASCP) QIHC > Assistant Manager, Molecular Diagnostics Lab > Wake Forest University Baptist Medical Center > Medical Center Blvd. > Winston-Salem, NC 27157 > 336-716-2756 > mward@wfubmc.edu > > > > ------------------------------ > > Message: 7 > Date: Wed, 23 May 2007 10:23:14 -0500 > From: "Lott, Robert" > Subject: [Histonet] Cell Blocks on small buttons... > To: > Message-ID: > <673832E27C45FC4D97EF758FFC777C27020E03DC@CPRTEVS01.triadhospitals.net> > > Content-Type: text/plain; charset="us-ascii" > > Hi Everyone, > > We are interested in hearing about different procedures for recovering > small cytology aspirates (any fluid really!) ... and making a cell > blockfrom them using some type of coagulative process/fluid/etc. > ... so that > virtually all of the cells are retained! > > > > There may be some commercial products available ... would like to hear > about those as well.!! > > > > Thanks!!!! > > > > Robert > > > > Robert L. Lott, HTL(ASCP) > > Manager, Anatomic Pathology > > LabFirst / Trinity Medical Center - formerly > > Montclair Baptist Medical Center > > 800 Montclair Road > > Birmingham, AL 35213 > > 205-592-5388 phone > > 205-592-5646 fax > > robert.lott@triadhospitals.com > > > > > > > ------------------------------ > > Message: 8 > Date: Wed, 23 May 2007 11:26:13 -0400 > From: Mary Lou Norman > Subject: [Histonet] celloidin-ether/alc > To: histonet@lists.utsouthwestern.edu > Message-ID: > <6.2.1.2.2.20070523111029.04899930@postoffice9.mail.cornell.edu> > Content-Type: text/plain; charset="us-ascii"; format=flowed > > Hello Histonet, > > Is there any other choice besides the cellodin-ether/alcohol > coating? My > parloidin is taking 'forever' to dissolve -- I have read a week > but I could > have used it a week ago. > > Thanks for your help. > Mary Lou Norman > > > > > ------------------------------ > > Message: 9 > Date: Wed, 23 May 2007 08:42:22 -0700 > From: Patti Loykasek > Subject: [Histonet] Work hours > To: histonet > Message-ID: > Content-Type: text/plain; charset="US-ASCII" > > I was wondering if anyone in histoland has experienced moving from a > schedule of standard 8 hour day/5 days a week to a 10 hour day/4 > days a > week with techs having a day off during the week. If so, what are > the pros > and cons of this type of schedule. I'm interested in feedback from > bothbench techs and supervisor/managers. We are in the initial > stages of > contemplating this and would appreciate info from anyone that has > tried it. > Thank you. > > > Patti Loykasek BS, HTL, QIHC > PhenoPath Laboratories > Seattle, WA > > > > ------------------------------------------------------------------- > ------ > This e-mail message, including any attachments, is for the sole > use of > the intended recipients and may contain privileged information. > Any > unauthorized review, use, disclosure or distribution is > prohibited. If > you are not the intended recipient, please contact the sender by e- > mail > and destroy all copies of the original message, or you may call > PhenoPath > Laboratories, Seattle, WA U.S.A. at (206) 374-9000. > > > > > ------------------------------ > > Message: 10 > Date: Wed, 23 May 2007 11:37:45 -0500 > From: "Rittman, Barry R" > Subject: RE: [Histonet] celloidin-ether/alc > To: "Mary Lou Norman" , > > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > Mary Lou > Will dissolve much more rapidly if soak the strips first in > absolute ethanol for a day o so. The add the ether. > Barry > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Mary > Lou Norman > Sent: Wed 5/23/2007 10:26 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] celloidin-ether/alc > > > > Hello Histonet, > > Is there any other choice besides the cellodin-ether/alcohol > coating? My > parloidin is taking 'forever' to dissolve -- I have read a week > but I could > have used it a week ago. > > Thanks for your help. > Mary Lou Norman > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 11 > Date: Wed, 23 May 2007 09:52:11 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Work hours > To: Patti Loykasek , histonet > > Message-ID: <990345.12651.qm@web61216.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > I have tried it. > Cons: If yu don't have a flow of specimens in 4 days equivalent > to the flow on 5 days, the personnel will be idling some time each > day. If specimens or workload is available everybody will have > work to do. > Pros: it is fantastic having 3 days off weekly. Everybody likes > that! Ren? J. > > Patti Loykasek wrote: > I was wondering if anyone in histoland has experienced moving > from a > schedule of standard 8 hour day/5 days a week to a 10 hour day/4 > days a > week with techs having a day off during the week. If so, what are > the pros > and cons of this type of schedule. I'm interested in feedback from > bothbench techs and supervisor/managers. We are in the initial > stages of > contemplating this and would appreciate info from anyone that has > tried it. > Thank you. > > > Patti Loykasek BS, HTL, QIHC > PhenoPath Laboratories > Seattle, WA > > > > ------------------------------------------------------------------- > ------ > This e-mail message, including any attachments, is for the sole > use of > the intended recipients and may contain privileged information. > Any > unauthorized review, use, disclosure or distribution is > prohibited. If > you are not the intended recipient, please contact the sender by e- > mail > and destroy all copies of the original message, or you may call > PhenoPath > Laboratories, Seattle, WA U.S.A. at (206) 374-9000. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Looking for a deal? Find great prices on flights and hotels with > Yahoo! FareChase. > > ------------------------------ > > Message: 12 > Date: Wed, 23 May 2007 11:58:01 -0500 > From: "Mike Pence" > Subject: RE: [Histonet] Work hours > To: "Rene J Buesa" , "Patti Loykasek" > , "histonet" > Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C5E3@IS-E2K3.grhs.net> > Content-Type: text/plain; charset="iso-8859-1" > > How do you decide who gets which days off! Are they floating days? > > Mike > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Wednesday, May 23, 2007 11:52 AM > To: Patti Loykasek; histonet > Subject: Re: [Histonet] Work hours > > > I have tried it. > Cons: If yu don't have a flow of specimens in 4 days equivalent > to the flow on 5 days, the personnel will be idling some time each > day. If specimens or workload is available everybody will have > work to do. > Pros: it is fantastic having 3 days off weekly. Everybody likes > that! Ren? J. > > Patti Loykasek wrote: > I was wondering if anyone in histoland has experienced moving > from a schedule of standard 8 hour day/5 days a week to a 10 hour > day/4 days a week with techs having a day off during the week. If > so, what are the pros and cons of this type of schedule. I'm > interested in feedback from both bench techs and > supervisor/managers. We are in the initial stages of contemplating > this and would appreciate info from anyone that has tried it. > Thank you. > > > Patti Loykasek BS, HTL, QIHC > PhenoPath Laboratories > Seattle, WA > > > > ------------------------------------------------------------------- > ------ > This e-mail message, including any attachments, is for the sole > use of the intended recipients and may contain privileged > information. Any > unauthorized review, use, disclosure or distribution is > prohibited. If > you are not the intended recipient, please contact the sender by e- > mail > and destroy all copies of the original message, or you may call > PhenoPath > Laboratories, Seattle, WA U.S.A. at (206) 374-9000. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > --------------------------------- > Looking for a deal? Find great prices on flights and hotels with > Yahoo! FareChase. _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 42, Issue 33 > **************************************** > Robert, I asked someone in our cytology section about your inquiry. What they do is spin down the specinen in a centrifuge, decant the liquid, add about four drops of "HistoGel" (specimen processing gel from Richard-Allen Scientific, 12 vials - 10 ml per vial, Reorder number HG-4000-012), respin the specimen and collect the gel button, put it into a tissue specimen bag before placing it into the cassette. I hope this will work for you. Garret From wanpto <@t> aol.com Wed May 23 14:50:21 2007 From: wanpto <@t> aol.com (wanpto@aol.com) Date: Wed May 23 14:50:35 2007 Subject: [Histonet] NCSHT Symposia Duke University June 19th 2007 Message-ID: <8C96B8ABB77D86D-1084-25A2@FWM-M06.sysops.aol.com> ?NCSHT Symposia Duke University? Durham, NC ? June 19th 2007 ? ?Registration/ 8:30 AM ?"Histology - The Evolution of Automation" Speaker Carolyn Doan, Leica Microsystems? ?Complimentary Breakfast ?"Optimizing Tissue Processing" Speaker Matthew Hoskin/TBD, Vision BioSystems? ?"Are Your CDs Earning Interests?" Guest Speaker Lamar Jones, Wake Forest University/Baptist Medical Center ?Registration/ 8:30 AM ?"Histology - The Evolution of Automation" Speaker Carolyn Doan, Leica Microsystems? ?Complimentary Breakfast ?"Optimizing Tissue Processing" Speaker Matthew Hoskin/TBD, Vision BioSystems? ?"Are Your CDs Earning Interests?" Guest Speaker Lamar Jones, Wake Forest University/Baptist Medical Center ? Complimentary Luncheon ? "IHC and ISH: Maximizing Quality and Workflow" Speaker Matthew Hoskin/TBD, Vision BioSystems ?AFTERNOON TEA ?"Use of IHC to Expedite Cancer Drug Discovery" Guest Speaker Ryan Williams, MD Anderson Cancer Center ?"Special Immunohistochemical Applications" Guest Speaker Jim Burchette, Duke University Medical Center ?Wine and Cheese Reception ?Slide Review and Open Discussion ? Cost:? NCSHT asks you to donate a new teddy bear (or other stuffed animal) or children?s book to our children?s hospital charity drive as your admission cost for this event.? Registration: Contact Wendy Gibson at:? wendy.gibson@vision-bio.com or (781) 616-1245 by June 8, 2007 Registration: Contact Wendy Gibson at:? wendy.gibson@vision-bio.com or (781) 616-1245 by June 8, 2007 ?Warmest Regards Wanda ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From garret.t.miyamoto <@t> us.army.mil Wed May 23 15:00:25 2007 From: garret.t.miyamoto <@t> us.army.mil (garret.t.miyamoto@us.army.mil) Date: Wed May 23 15:00:33 2007 Subject: [Histonet] Re: Histonet Digest, Vol 42, Issue 33 In-Reply-To: <5s9rj3$kehs4@mxoutps1.us.army.mil> References: <5s9rj3$kehs4@mxoutps1.us.army.mil> Message-ID: ----- Original Message ----- From: histonet-request@lists.utsouthwestern.edu Date: Wednesday, May 23, 2007 7:13 am Subject: Histonet Digest, Vol 42, Issue 33 To: histonet@lists.utsouthwestern.edu > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: Histonet Digest, Vol 42, Issue 32 (Kobler, James) > 2. Ethanol ready-to-use for LCM (Castillos, Luminita) > 3. RE: NK cells in mouse tissue and PLP fixation (Gayle Callis) > 4. RE: -20C acetone fixative (C.M. van der Loos) > 5. RE: RE: -20C acetone fixative (Edwards, R.E.) > 6. Cryomold adapters (Martha Ward) > 7. Cell Blocks on small buttons... (Lott, Robert) > 8. celloidin-ether/alc (Mary Lou Norman) > 9. Work hours (Patti Loykasek) > 10. RE: celloidin-ether/alc (Rittman, Barry R) > 11. Re: Work hours (Rene J Buesa) > 12. RE: Work hours (Mike Pence) > > > ------------------------------------------------------------------- > --- > > Message: 1 > Date: Wed, 23 May 2007 10:20:34 -0400 > From: "Kobler, James" > Subject: [Histonet] RE: Histonet Digest, Vol 42, Issue 32 > To: > Message-ID: > <6D1DFC2837CEAE4BBBDED596071430854D07D4@PHSXMB4.partners.org> > Content-Type: text/plain; charset="us-ascii" > > Dear histonet contributors, > > I would greatly appreciate any advice about antibodies that work > well with > ferret tissue (and any related processing tips). We are > particularly interested > in antibodies to markers for fibroblasts, myofibroblasts, > endothelial, smooth > muscle and inflammatory cells, as well as extracellular matrix. > Thanks very > much, > > Jim Kobler, Massachusetts General Hospital > > > > > > The information transmitted in this electronic communication is > intended only for the person or entity to whom it is addressed and > may contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of or taking of any > action in reliance upon this information by persons or entities > other than the intended recipient is prohibited. If you received > this information in error, please contact the Compliance HelpLine > at 800-856-1983 and properly dispose of this information. > > > > > ------------------------------ > > Message: 2 > Date: Wed, 23 May 2007 10:28:19 -0400 > From: "Castillos, Luminita" > Subject: [Histonet] Ethanol ready-to-use for LCM > To: > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > > Hi, > > Does any know from where to buy ready-to-use ethanol (100%, 95%, 70%) > molecular biology grade and RNase-free, compatible for Laser Capture > microdissection staining protocol?. Thank you very much. > > L, > > > ------------------------------ > > Message: 3 > Date: Wed, 23 May 2007 08:43:03 -0600 > From: Gayle Callis > Subject: RE: [Histonet] NK cells in mouse tissue and PLP fixation > To: Bruce Abaloz , > Histonet@lists.utsouthwestern.edu > Message-ID: > <6.0.0.22.1.20070523083057.01b3a148@gemini.msu.montana.edu> > Content-Type: text/plain; charset="us-ascii"; format=flowed > > Sonya, > > You can try PLP immersion fixation followed by sucrose > cryoprotection, but > the publication I read (reference was in a Histonet message > yesterday) did > a fixation study with PLP and other fixatives. They had little > success > with the NK1.1 (P136) antibody following immersion fixation with > all > fixatives, before snap freezing or after fresh tissue frozen > sections were > cut. They had nice staining following PERFUSION (with PLP) of the > mouse. After perfusion, and once the tissues are dissected out, > then > immersion into this fixative should ensure complete fixation. You > may want > to do final fixation in PLP overnight, some do it for 5 - 6 hours > longer > here, then do the 30% sucrose cryoprotection at 4C. Just remove > the tissue > from fixative and go to the sucrose solution as Bruce suggested. > Small > fixed tissues often float on top of the sucrose solution. A > general rule > of thumb the that cryoprotection is done, tissues sink to the > bottom of the > container. > > Some people use a sucrose gradient, often seen in the literature, > but we > have never found that necessary. We blot off the excess sucrose > solution > before embedding in OCT. > > Hopefully you have access to a perfusion methods IF your immersion > fails. > Gayle Callis HTL, HT, MT(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University > Bozeman MT 59717 > > > At 06:12 PM 5/22/2007, you wrote: > >I just snap frooze the fresh tissue in isopentane on dry ice - > this has > >worked with all the other markers I've been looking at but I > gather NK > >cells are more tricky. > >I want to try PLP fixation before I freeze so do I put it > straight from > >PLP to 30% sucrose or do I wash/use increasing amounts of sucrose. > >Once I've left the tissue overnight in sucrose can I then freeze > it in > >TissueTek on dry ice as normal? > > > >Thanks > >Sonya > > > >-----Original Message----- > >From: Gayle Callis [mailto:gcallis@montana.edu] > >Sent: 22 May 2007 15:49 > >To: Martin S. > >Subject: Re: [Histonet] NK cells in mouse tissue and PLP fixation > > > >How did you fix in the first place? > > > >You need to sucrose cryoprotect after PLP fixation, in 30% sucrose > >overnight BEFORE snap freezing the tissues. > > > >At 04:01 AM 5/22/2007, you wrote: > >>Has anyone done any staining for NK cells in mouse tissue. We > have an > >>antibody against NK1.1 (PK136) but it doesnt seem to work on > >>fresh-frozen tissues. I have looked through the literature and some > >>people seem to have got iot to work on fresh-frozens while > others have > >>used PLP fixation before embedding in Tissue Tek. > >> > >>Has anyone had any experience with this Ab? > >> > >>Also, if I fix the tissues with PLP before freezing then how > should I > >>treat them before outting them in TissueTek to freeze? > >> > >>Thanks > >>Sonya > >> > >> > >> > >>_______________________________________________ > >>Histonet mailing list > >>Histonet@lists.utsouthwestern.edu > >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >Gayle Callis > >MT,HT,HTL(ASCP) > >Research Histopathology Supervisor > >Veterinary Molecular Biology > >Montana State University - Bozeman > >PO Box 173610 > >Bozeman MT 59717-3610 > > > > > > > > > > > > > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >-- > >BRUCE ABALOZ > >HISTOLOGIST PH:61383446282 > >DEPT. of ZOOLOGY EMAIL: brucea@unimelb.edu.au > >THE UNIVERSITY of MELBOURNE. FAX:61383447909 > >VICTORIA. AUSTRALIA 3010 > > Nobody Can Make You Feel Inferior Without YOUR > Permission - > > Eleanor Roosevelt > > WHETHER YOU THINK YOU CAN-OR WHETHER YOU > THINK YOU > > CAN'T-YOU'RE RIGHT!! > > Be reasonable... > Demand the > > impossible..... > > <')))>>< <')))>>< > > <')))>>< <')))>>< > > P Please consider the environment before printing > this e-mail. > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 4 > Date: Wed, 23 May 2007 16:48:47 +0200 > From: "C.M. van der Loos" > Subject: [Histonet] RE: -20C acetone fixative > To: histonet@lists.utsouthwestern.edu > Message-ID: <37e62d383380.38338037e62d@amc.uva.nl> > Content-Type: text/plain; charset="us-ascii" > > > Long time ago they told me it is a milder fixative at -20C > than at RT > ??? > > Chris van der Loos, PhD > Dept. of Pathology > Academic Medical Center M2-230 > Meibergdreef 9 > NL-1105 AZ Amsterdam > The Netherlands > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [[1]mailto:histonet-bounces@lists.utsouthwestern.edu] On > Behalf Of > Charles, > Roger > Sent: Tuesday, May 22, 2007 12:29 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] -20C acetone fixative > Does any know or remember why acetone, when used as a fixative, is > always used "ice cold" or in our case at -20C? > Roger Charles > Microbiologist > Pennsylvania Veterinary Laboratory > 2305 N Cameron St > Harrisburg, PA 17110 > 717-787-8808 > > References > > 1. javascript:main.compose('new', 't=histonet- > bounces@lists.utsouthwestern.edu') > > ------------------------------ > > Message: 5 > Date: Wed, 23 May 2007 16:15:14 +0100 > From: "Edwards, R.E." > Subject: RE: [Histonet] RE: -20C acetone fixative > To: "C.M. van der Loos" , > > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > And me, in particular for enzyme histochemistry... > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > C.M. van > der Loos > Sent: 23 May 2007 15:49 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: -20C acetone fixative > > > Long time ago they told me it is a milder fixative at -20C > than at > RT > ??? > > Chris van der Loos, PhD > Dept. of Pathology > Academic Medical Center M2-230 > Meibergdreef 9 > NL-1105 AZ Amsterdam > The Netherlands > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [[1]mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf > Of > Charles, > Roger > Sent: Tuesday, May 22, 2007 12:29 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] -20C acetone fixative > Does any know or remember why acetone, when used as a fixative, is > always used "ice cold" or in our case at -20C? > Roger Charles > Microbiologist > Pennsylvania Veterinary Laboratory > 2305 N Cameron St > Harrisburg, PA 17110 > 717-787-8808 > > References > > 1. javascript:main.compose('new', > 't=histonet-bounces@lists.utsouthwestern.edu') > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 6 > Date: Wed, 23 May 2007 11:02:18 -0400 > From: "Martha Ward" > Subject: [Histonet] Cryomold adapters > To: > Message-ID: > <61135F0455D33347B5AAE209B903A3041B1C9EBD@EXCHVS2.medctr.ad.wfubmc.edu> > > Content-Type: text/plain; charset="us-ascii" > > I am looking for cryomold adapters and I hope someone can help. The > ones we are using have a round center and are a white, hard > plastic. I > can find adapters that have a square center but that isn't what we > want.I have tried the usual vendors but was hoping someone could > help me. > Thanks in advance for your help. > > Martha Ward, MT (ASCP) QIHC > Assistant Manager, Molecular Diagnostics Lab > Wake Forest University Baptist Medical Center > Medical Center Blvd. > Winston-Salem, NC 27157 > 336-716-2756 > mward@wfubmc.edu > > > > ------------------------------ > > Message: 7 > Date: Wed, 23 May 2007 10:23:14 -0500 > From: "Lott, Robert" > Subject: [Histonet] Cell Blocks on small buttons... > To: > Message-ID: > <673832E27C45FC4D97EF758FFC777C27020E03DC@CPRTEVS01.triadhospitals.net> > > Content-Type: text/plain; charset="us-ascii" > > Hi Everyone, > > We are interested in hearing about different procedures for recovering > small cytology aspirates (any fluid really!) ... and making a cell > blockfrom them using some type of coagulative process/fluid/etc. > ... so that > virtually all of the cells are retained! > > > > There may be some commercial products available ... would like to hear > about those as well.!! > > > > Thanks!!!! > > > > Robert > > > > Robert L. Lott, HTL(ASCP) > > Manager, Anatomic Pathology > > LabFirst / Trinity Medical Center - formerly > > Montclair Baptist Medical Center > > 800 Montclair Road > > Birmingham, AL 35213 > > 205-592-5388 phone > > 205-592-5646 fax > > robert.lott@triadhospitals.com > > > > > > > ------------------------------ > > Message: 8 > Date: Wed, 23 May 2007 11:26:13 -0400 > From: Mary Lou Norman > Subject: [Histonet] celloidin-ether/alc > To: histonet@lists.utsouthwestern.edu > Message-ID: > <6.2.1.2.2.20070523111029.04899930@postoffice9.mail.cornell.edu> > Content-Type: text/plain; charset="us-ascii"; format=flowed > > Hello Histonet, > > Is there any other choice besides the cellodin-ether/alcohol > coating? My > parloidin is taking 'forever' to dissolve -- I have read a week > but I could > have used it a week ago. > > Thanks for your help. > Mary Lou Norman > > > > > ------------------------------ > > Message: 9 > Date: Wed, 23 May 2007 08:42:22 -0700 > From: Patti Loykasek > Subject: [Histonet] Work hours > To: histonet > Message-ID: > Content-Type: text/plain; charset="US-ASCII" > > I was wondering if anyone in histoland has experienced moving from a > schedule of standard 8 hour day/5 days a week to a 10 hour day/4 > days a > week with techs having a day off during the week. If so, what are > the pros > and cons of this type of schedule. I'm interested in feedback from > bothbench techs and supervisor/managers. We are in the initial > stages of > contemplating this and would appreciate info from anyone that has > tried it. > Thank you. > > > Patti Loykasek BS, HTL, QIHC > PhenoPath Laboratories > Seattle, WA > > > > ------------------------------------------------------------------- > ------ > This e-mail message, including any attachments, is for the sole > use of > the intended recipients and may contain privileged information. > Any > unauthorized review, use, disclosure or distribution is > prohibited. If > you are not the intended recipient, please contact the sender by e- > mail > and destroy all copies of the original message, or you may call > PhenoPath > Laboratories, Seattle, WA U.S.A. at (206) 374-9000. > > > > > ------------------------------ > > Message: 10 > Date: Wed, 23 May 2007 11:37:45 -0500 > From: "Rittman, Barry R" > Subject: RE: [Histonet] celloidin-ether/alc > To: "Mary Lou Norman" , > > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > Mary Lou > Will dissolve much more rapidly if soak the strips first in > absolute ethanol for a day o so. The add the ether. > Barry > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Mary > Lou Norman > Sent: Wed 5/23/2007 10:26 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] celloidin-ether/alc > > > > Hello Histonet, > > Is there any other choice besides the cellodin-ether/alcohol > coating? My > parloidin is taking 'forever' to dissolve -- I have read a week > but I could > have used it a week ago. > > Thanks for your help. > Mary Lou Norman > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 11 > Date: Wed, 23 May 2007 09:52:11 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Work hours > To: Patti Loykasek , histonet > > Message-ID: <990345.12651.qm@web61216.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > I have tried it. > Cons: If yu don't have a flow of specimens in 4 days equivalent > to the flow on 5 days, the personnel will be idling some time each > day. If specimens or workload is available everybody will have > work to do. > Pros: it is fantastic having 3 days off weekly. Everybody likes > that! Ren? J. > > Patti Loykasek wrote: > I was wondering if anyone in histoland has experienced moving > from a > schedule of standard 8 hour day/5 days a week to a 10 hour day/4 > days a > week with techs having a day off during the week. If so, what are > the pros > and cons of this type of schedule. I'm interested in feedback from > bothbench techs and supervisor/managers. We are in the initial > stages of > contemplating this and would appreciate info from anyone that has > tried it. > Thank you. > > > Patti Loykasek BS, HTL, QIHC > PhenoPath Laboratories > Seattle, WA > > > > ------------------------------------------------------------------- > ------ > This e-mail message, including any attachments, is for the sole > use of > the intended recipients and may contain privileged information. > Any > unauthorized review, use, disclosure or distribution is > prohibited. If > you are not the intended recipient, please contact the sender by e- > mail > and destroy all copies of the original message, or you may call > PhenoPath > Laboratories, Seattle, WA U.S.A. at (206) 374-9000. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Looking for a deal? Find great prices on flights and hotels with > Yahoo! FareChase. > > ------------------------------ > > Message: 12 > Date: Wed, 23 May 2007 11:58:01 -0500 > From: "Mike Pence" > Subject: RE: [Histonet] Work hours > To: "Rene J Buesa" , "Patti Loykasek" > , "histonet" > Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C5E3@IS-E2K3.grhs.net> > Content-Type: text/plain; charset="iso-8859-1" > > How do you decide who gets which days off! Are they floating days? > > Mike > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Wednesday, May 23, 2007 11:52 AM > To: Patti Loykasek; histonet > Subject: Re: [Histonet] Work hours > > > I have tried it. > Cons: If yu don't have a flow of specimens in 4 days equivalent > to the flow on 5 days, the personnel will be idling some time each > day. If specimens or workload is available everybody will have > work to do. > Pros: it is fantastic having 3 days off weekly. Everybody likes > that! Ren? J. > > Patti Loykasek wrote: > I was wondering if anyone in histoland has experienced moving > from a schedule of standard 8 hour day/5 days a week to a 10 hour > day/4 days a week with techs having a day off during the week. If > so, what are the pros and cons of this type of schedule. I'm > interested in feedback from both bench techs and > supervisor/managers. We are in the initial stages of contemplating > this and would appreciate info from anyone that has tried it. > Thank you. > > > Patti Loykasek BS, HTL, QIHC > PhenoPath Laboratories > Seattle, WA > > > > ------------------------------------------------------------------- > ------ > This e-mail message, including any attachments, is for the sole > use of the intended recipients and may contain privileged > information. Any > unauthorized review, use, disclosure or distribution is > prohibited. If > you are not the intended recipient, please contact the sender by e- > mail > and destroy all copies of the original message, or you may call > PhenoPath > Laboratories, Seattle, WA U.S.A. at (206) 374-9000. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > --------------------------------- > Looking for a deal? Find great prices on flights and hotels with > Yahoo! FareChase. _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 42, Issue 33 > **************************************** > Re: cell blocks on small buttons Robert, I asked someone in our cytology section about your inquiry. What they do is spin down the specimen in a centrifuge, decant the liquid, add about four drops of "HistoGel" (specimen processing gel from Richard-Allen Scientific, 12 vials - 10 ml per vial, reorder number HG 4000-012), respin the specimen and collect the gel button, put it into a tissue specimen bag before placing into the cassette. I hope this will work for you. Garret From ploykasek <@t> phenopath.com Wed May 23 15:59:43 2007 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Wed May 23 16:00:26 2007 Subject: [Histonet] Work hours Message-ID: Just wanted to say thanks to everyone for their replies about the 10hr/4day work schedule. I appreciate everyone taking the time to reply. The replies have given me some things to think about and investigate. If we do go forward with this, I will keep the histonet informed as to our experience. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From FUNKM <@t> mercyhealth.com Wed May 23 16:03:46 2007 From: FUNKM <@t> mercyhealth.com (Marcia Funk) Date: Wed May 23 16:04:12 2007 Subject: [Histonet] salary range Message-ID: <465465E2020000AC0000289B@nodcmsngwia1.trinity-health.org> Hello, I know we have talked about the salary range before and the NSH has information for there last servey which is dated 2005. I'm working hard with management to upgrade the pay grade for Histology. If you have some information it would be greatly appreciated. Hospital size 309 beds, 23,0000 surgicals, autopsy and cellblocks. We do specials and a full range of IHC and ISH. We are in the midwest, working 8 hours shifts starting at 5:30. My concern is that there are labs that just do Histology and then have a Special/IHC lab, where as our lab is a full service lab. Any thoughts would be greatly appreciated. Marcia From mickie25 <@t> netzero.net Wed May 23 21:16:54 2007 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Wed May 23 21:17:14 2007 Subject: [Histonet] Mohs Position Available in the North East In-Reply-To: References: Message-ID: Hello everyone. There is a Mohs histology position available in Rhode Island. Anyone interested, please contact me and I will fill in details. Thank you. Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net ____________________________________________________________________________ __ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ljwh2 <@t> cam.ac.uk Thu May 24 04:23:03 2007 From: ljwh2 <@t> cam.ac.uk (Laura Harris) Date: Thu May 24 04:23:13 2007 Subject: [Histonet] Re: Ethanol ready-to-use for LCM Message-ID: <46555977.50401@cam.ac.uk> Hi Luminita Ambion might sell this, if they don't then it is probably not available as a specific product. RNases do not generally survive in 100% ethanol so the ethanol itself does not need to be special. We use the PALM system and have had good results making up our own ethanols with RNase-free water. Cheers, Laura Institute of Biotechnology, University of Cambridge, UK. Date: Wed, 23 May 2007 10:28:19 -0400 > From: "Castillos, Luminita" > Subject: [Histonet] Ethanol ready-to-use for LCM > To: > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > > Hi, > > Does any know from where to buy ready-to-use ethanol (100%, 95%, 70%) > molecular biology grade and RNase-free, compatible for Laser Capture > microdissection staining protocol?. Thank you very much. > > L, From louise.renton <@t> gmail.com Thu May 24 04:40:38 2007 From: louise.renton <@t> gmail.com (louise renton) Date: Thu May 24 04:40:42 2007 Subject: [Histonet] cathepsin K Message-ID: Hi all, anybody using BioVision's Cath K for manual staining on paraffin wax sections. If so - what dilutions are you having best results with? ( I am only asking cos time is limited - I have to present data next week, and the Ab has just arrived. Otherwise I would do my own optimization) As always, many many thanks -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From kmerriam2003 <@t> yahoo.com Thu May 24 07:13:39 2007 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Thu May 24 07:13:44 2007 Subject: [Histonet] Ethanol ready-to-use for LCM Message-ID: <873647.52467.qm@web50303.mail.re2.yahoo.com> Hello, You can purchase an entire staining kit, which includes all of the ethanol and xylene (as well as a small vial of mystery dye) from Arcturus. I don't think they sell the ethanols alone, but you will need just about everything in the kit anyway. Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA ----- Original Message ---- From: "Castillos, Luminita" To: histonet@lists.utsouthwestern.edu Sent: Wednesday, May 23, 2007 10:28:19 AM Subject: [Histonet] Ethanol ready-to-use for LCM Hi, Does any know from where to buy ready-to-use ethanol (100%, 95%, 70%) molecular biology grade and RNase-free, compatible for Laser Capture microdissection staining protocol?. Thank you very much. L, _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ TV dinner still cooling? Check out "Tonight's Picks" on Yahoo! TV. http://tv.yahoo.com/ From rjbuesa <@t> yahoo.com Thu May 24 08:39:05 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu May 24 08:39:17 2007 Subject: [Histonet] salary range In-Reply-To: <465465E2020000AC0000289B@nodcmsngwia1.trinity-health.org> Message-ID: <108208.58122.qm@web61211.mail.yahoo.com> Marcia: I will tell you what I did twice: 1- I called all hospitals in my area and found out their salary ranges; 2- with that information and anlyzing the training and educational background of my staff, I prepared a really fair salary scale/range; and 3- I presented it to management to discuss it. Twice I succeeded in changing the salary range at my lab. Please remember that salary (as politics) is a local thing and in salary is more local, is "laboratory based" and offer/demand rules in many cases. Forget about what is paid in your region or even in the State. Go local, very local, to the other hospitals in the area that could be the ones that could "lure" your best techs by offering a better salary. Local competintion and availability of histotechs is what should be taken into considerations. Hope this will help you! Ren? J. Marcia Funk wrote: Hello, I know we have talked about the salary range before and the NSH has information for there last servey which is dated 2005. I'm working hard with management to upgrade the pay grade for Histology. If you have some information it would be greatly appreciated. Hospital size 309 beds, 23,0000 surgicals, autopsy and cellblocks. We do specials and a full range of IHC and ISH. We are in the midwest, working 8 hours shifts starting at 5:30. My concern is that there are labs that just do Histology and then have a Special/IHC lab, where as our lab is a full service lab. Any thoughts would be greatly appreciated. Marcia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better Heartthrob. Get better relationship answers from someone who knows. Yahoo! Answers - Check it out. From rachael_emerson <@t> urmc.rochester.edu Thu May 24 09:29:38 2007 From: rachael_emerson <@t> urmc.rochester.edu (Rachael Emerson) Date: Thu May 24 09:29:59 2007 Subject: [Histonet] p53 Message-ID: Hello. I am working with a mouse anti-p53 monoclonal antibody (PAb421) from Calbiochem (#OP03L) and I am having problems with endogenous mouse background. The tissue that I am staining is irradiated mouse spleen, mouse fetal liver, and adult mouse bone marrow. I am currently blocking with peroxide, avidin/biotin block from Vector, levamisole, and using Vector?s Mouse-on-Mouse Immunodectection Kit. Unfortunately I am still getting background staining on my no primary controls-even with all of the blocking steps. I would really appreciate any thoughts or suggestions. Thanks! Rachael Emerson -- Rachael L. Emerson Center for Pediatric Biomedical Research University of Rochester Medical Center 575 Elmwood Avenue MRBX 1.11301 Rochester, NY 14642 From anitathorn <@t> comcast.net Thu May 24 09:57:51 2007 From: anitathorn <@t> comcast.net (anitathorn@comcast.net) Date: Thu May 24 09:58:03 2007 Subject: [Histonet] Tissue Tek Cryostat Message-ID: <052420071457.18086.4655A7EF0008EA39000046A62207020853029D01089B0E9B07020E@comcast.net> Karen, I have an old Tissue Tek II as the "back-up" cryostat. I monitor its temperature everyday and found that the temperature I have it set at and the actual temp varies considerably. I had our bio-med guy look at it and he said that there really isn't any thing he could do about it. So I watch it closely. I noticed that as frost starts to build up, the tempreature goes up. I know it's time to defrost it when the temp gets out of range. Once newly defrosted, it goes back to the colder end of the range. I have a seperate thermometer inside the chamber to get an acurate reading for my QA log. I do have a manual (copyright 1966!!) and it doen't say anything about setting the temperature. Hope this helps, Anita ------- Original message -------------- From: "Heckford, Karen - SMMC-SF" > Good Morning Everyone, We have a old Tissue Tek 11 4553 cryostat. We > noticed after defrosting it that the temperature went way cold. We turned > the dial which moves the black hand and set it for -25 and noticed that the > blue hand is way past -30. We cannot find the users guide to save our > lives. Probably lost a long time ago. Anyways can anyone tell me how to > reset this thing. > Cheers, > > Karen Heckford HT (ASCP) CE > Lead Histology Technician > Histology/Pathology Department > St. Mary's Medical Center > 450 Stanyan St. > San Francisco, Ca. 94117 > 415-668-1000 ext. 6167 > Fax: 415-750-8123 > email: kheckfor@chw.edu From jgutierrez <@t> precisionpath.us Thu May 24 10:03:23 2007 From: jgutierrez <@t> precisionpath.us (Juan Gutierrez) Date: Thu May 24 10:04:09 2007 Subject: [Histonet] salary range In-Reply-To: <108208.58122.qm@web61211.mail.yahoo.com> References: <465465E2020000AC0000289B@nodcmsngwia1.trinity-health.org> <108208.58122.qm@web61211.mail.yahoo.com> Message-ID: Amen brother! You hit the nail in the head when you mentioned who your competition really is. It will be the local labs that WILL steal your techs away. Look at me. I'm at a third different job this year, because I got tired of fighting management about salaries for my techs and doing my best to keep them. Now I'm just another hired gun looking out for number one. Good luck! Juan C. Gutierrez, HT(ASCP) Just a Histo Tech again and loving it!!! Precision Pathology Services (210)646-0890 Ext.203 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, May 24, 2007 8:39 AM To: Marcia Funk; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] salary range Marcia: I will tell you what I did twice: 1- I called all hospitals in my area and found out their salary ranges; 2- with that information and anlyzing the training and educational background of my staff, I prepared a really fair salary scale/range; and 3- I presented it to management to discuss it. Twice I succeeded in changing the salary range at my lab. Please remember that salary (as politics) is a local thing and in salary is more local, is "laboratory based" and offer/demand rules in many cases. Forget about what is paid in your region or even in the State. Go local, very local, to the other hospitals in the area that could be the ones that could "lure" your best techs by offering a better salary. Local competintion and availability of histotechs is what should be taken into considerations. Hope this will help you! Ren? J. Marcia Funk wrote: Hello, I know we have talked about the salary range before and the NSH has information for there last servey which is dated 2005. I'm working hard with management to upgrade the pay grade for Histology. If you have some information it would be greatly appreciated. Hospital size 309 beds, 23,0000 surgicals, autopsy and cellblocks. We do specials and a full range of IHC and ISH. We are in the midwest, working 8 hours shifts starting at 5:30. My concern is that there are labs that just do Histology and then have a Special/IHC lab, where as our lab is a full service lab. Any thoughts would be greatly appreciated. Marcia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better Heartthrob. Get better relationship answers from someone who knows. Yahoo! Answers - Check it out. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Thu May 24 10:04:50 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu May 24 10:04:49 2007 Subject: [Histonet] Re: Ethanol ready-to-use for LCM In-Reply-To: <46555977.50401@cam.ac.uk> References: <46555977.50401@cam.ac.uk> Message-ID: <6.0.0.22.1.20070524090226.01b6b578@gemini.msu.montana.edu> A LCM expert colleague of mine prepares her lower concentration ethanols the same way at Laura. At 03:23 AM 5/24/2007, you wrote: >Hi Luminita > >Ambion might sell this, if they don't then it is probably not available as >a specific product. RNases do not generally survive in 100% ethanol so the >ethanol itself does not need to be special. We use the PALM system and >have had good results making up our own ethanols with RNase-free water. > >Cheers, Laura Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 From emerald_lake77 <@t> yahoo.com Thu May 24 10:12:20 2007 From: emerald_lake77 <@t> yahoo.com (GT Hebert) Date: Thu May 24 10:12:27 2007 Subject: [Histonet] Nikon NIS-Elements - digital camera software problem; software others use?? Message-ID: <727304.65079.qm@web31702.mail.mud.yahoo.com> Hello, Is anyone out there using a Nikon camera with NIS-elements (Advanced Research) software for digital imaging capture and digital image analysis?? Since buying the microscope system, I have been experiencing a multitude of software problems. In particular, during image 'capture', the computer will crash - a blue screen with white script would pop up and the computer subsequently will restart itself. Upon trying to open the software again, an error message would come up. I would have to restart the computer manually and open the software again. Nikon has come up with higher versions of the software and the updates have been installed, but as of yet, the problem has not been fixed. So my questions are as follows: Do you currently us NIS-Elements? If so, have you experienced similar issues? What image capturing software is the BEST both in terms of analysis and image capture? If I have to, I will eventually move on to a better system if this one continues to give me problems. Your answers and insight is much appreciated. Thank you in advance. Gustave Hebert Scientist II Wyeth Research Cambridge MA --------------------------------- 8:00? 8:25? 8:40? Find a flick in no time with theYahoo! Search movie showtime shortcut. From gcallis <@t> montana.edu Thu May 24 10:18:24 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu May 24 10:18:22 2007 Subject: [Histonet] Tissue Tek Cryostat In-Reply-To: <052420071457.18086.4655A7EF0008EA39000046A62207020853029D0 1089B0E9B07020E@comcast.net> References: <052420071457.18086.4655A7EF0008EA39000046A62207020853029D01089B0E9B07020E@comcast.net> Message-ID: <6.0.0.22.1.20070524091051.01b08830@gemini.msu.montana.edu> A little trick to keep frost buildup at a minimum in an old Tissue Tek II cryostat. After defrosting, we used to wipe down the flat metal surfaces of chamber with ethylene glycol, or antifreeze, leaving a bit of this behind on the surface. This kept frost from forming so fast, but do NOT get this on knife holder or blade or frozen blocks, you do not want defrosted frozen sections. Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 At 08:57 AM 5/24/2007, you wrote: >Karen, >I have an old Tissue Tek II as the "back-up" cryostat. I monitor its >temperature everyday and found that the temperature I have it set at and >the actual temp varies considerably. I had our bio-med guy look at it and >he said that there really isn't any thing he could do about it. So I watch >it closely. I noticed that as frost starts to build up, the tempreature >goes up. I know it's time to defrost it when the temp gets out of >range. Once newly defrosted, it goes back to the colder end of the >range. I have a seperate thermometer inside the chamber to get an acurate >reading for my QA log. I do have a manual (copyright 1966!!) and it >doen't say anything about setting the temperature. >Hope this helps, >Anita >------- Original message -------------- >From: "Heckford, Karen - SMMC-SF" > > > Good Morning Everyone, We have a old Tissue Tek 11 4553 cryostat. We > > noticed after defrosting it that the temperature went way cold. We turned > > the dial which moves the black hand and set it for -25 and noticed that > the > > blue hand is way past -30. We cannot find the users guide to save our > > lives. Probably lost a long time ago. Anyways can anyone tell me how to > > reset this thing. > > Cheers, > > > > Karen Heckford HT (ASCP) CE > > Lead Histology Technician > > Histology/Pathology Department > > St. Mary's Medical Center > > 450 Stanyan St. > > San Francisco, Ca. 94117 > > 415-668-1000 ext. 6167 > > Fax: 415-750-8123 > > email: kheckfor@chw.edu _______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From danarobinmarshal <@t> aol.com Thu May 24 10:18:44 2007 From: danarobinmarshal <@t> aol.com (danarobinmarshal@aol.com) Date: Thu May 24 10:18:53 2007 Subject: [Histonet] (no subject) Message-ID: <8C96C2DF3B38D4D-D60-B78@webmail-de15.sysops.aol.com> Hi all, We are going to try the Abcam NEDL2 antibody (ab13821) for IHC.? According to the Abcam site it has not been tested for IHC on any type of tissue.? Does anyone have any experience?? If so, what tissue and whether it was FFPE or fresh frozen (and all other details if possible!)? Thanks a lot, dana marshall (meharry medical college, Nashville TN) ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From brett_connolly <@t> merck.com Thu May 24 10:18:46 2007 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Thu May 24 10:19:16 2007 Subject: [Histonet] Nikon NIS-Elements - digital camera software problem; software others use?? In-Reply-To: <727304.65079.qm@web31702.mail.mud.yahoo.com> References: <727304.65079.qm@web31702.mail.mud.yahoo.com> Message-ID: <63EA0607835FBA4689CEA9EA8B4826922AB398@usctmx1141.merck.com> Gustave, I use NIS-Elements and have not experienced any of those problems. Ours was installed earlier this year. Brett M. Connolly, Ph.D. Research Fellow MRL, Imaging Research Merck & Co., Inc. WP-44K PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of GT Hebert Sent: Thursday, May 24, 2007 11:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Nikon NIS-Elements - digital camera software problem; software others use?? Hello, Is anyone out there using a Nikon camera with NIS-elements (Advanced Research) software for digital imaging capture and digital image analysis?? Since buying the microscope system, I have been experiencing a multitude of software problems. In particular, during image 'capture', the computer will crash - a blue screen with white script would pop up and the computer subsequently will restart itself. Upon trying to open the software again, an error message would come up. I would have to restart the computer manually and open the software again. Nikon has come up with higher versions of the software and the updates have been installed, but as of yet, the problem has not been fixed. So my questions are as follows: Do you currently us NIS-Elements? If so, have you experienced similar issues? What image capturing software is the BEST both in terms of analysis and image capture? If I have to, I will eventually move on to a better system if this one continues to give me problems. Your answers and insight is much appreciated. Thank you in advance. Gustave Hebert Scientist II Wyeth Research Cambridge MA --------------------------------- 8:00? 8:25? 8:40? Find a flick in no time with theYahoo! Search movie showtime shortcut. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------------ From FUNKM <@t> mercyhealth.com Thu May 24 10:27:23 2007 From: FUNKM <@t> mercyhealth.com (Marcia Funk) Date: Thu May 24 10:28:02 2007 Subject: [Histonet] Salary range Message-ID: <4655688B020000AC000028D1@nodcmsngwia1.trinity-health.org> Hello, One thing I didn't add is that our hospital is a reference hospital where as we receive specimens in from Hospitals and Clinics that do not have a Histology lab . The largest hospital that we would compete with is 3 hours away. We have been together as a team for 10 years to 25 years so as you can see we have a great team that works well together with great knowledge and productivity. Marcia From rjbuesa <@t> yahoo.com Thu May 24 10:30:58 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu May 24 10:31:06 2007 Subject: [Histonet] Salary range In-Reply-To: <4655688B020000AC000028D1@nodcmsngwia1.trinity-health.org> Message-ID: <294323.8664.qm@web61225.mail.yahoo.com> OK, so let them pay for your reliable team! Ren? J. Marcia Funk wrote: Hello, One thing I didn't add is that our hospital is a reference hospital where as we receive specimens in from Hospitals and Clinics that do not have a Histology lab . The largest hospital that we would compete with is 3 hours away. We have been together as a team for 10 years to 25 years so as you can see we have a great team that works well together with great knowledge and productivity. Marcia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ready for the edge of your seat? Check out tonight's top picks on Yahoo! TV. From danarobinmarshal <@t> aol.com Thu May 24 10:33:38 2007 From: danarobinmarshal <@t> aol.com (danarobinmarshal@aol.com) Date: Thu May 24 10:33:47 2007 Subject: [Histonet] Abcam NEDL2 antibody (ab13821) for IHC In-Reply-To: <8C96C2DF3B38D4D-D60-B78@webmail-de15.sysops.aol.com> References: <8C96C2DF3B38D4D-D60-B78@webmail-de15.sysops.aol.com> Message-ID: <8C96C3008C9D933-18CC-CE6@FWM-M12.sysops.aol.com> sorry, I committed the sin of no subject in my previous e-mail, i am rusty, here it is again. -----Original Message----- From: danarobinmarshal@aol.com To: Histonet@lists.utsouthwestern.edu; cjones@mmc.edu Sent: Thu, 24 May 2007 10:18 am Subject: [Histonet] (no subject) Hi all, We are going to try the Abcam NEDL2 antibody (ab13821) for IHC.? According to he Abcam site it has not been tested for IHC on any type of tissue.? Does nyone have any experience?? If so, what tissue and whether it was FFPE or fresh rozen (and all other details if possible!)? Thanks a lot, dana marshall (meharry medical college, Nashville TN) ________________________________________________________________________ OL now offers free email to everyone. Find out more about what's free from AOL t AOL.com. ______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From mcauliff <@t> umdnj.edu Thu May 24 10:45:18 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu May 24 10:58:50 2007 Subject: [Histonet] telephone solicitations Message-ID: <4655B30E.6030502@umdnj.edu> Dear Lists: I got another telephone call at my lab yesterday from a solicitor. My phone number here is unlisted and does not show up on any caller id. I am getting 1 or 2 of these calls a week, too many to be random hits. I think some companies are harvesting my number from Listserv postings. Has anyone else had this experience? Geoff -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From mary.ann.deathridge <@t> Vanderbilt.Edu Thu May 24 11:16:19 2007 From: mary.ann.deathridge <@t> Vanderbilt.Edu (Deathridge, Mary Ann) Date: Thu May 24 11:16:26 2007 Subject: [Histonet] IHC cytology specimens Message-ID: <113162616B6440479A211712490222C684C3AE@mailbe10.mc.vanderbilt.edu> Does anyone in histoland have a protocol for the prep of cytology specimens (cytospins, smears) for IHC? Formalin fix? how long? air dried? 95% alc fix? esp. since the new Her2nu guidelines Thanks in advance Thanks MaryAnn Deathridge, HT (ASCP) Supervisor, Histopathology Vanderbilt Un. Med. Ctr Nashville, TN From rjbuesa <@t> yahoo.com Thu May 24 11:35:25 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu May 24 11:35:30 2007 Subject: [Histonet] IHC cytology specimens In-Reply-To: <113162616B6440479A211712490222C684C3AE@mailbe10.mc.vanderbilt.edu> Message-ID: <4099.48087.qm@web61214.mail.yahoo.com> If you use formalin will have to do HIER and the smears will not "survive" it. Use alcohol as fixative??air dry??PBS??IHC Ren?? J. "Deathridge, Mary Ann" wrote: Does anyone in histoland have a protocol for the prep of cytology specimens (cytospins, smears) for IHC? Formalin fix? how long? air dried? 95% alc fix? esp. since the new Her2nu guidelines Thanks in advance Thanks MaryAnn Deathridge, HT (ASCP) Supervisor, Histopathology Vanderbilt Un. Med. Ctr Nashville, TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Luggage? GPS? Comic books? Check out fitting gifts for grads at Yahoo! Search. From dlcowie <@t> prodigy.net Thu May 24 11:46:37 2007 From: dlcowie <@t> prodigy.net (Dawn Cowie) Date: Thu May 24 11:48:58 2007 Subject: [Histonet] Work hours In-Reply-To: <661949901A768E4F9CC16D8AF8F2838CA1C5E3@IS-E2K3.grhs.net> Message-ID: <713545.55935.qm@web81004.mail.mud.yahoo.com> We tried this at our lab. It worked really well for a while. We put together a schedule that allowed the tech to be off on Monday - gave them a 3 day weekend. The next week a tech would be off on Friday - gave them a 3 day weekend. The next week the tech would be off on Wednesday - they would then work Mon, Tues - be off Wed then work Thurs and Fri. Each tech was worked into the schedule this way. It was a bit difficult to work the details, but everyone really enjoyed it. We developed a problem tho when we lost 1 tech and 1 of the remaining techs needed to go back to the old schedule because of family issues. We no longer had enough techs to make it work so they all had to go back to the old schedule. My feeling is that as long as you have the work and enough techs to make it possible, its a good alternative to normal scheduling. Most of the techs don't feel like they're working a full time job. Just my 2 cents. Hope this gives you something useful. Dawn L. Cowie, HT (ASCP) Su Pensacola Pathologists, PA Pensacola, FL 32503 850-416-7251 Mike Pence wrote: How do you decide who gets which days off! Are they floating days? Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, May 23, 2007 11:52 AM To: Patti Loykasek; histonet Subject: Re: [Histonet] Work hours I have tried it. Cons: If yu don't have a flow of specimens in 4 days equivalent to the flow on 5 days, the personnel will be idling some time each day. If specimens or workload is available everybody will have work to do. Pros: it is fantastic having 3 days off weekly. Everybody likes that! Ren? J. Patti Loykasek wrote: I was wondering if anyone in histoland has experienced moving from a schedule of standard 8 hour day/5 days a week to a 10 hour day/4 days a week with techs having a day off during the week. If so, what are the pros and cons of this type of schedule. I'm interested in feedback from both bench techs and supervisor/managers. We are in the initial stages of contemplating this and would appreciate info from anyone that has tried it. Thank you. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> CDC.GOV Thu May 24 11:53:13 2007 From: jqb7 <@t> CDC.GOV (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Thu May 24 11:54:04 2007 Subject: [Histonet] Work hours In-Reply-To: <713545.55935.qm@web81004.mail.mud.yahoo.com> References: <661949901A768E4F9CC16D8AF8F2838CA1C5E3@IS-E2K3.grhs.net> <713545.55935.qm@web81004.mail.mud.yahoo.com> Message-ID: <34BB307EFC9A65429BBB49E330675F7201B0B72E@LTA3VS003.ees.hhs.gov> It's also a really nice way for the techs to save gas money and car wear. It's the "green" thing to do! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawn Cowie Sent: Thursday, May 24, 2007 12:47 PM To: Mike Pence; Rene J Buesa; Patti Loykasek; histonet Subject: RE: [Histonet] Work hours We tried this at our lab. It worked really well for a while. We put together a schedule that allowed the tech to be off on Monday - gave them a 3 day weekend. The next week a tech would be off on Friday - gave them a 3 day weekend. The next week the tech would be off on Wednesday - they would then work Mon, Tues - be off Wed then work Thurs and Fri. Each tech was worked into the schedule this way. It was a bit difficult to work the details, but everyone really enjoyed it. We developed a problem tho when we lost 1 tech and 1 of the remaining techs needed to go back to the old schedule because of family issues. We no longer had enough techs to make it work so they all had to go back to the old schedule. My feeling is that as long as you have the work and enough techs to make it possible, its a good alternative to normal scheduling. Most of the techs don't feel like they're working a full time job. Just my 2 cents. Hope this gives you something useful. Dawn L. Cowie, HT (ASCP) Su Pensacola Pathologists, PA Pensacola, FL 32503 850-416-7251 Mike Pence wrote: How do you decide who gets which days off! Are they floating days? Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, May 23, 2007 11:52 AM To: Patti Loykasek; histonet Subject: Re: [Histonet] Work hours I have tried it. Cons: If yu don't have a flow of specimens in 4 days equivalent to the flow on 5 days, the personnel will be idling some time each day. If specimens or workload is available everybody will have work to do. Pros: it is fantastic having 3 days off weekly. Everybody likes that! Ren? J. Patti Loykasek wrote: I was wondering if anyone in histoland has experienced moving from a schedule of standard 8 hour day/5 days a week to a 10 hour day/4 days a week with techs having a day off during the week. If so, what are the pros and cons of this type of schedule. I'm interested in feedback from both bench techs and supervisor/managers. We are in the initial stages of contemplating this and would appreciate info from anyone that has tried it. Thank you. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Thu May 24 12:01:32 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu May 24 12:01:24 2007 Subject: [Histonet] telephone solicitations In-Reply-To: <4655B30E.6030502@umdnj.edu> References: <4655B30E.6030502@umdnj.edu> Message-ID: <6.0.0.22.1.20070524104853.01b09530@gemini.msu.montana.edu> Geoff, Yes, and is the reason why I removed my phone and FAX numbers from signature for email postings. I have noticed some Histonetters do not put their phone numbers in signatures either. I also received unsolicited FAXs too. I tell them to not call back and not always very diplomatically. At 09:45 AM 5/24/2007, you wrote: >Dear Lists: > > I got another telephone call at my lab yesterday from a solicitor. My > phone number here is unlisted and does not show up on any caller id. I am > getting 1 or 2 of these calls a week, too many to be random hits. I think > some companies are harvesting my number from Listserv postings. Has > anyone else had this experience? Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From relia1 <@t> earthlink.net Thu May 24 12:30:53 2007 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu May 24 12:30:57 2007 Subject: [Histonet] Histo Tech Needed in Indianapolis Message-ID: Hi Histonetters I am currently working with a client in Indianapolis that is in need of an ASCP certified or eligible histo tech. This is a permanent full time dayshift position. The client offers a great lab environment, a great crew to work with and excellent benefits and salary. If you think you or someone you know might be interested in more detailed information about the opportunity, please contact me. I can be reached at 866-607-3542 or relia1@earthlink.net Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net From jennifer.l.hofecker <@t> Vanderbilt.Edu Thu May 24 13:01:47 2007 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Thu May 24 13:01:52 2007 Subject: [Histonet] Tennessee Society Hotel Deadline is Today Message-ID: <898D946569A27444B65667A49C074052D0E251@mailbe06.mc.vanderbilt.edu> Hello out there, Just wanted to remind everyone that the deadline for the hotel for the TSH meeting is today. After today, unused rooms will be released and may not be available. The meeting is June 7-9 in Dickson, Tn. If you haven't registered yet, it's not too late. We can register folks for classes up to and including the date of the meeting. The full meeting program may be accessed on the NSH website www.nsh.org under the calendar for region 3. The schedule at a glance is there with a place to download the complete program. Please email me if you have any questions or if we may be of any assistance to you. Thanks and enjoy the rest of the week, Jennifer Jennifer Hofecker HT(ASCP) Vanderbilt University Medical Center Neuropathology Laboratory Nashville TN phone: (615)343-0083 fax:(615)343-7089 TSH President NSH Quality Control Committee Chair From Shirley_PHUA <@t> hsa.gov.sg Thu May 24 13:14:40 2007 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Thu May 24 13:14:43 2007 Subject: [Histonet] Shirley is away 24 May 2007 afternoon ... Message-ID: I will be out of the office from 24-05-2007 to 25-05-2007. I will return on 25 May 2007. Pathologists : I will process your requests when I return. Otherwise, if urgent, please forward your mail to henry_kyaw@hsa.gov.sg Regrets for the inconveniences caused. From jhnspam <@t> aol.com Thu May 24 13:53:51 2007 From: jhnspam <@t> aol.com (pam johnson) Date: Thu May 24 13:54:06 2007 Subject: [Histonet] Job posting Message-ID: <8C96C4C01079F27-1920-17B3@WEBMAIL-RA14.sysops.aol.com> Job Title: Supervisor, ARC Histology (Job Number: 15541) Job Location: Memphis, TN??? ? St. Jude Children's Research Hospital, located in Memphis, Tennessee, is a premier center for research and treatment of potentially fatal childhood diseases, including cancer and certain blood, genetic, and immunodeficiency disorders. The hospital's mission is to advance cures, and means of prevention, for pediatric catastrophic diseases through research and treatment. St. Jude is dedicated to providing unsurpassed patient care and to advancing the health of children through biomedical research. ? Currently, St. Jude Children's Research Hospital has an opening for Supervisor- ARC Histology in the Animal Resources Center (Job Number 15541). ? This position supervises and effectively manages staff of the Veterinary Histology laboratory.? Supervises and performs laboratory procedures and the interpretation and reporting of results.? Assists with the evaluation and testing of new procedures and/or instrumentation; establishes maintenance protocols.? Ensures accurate records and quality control procedures are implemented and maintained as required by the hospital and accrediting, licensing/regulatory agencies (e.g. State of Tennessee, AAALAC, and USDA).? Also coordinates the laboratory budget. ? The successful candidate will have the following qualifications: A High school diploma or GED plus six (6) years of experience as a Histotechnologist or Histotechnician A Bachelor?s degree or equivalent (in concordance with current Federal and State Regulations) is preferred Veterinary histology experience is preferred Supervisory experience is also preferred Certification as a Histotechnologist or Histotechnician by the American Society of Clinical Pathology Board of Registry is required.? Subspecialty qualification in a related area (e.g. Immunohistochemistry) is preferred. ? ? St. Jude Children's Research Hospital is located in Memphis, a city rich in history and culture, with several outstanding theaters, museums, and nature preserves. Housing and other living expenses are very reasonable. ? Best Place to Work in Academia chosen by the readers of The Scientist. ? St. Jude Children's Research Hospital offers an excellent salary and benefits package. Qualified applicants may apply via our online process at www.stjude.org/jobs, referencing Job Number 15541. ? www.stjude.org An Equal Opportunity Employer __________________________________________________________________________________________________________________________ ? ? ? ? ? ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From jhnspam <@t> aol.com Thu May 24 13:58:32 2007 From: jhnspam <@t> aol.com (pam johnson) Date: Thu May 24 13:59:12 2007 Subject: [Histonet] Job posting Message-ID: <8C96C4CA8D481B1-1920-17EC@WEBMAIL-RA14.sysops.aol.com> Job Title: Supervisor, ARC Histology (Job Number: 15541) Job Location: Memphis, TN??? ? St. Jude Children's Research Hospital, located in Memphis, Tennessee, is a premier center for research and treatment of potentially fatal childhood diseases, including cancer and certain blood, genetic, and immunodeficiency disorders. The hospital's mission is to advance cures, and means of prevention, for pediatric catastrophic diseases through research and treatment. St. Jude is dedicated to providing unsurpassed patient care and to advancing the health of children through biomedical research. ? Currently, St. Jude Children's Research Hospital has an opening for Supervisor- ARC Histology in the Animal Resources Center (Job Number 15541). ? This position supervises and effectively manages staff of the Veterinary Histology laboratory.? Supervises and performs laboratory procedures and the interpretation and reporting of results.? Assists with the evaluation and testing of new procedures and/or instrumentation; establishes maintenance protocols.? Ensures accurate records and quality control procedures are implemented and maintained as required by the hospital and accrediting, licensing/regulatory agencies (e.g. State of Tennessee, AAALAC, and USDA).? Also coordinates the laboratory budget. ? The successful candidate will have the following qualifications: A High school diploma or GED plus six (6) years of experience as a Histotechnologist or Histotechnician A Bachelor?s degree or equivalent (in concordance with current Federal and State Regulations) is preferred Veterinary histology experience is preferred Supervisory experience is also preferred Certification as a Histotechnologist or Histotechnician by the American Society of Clinical Pathology Board of Registry is required.? Subspecialty qualification in a related area (e.g. Immunohistochemistry) is preferred. ? ? St. Jude Children's Research Hospital is located in Memphis, a city rich in history and culture, with several outstanding theaters, museums, and nature preserves. Housing and other living expenses are very reasonable. ? Best Place to Work in Academia chosen by the readers of The Scientist. ? St. Jude Children's Research Hospital offers an excellent salary and benefits package. Qualified applicants may apply via our online process at www.stjude.org/jobs, referencing Job Number 15541. ? www.stjude.org An Equal Opportunity Employer __________________________________________________________________________________________________________________________ ? ? ? ? ? ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From Tamara.Franz-Odendaal <@t> msvu.ca Thu May 24 15:35:19 2007 From: Tamara.Franz-Odendaal <@t> msvu.ca (Tamara Franz-Odendaal) Date: Thu May 24 15:35:38 2007 Subject: [Histonet] Nikon NIS-Elements - digital camera software problem; software others use?? Message-ID: HI you may want to check the specifications of your computer. I am about to purchase hte system and have been told that some microchip data sets in some PCs conflict with the NIS elements software - they will only guarantee the software working with some PCs - i.e. some pentium 4's and not all of them. I suggest you call the imaging experts at Nikon to discuss. Tamara Tamara Franz-Odendaal (PhD) Assistant Professor of Biology, Mount Saint Vincent University 166 Bedford Highway Halifax, NS, B3M 2J6 Canada Tel: +1 902 - 457 6140 Fax: +1 902 - 457 6455 http://faculty.msvu.ca/tfodendaal/ >>> "Connolly, Brett M" 05/24/07 12:18 PM >>> Gustave, I use NIS-Elements and have not experienced any of those problems. Ours was installed earlier this year. Brett M. Connolly, Ph.D. Research Fellow MRL, Imaging Research Merck & Co., Inc. WP-44K PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of GT Hebert Sent: Thursday, May 24, 2007 11:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Nikon NIS-Elements - digital camera software problem; software others use?? Hello, Is anyone out there using a Nikon camera with NIS-elements (Advanced Research) software for digital imaging capture and digital image analysis?? Since buying the microscope system, I have been experiencing a multitude of software problems. In particular, during image 'capture', the computer will crash - a blue screen with white script would pop up and the computer subsequently will restart itself. Upon trying to open the software again, an error message would come up. I would have to restart the computer manually and open the software again. Nikon has come up with higher versions of the software and the updates have been installed, but as of yet, the problem has not been fixed. So my questions are as follows: Do you currently us NIS-Elements? If so, have you experienced similar issues? What image capturing software is the BEST both in terms of analysis and image capture? If I have to, I will eventually move on to a better system if this one continues to give me problems. Your answers and insight is much appreciated. Thank you in advance. Gustave Hebert Scientist II Wyeth Research Cambridge MA --------------------------------- 8:00? 8:25? 8:40? Find a flick in no time with theYahoo! Search movie showtime shortcut. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication, including any attached documentation, is intended only for the person or entity to which it is addressed, and may contain confidential, personal, and/or privileged information. Any unauthorized disclosure, copying, or taking action on the contents is strictly prohibited. If you have received this message in error, please contact us immediately so we may correct our records. Please then delete or destroy the original transmission and any subsequent reply. Thank you. From carl.hobbs <@t> kcl.ac.uk Thu May 24 15:52:50 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Thu May 24 15:53:15 2007 Subject: [Histonet] Abcam survey Message-ID: <006e01c79e45$7dc8c470$4101a8c0@carlba65530bda> They are interested in finding out how many of us Immunogeeks need Abs specifically directed against animal proteins. I have never encountered such interest before. Sure , many Abs are x-reactive: however, I have spent loads of money testing Abs out, that are human-reactive but their x-species reactivity is not known. ( I work with non-human sections, mostly) Many are useless in non-human pwax tissues. I am a researcher and have no commercial "profile" ( other than accumulating Abcam points, lol) Please visit this site http://www.immunoportal.com/index.php and add any comments. Thanks Carlos From carl.hobbs <@t> kcl.ac.uk Thu May 24 15:52:50 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Thu May 24 15:53:20 2007 Subject: [Histonet] Abcam survey Message-ID: <006f01c79e45$82c1f5f0$4101a8c0@carlba65530bda> They are interested in finding out how many of us Immunogeeks need Abs specifically directed against animal proteins. I have never encountered such interest before. Sure , many Abs are x-reactive: however, I have spent loads of money testing Abs out, that are human-reactive but their x-species reactivity is not known. ( I work with non-human sections, mostly) Many are useless in non-human pwax tissues. I am a researcher and have no commercial "profile" ( other than accumulating Abcam points, lol) Please visit this site http://www.immunoportal.com/index.php and add any comments. Thanks Carlos From tennyjin <@t> gmail.com Thu May 24 16:18:36 2007 From: tennyjin <@t> gmail.com (tenny jin) Date: Thu May 24 16:18:42 2007 Subject: [Histonet] Mouse specific M2/M6 antibody staining protocol Message-ID: Dear all, Has anyone had some hand-on experience with mouse specific M2/M6 antibody (from Iowa Hybridoma bank) to detect mouse cells transplanted in the rat brain (cryosection)? I have trouble with the high background staining. If someone could share the staining protocol, I will appreciate it. Thanks in advance! Tenny From c.m.vanderloos <@t> amc.uva.nl Fri May 25 03:22:32 2007 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Fri May 25 03:22:49 2007 Subject: [Histonet] RE: IHC cytology specimens Message-ID: <67bc0c67ba84.67ba8467bc0c@amc.uva.nl> Mary Ann, I am answering this from a research point of view, totally unaware of Her2neu guidelines or what so ever. We fixed cytospins in NBF (5 min, RT), washed them with tap water (5 min) and then to alcohol 70 (3 hours - overnight). Wash with tap water and perform HIER with appropriate buffer. We use half the time at max. temperature as for FFPE's. After cooling down, wash with tap water and perform IHC as with FFPE tissue sections (antibody, dilution, detection). I am aware this procedure as against all logics but it simple works. After all, 5 min NBF fixation cannot cause any cross linking and alcohol fixation certainly not. Therefore, HIER would not be necessary. But after testing we saw HIER was very essential! When I heard about this procedure I was very sceptical it would ever work. However, I was surprised to see how well the cells survive this harsh procedure and nicely stay at the glass. For some particular antigens we have even done this procedure successfully with cryostat tissue sections from fresh frozen blocks (section stays at the glass, morphology is fine)! Therefore folks: a theory is good to keep in mind, but testing is sometimes better. And, I also stopped with surprising myself about "strange" protocols. I hope this helps. Have a nice weekend, Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Thu, 24 May 2007 11:16:19 -0500 From: "Deathridge, Mary Ann" Subject: [Histonet] IHC cytology specimens To: Does anyone in histoland have a protocol for the prep of cytology specimens (cytospins, smears) for IHC? Formalin fix? how long? air dried? 95% alc fix? esp. since the new Her2nu guidelines Thanks in advance Thanks MaryAnn Deathridge, HT (ASCP) Supervisor, Histopathology Vanderbilt Un. Med. Ctr Nashville, TN From ian.montgomery <@t> bio.gla.ac.uk Fri May 25 05:53:56 2007 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Fri May 25 05:54:06 2007 Subject: [Histonet] Neurogenesis Message-ID: <001501c79eba$fe917640$4724d182@ibls.gla.ac.uk> I'm just about to start my literature search for information on a new neurogenesis project. In the first instance I'll only be looking at markers for new neurones so any hints and tips pointing me in the right direction would be welcome. Recently a topic has covered freezing tissue in iso-pentane cooled with solid CO2. This is also of interest for another project where I'll be studying skeletal muscle from salmon. My routine technique is iso-pentane cooled using liquid nitrogen and for an earlier pilot study this was the technique used. But, these salmon specimens are taken from pens in Scottish sea lochs so technically it's a wee bit tricky for the scientists freezing the specimens. Remember, this is Scotland where wind, rain and heaving seas are normal so using liquid nitrogen presents health and safety issues. A question for those who have frozen muscle using CO2 cooled iso-pentane? Can it be done successfully and repeatedly without ice-crystal damage? Obviously I'll get the people at the fish farm to try it before the experiments start but I don't want them to waste their time as in the commercial setting, time is money. I should also point out that Scottish salmon is like the whisky, the gift of the gods. Ian. Dr. Ian Montgomery, Histotechnology, IBLS Support Services, Thomson Building, University of Glasgow, Tel:01413398855 Extn: 8511. From matt.prideaux <@t> bbsrc.ac.uk Fri May 25 06:09:11 2007 From: matt.prideaux <@t> bbsrc.ac.uk (matt prideaux (RI)) Date: Fri May 25 06:09:35 2007 Subject: [Histonet] DAPI staining methacrylate sections Message-ID: <84DA9D8AC9B05F4B889E7C70238CB45104C52B7E@rie2ksrv1.ri.bbsrc.ac.uk> Hi everyone, I'm trying to find a method to examine osteocyte density in areas of new bone formation (between two calcein labels). The sections that we have are 200 micron thick sections of mouse tibia. We have a confocal microscope, which gives great images of the labels but the osteocytes are not easily seen. I have heard that DAPI would be an ideal stain for the osteocyte nuclei, but is it possible to stain methacrylate embedded sections with DAPI and if so does anyone have a method for this? Thanks, Matt Matt Prideaux Bone Biology Group Gene Function and Development Roslin Institute Roslin Midlothian EH25 9PS Tel: +44 131 5274 244/254 Institute Fax: +44 131 440 0434 Roslin Institute is a company limited by guarantee, registered in Scotland (registered number SC157100) and a Scottish Charity (registered number SC023592). Our registered office is at Roslin, Midlothian, EH25 9PS. VAT registration number 847380013. The information contained in this e-mail (including any attachments) is confidential and is intended for the use of the addressee only. The opinions expressed within this e-mail (including any attachments) are the opinions of the sender and do not necessarily constitute those of Roslin Institute (Edinburgh) ("the Institute") unless specifically stated by a sender who is duly authorised to do so on behalf of the Institute From MAUGER <@t> email.chop.edu Fri May 25 07:37:24 2007 From: MAUGER <@t> email.chop.edu (Joanne Mauger) Date: Fri May 25 07:38:08 2007 Subject: [Histonet] RE: IHC cytology specimens Message-ID: Hi, I agree with Chris. NBF actually seems to help tissue stay on slide better. I will post fix a smear or cytospin in NBF for 15 mins. that has been previously fixed in alcohol. I do HIER as I would on FFPE. I have found that some antibodies will not show expression unless this post NBF fixation and retrieval has been done-eg. INI-1. I have also found retrieval necessary on thin prep cytospins that had been only fixed in sacamano,and 95% etoh. Trial and error is the key. Jo From twheelock <@t> mclean.harvard.edu Fri May 25 08:36:58 2007 From: twheelock <@t> mclean.harvard.edu (Tim Wheelock) Date: Fri May 25 08:07:33 2007 Subject: [Histonet] Custom made solutions Message-ID: <4656E67A.4040309@mclean.harvard.edu> Hi Everyone: Does anyone know of a company who makes custom-made solutions? I am finding it too time consuming and distracting to regularly make a cryoprotectant that has 5 ingredients and requires heat. Thanks, Tim Wheelock Harvard Brain Brain Belmont, MA P.S. The cryoprotectant requires: polyvinylpyrrolidone (PVP-40) sucrose sodium chloride sodium phosphate monobasic monohydrate sodium phosphate dibasic heptahydrate ethylene glycol and water. Any information, including protected health information (PHI), transmitted in this email is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential and/or exempt from disclosure under applicable Federal or State law. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon, PHI by persons or entities other than the intended recipient is prohibited. If you received this email in error, please contact the sender and delete the material from any computer. From cgfields <@t> lexhealth.org Fri May 25 08:19:33 2007 From: cgfields <@t> lexhealth.org (Carole Fields) Date: Fri May 25 08:19:37 2007 Subject: [Histonet] Custom made solutions In-Reply-To: <4656E67A.4040309@mclean.harvard.edu> Message-ID: I ran into the same situation and contacted Poly Scientific and they made up my solution and packaged it in prefilled containers. Also BBC will do the same....I learned after the fact. Either place is great and accommodating. Let me know if you are interested and I can give you the details. Carole Fields, HT,ASCP Pathology Supervisor Lexington Medical Center 2720 Sunset Blvd. W. Columbia, SC 29169 -----Original Message----- From: Tim Wheelock [mailto:twheelock@mclean.harvard.edu] Sent: Friday, May 25, 2007 9:37 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Custom made solutions Hi Everyone: Does anyone know of a company who makes custom-made solutions? I am finding it too time consuming and distracting to regularly make a cryoprotectant that has 5 ingredients and requires heat. Thanks, Tim Wheelock Harvard Brain Brain Belmont, MA P.S. The cryoprotectant requires: polyvinylpyrrolidone (PVP-40) sucrose sodium chloride sodium phosphate monobasic monohydrate sodium phosphate dibasic heptahydrate ethylene glycol and water. Any information, including protected health information (PHI), transmitted in this email is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential and/or exempt from disclosure under applicable Federal or State law. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon, PHI by persons or entities other than the intended recipient is prohibited. If you received this email in error, please contact the sender and delete the material from any computer. From Vickroy.Jim <@t> mhsil.com Fri May 25 09:14:13 2007 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Fri May 25 09:14:21 2007 Subject: [Histonet] test slides for detection kits Message-ID: How long are we required to keep test slides for documenting detection kits? Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From abright <@t> brightinstruments.com Fri May 25 09:29:54 2007 From: abright <@t> brightinstruments.com (Alan Bright) Date: Fri May 25 09:26:25 2007 Subject: [Histonet] Neurogenesis References: <001501c79eba$fe917640$4724d182@ibls.gla.ac.uk> Message-ID: Dear Ian, On the subject of rapid freezing, I would like to make you aware that we manufacture the Bright Clini-RF Ultra low rapid freezer which is being used as a replacement for CO2 & liquid nitrogen. At present we have one type that's rapid freezing tank operates at -80 degs C and will shortly have another model for -100 degs C. There are also options available for controlling the temperature too. Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com Skype: dazzle0 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian Montgomery Sent: 25 May 2007 11:54 To: Histonet Subject: [Histonet] Neurogenesis I'm just about to start my literature search for information on a new neurogenesis project. In the first instance I'll only be looking at markers for new neurones so any hints and tips pointing me in the right direction would be welcome. Recently a topic has covered freezing tissue in iso-pentane cooled with solid CO2. This is also of interest for another project where I'll be studying skeletal muscle from salmon. My routine technique is iso-pentane cooled using liquid nitrogen and for an earlier pilot study this was the technique used. But, these salmon specimens are taken from pens in Scottish sea lochs so technically it's a wee bit tricky for the scientists freezing the specimens. Remember, this is Scotland where wind, rain and heaving seas are normal so using liquid nitrogen presents health and safety issues. A question for those who have frozen muscle using CO2 cooled iso-pentane? Can it be done successfully and repeatedly without ice-crystal damage? Obviously I'll get the people at the fish farm to try it before the experiments start but I don't want them to waste their time as in the commercial setting, time is money. I should also point out that Scottish salmon is like the whisky, the gift of the gods. Ian. Dr. Ian Montgomery, Histotechnology, IBLS Support Services, Thomson Building, University of Glasgow, Tel:01413398855 Extn: 8511. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Fri May 25 10:09:31 2007 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri May 25 10:09:37 2007 Subject: [Histonet] Stainer Comparison Message-ID: <4D14F0FC9316DD41972D5F03C070908B8F449E@nmdamailsvr.nmda.ad.nmsu.edu> I have the opportunity to purchase an automatic stainer. I'm comparing the Leica Autostainer XL and the Sakura Tissue-Tek Prisma. If you had your 'druthers, which would you choose? Would you please reply OFF LIST with your pros and cons? Thank you! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 From sbreeden <@t> nmda.nmsu.edu Fri May 25 10:11:39 2007 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri May 25 10:11:45 2007 Subject: [Histonet] Donation of Slides? Message-ID: <4D14F0FC9316DD41972D5F03C070908B8F449F@nmdamailsvr.nmda.ad.nmsu.edu> Our local community college has a very popular vet(erinary) tech program; part of their training is making blood smears/touch preps/etc. They now wash and reuse slides several times and are in need of a donation of new slides. Is there a vendor out there that could donate a quantity of frosted, basic slides to this program? Please contact me directly if you would be willing to help out. Thanks very much! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 From rjbuesa <@t> yahoo.com Fri May 25 10:48:43 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri May 25 10:48:48 2007 Subject: [Histonet] Stainer Comparison In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B8F449E@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <574862.65239.qm@web61214.mail.yahoo.com> Sakura Tissue-Tek Prisma! Ren? J. "Breeden, Sara" wrote: I have the opportunity to purchase an automatic stainer. I'm comparing the Leica Autostainer XL and the Sakura Tissue-Tek Prisma. If you had your 'druthers, which would you choose? Would you please reply OFF LIST with your pros and cons? Thank you! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Don't pick lemons. See all the new 2007 cars at Yahoo! Autos. From mpence <@t> grhs.net Fri May 25 11:08:24 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Fri May 25 11:08:38 2007 Subject: [Histonet] FW: Ventana Users Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C5E8@IS-E2K3.grhs.net> -----Original Message----- From: Mike Pence Sent: Friday, May 25, 2007 10:03 AM To: histonet-bounces@lists.utsouthwestern.edu Subject: Ventana Users I was wondering how many of you out there are using the Ventana Automated IHC System. I would like to build a network of like users to have as a resource when developing protocols in my IHC lab. We currently offer about 30 standard antibodies and I work up another 1 or 2 a month. It seems that when I call Ventana for protocol assistance they most times head me in the wrong direction. Thanks in advance for sharing your e-mail or thoughts with me. Mike From liz <@t> premierlab.com Fri May 25 11:35:32 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri May 25 11:35:38 2007 Subject: [Histonet] help with some references Message-ID: <000001c79eea$b6e3b260$0d00a8c0@domain.Premier> Hello all I was wondering if there was anyone out there that had copies of two articles that they could fax or e-mail to me. One is from the Journal of Histotechnology from 1990, I have already requested this through the NSH web page but was hoping I could get it sooner. Its on species IHC cross reactivity and I think that Roberta Smith wrote it, but all I have is the following: Smith RA. J Histotechnology 1990;13(4): 255-69 And the other is from Applied Immunohistochemistry and Molecular Morphology also on antibody species cross reactivity Martin CA and Badran AF, Applied Immunohistochem 1998;6(2) :84-8 I don't have titles Thanks in advance and to all out here have a great Memorial Day weekend! Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 From dmccaig <@t> ckha.on.ca Fri May 25 11:58:18 2007 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Fri May 25 11:58:23 2007 Subject: [Histonet] gordon and sweetsd retic stain Message-ID: Hi I have done this stain successfuly for many years and the procedure has never changed. Yesterday, it failed to stain any retic and the repeat with all reagents prepared fresh gave the same results. Has anyone got any suggestions where I can start to investigate this? Diana From mrsseagle <@t> yahoo.com Fri May 25 12:01:44 2007 From: mrsseagle <@t> yahoo.com (MICHELLE SEAGLE) Date: Fri May 25 12:01:52 2007 Subject: [Histonet] (no subject) Message-ID: <785967.6737.qm@web51812.mail.re2.yahoo.com> Hi everyone. Our histo lab is trying to gather some suggestions on a few pieces of equipment we are needing for our lab in the next budget year. Your suggestions would be greatly appreciated. Let us know what you like and dont like out there!!! EQUIPMENT NEEDED: TISSUE PROCESSOR CRYOSTAT EMBEDDING CENTER H&E STAINER MICROSCOPE PARAFFIN DISPENSER CASSETTE LABELER/SLIDE LABELER SLIDE DRYER CYTOSPIN Thanks Michelle Seagle HT (ASCP) Rutherford Hospital --------------------------------- Park yourself in front of a world of choices in alternative vehicles. Visit the Yahoo! Auto Green Center. From rjbuesa <@t> yahoo.com Fri May 25 12:05:29 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri May 25 12:05:33 2007 Subject: [Histonet] (no subject) In-Reply-To: <785967.6737.qm@web51812.mail.re2.yahoo.com> Message-ID: <358256.78122.qm@web61211.mail.yahoo.com> For the microscope go with Leica. Try to get a catalog from Sakura-Finetek for the others, except for cytospin, slide dryer and paraffin dispenser. Ren? J. MICHELLE SEAGLE wrote: Hi everyone. Our histo lab is trying to gather some suggestions on a few pieces of equipment we are needing for our lab in the next budget year. Your suggestions would be greatly appreciated. Let us know what you like and dont like out there!!! EQUIPMENT NEEDED: TISSUE PROCESSOR CRYOSTAT EMBEDDING CENTER H&E STAINER MICROSCOPE PARAFFIN DISPENSER CASSETTE LABELER/SLIDE LABELER SLIDE DRYER CYTOSPIN Thanks Michelle Seagle HT (ASCP) Rutherford Hospital --------------------------------- Park yourself in front of a world of choices in alternative vehicles. Visit the Yahoo! Auto Green Center. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Bored stiff? Loosen up... Download and play hundreds of games for free on Yahoo! Games. From mpence <@t> grhs.net Fri May 25 12:24:14 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Fri May 25 12:24:19 2007 Subject: [Histonet] (no subject) In-Reply-To: <358256.78122.qm@web61211.mail.yahoo.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C5EA@IS-E2K3.grhs.net> Leica also makes many of the other items on your list. Sakura-Finetek and Thermo/Shandon/Fischer/Lipshaw (not sure what their called today) have those items as well. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, May 25, 2007 12:05 PM To: MICHELLE SEAGLE; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] (no subject) For the microscope go with Leica. Try to get a catalog from Sakura-Finetek for the others, except for cytospin, slide dryer and paraffin dispenser. Ren? J. MICHELLE SEAGLE wrote: Hi everyone. Our histo lab is trying to gather some suggestions on a few pieces of equipment we are needing for our lab in the next budget year. Your suggestions would be greatly appreciated. Let us know what you like and dont like out there!!! EQUIPMENT NEEDED: TISSUE PROCESSOR CRYOSTAT EMBEDDING CENTER H&E STAINER MICROSCOPE PARAFFIN DISPENSER CASSETTE LABELER/SLIDE LABELER SLIDE DRYER CYTOSPIN Thanks Michelle Seagle HT (ASCP) Rutherford Hospital --------------------------------- Park yourself in front of a world of choices in alternative vehicles. Visit the Yahoo! Auto Green Center. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Bored stiff? Loosen up... Download and play hundreds of games for free on Yahoo! Games. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Herrick.James <@t> mayo.edu Fri May 25 12:35:52 2007 From: Herrick.James <@t> mayo.edu (Herrick, James L.) Date: Fri May 25 12:35:57 2007 Subject: [Histonet] Plastic Embedding Message-ID: <572057D3BDD52A46BD05BC6DA50686110CAE5E@MSGEBE22.mfad.mfroot.org> Hello everyone, Does anyone have any experience with plastic embedding using Glycol Methacrylate (GMA), Methyl Methacrylate (MMA) or other products that could be used to embed vertebral specimens large enough that require approximately 100 mLs of solution to embed completely. The problem I am having using GMA is that the reaction becomes extremely hot and polymerizes into a block full of holes and bubbles. I would really appreciate any advice, protocols, etc., that you may have. Thanks. Jim From catherine.scott <@t> mpiresearch.com Fri May 25 12:51:54 2007 From: catherine.scott <@t> mpiresearch.com (Catherine Scott) Date: Fri May 25 12:51:59 2007 Subject: [Histonet] DLM Certification Message-ID: Hello, I am looking for any insight on this exam. Apparently, there are no pre-test exams available for this certification. I would greatly appreciate it if anyone can share their experience with me that has taken the exam or if you may have more information then what I have been able to locate on the ASCP web page. Thank you, Cathy Catherine L. Scott, BA, H.T.(ASCP), A.L.A.T Associate Director, Laboratory Services 54943 North Main Street Mattawan, Michigan 49071-9399 USA Telephone: 269.668.3336 ext. 1218 Fax: 269.668.4151 E-mail: catherine.scott@mpiresearch.com Website: www.mpiresearch.com From AFeatherstone <@t> KaleidaHealth.Org Fri May 25 13:13:13 2007 From: AFeatherstone <@t> KaleidaHealth.Org (Featherstone, Annette) Date: Fri May 25 13:13:23 2007 Subject: [Histonet] Private autopsy pricing In-Reply-To: References: Message-ID: Does anyone have pricing for private autopsy, complete, partial, brain only, etc? Annette Featherstone Kaleida Health -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, May 25, 2007 12:57 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 42, Issue 37 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: telephone solicitations (Gayle Callis) 2. Histo Tech Needed in Indianapolis (Pam Barker) 3. Tennessee Society Hotel Deadline is Today (Hofecker, Jennifer L) 4. Shirley is away 24 May 2007 afternoon ... (Shirley PHUA) 5. Job posting (pam johnson) 6. Job posting (pam johnson) 7. RE: Nikon NIS-Elements - digital camera software problem; software others use?? (Tamara Franz-Odendaal) 8. Abcam survey (Carl Hobbs) 9. Abcam survey (Carl Hobbs) 10. Mouse specific M2/M6 antibody staining protocol (tenny jin) 11. RE: IHC cytology specimens (C.M. van der Loos) 12. Neurogenesis (Ian Montgomery) 13. DAPI staining methacrylate sections (matt prideaux (RI)) 14. RE: IHC cytology specimens (Joanne Mauger) 15. Custom made solutions (Tim Wheelock) 16. RE: Custom made solutions (Carole Fields) 17. test slides for detection kits (Vickroy, Jim) 18. RE: Neurogenesis (Alan Bright) 19. Stainer Comparison (Breeden, Sara) 20. Donation of Slides? (Breeden, Sara) 21. Re: Stainer Comparison (Rene J Buesa) 22. FW: Ventana Users (Mike Pence) 23. help with some references (Liz Chlipala) 24. gordon and sweetsd retic stain (Diana McCaig) ---------------------------------------------------------------------- Message: 1 Date: Thu, 24 May 2007 11:01:32 -0600 From: Gayle Callis Subject: Re: [Histonet] telephone solicitations To: Geoff McAuliffe , Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20070524104853.01b09530@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Geoff, Yes, and is the reason why I removed my phone and FAX numbers from signature for email postings. I have noticed some Histonetters do not put their phone numbers in signatures either. I also received unsolicited FAXs too. I tell them to not call back and not always very diplomatically. At 09:45 AM 5/24/2007, you wrote: >Dear Lists: > > I got another telephone call at my lab yesterday from a solicitor. > My phone number here is unlisted and does not show up on any caller > id. I am getting 1 or 2 of these calls a week, too many to be random > hits. I think some companies are harvesting my number from Listserv > postings. Has anyone else had this experience? Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 ------------------------------ Message: 2 Date: Thu, 24 May 2007 13:30:53 -0400 From: "Pam Barker" Subject: [Histonet] Histo Tech Needed in Indianapolis To: "'Histonet'" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Histonetters I am currently working with a client in Indianapolis that is in need of an ASCP certified or eligible histo tech. This is a permanent full time dayshift position. The client offers a great lab environment, a great crew to work with and excellent benefits and salary. If you think you or someone you know might be interested in more detailed information about the opportunity, please contact me. I can be reached at 866-607-3542 or relia1@earthlink.net Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net ------------------------------ Message: 3 Date: Thu, 24 May 2007 13:01:47 -0500 From: "Hofecker, Jennifer L" Subject: [Histonet] Tennessee Society Hotel Deadline is Today To: Message-ID: <898D946569A27444B65667A49C074052D0E251@mailbe06.mc.vanderbilt.edu> Content-Type: text/plain; charset="iso-8859-1" Hello out there, Just wanted to remind everyone that the deadline for the hotel for the TSH meeting is today. After today, unused rooms will be released and may not be available. The meeting is June 7-9 in Dickson, Tn. If you haven't registered yet, it's not too late. We can register folks for classes up to and including the date of the meeting. The full meeting program may be accessed on the NSH website www.nsh.org under the calendar for region 3. The schedule at a glance is there with a place to download the complete program. Please email me if you have any questions or if we may be of any assistance to you. Thanks and enjoy the rest of the week, Jennifer Jennifer Hofecker HT(ASCP) Vanderbilt University Medical Center Neuropathology Laboratory Nashville TN phone: (615)343-0083 fax:(615)343-7089 TSH President NSH Quality Control Committee Chair ------------------------------ Message: 4 Date: Fri, 25 May 2007 02:14:40 +0800 From: Shirley PHUA Subject: [Histonet] Shirley is away 24 May 2007 afternoon ... To: histonet Message-ID: Content-Type: text/plain; charset=US-ASCII I will be out of the office from 24-05-2007 to 25-05-2007. I will return on 25 May 2007. Pathologists : I will process your requests when I return. Otherwise, if urgent, please forward your mail to henry_kyaw@hsa.gov.sg Regrets for the inconveniences caused. ------------------------------ Message: 5 Date: Thu, 24 May 2007 14:53:51 -0400 From: pam johnson Subject: [Histonet] Job posting To: histonet@lists.utsouthwestern.edu Message-ID: <8C96C4C01079F27-1920-17B3@WEBMAIL-RA14.sysops.aol.com> Content-Type: text/plain; charset="utf-8" Job Title: Supervisor, ARC Histology (Job Number: 15541) Job Location: Memphis, TN?????? ?? St. Jude Children's Research Hospital, located in Memphis, Tennessee, is a premier center for research and treatment of potentially fatal childhood diseases, including cancer and certain blood, genetic, and immunodeficiency disorders. The hospital's mission is to advance cures, and means of prevention, for pediatric catastrophic diseases through research and treatment. St. Jude is dedicated to providing unsurpassed patient care and to advancing the health of children through biomedical research. ?? Currently, St. Jude Children's Research Hospital has an opening for Supervisor- ARC Histology in the Animal Resources Center (Job Number 15541). ?? This position supervises and effectively manages staff of the Veterinary Histology laboratory.?? Supervises and performs laboratory procedures and the interpretation and reporting of results.?? Assists with the evaluation and testing of new procedures and/or instrumentation; establishes maintenance protocols.?? Ensures accurate records and quality control procedures are implemented and maintained as required by the hospital and accrediting, licensing/regulatory agencies (e.g. State of Tennessee, AAALAC, and USDA).?? Also coordinates the laboratory budget. ?? The successful candidate will have the following qualifications: A High school diploma or GED plus six (6) years of experience as a Histotechnologist or Histotechnician A Bachelor???s degree or equivalent (in concordance with current Federal and State Regulations) is preferred Veterinary histology experience is preferred Supervisory experience is also preferred Certification as a Histotechnologist or Histotechnician by the American Society of Clinical Pathology Board of Registry is required.?? Subspecialty qualification in a related area (e.g. Immunohistochemistry) is preferred. ?? ?? St. Jude Children's Research Hospital is located in Memphis, a city rich in history and culture, with several outstanding theaters, museums, and nature preserves. Housing and other living expenses are very reasonable. ?? Best Place to Work in Academia chosen by the readers of The Scientist. ?? St. Jude Children's Research Hospital offers an excellent salary and benefits package. Qualified applicants may apply via our online process at www.stjude.org/jobs, referencing Job Number 15541. ?? www.stjude.org An Equal Opportunity Employer __________________________________________________________________________________________________________________________ ?? ?? ?? ?? ?? ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. ------------------------------ Message: 6 Date: Thu, 24 May 2007 14:58:32 -0400 From: pam johnson Subject: [Histonet] Job posting To: histonet@lists.utsouthwestern.edu Message-ID: <8C96C4CA8D481B1-1920-17EC@WEBMAIL-RA14.sysops.aol.com> Content-Type: text/plain; charset="utf-8" Job Title: Supervisor, ARC Histology (Job Number: 15541) Job Location: Memphis, TN?????? ?? St. Jude Children's Research Hospital, located in Memphis, Tennessee, is a premier center for research and treatment of potentially fatal childhood diseases, including cancer and certain blood, genetic, and immunodeficiency disorders. The hospital's mission is to advance cures, and means of prevention, for pediatric catastrophic diseases through research and treatment. St. Jude is dedicated to providing unsurpassed patient care and to advancing the health of children through biomedical research. ?? Currently, St. Jude Children's Research Hospital has an opening for Supervisor- ARC Histology in the Animal Resources Center (Job Number 15541). ?? This position supervises and effectively manages staff of the Veterinary Histology laboratory.?? Supervises and performs laboratory procedures and the interpretation and reporting of results.?? Assists with the evaluation and testing of new procedures and/or instrumentation; establishes maintenance protocols.?? Ensures accurate records and quality control procedures are implemented and maintained as required by the hospital and accrediting, licensing/regulatory agencies (e.g. State of Tennessee, AAALAC, and USDA).?? Also coordinates the laboratory budget. ?? The successful candidate will have the following qualifications: A High school diploma or GED plus six (6) years of experience as a Histotechnologist or Histotechnician A Bachelor???s degree or equivalent (in concordance with current Federal and State Regulations) is preferred Veterinary histology experience is preferred Supervisory experience is also preferred Certification as a Histotechnologist or Histotechnician by the American Society of Clinical Pathology Board of Registry is required.?? Subspecialty qualification in a related area (e.g. Immunohistochemistry) is preferred. ?? ?? St. Jude Children's Research Hospital is located in Memphis, a city rich in history and culture, with several outstanding theaters, museums, and nature preserves. Housing and other living expenses are very reasonable. ?? Best Place to Work in Academia chosen by the readers of The Scientist. ?? St. Jude Children's Research Hospital offers an excellent salary and benefits package. Qualified applicants may apply via our online process at www.stjude.org/jobs, referencing Job Number 15541. ?? www.stjude.org An Equal Opportunity Employer __________________________________________________________________________________________________________________________ ?? ?? ?? ?? ?? ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. ------------------------------ Message: 7 Date: Thu, 24 May 2007 17:35:19 -0300 From: "Tamara Franz-Odendaal" Subject: RE: [Histonet] Nikon NIS-Elements - digital camera software problem; software others use?? To: Message-ID: Content-Type: text/plain; charset=US-ASCII HI you may want to check the specifications of your computer. I am about to purchase hte system and have been told that some microchip data sets in some PCs conflict with the NIS elements software - they will only guarantee the software working with some PCs - i.e. some pentium 4's and not all of them. I suggest you call the imaging experts at Nikon to discuss. Tamara Tamara Franz-Odendaal (PhD) Assistant Professor of Biology, Mount Saint Vincent University 166 Bedford Highway Halifax, NS, B3M 2J6 Canada Tel: +1 902 - 457 6140 Fax: +1 902 - 457 6455 http://faculty.msvu.ca/tfodendaal/ >>> "Connolly, Brett M" 05/24/07 12:18 PM >>> Gustave, I use NIS-Elements and have not experienced any of those problems. Ours was installed earlier this year. Brett M. Connolly, Ph.D. Research Fellow MRL, Imaging Research Merck & Co., Inc. WP-44K PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of GT Hebert Sent: Thursday, May 24, 2007 11:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Nikon NIS-Elements - digital camera software problem; software others use?? Hello, Is anyone out there using a Nikon camera with NIS-elements (Advanced Research) software for digital imaging capture and digital image analysis?? Since buying the microscope system, I have been experiencing a multitude of software problems. In particular, during image 'capture', the computer will crash - a blue screen with white script would pop up and the computer subsequently will restart itself. Upon trying to open the software again, an error message would come up. I would have to restart the computer manually and open the software again. Nikon has come up with higher versions of the software and the updates have been installed, but as of yet, the problem has not been fixed. So my questions are as follows: Do you currently us NIS-Elements? If so, have you experienced similar issues? What image capturing software is the BEST both in terms of analysis and image capture? If I have to, I will eventually move on to a better system if this one continues to give me problems. Your answers and insight is much appreciated. Thank you in advance. Gustave Hebert Scientist II Wyeth Research Cambridge MA --------------------------------- 8:00? 8:25? 8:40? Find a flick in no time with theYahoo! Search movie showtime shortcut. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. 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Thank you. ------------------------------ Message: 8 Date: Thu, 24 May 2007 21:52:50 +0100 From: "Carl Hobbs" Subject: [Histonet] Abcam survey To: "Histonet" Message-ID: <006e01c79e45$7dc8c470$4101a8c0@carlba65530bda> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original They are interested in finding out how many of us Immunogeeks need Abs specifically directed against animal proteins. I have never encountered such interest before. Sure , many Abs are x-reactive: however, I have spent loads of money testing Abs out, that are human-reactive but their x-species reactivity is not known. ( I work with non-human sections, mostly) Many are useless in non-human pwax tissues. I am a researcher and have no commercial "profile" ( other than accumulating Abcam points, lol) Please visit this site http://www.immunoportal.com/index.php and add any comments. Thanks Carlos ------------------------------ Message: 9 Date: Thu, 24 May 2007 21:52:50 +0100 From: "Carl Hobbs" Subject: [Histonet] Abcam survey To: "Histonet" Message-ID: <006f01c79e45$82c1f5f0$4101a8c0@carlba65530bda> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original They are interested in finding out how many of us Immunogeeks need Abs specifically directed against animal proteins. I have never encountered such interest before. Sure , many Abs are x-reactive: however, I have spent loads of money testing Abs out, that are human-reactive but their x-species reactivity is not known. ( I work with non-human sections, mostly) Many are useless in non-human pwax tissues. I am a researcher and have no commercial "profile" ( other than accumulating Abcam points, lol) Please visit this site http://www.immunoportal.com/index.php and add any comments. Thanks Carlos ------------------------------ Message: 10 Date: Thu, 24 May 2007 23:18:36 +0200 From: "tenny jin" Subject: [Histonet] Mouse specific M2/M6 antibody staining protocol To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=ISO-8859-1; format=flowed Dear all, Has anyone had some hand-on experience with mouse specific M2/M6 antibody (from Iowa Hybridoma bank) to detect mouse cells transplanted in the rat brain (cryosection)? I have trouble with the high background staining. If someone could share the staining protocol, I will appreciate it. Thanks in advance! Tenny ------------------------------ Message: 11 Date: Fri, 25 May 2007 10:22:32 +0200 From: "C.M. van der Loos" Subject: [Histonet] RE: IHC cytology specimens To: histonet@lists.utsouthwestern.edu Cc: mary.ann.deathridge@Vanderbilt.Edu Message-ID: <67bc0c67ba84.67ba8467bc0c@amc.uva.nl> Content-Type: text/plain; charset="us-ascii" Mary Ann, I am answering this from a research point of view, totally unaware of Her2neu guidelines or what so ever. We fixed cytospins in NBF (5 min, RT), washed them with tap water (5 min) and then to alcohol 70 (3 hours - overnight). Wash with tap water and perform HIER with appropriate buffer. We use half the time at max. temperature as for FFPE's. After cooling down, wash with tap water and perform IHC as with FFPE tissue sections (antibody, dilution, detection). I am aware this procedure as against all logics but it simple works. After all, 5 min NBF fixation cannot cause any cross linking and alcohol fixation certainly not. Therefore, HIER would not be necessary. But after testing we saw HIER was very essential! When I heard about this procedure I was very sceptical it would ever work. However, I was surprised to see how well the cells survive this harsh procedure and nicely stay at the glass. For some particular antigens we have even done this procedure successfully with cryostat tissue sections from fresh frozen blocks (section stays at the glass, morphology is fine)! Therefore folks: a theory is good to keep in mind, but testing is sometimes better. And, I also stopped with surprising myself about "strange" protocols. I hope this helps. Have a nice weekend, Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Thu, 24 May 2007 11:16:19 -0500 From: "Deathridge, Mary Ann" Subject: [Histonet] IHC cytology specimens To: Does anyone in histoland have a protocol for the prep of cytology specimens (cytospins, smears) for IHC? Formalin fix? how long? air dried? 95% alc fix? esp. since the new Her2nu guidelines Thanks in advance Thanks MaryAnn Deathridge, HT (ASCP) Supervisor, Histopathology Vanderbilt Un. Med. Ctr Nashville, TN ------------------------------ Message: 12 Date: Fri, 25 May 2007 11:53:56 +0100 From: "Ian Montgomery" Subject: [Histonet] Neurogenesis To: "Histonet" Message-ID: <001501c79eba$fe917640$4724d182@ibls.gla.ac.uk> Content-Type: text/plain; charset="iso-8859-1" I'm just about to start my literature search for information on a new neurogenesis project. In the first instance I'll only be looking at markers for new neurones so any hints and tips pointing me in the right direction would be welcome. Recently a topic has covered freezing tissue in iso-pentane cooled with solid CO2. This is also of interest for another project where I'll be studying skeletal muscle from salmon. My routine technique is iso-pentane cooled using liquid nitrogen and for an earlier pilot study this was the technique used. But, these salmon specimens are taken from pens in Scottish sea lochs so technically it's a wee bit tricky for the scientists freezing the specimens. Remember, this is Scotland where wind, rain and heaving seas are normal so using liquid nitrogen presents health and safety issues. A question for those who have frozen muscle using CO2 cooled iso-pentane? Can it be done successfully and repeatedly without ice-crystal damage? Obviously I'll get the people at the fish farm to try it before the experiments start but I don't want them to waste their time as in the commercial setting, time is money. I should also point out that Scottish salmon is like the whisky, the gift of the gods. Ian. Dr. Ian Montgomery, Histotechnology, IBLS Support Services, Thomson Building, University of Glasgow, Tel:01413398855 Extn: 8511. ------------------------------ Message: 13 Date: Fri, 25 May 2007 12:09:11 +0100 From: "matt prideaux \(RI\)" Subject: [Histonet] DAPI staining methacrylate sections To: Message-ID: <84DA9D8AC9B05F4B889E7C70238CB45104C52B7E@rie2ksrv1.ri.bbsrc.ac.uk> Content-Type: text/plain; charset="us-ascii" Hi everyone, I'm trying to find a method to examine osteocyte density in areas of new bone formation (between two calcein labels). The sections that we have are 200 micron thick sections of mouse tibia. We have a confocal microscope, which gives great images of the labels but the osteocytes are not easily seen. I have heard that DAPI would be an ideal stain for the osteocyte nuclei, but is it possible to stain methacrylate embedded sections with DAPI and if so does anyone have a method for this? Thanks, Matt Matt Prideaux Bone Biology Group Gene Function and Development Roslin Institute Roslin Midlothian EH25 9PS Tel: +44 131 5274 244/254 Institute Fax: +44 131 440 0434 Roslin Institute is a company limited by guarantee, registered in Scotland (registered number SC157100) and a Scottish Charity (registered number SC023592). Our registered office is at Roslin, Midlothian, EH25 9PS. VAT registration number 847380013. The information contained in this e-mail (including any attachments) is confidential and is intended for the use of the addressee only. The opinions expressed within this e-mail (including any attachments) are the opinions of the sender and do not necessarily constitute those of Roslin Institute (Edinburgh) ("the Institute") unless specifically stated by a sender who is duly authorised to do so on behalf of the Institute ------------------------------ Message: 14 Date: Fri, 25 May 2007 08:37:24 -0400 From: "Joanne Mauger" Subject: [Histonet] RE: IHC cytology specimens To: , Cc: mary.ann.deathridge@Vanderbilt.Edu Message-ID: Content-Type: text/plain; charset=US-ASCII Hi, I agree with Chris. NBF actually seems to help tissue stay on slide better. I will post fix a smear or cytospin in NBF for 15 mins. that has been previously fixed in alcohol. I do HIER as I would on FFPE. I have found that some antibodies will not show expression unless this post NBF fixation and retrieval has been done-eg. INI-1. I have also found retrieval necessary on thin prep cytospins that had been only fixed in sacamano,and 95% etoh. Trial and error is the key. Jo ------------------------------ Message: 15 Date: Fri, 25 May 2007 09:36:58 -0400 From: Tim Wheelock Subject: [Histonet] Custom made solutions To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <4656E67A.4040309@mclean.harvard.edu> Content-Type: text/plain; charset="iso-8859-1" Hi Everyone: Does anyone know of a company who makes custom-made solutions? I am finding it too time consuming and distracting to regularly make a cryoprotectant that has 5 ingredients and requires heat. Thanks, Tim Wheelock Harvard Brain Brain Belmont, MA P.S. The cryoprotectant requires: polyvinylpyrrolidone (PVP-40) sucrose sodium chloride sodium phosphate monobasic monohydrate sodium phosphate dibasic heptahydrate ethylene glycol and water. Any information, including protected health information (PHI), transmitted in this email is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential and/or exempt from disclosure under applicable Federal or State law. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon, PHI by persons or entities other than the intended recipient is prohibited. If you received this email in error, please contact the sender and delete the material from any computer. ------------------------------ Message: 16 Date: Fri, 25 May 2007 09:19:33 -0400 From: "Carole Fields" Subject: RE: [Histonet] Custom made solutions To: "Tim Wheelock" Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="iso-8859-1" I ran into the same situation and contacted Poly Scientific and they made up my solution and packaged it in prefilled containers. Also BBC will do the same....I learned after the fact. Either place is great and accommodating. Let me know if you are interested and I can give you the details. Carole Fields, HT,ASCP Pathology Supervisor Lexington Medical Center 2720 Sunset Blvd. W. Columbia, SC 29169 -----Original Message----- From: Tim Wheelock [mailto:twheelock@mclean.harvard.edu] Sent: Friday, May 25, 2007 9:37 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Custom made solutions Hi Everyone: Does anyone know of a company who makes custom-made solutions? I am finding it too time consuming and distracting to regularly make a cryoprotectant that has 5 ingredients and requires heat. Thanks, Tim Wheelock Harvard Brain Brain Belmont, MA P.S. The cryoprotectant requires: polyvinylpyrrolidone (PVP-40) sucrose sodium chloride sodium phosphate monobasic monohydrate sodium phosphate dibasic heptahydrate ethylene glycol and water. Any information, including protected health information (PHI), transmitted in this email is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential and/or exempt from disclosure under applicable Federal or State law. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon, PHI by persons or entities other than the intended recipient is prohibited. If you received this email in error, please contact the sender and delete the material from any computer. ------------------------------ Message: 17 Date: Fri, 25 May 2007 09:14:13 -0500 From: "Vickroy, Jim" Subject: [Histonet] test slides for detection kits To: Message-ID: Content-Type: text/plain; charset="us-ascii" How long are we required to keep test slides for documenting detection kits? Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ------------------------------ Message: 18 Date: Fri, 25 May 2007 15:29:54 +0100 From: "Alan Bright" Subject: RE: [Histonet] Neurogenesis To: "Ian Montgomery" , "Histonet" Message-ID: Content-Type: text/plain; charset="us-ascii" Dear Ian, On the subject of rapid freezing, I would like to make you aware that we manufacture the Bright Clini-RF Ultra low rapid freezer which is being used as a replacement for CO2 & liquid nitrogen. At present we have one type that's rapid freezing tank operates at -80 degs C and will shortly have another model for -100 degs C. There are also options available for controlling the temperature too. Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com Skype: dazzle0 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian Montgomery Sent: 25 May 2007 11:54 To: Histonet Subject: [Histonet] Neurogenesis I'm just about to start my literature search for information on a new neurogenesis project. In the first instance I'll only be looking at markers for new neurones so any hints and tips pointing me in the right direction would be welcome. Recently a topic has covered freezing tissue in iso-pentane cooled with solid CO2. This is also of interest for another project where I'll be studying skeletal muscle from salmon. My routine technique is iso-pentane cooled using liquid nitrogen and for an earlier pilot study this was the technique used. But, these salmon specimens are taken from pens in Scottish sea lochs so technically it's a wee bit tricky for the scientists freezing the specimens. Remember, this is Scotland where wind, rain and heaving seas are normal so using liquid nitrogen presents health and safety issues. A question for those who have frozen muscle using CO2 cooled iso-pentane? Can it be done successfully and repeatedly without ice-crystal damage? Obviously I'll get the people at the fish farm to try it before the experiments start but I don't want them to waste their time as in the commercial setting, time is money. I should also point out that Scottish salmon is like the whisky, the gift of the gods. Ian. Dr. Ian Montgomery, Histotechnology, IBLS Support Services, Thomson Building, University of Glasgow, Tel:01413398855 Extn: 8511. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Fri, 25 May 2007 09:09:31 -0600 From: "Breeden, Sara" Subject: [Histonet] Stainer Comparison To: Message-ID: <4D14F0FC9316DD41972D5F03C070908B8F449E@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="us-ascii" I have the opportunity to purchase an automatic stainer. I'm comparing the Leica Autostainer XL and the Sakura Tissue-Tek Prisma. If you had your 'druthers, which would you choose? Would you please reply OFF LIST with your pros and cons? Thank you! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 ------------------------------ Message: 20 Date: Fri, 25 May 2007 09:11:39 -0600 From: "Breeden, Sara" Subject: [Histonet] Donation of Slides? To: Message-ID: <4D14F0FC9316DD41972D5F03C070908B8F449F@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="us-ascii" Our local community college has a very popular vet(erinary) tech program; part of their training is making blood smears/touch preps/etc. They now wash and reuse slides several times and are in need of a donation of new slides. Is there a vendor out there that could donate a quantity of frosted, basic slides to this program? Please contact me directly if you would be willing to help out. Thanks very much! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 ------------------------------ Message: 21 Date: Fri, 25 May 2007 08:48:43 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Stainer Comparison To: "Breeden, Sara" , histonet@lists.utsouthwestern.edu Message-ID: <574862.65239.qm@web61214.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Sakura Tissue-Tek Prisma! Ren? J. "Breeden, Sara" wrote: I have the opportunity to purchase an automatic stainer. I'm comparing the Leica Autostainer XL and the Sakura Tissue-Tek Prisma. If you had your 'druthers, which would you choose? Would you please reply OFF LIST with your pros and cons? Thank you! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Don't pick lemons. See all the new 2007 cars at Yahoo! Autos. ------------------------------ Message: 22 Date: Fri, 25 May 2007 11:08:24 -0500 From: "Mike Pence" Subject: [Histonet] FW: Ventana Users To: Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C5E8@IS-E2K3.grhs.net> Content-Type: text/plain; charset="us-ascii" -----Original Message----- From: Mike Pence Sent: Friday, May 25, 2007 10:03 AM To: histonet-bounces@lists.utsouthwestern.edu Subject: Ventana Users I was wondering how many of you out there are using the Ventana Automated IHC System. I would like to build a network of like users to have as a resource when developing protocols in my IHC lab. We currently offer about 30 standard antibodies and I work up another 1 or 2 a month. It seems that when I call Ventana for protocol assistance they most times head me in the wrong direction. Thanks in advance for sharing your e-mail or thoughts with me. Mike ------------------------------ Message: 23 Date: Fri, 25 May 2007 10:35:32 -0600 From: "Liz Chlipala" Subject: [Histonet] help with some references To: Message-ID: <000001c79eea$b6e3b260$0d00a8c0@domain.Premier> Content-Type: text/plain; charset="us-ascii" Hello all I was wondering if there was anyone out there that had copies of two articles that they could fax or e-mail to me. One is from the Journal of Histotechnology from 1990, I have already requested this through the NSH web page but was hoping I could get it sooner. Its on species IHC cross reactivity and I think that Roberta Smith wrote it, but all I have is the following: Smith RA. J Histotechnology 1990;13(4): 255-69 And the other is from Applied Immunohistochemistry and Molecular Morphology also on antibody species cross reactivity Martin CA and Badran AF, Applied Immunohistochem 1998;6(2) :84-8 I don't have titles Thanks in advance and to all out here have a great Memorial Day weekend! Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 ------------------------------ Message: 24 Date: Fri, 25 May 2007 12:58:18 -0400 From: "Diana McCaig" Subject: [Histonet] gordon and sweetsd retic stain To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi I have done this stain successfuly for many years and the procedure has never changed. Yesterday, it failed to stain any retic and the repeat with all reagents prepared fresh gave the same results. Has anyone got any suggestions where I can start to investigate this? Diana ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 42, Issue 37 **************************************** Kaleida Health's economic impact on Western NY exceeds $2.2 BILLION annually. Check us out at: www.WesternNYshospitalofchoice.org CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. 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From KMH.02 <@t> ex.uchs.org Fri May 25 13:17:52 2007 From: KMH.02 <@t> ex.uchs.org (Hopkins, Karen) Date: Fri May 25 13:17:57 2007 Subject: [Histonet] Dako vs ventanna automated immunostainers Message-ID: <640B23A5DC4B234BB065E56F2DB30596028AB57A@uchex2ucmc.uchs.org> If anyone has an opinion they would like to share concerning the reliability of either the Ventana or Dako immunostainers I would appreciate hearing from you. I am only focusing on the reliability of results not the price, antibodies etc. Karen Hopkins Histology Supervisor Upper Chesapeake Medical Center & Harford Memorial Hospital kmh.02@ex.uchs.org From EWURDAK <@t> CSBSJU.EDU Fri May 25 13:18:54 2007 From: EWURDAK <@t> CSBSJU.EDU (Wurdak, Elizabeth) Date: Fri May 25 13:18:59 2007 Subject: [Histonet] Plastic Embedding In-Reply-To: <572057D3BDD52A46BD05BC6DA50686110CAE5E@MSGEBE22.mfad.mfroot.org> Message-ID: Hi Jim, We use JB-4 which is a glycol methacrylate-based polymer. To prevent overheating we keep the embedding mixture on ice and work with small (25ml) batches of the complete mix. Do not precool your molds as this may cause condensation and prevent even polymerization. We have not had any problems with overheating, but the last time we embedded our blocks turned out to be too hard to section with glass knives. It may be that we need to allow the blocks to polymerize at 4 degrees instead of room temperature or alter the ratio of solution A and B. Good luck, Elizabeth On 5/25/07 12:35 PM, "Herrick, James L." wrote: > Hello everyone, > > Does anyone have any experience with plastic embedding using Glycol > Methacrylate (GMA), Methyl Methacrylate (MMA) or other products that > could be used to embed vertebral specimens large enough that require > approximately 100 mLs of solution to embed completely. The problem I am > having using GMA is that the reaction becomes extremely hot and > polymerizes into a block full of holes and bubbles. I would really > appreciate any advice, protocols, etc., that you may have. Thanks. > > Jim > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MDiCarlo <@t> KaleidaHealth.Org Fri May 25 13:29:55 2007 From: MDiCarlo <@t> KaleidaHealth.Org (DiCarlo, Margaret) Date: Fri May 25 13:30:02 2007 Subject: [Histonet] flotation waterbaths Message-ID: <1B73766A27A1554CB2729B6432E81301195C7E@KALEXMB04.KaleidaHealth.org> Can anyone tell me where I can purchase a flotation water bath that the bowl is at least 8 ? inches wide and 4 inches deep? The reason I need this size bowl in a water bath is to transfer my tissue from a room temp water bowl on a 5 x 7 inch slide to a heated water bath with these dimensions so I have enough room to maneuver the tissue off the slide. I sure would appreciate your help. Thanks you. Peggy DiCarlo Orthopedics Bone Lab Buffalo General Hospital Buffalo, NY 14203 Kaleida Health's economic impact on Western NY exceeds $2.2 BILLION annually. Check us out at: www.WesternNYshospitalofchoice.org CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From elgerp <@t> kadlecmed.org Fri May 25 13:35:24 2007 From: elgerp <@t> kadlecmed.org (Elgert, Phil) Date: Fri May 25 13:35:29 2007 Subject: [Histonet] vacuoles in H&E stain Message-ID: Hi All, Here's a stumper. We deparaffinize for 10 minutes. We use Richard Allen hematolylin and eosin. We have 5 alcohols before going into our last Xylenes for clearing. So no water at the end. We still have a lot of nuclear vacuoles on the finished slides. I suspected processing but when I sent blocks to a lab across town they turned out fine. Has anybody seen this before? p.s. Have a great memorial day weekend. Phil Elgert Histology Supervisor Kadlec Medical Center 888 Swift Blvd. Richland, Wa 99352 ********************************************************************************************** IMPORTANT: The contents of this email and any attachments are confidential. They are intended for the named recipient(s) only. If you have received this email in error, please notify the system manager or the sender immediately and do not disclose the contents to anyone or make copies thereof. *** eSafe scanned this email for viruses, vandals, and malicious content. *** ********************************************************************************************** From PMonfils <@t> Lifespan.org Fri May 25 13:41:32 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri May 25 13:41:38 2007 Subject: [Histonet] Plastic Embedding In-Reply-To: <572057D3BDD52A46BD05BC6DA50686110CAE5E@MSGEBE22.mfad.mfroot.org> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273C93@LSRIEXCH1.lsmaster.lifespan.org> The only way to polymerize such large blocks without overheating is to polymerize under refrigeration. The block may take several weeks to polymerize, but a clear, bubble-free block can be produced in this way. The polymerization process is exothermic, and if the reaction procedes too fast you get into a cycle where the heat produced by the reaction cannot escape fast enough from the block, which makes the reaction go even faster, which produces even more heat, etc. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Herrick, James L. > Sent: Friday, May 25, 2007 1:35 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Plastic Embedding > > Hello everyone, > > Does anyone have any experience with plastic embedding using Glycol > Methacrylate (GMA), Methyl Methacrylate (MMA) or other products that > could be used to embed vertebral specimens large enough that require > approximately 100 mLs of solution to embed completely. The problem I am > having using GMA is that the reaction becomes extremely hot and > polymerizes into a block full of holes and bubbles. I would really > appreciate any advice, protocols, etc., that you may have. Thanks. > > Jim > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From JCollins <@t> palmbeachpath.com Fri May 25 13:42:12 2007 From: JCollins <@t> palmbeachpath.com (Judy Collins) Date: Fri May 25 13:42:17 2007 Subject: [Histonet] Vacuoles in H&E Stain Message-ID: We also have experienced vacuoles in the H&E stain in the past. We have come to believe that they are caused by the slides not being dry when they go into the oven. Moisture can then get trapped in the cells and cause this bubbling artifact. When we dry our slides for a full 30 minutes prior to the oven, the problem is practically nonexistent. Judy Collins Palm Beach Pathology From Charlene.Henry <@t> STJUDE.ORG Fri May 25 13:42:51 2007 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Fri May 25 13:42:54 2007 Subject: [Histonet] FW: Ventana Users. . In-Reply-To: <661949901A768E4F9CC16D8AF8F2838CA1C5E8@IS-E2K3.grhs.net> Message-ID: <5CB39BCA5724F349BCB748675C6CA1A21454345C@SJMEMXMB02.stjude.sjcrh.local> We currently have 2 Benchmark XT instruments and I have probably 140-150 antibodies already developed on these instruments so it sounds like a good idea to me. Charlene -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Friday, May 25, 2007 11:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Ventana Users. . -----Original Message----- From: Mike Pence Sent: Friday, May 25, 2007 10:03 AM To: histonet-bounces@lists.utsouthwestern.edu Subject: Ventana Users I was wondering how many of you out there are using the Ventana Automated IHC System. I would like to build a network of like users to have as a resource when developing protocols in my IHC lab. We currently offer about 30 standard antibodies and I work up another 1 or 2 a month. It seems that when I call Ventana for protocol assistance they most times head me in the wrong direction. Thanks in advance for sharing your e-mail or thoughts with me. Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charlene.Henry <@t> STJUDE.ORG Fri May 25 13:45:04 2007 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Fri May 25 13:45:09 2007 Subject: [Histonet] Dako vs ventanna automated immunostainers. . In-Reply-To: <640B23A5DC4B234BB065E56F2DB30596028AB57A@uchex2ucmc.uchs.org> Message-ID: <5CB39BCA5724F349BCB748675C6CA1A21454345D@SJMEMXMB02.stjude.sjcrh.local> We have both systems and they are both very reliable. I'll have to say that we have fewer repeats with the Ventana instruments simply because there are fewer areas where a technical error can occur. Both are excellent instruments. Charlene -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hopkins, Karen Sent: Friday, May 25, 2007 1:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dako vs ventanna automated immunostainers. . If anyone has an opinion they would like to share concerning the reliability of either the Ventana or Dako immunostainers I would appreciate hearing from you. I am only focusing on the reliability of results not the price, antibodies etc. Karen Hopkins Histology Supervisor Upper Chesapeake Medical Center & Harford Memorial Hospital kmh.02@ex.uchs.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MElliott <@t> mrl.ubc.ca Fri May 25 13:52:09 2007 From: MElliott <@t> mrl.ubc.ca (Mark Elliott) Date: Fri May 25 13:53:01 2007 Subject: [Histonet] Dako vs ventanna automated immunostainers Message-ID: <4656CDE9.11C6.00D6.0@mrl.ubc.ca> I have been using the DAKO stainer for 7-8 years and have had no problems with it. It is very reliable. Any problem we had we could trace to user error, not the machine. Mark Elliott >>> "Hopkins, Karen" 5/25/2007 11:17 AM >>> If anyone has an opinion they would like to share concerning the reliability of either the Ventana or Dako immunostainers I would appreciate hearing from you. I am only focusing on the reliability of results not the price, antibodies etc. Karen Hopkins Histology Supervisor Upper Chesapeake Medical Center & Harford Memorial Hospital kmh.02@ex.uchs.org ***CONFIDENTIALITY NOTICE*** This electronic message is intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From rjbuesa <@t> yahoo.com Fri May 25 14:18:30 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri May 25 14:18:39 2007 Subject: [Histonet] Dako vs ventanna automated immunostainers In-Reply-To: <640B23A5DC4B234BB065E56F2DB30596028AB57A@uchex2ucmc.uchs.org> Message-ID: <784632.41070.qm@web61224.mail.yahoo.com> Many times this same topic has been discussed in Histonet. You should try to go to the archives, but in "a nut shell", Dako has always come ahead of Ventana in the general concensus. Ren? J. "Hopkins, Karen" wrote: If anyone has an opinion they would like to share concerning the reliability of either the Ventana or Dako immunostainers I would appreciate hearing from you. I am only focusing on the reliability of results not the price, antibodies etc. Karen Hopkins Histology Supervisor Upper Chesapeake Medical Center & Harford Memorial Hospital kmh.02@ex.uchs.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get the free Yahoo! toolbar and rest assured with the added security of spyware protection. From pmcardle <@t> ebsciences.com Fri May 25 14:21:58 2007 From: pmcardle <@t> ebsciences.com (Phil McArdle) Date: Fri May 25 14:22:08 2007 Subject: [Histonet] Plastic Embedding In-Reply-To: <572057D3BDD52A46BD05BC6DA50686110CAE5E@MSGEBE22.mfad.mfroot.org> References: <572057D3BDD52A46BD05BC6DA50686110CAE5E@MSGEBE22.mfad.mfroot.org> Message-ID: <46573756.4080905@ebsciences.com> Hello James: Although I don't know if you're using a Heraeus-Kulzer Technovit product, I'm forwarding your question to their tech support people in Germany (we're the US distributor for Technovit). They've been very helpful in the past. Very best regards, Phil McArdle Herrick, James L. wrote: > Hello everyone, > > Does anyone have any experience with plastic embedding using Glycol > Methacrylate (GMA), Methyl Methacrylate (MMA) or other products that > could be used to embed vertebral specimens large enough that require > approximately 100 mLs of solution to embed completely. The problem I am > having using GMA is that the reaction becomes extremely hot and > polymerizes into a block full of holes and bubbles. I would really > appreciate any advice, protocols, etc., that you may have. Thanks. > > Jim > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Mobile: 860.597.6796 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com I skate to where the puck is going to be, not to where it's been. - Wayne Gretsky You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it, and it was probably my mistake anyway. So please do the right thing and make this e-mail go away. Thank you. From rjbuesa <@t> yahoo.com Fri May 25 14:25:05 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri May 25 14:25:13 2007 Subject: [Histonet] vacuoles in H&E stain In-Reply-To: Message-ID: <826428.24087.qm@web61211.mail.yahoo.com> The dryer is too hot! Ren? J. "Elgert, Phil" wrote: Hi All, Here's a stumper. We deparaffinize for 10 minutes. We use Richard Allen hematolylin and eosin. We have 5 alcohols before going into our last Xylenes for clearing. So no water at the end. We still have a lot of nuclear vacuoles on the finished slides. I suspected processing but when I sent blocks to a lab across town they turned out fine. Has anybody seen this before? p.s. Have a great memorial day weekend. Phil Elgert Histology Supervisor Kadlec Medical Center 888 Swift Blvd. Richland, Wa 99352 ********************************************************************************************** IMPORTANT: The contents of this email and any attachments are confidential. They are intended for the named recipient(s) only. If you have received this email in error, please notify the system manager or the sender immediately and do not disclose the contents to anyone or make copies thereof. *** eSafe scanned this email for viruses, vandals, and malicious content. *** ********************************************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Sick sense of humor? Visit Yahoo! TV's Comedy with an Edge to see what's on, when. From sbreeden <@t> nmda.nmsu.edu Fri May 25 14:29:36 2007 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri May 25 14:29:47 2007 Subject: [Histonet] Happy Friday and Thanks Message-ID: <4D14F0FC9316DD41972D5F03C070908B8F44B2@nmdamailsvr.nmda.ad.nmsu.edu> Thanks so much to those that took the time to give me input on the auto-stainer question. Input like this is priceless and I appreciate each of you. Thank you to the four separate entities that, while choosing to remain anonymous, donated enough slides to keep the Central New Mexico Community College Veterinary Technology program supplied for quite some time. You were very generous to have responded to my post. Everyone have a great three-day weekend and be safe. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 From psicurello <@t> mcvh-vcu.edu Fri May 25 15:00:43 2007 From: psicurello <@t> mcvh-vcu.edu (Paula Sicurello) Date: Fri May 25 15:01:08 2007 Subject: [Histonet] Plastic Embedding In-Reply-To: <572057D3BDD52A46BD05BC6DA50686110CAE5E@MSGEBE22.mfad.mfroot.org> References: <572057D3BDD52A46BD05BC6DA50686110CAE5E@MSGEBE22.mfad.mfroot.org> Message-ID: Hi Jim, The best way to polymerize the blocks is in the r room. I used to use ice but then you run into the possi water getting in the blocks. Paula NOTE: The information contained confidential and protected from disc message is not the intended recipient, you a any dissemination, distribution or copying of this strictly prohibited. If you have received this communicat error, please notify us immediately by replying to the message and d eleting it from your computer. -------------------------------------- VCU Health System http://www.vcuhealth.org From sheila_adey <@t> hotmail.com Fri May 25 15:18:55 2007 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Fri May 25 15:19:05 2007 Subject: [Histonet] Work hours In-Reply-To: <34BB307EFC9A65429BBB49E330675F7201B0B72E@LTA3VS003.ees.hhs.gov> Message-ID: We have 3 full time techs and we work 4 nine hour shifts with a 4hr half day. We rotate which day we get for the half day. Wed. Thurs. or Fri. Momdays and Tuesdays we all work 9 hours. We make sure all the maintenance gets done on the first 2 days of the week when we are all there. We love it. Sheila Adey HT MLT Port Huron Hospital Michigan >From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" >To: "Dawn Cowie" , "Mike Pence" >,"Rene J Buesa" ,"Patti Loykasek" >,"histonet" >Subject: RE: [Histonet] Work hours >Date: Thu, 24 May 2007 12:53:13 -0400 > >It's also a really nice way for the techs to save gas money and car wear. >It's the "green" thing to do! > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawn Cowie >Sent: Thursday, May 24, 2007 12:47 PM >To: Mike Pence; Rene J Buesa; Patti Loykasek; histonet >Subject: RE: [Histonet] Work hours > >We tried this at our lab. It worked really well for a while. We put >together a schedule that allowed the tech to be off on Monday - gave them a >3 day weekend. The next week a tech would be off on Friday - gave them a 3 >day weekend. The next week the tech would be off on Wednesday - they would >then work Mon, Tues - be off Wed then work Thurs and Fri. Each tech was >worked into the schedule this way. It was a bit difficult to work the >details, but everyone really enjoyed it. We developed a problem tho when we >lost 1 tech and 1 of the remaining techs needed to go back to the old >schedule because of family issues. We no longer had enough techs to make it >work so they all had to go back to the old schedule. > My feeling is that as long as you have the work and enough techs to make >it possible, its a good alternative to normal scheduling. Most of the techs >don't feel like they're working a full time job. > Just my 2 cents. Hope this gives you something useful. > > Dawn L. Cowie, HT (ASCP) Su > Pensacola Pathologists, PA > Pensacola, FL 32503 > 850-416-7251 > > >Mike Pence wrote: > How do you decide who gets which days off! Are they floating days? > >Mike > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J >Buesa >Sent: Wednesday, May 23, 2007 11:52 AM >To: Patti Loykasek; histonet >Subject: Re: [Histonet] Work hours > > >I have tried it. >Cons: If yu don't have a flow of specimens in 4 days equivalent to the flow >on 5 days, the personnel will be idling some time each day. If specimens or >workload is available everybody will have work to do. >Pros: it is fantastic having 3 days off weekly. Everybody likes that! >René J. > >Patti Loykasek >wrote: >I was wondering if anyone in histoland has experienced moving from a >schedule of standard 8 hour day/5 days a week to a 10 hour day/4 days a >week with techs having a day off during the week. If so, what are the pros >and cons of this type of schedule. I'm interested in feedback from both >bench techs and supervisor/managers. We are in the initial stages of >contemplating this and would appreciate info from anyone that has tried it. >Thank you. > > >Patti Loykasek BS, HTL, QIHC >PhenoPath Laboratories >Seattle, WA > > > >------------------------------------------------------------------------- >This e-mail message, including any attachments, is for the sole use of the >intended recipients and may contain privileged information. Any >unauthorized review, use, disclosure or distribution is prohibited. If >you are not the intended recipient, please contact the sender by e-mail >and destroy all copies of the original message, or you may call PhenoPath >Laboratories, Seattle, WA U.S.A. at (206) 374-9000. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Looking for a deal? Find great prices on flights and hotels with Yahoo! >FareChase. _______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ New Windows Live Hotmail is here. Upgrade for free and get a better look. www.newhotmail.ca?icid=WLHMENCA150 From slappycraw <@t> yahoo.com Fri May 25 18:47:13 2007 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Fri May 25 18:47:18 2007 Subject: [Histonet] Dako vs ventanna automated immunostainers In-Reply-To: <784632.41070.qm@web61224.mail.yahoo.com> Message-ID: <197513.2527.qm@web53609.mail.re2.yahoo.com> When working up a new antibody you need to have the flexability to pick and choose the solutions you want to try. The Dako has that, the Ventana does not, but for reliability they seem to be both like Hondas. Rene J Buesa wrote: Many times this same topic has been discussed in Histonet. You should try to go to the archives, but in "a nut shell", Dako has always come ahead of Ventana in the general concensus. Ren? J. "Hopkins, Karen" wrote: If anyone has an opinion they would like to share concerning the reliability of either the Ventana or Dako immunostainers I would appreciate hearing from you. I am only focusing on the reliability of results not the price, antibodies etc. Karen Hopkins Histology Supervisor Upper Chesapeake Medical Center & Harford Memorial Hospital kmh.02@ex.uchs.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get the free Yahoo! toolbar and rest assured with the added security of spyware protection. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a PS3 game guru. Get your game face on with the latest PS3 news and previews at Yahoo! Games. From hlukey <@t> msn.com Fri May 25 21:26:00 2007 From: hlukey <@t> msn.com (Hugh Luk) Date: Fri May 25 21:26:11 2007 Subject: [Histonet] vacuoles in H&E stain In-Reply-To: <826428.24087.qm@web61211.mail.yahoo.com> Message-ID: This was my first impression also. If your temperature is around 100 C, you are splitting the nuclear contents. Jackie O'Conner and I have seen it once before, from a reference lab we were using. The solution is simple: you just need to bring your dryer temperature down (lessening the temperature should increase your drying time). The only other thing that comes to mind is too much water in your fixative (hypotonic), but your experiment with the lab across town, discounted this. Good luck, Hugh Luk, HTL (ASCP) hluk@crch.hawaii.edu Pathology Shared Resources Lab Cancer Research Center of Hawaii >From: Rene J Buesa >To: "Elgert, Phil" , >histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] vacuoles in H&E stain >Date: Fri, 25 May 2007 12:25:05 -0700 (PDT) > >The dryer is too hot! > René J. > >"Elgert, Phil" wrote: > Hi All, > > > >Here's a stumper. We deparaffinize for 10 minutes. We use Richard Allen >hematolylin and eosin. We have 5 alcohols before going into our last >Xylenes for clearing. So no water at the end. We still have a lot of >nuclear vacuoles on the finished slides. I suspected processing but when >I sent blocks to a lab across town they turned out fine. Has anybody >seen this before? > > > >p.s. Have a great memorial day weekend. > > > >Phil Elgert > >Histology Supervisor > >Kadlec Medical Center > >888 Swift Blvd. > >Richland, Wa 99352 > > > > > > > > > >********************************************************************************************** >IMPORTANT: The contents of this email and any attachments are confidential. >They are intended for the >named recipient(s) only. >If you have received this email in error, please notify the system manager >or the sender immediately and do >not disclose the contents to anyone or make copies thereof. >*** eSafe scanned this email for viruses, vandals, and malicious content. >*** >********************************************************************************************** >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Sick sense of humor? Visit Yahoo! TV's Comedy with an Edge to see what's >on, when. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Catch suspicious messages before you open them—with Windows Live Hotmail. http://imagine-windowslive.com/hotmail/?locale=en-us&ocid=TXT_TAGHM_migration_HM_mini_protection_0507 From Gervaip <@t> aol.com Fri May 25 21:57:59 2007 From: Gervaip <@t> aol.com (Gervaip@aol.com) Date: Fri May 25 21:58:06 2007 Subject: [Histonet] processing of fatty tissue samples Message-ID: Hi, what is everyone doing in histo land when it comes to processing breast tissue? Pearl ************************************** See what's free at http://www.aol.com. From rjbuesa <@t> yahoo.com Sat May 26 08:28:29 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat May 26 08:28:35 2007 Subject: [Histonet] processing of fatty tissue samples In-Reply-To: Message-ID: <460443.49965.qm@web61224.mail.yahoo.com> Breast slices as thin as possible fixed at least 24 hours in 20:1 volume ratio of NBF. Well washed afterwards and with a protocol where EthOL and antemedium are at a rate time 1:1 Ren? J. Gervaip@aol.com wrote: Hi, what is everyone doing in histo land when it comes to processing breast tissue? Pearl ************************************** See what's free at http://www.aol.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- You snooze, you lose. Get messages ASAP with AutoCheck in the all-new Yahoo! Mail Beta. From loririchey <@t> comcast.net Sat May 26 09:21:03 2007 From: loririchey <@t> comcast.net (loririchey@comcast.net) Date: Sat May 26 09:21:07 2007 Subject: [Histonet] -20C acetone fixative Message-ID: <052620071421.9762.4658424F000A6359000026222207001641970A080C079D079D0104@comcast.net> I think I read, while studying for the IHC exam, that the reason the acetone is cold is to keep the tissue from washing off. It may not be necessary now because of the good quality charged slides available. -------------- Original message -------------- From: "Charles, Roger" > Does any know or remember why acetone, when used as a fixative, is > always used "ice cold" or in our case at -20C? > > > > > > Roger Charles > > Microbiologist > > Pennsylvania Veterinary Laboratory > > 2305 N Cameron St > > Harrisburg, PA 17110 > > 717-787-8808 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From loririchey <@t> comcast.net Sat May 26 09:51:18 2007 From: loririchey <@t> comcast.net (loririchey@comcast.net) Date: Sat May 26 09:51:24 2007 Subject: [Histonet] Work hours Message-ID: <052620071451.7092.46584966000778C100001BB42207001641970A080C079D079D0104@comcast.net> I've worked 4 ten hours shifts, and been involved in scheduling with several techs on these shifts. I won't say whether they are pros or cons, but just mention a few details to keep in mind. Most holidays fall on Mondays and Fridays, meaning that if it is someone's scheduled day off anyway, they take the holiday the following day, It's nice for the employee because it means a 4 day weekend. It's not great for the lab because it's a staffing shortage on tues. Holidays are also only 8 hours, so the remaining 2 hours of the "holiday off" comes out of vacation time. Heavy vacation times, like summer can also be a problem because of staffing shortages. It helps if people are flexible with their weekday off, and work as a team to make sure there is enough coverage. The nice thing about 4 tens, is that when the hours of the lab are long, and there is a large amount of workload at both the beginning and end of the shift, it works well having someone who has been there all day wra pping up loose ends, verses having to hand off work to the next shift. I think the bottom line is, that if the schedule works for the employee, and can work for the lab, it's a good thing, everyone is happy. -------------- Original message ---------------------- From: Patti Loykasek > I was wondering if anyone in histoland has experienced moving from a > schedule of standard 8 hour day/5 days a week to a 10 hour day/4 days a > week with techs having a day off during the week. If so, what are the pros > and cons of this type of schedule. I'm interested in feedback from both > bench techs and supervisor/managers. We are in the initial stages of > contemplating this and would appreciate info from anyone that has tried it. > Thank you. > > > Patti Loykasek BS, HTL, QIHC > PhenoPath Laboratories > Seattle, WA > > > > ------------------------------------------------------------------------- > This e-mail message, including any attachments, is for the sole use of > the intended recipients and may contain privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. If > you are not the intended recipient, please contact the sender by e-mail > and destroy all copies of the original message, or you may call PhenoPath > Laboratories, Seattle, WA U.S.A. at (206) 374-9000. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Sun May 27 13:53:41 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Sun May 27 13:53:58 2007 Subject: [Histonet] help with some references In-Reply-To: <000001c79eea$b6e3b260$0d00a8c0@domain.Premier> References: <000001c79eea$b6e3b260$0d00a8c0@domain.Premier> Message-ID: <46599B75020000770000608B@gwmail.harthosp.org> Hi Liz: I just faxed you both articles. Please let me know if the quality is O.K. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Liz Chlipala" 05/25/07 12:35 PM >>> Hello all I was wondering if there was anyone out there that had copies of two articles that they could fax or e-mail to me. One is from the Journal of Histotechnology from 1990, I have already requested this through the NSH web page but was hoping I could get it sooner. Its on species IHC cross reactivity and I think that Roberta Smith wrote it, but all I have is the following: Smith RA. J Histotechnology 1990;13(4): 255-69 And the other is from Applied Immunohistochemistry and Molecular Morphology also on antibody species cross reactivity Martin CA and Badran AF, Applied Immunohistochem 1998;6(2) :84-8 I don't have titles Thanks in advance and to all out here have a great Memorial Day weekend! Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From detmar <@t> mshri.on.ca Sun May 27 16:13:14 2007 From: detmar <@t> mshri.on.ca (Jacqui Detmar) Date: Sun May 27 16:13:29 2007 Subject: [Histonet] Veronal-acetate buffer Message-ID: Hi all. I would like to do some acid phosphatase staining, but the protocol I have includes a buffer that calls for sodium barbitone. I have searched a number of different sites for a possible substitute (including Histonet archives) and I can't seem to find one. Does anyone know if I can substitute another buffer for the veronal-acetate buffer? Thanks, Jacqui Detmar, Ph.D. candidate Samuel Lunenfeld Research Insitute, room 876 Mount Sinai Hospital 600 University Avenue Toronto, ON, Canada M5G 1X5 From brucea <@t> unimelb.edu.au Sun May 27 21:34:42 2007 From: brucea <@t> unimelb.edu.au (Bruce Abaloz) Date: Sun May 27 21:34:58 2007 Subject: [Histonet] Arsenic Laced Chicken/Cherax destructor Message-ID: Dear Colleagues :), I have an academic who has been feeding her Yabbies (fresh water Cray's)/Cherax Destructor - ...(love the name) with chicken laced arsenic. She is wanting /hoping for - a procedure that will show arsenic in the tissue/paraffin sections. I have searched Histology Books & also did a Google search to no avail. Any advice/offers of stains/procedures will be greatly appreciated. Many thanks in advance, Bruce in OZ -- BRUCE ABALOZ HISTOLOGIST PH:61383446282 DEPT. of ZOOLOGY EMAIL: brucea@unimelb.edu.au THE UNIVERSITY of MELBOURNE. FAX:61383447909 VICTORIA. AUSTRALIA 3010 Nobody Can Make You Feel Inferior Without YOUR Permission - Eleanor Roosevelt WHETHER YOU THINK YOU CAN-OR WHETHER YOU THINK YOU CAN'T-YOU'RE RIGHT!! Be reasonable... Demand the impossible..... <')))>>< <')))>>< <')))>>< <')))>>< P Please consider the environment before printing this e-mail. From AnthonyH <@t> chw.edu.au Sun May 27 22:27:25 2007 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun May 27 22:27:41 2007 Subject: [Histonet] Arsenic Laced Chicken/Cherax destructor Message-ID: This was from Histonet some years ago. The URL is: http://www.histosearch.com/homepages/TonyHenwood/hnet7.htm Stains for Arsenic During June 1999, a query was posted to Histonet asking for demonstration methods for Arsenic in tissues. Following is a summary: Roy Ellis (1) and Lynette Thibodeau (2) suggest Castel's Method: Fix in 10% formalin containing 2.5% copper sulfate for 5 days. Wash for 24 hours in running water. Process and embed in parffin wax. Deparaffinized sections show green granules of Scheele's green (CuHAsO3) which, though insoluble in water, is dissolved by acids and by ammonium hydroxide. By substituting copper acetate for the sulfate, the green granular paris green or cupric acetoarsenite is produced. Its solubilities are similar (Castel's method, Bull.Histol.Appliq. V13:106, 1936). A light safranin counterstain gives good contrast. Method courtesy of Lillie, 3rd edition, Histopathologic technic and practical histochemistry, page 445. John Kiernan (3) writes that arsenic compounds react with hydrogen sulphide to give insoluble As2S3. This is yellow, and unlikely to be visible, but could probably be amplified with a physical developer ("autometallography" or Timm's sulphide-silver method). However, this procedure demonstrates pretty well every metal that has an insoluble sulphide, so it would have no specificity for Arsenic. John also notes that there is a Japanese investigator, Y. Sumi, who has developed histochemical methods for inorganic substances based on combinations of chromogenic reagents with mixtures of "masking" agents that block the reactivity of elements other than the one you want to stain. His best known methods are for Cd and Hg, but he may have done something for As. Philip Oshel (4) suggests that if the arsenic is expected to be in nontrace amounts, you can detect, and maybe semi-quatify its presence with energy-dispersive x-ray spectroscopy (EDX or EDS) in a SEM or TEM. SEM might be better, as you could use "bulk" specimens--the small bits of tissue you prepare for sectioning. If there's enough arsenic, you could use paraffin-embedded sections in the SEM, but be aware that you could melt the paraffin. This can cause enough contamination in the column to antagonize the person in charge of the 'scope. For TEM, you'd have to use thin sections, but this would give better localization, if that's important. Prepare the specimen according to routine procedures for your tissues for either SEM or TEM. References: Roy Ellis (2/6/99) Lynette Thibodeau (2/6/99) John Kiernan (1/6/99) Philip Oshel (1/6/99) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bruce Abaloz Sent: Monday, 28 May 2007 12:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Arsenic Laced Chicken/Cherax destructor Dear Colleagues :), I have an academic who has been feeding her Yabbies (fresh water Cray's)/Cherax Destructor - ...(love the name) with chicken laced arsenic. She is wanting /hoping for - a procedure that will show arsenic in the tissue/paraffin sections. I have searched Histology Books & also did a Google search to no avail. Any advice/offers of stains/procedures will be greatly appreciated. Many thanks in advance, Bruce in OZ -- BRUCE ABALOZ HISTOLOGIST PH:61383446282 DEPT. of ZOOLOGY EMAIL: brucea@unimelb.edu.au THE UNIVERSITY of MELBOURNE. FAX:61383447909 VICTORIA. AUSTRALIA 3010 Nobody Can Make You Feel Inferior Without YOUR Permission - Eleanor Roosevelt WHETHER YOU THINK YOU CAN-OR WHETHER YOU THINK YOU CAN'T-YOU'RE RIGHT!! Be reasonable... Demand the impossible..... <')))>>< <')))>>< <')))>>< <')))>>< P Please consider the environment before printing this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From zumbor <@t> email.cs.nsw.gov.au Mon May 28 00:19:16 2007 From: zumbor <@t> email.cs.nsw.gov.au (Rosalba) Date: Mon May 28 00:24:46 2007 Subject: [Histonet] Hinged cassettes Message-ID: <01C7A13B.8EC9CA00.zumbor@email.cs.nsw.gov.au> Hi All, We have recently changed the type of cassettes we are using. We had been using hinged cassettes which all the pathologists like. The laboratory manager has said they are now unavailable and we have had to switch to one which the lid snaps off. Are the hinged cassettes still available and if so what brand are they. Thanks Rosalba Zumbo Supervisor Histology Dept Department of Forensic Medicine 42-50 Parramatta Rd Glebe NSW 2037 Australia PH: 61 2 85847842 FAX: 61 2 95664573 zumbor@email.cs.nsw.gov.au "This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please destroy it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of the Sydney South West Area Health Service." From Tony_Reilly <@t> health.qld.gov.au Mon May 28 01:12:45 2007 From: Tony_Reilly <@t> health.qld.gov.au (Anthony Reilly) Date: Mon May 28 01:13:17 2007 Subject: [Histonet] RE: IHC cytology specimens Message-ID: Hi I would also like to add that although it may seem contrary to how we treat paraffin sections air drying of your smears prior to fixation will greatly assist in keeping the cells on the slide and has no adverse affects on staining. regards Tony Reilly Chief Scientist Anatomical Pathology QHPS-Prince Charles Hospital Rode Rd Chermside Q 4032 Australia Ph: 07 3139 4543 Fax: 07 3193 4546 tony_reilly@health.qld.gov.au >>> "Joanne Mauger" 05/25/07 10:37 pm >>> Hi, I agree with Chris. NBF actually seems to help tissue stay on slide better. I will post fix a smear or cytospin in NBF for 15 mins. that has been previously fixed in alcohol. I do HIER as I would on FFPE. I have found that some antibodies will not show expression unless this post NBF fixation and retrieval has been done-eg. INI-1. I have also found retrieval necessary on thin prep cytospins that had been only fixed in sacamano,and 95% etoh. Trial and error is the key. Jo _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/ received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. **************************************************************** From rjbuesa <@t> yahoo.com Mon May 28 07:47:01 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon May 28 07:47:08 2007 Subject: [Histonet] Veronal-acetate buffer In-Reply-To: Message-ID: <843485.14948.qm@web61220.mail.yahoo.com> Jacqui: You are referring to the Michaelis veronal buffer for mast cells staining (Leder procedure). Unfortunately (as far as I know) this procedure works only with the veronal buffer (pH 6.3) and it is a component difficult to find (since it is a controlled substance). If you work in a hospital perhaps the pharmacy could have sodium barbital which is the difficult component to find. Ren? J. Jacqui Detmar wrote: Hi all. I would like to do some acid phosphatase staining, but the protocol I have includes a buffer that calls for sodium barbitone. I have searched a number of different sites for a possible substitute (including Histonet archives) and I can't seem to find one. Does anyone know if I can substitute another buffer for the veronal-acetate buffer? Thanks, Jacqui Detmar, Ph.D. candidate Samuel Lunenfeld Research Insitute, room 876 Mount Sinai Hospital 600 University Avenue Toronto, ON, Canada M5G 1X5 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get the free Yahoo! toolbar and rest assured with the added security of spyware protection. From caron_fournier <@t> yahoo.ca Mon May 28 10:11:09 2007 From: caron_fournier <@t> yahoo.ca (caron fournier) Date: Mon May 28 10:11:14 2007 Subject: [Histonet] Nikon NIS-Elements - digital camera software problem; software others use?? Message-ID: <187447.3001.qm@web35410.mail.mud.yahoo.com> Hi Gustave: I used to install and train people on imagine software and a number of times when we got new versions we had to make sure that the software was compatible with the service pack that the version of windows they were running was on. Check with the Nikon rep as they should know what service pack of windows you should be using with the software. If they do not know then they are not the people that you want to have to deal with on a regular basis. I used to sell for MediaCybernetics Image Pro Plus software and it was able to run the camera and do image analysis. They have a good setup for help with their tech support line and the reps are all trained in house as well as having a net forum for users to share ideas. Their website is www.mediacy.com check it out. Hope this helps. Caron Fournier, BSc, R.T. Department of Orthopaedics, Division of Orthopaedic Engineering Research, U.B.C. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of GT Hebert Sent: Thursday, May 24, 2007 11:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Nikon NIS-Elements - digital camera software problem; software others use?? Hello, Is anyone out there using a Nikon camera with NIS-elements (Advanced Research) software for digital imaging capture and digital image analysis?? Since buying the microscope system, I have been experiencing a multitude of software problems. In particular, during image 'capture', the computer will crash - a blue screen with white script would pop up and the computer subsequently will restart itself. Upon trying to open the software again, an error message would come up. I would have to restart the computer manually and open the software again. Nikon has come up with higher versions of the software and the updates have been installed, but as of yet, the problem has not been fixed. So my questions are as follows: Do you currently us NIS-Elements? If so, have you experienced similar issues? What image capturing software is the BEST both in terms of analysis and image capture? If I have to, I will eventually move on to a better system if this one continues to give me problems. Your answers and insight is much appreciated. Thank you in advance. Gustave Hebert Scientist II Wyeth Research Cambridge MA --------------------------------- 8:00? 8:25? 8:40? Find a flick in no time with theYahoo! Search movie showtime shortcut. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. 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See how smart SpamGuard is at giving junk email the boot with the All-new Yahoo! Mail at http://mrd.mail.yahoo.com/try_beta?.intl=ca From rchiovetti <@t> yahoo.com Mon May 28 12:41:16 2007 From: rchiovetti <@t> yahoo.com (Robert Chiovetti) Date: Mon May 28 12:41:22 2007 Subject: [Histonet] Nikon NIS-Elements - digital camera software problem; software others use?? Message-ID: <864403.70972.qm@web58903.mail.re1.yahoo.com> Gustave (and other Histonetters), Another good image capture and analysis package, thoroughly tested and compatible with all operating systems up through and including Windows Vista, is Pax-it. It's fully modular and configurable all the way from basic image capture to full-blown image analysis and archiving, exporting to spreadsheets, reports, templates, etc. with Intranet/Internet support, remote teleconferencing, and other useful features. We've had very good luck with this system. For more details, go to: www.paxit.com. Good luck! Cheers, Bob Chiovetti Robert (Bob) Chiovetti, Ph.D. Southwest Precision Instruments Arizona's Microscopy Resource Tucson, AZ 85710-3212 USA Tel./Fax 520-546-4986 www.swpinet.com Member, Arizona Small Business Association (www.asba.com) ------Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of GT Hebert Sent: Thursday, May 24, 2007 11:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Nikon NIS-Elements - digital camera software problem; software others use?? Hello, Is anyone out there using a Nikon camera with NIS-elements (Advanced Research) software for digital imaging capture and digital image analysis?? Since buying the microscope system, I have been experiencing a multitude of software problems. In particular, during image 'capture', the computer will crash - a blue screen with white script would pop up and the computer subsequently will restart itself. Upon trying to open the software again, an error message would come up. I would have to restart the computer manually and open the software again. Nikon has come up with higher versions of the software and the updates have been installed, but as of yet, the problem has not been fixed. So my questions are as follows: Do you currently us NIS-Elements? If so, have you experienced similar issues? What image capturing software is the BEST both in terms of analysis and image capture? If I have to, I will eventually move on to a better system if this one continues to give me problems. Your answers and insight is much appreciated. Thank you in advance. Gustave Hebert Scientist II Wyeth Research Cambridge MA ____________________________________________________________________________________Building a website is a piece of cake. Yahoo! Small Business gives you all the tools to get online. http://smallbusiness.yahoo.com/webhosting From flip-flop <@t> ngs.ru Mon May 28 13:42:23 2007 From: flip-flop <@t> ngs.ru (dimas) Date: Mon May 28 13:49:49 2007 Subject: [Histonet] rat brain parafin embedding Message-ID: Hello All. I am a student and I have problems whith rat brain paraffin embedding. To do good slices was very difficult. I tried to find protocols, but anywhere is wrote that brains were dehydrated throught a graded series of ethanol, clear in xylene and embedded according standard protocol. If anyone else has experience with rat brain paraffin embedding,could you send protocol. If it is not so difficult could you send fotos or just whrite how to make slices 1-2mm thick in order to orientation in atlas, and how brains embedded. Dmitriy Lanshakov Novosibirsk State University student flip-flop@ngs.ru ssdd@gorodok.net From marybeth.kaulahao <@t> thibodaux.com Mon May 28 15:02:35 2007 From: marybeth.kaulahao <@t> thibodaux.com (Marybeth Kaulahao) Date: Mon May 28 15:02:39 2007 Subject: [Histonet] ER/PR,Her2 Message-ID: <002101c7a163$22a82e70$7496010a@trmc.thibodaux.com> Help! What's going on with her2 and er/pr? Our pathologist went to a meeting and came back to tell us the breast must be in formalin 6 hrs but no more than 48 hrs. Now he wants to start the processor fri night for over the weekend and let the tissues process then sit in parafin from Saturday AM till monday AM. We process a LOT of small bladder and gi biopsies..is this good for them??What is anyone else out there doing? thanks Mary Beth From AnthonyH <@t> chw.edu.au Mon May 28 18:04:22 2007 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon May 28 18:04:34 2007 Subject: [Histonet] Veronal-acetate buffer Message-ID: ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** You can replace the barbitone buffer with an acetate buffer: About 20ml 0.2M Acetic acid plus 30ml 0.2M Sodium Acetate and pH to 4.8 (the pH we use for our acid phosphatases). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jacqui Detmar Sent: Monday, 28 May 2007 7:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Veronal-acetate buffer Hi all. I would like to do some acid phosphatase staining, but the protocol I have includes a buffer that calls for sodium barbitone. I have searched a number of different sites for a possible substitute (including Histonet archives) and I can't seem to find one. Does anyone know if I can substitute another buffer for the veronal-acetate buffer? Thanks, Jacqui Detmar, Ph.D. candidate Samuel Lunenfeld Research Insitute, room 876 Mount Sinai Hospital 600 University Avenue Toronto, ON, Canada M5G 1X5 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From renu <@t> akhileshaviation.com Mon May 28 20:20:46 2007 From: renu <@t> akhileshaviation.com (Renu) Date: Mon May 28 20:21:01 2007 Subject: [Histonet] (no subject) Message-ID: <2013C5E6286A46F885DD39456C1A643D@Akhil> I am going on vacation and want to be off the list until July 18th. Thanks. From louise.renton <@t> gmail.com Tue May 29 02:35:35 2007 From: louise.renton <@t> gmail.com (louise renton) Date: Tue May 29 02:35:40 2007 Subject: [Histonet] antibody concentration Message-ID: Hi this is further to a query I submitted last week. If starting to optimise a new antibody that has no dilution data supplied, what is a reasonable concentration to start off with.? Antibody is supplied at 0.2mg/ml and is polyclonal. many thanks Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From louise.renton <@t> gmail.com Tue May 29 02:37:19 2007 From: louise.renton <@t> gmail.com (louise renton) Date: Tue May 29 02:37:24 2007 Subject: [Histonet] Fwd: antibody concentration..oops Message-ID: Hi this is further to a query I submitted last week. If starting to optimise a new antibody that has no dilution data supplied, what is a reasonable concentration to start off with.? Antibody is supplied at 0.2mg/ml and is polyclonal. This is for immunohistochem on FFPE slides many thanks Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From TMcNemar <@t> lmhealth.org Tue May 29 05:06:10 2007 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Tue May 29 05:04:19 2007 Subject: [Histonet] suscribe Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F496@lmhsmail.lmhealth.org> Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org From sonya.martin <@t> soton.ac.uk Tue May 29 05:56:15 2007 From: sonya.martin <@t> soton.ac.uk (Martin S.) Date: Tue May 29 05:58:06 2007 Subject: [Histonet] RE: Mouse NK (PK136) Message-ID: <71437982F5B13A4D9A5B2669BDB89EE4023E35B4@ISS-CL-EX-V1.soton.ac.uk> Hi Gayle, Thanks for your advice - I've just seen your other messgae on Histonet (just back from hols so still catching up). I found one ref where they immersed the tissue in PLP, however in this case they then dehydated and embedded in paraffin. Cancer Immuno Immunother 2005 vol 54 pge229. I dont have easy access to paraffin embedding as I always work on frozen tissue so I'll try the immersion and freezing - if that doesnt work I'll do the perfusion. Will let you all know if I have any success! Sonya From rjbuesa <@t> yahoo.com Tue May 29 07:35:58 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue May 29 07:36:06 2007 Subject: [Histonet] rat brain parafin embedding In-Reply-To: Message-ID: <247184.34377.qm@web61221.mail.yahoo.com> Dimitry: To obtain slices that thin, the brain will have to be completely fixed in formalin first to provide "frimness" to the brain first. To guide you in slicing the brain you could use TWO very sharp knives blades tied together with a 1 mm thick piece of plastic between the 2 blades, that will give you a space 1 mm appart between the 2 blades and that could be your guide to prepare the 1 mm thick slices. The same if you want to make the 2 mm slices, just use a 2 mm thick piece of flat plastic. Once you have the slices you can use a general processing protocol, but reducing the times in ethanol to half. Hope this will help you. Ren? J. dimas wrote: Hello All. I am a student and I have problems whith rat brain paraffin embedding. To do good slices was very difficult. I tried to find protocols, but anywhere is wrote that brains were dehydrated throught a graded series of ethanol, clear in xylene and embedded according standard protocol. If anyone else has experience with rat brain paraffin embedding,could you send protocol. If it is not so difficult could you send fotos or just whrite how to make slices 1-2mm thick in order to orientation in atlas, and how brains embedded. Dmitriy Lanshakov Novosibirsk State University student flip-flop@ngs.ru ssdd@gorodok.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ready for the edge of your seat? Check out tonight's top picks on Yahoo! TV. From rjbuesa <@t> yahoo.com Tue May 29 07:38:22 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue May 29 07:38:26 2007 Subject: [Histonet] Fwd: antibody concentration..oops In-Reply-To: Message-ID: <155111.10616.qm@web61217.mail.yahoo.com> Just as a guide, check other policlonals you use that also have 0.2 mg/mL concentration of IgG and start with the sames concentrations you use on them to start your "checker-board" dilutions tests. Ren? J. louise renton wrote: Hi this is further to a query I submitted last week. If starting to optimise a new antibody that has no dilution data supplied, what is a reasonable concentration to start off with.? Antibody is supplied at 0.2mg/ml and is polyclonal. This is for immunohistochem on FFPE slides many thanks Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Luggage? GPS? Comic books? Check out fitting gifts for grads at Yahoo! Search. From cmiller <@t> physlab.com Tue May 29 08:00:30 2007 From: cmiller <@t> physlab.com (Cheri Miller) Date: Tue May 29 08:00:41 2007 Subject: [Histonet] (no subject) Message-ID: <005d01c7a1f1$59e7c420$3402a8c0@plab.local> I need to know if I can use Amylase from Barley malt as opposed to Amylase from Pancreas for Diastase. Does it matter for diastase purposes?? Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From rjbuesa <@t> yahoo.com Tue May 29 08:05:36 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue May 29 08:05:41 2007 Subject: [Histonet] (no subject) In-Reply-To: <005d01c7a1f1$59e7c420$3402a8c0@plab.local> Message-ID: <437261.44204.qm@web61221.mail.yahoo.com> Amilase is an enzyme that acts on glycogen, regardless of its origin. The concentration may be different and may affect the time you let it act, but usually diluted in the diastase buffer and incubated at 37?C during 1 hour, will be enough. Ren? J. Cheri Miller wrote: I need to know if I can use Amylase from Barley malt as opposed to Amylase from Pancreas for Diastase. Does it matter for diastase purposes?? Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! oneSearch: Finally, mobile search that gives answers, not web links. From ROrr <@t> enh.org Tue May 29 08:09:26 2007 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Tue May 29 08:09:32 2007 Subject: [Histonet] processing fatty tissue Message-ID: Message: 11 Date: Fri, 25 May 2007 22:57:59 EDT From: Gervaip@aol.com Subject: [Histonet] processing of fatty tissue samples To: histonet@pathology.swmed.edu Hi, what is everyone doing in histo land when it comes to processing breast tissue? Pearl Hi Pearl, We now keep a processor dedicated to (fatty) breast tissue. We do also run fatty tissue with this same process. You can check out the CAP guidelines for Breast tissue that may help with your process. We first had to decide if our fatty tissues were either partially fixed, partially cleared or a portion of both. Each time we had unacceptable tissue we would troubleshoot back the whole way to the OR. Our problem ended up as a combination of our Gross Staff (residents and PA's) submitting thicker pieces, cramming cassettes and not enough clearing time. We decided our fixation time was appropriate and well within the CAP guidelines. So we added more time in our 100% alcohols (you'll need to decide if you should add more time or change reagents more frequently or add an additional container of 100%) We also adjusted the processing schedule and added a third station of xylene. Our VIP has 4 paraffin baths and we use all of them with varied times. We now have excellent results with this set up. ~A simple reminder every hour on the hour to the Gross Room to "keep the tissue thin" helps everything run smoothly! (LOL) It is a great help for us to have the capability to separate our tissues in this manner with several processors. Becky Orr CLA,HT(ASCP)QIHC Assistant Manager, Anatomic Pathology Evanston Northwestern Healthcare 847-570-2763 From rjbuesa <@t> yahoo.com Tue May 29 08:22:59 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue May 29 08:23:03 2007 Subject: [Histonet] processing fatty tissue In-Reply-To: Message-ID: <746407.61558.qm@web61220.mail.yahoo.com> 1- thin slices 2- well fixed after the slices are prepared (at least 8 h) 3- use fresh alcohols for dehydration 4- extend somewaht (20%) the clearing steps time (time x 1.2), and 5- if possible use a 60?C paraffin, OR process with ethanol followed by a mixture of ethanol+propanol+mineral oil, and breats will cut "like butter". Ren? J. "Orr, Rebecca" wrote: Message: 11 Date: Fri, 25 May 2007 22:57:59 EDT From: Gervaip@aol.com Subject: [Histonet] processing of fatty tissue samples To: histonet@pathology.swmed.edu Hi, what is everyone doing in histo land when it comes to processing breast tissue? Pearl Hi Pearl, We now keep a processor dedicated to (fatty) breast tissue. We do also run fatty tissue with this same process. You can check out the CAP guidelines for Breast tissue that may help with your process. We first had to decide if our fatty tissues were either partially fixed, partially cleared or a portion of both. Each time we had unacceptable tissue we would troubleshoot back the whole way to the OR. Our problem ended up as a combination of our Gross Staff (residents and PA's) submitting thicker pieces, cramming cassettes and not enough clearing time. We decided our fixation time was appropriate and well within the CAP guidelines. So we added more time in our 100% alcohols (you'll need to decide if you should add more time or change reagents more frequently or add an additional container of 100%) We also adjusted the processing schedule and added a third station of xylene. Our VIP has 4 paraffin baths and we use all of them with varied times. We now have excellent results with this set up. ~A simple reminder every hour on the hour to the Gross Room to "keep the tissue thin" helps everything run smoothly! (LOL) It is a great help for us to have the capability to separate our tissues in this manner with several processors. Becky Orr CLA,HT(ASCP)QIHC Assistant Manager, Anatomic Pathology Evanston Northwestern Healthcare 847-570-2763 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Building a website is a piece of cake. Yahoo! Small Business gives you all the tools to get online. From JMcCormick <@t> schosp.org Tue May 29 08:39:45 2007 From: JMcCormick <@t> schosp.org (McCormick, James) Date: Tue May 29 08:40:21 2007 Subject: [Histonet] processing fatty tissue In-Reply-To: References: Message-ID: <913FAC2B773C19488E26AE6572180FA50C49911E@exch01.schosp.org> Rebecca, As the pathologist inventor of tissue-tek I have been "fighting" fatty tissue all of my LONG professional life. You are correct that the BEST of all answers is for the PA or pathologist to cut tissue sections 2-3mm thick. It helps to use COLD/refrigerated fatty tissue for this purpose (much like the butcher slices "leaf lard" to place on the surface of an expensive tenderloin!). A VERY sharp knife/blade is also helpful. After designing the original tissue-tek cassette with metal covers and round holes, I designed cassettes with an increased number of rectangular holes in both the bottoms and tops, and then the cassettes with attached covers were added by Lab Tek to eliminate the cleaning of metal covers. These changes DID NOT eliminate problems in processing FATTY tissue because the circulation of processing fluids was not materially improved. Finally, and with a particular concern for processing fatty tissue.....I invented TurbOflow cassettes to increase the circulation of processing fluids with a turbine-like "swish" movement thru the side walls of the cassette. This has helped somewhat in the processing of breast and other fatty tissues because of the increased flow across the cassette contained tissues. HOWEVER....nothing is better than a 2-3mm thick tissue sample placed in the processing cassette by the PA or Pathologist....or Resident in training. I have been PREACHING this same sermon for more than 50 years and apparently not all have been listening. Good Luck and Kindest regards. J.B.McCormick M.D. CSO McCormick Scientific 110 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Orr, Rebecca Sent: Tuesday, May 29, 2007 8:09 AM To: histonet@lists.utsouthwestern.edu Cc: Gervaip@aol.com Subject: [Histonet] processing fatty tissue Message: 11 Date: Fri, 25 May 2007 22:57:59 EDT From: Gervaip@aol.com Subject: [Histonet] processing of fatty tissue samples To: histonet@pathology.swmed.edu Hi, what is everyone doing in histo land when it comes to processing breast tissue? Pearl Hi Pearl, We now keep a processor dedicated to (fatty) breast tissue. We do also run fatty tissue with this same process. You can check out the CAP guidelines for Breast tissue that may help with your process. We first had to decide if our fatty tissues were either partially fixed, partially cleared or a portion of both. Each time we had unacceptable tissue we would troubleshoot back the whole way to the OR. Our problem ended up as a combination of our Gross Staff (residents and PA's) submitting thicker pieces, cramming cassettes and not enough clearing time. We decided our fixation time was appropriate and well within the CAP guidelines. So we added more time in our 100% alcohols (you'll need to decide if you should add more time or change reagents more frequently or add an additional container of 100%) We also adjusted the processing schedule and added a third station of xylene. Our VIP has 4 paraffin baths and we use all of them with varied times. We now have excellent results with this set up. ~A simple reminder every hour on the hour to the Gross Room to "keep the tissue thin" helps everything run smoothly! (LOL) It is a great help for us to have the capability to separate our tissues in this manner with several processors. Becky Orr CLA,HT(ASCP)QIHC Assistant Manager, Anatomic Pathology Evanston Northwestern Healthcare 847-570-2763 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. From JMcCormick <@t> schosp.org Tue May 29 08:41:44 2007 From: JMcCormick <@t> schosp.org (McCormick, James) Date: Tue May 29 08:42:03 2007 Subject: [Histonet] processing fatty tissue In-Reply-To: <746407.61558.qm@web61220.mail.yahoo.com> References: <746407.61558.qm@web61220.mail.yahoo.com> Message-ID: <913FAC2B773C19488E26AE6572180FA50C499125@exch01.schosp.org> AMEN.......... J.B.McCormick, M.D. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, May 29, 2007 8:23 AM To: Orr, Rebecca; histonet@lists.utsouthwestern.edu Cc: Gervaip@aol.com Subject: Re: [Histonet] processing fatty tissue 1- thin slices 2- well fixed after the slices are prepared (at least 8 h) 3- use fresh alcohols for dehydration 4- extend somewaht (20%) the clearing steps time (time x 1.2), and 5- if possible use a 60?C paraffin, OR process with ethanol followed by a mixture of ethanol+propanol+mineral oil, and breats will cut "like butter". Ren? J. "Orr, Rebecca" wrote: Message: 11 Date: Fri, 25 May 2007 22:57:59 EDT From: Gervaip@aol.com Subject: [Histonet] processing of fatty tissue samples To: histonet@pathology.swmed.edu Hi, what is everyone doing in histo land when it comes to processing breast tissue? Pearl Hi Pearl, We now keep a processor dedicated to (fatty) breast tissue. We do also run fatty tissue with this same process. You can check out the CAP guidelines for Breast tissue that may help with your process. We first had to decide if our fatty tissues were either partially fixed, partially cleared or a portion of both. Each time we had unacceptable tissue we would troubleshoot back the whole way to the OR. Our problem ended up as a combination of our Gross Staff (residents and PA's) submitting thicker pieces, cramming cassettes and not enough clearing time. We decided our fixation time was appropriate and well within the CAP guidelines. So we added more time in our 100% alcohols (you'll need to decide if you should add more time or change reagents more frequently or add an additional container of 100%) We also adjusted the processing schedule and added a third station of xylene. Our VIP has 4 paraffin baths and we use all of them with varied times. We now have excellent results with this set up. ~A simple reminder every hour on the hour to the Gross Room to "keep the tissue thin" helps everything run smoothly! (LOL) It is a great help for us to have the capability to separate our tissues in this manner with several processors. Becky Orr CLA,HT(ASCP)QIHC Assistant Manager, Anatomic Pathology Evanston Northwestern Healthcare 847-570-2763 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Building a website is a piece of cake. Yahoo! Small Business gives you all the tools to get online. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. From cfranssen <@t> nsalabs.com Tue May 29 09:11:54 2007 From: cfranssen <@t> nsalabs.com (Franssen, Dr. Catherine) Date: Tue May 29 09:12:21 2007 Subject: [Histonet] neurotoxin history Message-ID: <26C60098CBC18C4F97C2514517EDB439053139AF@VS6.EXCHPROD.USA.NET> Hi Histonetters. I'm wondering if anyone out there with an interest in history knows which neurotoxin was the first to be described pathologically? Thanks for your time. Catherine Catherine Lowry Franssen, Ph.D. NeuroScience Associates 10915 Lake Ridge Drive Knoxville, TN 37934 tel. 865-675-2245 cel. 865-712-9314 fax. 865-675-2787 From anthony <@t> histotechexchange.com Tue May 29 09:51:36 2007 From: anthony <@t> histotechexchange.com (anthony@histotechexchange.com) Date: Tue May 29 09:52:05 2007 Subject: [Histonet] Alfa & Beta Amylase In-Reply-To: <437261.44204.qm@web61221.mail.yahoo.com> References: <437261.44204.qm@web61221.mail.yahoo.com> Message-ID: <1319.71.51.6.248.1180450319.squirrel@host4.wfdns.com> Dear Rene: There are two types of Amylase, Alfa & Beta. Alfa will release Glucose and maltose but Beta will only release maltose. You can get Diastase (meaning it contains both Alfa & Beta)from Malt; try Fisher Scientific. I would look into your procedure and ask your doctor if he is happy with you saying you use Diastase when you are actually only using Alfa Amylase. If you have the New Bancroft and Stevens it is on page 173. Good luck and putting the subject in the header will get more people to respond. Yours truly, Anthony Williams Histotech Exchange LLC 19 Whitmore St. Lexington, VA 24450 T 1 877 464 8911 F 1 540 301 0071 anthony@Histotechexchange.com www.histotechexchange.com > concentration may be different and may affect the time you let it act, but > usually diluted in the diastase buffer and incubated at 37?C during 1 > hour, will be enough. > Ren? J. > > Cheri Miller wrote: > I need to know if I can use Amylase from Barley malt as opposed to > Amylase > from Pancreas for Diastase. Does it matter for diastase purposes?? > > Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne > > > > > > > > > > > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If > you are not the addressee intended / indicated or agent responsible for > delivering it to the addressee, you are hereby notified that you are in > possession of confidential and privileged information. Any dissemination, > distribution, or copying of this e-mail is strictly prohibited. If you > have received this message in error, please notify the sender immediately > and delete this email from your system. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Yahoo! oneSearch: Finally, mobile search that gives answers, not web > links. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gcallis <@t> montana.edu Tue May 29 10:33:23 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue May 29 10:33:15 2007 Subject: [Histonet] rat brain parafin embedding In-Reply-To: References: Message-ID: <6.0.0.22.1.20070529092452.01b804c8@gemini.msu.montana.edu> To cut precisely oriented slices of rodent brains, use a brain matrix. These can be purchased as either plastic or metal matrix. Go to this website, www.myneurolab.com, and click on brain matrices. The brain fits into the well of matrix in a specific way, so the front part of the brain fits in the depression for that portion of the brain. After that, you can do precise slices with a thin sharp blade. There are also rodent brain atlases on internet to show you orientations and some beautifully stained sections. There are more suppliers of these available. The slices are then embedded flat to maintain a labeled sequence of the slices. ) At 12:42 PM 5/28/2007, you wrote: >Hello All. > > I am a student and I have problems whith rat brain paraffin embedding. > To do good slices was very difficult. >I tried to find protocols, but anywhere is wrote that brains were >dehydrated throught a graded series of ethanol, clear in xylene and >embedded according standard protocol. >If anyone else has experience with rat brain paraffin embedding,could you send >protocol. >If it is not so difficult could you send fotos or just whrite how to make >slices 1-2mm thick in order to orientation in atlas, and how brains embedded. > >Dmitriy Lanshakov >Novosibirsk State University >student >flip-flop@ngs.ru >ssdd@gorodok.net > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From gcallis <@t> montana.edu Tue May 29 10:53:38 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue May 29 10:53:27 2007 Subject: [Histonet] antibody concentration In-Reply-To: References: Message-ID: <6.0.0.22.1.20070529095126.01be9e88@gemini.msu.montana.edu> Louise, We start with a target concentration of 10 ug/ml, and I have seen 20ug/ml per DAKO/Boenisch Manual recommendation too, then do serial dilutions. At 01:35 AM 5/29/2007, you wrote: >Hi >this is further to a query I submitted last week. If starting to optimise a >new antibody that has no dilution data supplied, what is a reasonable >concentration to start off with.? Antibody is supplied at 0.2mg/ml and is >polyclonal. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From jfish <@t> gladstone.ucsf.edu Tue May 29 11:27:50 2007 From: jfish <@t> gladstone.ucsf.edu (Jo Dee Fish) Date: Tue May 29 11:28:03 2007 Subject: [Histonet] Hinged cassettes In-Reply-To: <01C7A13B.8EC9CA00.zumbor@email.cs.nsw.gov.au> Message-ID: <002d01c7a20e$4cb39d70$2e0d010a@JFISH> Rosalba, I just saw some at our California Society for Histotechnology Symposium. They were featured at the McCormick booth, and also Pacific Southwest Lab Equipment, Inc has them, their URL is www.pls-equip.com. Try them, I'm sorry I don't have a catalog number. Good luck, Jo Dee PS I am not a representative of either of these companies. Jo Dee Fish Research Technologist III Gladstone Institute of Cardiovascular Disease Telephone: (415) 734-2567 Fax: (415) 355-0824 E-mail: jfish@gladstone.ucsf.edu Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rosalba Sent: Sunday, May 27, 2007 10:19 PM To: Histonet (E-mail) Subject: [Histonet] Hinged cassettes Hi All, We have recently changed the type of cassettes we are using. We had been using hinged cassettes which all the pathologists like. The laboratory manager has said they are now unavailable and we have had to switch to one which the lid snaps off. Are the hinged cassettes still available and if so what brand are they. Thanks Rosalba Zumbo Supervisor Histology Dept Department of Forensic Medicine 42-50 Parramatta Rd Glebe NSW 2037 Australia PH: 61 2 85847842 FAX: 61 2 95664573 zumbor@email.cs.nsw.gov.au "This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please destroy it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of the Sydney South West Area Health Service." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Tue May 29 11:59:03 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue May 29 11:58:49 2007 Subject: [Histonet] Hinged cassettes In-Reply-To: <002d01c7a20e$4cb39d70$2e0d010a@JFISH> References: <01C7A13B.8EC9CA00.zumbor@email.cs.nsw.gov.au> <002d01c7a20e$4cb39d70$2e0d010a@JFISH> Message-ID: <6.0.0.22.1.20070529105412.01b3aec8@gemini.msu.montana.edu> ThermoFisher also has them. Fisher Tissue Path IV Tissue Cassettes, regular, biopsy and or under the Richard Allan Histoscreen Tissue/biopsy cassettes. Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 From kmerriam2003 <@t> yahoo.com Tue May 29 12:19:00 2007 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Tue May 29 12:19:02 2007 Subject: [Histonet] Fluorescent X-gal substrate Message-ID: <233286.20809.qm@web50307.mail.re2.yahoo.com> Hello everyone, Does anyone know of a fluorescently-labeled X-gal substrate that is available for histochemical staining of frozen sections? We currently are doing b-gal IHC on FFPE and X-gal histochemistry (blue substrate) for frozens), but we would like to do fluorescent X-gal HISTOchemistry (not IHC), if possible. I am not sure if this is even a possibility. Kim Kim Merriam Cambridge, MA ____________________________________________________________________________________Get the free Yahoo! toolbar and rest assured with the added security of spyware protection. http://new.toolbar.yahoo.com/toolbar/features/norton/index.php From BoozerKA <@t> ah.org Tue May 29 12:26:08 2007 From: BoozerKA <@t> ah.org (Kathleen Boozer) Date: Tue May 29 12:26:51 2007 Subject: [Histonet] Gold seed implants Message-ID: <465BFFBF.4AA8.00C0.0@ah.org> A pathologist just asked me how to easily find "gold seed" implants out of a chest wall mass the size of a big steak. After cutting through the tissue and only finding one, he suggested a strong acid to dissolve all the tissue. Oncology told me you can see it under a fluoroscope, but still, the pathologist would have to dissect them out. Maybe a funeral home incinerator...ha! Any ideas? Kathy Boozer Histology Adventist Medical Center From carl.hobbs <@t> kcl.ac.uk Tue May 29 12:33:55 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Tue May 29 12:34:17 2007 Subject: [Histonet] re: Ab concentration Message-ID: <005e01c7a217$88a1a170$4101a8c0@carlba65530bda> Ignore Ab concentration, in the 1st instance: just carry out a titration of your Ab reagent. For eg: 1/10, 50, 100, 500, 1000. If the Ab seems to be positive, next immunorun can finetune positivity to achieve, ultimately, optimal signal:noise ratio. NB: [Ab] can be misleading: it tells you nothing of the Ab's affinity for it's Ag, within a given system ( eg: pwax sections) Empiricism rules, OK? ;-) Carl From dassog <@t> evergreen.edu Tue May 29 12:39:30 2007 From: dassog <@t> evergreen.edu (Dasso, Greg (staff)) Date: Tue May 29 12:40:49 2007 Subject: [Histonet] Gold seed implants References: <465BFFBF.4AA8.00C0.0@ah.org> Message-ID: <3872E8431D06E545AC955BED177CB6F601604F97@oak.evergreen.edu> KOH/H2O2 digestion might work. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Kathleen Boozer Sent: Tue 5/29/2007 10:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gold seed implants A pathologist just asked me how to easily find "gold seed" implants out of a chest wall mass the size of a big steak. After cutting through the tissue and only finding one, he suggested a strong acid to dissolve all the tissue. Oncology told me you can see it under a fluoroscope, but still, the pathologist would have to dissect them out. Maybe a funeral home incinerator...ha! Any ideas? Kathy Boozer Histology Adventist Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Tue May 29 12:43:06 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Tue May 29 12:43:17 2007 Subject: [Histonet] Gold seed implants In-Reply-To: <465BFFBF.4AA8.00C0.0@ah.org> Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C5F0@IS-E2K3.grhs.net> Are these something that simple x-ray would pick up? If so, cut the tissue into sections and spread on a Rubbermaid lid. Have x-ray use the mammo unit and shoot the pieces. Pick the pieces with the seeds and there you have it. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathleen Boozer Sent: Tuesday, May 29, 2007 12:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gold seed implants A pathologist just asked me how to easily find "gold seed" implants out of a chest wall mass the size of a big steak. After cutting through the tissue and only finding one, he suggested a strong acid to dissolve all the tissue. Oncology told me you can see it under a fluoroscope, but still, the pathologist would have to dissect them out. Maybe a funeral home incinerator...ha! Any ideas? Kathy Boozer Histology Adventist Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Tue May 29 12:56:49 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue May 29 12:56:40 2007 Subject: [Histonet] Fluorescent X-gal substrate In-Reply-To: <233286.20809.qm@web50307.mail.re2.yahoo.com> References: <233286.20809.qm@web50307.mail.re2.yahoo.com> Message-ID: <6.0.0.22.1.20070529115312.01b3e5e0@gemini.msu.montana.edu> Kim, I have seen it. Try this company, Inalco Pharmaceuticals. www.inalcopharm.com, or 800-709-6776. We have not tried it to date. Also, Molecular Probes may have info, maybe ask their tech services if you can't find it on their website. At 11:19 AM 5/29/2007, you wrote: >Hello everyone, > >Does anyone know of a fluorescently-labeled X-gal substrate that is >available for histochemical staining of frozen sections? We currently are >doing b-gal IHC on FFPE and X-gal histochemistry (blue substrate) for >frozens), but we would like to do fluorescent X-gal HISTOchemistry (not >IHC), if possible. I am not sure if this is even a possibility. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From anh2006 <@t> med.cornell.edu Tue May 29 13:48:30 2007 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Tue May 29 13:48:44 2007 Subject: [Histonet] re: Ab concentration In-Reply-To: <005e01c7a217$88a1a170$4101a8c0@carlba65530bda> References: <005e01c7a217$88a1a170$4101a8c0@carlba65530bda> Message-ID: I couldn't disagree more. Assuming this is purified IgG and not antiserum, you need to know and go by antibody concentration to know how much control IgG to add and how to interpret your results from one experiment to the next. Antibody dilution alone is meaningless and near impossible to interpret without controls at the same IgG. At 6:33 PM +0100 5/29/07, Carl Hobbs wrote: >Ignore Ab concentration, in the 1st instance: just carry out a >titration of your Ab reagent. >For eg: 1/10, 50, 100, 500, 1000. >If the Ab seems to be positive, next immunorun can finetune >positivity to achieve, ultimately, optimal signal:noise ratio. >NB: [Ab] can be misleading: it tells you nothing of the Ab's >affinity for it's Ag, within a given system ( eg: pwax sections) >Empiricism rules, OK? >;-) >Carl > -- From settembr <@t> umdnj.edu Tue May 29 14:40:33 2007 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Tue May 29 14:41:49 2007 Subject: [Histonet] looking for hPL Message-ID: Is any one using human Placental Lactogen (hPL)? What vendor? Is it IVD? I need it for formalin fixed paraffin embedded human tissue and I can't seem to find it anywhere. Many people seem to have discontinued it. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA From HoustonR <@t> chi.osu.edu Tue May 29 15:15:23 2007 From: HoustonR <@t> chi.osu.edu (Houston, Ronald) Date: Tue May 29 15:15:58 2007 Subject: [Histonet] Cell Block preparation Message-ID: <979FF5962E234F45B06CF0DB7C1AABB20FEC1DD5@chi2k3ms01.columbuschildrens.net> Would anyone care to comment on the pros and cons of preparing cell blocks using the Plamsa/thrombin technique and the Agar method which is more prominent in the European field? I am particularly interested in the prevalence of background staining in ICC. Does the use of plasma interfere with interpretation of the staining results? I know there have been reports of extraneous tissue being found in cell blocks coming from a commercially prepared clotting agent. Thanks Ronnie Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Columbus Children's Hospital 700 Children's Drive Columbus, OH 43205 ----------------------------------------- Confidentiality Notice: This e-mail message, from Children's Hospital, Columbus, Ohio, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes pursuant to Children's Hospital's confidentiality policies. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From elockman <@t> apsemail.com Tue May 29 15:24:25 2007 From: elockman <@t> apsemail.com (Emily Lockman) Date: Tue May 29 15:24:29 2007 Subject: [Histonet] Calcium stains Message-ID: <037BDA8D37760D49A2A23D1C877EA8C9233C16@apsdc01.aps.dom> I work in a research facility in Minneapolis, MN. I am trying to locate information on IHC stains for calcium. If possible, I would like to know if there are stains available for osteocytes, osteoclasts, and osteoblasts. If anyone has any information, I would greatly appreciate it. Additionally, if there is a way to test for the gender of cells, for example, if a piece of bone marrow from a male donor is implanted into a female, is there a way to see which cells are from the male vs. female? Emily M. Lockman, HT (ASCP) Histotechnologist I, Pathology Services American Preclinical Services, LLC (APS) 8945 Evergreen Boulevard Minneapolis, MN 55433 Phone: 763-717-7990 ext. 2025 (necropsy) ext. 2006 (desk) Fax: 763-717-2042 elockman@apsemail.com From bhewlett <@t> cogeco.ca Tue May 29 16:19:44 2007 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Tue May 29 16:19:51 2007 Subject: [Histonet] Cell Block preparation (lengthy) References: <979FF5962E234F45B06CF0DB7C1AABB20FEC1DD5@chi2k3ms01.columbuschildrens.net> Message-ID: <003401c7a237$14a3d250$6500a8c0@mainbox> Ronnie, I have compared a number of methods for preparing cell blocks for IHC and have the following comment; Overall, I much prefer the plasma/thrombin method. Primarily because freshly harvested cells can be collected in plasma, transported, aliquoted and the cell concentration easily adjusted, prior to addition of thrombin. Furthermore, unfixed cells are now completely surrounded by the same proteinaceous intercellular matrix found in all solid tissues. This proteinaceous matrix is a necessary and crucial component of all fixation reactions in tissue and actively participates in the fixation process. To my way of thinking, cell blocks produced this way more closely mimic solid tissues for IHC control purposes! Once clotted (15 mins including retraction) the cell blocks can be optimally fixed and processed. We have used this method for preparing numerous cell blocks for IHC. The only background staining seen is a specific background, due to secondary reagents being insufficiently absorbed against human Ig's, this is easily corrected as necessary. One caveat, this type of specific background will interfere with interpretation of staining for Human Ig's, Kappa/Lambda light chains and albumen, particularly if agonal imbibition by dying cells occurs. Bryan ----- Original Message ----- From: "Houston, Ronald" To: Sent: Tuesday, May 29, 2007 4:15 PM Subject: [Histonet] Cell Block preparation Would anyone care to comment on the pros and cons of preparing cell blocks using the Plamsa/thrombin technique and the Agar method which is more prominent in the European field? I am particularly interested in the prevalence of background staining in ICC. Does the use of plasma interfere with interpretation of the staining results? I know there have been reports of extraneous tissue being found in cell blocks coming from a commercially prepared clotting agent. Thanks Ronnie Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Columbus Children's Hospital 700 Children's Drive Columbus, OH 43205 ----------------------------------------- Confidentiality Notice: This e-mail message, from Children's Hospital, Columbus, Ohio, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes pursuant to Children's Hospital's confidentiality policies. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Tue May 29 16:57:05 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue May 29 16:56:54 2007 Subject: [Histonet] Calcium stains In-Reply-To: <037BDA8D37760D49A2A23D1C877EA8C9233C16@apsdc01.aps.dom> References: <037BDA8D37760D49A2A23D1C877EA8C9233C16@apsdc01.aps.dom> Message-ID: <6.0.0.22.1.20070529155525.01b400f8@gemini.msu.montana.edu> Molecular Probes has an array of fluorescent probes for calcium but I am not sure they work on tissue sections, and probably are NOT immunostaining. At 02:24 PM 5/29/2007, you wrote: >I work in a research facility in Minneapolis, MN. I am trying to locate >information on IHC stains for calcium. If possible, I would like to know >if there are stains available for osteocytes, osteoclasts, and >osteoblasts. If anyone has any information, I would greatly appreciate >it. > > > >Additionally, if there is a way to test for the gender of cells, for >example, if a piece of bone marrow from a male donor is implanted into a >female, is there a way to see which cells are from the male vs. female? > > > >Emily M. Lockman, HT (ASCP) > >Histotechnologist I, Pathology Services > >American Preclinical Services, LLC (APS) > >8945 Evergreen Boulevard > >Minneapolis, MN 55433 > >Phone: 763-717-7990 > > ext. 2025 (necropsy) > > ext. 2006 (desk) > >Fax: 763-717-2042 > >elockman@apsemail.com > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From Tony_Reilly <@t> health.qld.gov.au Tue May 29 18:48:20 2007 From: Tony_Reilly <@t> health.qld.gov.au (Anthony Reilly) Date: Tue May 29 18:48:44 2007 Subject: [Histonet] Cell Block preparation Message-ID: I have been using the agar method for 30 years. The best features are that they are quick and easy to make and when embedding the agar is clear so any pellet or collection of cells in the agar can be easily seen and the agar button orientated to get the best capture of cells. Also the agar is readily available from the Microbiology dept of our laboratory. The cons are that agar buttons will not process on a short cycle if the specimen is urgent and I would always ensure that the cells are fixed prior to putting into the agar as the heat of molten agar will affect fresh cells. This will affect not only the IHC but the appearance of the cells in a H&E. regards Tony Reilly Chief Scientist Anatomical Pathology QHPS-Prince Charles Hospital Rode Rd Chermside Q 4032 Australia Ph: 07 3139 4543 Fax: 07 3193 4546 tony_reilly@health.qld.gov.au >>> "Houston, Ronald" 05/30/07 6:15 am >>> Would anyone care to comment on the pros and cons of preparing cell blocks using the Plamsa/thrombin technique and the Agar method which is more prominent in the European field? I am particularly interested in the prevalence of background staining in ICC. Does the use of plasma interfere with interpretation of the staining results? I know there have been reports of extraneous tissue being found in cell blocks coming from a commercially prepared clotting agent. Thanks Ronnie Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Columbus Children's Hospital 700 Children's Drive Columbus, OH 43205 ----------------------------------------- Confidentiality Notice: This e-mail message, from Children's Hospital, Columbus, Ohio, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes pursuant to Children's Hospital's confidentiality policies. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/ received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. **************************************************************** From AnthonyH <@t> chw.edu.au Tue May 29 18:51:38 2007 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue May 29 18:51:52 2007 Subject: [Histonet] Cell Block preparation Message-ID: I have used both agar amd plasma clot preparations for IPX. If the sample has been fixed in solution prior to cell block preparation then I have often had to resort to agar cell block preparations. Also if the sample has a high concentration of proteases eg bile fluid, then often the plasma clot won't form. IPXs can often be problematical in agar cell blocks, probably due to the deleterious effect of the hot agar. Plasma Cell Blocks are definitely more consistent Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Wednesday, 30 May 2007 6:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cell Block preparation Would anyone care to comment on the pros and cons of preparing cell blocks using the Plamsa/thrombin technique and the Agar method which is more prominent in the European field? I am particularly interested in the prevalence of background staining in ICC. Does the use of plasma interfere with interpretation of the staining results? I know there have been reports of extraneous tissue being found in cell blocks coming from a commercially prepared clotting agent. Thanks Ronnie Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Columbus Children's Hospital 700 Children's Drive Columbus, OH 43205 ----------------------------------------- Confidentiality Notice: This e-mail message, from Children's Hospital, Columbus, Ohio, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes pursuant to Children's Hospital's confidentiality policies. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From rsrichmond <@t> aol.com Tue May 29 20:00:59 2007 From: rsrichmond <@t> aol.com (rsrichmond@aol.com) Date: Tue May 29 20:01:06 2007 Subject: [Histonet] Re: ER/PR, HER2 In-Reply-To: <200705291301.9c1465c5c4b2be@rly-me06.mx.aol.com> References: <200705291301.9c1465c5c4b2be@rly-me06.mx.aol.com> Message-ID: <8C9706D1F0A7CAD-88C-9268@FWM-M41.sysops.aol.com> Mary Beth Kaulahao asks: >>Help! What's going on with HER2 and ER/PR? Our pathologist went to a meeting and came back to tell us the breast [tissue] must be in formalin 6 hrs but no more than 48 hrs. Now he wants to start the processor fri night for over the weekend and let the tissues process then sit in paraffin from Saturday AM till Monday AM. We process a LOT of small bladder and GI biopsies.. is this good for them?? What is anyone else out there doing?<< Everyone out there is very confused. You can read a rather muddled account of the problem in CAP Today a month or so ago. Clearly you can't let tissue sit in hot paraffin for two days, though some are advocating doing it. Any deviation from fixation time (or fixation in anything other than neutral buffered formalin) results in a clinically unproven procedure. But if the bureaucrats and managers want tissue to sit in hot paraffin for two days, then we're going to have to say no to them. It's unproven, but I would think that transferring tissue to 70% ethanol after overnight fixation in NBF, and holding it in ethanol until it can be processed, would be preferable. A better, though unorthodox solution would be to do a small processor run Friday night, and have the pathologist-on-call embed the tissue on Saturday morning. About time pathologists learned to embed! Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From srwilkes <@t> gmail.com Tue May 29 23:31:27 2007 From: srwilkes <@t> gmail.com (Steven Wilkes) Date: Tue May 29 23:31:31 2007 Subject: [Histonet] New York Technologist License Exam Message-ID: <8e5827cf0705292131v4fc74e9dlb801177bae7ef237@mail.gmail.com> Hi everyone Has anyone taken th New York Technologist License exam recently? Any words of advice you can shared with a someone studying for the exam? did you find it difficult? How many questions? What was the format? How did you study for the exam? books? guides? Any information would be greatly appreciated. Thanks in advance. On a different note... anyone in the NYC area want to study together for the exam? Steven srwilkes@hotmail.com From louise.renton <@t> gmail.com Wed May 30 02:13:53 2007 From: louise.renton <@t> gmail.com (louise renton) Date: Wed May 30 02:14:02 2007 Subject: [Histonet] ihc titre - thanks Message-ID: Thanks to all those who gave me ideas on where to start titering the polyclonal Ab. Histonetters ROCK!!!! (BTW, I am so excited, ther's a teensy tiny chance that I might get sppnsorship to go to the NSH meeting in Denver. I so look forward to putting faces to names!) best regards Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From barry_m <@t> ozemail.com.au Wed May 30 04:08:42 2007 From: barry_m <@t> ozemail.com.au (Barry Madigan) Date: Wed May 30 04:09:16 2007 Subject: [Histonet] non specific staining with HER2 CISH Message-ID: <000001c7a29a$29959280$7cc0b780$@com.au> With the HER2 CISH procedure from Invitrogen we are experiencing non specific staining on breast specimens with DAB. The staining appears to be mucinous exudate from tumour cells. Just wondering if anyone else has also experienced this as well. It certainly doesn't help with counting. Regards Barry Madigan Immunohistochemistry QHPS-RBH Brisbane Australia From Jeannette.Mitchell <@t> vtmednet.org Wed May 30 05:36:19 2007 From: Jeannette.Mitchell <@t> vtmednet.org (Mitchell, Jeannette M.) Date: Wed May 30 05:36:27 2007 Subject: [Histonet] histology regs Message-ID: <8C7B2CE85A8CBA49B3FE05DE478412ED028E3E43@FAHC14.fahc.fletcherallen.org> Help !! Does anyone know what fire codes REALLY apply to histology labs and how did one justify having a tissue processor in less than 500 square feet without a fire barrier around it ? The reason I ask is : We have a 10x22' tissue processing workroom with 5 routine processors, one recycling system, and explosion cabinets containing xylene, toluene, and ethanol. Overall we have approx. 70 gallons of etoh and solvents in this 220' room. We have been told by an independent industrial hygienist that we should only have 4.4 gallons (or 2 gallons per 100 square feet) of combustibles in this room and each tissue processor should be separated from the other by "1 hour construction" (some type of barrier that would take an hour to burn through) according to NFPA 99 Chapter 11. I have visited many histology labs in the New England area and I have never seen tissue processors separated by 1 hour burn barriers or processors in separate 500 sq. foot rooms (10 gallons of combustibles per processor would require this space). I have asked for a second opinion as this is not practical, rational, or feasible. PLEASE share your experience with fire regs in your lab ~ Thanks ! Jude Carpenter, BS, HTL (ASCP) Supervisor of Histology/Surgical Pathology/Autopsy Fletcher Allen Healthcare EP2-101/ACC 111 Colchester Ave. Burlington, VT 05401 (802)847-5116 FAX (802)847-4155 jude.carpenter@vtmednet.org Confidentiality Notice: This message, and any attachments, may contain information that is confidential, privileged, and/or protected from disclosure under state and federal laws that deal with the privacy and security of medical information. If you received this message in error or through inappropriate means, please reply to this message to notify the Sender that the message was received by you in error, and then permanently delete this message from all storage media, without forwarding or retaining a copy. From mthomas <@t> littonlab.com Wed May 30 06:03:06 2007 From: mthomas <@t> littonlab.com (Marla Thomas) Date: Wed May 30 06:03:17 2007 Subject: [Histonet] histology regs Message-ID: <001401c7a2aa$19ff24c0$9d35a8c0@LittonPath.local> I do not know of any regs specifically for Histology, but this link will take you to the OSHA reg for Flammable storage: http://www.osha.gov/pls/oshaweb/owadisp.show_document?p_table=STANDARDS &p_id=10673 Keep in mind this is just for storage, flammables on processors are not considered storage. Marla Thomas, HT(ASCP) HIPAA/Compliance/IT Manager Litton Pathology Associates, PC 700 NW Hunter Drive Blue Springs, MO 64015 This e-mail, including attachments, may include confidential and/or proprietary information, and may be used only by the person or entity to which it is addressed. If the reader of this e-mail is not the intended recipient or his/her authorized agent, the reader is hereby notified that any dissemination, distribution or copying of this e-mail is prohibited. If you have received this e-mail in error, please notify the sender by replying to this message and delete this e-mail immediately. From HoustonR <@t> chi.osu.edu Wed May 30 06:59:12 2007 From: HoustonR <@t> chi.osu.edu (Houston, Ronald) Date: Wed May 30 06:59:40 2007 Subject: [Histonet] histology regs In-Reply-To: <8C7B2CE85A8CBA49B3FE05DE478412ED028E3E43@FAHC14.fahc.fletcherallen.org> Message-ID: <979FF5962E234F45B06CF0DB7C1AABB20FEC1DDB@chi2k3ms01.columbuschildrens.net> Apart from the regs associated with OSHA, this really is the decision of your local fire marshal. Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Columbus Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 houstonr@chi.osu.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell, Jeannette M. Sent: Wednesday, May 30, 2007 6:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] histology regs Help !! Does anyone know what fire codes REALLY apply to histology labs and how did one justify having a tissue processor in less than 500 square feet without a fire barrier around it ? The reason I ask is : We have a 10x22' tissue processing workroom with 5 routine processors, one recycling system, and explosion cabinets containing xylene, toluene, and ethanol. Overall we have approx. 70 gallons of etoh and solvents in this 220' room. We have been told by an independent industrial hygienist that we should only have 4.4 gallons (or 2 gallons per 100 square feet) of combustibles in this room and each tissue processor should be separated from the other by "1 hour construction" (some type of barrier that would take an hour to burn through) according to NFPA 99 Chapter 11. I have visited many histology labs in the New England area and I have never seen tissue processors separated by 1 hour burn barriers or processors in separate 500 sq. foot rooms (10 gallons of combustibles per processor would require this space). I have asked for a second opinion as this is not practical, rational, or feasible. PLEASE share your experience with fire regs in your lab ~ Thanks ! Jude Carpenter, BS, HTL (ASCP) Supervisor of Histology/Surgical Pathology/Autopsy Fletcher Allen Healthcare EP2-101/ACC 111 Colchester Ave. Burlington, VT 05401 (802)847-5116 FAX (802)847-4155 jude.carpenter@vtmednet.org Confidentiality Notice: This message, and any attachments, may contain information that is confidential, privileged, and/or protected from disclosure under state and federal laws that deal with the privacy and security of medical information. If you received this message in error or through inappropriate means, please reply to this message to notify the Sender that the message was received by you in error, and then permanently delete this message from all storage media, without forwarding or retaining a copy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: This e-mail message, from Children's Hospital, Columbus, Ohio, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes pursuant to Children's Hospital's confidentiality policies. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From rjbuesa <@t> yahoo.com Wed May 30 07:46:11 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 30 07:46:16 2007 Subject: [Histonet] histology regs In-Reply-To: <8C7B2CE85A8CBA49B3FE05DE478412ED028E3E43@FAHC14.fahc.fletcherallen.org> Message-ID: <554771.65115.qm@web61217.mail.yahoo.com> Jeanette: All tissue processors have fumes controls, meaning that any flammable fumes (alcohols or xylene) are controlled and pass through either water or chacoal filters, determining that they do not escape to the environment and cannot be ignited. That is why you can have tissue processors in small spaces and even several close together. Ren? J. "Mitchell, Jeannette M." wrote: Help !! Does anyone know what fire codes REALLY apply to histology labs and how did one justify having a tissue processor in less than 500 square feet without a fire barrier around it ? The reason I ask is : We have a 10x22' tissue processing workroom with 5 routine processors, one recycling system, and explosion cabinets containing xylene, toluene, and ethanol. Overall we have approx. 70 gallons of etoh and solvents in this 220' room. We have been told by an independent industrial hygienist that we should only have 4.4 gallons (or 2 gallons per 100 square feet) of combustibles in this room and each tissue processor should be separated from the other by "1 hour construction" (some type of barrier that would take an hour to burn through) according to NFPA 99 Chapter 11. I have visited many histology labs in the New England area and I have never seen tissue processors separated by 1 hour burn barriers or processors in separate 500 sq. foot rooms (10 gallons of combustibles per processor would require this space). I have asked for a second opinion as this is not practical, rational, or feasible. PLEASE share your experience with fire regs in your lab ~ Thanks ! Jude Carpenter, BS, HTL (ASCP) Supervisor of Histology/Surgical Pathology/Autopsy Fletcher Allen Healthcare EP2-101/ACC 111 Colchester Ave. Burlington, VT 05401 (802)847-5116 FAX (802)847-4155 jude.carpenter@vtmednet.org Confidentiality Notice: This message, and any attachments, may contain information that is confidential, privileged, and/or protected from disclosure under state and federal laws that deal with the privacy and security of medical information. If you received this message in error or through inappropriate means, please reply to this message to notify the Sender that the message was received by you in error, and then permanently delete this message from all storage media, without forwarding or retaining a copy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get the Yahoo! toolbar and be alerted to new email wherever you're surfing. From karenadams <@t> comcast.net Wed May 30 08:15:56 2007 From: karenadams <@t> comcast.net (karenadams@comcast.net) Date: Wed May 30 08:16:03 2007 Subject: [Histonet] Re: recycler usage.... Message-ID: <053020071315.4893.465D790C000BD4280000131D22070245539C030E0B0E020A9D0E05@comcast.net> Hello all........My lab has a 2.5 gallon demo- alcohol/xylene recycler we have been using for 1 month. Initially we felt like we would need to units to dedicate each unit to either alcohol or xylene...however we found out that 1 is perfect for the quantities we have every day. We currently recycle 2-3 times per day, 5 days a week. Anyone know of any ill effects for using the unit that often....I thought that is what it was for......please feel free to contact me off list if you like....Thanks...karen -- Karen Adams Pathology Laboratories West 9303 Park West Blvd Knoxville, TN 37923 (865) 690-2111 FAX (865) 691-1623 From mpence <@t> grhs.net Wed May 30 08:23:06 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Wed May 30 08:23:17 2007 Subject: [Histonet] histology regs In-Reply-To: <8C7B2CE85A8CBA49B3FE05DE478412ED028E3E43@FAHC14.fahc.fletcherallen.org> Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C5F2@IS-E2K3.grhs.net> First, I would assume that all your tissue processors are closed systems. There are no codes as to separating tissue processors if they are closed systems. Second, is the flammable cabinet you have rated for 70 gallon? It should have a self closing door on it. Third, do you have an AFE system for this room? I would agree that the bottom line is what the local fire code and marshall have to say about the area. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell, Jeannette M. Sent: Wednesday, May 30, 2007 5:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] histology regs Help !! Does anyone know what fire codes REALLY apply to histology labs and how did one justify having a tissue processor in less than 500 square feet without a fire barrier around it ? The reason I ask is : We have a 10x22' tissue processing workroom with 5 routine processors, one recycling system, and explosion cabinets containing xylene, toluene, and ethanol. Overall we have approx. 70 gallons of etoh and solvents in this 220' room. We have been told by an independent industrial hygienist that we should only have 4.4 gallons (or 2 gallons per 100 square feet) of combustibles in this room and each tissue processor should be separated from the other by "1 hour construction" (some type of barrier that would take an hour to burn through) according to NFPA 99 Chapter 11. I have visited many histology labs in the New England area and I have never seen tissue processors separated by 1 hour burn barriers or processors in separate 500 sq. foot rooms (10 gallons of combustibles per processor would require this space). I have asked for a second opinion as this is not practical, rational, or feasible. PLEASE share your experience with fire regs in your lab ~ Thanks ! Jude Carpenter, BS, HTL (ASCP) Supervisor of Histology/Surgical Pathology/Autopsy Fletcher Allen Healthcare EP2-101/ACC 111 Colchester Ave. Burlington, VT 05401 (802)847-5116 FAX (802)847-4155 jude.carpenter@vtmednet.org Confidentiality Notice: This message, and any attachments, may contain information that is confidential, privileged, and/or protected from disclosure under state and federal laws that deal with the privacy and security of medical information. If you received this message in error or through inappropriate means, please reply to this message to notify the Sender that the message was received by you in error, and then permanently delete this message from all storage media, without forwarding or retaining a copy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Wed May 30 08:25:06 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Wed May 30 08:25:11 2007 Subject: [Histonet] Re: recycler usage.... In-Reply-To: <053020071315.4893.465D790C000BD4280000131D22070245539C030E0B0E020A9D0E05@comcast.net> Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C5F3@IS-E2K3.grhs.net> What brand? That could make a difference. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of karenadams@comcast.net Sent: Wednesday, May 30, 2007 8:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: recycler usage.... Hello all........My lab has a 2.5 gallon demo- alcohol/xylene recycler we have been using for 1 month. Initially we felt like we would need to units to dedicate each unit to either alcohol or xylene...however we found out that 1 is perfect for the quantities we have every day. We currently recycle 2-3 times per day, 5 days a week. Anyone know of any ill effects for using the unit that often....I thought that is what it was for......please feel free to contact me off list if you like....Thanks...karen -- Karen Adams Pathology Laboratories West 9303 Park West Blvd Knoxville, TN 37923 (865) 690-2111 FAX (865) 691-1623 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charles.Embrey <@t> carle.com Wed May 30 09:08:03 2007 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Wed May 30 09:08:09 2007 Subject: [Histonet] Re: recycler usage.... In-Reply-To: <661949901A768E4F9CC16D8AF8F2838CA1C5F3@IS-E2K3.grhs.net> Message-ID: <44780C571F28624DBB446DE55C4D733A1FE4E9@EXCHANGEBE1.carle.com> If it's a CBG it will handle it with no problem. Our two units are real workhorses. Charles Embrey, PA(ASCP) Histology Manager Carle Clinic, Illinois -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, May 30, 2007 8:25 AM To: karenadams@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: recycler usage.... What brand? That could make a difference. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of karenadams@comcast.net Sent: Wednesday, May 30, 2007 8:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: recycler usage.... Hello all........My lab has a 2.5 gallon demo- alcohol/xylene recycler we have been using for 1 month. Initially we felt like we would need to units to dedicate each unit to either alcohol or xylene...however we found out that 1 is perfect for the quantities we have every day. We currently recycle 2-3 times per day, 5 days a week. Anyone know of any ill effects for using the unit that often....I thought that is what it was for......please feel free to contact me off list if you like....Thanks...karen -- Karen Adams Pathology Laboratories West 9303 Park West Blvd Knoxville, TN 37923 (865) 690-2111 FAX (865) 691-1623 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Heather.D.Renko <@t> osfhealthcare.org Wed May 30 09:09:49 2007 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Wed May 30 09:10:07 2007 Subject: [Histonet] gender analaysis-bone marrow Message-ID: <40026EDDE64CDA47AB382C52619ACD3C077754D0@pmc-rfd-mx01.intranet.osfnet.org> You can determine the gender of your cells through chromosomal analaysis/cytogenetics. Just as long as you have a nucleus you're good to go. Heather Renko Histology Coordinator OSF Saint Anthony Medical Center 5666 E. State St., Rockford, IL 61108 Phone: (815) 395-5410 Fax: (815) 395-5364 ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From jcox90 <@t> yahoo.com Wed May 30 09:43:12 2007 From: jcox90 <@t> yahoo.com (Jill Cox) Date: Wed May 30 09:43:18 2007 Subject: [Histonet] Histology lab Manager position in Texas Message-ID: <143295.66009.qm@web56815.mail.re3.yahoo.com> Hi Histonetters, We have a wonderful opportunity for a working Histology Lab Manager in Athens Texas for the right individual. This is a brand new Private small laboratory, one Awsome Pathologist, great setting. If interested please call 903-675-0080 or email your resume to jcox90@yahoo.com From bwhitaker <@t> brownpathology.com Wed May 30 10:37:10 2007 From: bwhitaker <@t> brownpathology.com (Bonnie Whitaker) Date: Wed May 30 10:35:43 2007 Subject: [Histonet] PRN Grossing salary Message-ID: <002001c7a2d0$63e5cb90$3601a8c0@brownpathology.net> Hi Everyone, I would like to ask if anyone is using (eligible) techs and/or PA's to gross small specimens on a PRN basis, and if so, what hourly rate do you pay? I am hoping to find someone to perform vacation coverage, and want to be fair in the salary that we offer. Thanks, Bonnie Whitaker Lab Manager Brown & Associates Medical Laboratories 8076 El Rio Houston, Texas 77054 713-741-6677 From rjbuesa <@t> yahoo.com Wed May 30 11:19:30 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 30 11:19:35 2007 Subject: [Histonet] PRN Grossing salary In-Reply-To: <002001c7a2d0$63e5cb90$3601a8c0@brownpathology.net> Message-ID: <762409.31178.qm@web61223.mail.yahoo.com> Unfortunately salaries in our field vary wildly, not just between areas in the country, but even between labs in a single county. This is a "supply-demand" issue; offer a salary conmensurate to your needs and comparable to other salaries you are now paying, and let the applicant decide. Specially think on those now working in your lab and what they are being paid. The least you want is to be confronted by a colleague being paid less than the "new-comer", it would not be fair. Ren? J. Bonnie Whitaker wrote: Hi Everyone, I would like to ask if anyone is using (eligible) techs and/or PA's to gross small specimens on a PRN basis, and if so, what hourly rate do you pay? I am hoping to find someone to perform vacation coverage, and want to be fair in the salary that we offer. Thanks, Bonnie Whitaker Lab Manager Brown & Associates Medical Laboratories 8076 El Rio Houston, Texas 77054 713-741-6677 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Got a little couch potato? Check out fun summer activities for kids. From HornHV <@t> archildrens.org Wed May 30 12:24:22 2007 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed May 30 12:24:36 2007 Subject: [Histonet] PRN Grossing salary In-Reply-To: <762409.31178.qm@web61223.mail.yahoo.com> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB7162@EMAIL.archildrens.org> Our zero based employees get a higher salary than our FTE's because they get no benefits. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, May 30, 2007 11:20 AM To: Bonnie Whitaker; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] PRN Grossing salary Unfortunately salaries in our field vary wildly, not just between areas in the country, but even between labs in a single county. This is a "supply-demand" issue; offer a salary conmensurate to your needs and comparable to other salaries you are now paying, and let the applicant decide. Specially think on those now working in your lab and what they are being paid. The least you want is to be confronted by a colleague being paid less than the "new-comer", it would not be fair. Ren? J. Bonnie Whitaker wrote: Hi Everyone, I would like to ask if anyone is using (eligible) techs and/or PA's to gross small specimens on a PRN basis, and if so, what hourly rate do you pay? I am hoping to find someone to perform vacation coverage, and want to be fair in the salary that we offer. Thanks, Bonnie Whitaker Lab Manager Brown & Associates Medical Laboratories 8076 El Rio Houston, Texas 77054 713-741-6677 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Got a little couch potato? Check out fun summer activities for kids. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From sbledsoe <@t> iupui.edu Wed May 30 13:42:17 2007 From: sbledsoe <@t> iupui.edu (Sharon Bledsoe) Date: Wed May 30 13:42:24 2007 Subject: [Histonet] (no subject) Message-ID: Is there a histological stain for cystine that will stain crystals? I need look for very small crystals in tissue. Thanks, Sharon From tim.morken <@t> thermofisher.com Wed May 30 15:49:01 2007 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Wed May 30 15:49:28 2007 Subject: [Histonet] FW: Positions at ThermoFisher Scientific Message-ID: <6BFF6D137DF6BC43B33891BA96E83B19714930@PGHCR-EXMB-VS-1.na.fshrnet.com> We have two openings at ThermoFisher Scientific, Lab Vision Products in Fremont, CA. First is a lab technologist position in the QC lab. This involves running IHC test for antibody, detection systems and ancillary IHC reagents. This person will also be involved in product development on some projects. The ideal person will also be proficient at reviewing IHC slides for QC release. Second is a technical support position. This is an in-house postion answering customer questions and resolving customer complaints. Part of the job is lab work testing reagents for reported complaints or problems. The position may also involve occasional travel to customer sites anywhere in the US (about 10-15% of the total time). Ideal candidates for each will be HT or HTL and QIHC registered. Salary for each is commensurate with experience and competitive for the San Fransisco Bay Area. ThermoFisher Scientific, Lab Vision Products, provides a full line of IHC instrumentation, antibodies and IHC reagents for research and diagnostic laboratories. Please see our website at: www.labvision.com If interested please forward resumes to me at tim.morken@thermofisher.com Tim Morken Technical Support Manager Anatomical Pathology ThermoFisher Scientific 47777 Warm Springs Blvd. Fremont, CA 94539 USA tel: 01-510-991-2840 fax: 01 510-991-2826 Tim.Morken@thermofisher.com web: www.labvision.com www.thermofisher.com The World Leader in Serving Science From SDrew <@t> uwhealth.org Wed May 30 15:58:56 2007 From: SDrew <@t> uwhealth.org (Drew Sally A.) Date: Wed May 30 15:59:01 2007 Subject: [Histonet] PAC-theta Message-ID: Is there anyone out there doing PKC-theta? We have a pathologist looking to have this done on a rare basis... Thank you! Sally Ann Drew, MT(ASCP) IHC/ISH Laboratory University of Wisconsin Hosp. & Clinics Madison, WI 53792 (608)265-6596 From gcallis <@t> montana.edu Wed May 30 16:35:51 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed May 30 16:35:39 2007 Subject: [Histonet] Human protein atlas, a website to behold! Message-ID: <6.0.0.22.1.20070530152750.01b4a820@gemini.msu.montana.edu> Dear Histonetters, A colleague just provided an incredible website for those looking information about antibodies to human proteins. It is the Human Protein Atlas, and you simply type in the protein you want to detect. If you click on Antibody ID, you will bring up a chart with tissue list, and if immunostaining is strong to none. You can select a specific tissue. It looks like a microarray stained tissue, but you will know if the antibody is going to work for your needs. You can access all kinds of detailed information about the protein, sequencing, etc and publications. Anything to make our work easier. Enjoy! Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From Eric.Hoy <@t> UTSouthwestern.edu Wed May 30 16:56:47 2007 From: Eric.Hoy <@t> UTSouthwestern.edu (Eric Hoy) Date: Wed May 30 16:56:55 2007 Subject: [Histonet] Human protein atlas, a website to behold! In-Reply-To: <6.0.0.22.1.20070530152750.01b4a820@gemini.msu.montana.edu> Message-ID: On 5/30/07 4:35 PM, "Gayle Callis" wrote: > Dear Histonetters, > > A colleague just provided an incredible website for those looking > information about antibodies to human proteins. It is the Human Protein > Atlas, and you simply type in the protein you want to detect. If you click > on Antibody ID, you will bring up a chart with tissue list, and if > immunostaining is strong to none. You can select a specific tissue. It > looks like a microarray stained tissue, but you will know if the antibody > is going to work for your needs. You can access all kinds of detailed > information about the protein, sequencing, etc and publications. > > Anything to make our work easier. > > Enjoy! Gayle, Thank you for pointing out this website. The URL is: http://www.proteinatlas.org/ There is a wealth of information there, even which chromosome carries the gene for the protein. Thank you! Eric =================================================== Eric S. Hoy, Ph.D., SI(ASCP) Clinical Associate Professor Department of Medical Laboratory Sciences The University of Texas Southwestern Medical Center Dallas, Texas Email: Eric.Hoy@UTSouthwestern.edu =================================================== From gcallis <@t> montana.edu Wed May 30 16:59:41 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed May 30 16:59:28 2007 Subject: [Histonet] Link to Human protein atlas, a website to behold! In-Reply-To: References: <6.0.0.22.1.20070530152750.01b4a820@gemini.msu.montana.edu> Message-ID: <6.0.0.22.1.20070530155641.01b66db0@gemini.msu.montana.edu> Sorry, the link is www.proteinatlas.org for the Human Protein Atlas. Gayle Callis At 03:44 PM 5/30/2007, you wrote: >Hi Gayle, > >What is the link?! Sounds cooooooool. > >Thanks in advance, >Andrea > > > >>Dear Histonetters, >> >>A colleague just provided an incredible website for those looking >>information about antibodies to human proteins. It is the Human Protein >>Atlas, and you simply type in the protein you want to detect. If you >>click on Antibody ID, you will bring up a chart with tissue list, and if >>immunostaining is strong to none. You can select a specific tissue. It >>looks like a microarray stained tissue, but you will know if the antibody >>is going to work for your needs. You can access all kinds of detailed >>information about the protein, sequencing, etc and publications. From es144131 <@t> bcm.tmc.edu Wed May 30 17:43:09 2007 From: es144131 <@t> bcm.tmc.edu (Stephens, Elizabeth Humes) Date: Wed May 30 17:43:14 2007 Subject: [Histonet] Picrosirius Red in Valves Message-ID: <10C24F7C4D05EB45B5F0E1B3978978490131F3A5@BCMEVS7.ad.bcm.edu> I'm trying to stain for collagen I in porcine heart valves, but have had trouble getting good staining with various antibodies. Do you know if collagen I can be distinguished from other collagens using picrosirius red with polarized light? I do have a nice Collagen III Antibody (which is the other major collagen in heart valves), so I could potentially approximate the collagen I as the difference bw picrosirius red and collagen III staining. (My problem with collagen I antibodies is that I seem to get strong staining around the collagen dense valve annulus core, but light staining in the areas that stain yellow by Movat. The antibody does bind C-propeptide of collagen I, so I don't know if these precursors would be present around the annulus core and not within the core?) Thank you!! From kwuny <@t> email.cs.nsw.gov.au Wed May 30 18:53:09 2007 From: kwuny <@t> email.cs.nsw.gov.au (Young Kwun) Date: Wed May 30 18:50:22 2007 Subject: [Histonet] Help with MDM2 Protein Immunostaining Message-ID: <20070531094956.SM01256@crgcsls814> Dear Colleagues, Does anyone in Histoland use MDM2 Protein staining for some soft tissue sarcomas successfully? I tried Lab Vision's polyclonal antibody (1/100 dil) and Novocastra's MDM2 (clone 1B10, 1/150 dil) with Decloaker HIER and BondMax Autostainer. However, my pathologist is not happy with the staining results, mainly because of some non-specific stains. Any help would be appreciated. Thank you. Young Kwun Senior Hospital Scientist Dept of Anatomical Pathology Concord Hospital Concord NSW 2139 Australia Phone:61-2-9767 6075 Fax:61-2-9767 8427 Email:kwuny@email.cs.nsw.gov.au From SDrew <@t> uwhealth.org Thu May 31 07:30:25 2007 From: SDrew <@t> uwhealth.org (Drew Sally A.) Date: Thu May 31 07:30:31 2007 Subject: [Histonet] Oops, I meant PKC-theta In-Reply-To: Message-ID: Sally Ann Drew, MT(ASCP) IHC/ISH Laboratory University of Wisconsin Hosp. & Clinics Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Drew Sally A. Sent: Wednesday, May 30, 2007 3:59 PM To: Histonet Subject: [Histonet] PAC-theta Is there anyone out there doing PKC-theta? We have a pathologist looking to have this done on a rare basis... Thank you! Sally Ann Drew, MT(ASCP) IHC/ISH Laboratory University of Wisconsin Hosp. & Clinics Madison, WI 53792 (608)265-6596 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mgdelaware <@t> comcast.net Thu May 31 09:33:12 2007 From: mgdelaware <@t> comcast.net (marian powers) Date: Thu May 31 09:33:23 2007 Subject: [Histonet] Manual HER2 FISH Message-ID: Hello, I was wondering if anyone has a good manual Her2 FISH protocol. We have switched to a immunostainer that does not offer FISH. I have tried to use our left over Ventana kit but keep losing the tissue. Thanks in advance, Marian Powers From gcallis <@t> montana.edu Thu May 31 09:42:28 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu May 31 09:42:12 2007 Subject: [Histonet] Human protein atlas, a website to behold! In-Reply-To: References: <6.0.0.22.1.20070530152750.01b4a820@gemini.msu.montana.edu> Message-ID: <6.0.0.22.1.20070531083826.01b38978@gemini.msu.montana.edu> I wouldn't be surprised that the list is NOT complete. It certainly must be an ongoing project to add more to the list as information becomes available. At 05:56 PM 5/30/2007, you wrote: >Unfortunately, I just searched for the numerous proteins we work on here >in this lab (all of which we have working and robust immunostaining >protocols for) and none are in there, so I wonder how complete the atlas >is ...... hmmm ... > > > > >>Dear Histonetters, >> >>A colleague just provided an incredible website for those looking >>information about antibodies to human proteins. It is the Human Protein >>Atlas, and you simply type in the protein you want to detect. If you >>click on Antibody ID, you will bring up a chart with tissue list, and if >>immunostaining is strong to none. You can select a specific tissue. It >>looks like a microarray stained tissue, but you will know if the antibody >>is going to work for your needs. You can access all kinds of detailed >>information about the protein, sequencing, etc and publications. >> >>Gayle Callis >>MT,HT,HTL(ASCP) >>Research Histopathology Supervisor >>Veterinary Molecular Biology >>Montana State University - Bozeman >>PO Box 173610 >Bozeman MT 59717-3610 > >-- From lancebeard01 <@t> yahoo.com Thu May 31 09:45:02 2007 From: lancebeard01 <@t> yahoo.com (Lance Beard) Date: Thu May 31 09:45:09 2007 Subject: [Histonet] Histology position available Message-ID: <002801c7a392$45bd1200$6701a8c0@Histopath.net> Our private histology lab is accepting applications for a Histotechnician for its Corpus Christi location. Excellent salary and benefits including health for employee and family. Sign-on bonus. Fax your resume to: 361.992.3847 ________________________________________________ Lance Beard Administrator Pathology Associates of Corpus Christi, LLP HistoPath, Inc. 3853 S. Alameda Corpus Christi, Texas 78411 361/992-4211 361/992-3847 (fax) From POWELL_SA <@t> Mercer.edu Thu May 31 10:37:28 2007 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Thu May 31 10:38:51 2007 Subject: [Histonet] Mealworm larvae Message-ID: <01MH8G5SJZUQ0006LM@Macon2.Mercer.edu> Hi Guys, I need help with this one. One of my investigators is requesting histology on mealworm larvae, which eventually turn into mealworm beetles. Anyone who has worked with or has information on processing these lovely little creatures in the larvae stage, please share your processing procedure with me. Paraffin or plastic. Thanks in advance. Shirley Powell From settembr <@t> umdnj.edu Thu May 31 10:39:41 2007 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Thu May 31 10:40:35 2007 Subject: [Histonet] Help with MDM2 Protein Immunostaining Message-ID: Hello Young Kwun, I use MDM2 from Dako cat.# M7146 It is IVD. My pathologist seems satisfied with this. I use Dako's Target Retreival Solution in a steamer for 40 minutes. I use it at 1:40. Good Luck, Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Young Kwun 05/30/07 7:53 PM >>> Dear Colleagues, Does anyone in Histoland use MDM2 Protein staining for some soft tissue sarcomas successfully? I tried Lab Vision's polyclonal antibody (1/100 dil) and Novocastra's MDM2 (clone 1B10, 1/150 dil) with Decloaker HIER and BondMax Autostainer. However, my pathologist is not happy with the staining results, mainly because of some non-specific stains. Any help would be appreciated. Thank you. Young Kwun Senior Hospital Scientist Dept of Anatomical Pathology Concord Hospital Concord NSW 2139 Australia Phone:61-2-9767 6075 Fax:61-2-9767 8427 Email:kwuny@email.cs.nsw.gov.au _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Thu May 31 10:43:32 2007 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Thu May 31 10:42:03 2007 Subject: [Histonet] Picrosirius Red in Valves In-Reply-To: <10C24F7C4D05EB45B5F0E1B3978978490131F3A5@BCMEVS7.ad.bcm.edu> Message-ID: <200705311541.l4VFflAd069619@pro12.abac.com> I think your best bet for collagen antibodies is from the U of Iowa Hybridoma Bank, they are all mouse monoclonal, not sure if they cross react with porcine but they can tell you at the bank and put you in contact with the investigator who developed the clone. I have done a lot of picro/sirius staining using polarized light but I do not think you can distinguish collagen types, we used it to measure collagen bundles but not types of collagen. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephens, Elizabeth Humes Sent: Wednesday, May 30, 2007 4:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Picrosirius Red in Valves I'm trying to stain for collagen I in porcine heart valves, but have had trouble getting good staining with various antibodies. Do you know if collagen I can be distinguished from other collagens using picrosirius red with polarized light? I do have a nice Collagen III Antibody (which is the other major collagen in heart valves), so I could potentially approximate the collagen I as the difference bw picrosirius red and collagen III staining. (My problem with collagen I antibodies is that I seem to get strong staining around the collagen dense valve annulus core, but light staining in the areas that stain yellow by Movat. The antibody does bind C-propeptide of collagen I, so I don't know if these precursors would be present around the annulus core and not within the core?) Thank you!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mber <@t> vt.edu Thu May 31 11:38:02 2007 From: mber <@t> vt.edu (mber@vt.edu) Date: Thu May 31 11:38:09 2007 Subject: [Histonet] H&E staining protocol (manual) for chicken guts Message-ID: <1180629482.465ef9ea648a8@webmail.vt.edu> Hi, Does anyone have an H&E staining protocol for chicken gut including type and brand of H&E?Thanks. Meg Berger Histologist Animal and Poultry Sciences VA Tech From kmilne <@t> bccancer.bc.ca Thu May 31 11:46:07 2007 From: kmilne <@t> bccancer.bc.ca (Milne, Katy) Date: Thu May 31 11:46:16 2007 Subject: [Histonet] DIG ISH on Ventana Message-ID: <07979E76B0869D4E8C9FE4AA9FC065780240D628@srvex03.phsabc.ehcnet.ca> Hi guys, I need to look for interferon-g in FFPE mouse and human tissue, I am ordering some DIG probes and am planning on using our Ventana Discovery system. I am planning on bringing in an Amp-MAP kit for TSA and I notice that the kit starts with a strep-HRP step which would tell me that I need a biotinylated anti-DIG Ab; however in a few papers I've seen people using an HRP-conjugated anti-DIG Ab for tyramide amplification, I'm guessing perhaps they're somehow eliminating the first step of the kit or doing something slightly different. Anyway, I'm just wondering if anyone has experience with this and if I need a biotinylated or an HRP conjugated anti-DIG Ab for use with the AmpMap kit. Also, which detection kits does everyone use, we have RedMap and DabMap in house. Is BlueMap necessary? I'm trying to get these reagents in ASAP but I want to make sure I have the right stuff so am looking for someone with experience for guidance! Thanks in advance, Katy Milne Deeley Research Centre BC Cancer Agency From dpahisto <@t> yahoo.com Thu May 31 12:13:18 2007 From: dpahisto <@t> yahoo.com (Cindy DuBois) Date: Thu May 31 12:13:21 2007 Subject: [Histonet] Hazardous waste Hauler? Message-ID: <496946.66956.qm@web33415.mail.mud.yahoo.com> If you work in California can you tell me who you use for your hazardous waste hauler? We were using Romic, but it's plant is being closed. Thank you, Cindy DuBois Integrated Pathology Stockton, CA --------------------------------- Pinpoint customers who are looking for what you sell. From relia1 <@t> earthlink.net Thu May 31 13:13:39 2007 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu May 31 13:13:44 2007 Subject: [Histonet] Histo Tech needed in MD. Can you help? Message-ID: Hi Histonetters, I am currently working with a client in Central Maryland that is in need of a histo tech. This is a permanent full time dayshift position. The client offers a great hospital environment, a great crew to work with and excellent benefits and salary. My question is do you know of anyone who might be interested in this position? If you think you or someone you know might be interested please contact me. I can be reached at 866-607-3542 or relia1@earthlink.net Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net From Maxim_71 <@t> mail.ru Thu May 31 14:05:21 2007 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Thu May 31 14:23:41 2007 Subject: [Histonet] processing fatty tissue Message-ID: <1783802675.20070531230521@mail.ru> I completely agree with Rene. When we processed breasts (and other tissues) with isopropanol+mineral oil that we ceased to feel the difficulties with our samples. We have manually processing. More thanks to Dr. McCormick for cassettes! Sincerely, Maxim Peshkov Russia Taganrog "Rene J Buesa" wrote: Date: Tue, 29 May 2007 06:22:59 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] processing fatty tissue To: "Orr, Rebecca" , histonet@lists.utsouthwestern.edu Cc: Gervaip@aol.com 1- thin slices 2- well fixed after the slices are prepared (at least 8 h) 3- use fresh alcohols for dehydration 4- extend somewaht (20%) the clearing steps time (time x 1.2), and 5- if possible use a 60?C paraffin, OR process with ethanol followed by a mixture of ethanol+propanol+mineral oil, and breats will cut "like butter". Ren? J. "Orr, Rebecca" wrote: Message: 11 Date: Fri, 25 May 2007 22:57:59 EDT From: Gervaip@aol.com Subject: [Histonet] processing of fatty tissue samples To: histonet@pathology.swmed.edu Hi, what is everyone doing in histo land when it comes to processing breast tissue? Pearl Hi Pearl, We now keep a processor dedicated to (fatty) breast tissue. We do also run fatty tissue with this same process. You can check out the CAP guidelines for Breast tissue that may help with your process. We first had to decide if our fatty tissues were either partially fixed, partially cleared or a portion of both. Each time we had unacceptable tissue we would troubleshoot back the whole way to the OR. Our problem ended up as a combination of our Gross Staff (residents and PA's) submitting thicker pieces, cramming cassettes and not enough clearing time. We decided our fixation time was appropriate and well within the CAP guidelines. So we added more time in our 100% alcohols (you'll need to decide if you should add more time or change reagents more frequently or add an additional container of 100%) We also adjusted the processing schedule and added a third station of xylene. Our VIP has 4 paraffin baths and we use all of them with varied times. We now have excellent results with this set up. ~A simple reminder every hour on the hour to the Gross Room to "keep the tissue thin" helps everything run smoothly! (LOL) It is a great help for us to have the capability to separate our tissues in this manner with several processors. Becky Orr CLA,HT(ASCP)QIHC Assistant Manager, Anatomic Pathology Evanston Northwestern Healthcare 847-570-2763 From asachau <@t> titanmed.com Thu May 31 14:27:15 2007 From: asachau <@t> titanmed.com (April Sachau) Date: Thu May 31 14:28:19 2007 Subject: [Histonet] (no subject) In-Reply-To: <1783802675.20070531230521@mail.ru> Message-ID: <7E3ACD48BA6E26408F3188FBF08693F7943818@titansbs1.corp.titanmed.com> How much does the average Mohs technician make for an hourly wage? April Sachau Titan Medical Group Staff Supervisor Phone (866) 332-9600 Ext. 1023 Fax (402) 332-5181 asachau@titanmed.com see us on the web at www.titanmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maxim Peshkov Sent: Thursday, May 31, 2007 2:05 PM To: ROrr@enh.org Cc: histonet@lists.utsouthwestern.edu; Gervaip@aol.com Subject: Re: [Histonet] processing fatty tissue I completely agree with Rene. When we processed breasts (and other tissues) with isopropanol+mineral oil that we ceased to feel the difficulties with our samples. We have manually processing. More thanks to Dr. McCormick for cassettes! Sincerely, Maxim Peshkov Russia Taganrog "Rene J Buesa" wrote: Date: Tue, 29 May 2007 06:22:59 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] processing fatty tissue To: "Orr, Rebecca" , histonet@lists.utsouthwestern.edu Cc: Gervaip@aol.com 1- thin slices 2- well fixed after the slices are prepared (at least 8 h) 3- use fresh alcohols for dehydration 4- extend somewaht (20%) the clearing steps time (time x 1.2), and 5- if possible use a 60?C paraffin, OR process with ethanol followed by a mixture of ethanol+propanol+mineral oil, and breats will cut "like butter". Ren? J. "Orr, Rebecca" wrote: Message: 11 Date: Fri, 25 May 2007 22:57:59 EDT From: Gervaip@aol.com Subject: [Histonet] processing of fatty tissue samples To: histonet@pathology.swmed.edu Hi, what is everyone doing in histo land when it comes to processing breast tissue? Pearl Hi Pearl, We now keep a processor dedicated to (fatty) breast tissue. We do also run fatty tissue with this same process. You can check out the CAP guidelines for Breast tissue that may help with your process. We first had to decide if our fatty tissues were either partially fixed, partially cleared or a portion of both. Each time we had unacceptable tissue we would troubleshoot back the whole way to the OR. Our problem ended up as a combination of our Gross Staff (residents and PA's) submitting thicker pieces, cramming cassettes and not enough clearing time. We decided our fixation time was appropriate and well within the CAP guidelines. So we added more time in our 100% alcohols (you'll need to decide if you should add more time or change reagents more frequently or add an additional container of 100%) We also adjusted the processing schedule and added a third station of xylene. Our VIP has 4 paraffin baths and we use all of them with varied times. We now have excellent results with this set up. ~A simple reminder every hour on the hour to the Gross Room to "keep the tissue thin" helps everything run smoothly! (LOL) It is a great help for us to have the capability to separate our tissues in this manner with several processors. Becky Orr CLA,HT(ASCP)QIHC Assistant Manager, Anatomic Pathology Evanston Northwestern Healthcare 847-570-2763 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mber <@t> vt.edu Thu May 31 14:45:18 2007 From: mber <@t> vt.edu (mber@vt.edu) Date: Thu May 31 14:45:23 2007 Subject: [Histonet] Chicken gut protocol Message-ID: <1180640717.465f25ce001e3@webmail.vt.edu> Thanks all for the help. Meg From POWELL_SA <@t> Mercer.edu Thu May 31 14:56:53 2007 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Thu May 31 14:58:32 2007 Subject: [Histonet] FW: Mealworm larvae Message-ID: <01MH8P7FKTK60008EQ@Macon2.Mercer.edu> Okay there must be more people working with chicken gut than with mealworms. I still need help, the project is coming up soon, mealworms coming in next week. Help with processing is needed. Thanks, Shirley -----Original Message----- From: Shirley Powell [mailto:powell_sa@mercer.edu] Sent: Thursday, May 31, 2007 11:37 AM To: 'histonet@lists.utsouthwestern.edu' Subject: Mealworm larvae Hi Guys, I need help with this one. One of my investigators is requesting histology on mealworm larvae, which eventually turn into mealworm beetles. Anyone who has worked with or has information on processing these lovely little creatures in the larvae stage, please share your processing procedure with me. Paraffin or plastic. Thanks in advance. Shirley Powell From HoustonR <@t> chi.osu.edu Thu May 31 14:58:32 2007 From: HoustonR <@t> chi.osu.edu (Houston, Ronald) Date: Thu May 31 14:58:58 2007 Subject: [Histonet] Mollier's Quadruple stain Message-ID: <979FF5962E234F45B06CF0DB7C1AABB20FEC1E06@chi2k3ms01.columbuschildrens.net> This is a new one on me; one of our pathologists is asking about the possibility of doing a Mollier's Quadruple stain. I have never heard of it, although I believe it is an all encompassing connective tissue stain (at least that's what he calls it). Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Columbus Children's Hospital 700 Children's Drive Columbus, OH 43205 ----------------------------------------- Confidentiality Notice: This e-mail message, from Children's Hospital, Columbus, Ohio, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes pursuant to Children's Hospital's confidentiality policies. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From victor <@t> pathology.washington.edu Thu May 31 15:08:12 2007 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Thu May 31 15:08:19 2007 Subject: [Histonet] Mollier's Quadruple stain In-Reply-To: <979FF5962E234F45B06CF0DB7C1AABB20FEC1E06@chi2k3ms01.columbuschildrens.net> References: <979FF5962E234F45B06CF0DB7C1AABB20FEC1E06@chi2k3ms01.columbuschildrens.net> Message-ID: <465F2B2C.2040507@pathology.washington.edu> I saw a reference to it as Gridley's. Houston, Ronald wrote: > This is a new one on me; one of our pathologists is asking about the > possibility of doing a Mollier's Quadruple stain. I have never heard of > it, although I believe it is an all encompassing connective tissue stain > (at least that's what he calls it). > > > > > > Ronnie Houston, MS, HT(ASCP)QIHC > > Anatomic Pathology Manager > > Columbus Children's Hospital > > 700 Children's Drive > > Columbus, OH 43205 > > > > > > ----------------------------------------- > Confidentiality Notice: This e-mail message, from Children's > Hospital, Columbus, Ohio, including any attachments, is for the > sole use of the intended recipient(s) and may contain confidential > and privileged information. The recipient is responsible to > maintain the confidentiality of this information and to use the > information only for authorized purposes pursuant to Children's > Hospital's confidentiality policies. If you are not the intended > recipient (or authorized to receive information for the intended > recipient), you are hereby notified that any review, use, > disclosure, distribution, copying, printing, or action taken in > reliance on the contents of this e-mail is strictly prohibited. If > you have received this communication in error, please notify us > immediately by reply e-mail and destroy all copies of the original > message. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From rjbuesa <@t> yahoo.com Thu May 31 15:14:25 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu May 31 15:14:28 2007 Subject: [Histonet] Mollier's Quadruple stain In-Reply-To: <979FF5962E234F45B06CF0DB7C1AABB20FEC1E06@chi2k3ms01.columbuschildrens.net> Message-ID: <292348.79365.qm@web61215.mail.yahoo.com> Ronald: Mollier published this procedure in the "Journal for Scientific microscopy and microtechnike (Leipzig) in German vol.55 pp 472 in 1938. It uses the cartilage stain by Unna-Tanzer (1896) and the Weigert hematoxylin (1904 formula), among other components. The results are nucle??deep blue; cytoplasm??purple; elastic fibres??black; collagen??gree and erythrocytes ??scarlet. Ren?? J. "Houston, Ronald" wrote: This is a new one on me; one of our pathologists is asking about the possibility of doing a Mollier's Quadruple stain. I have never heard of it, although I believe it is an all encompassing connective tissue stain (at least that's what he calls it). Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Columbus Children's Hospital 700 Children's Drive Columbus, OH 43205 ----------------------------------------- Confidentiality Notice: This e-mail message, from Children's Hospital, Columbus, Ohio, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes pursuant to Children's Hospital's confidentiality policies. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your own web address. Have a HUGE year through Yahoo! Small Business. From LuckG <@t> empirehealth.org Thu May 31 15:22:04 2007 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Thu May 31 15:22:12 2007 Subject: [Histonet] FW: Job Opening Message-ID: <6BB8BC4519AAB844B174FC739A679BBCCEFDDC@IRMEXCH01.irm.inhs.org> Hello all, We have an histology position open for an HT or HTL certifed tech. Routine histology duties (embedding, cutting and special stains. IHC experience in addition preferred. This position is in a 300 bed community hospital in Spokane, WA. Below I have placed three weblinks for those interested so that they can go on the internet to look at our facility, corporate organization and the community and surrounding region. A great place to work and live. Please contact me directly. Thanks, Greg www.empirehealth.org www.deaconessmc.org www.visitspokane.com Greg Luck Anatomic Path Spvr Deaconess Med Cntr 800 W. 5th Ave Spokane, WA 99204 Offc 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org From dw18 <@t> uchicago.edu Thu May 31 15:55:12 2007 From: dw18 <@t> uchicago.edu (David A. Wright) Date: Thu May 31 15:55:17 2007 Subject: [Histonet] Re: Mealworm larvae Message-ID: <20070531155512.ANR03476@m4500-03.uchicago.edu> Hello Shirley & Histonet I used to do a lot of microscopy with different insects. Here are some general suggestions, mostly for the early part of tissue isolation - if that's the challenge, but it would help to know: a) which species of mealworm? (Because they vary in size and that would obviously affect the choice of method) b) which tissue? Mealworms might include the tiny flour beetles, Tribolium, since they are used for genetic research, the midsized common petstore mealworm, Tenebrio, and the giant Super Mealworm, Zophobas (also petstores). Insects are easy to take apart since the cuticle holds stuff together after cutting, different organs are largely separate, and there is no vasculature beyond a simple tubular "heart" (along the back). There are white spidery tubes of air (tracheae) everywhere but these can be ignored. If you want to you can dissect in a general Insect Ringer solution (e.g. NaCl 9.1 g/L, KCl 0.52 g/L, CaCl2.2H2O, 1.2 g/L MgCl2.6H2O .8 g/L). Cells will stay alive for quite a while. Pin the insect out on wax or Sylgard, using "Minuten" pins (from e.g. Carolina Biological & used for mounting very tiny insects in museum collections) in a drop of ringer. Before you try cutting them up (or immobilizing below), a simple way to narcotize them is to drown them in degassed water until they stop wriggling (10-15'?). Use a little vial or bottle with a slight lip, sideways in a tray of water. Duck the larva under the lip and then if it bobs upward it can't come to the surface. Try to eliminate trapped bubbles (I use a paintbrush). Chilling is also effective. For epidermis (technically hypodermis), simply make whole mounts of the cuticle (if it is transparent enough) with the attached cells - they form a monolayer and no sectioning is necessary. Chop out the part of interest with a single edge razor blade on some soft plastic (polyethylene of "nalgene' softness). Rip this part away from the carcass with forceps and immediately plunge into a vial of fixative. I used fresh Carnoy for haematoxylin staining. You don't even need any saline, though you might have to trim off odd bits of fat body and trachea after fixation (which is of 'tissue culture" rapidity since it's a monolayer). If you want to section (and infiltrate) I suggest you immobilize the insect (e.g. with little strips of modelling clay on a slab of the same on top of a bottle cap or petri lid that you can manipulate easily). Then inflate the body by rapidly injecting your chosen fix. They stretch a bit and the cuticle usually stops them from bursting. You could use a regular hypodermic & fine needle in the larger guys. In smaller ones, draw out the tip of a Pasteur pipette in a flame, break off the tip to a sharp point of suitable diameter and use that to inject the fixative. The intersegmental cuticle (between the hard rings) is a good place to impale. You can also cut off a leg (tiny in larvae), or even the head, and inject through the hole so made - just make sure the needle is nearly the same diameter as the hole (the taper on a drawn pipette makes this easier). Once the body has fixed a bit the increased firmness makes it easier to cut off the ends of the larvae (razor or fine scissors) and process the middle bit, a hollow tube, conventionally. You can also slit the tube longitudinally with fine scissors. The outer layer of insect cuticle does not like being wax embedded (it separates) - there was a recent brief thread started by Anthony Gatt on how to improve things. Resin is easier, since that's ok with your final goal. Hope this helps some -David --------------- David A. Wright, PhD Section of Neurosurgery University of Chicago ===================================== Histonet Digest, Vol 42, Issue 44 Message: 14 Date: Thu, 31 May 2007 11:37:28 -0400 From: Shirley Powell Subject: [Histonet] Mealworm larvae To: histonet@lists.utsouthwestern.edu Hi Guys, I need help with this one. One of my investigators is requesting histology on mealworm larvae, which eventually turn into mealworm beetles. Anyone who has worked with or has information on processing these lovely little creatures in the larvae stage, please share your processing procedure with me. Paraffin or plastic. Thanks in advance. Shirley Powell From dellav <@t> musc.edu Thu May 31 17:06:22 2007 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Thu May 31 17:06:22 2007 Subject: [Histonet] Mohs tech salaries In-Reply-To: <7E3ACD48BA6E26408F3188FBF08693F7943818@titansbs1.corp.titanmed.com> References: <1783802675.20070531230521@mail.ru> <7E3ACD48BA6E26408F3188FBF08693F7943818@titansbs1.corp.titanmed.com> Message-ID: <7F6B678A32B0564196138E6B3101996A52D169@EVS1.clinlan.local> The Mohs society conducts regular salary surveys. Go to http://mohscollege.org/ASMH.htm and contact the society's leadership for the information you are seeking Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of April Sachau Sent: Thursday, May 31, 2007 3:27 PM To: Maxim Peshkov; ROrr@enh.org Cc: histonet@lists.utsouthwestern.edu; Gervaip@aol.com Subject: [Histonet] (no subject) How much does the average Mohs technician make for an hourly wage? April Sachau Titan Medical Group Staff Supervisor Phone (866) 332-9600 Ext. 1023 Fax (402) 332-5181 asachau@titanmed.com see us on the web at www.titanmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maxim Peshkov Sent: Thursday, May 31, 2007 2:05 PM To: ROrr@enh.org Cc: histonet@lists.utsouthwestern.edu; Gervaip@aol.com Subject: Re: [Histonet] processing fatty tissue I completely agree with Rene. When we processed breasts (and other tissues) with isopropanol+mineral oil that we ceased to feel the difficulties with our samples. We have manually processing. More thanks to Dr. McCormick for cassettes! Sincerely, Maxim Peshkov Russia Taganrog "Rene J Buesa" wrote: Date: Tue, 29 May 2007 06:22:59 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] processing fatty tissue To: "Orr, Rebecca" , histonet@lists.utsouthwestern.edu Cc: Gervaip@aol.com 1- thin slices 2- well fixed after the slices are prepared (at least 8 h) 3- use fresh alcohols for dehydration 4- extend somewaht (20%) the clearing steps time (time x 1.2), and 5- if possible use a 60?C paraffin, OR process with ethanol followed by a mixture of ethanol+propanol+mineral oil, and breats will cut "like butter". Ren? J. "Orr, Rebecca" wrote: Message: 11 Date: Fri, 25 May 2007 22:57:59 EDT From: Gervaip@aol.com Subject: [Histonet] processing of fatty tissue samples To: histonet@pathology.swmed.edu Hi, what is everyone doing in histo land when it comes to processing breast tissue? Pearl Hi Pearl, We now keep a processor dedicated to (fatty) breast tissue. We do also run fatty tissue with this same process. You can check out the CAP guidelines for Breast tissue that may help with your process. We first had to decide if our fatty tissues were either partially fixed, partially cleared or a portion of both. Each time we had unacceptable tissue we would troubleshoot back the whole way to the OR. Our problem ended up as a combination of our Gross Staff (residents and PA's) submitting thicker pieces, cramming cassettes and not enough clearing time. We decided our fixation time was appropriate and well within the CAP guidelines. So we added more time in our 100% alcohols (you'll need to decide if you should add more time or change reagents more frequently or add an additional container of 100%) We also adjusted the processing schedule and added a third station of xylene. Our VIP has 4 paraffin baths and we use all of them with varied times. We now have excellent results with this set up. ~A simple reminder every hour on the hour to the Gross Room to "keep the tissue thin" helps everything run smoothly! (LOL) It is a great help for us to have the capability to separate our tissues in this manner with several processors. Becky Orr CLA,HT(ASCP)QIHC Assistant Manager, Anatomic Pathology Evanston Northwestern Healthcare 847-570-2763 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From llewllew <@t> shaw.ca Thu May 31 17:19:18 2007 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Thu May 31 17:19:33 2007 Subject: [Histonet] Mollier's Quadruple stain References: <979FF5962E234F45B06CF0DB7C1AABB20FEC1E06@chi2k3ms01.columbuschildrens.net> Message-ID: <001701c7a3d1$bb45bab0$ff144246@yourlk4rlmsu> Details are at: http://stainsfile.info/StainsFile/stain/conektv/tri_mollier.htm Bryan Llewellyn ----- Original Message ----- From: "Houston, Ronald" To: Sent: Thursday, May 31, 2007 12:58 PM Subject: [Histonet] Mollier's Quadruple stain This is a new one on me; one of our pathologists is asking about the possibility of doing a Mollier's Quadruple stain. I have never heard of it, although I believe it is an all encompassing connective tissue stain (at least that's what he calls it). Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Columbus Children's Hospital 700 Children's Drive Columbus, OH 43205 ----------------------------------------- Confidentiality Notice: This e-mail message, from Children's Hospital, Columbus, Ohio, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes pursuant to Children's Hospital's confidentiality policies. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Thu May 31 17:27:47 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu May 31 17:27:53 2007 Subject: [Histonet] FW: Mealworm larvae In-Reply-To: <01MH8P7FKTK60008EQ@Macon2.Mercer.edu> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273C94@LSRIEXCH1.lsmaster.lifespan.org> The problems in sectioning mealworms are the same as in sectioning fruitflies and other arthropods, primarily the resistance of their chitinous integument to processing solvents and to sectioning. There are a few good methods for softening chitin which have been mentioned on Histonet previously. Perhaps you can find them in the archives by searching for "chitin". I haven't sectioned mealworms but I have successfully used one such method in sectioning fruitfly heads, which I will be glad to share if you can't locate the information. From rchiovetti <@t> yahoo.com Thu May 31 18:20:18 2007 From: rchiovetti <@t> yahoo.com (Robert Chiovetti) Date: Thu May 31 18:20:22 2007 Subject: [Histonet] processing fatty tissue Message-ID: <387005.30260.qm@web58913.mail.re1.yahoo.com> Maxim, Rene (and Other Histonetters), That's interesting re: using either a mix of ethanol+isopropanol+mineral oil (Rene) or isopropanol+mineral oil (Maxim) for breast tissue. Could you share your recipes with us? I have a customer (derm path) who could probably benefit from this for larger and thicker skin specimens which sometimes have a lot of subcutaneous fat associated with them. Thanks in advance, if you could share the recipes! Cheers, Bob Robert (Bob) Chiovetti, Ph.D. Southwest Precision Instruments The Desert Southwest's Microscopy Resource Tucson, Arizona USA Tel./Fax 520-546-4986 www.swpinet.com Member, Arizona Small Business Association (www.asba.com) ----- Original Message ---- From: Maxim Peshkov To: ROrr@enh.org Cc: histonet@lists.utsouthwestern.edu; Gervaip@aol.com Sent: Thursday, May 31, 2007 12:05:21 PM Subject: Re: [Histonet] processing fatty tissue I completely agree with Rene. When we processed breasts (and other tissues) with isopropanol+mineral oil that we ceased to feel the difficulties with our samples. We have manually processing. More thanks to Dr. McCormick for cassettes! Sincerely, Maxim Peshkov Russia Taganrog "Rene J Buesa" wrote: Date: Tue, 29 May 2007 06:22:59 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] processing fatty tissue To: "Orr, Rebecca" , histonet@lists.utsouthwestern.edu Cc: Gervaip@aol.com 1- thin slices 2- well fixed after the slices are prepared (at least 8 h) 3- use fresh alcohols for dehydration 4- extend somewaht (20%) the clearing steps time (time x 1.2), and 5- if possible use a 60?C paraffin, OR process with ethanol followed by a mixture of ethanol+propanol+mineral oil, and breats will cut "like butter". Ren? J. ____________________________________________________________________________________ Be a PS3 game guru. Get your game face on with the latest PS3 news and previews at Yahoo! Games. http://videogames.yahoo.com/platform?platform=120121 From kwuny <@t> EMAIL.CS.NSW.GOV.AU Thu May 31 20:57:47 2007 From: kwuny <@t> EMAIL.CS.NSW.GOV.AU (Young Kwun) Date: Thu May 31 20:54:56 2007 Subject: [Histonet] Help with MDM2 Protein Immunostaining In-Reply-To: <6.0.0.22.1.20070531083713.01b3abf8@gemini.msu.montana.edu> Message-ID: <200706011154622.SM01256@crgcsls814> Dear Gayle, Thank you for your reply about the MDM2 staining. I used citrate buffer in the Decloaker (a pressure cooker) for 5 min and incubated with primary antibody overnight at room temperature and used a polymer detection system (Ultravision) from LabVision for 30 min. I also tried EDTA buffer and protease without much success. With BondMax autostainer, I used pretreatment for 20 or 30 mininutes with two different buffers (ER1-citrate based and ER2-high pH buffer) and ER2 gave much sharper nuclear staining. The primary incubation time was 30 minutes. The detection kit I used was Bond Polymer Refine Detection kit. Thank you. Young Kwun Senior Hospital Scientist Dept of Anatomical Pathology Concord Hospital Concord NSW 2139 Australia Phone:61-2-9767 6075 Fax:61-2-9767 8427 Email:kwuny@email.cs.nsw.gov.au -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: Friday, 1 June 2007 12:38 AM To: Young Kwun Subject: Re: [Histonet] Help with MDM2 Protein Immunostaining If you tell us HOW you do your protocol, then people can help with your background staining - which can come from many sources? At 05:53 PM 5/30/2007, you wrote: >Dear Colleagues, > > > >Does anyone in Histoland use MDM2 Protein staining for some soft tissue >sarcomas successfully? > >I tried Lab Vision's polyclonal antibody (1/100 dil) and Novocastra's MDM2 >(clone 1B10, 1/150 dil) with Decloaker HIER and BondMax Autostainer. > >However, my pathologist is not happy with the staining results, mainly >because of some non-specific stains. > >Any help would be appreciated. Thank you. > > > > > > > >Young Kwun > >Senior Hospital Scientist > >Dept of Anatomical Pathology > >Concord Hospital > >Concord NSW 2139 > >Australia > >Phone:61-2-9767 6075 > >Fax:61-2-9767 8427 > >Email:kwuny@email.cs.nsw.gov.au > > > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 _____________________________________________________________________ This email has been scanned for the Sydney South West Area Health Service by the MessageLabs Email Security System. SSWAHS regularly monitors emails and attachments to ensure compliance with the NSW Government's Electronic Messaging Policy. "This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please destroy it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of the Sydney South West Area Health Service." From zumbor <@t> email.cs.nsw.gov.au Thu May 31 23:12:33 2007 From: zumbor <@t> email.cs.nsw.gov.au (Rosalba) Date: Thu May 31 23:18:35 2007 Subject: [Histonet] Plastic embedding Message-ID: <01C7A456.E6CC7450.zumbor@email.cs.nsw.gov.au> Hi All, Does anyone know of a reference source either a book or website which explains plastic embedding from start to finish and all the parphenalia required. Thanks Rosalba Zumbo Supervisor Histology Dept Department of Forensic Medicine 42-50 Parramatta Rd Glebe NSW 2037 Australia PH: 61 2 85847842 FAX: 61 2 95664573 zumbor@email.cs.nsw.gov.au "This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please destroy it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of the Sydney South West Area Health Service." From springrosycloud <@t> 126.com Thu May 24 22:26:47 2007 From: springrosycloud <@t> 126.com (=?gb2312?B?1qO0us+8?=) Date: Fri Jul 13 08:33:59 2007 Subject: [Histonet] (no subject) Message-ID: <4656575A.00018F.01757@bj126app3.126.com> Hello, everyone! I stained frozen and paraffin-embedded sections of hum= an skeleton and kidney tissues using the Anti-=A6=C1-Dystroglycan antibod= y, clone VIA4-1(catalog #: 05-298) from Upstate biotechnology company by = immunohistochemistry. The method is: 3um sections, EDTA 8.0 heat retrieva= l(pressure cooking) 10min for paraffin section=A3=ACantibody dilution is = 1:50,1:100 and 1:200(25 degree, 2 hours), and addition of EnVision antibo= dy complex, and then DAB. But there was no any positive signals.=20 I do not know what is the matter. Please help me. Now, I need to know = whether the dilution or reaction time is suitable. Please give me some go= od suggestions. I am eager to using the antibody smoothly. Thank you very= much. Sincerely, =20 Chunxia Zheng PhD Research Institute of Nephrology, Jinling Hospital 305 East Zhongshan Road Nanjing 210002, China Phone: 86-25-80860218 Fax: 86-25-84801992 =20 =20 =20 =C8=CB=C9=BD=C8=CB=BA=A3=CA=A2=BE=B0=A3=AC=BE=A1=D4=DA=C3=CE=BB=C3=CE=F7=D3= =CE =20