[Histonet] Choosing a blocking reagent

[Jennifer Harvey]

We have been hotly debating this subject for over a week in our lab.

How do you choose the blocking reagent for immunohistochemistry?

If you are using a secondary made in a donkey can you just use any hoofed
animal's serum to block??


Thanks


Jennifer Harvey


Center for Stem Cell Biology
Department of Cell and Dev Biology
Vanderbilt University
831 Light Hall
2215 Garland Ave.
Nashville, TN  37232-0225
Phone: 615-322-4378
Fax: 615-343-2173
jennifer.harvey <@t> vanderbilt.edu