[Histonet] Gill's hematoxylin

Rene J Buesa rjbuesa <@t> yahoo.com
Mon Mar 19 13:14:48 CDT 2007


If you use what you have you will end with another hematoxylin. Try it, maybe it is good afterall!
René J.

"Wurdak, Elizabeth" <EWURDAK <@t> CSBSJU.EDU> wrote:  Hello Histonetters,
We have decided to switch from Harris' hematoxylin to Gill's to avoid using
mercury. I have a couple of recipes for Gill's hematoxylin. They both call
for aluminum sulfate and sodium iodate. I have only ammonia alum,
NH4Al(SO4)2x12H2O, or potassium alum, KAl(SO4)2x12H2O and sodium periodate
on hand. Can I use these reagents instead and do I need to modify the
quantities of each. The IHC World recipe requires 0.2g sodium iodate and
20g aluminum sulfate for 1020 ml of the single strength hematoxylin (2g)
solution.
Thanks for any help you can give me.
Liz

Elizabeth Wurdak, PhD
Biology Department
Saint John¹s University
Collegeville, MN 56321
Tel: (320) 363-3177



On 3/19/07 8:58 AM, "SHargrove <@t> urhcs.org" wrote:

> Regarding the processing of prostate biopsies, We have a "short run" on one
> of the VIP's that we use for all biopsies. Our alcohols are graded from 70%
> - 100%. We used to use sponges for ours and the pathologist thought it
> caused some breaking. Imagine the tissue bouncing off of the pores of the
> sponge. Now all needle biopsies are wrapped in paper before processing. We
> are having much better results .We still use sponges for biopsies. Be sure
> the sponges are saturated with formalin before use, and they will not
> float.
> Susie Hargrove
> Tech Specialist Histology
> United Regional
> Wichita Falls, Texas
> 
> **********************************************************************
> The documents inside this electronic transmission contain
> confidential information belonging to the sender that is legally
> privileged. This information is intended only for the use of the
> individual or entity named above. The authorized recipient of this
> information is prohibited from disclosing this information to any
> other party and is required to destroy the information after its
> stated need has been fulfilled, unless otherwise required by law.
> 
> If you are not the intended recipient, you are hereby notified that
> any disclosure, copying, distribution, or action taken in reliance
> on the contents of these documents is strictly prohibited. If you
> received this electronic transmission in error, please notify the
> sender immediately to arrange for return.
> **********************************************************************
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




 
---------------------------------
Never miss an email again!
Yahoo! Toolbar alerts you the instant new Mail arrives. Check it out.


More information about the Histonet mailing list