From BMolinari <@t> heart.thi.tmc.edu Thu Mar 1 05:33:16 2007 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Thu Mar 1 05:33:21 2007 Subject: [Histonet] Purple Haze In-Reply-To: Message-ID: EXCELLENT!!! Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, R.E. Sent: Wednesday, February 28, 2007 9:37 AM To: Jason Wickersham; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Purple Haze HEY JOE WHERE YOU GOING WITH THAT TOE IN YOUR HAND??. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jason Wickersham Sent: 28 February 2007 15:26 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Purple Haze ...Excuse me while I kiss this "slide"... Jason Wickersham Application Specialist Product Unit Electron Microscopy OLYMPUS SOFT IMAGING SOLUTIONS 84 E Grand Avenue Montvale, NJ 07645 Tel: 551-804-1845 Email: Jason.Wickersham@Olympus-SIS.com http://www.soft-imaging.net and http://www.olympus-sis.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, February 27, 2007 1:12 PM To: 'Fred Underwood'; lblazek@digestivespecialists.com; RJLevier@LancasterGeneral.org; ree3@leicester.ac.uk; histonet@lists.utsouthwestern.edu; jnocito@satx.rr.com; JWEEMS@sjha.org Subject: RE: [Histonet] Purple Haze Now you are talking! Hair Metal was/is great! Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fred Underwood Sent: Tuesday, February 27, 2007 8:12 AM To: lblazek@digestivespecialists.com; RJLevier@LancasterGeneral.org; ree3@leicester.ac.uk; histonet@lists.utsouthwestern.edu; jnocito@satx.rr.com; JWEEMS@sjha.org Subject: RE: [Histonet] Purple Haze How about a shout out to the "hair metal" days of the '80's. Does everyone remember Winger's version of Purple Haze. >>> "Blazek, Linda" 2/27/2007 7:18 AM >>> Prince was Purple Rain! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, February 26, 2007 9:26 PM To: Joe Nocito; Edwards, R.E.; LeVier, Rebecca J; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Purple Haze Poor Joe the Toe... Not Jimmy - Prince. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Monday, February 26, 2007 6:58 PM To: Edwards, R.E.; LeVier, Rebecca J; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Purple Haze is that a Jimmy Hendrix song? Seriously Becky, are you staining your H&Es with an automatic stainer? We were getting the same problem until we checked the flow of our rinse water. Someone with a sense of humor turned the wrong valve and instead of shutting the water off, they decreased the flow. JTT ----- Original Message ----- From: "Edwards, R.E." To: "LeVier, Rebecca J" ; Sent: Monday, February 26, 2007 9:20 AM Subject: RE: [Histonet] Purple Haze Well that would be a new experience to me. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LeVier, Rebecca J Sent: 26 February 2007 14:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Purple Haze Hi Folks, We have encountered an artifact which we call "purple haze" where the tissue looks as it waited too long to be fixed. It is not every piece of tissue in a cassette. I know that others have had this problem in the past and I was wondering what you have done to fix this. We also have had this problem and to fix it, we changed processors. We are now experiencing this problem again. If any one can help, I would really appreciate it. Thanks in advance! Becky Email: rjlevier@lancastergeneral.org Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. 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Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Barry.R.Rittman <@t> uth.tmc.edu Thu Mar 1 06:14:13 2007 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Thu Mar 1 06:14:19 2007 Subject: [Histonet] External diameter of large vein References: <00a201c75bb8$971f00d0$711480cb@piii> Message-ID: As the tunica adventitia is fairly thick and merges into the surounding connective tissue it is difficult to measure. I suspect this vein does not have any muscle in the adventitia as many of the largest veins do. I would suggest one of the below: Take a photograph and also of a micrometer at same magnification. If too large for your objectives either use a dissecting microscope or place the slide on a light box and use a camera. This will probably give you the most accurate measure. Measure from lumen up to the border between the tunica adventitia and tunica media. This is probably the most useful. There are several automatic stages that have been used in image analysis. Not sure why you don't want to use the vernier scale on the microsope stage as this is usually accurate. Barry ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Tahseen Sent: Wed 2/28/2007 10:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] External diameter of large vein Dear All, How can the external diameter of large vein like renal vein be measured on slide under light microscope? As the vein is large and measures several millimeters the micrometer scale cannot be used. we also do not want to measure grossly using a venire calipre. Our aim is to measure the vertical, oblique and transverse external diameter on the slide through microscope. Please also give the reference of the method. Thanks Dr Robina Shaheen Abbottabad, Pakistan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kildee6093 <@t> mac.com Thu Mar 1 08:46:45 2007 From: kildee6093 <@t> mac.com (Marilyn McDonald) Date: Thu Mar 1 08:47:00 2007 Subject: [Histonet] Thank you Message-ID: Just a note to thank everyone who responded with their favorite LIS. My friend was impressed to say the least now the task is to decide. The Hisotnet is a great place to get answers one needs. Thank you again. Marilyn From jhnspam <@t> aol.com Thu Mar 1 10:00:03 2007 From: jhnspam <@t> aol.com (jhnspam@aol.com) Date: Thu Mar 1 10:00:18 2007 Subject: [Histonet] X-gal staining Message-ID: <8C92A31CED40A29-18CC-452E@FWM-D11.sysops.aol.com> Does anyone have a good procedure for X-Gal staining of frozen tissue sections? I keep getting only non specific staining with the procedure I have. I would also like a procedure for whole mount staining as well. Thanks very much for your help!!! Pam Johnson Supervisor, ARC Histology 332 N. Lauderdale St. Memphis, TN 38105-2794 901-495-2272, office 901-495-3112, fax ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From sbanwait <@t> buckinstitute.org Thu Mar 1 10:46:57 2007 From: sbanwait <@t> buckinstitute.org (Surita Banwait) Date: Thu Mar 1 10:47:05 2007 Subject: [Histonet] X-gal staining In-Reply-To: <8C92A31CED40A29-18CC-452E@FWM-D11.sysops.aol.com> Message-ID: I would be very interested in some suggestions as well... we have tried whole mount staining on mouse brain without much success. We also tried paraffin processing samples and then staining with beta gal antibody from chemicon (also without much success). Thanks! Surita ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Surita Banwait Morphology & Imaging Core Research Associate II Buck Institute for Age Research 8001 Redwood Blvd. Novato, CA 94945 415-209-2221 sbanwait@buckinstitute.org ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jhnspam@aol.com Sent: Thursday, March 01, 2007 8:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] X-gal staining Does anyone have a good procedure for X-Gal staining of frozen tissue sections? I keep getting only non specific staining with the procedure I have. I would also like a procedure for whole mount staining as well. Thanks very much for your help!!! Pam Johnson Supervisor, ARC Histology 332 N. Lauderdale St. Memphis, TN 38105-2794 901-495-2272, office 901-495-3112, fax ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHosp.org Thu Mar 1 10:56:01 2007 From: Janet.Bonner <@t> FLHosp.org (Bonner, Janet) Date: Thu Mar 1 10:57:35 2007 Subject: [Histonet] question for all the mouse brain people out there! References: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D35BE@sjhaexc02.sjha.org> Message-ID: <5F31F38C96781A4FBE3196EBC22D478004A77C@fhosxchmb006.ADVENTISTCORP.NET> So it's not :>) ; Just :> . And it's not even Friday!!!! @:) ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Weems, Joyce Sent: Wed 2/28/2007 3:49 PM To: Patsy Ruegg; Kimberly Johnson; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] question for all the mouse brain people out there! No, just a bird brain...:>) getting more like a turkey every day. -----Original Message----- From: Patsy Ruegg [mailto:pruegg@ihctech.net] Sent: Wednesday, February 28, 2007 3:45 PM To: Weems, Joyce; 'Kimberly Johnson'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] question for all the mouse brain people out there! That made me laugh Joyce! Patsy Are you a mouse brained person??? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, February 28, 2007 10:01 AM To: Kimberly Johnson; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] question for all the mouse brain people out there! You must quit calling names if you want them to help!!! j:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Kimberly Johnson Sent: Wednesday, February 28, 2007 11:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] question for all the mouse brain people out there! Hello Histonet! I have a quick question for you mouse brain people out there who have been so very helpful to me in the past. I usually keep my brains in 30% sucrose for 2 days, then freeze them for sectioning. Would I ruin them if I kept them in the sucrose for 4 days? I am trying to get out of coming in on the weekend, so all you input would be greatly appreciated! Thanks so much! Kim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From JWEEMS <@t> sjha.org Thu Mar 1 11:00:04 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Thu Mar 1 11:00:56 2007 Subject: [Histonet] question for all the mouse brain people out there! In-Reply-To: <5F31F38C96781A4FBE3196EBC22D478004A77C@fhosxchmb006.ADVENTISTCORP.NET> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D35CC@sjhaexc02.sjha.org> 2 funny! Bring on Friday!!! -----Original Message----- From: Bonner, Janet [mailto:Janet.Bonner@FLHosp.org] Sent: Thursday, March 01, 2007 11:56 AM To: Weems, Joyce; Patsy Ruegg; Kimberly Johnson; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] question for all the mouse brain people out there! So it's not :>) ; Just :> . And it's not even Friday!!!! @:) _____ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Weems, Joyce Sent: Wed 2/28/2007 3:49 PM To: Patsy Ruegg; Kimberly Johnson; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] question for all the mouse brain people out there! No, just a bird brain...:>) getting more like a turkey every day. -----Original Message----- From: Patsy Ruegg [ mailto:pruegg@ihctech.net] Sent: Wednesday, February 28, 2007 3:45 PM To: Weems, Joyce; 'Kimberly Johnson'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] question for all the mouse brain people out there! That made me laugh Joyce! Patsy Are you a mouse brained person??? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, February 28, 2007 10:01 AM To: Kimberly Johnson; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] question for all the mouse brain people out there! You must quit calling names if you want them to help!!! j:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Kimberly Johnson Sent: Wednesday, February 28, 2007 11:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] question for all the mouse brain people out there! Hello Histonet! I have a quick question for you mouse brain people out there who have been so very helpful to me in the past. I usually keep my brains in 30% sucrose for 2 days, then freeze them for sectioning. Would I ruin them if I kept them in the sucrose for 4 days? I am trying to get out of coming in on the weekend, so all you input would be greatly appreciated! Thanks so much! Kim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From rjbuesa <@t> yahoo.com Thu Mar 1 10:57:48 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Mar 1 11:57:56 2007 Subject: [Histonet] External diameter of large vein In-Reply-To: <00a201c75bb8$971f00d0$711480cb@piii> Message-ID: <487348.45482.qm@web61213.mail.yahoo.com> Tahseen: As you are surely aware the whole process (fixation, processing, embedding and sectioning) will have an effect on the real diameter as compared with the fresh specimen. The fact that you will end with a diameter not exactly as the initial will give you some "latitude" in the measurement. In some occasions when I had needed to measure whole sections y just used a copying machine and copy the section. Then I was able to measure the copied image. This is a "low tech" approach but that have served me well in the past. I have been able to use a good ruler for the measurements. Sorry, no references though. This is mine! Ren? J. Tahseen wrote: Dear All, How can the external diameter of large vein like renal vein be measured on slide under light microscope? As the vein is large and measures several millimeters the micrometer scale cannot be used. we also do not want to measure grossly using a venire calipre. Our aim is to measure the vertical, oblique and transverse external diameter on the slide through microscope. Please also give the reference of the method. Thanks Dr Robina Shaheen Abbottabad, Pakistan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Cheap Talk? Check out Yahoo! Messenger's low PC-to-Phone call rates. From jerryd <@t> dcla.com Thu Mar 1 11:57:25 2007 From: jerryd <@t> dcla.com (Jerry Duncan) Date: Thu Mar 1 11:58:20 2007 Subject: [Histonet] slide drier Message-ID: <0420BA36C221714593AA90E7F92CAF4706B8DA73@sturgeon.dcla.com> Does anyone have a new or gently used slide drier they would like to sell? Jerry Duncan, HT (ASCP) DCL Medical Laboratories From Maxim_71 <@t> mail.ru Thu Mar 1 11:58:33 2007 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Thu Mar 1 11:59:54 2007 Subject: [Histonet] GRAM Stain Timing Question Message-ID: <53981664.20070301205833@mail.ru> Sara, Our protocol for GRAM: 1. Dewax. 2. DW, rinse (several secs). 3. 1% Aqv. Gentianviolet(+Tween20 5 drops in 100mL), 1 min. 4. DW, rinse (several secs). 5. Grams's iodine 1 min. 6. DW, rinse (several secs). 7. Blot section dry with filter paper. 8. ACETONE, 2-4 DIPS, until excess blue dye comes off of the sections. 9. Tap water several secs. 10. DW with 2-3 drops glacial acetic acid on 100mL, 30-60 secs. 11. 1% Aqv. Neutral red(+Tween20 5 drops; +2 drops glacial acetic acid in 100mL), 1 min. 12. Blot section dry with filter paper. 13. Acetone, 3 changes, each 10 dips. 14. Xylene, 2 changes, each 10 dips. 15. DPX, coverslip. All reagents we prepare by itself. This protocol we doing manually, daily, many years. It is work great. Pathologists are lucky, and techs also. Good luck, Maxim Peshkov Taganrog Russia, Maxim_71@mail.ru From sbreeden <@t> nmda.nmsu.edu Thu Mar 1 12:32:47 2007 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Mar 1 12:32:59 2007 Subject: [Histonet] GRAM Thanks You! Message-ID: <4D14F0FC9316DD41972D5F03C070908B8F4367@nmdamailsvr.nmda.ad.nmsu.edu> Thank you to everyone that responded to my request for help tightening up my differentiation times for the GRAM stain. I'm sure that the comments will help me give a better slide to my pathologists! And how amazing to me that one respondent is from Russia! We have such a great wealth of resources from so many places - we are so fortunate! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 From RSRICHMOND <@t> aol.com Thu Mar 1 12:44:20 2007 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Thu Mar 1 12:44:34 2007 Subject: [Histonet] Re: GRAM Stain Timing Question Message-ID: The Gram stain is named for Hans Christian Gram (Danish, like the storyteller), 1853-1938, published 1884. There are several adaptations for tissue sections, perhaps Brown-Brenn and Brown-Hopps being the best known. The Gram stain is useful for smears, much less so for tissue sections, and its use by pathologists should be discouraged. If you want to look for or count bacteria in tissue sections, a simple Giemsa (or Diff-Quik II, or toluidine blue) stain is much to be preferred. Acetone decolorization is much more rapid that alcohol decolorization. Bob Richmond Samurai Pathologist Knoxville TN ************************************** AOL now offers free email to everyone. Find out more about what's free from AOL at http://www.aol.com. From srishan <@t> mail.holyname.org Thu Mar 1 14:40:30 2007 From: srishan <@t> mail.holyname.org (srishan@mail.holyname.org) Date: Thu Mar 1 14:41:00 2007 Subject: [Histonet] regarding breast tissue fixation Message-ID: Hi All, Does anyone have a solution for the new ASCO/CAP guidelines regarding the fixation time in formalin for the breast tissue/HER2. Apart from the labs who work 7day/24 hours, what is everyone else doing about the weekends and long weekends? Any information or guidance will be greatly appreciated. Thank You in advance Nirmala Srishan Histology Supervisor Holy Name Hospital Teaneck NJ 07666 From rjbuesa <@t> yahoo.com Thu Mar 1 14:56:22 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Mar 1 15:03:09 2007 Subject: [Histonet] regarding breast tissue fixation In-Reply-To: Message-ID: <602584.10600.qm@web61218.mail.yahoo.com> I advise you to go to "Histonet Archieves"; thare was an ample discussion about the subject about 2 weeks ago. Ren? J. srishan@mail.holyname.org wrote: Hi All, Does anyone have a solution for the new ASCO/CAP guidelines regarding the fixation time in formalin for the breast tissue/HER2. Apart from the labs who work 7day/24 hours, what is everyone else doing about the weekends and long weekends? Any information or guidance will be greatly appreciated. Thank You in advance Nirmala Srishan Histology Supervisor Holy Name Hospital Teaneck NJ 07666 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your own web address. Have a HUGE year through Yahoo! Small Business. From AnthonyH <@t> chw.edu.au Thu Mar 1 16:02:11 2007 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Mar 1 16:02:33 2007 Subject: [Histonet] External diameter of large vein Message-ID: What I would do is scan the slide using a routine document scanner fitted with a back light (used to scan 35mm slides). Include a ruler in the scan. Print out the image and measure. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 > Bandaged Bear Day - Friday 30th March 2007 > For more information and to order merchandise visit www.bandagedbearday.com.au > > -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tahseen Sent: Thursday, 1 March 2007 3:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] External diameter of large vein Dear All, How can the external diameter of large vein like renal vein be measured on slide under light microscope? As the vein is large and measures several millimeters the micrometer scale cannot be used. we also do not want to measure grossly using a venire calipre. Our aim is to measure the vertical, oblique and transverse external diameter on the slide through microscope. Please also give the reference of the method. Thanks Dr Robina Shaheen Abbottabad, Pakistan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From POWELL_SA <@t> Mercer.edu Thu Mar 1 16:28:23 2007 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Thu Mar 1 16:29:10 2007 Subject: [Histonet] External diameter of large vein In-Reply-To: Message-ID: <01MDPNUF0ATI8WYDQN@Macon2.Mercer.edu> Martin Microscopes has this suggestion for those with Photoshop. Go to http://www.martinmicroscope.com/CalibrationChart.htm for instructions. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Thursday, March 01, 2007 5:02 PM To: Tahseen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] External diameter of large vein What I would do is scan the slide using a routine document scanner fitted with a back light (used to scan 35mm slides). Include a ruler in the scan. Print out the image and measure. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 > Bandaged Bear Day - Friday 30th March 2007 > For more information and to order merchandise visit www.bandagedbearday.com.au > > -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tahseen Sent: Thursday, 1 March 2007 3:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] External diameter of large vein Dear All, How can the external diameter of large vein like renal vein be measured on slide under light microscope? As the vein is large and measures several millimeters the micrometer scale cannot be used. we also do not want to measure grossly using a venire calipre. Our aim is to measure the vertical, oblique and transverse external diameter on the slide through microscope. Please also give the reference of the method. Thanks Dr Robina Shaheen Abbottabad, Pakistan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Thu Mar 1 18:43:07 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Mar 1 18:43:13 2007 Subject: [Histonet] GRAM Stain Timing Question References: <4D14F0FC9316DD41972D5F03C070908B8F435F@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <000701c75c63$bf059400$649eae18@yourxhtr8hvc4p> Sara, we have a wash bottle with acetone and rinse the slides until the Crystal Violet stops dripping off the slides, just like Gram smears in Micro. I'm in a haze again. This time it's Scotch. Is it Friday yet? JTT ----- Original Message ----- From: "Breeden, Sara" To: Sent: Wednesday, February 28, 2007 1:15 PM Subject: [Histonet] GRAM Stain Timing Question I seem to do a lot of GRAM stains and although I've modified some of the times to suit my pathologists' tastes, I have never been able to pin down the first differentiation time in 100% alcohol. Today, I'm using 10 quick dips although it's only because the wind is blowing and the moon is on the rise. Can someone give me a more specific guideline for differentiating after the Gram's Iodine and before the Safranin? I'd like to leave a more definite legacy when I retire than "differentiate in 100% alcohol or acetone"... I'm thankin' you in advance. And I think we all know who the Purple Haze Generation are on THIS media, don't we??? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Thu Mar 1 18:44:48 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Mar 1 18:44:54 2007 Subject: [Histonet] GRAM Stain Timing Question References: <4D14F0FC9316DD41972D5F03C070908B8F435F@nmdamailsvr.nmda.ad.nmsu.edu> <6CBA6DC98A079D408C87250591D9DFB802360DFC@bruexchange.digestivespecialists.com> Message-ID: <003001c75c63$fb7bc9e0$649eae18@yourxhtr8hvc4p> I love a woman who talks dirty. Scotch? Did some one say Scotch? JTT ----- Original Message ----- From: "Blazek, Linda" To: "Breeden, Sara" ; Sent: Wednesday, February 28, 2007 1:47 PM Subject: RE: [Histonet] GRAM Stain Timing Question Can't your legacy be "differentiate until it looks right"? If I recall right (and that is a question in it's self) when the color stops running off the slide the differentiation is complete. To go any further you will decolorize the gram-positive thingy bobs. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, February 28, 2007 2:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GRAM Stain Timing Question I seem to do a lot of GRAM stains and although I've modified some of the times to suit my pathologists' tastes, I have never been able to pin down the first differentiation time in 100% alcohol. Today, I'm using 10 quick dips although it's only because the wind is blowing and the moon is on the rise. Can someone give me a more specific guideline for differentiating after the Gram's Iodine and before the Safranin? I'd like to leave a more definite legacy when I retire than "differentiate in 100% alcohol or acetone"... I'm thankin' you in advance. And I think we all know who the Purple Haze Generation are on THIS media, don't we??? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Thu Mar 1 18:46:05 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Mar 1 18:46:32 2007 Subject: [Histonet] Purple Haze References: <200702271828.l1RIS616009669@mail.soft-imaging.de> <78B53BA1C5A2D9449EDA30A98800EC947FC14B@ms-s-gws.soft-imaging.net> Message-ID: <005201c75c64$2c0d42a0$649eae18@yourxhtr8hvc4p> Lunch, of course. ----- Original Message ----- From: "Edwards, R.E." To: "Jason Wickersham" ; Sent: Wednesday, February 28, 2007 9:36 AM Subject: RE: [Histonet] Purple Haze HEY JOE WHERE YOU GOING WITH THAT TOE IN YOUR HAND??. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jason Wickersham Sent: 28 February 2007 15:26 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Purple Haze ...Excuse me while I kiss this "slide"... Jason Wickersham Application Specialist Product Unit Electron Microscopy OLYMPUS SOFT IMAGING SOLUTIONS 84 E Grand Avenue Montvale, NJ 07645 Tel: 551-804-1845 Email: Jason.Wickersham@Olympus-SIS.com http://www.soft-imaging.net and http://www.olympus-sis.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, February 27, 2007 1:12 PM To: 'Fred Underwood'; lblazek@digestivespecialists.com; RJLevier@LancasterGeneral.org; ree3@leicester.ac.uk; histonet@lists.utsouthwestern.edu; jnocito@satx.rr.com; JWEEMS@sjha.org Subject: RE: [Histonet] Purple Haze Now you are talking! Hair Metal was/is great! Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fred Underwood Sent: Tuesday, February 27, 2007 8:12 AM To: lblazek@digestivespecialists.com; RJLevier@LancasterGeneral.org; ree3@leicester.ac.uk; histonet@lists.utsouthwestern.edu; jnocito@satx.rr.com; JWEEMS@sjha.org Subject: RE: [Histonet] Purple Haze How about a shout out to the "hair metal" days of the '80's. Does everyone remember Winger's version of Purple Haze. >>> "Blazek, Linda" 2/27/2007 7:18 AM >>> Prince was Purple Rain! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, February 26, 2007 9:26 PM To: Joe Nocito; Edwards, R.E.; LeVier, Rebecca J; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Purple Haze Poor Joe the Toe... Not Jimmy - Prince. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Monday, February 26, 2007 6:58 PM To: Edwards, R.E.; LeVier, Rebecca J; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Purple Haze is that a Jimmy Hendrix song? Seriously Becky, are you staining your H&Es with an automatic stainer? We were getting the same problem until we checked the flow of our rinse water. Someone with a sense of humor turned the wrong valve and instead of shutting the water off, they decreased the flow. JTT ----- Original Message ----- From: "Edwards, R.E." To: "LeVier, Rebecca J" ; Sent: Monday, February 26, 2007 9:20 AM Subject: RE: [Histonet] Purple Haze Well that would be a new experience to me. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LeVier, Rebecca J Sent: 26 February 2007 14:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Purple Haze Hi Folks, We have encountered an artifact which we call "purple haze" where the tissue looks as it waited too long to be fixed. It is not every piece of tissue in a cassette. I know that others have had this problem in the past and I was wondering what you have done to fix this. We also have had this problem and to fix it, we changed processors. We are now experiencing this problem again. If any one can help, I would really appreciate it. Thanks in advance! Becky Email: rjlevier@lancastergeneral.org Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Fri Mar 2 03:56:56 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Fri Mar 2 03:57:14 2007 Subject: [Histonet] Purple Haze In-Reply-To: <005201c75c64$2c0d42a0$649eae18@yourxhtr8hvc4p> References: <005201c75c64$2c0d42a0$649eae18@yourxhtr8hvc4p> Message-ID: So what's on todays menu?, "TOED IN THE HOLE" -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: 02 March 2007 00:46 To: Edwards, R.E.; Jason Wickersham; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Purple Haze Lunch, of course. ----- Original Message ----- From: "Edwards, R.E." To: "Jason Wickersham" ; Sent: Wednesday, February 28, 2007 9:36 AM Subject: RE: [Histonet] Purple Haze HEY JOE WHERE YOU GOING WITH THAT TOE IN YOUR HAND??. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jason Wickersham Sent: 28 February 2007 15:26 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Purple Haze ...Excuse me while I kiss this "slide"... Jason Wickersham Application Specialist Product Unit Electron Microscopy OLYMPUS SOFT IMAGING SOLUTIONS 84 E Grand Avenue Montvale, NJ 07645 Tel: 551-804-1845 Email: Jason.Wickersham@Olympus-SIS.com http://www.soft-imaging.net and http://www.olympus-sis.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, February 27, 2007 1:12 PM To: 'Fred Underwood'; lblazek@digestivespecialists.com; RJLevier@LancasterGeneral.org; ree3@leicester.ac.uk; histonet@lists.utsouthwestern.edu; jnocito@satx.rr.com; JWEEMS@sjha.org Subject: RE: [Histonet] Purple Haze Now you are talking! Hair Metal was/is great! Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fred Underwood Sent: Tuesday, February 27, 2007 8:12 AM To: lblazek@digestivespecialists.com; RJLevier@LancasterGeneral.org; ree3@leicester.ac.uk; histonet@lists.utsouthwestern.edu; jnocito@satx.rr.com; JWEEMS@sjha.org Subject: RE: [Histonet] Purple Haze How about a shout out to the "hair metal" days of the '80's. Does everyone remember Winger's version of Purple Haze. >>> "Blazek, Linda" 2/27/2007 7:18 AM >>> Prince was Purple Rain! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, February 26, 2007 9:26 PM To: Joe Nocito; Edwards, R.E.; LeVier, Rebecca J; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Purple Haze Poor Joe the Toe... Not Jimmy - Prince. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Monday, February 26, 2007 6:58 PM To: Edwards, R.E.; LeVier, Rebecca J; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Purple Haze is that a Jimmy Hendrix song? Seriously Becky, are you staining your H&Es with an automatic stainer? We were getting the same problem until we checked the flow of our rinse water. Someone with a sense of humor turned the wrong valve and instead of shutting the water off, they decreased the flow. JTT ----- Original Message ----- From: "Edwards, R.E." To: "LeVier, Rebecca J" ; Sent: Monday, February 26, 2007 9:20 AM Subject: RE: [Histonet] Purple Haze Well that would be a new experience to me. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LeVier, Rebecca J Sent: 26 February 2007 14:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Purple Haze Hi Folks, We have encountered an artifact which we call "purple haze" where the tissue looks as it waited too long to be fixed. It is not every piece of tissue in a cassette. I know that others have had this problem in the past and I was wondering what you have done to fix this. We also have had this problem and to fix it, we changed processors. We are now experiencing this problem again. If any one can help, I would really appreciate it. Thanks in advance! Becky Email: rjlevier@lancastergeneral.org Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BMolinari <@t> heart.thi.tmc.edu Fri Mar 2 05:55:13 2007 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri Mar 2 05:55:17 2007 Subject: [Histonet] Purple Haze In-Reply-To: Message-ID: I'll never be able to listen to "Hey Joe" quite the same way again. My Dad was the same way; he thought he was so funny! We would just roll our eyes and groan! Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: Edwards, R.E. [mailto:ree3@leicester.ac.uk] Sent: Thursday, March 01, 2007 5:39 AM To: Molinari, Betsy Subject: RE: [Histonet] Purple Haze Thanks, it did make me smile as I typed it, as my daughters say I always laugh at my own jokes, which they regard as not terribly amusing. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: 01 March 2007 11:33 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Purple Haze EXCELLENT!!! Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, R.E. Sent: Wednesday, February 28, 2007 9:37 AM To: Jason Wickersham; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Purple Haze HEY JOE WHERE YOU GOING WITH THAT TOE IN YOUR HAND??. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jason Wickersham Sent: 28 February 2007 15:26 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Purple Haze ...Excuse me while I kiss this "slide"... Jason Wickersham Application Specialist Product Unit Electron Microscopy OLYMPUS SOFT IMAGING SOLUTIONS 84 E Grand Avenue Montvale, NJ 07645 Tel: 551-804-1845 Email: Jason.Wickersham@Olympus-SIS.com http://www.soft-imaging.net and http://www.olympus-sis.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, February 27, 2007 1:12 PM To: 'Fred Underwood'; lblazek@digestivespecialists.com; RJLevier@LancasterGeneral.org; ree3@leicester.ac.uk; histonet@lists.utsouthwestern.edu; jnocito@satx.rr.com; JWEEMS@sjha.org Subject: RE: [Histonet] Purple Haze Now you are talking! Hair Metal was/is great! Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fred Underwood Sent: Tuesday, February 27, 2007 8:12 AM To: lblazek@digestivespecialists.com; RJLevier@LancasterGeneral.org; ree3@leicester.ac.uk; histonet@lists.utsouthwestern.edu; jnocito@satx.rr.com; JWEEMS@sjha.org Subject: RE: [Histonet] Purple Haze How about a shout out to the "hair metal" days of the '80's. Does everyone remember Winger's version of Purple Haze. >>> "Blazek, Linda" 2/27/2007 7:18 AM >>> Prince was Purple Rain! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, February 26, 2007 9:26 PM To: Joe Nocito; Edwards, R.E.; LeVier, Rebecca J; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Purple Haze Poor Joe the Toe... Not Jimmy - Prince. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Monday, February 26, 2007 6:58 PM To: Edwards, R.E.; LeVier, Rebecca J; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Purple Haze is that a Jimmy Hendrix song? Seriously Becky, are you staining your H&Es with an automatic stainer? We were getting the same problem until we checked the flow of our rinse water. Someone with a sense of humor turned the wrong valve and instead of shutting the water off, they decreased the flow. JTT ----- Original Message ----- From: "Edwards, R.E." To: "LeVier, Rebecca J" ; Sent: Monday, February 26, 2007 9:20 AM Subject: RE: [Histonet] Purple Haze Well that would be a new experience to me. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LeVier, Rebecca J Sent: 26 February 2007 14:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Purple Haze Hi Folks, We have encountered an artifact which we call "purple haze" where the tissue looks as it waited too long to be fixed. It is not every piece of tissue in a cassette. I know that others have had this problem in the past and I was wondering what you have done to fix this. We also have had this problem and to fix it, we changed processors. We are now experiencing this problem again. If any one can help, I would really appreciate it. Thanks in advance! Becky Email: rjlevier@lancastergeneral.org Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jmahoney <@t> alegent.org Fri Mar 2 07:10:09 2007 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Fri Mar 2 07:45:00 2007 Subject: [Histonet] RE: Purple Haze Message-ID: <45E7CDD10200003C00006C44@gwia.alegent.org> For the young ones who could remember but don't because they are too young and for the old one who want to remember but can't because they are too old. Purple Haze lyrics by Jimi Hendrix Purple Haze was in my brain, lately things don't seem the same, actin' funny but I don't know why 'scuse me while I kiss the sky. Purple Haze all around, don't know if I'm coming up or down. Am I happy or in misery? Whatever it is, that girl put a spell on me. Purple Haze was in my eyes, don't know if it's day or night, you've got me blowing, blowing my mind is it tomorrow or just the end of time? From ree3 <@t> leicester.ac.uk Fri Mar 2 07:56:05 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Fri Mar 2 07:56:22 2007 Subject: [Histonet] RE: Purple Haze In-Reply-To: <45E7CDD10200003C00006C44@gwia.alegent.org> References: <45E7CDD10200003C00006C44@gwia.alegent.org> Message-ID: Well thanks for that, it was all a bit of a haze back then, and some of it was decidedly purple!. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: 02 March 2007 13:10 To: histonet@pathology.swmed.edu Subject: [Histonet] RE: Purple Haze For the young ones who could remember but don't because they are too young and for the old one who want to remember but can't because they are too old. Purple Haze lyrics by Jimi Hendrix Purple Haze was in my brain, lately things don't seem the same, actin' funny but I don't know why 'scuse me while I kiss the sky. Purple Haze all around, don't know if I'm coming up or down. Am I happy or in misery? Whatever it is, that girl put a spell on me. Purple Haze was in my eyes, don't know if it's day or night, you've got me blowing, blowing my mind is it tomorrow or just the end of time? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sonya.martin <@t> soton.ac.uk Fri Mar 2 08:00:44 2007 From: sonya.martin <@t> soton.ac.uk (Martin S.) Date: Fri Mar 2 08:01:19 2007 Subject: [Histonet] Background in frozen pancreas sections (continued...) References: <473337081a3c.45d99d6c@med.cornell.edu> <71437982F5B13A4D9A5B2669BDB89EE4023E350F@ISS-CL-EX-V1.soton.ac.uk> Message-ID: <71437982F5B13A4D9A5B2669BDB89EE4023E3521@ISS-CL-EX-V1.soton.ac.uk> Success! I followed your advice - Avidin/Biotin block, serum in the antibody diluents and preincubation of the goat anti-rat and I got some great staining with no background - Thanks! I don't know if it was all down the avidin/biotin rather than SA/biotin but I have had problems with the SA/B block before on other tissues giving more background than when I left it out. I use the ImmunoPure Peroxidase Suppressor from Pierce Biotech, its pricey but very convenient and you only need a little on each section. I've used it on mouse spleen, lymph node, pancreas and thyroid and human lymph node and tumours with excellent results. Thanks again for your help, Sonya -----Original Message----- From: Andrea Hooper [mailto:anh2006@med.cornell.edu] Sent: 20 February 2007 17:07 To: Martin S. Subject: RE: RE: [Histonet] Background in frozen pancreas sections (continued...) Interesting. My lab has had nothing but problems with the Streptavidin/Biotin blocking kit. Vector claims not to know what we are talking about but the background was SO MUCH WORSE using that kit, whereas when we changed to the Avidin/Biotin kit all background disappeared. Also about Vector secondaries ... I hate them! I never use anything but Jacksons. Without using the anti-rat IgG, mouse adsorbed from JAckson I think you will have difficulty getting rid of the background (though preaincubating with mouse serum or better yet pure mouse IgG is a good suggestion and may work). My recommendation is to invest the $100 and buy the mouse adsorbed donkey anti-rat IgG. Just looking over your protocol ... try blocking with 5% BSA in adddition to goat serum and use 1% BSA in all your antibody steps. I also hope you dilute your primary/secondary in some goat serum also. This will prevent your primary from binding to your blocking reagents (use 2% serum). Finally I have no experience with peroxidase suppressor ... is this stuff good? Good luck, let us know how it works out! Andrea >It was the Streptavidin/Biotin block I was using from Vector. Also, now >that I look at the antibodies again the goat anti-rat from Jackson >doesn't seem to be mouse adsorbed - however I did try a rabbit anti-rat >from Vector which was mouse adsorbed and the background was worse. >I also tried fixing with 95% ethanol straight after sections were cut >(instead of acetone) but saw no inprovement. >I've attached a few images to show you what I'm talking about - also in >the image x10-1 do you know what the mass of cells on the left is - >could it be a lymph node do you think? > >Next time I will try with the avidin/biotin block, longer washes and >try the mouse adsorbed anti-rat and also the goat anti-rat >pre-incubated in NMS for 1hr (as someone else suggested) - I'll let you >know how I get on! > >Heres my protocol; > >- Cut sections (10um) and air-dry 30mins >- Fix in 100% Acetone, 10min (room temp) >- Air-dry briefly and wash with PBS >- Block with 10% normal goat serum in PBS, 30min >- Wash PBSx3 >- Block with Streptavidin, 15min (Vector Streptavidin/Biotin blocking >kit) >- Wash PBS, 3x5min >- Block with Biotin, 15min >- Wash PBS, 3x5min >- Incubate with rat antiCD8 (BD Pahrmingen; diluted to ~5ug/ml), 2hr >(room temp) >- Wash PBSx3 >- Incubate with goat anti-rat:Biotin, 45min (Jackson; >biotin-SP-conjugated AffiniPure F(ab)2 fragment; product number >112-066-003; diluted 1/1000, ~1ug/ml in 10% normal mouse serum in PBS) >- Wash PBSx3 >- Apply peroxidase suppressor, 15min (Pierce) >- Wash PBSx3 >- Apply StreptABCcomplex, 45min (Vector) >- Wash PBSx3 >- Apply DAB, 2min (Sigma) >- Wash PBSx3 >- Wash tap water >- Counterstain with haematoxylin > >Thanks >Sonya > >-----Original Message----- >From: Andrea Hooper [mailto:anh2006@med.cornell.edu] >Sent: 19 February 2007 17:52 >To: Martin S. >Subject: Re: RE: [Histonet] Background in frozen pancreas sections >(continued...) > >I took this off Histonet for now .... > >How long were your incubations in avidin then biotin? Which kit >specifically did you use? How long were your washes? When I used the >streptavidin biotin blocking kit from Vector I found my background was >always worse and now only use avidin biotin blocking kits from Vector. >15 min in Avidin, 2 x 10 min washes, 15 min in biotin, 2 x 10 min >washes. > >As far as why you get background if you are using Jackson's mouse >adsorbed anti-rat ... I don't get it. How did you fix your samples? >Also, what concentration of primary, secondary and strep-HRP are you >using? What is the catalog # of the antibody from Jackson? > >I stain frozen pancreas all the time and get little background so I >want to help you figure this out :) > > > >----- Original Message ----- >From: "Martin S." >Date: Monday, February 19, 2007 11:31 am >Subject: RE: [Histonet] Background in frozen pancreas sections >(continued...) > >> >> Thanks Andrea, >> >> My goat anti-rat is from Jackson (F(ab)2, mouse adsorbed) but I find >> that if I don't dilute it in 10% normal mouse serum I get a lot of >> background - diluted in this way it seems clean on spleen sections >> but > >> maybe its different for pancreas. >> I used Vector's avidin/biotin blocking kit after serum block but >> beforeprimary - I will try this again concentrating on the washes. >> >> Thanks again >> Sonya >> >> >> >> -----Original Message----- >> From: Andrea Hooper [anh2006@med.cornell.edu] >> Sent: 19 February 2007 13:55 >> To: Martin S. >> Cc: histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] Background in frozen pancreas sections >> (continued...) >> >> It's likely your secondary (anti-rat IgG) is cross reacting with >> mouse > >> IgG in the tissue. I would suggest you purchase an anti-rat IgG >> which is highly cross adsorbed to many species INCLUDING mouse. This >> can be purchased from Jackson ImmunoResearch for example. >> >> Also, with pancreas you definitely need to do an AB block. You said >> it > >> increased background? That's odd and concerning ... how are you >> using the kit? Lots of washes? I perform my AB block specifically >> after serumblock and before primary (with loads of washing) and am >> able to get very little background. >> >> >> ----- Original Message ----- >> From: "Martin S." >> Date: Monday, February 19, 2007 5:28 am >> Subject: [Histonet] Background in frozen pancreas sections >> (continued...) >> >> > Sorry just thought some additional info might be useful; > > The >> pancreas tissue was frozen in OCT in a bath of isopentane on dry > >> ice, 10um sections were cut, air dried for 1hr, then fixed in >> acetone > for 10mins. I quenched endogenous peroxidase with Pierce >> Peroxidase > Suppressor. >> > Sections without primary or secondary antibodies still have a >> littel > background but its worse with the secondary (I have tried >> rabbit > anti-rat:Biotin and it was worse). >> > >> > Ta >> > >> > >> > >> > _______________________________________________ >> > Histonet mailing list >> > Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > >> >> >> >> >> >> > > > > > > >Content-Type: image/jpeg; > name="X10-1.jpg" >Content-Description: X10-1.jpg >Content-Disposition: attachment; > filename="X10-1.jpg" > >Attachment converted: Andrea's Mac:X10-1.jpg (JPEG/) (00CE6125) >Content-Type: image/jpeg; > name="X20-1.jpg" >Content-Description: X20-1.jpg >Content-Disposition: attachment; > filename="X20-1.jpg" > >Attachment converted: Andrea's Mac:X20-1.jpg (JPEG/) (00CE6126) >Content-Type: image/jpeg; > name="X20-2.jpg" >Content-Description: X20-2.jpg >Content-Disposition: attachment; > filename="X20-2.jpg" > >Attachment converted: Andrea's Mac:X20-2.jpg (JPEG/) (00CE6191) -- From Sandra.Harrison3 <@t> va.gov Fri Mar 2 08:53:12 2007 From: Sandra.Harrison3 <@t> va.gov (Harrison, Sandra C.) Date: Fri Mar 2 08:53:22 2007 Subject: [Histonet] Histology Job Opportunity in Minneapolis, MN Message-ID: <> Dear Histonetters, The Veteran's Admin. Medical Center in Minneapolis, MN., is seeking to fill a Histology vacancy. Great benefits & salary! The Histology lab is staffed by 6 congenial tech.'s and we range in experience level from those of us who have been in the field for 20+ years to 2 recent graduates. We don't have weekend call and do get 10 paid Holiday day's off per year. If you are interested, please go to the web link below. Human Resources will close this opening March 8, so please e-mail me (www.Sandra.Harrison3@VA.gov) or call 612-467-2449 if you need assistance wading thru the government application process. Sincerely, Sandy Shortcut to: http://jobsearch.usajobs.opm.gov/getjob.asp?JobID=54320393&AVSDM=2007%2D 02%2D27+08%3A49%3A33&Logo=0&q=Pathology+tech&sort=rv&FedEmp=N&vw=d&brd=3 876&ss=0&FedPub=Y&rad=25&zip=55417 From MElliott <@t> mrl.ubc.ca Fri Mar 2 09:12:36 2007 From: MElliott <@t> mrl.ubc.ca (Mark Elliott) Date: Fri Mar 2 09:14:00 2007 Subject: [Histonet] Region IX Education Day-Calgary June 8-9th Message-ID: <45E7CE64020000D600035B7E@mail.mrl.ubc.ca> Region IX Educational Day * Calgary, Alberta Region IX will be hosting an Education Day in Calgary, Alberta, June 8-9, 2007. Calgary is an attractive and dynamic city that is situated on the banks of the Bow River and a short driving distance from the beautiful Rocky Mountains. Calgary is famous for its annual stampede that attracts millions of spectators and some of the best rodeo champions in North America. Calgary is home of the NHL Calgary Flames and has hosted such events as the 1988 Winter Olympics. This beautiful city seemed the ideal setting for Region IX*s next Education event. The Education Day will be held at the Southern Alberta Institute of Technology (SAIT) on June 8 -9, 2007. This will be Region IX*s first time hosting a one and a half event and the Educational Committee members look forward to providing the participants with a list of high quality speakers and great topics. The Educational Committee is also hoping that this event will attract not only our Canadian histology colleagues but also our American neighbours. Visit our website (http://www.nshregionix.org) for more details for this event. The website will be updated on a regular basis as more information becomes available. (Click on Education button). We look forward to seeing everyone in Calgary. Mark Elliott Region IX Education Committee Chair ***CONFIDENTIALITY NOTICE*** This electronic message is intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From brett_connolly <@t> merck.com Fri Mar 2 09:16:17 2007 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Fri Mar 2 09:16:45 2007 Subject: [Histonet] Contract Lab doing FISH Message-ID: <355C35514FEAC9458F75947F5270974D016FA89C@usctmx1103.merck.com> Anybody know one? Brett M. Connolly, Ph.D. Research Fellow MRL, Imaging Research Merck & Co., Inc. WP-44K PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com ------------------------------------------------------------------------------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------------ From HornHV <@t> archildrens.org Fri Mar 2 09:23:09 2007 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Mar 2 09:23:34 2007 Subject: [Histonet] FISH Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB700E@EMAIL.archildrens.org> Are any of you performing FISH tests? Are they difficult to do? Time consuming? Expensive? Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From Sheri.Meilus <@t> va.gov Fri Mar 2 09:46:48 2007 From: Sheri.Meilus <@t> va.gov (Meilus, Sheri D.) Date: Fri Mar 2 09:50:30 2007 Subject: [Histonet] Online BS in Histotechnology Message-ID: Dear Histonetters, Thank you all for your responses to the survey question I put out on this subject. The good news, so far, is that I have received this response back from Rhoda Jost, who is the MLT/HT Program Director at Florida Community College Jacksonville: Sheri- I was able to have the 4 year HT degree put on the list of new programs to investigate. Could you forward the responses to the following people: nhenning@fccj.edu (Dr. Neal Henning, Dean of Workforce) and me rjost@fccj.edu Rhoda Jost MLT/HT Program Director Now, may I ask all of you to pass the word along to anyone in the Histology community that would be interested in this opportunity? Ask them to please email both Dr. Henning and Rhoda indicating their interest in this opportunity. They are putting the HT 4 year degree option on the list of new programs to INVESTIGATE and the chances of this becoming a reality will be based on parameters including number of HTs interested in such a program as well as shortages in staffing. So, if any of you who are supervisors, or even "working on the bench HTs" can provide any statistics on job openings, as well as difficulty recruiting due to shortage of Histotechs, that would be extremely helpful. I will be forwarding all your letters to Rhoda and Dr. Henning, but don't let that stop you from emailing them directly!! Thank you all, and good luck to all of us! S Sheri Meilus, HT(ASCP)QIHC Anatomic Pathology Supervisor Bay Pines VAMC Bay Pines, FL 33744 727-398-6661 4596 From ancillarypath <@t> mac.com Fri Mar 2 10:28:23 2007 From: ancillarypath <@t> mac.com (ancillarypath@mac.com) Date: Fri Mar 2 10:28:19 2007 Subject: [Histonet] Re: Contract Lab doing FISH In-Reply-To: <200703021532.l22FW2Hi014748@mac.com> References: <200703021532.l22FW2Hi014748@mac.com> Message-ID: <78E0C2CD-C442-4D15-9492-25BDAD0DCDAD@mac.com> Our laboratory does contract FISH and CISH work. Please contact us directly for details and fee schedules. ============================== Hadi Yaziji, M.D., Medical Director Vitro Molecular Laboratories, President, Ancillary Pathways 7000 SW 62nd Avenue, Suite Penthouse-C Miami, FL 33143 Tel 305.740.4440 Fax 786.513.0175 www.vitromolecular.com www.ancillarypath.com This email message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e- mail and destroy all copies of the original message. Date: Fri, 2 Mar 2007 10:16:17 -0500 From: "Connolly, Brett M" Subject: [Histonet] Contract Lab doing FISH To: histonet@lists.utsouthwestern.edu Message-ID: <355C35514FEAC9458F75947F5270974D016FA89C@usctmx1103.merck.com> Content-Type: text/plain; charset=us-ascii Anybody know one? Brett M. Connolly, Ph.D. Research Fellow MRL, Imaging Research Merck & Co., Inc. WP-44K PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com From teresa.p.wylie <@t> gsk.com Fri Mar 2 10:29:43 2007 From: teresa.p.wylie <@t> gsk.com (teresa.p.wylie@gsk.com) Date: Fri Mar 2 10:30:03 2007 Subject: [Histonet] Feedback on Leica 300 In-Reply-To: <200703021521.l22FLVtH020657@stvsfimr001.ggr.co.uk> Message-ID: We purchased 2 Leica ASP300 processors in 2001. We were pleased with the performance and the service we received, so in 2005 we purchased another one to replace our older VIP. We have had few problems over the years and some of those were technician errors. The programs are easy to use and I can even change the reagents without making a mess. Teresa Wylie, HT(ASCP) Histology Supervisor GlaxoSmithKline Research Triangle Park, NC 27709 ----- Original Message ----- Can anyone give me any comments about Leica ASP 300 tissue processor? it would be helpful in my decision to purchase this instrument. I would appreciate if vendors stay out of this and only users can give me their comments=2E Thank you very much. Reuel Cornelia Cellular Pathology Department Texas Scottish Rite Hospital Dallas, TX 75243 214-559-7766 From sbreeden <@t> nmda.nmsu.edu Fri Mar 2 11:22:46 2007 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri Mar 2 11:22:56 2007 Subject: [Histonet] Slide Retention SOP? Message-ID: <4D14F0FC9316DD41972D5F03C070908B8F4370@nmdamailsvr.nmda.ad.nmsu.edu> I know someone that's reading this already has an SOP that addresses the retention of original slides (and their recuts that may have been required for diagnostic purposes, i.e., deeper sections, special stains, etc.). I need to define the necessity of retaining the slides that were used to diagnose a case and define the difference between a recut for diagnostic purposes vs. a recut made for a pathologist's personal collection. I am adamant that the original slide(s) must be filed and not "swapped" for the recut in those permanent files. I've "googled" the subject from all sorts of different angles but am not finding what I need. Referrals to online resources will be happily researched as well. Thank you! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 From ana.merino-trigo <@t> wanadoo.fr Fri Mar 2 11:57:45 2007 From: ana.merino-trigo <@t> wanadoo.fr (Ana MERINO-TRIGO) Date: Fri Mar 2 11:57:54 2007 Subject: [Histonet] IHC screening on TMA slides Message-ID: <16856336.81091172858265135.JavaMail.www@wwinf1606> Hi Histonet, I was wondering if anyone could give me any ideas or suggestions in order to perform IHC screening over a TMA slide containing about 100 spots of 0.6 or 1.0 mm of the same tissue. The idea it will be to select hybridomes for further characterization based on IHC reactivity on a given positive control tissue. Around 100 hybridomas to test on a TMA slide containing spots from the same sample. I cannot see the way to do the immuno without spread the antibody in between the spots. I heard that exits multispot type of lids that you could put over the slide to perform the immno but I don't really know where to look for these lids or if they are good enough to be sure that antibody doesn't spread in between spots. Any information it will be really appreciate it, All the best, Ana From Sheri.Meilus <@t> va.gov Fri Mar 2 12:16:39 2007 From: Sheri.Meilus <@t> va.gov (Meilus, Sheri D.) Date: Fri Mar 2 12:17:08 2007 Subject: [Histonet] RE: Online BS in Histology In-Reply-To: <5mv6l3$1lmvru@mtares2.res.net.va.gov> References: <5mv6l3$1lmvru@mtares2.res.net.va.gov> Message-ID: Dear group, If any of you will be emailing Rhoda and Dr. Henning with letters of your interest in a program, would you please copy me on the emails. I'll keep them in a folder for future use in case FCCJ doesn't approve implementation of the program for some reason. I am dialoging with other entities and they can be of use in communication with other colleges/universities in the future if this doesn't go through. Thanks S Sheri Meilus, HT(ASCP)QIHC Anatomic Pathology Supervisor Bay Pines VAMC Bay Pines, FL 33744 727-398-6661 4596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, March 02, 2007 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 40, Issue 4 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Online BS in Histotechnology (Meilus, Sheri D.) 2. Re: Contract Lab doing FISH (ancillarypath@mac.com) 3. Feedback on Leica 300 (teresa.p.wylie@gsk.com) 4. Slide Retention SOP? (Breeden, Sara) 5. IHC screening on TMA slides (Ana MERINO-TRIGO) ---------------------------------------------------------------------- Message: 1 Date: Fri, 2 Mar 2007 10:46:48 -0500 From: "Meilus, Sheri D." Subject: [Histonet] Online BS in Histotechnology To: Message-ID: Content-Type: text/plain; charset="us-ascii" Dear Histonetters, Thank you all for your responses to the survey question I put out on this subject. The good news, so far, is that I have received this response back from Rhoda Jost, who is the MLT/HT Program Director at Florida Community College Jacksonville: Sheri- I was able to have the 4 year HT degree put on the list of new programs to investigate. Could you forward the responses to the following people: nhenning@fccj.edu (Dr. Neal Henning, Dean of Workforce) and me rjost@fccj.edu Rhoda Jost MLT/HT Program Director Now, may I ask all of you to pass the word along to anyone in the Histology community that would be interested in this opportunity? Ask them to please email both Dr. Henning and Rhoda indicating their interest in this opportunity. They are putting the HT 4 year degree option on the list of new programs to INVESTIGATE and the chances of this becoming a reality will be based on parameters including number of HTs interested in such a program as well as shortages in staffing. So, if any of you who are supervisors, or even "working on the bench HTs" can provide any statistics on job openings, as well as difficulty recruiting due to shortage of Histotechs, that would be extremely helpful. I will be forwarding all your letters to Rhoda and Dr. Henning, but don't let that stop you from emailing them directly!! Thank you all, and good luck to all of us! S Sheri Meilus, HT(ASCP)QIHC Anatomic Pathology Supervisor Bay Pines VAMC Bay Pines, FL 33744 727-398-6661 4596 ------------------------------ Message: 2 Date: Fri, 2 Mar 2007 11:28:23 -0500 From: ancillarypath@mac.com Subject: [Histonet] Re: Contract Lab doing FISH To: histonet@lists.utsouthwestern.edu Message-ID: <78E0C2CD-C442-4D15-9492-25BDAD0DCDAD@mac.com> Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed Our laboratory does contract FISH and CISH work. Please contact us directly for details and fee schedules. ============================== Hadi Yaziji, M.D., Medical Director Vitro Molecular Laboratories, President, Ancillary Pathways 7000 SW 62nd Avenue, Suite Penthouse-C Miami, FL 33143 Tel 305.740.4440 Fax 786.513.0175 www.vitromolecular.com www.ancillarypath.com This email message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e- mail and destroy all copies of the original message. Date: Fri, 2 Mar 2007 10:16:17 -0500 From: "Connolly, Brett M" Subject: [Histonet] Contract Lab doing FISH To: histonet@lists.utsouthwestern.edu Message-ID: <355C35514FEAC9458F75947F5270974D016FA89C@usctmx1103.merck.com> Content-Type: text/plain; charset=us-ascii Anybody know one? Brett M. Connolly, Ph.D. Research Fellow MRL, Imaging Research Merck & Co., Inc. WP-44K PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com ------------------------------ Message: 3 Date: Fri, 2 Mar 2007 11:29:43 -0500 From: teresa.p.wylie@gsk.com Subject: [Histonet] Feedback on Leica 300 To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii We purchased 2 Leica ASP300 processors in 2001. We were pleased with the performance and the service we received, so in 2005 we purchased another one to replace our older VIP. We have had few problems over the years and some of those were technician errors. The programs are easy to use and I can even change the reagents without making a mess. Teresa Wylie, HT(ASCP) Histology Supervisor GlaxoSmithKline Research Triangle Park, NC 27709 ----- Original Message ----- Can anyone give me any comments about Leica ASP 300 tissue processor? it would be helpful in my decision to purchase this instrument. I would appreciate if vendors stay out of this and only users can give me their comments=2E Thank you very much. Reuel Cornelia Cellular Pathology Department Texas Scottish Rite Hospital Dallas, TX 75243 214-559-7766 ------------------------------ Message: 4 Date: Fri, 2 Mar 2007 10:22:46 -0700 From: "Breeden, Sara" Subject: [Histonet] Slide Retention SOP? To: Message-ID: <4D14F0FC9316DD41972D5F03C070908B8F4370@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="us-ascii" I know someone that's reading this already has an SOP that addresses the retention of original slides (and their recuts that may have been required for diagnostic purposes, i.e., deeper sections, special stains, etc.). I need to define the necessity of retaining the slides that were used to diagnose a case and define the difference between a recut for diagnostic purposes vs. a recut made for a pathologist's personal collection. I am adamant that the original slide(s) must be filed and not "swapped" for the recut in those permanent files. I've "googled" the subject from all sorts of different angles but am not finding what I need. Referrals to online resources will be happily researched as well. Thank you! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 ------------------------------ Message: 5 Date: Fri, 2 Mar 2007 18:57:45 +0100 (CET) From: Ana MERINO-TRIGO Subject: [Histonet] IHC screening on TMA slides To: "histonet@lists.utsouthwestern.edu" Message-ID: <16856336.81091172858265135.JavaMail.www@wwinf1606> Content-Type: text/plain; charset=UTF-8 Hi Histonet, I was wondering if anyone could give me any ideas or suggestions in order to perform IHC screening over a TMA slide containing about 100 spots of 0.6 or 1.0 mm of the same tissue. The idea it will be to select hybridomes for further characterization based on IHC reactivity on a given positive control tissue. Around 100 hybridomas to test on a TMA slide containing spots from the same sample. I cannot see the way to do the immuno without spread the antibody in between the spots. I heard that exits multispot type of lids that you could put over the slide to perform the immno but I don't really know where to look for these lids or if they are good enough to be sure that antibody doesn't spread in between spots. Any information it will be really appreciate it, All the best, Ana ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 40, Issue 4 *************************************** From Jackie.O'Connor <@t> abbott.com Fri Mar 2 12:18:04 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Mar 2 12:18:41 2007 Subject: [Histonet] IHC screening on TMA slides In-Reply-To: <16856336.81091172858265135.JavaMail.www@wwinf1606> Message-ID: I don't understand why you would have a TMA with 100 cores of the same sample. Ana MERINO-TRIGO Sent by: histonet-bounces@lists.utsouthwestern.edu 03/02/2007 11:57 AM Please respond to ana.merino-trigo@wanadoo.fr To "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] IHC screening on TMA slides Hi Histonet, I was wondering if anyone could give me any ideas or suggestions in order to perform IHC screening over a TMA slide containing about 100 spots of 0.6 or 1.0 mm of the same tissue. The idea it will be to select hybridomes for further characterization based on IHC reactivity on a given positive control tissue. Around 100 hybridomas to test on a TMA slide containing spots from the same sample. I cannot see the way to do the immuno without spread the antibody in between the spots. I heard that exits multispot type of lids that you could put over the slide to perform the immno but I don't really know where to look for these lids or if they are good enough to be sure that antibody doesn't spread in between spots. Any information it will be really appreciate it, All the best, Ana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ana.merino-trigo <@t> wanadoo.fr Fri Mar 2 12:28:16 2007 From: ana.merino-trigo <@t> wanadoo.fr (Ana MERINO-TRIGO) Date: Fri Mar 2 12:28:26 2007 Subject: [Histonet] IHC screening on TMA slides Message-ID: <3396745.161001172860096736.JavaMail.www@wwinf1602> Because we do want to select in a quick way the right hybridoma in between more than 100 hybridomas using the same tissue. We thought in using the advantages of TMA technology, but the other way around. Same tissue in multiple spots to test different antibodies. So, we will need to find the way to separate physically the spots to perform different immunos in the same slide. Does this make sense? Thanks, Ana ----- Original Message ----- From: Jackie M O'Connor To: ana.merino-trigo@wanadoo.fr Cc: histonet@lists.utsouthwestern.edu ; histonet-bounces@lists.utsouthwestern.edu Sent: Friday, March 02, 2007 7:18 PM Subject: Re: [Histonet] IHC screening on TMA slides I don't understand why you would have a TMA with 100 cores of the same sample. Ana MERINO-TRIGO Sent by: histonet-bounces@lists.utsouthwestern.edu 03/02/2007 11:57 AM Please respond to ana.merino-trigo@wanadoo.fr To"histonet@lists.utsouthwestern.edu" cc Subject[Histonet] IHC screening on TMA slides Hi Histonet, I was wondering if anyone could give me any ideas or suggestions in order to perform IHC screening over a TMA slide containing about 100 spots of 0.6 or 1.0 mm of the same tissue. The idea it will be to select hybridomes for further characterization based on IHC reactivity on a given positive control tissue. Around 100 hybridomas to test on a TMA slide containing spots from the same sample. I cannot see the way to do the immuno without spread the antibody in between the spots. I heard that exits multispot type of lids that you could put over the slide to perform the immno but I don't really know where to look for these lids or if they are good enough to be sure that antibody doesn't spread in between spots. Any information it will be really appreciate it, All the best, Ana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.5.446 / Virus Database: 268.18.5/707 - Release Date: 01/03/2007 14:43 From pruegg <@t> ihctech.net Fri Mar 2 12:41:06 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Mar 2 12:41:18 2007 Subject: [Histonet] IHC screening on TMA slides In-Reply-To: <3396745.161001172860096736.JavaMail.www@wwinf1602> Message-ID: <00b001c75cfa$57664cd0$6501a8c0@Patsy> Physically separating 100 cores on one slide sounds like a nightmare to me. Even a very thin water barrier pen sounds tuff, but that is what I thought of. I would have thought you would have been better off making a tissue block with a small core of tissue and drop the small sections into 96 well elisa plates and is done for elisa/IHC. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ana MERINO-TRIGO Sent: Friday, March 02, 2007 11:28 AM To: Jackie M O'Connor Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC screening on TMA slides Because we do want to select in a quick way the right hybridoma in between more than 100 hybridomas using the same tissue. We thought in using the advantages of TMA technology, but the other way around. Same tissue in multiple spots to test different antibodies. So, we will need to find the way to separate physically the spots to perform different immunos in the same slide. Does this make sense? Thanks, Ana ----- Original Message ----- From: Jackie M O'Connor To: ana.merino-trigo@wanadoo.fr Cc: histonet@lists.utsouthwestern.edu ; histonet-bounces@lists.utsouthwestern.edu Sent: Friday, March 02, 2007 7:18 PM Subject: Re: [Histonet] IHC screening on TMA slides I don't understand why you would have a TMA with 100 cores of the same sample. Ana MERINO-TRIGO Sent by: histonet-bounces@lists.utsouthwestern.edu 03/02/2007 11:57 AM Please respond to ana.merino-trigo@wanadoo.fr To"histonet@lists.utsouthwestern.edu" cc Subject[Histonet] IHC screening on TMA slides Hi Histonet, I was wondering if anyone could give me any ideas or suggestions in order to perform IHC screening over a TMA slide containing about 100 spots of 0.6 or 1.0 mm of the same tissue. The idea it will be to select hybridomes for further characterization based on IHC reactivity on a given positive control tissue. Around 100 hybridomas to test on a TMA slide containing spots from the same sample. I cannot see the way to do the immuno without spread the antibody in between the spots. I heard that exits multispot type of lids that you could put over the slide to perform the immno but I don't really know where to look for these lids or if they are good enough to be sure that antibody doesn't spread in between spots. Any information it will be really appreciate it, All the best, Ana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.5.446 / Virus Database: 268.18.5/707 - Release Date: 01/03/2007 14:43 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Fri Mar 2 12:53:34 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Mar 2 12:54:28 2007 Subject: [Histonet] IHC screening on TMA slides In-Reply-To: <00b001c75cfa$57664cd0$6501a8c0@Patsy> Message-ID: It would make more sense to put 3-4 cores of multiple hybridomas on a tma slide resulting in 100 cores, and stain each tma slide with various antibodies to characterize your hybridomas. I do that for 100's of xenograft tumors. "Patsy Ruegg" 03/02/2007 12:41 PM To , "'Jackie M O'Connor'" cc , Subject RE: [Histonet] IHC screening on TMA slides Physically separating 100 cores on one slide sounds like a nightmare to me. Even a very thin water barrier pen sounds tuff, but that is what I thought of. I would have thought you would have been better off making a tissue block with a small core of tissue and drop the small sections into 96 well elisa plates and is done for elisa/IHC. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ana MERINO-TRIGO Sent: Friday, March 02, 2007 11:28 AM To: Jackie M O'Connor Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC screening on TMA slides Because we do want to select in a quick way the right hybridoma in between more than 100 hybridomas using the same tissue. We thought in using the advantages of TMA technology, but the other way around. Same tissue in multiple spots to test different antibodies. So, we will need to find the way to separate physically the spots to perform different immunos in the same slide. Does this make sense? Thanks, Ana ----- Original Message ----- From: Jackie M O'Connor To: ana.merino-trigo@wanadoo.fr Cc: histonet@lists.utsouthwestern.edu ; histonet-bounces@lists.utsouthwestern.edu Sent: Friday, March 02, 2007 7:18 PM Subject: Re: [Histonet] IHC screening on TMA slides I don't understand why you would have a TMA with 100 cores of the same sample. Ana MERINO-TRIGO Sent by: histonet-bounces@lists.utsouthwestern.edu 03/02/2007 11:57 AM Please respond to ana.merino-trigo@wanadoo.fr To"histonet@lists.utsouthwestern.edu" cc Subject[Histonet] IHC screening on TMA slides Hi Histonet, I was wondering if anyone could give me any ideas or suggestions in order to perform IHC screening over a TMA slide containing about 100 spots of 0.6 or 1.0 mm of the same tissue. The idea it will be to select hybridomes for further characterization based on IHC reactivity on a given positive control tissue. Around 100 hybridomas to test on a TMA slide containing spots from the same sample. I cannot see the way to do the immuno without spread the antibody in between the spots. I heard that exits multispot type of lids that you could put over the slide to perform the immno but I don't really know where to look for these lids or if they are good enough to be sure that antibody doesn't spread in between spots. Any information it will be really appreciate it, All the best, Ana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.5.446 / Virus Database: 268.18.5/707 - Release Date: 01/03/2007 14:43 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtarango <@t> nvcancer.org Fri Mar 2 12:54:40 2007 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Fri Mar 2 12:55:03 2007 Subject: [Histonet] Contract Lab doing FISH Message-ID: <5AEC610C1CE02945BD63A395BA763EDE1F99C3@NVCIEXCH02.NVCI.org> The Histology Core Lab here does FISH. Dr. David Ward, who invented FISH, works here as well. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Friday, March 02, 2007 7:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Contract Lab doing FISH Anybody know one? Brett M. Connolly, Ph.D. Research Fellow MRL, Imaging Research Merck & Co., Inc. WP-44K PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com ------------------------------------------------------------------------ ------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------ ------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From ploykasek <@t> phenopath.com Fri Mar 2 13:33:05 2007 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Fri Mar 2 13:33:31 2007 Subject: FW: [Histonet] Contract Lab doing FISH In-Reply-To: Message-ID: ------ Forwarded Message From: Patti Loykasek Date: Fri, 02 Mar 2007 07:23:56 -0800 To: "Connolly, Brett M" Subject: Re: [Histonet] Contract Lab doing FISH Hi Brett. We do many types of FISH on solid tumors and for leukemias/lymphomas. What probes are you looking for? Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > Anybody know one? > > Brett M. Connolly, Ph.D. > Research Fellow > MRL, Imaging Research > Merck & Co., Inc. > WP-44K > PO Box 4 > West Point, PA 19486 > PH 215-652-2501 > fax. 215-993-6803 > e-mail. brett_connolly@merck.com > > > ------------------------------------------------------------------------------ > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, > New Jersey, USA 08889), and/or its affiliates (which may be known > outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD > and in Japan, as Banyu - direct contact information for affiliates is > available at http://www.merck.com/contact/contacts.html) that may be > confidential, proprietary copyrighted and/or legally privileged. It is > intended solely for the use of the individual or entity named on this > message. If you are not the intended recipient, and have received this > message in error, please notify us immediately by reply e-mail and then > delete it from your system. > > ------------------------------------------------------------------------------ > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------ End of Forwarded Message ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From collette2 <@t> llnl.gov Fri Mar 2 13:52:02 2007 From: collette2 <@t> llnl.gov (Nicole Collette) Date: Fri Mar 2 13:52:10 2007 Subject: [Histonet] bone/cartilage sections falling off slides ISH Message-ID: <9d114fd0703021152uf0f6e3aobbedbec62bde82e@mail.gmail.com> Hello, I am relatively new to the land of histology (you can also read that as "self-taught"), and I am in a lab that is studying skeletal development. We are doing some histology stains and some fluorescent ISH on embryonic mouse limb sections, and are having trouble keeping the sections on the slides during processing. The tissues are embryonic mouse tissues, E14.6, E16.5, fixed in 4% PFA, embedded in paraffin using standard protocols. I have used charged slides and polylysine slides and in both cases, I float the ribbons in DEPC water/Ethanol and then move them to 39C water bath for putting on slides (brand new, accurate temperature, no water bath additives, clean DEPC water). I do not seem to have issues for the most part with the cartilage wrinkling up, which can sometimes be an issue. I dry the slides in vertical position O/N and then store @ -20C with dessicant. The sections stick just fine during regular staining (H&E, cartilage stains), and anything without bone sticks just fine during any processing (taken from the same embryos at the same time, embedded the same way), but the sections that contain only limbs fall right off. I am wondering if this is a mechanical issue related to doing steps in coplin jars with shaking as opposed to on individual horizontally situated slides, a processing issue pre-ISH, or some other handling issue that might be a simple case of "newbie-don't know". The tech I have doing the ISH does a relatively lengthy hot antigen-retrieval step at the beginning that I think may be the problem (they swear by it, but I think it's a bad idea for ISH), but she says even without that step, the sections don't fare well. The RNA quality is good, when we get sections that stick, the probes work well. The sections come off primarily starting from the middle of the long bone cavity working out toward the cartilage edges. I think there may also be an issue of the Prot K step, since their protocol is optimized for whole body sections at the same stage, so there is a much larger piece of tissue on the slide (we are working on doing control experiments for these options now). I just want to see if there is something else I can be doing to keep the slides together, I know that bone and cartilage are "exception" tissues, and different rules may apply when handling/processing. Any advice would be most gratefully appreciated. Thanks in advance for the resounding response! (and sorry about the lengthy description, wanted to provide as much info as possible) Sincerely, Nicole Collette From liz <@t> premierlab.com Fri Mar 2 14:19:29 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Mar 2 14:10:33 2007 Subject: [Histonet] bone/cartilage sections falling off slides ISH In-Reply-To: <9d114fd0703021152uf0f6e3aobbedbec62bde82e@mail.gmail.com> Message-ID: <000001c75d08$15571aa0$0d00a8c0@domain.Premier> Collette For bone and cartilage I would try to work with enzymatic retreival methods, such as proteinase K, pronase, trypsin, or hyaluronic acid. Not all antibodies will work with enzyme digestion but quite a few do, the enzyme retreival methods will not be as harsh as HEIR. If you do have to use HIER I would use a steamer or a gentler method. Lower temperatures with longer retreival periods might help, but I have been unable to get these to work well in my hands on bone. Pasty I think has had success with longer retrieval times at lower temps, maybe she can respond with some suggestions. I also use a good silane treated slide, and there are ones that are better than others. When I section I do not let bone sections air dry at room temp I place them on a warm hot plate (around 44 degrees) you don't want the paraffin to melt, I find that that sometimes causes the articular cartilage to flip. I leave then on the hot plate overnight and do not place them in an over afterwords. I get pretty good success with joint sections from mice, rats, dog, sheep, goat, etc. with this method. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicole Collette Sent: Friday, March 02, 2007 12:52 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] bone/cartilage sections falling off slides ISH Hello, I am relatively new to the land of histology (you can also read that as "self-taught"), and I am in a lab that is studying skeletal development. We are doing some histology stains and some fluorescent ISH on embryonic mouse limb sections, and are having trouble keeping the sections on the slides during processing. The tissues are embryonic mouse tissues, E14.6, E16.5, fixed in 4% PFA, embedded in paraffin using standard protocols. I have used charged slides and polylysine slides and in both cases, I float the ribbons in DEPC water/Ethanol and then move them to 39C water bath for putting on slides (brand new, accurate temperature, no water bath additives, clean DEPC water). I do not seem to have issues for the most part with the cartilage wrinkling up, which can sometimes be an issue. I dry the slides in vertical position O/N and then store @ -20C with dessicant. The sections stick just fine during regular staining (H&E, cartilage stains), and anything without bone sticks just fine during any processing (taken from the same embryos at the same time, embedded the same way), but the sections that contain only limbs fall right off. I am wondering if this is a mechanical issue related to doing steps in coplin jars with shaking as opposed to on individual horizontally situated slides, a processing issue pre-ISH, or some other handling issue that might be a simple case of "newbie-don't know". The tech I have doing the ISH does a relatively lengthy hot antigen-retrieval step at the beginning that I think may be the problem (they swear by it, but I think it's a bad idea for ISH), but she says even without that step, the sections don't fare well. The RNA quality is good, when we get sections that stick, the probes work well. The sections come off primarily starting from the middle of the long bone cavity working out toward the cartilage edges. I think there may also be an issue of the Prot K step, since their protocol is optimized for whole body sections at the same stage, so there is a much larger piece of tissue on the slide (we are working on doing control experiments for these options now). I just want to see if there is something else I can be doing to keep the slides together, I know that bone and cartilage are "exception" tissues, and different rules may apply when handling/processing. Any advice would be most gratefully appreciated. Thanks in advance for the resounding response! (and sorry about the lengthy description, wanted to provide as much info as possible) Sincerely, Nicole Collette _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Mary.Vaughan <@t> RoswellPark.org Fri Mar 2 15:50:41 2007 From: Mary.Vaughan <@t> RoswellPark.org (Vaughan, Mary) Date: Fri Mar 2 15:50:52 2007 Subject: [Histonet] IL-6 in human FFPE tissues Message-ID: <6FF91AE4F1DC7743A6466E334EB865AE0BE0F881@VERITY.roswellpark.org> Good afternoon everybody- Does anyone have any recommendation for a good IL-6 Ab for use in human FFPE tissues? Thanks, and have a nice weekend Best Regards, Mary Vaughan HT (ASCP) Research Specialist Roswell Park Cancer Institute ASB-212 Elm & Carlton Streets Buffalo, N Y 14263 phone: 716.845.3006 FAX: 716.845.3489 email: mary.vaughan@roswellpark.org This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. From liz <@t> premierlab.com Fri Mar 2 16:22:46 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Mar 2 16:13:44 2007 Subject: [Histonet] IL-6 in human FFPE tissues In-Reply-To: <6FF91AE4F1DC7743A6466E334EB865AE0BE0F881@VERITY.roswellpark.org> Message-ID: <000001c75d19$4dd21e50$0d00a8c0@domain.Premier> Mary I use the IL-6 from abcam it's a rabbit polyclonal, I have used it on both mouse and rat specimens and it works, I have not tried it on human tissue, but that's what the antibody was intended to stain for was human IL-6. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vaughan, Mary Sent: Friday, March 02, 2007 2:51 PM To: Histonet Subject: [Histonet] IL-6 in human FFPE tissues Good afternoon everybody- Does anyone have any recommendation for a good IL-6 Ab for use in human FFPE tissues? Thanks, and have a nice weekend Best Regards, Mary Vaughan HT (ASCP) Research Specialist Roswell Park Cancer Institute ASB-212 Elm & Carlton Streets Buffalo, N Y 14263 phone: 716.845.3006 FAX: 716.845.3489 email: mary.vaughan@roswellpark.org This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1971 (20070110) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From jnocito <@t> satx.rr.com Fri Mar 2 17:38:03 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Mar 2 17:38:24 2007 Subject: [Histonet] Purple Haze References: <005201c75c64$2c0d42a0$649eae18@yourxhtr8hvc4p> Message-ID: <004c01c75d23$d4afad70$649eae18@yourxhtr8hvc4p> Toe supreme with a light butter/garlic sauce with rice pilaf, toemaine salad (oops that Romaine salad) with a raspberry vinaigrette and orange sherbet for dessert. Speaking of ptomaine, I hope I don't get poisoned. Happy Friday ----- Original Message ----- From: "Edwards, R.E." To: "Joe Nocito" ; "Edwards, R.E." ; "Jason Wickersham" ; Sent: Friday, March 02, 2007 3:56 AM Subject: RE: [Histonet] Purple Haze So what's on todays menu?, "TOED IN THE HOLE" -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: 02 March 2007 00:46 To: Edwards, R.E.; Jason Wickersham; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Purple Haze Lunch, of course. ----- Original Message ----- From: "Edwards, R.E." To: "Jason Wickersham" ; Sent: Wednesday, February 28, 2007 9:36 AM Subject: RE: [Histonet] Purple Haze HEY JOE WHERE YOU GOING WITH THAT TOE IN YOUR HAND??. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jason Wickersham Sent: 28 February 2007 15:26 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Purple Haze ...Excuse me while I kiss this "slide"... Jason Wickersham Application Specialist Product Unit Electron Microscopy OLYMPUS SOFT IMAGING SOLUTIONS 84 E Grand Avenue Montvale, NJ 07645 Tel: 551-804-1845 Email: Jason.Wickersham@Olympus-SIS.com http://www.soft-imaging.net and http://www.olympus-sis.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, February 27, 2007 1:12 PM To: 'Fred Underwood'; lblazek@digestivespecialists.com; RJLevier@LancasterGeneral.org; ree3@leicester.ac.uk; histonet@lists.utsouthwestern.edu; jnocito@satx.rr.com; JWEEMS@sjha.org Subject: RE: [Histonet] Purple Haze Now you are talking! Hair Metal was/is great! Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fred Underwood Sent: Tuesday, February 27, 2007 8:12 AM To: lblazek@digestivespecialists.com; RJLevier@LancasterGeneral.org; ree3@leicester.ac.uk; histonet@lists.utsouthwestern.edu; jnocito@satx.rr.com; JWEEMS@sjha.org Subject: RE: [Histonet] Purple Haze How about a shout out to the "hair metal" days of the '80's. Does everyone remember Winger's version of Purple Haze. >>> "Blazek, Linda" 2/27/2007 7:18 AM >>> Prince was Purple Rain! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, February 26, 2007 9:26 PM To: Joe Nocito; Edwards, R.E.; LeVier, Rebecca J; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Purple Haze Poor Joe the Toe... Not Jimmy - Prince. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Monday, February 26, 2007 6:58 PM To: Edwards, R.E.; LeVier, Rebecca J; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Purple Haze is that a Jimmy Hendrix song? Seriously Becky, are you staining your H&Es with an automatic stainer? We were getting the same problem until we checked the flow of our rinse water. Someone with a sense of humor turned the wrong valve and instead of shutting the water off, they decreased the flow. JTT ----- Original Message ----- From: "Edwards, R.E." To: "LeVier, Rebecca J" ; Sent: Monday, February 26, 2007 9:20 AM Subject: RE: [Histonet] Purple Haze Well that would be a new experience to me. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LeVier, Rebecca J Sent: 26 February 2007 14:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Purple Haze Hi Folks, We have encountered an artifact which we call "purple haze" where the tissue looks as it waited too long to be fixed. It is not every piece of tissue in a cassette. I know that others have had this problem in the past and I was wondering what you have done to fix this. We also have had this problem and to fix it, we changed processors. We are now experiencing this problem again. If any one can help, I would really appreciate it. Thanks in advance! Becky Email: rjlevier@lancastergeneral.org Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. 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Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From karenadams <@t> comcast.net Fri Mar 2 17:52:21 2007 From: karenadams <@t> comcast.net (karenadams@comcast.net) Date: Fri Mar 2 17:52:33 2007 Subject: [Histonet] Re: North Carolina Meeting Message-ID: <030220072352.24973.45E8B8B500003B330000618D22007589429C030E0B0E020A9D0E05@comcast.net> Wondering if anyone is planning on attending the meeting in Durham in April. -- Karen Adams Pathology Laboratories West 9303 Park West Blvd Knoxville, TN 37923 (865) 690-2111 From lailasch <@t> aol.com Sat Mar 3 10:05:29 2007 From: lailasch <@t> aol.com (lailasch@aol.com) Date: Sat Mar 3 10:05:39 2007 Subject: [Histonet] renal needle biopsy size Message-ID: <8C92BC4E6435B6A-11B4-9A8A@FWM-D33.sysops.aol.com> I'm a histotech student who needs to simulate a proper sized renal needle biopsey. Any suggestions? ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From shroffsm <@t> vcu.edu Sat Mar 3 13:00:23 2007 From: shroffsm <@t> vcu.edu (Seema Shroff) Date: Sat Mar 3 13:00:34 2007 Subject: [Histonet] TUNEL assay validity issues Message-ID: Hi everyone, I am a grad student doing fluorescently labeled TUNEL assay on mouse spinal cord frozen sections. I used 10 day mouse liver as my positive control and I'm pretty sure that my technique works. But I have a question - The TUNEL enzyme does not distinguish between apoptotic DNA breaks and DNA physically broken (especially on the surfaces of the section) due to the cryostat knife slicing through the tissue block. The Roche technical support confirmed this. TUNEL is such a widely used technique and well-accepted. Can anyone shed light on this doubt? Is it really of concern and if yes, what can I do to validate this method? Thank you, Seema. From RSRICHMOND <@t> aol.com Sat Mar 3 15:07:46 2007 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Sat Mar 3 15:08:00 2007 Subject: [Histonet] Re: renal needle biopsy size Message-ID: Lailasch on AOL asks: >>I'm a histotech student who needs to simulate a proper sized renal needle biopsy. Any suggestions?<< Haven't seen one done since I was a medical student (you don't want to know how long ago that was!), but a quick Google of the topic suggests that a 15 gauge cutting needle (of the Tru-Cut type) is commonly used, with other needle sizes in fairly common use also. These are usually one-time use needles, and I would think you could get a discarded one fairly easily, though I've never tried. The areas of normal kidney in a nephrectomy specimen for cancer (alas, these are all too common) would be ideal, though autopsy material would also serve. You should suspend the specimen in saline in a Petri dish and look at it with a dissecting microscope, learning to identify the little red dots that are the glomeruli. This is something you'll be asked to do on a percutaneous needle biopsy specimen, before cutting it into separate pieces for electron microscopy (into glutaraldehyde), immunofluorescence (into Michel's or Zeus transport medium), and light microscopy (into neutral buffered formalin). If I were the on-call pathologist that day, I'd expect you to call me to look at the specimen, though it would be fine with me if you felt capable of handling it yourself. Histologists and pathologists really ought to make "pseudo-biopsy" specimens like this rather frequently - any time you're training somebody, or bringing a new technique online. Bob Richmond Samurai Pathologist Knoxville TN ************************************** AOL now offers free email to everyone. Find out more about what's free from AOL at http://www.aol.com. From RSRICHMOND <@t> aol.com Sat Mar 3 15:09:38 2007 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Sat Mar 3 15:09:54 2007 Subject: [Histonet] Re: Online BS in Histology Message-ID: Online BS in Histology - that's what Histonet is about, isn't it? Seriously, though, make sure we're all kept informed about this program - I can immediately think of two histotechnologists who'd be interested. Bob Richmond Samurai Pathologist Knoxville TN ************************************** AOL now offers free email to everyone. Find out more about what's free from AOL at http://www.aol.com. From langxingpan <@t> pantomics.com Sat Mar 3 15:51:42 2007 From: langxingpan <@t> pantomics.com (Langxing Pan) Date: Sat Mar 3 15:52:03 2007 Subject: [Histonet] RE: IHC screening on TMA slides Message-ID: <000c01c75dde$21c574e0$6f32b545@Master> Hi Ana, It is extremely hard to make the TMA for IHC screening of 100 hybridomas simultaneously on one slide. I agree with Patsy that ELISA/IHC(up to 380 wells or samples per plate) would be the solution for real high throughput. This technique has been used to screen hybridomas on cells. You may just adapt it for tissue fragments. I used to use multi-well slides for my hybridoma IHC screening. I put two tissues per well. Each slide contains 10 wells. That was done before the TMA technology. Erie Scientific (now sold as Thermo Scientific) still sells these slides (http://www.eriesci.com/diagnostic/excell_slides.aspx). However it is very hard and time-consuming to pick sections onto each well on the slide. We recently made a TMA for a company for antibody screening, which contained eight 5mm cores. Each 5mm core contained five tissues of 1 mm core size. I think that it is possible to make up to 20 cores of 1 ~ 1.5mm/per slide that are separated well enough for antibody screening, or 8 big cores/per slide. Each big core can contain up to nine 1mm tissue elements. If you need any help about similar TMA, do let me know. All the best, Langxing Pan, M.D., Ph.D. Pantomics, Inc. 457 Mariposa Street CA 94107, U.S.A. Direct line: 1-510-207-9788 Fax: 1-510-653-1227 www.pantomics.com Date: Fri, 2 Mar 2007 18:57:45 +0100 (CET) From: Ana MERINO-TRIGO Subject: [Histonet] IHC screening on TMA slides To: "histonet@lists.utsouthwestern.edu" Message-ID: <16856336.81091172858265135.JavaMail.www@wwinf1606> Content-Type: text/plain; charset=UTF-8 Hi Histonet, I was wondering if anyone could give me any ideas or suggestions in order to perform IHC screening over a TMA slide containing about 100 spots of 0.6 or 1.0 mm of the same tissue. The idea it will be to select hybridomes for further characterization based on IHC reactivity on a given positive control tissue. Around 100 hybridomas to test on a TMA slide containing spots from the same sample. I cannot see the way to do the immuno without spread the antibody in between the spots. I heard that exits multispot type of lids that you could put over the slide to perform the immno but I don't really know where to look for these lids or if they are good enough to be sure that antibody doesn't spread in between spots. Any information it will be really appreciate it, All the best, Ana From tilman.fuldatal <@t> web.de Sat Mar 3 17:16:07 2007 From: tilman.fuldatal <@t> web.de (Tilman Krieger) Date: Sat Mar 3 16:43:07 2007 Subject: [Histonet] Problems using Pap Pen Message-ID: <45EA01B7.5020100@web.de> Hello everybody, I tried to do some ICCs (DAB / Ni) but had some problems, obviously caused by using a Pap pen (Manufactured by Beckman Coulter) in a wrong way. All my tissues that were put on Superfrost-slides (Manufacturer: Menzel) looked really strange: only half of the tissue was coloued. It seemed as if something had faded into the tissue. I do not exactly know what it was and at which point of the ICC it happent, but I suppose it was the pap pen. Other tissues from the same organ (the same animal!) were correctly coloured - they were fixated on different slides (Manufacturer unknown). Any suggestions? Which distance do you keep between tissue and pap pen line? How long do you let it dry? Thanks in advance, Til MR From Eric <@t> ategra.com Fri Mar 2 20:08:39 2007 From: Eric <@t> ategra.com (Eric Dye (ext 223)) Date: Sun Mar 4 01:39:43 2007 Subject: [Histonet] Histology opportunities in your area- Can you help? (update3/2/07) Message-ID: Hi - Histonetters - I have both permanent and full time (permanent) J0BS for HistoTechs throughout the US. Are you still working as a HistoTech at ? These permanent and temporary HISTOTECH J0BS are being filled quickly, don't miss out on your dream position - callmetoday ----------------------------------- Temporary HISTOTECH JOBS : ----------------------------------- (Updated Mar 2 2007) 1.South Carolina - Bench - 3 months (starting in August) 2.Western Pennsylvania -Bench- 3-6 months- Starting NOW 3. Florida (East coast) bench- 6 weeks - Need Florida Licence Permanent HISTOTECH J0BS : (Updated Mar 2 2007) ------------------------------------------------------- New Permanent HISTOTECH J0BS Listed below ------------------------------------------------------- 1. Michigan (Ann Arbor Area) Histotech- bench- perm 2. Northern Ohio- Histotech - bench - perm 3. Eastern Pennsylvania - Histotech - Bench - perm 4.Florida( Tampa Bay area) Histotech- bench- perm 5.Massachusetts (Greater Boston Area) Histotech- Bench - perm 6.Massachusetts ( Greater Boston Area) Histotech Supervisor 7.Northern Ohio - Histotech - Bench- perm 8. Western Pennsylvania - Bench - perm 9. West Virginia - Bench- perm 10.Massachusetts (Greater Boston Area) Supervisor- perm - HOTHOTHOT!!! 11.Massachusetts (South Boston) - Bench -perm 12. Kentucky (Northwestern)- Bench - perm - 2 openings!! 13. Virginia (Close to North Carolina Border!!) bench- perm ------------------------------------ OTHER HISTOTECH J0BS I have available ---------------------------------- 01. Central Virginia- Histotech- perm 02. Southern California- Histotech- perm 03. Central Florida - Histotech with some MOHS experience - perm - Hot 04. Southeast South Dakota- Histotech - Must have ties to South Dakota- perm- Hot! 05. Hot! Eastern, Massachusetts Seeking Histotechs of all experience levels, Greeaaat paaaay & Great Location - Call Today - 15% Raise Guaranteed! 06.NEWJ0B! Southeast Florida- Histotech - perm - (Need Florida License) 07.Ohio (Southern) - perm - Bench Histotech ( 2 openings) 08.Ohio (Northern) perm- Bench 09.Ohio (Central) perm- Bench 10.Central Florida -perm- Histotech (Need Florida License) 11. Florida (Tampa Bay area) 12. Florida, West Coast - both temp & perm openings- Bench Histotech -Very-Hot 13. New York ( Syracuse area) - Bench Histotech- perm 14. New York City (Long Island) - Bench- perm 15. Las Vegas - Bench Histotech- perm 16. Wisconsin- Histology Supervisor- BrandNew -Hot Hot Hot 17. Central-Illinois-Bench-Dermpath- BrandNew -Hot 18. Northern California- Bench- Routine Histology- BrandNew -------------------------- end list of Histotech Opportunities ------------------------------------- If you are interested in any of the HISTOTECH J0BS listed above - please call me at 800-466-9919 ext 223 or cell - 407-756-5507. Thank you, Eric Dye-Sr Allied Healthcare Recruiter 800-466-9919 ext 223 or Cell - 407-756-5507 P.S.: Feel free to pass along this email and my phone number to anyone who you think might be interested. P.S.S.: The clients are currently interviewing - and the positions will close soon - so if you are interested, please call me today at 1-800-466-9919 ext 229 --------------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing 800-466-9919 ext 223 - voice 407 671 6075 - fax eric@ategra.com To Learn More About Ategra: http://www.ategra.com ---------------------------------------------------------------- If you received this by mistake, or if you wish not to hear from me, please shoot me a mail to let me know and I'll not mail you again. ---------------------------------------------------------------- ---------------------------------------------------------------- Note: this message is intended for: Histonetters at histonet@lists.utsouthwestern.edu ---------------------------------------------------------------- From rjbuesa <@t> yahoo.com Sun Mar 4 08:16:14 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Mar 4 08:16:19 2007 Subject: [Histonet] Problems using Pap Pen In-Reply-To: <45EA01B7.5020100@web.de> Message-ID: <312596.9410.qm@web61216.mail.yahoo.com> Try using a simple wax pencil instead of a PAP pen. Absolutely innocuous! Ren? J. Tilman Krieger wrote: Hello everybody, I tried to do some ICCs (DAB / Ni) but had some problems, obviously caused by using a Pap pen (Manufactured by Beckman Coulter) in a wrong way. All my tissues that were put on Superfrost-slides (Manufacturer: Menzel) looked really strange: only half of the tissue was coloued. It seemed as if something had faded into the tissue. I do not exactly know what it was and at which point of the ICC it happent, but I suppose it was the pap pen. Other tissues from the same organ (the same animal!) were correctly coloured - they were fixated on different slides (Manufacturer unknown). Any suggestions? Which distance do you keep between tissue and pap pen line? How long do you let it dry? Thanks in advance, Til MR _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss an email again! Yahoo! Toolbar alerts you the instant new Mail arrives. Check it out. From koellingr <@t> comcast.net Sun Mar 4 23:29:09 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Sun Mar 4 23:29:16 2007 Subject: [Histonet] TUNEL assay validity issues Message-ID: <030520070529.17637.45EBAAA5000294FC000044E522135396539D09020704040A0105@comcast.net> Seema, I agree that you cannot necessarily distinguish an apoptotic from necrotic event from another DNA damaging event and be 100% confident in the TUNEL readout. DNA breaks are DNA breaks and they are there for any number of molecular reasons. TUNEL isn't and can't be a gold standard. On the other hand TUNEL, taken with a bit of common sense and with other observations can I think be a useful tool. If you are looking at mouse spinal cord and seeing what appear to be some TUNEL positive events, what are the possibilities? Could it truly be an apoptotic event? Sure, depending on your mouse model and study. Might it be necrotic? Sure but do you see any other evidence of necrosis around there? Might it be procedurally induced from the surface of a section? Sure but then why are 10 nuclei staining and the 100's around them, which are also on a cut surface, not staining? Also remember that not all apoptosis follows a single canonical molecular pathway. TUNEL or activated caspace-3 positivity is simply not ultimately pathognomonic of an apoptotic event. It would be like relying 100% on a single antibody to make a 100% definitive diagnosis on a tumor when in fact a panel of several antibodies is necessary to create a sufficient weight of evidence to raise your confidence to the highest level. Ray Almost, but not quite yet, employed in Seattle, WA -------------- Original message -------------- From: "Seema Shroff" > Hi everyone, > > > > I am a grad student doing fluorescently labeled TUNEL assay on mouse spinal > cord frozen sections. I used 10 day mouse liver as my positive control and > I'm pretty sure that my technique works. But I have a question - The TUNEL > enzyme does not distinguish between apoptotic DNA breaks and DNA physically > broken (especially on the surfaces of the section) due to the cryostat knife > slicing through the tissue block. > > > > The Roche technical support confirmed this. TUNEL is such a widely used > technique and well-accepted. Can anyone shed light on this doubt? Is it > really of concern and if yes, what can I do to validate this method? > > > > Thank you, > > > > Seema. > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From koellingr <@t> comcast.net Sun Mar 4 23:44:45 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Sun Mar 4 23:44:50 2007 Subject: [Histonet] IHC screening on TMA slides Message-ID: <030520070544.27758.45EBAE4D0005B67400006C6E22135396539D09020704040A0105@comcast.net> Ana, I thought about and briefly attempted to do something like this also when screening hybridoma's. But I could never get past a philosophical type argument. When I've screened for clones via FACS or ELISA or Western, I was always looking for a clone that had specificity for a restricted target. It was on or off. Hit or miss. There was just target there. But for tissue, besides your putative real target, there is a milieu of other tissue types and cells in tissue sections which can provide a great deal of information when screening uncharacterized supernatants. You might see something very unexpected with a clone, against some other target that you would miss with TMA sized bits of tissue. I have come across many surprises and useful info simply by seeing one clone on a large about of TMA's or tissue sections. I would have missed those discoveries with .6 mm diameter tissue sections. Could never have assessed the stickiness or dirtiness of any antibodies for tissues. If you are looking at hundreds of supes, I'm assuming these have not even been sub-cloned yet unless you have some very energetic hybridoma people. I think the idea of high throughput screening by applying 1 supe to one single .6 mm tissue section is indeed enticing, but I think it is hard to justify the amount of information you could possibly loose or never discover. Ray Almost, but not quite yet, employed in Seattle, WA -------------- Original message -------------- From: Ana MERINO-TRIGO > Because we do want to select in a quick way the right hybridoma in between more > than 100 hybridomas using the same tissue. We thought in using the advantages of > TMA technology, but the other way around. Same tissue in multiple spots to test > different antibodies. So, we will need to find the way to separate physically > the spots to perform different immunos in the same slide. Does this make sense? > Thanks, > Ana > ----- Original Message ----- > From: Jackie M O'Connor > To: ana.merino-trigo@wanadoo.fr > Cc: histonet@lists.utsouthwestern.edu ; > histonet-bounces@lists.utsouthwestern.edu > Sent: Friday, March 02, 2007 7:18 PM > Subject: Re: [Histonet] IHC screening on TMA slides > > > > I don't understand why you would have a TMA with 100 cores of the same sample. > > > > > Ana MERINO-TRIGO > Sent by: histonet-bounces@lists.utsouthwestern.edu > 03/02/2007 11:57 AM Please respond to > ana.merino-trigo@wanadoo.fr > > To"histonet@lists.utsouthwestern.edu" > cc > Subject[Histonet] IHC screening on TMA slides > > > > > > > > > Hi Histonet, > > I was wondering if anyone could give me any ideas or suggestions in order to > perform IHC screening over a TMA slide containing about 100 spots of 0.6 or 1.0 > mm of the same tissue. The idea it will be to select hybridomes for further > characterization based on IHC reactivity on a given positive control tissue. > Around 100 hybridomas to test on a TMA slide containing spots from the same > sample. I cannot see the way to do the immuno without spread the antibody in > between the spots. I heard that exits multispot type of lids that you could put > over the slide to perform the immno but I don't really know where to look for > these lids or if they are good enough to be sure that antibody doesn't spread in > between spots. > > Any information it will be really appreciate it, > > All the best, > Ana > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.5.446 / Virus Database: 268.18.5/707 - Release Date: 01/03/2007 14:43 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Mon Mar 5 04:10:48 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Mon Mar 5 04:11:10 2007 Subject: [Histonet] Purple Haze In-Reply-To: <004c01c75d23$d4afad70$649eae18@yourxhtr8hvc4p> References: <004c01c75d23$d4afad70$649eae18@yourxhtr8hvc4p> Message-ID: You forgot to mention the unforgettable game toerrine, the spicy chicken toekka and the unbelievable pate de toe gras to the sound of that well known ditty "Toe a deer, a hoof is a nail" -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: 02 March 2007 23:38 To: Edwards, R.E.; Jason Wickersham; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Purple Haze Toe supreme with a light butter/garlic sauce with rice pilaf, toemaine salad (oops that Romaine salad) with a raspberry vinaigrette and orange sherbet for dessert. Speaking of ptomaine, I hope I don't get poisoned. Happy Friday ----- Original Message ----- From: "Edwards, R.E." To: "Joe Nocito" ; "Edwards, R.E." ; "Jason Wickersham" ; Sent: Friday, March 02, 2007 3:56 AM Subject: RE: [Histonet] Purple Haze So what's on todays menu?, "TOED IN THE HOLE" -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: 02 March 2007 00:46 To: Edwards, R.E.; Jason Wickersham; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Purple Haze Lunch, of course. ----- Original Message ----- From: "Edwards, R.E." To: "Jason Wickersham" ; Sent: Wednesday, February 28, 2007 9:36 AM Subject: RE: [Histonet] Purple Haze HEY JOE WHERE YOU GOING WITH THAT TOE IN YOUR HAND??. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jason Wickersham Sent: 28 February 2007 15:26 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Purple Haze ...Excuse me while I kiss this "slide"... Jason Wickersham Application Specialist Product Unit Electron Microscopy OLYMPUS SOFT IMAGING SOLUTIONS 84 E Grand Avenue Montvale, NJ 07645 Tel: 551-804-1845 Email: Jason.Wickersham@Olympus-SIS.com http://www.soft-imaging.net and http://www.olympus-sis.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, February 27, 2007 1:12 PM To: 'Fred Underwood'; lblazek@digestivespecialists.com; RJLevier@LancasterGeneral.org; ree3@leicester.ac.uk; histonet@lists.utsouthwestern.edu; jnocito@satx.rr.com; JWEEMS@sjha.org Subject: RE: [Histonet] Purple Haze Now you are talking! Hair Metal was/is great! Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fred Underwood Sent: Tuesday, February 27, 2007 8:12 AM To: lblazek@digestivespecialists.com; RJLevier@LancasterGeneral.org; ree3@leicester.ac.uk; histonet@lists.utsouthwestern.edu; jnocito@satx.rr.com; JWEEMS@sjha.org Subject: RE: [Histonet] Purple Haze How about a shout out to the "hair metal" days of the '80's. Does everyone remember Winger's version of Purple Haze. >>> "Blazek, Linda" 2/27/2007 7:18 AM >>> Prince was Purple Rain! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, February 26, 2007 9:26 PM To: Joe Nocito; Edwards, R.E.; LeVier, Rebecca J; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Purple Haze Poor Joe the Toe... Not Jimmy - Prince. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Monday, February 26, 2007 6:58 PM To: Edwards, R.E.; LeVier, Rebecca J; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Purple Haze is that a Jimmy Hendrix song? Seriously Becky, are you staining your H&Es with an automatic stainer? We were getting the same problem until we checked the flow of our rinse water. Someone with a sense of humor turned the wrong valve and instead of shutting the water off, they decreased the flow. JTT ----- Original Message ----- From: "Edwards, R.E." To: "LeVier, Rebecca J" ; Sent: Monday, February 26, 2007 9:20 AM Subject: RE: [Histonet] Purple Haze Well that would be a new experience to me. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LeVier, Rebecca J Sent: 26 February 2007 14:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Purple Haze Hi Folks, We have encountered an artifact which we call "purple haze" where the tissue looks as it waited too long to be fixed. It is not every piece of tissue in a cassette. I know that others have had this problem in the past and I was wondering what you have done to fix this. We also have had this problem and to fix it, we changed processors. We are now experiencing this problem again. If any one can help, I would really appreciate it. Thanks in advance! Becky Email: rjlevier@lancastergeneral.org Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mmarti <@t> cmrb.eu Mon Mar 5 05:58:08 2007 From: mmarti <@t> cmrb.eu (=?iso-8859-1?Q?Marti_Gaudes=2C_Merc=E8?=) Date: Mon Mar 5 05:57:49 2007 Subject: [Histonet] BrdU and Dapi staining Message-ID: Dear all Do you know how can I improve the BrdU detection without lose the Dapi staining? I'm using 2N HCl 37?C 30 min. Thanks a lot! Merc? Mart? Gaudes From Janet.Keeping <@t> cna.nl.ca Mon Mar 5 06:05:53 2007 From: Janet.Keeping <@t> cna.nl.ca (Keeping, Janet) Date: Mon Mar 5 06:06:15 2007 Subject: [Histonet] Re: renal needle biopsy size In-Reply-To: References: Message-ID: <97B7B410F3436449B254A3F966EC7E1806563D15@exchange.cna.nl.ca> I teach histology and to provide a simulation of a renal biopsy I use a fixed kidney. I "stab" the kidney through the medulla and into the cortex with a glass Pasteur pipette. I use a piece of wire to push the 'core" back through the opening. It's kind of crude I guess but it serves its purpose. Janet -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of RSRICHMOND@aol.com Sent: March 3, 2007 5:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: renal needle biopsy size Lailasch on AOL asks: >>I'm a histotech student who needs to simulate a proper sized renal needle biopsy. Any suggestions?<< Haven't seen one done since I was a medical student (you don't want to know how long ago that was!), but a quick Google of the topic suggests that a 15 gauge cutting needle (of the Tru-Cut type) is commonly used, with other needle sizes in fairly common use also. These are usually one-time use needles, and I would think you could get a discarded one fairly easily, though I've never tried. The areas of normal kidney in a nephrectomy specimen for cancer (alas, these are all too common) would be ideal, though autopsy material would also serve. You should suspend the specimen in saline in a Petri dish and look at it with a dissecting microscope, learning to identify the little red dots that are the glomeruli. This is something you'll be asked to do on a percutaneous needle biopsy specimen, before cutting it into separate pieces for electron microscopy (into glutaraldehyde), immunofluorescence (into Michel's or Zeus transport medium), and light microscopy (into neutral buffered formalin). If I were the on-call pathologist that day, I'd expect you to call me to look at the specimen, though it would be fine with me if you felt capable of handling it yourself. Histologists and pathologists really ought to make "pseudo-biopsy" specimens like this rather frequently - any time you're training somebody, or bringing a new technique online. Bob Richmond Samurai Pathologist Knoxville TN ************************************** AOL now offers free email to everyone. Find out more about what's free from AOL at http://www.aol.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jo-ann.bader <@t> mcgill.ca Mon Mar 5 08:02:00 2007 From: jo-ann.bader <@t> mcgill.ca (Jo-Ann Bader, Ms.) Date: Mon Mar 5 08:03:33 2007 Subject: [Histonet] Collagen Staining Message-ID: Good Morning Everyone, I am looking for a stain for Collagen without a background stain. I have tried the Aniline blue from the Masson's Trichrome with unsatisfactory results. Jo-Ann Bader From histology.bc <@t> shaw.ca Mon Mar 5 09:05:20 2007 From: histology.bc <@t> shaw.ca (Paul Bradbury) Date: Mon Mar 5 09:06:23 2007 Subject: [Histonet] Collagen Staining In-Reply-To: References: Message-ID: <45EC31B0.20100@shaw.ca> Trying to stain collagen by using just the aniline blue solution from a trichrome method will not work. An acidified solution of aniline blue will non-selectively stain all acidophilic tissues (red cells, muscle, cytoplasm, collagen, etc.). The reason that the trichrome methods distinguish collagen from other tissue structures is that red cells and muscle have been pretreated with phosphomolybdic acid prior to staining in aniline blue. The presence of the aniline blue in those tissues inhibits (for a period of time) the attachment of the blue dye. The principles of the trichrome techniques depend on the molecular size of the dye molecules and the relative porosity of the tissue proteins that are to be stained. To selectively stain collagen without any other background staining, I would suggest trying the following. Bring the sections down to water as usual. Treat the sections in 1% aqeous phosphomolybdic acid for 5 minutes Drain off the acid. Do not wash as this will extract the PMA from the tissues. Stain in acidified aniline blue (or any one of the blue or green stain solutions used in a trichrome method) for 3 minutes. Rinse very briefly in distilled water. Just a few seconds, no longer. Dehydrate quickly through graded alcohols. Clear in xylene and mount. What I believe will happen is that the PMA will block entry of the dye to all tissue structures except collagen, thus giving selective coloration of the collagen fibres. Paul Bradbury Kamloops, BC Canada Jo-Ann Bader, Ms. wrote: >Good Morning Everyone, > >I am looking for a stain for Collagen without a background stain. I have tried the Aniline blue from the Masson's Trichrome with unsatisfactory results. > >Jo-Ann Bader > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > From gu.lang <@t> gmx.at Mon Mar 5 09:06:38 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Mon Mar 5 09:06:29 2007 Subject: AW: [Histonet] Collagen Staining In-Reply-To: Message-ID: <000c01c75f37$e14feca0$6412a8c0@dielangs.at> Try to mordant the stain with 5% Phosphormolybdenacid or Phosphortungstenacid for 10 min before Anilinblue (3-5min). The PMA should block Cytoplasma staining of Anilinblue. If it isn't sufficient prolong the incubation time. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Jo-Ann Bader, Ms. Gesendet: Montag, 05. M?rz 2007 15:02 An: Histonet@lists.utsouthwestern.edu Betreff: [Histonet] Collagen Staining Good Morning Everyone, I am looking for a stain for Collagen without a background stain. I have tried the Aniline blue from the Masson's Trichrome with unsatisfactory results. Jo-Ann Bader _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AGrobe2555 <@t> aol.com Mon Mar 5 11:48:17 2007 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Mon Mar 5 11:48:31 2007 Subject: [Histonet] GAG Stain Message-ID: Hello All, I'm trying to find a staining protocol for Gycosaminoglycans (GAGs). Does anyone have a protocol that will work on fixed sheep tissues? Thanks, Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital


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AOL now offers free email to everyone. Find out more about what's free from AOL at http://www.aol.com. From mcauliff <@t> umdnj.edu Mon Mar 5 12:13:22 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Mon Mar 5 12:12:57 2007 Subject: [Histonet] GAG Stain In-Reply-To: References: Message-ID: <45EC5DC2.9050508@umdnj.edu> Alcian Blue at pH 2.5 or 1.0, precceded by digestion with specific enzymes, will work for formalin-fixed (better is formalin-alcohol-acetic acid) tissues. There is also the "critical electrolyte concentration" methods with Alcian Blue. Some lectin methods (see Vector Labs website) will demo specific amino sugars of the GAG. Geoff AGrobe2555@aol.com wrote: >Hello All, >I'm trying to find a staining protocol for Gycosaminoglycans (GAGs). Does >anyone have a protocol that will work on fixed sheep tissues? >Thanks, >Albert > >Albert C. Grobe, PhD >International Heart Institute of Montana Foundation >Tissue Engineering Lab, Saint Patrick Hospital > > >


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AOL now offers free >email to everyone. Find out more about what's free from AOL at >http://www.aol.com. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From POWELL_SA <@t> Mercer.edu Mon Mar 5 13:18:13 2007 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Mon Mar 5 13:18:53 2007 Subject: [Histonet] GSH Meeting Message-ID: <01MDV2E1E6OW8WZ257@Macon2.Mercer.edu> Good news Guys, I have learned that the Mountain Creek Inn at Callaway will still honor our rate of $119 single, double, triple or quad as long as they have rooms available. This is their peak season and the rooms will not be around long. So if you did not meet the previous deadline of March 1st for reservations, call now before all the rooms are gone. Go to our website at www.histosearch.com/gsh for the complete program, hotel information and vendor information. The meeting registration deadline is April 1st to avoid a late fee. See you at Callaway. Shirley Powell GSH Secretary/Registrar From histology <@t> gradymem.org Mon Mar 5 14:07:53 2007 From: histology <@t> gradymem.org (histology@gradymem.org) Date: Mon Mar 5 14:08:04 2007 Subject: [Histonet] Oklahoma Society of Histotechnology Spring Meeting Message-ID: If you are close enough to attend, we would love you to join us in Norman, OK for our one day OSH Spring meeting. Read all about it on our website. Go to www.okhisto.org, click on the "Meetings" link to see the details. Angie Barnett, HTL(ASCP) Grady Memorial Hospital Pathology Department 405/224-2258 histology@gradymem.org From cervantes <@t> bendres.com Mon Mar 5 14:30:03 2007 From: cervantes <@t> bendres.com (Cervantes, Jessica) Date: Mon Mar 5 14:30:16 2007 Subject: [Histonet] Is mucus layer preservation possible? Message-ID: <8943D65F9AD70E4488AD6DE09F15088768C3AC@BRIEX04A> Hi - I am relatively new to the histology world, and have a project in which we'd like to image the mucus layer of the intestine. We have sections of rat intestinal tissue that we have embedded in OCT. The hope was that the thin layer of mucus that coats the tissue would be preserved by limiting the handling of the tissue and freezing it as quickly as possible in the OCT. We did not test to see whether the OCT would dissolve or perturb the mucus layer. The sections we have cut (using a cryostat) do not show the thin layer of mucus we hoped to see. PAS/Alcian Blue staining showed mucus in goblet cells, but none on the surface. My question is if the hope of preserving that thin mucus layer is completely foolish? And if not, what procedures should we have followed (assuming what we did destroyed the layer)? It is unclear from the literature what exactly the thickness of the layer would be in a rat, but I am assuming we are not seeing it because it's just not there (not that it's too thin). Any help or advice would be much appreciated. Thanks, Jessica Cervantes Bend Research, Inc From am3309 <@t> uga.edu Mon Mar 5 15:33:44 2007 From: am3309 <@t> uga.edu (Abigail M. Butler) Date: Mon Mar 5 15:33:57 2007 Subject: [Histonet] QIHC Message-ID: <20070305163344.AQP86948@punts1.cc.uga.edu> just curious to know if the QIHC is a hard test to take.... Abbie Butler, HT (ASCP) University of Georgia College of Veterinary Medicine From AnthonyH <@t> chw.edu.au Mon Mar 5 16:27:11 2007 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Mar 5 16:27:22 2007 Subject: [Histonet] Collagen Staining Message-ID: Try a Van Gieson's stain. To remove the yellow picric acid staining, rinse the slides in water before DC&M. Depending on choice of dye, you can get red, blue and even green collagen staining. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 > Bandaged Bear Day - Friday 30th March 2007 > For more information and to order merchandise visit www.bandagedbearday.com.au > > -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jo-Ann Bader, Ms. Sent: Tuesday, 6 March 2007 1:02 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Collagen Staining Good Morning Everyone, I am looking for a stain for Collagen without a background stain. I have tried the Aniline blue from the Masson's Trichrome with unsatisfactory results. Jo-Ann Bader _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From histology.bc <@t> shaw.ca Mon Mar 5 19:05:09 2007 From: histology.bc <@t> shaw.ca (Paul Bradbury) Date: Mon Mar 5 19:05:36 2007 Subject: [Histonet] Collagen Staining In-Reply-To: References: <45EC31B0.20100@shaw.ca> Message-ID: <45ECBE45.6030408@shaw.ca> Hi Jo-Ann, I think you are working in the right direction, you just need to be a bit more aggressive with the heteropolyacids to avoid any non-collagen staining. A number of years ago, I did quite a lot of work on the effects of various heteropolyacids on trichrome and fibrin stains. Phosphomolybdic acid is the most commonly used heteropolyacid, but there are several others with similar properties. Most labs have some phosphotungstic acid on hand. Some of the others I worked with were borotungstic acid and silicotungstic acid. I would be tempted to first try pre-treating the sections with 5% phosphotungstic acid for 5-10 minutes and incorporating 5% PTA in the stain solution as well. No washing between the two solutions. Can you tweak the imaging program or the illumination to over-ride very pale reactions? I am keeping my fingers crossed, Paul >Hi Paul, > >I guess I should have been a little more descriptive. That is exactly what I did. The problem is the background retains enough of a blue tinge to interfere with the imaging program they are using. > >Jo-Ann Bader > > > >-----Original Message----- >From: Paul Bradbury [mailto:histology.bc@shaw.ca] >Sent: Mon 3/5/2007 10:05 AM >To: Jo-Ann Bader, Ms.; HistoNet Server >Subject: Re: [Histonet] Collagen Staining > >Trying to stain collagen by using just the aniline blue solution from a >trichrome method will not work. An acidified solution of aniline blue >will non-selectively stain all acidophilic tissues (red cells, muscle, >cytoplasm, collagen, etc.). >The reason that the trichrome methods distinguish collagen from other >tissue structures is that red cells and muscle have been pretreated with >phosphomolybdic acid prior to staining in aniline blue. The presence of >the aniline blue in those tissues inhibits (for a period of time) the >attachment of the blue dye. The principles of the trichrome techniques >depend on the molecular size of the dye molecules and the relative >porosity of the tissue proteins that are to be stained. > >To selectively stain collagen without any other background staining, I >would suggest trying the following. > >Bring the sections down to water as usual. >Treat the sections in 1% aqeous phosphomolybdic acid for 5 minutes >Drain off the acid. Do not wash as this will extract the PMA from the >tissues. >Stain in acidified aniline blue (or any one of the blue or green stain >solutions used in a trichrome method) for 3 minutes. >Rinse very briefly in distilled water. Just a few seconds, no longer. >Dehydrate quickly through graded alcohols. >Clear in xylene and mount. > >What I believe will happen is that the PMA will block entry of the dye >to all tissue structures except collagen, thus giving selective >coloration of the collagen fibres. > >Paul Bradbury >Kamloops, BC >Canada > > > >Jo-Ann Bader, Ms. wrote: > > > >>Good Morning Everyone, >> >>I am looking for a stain for Collagen without a background stain. I have tried the Aniline blue from the Masson's Trichrome with unsatisfactory results. >> >>Jo-Ann Bader >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> >> > > > > > > > From HoustonR <@t> chi.osu.edu Tue Mar 6 07:42:44 2007 From: HoustonR <@t> chi.osu.edu (Houston, Ronald) Date: Tue Mar 6 07:43:11 2007 Subject: [Histonet] QIHC Message-ID: <979FF5962E234F45B06CF0DB7C1AABB20D77D030@chi2k3ms01.columbuschildrens.net> Embarrassingly simple Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Columbus Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 houstonr@chi.osu.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Abigail M. Butler Sent: Monday, March 05, 2007 4:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] QIHC just curious to know if the QIHC is a hard test to take.... Abbie Butler, HT (ASCP) University of Georgia College of Veterinary Medicine _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: This e-mail message, from Children's Hospital, Columbus, Ohio, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes pursuant to Children's Hospital's confidentiality policies. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From galinadeyneko <@t> yahoo.com Tue Mar 6 09:56:48 2007 From: galinadeyneko <@t> yahoo.com (Galina Deyneko) Date: Tue Mar 6 09:56:56 2007 Subject: [Histonet] (no subject) Message-ID: <20070306155648.21001.qmail@web33103.mail.mud.yahoo.com> Dear Dr.Scouten, Can I place fresh specimens in sucrose fro cryoprotection without preliminary fixation in formalin?. I worked with hearts and vessels from mice, rabbits, monkeys. Thank you. Galina Deyneko Novartis , Cambridge MA. phone: 617-871-7613 --------------------------------- Need Mail bonding? Go to the Yahoo! Mail Q&A for great tips from Yahoo! Answers users. From staceylburton <@t> yahoo.com Tue Mar 6 11:02:04 2007 From: staceylburton <@t> yahoo.com (Stacey Burton) Date: Tue Mar 6 11:02:20 2007 Subject: [Histonet] LIS System search Message-ID: <582627.49871.qm@web30003.mail.mud.yahoo.com> Hello fellow histonetiers~ I am currently looking for a new LIS system. Our lab performs general anatomic pathology ~ cytology and histology. We are looking for a system that will allow us to: `run paperless `bar code cassettes and slides `deliver reports via Internet `able the clinician to pull reports from their account on our system `remote print `billing interface `voice recognize `perform management reports I'm currently looking at AP Easy but we are still open to all systems. If you have any knowledge to "throw into the bucket" for our decision making, it would be wonderful!! Thanks, Stacey Burton, H.T. (ASCP) Precision Pathology Service SA TX ____________________________________________________________________________________ Now that's room service! Choose from over 150,000 hotels in 45,000 destinations on Yahoo! Travel to find your fit. http://farechase.yahoo.com/promo-generic-14795097 From Kristopher.Kalleberg <@t> unilever.com Tue Mar 6 12:22:05 2007 From: Kristopher.Kalleberg <@t> unilever.com (Kalleberg, Kristopher) Date: Tue Mar 6 12:22:13 2007 Subject: [Histonet] paraffin vs. frozen Message-ID: <0E6BC087F70F9C47ACFF2C203D6E329C0208E4C8@NTRSEVS30002.s3.ms.unilever.com> If anyone could give some insight as to what the differences are between frozen sections and paraffin sections when performing IHC I would greatly appreciate it. I am running a study and the results for a few of the markers I am not to happy with and am contemplating changing over to frozen sections for upcoming studies. Is there any rhyme or reason as to why antibodies work better with frozens sections or paraffin sections? Any help will of course be greatly appreciated. Kris Kalleberg Research Scientist Unilever R&D 40 Merritt Blvd. Trumbull, CT 06611 (203) 381-5765 From DDittus787 <@t> aol.com Tue Mar 6 12:29:33 2007 From: DDittus787 <@t> aol.com (DDittus787@aol.com) Date: Tue Mar 6 12:30:06 2007 Subject: [Histonet] paraffin vs. frozen Message-ID: Kris: IHC tends for some markers to work better on frozens due to the lack of crosslinked proteins from fixation. It has been my experience when doing IHC to usually lessen incubation times to eliminate high backgrounds and overstaining, or to use very high titers, but this means you need to do studies and titrations to determine optimal conditions for frozen section IHC before standardizing any protocol. Dana Dittus Biosciences Product Manager Polysciences,Inc.


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AOL now offers free email to everyone. Find out more about what's free from AOL at http://www.aol.com. From PMonfils <@t> Lifespan.org Tue Mar 6 12:19:55 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Tue Mar 6 12:46:30 2007 Subject: [Histonet] (no subject) In-Reply-To: <20070306155648.21001.qmail@web33103.mail.mud.yahoo.com> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273C4D@LSRIEXCH1.lsmaster.lifespan.org> No. Fresh tissue placed into such a hypertonic solution will be damaged. The cells will collapse as water is rapidly extracted from them by osmosis. And even if the osmotic tension of the solution itself didn't cause physical damage, leaving tissue unfixed for 24 hours or more will allow undesireable chemical changes to occur. From Sheri.Meilus <@t> va.gov Tue Mar 6 12:52:58 2007 From: Sheri.Meilus <@t> va.gov (Meilus, Sheri D.) Date: Tue Mar 6 12:53:25 2007 Subject: [Histonet] FW: question about online BS in histotechnology Message-ID: Dear Histonetters, Here is an excerpt from an email sent by Rhoda Jost at FCCJ describing where they're at in considering implementing an online BS in Histotechnology: "We are in the exploration stage at this time. These are our constraints: 25% of the degree credits must come from FCCJ (at least 30 credits) 4 year degree = 120 credits Roughly 1/2 must be 3000 and 4000 level courses We will need to have a bridge program for our current AS-HT degree. We plan an on-line degree with possible research projects carried out in student's job sites for some of the HT classes. We are thinking of some management courses as part of the curriculum. That is as far as we have gotten---I welcome suggestions from those in the field of histotechnology. Let me also stress that this is very tentative." I personally would like to thank you all for all your letters of interest. I've forwarded them to her and Dr. Henning at FCCJ. I'm still receiving them and I'll continue to forward them. She states that they welcome suggestions from those of us in the field, so I continue to encourage you to be in contact with both her and Dr. Henning. It's looking very encouraging so let's continue to be in contact with them!! I've had very positive conversations with Pathologists where I work, as well as others in the community who've offered to help, if appropriate, with the research projects which she mentioned to be carried out at the students' job sites, so that level of communication from Pathologists to them would also be very helpful in my opinion. I'll continue to update the group on where things are with FCCJ, but again, the lines of communication are wide open for you all to contact them directly. Sheri From lidiaz <@t> ucsd.edu Tue Mar 6 12:57:49 2007 From: lidiaz <@t> ucsd.edu (Diaz, Liliana) Date: Tue Mar 6 12:59:28 2007 Subject: [Histonet] Pathos question. Message-ID: <550F625770202C4CBE4EF3296F0805A9062A6AAA@ucsdhc-exch4.AD.UCSD.EDU> Hello Histonetters. I'm a current Histotech at UC San Diego and would like to know if anyone working with the Pathos (Milestone) processor is willing to share their protocol. Our lab will mainly be using it for biopsies. Thanks, Lily Diaz 200 W Arbor Dr. San Diego, Ca. 92103 619-543-6167 From katri <@t> cogeco.ca Tue Mar 6 13:08:07 2007 From: katri <@t> cogeco.ca (Katri Tuomala) Date: Tue Mar 6 13:08:13 2007 Subject: [Histonet] paraffin vs. frozen References: <0E6BC087F70F9C47ACFF2C203D6E329C0208E4C8@NTRSEVS30002.s3.ms.unilever.com> Message-ID: <003a01c76022$c69756f0$6a9a9618@Katri> Kris, from clinical laboratory point of view, most of the time we don't have a choice. Most of our work is done retrospectively on formalin fixed paraffin embedded material (FFPE). This is why many manufacturers produce antibodies meant for this purpose. In selection of the antibodies, the type of material you have to work with has to be kept in mind. But the fact is, that not all antigens can survive or they are masked by the FFPE process. The masking has been largely overcome by many types of retrieval procedures : enzymatic digestion and use of heat in different buffers. But the antigens, which don't survive FFPE, must be demonstrated in frozen sections, fixed in various fixatives (or not fixed at all)depending on the antigen to be demonstrated. Usually these procedures are much faster, but morphology often suffers. In a clinical lab, a procedure has to be in place for collecting specimens for frozen section work. For instance a technologist has to attend, when renal or muscle biopsies are performed by a clinician and proper and prompt handling of the specimen is essential. I hope this is of some help to you. I'm sure other histonet members will come forward for more information. Katri Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "Kalleberg, Kristopher" To: Sent: Tuesday, March 06, 2007 1:22 PM Subject: [Histonet] paraffin vs. frozen If anyone could give some insight as to what the differences are between frozen sections and paraffin sections when performing IHC I would greatly appreciate it. I am running a study and the results for a few of the markers I am not to happy with and am contemplating changing over to frozen sections for upcoming studies. Is there any rhyme or reason as to why antibodies work better with frozens sections or paraffin sections? Any help will of course be greatly appreciated. Kris Kalleberg Research Scientist Unilever R&D 40 Merritt Blvd. Trumbull, CT 06611 (203) 381-5765 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Tue Mar 6 13:37:15 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Mar 6 13:37:16 2007 Subject: [Histonet] block-orientation Message-ID: <000701c76026$deaf1b70$6412a8c0@dielangs.at> A quite simple question: What is the better way of block-orientation in a rotation-microtome for kidney needle biopsies? With the biopsy parallel or normal to the knife? To get a good ribbon without wrinkels in the tissue? Gudrun From LSebree <@t> uwhealth.org Tue Mar 6 13:49:29 2007 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Tue Mar 6 13:49:36 2007 Subject: [Histonet] block-orientation In-Reply-To: <000701c76026$deaf1b70$6412a8c0@dielangs.at> Message-ID: Our renal tech cuts both frozen and paraffin renal biopsies perpendicular to the blade. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Tuesday, March 06, 2007 1:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] block-orientation A quite simple question: What is the better way of block-orientation in a rotation-microtome for kidney needle biopsies? With the biopsy parallel or normal to the knife? To get a good ribbon without wrinkels in the tissue? Gudrun From gcallis <@t> montana.edu Tue Mar 6 14:20:45 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Mar 6 14:20:57 2007 Subject: [Histonet] block-orientation In-Reply-To: <000701c76026$deaf1b70$6412a8c0@dielangs.at> References: <000701c76026$deaf1b70$6412a8c0@dielangs.at> Message-ID: <6.0.0.22.1.20070306131655.01b1b258@gemini.msu.montana.edu> I did a compromise, angled the biopsy in the block so I could gently stretch it on the water bath. We sectioned at 2 um, could do 1 um, and used a harder paraffin with either high profile or low profile blades, Accuedge from Sakura always gave us superb sections. At 12:37 PM 3/6/2007, you wrote: >A quite simple question: > >What is the better way of block-orientation in a rotation-microtome for >kidney needle biopsies? > >With the biopsy parallel or normal to the knife? To get a good ribbon >without wrinkels in the tissue? > > > >Gudrun > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From histobr <@t> yahoo.com Tue Mar 6 14:31:36 2007 From: histobr <@t> yahoo.com (Ruser Rebecca) Date: Tue Mar 6 14:31:43 2007 Subject: [Histonet] TSH District II meeting - March 14 - Houston,TX Message-ID: <966737.3068.qm@web56507.mail.re3.yahoo.com> Open invitation to attend: Vision Biosystems together with Texas Society for Histotechnology is introducing the visionary symposium series. Topic - Advancing the Science of Histology Wednesday, March 14 from 9:00 to 5:00. Registration at 8:30 a.m. Contact Sarah Thorley @ 1-800-753-7264 to RSVP or email at sarah.thorley@vision-bio.com. Keynote speaker is Dr. Michael T. Deavers, M.D. Anderson, Houston, TX Location - Trevisio at the Commons The John T McGovern Texas Med Center Commons Bldg. 6650 Bertner Ave Houston, TX --------------------------------- It's here! Your new message! Get new email alerts with the free Yahoo! Toolbar. From gcallis <@t> montana.edu Tue Mar 6 14:40:01 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Mar 6 14:40:08 2007 Subject: Successful IHC with Re: [Histonet] paraffin vs. frozen In-Reply-To: <0E6BC087F70F9C47ACFF2C203D6E329C0208E4C8@NTRSEVS30002.s3.m s.unilever.com> References: <0E6BC087F70F9C47ACFF2C203D6E329C0208E4C8@NTRSEVS30002.s3.ms.unilever.com> Message-ID: <6.0.0.22.1.20070306132303.01b87ce8@gemini.msu.montana.edu> Kristopher, What species are you working with? And what markers? Basically, it comes down to fixation of the antigen - whether IHC will be successful after crosslinking fixatives followed by antigen recovery (not always needed) or having to use another type of fixative to reduce aldehyde crosslinking (Paraformaldehyde-llysine- periodate (PLP), or avoid cross linking entirely and resort to precipitating fixatives (cold acetone, acetone/absolute ethanol for murine CD markers) or a Zinc TRIS Buffer formalin free fixative developed by Beckstead. Unfortunately, one thing for all doesn't work for some of the touchy antigens. If you are working with murine tissue, and doing CD markers, it is very common to have many antigens (CD markers) totally compromised by any aldehyde fixation with either formalin or paraformaldehyde followed by paraffin processing. The worse scenario is needing several markers on one experimental tissue and two markers will not stain after formalin fixed paraffin embedded tissues (FFPE) even after retrieval or digestion methods. This is the reason we do cryotomy for 99.5% of our murine work. There are going to be times when FFPEwill not work with other species either and you have to use cryotomy. Due to this, we simply gave up using FFPE, and now exclusively do fresh tissue frozen sections. The nice thing about the latter, if one like flourescent work, there is no aldehyde induced autofluorescence to deal with. Good luck Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 At 11:22 AM 3/6/2007, you wrote: >If anyone could give some insight as to what the differences are between >frozen sections and paraffin sections when performing IHC I would >greatly appreciate it. I am running a study and the results for a few >of the markers I am not to happy with and am contemplating changing over >to frozen sections for upcoming studies. Is there any rhyme or reason >as to why antibodies work better with frozens sections or paraffin >sections? Any help will of course be greatly appreciated. > >Kris Kalleberg >Research Scientist >Unilever R&D >40 Merritt Blvd. >Trumbull, CT 06611 >(203) 381-5765 >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Meera.Bansal <@t> chsli.org Wed Mar 7 08:25:29 2007 From: Meera.Bansal <@t> chsli.org (Bansal, Meera) Date: Wed Mar 7 08:25:42 2007 Subject: [Histonet] fungus control for PASfungus Message-ID: Is it acceptable to use kidney tissue as a control for PAS stain when looking for fungus? We have been having a debate on this issue and would like to know what you all think. Meera Bansal MD Mercy Medical Center Rockville Centre, NY The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. From wprichett <@t> ohri.ca Wed Mar 7 08:30:54 2007 From: wprichett <@t> ohri.ca (Prichett, Wendy) Date: Wed Mar 7 08:31:03 2007 Subject: [Histonet] Please unsubscribe Message-ID: <0133EAC2EDC7D74D944267D639E49EF2014D44C5@ohexch04.civic1.ottawahospital.on.ca> -------------------- Confidentiality Statement - The contents of this e-mail, including its attachment, are intended for the exclusive use of the recipient and may contain confidential or privileged information. If you are not the intended recipient, you are strictly prohibited from reading, using, disclosing, copying, or distributing this e-mail or any of its contents. If you received this e-mail in error, please notify the sender by reply e-mail immediately or the Privacy Office (privacy@ottawahospital.on.ca ) and permanently delete this e-mail and its attachments, along with any copies thereof. Thank you. Avis de confidentialité – Ce courriel, y compris ses pièces jointes, s’adresse au destinataire uniquement et pourrait contenir des renseignements confidentiels. Si vous n’êtes pas le bon destinataire, il est strictement interdit de lire, d’utiliser, de divulguer, de copier ou de diffuser ce courriel ou son contenu, en partie ou en entier. 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Merci. -------------------- From JWEEMS <@t> sjha.org Wed Mar 7 08:33:56 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Mar 7 08:34:31 2007 Subject: [Histonet] fungus control for PASfungus In-Reply-To: Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D36BB@sjhaexc02.sjha.org> That will prove your stain worked, but I would use the same control you use for GMS - that way you know how the the fungus looks... my 2 cents! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Bansal, Meera Sent: Wednesday, March 07, 2007 9:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fungus control for PASfungus Is it acceptable to use kidney tissue as a control for PAS stain when looking for fungus? We have been having a debate on this issue and would like to know what you all think. Meera Bansal MD Mercy Medical Center Rockville Centre, NY The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From Howard.Gray <@t> dako.com Wed Mar 7 08:47:14 2007 From: Howard.Gray <@t> dako.com (Howard Gray) Date: Wed Mar 7 08:47:31 2007 Subject: [Histonet] please unsubscribe Message-ID: Unsubscribe please Howard From eearle <@t> abrazohealth.com Wed Mar 7 08:58:03 2007 From: eearle <@t> abrazohealth.com (Earle, Elizabeth) Date: Wed Mar 7 08:58:14 2007 Subject: [Histonet] goldfish processing Message-ID: <3961D92A950C8F4CAB37AAC02F21FE30AB112F@mail-srv02.vhswest.local> I have some goldfish which have been placed intact into formalin. They were exposed to different contaminants in water before being sacrificed. What is the best way to demonstrate the gills? Should I do cross sections or sagital sections? Thanks for any help; if someone can point me to a reference that would be great too. PS this is for a science fair project, favor for a friend. Thanks EE From cbobrowi <@t> mcw.edu Wed Mar 7 09:34:52 2007 From: cbobrowi <@t> mcw.edu (Bobrowitz, Carol) Date: Wed Mar 7 09:35:01 2007 Subject: [Histonet] Permanent Mounting Medium? Message-ID: <8F78639AC56F4143B267FE5F5A1B92C8F0ADE5@guyton.phys.mcw.edu> Hi, We've been using DAKO's Fast Drying Permanent Mounting Medium (S3026-30). We have been very satisfied. DAKO no longer sell this item. Does anyone know if this item is marketed under a different name and Vendor? We manually coverslip and liked the fast drying aspect instead of the slow hardening of Permount. We also liked it because it dried clearer then Permount. All suggestions will be appreciated. Thank you all in advance. Carol Ann Bobrowitz Medical College of Wisconsin Physiology, Histology cbobrowi@mcw.edu From gcallis <@t> montana.edu Wed Mar 7 10:03:23 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed Mar 7 10:03:41 2007 Subject: [Histonet] fungus control for PASfungus In-Reply-To: References: Message-ID: <6.0.0.22.1.20070307085840.01b5d640@gemini.msu.montana.edu> Even in research, our positive control always contains fungus. For kidney tissue, especially renal biopsies, we do the PAS differently than for fungus anyway, plus the sections were always cut at 2 um in order to see the thin basement membrane staining. We do not use Periodic acid as the oxidizer for fungus staining, we prefer using chromic acid, much stronger in order to get better positive staining of a fungus. As an aside, Frieda Carson and Jerry Fredenburgh published in Journal of Histotechnology, the pitfalls of getting false negatives with PAS for fungus staining. a positive control for PAS should contain fungus. At 07:25 AM 3/7/2007, you wrote: >Is it acceptable to use kidney tissue as a control for PAS stain when >looking for fungus? We have been having a debate on this issue and would >like to know what you all think. >Meera Bansal MD >Mercy Medical Center >Rockville Centre, NY Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From Tiffany.Price <@t> thomaswv.org Wed Mar 7 10:08:29 2007 From: Tiffany.Price <@t> thomaswv.org (Price, Tiffany) Date: Wed Mar 7 10:08:53 2007 Subject: [Histonet] procedure for disposal of DAB waste from Ventana Stainer Message-ID: <7B5F5D9A00739F43A1819403EC7CF49103C3219B@thm-mail.thomaswv.org> Does anyone have a procedure for disposal of DAB waste? I have a procedure that uses magnesium permanganate, but I understand that you can also use bleach. Thanks for any information- Tiffany Confidentiality Notice: This email message including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privilege information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy ALL copies of the message. From raestask <@t> grics.net Wed Mar 7 10:14:29 2007 From: raestask <@t> grics.net (Rae Staskiewiez) Date: Wed Mar 7 10:15:40 2007 Subject: [Histonet] Illinois Society for Histotechnologists Spring Meeting Message-ID: <000601c760d3$b001c360$0302a8c0@rae1ktsbyhej72> The Illinois Society for Histotechnologists will hold their Spring Symposium on May 17-18, 2007 at The Chateau in Bloomington, IL. Following is the program. If you have any questions, or for further information, contact Jane Chladny at mchladny@uiuc.edu or call 217-333-8708. 7:00-8:00 Registration Rolls and coffee provided 8:00-8:15 President's Welcome Maureen Doran 8:15-9:15 HPV Dr. Amy Budke 9:15-10:00 Break/Exhibits 10:00-11:00 The Histological Role of Targeted Therapy Testing in Cancer Ray Ortiz 11:00-12:00 Learn Different Mohs Techniques Lisa Jackson 12:00-1:00 Lunch Thursday, May 17 Afternoon Workshops 1:00-4:30 pm (break from 2:30-3:00 with vendors) #1 Colors Dr. Robin Orr #2 Strategies for Success Ada Feldman/ in Immunohistochemistry Dee Wolfe #3 Ready or Not Here It Comes: Donna Willis Microwave Technology Don't miss the Wine and Cheese Reception with Vendor Auction!!! 5-7 pm Thursday, in the exhibit room Friday, May 18 Registration 7:00-8:00 am Morning Workshops 8:00-11:45 am (break from 9:45-10:30 with vendors) #4 DIY Basic Repair and Maintenance Matt Mincer/ of Equipment Stan Weglarz #5 Basic Review of the H&E Stain Mary Raye Hestand #6 How Do I Find It, and What Do I Do With It When I Do Find It? Charlie Dorner Lunch/Awards 11:45-1:00 Afternoon Workshops 1:00-4:30 (break from 2:30-3:00) #7 Stars of the Silver Screen Joan Vesey #8 The SWEET Workshop: Smart Working Environment Ergonomics Training Jan Minshew Jan Minshew Reservations may be made at: The Chateau Hotel and Conference Center 1601 Jumer Drive, Bloomington, IL 61704 Rooms $89.00 plus 12% tax per night Call 309-662-2020 Please reserve your room by April 16th, and mention ISH when making your reservation to receive the symposium rate. The hotel offers an indoor pool, sauna, whirlpool, & fitness room. An outdoor mall is within walking distance. Check out www.chateauhotel.biz for more information. From mcauliff <@t> umdnj.edu Wed Mar 7 10:38:43 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Mar 7 10:38:34 2007 Subject: [Histonet] Permanent Mounting Medium? In-Reply-To: <8F78639AC56F4143B267FE5F5A1B92C8F0ADE5@guyton.phys.mcw.edu> References: <8F78639AC56F4143B267FE5F5A1B92C8F0ADE5@guyton.phys.mcw.edu> Message-ID: <45EEEA93.6070609@umdnj.edu> I use DPX, much better than Permount. I don't know if it is the same as the DAKO product. Geoff Bobrowitz, Carol wrote: >Hi, > > > >We've been using DAKO's Fast Drying Permanent Mounting Medium >(S3026-30). > >We have been very satisfied. > >DAKO no longer sell this item. > >Does anyone know if this item is marketed under a different name and >Vendor? > > > >We manually coverslip and liked the fast drying aspect instead of the >slow hardening > >of Permount. We also liked it because it dried clearer then Permount. > > > >All suggestions will be appreciated. Thank you all in advance. > > > >Carol Ann Bobrowitz > >Medical College of Wisconsin > >Physiology, Histology > >cbobrowi@mcw.edu > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From PMonfils <@t> Lifespan.org Wed Mar 7 11:22:13 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Mar 7 11:22:23 2007 Subject: [Histonet] goldfish processing In-Reply-To: <3961D92A950C8F4CAB37AAC02F21FE30AB112F@mail-srv02.vhswest.local> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273C4E@LSRIEXCH1.lsmaster.lifespan.org> Given the information you provided, I would say cut the sections in whatever plane will provide the greatest surface area to view (presumably sagital). While I don't have much experience sectioning fish gills, I assume they will be rather curved when excised. You might want to hold them flat during processing, so it will be easier to get a complete section. For this purpose I use cassette lids from which I have removed the outer rim. This gives me a flat, rigid, solvent-resistant "screen" that just fits inside a cassette. When I have a curved, flexible tissue that I want to process flat, I place the tissue in the cassette, drop one of these modified lids on top of it (or even two of them if the tissue is very thin), then close the cassette lid as usual. The tissue is pressed lightly between the "inner lid" and the cassette bottom, thereby remaining flat during processing. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Earle, Elizabeth > Sent: Wednesday, March 7, 2007 6:58 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] goldfish processing > > I have some goldfish which have been placed intact into formalin. They > were exposed to different contaminants in water before being sacrificed. > What is the best way to demonstrate the gills? Should I do cross > sections or sagital sections? Thanks for any help; if someone can point > me to a reference that would be great too. > > PS this is for a science fair project, favor for a friend. > > Thanks > > EE > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From FUNKM <@t> mercyhealth.com Wed Mar 7 11:24:53 2007 From: FUNKM <@t> mercyhealth.com (Marcia Funk) Date: Wed Mar 7 11:25:27 2007 Subject: [Histonet] blocks and workload Message-ID: <45EEA10502000016003F0732@nodcmsngwia1.trinity-health.org> Hello, Need your help with workload and blocks per tech. Is there a standard for cutting somewhere ? At times we have 300 blocks of skin and general type specimens plus the specials that are 60 to 100 additional. Most of the time we have just two cutting, one on specials and one on frozen. Short staff and lab management thinks life is good in Histology. Marcia From FUNKM <@t> mercyhealth.com Wed Mar 7 11:52:20 2007 From: FUNKM <@t> mercyhealth.com (Marcia Funk) Date: Wed Mar 7 11:52:54 2007 Subject: [Histonet] Microwave tissue processor Message-ID: <45EEA77402000016003F0797@nodcmsngwia1.trinity-health.org> Hi Please let me know your thoughts on the Sakura auto microwave processor stainer ? thanks mrf From GMartin <@t> marshallhospital.org Wed Mar 7 12:08:55 2007 From: GMartin <@t> marshallhospital.org (GMartin@marshallhospital.org) Date: Wed Mar 7 12:06:24 2007 Subject: [Histonet] Decontamination Message-ID: I would like to know a method for decontaminating a cryostat without shutting the system down. We have just one cryostat! Thank you from California From joseph.j.kang <@t> pfizer.com Wed Mar 7 13:09:20 2007 From: joseph.j.kang <@t> pfizer.com (Kang, Joseph J) Date: Wed Mar 7 13:09:45 2007 Subject: [Histonet] IHC bone tissue adherence issues Message-ID: Dear Histoneters, I have a great favor to ask. I need to find out what procedures I can use to keep bone sections intact on glass slides during IHC staining. There seems to be a lot of tissue lifting or loss during antigen retrieval. I am currently using plus slides on regular paraffin sections and leaving them in the oven for up to 24 hours with no success. I also attempted using the Instrumedics tape transfer system, which seems to retain more tissue than regular sections, but with similar results. We also attempted doing IHC on frozen knee joints using the tape transfer system to skip the antigen retrieval steps without success. Any positive feedback on the matter is greatly appreciated. Joe From gcallis <@t> montana.edu Wed Mar 7 14:07:00 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed Mar 7 14:07:24 2007 Subject: [Histonet] IHC bone tissue adherence issues In-Reply-To: References: Message-ID: <6.0.0.22.1.20070307125045.01b477b8@gemini.msu.montana.edu> Joseph, This was discussed recently on Histonet so check out the Archives too. You did not say if you are using heat retrieval or enzyme digestion? Some people prefer the gentler temperatures of digestion - I recall people using that instead of heat retrieval. With Cryojane tape transfer, you can improve bone adherence to polymer slide by flashing the UV light twice, and I have done it three times. Be careful to let the light source capacitor build up charge, so wait a bit between flashes. Also, be sure your tungsten carbide d profile is absolutely the sharpest it can be in order to obtain very flat sections - it is important to have the section in total contact with the tape at initial rolling and a slide at transfer. You can go from 1/2x to 1X, while 4X polymer slides are super gooey but may be necessary to hold the section down. We like the 1/2 X, but do have the others in case we run into major problems. It may help to NOT dry the bone sections in a hot oven, but to lay sections on slides FLAT on a 37C - 40C slide warmer or on a slide tray in a 37C oven, overnight or longer. Hot temperatures tend to dry out the bone excessively, in particular the cartilage. Overdried bone sections often release (have had it happen too many times) even during routine staining. Whenever possible, we dry at the lower temperature for several days. It does test our patience. Hope this helps At 12:09 PM 3/7/2007, you wrote: >Dear Histoneters, >I have a great favor to ask. >I need to find out what procedures I can use to keep bone sections >intact on glass slides during IHC staining. >There seems to be a lot of tissue lifting or loss during antigen >retrieval. I am currently using plus slides on regular paraffin sections >and leaving them in the oven for up to 24 hours with no success. I also >attempted using the Instrumedics tape transfer system, which seems to >retain more tissue than regular sections, but with similar results. >We also attempted doing IHC on frozen knee joints using the tape >transfer system to skip the antigen retrieval steps without success. >Any positive feedback on the matter is greatly appreciated. > >Joe Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From relia1 <@t> earthlink.net Wed Mar 7 14:20:41 2007 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Mar 7 14:20:52 2007 Subject: [Histonet] Trade in your mittens and scarves for flip flops and a suntan!! Message-ID: Hi Histonetters Trade in your mittens and scarves for flip flops and a suntan!! Why not get your Florida license and move to the sunshine state. Today it is sunny and 80 degrees. In our experience, Florida licensing takes about 6 weeks to process if your application is filled out properly. I will be happy to help you with that. Here are some of the warm sunny locations that have histology positions open. Histology Manager ? Central Florida ? great area for golfers, roller coaster junkies and sun worshippers. Histotech/PA - West Central Florida ? known for it?s boating, fishing, beautiful beaches and amazing sunsets. Histotech ? Northern Central Florida ? beautiful area with camping, fishing horseback riding, hiking and more. Histotech ? Southwestern Florida ? go sailing, deep sea fishing or spend the day at the beach. If you or anyone you know might be interested in hearing more about any of these jobs I can be reached at relia1@earthlink.net or Toll Free at 866-60RELIA (866-607-3542. If you are interested in opportunities in other areas of the country please let me know. I have clients nationwide and am happy to conduct a job search for you FREE of charge. Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net From dcrippen <@t> buckinstitute.org Wed Mar 7 14:46:58 2007 From: dcrippen <@t> buckinstitute.org (Danielle Crippen) Date: Wed Mar 7 14:47:09 2007 Subject: [Histonet] IHC bone tissue adherence issues In-Reply-To: Message-ID: Hi Joe, We have had great success doing the following with mouse bones... Leave bone samples immersed in PFA for at ~7 days...then proceed DECALCIFICATION - Decalcify in Immunocal (Decal Chem), with agitation, changing solution and checking for endpoint daily. o To Check for endpoint mix 4 parts spent decal solution (formic acid) to 1 part 5% ammonium oxalate (w/v)...if there's a white calcium oxalate ppt...another day of decalcification is needed o Once decalcification is complete, run bones under running tap water for a few minutes, then wash in tap water for 1-4 hours o Bones can be stored in PBS until processing can begin PROCESSING - 70% EtOH 1.5 hours - 80% EtOH 1.5 hours - 95% EtOH 2x 1.5 hours - 100% EtOH 2x 1.5 hours - Clearite 3 2x1.5 hours - Tissue Prep 2 paraffin 4x1.5 hours 60 degrees under vacuum - Embed in Tissue Prep 2 Then we section 7um sections onto plus slides (we use Starfrost plus from Mercedes Medical cat# MER7255-90-WH). Let them airdry o/n at RT. Next day bake them lying flat at 60degrees C for 1 hour before IHC. We use a standard IHC protocol only we replace our antigen retrieval protocol with Decal Retrieval soln (Biogenex HK089-5K) which doesn't require heating. Our technique doesn't seem greatly different from anything you've tried...but our bone tissues always stay in tact throughout staining...we've never had a problem... We've only used mouse femur and tibia..so there may be a difference b/t our samples..different bone, different animal??? Hope this is helpful... d Danielle Crippen Morphology and Imaging Core Manager Buck Institute for Age Research 8001 Redwood Blvd. Novato, CA 94945 415-209-2046 dcrippen@buckinstitute.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Kang, Joseph J Sent: Wednesday, March 07, 2007 11:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC bone tissue adherence issues Dear Histoneters, I have a great favor to ask. I need to find out what procedures I can use to keep bone sections intact on glass slides during IHC staining. There seems to be a lot of tissue lifting or loss during antigen retrieval. I am currently using plus slides on regular paraffin sections and leaving them in the oven for up to 24 hours with no success. I also attempted using the Instrumedics tape transfer system, which seems to retain more tissue than regular sections, but with similar results. We also attempted doing IHC on frozen knee joints using the tape transfer system to skip the antigen retrieval steps without success. Any positive feedback on the matter is greatly appreciated. Joe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Wed Mar 7 16:32:43 2007 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Mar 7 16:32:59 2007 Subject: [Histonet] fungus control for PASfungus Message-ID: Acceptable but not optimal. Fungus tends to stain differently to basement membranes with some variability depending on the fungus type and its age (dead fungus tends to stain poorly - need a Chromic acid - Methenamine Silver or a Chromic acid - Schiffs reaction to better show them). It is easy to make your own Fungi control. Fix and process some slices of mouldy bread or fruit. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 > Bandaged Bear Day - Friday 30th March 2007 > For more information and to order merchandise visit www.bandagedbearday.com.au > > -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bansal, Meera Sent: Thursday, 8 March 2007 1:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fungus control for PASfungus Is it acceptable to use kidney tissue as a control for PAS stain when looking for fungus? We have been having a debate on this issue and would like to know what you all think. Meera Bansal MD Mercy Medical Center Rockville Centre, NY The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From dellav <@t> musc.edu Wed Mar 7 17:34:09 2007 From: dellav <@t> musc.edu (Vinnie Della Speranza) Date: Wed Mar 7 17:34:19 2007 Subject: [Histonet] Orlando Regional Medical Center Message-ID: Greetings, if anyone can provide contact information for the histology supervisor and/or Lab Administrator at Orlando Regional Medical Center in Orlando, Florida, I would be grateful if you would contact me off list with this information. thanks Vinnie DellaSperanza Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, SC 29425 Ph: 843-792-6353 fax: 843-792-8974 From leba <@t> hawaii.edu Wed Mar 7 19:38:49 2007 From: leba <@t> hawaii.edu (Heather A Leba) Date: Wed Mar 7 19:39:03 2007 Subject: [Histonet] ovarian tissue coming off slides Message-ID: After a month of trying to troubleshoot this issue can anyone offer some suggestions? I'm mounting fish ovarian tissue and ooctyes and have repeadedly had my sections fall off during the staining process (H & E) during the second xylene step and also during the ETOH. I've tried using tissue from different specimens, replacing my solutions during the dehydration process, changing the staining solutions, using poly-L-lysine coated slides, putting them on a slide warmer, letting them air dry overnight, and now tried charged slides (but have not stained these yet). I'm not sure what else to try and am at my wits end. Please help! Frustrated! Heather Leba UH Manoa, Zoology Dept. From c.m.vanderloos <@t> amc.uva.nl Thu Mar 8 03:13:09 2007 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Thu Mar 8 03:13:40 2007 Subject: [Histonet] RE: paraffin vs. frozen Message-ID: <11f6e0911fbbec.11fbbec11f6e09@amc.uva.nl> Kris, Apart from the remarks already made I would like to add the following: FFPE slides are strictly pre-fixed (first fixed as a block, then cut and stained) and cryostat sections from a fresh frozen tissue block are post-fixed (first cut, then fixed and stained). We have seen a great difference between post-fixation and pre-fixation with IHC of small proteins like cytokines. It appeared that the cytokine leaks away in the fixative in the post-fixation situation, whereas pre-fixation works fine. I will send the paper we wrote as attachment to your private mail. This year a better overview on this matter will be published in Biotechnic & Histochem. Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Tue, 6 Mar 2007 13:22:05 -0500 From: "Kalleberg, Kristopher" Subject: [Histonet] paraffin vs. frozen To: If anyone could give some insight as to what the differences are between frozen sections and paraffin sections when performing IHC I would greatly appreciate it. I am running a study and the results for a few of the markers I am not to happy with and am contemplating changing over to frozen sections for upcoming studies. Is there any rhyme or reason as to why antibodies work better with frozens sections or paraffin sections? Any help will of course be greatly appreciated. Kris Kalleberg Research Scientist Unilever R&D 40 Merritt Blvd. Trumbull, CT 06611 From d.a.faichney <@t> stir.ac.uk Thu Mar 8 03:55:03 2007 From: d.a.faichney <@t> stir.ac.uk (Deborah Faichney) Date: Thu Mar 8 03:55:26 2007 Subject: [Histonet] goldfish processing In-Reply-To: <3961D92A950C8F4CAB37AAC02F21FE30AB112F@mail-srv02.vhswest.local> Message-ID: <38E5B431A5228D46A9A99E0206D5788304F729F3@medwin.ad.stir.ac.uk> if you haven't already processed these.......I routinely process fish gills, mainly salmon but have experience of goldfish also. If you open the operculum (the flap, covering the gills, which opens and closes when the fish is alive) and remove the first gill using the point of your scalpel and cutting at the top and bottom to release. The first gill contains the most contaminants and may look awful in section (although it may be interesting in your case!! since that is what they were exposed to) Generally you should still see an effect on the second gill, and it is this one that we would take by releasing in the same manner. The gill is best viewed by embedding flat and applying gentle pressure, with round tipped forceps,onto the filaments to ensure that they are in the same plane. It is not necessary to cut off the gill arch, unless the fish are very large. We block, trim, then surface decalcify for 30 mins to 1 hour before chilling and cutting at five microns. Hope this helps you Debbie Faichney Institute of Aquaculture Stirling University Scotland, UK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Earle, Elizabeth Sent: 07 March 2007 14:58 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] goldfish processing I have some goldfish which have been placed intact into formalin. They were exposed to different contaminants in water before being sacrificed. What is the best way to demonstrate the gills? Should I do cross sections or sagital sections? Thanks for any help; if someone can point me to a reference that would be great too. PS this is for a science fair project, favor for a friend. Thanks EE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- The University of Stirling is a university established in Scotland by charter at Stirling, FK9 4LA. Privileged/Confidential Information may be contained in this message. If you are not the addressee indicated in this message (or responsible for delivery of the message to such person), you may not disclose, copy or deliver this message to anyone and any action taken or omitted to be taken in reliance on it, is prohibited and may be unlawful. In such case, you should destroy this message and kindly notify the sender by reply email. Please advise immediately if you or your employer do not consent to Internet email for messages of this kind. From abright <@t> brightinstruments.com Thu Mar 8 04:27:24 2007 From: abright <@t> brightinstruments.com (Alan Bright) Date: Thu Mar 8 04:25:20 2007 Subject: [Histonet] IHC bone tissue adherence issues Message-ID: Dear Joe, If you can locate Dr John Dodds, who is an expert at frozen sections of bone & has been in the USA for the last 10 years I am sure he will be able to assist you. He has been using a number of our cryostats at various locations without the need of a tape transfer system on undecalcified bone. Hopefully someone seeing this posting may know his location and inform you. Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com Skype User ID: dazzle0 -----Original Message----- From: Kang, Joseph J [mailto:joseph.j.kang@pfizer.com] Sent: 07 March 2007 19:09 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC bone tissue adherence issues Dear Histoneters, I have a great favor to ask. I need to find out what procedures I can use to keep bone sections intact on glass slides during IHC staining. There seems to be a lot of tissue lifting or loss during antigen retrieval. I am currently using plus slides on regular paraffin sections and leaving them in the oven for up to 24 hours with no success. I also attempted using the Instrumedics tape transfer system, which seems to retain more tissue than regular sections, but with similar results. We also attempted doing IHC on frozen knee joints using the tape transfer system to skip the antigen retrieval steps without success. Any positive feedback on the matter is greatly appreciated. Joe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sonyprasad <@t> hotmail.com Thu Mar 8 06:49:45 2007 From: sonyprasad <@t> hotmail.com (sony prasad) Date: Thu Mar 8 06:49:57 2007 Subject: [Histonet] F4/80-PCNA DOUBLE IMMUNOSTAINING Message-ID: Hi, I'm looking to carry out double immunostaining on formalin fixed and paraffin blocked mouse kidney sections. I need to stain for proliferating macrophage therefore need to use F4/80 marker for macrophage and PCNA for proliferating cells on the same sections. At the moment when I carry out single staining, I use trypsin antigen retrieval for F4/80 and citrate antigen retrieval for PCNA. If you have any suggestions around how I could optimise both stainings on the same sections, I would much appreciate it. Regards, Sony. _________________________________________________________________ Tried the new MSN Messenger? It’s cool! Download now. http://messenger.msn.com/Download/Default.aspx?mkt=en-in From hej01 <@t> health.state.ny.us Thu Mar 8 08:21:58 2007 From: hej01 <@t> health.state.ny.us (Helen E Johnson) Date: Thu Mar 8 08:22:17 2007 Subject: [Histonet] shaker Message-ID: Hi Histonetters, I need a recommendation on a shaker-agitator to use while decalcifying specimens. Thanks. Helen Johnson (hej01@health.state.ny.us) From abright <@t> brightinstruments.com Thu Mar 8 08:55:13 2007 From: abright <@t> brightinstruments.com (Alan Bright) Date: Thu Mar 8 08:53:09 2007 Subject: [Histonet] IHC bone tissue adherence issues Message-ID: Dear Joe, If you can locate Dr John Dodds, who is an expert at frozen sections of bone & has been in the USA for the last 10 years I am sure he will be able to assist you. He has been using a number of our cryostats at various locations without the need of a tape transfer system on undecalcified bone. Hopefully someone seeing this posting may know his location and inform you. Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com Skype User ID: dazzle0 -----Original Message----- From: Kang, Joseph J [mailto:joseph.j.kang@pfizer.com] Sent: 07 March 2007 19:09 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC bone tissue adherence issues Dear Histoneters, I have a great favor to ask. I need to find out what procedures I can use to keep bone sections intact on glass slides during IHC staining. There seems to be a lot of tissue lifting or loss during antigen retrieval. I am currently using plus slides on regular paraffin sections and leaving them in the oven for up to 24 hours with no success. I also attempted using the Instrumedics tape transfer system, which seems to retain more tissue than regular sections, but with similar results. We also attempted doing IHC on frozen knee joints using the tape transfer system to skip the antigen retrieval steps without success. Any positive feedback on the matter is greatly appreciated. Joe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Thu Mar 8 09:48:11 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu Mar 8 09:48:19 2007 Subject: [Histonet] shaker In-Reply-To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E273C4F@LSRIEXCH1.lsmaster.lifespan.org> I do all my decalcification in a large glass beaker with a stirrer. To keep the specimens or cassettes from contacting the stir bar I use a device I made from an empty polyethylene chemical jar. I cut off the bottom of the jar, about an inch from the bottom, so I had sort of a shallow bowl. Then I cut large slots around the periphery, extending from near the center to near the edge, but not cutting through the edge. I did the cutting with a scalpel heated in a bunsen burner, working in a hood. A bit tedious but it was a one-time effort, and worth the trouble. I place my large disc-shaped stir bar (Nalgene StarHead 2.25", available from Fisher) in the bottom of a 2,000 ml beaker, place my device (which is almost as large in diameter as the beaker) inverted over the stir bar, and place a weight (a glass coplin jar lid) on top of the lightweight device to hold it in place. Then place the beaker on a magnetic stirrer, fill with decal solution, add the specimens. I run the stirrer at medium high speed, and it creates a continuous current of decal fluid around the specimens. I have found decalcifying this way to be more efficient than simply allowing the specimens to soak in a stagnant solution. From jennifer.harvey <@t> vanderbilt.edu Thu Mar 8 10:41:55 2007 From: jennifer.harvey <@t> vanderbilt.edu (Jennifer Harvey) Date: Thu Mar 8 10:42:07 2007 Subject: [Histonet] Choosing a blocking reagent Message-ID: We have been hotly debating this subject for over a week in our lab. How do you choose the blocking reagent for immunohistochemistry? If you are using a secondary made in a donkey can you just use any hoofed animal's serum to block?? Thanks Jennifer Harvey Center for Stem Cell Biology Department of Cell and Dev Biology Vanderbilt University 831 Light Hall 2215 Garland Ave. Nashville, TN 37232-0225 Phone: 615-322-4378 Fax: 615-343-2173 jennifer.harvey@vanderbilt.edu From liz <@t> premierlab.com Thu Mar 8 11:03:33 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Mar 8 10:54:36 2007 Subject: [Histonet] Choosing a blocking reagent In-Reply-To: Message-ID: <000001c761a3$b49f49d0$0d00a8c0@domain.Premier> Jennifer We use a serum free protein block, many vendors have them we get ours from Dako. It makes things a bit easier for us, we no longer need to worry about which species or detection system we are using the serum free protein block will work on all of them. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Harvey Sent: Thursday, March 08, 2007 9:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Choosing a blocking reagent We have been hotly debating this subject for over a week in our lab. How do you choose the blocking reagent for immunohistochemistry? If you are using a secondary made in a donkey can you just use any hoofed animal's serum to block?? Thanks Jennifer Harvey Center for Stem Cell Biology Department of Cell and Dev Biology Vanderbilt University 831 Light Hall 2215 Garland Ave. Nashville, TN 37232-0225 Phone: 615-322-4378 Fax: 615-343-2173 jennifer.harvey@vanderbilt.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1971 (20070110) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From timothy.macatee <@t> med.nyu.edu Thu Mar 8 11:28:23 2007 From: timothy.macatee <@t> med.nyu.edu (Timothy Macatee) Date: Thu Mar 8 11:30:48 2007 Subject: [Histonet] Research Histology Technician needed In-Reply-To: Message-ID: We have a full time position available in the Research Histology Core Facility in the Department of Pathology at NYU Medical Center. The person will assist with all research histology needs. Their primary responsibilities include basic histology of paraffin embedded tissues (specimen processing, embedding, sectioning), preparation of cryosections, histochemistry as well as tissue microarray preparation and some immunohistochemistry. Competency with standard office software, such as MS-Word and Excel is also desirable. The position requires a BS degree and some laboratory/research experience. Tim Macatee Research Histology Core New York University School of Medicine 550 First Ave. Department of Pathology. Medical Science Building - Room 521 New York, N.Y. 10016 (212) 263-3888 From gcallis <@t> montana.edu Thu Mar 8 11:44:59 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Mar 8 11:45:21 2007 Subject: [Histonet] Choosing a blocking reagent In-Reply-To: References: Message-ID: <6.0.0.22.1.20070308104115.01b55be8@gemini.msu.montana.edu> Jennifer, A blocking agent can be other than a normal serum block and commonly sold by the various vendors but it pays to try these out before using them all the time. We use a normal serum block and always match the normal serum block to the HOST of the secondary. Then I would be using Donkey in your case. I have seen people use swine serum too but we just follow match to the host rule and very few problems with our murine work. At 09:41 AM 3/8/2007, you wrote: >We have been hotly debating this subject for over a week in our lab. > >How do you choose the blocking reagent for immunohistochemistry? > >If you are using a secondary made in a donkey can you just use any hoofed >animal's serum to block?? Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From tim.morken <@t> thermofisher.com Thu Mar 8 12:06:10 2007 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Thu Mar 8 12:05:39 2007 Subject: [Histonet] IHC QC Postion at ThermoFisher Scientific, Lab Vision products Message-ID: The following postion is open at Lab Vision in Fremont, California. We are recruiting nationwide (USA) and a relocation package is available for the right person. QUALITY CONTROL RESEARCH ASSOCIATE II - IHC Summary of Job: Performs quality control tests on antibodies, detection systems, and ancillary products used for immunohistochemistry. Performs IHC and works with Technical Support to test retainers and returned lots of possible nonconforming products. Performs sectioning of paraffin tissue blocks and frozen tissue blocks. May participate in special projects involving development of new products, either reagent or instrumentation. Major Responsibilities: Performs routine IHC testing of raw materials and manufactured antibody products according to established quality control procedures/permanent work instructions. Performs product IHC stability tests. Releases product as directed by QC IHC Lab Manager. Performs experiments to assess product or procedural problems under direction of QC IHC Lab Manager and/or Technical Support Representative. Maintains and manufactures positive control slide product inventory. Prepares ancillary reagent/buffer products for IHC lab. Performs sectioning of paraffin tissue blocks and frozen tissue blocks. Assists in maintenance of tissue control stock. May be involved in special projects including new product development, product optimization, and optimization requested by sales rep/customers, either reagent or instrumentation. Prepares reports on assigned assays, processes, or quality control tests. Complies with GMP, QSRs, and ISO regulations. Assists in maintaining laboratory equipment to assure accuracy and confidence in performance, and reports equipment problems to Calibration Representative. Complies with Lab Vision/NeoMarkers' Quality Policies and Quality Procedures. Able to work closely with other departments in reaching company goals. Education or Equivalence of Experience: BA/BS in Chemistry, Biochemistry, Biology, or Bioscience with 3 or more years relevant experience. Able to perform all specific laboratory procedures as requested by the QC IHC Lab Manager. Ability to meet deadlines and ensure successful product release in a timely manner. Must be able to write clear and understandable documentation. Good word processing and spreadsheet skills. Familiar with antibody technology, especially a good understanding of proteins, enzymes, and the structures and functions of antibodies. Able to work in a team environment. Certification as Histotechnologist (HTL) and Qualification for Immunohistochemistry (QIHC) by American Society for Clinical Pathology (ASCP) is desirable. Supervisory Responsibilities: May oversee work of QC Research Associate I. Reports To: Quality Control Lab Manager - IHC Please send resumes/CV's to me as a reply to this email. Tim Morken Technical Support Manager Lab Vision - Neomarkers ThermoFisher Scientific 47777 Warm Springs Blvd. Fremont, CA 94539 USA tel: 01-510-991-2840 fax: 01 510-991-2826 Tim.Morken@thermofisher.com web: www.labvision.com www.thermofisher.com Tim Morken Technical Support Manager Lab Vision - Neomarkers ThermoFisher Scientific 47777 Warm Springs Blvd. Fremont, CA 94539 USA tel: 01-510-991-2840 fax: 01 510-991-2826 Tim.Morken@thermofisher.com web: www.labvision.com www.thermofisher.com From jqb7 <@t> cdc.gov Thu Mar 8 12:21:42 2007 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Thu Mar 8 12:22:23 2007 Subject: [Histonet] slide labels Message-ID: Hi everyone, I am looking for microscope slide labels (the type that come on sheets) that are in colors other than white. Anyone know of a manufacturer that handles this type of labels? Thanks, Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov From BFicher <@t> chomp.org Thu Mar 8 12:26:49 2007 From: BFicher <@t> chomp.org (Fischer, R. B) Date: Thu Mar 8 12:30:03 2007 Subject: [Histonet] slide labels References: Message-ID: We get ours from Shamrock Specialty Systems,Inc. 34 Davis Dr.,Bellwood Il 60104 800-323-0249 Fax:800-248-1907 e-mail: sales@shamrocklabels.com Hope this helps R. Brian Fischer Histology Lead Tech Community Hospital of the Monterey Peninsula PO Box H.H. Monterey, Ca. 93942 831-625-4791 Fax: 831-625-4793 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thu 3/8/2007 10:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slide labels Hi everyone, I am looking for microscope slide labels (the type that come on sheets) that are in colors other than white. Anyone know of a manufacturer that handles this type of labels? Thanks, Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This is a transmission from Community Hospital of the Monterey Peninsula. This message and any attached documents may be confidential and contain information protected by state and federal medical privacy statutes. They are intended only for the use of the addressee. If you are not the intended recipient, any disclosure, copying, or distribution of this information is strictly prohibited. If you received this transmission in error, please accept our apologies and notify the sender. Thank you. From eileen.lonergan <@t> verizon.net Thu Mar 8 12:35:17 2007 From: eileen.lonergan <@t> verizon.net (eileen.lonergan@verizon.net) Date: Thu Mar 8 12:35:38 2007 Subject: [Histonet] Chatter in GI Biopsies Message-ID: <29411907.3492341173378917349.JavaMail.root@vms171.mailsrvcs.net> Dear All, We have been noticing an increase in chatter for our GI biopsies over the last month or so. We use both microwave (Milestone) and conventional (VIPs) processors. We were using the microwave mainly for small biopsies with very good results until the increase in chatter occured. We switched to the conventional processors and got about the same results. The temps look fine as well as the solutions. Does anyone out there have an idea on this problem. At this point in time, I am open to just about all suggestions. I can be emailed at my work: mailto:elonergan@convergedx. Thanks. Eileen Lonergan HT(ASCP) ConVerge Diagnostic Services Peabody MA 01960 Eileen Lonergan (207) 749-9617 cell (207) 324-6468 home From mhorne <@t> upei.ca Thu Mar 8 12:47:18 2007 From: mhorne <@t> upei.ca (Margaret Horne) Date: Thu Mar 8 12:53:21 2007 Subject: [Histonet] unknown antibody Message-ID: <45F021F4.29759.1291558@localhost> Hi All , we purchased a slide to demonstrate florescence from a company. One of the antibodies is " se1 " but can't find any info on what this is an antibody to. The company has not replied to us either. Does anyone out there know anything about this antibody? Thanks , Margaret Margaret Horne , Histology Teaching Assistant, Dept. of B.SC., Atlantic Veterinary College, U.P.E.I., 550 University Ave., Charlottetown, P.E.I., C1A 4P3 Canada From brett_connolly <@t> merck.com Thu Mar 8 13:52:17 2007 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Thu Mar 8 13:52:48 2007 Subject: [Histonet] unknown antibody In-Reply-To: <45F021F4.29759.1291558@localhost> References: <45F021F4.29759.1291558@localhost> Message-ID: <355C35514FEAC9458F75947F5270974D0176380F@usctmx1103.merck.com> It might be against sinusoidal endothelial cells...check out PubMed for SE-1. Brett M. Connolly, Ph.D. Research Fellow MRL, Imaging Research Merck & Co., Inc. WP-44K PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaret Horne Sent: Thursday, March 08, 2007 1:47 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] unknown antibody Hi All , we purchased a slide to demonstrate florescence from a company. One of the antibodies is " se1 " but can't find any info on what this is an antibody to. The company has not replied to us either. Does anyone out there know anything about this antibody? Thanks , Margaret Margaret Horne , Histology Teaching Assistant, Dept. of B.SC., Atlantic Veterinary College, U.P.E.I., 550 University Ave., Charlottetown, P.E.I., C1A 4P3 Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------------ From PAMMALU <@t> MSN.COM Thu Mar 8 13:58:51 2007 From: PAMMALU <@t> MSN.COM (PAMM BAKKEN) Date: Thu Mar 8 13:59:00 2007 Subject: [Histonet] ammoniacal and diamine silver Message-ID: Can anyone tell me the difference between ammoniacal and diamine silver solutions? The Gordon and Sweets procedure in my Carson book talks about diamine silver in the principle and then lists ammoniacal silver solution in the reagents. Thanks, Pamm From brett_connolly <@t> merck.com Thu Mar 8 14:00:11 2007 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Thu Mar 8 14:00:41 2007 Subject: [Histonet] looking for protocol - Timm's Stain Message-ID: <355C35514FEAC9458F75947F5270974D01763811@usctmx1103.merck.com> Can anyone please point me to a protocol for Timm's stain...looking for zinc in paraffin and/or frozen sections. Thanks, Brett Brett M. Connolly, Ph.D. Research Fellow MRL, Imaging Research Merck & Co., Inc. WP-44K PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com ------------------------------------------------------------------------------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------------ From chases <@t> upstate.edu Thu Mar 8 14:41:03 2007 From: chases <@t> upstate.edu (Sharon Chase) Date: Thu Mar 8 14:41:18 2007 Subject: [Histonet] stain for intercalated discs Message-ID: <45F02E8F0200001900001CE5@gw4.upstate.edu> Hi There, Does anyone have a specific stain for cardiac intercalated discs?My tissue is mouse heart. Thank you in advance. From meligroc <@t> zgi.com Thu Mar 8 15:12:26 2007 From: meligroc <@t> zgi.com (CRME (Criss Meligro)) Date: Thu Mar 8 15:12:56 2007 Subject: [Histonet] Surgipath's SelectTech Hematoxylin 560 Message-ID: <746E68D5CBB205409B12EC32A61EE40102498819@ned.zgi.com> Just want to share some pathologist pleasing information. We had been reluctant to change from our routine method of H&E but after seeing one batch of slides with Surgipath's staining method compared to our old method.... Well we have switched!!!! Great nuclear detail, brighter clearer stain.....Try it, you'll like it!! From vazquezr <@t> ohsu.edu Thu Mar 8 15:27:49 2007 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Thu Mar 8 15:28:29 2007 Subject: [Histonet] Surgipath's SelectTech Hematoxylin 560 Message-ID: Will work good with frozens? >>> "CRME (Criss Meligro)" 3/8/2007 1:12 PM >>> Just want to share some pathologist pleasing information. We had been reluctant to change from our routine method of H&E but after seeing one batch of slides with Surgipath's staining method compared to our old method.... Well we have switched!!!! Great nuclear detail, brighter clearer stain.....Try it, you'll like it!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Thu Mar 8 16:17:08 2007 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Mar 8 16:17:23 2007 Subject: [Histonet] Chatter in GI Biopsies Message-ID: A few thoughts, Check the microtome - not loose?, is the knife angle right? When the blocks are cooling prior to sectioning is the ice too cold? (This occurred in my lab when we were freezing our ice trays in a -60oC freezer) Is the trimming (or facing) too vigorous? Polish the block before sectioning (take a few sections at 4-5um at normal sectioning speed and discard) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 > Bandaged Bear Day - Friday 30th March 2007 > For more information and to order merchandise visit www.bandagedbearday.com.au > > -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of eileen.lonergan@verizon.net Sent: Friday, 9 March 2007 5:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Chatter in GI Biopsies Dear All, We have been noticing an increase in chatter for our GI biopsies over the last month or so. We use both microwave (Milestone) and conventional (VIPs) processors. We were using the microwave mainly for small biopsies with very good results until the increase in chatter occured. We switched to the conventional processors and got about the same results. The temps look fine as well as the solutions. Does anyone out there have an idea on this problem. At this point in time, I am open to just about all suggestions. I can be emailed at my work: mailto:elonergan@convergedx. Thanks. Eileen Lonergan HT(ASCP) ConVerge Diagnostic Services Peabody MA 01960 Eileen Lonergan (207) 749-9617 cell (207) 324-6468 home _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From HornHV <@t> archildrens.org Thu Mar 8 16:27:19 2007 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Mar 8 16:27:48 2007 Subject: [Histonet] Surgipath's SelectTech Hematoxylin 560 In-Reply-To: <746E68D5CBB205409B12EC32A61EE40102498819@ned.zgi.com> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB703E@EMAIL.archildrens.org> We have tried these stains too and love them!! Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of CRME (Criss Meligro) Sent: Thursday, March 08, 2007 3:12 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Surgipath's SelectTech Hematoxylin 560 Just want to share some pathologist pleasing information. We had been reluctant to change from our routine method of H&E but after seeing one batch of slides with Surgipath's staining method compared to our old method.... Well we have switched!!!! Great nuclear detail, brighter clearer stain.....Try it, you'll like it!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From gcallis <@t> montana.edu Thu Mar 8 17:51:59 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Mar 8 17:52:13 2007 Subject: [Histonet] stain for intercalated discs In-Reply-To: <45F02E8F0200001900001CE5@gw4.upstate.edu> References: <45F02E8F0200001900001CE5@gw4.upstate.edu> Message-ID: <6.0.0.22.1.20070308165113.01b52aa0@gemini.msu.montana.edu> We have always been able to identify these with a good, routine H&E stain. At 01:41 PM 3/8/2007, you wrote: >Hi There, > Does anyone have a specific stain for cardiac intercalated discs?My >tissue is mouse heart. > Thank you in advance. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From pruegg <@t> ihctech.net Thu Mar 8 18:09:55 2007 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Thu Mar 8 18:10:00 2007 Subject: [Histonet] stain for intercalated discs In-Reply-To: <6.0.0.22.1.20070308165113.01b52aa0@gemini.msu.montana.edu> Message-ID: <200703090009.l2909nsw065582@pro12.abac.com> What would be a good stains for thrombin, fibrin and/or platelets? Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Thursday, March 08, 2007 4:52 PM To: Sharon Chase; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] stain for intercalated discs We have always been able to identify these with a good, routine H&E stain. At 01:41 PM 3/8/2007, you wrote: >Hi There, > Does anyone have a specific stain for cardiac intercalated discs?My >tissue is mouse heart. > Thank you in advance. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From barbara <@t> ponath.com Thu Mar 8 18:26:29 2007 From: barbara <@t> ponath.com (Barbara Ponath) Date: Thu Mar 8 18:26:35 2007 Subject: [Histonet] Please unsubscribe Message-ID: <0JEM00M0V184W5ON@vms115.mailsrvcs.net> From mari.ann.mailhiot <@t> leica-microsystems.com Thu Mar 8 18:28:20 2007 From: mari.ann.mailhiot <@t> leica-microsystems.com (mari.ann.mailhiot@leica-microsystems.com) Date: Thu Mar 8 18:27:23 2007 Subject: [Histonet] Chatter in GI Biopsies In-Reply-To: <29411907.3492341173378917349.JavaMail.root@vms171.mailsrvcs.net> Message-ID: Eileen Your chatter could come from a few places. Make sure everything is tight on your microtome, especially the specimen head and knife holder. The other issue or issues could be in fixing and processing. Sometimes heat and too many 100% alcohols make biopsies brittle and/or hard and that can cause chatter. It sounds like this just started happening, so maybe start with your microtome and go from there. Hope this helps. Mari Ann Mailhiot BA HT ASCP Application Specialist Leica Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com eileen.lonergan@v erizon.net Sent by: To histonet-bounces@ histonet@lists.utsouthwestern.edu lists.utsouthwest cc ern.edu Subject [Histonet] Chatter in GI Biopsies 03/08/2007 12:35 PM Dear All, We have been noticing an increase in chatter for our GI biopsies over the last month or so. We use both microwave (Milestone) and conventional (VIPs) processors. We were using the microwave mainly for small biopsies with very good results until the increase in chatter occured. We switched to the conventional processors and got about the same results. The temps look fine as well as the solutions. Does anyone out there have an idea on this problem. At this point in time, I am open to just about all suggestions. I can be emailed at my work: mailto:elonergan@convergedx. Thanks. Eileen Lonergan HT(ASCP) ConVerge Diagnostic Services Peabody MA 01960 Eileen Lonergan (207) 749-9617 cell (207) 324-6468 home _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From joseph.j.kang <@t> pfizer.com Thu Mar 8 18:32:06 2007 From: joseph.j.kang <@t> pfizer.com (Kang, Joseph J) Date: Thu Mar 8 18:32:24 2007 Subject: [Histonet] IHC bone tissue adherence issues In-Reply-To: Message-ID: Danielle, Thanks for responding to my email. Before we go further could you tell me what PFA is? We typically do work on both rats and mice knee joints and paws. We typically fix them in formalin for 24 hours for mouse and 48 hours for rats, then we transfer in 70%ETOH for a couple of days before we place them in Immunocal. I will try your processing schedule for the mouse bones, but do you happen to have a rat processing schedule by any chance? In addition, we do a lot of method development for IHC, do you think that the decal retrieval solution could be used for many IHC staining? Thanks for your information. I will try it out as well. Joe -----Original Message----- From: Danielle Crippen [mailto:dcrippen@buckinstitute.org] Sent: Wednesday, March 07, 2007 2:47 PM To: Kang, Joseph J; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC bone tissue adherence issues Hi Joe, We have had great success doing the following with mouse bones... Leave bone samples immersed in PFA for at ~7 days...then proceed DECALCIFICATION - Decalcify in Immunocal (Decal Chem), with agitation, changing solution and checking for endpoint daily. o To Check for endpoint mix 4 parts spent decal solution (formic acid) to 1 part 5% ammonium oxalate (w/v)...if there's a white calcium oxalate ppt...another day of decalcification is needed o Once decalcification is complete, run bones under running tap water for a few minutes, then wash in tap water for 1-4 hours o Bones can be stored in PBS until processing can begin PROCESSING - 70% EtOH 1.5 hours - 80% EtOH 1.5 hours - 95% EtOH 2x 1.5 hours - 100% EtOH 2x 1.5 hours - Clearite 3 2x1.5 hours - Tissue Prep 2 paraffin 4x1.5 hours 60 degrees under vacuum - Embed in Tissue Prep 2 Then we section 7um sections onto plus slides (we use Starfrost plus from Mercedes Medical cat# MER7255-90-WH). Let them airdry o/n at RT. Next day bake them lying flat at 60degrees C for 1 hour before IHC. We use a standard IHC protocol only we replace our antigen retrieval protocol with Decal Retrieval soln (Biogenex HK089-5K) which doesn't require heating. Our technique doesn't seem greatly different from anything you've tried...but our bone tissues always stay in tact throughout staining...we've never had a problem... We've only used mouse femur and tibia..so there may be a difference b/t our samples..different bone, different animal??? Hope this is helpful... d Danielle Crippen Morphology and Imaging Core Manager Buck Institute for Age Research 8001 Redwood Blvd. Novato, CA 94945 415-209-2046 dcrippen@buckinstitute.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Kang, Joseph J Sent: Wednesday, March 07, 2007 11:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC bone tissue adherence issues Dear Histoneters, I have a great favor to ask. I need to find out what procedures I can use to keep bone sections intact on glass slides during IHC staining. There seems to be a lot of tissue lifting or loss during antigen retrieval. I am currently using plus slides on regular paraffin sections and leaving them in the oven for up to 24 hours with no success. I also attempted using the Instrumedics tape transfer system, which seems to retain more tissue than regular sections, but with similar results. We also attempted doing IHC on frozen knee joints using the tape transfer system to skip the antigen retrieval steps without success. Any positive feedback on the matter is greatly appreciated. Joe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From palladineus <@t> yahoo.com Thu Mar 8 19:53:08 2007 From: palladineus <@t> yahoo.com (Randy Khoo) Date: Thu Mar 8 19:53:15 2007 Subject: [Histonet] Mystery Fixative - Free vs Bound Formaldehyde In-Reply-To: <20061110204109.56915.qmail@web61219.mail.yahoo.com> Message-ID: <20070309015308.75921.qmail@web36506.mail.mud.yahoo.com> Hi All, I was looking at past postings for something which has been bothering me but there doesn't seemed to be any archival material on the subject. If I understand correctly, formaldehyde can exists in free and bound form. Can the bound form be released into the free form over a period of time? Has anyone ever used formaldehyde releasers to preserve materials instead of formaldehyde as such? Regards, Randy --- Rene J Buesa wrote: > Glen: > Perhaps she is referring to "Jores's fixative". > There are 2 formulas, one from 1896 and the other > from 1913 > The original one (1896) is as follows: > Water -- 100 mL + 40% formaldehyde--10 mL + spdium > sulfate -- 2g + magnesium sulfate -- 2g + sodium > chloride -- 1 g > Hope this will help you! > Ren? J. > > "Dawson, Glen" > wrote: > All, > > Our AP supervisor is looking for an older fixative > that she used to use to revitalize staining on > tissue that had been sitting in formalin for months. > > > She seems to think it is called "Yori's, Youri's or > Jori's" fixative and they used to make it on-site > back in the day but the recipe has long since been > lost. > > Any Help Would be Greatly Appreciated, > > Glen Dawson > IHC Manager > Milwaukee, WI > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Cheap Talk? Check out Yahoo! Messenger's low > PC-to-Phone call rates. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ____________________________________________________________________________________ Sucker-punch spam with award-winning protection. Try the free Yahoo! Mail Beta. http://advision.webevents.yahoo.com/mailbeta/features_spam.html From conniegrubaugh <@t> hotmail.com Thu Mar 8 20:19:33 2007 From: conniegrubaugh <@t> hotmail.com (connie grubaugh) Date: Thu Mar 8 20:19:45 2007 Subject: [Histonet] FW: NVSH Message-ID: >From: "Connie J. Grubaugh" >To: >Subject: FW: NVSH >Date: Thu, 8 Mar 2007 08:54:16 -0800 > > > >-----Original Message----- >From: Connie J. Grubaugh >Sent: Thursday, March 08, 2007 8:45 AM >To: jackie.a.perkins@quest.com >Cc: Connie J. Grubaugh >Subject: NVSH > >NEVADA SOCIETY OF HISTOTECHNOLOGY >MARCH 31, 2007 >SUNRISE HOSPITAL SKY ROOM AND RENDEZVOUS ROOM > >START AT 9:00 AM > >MICROWAVE TISSUE PROCESSING IS HERE TO STAY > >PRESENTED BY DENISE GERARD HT/HTL(ASCP) > >LEARNING OBJECTIVES >At the end of this workshop, the participant should be able to do the >following: > >1. Understand what a microwave is and how they are produced. >2. Describe how heat is generated by microwaves. >3. Compare microwave heating versus conventional heating to >accelerate tissue processing. >4. Have complete knowledge of the importance of selecting >"laboratory grade" microwave ovens for histology use. >5. List the primary steps for microwave tissue processing. >6. Understand the relationship of solution volume and specimen size >for adequate microwave tissue processing. >7. Know the inert substances such as paraffin was will not absorb >microwaves and rely on other substances to retain" heat for them. >8. Select appropriate reagents and specimen containers for >microwave tissue processing. >9. Keep laboratory safety at the forefront by discussing >ventilation requirements for laboratory microwave ovens. >10. Formulate a test plan to verify microwave protocols for your >histology laboratory. >11. Develop a quality control program for daily maintenance and >fulfillment of CAP regulations for laboratory microwave ovens. >12. Compare a variety of laboratory microwave tissue processors and >begin to make a decision for what may fit their lab's specific needs. > > >The Learning Objectives will be achieved by the PowerPoint >Presentations, interactive discussion amongst the group and some >hands-on demonstration of the Thermo Electron Shandon Tissue Wave >Microwave Tissue Processor. > > >This is the am Workshop > >We will break for lunch which will be furnished by the vendors. > >At this time there will be a short business meeting and once again it is >election time. So if anyone would like to run for office let it now is >the time. I would like to turn this over to someone else and get some >new blood in the society. >Also at this time there are going to be a lot of raffles for some of the >latest Histology books. >The first one will be the histology book written by John Bancroft, >Marilyn Gamble and is selling for $200.00 on Amazon. >The next book will be Frieda Carsons book. >The AFIP manual. >The Sheehan and Hrapchek Histology book. >There will also be gift certificates to of course Starbucks and other >goodies. > >The Afternoon workshop > > THE SWEET WORKSHOP >Smart Working Environment Ergonomics Training > >By Jan Minshew Marketing Manager from Leica > >Lean to create a working environment that is designed around the >principles of ergonomics. This workshop will proved the tools you need >to protect yourself from work-related musculoskeletal disorders, help >you determine high task and suggest appropriated work methods. It's >designed to give you the tools you need to be able to enjoy a pain free >life at and away from work. Now that is Sweet!!!! > > >There will be a $10.00 charge for these workshops which includes >membership to the NVSH for the next two years. > > > _________________________________________________________________ Get a FREE Web site, company branded e-mail and more from Microsoft Office Live! http://clk.atdmt.com/MRT/go/mcrssaub0050001411mrt/direct/01/ From skgoud69 <@t> yahoo.co.in Thu Mar 8 21:20:03 2007 From: skgoud69 <@t> yahoo.co.in (sanjeev kumar) Date: Thu Mar 8 21:20:18 2007 Subject: [Histonet] RE Message-ID: <308694.67342.qm@web7712.mail.in.yahoo.com> Hello Try overnight at 70 degrees. it will work bai --------------------------------- Here?s a new way to find what you're looking for - Yahoo! Answers From springrosycloud <@t> 126.com Fri Mar 9 07:24:30 2007 From: springrosycloud <@t> 126.com (=?gb2312?B?1qO0us+8?=) Date: Fri Mar 9 07:24:55 2007 Subject: [Histonet] cellular infiltration score Message-ID: <45F1600E.000063.05256@bj126app10.126.com> Hello, everyone. I stained the monoclear cells(CD4,CD8,CD20 and CD68) in kidney tissue sections by immunohistochemistry. I want to know how to score the cellular infiltration. If can I set the score as follows: <100/mm3-normal, >200/mm3-mild, >300/mm3-moderate, ...... I am waiting for your kindly reply. Sincerely, Betty Nanjing University School of Medicine, Research institute of Nephrology, Jinling Hospital, Nanjing, China 210002 Tel: 86-025-81765894 From hej01 <@t> health.state.ny.us Fri Mar 9 09:14:44 2007 From: hej01 <@t> health.state.ny.us (Helen E Johnson) Date: Fri Mar 9 09:15:13 2007 Subject: [Histonet] WNV Antibody Message-ID: Hi Histonetters, Does anyone have a good source for West Nile Virus Antibody to be used on formalin-fixed paraffin-embedded mouse tissue when performing IHC? Helen Johnson (hej01@health.state.ny.us) From rcharles <@t> state.pa.us Fri Mar 9 09:31:37 2007 From: rcharles <@t> state.pa.us (Charles, Roger) Date: Fri Mar 9 09:31:56 2007 Subject: [Histonet] WNV Antibody Message-ID: <12E4E17FEF6EBE4BAE95BEB3CDCB57000943BA0A@enhbgpri04.backup> Hello, I use a polyclonal antibody produced by BioReliance for WNV testing in birds, I do not know if it will work in mouse tissue however. You can call them at 1-800-804-3586 ext 2227 and ask. They do produce monoclonals that might work for your application. Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Helen E Johnson Sent: Friday, March 09, 2007 10:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] WNV Antibody Hi Histonetters, Does anyone have a good source for West Nile Virus Antibody to be used on formalin-fixed paraffin-embedded mouse tissue when performing IHC? Helen Johnson (hej01@health.state.ny.us) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Fri Mar 9 09:48:01 2007 From: shive003 <@t> umn.edu (Jan Shivers) Date: Fri Mar 9 09:48:10 2007 Subject: [Histonet] WNV Antibody References: Message-ID: <001d01c76262$51bc9920$a1065486@auxs.umn.edu> Helen, I also use a WNV antibody from BioReliance, but I use their monoclonal (clone 7H2). If you go with this, you'll have to use a mouse on mouse kit for your purposes. Jan Shivers Univ. of Minnesota Veterinary Diagnostic Lab St. Paul, MN ----- Original Message ----- From: "Helen E Johnson" To: Sent: Friday, March 09, 2007 9:14 AM Subject: [Histonet] WNV Antibody > > Hi Histonetters, > Does anyone have a good source for West Nile Virus Antibody to be > used on formalin-fixed paraffin-embedded mouse tissue when performing IHC? > Helen Johnson (hej01@health.state.ny.us) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jhnspam <@t> aol.com Fri Mar 9 10:05:51 2007 From: jhnspam <@t> aol.com (jhnspam@aol.com) Date: Fri Mar 9 10:06:04 2007 Subject: [Histonet] On-Line Certification for Histo Techs Message-ID: <8C9307BF1A522D0-1A90-38B9@WEBMAIL-DC12.sysops.aol.com> Can anyone tell me if there is an on line program available for Histology that will satisfy the Board od Registry requirements to take the HT exam? If so, what would be the prerequistes to participate in this online program? I currently have 2 techs in my lab that want to take the HT exam but they only have OJT training. Thanks, PJ ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From scoop <@t> mail.nih.gov Fri Mar 9 10:19:15 2007 From: scoop <@t> mail.nih.gov (scoop@mail.nih.gov) Date: Fri Mar 9 10:19:26 2007 Subject: [Histonet] does anyone know how to get better immersion fixation of mouse femur? Message-ID: Dear Histonetters, I have been using fixed mouse femur to do mouse bone marrows. I get much better fixation and preservation of bone marrow if I perfuse the mouse than if I immersion fix the femurs. However, I prefer not to perfusion fix the mouse because perfusion fixation prevents me from collecting other tissues unfixed. Is there a way to improve the fixation of the mouse femurs when I immersion fix? I'm guessing that the problem is I'm not getting good enough penetration - I suppose I could poke a few holes in the femurs? Thanks in advance for your help, Sharon From CBark <@t> memorialcare.org Fri Mar 9 12:43:02 2007 From: CBark <@t> memorialcare.org (Christine Bark) Date: Fri Mar 9 12:43:26 2007 Subject: [Histonet] Fixing non-gyn cytologies Message-ID: Hi all, We have been having a problem with our non-GYN PAPs. I've changed the staining procedure to our pathologists liking but they want a better fixation procedure as well. Currently we spin the samples in a centrifuge, remove the excess liquid, cyto-spin the sediment and spray with Surgipath's cytology fixative. What is everyone else doing? Any help would be much appreciated. Thank you, Christine Bark Senior Histotech, Pathology Saddleback Memorial Medical Center 949-452-3548 cbark@memorialcare.org ______________________________________________________________________________ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. Sign up for your free MemorialCare Medical Information and Access Card at http://www.memorialcare.com/apps/AccessCard/AboutCard.cfm From gcallis <@t> montana.edu Fri Mar 9 13:21:33 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Mar 9 13:21:46 2007 Subject: [Histonet] does anyone know how to get better immersion fixation of mouse femur? In-Reply-To: References: Message-ID: <6.0.0.22.1.20070309120042.01b5b658@gemini.msu.montana.edu> Sharon, If you do not need the middle of the femur, or diaphysis, and only interested in the two ends where majority of marrow resides in femur, then cut the bone open in middle to permit internal access for fixative from these open ends. If possible, try to get a minibone saw for this, i.e. Dremel tool or similar tool, so you don't crush the bone. Poking holes in mouse femurs is more likely to destroy the marrow you want to see since the bone shaft is so tiny - just achieving a tiny hole may be difficult, although this is a technic that works for large animal bones - cutting windows or drilling holes away from lesions or areas you need to see. The other alternative it to open both ends of the femur and push the bone marrow out. One lab here does that for mouse bone marrow and is fine IF you do not want to see trabecular bone with the marrow, cortical bone or cartilage. This is more like a bone marrow biopsy. At 09:19 AM 3/9/2007, you wrote: >Dear Histonetters, > >I have been using fixed mouse femur to do mouse bone marrows. I get much >better fixation and preservation of bone marrow if I perfuse the mouse >than if I immersion fix the femurs. However, I prefer not to perfusion >fix the mouse because perfusion fixation prevents me from collecting other >tissues unfixed. Is there a way to improve the fixation of the mouse >femurs when I immersion fix? I'm guessing that the problem is I'm not >getting good enough penetration - I suppose I could poke a few holes in >the femurs? > >Thanks in advance for your help, >Sharon > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From JMacDonald <@t> mtsac.edu Fri Mar 9 14:00:35 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Mar 9 14:00:42 2007 Subject: [Histonet] On-Line Certification for Histo Techs In-Reply-To: <8C9307BF1A522D0-1A90-38B9@WEBMAIL-DC12.sysops.aol.com> Message-ID: Check out Harford Community College at www.harford.cc.md.us Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu jhnspam@aol.com Sent by: histonet-bounces@lists.utsouthwestern.edu 03/09/2007 08:05 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] On-Line Certification for Histo Techs Can anyone tell me if there is an on line program available for Histology that will satisfy the Board od Registry requirements to take the HT exam? If so, what would be the prerequistes to participate in this online program? I currently have 2 techs in my lab that want to take the HT exam but they only have OJT training. Thanks, PJ ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mohana_g2002 <@t> yahoo.com Fri Mar 9 14:03:55 2007 From: mohana_g2002 <@t> yahoo.com (Mohana Gupta) Date: Fri Mar 9 14:03:56 2007 Subject: [Histonet] Mohana has Tagged you! :) Message-ID: [imgsrv.php?uid=21933990] Mohana G, 24 India Mohana G has added you as a friend Is Mohana G your friend? Please respond or Mohana may think you said no :( [1]Click here to unsubscribe from Tagged, P.O. Box 193152 San Francisco, CA 94119-3152 References Visible links 1. http://www.taggedmail.com/no_more.html?unsem=histonet%40lists.utsouthwestern.edu&tId=130065&fid=cbc9ba81f8beb88c Hidden links: 2. http://www.taggedmail.com/welcome.html?conn=1u7shc5m5&ect=3onnzb3&tId=130065&fid=cbc9ba81f8beb88c 3. http://www.taggedmail.com/welcome.html?conn=1u7shc5m5&ect=3onnzb3&tId=130065&fid=cbc9ba81f8beb88c From pmcardle <@t> ebsciences.com Fri Mar 9 14:04:05 2007 From: pmcardle <@t> ebsciences.com (Phil McArdle) Date: Fri Mar 9 14:04:14 2007 Subject: [Histonet] Microwave-assisted decalcification Message-ID: <45F1BDB5.3010303@ebsciences.com> Hi: This is a question from a vendor: I'd appreciate any input anyone can provide regarding experiences with microwave-accelerated decalcification; successes, failures, favorite reagents and protocols, typical time savings realized, etc. I want to stress that this is not an attempt to co-opt anyone's work or ideas; it's a simple case of always trying to "advance the art." Best regards, Phil McArdle -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Mobile: 860.597.6796 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it, and it was probably my mistake anyway. So please do the right thing and make this e-mail go away. Thank you. From mcauliff <@t> umdnj.edu Fri Mar 9 14:07:29 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Mar 9 14:35:25 2007 Subject: [Histonet] does anyone know how to get better immersion fixation of mouse femur? In-Reply-To: References: Message-ID: <45F1BE81.9090805@umdnj.edu> Remove the other organs first (clamp off the blood supply) then perfuse. Geoff scoop@mail.nih.gov wrote: > Dear Histonetters, > > I have been using fixed mouse femur to do mouse bone marrows. I get > much better fixation and preservation of bone marrow if I perfuse the > mouse than if I immersion fix the femurs. However, I prefer not to > perfusion fix the mouse because perfusion fixation prevents me from > collecting other tissues unfixed. Is there a way to improve the > fixation of the mouse femurs when I immersion fix? I'm guessing that > the problem is I'm not getting good enough penetration - I suppose I > could poke a few holes in the femurs? > > Thanks in advance for your help, > Sharon > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From proctoth <@t> ohsu.edu Fri Mar 9 15:16:24 2007 From: proctoth <@t> ohsu.edu (Thomas Proctor) Date: Fri Mar 9 15:17:07 2007 Subject: Fwd: [Histonet] Mohana has Tagged you! :) Message-ID: Can you please do something about this? Thank you. Thomas From TJJ <@t> Stowers-Institute.org Fri Mar 9 15:39:01 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Fri Mar 9 15:39:32 2007 Subject: [Histonet] Re: does anyone know how to get better immersion... Message-ID: If the joints are of no particular interest to you, then cut off one or both of the long bone ends. That will improve fixation rate for your samples. Best wishes, Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From hlukey <@t> msn.com Fri Mar 9 16:15:06 2007 From: hlukey <@t> msn.com (Hugh Luk) Date: Fri Mar 9 16:15:15 2007 Subject: [Histonet] ovarian tissue coming off slides Message-ID: Heather, I believe your problem is not in your staining procedure, but in ONE of the following: tissue fixation, processing, or microtomy procedures. I used to see problems like you described in tissues from Pacific Island locations that my hospital lab contracted with, where we had no control over the fixatives the tissues were shipped in (Large Igloo-type coolers stuffed with surgical specimens that had collection dates of "Months ago" AND we realized they were using some unbuffered, possibly undiluted formaldehyde solutions). Please tell us more about your entire process. My specific questions to you are: 1) Fixation proceedure; time and what chemical? Is it alcoholic? 2) Tissue processor times for reagents? How many tissue cassettes per run? 3) Temperature of waxes in tissue processor? 4) Temperature of wax in embedding center? Do you keep the cassettes in warm paraffin in the embedding center? If you do, how long before you embed them? 5) While embedding, do your tissue blocks look "Dry" or hard/brittle? 6) Microtomy (sectioning) thickness; 4-6 microns? Do you "Soak" your tissue blocks after trimming them? 7) What type of knife/blades are you using? Does it nicely ribbon off the knife? 8) What slides you are using, and vendor (I have a personal list of "Variable quality" slide vendors). 9) What water do you use for your water bath? pH? Do you add anything to the water? 10) Slide incubator, time AND temperature? Is it a "Forced-Air" dryer? How do the slides look when they are "Dry"? Does water remain? Is the paraffin slightly melted? 11) As for staining: Xylene purity, and is it "Recycled"? 12) Fish ovaries. Decalcification? In my experience; #10 is the most common pitfall. I believe you should be able to provide stainable H&E slides with the tissues you have already processed, with just some "Tricks". I have to admit though, I am a little worried about IHC or In-Situ proceedures. Have you had successful staining? What results you are seeing (clarity of cellular elements)? I would think that your staining solutions are fine unless you have poor staining (as you mention changing your stains). Take-heart in the fact that we, as a group (lab people doing histology), have seen this before and it has frustrated everyone to some degree. Since my work location is only 10-15 minutes away from yours, feel free to call, or ask for advice, especially if you want me to look at something. If you want me to try some microtomy and staining for you, I can do it at my location. Maybe that will help with the trouble-shooting. Hugh Luk, HTL (ASCP) hluk@crch.hawaii.edu or hlkey@msn.com Pathology Shared Resources Lab Manager Cancer Research Center of Hawaii 1236 Lauhala Street, Room 316 Honolulu, HI 96813 Tel: (808) 440-5238 Fax: (808) 586-2982 Cell: (808) 226-4294 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. >From: Heather A Leba >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] ovarian tissue coming off slides >Date: Wed, 07 Mar 2007 15:38:49 -1000 > >After a month of trying to troubleshoot this issue can anyone offer some >suggestions? I'm mounting fish ovarian tissue and ooctyes and have >repeadedly had my sections fall off during the staining process (H & E) >during the second xylene step and also during the ETOH. I've tried using >tissue from different specimens, replacing my solutions during the >dehydration process, changing the staining solutions, using poly-L-lysine >coated slides, putting them on a slide warmer, letting them air dry >overnight, and now tried charged slides (but have not stained these yet). >I'm not sure what else to try and am at my wits end. Please help! > >Frustrated! >Heather Leba > >UH Manoa, Zoology Dept. > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ The average US Credit Score is 675. The cost to see yours: $0 by Experian. http://www.freecreditreport.com/pm/default.aspx?sc=660600&bcd=EMAILFOOTERAVERAGE From Barry.R.Rittman <@t> uth.tmc.edu Fri Mar 9 16:50:43 2007 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Fri Mar 9 16:50:47 2007 Subject: [Histonet] cellular infiltration score References: <45F1600E.000063.05256@bj126app10.126.com> Message-ID: One problem with all counting and image analysis using sections is that cell numbers will vary according to the thickness of the section and the amount of the original volume of tissue contained in that section. As we are comparing sections, we are assuming that sections that have been cut at 5 microns setting are indeed 5 microns. In essence we are comparing the number of cell counted in a volume of tissue. Abercrombie in 1946 showed the fallacy of this assumption and introduced the "Abercrombie Correction Factor". This takes into account the size for example of nuclei. He showed that as thickness of sections increases so does the accuracy of the counts of actual cells. Another problem in histology is the degree of shrinkage especially of soft tissues due to variations in times in solvents, in wax etc. as these may significantly alter the volume of tissue in sections cut at 5 microns even if we assume that they are all the same thickness. One way to determine thickness is to use a substance embedded with the tissue and determine the relative section thickness by the optical density of this material. While I realize that virtually no one will be doing this I feel that we are all somewhat complacent regarding the thickness of the section and that tissue processed at different times or by even slightly different methods are not totally comparable as to volume of tissue contained in sections and therefore numbers of cells, even if these sections were all at exactly the same thickness, a dangerous assumption. While may of us are arrogant enough to feel that we can tell the difference between for example a 5 and a 4 micron section, I am not so sure that our often subjective judgment is appropriate when counting cells is sections. Barry ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of ??? Sent: Fri 3/9/2007 7:24 AM To: histonet Subject: [Histonet] cellular infiltration score Hello, everyone. I stained the monoclear cells(CD4,CD8,CD20 and CD68) in kidney tissue sections by immunohistochemistry. I want to know how to score the cellular infiltration. If can I set the score as follows: <100/mm3-normal, >200/mm3-mild, >300/mm3-moderate, ...... I am waiting for your kindly reply. Sincerely, Betty Nanjing University School of Medicine, Research institute of Nephrology, Jinling Hospital, Nanjing, China 210002 Tel: 86-025-81765894 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From turkekul <@t> gmail.com Sat Mar 10 12:21:52 2007 From: turkekul <@t> gmail.com (mesruh turkekul) Date: Sat Mar 10 12:22:00 2007 Subject: [Histonet] bone fixation Message-ID: Hi Sharon, You can collect the tissues you do not want to fix very quick if possible and then perfuse. If you only immerse fix the femur try to use large volumes of fixative and fix for longer at least 48 hours at 4C by rotating or stirring. Do not use narrow and conical bottom tubes. Use big and wide flat bottom containers. You can add 0.5M EDTA to the fixative as well. Regards, Mesruh Turkekul On 3/9/07, histonet-request@lists.utsouthwestern.edu < histonet-request@lists.utsouthwestern.edu> wrote: > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. does anyone know how to get better immersion fixation of > mouse femur? (scoop@mail.nih.gov) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 9 Mar 2007 11:19:15 -0500 > From: scoop@mail.nih.gov > Subject: [Histonet] does anyone know how to get better immersion > fixation of mouse femur? > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset="us-ascii" ; format="flowed" > > Dear Histonetters, > > I have been using fixed mouse femur to do mouse bone marrows. I get > much better fixation and preservation of bone marrow if I perfuse the > mouse than if I immersion fix the femurs. However, I prefer not to > perfusion fix the mouse because perfusion fixation prevents me from > collecting other tissues unfixed. Is there a way to improve the > fixation of the mouse femurs when I immersion fix? I'm guessing that > the problem is I'm not getting good enough penetration - I suppose I > could poke a few holes in the femurs? > > Thanks in advance for your help, > Sharon > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 40, Issue 12 > **************************************** > From Eric <@t> ategra.com Fri Mar 9 18:06:51 2007 From: Eric <@t> ategra.com (Eric Dye (ext 223)) Date: Sat Mar 10 13:56:58 2007 Subject: [Histonet] Histology opportunities in your area- Can you help? (update3/9/07) Message-ID: Hi - Histonetters - I have both permanent and full time (permanent) J0BS for HistoTechs throughout the US. Are you still working as a HistoTech at ? These permanent and temporary HISTOTECH J0BS are being filled quickly, don't miss out on your dream position - callmetoday ----------------------------------- Temporary HISTOTECH JOBS : ----------------------------------- (Updated Mar 9 2007) 1.South Carolina - Bench - 3 months (starting in August) 2. Florida (East coast) bench- 6 weeks - Need Florida Licence Permanent HISTOTECH J0BS : (Updated Mar 9 2007) ------------------------------------------------------- New Permanent HISTOTECH J0BS Listed below ------------------------------------------------------- 1. Michigan (Ann Arbor Area) Histotech- bench- perm 2. Northern Ohio- Histotech - bench - perm 3. Eastern Pennsylvania - Histotech - Bench - perm 4. Eastern Pennsylvania - Histotech - Bench - perm 5.Florida( Tampa Bay area) Histotech- bench- perm 6.Massachusetts (Greater Boston Area) Histotech- Bench - perm 7.Massachusetts ( Greater Boston Area) Histotech Supervisor 8.Northern Ohio - Histotech - Bench- perm 9. Western Pennsylvania - Bench - perm 10. West Virginia - Bench- perm 11.Massachusetts (Greater Boston Area) Supervisor- perm - HOTHOTHOT!!! 12.Massachusetts (South Boston) - Bench -perm 13. Kentucky (Northwestern)- Bench - perm - 2 openings!! 14. Virginia (Close to North Carolina Border!!) bench- perm 14. Wisconsin - bench - perm ------------------------------------ OTHER HISTOTECH J0BS I have available ---------------------------------- 01. Central Virginia- Histotech- perm 02. Southern California- Histotech- perm 03. Central Florida - Histotech with some MOHS experience - perm - Hot 04. Southeast South Dakota- Histotech - Must have ties to South Dakota- perm- Hot! 05. Hot! Eastern, Massachusetts Seeking Histotechs of all experience levels, Greeaaat paaaay & Great Location - Call Today - 15% Raise Guaranteed! 06.NEWJ0B! Southeast Florida- Histotech - perm - (Need Florida License) 07.Ohio (Southern) - perm - Bench Histotech ( 2 openings) 08.Ohio (Northern) perm- Bench 09.Ohio (Central) perm- Bench 10.Central Florida -perm- Histotech (Need Florida License) 11. Florida (Tampa Bay area) 12. Florida, West Coast - both temp & perm openings- Bench Histotech -Very-Hot 13. New York ( Syracuse area) - Bench Histotech- perm 14. New York City (Long Island) - Bench- perm 15. Las Vegas - Bench Histotech- perm 16. Wisconsin- Histology Supervisor- BrandNew -Hot Hot Hot 17. Central-Illinois-Bench-Dermpath- BrandNew -Hot 18. Northern California- Bench- Routine Histology- BrandNew -------------------------- end list of Histotech Opportunities ------------------------------------- If you are interested in any of the HISTOTECH J0BS listed above - please call me at 800-466-9919 ext 223 or cell - 407-756-5507. Thank you, Eric Dye-Sr Allied Healthcare Recruiter 800-466-9919 ext 223 or Cell - 407-756-5507 P.S.: Feel free to pass along this email and my phone number to anyone who you think might be interested. P.S.S.: The clients are currently interviewing - and the positions will close soon - so if you are interested, please call me today at 1-800-466-9919 ext 229 --------------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing 800-466-9919 ext 223 - voice 407 671 6075 - fax eric@ategra.com To Learn More About Ategra: http://www.ategra.com ---------------------------------------------------------------- If you received this by mistake, or if you wish not to hear from me, please shoot me a mail to let me know and I'll not mail you again. ---------------------------------------------------------------- ---------------------------------------------------------------- Note: this message is intended for: Histonetters at histonet@lists.utsouthwestern.edu ---------------------------------------------------------------- From koellingr <@t> comcast.net Sun Mar 11 10:56:37 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Sun Mar 11 10:56:47 2007 Subject: [Histonet] stain for intercalated discs Message-ID: <031120071556.24248.45F426B50008AE2900005EB822070029539D09020704040A0105@comcast.net> Sharon, I agree with Gayle about a good H&E showing these off nicely with the emphasis on "good" because with washed out, not crisp H&E's, they can become not so easy to see. I don't know for what kind of project you are trying to localize intercalated discs beyond just seeing these on routine H&E's. If it is for a special project here is what I did. In grad school I TA'd in the microanatomy course for all the first year medical students. To help them along a bit in identifying intercalated discs, I made up 125 slides, for their boxed slide sets, of mouse and human heart stained with azocarmine and thionin. The ID's stand out nicely and are easy to find (for sometimes clueless first year med students). Any look at an older histology picture atlas, there are many other stains such special stains to localize these structures. Also, I did an immunoperoxidase on a couple of demo slides (certainly not 125) so they could identify specific components of the ID. You can use antibodies again st cadherin (calcium dependent adherin), desmoplakin, titin (connectin) or many others. Get a good model and description of an intercalated disc. Is just not the wall between 2 adjacent cardiomyocytes. Is an incredibly complex and fascinating structure. But if your goal is just screening and seeing them on routine H&E, that good H&E is certainly easiest and simplest. Ray Koelling Phenopath Labs Seattle, WA -------------- Original message -------------- From: "patsy ruegg" > What would be a good stains for thrombin, fibrin and/or platelets? > Patsy > > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech > 12635 Montview Blvd. #216 > Aurora, CO 80010 > 720-859-4060 > fax 720-859-4110 > pruegg@ihctech.net > www.ihctech.net > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis > Sent: Thursday, March 08, 2007 4:52 PM > To: Sharon Chase; Histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] stain for intercalated discs > > We have always been able to identify these with a good, routine H&E stain. > > At 01:41 PM 3/8/2007, you wrote: > >Hi There, > > Does anyone have a specific stain for cardiac intercalated discs?My > >tissue is mouse heart. > > Thank you in advance. > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > 406 994-6367 > 406 994-4303 (FAX) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sun Mar 11 11:44:20 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Mar 11 11:44:31 2007 Subject: [Histonet] Intercalar heart muscle discs Message-ID: <379986.81249.qm@web61219.mail.yahoo.com> Sharon: The best reccommenden method is that of Dietrich tested by Schmorl in 1928 Sol.A: 1% brilliant black 3B in 0.1% acetic acid Sol.B 1% aq. safranin Sol.C :methanol. 3-4 ?m section from mercury chloride fixed material to solA x 1-5 min → rinse → sol.B x 3-10 min → drain → absolute ethanol til differentiated → sol.C →xylene → coverslip (Peter Gray's 21.16). A good Phophotungstic acid hematoxylin will also do. Ren? J. --------------------------------- Don't get soaked. Take a quick peek at the forecast with theYahoo! Search weather shortcut. From Leslapins <@t> aol.com Sun Mar 11 12:39:28 2007 From: Leslapins <@t> aol.com (Leslapins@aol.com) Date: Sun Mar 11 12:39:39 2007 Subject: [Histonet] Unsubscribe Message-ID: Please remove me from your email list. Thanks... _leslapins@aol.com_ (mailto:leslapins@aol.com)


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AOL now offers free email to everyone. Find out more about what's free from AOL at http://www.aol.com. From koellingr <@t> comcast.net Sun Mar 11 15:25:56 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Sun Mar 11 15:26:06 2007 Subject: [Histonet] Intercalar heart muscle discs Message-ID: <031120072025.1051.45F465D4000777D60000041B22134843739D09020704040A0105@comcast.net> I agree totally with Rene the Brilliant Black is beautiful. I tried it years later on a couple of slides and it was outstanding. It is just at the time of the project, azocarmine and thionin were right on the shelf and not a whole lot of labs have and use Brilliant Black and also I didn't want to fool with mercury fixatives since these future medical doctors (and pathologists) needed to be familiar with FFPE more than with mercury fixation. It all boils down again to what is the purpose of the stain. Ray Koelling Phenopath Labs Seattle, WA -------------- Original message -------------- From: Rene J Buesa > Sharon: > The best reccommenden method is that of Dietrich tested by Schmorl in 1928 > Sol.A: 1% brilliant black 3B in 0.1% acetic acid > Sol.B 1% aq. safranin > Sol.C :methanol. > 3-4 µm section from mercury chloride fixed material to solA x 1-5 min ¨ > rinse ¨ sol.B x 3-10 min ¨ drain ¨ absolute ethanol til > differentiated ¨ sol.C ¨xylene ¨ coverslip > (Peter Gray's 21.16). > A good Phophotungstic acid hematoxylin will also do. > RenEJ. > > > > > --------------------------------- > Don't get soaked. Take a quick peek at the forecast > with theYahoo! Search weather shortcut. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abilger <@t> ptd.net Sun Mar 11 16:29:33 2007 From: abilger <@t> ptd.net (Andrea C. Bilger) Date: Sun Mar 11 15:28:28 2007 Subject: [Histonet] H&E stainer and coverslipper Message-ID: <002a01c76424$5e10ad60$0300a8c0@andrea> Dear All, My lab is looking at purchasing a new H&E stainer and coverlipper this year. I would like to hear which machines any of you prefer and why. No venders please. Thanks in advance. Andrea Bilger From TMcNemar <@t> lmhealth.org Mon Mar 12 06:09:28 2007 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Mon Mar 12 05:09:41 2007 Subject: [Histonet] On-Line Certification for Histo Techs In-Reply-To: <8C9307BF1A522D0-1A90-38B9@WEBMAIL-DC12.sysops.aol.com> Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F467@lmhsmail.lmhealth.org> One in Ohio: http://www.cscc.edu/Histology/cscc.htm Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of jhnspam@aol.com Sent: Friday, March 09, 2007 11:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On-Line Certification for Histo Techs Can anyone tell me if there is an on line program available for Histology that will satisfy the Board od Registry requirements to take the HT exam? If so, what would be the prerequistes to participate in this online program? I currently have 2 techs in my lab that want to take the HT exam but they only have OJT training. Thanks, PJ ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bethcoxx <@t> gmail.com Mon Mar 12 06:50:50 2007 From: bethcoxx <@t> gmail.com (Beth Cox) Date: Mon Mar 12 06:50:58 2007 Subject: [Histonet] Re: Fixing non-gyn cytologies Message-ID: Christine. It sounds like you are cytospinning just the sediment, and the cells aren't seeing fixative until after they've been slammed against the glass slide and been left hanging there (drying) while the cytocentrifuge finishes spinning and brakes and you get the slides out and unclipped and then you spray them.... that's a long time in cell fixation measurements. Basically, you are airdrying them. The rule of thumb is that cytology cells start drying within 2 seconds of contacting the slide. The solution to this is to add Cyotspin Fixative/Saccomanno Fluid/carbowax solution to the sediment before you spin it. Simply put your drop of sediment into the cytocentrifuge funnel, add an equal amount of the Cytospin Fixative on top of it, and spin as usual. The only other thing to keep in mind is that the slides will need to stay in the first 95% alcohol of your staining circuit for a minimum of 10 minutes to soak off the carbowax coating so that it doesn't effect staining. Beth Cox, SCT/HT(ASCP) Schools of Histotechnology Department of Anatomic Pathology, 100RO William Beaumont Hospital 3601 W. 13 Mile Road Royal Oak, MI 48073-6769 Work 248/898-9020 email: bethcox@beaumonthospitals.com Date: Fri, 9 Mar 2007 10:43:02 -0800 From: "Christine Bark" Subject: [Histonet] Fixing non-gyn cytologies To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii Hi all, We have been having a problem with our non-GYN PAPs. I've changed the staining procedure to our pathologists liking but they want a better fixation procedure as well. Currently we spin the samples in a centrifuge, remove the excess liquid, cyto-spin the sediment and spray with Surgipath's cytology fixative. What is everyone else doing? Any help would be much appreciated. Thank you, Christine Bark Senior Histotech, Pathology Saddleback Memorial Medical Center 949-452-3548 cbark@memorialcare.org From sheila_adey <@t> hotmail.com Mon Mar 12 06:51:08 2007 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Mon Mar 12 06:51:19 2007 Subject: [Histonet] Coverslipping IHCs Message-ID: Hi Everyone, We are wondering how other labs coverslip when using AEC. We currently use an aqueous mounting media baked at 80 degrees and then coverslip with Surgipaths micromount. Thanks in advance _________________________________________________________________ Your Space. Your Friends. Your Stories. Share your world with Windows Live Spaces. http://spaces.live.com/?mkt=en-ca From rjbuesa <@t> yahoo.com Mon Mar 12 09:11:56 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 12 09:12:07 2007 Subject: [Histonet] H&E stainer and coverslipper In-Reply-To: <002a01c76424$5e10ad60$0300a8c0@andrea> Message-ID: <123000.62447.qm@web61213.mail.yahoo.com> Andrea: After using them for years (and I mean years) Sakura H&E stainer and film coverslippers are the best. I HAD to work also with instruments from other manufacturers! Ren? J. "Andrea C. Bilger" wrote: Dear All, My lab is looking at purchasing a new H&E stainer and coverlipper this year. I would like to hear which machines any of you prefer and why. No venders please. Thanks in advance. Andrea Bilger _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Finding fabulous fares is fun. Let Yahoo! FareChase search your favorite travel sites to find flight and hotel bargains. From gentras <@t> vetmed.auburn.edu Mon Mar 12 10:03:34 2007 From: gentras <@t> vetmed.auburn.edu (Atoska Gentry) Date: Mon Mar 12 10:03:53 2007 Subject: [Histonet] On-Line Certification for Histo Techs In-Reply-To: References: Message-ID: <45F56BC6.6020409@vetmed.auburn.edu> Hello, please I am very much interested in the availability of online HTL certification, but the link you provided came up as this page can not be displayed. Do you know of another route to take to seek this info? Thanks, Atoska Jennifer MacDonald wrote: > Check out Harford Community College at www.harford.cc.md.us > > > > > Jennifer MacDonald > Director, Histotechnician Training Program > Mt. San Antonio College > 1100 N. Grand Ave. > Walnut, CA 91789 > (909) 594-5611 ext. 4884 > jmacdonald@mtsac.edu > > > > jhnspam@aol.com > Sent by: histonet-bounces@lists.utsouthwestern.edu > 03/09/2007 08:05 AM > > To > histonet@lists.utsouthwestern.edu > cc > > Subject > [Histonet] On-Line Certification for Histo Techs > > > > > > > Can anyone tell me if there is an on line program available for Histology > that will satisfy the Board od Registry requirements to take the HT exam? > If so, what would be the prerequistes to participate in this online > program? I currently have 2 techs in my lab that want to take the HT exam > but they only have OJT training. > > Thanks, > PJ > ________________________________________________________________________ > AOL now offers free email to everyone. Find out more about what's free > from AOL at AOL.com. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Atoska S. Gentry, B.S., HT(ASCP) Research Assistant IV Scott-Ritchey RSCH Center College of Vet. Med Auburn, AL 36849 PH (334) 844-5579 FAX (334) 844-5850 email: gentras@vetmed.auburn.edu From LESCHUKC <@t> trinity-health.org Mon Mar 12 11:19:47 2007 From: LESCHUKC <@t> trinity-health.org (Carmen Leschuk) Date: Mon Mar 12 11:20:23 2007 Subject: [Histonet] Pre-labeling glass slides Message-ID: <45F545630200001500283F50@nodcmsngwia1.trinity-health.org> Recently, I heard of a slide mislabeling that occurred in another histo lab that they determined to be caused by pre-labeling slides before actual use at the microtome. This lab said that if the slides would of been labeled simultaneously (per their policy) as the blocks being cut, the mislabeling would of been prevented. My lab currently pre-labels all of slides before cutting a sequence of 10-20 blocks, which I thought was common practice. My question is, what is common practice? Do other labs have policies forbidding pre-labeling of glass slides? Carmen Leschuk, HT, SLS (ASCP) Supervisor, SJMO-Anatomic Pathology (248)858-6231 From POWELL_SA <@t> Mercer.edu Mon Mar 12 11:19:31 2007 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Mon Mar 12 11:20:50 2007 Subject: [Histonet] GSH meeting Message-ID: <01ME4QB0PZRS8WWLQJ@Macon2.Mercer.edu> Hi Friends, Yes it is me again, posting the program information for our Georgia Society for Histotechnology meeting April 13-15. Great news: I have learned that Callaway Gardens Mountain Creek Inn will still honor the rate ($119 for single, double, triple or quad) they gave us as long as the rooms are available even though the deadline has come and gone. The program in .PDF format can be found on our GSH website www.histosearch.com/gsh. Click on GSH Symposium and the program can be downloaded from there. Also Check out the facilities at Mountain Creek Inn www.callawaygardens.com. Call 1-800 225-5292 for Reservations. Please state you are attending the GSH/ Meeting when making reservations in order to get the discounted rate. Come on down to Georgia to the Georgia Society for Histotechnology meeting. Shirley Powell GSH Secretary From doug <@t> ppspath.com Mon Mar 12 12:40:32 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Mon Mar 12 11:38:19 2007 Subject: [Histonet] Pre-labeling glass slides In-Reply-To: <45F545630200001500283F50@nodcmsngwia1.trinity-health.org> Message-ID: I wouldn't say that the pre-labeling caused it. I would say a lack of attention by the cutting tech caused it. :) It is more time consuming (IMO) to label the slides as you go. What if you are using a slide printer? Would you want to get up and make a slide for every block? There just needs to be steps in place to catch errors before the Pathologist does. Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carmen Leschuk Sent: Monday, March 12, 2007 12:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pre-labeling glass slides Recently, I heard of a slide mislabeling that occurred in another histo lab that they determined to be caused by pre-labeling slides before actual use at the microtome. This lab said that if the slides would of been labeled simultaneously (per their policy) as the blocks being cut, the mislabeling would of been prevented. My lab currently pre-labels all of slides before cutting a sequence of 10-20 blocks, which I thought was common practice. My question is, what is common practice? Do other labs have policies forbidding pre-labeling of glass slides? Carmen Leschuk, HT, SLS (ASCP) Supervisor, SJMO-Anatomic Pathology (248)858-6231 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Mon Mar 12 11:44:09 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Mon Mar 12 11:43:49 2007 Subject: AW: [Histonet] Pre-labeling glass slides In-Reply-To: <45F545630200001500283F50@nodcmsngwia1.trinity-health.org> Message-ID: <000001c764c5$a9088030$6412a8c0@dielangs.at> Wouldn't this be the end of all slideprinters? I can't imagine hopping up before each slide to label it. We also do prelabeling for 10 slides before cutting. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Carmen Leschuk Gesendet: Montag, 12. M?rz 2007 17:20 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Pre-labeling glass slides Recently, I heard of a slide mislabeling that occurred in another histo lab that they determined to be caused by pre-labeling slides before actual use at the microtome. This lab said that if the slides would of been labeled simultaneously (per their policy) as the blocks being cut, the mislabeling would of been prevented. My lab currently pre-labels all of slides before cutting a sequence of 10-20 blocks, which I thought was common practice. My question is, what is common practice? Do other labs have policies forbidding pre-labeling of glass slides? Carmen Leschuk, HT, SLS (ASCP) Supervisor, SJMO-Anatomic Pathology (248)858-6231 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Mar 12 11:44:00 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 12 11:44:08 2007 Subject: [Histonet] Pre-labeling glass slides In-Reply-To: <45F545630200001500283F50@nodcmsngwia1.trinity-health.org> Message-ID: <893017.12787.qm@web61221.mail.yahoo.com> Carmen: Labelling the slides BEFORE they are used is the normal and logical practice. In this way the slides can be ready beforehand. The thing is that if the person using the slides does NOT pay attention, the "misslabelling" can occur. On the other hand the amount of time you could WASTE by leballing simultaneously while sectioning is astronomical. The good practice is to have a log with the blocks to section, prepare all the labelled slides in the needed amount and BEFORE sectioning the blocks, match slides to blocks and that is all you have to do. Sloppines and carelessnes are the roots of any mistake in histology. The solution is to pay attention, not to misuse a slides writing machine. Ren? J. Carmen Leschuk wrote: Recently, I heard of a slide mislabeling that occurred in another histo lab that they determined to be caused by pre-labeling slides before actual use at the microtome. This lab said that if the slides would of been labeled simultaneously (per their policy) as the blocks being cut, the mislabeling would of been prevented. My lab currently pre-labels all of slides before cutting a sequence of 10-20 blocks, which I thought was common practice. My question is, what is common practice? Do other labs have policies forbidding pre-labeling of glass slides? Carmen Leschuk, HT, SLS (ASCP) Supervisor, SJMO-Anatomic Pathology (248)858-6231 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for earth-friendly autos? Browse Top Cars by "Green Rating" at Yahoo! Autos' Green Center. From STapper <@t> slhduluth.com Mon Mar 12 12:05:11 2007 From: STapper <@t> slhduluth.com (Tapper, Sheila) Date: Mon Mar 12 12:05:24 2007 Subject: [Histonet] Pre-labeled slides Message-ID: There are many opportunities to create and "catch" an error. In the case of mislabeled slides, the microtomist, as well as the person who verified blocks to slides prior to distribution should share the responsibility for the error. The same mislabeling errors can occur if the slides are written individually as well, carelessness, or distractions are usually the reason. I am sure every lab has their process that best suits their workflow...but pre-labeled slides are more efficient than write-as-you-go. I have worked long enough (- not telling! ) to remember the pre-printer era. We are definitely more efficient now. Remember diamond pencils? Ouch! My wrist is hurting again! Sheila Tapper HT(ASCP) St. Luke's Hospital Duluth, MN 55805 From JMitchell <@t> uwhealth.org Mon Mar 12 12:25:29 2007 From: JMitchell <@t> uwhealth.org (Mitchell Jean A.) Date: Mon Mar 12 12:25:34 2007 Subject: [Histonet] 2007 Tri-State Symposium Message-ID: <936BDBD9AB6ED84FB1FD25FD55DCDFB103558310@uwhis-xchng4.uwhis.hosp.wisc.edu> > You are invited to join the swash buckling histotechnology societies > of Iowa, Minnesota and Wisconsin as they host "Finding the Treasures > of > Histology" the 2007 Tri-State Symposium, April 25-27, at the Marriott > Hotel, West Des Moines, Iowa. > > All inclusive symposium registration fee of $100.00 (+ state > membership) covers workshops, seminars, lunches and our infamous > costume banquet. The 2007 "treasure hunting" banquet theme is: Ship > Wrecked in Des Moines; "Pirates of Histology - The Silver Curse" > > CEU Approved Workshops & Seminars offered: > > Thursday April 26th: The Joy of Histology or What We Can Learn from > the Kitchen, Basic Tissue Gross Dissection & Description, Ready or Not > Here it Comes; Microwave Technology, Animal Tissue STAT-IHC Staining, > Frozen Section Techniques & New Cryostat Methods > > Friday April 27th: Expanding Histotechnology Services to Encompass > Animal-Based Translational Research, How Laboratory Safety Effects > You, Avian Influenza; A Global Perspective, Cliff Notes for Tissue > Identification, Preparing for the CAP Inspection, Histology Quality > Improvement Program (HQIP): What is it & How can it be Used > Effectively in the Laboratory, Standardization & Quality Control of > IHC > > For further information contact any of the Tri-State presidents: > > Iowa - Dave Cavanaugh (dcavanau@iastate.edu) > Minnesota - Colleen Forster (cforster@umn.edu) > Wisconsin - Maureen Decorah (decorah@rarc.wisc.edu) From POWELL_SA <@t> Mercer.edu Mon Mar 12 14:31:42 2007 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Mon Mar 12 14:33:43 2007 Subject: [Histonet] Georgia histotechs and students Message-ID: <01ME4WZXE8BM8WWHGD@Macon2.Mercer.edu> The Georgia Society for Histotechnologists Awards Committee is soliciting nominations for our two student awards. They are described below. 1. The GSH Holde Puchtler Student Award is given to a deserving student that exemplifies academic ability and outstanding qualities in histological techniques. The student should be currently enrolled in an approved School of Histotechnology and graduating in the current year, 2007. The $250 monetary award is to be used exclusively to attend a GSH, NSH or Region III meeting and will expire 2 years after award presentation. The student will be refunded up to $250 on providing original receipts for that meeting. 2. The GSH Student of the Year Award is for a deserving student in an approved School of Histotechnology that exemplifies academic ability and outstanding qualities in histological techniques. The student should be graduating the current year, 2007. Contact me for the forms to complete and return by March 31, 2007 to: Patti Wilson GSH Awards Co-Chair 4459 Westfield Drive Mableton, GA 30126 From FUNKM <@t> mercyhealth.com Mon Mar 12 15:26:51 2007 From: FUNKM <@t> mercyhealth.com (Marcia Funk) Date: Mon Mar 12 15:27:22 2007 Subject: [Histonet] Histology Lab Message-ID: <45F5713B020000AC0000044A@nodcmsngwia1.trinity-health.org> Hello, If there was a perfect Histology Lab what would it be like ? Does anyone know if there are plans published for the perfect Histology lab. thanks From boligy <@t> musc.edu Mon Mar 12 15:31:35 2007 From: boligy <@t> musc.edu (Yuriko Bolig) Date: Mon Mar 12 15:32:07 2007 Subject: [Histonet] HTL Program Message-ID: For those of you who are interested in a HTL program, the MUSC Medical Center offers one of three NAACLS accredited programs. The program lasts 12 months and offers a Post Bachelor's Histotechnology Certificate. The curriculum consists of lectures, individualized instruction and training in our medical center laboratories. For complete details and further information visit us on the web at: www.musc.edu/histoprogram Contact info: Nina Epps, BS, M.S. HPE, MT(ASCP) Histotechnolgy Program Director Laboratory Services Medical University of South Carolina Medical Center 165 Ashley Avenue P.O. Box 250908 Charleston, SC 29425 843-792-1906 E-mail: eppsn@musc.edu From rjbuesa <@t> yahoo.com Mon Mar 12 15:49:25 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 12 15:49:33 2007 Subject: [Histonet] Histology Lab In-Reply-To: <45F5713B020000AC0000044A@nodcmsngwia1.trinity-health.org> Message-ID: <161054.44657.qm@web61222.mail.yahoo.com> Marcia: There is no such a thing as a "perfect histology lab". Each has to be designed around the work type and expected volume, the investment level and the desired technology and, as you can imagine, all those variables differ from one to other setting. If by "plans" you refer to the blue-prints, that also has to vary with the work flow you expect, even if you want to get involved in any sort of "lean" approach technology or work flow.. Ren? J. Marcia Funk wrote: Hello, If there was a perfect Histology Lab what would it be like ? Does anyone know if there are plans published for the perfect Histology lab. thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- We won't tell. Get more on shows you hate to love (and love to hate): Yahoo! TV's Guilty Pleasures list. From doug <@t> ppspath.com Mon Mar 12 15:52:26 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Mon Mar 12 15:50:26 2007 Subject: [Histonet] Histology Lab In-Reply-To: <45F5713B020000AC0000044A@nodcmsngwia1.trinity-health.org> Message-ID: Perfect and Histology should never be used in the same sentence. :) Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcia Funk Sent: Monday, March 12, 2007 4:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology Lab Hello, If there was a perfect Histology Lab what would it be like ? Does anyone know if there are plans published for the perfect Histology lab. thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lchausse <@t> nmh.org Mon Mar 12 16:30:29 2007 From: Lchausse <@t> nmh.org (Chaussey, Leslie) Date: Mon Mar 12 16:30:56 2007 Subject: [Histonet] Histology Lab In-Reply-To: <161054.44657.qm@web61222.mail.yahoo.com> Message-ID: I agree with Ren?. However, I was recently asked to investigate if there is any published data that addresses a recommended gross room size (square footage) relative to the number of cases and/or specimens received. Is anyone aware of any benchmarking that deals with that question? Thanks for your help. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, March 12, 2007 3:49 PM To: Marcia Funk; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Histology Lab Marcia: There is no such a thing as a "perfect histology lab". Each has to be designed around the work type and expected volume, the investment level and the desired technology and, as you can imagine, all those variables differ from one to other setting. If by "plans" you refer to the blue-prints, that also has to vary with the work flow you expect, even if you want to get involved in any sort of "lean" approach technology or work flow.. Ren? J. Marcia Funk wrote: Hello, If there was a perfect Histology Lab what would it be like ? Does anyone know if there are plans published for the perfect Histology lab. thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- We won't tell. Get more on shows you hate to love (and love to hate): Yahoo! TV's Guilty Pleasures list. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- This message and any included attachments are intended only for the addressee. The information contained in this message is confidential and may constitute proprietary or non-public information under international, federal, or state laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail. From jnocito <@t> satx.rr.com Mon Mar 12 17:37:12 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Mon Mar 12 17:36:57 2007 Subject: [Histonet] Histology Lab References: <45F5713B020000AC0000044A@nodcmsngwia1.trinity-health.org> Message-ID: <00ee01c764f6$fa9a5820$649eae18@yourxhtr8hvc4p> one without pathologists. Oops, did I say that out loud? Is it Friday yet? JTT ----- Original Message ----- From: "Marcia Funk" To: Sent: Monday, March 12, 2007 3:26 PM Subject: [Histonet] Histology Lab Hello, If there was a perfect Histology Lab what would it be like ? Does anyone know if there are plans published for the perfect Histology lab. thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From debbiekeith <@t> cox.net Mon Mar 12 17:43:48 2007 From: debbiekeith <@t> cox.net (Debbie Keith) Date: Mon Mar 12 17:43:58 2007 Subject: [Histonet] Histology Lab In-Reply-To: <45F5713B020000AC0000044A@nodcmsngwia1.trinity-health.org> Message-ID: <5.2.0.9.0.20070312153905.02b94420@pop.central.cox.net> At 04:26 PM 3/12/2007 -0400, you wrote: >Hello, > >If there was a perfect Histology Lab what would it be like ? Does anyone >know if there are plans published >for the perfect Histology lab. > >thanks given the fact that nearly every tech (the ones i know) are afflicted with a WICKED case of OCD.... everyone's definition of the "perfect" histology lab would be very different. some folks LOVE open storage, others find it offensive. some like an open floorplan.... others like nooks. (i agree with Joe!) there should be a mode of request/slide delivery that minimizes contact between techs/pathologists. ;) (like those funny chute sytems at the drive-up tellers?) ;) frankly, the floor should be durable/impervious to chemicals (and the clumsy tech). i'm thinking sealed concrete with a drain in the middle. think kill-floor at the slaughterhouse. fitting. no? ;) deb -- No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.5.446 / Virus Database: 268.18.9/719 - Release Date: 3/12/2007 8:41 AM From alanb <@t> medlabcentral.co.nz Mon Mar 12 20:55:37 2007 From: alanb <@t> medlabcentral.co.nz (Alan Bishop) Date: Mon Mar 12 21:03:11 2007 Subject: [Histonet] Leica IPC cassette printer Message-ID: Anyone got any feedback on the IPC cassette printer from Leica? We have had ours a grand total of 3 weeks and it's just gone belly up with a fatal error!! Apparently the power supply and flash unit are buggered and I was just wondering how often this might be happening around the world as it seems to be a known fault? Cheers Alan Alan Bishop Charge scientist Histology Medlab Central Palmerston North Tel: 06 952 3135 Fax: 06 952 3199 From katri <@t> cogeco.ca Mon Mar 12 21:35:38 2007 From: katri <@t> cogeco.ca (Katri Tuomala) Date: Mon Mar 12 21:35:46 2007 Subject: [Histonet] Pre-labeling glass slides References: <893017.12787.qm@web61221.mail.yahoo.com> Message-ID: <002601c76518$496450c0$6a9a9618@Katri> I totally agree with Rene. We pre-label all our slides in approximately 20 slide lots. We have so many checks along the way, before the slides are delivered, that the odd mistake gets discovered very quickly. You just have to pay attention to what you are doing. And yes, I remember the diamond pens, ouch!! Katri Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "Rene J Buesa" To: "Carmen Leschuk" ; Sent: Monday, March 12, 2007 12:44 PM Subject: Re: [Histonet] Pre-labeling glass slides > Carmen: > Labelling the slides BEFORE they are used is the normal and logical > practice. In this way the slides can be ready beforehand. The thing is > that if the person using the slides does NOT pay attention, the > "misslabelling" can occur. > On the other hand the amount of time you could WASTE by leballing > simultaneously while sectioning is astronomical. > The good practice is to have a log with the blocks to section, prepare > all the labelled slides in the needed amount and BEFORE sectioning the > blocks, match slides to blocks and that is all you have to do. > Sloppines and carelessnes are the roots of any mistake in histology. The > solution is to pay attention, not to misuse a slides writing machine. > Ren? J. > > Carmen Leschuk wrote: > Recently, I heard of a slide mislabeling that occurred in another histo > lab that they determined to be caused by pre-labeling slides before actual > use at the microtome. This lab said that if the slides would of been > labeled simultaneously (per their policy) as the blocks being cut, the > mislabeling would of been prevented. My lab currently pre-labels all of > slides before cutting a sequence of 10-20 blocks, which I thought was > common practice. My question is, what is common practice? Do other labs > have poli > cies forbidding pre-labeling of glass slides? > > > > Carmen Leschuk, HT, SLS (ASCP) > Supervisor, SJMO-Anatomic Pathology > (248)858-6231 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Looking for earth-friendly autos? > Browse Top Cars by "Green Rating" at Yahoo! Autos' Green Center. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nfournier <@t> sasktel.net Tue Mar 13 02:59:07 2007 From: nfournier <@t> sasktel.net (Neil Fournier) Date: Tue Mar 13 02:59:18 2007 Subject: [Histonet] where do you purchase normal sera and cresyl violet Message-ID: <004f01c76545$7a61ff60$20ffc5d8@NEIL> Hi everyone, I was wondering where people typically purchase normal sera that they use as blocking agents for IHC (for example on fixed rat brain). We purchased normal animal sera through Vector Laboratories; however, considering how much we usually go through (we do a lot of our procedures as free-floating) and the rather expensive costs I was wondering if any one knew of cheaper places that still yield high quality products. Vector sells 10 ml to us for over $150 after shipping and exchange rate and I have noticed that Invitrogen sells an equivalent product (normal goat sera) which consists of 100 ml and costs around $30.00. I am not sure what the specific differences between the two are but this is a huge difference in price and I am not sure if it translate into anything meaningful in terms of quality of blocking. And lastly, I typically buy cresyl violet acetate through Acros. We have had beautiful results but once again it is a little on the pricey side considering the small quantity we purchase. I once tried a different product (not sure what it was) and it was useless (in fact the solution wouldn't even stain my own fingers). Once again does anyone have any product suggestions that have worked well for them in the past. I hate changing procedures that already work well but if we can cut some costs then perhaps I can argue for a nice pay increase or holiday in Florida. Thanks for the advice, Neil From ree3 <@t> leicester.ac.uk Tue Mar 13 04:20:29 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Tue Mar 13 04:20:40 2007 Subject: [Histonet] Histology Lab In-Reply-To: <00ee01c764f6$fa9a5820$649eae18@yourxhtr8hvc4p> References: <00ee01c764f6$fa9a5820$649eae18@yourxhtr8hvc4p> Message-ID: It is the people that make any place of work perfect or otherwise, achieving the optimum blend of ages, sex and experience of the workers is largely a matter of luck. Sent: Monday, March 12, 2007 3:26 PM Subject: [Histonet] Histology Lab Hello, If there was a perfect Histology Lab what would it be like ? Does anyone know if there are plans published for the perfect Histology lab. thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kappeler <@t> patho.unibe.ch Tue Mar 13 05:08:37 2007 From: kappeler <@t> patho.unibe.ch (Andi Kappeler) Date: Tue Mar 13 05:08:56 2007 Subject: [Histonet] where do you purchase normal sera and cresyl violet References: <004f01c76545$7a61ff60$20ffc5d8@NEIL> Message-ID: <00bc01c76557$91798990$27955c82@patho.unibe.ch> Hi Neil we have been buying our sera for IHC (goat, rabbit, swine, horse, ...) for years from Invitrogen (formerly Gibco Life Technologies) with good results. We thaw the 500 ml bottles, aliquot them and store the aliquots in the freezer until we need them. Invitrogen supplies sera for cell culture and similar purposes, that's probably why you get reasonable volumes at affordable prices. Immuno companies probably sell (hopefully) the same quality but repacked in tiny little bottles ... Hope this helps Andi Kappeler Institute of Pathology, University of Bern, Switzerland ----- Original Message ----- From: "Neil Fournier" To: Sent: Tuesday, March 13, 2007 8:59 AM Subject: [Histonet] where do you purchase normal sera and cresyl violet > Hi everyone, > > I was wondering where people typically purchase normal sera that they use > as blocking agents for IHC (for example on fixed rat brain). We > purchased normal animal sera through Vector Laboratories; however, > considering how much we usually go through (we do a lot of our procedures > as free-floating) and the rather expensive costs I was wondering if any > one knew of cheaper places that still yield high quality products. Vector > sells 10 ml to us for over $150 after shipping and exchange rate and I > have noticed that Invitrogen sells an equivalent product (normal goat > sera) which consists of 100 ml and costs around $30.00. I am not sure > what the specific differences between the two are but this is a huge > difference in price and I am not sure if it translate into anything > meaningful in terms of quality of blocking. > > And lastly, I typically buy cresyl violet acetate through Acros. We have > had beautiful results but once again it is a little on the pricey side > considering the small quantity we purchase. I once tried a different > product (not sure what it was) and it was useless (in fact the solution > wouldn't even stain my own fingers). Once again does anyone have any > product suggestions that have worked well for them in the past. > > I hate changing procedures that already work well but if we can cut some > costs then perhaps I can argue for a nice pay increase or holiday in > Florida. > > Thanks for the advice, > > Neil > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From TMcNemar <@t> lmhealth.org Tue Mar 13 07:28:10 2007 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Tue Mar 13 06:28:07 2007 Subject: [Histonet] Pre-labeling glass slides In-Reply-To: <45F545630200001500283F50@nodcmsngwia1.trinity-health.org> Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F469@lmhsmail.lmhealth.org> We do not pre-label slides just for that reason. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Carmen Leschuk Sent: Monday, March 12, 2007 11:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pre-labeling glass slides Recently, I heard of a slide mislabeling that occurred in another histo lab that they determined to be caused by pre-labeling slides before actual use at the microtome. This lab said that if the slides would of been labeled simultaneously (per their policy) as the blocks being cut, the mislabeling would of been prevented. My lab currently pre-labels all of slides before cutting a sequence of 10-20 blocks, which I thought was common practice. My question is, what is common practice? Do other labs have policies forbidding pre-labeling of glass slides? Carmen Leschuk, HT, SLS (ASCP) Supervisor, SJMO-Anatomic Pathology (248)858-6231 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ryakay <@t> shands.ufl.edu Tue Mar 13 07:45:13 2007 From: ryakay <@t> shands.ufl.edu (Kaye Ryan) Date: Tue Mar 13 07:45:30 2007 Subject: [Histonet] Glassware cleaning Message-ID: <45F66499020000D800002291@gw-fs1.shands.ufl.edu> Hi Everyone, According to the General CAP Checklist (GEN.41770) "documented procedures for handling and cleaning of glassware, including methods for testing for detergent removal" must be performed. An example of a method for doing this is given. Can you please tell me how you are documenting this for the histology lab? We do have a procedure for doing this but I wanted to see how others are documenting this. Thanks in advance, Kaye Ryan Kaye Ryan Histology Manager/Educational Coordinator Shands Rocky Point Laboratories (352) 265-0111, 72093 From RohrT <@t> nyackhospital.org Tue Mar 13 09:00:18 2007 From: RohrT <@t> nyackhospital.org (Theresa Rohr) Date: Tue Mar 13 08:03:06 2007 Subject: [Histonet] CD56 (123C3.D5) Message-ID: Dear Histonetters, Is anyone out there using this antibody, CD56, from Cell Marque with the Dako Envision+ protocol. I am having some trouble arriving at a good dilution. Am presently at 1:100 with 20 min TR w/EDTA Buffer, 30 in antibody, 30 in polymer and 10 min DAB. Some membranous staining but not really crisp and a whole lot of background staining. My control is hemorrhagic brain tissue, the only sizeable example of small cell I could find in our history. We tried carcinoid, astrocytoma and some autopsy material but the docs were not happy. What else is new???????? They want to see it on small cell and this brain mets case is the only sizeable tumor. Does anyone have a better protocol with Envision+ and also is there another way for me to quench that background staining. I increased my H2O2 from 5 to 10 minutes and got no better results at all? Can I use Pepsin too????? I would appreciate any advice. And yes, I did contact Cell Marque and am waiting for their suggestions too. But it's my peers that I trust the most!!!!!!!!!! Thanks so much, Theresa Rohr, Nyack Hospital rohrt@nyackhospital.org Theresa Rohr, BA, HT(ASCP) Section Head, Histology Nyack Hospital 160 North Midland Avenue Nyack, New York 10960 phone 845-348-2276 fax 845-348-8430 Nyack Hospital is required by Law to protect the privacy of health information under HIPAA Rules and Regulations Part 11, Article 45 CFR, Parts 160-164. This email, including attachments, is intended for the exclusive use of the person or the entity to which it is addressed. It may contain confidential or legally protected information. The authorized recipient is prohibited from disclosing this information to any other party and is required to destroy this information after its stated need has been fulfilled. If the recipient or reader of this email is not the intended recipient or her/his authorized agent, the reader is hereby notified that any dissemination, distribution or copying of this email is prohibited. If you believe that you have received this email in error, please advise the sender immediately by reply email and then delete this email immediately. From hej01 <@t> health.state.ny.us Tue Mar 13 08:08:55 2007 From: hej01 <@t> health.state.ny.us (Helen E Johnson) Date: Tue Mar 13 08:09:10 2007 Subject: [Histonet] mouse on mouse kit Message-ID: Hi Histonetters, I need a recommendation on a IHC mouse on mouse detection kit. Helen Johnson (hej01@health.state.ny.us) From rjr6 <@t> psu.edu Tue Mar 13 08:11:44 2007 From: rjr6 <@t> psu.edu (Roberta Horner) Date: Tue Mar 13 08:11:53 2007 Subject: [Histonet] Pre-labeling glass slides In-Reply-To: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F469@lmhsmail.lmhealth.org> References: <45F545630200001500283F50@nodcmsngwia1.trinity-health.org> <51D5D78FBEDAEA4FBCCD9A9D44211DC528F469@lmhsmail.lmhealth.org> Message-ID: I have to pre-label my slides. I am the only tech and in the morning time is tight and I can get everything cut if I don't have to write the slides then. I also have several checks along the way if I would pick something up on the wrong slide. I work in a veterinary diagnostic lab so errors though critical are not quite as bad as in a human hospital. In 20 years I may have mislabeled something once that I did not catch but the pathologist did. Roberta Horner HT, HTL Animal Diagnostic Lab Penn State University From lpaveli1 <@t> hurleymc.com Tue Mar 13 08:07:47 2007 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Tue Mar 13 08:12:37 2007 Subject: [Histonet] procedure for disposal of DAB waste from Ventana Stainer Message-ID: <45F65BD4020000EE000123AC@smtp-gw.hurleymc.com> Tiffany, I am quoting an exerpt from Dapson & Dapson's (Anatech) Hazardous Materials in the Histopathology Laboratory (3rd edition of Regulations Risks Handling and Disposal): "The chromogens AEC and DAB likewise have been the subject of much misinformation. DAB is probably carcinogenic, based on its relationship to the benzidine family (all members of which that have been studied have been shown to be carcinogenic). AEC has been suggested as a safer alternative, but toxicological literature has neither supported nor refuted that claim to date. While there is no detoxification process for AEC, two exist for DAB. Do not use sodium hypochlorite (household bleach) as a detoxifying agent for DAB, despite recommendations to the contrary in some product literature, because the reaction products remain mutagenic. Lunn and Sansone present two methods for detoxifying DAB. One involves the use of acidified potassium permanganate to detoxify the chromogen, then sodium ascorbate and sodium bicarbonate, respectively to decolorize and nutralize the mixture prior to drain disposal. This method works for the solutions of DAB as well as the colored precipitate that forms when DAB is reacted with horseradish peroxidase (the latter is mutagenic like DAB)." Procedure: 1. Prepare the following aqueous stock solutions a. 0.2 M potassium permanganate (31.6 g KMnO4/liter) b. 2.0 M sulfuric acid (112 ml concentrated aced/liter) 2. Dilute the DAB solution if necessary so that its concentration does not exceed 0.9 mg/ml. 3. For each 10 ml of DAB solution, add: a. 5 ml 0.2 M potassium permanganate b. 5 ml 2.0 M sulfuric acid 4. Allow mixture to stand for at least 10 hours. It is now non-mutagenic. 5. Decolorize the mixture with ascorbic acid (add powder until color disappears). 6. Nutralize the decolorized mixture with sodium bicarbonate (test with pH meter, pH paper or dipsticks). 7. Discard the solution down the drain provided that local wastewater authorities have given their approval. Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810-257-9948 fax: 810-762-7082 >>> "Price, Tiffany" 03/07/07 11:08 AM >>> Does anyone have a procedure for disposal of DAB waste? I have a procedure that uses magnesium permanganate, but I understand that you can also use bleach. Thanks for any information- Tiffany Confidentiality Notice: This email message including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privilege information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy ALL copies of the message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Tue Mar 13 09:29:07 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Mar 13 09:29:15 2007 Subject: [Histonet] where do you purchase normal sera and cresyl violet In-Reply-To: <004f01c76545$7a61ff60$20ffc5d8@NEIL> References: <004f01c76545$7a61ff60$20ffc5d8@NEIL> Message-ID: <6.0.0.22.1.20070313081338.01b56640@gemini.msu.montana.edu> VWR sells normal serums in huge lots, and if you can get a discount, even more savings. It is Animal Sera, from SeraCare Life Sciences, and purchased via VWR. Example goat serum, cat#14231-814, 500 ml list price is $96.54. We realiquot into 50 ml tubes and store at -27C. 100 ml mouse serum is only $81.68 list. Check out their and other company websites for similar items in product category serum, cell culture If you have any kind of term contract with a large company (VWR, Fisher) you may get free shipping. Sigma has huge lots but it is not cheaper, comes frozen, and is expensive shipping. We purchase donkey serum from Jackson, it comes lyophilized in 10 ml sizes, is stable in that condition at 4C for years (they said 10 years!) They do not break the bank with their shipping charges. You may be able to work a deal with them to buy this in larger quantity, Jackson has been a great company to deal with. Dyes are always expensive and you may bite the bullet on these. If you have a term contract with Fisher, then buying through ACROS should be discounted, with any special shipping deals you have made with them. We purchase from Sigma-Aldrich. Good luck At 01:59 AM 3/13/2007, you wrote: >Hi everyone, > >I was wondering where people typically purchase normal sera that they use >as blocking agents for IHC (for example on fixed rat brain). We >purchased normal animal sera through Vector Laboratories; however, >considering how much we usually go through (we do a lot of our procedures >as free-floating) and the rather expensive costs I was wondering if any >one knew of cheaper places that still yield high quality products. Vector >sells 10 ml to us for over $150 after shipping and exchange rate and I >have noticed that Invitrogen sells an equivalent product (normal goat >sera) which consists of 100 ml and costs around $30.00. I am not sure >what the specific differences between the two are but this is a huge >difference in price and I am not sure if it translate into anything >meaningful in terms of quality of blocking. > >And lastly, I typically buy cresyl violet acetate through Acros. We have >had beautiful results but once again it is a little on the pricey side >considering the small quantity we purchase. I once tried a different >product (not sure what it was) and it was useless (in fact the solution >wouldn't even stain my own fingers). Once again does anyone have any >product suggestions that have worked well for them in the past. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From gcallis <@t> montana.edu Tue Mar 13 09:39:16 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Mar 13 09:39:28 2007 Subject: Recommendation for Re: [Histonet] mouse on mouse kit In-Reply-To: References: Message-ID: <6.0.0.22.1.20070313083135.01b77c18@gemini.msu.montana.edu> Helen, A wonderful poster at the NSH symposium/convention in Ft Lauderdale (by Mary Payette, et al) showed a comparison of 8 kits. The top two giving no background were DAKO's ARK kit and Biocares MM kit, both based on biotinylation of the primary antibody. One had minimal background, two gave moderate background, and three had marked background. This was a very well designed test of kits. In our experience, kits containing secondary antibodies always gave more background, even with all the tidy little special blocks companies put in the kits. We would be using the top two above in the future. 07:08 AM 3/13/2007, you wrote: >Hi Histonetters, > I need a recommendation on a IHC mouse on mouse detection kit. > Helen Johnson Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From doug <@t> ppspath.com Tue Mar 13 10:49:28 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue Mar 13 09:47:25 2007 Subject: [Histonet] Job Opening: Pathologists Assistant Message-ID: Professional Pathology Services, PC is one of the regions fastest growing Pathology laboratories. It is located in sunny Columbia South Carolina in heart of Carolina Research Park. We are located in the middle of the state, 1 ? hours from beach and mountains, with a large state university and a wide variety of cultural events. Our pathology laboratory features a state-of-the-art Histology and Cytology laboratory. We also have a wall of windows with a scenic view of our own pond. Full benefits, salary negotiable. Come join our fun and exciting team of health care professionals. We are seeking a full-time Certified Pathologists Assistant for a late afternoon shift. This position is for grossing duties (no autopsies). Visit our website at www.ppspath.com. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. From slappycraw <@t> yahoo.com Tue Mar 13 10:18:49 2007 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Tue Mar 13 10:19:01 2007 Subject: [Histonet] Histology Lab In-Reply-To: Message-ID: <807163.73987.qm@web53614.mail.yahoo.com> I'm really tempted to reply to that last message but I just can't seem to get in the right frame of mind. "Edwards, R.E." wrote: It is the people that make any place of work perfect or otherwise, achieving the optimum blend of ages, sex and experience of the workers is largely a matter of luck. Sent: Monday, March 12, 2007 3:26 PM Subject: [Histonet] Histology Lab Hello, If there was a perfect Histology Lab what would it be like ? Does anyone know if there are plans published for the perfect Histology lab. thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Finding fabulous fares is fun. Let Yahoo! FareChase search your favorite travel sites to find flight and hotel bargains. From rjbuesa <@t> yahoo.com Tue Mar 13 10:26:37 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 13 10:26:48 2007 Subject: [Histonet] Glassware cleaning In-Reply-To: <45F66499020000D800002291@gw-fs1.shands.ufl.edu> Message-ID: <11514.89046.qm@web61225.mail.yahoo.com> Prepare a log with date the cleaning was performed; test for alkalinity used, and initials of the person doing both the cleaning and the test. Keep that log available for inspection. Ren? J. Kaye Ryan wrote: Hi Everyone, According to the General CAP Checklist (GEN.41770) "documented procedures for handling and cleaning of glassware, including methods for testing for detergent removal" must be performed. An example of a method for doing this is given. Can you please tell me how you are documenting this for the histology lab? We do have a procedure for doing this but I wanted to see how others are documenting this. Thanks in advance, Kaye Ryan Kaye Ryan Histology Manager/Educational Coordinator Shands Rocky Point Laboratories (352) 265-0111, 72093 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Food fight? Enjoy some healthy debate in the Yahoo! Answers Food & Drink Q&A. From rjbuesa <@t> yahoo.com Tue Mar 13 10:30:27 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 13 10:30:36 2007 Subject: [Histonet] CD56 (123C3.D5) In-Reply-To: Message-ID: <327867.53180.qm@web61212.mail.yahoo.com> Theresa: I used CD56 from NovoCastra diluted 1:20 with HIER at pH6 DAKO detection systems and brain control with good results. Ren? J. Theresa Rohr wrote: Dear Histonetters, Is anyone out there using this antibody, CD56, from Cell Marque with the Dako Envision+ protocol. I am having some trouble arriving at a good dilution. Am presently at 1:100 with 20 min TR w/EDTA Buffer, 30 in antibody, 30 in polymer and 10 min DAB. Some membranous staining but not really crisp and a whole lot of background staining. My control is hemorrhagic brain tissue, the only sizeable example of small cell I could find in our history. We tried carcinoid, astrocytoma and some autopsy material but the docs were not happy. What else is new???????? They want to see it on small cell and this brain mets case is the only sizeable tumor. Does anyone have a better protocol with Envision+ and also is there another way for me to quench that background staining. I increased my H2O2 from 5 to 10 minutes and got no better results at all? Can I use Pepsin too????? I would appreciate any advice. And yes, I did contact Cell Marque and am waiting for their suggestions too. But it's my peers that I trust the most!!!!!!!!!! Thanks so much, Theresa Rohr, Nyack Hospital rohrt@nyackhospital.org Theresa Rohr, BA, HT(ASCP) Section Head, Histology Nyack Hospital 160 North Midland Avenue Nyack, New York 10960 phone 845-348-2276 fax 845-348-8430 Nyack Hospital is required by Law to protect the privacy of health information under HIPAA Rules and Regulations Part 11, Article 45 CFR, Parts 160-164. This email, including attachments, is intended for the exclusive use of the person or the entity to which it is addressed. It may contain confidential or legally protected information. The authorized recipient is prohibited from disclosing this information to any other party and is required to destroy this information after its stated need has been fulfilled. If the recipient or reader of this email is not the intended recipient or her/his authorized agent, the reader is hereby notified that any dissemination, distribution or copying of this email is prohibited. If you believe that you have received this email in error, please advise the sender immediately by reply email and then delete this email immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Need Mail bonding? Go to the Yahoo! Mail Q&A for great tips from Yahoo! Answers users. From douglas_moore <@t> brown.edu Tue Mar 13 11:30:17 2007 From: douglas_moore <@t> brown.edu (Douglas Moore) Date: Tue Mar 13 11:30:27 2007 Subject: [Histonet] Odd Black Staining in Plastic Embedded Bone Samples Message-ID: I am looking for possible explanations for some strange black staining on some plastic embedded bone slides stained with Stevenel's blue stain. The specimens are from the distal femur of the New Zealand white rabbit, and we were evaluating the resorption of a biomaterial (CaP, collagen and carboxymethyl cellulose). I have posted a seres of four images on histonet.org (bonegraft_overview.jpg, bonegraft_detail.jpg, biomaterial_overview.jpg, biomaterial_detail.jpg) We're perplexed--never seen anything like this before. The strange thing is, we see the black staining in our autograft bone controls, too. Any help would be appreciated, Doug -- __________________________________________________ Douglas C. Moore, M.S. Assoc. Dir. Bioengineering Laboratory Sr. Research Associate, Department of Orthopaedics Brown Medical School/Rhode Island Hospital Bioengineering Laboratory CORO West, Suite 404 1 Hoppin Street Providence, RI 02903 Ph: 401.444.8904 Fax: 401.444.4418 email: douglas_moore@brown.edu From TJJ <@t> Stowers-Institute.org Tue Mar 13 11:50:14 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Tue Mar 13 11:50:45 2007 Subject: [Histonet] Re: perfect histology lab Message-ID: I beg to differ. As anybody who has visited my lab can attest, I think I have the perfect histology lab. =) Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From scorpionrider <@t> cox.net Tue Mar 13 12:16:37 2007 From: scorpionrider <@t> cox.net (scorpionrider@cox.net) Date: Tue Mar 13 12:16:47 2007 Subject: [Histonet] Re: perfect histology lab Message-ID: <12863704.1173806197724.JavaMail.root@fed1wml02.mgt.cox.net> Teri, Would that be a one-person lab you have there?? :-) ---- "Johnson wrote: > I beg to differ. As anybody who has visited my lab can attest, I think I > have the perfect histology lab. =) > > Teri Johnson, HT(ASCP)QIHC > Managing Director Histology Facility > Stowers Institute for Medical Research > 1000 E. 50th St. > Kansas City, MO 64110 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SLB <@t> Stowers-Institute.org Tue Mar 13 12:41:59 2007 From: SLB <@t> Stowers-Institute.org (Beckham, Sharon) Date: Tue Mar 13 12:42:36 2007 Subject: [Histonet] Re: perfect histology lab In-Reply-To: <12863704.1173806197724.JavaMail.root@fed1wml02.mgt.cox.net> Message-ID: I am one of five people who work in Teri's lab and it the perfect histology lab!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of scorpionrider@cox.net Sent: Tuesday, March 13, 2007 12:17 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: perfect histology lab Teri, Would that be a one-person lab you have there?? :-) ---- "Johnson wrote: > I beg to differ. As anybody who has visited my lab can attest, I think > I have the perfect histology lab. =) > > Teri Johnson, HT(ASCP)QIHC > Managing Director Histology Facility > Stowers Institute for Medical Research > 1000 E. 50th St. > Kansas City, MO 64110 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ljwh2 <@t> cam.ac.uk Tue Mar 13 12:44:05 2007 From: ljwh2 <@t> cam.ac.uk (Laura Harris) Date: Tue Mar 13 12:45:15 2007 Subject: [Histonet] mouse on mouse kit Message-ID: <45F6E2E5.4060903@cam.ac.uk> I used Vector's mouse-on-mouse kit on several antibodies on frozen tissue, with no appreciable reduction of the background at all! I found it to be a complete waste of time. Laura Harris University of Cambridge, UK Date: Tue, 13 Mar 2007 08:39:16 -0600 From: Gayle Callis Subject: Recommendation for Re: [Histonet] mouse on mouse kit To: Helen E Johnson , Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20070313083135.01b77c18@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Helen, A wonderful poster at the NSH symposium/convention in Ft Lauderdale (by Mary Payette, et al) showed a comparison of 8 kits. The top two giving no background were DAKO's ARK kit and Biocares MM kit, both based on biotinylation of the primary antibody. One had minimal background, two gave moderate background, and three had marked background. This was a very well designed test of kits. In our experience, kits containing secondary antibodies always gave more background, even with all the tidy little special blocks companies put in the kits. We would be using the top two above in the future. 07:08 AM 3/13/2007, you wrote: > >Hi Histonetters, > > I need a recommendation on a IHC mouse on mouse detection kit. > > Helen Johnson Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From scorpionrider <@t> cox.net Tue Mar 13 12:50:39 2007 From: scorpionrider <@t> cox.net (scorpionrider@cox.net) Date: Tue Mar 13 12:50:47 2007 Subject: [Histonet] Re: perfect histology lab Message-ID: <26392625.1173808239781.JavaMail.root@fed1wml02.mgt.cox.net> Excellent. I have worked in small and large labs and found good and bad in each. I do believe that the people are what make for the perfect work experience. Mark ---- "Beckham wrote: > I am one of five people who work in Teri's lab and it the perfect > histology lab!!! > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > scorpionrider@cox.net > Sent: Tuesday, March 13, 2007 12:17 PM > To: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Re: perfect histology lab > > > > Teri, > > Would that be a one-person lab you have there?? :-) > > > ---- "Johnson wrote: > > I beg to differ. As anybody who has visited my lab can attest, I think > > > I have the perfect histology lab. =) > > > > Teri Johnson, HT(ASCP)QIHC > > Managing Director Histology Facility > > Stowers Institute for Medical Research > > 1000 E. 50th St. > > Kansas City, MO 64110 > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SBaldwin <@t> compucyte.com Tue Mar 13 12:16:21 2007 From: SBaldwin <@t> compucyte.com (Scott Baldwin) Date: Tue Mar 13 13:09:20 2007 Subject: [Histonet] Automated Quantitative Tissue Analysis Programs at UCSD and UCLA Message-ID: Dear Histonetters, This is just a quick note to the research pathology community in the San Diego and LA areas and all who are interested in trying out state-of-the-art automated tissue analysis capabilities of the new generation laser scanning cytometers. We will be offering free sample evaluation clinics. Instrumentation will be available at UCSD, Moores Cancer Center Imaging Core (from March 30 until April 5) and UCLA, the Molecular Screening Shared Resource (from April 9 until April 13), CompuCyte scientists will perform sample analysis and provide consultations. Fluorescent and chromatic dyes, or combination of both, can be used for quantification of tissue constituent expression. There are no specific requirements for sample preparation in addition to standard practices; most commonly used dyes, compatible with the instrument specifications are: Violet laser (405 nm) - DAPI, Hoechst 33342, and Qdot(tm) excitation. Blue laser (488 nm) - FITC, GFP, Alexa Fluor? 488, PE, and PE-Cy5 excitation. Propidium Iodide, DAB, BCIP absorption. Red laser (633 nm) - Cy5 and Alexa Fluor? 647 excitation. Hematoxylin, Nova Red and Methyl Green absorption. Additionally, there will be a presentation by Dr. William Geddie from the University of Toronto with specific focus on FNA immunophenotyping and tissue biomarkers for clinical trials (March 30 at UCSD, http://www.compucyte.com/Agenda%20-%20UCSD%20Symposium-2007.htm) and by Dr. Peter Gann on the subject of p27 expression analysis in prostate cancer prevention clinical trial (April 13 at UCLA, http://www.compucyte.com/Agenda%20-%20UCLA%20Symposium-2007.htm). Scott Baldwin MT(ASCP) CompuCyte From RRA <@t> Stowers-Institute.org Tue Mar 13 13:14:35 2007 From: RRA <@t> Stowers-Institute.org (Allen, Rhonda) Date: Tue Mar 13 13:15:00 2007 Subject: [Histonet] Perfect Histology Lab Message-ID: Teri and All, I have the perfect lab!! It is one person, and at Stower's! Teri, my boss, and the other 5, only see me at lunch, or if I visit! Rhonda Allen Rhonda Allen HTL(ASCP)QIHC Histology Specialist II Stowers Institute for Medical Research 1000 E. 50th Street Kansas City, MO 54110 816-926-4346 From TJJ <@t> Stowers-Institute.org Tue Mar 13 13:44:26 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Tue Mar 13 13:44:52 2007 Subject: [Histonet] RE: perfect histology lab In-Reply-To: Message-ID: Mark writes: Excellent. I have worked in small and large labs and found good and bad in each. I do believe that the people are what make for the perfect work experience.<< Mark, you're absolutely right, the personnel is a critical component. You get good, highly motivated and professional people, and that really sets the tone of the lab. I am also blessed with an adequate budget, excellent equipment, and a fantastic work environment. From George.Adams <@t> UTSouthwestern.edu Tue Mar 13 14:23:27 2007 From: George.Adams <@t> UTSouthwestern.edu (George Adams) Date: Tue Mar 13 14:23:42 2007 Subject: [Histonet] recommendation of ABC Kit Message-ID: <45F6B3DF02000018000137A1@swnw126.swmed.edu> Histonetters: Before I go into detail, I am aware of the most recent exchange re: avidin biotin complex (ABC) kits from Laura Harris and Gayle Callis (Histonet Digest, Vol. 40, Issue 17) and that Vector's mouse on mouse ABC kit is problematic while DAKO's ARK kit and Biocares MM kit seem to decent with regards to background. Are these results confined to only frozen sections? My plan was to do immunohistochemisty (IHC) of chondroitinase ABC-treated and untreated paraffin-embedded vocal fold tissues. Any opinions for this neophyte? George Adams From Eric.C.Kellar <@t> questdiagnostics.com Tue Mar 13 14:49:38 2007 From: Eric.C.Kellar <@t> questdiagnostics.com (Kellar, Eric C) Date: Tue Mar 13 15:07:50 2007 Subject: [Histonet] RE: Glassware cleaning Message-ID: <6843061CE6B98E4B96590D4F299618F801583C0D@qdcws0117.us.qdx.com> Hello Kaye, Here's my documentation procedure... 1. PROCEDURE FOR CLEANING GLASS/PLASTIC WARE Rinse all dishes with tap water thoroughly prior to washing. Use bleach and or appropriate strength detergent for stubborn stains prior to washing. Rinse thoroughly after acid cleaning. Wash dishes, either manually or by dishwasher. Rinse dishes thoroughly with DI or distilled water and dry dishes. 2. PROCEDURE FOR TESTING FOR RESIDUAL DETERGENT After washing and rinsing multiple pieces of glass/plastic ware, it is only necessary to randomly select one piece to test for residual detergent. Either one of the following two methods may be used. My lab aides rotate weekly through glassware cleaning and have both options. pH Meter Method Rinse a small clean beaker by filling and emptying 3 times with DI or distilled water. Fill the same beaker with DI or distilled water and measure the pH using a pH meter. Record the pH as "Source Water" pH on "Residual Detergent Testing Log" date and initial. Fill the piece of washed glass/plastic ware to be tested approximately 10% full with "source water" (i.e., 10ml into 100ml beaker). Add more "source water" if needed to completely immerse the pH meter electrode. Swirl "source water" in glass/plastic ware to extract detergent residues from all possible surfaces. Take pH reading with pH meter and record as "Glassware pH" on "Residual Detergent Testing Log" date and initial. Re-wash and rinse tested piece of glass/plastic ware before using Color Indicator Method Place 2.5 ml of DI or distilled water in the glass/plastic ware to be tested and swirl to extract detergent residues from all possible surfaces. Using a plastic pipette, add one drop of 0.5% Phenolphthalein indicator and look for a reddish color in the zone of first mixing. Do not mix vigorously. Record presence or absence of reddish color indicator on "Residual Detergent Testing Log" date and initial. Re-wash and rinse tested piece of glass/plastic ware before using. Eric C. Kellar Quest Diagnostics Histology Laboratory Supervisor South Florida -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kaye Ryan Sent: Tuesday, March 13, 2007 8:45 AM To: Histonet Subject: [Histonet] Glassware cleaning Hi Everyone, According to the General CAP Checklist (GEN.41770) "documented procedures for handling and cleaning of glassware, including methods for testing for detergent removal" must be performed. An example of a method for doing this is given. Can you please tell me how you are documenting this for the histology lab? We do have a procedure for doing this but I wanted to see how others are documenting this. Thanks in advance, Kaye Ryan Kaye Ryan Histology Manager/Educational Coordinator Shands Rocky Point Laboratories (352) 265-0111, 72093 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ============================================================================== The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. ============================================================================== From slappycraw <@t> yahoo.com Tue Mar 13 15:16:30 2007 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Tue Mar 13 15:16:41 2007 Subject: [Histonet] Re: perfect histology lab In-Reply-To: Message-ID: <308257.75784.qm@web53609.mail.yahoo.com> I have never visited your lab Teri but I have been to Kansas City... "Johnson, Teri" wrote: I beg to differ. As anybody who has visited my lab can attest, I think I have the perfect histology lab. =) Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- We won't tell. Get more on shows you hate to love (and love to hate): Yahoo! TV's Guilty Pleasures list. From George.Adams <@t> UTSouthwestern.edu Tue Mar 13 15:26:24 2007 From: George.Adams <@t> UTSouthwestern.edu (George Adams) Date: Tue Mar 13 15:26:37 2007 Subject: [Histonet] sorry for the confusion re: recommendation for kits Message-ID: <45F6C2A002000018000137F9@swnw126.swmed.edu> Histonetters: "abc" after the word chondroitinase refers to the specific isoform of chondroitinase, not avidin-biotin complex. Hoping not to ever send out another confusing e-mail in this lifetime, George Adams From liz <@t> premierlab.com Tue Mar 13 16:21:08 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Tue Mar 13 16:08:27 2007 Subject: [Histonet] cytoplasmic tunel staining Message-ID: <000001c765b5$84151a90$0d00a8c0@domain.Premier> Hello Everyone I just did a quick search on tunel and cytoplasmic staining and I did see a few references that say that this happens, but I also decided to check with the experts out there in histoland. I always thought that tunel staining was localized to the nuclei, but we just ran the roche kit on some human tonsil and we do have some nuclear staining, but I would have to say that the majority of staining that we are seeing is cytoplasmic and upon brief review the staining is consistent with the staining pattern that we get when we stain with cleaved caspase 3. Can this be correct? We do not perform tunel that often and I know that there are lots of issues with the pretreatment of paraffin sections and I just want to make sure I'm not creating the staining due to some procedural issue. We did run the appropriate negative controls and they were negative and our positive control was fine also. Any advice would be helpful. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 From cindipqr <@t> wowway.com Tue Mar 13 16:17:26 2007 From: cindipqr <@t> wowway.com (cindipqr@wowway.com) Date: Tue Mar 13 16:17:37 2007 Subject: [Histonet] mouse on mouse kit In-Reply-To: References: Message-ID: <20070313211541.M45233@wowway.com> Hi Helen, I've used Dako's Ark kit. It gives good results and is very easy to use. Cindi Roberts HFH Detroit, Mi ---------- Original Message ----------- From: Helen E Johnson To: histonet@lists.utsouthwestern.edu Sent: Tue, 13 Mar 2007 09:08:55 -0400 Subject: [Histonet] mouse on mouse kit > Hi Histonetters, > I need a recommendation on a IHC mouse on mouse detection kit. > Helen Johnson > (hej01@health.state.ny.us) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------- End of Original Message ------- From wayneozanne <@t> sympatico.ca Tue Mar 13 17:13:56 2007 From: wayneozanne <@t> sympatico.ca (W. Ozanne) Date: Tue Mar 13 17:14:09 2007 Subject: [Histonet] A Good Bile Control for Fouchet's Procedure Message-ID: To the experts, I currently have no good positive control for the Fouchet's SOP for detecting bile. We are currently using old, pre-cut positive control slides that I am sure at some point demonstrated a strong positive staining reaction. We are currently seeing positive green staining, however it is very light. I am certain this is from the old, precut slides. Any recommendations? Has anyone stained gall bladder and is it positive? Thanks for your ideas and suggestions. Wayne Ozanne, Chief Technologist & Laboratory Safety Officer From wayneozanne <@t> sympatico.ca Tue Mar 13 17:22:06 2007 From: wayneozanne <@t> sympatico.ca (W. Ozanne) Date: Tue Mar 13 17:22:12 2007 Subject: [Histonet] Cytology Cell Blocks and Poor Microtomy Due to Friction/Static Message-ID: Question for the Group, The group cutting cytology cell blocks are continually challenged with microtomy on a regular basis. There is so much static and friction from the cell blocks sections as the come off of the block onto the knife. Getting a section from the knife to the waterbath is no easy task. During the winter months we have a humidifier going in the cutting area and this does not seem to make a difference as this continues to be a challenge during the most humid of months of the year. Soaking the blocks after coarse trimming works to some degree, but you are lucky if you get one to two sections......ribbons would be considered a gift. The cell blocks are processed on the Leica with the routine blocks for the day according to a standard processing schedule. Has anyone else faced this challenge and how did you beat it????? From tahseen <@t> brain.net.pk Tue Mar 13 18:07:03 2007 From: tahseen <@t> brain.net.pk (Tahseen) Date: Tue Mar 13 18:13:02 2007 Subject: [Histonet] Acrylic resin Message-ID: <014501c765c4$ca52fc20$101480cb@piii> Dear All, My Pathologist want to know regarding Growth of organism, porosity & cracks in 2x2 cm specimens material of acrylic resin(PMMA),(pre-Inoculate). I have no idea, help please. Thanks advance. Muhammad Tahseen Histology Supervisor, Shaukat Khanum Memorial Cancer Hospital & Research Centre Lahore,Pakistan. tahseen@brain.net.pk From daydawning <@t> wideopenwest.com Wed Mar 14 06:06:19 2007 From: daydawning <@t> wideopenwest.com (daydawning@wideopenwest.com) Date: Wed Mar 14 06:06:27 2007 Subject: [Histonet] Re: Histonet Digest, Vol 40, Issue 16 In-Reply-To: <200703131632.l2DGWId15424@smtp-2.wideopenwest.com> References: <200703131632.l2DGWId15424@smtp-2.wideopenwest.com> Message-ID: <20070314105942.M15221@wideopenwest.com> I think I may have visited a nearly perfect histology lab today in South Bend, IN Specimens tubed from other off-site facilities (under the river, for heaven's sake!)no fumes, filing system for specimens,separate processing room, every step that can be automated is, and EVERY CUTTER HAS A WINDOW! Dawn Truscott Personal Email Message: 2 Date: Mon, 12 Mar 2007 16:26:51 -0400 From: "Marcia Funk" Subject: [Histonet] Histology Lab To: histonet@lists.utsouthwestern.edu Message-ID: <45F5713B020000AC0000044A@nodcmsngwia1.trinity-health.org> Content-Type: text/plain; charset=us-ascii Hello, If there was a perfect Histology Lab what would it be like ? Does anyone know if there are plans published for the perfect Histology lab. thanks From sonya.martin <@t> soton.ac.uk Wed Mar 14 07:05:42 2007 From: sonya.martin <@t> soton.ac.uk (Martin S.) Date: Wed Mar 14 07:07:27 2007 Subject: [Histonet] Problems with IHC/DAB on tumours Message-ID: <71437982F5B13A4D9A5B2669BDB89EE4023E3532@ISS-CL-EX-V1.soton.ac.uk> Hi All, I'm having a problem doing IHC/DAB on frozen colorectal tumour samples. I'm getting a lot of positive cells in sections even without without any antibodies or ABC. I've narrowed it down to the DAB but I dont think its DAB ppt as its not random - you can see the cells rapidly stain under the microscope after adding the DAB - it only takes a matter of seconds! I've been using DAB from Sigma and also Vector - both show the same result (I have filtered both with 0.2um filter). I do not see it in mouse liver or spleen tissue (frozen in OCT in a bath of isopentane on dry ice - the CR tumour is human and was snap frozen in liquid nitrogen). I dont think its endog peroxidase as it occurs even if you add the DAB diluted in peroxidase suppressor (Pierce - has always worked well for other tissues). Any suggestions? Is there something else that could be in the tissue that the DAB would react with? Would using AEC instead help? The Pierce suppressor is supposedly better than H2O2 methods - can anyone recommend other peroxidase inhibition protocols? Thanks Sonya From rjbuesa <@t> yahoo.com Wed Mar 14 07:49:42 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 14 07:49:50 2007 Subject: [Histonet] Cytology Cell Blocks and Poor Microtomy Due to Friction/Static In-Reply-To: Message-ID: <334237.19348.qm@web61216.mail.yahoo.com> IF (emphasis on the IF) it is static electricity the cause, I used to ground my microtome with a wire to a close water pipe (or you could lead the ground to the floor). Ren? J. "W. Ozanne" wrote: Question for the Group, The group cutting cytology cell blocks are continually challenged with microtomy on a regular basis. There is so much static and friction from the cell blocks sections as the come off of the block onto the knife. Getting a section from the knife to the waterbath is no easy task. During the winter months we have a humidifier going in the cutting area and this does not seem to make a difference as this continues to be a challenge during the most humid of months of the year. Soaking the blocks after coarse trimming works to some degree, but you are lucky if you get one to two sections......ribbons would be considered a gift. The cell blocks are processed on the Leica with the routine blocks for the day according to a standard processing schedule. Has anyone else faced this challenge and how did you beat it????? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Don't be flakey. Get Yahoo! Mail for Mobile and always stay connected to friends. From LSebree <@t> uwhealth.org Wed Mar 14 07:53:04 2007 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Wed Mar 14 07:53:13 2007 Subject: [Histonet] Cytology Cell Blocks and Poor Microtomy Due toFriction/Static In-Reply-To: <334237.19348.qm@web61216.mail.yahoo.com> Message-ID: I have been swabbing down my microtome and hands with "Static Guard"; really works well. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, March 14, 2007 7:50 AM To: W. Ozanne; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cytology Cell Blocks and Poor Microtomy Due toFriction/Static IF (emphasis on the IF) it is static electricity the cause, I used to ground my microtome with a wire to a close water pipe (or you could lead the ground to the floor). Ren? J. "W. Ozanne" wrote: Question for the Group, The group cutting cytology cell blocks are continually challenged with microtomy on a regular basis. There is so much static and friction from the cell blocks sections as the come off of the block onto the knife. Getting a section from the knife to the waterbath is no easy task. During the winter months we have a humidifier going in the cutting area and this does not seem to make a difference as this continues to be a challenge during the most humid of months of the year. Soaking the blocks after coarse trimming works to some degree, but you are lucky if you get one to two sections......ribbons would be considered a gift. The cell blocks are processed on the Leica with the routine blocks for the day according to a standard processing schedule. Has anyone else faced this challenge and how did you beat it????? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Don't be flakey. Get Yahoo! Mail for Mobile and always stay connected to friends. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Wed Mar 14 09:24:42 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Mar 14 09:25:12 2007 Subject: [Histonet] Re: Histonet Digest, Vol 40, Issue 16 In-Reply-To: <20070314105942.M15221@wideopenwest.com> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D3764@sjhaexc02.sjha.org> Are you sure you weren't in Histotech Heaven? That's amazing! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of daydawning@wideopenwest.com Sent: Wednesday, March 14, 2007 7:06 AM To: histonet@lists.utsouthwestern.edu Cc: histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 40, Issue 16 I think I may have visited a nearly perfect histology lab today in South Bend, IN Specimens tubed from other off-site facilities (under the river, for heaven's sake!)no fumes, filing system for specimens,separate processing room, every step that can be automated is, and EVERY CUTTER HAS A WINDOW! Dawn Truscott Personal Email Message: 2 Date: Mon, 12 Mar 2007 16:26:51 -0400 From: "Marcia Funk" Subject: [Histonet] Histology Lab To: histonet@lists.utsouthwestern.edu Message-ID: <45F5713B020000AC0000044A@nodcmsngwia1.trinity-health.org> Content-Type: text/plain; charset=us-ascii Hello, If there was a perfect Histology Lab what would it be like ? Does anyone know if there are plans published for the perfect Histology lab. thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From PMonfils <@t> Lifespan.org Wed Mar 14 10:19:55 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Mar 14 10:20:09 2007 Subject: [Histonet] Cytology Cell Blocks and Poor Microtomy Due toFriction/Static In-Reply-To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E273C56@LSRIEXCH1.lsmaster.lifespan.org> Grounding the microtome with a wire can help. Also, a Staticmaster brush, if it is still available, is very helpful. You just brush around the knife holder and the static is neutralized. I have one I bought years ago. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of W. Ozanne > Sent: Tuesday, March 13, 2007 6:22 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Cytology Cell Blocks and Poor Microtomy Due toFriction/Static > > Question for the Group, > > The group cutting cytology cell blocks are continually challenged with > microtomy on a regular basis. There is so much static and friction from the > cell blocks sections as the come off of the block onto the knife. Getting a > section from the knife to the waterbath is no easy task. > During the winter months we have a humidifier going in the cutting area and > this does not seem to make a difference as this continues to be a challenge > during the most humid of months of the year. > Soaking the blocks after coarse trimming works to some degree, but you are > lucky if you get one to two sections......ribbons would be considered a > gift. > The cell blocks are processed on the Leica with the routine blocks for the > day according to a standard processing schedule. > > Has anyone else faced this challenge and how did you beat it????? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From jbirkner <@t> colabserv.com Wed Mar 14 11:07:16 2007 From: jbirkner <@t> colabserv.com (Jeff Birkner) Date: Wed Mar 14 11:07:26 2007 Subject: [Histonet] her2 asco/cap Message-ID: Dear Histonetters: We are just starting to do her2 testing in house. Does anyone have a procedure they would be willing to share especially dealing with the new asco/cap guidelines? Any help would be greatly appreciated. Thanks! Jeff Birkner From LGaliotto <@t> nch.org Wed Mar 14 11:46:27 2007 From: LGaliotto <@t> nch.org (Galiotto, Laura) Date: Wed Mar 14 11:46:39 2007 Subject: [Histonet] Meconium lining Message-ID: <270614B321ACB44D8C1D91F4F921FDC304B16397@NCH01EX02.nch.org> Hello Fellow Histoneters Can anyone share their in-put or experiences with the presence or lack of meconium lining on H&E? thank you Laura ******************* PLEASE NOTE ******************* This E-Mail/telefax message and any documents accompanying this transmission may contain information that is privileged, confidential, and/or exempt from disclosure under applicable law and is intended solely for the addressee(s) named above. If you are not the intended addressee/recipient, you are hereby notified that any use of, disclosure, copying, distribution, or reliance on the contents of this E-Mail/telefax information is strictly prohibited and may result in legal action against you. Please reply to the sender advising of the error in transmission and immediately delete/destroy the message and any accompanying documents. Thank you. From alaskagirl1950 <@t> yahoo.com Wed Mar 14 13:16:07 2007 From: alaskagirl1950 <@t> yahoo.com (Patricia Adams) Date: Wed Mar 14 13:16:17 2007 Subject: [Histonet] Mailing containers Message-ID: <714716.74706.qm@web52508.mail.yahoo.com> Histonet world, I am looking for a place to order mailers. The type I am looking for is the white screw top container with the business reply label printed on it. And a prefilled formalin container inside. If anyone can tell me where to look I would be grateful. So far I have checked every catalog I have and no luck. Thank you, Patricia Adams, HT (ASCP) Tuskegee University Tuskegee, Alabama Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D ____________________________________________________________________________________ Expecting? Get great news right away with email Auto-Check. Try the Yahoo! Mail Beta. http://advision.webevents.yahoo.com/mailbeta/newmail_tools.html From TMcNemar <@t> lmhealth.org Wed Mar 14 13:24:58 2007 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Wed Mar 14 13:24:51 2007 Subject: [Histonet] Alizarin Red S for gunshot residue Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F46A@lmhsmail.lmhealth.org> I've been asked about doing an alizarin red s stain for gunshot residue by the folks at our local coroner's office. I find many references to it but no specific procedure for it. Does anyone do this or know of a procedure for it? Would something like Dahl's work for gunshot? Appreciate any help. Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org From CIngles <@t> uwhealth.org Wed Mar 14 13:33:31 2007 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Wed Mar 14 13:33:41 2007 Subject: [Histonet] Histology Lab References: <5.2.0.9.0.20070312153905.02b94420@pop.central.cox.net> Message-ID: <08A0A863637F1349BBFD83A96B27A50A120006@uwhis-xchng3.uwhis.hosp.wisc.edu> ________________________________ frankly, the floor should be durable/impervious to chemicals (and the clumsy tech). i'm thinking sealed concrete with a drain in the middle. think kill-floor at the slaughterhouse. fitting. no? ;) deb Hey, I used to work at a meat packing plant. Good thing I wasn't on the kill floor. Hmmm.. Is that why I like Histology and Grossing doesn't "gross" me out? At least histology pays better, you have to wait a little longer to get carpel tunnel, and don't have to work in a 40 degree refrigerated area all day. Don't forget to have some form of textured surface on top of that. Basic concrete would kill your feet, and you would be skating on paraffin. Claire From rsrichmond <@t> aol.com Wed Mar 14 13:37:20 2007 From: rsrichmond <@t> aol.com (rsrichmond@aol.com) Date: Wed Mar 14 13:37:44 2007 Subject: [Histonet] Re: A Good Bile Control For Fouchet's Procedure In-Reply-To: <200703141404.1b445f839232bd@rly-yc01.mail.aol.com> References: <200703141404.1b445f839232bd@rly-yc01.mail.aol.com> Message-ID: <8C9347EEEF8C9A0-1428-690D@mblk-d44.sysops.aol.com> Wayne Ozanne (where in Canada?) writes: >>I currently have no good positive control for the Fouchet's SOP for detecting bile. We are currently using old, pre-cut positive control slides that I am sure at some point demonstrated a strong positive staining reaction. We are currently seeing positive green staining, however it is very light. I am certain this is from the old, precut slides. - Has anyone stained gall bladder and is it positive?<< I've never known anyone to actually do a bile stain, because bile is usually obvious in the H & E. Oxidizing agents oxidize yellow-brown bilirubin to green biliverdin. Hall's technique (1960) uses Fouchet's (pronounced foo-SHAY) reagent (1917), a fresh mixture of trichloracetic acid and ferric chloride, as the oxidizing agent. (All this from Bancroft & Stevens). Lee Luna in the AFIP manual has a complex method for meconium in placentas that may be worth looking at. The issue is rarely addressed, but I think that meconium has the same staining reactions as ordinary bilirubin, so that you might be able to use a block of rolled fetal membranes with meconium macrophages (very common) as a control. (If you try this, please let us all know the results.) An occasional section of gallbladder will have some stainable bile, but I'd prefer liver - probably from an autopsy - with bile stasis. Could you locate such a case in your files? Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From lblazek <@t> digestivespecialists.com Wed Mar 14 13:52:43 2007 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Mar 14 13:48:53 2007 Subject: [Histonet] Alizarin Red S for gunshot residue References: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F46A@lmhsmail.lmhealth.org> Message-ID: <6CBA6DC98A079D408C87250591D9DFB802684D9A@bruexchange.digestivespecialists.com> Tom, There is a procedure in Sheehan's Theory and Practice of Histotechnology if that helps any. - Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Wednesday, March 14, 2007 2:25 PM To: Histonet (E-mail) Subject: [Histonet] Alizarin Red S for gunshot residue I've been asked about doing an alizarin red s stain for gunshot residue by the folks at our local coroner's office. I find many references to it but no specific procedure for it. Does anyone do this or know of a procedure for it? Would something like Dahl's work for gunshot? Appreciate any help. Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Wed Mar 14 13:50:27 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed Mar 14 13:50:45 2007 Subject: Dryer sheets for RE: [Histonet] Cytology Cell Blocks and Poor Microtomy Due toFriction/Static In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E273C56@LSRIEXCH1.lsmaster. lifespan.org> References: <4EBFF65383B74D49995298C4976D1D5E273C56@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <6.0.0.22.1.20070314124618.01b40fa0@gemini.msu.montana.edu> Someone has not mentioned rubbing micrtome parts with anti-static, preferably unscented dryer sheets from home. One can also humdify the blade holder and block face by wiping with a cold water dampened guaze. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From Jessica.Vacca <@t> HCAhealthcare.com Wed Mar 14 14:04:42 2007 From: Jessica.Vacca <@t> HCAhealthcare.com (Vacca Jessica) Date: Wed Mar 14 14:04:54 2007 Subject: [Histonet] Meditech Message-ID: <41E16A15CE78374EA45B57E0F94339B8021F37F7@ORLEV01.hca.corpad.net> I was wondering if there were a bunch of pathology users of meditech that would like to start a user support group. I'm not sure if it is the same everywhere as far as the many applications that meditech has, but I'm trying to start one within my HCA facility (that includes all HCA not just my division) and I'm getting no where and have simple questions that I know others are doing just not quite sure how to get there..........anyone interested send me an email and I'll have my IS guy help me create a group like this that we can ask each other questions. (if it is possible) Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Dr. Brandon Fl 33511 (813) 571-5193 or (813) 681-5551 ext 2454 Jessica.Vacca@hcahealthcare.com From fayzullina <@t> anesiva.com Wed Mar 14 15:18:46 2007 From: fayzullina <@t> anesiva.com (Saniya Fayzullina) Date: Wed Mar 14 15:18:56 2007 Subject: [Histonet] histology of rat knee joint for TRPV1, CGRP, SP Message-ID: Hello, I am trying to work out a protocol for immunohistochemistry on the rat knee. I have the following questions: 1. Is decalcification necessary? How long is enough in EDTA? I will probably embed in OCT, and cut on a cryostat, so I am assuming the bones might create a problem. 2. Do 20um sections sound reasonable for the joint? 3. I would prefer to stick with a simple block->primary->fluorophore-conjugated-secondary protocol, rather than ABC, and dealing with streptavidin etc steps. Has anyone successfully used a TRPV1 receptor antibody on rat tissue? CGRP? SP? 4. What do you think about the formalin-picric acid perfusion method? Does the picric acid really make a big difference? I have seen it used at 0.2%, 2%, and 15%. Which is preferred? Thanks! ~Saniya From Luis.Chiriboga <@t> med.nyu.edu Wed Mar 14 17:31:53 2007 From: Luis.Chiriboga <@t> med.nyu.edu (Luis Chiriboga) Date: Wed Mar 14 16:26:31 2007 Subject: [Histonet] Ventana Benchmark Reagents Message-ID: Hi Everyone For those of you doing ISH on benchmark, have any of you noticed a change in the expiration dates of CC1? Ours went from a 1year exp date down to 2 months???? anybody have a similar experience? Thanks Luis ____________________________________ Luis Chiriboga Ph.D. NYU Cancer Institute and Bellevue Hospital Center New York University School Of Medicine Department Of Pathology 4W27 462 First Avenue New York, N.Y. 10016 W(212) 562-4667. F(212) 263-2041 From zumbor <@t> email.cs.nsw.gov.au Wed Mar 14 17:10:53 2007 From: zumbor <@t> email.cs.nsw.gov.au (Rosalba) Date: Wed Mar 14 17:15:12 2007 Subject: [Histonet] A Good Bile Control for Fouchet's Procedure Message-ID: <01C766E1.D5BC2320.zumbor@email.cs.nsw.gov.au> The control slides for Fouchet should be cut only when you need them they deteriorate once cut. The stain should turn the bile a bluish green as opposed to the intrinsic yellowish green of the bile. Rosalba Zumbo Supervisor Histology Dept Department of Forensic Medicine 42-50 Parramatta Rd Glebe NSW 2037 Australia PH: 61 2 85847842 FAX: 61 2 95664573 zumbor@email.cs.nsw.gov.au -----Original Message----- From: W. Ozanne [SMTP:wayneozanne@sympatico.ca] Sent: Wednesday, 14 March 2007 9:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] A Good Bile Control for Fouchet's Procedure To the experts, I currently have no good positive control for the Fouchet's SOP for detecting bile. We are currently using old, pre-cut positive control slides that I am sure at some point demonstrated a strong positive staining reaction. We are currently seeing positive green staining, however it is very light. I am certain this is from the old, precut slides. Any recommendations? Has anyone stained gall bladder and is it positive? Thanks for your ideas and suggestions. Wayne Ozanne, Chief Technologist & Laboratory Safety Officer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____________________________________________________________________ This email has been scanned for the Sydney South West Area Health Service by the MessageLabs Email Security System. SSWAHS regularly monitors emails and attachments to ensure compliance with the NSW Government's Electronic Messaging Policy. "This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please destroy it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of the Sydney South West Area Health Service." From sjchtascp <@t> yahoo.com Wed Mar 14 17:58:15 2007 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Wed Mar 14 17:58:24 2007 Subject: [Histonet] slide drying Message-ID: <616372.42485.qm@web38212.mail.mud.yahoo.com> Has anyone ever used a 60C- 70C incubator to dry histology slides? Would the non-circulation of heated air cause inadequate drying of slides??? --------------------------------- TV dinner still cooling? Check out "Tonight's Picks" on Yahoo! TV. From sjchtascp <@t> yahoo.com Wed Mar 14 18:23:33 2007 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Wed Mar 14 18:23:41 2007 Subject: [Histonet] Re: perfect histology lab In-Reply-To: <308257.75784.qm@web53609.mail.yahoo.com> Message-ID: <930081.69339.qm@web38205.mail.mud.yahoo.com> LOL, perfect histology lab ...ya right. How about a snot nose newbie tech right out of school, no clinical experience at all, running the show, spouting her theory and superior knowledge and intellect, brown nosing the boss that's now in her back pocket. All the experienced techs 14-40 years experience each moving right out the door. Go figure. And the boss can't understand why she can't keep techs. --------------------------------- Now that's room service! Choose from over 150,000 hotels in 45,000 destinations on Yahoo! Travel to find your fit. From proctoth <@t> ohsu.edu Wed Mar 14 18:45:09 2007 From: proctoth <@t> ohsu.edu (Thomas Proctor) Date: Wed Mar 14 18:46:03 2007 Subject: [Histonet] Re: perfect histology lab Message-ID: I guess I am lucky; I run a histology core for an exclusive group of 6 scientists who are all studying neuroimmunology--and I'm the only person in my histology lab! The only thing expected of me is results, no drama to speak of. It's all my stuff, my mess. Perfect? I don't know, but it sure is nice. >>> "Steven Coakley" 03/14/07 4:23 PM >>> LOL, perfect histology lab ...ya right. How about a snot nose newbie tech right out of school, no clinical experience at all, running the show, spouting her theory and superior knowledge and intellect, brown nosing the boss that's now in her back pocket. All the experienced techs 14-40 years experience each moving right out the door. Go figure. And the boss can't understand why she can't keep techs. --------------------------------- Now that's room service! Choose from over 150,000 hotels in 45,000 destinations on Yahoo! Travel to find your fit. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sharmila.chapagain <@t> jcu.edu.au Thu Mar 15 01:41:36 2007 From: sharmila.chapagain <@t> jcu.edu.au (Sharmila Chapagain) Date: Thu Mar 15 01:41:46 2007 Subject: [Histonet] Remove from email list Message-ID: <20070315164136.BIO17252@mirapoint-ms1.jcu.edu.au> Dear All I would like to request you please remove my list from histonet email. Thank you. sharmila From Marilyn.Tyler <@t> uct.ac.za Thu Mar 15 02:23:00 2007 From: Marilyn.Tyler <@t> uct.ac.za (Marilyn Tyler) Date: Thu Mar 15 02:23:18 2007 Subject: [Histonet] Eosinophils Message-ID: <45F91073.A78E.0090.0@uct.ac.za> Morning All Anyone have a stain for eosinophils in FFP mouse lung tissue and also nerve stain in FFP mouse intestine for a technologist in our research lab. Thanks Marilyn From BMolinari <@t> heart.thi.tmc.edu Thu Mar 15 05:29:55 2007 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Thu Mar 15 05:30:01 2007 Subject: [Histonet] Re: perfect histology lab In-Reply-To: Message-ID: I don't have to guess, I know I am lucky. I too am the lone histotech and do paraffin, frozens and plastics. I have worked with my supervisor for 17 years and you couldn't ask for a better person. I am left to my own devices as long as the results are good and on time. I can pretty much design my own schedule and yes, I have windows. Nirvana. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Proctor Sent: Wednesday, March 14, 2007 6:45 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: perfect histology lab I guess I am lucky; I run a histology core for an exclusive group of 6 scientists who are all studying neuroimmunology--and I'm the only person in my histology lab! The only thing expected of me is results, no drama to speak of. It's all my stuff, my mess. Perfect? I don't know, but it sure is nice. >>> "Steven Coakley" 03/14/07 4:23 PM >>> LOL, perfect histology lab ...ya right. How about a snot nose newbie tech right out of school, no clinical experience at all, running the show, spouting her theory and superior knowledge and intellect, brown nosing the boss that's now in her back pocket. All the experienced techs 14-40 years experience each moving right out the door. Go figure. And the boss can't understand why she can't keep techs. --------------------------------- Now that's room service! Choose from over 150,000 hotels in 45,000 destinations on Yahoo! Travel to find your fit. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dlcowie <@t> prodigy.net Thu Mar 15 05:37:58 2007 From: dlcowie <@t> prodigy.net (Dawn Cowie) Date: Thu Mar 15 05:38:09 2007 Subject: [Histonet] Ventana Benchmark Reagents In-Reply-To: Message-ID: <250303.21590.qm@web81004.mail.mud.yahoo.com> Is this a 1 time occurence or have you noticed this on several shipments? Dawn Luis Chiriboga wrote: Hi Everyone For those of you doing ISH on benchmark, have any of you noticed a change in the expiration dates of CC1? Ours went from a 1year exp date down to 2 months???? anybody have a similar experience? Thanks Luis ____________________________________ Luis Chiriboga Ph.D. NYU Cancer Institute and Bellevue Hospital Center New York University School Of Medicine Department Of Pathology 4W27 462 First Avenue New York, N.Y. 10016 W(212) 562-4667. F(212) 263-2041 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Luis.Chiriboga <@t> med.nyu.edu Thu Mar 15 09:16:02 2007 From: Luis.Chiriboga <@t> med.nyu.edu (Luis Chiriboga) Date: Thu Mar 15 08:09:46 2007 Subject: [Histonet] Ventana Benchmark Reagents In-Reply-To: <250303.21590.qm@web81004.mail.mud.yahoo.com> Message-ID: originally the expiration interval was a year. the next shipment was down to 6 months. contacted ventana and they say that they make "no guarantee on the life of the reagent"??? if you look at the msds, there's nothing in there that would expire anyway, at least not reported at any significant concentration. BTW the reagent was cc2 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Dawn Cowie Sent: Thursday, March 15, 2007 5:38 AM To: Luis Chiriboga; histonet Subject: Re: [Histonet] Ventana Benchmark Reagents Is this a 1 time occurence or have you noticed this on several shipments? Dawn Luis Chiriboga wrote: Hi Everyone For those of you doing ISH on benchmark, have any of you noticed a change in the expiration dates of CC1? Ours went from a 1year exp date down to 2 months???? anybody have a similar experience? Thanks Luis ____________________________________ Luis Chiriboga Ph.D. NYU Cancer Institute and Bellevue Hospital Center New York University School Of Medicine Department Of Pathology 4W27 462 First Avenue New York, N.Y. 10016 W(212) 562-4667. F(212) 263-2041 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Robinsoc <@t> mercyhealth.com Thu Mar 15 08:33:28 2007 From: Robinsoc <@t> mercyhealth.com (Cindy Robinson) Date: Thu Mar 15 08:33:59 2007 Subject: [Histonet] Ventana Benchmark Reagents In-Reply-To: References: Message-ID: <45F904D80200000F001D9F86@nodcmsngwia1.trinity-health.org> The expiration on CC2 is 3 months at the longest in my experience. I received some reagents which were outside the guaranteed outdate lengths....NeuVis nuclear stain which is supposed to have a minimum of six months use after receipt date and I had from 4 weeks to 3 months. I called Ventana and got credit for these reagents. Ventana can give you a list with the guaranteed lengths of outdate from the receive date. Cindi >>> "Luis Chiriboga" 3/15/2007 9:16 AM >>> originally the expiration interval was a year. the next shipment was down to 6 months. contacted ventana and they say that they make "no guarantee on the life of the reagent"??? if you look at the msds, there's nothing in there that would expire anyway, at least not reported at any significant concentration. BTW the reagent was cc2 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Dawn Cowie Sent: Thursday, March 15, 2007 5:38 AM To: Luis Chiriboga; histonet Subject: Re: [Histonet] Ventana Benchmark Reagents Is this a 1 time occurence or have you noticed this on several shipments? Dawn Luis Chiriboga wrote: Hi Everyone For those of you doing ISH on benchmark, have any of you noticed a change in the expiration dates of CC1? Ours went from a 1year exp date down to 2 months???? anybody have a similar experience? Thanks Luis ____________________________________ Luis Chiriboga Ph.D. NYU Cancer Institute and Bellevue Hospital Center New York University School Of Medicine Department Of Pathology 4W27 462 First Avenue New York, N.Y. 10016 W(212) 562-4667. F(212) 263-2041 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NHeath <@t> Lifespan.org Thu Mar 15 09:02:58 2007 From: NHeath <@t> Lifespan.org (Heath, Nancy L.) Date: Thu Mar 15 09:03:09 2007 Subject: [Histonet] Does anyone know where Jean Mitchell is ??? Message-ID: <130E8991F210424096EFC6F42EA33B2422405B@LSCOEXCH1.lsmaster.lifespan.org> Hi, Does anyone out in Histoland know where Jean Mitchell from the Wisconsin Neuropathology Lab is???" Thanks, Nancy Heath Rhode Island Hospital nheath@lifespan.org From algranth <@t> u.arizona.edu Thu Mar 15 09:17:48 2007 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Thu Mar 15 09:17:58 2007 Subject: [Histonet] Re: perfect histology lab In-Reply-To: References: Message-ID: <4.3.2.7.2.20070315070941.00dc9d18@algranth.inbox.email.arizona.edu> Ditto Betsy! Except that I don't do plastics, have been with my supervisor only going on 8 years but I work 4 ten hr days/week so have long weekends EVERY week! My instruments are new (1-5yrs) and I have inherited glassware and chemicals and stains to do just about any stain I want to do...but the windows...I love the windows! Andi At 05:29 AM 3/15/2007 -0500, Molinari, Betsy wrote: >I don't have to guess, I know I am lucky. I too am the lone histotech >and do paraffin, frozens and plastics. I have worked with my supervisor >for 17 years and you couldn't ask for a better person. I am left to my >own devices as long as the results are good and on time. I can pretty >much design my own schedule and yes, I have windows. Nirvana. > >Betsy Molinari HT (ASCP) >Texas Heart Institute >Cardiovascular Pathology >6770 Bertner Ave. >Houston,TX 77030 >832-355-6524 >832-355-6812 (fax) > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas >Proctor >Sent: Wednesday, March 14, 2007 6:45 PM >To: histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] Re: perfect histology lab > >I guess I am lucky; I run a histology core for an exclusive group of 6 >scientists who are all studying neuroimmunology--and I'm the only person >in my histology lab! The only thing expected of me is results, no drama >to speak of. It's all my stuff, my mess. Perfect? I don't know, but >it sure is nice. > > >>> "Steven Coakley" 03/14/07 4:23 PM >>> >LOL, perfect histology lab ...ya right. How about a snot nose newbie >tech right out of school, no clinical experience at all, running the >show, spouting her theory and superior knowledge and intellect, brown >nosing the boss that's now in her back pocket. All the experienced >techs 14-40 years experience each moving right out the door. Go figure. >And the boss can't understand why she can't keep techs. > > > > > > >--------------------------------- >Now that's room service! Choose from over 150,000 hotels >in 45,000 destinations on Yahoo! Travel to find your fit. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From gcallis <@t> montana.edu Thu Mar 15 09:48:17 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Mar 15 09:48:32 2007 Subject: [Histonet] slide drying In-Reply-To: <616372.42485.qm@web38212.mail.mud.yahoo.com> References: <616372.42485.qm@web38212.mail.mud.yahoo.com> Message-ID: <6.0.0.22.1.20070315084220.01b366c0@gemini.msu.montana.edu> Yes - for the last 27 years, but never at 70C. We have a dry air incubator set at 60C, just enough to melt the paraffin on sections. This incubator is also used to melt paraffin in a metal beaker in case we need to top off our processor paraffins. Drying time is usually 45 min or so for sections on well drained Plus charge slides, and drying of slides has been excellent. At 04:58 PM 3/14/2007, you wrote: >Has anyone ever used a 60C- 70C incubator to dry histology slides? Would >the non-circulation of heated air cause inadequate drying of slides??? > >--------------------------------- >TV dinner still cooling? >Check out "Tonight's Picks" on Yahoo! TV. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From gcallis <@t> montana.edu Thu Mar 15 09:58:38 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Mar 15 09:58:52 2007 Subject: [Histonet] Eosinophils In-Reply-To: <45F91073.A78E.0090.0@uct.ac.za> References: <45F91073.A78E.0090.0@uct.ac.za> Message-ID: <6.0.0.22.1.20070315085057.01b62270@gemini.msu.montana.edu> We use an H&E (Richard Allan hematoxylin 1 and eosin Y) in order to count eosinophils in lungs. Our sections are cut at 2 - 3 um to avoid so much cellular overlap, then do a standard H&E stain with longer time in the eosin - 2 minutes instead of 1. This time can be adjusted for your lab needs. Nothing else has worked as well for us. The biggest problem has been people not very well versed in eosinophil morphology in an H&E stained section. At 01:23 AM 3/15/2007, you wrote: >Morning All >Anyone have a stain for eosinophils in FFP mouse lung tissue and also >nerve stain in FFP mouse intestine for a technologist in our research >lab. Thanks >Marilyn > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From MSHERWOOD <@t> PARTNERS.ORG Thu Mar 15 09:56:58 2007 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Thu Mar 15 10:03:56 2007 Subject: [Histonet] Re: Cox-2 Monoclonal Antibody Message-ID: <1AF23D0AD12E7444A5DB083CA978B73407A3101E@PHSXMB1.partners.org> To all: I will be running immunohistochemistry on biopsies of human esophagus epithelium: squamous (wh/ should be normal) and columnar mucosa (wh/ should be a pre-cancerous condition known as Barrett's Esophagus). Question(s): 1) what would be good (+) controls for this antibody (Cox-2 Monoclonal)? 2) Any other tips you could share with me? Note: I will be doing this study manually. Thanks in advance! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-2106 (fax) msherwood@partners.org The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From rjbuesa <@t> yahoo.com Thu Mar 15 10:16:59 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Mar 15 10:17:08 2007 Subject: [Histonet] slide drying In-Reply-To: <616372.42485.qm@web38212.mail.mud.yahoo.com> Message-ID: <365338.45755.qm@web61216.mail.yahoo.com> I have used them with good results (many years ago!). Heat is heat. Ren? J. Steven Coakley wrote: Has anyone ever used a 60C- 70C incubator to dry histology slides? Would the non-circulation of heated air cause inadequate drying of slides??? --------------------------------- TV dinner still cooling? Check out "Tonight's Picks" on Yahoo! TV. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Bored stiff? Loosen up... Download and play hundreds of games for free on Yahoo! Games. From Jane.Parr <@t> UCHSC.edu Thu Mar 15 10:47:47 2007 From: Jane.Parr <@t> UCHSC.edu (Jane.Parr@UCHSC.edu) Date: Thu Mar 15 10:48:00 2007 Subject: [Histonet] Does anyone know where Jean Mitchell is ??? In-Reply-To: <130E8991F210424096EFC6F42EA33B2422405B@LSCOEXCH1.lsmaster.lifespan.org> Message-ID: <38ABC697651306429CC20F8F366CB26CCA3437@latte.uchsc.edu> Can't say I have had a "Jean Mitchell" sighting lately. Bet she is lurking somewhere. She emailed a few days back about the tri-state symposium. Jane -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heath, Nancy L. Sent: Thursday, March 15, 2007 8:03 AM To: Histonet (E-mail) Subject: [Histonet] Does anyone know where Jean Mitchell is ??? Hi, Does anyone out in Histoland know where Jean Mitchell from the Wisconsin Neuropathology Lab is???" Thanks, Nancy Heath Rhode Island Hospital nheath@lifespan.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From stern <@t> ipmc.cnrs.fr Thu Mar 15 11:28:31 2007 From: stern <@t> ipmc.cnrs.fr (Alejandro Ortiz Stern) Date: Thu Mar 15 11:20:16 2007 Subject: [Histonet] Qdots Message-ID: <45F9742F.6050905@ipmc.cnrs.fr> I would like to know if anyone has experience with Streptavidine-Qdots for the labeling of Acetone post-fixed slides (IHF). How do you wash the slides in order to get the best signal to noise ratio? Thank you for your input on the matter. -- Alejandro Ortiz Stern M.D. Ph.D., Postdoctoral Fellow Institut National de la Sant? et de la Recherche M?dicale, E0344 Universit? de Nice-Sophia-Antipolis Institut de Pharmacologie Mol?culaire et Cellulaire 660 Route des Lucioles - 06560 Valbonne - FRANCE TEL: 33 (4) 93 95 77 80 - FAX: 33 (4) 93 95 77 08 From JMacDonald <@t> mtsac.edu Thu Mar 15 11:21:52 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Mar 15 11:22:03 2007 Subject: [Histonet] Eosinophils In-Reply-To: <45F91073.A78E.0090.0@uct.ac.za> Message-ID: A Wright-Giemsa would work, so would a Diff-Quik. Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu "Marilyn Tyler" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/15/2007 12:23 AM To "histonet" cc Subject [Histonet] Eosinophils Morning All Anyone have a stain for eosinophils in FFP mouse lung tissue and also nerve stain in FFP mouse intestine for a technologist in our research lab. Thanks Marilyn _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcline <@t> wchsys.org Thu Mar 15 12:03:49 2007 From: jcline <@t> wchsys.org (Joyce Cline) Date: Thu Mar 15 12:04:05 2007 Subject: [Histonet] Ventana Benchmark Reagents In-Reply-To: Message-ID: <000001c76723$e7969430$1d2a14ac@wchsys.org> Our CC1 expires in July !!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Luis Chiriboga Sent: Wednesday, March 14, 2007 6:32 PM To: HISTONET Subject: [Histonet] Ventana Benchmark Reagents Hi Everyone For those of you doing ISH on benchmark, have any of you noticed a change in the expiration dates of CC1? Ours went from a 1year exp date down to 2 months???? anybody have a similar experience? Thanks Luis ____________________________________ Luis Chiriboga Ph.D. NYU Cancer Institute and Bellevue Hospital Center New York University School Of Medicine Department Of Pathology 4W27 462 First Avenue New York, N.Y. 10016 W(212) 562-4667. F(212) 263-2041 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From turkekul <@t> gmail.com Thu Mar 15 12:21:03 2007 From: turkekul <@t> gmail.com (mesruh turkekul) Date: Thu Mar 15 12:21:13 2007 Subject: [Histonet] anti static Message-ID: Hi Gayle, I have tried the anti-static bounty tissues but with no success. Now I am using static guard spray which works for me. which tissues papers do you use? Cheers, Mesruh Turkekul Molecular Biologist Memorial Sloan-Kettering Center Molecular Cytology New York, NY 10021 646-8882209\ turkekum@mskcc.org From melliott <@t> mrl.ubc.ca Thu Mar 15 12:27:50 2007 From: melliott <@t> mrl.ubc.ca (Mark Elliott) Date: Thu Mar 15 12:29:14 2007 Subject: [Histonet] Eosinophils Message-ID: <45F91FA6020000D6000185DF@mail.mrl.ubc.ca> We use Hansell's stain for demonstrating them in human and guinea pig lung. Think we have also done it in rabbit-should work in mice. Am not at work today but can send the method Friday. Mark >>> Jennifer MacDonald 03/15/07 9:21 AM >>> A Wright-Giemsa would work, so would a Diff-Quik. Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu "Marilyn Tyler" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/15/2007 12:23 AM To "histonet" cc Subject [Histonet] Eosinophils Morning All Anyone have a stain for eosinophils in FFP mouse lung tissue and also nerve stain in FFP mouse intestine for a technologist in our research lab. Thanks Marilyn _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***CONFIDENTIALITY NOTICE*** This electronic message is intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From turkekul <@t> gmail.com Thu Mar 15 12:35:26 2007 From: turkekul <@t> gmail.com (mesruh turkekul) Date: Thu Mar 15 12:35:34 2007 Subject: [Histonet] bone decalcification Message-ID: Hi Saniya, I fix the bones in 4% PFA in PBS at 4C for 48 hours. Then I place the bones in 0.5M EDTA (disodium salt) pH=8.0 and keep them for 10-20 days at 4C rocking and changing the EDTA every 3 days. Then I do 30% sucrose and OCT embedding. 20 micron is very thick try to cut 8-10 microns. Directly conjugated secondary is better and easier if it works! If it does not you may try the other options...... I think personally that picric acid is niot good idea to fix for IHC, unless the antibody does not work with regular fixatives and is reported to work with picric acid. Good luck!!!! Mesruh Turkekul Molecular Biologist Memorial Sloan-Kettering Center Molecular Cytology New York, NY 10021 646-8882209\ turkekum@mskcc.org From turkekul <@t> gmail.com Thu Mar 15 12:39:35 2007 From: turkekul <@t> gmail.com (mesruh turkekul) Date: Thu Mar 15 12:40:02 2007 Subject: [Histonet] q-dots Message-ID: Hi I have used the Str-Q-Dots before and after they were provided by Invitrogen. I did not see any signal both manually and automated with ventana machines. If you have results let me know your protocol... Cheers, Mesruh Turkekul Molecular Biologist Memorial Sloan-Kettering Center Molecular Cytology New York, NY 10021 646-8882209\ turkekum@mskcc.org From BMolinari <@t> heart.thi.tmc.edu Thu Mar 15 12:46:11 2007 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Thu Mar 15 12:46:19 2007 Subject: [Histonet] Re: perfect histology lab In-Reply-To: <4.3.2.7.2.20070315070941.00dc9d18@algranth.inbox.email.arizona.edu> Message-ID: Oh and I forgot to mention the best part! The pathologist is off site and only comes into the lab once or twice a month!!! Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: Andrea Grantham [mailto:algranth@u.arizona.edu] Sent: Thursday, March 15, 2007 9:18 AM To: Molinari, Betsy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: perfect histology lab Ditto Betsy! Except that I don't do plastics, have been with my supervisor only going on 8 years but I work 4 ten hr days/week so have long weekends EVERY week! My instruments are new (1-5yrs) and I have inherited glassware and chemicals and stains to do just about any stain I want to do...but the windows...I love the windows! Andi At 05:29 AM 3/15/2007 -0500, Molinari, Betsy wrote: >I don't have to guess, I know I am lucky. I too am the lone histotech >and do paraffin, frozens and plastics. I have worked with my supervisor >for 17 years and you couldn't ask for a better person. I am left to my >own devices as long as the results are good and on time. I can pretty >much design my own schedule and yes, I have windows. Nirvana. > >Betsy Molinari HT (ASCP) >Texas Heart Institute >Cardiovascular Pathology >6770 Bertner Ave. >Houston,TX 77030 >832-355-6524 >832-355-6812 (fax) > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas >Proctor >Sent: Wednesday, March 14, 2007 6:45 PM >To: histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] Re: perfect histology lab > >I guess I am lucky; I run a histology core for an exclusive group of 6 >scientists who are all studying neuroimmunology--and I'm the only person >in my histology lab! The only thing expected of me is results, no drama >to speak of. It's all my stuff, my mess. Perfect? I don't know, but >it sure is nice. > > >>> "Steven Coakley" 03/14/07 4:23 PM >>> >LOL, perfect histology lab ...ya right. How about a snot nose newbie >tech right out of school, no clinical experience at all, running the >show, spouting her theory and superior knowledge and intellect, brown >nosing the boss that's now in her back pocket. All the experienced >techs 14-40 years experience each moving right out the door. Go figure. >And the boss can't understand why she can't keep techs. > > > > > > >--------------------------------- >Now that's room service! Choose from over 150,000 hotels >in 45,000 destinations on Yahoo! Travel to find your fit. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From rjr6 <@t> psu.edu Thu Mar 15 12:53:41 2007 From: rjr6 <@t> psu.edu (Roberta Horner) Date: Thu Mar 15 12:53:57 2007 Subject: [Histonet] CBG Biotech solvent recycler Message-ID: Does anyone use the Biotech recycler for alcohols and xylene substitutes? Could you tell me what you think about them? I have been trying to talk the higher ups about getting a recycler but am tired of jumping through hoops. Roberta Horner HT HTL Penn State University Animal Diagnostic Lab From doug <@t> ppspath.com Thu Mar 15 14:03:13 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Thu Mar 15 13:01:52 2007 Subject: [Histonet] Re: perfect histology lab In-Reply-To: Message-ID: I also have huge windows. Maybe I will get to go on the other side one day. The morale of the story.......... Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Thursday, March 15, 2007 5:30 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: perfect histology lab I don't have to guess, I know I am lucky. I too am the lone histotech and do paraffin, frozens and plastics. I have worked with my supervisor for 17 years and you couldn't ask for a better person. I am left to my own devices as long as the results are good and on time. I can pretty much design my own schedule and yes, I have windows. Nirvana. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Proctor Sent: Wednesday, March 14, 2007 6:45 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: perfect histology lab I guess I am lucky; I run a histology core for an exclusive group of 6 scientists who are all studying neuroimmunology--and I'm the only person in my histology lab! The only thing expected of me is results, no drama to speak of. It's all my stuff, my mess. Perfect? I don't know, but it sure is nice. >>> "Steven Coakley" 03/14/07 4:23 PM >>> LOL, perfect histology lab ...ya right. How about a snot nose newbie tech right out of school, no clinical experience at all, running the show, spouting her theory and superior knowledge and intellect, brown nosing the boss that's now in her back pocket. All the experienced techs 14-40 years experience each moving right out the door. Go figure. And the boss can't understand why she can't keep techs. --------------------------------- Now that's room service! Choose from over 150,000 hotels in 45,000 destinations on Yahoo! Travel to find your fit. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GoodwinD <@t> pahosp.com Thu Mar 15 13:41:08 2007 From: GoodwinD <@t> pahosp.com (Goodwin, Diana) Date: Thu Mar 15 13:41:22 2007 Subject: [Histonet] NJSH Symposium Message-ID: <80CDD9C3FEEAFD4982B114C4A6DFD00E05905668@uphsmbx2.UPHS.PENNHEALTH.PRV> The NJSH March Symposium in Cherry Hill starts tomorrow!. We are anticipating a very exciting meeting with the largest selection of unique workshops ever offered at a New Jersey State meeting. All of the workshops have been approved for CEU credits. There has been an excellent response from both registrants and vendors interested in exhibiting. It is not too late to participate; we welcome walk-in registrations at the meeting. The full program and registration form can be found on our website at http://www.njshweb.org . There will be a cocktail reception on Friday evening with door prizes, wear your green and join us St. Patrick's Day weekend in Cherry Hill! We look forward to seeing you at the symposium. Best Regards, NJSH Joe Tamasi, President The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From Jason.Wiese <@t> va.gov Thu Mar 15 13:50:49 2007 From: Jason.Wiese <@t> va.gov (Wiese, Jason VHAROS) Date: Thu Mar 15 13:50:58 2007 Subject: [Histonet] CBG Biotech solvent recycler In-Reply-To: References: Message-ID: <70EEF3D43B3C164C94037D811B2BE193E12874@VHAV20MSGA3.v20.med.va.gov> I bought one in 2005. I got the 2 1/2 gallon bench top unit. I bought a "last years" model for around $9,000. In the time I have been using it, I have saved more then the price of the machine in reagent, disposal, and hazardous shipping costs. I have been using the same xylene for almost two years, and it works like it did the first day out of the bottle. My mixed alcohol usually comes back at 94 - 95%, and I use it straight out of the recycle jug. Also, CBG will do a cost comparison for you using your usage numbers. They have been good to me... If you have more questions you can call or email me... Jason Wiese, BS, HT(ASCP) Roseburg, OR 541-440-1000 Ext. 44751 jason.wiese@med.va.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roberta Horner Sent: Thursday, March 15, 2007 10:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CBG Biotech solvent recycler Does anyone use the Biotech recycler for alcohols and xylene substitutes? Could you tell me what you think about them? I have been trying to talk the higher ups about getting a recycler but am tired of jumping through hoops. Roberta Horner HT HTL Penn State University Animal Diagnostic Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GMartin <@t> marshallhospital.org Thu Mar 15 14:04:20 2007 From: GMartin <@t> marshallhospital.org (GMartin@marshallhospital.org) Date: Thu Mar 15 14:01:40 2007 Subject: [Histonet] recycle Message-ID: Speaking of recyclers ... does anybody have experience with the gravity fed bench top unit by Creative Waste Solutions? I will be recycling formalin and alcohol. I believe I heard that you need to buffer the recycled formalin ... please advise! Gary California From Jason.Wiese <@t> va.gov Thu Mar 15 14:10:22 2007 From: Jason.Wiese <@t> va.gov (Wiese, Jason VHAROS) Date: Thu Mar 15 14:10:43 2007 Subject: [Histonet] recycle In-Reply-To: References: Message-ID: <70EEF3D43B3C164C94037D811B2BE193E12876@VHAV20MSGA3.v20.med.va.gov> Any formalin you recycle, with any recycler, will have to be buffered... JW -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of GMartin@marshallhospital.org Sent: Thursday, March 15, 2007 12:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] recycle Speaking of recyclers ... does anybody have experience with the gravity fed bench top unit by Creative Waste Solutions? I will be recycling formalin and alcohol. I believe I heard that you need to buffer the recycled formalin ... please advise! Gary California _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From petunia_c <@t> hotmail.com Thu Mar 15 14:10:47 2007 From: petunia_c <@t> hotmail.com (barbara carter) Date: Thu Mar 15 14:11:04 2007 Subject: [Histonet] CBG Recycler Message-ID: Hi Roberta, The lab that I work for just purchased a recycler from CBG Biotech and we got the recycler that does the formalin, clearrite, and alcohol. I am the main user of the recycler and I am very impressed with the performance of it. We recycle the 100% and 95% alcohol seperately to get a better return with the 100% returning at a 98% to 99% and the 95% alcohol returning at a 94%. The clearrite has returned clean and not contaminated and our ordering of new reagnant has gone from weekly to once a month or longer. The 10% buffer formalin has been coming back at 11.4% but tech support from the company has brought the return to 10.7% by adjusting times. The company has a great tech support with any questions that you may have along the way. Barbara Carter HT ASCP Histology Coordinator Kenosha Medical Center _________________________________________________________________ Play Flexicon: the crossword game that feeds your brain. PLAY now for FREE.  http://zone.msn.com/en/flexicon/default.htm?icid=flexicon_hmtagline From cbobrowi <@t> mcw.edu Thu Mar 15 14:21:38 2007 From: cbobrowi <@t> mcw.edu (Bobrowitz, Carol) Date: Thu Mar 15 14:21:48 2007 Subject: [Histonet] DAKO Corporation Message-ID: <8F78639AC56F4143B267FE5F5A1B92C8F0ADE9@guyton.phys.mcw.edu> Hi, I just heard that Dako was sold. Does anyone have the inside scoop / gossip? It sure surprised me. I've worked with Dako products since 1981. Thanks, Carol Ann Bobrowitz Medical College of Wisconsin Physiology cbobrowi@mcw.edu From doug <@t> ppspath.com Thu Mar 15 15:33:30 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Thu Mar 15 14:31:17 2007 Subject: [Histonet] DAKO Corporation In-Reply-To: <8F78639AC56F4143B267FE5F5A1B92C8F0ADE9@guyton.phys.mcw.edu> Message-ID: Feb 28, 2007 Dako gets new owners Private equity fund EQT V (?EQT?) has signed a definitive agreement to acquire 100% of Dako, a leading Denmark-based supplier of systems for cancer diagnostics in pathology laboratories. The total consideration for the transaction is DKK 7.25 billion. Dako is one of the world?s leading developers, manufacturers and marketeers of systems for cancer diagnostics in pathology laboratories. In 2006 Dako generated sales of more than DKK 1,700 million, corresponding to a growth rate of 10% compared to 2005. With more than 1,250 employees and a presence in more than 20 countries, Dako covers most of the global pathology markets with reagents, instruments and software for high-quality, safe diagnosis. Dako is currently owned by the Harboe family (61%), Novo Nordisk (27%) and others (12%). The total consideration for the transaction is DKK 7.25 billion (enterprise value). ?The Dako Group has been a family-controlled business ever since its inception in 1966. The family and its shareholders have now decided to find a buyer who can take the company forward and further develop the business, maintaining the Dako name and brand. We are convinced that Dako and our employees will have greater growth potential with EQT as the new owner,? says Sonnich Fryland, Chairman of Dako?s Board of Directors. ?The Corporate Management of Dako is very satisfied that EQT, with its industrial approach, has acquired our company. Over the past two years we have worked hard to refocus the business of the Dako group to improve both growth and profitability. We now have a new growth strategy, we have new products in our pipeline, and with the support from EQT we will be able to make the investments necessary to accelerate our growth even further. Dako has the strongest brand name in the pathology market. By having industrial support from a financially strong owner, we will be able to expand our successful business model going forward,? says Patrik Dahl?n, CEO and President of Dako. The EQT equity funds have a strong track record of fostering growth and value creation in the companies they own. Over the past 12 years the funds have invested in 43 companies in Europe, resulting in an annual average increase in sales and number of employees of 11% during the EQT funds? ownership period. ?EQT is very pleased to add Dako into our portfolio. Dako has a powerful position in an exciting growth market in cancer diagnostics. Our intention is to support management in the further development of the company in accordance with the strategy as outlined by management. Dako will remain headquartered in Copenhagen, Denmark, and we will continue to build on the Dako brand in order to realize Dako?s full potential,? says Ole Andersen, Senior Partner at EQT Partners. The transaction is conditional upon the approval of the relevant competition authorities and the approval of the transaction by a shareholders meeting in Dako. Lehman Brothers is acting as exclusive financial advisor to the Dako Group and its shareholders. Bech Bruun is providing legal advice to the Dako Group and its shareholders. A new Board of Directors will be appointed by EQT and Dako?s Corporate Management. http://www.dako.com/index/press/press-list/press-item.htm?newsid=10934&g=tru e Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bobrowitz, Carol Sent: Thursday, March 15, 2007 2:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DAKO Corporation Hi, I just heard that Dako was sold. Does anyone have the inside scoop / gossip? It sure surprised me. I've worked with Dako products since 1981. Thanks, Carol Ann Bobrowitz Medical College of Wisconsin Physiology cbobrowi@mcw.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rgrow <@t> bmnet.com Thu Mar 15 14:32:57 2007 From: rgrow <@t> bmnet.com (rgrow@bmnet.com) Date: Thu Mar 15 14:33:15 2007 Subject: [Histonet] Re: CBG Biotech solvent recycler In-Reply-To: Message-ID: I have used both CBG and BR Instruments recyclers. Either is a good choice. You need to sell the "higher ups" on the environmental and cost savings. You can make all your diluted alcohols from the 95% recycled product. And the xylene (I use the real stuff) is better after recycling! Just smells different. One word of caution. Be sure to keep xylene and alcohol separated. You can never get xylene out of alcohol, but you can get alcohol out of xylene. The rep's will guide you. Happy Selling :-)) Renee Grow, BA., HT (ASCP) rgrow@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax Date: Thu, 15 Mar 2007 13:53:41 -0400 From: "Roberta Horner" Subject: [Histonet] CBG Biotech solvent recycler To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Does anyone use the Biotech recycler for alcohols and xylene substitutes? Could you tell me what you think about them? I have been trying to talk the higher ups about getting a recycler but am tired of jumping through hoops. Roberta Horner HT HTL Penn State University Animal Diagnostic Lab From laurie.colbert <@t> huntingtonhospital.com Thu Mar 15 15:59:42 2007 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Thu Mar 15 15:59:52 2007 Subject: [Histonet] recycle Message-ID: <57BE698966D5C54EAE8612E8941D7683199959@EXCHANGE3.huntingtonhospital.com> We have the Creative Waste Solution unit, and we love it! It is so easy to use. We do not have to buffer the recycled formalin. We periodically test the pH and the concentration, and I don't think we have ever had a problem with either. The support is very good - I would recommend the system! Laurie Colbert Huntington Hospital Pasadena, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of GMartin@marshallhospital.org Sent: Thursday, March 15, 2007 12:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] recycle Speaking of recyclers ... does anybody have experience with the gravity fed bench top unit by Creative Waste Solutions? I will be recycling formalin and alcohol. I believe I heard that you need to buffer the recycled formalin ... please advise! Gary California _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From darupke <@t> hotmail.com Thu Mar 15 17:28:32 2007 From: darupke <@t> hotmail.com (Debra Rupke) Date: Thu Mar 15 17:28:43 2007 Subject: [Histonet] Special stains cost per slide Message-ID: I am currently working on a cost analysis for Manual Special Staining vs. Automation. Does anyone know the” national average” cost per slide on manual staining. Any information would be appreciated. Deb Rupke H.T. (ASCP) _________________________________________________________________ The average US Credit Score is 675. The cost to see yours: $0 by Experian. http://www.freecreditreport.com/pm/default.aspx?sc=660600&bcd=EMAILFOOTERAVERAGE From gcallis <@t> montana.edu Thu Mar 15 18:20:45 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Mar 15 18:21:00 2007 Subject: [Histonet] Special stains cost per slide In-Reply-To: References: Message-ID: <6.0.0.22.1.20070315171343.01b6e790@gemini.msu.montana.edu> Check out Advance magazine for an article by Rene Buessa on histology laboratory cost evaluations (December, 2006 issue?) He should have insight on how this is calculated (a formula) or information on where to find what you are looking for. Advance is available on internet and free. If he is looking in, he will address your question. Also, check out the Archive, this has been discussed before on Histonet. At 04:28 PM 3/15/2007, you wrote: >I am currently working on a cost analysis for Manual Special Staining vs. >Automation. Does anyone know the" national average" cost per slide on >manual staining. Any information would be appreciated. > >Deb Rupke H.T. (ASCP) Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From Marilyn.Tyler <@t> uct.ac.za Fri Mar 16 00:19:42 2007 From: Marilyn.Tyler <@t> uct.ac.za (Marilyn Tyler) Date: Fri Mar 16 00:20:04 2007 Subject: [Histonet] thanks Message-ID: <45FA450E.A78E.0090.0@uct.ac.za> Hi All A big thank you for your input Marilyn From mkaulahao <@t> bellsouth.net Fri Mar 16 04:18:37 2007 From: mkaulahao <@t> bellsouth.net (mkaulahao) Date: Fri Mar 16 04:18:30 2007 Subject: [Histonet] perfect histo lab Message-ID: <000601c767ac$14ddbb20$6101a8c0@D8FNWG51> we just moved into a new lab. compared to the old one this one is better. better vents. no smells. BUT also no humidity. we haven't been able to get a ribbon when cutting since the move. so the perfect lab should have some humidity. and how did you get a job where the pathologist only comes once or twice a month?? mb From mkaulahao <@t> bellsouth.net Fri Mar 16 04:29:43 2007 From: mkaulahao <@t> bellsouth.net (mkaulahao@bellsouth.net) Date: Fri Mar 16 04:29:51 2007 Subject: [Histonet] histo lab Message-ID: <20070316092944.BAUK17681.ibm58aec.bellsouth.net@mail.bellsouth.net> steve, now i can't wait to get to work. the situation in your lab sounds horrible. there are only 2 of us. and 2 pathologists. i think the pathologists are slightly afraid of me. i wish you good luck with your boss. mb From NHeath <@t> Lifespan.org Fri Mar 16 05:16:01 2007 From: NHeath <@t> Lifespan.org (Heath, Nancy L.) Date: Fri Mar 16 05:16:10 2007 Subject: [Histonet] Thanks Everyone :) Message-ID: <130E8991F210424096EFC6F42EA33B2422405E@LSCOEXCH1.lsmaster.lifespan.org> Thanks to everyone who had a Jean Mitchell siting :) The real deal herself contacted me so her email address has been updated in my contacts. From louise.renton <@t> gmail.com Fri Mar 16 06:17:20 2007 From: louise.renton <@t> gmail.com (louise renton) Date: Fri Mar 16 06:17:32 2007 Subject: [Histonet] novolink kit Message-ID: hi all is anyone using the novolink kit sucessfully? I recently tried it out on antibodies that work fine with VEctor'snABC kit, only to find that there is absolutely NO staining. Any ideas? Best regrads & have a great weekend -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From atebo <@t> aahs.org Fri Mar 16 08:15:50 2007 From: atebo <@t> aahs.org (Tebo, Andrea) Date: Fri Mar 16 08:16:08 2007 Subject: [Histonet] Special stains cost per slide In-Reply-To: <6.0.0.22.1.20070315171343.01b6e790@gemini.msu.montana.edu> References: <6.0.0.22.1.20070315171343.01b6e790@gemini.msu.montana.edu> Message-ID: <00790F10D0600A41AEE8899901E533A61044B576@aamcexch.aamc.org> It is actually the January 2007 article. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Thursday, March 15, 2007 7:21 PM To: Debra Rupke; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Special stains cost per slide Check out Advance magazine for an article by Rene Buessa on histology laboratory cost evaluations (December, 2006 issue?) He should have insight on how this is calculated (a formula) or information on where to find what you are looking for. Advance is available on internet and free. If he is looking in, he will address your question. Also, check out the Archive, this has been discussed before on Histonet. At 04:28 PM 3/15/2007, you wrote: >I am currently working on a cost analysis for Manual Special Staining vs. >Automation. Does anyone know the" national average" cost per slide on >manual staining. Any information would be appreciated. > >Deb Rupke H.T. (ASCP) Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- This message [including any attachments] contains information intended for a specific individual[s] and purpose that may be confidential or otherwise legally protected from disclosure. Any review, use, distribution, disclosure of contents, or copying of the message is strictly prohibited. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately without copying or disclosing the information. From derek.papalegis <@t> tufts.edu Fri Mar 16 09:29:02 2007 From: derek.papalegis <@t> tufts.edu (Derek Papalegis) Date: Fri Mar 16 09:29:12 2007 Subject: [Histonet] Special stains cost per slide In-Reply-To: <00790F10D0600A41AEE8899901E533A61044B576@aamcexch.aamc.org> References: <6.0.0.22.1.20070315171343.01b6e790@gemini.msu.montana.edu> <00790F10D0600A41AEE8899901E533A61044B576@aamcexch.aamc.org> Message-ID: <45FAA9AE.9060504@tufts.edu> Does anybody have a link to where I can find this article on the net? I was unable to find it when I googled it. Tebo, Andrea wrote: > It is actually the January 2007 article. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle > Callis > Sent: Thursday, March 15, 2007 7:21 PM > To: Debra Rupke; Histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Special stains cost per slide > > Check out Advance magazine for an article by Rene Buessa on histology > laboratory cost evaluations (December, 2006 issue?) He should have > insight on how this is calculated (a formula) or information on where to > find what you are looking for. Advance is available on internet and > free. If he is looking in, he will address your question. > > Also, check out the Archive, this has been discussed before on Histonet. > > At 04:28 PM 3/15/2007, you wrote: > >> I am currently working on a cost analysis for Manual Special Staining >> > vs. > >> Automation. Does anyone know the" national average" cost per slide on >> manual staining. Any information would be appreciated. >> >> Deb Rupke H.T. (ASCP) >> > > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > 406 994-6367 > 406 994-4303 (FAX) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ---------------------------------------------------------------------- > This message [including any attachments] contains information intended for a specific individual[s] and purpose that may be confidential or otherwise legally protected from disclosure. Any review, use, distribution, disclosure of contents, or copying of the message is strictly prohibited. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately without copying or disclosing the information. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- From FUNKM <@t> mercyhealth.com Fri Mar 16 09:33:38 2007 From: FUNKM <@t> mercyhealth.com (Marcia Funk) Date: Fri Mar 16 09:34:05 2007 Subject: [Histonet] Film or glass Message-ID: <45FA6472020000AC00000736@nodcmsngwia1.trinity-health.org> Hello, I know you can help... I'm looking at glass or film coverslipper what do you find best about film or glass. We are using glass and still coversliping by hand... I know we are little behind with that part of our lab but you know how budget and Histo goes sometime... So if you can share I really would like to hear from you, I value your imput. Marcia From gcallis <@t> montana.edu Fri Mar 16 09:51:21 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Mar 16 09:51:37 2007 Subject: [Histonet] perfect histo lab In-Reply-To: <000601c767ac$14ddbb20$6101a8c0@D8FNWG51> References: <000601c767ac$14ddbb20$6101a8c0@D8FNWG51> Message-ID: <6.0.0.22.1.20070316085031.01b45a88@gemini.msu.montana.edu> Academic research laboratory At 03:18 AM 3/16/2007, you wrote: >we just moved into a new lab. compared to the old one this one is better. >better vents. no smells. BUT also no humidity. we haven't been able to get >a ribbon when cutting since the move. so the perfect lab should have some >humidity. and how did you get a job where the pathologist only comes once >or twice a month?? >mb >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 From rjbuesa <@t> yahoo.com Fri Mar 16 09:52:02 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Mar 16 09:52:12 2007 Subject: [Histonet] recycle In-Reply-To: Message-ID: <711377.3953.qm@web61215.mail.yahoo.com> Gary: In several occasions the topic of recycling formalin has been addressed in Histonet (you should try the files). I personally think it is a very bad idea because you will end with formalin without the buffer salts and you will have to work with the formalin, get in some way exposed to it, and will have to dilute it and buffer it, which will defeat the whole purpose of buying buffered formalin in the first place, i.e. not having to be exposed to this noxious chemical. To you question: yes, you will have to neutralize it after recycling it. Ren? J. GMartin@marshallhospital.org wrote: Speaking of recyclers ... does anybody have experience with the gravity fed bench top unit by Creative Waste Solutions? I will be recycling formalin and alcohol. I believe I heard that you need to buffer the recycled formalin ... please advise! Gary California _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- We won't tell. Get more on shows you hate to love (and love to hate): Yahoo! TV's Guilty Pleasures list. From MElliott <@t> mrl.ubc.ca Fri Mar 16 09:57:49 2007 From: MElliott <@t> mrl.ubc.ca (Mark Elliott) Date: Fri Mar 16 09:59:30 2007 Subject: [Histonet] Re: Histonet Digest, Vol 40, Issue 19 In-Reply-To: References: Message-ID: <45FA4DFD020000D600037946@mail.mrl.ubc.ca> As requested by a few people, here is the method for Hansel's Stain we have been using. It was originally modified to work on plastic sections but it will work on paraffin and cryosections as well. Mark Hansel*s Stain -developed for staining glutaraldehyde fixed, methacrylate embedded pulmonary tissues. This technique, developed by Randall J. Thomson and Laura L. Beattie during research under Dr. R.R. Schellenberg at the UBC Pulmonary Research Laboratory, is a modification of A.J.P. Scientific*s (1) method for staining eosinophils in unfixed, air dried urine samples for the detection of eosinophiluria (2). The protocol and results reported are based on guinea pig (Cam-Hartley) airway tissue fixed with glutaraldehyde (2.5% in 0.1 M Sodium Cacodylate buffer), embedded in glycol methacrylate (JB-4) plastic and sectioned at 3 um. Reagents: The reagents used can be easily prepared in the laboratory, or may be ordered fresh from A.J.P. Scientific (product # 692). Solution 1: 0.2% Eosin Y (C.I. 45380) in 100% methanol (3). (0.1g/50ml) Solution 2: 1:1 combination of Solution 1 and phosphate buffer (pH 6.8): Phosphate buffer, pH 6.8: Stock solution a: 9.465g Na2HPO4/L distilled water. Stock solution b: 9.070g KH2PO4/L distilled water. Use: 49.6 mL stock solution a + 50.4 mL stock solution b to yield 100 mL of buffer. Solution 3: 0.5% Methylene Blue (C.I. 52015) in 100% methanol (3). (0.25g/50ml) ** for cryosections and cytospins use 0.05-0.005% methylene blue ** for paraffin use 0.025 g/50 ml or use 0.25g/50ml but use solution 3 for 1 sec, rinse until clear, air dry and coverslip. Protocol: 1. Submerge slides in Solution 1 for 60 seconds. 2. Transfer to Solution 2 for 5 minutes. 3. Pass slides through two rapid distilled water washes to clear excess Eosin Y, and allow as much water to drain off as possible before proceeding to next step. 4. Submerge slides in Solution 3 for 8 seconds. 5. Immediately pass slides through two distilled water washes to clear excess Methylene Blue, and allow slides to air dry IN THE DARK. 6. Coverslip slides and view. Results: Eosinophils stain with pale red cytoplasm and bright red granules, nuclei stain dark blue, epithelial and smooth muscle cells stain with medium blue cytoplasm, connective tissue stains with light blue cytoplasm, mast cell granules stain deep purple, and chondrocyte cytoplasm and the bulk of the cartilage matrix stain violet. (Eosin stains argenine residues in *eosinophilic* granules). If doing paraffin sections, one can dehydrate to clear as usual, and then mount with permanent mounting medium. Warning: Empirical studies have shown that extended exposure of the slides to an intense light source (such as that of a projecting microscope) can cause a photo-reaction leading to a loss of definition of staining (the mechanism of this effect is unclear), while slides kept in the dark (while not in use with a standard light microscope) are stable for at least 5 months (our present maximum storage period). Notes: 1 A.J.P. Hansel stain kit insert, A.J.P. Scientific, Inc., PO Box 1589, Union City NJ Telephone (201) 472-7200. 2 Nolan, C.R., III, M.S. Anger and S.P. Kelleher. Eosinophiluria*A new method of detectin and definition of the clinical spectrum. N. Engl. J. Med. 315:1516-1519, 1986. 3 Use a transparent brown or opaque glass bottle for storage. Original reference is Hansel FK. Clinical Allergy. St. Louis: CV Mosby, 1953. Our reference: Matsuse T, Thomson RJ, Chen X-R, Salari H and RR Schellenberg (1991). Capsaicin inhibits airway hyper-responsiveness but not lipoxygenase activity or eosinophilia after repeated aerosolized antigen in guinea pigs. Am. Rev. Respir. Dis. 144:368-372. ***CONFIDENTIALITY NOTICE*** This electronic message is intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, dis tribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From rjbuesa <@t> yahoo.com Fri Mar 16 10:04:50 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Mar 16 10:04:59 2007 Subject: [Histonet] Special stains cost per slide In-Reply-To: Message-ID: <514240.42066.qm@web61211.mail.yahoo.com> Debra: As Gayle wrote you, I am sending you my article on costs that include not only special stains, but many other histology procedures. Ren? J. Debra Rupke wrote: I am currently working on a cost analysis for Manual Special Staining vs. Automation. Does anyone know the? national average? cost per slide on manual staining. Any information would be appreciated. Deb Rupke H.T. (ASCP) _________________________________________________________________ The average US Credit Score is 675. The cost to see yours: $0 by Experian. http://www.freecreditreport.com/pm/default.aspx?sc=660600&bcd=EMAILFOOTERAVERAGE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a PS3 game guru. Get your game face on with the latest PS3 news and previews at Yahoo! Games. From tkngflght <@t> yahoo.com Fri Mar 16 10:12:11 2007 From: tkngflght <@t> yahoo.com (Cheryl Kerry) Date: Fri Mar 16 10:12:23 2007 Subject: [Histonet] Film or glass In-Reply-To: <45FA6472020000AC00000736@nodcmsngwia1.trinity-health.org> References: <45FA6472020000AC00000736@nodcmsngwia1.trinity-health.org> Message-ID: <002301c767dd$791b63e0$6501a8c0@FSDESKTOP> Marcia- There are advantages to both. These are, of course, my opinions: Film is simpler/faster but has some disadvantages- -the machine has less moving parts to break -if you run out of film, you go back to hand coverslipping--should maintain supplies just in case. -there are a few tricks to it--acclimate the film to ambient room temp for a day before using. -Keep the machine CLEAN or it bunches up. -If your storage isn't climate controlled you'll have trouble with yellowing and rarely, fogging. -The slip is hard to remove--acetone works but the sludge is messy and it leaves a residue. -On thick preps (cytos)it is harder to fill in the bubbles. Glass slips -The machine is more complex and a little harder to maintain -You can use anyone's slips--no limited suppliers -The machine is more expensive, but supplies are less -Storage--no issues and no adjustments by your pathologists. -If slips break in the machine--they're a pain to clean up. I know I listed fewer issues for the glass--it's obviously my preferred choice. Hope this helps! Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. 281.852.9457 800.756.3309 Sign up for the FREE newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcia Funk Sent: Friday, March 16, 2007 9:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Film or glass Hello, I know you can help... I'm looking at glass or film coverslipper what do you find best about film or glass. We are using glass and still coversliping by hand... I know we are little behind with that part of our lab but you know how budget and Histo goes sometime... So if you can share I really would like to hear from you, I value your imput. Marcia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Mar 16 10:23:54 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Mar 16 10:24:02 2007 Subject: [Histonet] Film or glass In-Reply-To: <45FA6472020000AC00000736@nodcmsngwia1.trinity-health.org> Message-ID: <307902.78291.qm@web61214.mail.yahoo.com> Marcia: Manual coverslipping is expensive because of the time it requires to have histotechs doing the task, so any lab trying to increase productivity should automat this step of the work flow. For maximum speed and convenience you should go with film coverslipping BUT some pathologists don't like it. Their reasons are that some say that for phtomicrographs glass is better (and they are WRONG in that) or that archival slides with film will deteriorate faster and may lose the film (and they are RIGHT in that). A regular objective (20:1 or 40:1) has a non critical NA (numerical apperture) and will define well with the light coming through the film, BUT if the amount of xylene is below what is needed, the film can separate from the section after some time. So I would recommend you to talk to your pathologists before selection, but you should automat this step anyway. Hope this will help you! Ren? J. Marcia Funk wrote: Hello, I know you can help... I'm looking at glass or film coverslipper what do you find best about film or glass. We are using glass and still coversliping by hand... I know we are little behind with that part of our lab but you know how budget and Histo goes sometime... So if you can share I really would like to hear from you, I value your imput. Marcia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Don't pick lemons. See all the new 2007 cars at Yahoo! Autos. From doug <@t> ppspath.com Fri Mar 16 11:26:46 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Fri Mar 16 10:24:45 2007 Subject: [Histonet] Film or glass In-Reply-To: <002301c767dd$791b63e0$6501a8c0@FSDESKTOP> Message-ID: There is virtually no drying time needed for the tape to be filed. I also find the tape easier to remove (using acetone) vs. the dried glass slide. Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Kerry Sent: Friday, March 16, 2007 10:12 AM To: 'Marcia Funk'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Film or glass Marcia- There are advantages to both. These are, of course, my opinions: Film is simpler/faster but has some disadvantages- -the machine has less moving parts to break -if you run out of film, you go back to hand coverslipping--should maintain supplies just in case. -there are a few tricks to it--acclimate the film to ambient room temp for a day before using. -Keep the machine CLEAN or it bunches up. -If your storage isn't climate controlled you'll have trouble with yellowing and rarely, fogging. -The slip is hard to remove--acetone works but the sludge is messy and it leaves a residue. -On thick preps (cytos)it is harder to fill in the bubbles. Glass slips -The machine is more complex and a little harder to maintain -You can use anyone's slips--no limited suppliers -The machine is more expensive, but supplies are less -Storage--no issues and no adjustments by your pathologists. -If slips break in the machine--they're a pain to clean up. I know I listed fewer issues for the glass--it's obviously my preferred choice. Hope this helps! Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. 281.852.9457 800.756.3309 Sign up for the FREE newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcia Funk Sent: Friday, March 16, 2007 9:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Film or glass Hello, I know you can help... I'm looking at glass or film coverslipper what do you find best about film or glass. We are using glass and still coversliping by hand... I know we are little behind with that part of our lab but you know how budget and Histo goes sometime... So if you can share I really would like to hear from you, I value your imput. Marcia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jmahoney <@t> alegent.org Fri Mar 16 10:25:08 2007 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Fri Mar 16 10:25:30 2007 Subject: [Histonet] Pre-labeling glass slides In-Reply-To: <002601c76518$496450c0$6a9a9618@Katri> References: <893017.12787.qm@web61221.mail.yahoo.com> <002601c76518$496450c0$6a9a9618@Katri> Message-ID: <45FA70840200003C0000844A@gwia.alegent.org> I can't keep quiet anymore. Single piece flow, one specimen, slide, case at a time is the fastest and least error prone way of performing any task. It is LEAN and it can be proven. Please consider it for the patient's sake. i know that it seems more efficient to batch. I felt that way too before I started seeing the processes in my lab with LEAN eyes. All those checks along the way are slowing down your process. If you single piece flow, one case/slide at at time you will make fewer errors and no longer need so many checks. You will get more slides out faster (with the right number on them whether they are written or etched or labelled). Jan Omaha Janice Mahoney Histology/Cytology Coordinator Alegent Health Laboratory 4955 F Street Omaha, NE 68117 (402)717-2889 >>> "Katri Tuomala" 03/12/2007 9:35 PM >>> I totally agree with Rene. We pre-label all our slides in approximately 20 slide lots. We have so many checks along the way, before the slides are delivered, that the odd mistake gets discovered very quickly. You just have to pay attention to what you are doing. And yes, I remember the diamond pens, ouch!! Katri Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "Rene J Buesa" To: "Carmen Leschuk" ; Sent: Monday, March 12, 2007 12:44 PM Subject: Re: [Histonet] Pre-labeling glass slides > Carmen: > Labelling the slides BEFORE they are used is the normal and logical > practice. In this way the slides can be ready beforehand. The thing is > that if the person using the slides does NOT pay attention, the > "misslabelling" can occur. > On the other hand the amount of time you could WASTE by leballing > simultaneously while sectioning is astronomical. > The good practice is to have a log with the blocks to section, prepare > all the labelled slides in the needed amount and BEFORE sectioning the > blocks, match slides to blocks and that is all you have to do. > Sloppines and carelessnes are the roots of any mistake in histology. The > solution is to pay attention, not to misuse a slides writing machine. > Ren? J. > > Carmen Leschuk wrote: > Recently, I heard of a slide mislabeling that occurred in another histo > lab that they determined to be caused by pre-labeling slides before actual > use at the microtome. This lab said that if the slides would of been > labeled simultaneously (per their policy) as the blocks being cut, the > mislabeling would of been prevented. My lab currently pre-labels all of > slides before cutting a sequence of 10-20 blocks, which I thought was > common practice. My question is, what is common practice? Do other labs > have poli > cies forbidding pre-labeling of glass slides? > > > > Carmen Leschuk, HT, SLS (ASCP) > Supervisor, SJMO-Anatomic Pathology > (248)858-6231 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Looking for earth-friendly autos? > Browse Top Cars by "Green Rating" at Yahoo! Autos' Green Center. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Fri Mar 16 11:40:57 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Fri Mar 16 10:40:00 2007 Subject: [Histonet] Pre-labeling glass slides In-Reply-To: <45FA70840200003C0000844A@gwia.alegent.org> Message-ID: So it will save us time by having the tech get up and print the slide (using the slide printer), go back to the microtome and cut it. This will happen about 300+ times a night. Let's put two techs into the equation and one will wait while the other is printing. If they do this I will not have to check the slides for accuracy anymore? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: Friday, March 16, 2007 10:25 AM To: Katri Tuomala; histonet@lists.utsouthwestern.edu; Carmen Leschuk; Rene J Buesa Subject: Re: [Histonet] Pre-labeling glass slides I can't keep quiet anymore. Single piece flow, one specimen, slide, case at a time is the fastest and least error prone way of performing any task. It is LEAN and it can be proven. Please consider it for the patient's sake. i know that it seems more efficient to batch. I felt that way too before I started seeing the processes in my lab with LEAN eyes. All those checks along the way are slowing down your process. If you single piece flow, one case/slide at at time you will make fewer errors and no longer need so many checks. You will get more slides out faster (with the right number on them whether they are written or etched or labelled). Jan Omaha Janice Mahoney Histology/Cytology Coordinator Alegent Health Laboratory 4955 F Street Omaha, NE 68117 (402)717-2889 >>> "Katri Tuomala" 03/12/2007 9:35 PM >>> I totally agree with Rene. We pre-label all our slides in approximately 20 slide lots. We have so many checks along the way, before the slides are delivered, that the odd mistake gets discovered very quickly. You just have to pay attention to what you are doing. And yes, I remember the diamond pens, ouch!! Katri Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "Rene J Buesa" To: "Carmen Leschuk" ; Sent: Monday, March 12, 2007 12:44 PM Subject: Re: [Histonet] Pre-labeling glass slides > Carmen: > Labelling the slides BEFORE they are used is the normal and logical > practice. In this way the slides can be ready beforehand. The thing is > that if the person using the slides does NOT pay attention, the > "misslabelling" can occur. > On the other hand the amount of time you could WASTE by leballing > simultaneously while sectioning is astronomical. > The good practice is to have a log with the blocks to section, prepare > all the labelled slides in the needed amount and BEFORE sectioning the > blocks, match slides to blocks and that is all you have to do. > Sloppines and carelessnes are the roots of any mistake in histology. The > solution is to pay attention, not to misuse a slides writing machine. > Ren? J. > > Carmen Leschuk wrote: > Recently, I heard of a slide mislabeling that occurred in another histo > lab that they determined to be caused by pre-labeling slides before actual > use at the microtome. This lab said that if the slides would of been > labeled simultaneously (per their policy) as the blocks being cut, the > mislabeling would of been prevented. My lab currently pre-labels all of > slides before cutting a sequence of 10-20 blocks, which I thought was > common practice. My question is, what is common practice? Do other labs > have poli > cies forbidding pre-labeling of glass slides? > > > > Carmen Leschuk, HT, SLS (ASCP) > Supervisor, SJMO-Anatomic Pathology > (248)858-6231 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Looking for earth-friendly autos? > Browse Top Cars by "Green Rating" at Yahoo! Autos' Green Center. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Fri Mar 16 11:17:13 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Fri Mar 16 11:17:24 2007 Subject: [Histonet] Re: Histonet Digest, Vol 40, Issue 19 In-Reply-To: <45FA4DFD020000D600037946@mail.mrl.ubc.ca> References: <45FA4DFD020000D600037946@mail.mrl.ubc.ca> Message-ID: Has anybody else tried Gretel's Stain for marzipan yet?, worked a treat for us!. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Elliott Sent: 16 March 2007 14:58 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 40, Issue 19 As requested by a few people, here is the method for Hansel's Stain we have been using. It was originally modified to work on plastic sections but it will work on paraffin and cryosections as well. Mark Hansel*s Stain -developed for staining glutaraldehyde fixed, methacrylate embedded pulmonary tissues. This technique, developed by Randall J. Thomson and Laura L. Beattie during research under Dr. R.R. Schellenberg at the UBC Pulmonary Research Laboratory, is a modification of A.J.P. Scientific*s (1) method for staining eosinophils in unfixed, air dried urine samples for the detection of eosinophiluria (2). The protocol and results reported are based on guinea pig (Cam-Hartley) airway tissue fixed with glutaraldehyde (2.5% in 0.1 M Sodium Cacodylate buffer), embedded in glycol methacrylate (JB-4) plastic and sectioned at 3 um. Reagents: The reagents used can be easily prepared in the laboratory, or may be ordered fresh from A.J.P. Scientific (product # 692). Solution 1: 0.2% Eosin Y (C.I. 45380) in 100% methanol (3). (0.1g/50ml) Solution 2: 1:1 combination of Solution 1 and phosphate buffer (pH 6.8): Phosphate buffer, pH 6.8: Stock solution a: 9.465g Na2HPO4/L distilled water. Stock solution b: 9.070g KH2PO4/L distilled water. Use: 49.6 mL stock solution a + 50.4 mL stock solution b to yield 100 mL of buffer. Solution 3: 0.5% Methylene Blue (C.I. 52015) in 100% methanol (3). (0.25g/50ml) ** for cryosections and cytospins use 0.05-0.005% methylene blue ** for paraffin use 0.025 g/50 ml or use 0.25g/50ml but use solution 3 for 1 sec, rinse until clear, air dry and coverslip. Protocol: 1. Submerge slides in Solution 1 for 60 seconds. 2. Transfer to Solution 2 for 5 minutes. 3. Pass slides through two rapid distilled water washes to clear excess Eosin Y, and allow as much water to drain off as possible before proceeding to next step. 4. Submerge slides in Solution 3 for 8 seconds. 5. Immediately pass slides through two distilled water washes to clear excess Methylene Blue, and allow slides to air dry IN THE DARK. 6. Coverslip slides and view. Results: Eosinophils stain with pale red cytoplasm and bright red granules, nuclei stain dark blue, epithelial and smooth muscle cells stain with medium blue cytoplasm, connective tissue stains with light blue cytoplasm, mast cell granules stain deep purple, and chondrocyte cytoplasm and the bulk of the cartilage matrix stain violet. (Eosin stains argenine residues in *eosinophilic* granules). If doing paraffin sections, one can dehydrate to clear as usual, and then mount with permanent mounting medium. Warning: Empirical studies have shown that extended exposure of the slides to an intense light source (such as that of a projecting microscope) can cause a photo-reaction leading to a loss of definition of staining (the mechanism of this effect is unclear), while slides kept in the dark (while not in use with a standard light microscope) are stable for at least 5 months (our present maximum storage period). Notes: 1 A.J.P. Hansel stain kit insert, A.J.P. Scientific, Inc., PO Box 1589, Union City NJ Telephone (201) 472-7200. 2 Nolan, C.R., III, M.S. Anger and S.P. Kelleher. Eosinophiluria*A new method of detectin and definition of the clinical spectrum. N. Engl. J. Med. 315:1516-1519, 1986. 3 Use a transparent brown or opaque glass bottle for storage. Original reference is Hansel FK. Clinical Allergy. St. Louis: CV Mosby, 1953. Our reference: Matsuse T, Thomson RJ, Chen X-R, Salari H and RR Schellenberg (1991). Capsaicin inhibits airway hyper-responsiveness but not lipoxygenase activity or eosinophilia after repeated aerosolized antigen in guinea pigs. Am. Rev. Respir. Dis. 144:368-372. ***CONFIDENTIALITY NOTICE*** This electronic message is intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, dis tribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Mar 16 11:23:40 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Mar 16 11:23:47 2007 Subject: [Histonet] Pre-labeling glass slides Message-ID: <923477.1710.qm@web61222.mail.yahoo.com> As it hapened with Janice I can't keep quiet either. Lets talk about LEAN. To start with it is not applicable to the entire histology workflow. The specimen arrives and somebody takes "hold" of it, number, access, describe AND??? that person will have to process it (unless it is a Frozen section). There has to be a delay and don't tell about the Xpress Sakura, it will have to wait until 30 or 40 cassettes will be ready before starting (and what do you call that if you don't want to call it a "batch"?) It will come out after 120 minutes (or more time if it is a regular processor) and again another person will take the cassette in a "nurturing and loving embrace" and will prepare the slide, embed the tissue, section it, WAIT until it dries and will stain it. How long will it take to that "dedicated" histotech to have the slide ready for the pathologist and, most important, how much did that slide cost? What happened to the other cases? Is it a "one specimen a day"lab? Trying to see the histology lab as the production line of a Toyota is unrealistic. Some aspects can be streamlined, others not. The only process that is LEAN (and has always been without knowing it by the way) is the one performed by the histotech rushing over the piece of tissue received from the OR to do a FS, and even there I have seen some mislabellings (like some labs that do not access the tissue to do the FS). Embracing new approaches require a deep study and knowing what we are getting into. Just my opinion (as always)! Ren? J. Douglas D Deltour wrote: So it will save us time by having the tech get up and print the slide (using the slide printer), go back to the microtome and cut it. This will happen about 300+ times a night. Let's put two techs into the equation and one will wait while the other is printing. If they do this I will not have to check the slides for accuracy anymore? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: Friday, March 16, 2007 10:25 AM To: Katri Tuomala; histonet@lists.utsouthwestern.edu; Carmen Leschuk; Rene J Buesa Subject: Re: [Histonet] Pre-labeling glass slides I can't keep quiet anymore. Single piece flow, one specimen, slide, case at a time is the fastest and least error prone way of performing any task. It is LEAN and it can be proven. Please consider it for the patient's sake. i know that it seems more efficient to batch. I felt that way too before I started seeing the processes in my lab with LEAN eyes. All those checks along the way are slowing down your process. If you single piece flow, one case/slide at at time you will make fewer errors and no longer need so many checks. You will get more slides out faster (with the right number on them whether they are written or etched or labelled). Jan Omaha Janice Mahoney Histology/Cytology Coordinator Alegent Health Laboratory 4955 F Street Omaha, NE 68117 (402)717-2889 >>> "Katri Tuomala" 03/12/2007 9:35 PM >>> I totally agree with Rene. We pre-label all our slides in approximately 20 slide lots. We have so many checks along the way, before the slides are delivered, that the odd mistake gets discovered very quickly. You just have to pay attention to what you are doing. And yes, I remember the diamond pens, ouch!! Katri Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "Rene J Buesa" To: "Carmen Leschuk" ; Sent: Monday, March 12, 2007 12:44 PM Subject: Re: [Histonet] Pre-labeling glass slides > Carmen: > Labelling the slides BEFORE they are used is the normal and logical > practice. In this way the slides can be ready beforehand. The thing is > that if the person using the slides does NOT pay attention, the > "misslabelling" can occur. > On the other hand the amount of time you could WASTE by leballing > simultaneously while sectioning is astronomical. > The good practice is to have a log with the blocks to section, prepare > all the labelled slides in the needed amount and BEFORE sectioning the > blocks, match slides to blocks and that is all you have to do. > Sloppines and carelessnes are the roots of any mistake in histology. The > solution is to pay attention, not to misuse a slides writing machine. > Ren? J. > > Carmen Leschuk wrote: > Recently, I heard of a slide mislabeling that occurred in another histo > lab that they determined to be caused by pre-labeling slides before actual > use at the microtome. This lab said that if the slides would of been > labeled simultaneously (per their policy) as the blocks being cut, the > mislabeling would of been prevented. My lab currently pre-labels all of > slides before cutting a sequence of 10-20 blocks, which I thought was > common practice. My question is, what is common practice? Do other labs > have poli > cies forbidding pre-labeling of glass slides? > > > > Carmen Leschuk, HT, SLS (ASCP) > Supervisor, SJMO-Anatomic Pathology > (248)858-6231 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Looking for earth-friendly autos? > Browse Top Cars by "Green Rating" at Yahoo! Autos' Green Center. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss an email again! Yahoo! Toolbar alerts you the instant new Mail arrives. Check it out. From pruegg <@t> ihctech.net Fri Mar 16 11:33:38 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Mar 16 11:33:51 2007 Subject: [Histonet] perfect histo lab In-Reply-To: <6.0.0.22.1.20070316085031.01b45a88@gemini.msu.montana.edu> Message-ID: <002801c767e8$db266a70$6501a8c0@Patsy> For me the "Perfect" histo lab is my lab that I own, I have complete control. Of course there are things that I would like but cannot afford but I have what I need to do what I do my way and no body to tell me to do it their way. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Friday, March 16, 2007 8:51 AM To: mkaulahao; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] perfect histo lab Academic research laboratory At 03:18 AM 3/16/2007, you wrote: >we just moved into a new lab. compared to the old one this one is better. >better vents. no smells. BUT also no humidity. we haven't been able to get >a ribbon when cutting since the move. so the perfect lab should have some >humidity. and how did you get a job where the pathologist only comes once >or twice a month?? >mb >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jmahoney <@t> alegent.org Fri Mar 16 11:38:22 2007 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Fri Mar 16 11:38:50 2007 Subject: [Histonet] Pre-labeling glass slides In-Reply-To: <20070316154009.E8E5F61BB5C@mail63-cpk.bigfish.com> References: <45FA70840200003C0000844A@gwia.alegent.org> <20070316154009.E8E5F61BB5C@mail63-cpk.bigfish.com> Message-ID: <45FA81AE0200003C00008593@gwia.alegent.org> I'm just saying that there are ways around this process. Consider things like moving your labeler closer to the cutting station for instance, or having someone other than a HT making and delivering the slides or maybe getting several slide labelers and putting them by the cutting stations. I totally agree that having tech get up and walk to another place to get slides is not efficient and having people standing and waiting to do work is even worse. I'm just saying that there are probably ways to make your work flow better and more efficiently by using LEAN principles. It is too complex to go into in an email but I encourage you to do some investigating and reading on the subject and you may discover some good ideas. Jan >>> "Douglas D Deltour" 03/16/2007 11:40 AM >>> So it will save us time by having the tech get up and print the slide (using the slide printer), go back to the microtome and cut it. This will happen about 300+ times a night. Let's put two techs into the equation and one will wait while the other is printing. If they do this I will not have to check the slides for accuracy anymore? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: Friday, March 16, 2007 10:25 AM To: Katri Tuomala; histonet@lists.utsouthwestern.edu; Carmen Leschuk; Rene J Buesa Subject: Re: [Histonet] Pre-labeling glass slides I can't keep quiet anymore. Single piece flow, one specimen, slide, case at a time is the fastest and least error prone way of performing any task. It is LEAN and it can be proven. Please consider it for the patient's sake. i know that it seems more efficient to batch. I felt that way too before I started seeing the processes in my lab with LEAN eyes. All those checks along the way are slowing down your process. If you single piece flow, one case/slide at at time you will make fewer errors and no longer need so many checks. You will get more slides out faster (with the right number on them whether they are written or etched or labelled). Jan Omaha Janice Mahoney Histology/Cytology Coordinator Alegent Health Laboratory 4955 F Street Omaha, NE 68117 (402)717-2889 >>> "Katri Tuomala" 03/12/2007 9:35 PM >>> I totally agree with Rene. We pre-label all our slides in approximately 20 slide lots. We have so many checks along the way, before the slides are delivered, that the odd mistake gets discovered very quickly. You just have to pay attention to what you are doing. And yes, I remember the diamond pens, ouch!! Katri Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "Rene J Buesa" To: "Carmen Leschuk" ; Sent: Monday, March 12, 2007 12:44 PM Subject: Re: [Histonet] Pre-labeling glass slides > Carmen: > Labelling the slides BEFORE they are used is the normal and logical > practice. In this way the slides can be ready beforehand. The thing is > that if the person using the slides does NOT pay attention, the > "misslabelling" can occur. > On the other hand the amount of time you could WASTE by leballing > simultaneously while sectioning is astronomical. > The good practice is to have a log with the blocks to section, prepare > all the labelled slides in the needed amount and BEFORE sectioning the > blocks, match slides to blocks and that is all you have to do. > Sloppines and carelessnes are the roots of any mistake in histology. The > solution is to pay attention, not to misuse a slides writing machine. > Ren? J. > > Carmen Leschuk wrote: > Recently, I heard of a slide mislabeling that occurred in another histo > lab that they determined to be caused by pre-labeling slides before actual > use at the microtome. This lab said that if the slides would of been > labeled simultaneously (per their policy) as the blocks being cut, the > mislabeling would of been prevented. My lab currently pre-labels all of > slides before cutting a sequence of 10-20 blocks, which I thought was > common practice. My question is, what is common practice? Do other labs > have poli > cies forbidding pre-labeling of glass slides? > > > > Carmen Leschuk, HT, SLS (ASCP) > Supervisor, SJMO-Anatomic Pathology > (248)858-6231 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Looking for earth-friendly autos? > Browse Top Cars by "Green Rating" at Yahoo! Autos' Green Center. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Fri Mar 16 12:47:29 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Fri Mar 16 11:45:17 2007 Subject: [Histonet] Pre-labeling glass slides In-Reply-To: <45FA81AE0200003C00008593@gwia.alegent.org> Message-ID: I am familiar with the whole LEAN concept and the seven types of waste. The point is that it is not going to work in the majority of histology labs unless you build it from scratch applying LEAN. I know I don't have room for a slide labeler next to a microtome. I love how concepts are applied in the medical field that comes from auto makers. Healthcare administrators eat this stuff up and try to apply it to everything. Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: Janice Mahoney [mailto:jmahoney@alegent.org] Sent: Friday, March 16, 2007 11:38 AM To: 'Katri Tuomala'; histonet@lists.utsouthwestern.edu; Douglas D Deltour; 'Carmen Leschuk'; 'Rene J Buesa' Subject: RE: [Histonet] Pre-labeling glass slides I'm just saying that there are ways around this process. Consider things like moving your labeler closer to the cutting station for instance, or having someone other than a HT making and delivering the slides or maybe getting several slide labelers and putting them by the cutting stations. I totally agree that having tech get up and walk to another place to get slides is not efficient and having people standing and waiting to do work is even worse. I'm just saying that there are probably ways to make your work flow better and more efficiently by using LEAN principles. It is too complex to go into in an email but I encourage you to do some investigating and reading on the subject and you may discover some good ideas. Jan >>> "Douglas D Deltour" 03/16/2007 11:40 AM >>> So it will save us time by having the tech get up and print the slide (using the slide printer), go back to the microtome and cut it. This will happen about 300+ times a night. Let's put two techs into the equation and one will wait while the other is printing. If they do this I will not have to check the slides for accuracy anymore? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: Friday, March 16, 2007 10:25 AM To: Katri Tuomala; histonet@lists.utsouthwestern.edu; Carmen Leschuk; Rene J Buesa Subject: Re: [Histonet] Pre-labeling glass slides I can't keep quiet anymore. Single piece flow, one specimen, slide, case at a time is the fastest and least error prone way of performing any task. It is LEAN and it can be proven. Please consider it for the patient's sake. i know that it seems more efficient to batch. I felt that way too before I started seeing the processes in my lab with LEAN eyes. All those checks along the way are slowing down your process. If you single piece flow, one case/slide at at time you will make fewer errors and no longer need so many checks. You will get more slides out faster (with the right number on them whether they are written or etched or labelled). Jan Omaha Janice Mahoney Histology/Cytology Coordinator Alegent Health Laboratory 4955 F Street Omaha, NE 68117 (402)717-2889 >>> "Katri Tuomala" 03/12/2007 9:35 PM >>> I totally agree with Rene. We pre-label all our slides in approximately 20 slide lots. We have so many checks along the way, before the slides are delivered, that the odd mistake gets discovered very quickly. You just have to pay attention to what you are doing. And yes, I remember the diamond pens, ouch!! Katri Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "Rene J Buesa" To: "Carmen Leschuk" ; Sent: Monday, March 12, 2007 12:44 PM Subject: Re: [Histonet] Pre-labeling glass slides > Carmen: > Labelling the slides BEFORE they are used is the normal and logical > practice. In this way the slides can be ready beforehand. The thing is > that if the person using the slides does NOT pay attention, the > "misslabelling" can occur. > On the other hand the amount of time you could WASTE by leballing > simultaneously while sectioning is astronomical. > The good practice is to have a log with the blocks to section, prepare > all the labelled slides in the needed amount and BEFORE sectioning the > blocks, match slides to blocks and that is all you have to do. > Sloppines and carelessnes are the roots of any mistake in histology. The > solution is to pay attention, not to misuse a slides writing machine. > Ren? J. > > Carmen Leschuk wrote: > Recently, I heard of a slide mislabeling that occurred in another histo > lab that they determined to be caused by pre-labeling slides before actual > use at the microtome. This lab said that if the slides would of been > labeled simultaneously (per their policy) as the blocks being cut, the > mislabeling would of been prevented. My lab currently pre-labels all of > slides before cutting a sequence of 10-20 blocks, which I thought was > common practice. My question is, what is common practice? Do other labs > have poli > cies forbidding pre-labeling of glass slides? > > > > Carmen Leschuk, HT, SLS (ASCP) > Supervisor, SJMO-Anatomic Pathology > (248)858-6231 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Looking for earth-friendly autos? > Browse Top Cars by "Green Rating" at Yahoo! Autos' Green Center. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Margaret.Perry <@t> sdstate.edu Fri Mar 16 11:59:03 2007 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Fri Mar 16 11:59:15 2007 Subject: [Histonet] bird antibodies Message-ID: I have been asked to work up IHC on FFPE tissue on birds for newcastle disease, Infectious laryngotracheitis and Infectious bursal disease. Does anyone have a good source for these antibodies? I have found infectious bursal disease from US biological but it is produced in chicken. Would'nt I have the same problems as mouse on mouse? Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 From ftulenko06 <@t> jcu.edu Fri Mar 16 12:29:22 2007 From: ftulenko06 <@t> jcu.edu (ftulenko06@jcu.edu) Date: Fri Mar 16 12:29:31 2007 Subject: [Histonet] Antibody 3A10 Message-ID: <20070316132922.AAH33748@mirapoint.jcu.edu> Hello All, Does anyone have a good protocol for staining tissue sections (paraplast) of PFA fixed mice with 3A10 (an anti-neurofilament antibody)? Thank you for your advice. Frank From slappycraw <@t> yahoo.com Fri Mar 16 12:39:12 2007 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Fri Mar 16 12:39:22 2007 Subject: [Histonet] perfect histo lab In-Reply-To: <002801c767e8$db266a70$6501a8c0@Patsy> Message-ID: <20070316173912.68807.qmail@web53602.mail.yahoo.com> The perfect lab would run completely automated so I could be out catching the perfect wave Patsy Ruegg wrote: For me the "Perfect" histo lab is my lab that I own, I have complete control. Of course there are things that I would like but cannot afford but I have what I need to do what I do my way and no body to tell me to do it their way. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Friday, March 16, 2007 8:51 AM To: mkaulahao; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] perfect histo lab Academic research laboratory At 03:18 AM 3/16/2007, you wrote: >we just moved into a new lab. compared to the old one this one is better. >better vents. no smells. BUT also no humidity. we haven't been able to get >a ribbon when cutting since the move. so the perfect lab should have some >humidity. and how did you get a job where the pathologist only comes once >or twice a month?? >mb >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- No need to miss a message. Get email on-the-go with Yahoo! Mail for Mobile. Get started. From jmahoney <@t> alegent.org Fri Mar 16 13:44:34 2007 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Fri Mar 16 13:45:04 2007 Subject: [Histonet] Pre-labeling glass slides In-Reply-To: <923477.1710.qm@web61222.mail.yahoo.com> References: <923477.1710.qm@web61222.mail.yahoo.com> Message-ID: <45FA9F420200003C000085E1@gwia.alegent.org> Well Rene, I am and have been a Histo tech for over 20 years. I have been willing to change when I see things that will benefit the patient. I am a LEAN trainer and have implemented the LEAN principles in several labs. I did the DEEP STUDY in both LEAN and in Histology workflow. I can prove that LEAN will reduced TAT and errors while maintaining and even improving quality. To assume that LEAN will not work and that the principles are not applicable tells me that you do not know about the best part of LEAN and that is in the flexibility of it. There may be some "batching" but the smaller and less frequent the better. We still overnight process. In LEAN, when you are changing the "form, fit or function" of the specimen you are adding value to it. If something, like tissue processing, is necessary and cannot be eliminated could it be shortened or done in more frequent smaller "batches"? It is Friday afternoon and I will stop. When I get on a roll about LEAN I cannot stop because I have seen the benefits in our lab and in others. Obviously I am a believer and I love to talk about it. Rene, I hope we can meet sometime and continue this discussion in person. I always appreciate your insights on the histonet. Jan >>> Rene J Buesa 03/16/2007 11:23 AM >>> As it hapened with Janice I can't keep quiet either. Lets talk about LEAN. To start with it is not applicable to the entire histology workflow. The specimen arrives and somebody takes "hold" of it, number, access, describe AND??? that person will have to process it (unless it is a Frozen section). There has to be a delay and don't tell about the Xpress Sakura, it will have to wait until 30 or 40 cassettes will be ready before starting (and what do you call that if you don't want to call it a "batch"?) It will come out after 120 minutes (or more time if it is a regular processor) and again another person will take the cassette in a "nurturing and loving embrace" and will prepare the slide, embed the tissue, section it, WAIT until it dries and will stain it. How long will it take to that "dedicated" histotech to have the slide ready for the pathologist and, most important, how much did that slide cost? What happened to the other cases? Is it a "one specimen a day"lab? Trying to see the histology lab as the production line of a Toyota is unrealistic. Some aspects can be streamlined, others not. The only process that is LEAN (and has always been without knowing it by the way) is the one performed by the histotech rushing over the piece of tissue received from the OR to do a FS, and even there I have seen some mislabellings (like some labs that do not access the tissue to do the FS). Embracing new approaches require a deep study and knowing what we are getting into. Just my opinion (as always)! Ren? J. Douglas D Deltour wrote: So it will save us time by having the tech get up and print the slide (using the slide printer), go back to the microtome and cut it. This will happen about 300+ times a night. Let's put two techs into the equation and one will wait while the other is printing. If they do this I will not have to check the slides for accuracy anymore? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: Friday, March 16, 2007 10:25 AM To: Katri Tuomala; histonet@lists.utsouthwestern.edu; Carmen Leschuk; Rene J Buesa Subject: Re: [Histonet] Pre-labeling glass slides I can't keep quiet anymore. Single piece flow, one specimen, slide, case at a time is the fastest and least error prone way of performing any task. It is LEAN and it can be proven. Please consider it for the patient's sake. i know that it seems more efficient to batch. I felt that way too before I started seeing the processes in my lab with LEAN eyes. All those checks along the way are slowing down your process. If you single piece flow, one case/slide at at time you will make fewer errors and no longer need so many checks. You will get more slides out faster (with the right number on them whether they are written or etched or labelled). Jan Omaha Janice Mahoney Histology/Cytology Coordinator Alegent Health Laboratory 4955 F Street Omaha, NE 68117 (402)717-2889 >>> "Katri Tuomala" 03/12/2007 9:35 PM >>> I totally agree with Rene. We pre-label all our slides in approximately 20 slide lots. We have so many checks along the way, before the slides are delivered, that the odd mistake gets discovered very quickly. You just have to pay attention to what you are doing. And yes, I remember the diamond pens, ouch!! Katri Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "Rene J Buesa" To: "Carmen Leschuk" ; Sent: Monday, March 12, 2007 12:44 PM Subject: Re: [Histonet] Pre-labeling glass slides > Carmen: > Labelling the slides BEFORE they are used is the normal and logical > practice. In this way the slides can be ready beforehand. The thing is > that if the person using the slides does NOT pay attention, the > "misslabelling" can occur. > On the other hand the amount of time you could WASTE by leballing > simultaneously while sectioning is astronomical. > The good practice is to have a log with the blocks to section, prepare > all the labelled slides in the needed amount and BEFORE sectioning the > blocks, match slides to blocks and that is all you have to do. > Sloppines and carelessnes are the roots of any mistake in histology. The > solution is to pay attention, not to misuse a slides writing machine. > Ren? J. > > Carmen Leschuk wrote: > Recently, I heard of a slide mislabeling that occurred in another histo > lab that they determined to be caused by pre-labeling slides before actual > use at the microtome. This lab said that if the slides would of been > labeled simultaneously (per their policy) as the blocks being cut, the > mislabeling would of been prevented. My lab currently pre-labels all of > slides before cutting a sequence of 10-20 blocks, which I thought was > common practice. My question is, what is common practice? Do other labs > have poli > cies forbidding pre-labeling of glass slides? > > > > Carmen Leschuk, HT, SLS (ASCP) > Supervisor, SJMO-Anatomic Pathology > (248)858-6231 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Looking for earth-friendly autos? > Browse Top Cars by "Green Rating" at Yahoo! Autos' Green Center. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss an email again! Yahoo! Toolbar alerts you the instant new Mail arrives. Check it out. From rjbuesa <@t> yahoo.com Fri Mar 16 13:48:29 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Mar 16 13:48:39 2007 Subject: [Histonet] Pre-labeling glass slides In-Reply-To: <45FA81AE0200003C00008593@gwia.alegent.org> Message-ID: <339727.27089.qm@web61211.mail.yahoo.com> That is NOT a LEAN approach, that is old fashioned streamlining and trying to eliminate waste time and idling of personnel. I am beginning to think that you have heard about Lean but are not really aware of what it means, how is implemented and what areas are not completely suitable for it (like the histology workflow) in its fundamental functional factory approach. Rearanching the workflow to reduce the time the specimens idle, reduce the time the specimens are "left to their own" without being "touched" or incorprated into the work flow has always been an objective of streamlining that has been now "disguised" as Lean. Ren? J. Janice Mahoney wrote: I'm just saying that there are ways around this process. Consider things like moving your labeler closer to the cutting station for instance, or having someone other than a HT making and delivering the slides or maybe getting several slide labelers and putting them by the cutting stations. I totally agree that having tech get up and walk to another place to get slides is not efficient and having people standing and waiting to do work is even worse. I'm just saying that there are probably ways to make your work flow better and more efficiently by using LEAN principles. It is too complex to go into in an email but I encourage you to do some investigating and reading on the subject and you may discover some good ideas. Jan >>> "Douglas D Deltour" 03/16/2007 11:40 AM >>> So it will save us time by having the tech get up and print the slide (using the slide printer), go back to the microtome and cut it. This will happen about 300+ times a night. Let's put two techs into the equation and one will wait while the other is printing. If they do this I will not have to check the slides for accuracy anymore? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: Friday, March 16, 2007 10:25 AM To: Katri Tuomala; histonet@lists.utsouthwestern.edu; Carmen Leschuk; Rene J Buesa Subject: Re: [Histonet] Pre-labeling glass slides I can't keep quiet anymore. Single piece flow, one specimen, slide, case at a time is the fastest and least error prone way of performing any task. It is LEAN and it can be proven. Please consider it for the patient's sake. i know that it seems more efficient to batch. I felt that way too before I started seeing the processes in my lab with LEAN eyes. All those checks along the way are slowing down your process. If you single piece flow, one case/slide at at time you will make fewer errors and no longer need so many checks. You will get more slides out faster (with the right number on them whether they are written or etched or labelled). Jan Omaha Janice Mahoney Histology/Cytology Coordinator Alegent Health Laboratory 4955 F Street Omaha, NE 68117 (402)717-2889 >>> "Katri Tuomala" 03/12/2007 9:35 PM >>> I totally agree with Rene. We pre-label all our slides in approximately 20 slide lots. We have so many checks along the way, before the slides are delivered, that the odd mistake gets discovered very quickly. You just have to pay attention to what you are doing. And yes, I remember the diamond pens, ouch!! Katri Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "Rene J Buesa" To: "Carmen Leschuk" ; Sent: Monday, March 12, 2007 12:44 PM Subject: Re: [Histonet] Pre-labeling glass slides > Carmen: > Labelling the slides BEFORE they are used is the normal and logical > practice. In this way the slides can be ready beforehand. The thing is > that if the person using the slides does NOT pay attention, the > "misslabelling" can occur. > On the other hand the amount of time you could WASTE by leballing > simultaneously while sectioning is astronomical. > The good practice is to have a log with the blocks to section, prepare > all the labelled slides in the needed amount and BEFORE sectioning the > blocks, match slides to blocks and that is all you have to do. > Sloppines and carelessnes are the roots of any mistake in histology. The > solution is to pay attention, not to misuse a slides writing machine. > Ren? J. > > Carmen Leschuk wrote: > Recently, I heard of a slide mislabeling that occurred in another histo > lab that they determined to be caused by pre-labeling slides before actual > use at the microtome. This lab said that if the slides would of been > labeled simultaneously (per their policy) as the blocks being cut, the > mislabeling would of been prevented. My lab currently pre-labels all of > slides before cutting a sequence of 10-20 blocks, which I thought was > common practice. My question is, what is common practice? Do other labs > have poli > cies forbidding pre-labeling of glass slides? > > > > Carmen Leschuk, HT, SLS (ASCP) > Supervisor, SJMO-Anatomic Pathology > (248)858-6231 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Looking for earth-friendly autos? > Browse Top Cars by "Green Rating" at Yahoo! Autos' Green Center. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Don't get soaked. Take a quick peek at the forecast with theYahoo! Search weather shortcut. From doug <@t> ppspath.com Fri Mar 16 14:52:02 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Fri Mar 16 13:51:24 2007 Subject: [Histonet] Pre-labeling glass slides In-Reply-To: <45FA9F420200003C000085E1@gwia.alegent.org> Message-ID: Jan, How flexible can it be before it is not considered LEAN? Also I am curious of your type of facility and workload. Thanks Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: Janice Mahoney [mailto:jmahoney@alegent.org] Sent: Friday, March 16, 2007 1:45 PM To: 'Katri Tuomala'; histonet@lists.utsouthwestern.edu; Douglas D Deltour; 'Carmen Leschuk'; Rene J Buesa Subject: RE: [Histonet] Pre-labeling glass slides Well Rene, I am and have been a Histo tech for over 20 years. I have been willing to change when I see things that will benefit the patient. I am a LEAN trainer and have implemented the LEAN principles in several labs. I did the DEEP STUDY in both LEAN and in Histology workflow. I can prove that LEAN will reduced TAT and errors while maintaining and even improving quality. To assume that LEAN will not work and that the principles are not applicable tells me that you do not know about the best part of LEAN and that is in the flexibility of it. There may be some "batching" but the smaller and less frequent the better. We still overnight process. In LEAN, when you are changing the "form, fit or function" of the specimen you are adding value to it. If something, like tissue processing, is necessary and cannot be eliminated could it be shortened or done in more frequent smaller "batches"? It is Friday afternoon and I will stop. When I get on a roll about LEAN I cannot stop because I have seen the benefits in our lab and in others. Obviously I am a believer and I love to talk about it. Rene, I hope we can meet sometime and continue this discussion in person. I always appreciate your insights on the histonet. Jan >>> Rene J Buesa 03/16/2007 11:23 AM >>> As it hapened with Janice I can't keep quiet either. Lets talk about LEAN. To start with it is not applicable to the entire histology workflow. The specimen arrives and somebody takes "hold" of it, number, access, describe AND??? that person will have to process it (unless it is a Frozen section). There has to be a delay and don't tell about the Xpress Sakura, it will have to wait until 30 or 40 cassettes will be ready before starting (and what do you call that if you don't want to call it a "batch"?) It will come out after 120 minutes (or more time if it is a regular processor) and again another person will take the cassette in a "nurturing and loving embrace" and will prepare the slide, embed the tissue, section it, WAIT until it dries and will stain it. How long will it take to that "dedicated" histotech to have the slide ready for the pathologist and, most important, how much did that slide cost? What happened to the other cases? Is it a "one specimen a day"lab? Trying to see the histology lab as the production line of a Toyota is unrealistic. Some aspects can be streamlined, others not. The only process that is LEAN (and has always been without knowing it by the way) is the one performed by the histotech rushing over the piece of tissue received from the OR to do a FS, and even there I have seen some mislabellings (like some labs that do not access the tissue to do the FS). Embracing new approaches require a deep study and knowing what we are getting into. Just my opinion (as always)! Ren? J. Douglas D Deltour wrote: So it will save us time by having the tech get up and print the slide (using the slide printer), go back to the microtome and cut it. This will happen about 300+ times a night. Let's put two techs into the equation and one will wait while the other is printing. If they do this I will not have to check the slides for accuracy anymore? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: Friday, March 16, 2007 10:25 AM To: Katri Tuomala; histonet@lists.utsouthwestern.edu; Carmen Leschuk; Rene J Buesa Subject: Re: [Histonet] Pre-labeling glass slides I can't keep quiet anymore. Single piece flow, one specimen, slide, case at a time is the fastest and least error prone way of performing any task. It is LEAN and it can be proven. Please consider it for the patient's sake. i know that it seems more efficient to batch. I felt that way too before I started seeing the processes in my lab with LEAN eyes. All those checks along the way are slowing down your process. If you single piece flow, one case/slide at at time you will make fewer errors and no longer need so many checks. You will get more slides out faster (with the right number on them whether they are written or etched or labelled). Jan Omaha Janice Mahoney Histology/Cytology Coordinator Alegent Health Laboratory 4955 F Street Omaha, NE 68117 (402)717-2889 >>> "Katri Tuomala" 03/12/2007 9:35 PM >>> I totally agree with Rene. We pre-label all our slides in approximately 20 slide lots. We have so many checks along the way, before the slides are delivered, that the odd mistake gets discovered very quickly. You just have to pay attention to what you are doing. And yes, I remember the diamond pens, ouch!! Katri Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "Rene J Buesa" To: "Carmen Leschuk" ; Sent: Monday, March 12, 2007 12:44 PM Subject: Re: [Histonet] Pre-labeling glass slides > Carmen: > Labelling the slides BEFORE they are used is the normal and logical > practice. In this way the slides can be ready beforehand. The thing is > that if the person using the slides does NOT pay attention, the > "misslabelling" can occur. > On the other hand the amount of time you could WASTE by leballing > simultaneously while sectioning is astronomical. > The good practice is to have a log with the blocks to section, prepare > all the labelled slides in the needed amount and BEFORE sectioning the > blocks, match slides to blocks and that is all you have to do. > Sloppines and carelessnes are the roots of any mistake in histology. The > solution is to pay attention, not to misuse a slides writing machine. > Ren? J. > > Carmen Leschuk wrote: > Recently, I heard of a slide mislabeling that occurred in another histo > lab that they determined to be caused by pre-labeling slides before actual > use at the microtome. This lab said that if the slides would of been > labeled simultaneously (per their policy) as the blocks being cut, the > mislabeling would of been prevented. My lab currently pre-labels all of > slides before cutting a sequence of 10-20 blocks, which I thought was > common practice. My question is, what is common practice? Do other labs > have poli > cies forbidding pre-labeling of glass slides? > > > > Carmen Leschuk, HT, SLS (ASCP) > Supervisor, SJMO-Anatomic Pathology > (248)858-6231 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Looking for earth-friendly autos? > Browse Top Cars by "Green Rating" at Yahoo! Autos' Green Center. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss an email again! Yahoo! Toolbar alerts you the instant new Mail arrives. Check it out. From rjbuesa <@t> yahoo.com Fri Mar 16 14:00:34 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Mar 16 14:00:44 2007 Subject: [Histonet] Pre-labeling glass slides In-Reply-To: <45FA9F420200003C000085E1@gwia.alegent.org> Message-ID: <660702.31747.qm@web61211.mail.yahoo.com> OK, now you are talking about the "dreaded" work, the "B" word, the BATCH that if one wants to be efficient has to be small and dealt with as soon as possible. What you are writing about is a DIVISION of the workflow into several small batches or subunits to be dealt with effciciently and quickly. That is NOT LEAN, that is common sense streamlining, as well as the ubication of the different stations to reflect the sequence in the work flow. I did that in all the labs I have managed since before the people began talking about Lean. Lean is a concept, a managerial idea whose GENERAL principles can be adapted to histology but whose initial factory approach of almost zero inventory and productivity program are essentially incompatible with the histology flow that is completely dependant on the time the tissue is processed that in its turn is indepandent of all the pre-processing and post-processing tasks. Even the "elleged" Lean tissue processor by Sakura (the Xpress) promoted as "lean" needs the preparation of 30 to 40 cassettes batches requiring more time of pre and post-processing time than the real time dedicated to process (120 minutes if fixation is done previuously). Altough old and with 54 years of experience, I have always been open to new technologies. I consider myself as a fosil with a juvenile mind! Ren? J. Janice Mahoney wrote: Well Rene, I am and have been a Histo tech for over 20 years. I have been willing to change when I see things that will benefit the patient. I am a LEAN trainer and have implemented the LEAN principles in several labs. I did the DEEP STUDY in both LEAN and in Histology workflow. I can prove that LEAN will reduced TAT and errors while maintaining and even improving quality. To assume that LEAN will not work and that the principles are not applicable tells me that you do not know about the best part of LEAN and that is in the flexibility of it. There may be some "batching" but the smaller and less frequent the better. We still overnight process. In LEAN, when you are changing the "form, fit or function" of the specimen you are adding value to it. If something, like tissue processing, is necessary and cannot be eliminated could it be shortened or done in more frequent smaller "batches"? It is Friday afternoon and I will stop. When I get on a roll about LEAN I cannot stop because I have seen the benefits in our lab and in others. Obviously I am a believer and I love to talk about it. Rene, I hope we can meet sometime and continue this discussion in person. I always appreciate your insights on the histonet. Jan >>> Rene J Buesa 03/16/2007 11:23 AM >>> As it hapened with Janice I can't keep quiet either. Lets talk about LEAN. To start with it is not applicable to the entire histology workflow. The specimen arrives and somebody takes "hold" of it, number, access, describe AND??? that person will have to process it (unless it is a Frozen section). There has to be a delay and don't tell about the Xpress Sakura, it will have to wait until 30 or 40 cassettes will be ready before starting (and what do you call that if you don't want to call it a "batch"?) It will come out after 120 minutes (or more time if it is a regular processor) and again another person will take the cassette in a "nurturing and loving embrace" and will prepare the slide, embed the tissue, section it, WAIT until it dries and will stain it. How long will it take to that "dedicated" histotech to have the slide ready for the pathologist and, most important, how much did that slide cost? What happened to the other cases? Is it a "one specimen a day"lab? Trying to see the histology lab as the production line of a Toyota is unrealistic. Some aspects can be streamlined, others not. The only process that is LEAN (and has always been without knowing it by the way) is the one performed by the histotech rushing over the piece of tissue received from the OR to do a FS, and even there I have seen some mislabellings (like some labs that do not access the tissue to do the FS). Embracing new approaches require a deep study and knowing what we are getting into. Just my opinion (as always)! Ren? J. Douglas D Deltour wrote: So it will save us time by having the tech get up and print the slide (using the slide printer), go back to the microtome and cut it. This will happen about 300+ times a night. Let's put two techs into the equation and one will wait while the other is printing. If they do this I will not have to check the slides for accuracy anymore? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: Friday, March 16, 2007 10:25 AM To: Katri Tuomala; histonet@lists.utsouthwestern.edu; Carmen Leschuk; Rene J Buesa Subject: Re: [Histonet] Pre-labeling glass slides I can't keep quiet anymore. Single piece flow, one specimen, slide, case at a time is the fastest and least error prone way of performing any task. It is LEAN and it can be proven. Please consider it for the patient's sake. i know that it seems more efficient to batch. I felt that way too before I started seeing the processes in my lab with LEAN eyes. All those checks along the way are slowing down your process. If you single piece flow, one case/slide at at time you will make fewer errors and no longer need so many checks. You will get more slides out faster (with the right number on them whether they are written or etched or labelled). Jan Omaha Janice Mahoney Histology/Cytology Coordinator Alegent Health Laboratory 4955 F Street Omaha, NE 68117 (402)717-2889 >>> "Katri Tuomala" 03/12/2007 9:35 PM >>> I totally agree with Rene. We pre-label all our slides in approximately 20 slide lots. We have so many checks along the way, before the slides are delivered, that the odd mistake gets discovered very quickly. You just have to pay attention to what you are doing. And yes, I remember the diamond pens, ouch!! Katri Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "Rene J Buesa" To: "Carmen Leschuk" ; Sent: Monday, March 12, 2007 12:44 PM Subject: Re: [Histonet] Pre-labeling glass slides > Carmen: > Labelling the slides BEFORE they are used is the normal and logical > practice. In this way the slides can be ready beforehand. The thing is > that if the person using the slides does NOT pay attention, the > "misslabelling" can occur. > On the other hand the amount of time you could WASTE by leballing > simultaneously while sectioning is astronomical. > The good practice is to have a log with the blocks to section, prepare > all the labelled slides in the needed amount and BEFORE sectioning the > blocks, match slides to blocks and that is all you have to do. > Sloppines and carelessnes are the roots of any mistake in histology. The > solution is to pay attention, not to misuse a slides writing machine. > Ren? J. > > Carmen Leschuk wrote: > Recently, I heard of a slide mislabeling that occurred in another histo > lab that they determined to be caused by pre-labeling slides before actual > use at the microtome. This lab said that if the slides would of been > labeled simultaneously (per their policy) as the blocks being cut, the > mislabeling would of been prevented. My lab currently pre-labels all of > slides before cutting a sequence of 10-20 blocks, which I thought was > common practice. My question is, what is common practice? Do other labs > have poli > cies forbidding pre-labeling of glass slides? > > > > Carmen Leschuk, HT, SLS (ASCP) > Supervisor, SJMO-Anatomic Pathology > (248)858-6231 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Looking for earth-friendly autos? > Browse Top Cars by "Green Rating" at Yahoo! Autos' Green Center. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss an email again! Yahoo! Toolbar alerts you the instant new Mail arrives. Check it out. --------------------------------- Need Mail bonding? Go to the Yahoo! Mail Q&A for great tips from Yahoo! Answers users. From rjbuesa <@t> yahoo.com Fri Mar 16 14:00:37 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Mar 16 14:00:45 2007 Subject: [Histonet] Pre-labeling glass slides In-Reply-To: <45FA9F420200003C000085E1@gwia.alegent.org> Message-ID: <108560.3411.qm@web61223.mail.yahoo.com> OK, now you are talking about the "dreaded" work, the "B" word, the BATCH that if one wants to be efficient has to be small and dealt with as soon as possible. What you are writing about is a DIVISION of the workflow into several small batches or subunits to be dealt with effciciently and quickly. That is NOT LEAN, that is common sense streamlining, as well as the ubication of the different stations to reflect the sequence in the work flow. I did that in all the labs I have managed since before the people began talking about Lean. Lean is a concept, a managerial idea whose GENERAL principles can be adapted to histology but whose initial factory approach of almost zero inventory and productivity program are essentially incompatible with the histology flow that is completely dependant on the time the tissue is processed that in its turn is indepandent of all the pre-processing and post-processing tasks. Even the "elleged" Lean tissue processor by Sakura (the Xpress) promoted as "lean" needs the preparation of 30 to 40 cassettes batches requiring more time of pre and post-processing time than the real time dedicated to process (120 minutes if fixation is done previuously). Altough old and with 54 years of experience, I have always been open to new technologies. I consider myself as a fosil with a juvenile mind! Ren? J. Janice Mahoney wrote: Well Rene, I am and have been a Histo tech for over 20 years. I have been willing to change when I see things that will benefit the patient. I am a LEAN trainer and have implemented the LEAN principles in several labs. I did the DEEP STUDY in both LEAN and in Histology workflow. I can prove that LEAN will reduced TAT and errors while maintaining and even improving quality. To assume that LEAN will not work and that the principles are not applicable tells me that you do not know about the best part of LEAN and that is in the flexibility of it. There may be some "batching" but the smaller and less frequent the better. We still overnight process. In LEAN, when you are changing the "form, fit or function" of the specimen you are adding value to it. If something, like tissue processing, is necessary and cannot be eliminated could it be shortened or done in more frequent smaller "batches"? It is Friday afternoon and I will stop. When I get on a roll about LEAN I cannot stop because I have seen the benefits in our lab and in others. Obviously I am a believer and I love to talk about it. Rene, I hope we can meet sometime and continue this discussion in person. I always appreciate your insights on the histonet. Jan >>> Rene J Buesa 03/16/2007 11:23 AM >>> As it hapened with Janice I can't keep quiet either. Lets talk about LEAN. To start with it is not applicable to the entire histology workflow. The specimen arrives and somebody takes "hold" of it, number, access, describe AND??? that person will have to process it (unless it is a Frozen section). There has to be a delay and don't tell about the Xpress Sakura, it will have to wait until 30 or 40 cassettes will be ready before starting (and what do you call that if you don't want to call it a "batch"?) It will come out after 120 minutes (or more time if it is a regular processor) and again another person will take the cassette in a "nurturing and loving embrace" and will prepare the slide, embed the tissue, section it, WAIT until it dries and will stain it. How long will it take to that "dedicated" histotech to have the slide ready for the pathologist and, most important, how much did that slide cost? What happened to the other cases? Is it a "one specimen a day"lab? Trying to see the histology lab as the production line of a Toyota is unrealistic. Some aspects can be streamlined, others not. The only process that is LEAN (and has always been without knowing it by the way) is the one performed by the histotech rushing over the piece of tissue received from the OR to do a FS, and even there I have seen some mislabellings (like some labs that do not access the tissue to do the FS). Embracing new approaches require a deep study and knowing what we are getting into. Just my opinion (as always)! Ren? J. Douglas D Deltour wrote: So it will save us time by having the tech get up and print the slide (using the slide printer), go back to the microtome and cut it. This will happen about 300+ times a night. Let's put two techs into the equation and one will wait while the other is printing. If they do this I will not have to check the slides for accuracy anymore? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: Friday, March 16, 2007 10:25 AM To: Katri Tuomala; histonet@lists.utsouthwestern.edu; Carmen Leschuk; Rene J Buesa Subject: Re: [Histonet] Pre-labeling glass slides I can't keep quiet anymore. Single piece flow, one specimen, slide, case at a time is the fastest and least error prone way of performing any task. It is LEAN and it can be proven. Please consider it for the patient's sake. i know that it seems more efficient to batch. I felt that way too before I started seeing the processes in my lab with LEAN eyes. All those checks along the way are slowing down your process. If you single piece flow, one case/slide at at time you will make fewer errors and no longer need so many checks. You will get more slides out faster (with the right number on them whether they are written or etched or labelled). Jan Omaha Janice Mahoney Histology/Cytology Coordinator Alegent Health Laboratory 4955 F Street Omaha, NE 68117 (402)717-2889 >>> "Katri Tuomala" 03/12/2007 9:35 PM >>> I totally agree with Rene. We pre-label all our slides in approximately 20 slide lots. We have so many checks along the way, before the slides are delivered, that the odd mistake gets discovered very quickly. You just have to pay attention to what you are doing. And yes, I remember the diamond pens, ouch!! Katri Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "Rene J Buesa" To: "Carmen Leschuk" ; Sent: Monday, March 12, 2007 12:44 PM Subject: Re: [Histonet] Pre-labeling glass slides > Carmen: > Labelling the slides BEFORE they are used is the normal and logical > practice. In this way the slides can be ready beforehand. The thing is > that if the person using the slides does NOT pay attention, the > "misslabelling" can occur. > On the other hand the amount of time you could WASTE by leballing > simultaneously while sectioning is astronomical. > The good practice is to have a log with the blocks to section, prepare > all the labelled slides in the needed amount and BEFORE sectioning the > blocks, match slides to blocks and that is all you have to do. > Sloppines and carelessnes are the roots of any mistake in histology. The > solution is to pay attention, not to misuse a slides writing machine. > Ren? J. > > Carmen Leschuk wrote: > Recently, I heard of a slide mislabeling that occurred in another histo > lab that they determined to be caused by pre-labeling slides before actual > use at the microtome. This lab said that if the slides would of been > labeled simultaneously (per their policy) as the blocks being cut, the > mislabeling would of been prevented. My lab currently pre-labels all of > slides before cutting a sequence of 10-20 blocks, which I thought was > common practice. My question is, what is common practice? Do other labs > have poli > cies forbidding pre-labeling of glass slides? > > > > Carmen Leschuk, HT, SLS (ASCP) > Supervisor, SJMO-Anatomic Pathology > (248)858-6231 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Looking for earth-friendly autos? > Browse Top Cars by "Green Rating" at Yahoo! Autos' Green Center. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss an email again! Yahoo! Toolbar alerts you the instant new Mail arrives. Check it out. --------------------------------- Now that's room service! Choose from over 150,000 hotels in 45,000 destinations on Yahoo! Travel to find your fit. From GMartin <@t> marshallhospital.org Fri Mar 16 14:06:33 2007 From: GMartin <@t> marshallhospital.org (GMartin@marshallhospital.org) Date: Fri Mar 16 14:03:52 2007 Subject: [Histonet] Pre-labeling Message-ID: Thank you Janice for speaking up. I agree with you NOT to pre-label. I work next to a histo tec that has "been doing this forever" and watch every morning while she numbers slides. At the same time I am cutting slides and providing immediate patient care. My batch is on the warmer at least 15 minutes before she even has a slide cut ... and guess what ... when I look at the error log ... guess who has the number errors ... it's not me! The same thing goes for the pathologist that cut in ... one of our pathologist insists one pulling all the requisitions away from the stocked specimens and pre-numbers the blocks ... guess who makes the most number mistakes ... it's not the pathologists that numbers as he goes. From GMartin <@t> marshallhospital.org Fri Mar 16 14:12:08 2007 From: GMartin <@t> marshallhospital.org (GMartin@marshallhospital.org) Date: Fri Mar 16 14:09:27 2007 Subject: [Histonet] Pre-labeling Message-ID: I realized after I wrote my response to the labeling issue, that some of us still hand label. It seems obvious if your automated that batch labeling is probably the only thing to do! Gary From rjbuesa <@t> yahoo.com Fri Mar 16 14:17:07 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Mar 16 14:17:14 2007 Subject: [Histonet] Pre-labeling In-Reply-To: Message-ID: <626828.52437.qm@web61220.mail.yahoo.com> You have described 2 underachiers and poor performers that cannot be considered (hopefully) as "the norm". I have also known of those who write the slide when the section is already in it, and you know what, they have also made mistakes. The thing is not how do you do things, but how much attention you pay to what you do. From the productivity point of view is much more efficient to have all the slides written and matched by NON licensed personnel (lab aides) and given already matched to the HT that are sectioning, than use valuable (and costly) time to do the same thing either pre- or post sectioning. Ren? J. GMartin@marshallhospital.org wrote: Thank you Janice for speaking up. I agree with you NOT to pre-label. I work next to a histo tec that has "been doing this forever" and watch every morning while she numbers slides. At the same time I am cutting slides and providing immediate patient care. My batch is on the warmer at least 15 minutes before she even has a slide cut ... and guess what ... when I look at the error log ... guess who has the number errors ... it's not me! The same thing goes for the pathologist that cut in ... one of our pathologist insists one pulling all the requisitions away from the stocked specimens and pre-numbers the blocks ... guess who makes the most number mistakes ... it's not the pathologists that numbers as he goes. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- We won't tell. Get more on shows you hate to love (and love to hate): Yahoo! TV's Guilty Pleasures list. From Heather.D.Renko <@t> osfhealthcare.org Fri Mar 16 14:26:48 2007 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Fri Mar 16 14:27:05 2007 Subject: [Histonet] SLIDE PRE-LABELING Message-ID: <40026EDDE64CDA47AB382C52619ACD3C06612CB9@pmc-rfd-mx01.intranet.osfnet.org> Additionally, I agree with Ren? and we too pre label our slides/20 at a time and it works very well for our facility. Which is the key statement here! You must set up many road blocks to prevent error, whether its before you cut the slide or after and there are many that can share how they do this. We as a department pay very close attention to the number on the block when we put it on the microtome as one of many checks. Then as you are putting your twenty slides into the staining rack we then cross reference them to the block order, we then lay them out and check blocks after they are stained. For us this works very well with little error. For other labs this might create error! I prefer this method of slide preparation to labeling them one by one. If there are too many errors with one individual then maybe it is an attention problem and maybe that might be addressed. There is no one way to do anything in a histology lab, we just simply need to develop the system/flow that works best for the department and for the techs doing the slide preparation. When I look at flow and procedure; accurate, timely patient care is my only concern. Happy Friday!! Heather Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From rjbuesa <@t> yahoo.com Fri Mar 16 14:27:56 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Mar 16 14:28:04 2007 Subject: [Histonet] Pre-labeling In-Reply-To: Message-ID: <364684.66151.qm@web61217.mail.yahoo.com> No, it is not the only thing because if you do not pay attention to what you do you could end using the wrong pre-written slide for the wrong section. No automated step or instrument if "fool proof" and avoiding to being a "fool" is what is all about! Ren? J. GMartin@marshallhospital.org wrote: I realized after I wrote my response to the labeling issue, that some of us still hand label. It seems obvious if your automated that batch labeling is probably the only thing to do! Gary _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- The fish are biting. Get more visitors on your site using Yahoo! Search Marketing. From carl.hobbs <@t> kcl.ac.uk Fri Mar 16 14:38:46 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Fri Mar 16 14:39:12 2007 Subject: [Histonet] Re: antibody 3A10 Message-ID: <001201c76802$b6a7ebf0$4101a8c0@carlba65530bda> Hi. I have sent a PM to you. In addition, I am not really satisfied with 3A10.....I can often get "better" results using the various anti NF ABs. One has to "play" with various Abs, IMHO, to get the optimal signal. Good luck! Carl From doug <@t> ppspath.com Fri Mar 16 15:42:31 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Fri Mar 16 14:40:21 2007 Subject: [Histonet] SLIDE PRE-LABELING In-Reply-To: <40026EDDE64CDA47AB382C52619ACD3C06612CB9@pmc-rfd-mx01.intranet.osfnet.org> Message-ID: If we had the luxury of time we would label our slides as we go but we are on timelines and deadlines. I print up the slides from the grossing sheet during the day and the work is cut at night/early morning. For the average hospital it may work out fine to label as you go but if you are doing 500+ slides a night it is another story. Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Renko, Heather D. Sent: Friday, March 16, 2007 2:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] SLIDE PRE-LABELING Additionally, I agree with Ren? and we too pre label our slides/20 at a time and it works very well for our facility. Which is the key statement here! You must set up many road blocks to prevent error, whether its before you cut the slide or after and there are many that can share how they do this. We as a department pay very close attention to the number on the block when we put it on the microtome as one of many checks. Then as you are putting your twenty slides into the staining rack we then cross reference them to the block order, we then lay them out and check blocks after they are stained. For us this works very well with little error. For other labs this might create error! I prefer this method of slide preparation to labeling them one by one. If there are too many errors with one individual then maybe it is an attention problem and maybe that might be addressed. There is no one way to do anything in a histology lab, we just simply need to develop the system/flow that works best for the department and for the techs doing the slide preparation. When I look at flow and procedure; accurate, timely patient care is my only concern. Happy Friday!! Heather Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================ == The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================ == _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ROrr <@t> enh.org Fri Mar 16 14:48:28 2007 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Fri Mar 16 14:48:35 2007 Subject: [Histonet] Lean for Dummies Message-ID: Hey kids, I was shopping in the local bookstore and came across one of those "...for Dummies" books. This one was for LEAN and SIX Sigma. I opened it and started reading...then my eyes blurred and my head started spinning and I felt a burning sensation... I thought it was because I was reading about this new paradigm we are implementing, but I realized I had spilled my starbucks all over my lap. Happy Friday Who's ready for Green beer and donuts! Becky Orr CLA,HT(ASCP)QIHC Assistant Manager, Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 As it hapened with Janice I can't keep quiet either. Lets talk about LEAN. To start with it is not applicable to the entire histology workflow. The specimen arrives and somebody takes "hold" of it, number, access, describe AND??? that person will have to process it (unless it is a Frozen section). There has to be a delay and don't tell about the Xpress Sakura, it will have to wait until 30 or 40 cassettes will be ready before starting (and what do you call that if you don't want to call it a "batch"?) It will come out after 120 minutes (or more time if it is a regular processor) and again another person will take the cassette in a "nurturing and loving embrace" and will prepare the slide, embed the tissue, section it, WAIT until it dries and will stain it. How long will it take to that "dedicated" histotech to have the slide ready for the pathologist and, most important, how much did that slide cost? What happened to the other cases? Is it a "one specimen a day"lab? Trying to see the histology lab as the production line of a Toyota is unrealistic. Some aspects can be streamlined, others not. The only process that is LEAN (and has always been without knowing it by the way) is the one performed by the histotech rushing over the piece of tissue received from the OR to do a FS, and even there I have seen some mislabellings (like some labs that do not access the tissue to do the FS). Embracing new approaches require a deep study and knowing what we are getting into. Just my opinion (as always)! Ren? J. From jmahoney <@t> alegent.org Fri Mar 16 14:49:33 2007 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Fri Mar 16 14:50:11 2007 Subject: [Histonet] Pre-labeling glass slides In-Reply-To: <20070316185031.A2A257176EB@mail136-cpk.bigfish.com> References: <45FA9F420200003C000085E1@gwia.alegent.org> <20070316185031.A2A257176EB@mail136-cpk.bigfish.com> Message-ID: <45FAAE7D0200003C00008611@gwia.alegent.org> Hi Douglas, We are a clinical lab that services 7 hospitals and over 100 clinics in Nebraska and iowa. We do 38,000 surgical cases per year. Not very big by some standards but huge by others. There is flexibility when applying those 5 principles. I like it that applying LEAN to a laboratory is NOT the same as manufacturing. We don't have to be so stringent and we can still see positive results and still be LEAN. The whole manufacturing thing turns so many people off but it is only because they do not understand or have the experience of seeing this done in a laboratory setting. Jan >>> "Douglas D Deltour" 03/16/2007 2:52 PM >>> Jan, How flexible can it be before it is not considered LEAN? Also I am curious of your type of facility and workload. Thanks Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: Janice Mahoney [mailto:jmahoney@alegent.org] Sent: Friday, March 16, 2007 1:45 PM To: 'Katri Tuomala'; histonet@lists.utsouthwestern.edu; Douglas D Deltour; 'Carmen Leschuk'; Rene J Buesa Subject: RE: [Histonet] Pre-labeling glass slides Well Rene, I am and have been a Histo tech for over 20 years. I have been willing to change when I see things that will benefit the patient. I am a LEAN trainer and have implemented the LEAN principles in several labs. I did the DEEP STUDY in both LEAN and in Histology workflow. I can prove that LEAN will reduced TAT and errors while maintaining and even improving quality. To assume that LEAN will not work and that the principles are not applicable tells me that you do not know about the best part of LEAN and that is in the flexibility of it. There may be some "batching" but the smaller and less frequent the better. We still overnight process. In LEAN, when you are changing the "form, fit or function" of the specimen you are adding value to it. If something, like tissue processing, is necessary and cannot be eliminated could it be shortened or done in more frequent smaller "batches"? It is Friday afternoon and I will stop. When I get on a roll about LEAN I cannot stop because I have seen the benefits in our lab and in others. Obviously I am a believer and I love to talk about it. Rene, I hope we can meet sometime and continue this discussion in person. I always appreciate your insights on the histonet. Jan >>> Rene J Buesa 03/16/2007 11:23 AM >>> As it hapened with Janice I can't keep quiet either. Lets talk about LEAN. To start with it is not applicable to the entire histology workflow. The specimen arrives and somebody takes "hold" of it, number, access, describe AND??? that person will have to process it (unless it is a Frozen section). There has to be a delay and don't tell about the Xpress Sakura, it will have to wait until 30 or 40 cassettes will be ready before starting (and what do you call that if you don't want to call it a "batch"?) It will come out after 120 minutes (or more time if it is a regular processor) and again another person will take the cassette in a "nurturing and loving embrace" and will prepare the slide, embed the tissue, section it, WAIT until it dries and will stain it. How long will it take to that "dedicated" histotech to have the slide ready for the pathologist and, most important, how much did that slide cost? What happened to the other cases? Is it a "one specimen a day"lab? Trying to see the histology lab as the production line of a Toyota is unrealistic. Some aspects can be streamlined, others not. The only process that is LEAN (and has always been without knowing it by the way) is the one performed by the histotech rushing over the piece of tissue received from the OR to do a FS, and even there I have seen some mislabellings (like some labs that do not access the tissue to do the FS). Embracing new approaches require a deep study and knowing what we are getting into. Just my opinion (as always)! Ren? J. Douglas D Deltour wrote: So it will save us time by having the tech get up and print the slide (using the slide printer), go back to the microtome and cut it. This will happen about 300+ times a night. Let's put two techs into the equation and one will wait while the other is printing. If they do this I will not have to check the slides for accuracy anymore? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: Friday, March 16, 2007 10:25 AM To: Katri Tuomala; histonet@lists.utsouthwestern.edu; Carmen Leschuk; Rene J Buesa Subject: Re: [Histonet] Pre-labeling glass slides I can't keep quiet anymore. Single piece flow, one specimen, slide, case at a time is the fastest and least error prone way of performing any task. It is LEAN and it can be proven. Please consider it for the patient's sake. i know that it seems more efficient to batch. I felt that way too before I started seeing the processes in my lab with LEAN eyes. All those checks along the way are slowing down your process. If you single piece flow, one case/slide at at time you will make fewer errors and no longer need so many checks. You will get more slides out faster (with the right number on them whether they are written or etched or labelled). Jan Omaha Janice Mahoney Histology/Cytology Coordinator Alegent Health Laboratory 4955 F Street Omaha, NE 68117 (402)717-2889 >>> "Katri Tuomala" 03/12/2007 9:35 PM >>> I totally agree with Rene. We pre-label all our slides in approximately 20 slide lots. We have so many checks along the way, before the slides are delivered, that the odd mistake gets discovered very quickly. You just have to pay attention to what you are doing. And yes, I remember the diamond pens, ouch!! Katri Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "Rene J Buesa" To: "Carmen Leschuk" ; Sent: Monday, March 12, 2007 12:44 PM Subject: Re: [Histonet] Pre-labeling glass slides > Carmen: > Labelling the slides BEFORE they are used is the normal and logical > practice. In this way the slides can be ready beforehand. The thing is > that if the person using the slides does NOT pay attention, the > "misslabelling" can occur. > On the other hand the amount of time you could WASTE by leballing > simultaneously while sectioning is astronomical. > The good practice is to have a log with the blocks to section, prepare > all the labelled slides in the needed amount and BEFORE sectioning the > blocks, match slides to blocks and that is all you have to do. > Sloppines and carelessnes are the roots of any mistake in histology. The > solution is to pay attention, not to misuse a slides writing machine. > Ren? J. > > Carmen Leschuk wrote: > Recently, I heard of a slide mislabeling that occurred in another histo > lab that they determined to be caused by pre-labeling slides before actual > use at the microtome. This lab said that if the slides would of been > labeled simultaneously (per their policy) as the blocks being cut, the > mislabeling would of been prevented. My lab currently pre-labels all of > slides before cutting a sequence of 10-20 blocks, which I thought was > common practice. My question is, what is common practice? Do other labs > have poli > cies forbidding pre-labeling of glass slides? > > > > Carmen Leschuk, HT, SLS (ASCP) > Supervisor, SJMO-Anatomic Pathology > (248)858-6231 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Looking for earth-friendly autos? > Browse Top Cars by "Green Rating" at Yahoo! Autos' Green Center. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss an email again! Yahoo! Toolbar alerts you the instant new Mail arrives. Check it out. From atebo <@t> aahs.org Fri Mar 16 14:54:16 2007 From: atebo <@t> aahs.org (Tebo, Andrea) Date: Fri Mar 16 14:54:35 2007 Subject: [Histonet] SLIDE PRE-LABELING In-Reply-To: <40026EDDE64CDA47AB382C52619ACD3C06612CB9@pmc-rfd-mx01.intranet.osfnet.org> References: <40026EDDE64CDA47AB382C52619ACD3C06612CB9@pmc-rfd-mx01.intranet.osfnet.org> Message-ID: <00790F10D0600A41AEE8899901E533A61044B57D@aamcexch.aamc.org> I also agree with the prelabling. The Gross Room Tech actually labels all of our slides the night before so when the techs arrive at 5:00am, they can begin embedding and cutting. We separate them by the number of slides per block and they are in numerical order. Our cassettes are color coded, green = 3 slides, white = 1 slide, Lilac = 6 slides. If the tech is cutting the green cassettes, they have the box marked X 3 on the side. The blocks are cut in numerical order, so their slides should go along with their blocks. Once in a while, a group of slides is missed, it is the person's responsibility to make sure that the blocks match the slides before they cut the block. It works very well for us. We have 3 people cutting 200 blocks, we do not have time to write as we go. If we did, the doctors would not receive the bulk of their slides until late afternoon. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Renko, Heather D. Sent: Friday, March 16, 2007 3:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] SLIDE PRE-LABELING Additionally, I agree with Ren? and we too pre label our slides/20 at a time and it works very well for our facility. Which is the key statement here! You must set up many road blocks to prevent error, whether its before you cut the slide or after and there are many that can share how they do this. We as a department pay very close attention to the number on the block when we put it on the microtome as one of many checks. Then as you are putting your twenty slides into the staining rack we then cross reference them to the block order, we then lay them out and check blocks after they are stained. For us this works very well with little error. For other labs this might create error! I prefer this method of slide preparation to labeling them one by one. If there are too many errors with one individual then maybe it is an attention problem and maybe that might be addressed. There is no one way to do anything in a histology lab, we just simply need to develop the system/flow that works best for the department and for the techs doing the slide preparation. When I look at flow and procedure; accurate, timely patient care is my only concern. Happy Friday!! Heather Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- This message [including any attachments] contains information intended for a specific individual[s] and purpose that may be confidential or otherwise legally protected from disclosure. Any review, use, distribution, disclosure of contents, or copying of the message is strictly prohibited. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately without copying or disclosing the information. From lblazek <@t> digestivespecialists.com Fri Mar 16 15:02:37 2007 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Mar 16 14:58:44 2007 Subject: [Histonet] Lean for Dummies References: Message-ID: <6CBA6DC98A079D408C87250591D9DFB802684D9F@bruexchange.digestivespecialists.com> ME! And I'm outta here. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Orr, Rebecca Sent: Friday, March 16, 2007 3:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Lean for Dummies Hey kids, I was shopping in the local bookstore and came across one of those "...for Dummies" books. This one was for LEAN and SIX Sigma. I opened it and started reading...then my eyes blurred and my head started spinning and I felt a burning sensation... I thought it was because I was reading about this new paradigm we are implementing, but I realized I had spilled my starbucks all over my lap. Happy Friday Who's ready for Green beer and donuts! Becky Orr CLA,HT(ASCP)QIHC Assistant Manager, Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 As it hapened with Janice I can't keep quiet either. Lets talk about LEAN. To start with it is not applicable to the entire histology workflow. The specimen arrives and somebody takes "hold" of it, number, access, describe AND??? that person will have to process it (unless it is a Frozen section). There has to be a delay and don't tell about the Xpress Sakura, it will have to wait until 30 or 40 cassettes will be ready before starting (and what do you call that if you don't want to call it a "batch"?) It will come out after 120 minutes (or more time if it is a regular processor) and again another person will take the cassette in a "nurturing and loving embrace" and will prepare the slide, embed the tissue, section it, WAIT until it dries and will stain it. How long will it take to that "dedicated" histotech to have the slide ready for the pathologist and, most important, how much did that slide cost? What happened to the other cases? Is it a "one specimen a day"lab? Trying to see the histology lab as the production line of a Toyota is unrealistic. Some aspects can be streamlined, others not. The only process that is LEAN (and has always been without knowing it by the way) is the one performed by the histotech rushing over the piece of tissue received from the OR to do a FS, and even there I have seen some mislabellings (like some labs that do not access the tissue to do the FS). Embracing new approaches require a deep study and knowing what we are getting into. Just my opinion (as always)! Ren? J. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Fri Mar 16 16:01:39 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Fri Mar 16 14:59:25 2007 Subject: [Histonet] Lean for Dummies In-Reply-To: Message-ID: I'll tell you one thing that is not LEAN...... my waistline! Beer and donuts for all! Not light beer either. Happy Friday, I am out! Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Orr, Rebecca Sent: Friday, March 16, 2007 2:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Lean for Dummies Hey kids, I was shopping in the local bookstore and came across one of those "...for Dummies" books. This one was for LEAN and SIX Sigma. I opened it and started reading...then my eyes blurred and my head started spinning and I felt a burning sensation... I thought it was because I was reading about this new paradigm we are implementing, but I realized I had spilled my starbucks all over my lap. Happy Friday Who's ready for Green beer and donuts! Becky Orr CLA,HT(ASCP)QIHC Assistant Manager, Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 As it hapened with Janice I can't keep quiet either. Lets talk about LEAN. To start with it is not applicable to the entire histology workflow. The specimen arrives and somebody takes "hold" of it, number, access, describe AND??? that person will have to process it (unless it is a Frozen section). There has to be a delay and don't tell about the Xpress Sakura, it will have to wait until 30 or 40 cassettes will be ready before starting (and what do you call that if you don't want to call it a "batch"?) It will come out after 120 minutes (or more time if it is a regular processor) and again another person will take the cassette in a "nurturing and loving embrace" and will prepare the slide, embed the tissue, section it, WAIT until it dries and will stain it. How long will it take to that "dedicated" histotech to have the slide ready for the pathologist and, most important, how much did that slide cost? What happened to the other cases? Is it a "one specimen a day"lab? Trying to see the histology lab as the production line of a Toyota is unrealistic. Some aspects can be streamlined, others not. The only process that is LEAN (and has always been without knowing it by the way) is the one performed by the histotech rushing over the piece of tissue received from the OR to do a FS, and even there I have seen some mislabellings (like some labs that do not access the tissue to do the FS). Embracing new approaches require a deep study and knowing what we are getting into. Just my opinion (as always)! Ren? J. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Fri Mar 16 15:46:10 2007 From: tkngflght <@t> yahoo.com (Cheryl Kerry) Date: Fri Mar 16 15:46:20 2007 Subject: [Histonet] Temp and permanent job list for Full Staff Message-ID: <000501c7680c$2191fba0$6501a8c0@FSDESKTOP> Hi All-This may post twice and I apologize!! Again we're seeking good travel techs-or good techs who would like to travel~! TEMPS: We have only two temp openings right now, but will have about 12 more within the few weeks. Please don't hesitate to call to ask questions. I'm both a tech and an experienced traveler and I'll tell you the whole story, not just the pretty bits. We put everything in writing BEFORE you leave your house-you should not have to worry about anything other than doing your best work at any of our assignments. --South Central-only 4 weeks and early day shift-a great place to be in the Spring. --North East-Great Lakes Region-13 weeks and night shift (shift differential applies) --I'll post the next round before the end of this month- feel free to call with inquiries. PERMANENT POSTIONS: We also have a HUGE list of open permanent positions, both bench and supervisory, all over the country. Many of these have staffed our temp techs so I know the environments from direct experience. A short list includes (with more added weekly): California Arizona Kansas Florida (we help with licensure) North Carolina South Carolina Ohio Michigan New York (we help with licensure) Texas New Jersey Connecticut Massachusetts Indiana Washington DC Colorado And we of course we will help find labs in specific regions if you want help finding your 'perfect fit' job-let us know! Thank you!! Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. 281.852.9457 office 800.756.3309 fax and alternate phone 281.883.7704 cell Sign up for the FREE newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. From jmahoney <@t> alegent.org Fri Mar 16 16:28:48 2007 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Fri Mar 16 16:29:17 2007 Subject: [Histonet] re-lean Message-ID: <45FAC5C00200003C0000863A@gwia.alegent.org> Those of us who know LEAN know that the FS process described below is not actually LEAN because we would have reduced the walk time and the need to rush the specimen over from the OR. As it hapened with Janice I can't keep quiet either. Lets talk about LEAN. To start with it is not applicable to the entire histology workflow. The specimen arrives and somebody takes "hold" of it, number, access, describe AND??? that person will have to process it (unless it is a Frozen section). There has to be a delay and don't tell about the Xpress Sakura, it will have to wait until 30 or 40 cassettes will be ready before starting (and what do you call that if you don't want to call it a "batch"?) It will come out after 120 minutes (or more time if it is a regular processor) and again another person will take the cassette in a "nurturing and loving embrace" and will prepare the slide, embed the tissue, section it, WAIT until it dries and will stain it. How long will it take to that "dedicated" histotech to have the slide ready for the pathologist and, most important, how much did that slide cost? What happened to the other cases? Is it a "one specimen a day"lab? Trying to see the histology lab as the production line of a Toyota is unrealistic. Some aspects can be streamlined, others not. The only process that is LEAN (and has always been without knowing it by the way) is the one performed by the histotech rushing over the piece of tissue received from the OR to do a FS, and even there I have seen some mislabellings (like some labs that do not access the tissue to do the FS). Embracing new approaches require a deep study and knowing what we are getting into. Just my opinion (as always)! Ren? J. From pvalente <@t> sbcglobal.net Sat Mar 17 23:06:20 2007 From: pvalente <@t> sbcglobal.net (Patricia Valente) Date: Sat Mar 17 23:06:24 2007 Subject: [Histonet] processing times for needle biopsies -using sponges Message-ID: <949938.35363.qm@web81701.mail.mud.yahoo.com> I would like to hear about VIP processing times other folks use for prostate biopsies using biopsy sponges. We currently run an average of 60 cassettes( some days can be 120) all with 2 sponges.- (would prefer to use mesh window cassettes but pathologists prefer the flat and complete sections we (usually) get more easily with the sponges) We rotate 1 of each reagent type ( 95%,100%, Xylene,wax) on processor after each run - this does seem to help with carryover problem. Run is 3hrs 45 min. long Every once in a while we get few nasty hard fragmented cores and other times under dehydration.- These cannot all be blamed on collection or grossing technique ( though we have tried). Seems like processing is uneven.(PV is on) Does anyone use longer times with so many sponges? Has any one tried something to space out cassettes( maybe an empty cassette in-between full ones) to get better contact with solutions? Any other ideas?? Thanks Pat Valente San Antonio TX From pvalente <@t> sbcglobal.net Sat Mar 17 23:06:20 2007 From: pvalente <@t> sbcglobal.net (Patricia Valente) Date: Sat Mar 17 23:06:26 2007 Subject: [Histonet] processing times for needle biopsies -using sponges Message-ID: <949938.35363.qm@web81701.mail.mud.yahoo.com> I would like to hear about VIP processing times other folks use for prostate biopsies using biopsy sponges. We currently run an average of 60 cassettes( some days can be 120) all with 2 sponges.- (would prefer to use mesh window cassettes but pathologists prefer the flat and complete sections we (usually) get more easily with the sponges) We rotate 1 of each reagent type ( 95%,100%, Xylene,wax) on processor after each run - this does seem to help with carryover problem. Run is 3hrs 45 min. long Every once in a while we get few nasty hard fragmented cores and other times under dehydration.- These cannot all be blamed on collection or grossing technique ( though we have tried). Seems like processing is uneven.(PV is on) Does anyone use longer times with so many sponges? Has any one tried something to space out cassettes( maybe an empty cassette in-between full ones) to get better contact with solutions? Any other ideas?? Thanks Pat Valente San Antonio TX From koellingr <@t> comcast.net Sun Mar 18 00:07:57 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Sun Mar 18 00:08:02 2007 Subject: [Histonet] Off topic_recycling xylene Message-ID: <031820070507.3911.45FCC92D0002C09600000F4722007637049D09020704040A0105@comcast.net> This post is an inquiry regarding recycling of xylene in histology labs but is kind of off topic so please simply delete at will. I like to use the HistoNet for scientific inquiry and not debate. I fully support the concept of recycling of xylene in histology labs. But for several years, I've read on the HistoNet many statements from users of various recycling instruments and am curious about their declarative statements. I've read that with (name the brand) recycler, the xylene becomes "purer". The xylene is "better". All the impurities are completely removed. You get purer xylene than you started with. The xylene is absolutely pure. All contaminants, even those that came in the bottle, are removed in recycling. A whole set of declarations that I'm finding hard to accept at face value. When we went through a large study, trying to figure out in a former lab if recycled xylene was good for us, we collected and had analyzed sample after sample from bottles, before recycling after recycling and after multiple recyclings. Had them analyzed by gas chromatography at a good reference lab. Although the recycling worked, we saw little in the change of the readout from pure to recycled. A bit of a change but not much to affect anything. So we recycled and were happy. It is my understanding that tens of millions of pounds of xylenes are produced every year. Histologic grade mixed xylenes (o-, m- and p-) come with a good percentage of ethyl benzene and a minor percentage of various other hydrocarbon contaminants. To remove ethylbenzene and these other contaminants to obtain pure xylenes (needed for upstream processes) requires 100 million dollar chemical plants, hydrogen atmospheres, catalysts and 200 foot high fractionation columns. Do the people who are saying that their lab recyclers are removing these contaminants, follow this xylene stream with critical GC testing? If the xylenes are "better" or "purer" why are there all the warnings to not use recycled xylene for certain procedures? It is "too" pure? Is there such a thing? If this is so easy to accomplish it seems that one could buy a (your brand) recycler, find the cheapest mixed histologic grade xylene you can find, don't use it for histology but simply recycle it, bottle it in 25 or 100 ml bottles as ultrapure for HPLC or gold standard mass spec analysis for 100 times the cost of the original impure xylene and retire rich. I agree xylene recyling is cost-efficient, morally acceptable, environmentally friendly, useful, efficient and everything else. I'm having a hard time accepting that these common lab recyclers do so easily what it takes massive chemical plants and incredible organic chemistry know how to do. If anyone has read this far, and you do recycling of xylene, I'd like to hear about your thoughts on the subject. Raymond Koelling Phenopath Laboratories Seattle, WA From llewllew <@t> shaw.ca Sun Mar 18 00:59:13 2007 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Sun Mar 18 01:01:03 2007 Subject: [Histonet] Off topic_recycling xylene References: <031820070507.3911.45FCC92D0002C09600000F4722007637049D09020704040A0105@comcast.net> Message-ID: <000c01c76922$9ff64570$6400a8c0@yourlk4rlmsu> I think you are forgetting the context in which the comments are made. The impetus for recycling of histological grade xylene as done by histotechs is to avoid disposal costs usually, and as a bonus it reduces the amount spent on new supply. Due to those two factors it becomes cost effective. Again to a histotech, that means the costs are lowered and some money is left over. >From the histotech's viewpoint xylene is good when it is pure enough to remove ethanol or some other dehydrant and make the tissue transparent/translucent and easily infiltrated with paraffin wax. When it is said that recycled xylene is "better", "purer" or some other superlative, they are really saying that it accomplishes that function well, perhaps even slightly better than fresh xylene. My own experience from many years of recycling, is that the recycled stuff was as good as the fresh stuff, but not particularly better. We did use fresh xylene prior to coverslipping, but that was just to be sure and had no factual basis. Bryan Llewellyn ----- Original Message ----- From: To: Sent: Saturday, March 17, 2007 10:07 PM Subject: [Histonet] Off topic_recycling xylene > This post is an inquiry regarding recycling of xylene in histology > labs but is kind of off topic so please simply delete at will. I like to > use the HistoNet for scientific inquiry and not debate. > I fully support the concept of recycling of xylene in histology labs. > But for several years, I've read on the HistoNet many statements from > users of various recycling instruments and am curious about their > declarative statements. I've read that with (name the brand) recycler, > the xylene becomes "purer". The xylene is "better". All the impurities > are completely removed. You get purer xylene than you started with. The > xylene is absolutely pure. All contaminants, even those that came in the > bottle, are removed in recycling. A whole set of declarations that I'm > finding hard to accept at face value. > When we went through a large study, trying to figure out in a former > lab if recycled xylene was good for us, we collected and had analyzed > sample after sample from bottles, before recycling after recycling and > after multiple recyclings. Had them analyzed by gas chromatography at a > good reference lab. Although the recycling worked, we saw little in the > change of the readout from pure to recycled. A bit of a change but not > much to affect anything. So we recycled and were happy. > It is my understanding that tens of millions of pounds of xylenes are > produced every year. Histologic grade mixed xylenes (o-, m- and p-) come > with a good percentage of ethyl benzene and a minor percentage of various > other hydrocarbon contaminants. To remove ethylbenzene and these other > contaminants to obtain pure xylenes (needed for upstream processes) > requires 100 million dollar chemical plants, hydrogen atmospheres, > catalysts and 200 foot high fractionation columns. > Do the people who are saying that their lab recyclers are removing > these contaminants, follow this xylene stream with critical GC testing? > If the xylenes are "better" or "purer" why are there all the warnings to > not use recycled xylene for certain procedures? It is "too" pure? Is > there such a thing? If this is so easy to accomplish it seems that one > could buy a (your brand) recycler, find the cheapest mixed histologic > grade xylene you can find, don't use it for histology but simply recycle > it, bottle it in 25 or 100 ml bottles as ultrapure for HPLC or gold > standard mass spec analysis for 100 times the cost of the original impure > xylene and retire rich. > I agree xylene recyling is cost-efficient, morally acceptable, > environmentally friendly, useful, efficient and everything else. I'm > having a hard time accepting that these common lab recyclers do so easily > what it takes massive chemical plants and incredible organic chemistry > know how to do. > If anyone has read this far, and you do recycling of xylene, I'd like > to hear about your thoughts on the subject. > > Raymond Koelling > Phenopath Laboratories > Seattle, WA > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gu.lang <@t> gmx.at Sun Mar 18 06:09:31 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sun Mar 18 06:09:40 2007 Subject: AW: [Histonet] processing times for needle biopsies -using sponges In-Reply-To: <949938.35363.qm@web81701.mail.mud.yahoo.com> Message-ID: <000301c7694d$e7b62260$6412a8c0@dielangs.at> We use sponges with nearly all our biopsies (40-60). We have no short-run in the VIP, so they are processed with the standard procedure (13 hours in sum). Because the cassettes with the sponges like to swim up, we put them always in the lowest row. I have never experienced hard, brittle prostata biopsies. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Patricia Valente Gesendet: Sonntag, 18. M?rz 2007 05:06 An: histonet@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Betreff: [Histonet] processing times for needle biopsies -using sponges I would like to hear about VIP processing times other folks use for prostate biopsies using biopsy sponges. We currently run an average of 60 cassettes( some days can be 120) all with 2 sponges.- (would prefer to use mesh window cassettes but pathologists prefer the flat and complete sections we (usually) get more easily with the sponges) We rotate 1 of each reagent type ( 95%,100%, Xylene,wax) on processor after each run - this does seem to help with carryover problem. Run is 3hrs 45 min. long Every once in a while we get few nasty hard fragmented cores and other times under dehydration.- These cannot all be blamed on collection or grossing technique ( though we have tried). Seems like processing is uneven.(PV is on) Does anyone use longer times with so many sponges? Has any one tried something to space out cassettes( maybe an empty cassette in-between full ones) to get better contact with solutions? Any other ideas?? Thanks Pat Valente San Antonio TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Eric <@t> ategra.com Fri Mar 16 13:20:04 2007 From: Eric <@t> ategra.com (Eric Dye (ext 223)) Date: Sun Mar 18 08:00:21 2007 Subject: [Histonet] Histology opportunities in your area- Can you help? (update3/16/07) Message-ID: Hi - Histonetters - I have both permanent and full time (permanent) J0BS for HistoTechs throughout the US. How are things at ? These permanent and temporary HISTOTECH J0BS are being filled quickly, don't miss out on your dream position - callmetoday ----------------------------------- Temporary HISTOTECH J0BS : ----------------------------------- (Updated Mar 16 2007) 1. South Carolina - Bench - 3 months (starting in August) 2. Florida (East coast) bench- 6 weeks - Need Florida Licence 3. Rhode Island- 3 months - R.I license eligible- mohs- HOT 4. Western PA - 3 months - Bench HistoTech ------------------------------------------------------- New Permanent HISTOTECH J0BS Listed below ------------------------------------------------------- 1. Central Florida -Histotech-Bench-perm (BrandNew, HOT) 2. Alaska - Histotech - Bench - perm (BrandNew- Hot) 3. Maine - Bench - Perm- Hot(Brand new) 1. Michigan (Ann Arbor Area) Histotech- bench- perm 2. Northern Ohio- Histotech - bench - perm 3. Eastern Pennsylvania - Histotech - Bench - perm 4. Eastern Pennsylvania - Histotech - Bench - perm 5.Florida( Tampa Bay area) Histotech- bench- perm 6.Massachusetts (Greater Boston Area) Histotech- Bench - perm 7.Massachusetts ( Greater Boston Area) Histotech Supervisor 8.Northern Ohio - Histotech - Bench- perm 9. Western Pennsylvania - Bench - perm 10. West Virginia - Bench- perm 11.Massachusetts (Greater Boston Area) Supervisor- perm - HOTHOTHOT!!! 12.Massachusetts (South Boston) - Bench -perm 13. Kentucky (Northwestern)- Bench - perm - 2 openings!! 14. Virginia (Close to North Carolina Border!!) bench- perm 14. Wisconsin - bench - perm ------------------------------------ OTHER HISTOTECH J0BS I have available ---------------------------------- 01. Central Florida - Histotech with some MOHS experience - perm - Hot 02. Hot! Eastern, Massachusetts Seeking Histotechs of all experience levels, Greeaaat paaaay & Great Location - Call Today - 15% Raise Guaranteed! 03.NEWJ0B! Southeast Florida- Histotech - perm - (Need Florida License) 04.Ohio (Southern) - perm - Bench Histotech ( 2 openings) 05.Ohio (Northern) perm- Bench 06.Ohio (Central) perm- Bench 07.Central Florida -perm- Histotech (Need Florida License) 08. Florida (Tampa Bay area) need Florida license 09. Florida, West Coast - both temp & perm openings- Bench Histotech -Very-Hot 10. New York ( Syracuse area) - Bench Histotech- perm 11. New York City (Long Island) - Bench- perm 12. Las Vegas - Bench Histotech- perm 13. Wisconsin- Histology Supervisor- BrandNew -Hot Hot Hot 14. Central-Illinois-Bench-Dermpath- BrandNew -Hot 15. Northern California- Bench- Routine Histology- BrandNew 16. Virginia - Histotech- perm- Bench- 20mins from North Carolona Border -------------------------- end list of Histotech Opportunities ------------------------------------- If you are interested in any of the HISTOTECH J0BS listed above - please call me at 800-466-9919 ext 223 or cell - 407-756-5507. Thank you, Eric Dye-Sr Allied Healthcare Recruiter 800-466-9919 ext 223 or Cell - 407-756-5507 P.S.: Feel free to pass along this email and my phone number to anyone who you think might be interested. P.S.S.: The clients are currently interviewing - and the positions will close soon - so if you are interested, please call me today at 1-800-466-9919 ext 229 --------------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing 800-466-9919 ext 223 - voice 407 671 6075 - fax eric@ategra.com To Learn More About Ategra: http://www.ategra.com ---------------------------------------------------------------- If you received this by mistake, or if you wish not to hear from me, please shoot me a mail to let me know and I'll not mail you again. ---------------------------------------------------------------- ---------------------------------------------------------------- Note: this message is intended for: Histonetters at histonet@lists.utsouthwestern.edu ---------------------------------------------------------------- From rjbuesa <@t> yahoo.com Sun Mar 18 09:16:27 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Mar 18 09:16:32 2007 Subject: [Histonet] Off topic_recycling xylene In-Reply-To: <031820070507.3911.45FCC92D0002C09600000F4722007637049D09020704040A0105@comcast.net> Message-ID: <459953.86398.qm@web61216.mail.yahoo.com> Raymond: Although you wrote that you do not want to start a debate, it is very likely that you will because some things are very "close to the heart" of some histotechs and, as such, they are more "felt" that "factual". I absolutely agree with you that a simple recycler will never get to "pure" xylene, specially if all recyclers star with xylene mixed with impurities. Even the smell (although one should not smell it!!!) if different and you could even tell by it which is "new from the manufacturer" and which is recycled. For all practical purposes (antemedium in tissue processing, dewaxing during staining, and even for film coverslipping) recycled xylene is as useful as "new from the manufacturer" xylene, but it not "better" or "purer", those are just enthusiastic testimonies of those recycling that are so happy doing it that would like everybody to do it. On the other hand, those who recommend to use only "pure from the manufacturer" xylene for certain histology procedures say so because they have some interest in selling the "real thing" and don't want to see their business profits reduced considerable by those who recycle used xylene. Between 1 June/1993 and 31 Dec/2001 I recycled a total of 5,142 gals (at an average of 2.2 gals/day) and I recovered 4,053 gals of xylene, 852 gals of liquid waste and 237 gals of semi-solid waste. The value of the recovered xylene was $26,061 and my total savings were $22,099 My original investment on my very simple B/R recycler was repaid several times over, but I never claimed that the xylene I recycled was "better" than the original one, as a matter of fact, it was as efficient to use, but "somewhat different". Ren? J. koellingr@comcast.net wrote: This post is an inquiry regarding recycling of xylene in histology labs but is kind of off topic so please simply delete at will. I like to use the HistoNet for scientific inquiry and not debate. I fully support the concept of recycling of xylene in histology labs. But for several years, I've read on the HistoNet many statements from users of various recycling instruments and am curious about their declarative statements. I've read that with (name the brand) recycler, the xylene becomes "purer". The xylene is "better". All the impurities are completely removed. You get purer xylene than you started with. The xylene is absolutely pure. All contaminants, even those that came in the bottle, are removed in recycling. A whole set of declarations that I'm finding hard to accept at face value. When we went through a large study, trying to figure out in a former lab if recycled xylene was good for us, we collected and had analyzed sample after sample from bottles, before recycling after recycling and after multiple recyclings. Had them analyzed by gas chromatography at a good reference lab. Although the recycling worked, we saw little in the change of the readout from pure to recycled. A bit of a change but not much to affect anything. So we recycled and were happy. It is my understanding that tens of millions of pounds of xylenes are produced every year. Histologic grade mixed xylenes (o-, m- and p-) come with a good percentage of ethyl benzene and a minor percentage of various other hydrocarbon contaminants. To remove ethylbenzene and these other contaminants to obtain pure xylenes (needed for upstream processes) requires 100 million dollar chemical plants, hydrogen atmospheres, catalysts and 200 foot high fractionation columns. Do the people who are saying that their lab recyclers are removing these contaminants, follow this xylene stream with critical GC testing? If the xylenes are "better" or "purer" why are there all the warnings to not use recycled xylene for certain procedures? It is "too" pure? Is there such a thing? If this is so easy to accomplish it seems that one could buy a (your brand) recycler, find the cheapest mixed histologic grade xylene you can find, don't use it for histology but simply recycle it, bottle it in 25 or 100 ml bottles as ultrapure for HPLC or gold standard mass spec analysis for 100 times the cost of the original impure xylene and retire rich. I agree xylene recyling is cost-efficient, morally acceptable, environmentally friendly, useful, efficient and everything else. I'm having a hard time accepting that these common lab recyclers do so easily what it takes massive chemical plants and incredible organic chemistry know how to do. If anyone has read this far, and you do recycling of xylene, I'd like to hear about your thoughts on the subject. Raymond Koelling Phenopath Laboratories Seattle, WA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Food fight? Enjoy some healthy debate in the Yahoo! Answers Food & Drink Q&A. From rjbuesa <@t> yahoo.com Sun Mar 18 09:31:24 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Mar 18 09:31:32 2007 Subject: [Histonet] processing times for needle biopsies -using sponges In-Reply-To: <949938.35363.qm@web81701.mail.mud.yahoo.com> Message-ID: <587201.16725.qm@web61214.mail.yahoo.com> Patricia: I has been always my objective to develop a processing protocol that could be used with all types of tissues and specimens and for all practical urposes I was able to do it. The only tissue I treated differently where those of the CNS that I always run with longer protocols. My general protocol (for all tissue, and biopsies alike) was: 2 formalin X 30 min. each; 60EthOL + 80EthOL + 95EthOL X 30 min each; 100EthOL s stations (40, 30 and 25 min each); 2 xylenes (25 min each) and 4 paraffin wax (30 min each). When I stopped using xylene altogether for tissue processing things became really better for all tissues and my general protocol was: 2 formalin (25 min each); 80EthOL 90EthOL and 3 100EthOL (all for 15 min each), EIM (2:3:1) at 45?C for 1h30min followed by EIM (1:3:2) at 50?C for 1h 15 min; pure mineral oil at 50?C for 1 hour and 4 paraffin wax stations (45; 20; 20 and 50 min each). EIM is a mixture of Ethanol+Isopropanol+Mineral oil in the indicated proportions. Tissues are processed in a much better way than with xylene and are softer to section, specially the small biopsies, breast, lymph nodes and uterus. I would recommend you to try this procedure. Ren? J. Patricia Valente wrote: I would like to hear about VIP processing times other folks use for prostate biopsies using biopsy sponges. We currently run an average of 60 cassettes( some days can be 120) all with 2 sponges.- (would prefer to use mesh window cassettes but pathologists prefer the flat and complete sections we (usually) get more easily with the sponges) We rotate 1 of each reagent type ( 95%,100%, Xylene,wax) on processor after each run - this does seem to help with carryover problem. Run is 3hrs 45 min. long Every once in a while we get few nasty hard fragmented cores and other times under dehydration.- These cannot all be blamed on collection or grossing technique ( though we have tried). Seems like processing is uneven.(PV is on) Does anyone use longer times with so many sponges? Has any one tried something to space out cassettes( maybe an empty cassette in-between full ones) to get better contact with solutions? Any other ideas?? Thanks Pat Valente San Antonio TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- The fish are biting. Get more visitors on your site using Yahoo! Search Marketing. From koellingr <@t> comcast.net Sun Mar 18 10:06:05 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Sun Mar 18 10:06:11 2007 Subject: [Histonet] Off topic_recycling xylene Message-ID: <031820071506.26376.45FD555D00068B130000670822007637049D09020704040A0105@comcast.net> Rene and Bryan, Thanks for your replies. I can accept explanations of responses that tend toward superlatives and enthusiastic testimonials. Again I agree 100% with reasoning of disposal costs, cost-effectiveness, utility, efficiency and recovery of costs. It is just that a brief search back in the HistoNet archives produces multiple pages, from many users since 1999. The writings include "recycled xylene tends to be purer (than the original)", "recycled xylene is purer than new", "xylene becomes more pure over time the more it is recycled", "more pure with more recycling","had problem with processes after using recycled xylene and it turned out that the xylene was coming out too pure","I do know that xylenes become more pure with recycling". And so on. But I see almost nothing questioning this. Recycling xylene in histology labs is absolutely great. It is just that this all sounds much more than histologic utility or enthusiasm and is written as absolute scientific fact. Am just curious u pon what scientific basis the statements are made. Thanks again. Raymond Koelling PhenoPath Laboratories Seattle, WA -------------- Original message -------------- From: Rene J Buesa Raymond: Although you wrote that you do not want to start a debate, it is very likely that you will because some things are very "close to the heart" of some histotechs and, as such, they are more "felt" that "factual". I absolutely agree with you that a simple recycler will never get to "pure" xylene, specially if all recyclers star with xylene mixed with impurities. Even the smell (although one should not smell it!!!) if different and you could even tell by it which is "new from the manufacturer" and which is recycled. For all practical purposes (antemedium in tissue processing, dewaxing during staining, and even for film coverslipping) recycled xylene is as useful as "new from the manufacturer" xylene, but it not "better" or "purer", those are just enthusiastic testimonies of those recycling that are so happy doing it that would like everybody to do it. On the other hand, those who recommend to use only "pure from the manufacturer" xylene for certain histology procedures say so because they have some interest in selling the "real thing" and don't want to see their business profits reduced considerable by those who recycle used xylene. Between 1 June/1993 and 31 Dec/2001 I recycled a total of 5,142 gals (at an average of 2.2 gals/day) and I recovered 4,053 gals of xylene, 852 gals of liquid waste and 237 gals of semi-solid waste. The value of the recovered xylene was $26,061 and my total savings were $22,099 My original investment on my very simple B/R recycler was repaid several times over, but I never claimed that the xylene I recycled was "better" than the original one, as a matter of fact, it was as efficient to use, but "somewhat different". RenEJ. koellingr@comcast.net wrote: This post is an inquiry regarding recycling of xylene in histology labs but is kind of off topic so please simply delete at will. I like to use the HistoNet for scientific inquiry and not debate. I fully support the concept of recycling of xylene in histology labs. But for several years, I've read on the HistoNet many statements from users of various recycling instruments and am curious about their declarative statements. I've read that with (name the brand) recycler, the xylene becomes "purer". The xylene is "better". All the impurities are completely removed. You get purer xylene than you started with. The xylene is absolutely pure. All contaminants, even those that came in the bottle, are removed in recycling. A whole set of declarations that I'm finding hard to accept at face value. When we went through a large study, trying to figure out in a former lab if recycled xylene was good for us, we collected and had analyzed sample after sample from bottles, before recycling after recycling and after multiple recyclings. Had them analyzed by gas chromatography at a good reference lab. Although the recycling worked, we saw little in the change of the readout from pure to recycled. A bit of a change but not much to affect anything. So we recycled and were happy. It is my understanding that tens of millions of pounds of xylenes are produced every year. Histologic grade mixed xylenes (o-, m- and p-) come with a good percentage of ethyl benzene and a minor percentage of various other hydrocarbon contaminants. To remove ethylbenzene and these other contaminants to obtain pure xylenes (needed for upstream processes) requires 100 million dollar chemical plants, hydrogen atmospheres, catalysts and 200 foot high fractionation columns. Do the people who are saying that their lab recyclers are removing these contaminants, follow this xylene stream with critical GC testing? If the xylenes are "better" or "purer" why are there all the warnings to not use recycled xylene for certain procedures? It is "too" pure? Is there such a thing? If this is so easy to accomplish it seems that one could buy a (your brand) recycler, find the cheapest mixed histologic grade xylene you can find, don't use it for histology but simply recycle it, bottle it in 25 or 100 ml bottles as ultrapure for HPLC or gold standard mass spec analysis for 100 times the cost of the original impure xylene and retire rich. I agree xylene recyling is cost-efficient, morally acceptable, environmentally friendly, useful, efficient and everything else. I'm having a hard time accepting that these common lab recyclers do so easily what it takes massive chemical plants and incredible organic chemistry know how to do. If anyone has read this far, and you do recycling of xylene, I'd like to hear about your thoughts on the subject. Raymond Koelling Phenopath Laboratories Seattle, WA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Food fight? Enjoy some healthy debate in the Yahoo! Answers Food & Drink Q&A. From turkekul <@t> gmail.com Sun Mar 18 15:44:30 2007 From: turkekul <@t> gmail.com (mesruh turkekul) Date: Sun Mar 18 15:44:35 2007 Subject: [Histonet] sectioning spleen Message-ID: Dear Histoneters, I have tried manual paraffin embedding and embedding of spleen in the tissue processor. But I have noticed it is not easy to section. The tissue is so dry and crunchy. I really need to blow hard onto the block while sectioning to get nice sections. Do you have any tips in terms of sectioning. Or maybe fixation, processing? Cheers, Mesruh Turkekul From jqb7 <@t> cdc.gov Sun Mar 18 16:54:50 2007 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Sun Mar 18 16:55:00 2007 Subject: [Histonet] sectioning spleen References: Message-ID: Since spleen is so hemorrhagic I have found once the block is trimmed then prolonged soaking on a slushy, ice water bath prior to sectioning is a huge help. If that is not enough, add some ammonium hydroxide to a separate container and fill with crushed ice and add your trimmed block. Cover to help contain the fumes and this works wonders. Jeanine Bartlett CDc Atlanta -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of mesruh turkekul Sent: Sun 3/18/2007 4:44 PM To: histonet@lists.utsouthwestern.edu Cc: Subject: [Histonet] sectioning spleen Dear Histoneters, I have tried manual paraffin embedding and embedding of spleen in the tissue processor. But I have noticed it is not easy to section. The tissue is so dry and crunchy. I really need to blow hard onto the block while sectioning to get nice sections. Do you have any tips in terms of sectioning. Or maybe fixation, processing? Cheers, Mesruh Turkekul _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayatrikrishna <@t> msn.com Sun Mar 18 19:10:42 2007 From: gayatrikrishna <@t> msn.com (Gayatri Krishna) Date: Sun Mar 18 19:11:17 2007 Subject: [Histonet] (no subject) Message-ID: thank you gayatri From stamptrain <@t> yahoo.com Sun Mar 18 20:59:12 2007 From: stamptrain <@t> yahoo.com (Roger Moretz) Date: Sun Mar 18 20:59:21 2007 Subject: [Histonet] sectioning spleen In-Reply-To: Message-ID: <915560.56339.qm@web55809.mail.re3.yahoo.com> Sounds like your tissue is overdehydrated or overprocessed. Try reducing the time in alcohols and maybe even in paraffin. Reducing alcohol times seems to alleviate over 90% of the "too dry and crunchy" problems we see. Roger Moretz --- mesruh turkekul wrote: > Dear Histoneters, > > I have tried manual paraffin embedding and embedding > of spleen in the > tissue processor. > But I have noticed it is not easy to section. The > tissue is so dry and > crunchy. I really need to blow hard onto the block > while sectioning to > get nice sections. Do you have any tips in terms of > sectioning. Or > maybe fixation, processing? > > > Cheers, > Mesruh Turkekul > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ____________________________________________________________________________________ Food fight? Enjoy some healthy debate in the Yahoo! Answers Food & Drink Q&A. http://answers.yahoo.com/dir/?link=list&sid=396545367 From katri <@t> cogeco.ca Sun Mar 18 21:09:52 2007 From: katri <@t> cogeco.ca (Katri Tuomala) Date: Sun Mar 18 21:09:52 2007 Subject: [Histonet] processing times for needle biopsies -using sponges References: <000301c7694d$e7b62260$6412a8c0@dielangs.at> Message-ID: <001c01c769cb$ae5ac3d0$6a9a9618@Katri> Same here... Katri Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "Gudrun Lang" To: Sent: Sunday, March 18, 2007 7:09 AM Subject: AW: [Histonet] processing times for needle biopsies -using sponges We use sponges with nearly all our biopsies (40-60). We have no short-run in the VIP, so they are processed with the standard procedure (13 hours in sum). Because the cassettes with the sponges like to swim up, we put them always in the lowest row. I have never experienced hard, brittle prostata biopsies. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Patricia Valente Gesendet: Sonntag, 18. M?rz 2007 05:06 An: histonet@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Betreff: [Histonet] processing times for needle biopsies -using sponges I would like to hear about VIP processing times other folks use for prostate biopsies using biopsy sponges. We currently run an average of 60 cassettes( some days can be 120) all with 2 sponges.- (would prefer to use mesh window cassettes but pathologists prefer the flat and complete sections we (usually) get more easily with the sponges) We rotate 1 of each reagent type ( 95%,100%, Xylene,wax) on processor after each run - this does seem to help with carryover problem. Run is 3hrs 45 min. long Every once in a while we get few nasty hard fragmented cores and other times under dehydration.- These cannot all be blamed on collection or grossing technique ( though we have tried). Seems like processing is uneven.(PV is on) Does anyone use longer times with so many sponges? Has any one tried something to space out cassettes( maybe an empty cassette in-between full ones) to get better contact with solutions? Any other ideas?? Thanks Pat Valente San Antonio TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kappeler <@t> patho.unibe.ch Mon Mar 19 02:10:41 2007 From: kappeler <@t> patho.unibe.ch (Andi Kappeler) Date: Mon Mar 19 02:10:51 2007 Subject: [Histonet] novolink kit References: Message-ID: <012001c769f5$b416de10$27955c82@patho.unibe.ch> Hi Louise Novolink works fine, if ... Did you use one of these kits that come (at least) with post-primary block and the Novolink Polymer? We did use one that had even more components, and naive as we were, we applied just the Novolink Polymer (in stead of the ABC/HRP) to a couple of slides with a mouse primary and got no staining at all. Next we did a series of slides that we stained with two different mouse and two different rabbit primaries, leaving away or replacing various components of the Novolink kit. The result was a surprise: you don't need any of these cumbersome bottles except for the Novolink Polymer, if your primaries are rabbit. You need what they call the post primary block, if your primaries are from mouse... In my days, we used to call reagents like the "Post Primary Block" a rabbit-anti-mouse Ig secondary antibody ... In other words, one more company that considers us as stupid "don't-need-to-knows" and sells us a product without telling, what's really in it. Other than that, Novolink works fine, is very sensitive, but dont ask me about my sympathies for companies that consider me stupid... Andi Kappeler Institute of Pathology, University of Bern, Switzerland ----- Original Message ----- From: "louise renton" To: Sent: Friday, March 16, 2007 12:17 PM Subject: [Histonet] novolink kit > hi all > > is anyone using the novolink kit sucessfully? I recently tried it out on > antibodies that work fine with VEctor'snABC kit, only to find that there > is > absolutely NO staining. Any ideas? > > Best regrads & have a great weekend > > -- > Louise Renton > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > "There are nights when the wolves are silent and only the moon howls". > George Carlin > No trees were killed in the sending of this message. > However, many electrons were terribly inconvenienced. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jhnspam <@t> aol.com Mon Mar 19 08:11:29 2007 From: jhnspam <@t> aol.com (jhnspam@aol.com) Date: Mon Mar 19 08:11:57 2007 Subject: [Histonet] Fekete's acid alcohol formalin Message-ID: <8C9383F3E0EE19E-1BA4-4B9A@MBLK-M26.sysops.aol.com> Does anyone have a procedure to prepare Fekete's acid alcohol formalin? Pam Johnson Supervisor, ARC Histology 332 N. Lauderdale St. Memphis, TN 38105-2794 901-495-2272, office 901-495-3112, fax ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From pamvlies <@t> sbcglobal.net Mon Mar 19 08:30:04 2007 From: pamvlies <@t> sbcglobal.net (Pam V) Date: Mon Mar 19 08:30:17 2007 Subject: [Histonet] Gunshot residue in tissue Message-ID: <122262.99337.qm@web81107.mail.mud.yahoo.com> Hi - Back on the 16th of March there was a question regarding a procedure for GSR in tissue. Catching up on some reading, I just found this The email address for contact info for this article is riccardo.zoia@unimi.it Here is the abstract Biotech Histochem. ;81 (4):151-6 17129998 Detection of gunshot residues on cadaveric skin using sodium rhodizonate and a counterstain. [My paper] R Zoja , A Lazzaro , A Battistini , G Gentile We report a staining method for cadaveric tissue using sodium rhodizonate as a skin marker for gunshot residues and a counterstain for the surrounding connective tissue. We studied six well preserved subjects who had died of close range gunshot injury. Skin fragments were removed from the bullet entrance hole including both the disrupted area and adjacent macroscopically intact tissue. Because microscopic examination of postmortem material is difficult after histomorphologic alterations already have occurred as a consequence of postmortem tissue changes, it is necessary to use a staining method that, while detecting gunshot residues, can also make skin cell constituents recognizable from both qualitative and quantitative perspectives. Triphenylmethane dyes (acid fuchsin, aniline blue WS, light green SF yellowish, brilliant green and ethyl green) have proven appropriate for the purpose. It is not free anywhere online, too new I imagine, even via Elsevier but this is a link to the site that manages the journal mentioned above: http://www.informaworld.com/smpp/content~content=a762361057~jumptype=rss Pam Vlies HT-ASCP Evanston Northwestern Hospital Evanston, IL pamvlies@sbcglobal.net From Eric.C.Kellar <@t> questdiagnostics.com Mon Mar 19 08:33:26 2007 From: Eric.C.Kellar <@t> questdiagnostics.com (Kellar, Eric C) Date: Mon Mar 19 08:33:57 2007 Subject: [Histonet] Fekete's acid alcohol formalin Message-ID: <6843061CE6B98E4B96590D4F299618F801583C10@qdcws0117.us.qdx.com> According to Lillie, Fekete's fixative is an acetic acid-alcohol-formalin fixative as follows: 37-40% formaldehyde 10 ml Glacial acetic acid 5 ml 70% alcohol 100 ml Eric C. Kellar Quest Diagnostics Histology Laboratory Supervisor South Florida -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jhnspam@aol.com Sent: Monday, March 19, 2007 9:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fekete's acid alcohol formalin Does anyone have a procedure to prepare Fekete's acid alcohol formalin? Pam Johnson Supervisor, ARC Histology 332 N. Lauderdale St. Memphis, TN 38105-2794 901-495-2272, office 901-495-3112, fax ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bhewlett <@t> cogeco.ca Mon Mar 19 08:45:25 2007 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Mon Mar 19 08:45:37 2007 Subject: [Histonet] Fekete's acid alcohol formalin References: <8C9383F3E0EE19E-1BA4-4B9A@MBLK-M26.sysops.aol.com> Message-ID: <001401c76a2c$d988b970$6500a8c0@mainbox> Pam, You will find the formulation in RD Lillie's book,'Histopathological Technic and Practical Histochemistry', 3rd ED., 1965. on page 39. As follows; 37-40% formaldehyde ... 10.0 mL Glacial acetic acid............5.0 mL 100% ETOH...................70.0 mL D. water (approx 15.0 mL) to make up to............100.0mL Regards, Bryan ----- Original Message ----- From: To: Sent: Monday, March 19, 2007 9:11 AM Subject: [Histonet] Fekete's acid alcohol formalin > Does anyone have a procedure to prepare Fekete's acid alcohol formalin? > > Pam Johnson > Supervisor, ARC Histology > 332 N. Lauderdale St. > Memphis, TN 38105-2794 > 901-495-2272, office > 901-495-3112, fax > ________________________________________________________________________ > AOL now offers free email to everyone. Find out more about what's free > from AOL at AOL.com. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gliuygao <@t> hotmail.com Mon Mar 19 08:49:25 2007 From: gliuygao <@t> hotmail.com (yan gao) Date: Mon Mar 19 08:49:36 2007 Subject: [Histonet] A hepatoma cell line grow in mouse liver, How can I distinguish these two cells? Message-ID: Hi, Histonet. We have a human hepatoma cell line injected into mouse liver. The cell line grown in mouse liver for three weeks. The human hepatoma cells mixed into mouse liver. Does anyone have any idea how to distinguish the human hepatoma cell line in the mouse liver? What marker I should use to distinguish two kinds of cells? Thanks. Yan Gao _________________________________________________________________ [1]Get a FREE Web site, company branded e-mail and more from Microsoft Office Live! References 1. http://g.msn.com/8HMAENUS/2740??PS=47575 From SHargrove <@t> urhcs.org Mon Mar 19 08:58:55 2007 From: SHargrove <@t> urhcs.org (SHargrove@urhcs.org) Date: Mon Mar 19 08:59:10 2007 Subject: [Histonet] Re:processing times for needle biopsies -using sponges In-Reply-To: <20070318180405.2D0AE1C8019@zixvpm01.urhcs.org> Message-ID: Regarding the processing of prostate biopsies, We have a "short run" on one of the VIP's that we use for all biopsies. Our alcohols are graded from 70% - 100%. We used to use sponges for ours and the pathologist thought it caused some breaking. Imagine the tissue bouncing off of the pores of the sponge. Now all needle biopsies are wrapped in paper before processing. We are having much better results .We still use sponges for biopsies. Be sure the sponges are saturated with formalin before use, and they will not float. Susie Hargrove Tech Specialist Histology United Regional Wichita Falls, Texas ********************************************************************** The documents inside this electronic transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party and is required to destroy the information after its stated need has been fulfilled, unless otherwise required by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you received this electronic transmission in error, please notify the sender immediately to arrange for return. ********************************************************************** From rjbuesa <@t> yahoo.com Mon Mar 19 09:25:25 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 19 09:25:32 2007 Subject: [Histonet] sectioning spleen In-Reply-To: Message-ID: <918904.49887.qm@web61217.mail.yahoo.com> Reduce the dehydration times and use intermediate solutions: 100EthOL:xylene=1:2 before the pure xylene and xylene:paraffin=1:2 between xylene and paraffin. Try that the time ratios follow the following: xylene/alcohol = 0.4 and (xylene+paraffin)/alcohol = 1.4 Spleen is difficult because the amount of blood it contains (as you well know). Ren? J. mesruh turkekul wrote: Dear Histoneters, I have tried manual paraffin embedding and embedding of spleen in the tissue processor. But I have noticed it is not easy to section. The tissue is so dry and crunchy. I really need to blow hard onto the block while sectioning to get nice sections. Do you have any tips in terms of sectioning. Or maybe fixation, processing? Cheers, Mesruh Turkekul _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Don't pick lemons. See all the new 2007 cars at Yahoo! Autos. From godsgalnow <@t> aol.com Mon Mar 19 09:52:48 2007 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Mon Mar 19 09:53:14 2007 Subject: [Histonet] validating a new test Message-ID: <8C9384D651D5337-F00-7F5E@mblk-d22.sysops.aol.com> Can anyone out there tell me what you do when you validate a new test? I think we are having to go overboard... Roxanne ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From Jackie.O'Connor <@t> abbott.com Mon Mar 19 10:08:14 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Mon Mar 19 10:08:53 2007 Subject: [Histonet] A hepatoma cell line grow in mouse liver, How can I distinguish these two cells? In-Reply-To: Message-ID: Human mitochondria marker will stain only human cells. Chemicon - catalog number MAB1273. Your mouse is immunocompromised, i.e., SCID? No need to block mouse Ig to use this mAB. I use this constantly to distinguish human tumor xenografts in mice. "yan gao" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/19/2007 08:49 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] A hepatoma cell line grow in mouse liver, How can I distinguish these two cells? Hi, Histonet. We have a human hepatoma cell line injected into mouse liver. The cell line grown in mouse liver for three weeks. The human hepatoma cells mixed into mouse liver. Does anyone have any idea how to distinguish the human hepatoma cell line in the mouse liver? What marker I should use to distinguish two kinds of cells? Thanks. Yan Gao _________________________________________________________________ [1]Get a FREE Web site, company branded e-mail and more from Microsoft Office Live! References 1. http://g.msn.com/8HMAENUS/2740??PS=47575 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From indytreegers <@t> sbcglobal.net Mon Mar 19 10:12:14 2007 From: indytreegers <@t> sbcglobal.net (Lyn Treeger) Date: Mon Mar 19 10:12:22 2007 Subject: [Histonet] Florida License? Message-ID: <966028.63622.qm@web81907.mail.mud.yahoo.com> Hi, Can any of you tell me how the Florida License requirements compare to the HT(ASCP)? I am registered with ASCP, but am curious about the FL testing process. My husband just heard about a remotely possible job opportunity in Melbourne, FL, and I am wondering what it would take for me to, well, basically, get a job down there? Thanks in advance for any information you can give me, Lyn Lyn Treeger, B.S. HT(ASCP) From godsgalnow <@t> aol.com Mon Mar 19 10:21:23 2007 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Mon Mar 19 10:21:38 2007 Subject: [Histonet] Florida License? In-Reply-To: <966028.63622.qm@web81907.mail.mud.yahoo.com> References: <966028.63622.qm@web81907.mail.mud.yahoo.com> Message-ID: <8C9385163A75422-F00-8121@mblk-d22.sysops.aol.com> If you have your ASCP, there is not another test to take for Florida, just, CEU classes that you need. Check out the website link below. http://www.doh.state.fl.us/mqa/ Roxanne Soto HT(ASCP)QIHC Laboratory Supervisor Physicians RightPath Tampa, Florida -----Original Message----- From: indytreegers@sbcglobal.net To: histonet@lists.utsouthwestern.edu Sent: Mon, 19 Mar 2007 11:12 AM Subject: [Histonet] Florida License? Hi, Can any of you tell me how the Florida License requirements compare to the HT(ASCP)? I am registered with ASCP, but am curious about the FL testing process. My husband just heard about a remotely possible job opportunity in Melbourne, FL, and I am wondering what it would take for me to, well, basically, get a job down there? Thanks in advance for any information you can give me, Lyn Lyn Treeger, B.S. HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From rjbuesa <@t> yahoo.com Mon Mar 19 10:23:57 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 19 10:24:07 2007 Subject: [Histonet] Florida License? In-Reply-To: <966028.63622.qm@web81907.mail.mud.yahoo.com> Message-ID: <45184.12441.qm@web61221.mail.yahoo.com> Lyn: In Florida the hiring standard almost always reads: "Florida licensed or eligible" and any HT (ASCP) is eligible under Florida standards. You usually are hired with your ASCP and can work until you take (and pass) the test that is of similar difficulty as the ASCP, although "difficulty" is a very relative qualifier because it depends on your knowledge of the subject and varies from individual to individual. Ren? J. Lyn Treeger wrote: Hi, Can any of you tell me how the Florida License requirements compare to the HT(ASCP)? I am registered with ASCP, but am curious about the FL testing process. My husband just heard about a remotely possible job opportunity in Melbourne, FL, and I am wondering what it would take for me to, well, basically, get a job down there? Thanks in advance for any information you can give me, Lyn Lyn Treeger, B.S. HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- We won't tell. Get more on shows you hate to love (and love to hate): Yahoo! TV's Guilty Pleasures list. From jerry.santiago <@t> jax.ufl.edu Mon Mar 19 10:47:55 2007 From: jerry.santiago <@t> jax.ufl.edu (Santiago, Jerry) Date: Mon Mar 19 10:48:43 2007 Subject: [Histonet] Florida License? In-Reply-To: <8C9385163A75422-F00-8121@mblk-d22.sysops.aol.com> Message-ID: <816DC61E1730E843A0BCF4CCFA1EFCF308925B@jaxmail.umc.ufl.edu> Lyn, With your HT(ASCP) you automatically can apply for a technician license. My recommendation is to apply for a technologist license which can be done by using the following routes: 1) HT(ASCP), 5 years of pertinent laboratory experience & 48 contact hours in advanced techniques such as IHC & Molecular 2) HT(ASCP) & QIHC(ASCP) The state test is no longer available, the only acceptance is the ASCP. Jerry Santiago, BS, HTL(ASCP)QIHC Shands Jacksonville Jacksonville, FL 904-244-6149 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of godsgalnow@aol.com Sent: Monday, March 19, 2007 11:21 AM To: indytreegers@sbcglobal.net; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Florida License? If you have your ASCP, there is not another test to take for Florida, just, CEU classes that you need. Check out the website link below. http://www.doh.state.fl.us/mqa/ Roxanne Soto HT(ASCP)QIHC Laboratory Supervisor Physicians RightPath Tampa, Florida -----Original Message----- From: indytreegers@sbcglobal.net To: histonet@lists.utsouthwestern.edu Sent: Mon, 19 Mar 2007 11:12 AM Subject: [Histonet] Florida License? Hi, Can any of you tell me how the Florida License requirements compare to the HT(ASCP)? I am registered with ASCP, but am curious about the FL testing process. My husband just heard about a remotely possible job opportunity in Melbourne, FL, and I am wondering what it would take for me to, well, basically, get a job down there? Thanks in advance for any information you can give me, Lyn Lyn Treeger, B.S. HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Mon Mar 19 10:53:05 2007 From: tkngflght <@t> yahoo.com (Cheryl Kerry) Date: Mon Mar 19 10:53:06 2007 Subject: [Histonet] Florida-- In-Reply-To: <8C9385163A75422-F00-8121@mblk-d22.sysops.aol.com> References: <966028.63622.qm@web81907.mail.mud.yahoo.com> <8C9385163A75422-F00-8121@mblk-d22.sysops.aol.com> Message-ID: <006101c76a3e$af7908d0$6501a8c0@FSDESKTOP> As I understand it you cannot work in Florida without your license already in place. Used to in some cases, but no longer. ASCP pretty much guarantees you a license but you still have to do all the paperwork and wait. There is no 'temporary' or 'work while waiting' like there is for NY State. The best thing is to download the regs and read them. (See link Roxanne included below). Don't let the length freak you out--it's written in English, not technobabble, and the information you need is in the middle under the requirements section. Be sure to read the part that applies to the license you want (HT/2yr or HTL/4yr levels) Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. 281.852.9457 800.756.3309 Sign up for the FREE newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: Monday, March 19, 2007 10:21 AM To: indytreegers@sbcglobal.net; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Florida License? If you have your ASCP, there is not another test to take for Florida, just, CEU classes that you need. Check out the website link below. http://www.doh.state.fl.us/mqa/ Roxanne Soto HT(ASCP)QIHC Laboratory Supervisor Physicians RightPath Tampa, Florida -----Original Message----- From: indytreegers@sbcglobal.net To: histonet@lists.utsouthwestern.edu Sent: Mon, 19 Mar 2007 11:12 AM Subject: [Histonet] Florida License? Hi, Can any of you tell me how the Florida License requirements compare to the HT(ASCP)? I am registered with ASCP, but am curious about the FL testing process. My husband just heard about a remotely possible job opportunity in Melbourne, FL, and I am wondering what it would take for me to, well, basically, get a job down there? Thanks in advance for any information you can give me, Lyn Lyn Treeger, B.S. HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sonya.martin <@t> soton.ac.uk Mon Mar 19 11:53:03 2007 From: sonya.martin <@t> soton.ac.uk (Martin S.) Date: Mon Mar 19 11:53:35 2007 Subject: [Histonet] Glucose oxidase block for endog peroxidase Message-ID: <71437982F5B13A4D9A5B2669BDB89EE4023E353F@ISS-CL-EX-V1.soton.ac.uk> Hi All, I want to try the glucose oxidase block for endogenous peroxidase. I have the following recipe (kindly provided by Gayle Callis) but I cant find any suppliers of beta glucose. Sigma no longer stock it and all other companies I usually use dont have it. Where do you guys get it from? I'm in the UK but can get it from the US if needs be! Glucose Beta-D(+) Glucose, 180.12 (ICN) 0.180 g Glucose oxidase (Sigma G 6641) 0.005 g Sodium azide 0.0065g DPBS 50 ml Thanks Sonya From innvx <@t> sbcglobal.net Mon Mar 19 12:06:44 2007 From: innvx <@t> sbcglobal.net (INNOVEX BIOSCIENCES) Date: Mon Mar 19 12:06:52 2007 Subject: [Histonet] reply to Claire Ingles- Please post Message-ID: <43067.93132.qm@web82013.mail.mud.yahoo.com> Dear Claire, Per our conversation this morning, please note that the timing for HMB45 Primary Antibody and Innovex Two-step Alk Phos was not enough instead of 10-10. Best results are obtained at 30 for antibody and 30 for second step Alk Phos. This information is in the data sheet. Zahra Naser, PH.D. Innovex Biosciences From Heather.Nymeyer <@t> interiorhealth.ca Mon Mar 19 12:09:17 2007 From: Heather.Nymeyer <@t> interiorhealth.ca (Nymeyer, Heather) Date: Mon Mar 19 12:09:45 2007 Subject: [Histonet] Metabolic bone disease Message-ID: I need to contact a laboratory that has a protocol established for the testing of Metabolic Bone Disease in humans. If you could please forward me your laboratory's name, contact person and the laboratory's location it would be appreciated. Heather D. Nymeyer, RT, CEBT Charge Technologist, Anatomic Pathology Royal Inland Hospital, Kamloops, BC. 250-314-2664 heather.nymeyer@interiorhealth.ca From gcallis <@t> montana.edu Mon Mar 19 12:11:05 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Mar 19 12:11:14 2007 Subject: [Histonet] Glucose oxidase block for endog peroxidase In-Reply-To: <71437982F5B13A4D9A5B2669BDB89EE4023E353F@ISS-CL-EX-V1.soto n.ac.uk> References: <71437982F5B13A4D9A5B2669BDB89EE4023E353F@ISS-CL-EX-V1.soton.ac.uk> Message-ID: <6.0.0.22.1.20070319110619.01b38628@gemini.msu.montana.edu> Sonya, In the recipe, I indicated the company after the glucose you need to buy. It is a chemical company called ICN, and they have a website. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 At 10:53 AM 3/19/2007, you wrote: >Hi All, > >I want to try the glucose oxidase block for endogenous peroxidase. I >have the following recipe (kindly provided by Gayle Callis) but I cant >find any suppliers of beta glucose. Sigma no longer stock it and all >other companies I usually use dont have it. Where do you guys get it >from? I'm in the UK but can get it from the US if needs be! > >Glucose Beta-D(+) Glucose, 180.12 (ICN) >0.180 g From Vickroy.Jim <@t> mhsil.com Mon Mar 19 12:17:33 2007 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Mon Mar 19 12:19:00 2007 Subject: [Histonet] temperature range of tissue floatation baths Message-ID: Recently we were cited for not measuring the temperature of our water baths (tissue floatation baths). We use Richard Allan Type 9 paraffin. I am trying to put down a range of acceptable temperatures for our daily quality control. Any ideas? Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From FUNKM <@t> mercyhealth.com Mon Mar 19 12:23:21 2007 From: FUNKM <@t> mercyhealth.com (Marcia Funk) Date: Mon Mar 19 12:23:52 2007 Subject: [Histonet] Lean what not use 5 S Message-ID: <45FE80B9020000AC00000842@nodcmsngwia1.trinity-health.org> Hello,. One more thing on Lean. Why are labs spending the BIG money to have a company come in and take them through the Lean concept. Most lab I know I hope have been following the 5 " S" that they leaned in leadership. If not maybe they need to look at there leadership. SORT - Remove all items from the workplace that not needed SET IN ORDER - Arrange items so that they are easy to use and label them so that they are easy to find. SHINE - Cleanliness SCANDALIZE - Creating a consistent way that tasks and procedures are carried out. SUSTAIN - Properly maintaining procedures Sounds pretty simple to me. That how I have run our lab and everyone loves to come to work, and so do I. Have a great Monday. Marcia From Melissa.Gonzalez <@t> cellgenesys.com Mon Mar 19 12:25:54 2007 From: Melissa.Gonzalez <@t> cellgenesys.com (Melissa Gonzalez) Date: Mon Mar 19 12:26:07 2007 Subject: [Histonet] RE:QDots In-Reply-To: <20070315171801.F1D9A27F071@mail.cellgenesys.com> References: <20070315171801.F1D9A27F071@mail.cellgenesys.com> Message-ID: Alejandro, Look at the instructions carefully for mounting & coverslipping your slides when using Qdots. They are very sensitive to quenching if you don't use the proper mounting media. I kind of gave up on them, and would recommend you use Alexa Fluors instead. Or talk to Invitrogen's tech support regarding your specific applications, they have very good tech support... GL, Melissa ------------------------------ Message: 27 Date: Thu, 15 Mar 2007 17:28:31 +0100 From: Alejandro Ortiz Stern Subject: [Histonet] Qdots To: histonet@lists.utsouthwestern.edu Message-ID: <45F9742F.6050905@ipmc.cnrs.fr> Content-Type: text/plain; charset=ISO-8859-1; format=flowed I would like to know if anyone has experience with Streptavidine-Qdots for the labeling of Acetone post-fixed slides (IHF). How do you wash the slides in order to get the best signal to noise ratio? Thank you for your input on the matter. -- Alejandro Ortiz Stern M.D. Ph.D., Postdoctoral Fellow Institut National de la Sant? et de la Recherche M?dicale, E0344 Universit? de Nice-Sophia-Antipolis Institut de Pharmacologie Mol?culaire et Cellulaire 660 Route des Lucioles - 06560 Valbonne - FRANCE TEL: 33 (4) 93 95 77 80 - FAX: 33 (4) 93 95 77 08 From EWURDAK <@t> CSBSJU.EDU Mon Mar 19 12:35:52 2007 From: EWURDAK <@t> CSBSJU.EDU (Wurdak, Elizabeth) Date: Mon Mar 19 12:36:00 2007 Subject: [Histonet] Gill's hematoxylin In-Reply-To: Message-ID: Hello Histonetters, We have decided to switch from Harris' hematoxylin to Gill's to avoid using mercury. I have a couple of recipes for Gill's hematoxylin. They both call for aluminum sulfate and sodium iodate. I have only ammonia alum, NH4Al(SO4)2x12H2O, or potassium alum, KAl(SO4)2x12H2O and sodium periodate on hand. Can I use these reagents instead and do I need to modify the quantities of each. The IHC World recipe requires 0.2g sodium iodate and 20g aluminum sulfate for 1020 ml of the single strength hematoxylin (2g) solution. Thanks for any help you can give me. Liz Elizabeth Wurdak, PhD Biology Department Saint John?s University Collegeville, MN 56321 Tel: (320) 363-3177 On 3/19/07 8:58 AM, "SHargrove@urhcs.org" wrote: > Regarding the processing of prostate biopsies, We have a "short run" on one > of the VIP's that we use for all biopsies. Our alcohols are graded from 70% > - 100%. We used to use sponges for ours and the pathologist thought it > caused some breaking. Imagine the tissue bouncing off of the pores of the > sponge. Now all needle biopsies are wrapped in paper before processing. We > are having much better results .We still use sponges for biopsies. Be sure > the sponges are saturated with formalin before use, and they will not > float. > Susie Hargrove > Tech Specialist Histology > United Regional > Wichita Falls, Texas > > ********************************************************************** > The documents inside this electronic transmission contain > confidential information belonging to the sender that is legally > privileged. This information is intended only for the use of the > individual or entity named above. The authorized recipient of this > information is prohibited from disclosing this information to any > other party and is required to destroy the information after its > stated need has been fulfilled, unless otherwise required by law. > > If you are not the intended recipient, you are hereby notified that > any disclosure, copying, distribution, or action taken in reliance > on the contents of these documents is strictly prohibited. If you > received this electronic transmission in error, please notify the > sender immediately to arrange for return. > ********************************************************************** > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amartinez <@t> carisdx.com Mon Mar 19 12:40:19 2007 From: amartinez <@t> carisdx.com (Martinez, Angela) Date: Mon Mar 19 12:40:54 2007 Subject: [Histonet] Opportunity Message-ID: <9B8A3AC772C7F64680392A7CB8FBFB0F01CFD9B1@s-irv-ex301.PathologyPartners.intranet> Caris would like to announce a new job opening in the Phoenix Arizona location. The position is for a full time Histotechnologist. This position will entail routine Histology including: specimen processing, embedding, microtomy, staining, and immunohistochemistry. Candidates should be HT certified and should possess a BS degree. Further, candidates should have 1-3 years experience in embedding and sectioning of surgical specimens in a certified Histopathology laboratory. Excellent communication skills and the ability to work in a high volume environment is required. To apply, please e-mail resume to: amartine@carisdx.com Angela Martinez Human Resources Coordinator Caris Diagnostics 8400 Esters Blvd. Suite 190 Irving, TX 75063 214-277-8700 Phone 214-596-7490- Fax 214-538-6235 Cell From pkarlisch <@t> psu.edu Mon Mar 19 12:41:37 2007 From: pkarlisch <@t> psu.edu (Patricia Karlisch) Date: Mon Mar 19 12:42:18 2007 Subject: [Histonet] Unsubcribe Message-ID: <45FE9311.D9B9.008C.0@psu.edu> Please unsubscribe due to vacation. Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. From rjbuesa <@t> yahoo.com Mon Mar 19 13:09:57 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 19 13:10:06 2007 Subject: [Histonet] temperature range of tissue floatation baths In-Reply-To: Message-ID: <387690.13309.qm@web61215.mail.yahoo.com> Jim: The water bath temperature has to be bellow the melting point (MP) of the paraffin. I always had ours at 45?C and that was what we had in our logs. Not always it was 45?C, sometimes it deviated 1 or 2?C and that was acceptable but you should regulate yours according with the MP of the paraffin you use. Ren? J. "Vickroy, Jim" wrote: Recently we were cited for not measuring the temperature of our water baths (tissue floatation baths). We use Richard Allan Type 9 paraffin. I am trying to put down a range of acceptable temperatures for our daily quality control. Any ideas? Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Bored stiff? Loosen up... Download and play hundreds of games for free on Yahoo! Games. From rjbuesa <@t> yahoo.com Mon Mar 19 13:12:05 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 19 13:12:10 2007 Subject: [Histonet] Lean what not use 5 S In-Reply-To: <45FE80B9020000AC00000842@nodcmsngwia1.trinity-health.org> Message-ID: <360089.29497.qm@web61217.mail.yahoo.com> The "big money" as you say is spent because Lean is a trendy fad. Ren? J. Marcia Funk wrote: Hello,. One more thing on Lean. Why are labs spending the BIG money to have a company come in and take them through the Lean concept. Most lab I know I hope have been following the 5 " S" that they leaned in leadership. If not maybe they need to look at there leadership. SORT - Remove all items from the workplace that not needed SET IN ORDER - Arrange items so that they are easy to use and label them so that they are easy to find. SHINE - Cleanliness SCANDALIZE - Creating a consistent way that tasks and procedures are carried out. SUSTAIN - Properly maintaining procedures Sounds pretty simple to me. That how I have run our lab and everyone loves to come to work, and so do I. Have a great Monday. Marcia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- No need to miss a message. Get email on-the-go with Yahoo! Mail for Mobile. Get started. From Melissa.Gonzalez <@t> cellgenesys.com Mon Mar 19 13:13:18 2007 From: Melissa.Gonzalez <@t> cellgenesys.com (Melissa Gonzalez) Date: Mon Mar 19 13:13:28 2007 Subject: [Histonet] RE: Human vs mouse cell In-Reply-To: <20070319175108.11A3F27F00C@mail.cellgenesys.com> References: <20070319175108.11A3F27F00C@mail.cellgenesys.com> Message-ID: Jackie, does this work well on FFPE sections as well? I just looked up the product and it looks like it should, w/ citrate buffer & perhaps with amplification. Just wondering if it is finicky or actually pretty good signal... Thanks Melissa ------------------------------ Message: 15 Date: Mon, 19 Mar 2007 10:08:14 -0500 From: "Jackie M O'Connor" Subject: Re: [Histonet] A hepatoma cell line grow in mouse liver, How can I distinguish these two cells? To: "yan gao" Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Human mitochondria marker will stain only human cells. Chemicon - catalog number MAB1273. Your mouse is immunocompromised, i.e., SCID? No need to block mouse Ig to use this mAB. I use this constantly to distinguish human tumor xenografts in mice. From rjbuesa <@t> yahoo.com Mon Mar 19 13:14:48 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 19 13:14:55 2007 Subject: [Histonet] Gill's hematoxylin In-Reply-To: Message-ID: <82282.31772.qm@web61214.mail.yahoo.com> If you use what you have you will end with another hematoxylin. Try it, maybe it is good afterall! Ren? J. "Wurdak, Elizabeth" wrote: Hello Histonetters, We have decided to switch from Harris' hematoxylin to Gill's to avoid using mercury. I have a couple of recipes for Gill's hematoxylin. They both call for aluminum sulfate and sodium iodate. I have only ammonia alum, NH4Al(SO4)2x12H2O, or potassium alum, KAl(SO4)2x12H2O and sodium periodate on hand. Can I use these reagents instead and do I need to modify the quantities of each. The IHC World recipe requires 0.2g sodium iodate and 20g aluminum sulfate for 1020 ml of the single strength hematoxylin (2g) solution. Thanks for any help you can give me. Liz Elizabeth Wurdak, PhD Biology Department Saint John?s University Collegeville, MN 56321 Tel: (320) 363-3177 On 3/19/07 8:58 AM, "SHargrove@urhcs.org" wrote: > Regarding the processing of prostate biopsies, We have a "short run" on one > of the VIP's that we use for all biopsies. Our alcohols are graded from 70% > - 100%. We used to use sponges for ours and the pathologist thought it > caused some breaking. Imagine the tissue bouncing off of the pores of the > sponge. Now all needle biopsies are wrapped in paper before processing. We > are having much better results .We still use sponges for biopsies. Be sure > the sponges are saturated with formalin before use, and they will not > float. > Susie Hargrove > Tech Specialist Histology > United Regional > Wichita Falls, Texas > > ********************************************************************** > The documents inside this electronic transmission contain > confidential information belonging to the sender that is legally > privileged. This information is intended only for the use of the > individual or entity named above. The authorized recipient of this > information is prohibited from disclosing this information to any > other party and is required to destroy the information after its > stated need has been fulfilled, unless otherwise required by law. > > If you are not the intended recipient, you are hereby notified that > any disclosure, copying, distribution, or action taken in reliance > on the contents of these documents is strictly prohibited. If you > received this electronic transmission in error, please notify the > sender immediately to arrange for return. > ********************************************************************** > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss an email again! Yahoo! Toolbar alerts you the instant new Mail arrives. Check it out. From mthomas <@t> littonlab.com Mon Mar 19 13:15:17 2007 From: mthomas <@t> littonlab.com (Marla Thomas) Date: Mon Mar 19 13:15:31 2007 Subject: [Histonet] temperature range of floatation baths Message-ID: <005001c76a52$8c8c0e80$9d35a8c0@LittonPath.local> Well, back in the day we figured the "plastic point" of the paraffin. Which is 10-15 degrees lower than the melting point. If you melting point is 56 your range should be 41-46 degrees. Usually the mid temp worked the best 43-44. Marla Thomas, HT(ASCP) HIPAA/Compliance/IT Manager Litton Pathology Associates, PC 700 NW Hunter Drive Blue Springs, MO 64015 816-229-6449, fax 816-874-4400 This e-mail, including attachments, may include confidential and/or proprietary information, and may be used only by the person or entity to which it is addressed. If the reader of this e-mail is not the intended recipient or his/her authorized agent, the reader is hereby notified that any dissemination, distribution or copying of this e-mail is prohibited. If you have received this e-mail in error, please notify the sender by replying to this message and delete this e-mail immediately. From settembr <@t> umdnj.edu Mon Mar 19 13:24:30 2007 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Mon Mar 19 13:25:39 2007 Subject: [Histonet] temperature range of tissue floatation baths Message-ID: I believe the idea is to have your water bath about 5 to 10 degrees cooler than your paraffin's melting point. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> "Vickroy, Jim" 03/19/07 1:17 PM >>> Recently we were cited for not measuring the temperature of our water baths (tissue floatation baths). We use Richard Allan Type 9 paraffin. I am trying to put down a range of acceptable temperatures for our daily quality control. Any ideas? Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Mon Mar 19 13:26:28 2007 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Mon Mar 19 13:26:26 2007 Subject: [Histonet] temperature range of tissue floatation baths In-Reply-To: Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F46C@lmhsmail.lmhealth.org> We also use type 9 paraffin. Our range is 40c to 48c. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Vickroy, Jim Sent: Monday, March 19, 2007 1:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] temperature range of tissue floatation baths Recently we were cited for not measuring the temperature of our water baths (tissue floatation baths). We use Richard Allan Type 9 paraffin. I am trying to put down a range of acceptable temperatures for our daily quality control. Any ideas? Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From llewllew <@t> shaw.ca Mon Mar 19 13:33:39 2007 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Mon Mar 19 13:34:24 2007 Subject: [Histonet] Gill's hematoxylin References: Message-ID: <003601c76a55$1d1aab80$6500a8c0@yourlk4rlmsu> Got to http://stainsfile.info/StainsFile/stain/hemindex.htm there are dozens of formulae there, including a variation of Harris that uses sodium iodate instead of mercuric oxide. Bryan Llewellyn ----- Original Message ----- From: "Wurdak, Elizabeth" To: "Histonet" Sent: Monday, March 19, 2007 10:35 AM Subject: [Histonet] Gill's hematoxylin Hello Histonetters, We have decided to switch from Harris' hematoxylin to Gill's to avoid using mercury. I have a couple of recipes for Gill's hematoxylin. They both call for aluminum sulfate and sodium iodate. I have only ammonia alum, NH4Al(SO4)2x12H2O, or potassium alum, KAl(SO4)2x12H2O and sodium periodate on hand. Can I use these reagents instead and do I need to modify the quantities of each. The IHC World recipe requires 0.2g sodium iodate and 20g aluminum sulfate for 1020 ml of the single strength hematoxylin (2g) solution. Thanks for any help you can give me. Liz Elizabeth Wurdak, PhD Biology Department Saint John?s University Collegeville, MN 56321 Tel: (320) 363-3177 On 3/19/07 8:58 AM, "SHargrove@urhcs.org" wrote: > Regarding the processing of prostate biopsies, We have a "short run" on > one > of the VIP's that we use for all biopsies. Our alcohols are graded from > 70% > - 100%. We used to use sponges for ours and the pathologist thought it > caused some breaking. Imagine the tissue bouncing off of the pores of the > sponge. Now all needle biopsies are wrapped in paper before processing. We > are having much better results .We still use sponges for biopsies. Be sure > the sponges are saturated with formalin before use, and they will not > float. > Susie Hargrove > Tech Specialist Histology > United Regional > Wichita Falls, Texas > > ********************************************************************** > The documents inside this electronic transmission contain > confidential information belonging to the sender that is legally > privileged. This information is intended only for the use of the > individual or entity named above. The authorized recipient of this > information is prohibited from disclosing this information to any > other party and is required to destroy the information after its > stated need has been fulfilled, unless otherwise required by law. > > If you are not the intended recipient, you are hereby notified that > any disclosure, copying, distribution, or action taken in reliance > on the contents of these documents is strictly prohibited. If you > received this electronic transmission in error, please notify the > sender immediately to arrange for return. > ********************************************************************** > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From duncanwood1 <@t> fsmail.net Mon Mar 19 13:51:08 2007 From: duncanwood1 <@t> fsmail.net (Duncan Wood) Date: Mon Mar 19 13:51:17 2007 Subject: [Histonet] RE: Histonet Digest, Vol 40, Issue 27 Gill's Haemaqtoxylin Message-ID: <32833121.48111174330268088.JavaMail.www@wwinf3101> If you want to use Gill's then you must use the Aluminium Sulphate, If you want to use the higher strengths of Gill's (double and triple strengths) then make sure that ALL the proportions are done properly, other wise it won't work. It is possible to use Harris's that is mercury free if you are happier with that, but the Haematoxylin content needs to be increased by about 50%. can give you fuller details if you need them. Good staining! Duncan Wood, Consultant, Clin-Tech Ltd., UK ======================================== Message Received: Mar 19 2007, 06:01 PM From: histonet-request@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Cc: Subject: Histonet Digest, Vol 40, Issue 27 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Gill's hematoxylin (Wurdak, Elizabeth) 2. Opportunity (Martinez, Angela) 3. Unsubcribe (Patricia Karlisch) ---------------------------------------------------------------------- Message: 1 Date: Mon, 19 Mar 2007 12:35:52 -0500 From: "Wurdak, Elizabeth" Subject: [Histonet] Gill's hematoxylin To: Histonet Message-ID: Content-Type: text/plain; charset="ISO-8859-1" Hello Histonetters, We have decided to switch from Harris' hematoxylin to Gill's to avoid using mercury. I have a couple of recipes for Gill's hematoxylin. They both call for aluminum sulfate and sodium iodate. I have only ammonia alum, NH4Al(SO4)2x12H2O, or potassium alum, KAl(SO4)2x12H2O and sodium periodate on hand. Can I use these reagents instead and do I need to modify the quantities of each. The IHC World recipe requires 0.2g sodium iodate and 20g aluminum sulfate for 1020 ml of the single strength hematoxylin (2g) solution. Thanks for any help you can give me. Liz Elizabeth Wurdak, PhD Biology Department Saint John?s University Collegeville, MN 56321 Tel: (320) 363-3177 On 3/19/07 8:58 AM, "SHargrove@urhcs.org" wrote: > Regarding the processing of prostate biopsies, We have a "short run" on one > of the VIP's that we use for all biopsies. Our alcohols are graded from 70% > - 100%. We used to use sponges for ours and the pathologist thought it > caused some breaking. Imagine the tissue bouncing off of the pores of the > sponge. Now all needle biopsies are wrapped in paper before processing. We > are having much better results .We still use sponges for biopsies. Be sure > the sponges are saturated with formalin before use, and they will not > float. > Susie Hargrove > Tech Specialist Histology > United Regional > Wichita Falls, Texas > > ********************************************************************** > The documents inside this electronic transmission contain > confidential information belonging to the sender that is legally > privileged. This information is intended only for the use of the > individual or entity named above. The authorized recipient of this > information is prohibited from disclosing this information to any > other party and is required to destroy the information after its > stated need has been fulfilled, unless otherwise required by law. > > If you are not the intended recipient, you are hereby notified that > any disclosure, copying, distribution, or action taken in reliance > on the contents of these documents is strictly prohibited. If you > received this electronic transmission in error, please notify the > sender immediately to arrange for return. > ********************************************************************** > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Mon, 19 Mar 2007 12:40:19 -0500 From: "Martinez, Angela" Subject: [Histonet] Opportunity To: Message-ID: <9B8A3AC772C7F64680392A7CB8FBFB0F01CFD9B1@s-irv-ex301.PathologyPartners.intranet> Content-Type: text/plain; charset="us-ascii" Caris would like to announce a new job opening in the Phoenix Arizona location. The position is for a full time Histotechnologist. This position will entail routine Histology including: specimen processing, embedding, microtomy, staining, and immunohistochemistry. Candidates should be HT certified and should possess a BS degree. Further, candidates should have 1-3 years experience in embedding and sectioning of surgical specimens in a certified Histopathology laboratory. Excellent communication skills and the ability to work in a high volume environment is required. To apply, please e-mail resume to: amartine@carisdx.com Angela Martinez Human Resources Coordinator Caris Diagnostics 8400 Esters Blvd. Suite 190 Irving, TX 75063 214-277-8700 Phone 214-596-7490- Fax 214-538-6235 Cell ------------------------------ Message: 3 Date: Mon, 19 Mar 2007 13:41:37 -0400 From: "Patricia Karlisch" Subject: [Histonet] Unsubcribe To: Message-ID: <45FE9311.D9B9.008C.0@psu.edu> Content-Type: text/plain; charset=US-ASCII Please unsubscribe due to vacation. Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 40, Issue 27 **************************************** From tim.morken <@t> thermofisher.com Mon Mar 19 13:57:35 2007 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Mon Mar 19 13:56:58 2007 Subject: [Histonet] Two IHC QC Positions, ThermoFisher Scientific, Lab Vision Message-ID: IHC QC Position, ThermoFisher Scientific, Lab Vision ThermoFisher Scientific / Lab Vision has openings for bench- and senior-level technologists in the IHC quality control laboratory. The lab is new and very pleasant to work in. A relocation package is available. Department: Quality Control - IHC Lab Quality Control Research Associate I and II (I is bench level, II is Senior Technologist level) Summary of Job: Performs quality control tests on antibodies, detection systems and ancillary products used for immunohistochemistry. Performs IHC and works with Technical Support to test retainers and returned lots of possible nonconforming products. Performs sectioning of paraffin tissue blocks and frozen tissue blocks. May participate in special projects involving development of new products, either reagent or instrumentation. Major Responsibilities: Performs routine IHC testing of raw materials and manufactured antibody products according to established quality control procedures/permanent work instructions. Performs stability tests. Releases product as directed by QC IHC Lab Manager. Performs experiments to assess product or procedural problems under direction of QC IHC Lab Manager and/or Technical Support Representative. Maintains and manufactures positive control slide product inventory. Prepares ancillary reagent/buffer products for IHC lab. Performs sectioning of paraffin tissue blocks and frozen tissue blocks. Assists in maintenance of tissue control stock. May be involved in special projects including new product development, product optimization, and optimization requested by sales rep/customers, either reagent or instrumentation. Prepares reports on assigned assays, processes, or quality control tests. Complies with GMP, QSRs, and ISO regulations. Assists in maintaining laboratory equipment to assure accuracy and confidence in performance, and reports equipment problems to Calibration Representative. Complies with ThermoFisher Scientifc's Quality Policies and Procedures. Able to work closely with other departments in reaching company goals. Education or Equivalence of Experience: IHC QC RA I: AA/AS and 1 or more years relevant experience, IHC QC RA II: BA/BS in Chemistry, Biochemistry, Biology, or Bioscience with 3 or more years relevant experience. Both: Able to perform all specific laboratory procedures as requested by the QC IHC Lab Manager. Ability to meet deadlines and ensure successful product release in a timely manner. Must be able to write clear and understandable documentation. Good word processing and spreadsheet skills. Familiar with antibody technology, especially a good understanding of proteins, enzymes, and the structures and functions of antibodies. Able to work in a team environment. IHC QC RA I: Certification as Histotechician (HT) and Qualification for Immunohistochemistry (QIHC) by American Society for Clinical Pathology (ASCP) is desirable. IHC QC RA II: Certification as Histotechnologhist (HTL) and Qualification for Immunohistochemistry (QIHC) by American Society for Clinical Pathology (ASCP) is desirable. Supervisory Responsibilities: IHC QC RA II may oversee work of IHC QC Research Associate I. Please send resumes to: Tim Morken Technical Support Manager Lab Vision - Neomarkers ThermoFisher Scientific 47777 Warm Springs Blvd. Fremont, CA 94539 USA tel: 01-510-991-2840 fax: 01 510-991-2826 Tim.Morken@thermofisher.com web: www.labvision.com www.thermofisher.com From bhewlett <@t> cogeco.ca Mon Mar 19 14:38:40 2007 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Mon Mar 19 14:38:46 2007 Subject: [Histonet] RE: Histonet Digest, Vol 40, Issue 27 Gill's Haemaqtoxylin References: <32833121.48111174330268088.JavaMail.www@wwinf3101> Message-ID: <00fd01c76a5e$325accb0$6500a8c0@mainbox> Duncan, To make Harris' hematoxylin mercury free you only need to substitute 0.5 grams of sodium iodate for the 2.5 grams of mercuric oxide in the existing formula. This is sufficient to oxidize the 5.0 grams of hematoxylin present and will result in a longer useful shelf life. ( See Lillie's text) If you increase the hematoxylin content by 50% you will have to increase the amount of oxidant and will also dramatically alter the dye/mordant ratio. The resulting solution will not stain like Harris', instead it will have a shorter staining time, or require more differentiation and have a reduced shelf life since it will rapidly precipitate the dye lake. Bryan ----- Original Message ----- From: "Duncan Wood" To: Sent: Monday, March 19, 2007 2:51 PM Subject: [Histonet] RE: Histonet Digest, Vol 40,Issue 27 Gill's Haemaqtoxylin If you want to use Gill's then you must use the Aluminium Sulphate, If you want to use the higher strengths of Gill's (double and triple strengths) then make sure that ALL the proportions are done properly, other wise it won't work. It is possible to use Harris's that is mercury free if you are happier with that, but the Haematoxylin content needs to be increased by about 50%. can give you fuller details if you need them. Good staining! Duncan Wood, Consultant, Clin-Tech Ltd., UK ======================================== Message Received: Mar 19 2007, 06:01 PM From: histonet-request@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Cc: Subject: Histonet Digest, Vol 40, Issue 27 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Gill's hematoxylin (Wurdak, Elizabeth) 2. Opportunity (Martinez, Angela) 3. Unsubcribe (Patricia Karlisch) ---------------------------------------------------------------------- Message: 1 Date: Mon, 19 Mar 2007 12:35:52 -0500 From: "Wurdak, Elizabeth" Subject: [Histonet] Gill's hematoxylin To: Histonet Message-ID: Content-Type: text/plain; charset="ISO-8859-1" Hello Histonetters, We have decided to switch from Harris' hematoxylin to Gill's to avoid using mercury. I have a couple of recipes for Gill's hematoxylin. They both call for aluminum sulfate and sodium iodate. I have only ammonia alum, NH4Al(SO4)2x12H2O, or potassium alum, KAl(SO4)2x12H2O and sodium periodate on hand. Can I use these reagents instead and do I need to modify the quantities of each. The IHC World recipe requires 0.2g sodium iodate and 20g aluminum sulfate for 1020 ml of the single strength hematoxylin (2g) solution. Thanks for any help you can give me. Liz Elizabeth Wurdak, PhD Biology Department Saint John?s University Collegeville, MN 56321 Tel: (320) 363-3177 On 3/19/07 8:58 AM, "SHargrove@urhcs.org" wrote: > Regarding the processing of prostate biopsies, We have a "short run" on > one > of the VIP's that we use for all biopsies. Our alcohols are graded from > 70% > - 100%. We used to use sponges for ours and the pathologist thought it > caused some breaking. Imagine the tissue bouncing off of the pores of the > sponge. Now all needle biopsies are wrapped in paper before processing. We > are having much better results .We still use sponges for biopsies. Be sure > the sponges are saturated with formalin before use, and they will not > float. > Susie Hargrove > Tech Specialist Histology > United Regional > Wichita Falls, Texas > > ********************************************************************** > The documents inside this electronic transmission contain > confidential information belonging to the sender that is legally > privileged. This information is intended only for the use of the > individual or entity named above. The authorized recipient of this > information is prohibited from disclosing this information to any > other party and is required to destroy the information after its > stated need has been fulfilled, unless otherwise required by law. > > If you are not the intended recipient, you are hereby notified that > any disclosure, copying, distribution, or action taken in reliance > on the contents of these documents is strictly prohibited. If you > received this electronic transmission in error, please notify the > sender immediately to arrange for return. > ********************************************************************** > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Mon, 19 Mar 2007 12:40:19 -0500 From: "Martinez, Angela" Subject: [Histonet] Opportunity To: Message-ID: <9B8A3AC772C7F64680392A7CB8FBFB0F01CFD9B1@s-irv-ex301.PathologyPartners.intranet> Content-Type: text/plain; charset="us-ascii" Caris would like to announce a new job opening in the Phoenix Arizona location. The position is for a full time Histotechnologist. This position will entail routine Histology including: specimen processing, embedding, microtomy, staining, and immunohistochemistry. Candidates should be HT certified and should possess a BS degree. Further, candidates should have 1-3 years experience in embedding and sectioning of surgical specimens in a certified Histopathology laboratory. Excellent communication skills and the ability to work in a high volume environment is required. To apply, please e-mail resume to: amartine@carisdx.com Angela Martinez Human Resources Coordinator Caris Diagnostics 8400 Esters Blvd. Suite 190 Irving, TX 75063 214-277-8700 Phone 214-596-7490- Fax 214-538-6235 Cell ------------------------------ Message: 3 Date: Mon, 19 Mar 2007 13:41:37 -0400 From: "Patricia Karlisch" Subject: [Histonet] Unsubcribe To: Message-ID: <45FE9311.D9B9.008C.0@psu.edu> Content-Type: text/plain; charset=US-ASCII Please unsubscribe due to vacation. Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 40, Issue 27 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NMargaryan <@t> childrensmemorial.org Mon Mar 19 15:25:11 2007 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Mon Mar 19 15:24:07 2007 Subject: [Histonet] (no subject) Message-ID: Hi Dears, One of my friends is looking the "protocol of quantitative receptor autoradiography method" for sections. Any suggestion is appreciated, Naira Naira V. Margaryan, D.V.M., Ph.D. Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3394 Tel: 773-755-6570/ext-8 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 From SecrestK <@t> wvuh.com Mon Mar 19 15:36:03 2007 From: SecrestK <@t> wvuh.com (Secrest, Kimberly) Date: Mon Mar 19 15:36:28 2007 Subject: [Histonet] Intranuclear inclusions Message-ID: <7708E0A8E46BDC40B23113F97FB0FB23031DCF0A@nt-exchange1.wvuh.wvuhs.com> Hi all, I have a pathologist who has noticed and an unusual prevelence of intranuclear inclusions in our routine H+E slides. She's noticed it in tissue ranging from a liver biopsy to a skin biopsy. Has anyone heard of this occuring regularly and what could cause it? Kimberly Secrest HTL, QIHC Histology Technical Specialist West Virginia University Hospital From jflinn <@t> gmu.edu Mon Mar 19 15:46:59 2007 From: jflinn <@t> gmu.edu (Jane M Flinn) Date: Mon Mar 19 15:47:07 2007 Subject: [Histonet] cryostat purchase Message-ID: We are considering buying a new cryostat and have considered Leica and Vibratome. We need to section mouse and rat brains, we would like a permanent blade, and like digital controls. Does anyone have suggestions? jane "Life is short - make haste to be kind" Dr. Jane Flinn Director, Biopsychology Program George Mason University, 3F5 4400 University Dr. Fairfax, VA 22030 Phone: 703-993-4107 Fax: 703-993-1359 From LuckG <@t> empirehealth.org Mon Mar 19 16:16:55 2007 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Mon Mar 19 16:16:59 2007 Subject: [Histonet] CD138 (Clone B-B4) Message-ID: <6BB8BC4519AAB844B174FC739A679BBCCEFD0E@IRMEXCH01.irm.inhs.org> Hello all, I'm looking for a source the the Ab. and clone in my subject line. Our previous vendor (Serotec) informed us when we attempted to reorder that it was no longer available from them. Thanks, Greg Greg Luck, B.S., HT(ASCP) Anatomic Pathology Supervisor Deaconess Med Cntr 800 W. 5th Ave Spokane, WA 99204 Offc 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconessmc.org From kappeler <@t> patho.unibe.ch Tue Mar 20 01:17:57 2007 From: kappeler <@t> patho.unibe.ch (Andi Kappeler) Date: Tue Mar 20 01:18:50 2007 Subject: [Histonet] CD138 (Clone B-B4) References: <6BB8BC4519AAB844B174FC739A679BBCCEFD0E@IRMEXCH01.irm.inhs.org> Message-ID: <004301c76ab7$811a1c80$27955c82@patho.unibe.ch> Hi Greg we were confronted with the same problem and have switched now to clone MI15, which gives us comparable results (on human FFPE tissue, mainly bone marrow). MI15 is from Dako, CatNo M7228. We use it at 1.8 ug Ig/ml, HIER in citrate, pH 6.0, pressure cooker. Hope this helps. Andi Kappeler Institute of Pathology, University of Bern, Switzerland ----- Original Message ----- From: "Luck, Greg D." To: Sent: Monday, March 19, 2007 10:16 PM Subject: [Histonet] CD138 (Clone B-B4) Hello all, I'm looking for a source the the Ab. and clone in my subject line. Our previous vendor (Serotec) informed us when we attempted to reorder that it was no longer available from them. Thanks, Greg Greg Luck, B.S., HT(ASCP) Anatomic Pathology Supervisor Deaconess Med Cntr 800 W. 5th Ave Spokane, WA 99204 Offc 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconessmc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From csn1x <@t> udcf.gla.ac.uk Tue Mar 20 03:30:58 2007 From: csn1x <@t> udcf.gla.ac.uk (csn1x@udcf.gla.ac.uk) Date: Tue Mar 20 03:31:04 2007 Subject: [Histonet] Adenovirus In-Reply-To: <45184.12441.qm@web61221.mail.yahoo.com> References: <45184.12441.qm@web61221.mail.yahoo.com> Message-ID: <20070320083058.r9php2m2o4wsgsck@udcf.gla.ac.uk> Hi There, I was wondering if anyone would be able to help. I am looking for some information regarding markers for adenovirus and macrophages on spliced spheroids on formalin fixed paraffin embedded tissue. ---------------------------------------------------------------- This message was sent using IMP, the Internet Messaging Program. From sheila_adey <@t> hotmail.com Tue Mar 20 06:13:07 2007 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Tue Mar 20 06:13:13 2007 Subject: [Histonet] Biogenex i6000 users Message-ID: Hello All, We are new users to the i6000 and we are having difficulty eliminating the excess background from our slides. We are using the super sensitive AEC detection and have increased our peroxide block to 20min. I have requested to try the protein block but I don't have it yet. Any suggestions would be greatly appreciated. Have a great day Sheila Adey HT MLT Port Huron Hospital Michigan _________________________________________________________________ RealLiveMoms: Share your experience with Real Live Moms just like you http://www.reallivemoms.ca/ From GDawson <@t> dynacaremilwaukee.com Tue Mar 20 08:08:57 2007 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Tue Mar 20 08:09:12 2007 Subject: [Histonet] Florida License? In-Reply-To: <816DC61E1730E843A0BCF4CCFA1EFCF308925B@jaxmail.umc.ufl.edu> Message-ID: All, Anyone know how much FL shakes you down for this license and if they charge you yearly so that you can keep it? Just Wondering, Glen Dawson IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Santiago, Jerry Sent: Monday, March 19, 2007 9:48 AM To: godsgalnow@aol.com; indytreegers@sbcglobal.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Florida License? Lyn, With your HT(ASCP) you automatically can apply for a technician license. My recommendation is to apply for a technologist license which can be done by using the following routes: 1) HT(ASCP), 5 years of pertinent laboratory experience & 48 contact hours in advanced techniques such as IHC & Molecular 2) HT(ASCP) & QIHC(ASCP) The state test is no longer available, the only acceptance is the ASCP. Jerry Santiago, BS, HTL(ASCP)QIHC Shands Jacksonville Jacksonville, FL 904-244-6149 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of godsgalnow@aol.com Sent: Monday, March 19, 2007 11:21 AM To: indytreegers@sbcglobal.net; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Florida License? If you have your ASCP, there is not another test to take for Florida, just, CEU classes that you need. Check out the website link below. http://www.doh.state.fl.us/mqa/ Roxanne Soto HT(ASCP)QIHC Laboratory Supervisor Physicians RightPath Tampa, Florida -----Original Message----- From: indytreegers@sbcglobal.net To: histonet@lists.utsouthwestern.edu Sent: Mon, 19 Mar 2007 11:12 AM Subject: [Histonet] Florida License? Hi, Can any of you tell me how the Florida License requirements compare to the HT(ASCP)? I am registered with ASCP, but am curious about the FL testing process. My husband just heard about a remotely possible job opportunity in Melbourne, FL, and I am wondering what it would take for me to, well, basically, get a job down there? Thanks in advance for any information you can give me, Lyn Lyn Treeger, B.S. HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Michelle.Perrins <@t> uct.ac.za Tue Mar 20 07:17:16 2007 From: Michelle.Perrins <@t> uct.ac.za (Michelle Perrins) Date: Tue Mar 20 08:49:17 2007 Subject: [Histonet] Storage of specimens etc Message-ID: <45FFECEA.A704.0070.0@uct.ac.za> Hello I would like some input as to the protocol for the storage of wax blocks, histo slides as well as the actual specimens. Thanks Michelle Forensic Medicine Faculty Health Sciences UCT Cape Town South Africa From Vickroy.Jim <@t> mhsil.com Tue Mar 20 08:51:45 2007 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Tue Mar 20 08:54:20 2007 Subject: [Histonet] thermometer for waterbaths Message-ID: I am wondering if there are any suggestions for waterbath (floatation bath) thermometers. In the old days we had a submersible type but I am wondering if there are some NIST traceable thermometers that are in use. Digital or regular. We haven't decided whether to use one thermometer for all of the baths or have a thermometer at every station. Ideas? Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From JWEEMS <@t> sjha.org Tue Mar 20 08:57:09 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Tue Mar 20 08:57:42 2007 Subject: [Histonet] thermometer for waterbaths In-Reply-To: Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D385F@sjhaexc02.sjha.org> We use water baths with the thermometer in the display... we are lucky. But I didn't take time to ask yesterday, but question to the lab who was cited --- who cited you? Thanks, Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Vickroy, Jim Sent: Tuesday, March 20, 2007 9:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] thermometer for waterbaths I am wondering if there are any suggestions for waterbath (floatation bath) thermometers. In the old days we had a submersible type but I am wondering if there are some NIST traceable thermometers that are in use. Digital or regular. We haven't decided whether to use one thermometer for all of the baths or have a thermometer at every station. Ideas? Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From Eric.C.Kellar <@t> questdiagnostics.com Tue Mar 20 09:08:09 2007 From: Eric.C.Kellar <@t> questdiagnostics.com (Kellar, Eric C) Date: Tue Mar 20 09:08:24 2007 Subject: [Histonet] Florida License? Message-ID: <6843061CE6B98E4B96590D4F299618F801583C11@qdcws0117.us.qdx.com> Glen, Here's the Sunshine State Shakedown... Histologic Technician/Histologic Technologist/Supervisor= $100.00 (application fee and is non-refundable) plus Initial license Fee=$105.00=($205.00 total);bi-annual renewal HT/HTL=$136.00, Supervisor=$160.00, plus continuing education credit requirements in Florida Law, HIV/AIDS and Medical Errors. If you currently hold a license at any level and are adding specialty areas or upgrading to a higher level of licensure, a fee of $130.00 is required. Eric C. Kellar Quest Diagnostics Histology Laboratory Supervisor South Florida -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Tuesday, March 20, 2007 9:09 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Florida License? All, Anyone know how much FL shakes you down for this license and if they charge you yearly so that you can keep it? Just Wondering, Glen Dawson IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Santiago, Jerry Sent: Monday, March 19, 2007 9:48 AM To: godsgalnow@aol.com; indytreegers@sbcglobal.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Florida License? Lyn, With your HT(ASCP) you automatically can apply for a technician license. My recommendation is to apply for a technologist license which can be done by using the following routes: 1) HT(ASCP), 5 years of pertinent laboratory experience & 48 contact hours in advanced techniques such as IHC & Molecular 2) HT(ASCP) & QIHC(ASCP) The state test is no longer available, the only acceptance is the ASCP. Jerry Santiago, BS, HTL(ASCP)QIHC Shands Jacksonville Jacksonville, FL 904-244-6149 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of godsgalnow@aol.com Sent: Monday, March 19, 2007 11:21 AM To: indytreegers@sbcglobal.net; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Florida License? If you have your ASCP, there is not another test to take for Florida, just, CEU classes that you need. Check out the website link below. http://www.doh.state.fl.us/mqa/ Roxanne Soto HT(ASCP)QIHC Laboratory Supervisor Physicians RightPath Tampa, Florida -----Original Message----- From: indytreegers@sbcglobal.net To: histonet@lists.utsouthwestern.edu Sent: Mon, 19 Mar 2007 11:12 AM Subject: [Histonet] Florida License? Hi, Can any of you tell me how the Florida License requirements compare to the HT(ASCP)? I am registered with ASCP, but am curious about the FL testing process. My husband just heard about a remotely possible job opportunity in Melbourne, FL, and I am wondering what it would take for me to, well, basically, get a job down there? Thanks in advance for any information you can give me, Lyn Lyn Treeger, B.S. HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LINDA.MARGRAF <@t> childrens.com Tue Mar 20 09:40:32 2007 From: LINDA.MARGRAF <@t> childrens.com (LINDA MARGRAF) Date: Tue Mar 20 09:40:56 2007 Subject: [Histonet] job posting San Antonio Message-ID: <45FFAC10020000DA000081D0@CNET3.CHILDRENS.COM> Research Histotechnologist - San Antonio The Texas Research Park is a component of the University of Texas Health Science Center located on highway 211 to the west of San Antonio. There is a histology core here at the Park currently run by a senior histotechnologist, who will be leaving soon to move away from San Antonio. Therefore we are searching for a replacement. The job may be quite different from what you may be used to, if you now work as part of a large clinical histology lab. As the person running the Histology Core at the Research Park you are more or less "your own boss" because you can arrange your work hours to suit your own schedule. The work is quite varied, involving many new procedures as well as immunohistochemistry. The person we are looking for is independent, self-organized, and a good troubleshooter. Direct experience with new and experimental techniques is not a prerequisite. Our current histotechnologist has generously offered to stay here longer for a brief period to train her replacement. Please go to the web site www.uthscsajobs.com and do "search postings" -- leave "job category" as "any" and use the pull down menu under "job title" to look for "Histotechnologist IV." If you think you don't qualify for the IV level we can adjust the position according to your requirements. Also the salary is negotiable. If I can answer any questions please ask; if you would like to visit us in the Research Park and look at the facilities please send an email to the address below and I will set up a visit. Peter J. Hornsby, Ph.D. Professor, Department of Physiology Sam and Ann Barshop Institute for Longevity and Aging Studies University of Texas Health Science Center 15355 Lambda Drive, STCBM Bldg. San Antonio, TX 78245 web site: http://physiology.uthscsa.edu/research/faculty_view.asp?id=9 e-mail: hornsby@uthscsa.edu phone: 210-562-5080 fax: 281-582-3538 From rjbuesa <@t> yahoo.com Tue Mar 20 10:15:03 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 20 10:15:11 2007 Subject: [Histonet] Intranuclear inclusions In-Reply-To: <7708E0A8E46BDC40B23113F97FB0FB23031DCF0A@nt-exchange1.wvuh.wvuhs.com> Message-ID: <585312.99724.qm@web61219.mail.yahoo.com> A change in the "apparent" presence of intraNUCLEAR inclusions can be the result of a change in the fixative or fixation protocol that could determine a change in the nuclear components precipitation. Have you had a change in fixation or fixative? That could be the explanation. Ren? J. "Secrest, Kimberly" wrote: Hi all, I have a pathologist who has noticed and an unusual prevelence of intranuclear inclusions in our routine H+E slides. She's noticed it in tissue ranging from a liver biopsy to a skin biopsy. Has anyone heard of this occuring regularly and what could cause it? Kimberly Secrest HTL, QIHC Histology Technical Specialist West Virginia University Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Need Mail bonding? Go to the Yahoo! Mail Q&A for great tips from Yahoo! Answers users. From ryakay <@t> shands.ufl.edu Tue Mar 20 10:23:08 2007 From: ryakay <@t> shands.ufl.edu (Kaye Ryan) Date: Tue Mar 20 10:23:35 2007 Subject: [Histonet] thermometer for waterbaths In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D385F@sjhaexc02.sjha.org> References: < 1CD6831EB9B26D45B0A3EAA79F7EBD32037D385F@sjhaexc02.sjha.org> Message-ID: <45FFC41C020000D800002A59@gw-fs1.shands.ufl.edu> Just a question....does no one besides me find it strange that they can cite for this when it has not been in the checklist for several years now? Kaye Kaye Ryan Histology Manager/Educational Coordinator Shands Rocky Point Laboratories (352) 265-0111, 72093 >>> "Weems, Joyce" 3/20/2007 9:57 AM >>> We use water baths with the thermometer in the display... we are lucky. But I didn't take time to ask yesterday, but question to the lab who was cited --- who cited you? Thanks, Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Vickroy, Jim Sent: Tuesday, March 20, 2007 9:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] thermometer for waterbaths I am wondering if there are any suggestions for waterbath (floatation bath) thermometers. In the old days we had a submersible type but I am wondering if there are some NIST traceable thermometers that are in use. Digital or regular. We haven't decided whether to use one thermometer for all of the baths or have a thermometer at every station. Ideas? Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From JWEEMS <@t> sjha.org Tue Mar 20 10:25:54 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Tue Mar 20 10:26:41 2007 Subject: [Histonet] thermometer for waterbaths In-Reply-To: <45FFC41C020000D800002A59@gw-fs1.shands.ufl.edu> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D3865@sjhaexc02.sjha.org> That was my question exactly - who cited them? Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: Kaye Ryan [mailto:ryakay@shands.ufl.edu] Sent: Tuesday, March 20, 2007 11:23 AM To: histonet@lists.utsouthwestern.edu; Jim Vickroy; Weems, Joyce Subject: RE: [Histonet] thermometer for waterbaths Just a question....does no one besides me find it strange that they can cite for this when it has not been in the checklist for several years now? Kaye Kaye Ryan Histology Manager/Educational Coordinator Shands Rocky Point Laboratories (352) 265-0111, 72093 >>> "Weems, Joyce" 3/20/2007 9:57 AM >>> We use water baths with the thermometer in the display... we are lucky. But I didn't take time to ask yesterday, but question to the lab who was cited --- who cited you? Thanks, Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Vickroy, Jim Sent: Tuesday, March 20, 2007 9:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] thermometer for waterbaths I am wondering if there are any suggestions for waterbath (floatation bath) thermometers. In the old days we had a submersible type but I am wondering if there are some NIST traceable thermometers that are in use. Digital or regular. We haven't decided whether to use one thermometer for all of the baths or have a thermometer at every station. Ideas? Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From HoustonR <@t> chi.osu.edu Tue Mar 20 11:07:43 2007 From: HoustonR <@t> chi.osu.edu (Houston, Ronald) Date: Tue Mar 20 11:08:13 2007 Subject: [Histonet] vented storage space Message-ID: <979FF5962E234F45B06CF0DB7C1AABB20D77D0B8@chi2k3ms01.columbuschildrens.net> I know it's a bit like "how long is a piece of string?" but would anyone have any idea of recommendations for adequate air exchange in a formaldehyde specimen storage room? Thanks Ronnie Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Columbus Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 houstonr@chi.osu.edu ----------------------------------------- Confidentiality Notice: This e-mail message, from Children's Hospital, Columbus, Ohio, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes pursuant to Children's Hospital's confidentiality policies. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From Terry.Marshall <@t> rothgen.nhs.uk Tue Mar 20 11:19:29 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Tue Mar 20 11:19:39 2007 Subject: [Histonet] RE: Histonet Digest, Vol 40, Issue 27 Gill's Haemaqtoxylin Message-ID: <407F05A128805F4C879A33DBA32E618E20C28B@TRFT-EX01.xRothGen.nhs.uk> But why stop there instead of the real thing? The antifreeze stops the precipitation of haematin in Gill's. Isn't it everyone's dream to make it last longer:-) Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bryan Hewlett Sent: 19 March 2007 19:39 To: duncanwood1@fsmail.net; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Histonet Digest, Vol 40,Issue 27 Gill's Haemaqtoxylin Duncan, To make Harris' hematoxylin mercury free you only need to substitute 0.5 grams of sodium iodate for the 2.5 grams of mercuric oxide in the existing formula. This is sufficient to oxidize the 5.0 grams of hematoxylin present and will result in a longer useful shelf life. ( See Lillie's text) If you increase the hematoxylin content by 50% you will have to increase the amount of oxidant and will also dramatically alter the dye/mordant ratio. The resulting solution will not stain like Harris', instead it will have a shorter staining time, or require more differentiation and have a reduced shelf life since it will rapidly precipitate the dye lake. Bryan ----- Original Message ----- From: "Duncan Wood" To: Sent: Monday, March 19, 2007 2:51 PM Subject: [Histonet] RE: Histonet Digest, Vol 40,Issue 27 Gill's Haemaqtoxylin If you want to use Gill's then you must use the Aluminium Sulphate, If you want to use the higher strengths of Gill's (double and triple strengths) then make sure that ALL the proportions are done properly, other wise it won't work. It is possible to use Harris's that is mercury free if you are happier with that, but the Haematoxylin content needs to be increased by about 50%. can give you fuller details if you need them. Good staining! Duncan Wood, Consultant, Clin-Tech Ltd., UK ======================================== Message Received: Mar 19 2007, 06:01 PM From: histonet-request@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Cc: Subject: Histonet Digest, Vol 40, Issue 27 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Gill's hematoxylin (Wurdak, Elizabeth) 2. Opportunity (Martinez, Angela) 3. Unsubcribe (Patricia Karlisch) ---------------------------------------------------------------------- Message: 1 Date: Mon, 19 Mar 2007 12:35:52 -0500 From: "Wurdak, Elizabeth" Subject: [Histonet] Gill's hematoxylin To: Histonet Message-ID: Content-Type: text/plain; charset="ISO-8859-1" Hello Histonetters, We have decided to switch from Harris' hematoxylin to Gill's to avoid using mercury. I have a couple of recipes for Gill's hematoxylin. They both call for aluminum sulfate and sodium iodate. I have only ammonia alum, NH4Al(SO4)2x12H2O, or potassium alum, KAl(SO4)2x12H2O and sodium periodate on hand. Can I use these reagents instead and do I need to modify the quantities of each. The IHC World recipe requires 0.2g sodium iodate and 20g aluminum sulfate for 1020 ml of the single strength hematoxylin (2g) solution. Thanks for any help you can give me. Liz Elizabeth Wurdak, PhD Biology Department Saint John?s University Collegeville, MN 56321 Tel: (320) 363-3177 On 3/19/07 8:58 AM, "SHargrove@urhcs.org" wrote: > Regarding the processing of prostate biopsies, We have a "short run" on > one > of the VIP's that we use for all biopsies. Our alcohols are graded from > 70% > - 100%. We used to use sponges for ours and the pathologist thought it > caused some breaking. Imagine the tissue bouncing off of the pores of the > sponge. Now all needle biopsies are wrapped in paper before processing. We > are having much better results .We still use sponges for biopsies. Be sure > the sponges are saturated with formalin before use, and they will not > float. > Susie Hargrove > Tech Specialist Histology > United Regional > Wichita Falls, Texas > > ********************************************************************** > The documents inside this electronic transmission contain > confidential information belonging to the sender that is legally > privileged. This information is intended only for the use of the > individual or entity named above. The authorized recipient of this > information is prohibited from disclosing this information to any > other party and is required to destroy the information after its > stated need has been fulfilled, unless otherwise required by law. > > If you are not the intended recipient, you are hereby notified that > any disclosure, copying, distribution, or action taken in reliance > on the contents of these documents is strictly prohibited. If you > received this electronic transmission in error, please notify the > sender immediately to arrange for return. > ********************************************************************** > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Mon, 19 Mar 2007 12:40:19 -0500 From: "Martinez, Angela" Subject: [Histonet] Opportunity To: Message-ID: <9B8A3AC772C7F64680392A7CB8FBFB0F01CFD9B1@s-irv-ex301.PathologyPartners.intranet> Content-Type: text/plain; charset="us-ascii" Caris would like to announce a new job opening in the Phoenix Arizona location. The position is for a full time Histotechnologist. This position will entail routine Histology including: specimen processing, embedding, microtomy, staining, and immunohistochemistry. Candidates should be HT certified and should possess a BS degree. Further, candidates should have 1-3 years experience in embedding and sectioning of surgical specimens in a certified Histopathology laboratory. Excellent communication skills and the ability to work in a high volume environment is required. To apply, please e-mail resume to: amartine@carisdx.com Angela Martinez Human Resources Coordinator Caris Diagnostics 8400 Esters Blvd. Suite 190 Irving, TX 75063 214-277-8700 Phone 214-596-7490- Fax 214-538-6235 Cell ------------------------------ Message: 3 Date: Mon, 19 Mar 2007 13:41:37 -0400 From: "Patricia Karlisch" Subject: [Histonet] Unsubcribe To: Message-ID: <45FE9311.D9B9.008C.0@psu.edu> Content-Type: text/plain; charset=US-ASCII Please unsubscribe due to vacation. Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 40, Issue 27 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Tue Mar 20 11:22:09 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Tue Mar 20 11:22:23 2007 Subject: [Histonet] thermometer for waterbaths Message-ID: <407F05A128805F4C879A33DBA32E618E20C28C@TRFT-EX01.xRothGen.nhs.uk> Odd that this subject came up now, because I had to come in at the weekend, and went into the lab and looked at the waterbaths which were still on. One had the thermostat (joke) set at 40 and the temperature from a thermometer was 48. The other had the temperature set at 50, and the temperature was 56. And you lot have the cheek to make digs about the pathologists!!! Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: 20 March 2007 13:52 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] thermometer for waterbaths I am wondering if there are any suggestions for waterbath (floatation bath) thermometers. In the old days we had a submersible type but I am wondering if there are some NIST traceable thermometers that are in use. Digital or regular. We haven't decided whether to use one thermometer for all of the baths or have a thermometer at every station. Ideas? Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Tue Mar 20 11:30:31 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Mar 20 11:30:44 2007 Subject: [Histonet] thermometer for waterbaths Message-ID: <86ADE4EB583CE64799A9924684A0FBBF012473F7@wahtntex2.waht.swest.nhs.uk> Odd that this subject came up now, because I had to come in at the weekend, and went into the lab and looked at the waterbaths which were still on. One had the thermostat (joke) set at 40 and the temperature from a thermometer was 48. The other had the temperature set at 50, and the temperature was 56. And you lot have the cheek to make digs about the pathologists!!! Terry So what was the temperature of the water then? Bet you stuck the thermometer into the water touching the bottom didn't you? Don't you know that the heating element will cause the sides to be warmer than the water? You need to take the temperature by dangling the thermometer into the water but not touching the sides or bottom. Easy mistake for a Pathologist. Kemlo Rogerson Pathology Manager This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From ree3 <@t> leicester.ac.uk Tue Mar 20 11:35:23 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Tue Mar 20 11:35:40 2007 Subject: [Histonet] thermometer for waterbaths In-Reply-To: <407F05A128805F4C879A33DBA32E618E20C28C@TRFT-EX01.xRothGen.nhs.uk> References: <407F05A128805F4C879A33DBA32E618E20C28C@TRFT-EX01.xRothGen.nhs.uk> Message-ID: Well what sort of pathologist has the time or might even want to take the temperature of water baths at the weekend, so do you also go through all the drawers and cupboards in the lab when nobody is there, looking for things to criticise the lab staff on when they return on a Monday?? dearie me, what has the world come to?. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: 20 March 2007 16:22 To: Vickroy, Jim; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] thermometer for waterbaths Odd that this subject came up now, because I had to come in at the weekend, and went into the lab and looked at the waterbaths which were still on. One had the thermostat (joke) set at 40 and the temperature from a thermometer was 48. The other had the temperature set at 50, and the temperature was 56. And you lot have the cheek to make digs about the pathologists!!! Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: 20 March 2007 13:52 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] thermometer for waterbaths I am wondering if there are any suggestions for waterbath (floatation bath) thermometers. In the old days we had a submersible type but I am wondering if there are some NIST traceable thermometers that are in use. Digital or regular. We haven't decided whether to use one thermometer for all of the baths or have a thermometer at every station. Ideas? Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Tue Mar 20 11:37:49 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Tue Mar 20 11:38:05 2007 Subject: [Histonet] thermometer for waterbaths Message-ID: <407F05A128805F4C879A33DBA32E618E20C28D@TRFT-EX01.xRothGen.nhs.uk> Wrong Kemlo, I am not that stupid even though I am only a pathologist. I took the temperature in the middle and midway between the "floor" and water surface. Terry ________________________________ From: Kemlo Rogerson [mailto:Kemlo.Rogerson@waht.swest.nhs.uk] Sent: 20 March 2007 16:31 To: Marshall Terry Dr, Consultant Histopathologist; Vickroy, Jim; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] thermometer for waterbaths Odd that this subject came up now, because I had to come in at the weekend, and went into the lab and looked at the waterbaths which were still on. One had the thermostat (joke) set at 40 and the temperature from a thermometer was 48. The other had the temperature set at 50, and the temperature was 56. And you lot have the cheek to make digs about the pathologists!!! Terry So what was the temperature of the water then? Bet you stuck the thermometer into the water touching the bottom didn't you? Don't you know that the heating element will cause the sides to be warmer than the water? You need to take the temperature by dangling the thermometer into the water but not touching the sides or bottom. Easy mistake for a Pathologist. Kemlo Rogerson Pathology Manager This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From relia1 <@t> earthlink.net Tue Mar 20 11:38:31 2007 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Mar 20 11:38:38 2007 Subject: [Histonet] RELIA Job Alert - IHC Manager - California Message-ID: Hi Histonetters, I have an exciting opportunity for an experienced Immunohistochemistry Manager with a prestigious company located in California. The compensation package is excellent, the work is challenging and the sky is the limit. The position is of course full time and day shift Monday ? Friday. If you would like more information or know of someone else who might be interested, please contact me at relia1@earthlink.net or 866-607-3542. I am available to discuss the opportunity at your convenience including after hours. Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net From Terry.Marshall <@t> rothgen.nhs.uk Tue Mar 20 11:44:33 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Tue Mar 20 11:44:59 2007 Subject: [Histonet] thermometer for waterbaths Message-ID: <407F05A128805F4C879A33DBA32E618E20C28E@TRFT-EX01.xRothGen.nhs.uk> The sort that has to put up with "exploded" sections. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, R.E. Sent: 20 March 2007 16:35 To: Marshall Terry Dr, Consultant Histopathologist; Vickroy, Jim; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] thermometer for waterbaths Well what sort of pathologist has the time or might even want to take the temperature of water baths at the weekend, so do you also go through all the drawers and cupboards in the lab when nobody is there, looking for things to criticise the lab staff on when they return on a Monday?? dearie me, what has the world come to?. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: 20 March 2007 16:22 To: Vickroy, Jim; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] thermometer for waterbaths Odd that this subject came up now, because I had to come in at the weekend, and went into the lab and looked at the waterbaths which were still on. One had the thermostat (joke) set at 40 and the temperature from a thermometer was 48. The other had the temperature set at 50, and the temperature was 56. And you lot have the cheek to make digs about the pathologists!!! Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: 20 March 2007 13:52 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] thermometer for waterbaths I am wondering if there are any suggestions for waterbath (floatation bath) thermometers. In the old days we had a submersible type but I am wondering if there are some NIST traceable thermometers that are in use. Digital or regular. We haven't decided whether to use one thermometer for all of the baths or have a thermometer at every station. Ideas? Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Tue Mar 20 11:47:31 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Tue Mar 20 11:47:53 2007 Subject: [Histonet] thermometer for waterbaths In-Reply-To: <407F05A128805F4C879A33DBA32E618E20C28E@TRFT-EX01.xRothGen.nhs.uk> References: <407F05A128805F4C879A33DBA32E618E20C28E@TRFT-EX01.xRothGen.nhs.uk> Message-ID: Well you shouldn't have to, you are the boss, get them to buck up there ideas, called good management. -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: 20 March 2007 16:45 To: Edwards, R.E.; Vickroy, Jim; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] thermometer for waterbaths The sort that has to put up with "exploded" sections. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, R.E. Sent: 20 March 2007 16:35 To: Marshall Terry Dr, Consultant Histopathologist; Vickroy, Jim; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] thermometer for waterbaths Well what sort of pathologist has the time or might even want to take the temperature of water baths at the weekend, so do you also go through all the drawers and cupboards in the lab when nobody is there, looking for things to criticise the lab staff on when they return on a Monday?? dearie me, what has the world come to?. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: 20 March 2007 16:22 To: Vickroy, Jim; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] thermometer for waterbaths Odd that this subject came up now, because I had to come in at the weekend, and went into the lab and looked at the waterbaths which were still on. One had the thermostat (joke) set at 40 and the temperature from a thermometer was 48. The other had the temperature set at 50, and the temperature was 56. And you lot have the cheek to make digs about the pathologists!!! Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: 20 March 2007 13:52 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] thermometer for waterbaths I am wondering if there are any suggestions for waterbath (floatation bath) thermometers. In the old days we had a submersible type but I am wondering if there are some NIST traceable thermometers that are in use. Digital or regular. We haven't decided whether to use one thermometer for all of the baths or have a thermometer at every station. Ideas? Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kbowden <@t> ucsd.edu Tue Mar 20 11:53:10 2007 From: kbowden <@t> ucsd.edu (kbowden) Date: Tue Mar 20 11:53:34 2007 Subject: [Histonet] thermometer for waterbaths In-Reply-To: References: Message-ID: <46001176.40408@ucsd.edu> What did I miss? I didn't see anything about being cited. When did the war start between pathologists and histologists? Aren't we all on the same team? I use an external digital thermometer that has a probe attached. I got it from Fishersci.com Karen Bowden Staff Research Associate II University of CA, San Diego 9500 Gilman Dr. 0630 La Jolla, CA 92093-0630 858-534-4655 Voice 858-534-5304 Fax kbowden@ucsd.edu CONFIDENTIALITY NOTICE: THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED. IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT THE SENDER ANDDELETE THE MATERIAL FROM ANY COMPUTER. Vickroy, Jim wrote: > I am wondering if there are any suggestions for waterbath (floatation > bath) thermometers. In the old days we had a submersible type but I am > wondering if there are some NIST traceable thermometers that are in use. > Digital or regular. We haven't decided whether to use one thermometer > for all of the baths or have a thermometer at every station. Ideas? > > > > > > > > Jim Vickroy > > Technical Supervisor - Surgical and Autopsy Pathology > > Memorial Medical Center > > > > > > This message (including any attachments) contains confidential information intended for a > specific individual and purpose, and is protected by law. If you are not the intended recipient, > you should delete this message. Any disclosure, copying, or distribution of this message, or the > taking of any action based on it, is strictly prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Jacqueline.Malam <@t> rli.mbht.nhs.uk Tue Mar 20 11:52:28 2007 From: Jacqueline.Malam <@t> rli.mbht.nhs.uk (Malam Jacqueline) Date: Tue Mar 20 11:54:54 2007 Subject: [Histonet] RE: sectioning spleen Message-ID: When sectioning haemorrhagic tissues like spleen that don't "cooperate" and begin to crumble, we use Baker's Fluid - 10% glycerol in 70% industrial methylated spirits (IMS) and soak the surface for 10 minutes then cool and cut. Jacqui DISCLAIMER: This e-mail is confidential and privileged. If you are not the intended recipient please accept our apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform postmaster@rli.mbht.nhs.uk that this message has gone astray before deleting it. Comments or opinions expressed in this email are those of their respective contributors only. The views expressed do not represent the views of the Trust, its management or employees. University Hospitals of Morecambe Bay NHS Trust is not responsible and disclaims any and all liability for the content of comments written within.Thank you for your co-operation. From Terry.Marshall <@t> rothgen.nhs.uk Tue Mar 20 12:04:19 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Tue Mar 20 12:04:54 2007 Subject: [Histonet] thermometer for waterbaths Message-ID: <407F05A128805F4C879A33DBA32E618E20C28F@TRFT-EX01.xRothGen.nhs.uk> This is when the war started:-) Question: Hello, If there was a perfect Histology Lab what would it be like ? Does anyone know if there are plans published for the perfect Histology lab. thanks Answer: one without pathologists. Oops, did I say that out loud? Is it Friday yet? Joe Nocito What did I miss? I didn't see anything about being cited. When did the war start between pathologists and histologists? Aren't we all on the same team? I use an external digital thermometer that has a probe attached. I got it from Fishersci.com Karen Bowden Staff Research Associate II University of CA, San Diego 9500 Gilman Dr. 0630 La Jolla, CA 92093-0630 858-534-4655 Voice 858-534-5304 Fax kbowden@ucsd.edu CONFIDENTIALITY NOTICE: THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED. IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT THE SENDER ANDDELETE THE MATERIAL FROM ANY COMPUTER. Vickroy, Jim wrote: > I am wondering if there are any suggestions for waterbath (floatation > bath) thermometers. In the old days we had a submersible type but I > am wondering if there are some NIST traceable thermometers that are in use. > Digital or regular. We haven't decided whether to use one thermometer > for all of the baths or have a thermometer at every station. Ideas? > > > > > > > > Jim Vickroy > > Technical Supervisor - Surgical and Autopsy Pathology > > Memorial Medical Center > > > > > > This message (including any attachments) contains confidential > information intended for a specific individual and purpose, and is > protected by law. If you are not the intended recipient, you should > delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bakevictoria <@t> gmail.com Tue Mar 20 12:30:05 2007 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Tue Mar 20 12:30:15 2007 Subject: [Histonet] cutting of whole mount prostates Message-ID: <4f016b690703201030s3d2336dbj134871b14ce234f1@mail.gmail.com> Hi Everyone! We received a whole prostate that is as hard as a rock (sorry guys). We have to be sure to get high quality sections on these large blocks. Normally we use an ammonia water type ice bath, but this time it is just not working. Does anyone out there have any suggestions that might help us soften this? Thanks in advance. Vikki Baker From Maxim_71 <@t> mail.ru Tue Mar 20 12:45:15 2007 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Tue Mar 20 12:47:40 2007 Subject: [Histonet] Adenovirus In-Reply-To: <1174400261.1014012607@mx29.mail.ru> References: <1174400261.1014012607@mx29.mail.ru> Message-ID: <1946495037.20070320204515@mail.ru> Hi, unknown histonet subscriber: Many viral inclusion bodies stained with Macchiavello technique for viral inclusions, as some rickettsia and chlamydia are magenta. Maxim Peshkov Taganrog Russia. From JWEEMS <@t> sjha.org Tue Mar 20 12:47:14 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Tue Mar 20 12:48:10 2007 Subject: [Histonet] cutting of whole mount prostates In-Reply-To: <4f016b690703201030s3d2336dbj134871b14ce234f1@mail.gmail.com> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D387C@sjhaexc02.sjha.org> I'd try soap first, and then glycerin or Nair... j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Victoria Baker Sent: Tuesday, March 20, 2007 1:30 PM To: Histo Net list server Subject: [Histonet] cutting of whole mount prostates Hi Everyone! We received a whole prostate that is as hard as a rock (sorry guys). We have to be sure to get high quality sections on these large blocks. Normally we use an ammonia water type ice bath, but this time it is just not working. Does anyone out there have any suggestions that might help us soften this? Thanks in advance. Vikki Baker _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From psicurello <@t> mcvh-vcu.edu Tue Mar 20 13:58:52 2007 From: psicurello <@t> mcvh-vcu.edu (Paula Sicurello) Date: Tue Mar 20 14:00:23 2007 Subject: [Histonet] Stain for fibrin and platelets other than Carstair's Message-ID: Hello Netters, &n Does anyone know of a stain for fibrin and Carstair's? I'm working on a small research cas buy all the nasty, icky ingredients for this stain (lik acid) Let me know if you do, Paula S. VCUHS NOTE: T and confidential message is not the i any dissemination, distribu strictly prohibited. If you have error, please notify us immediately by reply deleting it from your computer. -------------------------------------- VCU Health System http://www.vcuhealth.org From rjbuesa <@t> yahoo.com Tue Mar 20 14:24:43 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 20 14:24:52 2007 Subject: [Histonet] Stain for fibrin and platelets other than Carstair's In-Reply-To: Message-ID: <462184.6901.qm@web61214.mail.yahoo.com> Paula: Fibrin can be demonstrated with the stains developed by Kockel (1899) and Weigert (1887). It can also be detected with any H&E. As for platelets Giemsa for tissue is very good. Ren? J. Paula Sicurello wrote: Hello Netters, &n= bsp; Does anyone know of a stain for fibrin and= platelets that isn't Carstair's? I'm working on a small research cas= e and don't want to buy all the nasty, icky ingredients for this stain (lik= e picric acid) Let me know if you do, Paula S. VCUHS NOTE: T= he information contained in this message may be privileged and confidential= and protected from disclosure. If the reader of this message is not the i= ntended recipient, you are hereby notified that any dissemination, distribu= tion or copying of this communication is strictly prohibited. If you have = received this communication in error, please notify us immediately by reply= ing to the message and deleting it from your computer. -------------------------------------- VCU Health System http://www.vcuhealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Don't be flakey. Get Yahoo! Mail for Mobile and always stay connected to friends. From cbobrowi <@t> mcw.edu Tue Mar 20 15:01:16 2007 From: cbobrowi <@t> mcw.edu (Bobrowitz, Carol) Date: Tue Mar 20 15:01:21 2007 Subject: [Histonet] Kurabo AS-200 Message-ID: <8F78639AC56F4143B267FE5F5A1B92C8F0ADED@guyton.phys.mcw.edu> Hi, Has anyone seen the Auto Slide Preparation System AS-200? It can replaces a Histotech! It can cut and stain. The company name is Kurabo Bio-Medical. http://www.bio.kurabo.co.jp/English/as200.html Bye, Bye, Carol Ann Bobrowitz MCW From jjenkins <@t> sch-farmville.org Tue Mar 20 15:27:30 2007 From: jjenkins <@t> sch-farmville.org (Jennie Jenkins) Date: Tue Mar 20 15:12:14 2007 Subject: [Histonet] Does anyone know where I might find... Message-ID: <460043B2.5090709@sch-farmville.org> ...microwave-safe slide stainer racks that fit a Shandon Varistain XY? We would like to be able to microwave the slides for a few minutes before placing them in the section dryer. Thanks in advance. Jennie Jenkins, MT, HT Southside Community Hospital Farmville, Virginia 23901 434-315-2618 ================================================== This e-mail message (and attachments) may contain information that is confidential to Southside Community Hospital. If you are not the intended recipient you cannot use, distribute or copy the message or attachments. In such a case, please notify the sender by return e-mail immediately and erase all copies of the message and attachments. Opinions, conclusions and other information in this message and attachments that do not relate to the official business of Southside Community Hospital are neither given nor endorsed by it. ================================================== From TJasper <@t> smdc.org Tue Mar 20 15:30:25 2007 From: TJasper <@t> smdc.org (Jasper, Thomas G.) Date: Tue Mar 20 15:30:38 2007 Subject: [Histonet] Kurabo AS-200 In-Reply-To: <8F78639AC56F4143B267FE5F5A1B92C8F0ADED@guyton.phys.mcw.edu> Message-ID: <1A9F2A6C5762524799A816F1F09744CF0143A56E@SCREECH.ntcampus.smdc.org> Carol Ann, That's "quite" a claim. Is this cutting (bleeding) edge technology? I've never heard of it, haven't seen it at any conventions or know of any reference from our illustrious, notable and knowledgable histonetters, until now. Maybe I missed something but this seems pretty big, I daresay, revolutionary?! Perhaps you could provide more detail, or maybe someone else out there could? My curiosity and skepticism (I must admit)have been piqued. Thanks, Tom J. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Bobrowitz, Carol Sent: Tuesday, March 20, 2007 3:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kurabo AS-200 Hi, Has anyone seen the Auto Slide Preparation System AS-200? It can replaces a Histotech! It can cut and stain. The company name is Kurabo Bio-Medical. http://www.bio.kurabo.co.jp/English/as200.html Bye, Bye, Carol Ann Bobrowitz MCW _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From jwatson <@t> gnf.org Tue Mar 20 15:41:22 2007 From: jwatson <@t> gnf.org (James Watson) Date: Tue Mar 20 15:41:36 2007 Subject: [Histonet] Kurabo AS-200 In-Reply-To: <1A9F2A6C5762524799A816F1F09744CF0143A56E@SCREECH.ntcampus.smdc.org> Message-ID: Note the disclaimer at the bottom of the add: ***This product is sold not as a medical device, but restricted to research purposes only. Do not use for such purposes as manufacturing and quality control of medical products and also various diagnosises and treatments.*** Only for those of us in research. Please do not let one of these cut through a biopsy of mine. JW -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jasper, Thomas G. Sent: Tuesday, March 20, 2007 1:30 PM To: Bobrowitz, Carol Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Kurabo AS-200 Carol Ann, That's "quite" a claim. Is this cutting (bleeding) edge technology? I've never heard of it, haven't seen it at any conventions or know of any reference from our illustrious, notable and knowledgable histonetters, until now. Maybe I missed something but this seems pretty big, I daresay, revolutionary?! Perhaps you could provide more detail, or maybe someone else out there could? My curiosity and skepticism (I must admit)have been piqued. Thanks, Tom J. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Bobrowitz, Carol Sent: Tuesday, March 20, 2007 3:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kurabo AS-200 Hi, Has anyone seen the Auto Slide Preparation System AS-200? It can replaces a Histotech! It can cut and stain. The company name is Kurabo Bio-Medical. http://www.bio.kurabo.co.jp/English/as200.html Bye, Bye, Carol Ann Bobrowitz MCW _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJasper <@t> smdc.org Tue Mar 20 16:04:16 2007 From: TJasper <@t> smdc.org (Jasper, Thomas G.) Date: Tue Mar 20 16:04:32 2007 Subject: FW: [Histonet] Kurabo AS-200 Message-ID: <1A9F2A6C5762524799A816F1F09744CF0143A571@SCREECH.ntcampus.smdc.org> I misfired sending this the first time, sorry. -----Original Message----- From: Jasper, Thomas G. Sent: Tuesday, March 20, 2007 3:59 PM To: 'James Watson' Cc: 'histonet@lists.utsouthwestern' Subject: RE: [Histonet] Kurabo AS-200 James and all, I just noted the disclaimer at the bottom, interesting. I worked in research for a while and I understand your concern James. Irretrievable specimens exist in all areas of Histology; Research being no exception. I'm not against new technology per se, Lord knows we need it to survive in the world we find ourselves in. This however is somewhat unnerving. I would be very uncomfortable, as we all know, in the clinical world, our blocks and slides are patients...people. Our finest technical pieces of equipment do not always run perfectly. Most of the time this can be addressed by repair, re-work etc. My initial response to the Kurabo AS-200 is one of extreme risk, the kind that makes you a little sick in the pit of your stomach. I'm seeing a lot of red flags here. tj -----Original Message----- From: James Watson [mailto:jwatson@gnf.org] Sent: Tuesday, March 20, 2007 3:41 PM To: Jasper, Thomas G.; Bobrowitz, Carol Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Kurabo AS-200 Note the disclaimer at the bottom of the add: ***This product is sold not as a medical device, but restricted to research purposes only. Do not use for such purposes as manufacturing and quality control of medical products and also various diagnosises and treatments.*** Only for those of us in research. Please do not let one of these cut through a biopsy of mine. JW -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jasper, Thomas G. Sent: Tuesday, March 20, 2007 1:30 PM To: Bobrowitz, Carol Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Kurabo AS-200 Carol Ann, That's "quite" a claim. Is this cutting (bleeding) edge technology? I've never heard of it, haven't seen it at any conventions or know of any reference from our illustrious, notable and knowledgable histonetters, until now. Maybe I missed something but this seems pretty big, I daresay, revolutionary?! Perhaps you could provide more detail, or maybe someone else out there could? My curiosity and skepticism (I must admit)have been piqued. Thanks, Tom J. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Bobrowitz, Carol Sent: Tuesday, March 20, 2007 3:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kurabo AS-200 Hi, Has anyone seen the Auto Slide Preparation System AS-200? It can replaces a Histotech! It can cut and stain. The company name is Kurabo Bio-Medical. http://www.bio.kurabo.co.jp/English/as200.html Bye, Bye, Carol Ann Bobrowitz MCW _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From melissa.mazan <@t> tufts.edu Tue Mar 20 16:26:24 2007 From: melissa.mazan <@t> tufts.edu (Melissa Mazan) Date: Tue Mar 20 16:26:29 2007 Subject: [Histonet] anti-rat sca-1 Message-ID: <20070320172624.7brt2ecz5cs48kw0@webmail.tufts.edu> Hi all, I'm looking for an anti-rat sca-1 (if there is such an analogue to mouse Ly6A/E?) monoclonal - does anyone have any information on this? Thanks in advance - Melissa Melissa R. Mazan, DVM, Diplomate ACVIM Associate Professor and Director of Equine Sports Medicine Department of Clinical Sciences Tufts Cumming School of Veterinary Medicine 200 Westborough Road North Grafton, MA 01536 Tel:508-839-5395 Fax:508-839-7922 email: melissa.mazan@tufts.edu From CIngles <@t> uwhealth.org Tue Mar 20 17:25:45 2007 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Tue Mar 20 17:25:52 2007 Subject: [Histonet] Kurabo AS-200 References: <8F78639AC56F4143B267FE5F5A1B92C8F0ADED@guyton.phys.mcw.edu> Message-ID: <08A0A863637F1349BBFD83A96B27A50A12000F@uwhis-xchng3.uwhis.hosp.wisc.edu> Why would we be interested in something that would replace us? This add makes me feel like some of the guys at the auto production plants when they started to become automated. I am NOT a robot. (and I don't work on unfeeling metal parts). Sorry, I also have a strong aversion to absolutes.(no, not the Vodka) Is it Friday yet? Claire Ingles UW Hospital and Clinics Madison WI ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Bobrowitz, Carol Sent: Tue 3/20/2007 3:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kurabo AS-200 Hi, Has anyone seen the Auto Slide Preparation System AS-200? It can replaces a Histotech! It can cut and stain. The company name is Kurabo Bio-Medical. http://www.bio.kurabo.co.jp/English/as200.html Bye, Bye, Carol Ann Bobrowitz MCW _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From plaurie <@t> benaroyaresearch.org Tue Mar 20 17:42:34 2007 From: plaurie <@t> benaroyaresearch.org (Patrick Laurie) Date: Tue Mar 20 17:42:49 2007 Subject: [Histonet] Kurabo AS-200 Message-ID: <635a348f1ffbc94b9fa69143b55b2720@localhost.localdomain> I completely agree with one of my coworkers, who said that he isn't worried until it can cut one of the difficult decals that he skillfully cut this morning. I, for one, am looking forward to servicing and maintaining our robot overlords. Patrick Laurie, HT (ASCP) Neurogenomics Laboratory Benaroya Research Institute 1201 9th Ave Seattle, Wa 98101 (206) 341-0681 Hi, Has anyone seen the Auto Slide Preparation System AS-200? It can replaces a Histotech! It can cut and stain. The company name is Kurabo Bio-Medical. http://www.bio.kurabo.co.jp/English/as200.html Bye, Bye, Carol Ann Bobrowitz MCW From Jacqueline.Malam <@t> rli.mbht.nhs.uk Wed Mar 21 04:19:53 2007 From: Jacqueline.Malam <@t> rli.mbht.nhs.uk (Malam Jacqueline) Date: Wed Mar 21 04:22:17 2007 Subject: [Histonet] RE: CD 138 Message-ID: We get the B-B4 clone from Lab Vision Jacqui malam Histology Lancaster uk DISCLAIMER: This e-mail is confidential and privileged. If you are not the intended recipient please accept our apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform postmaster@rli.mbht.nhs.uk that this message has gone astray before deleting it. Comments or opinions expressed in this email are those of their respective contributors only. The views expressed do not represent the views of the Trust, its management or employees. University Hospitals of Morecambe Bay NHS Trust is not responsible and disclaims any and all liability for the content of comments written within.Thank you for your co-operation. From Paivi.Parkkisenniemi <@t> lshp.fi Wed Mar 21 04:46:02 2007 From: Paivi.Parkkisenniemi <@t> lshp.fi (Parkkisenniemi Paivi LSHP) Date: Wed Mar 21 04:46:39 2007 Subject: [Histonet] Remove me from histonet email list Message-ID: <6966B0AB401CE845B977613354CEDA91254C77@lap-ex-01.lapitoy.fi> Hello, Please, remove from Histonetters email lists. Thank you. P?ivi From mikael.niku <@t> helsinki.fi Wed Mar 21 05:49:02 2007 From: mikael.niku <@t> helsinki.fi (Mikael Niku) Date: Wed Mar 21 05:49:12 2007 Subject: [Histonet] Problem with intestinal epithelium Message-ID: <46010D9E.7050305@helsinki.fi> Dear Histonetters, we are trying to make good-quality sections (paraffin and cryo) of bovine intestines. The problem is that the epithelium seems to be stripped off whatever we do. This seems to be a problem especially with slaughterhouse material, although the delay from death to sampling is less than 30 min. Currently we are cutting the intestine to pieces, washing with PBS buffer, and fixing immediately in buffered paraformaldehyde or freezing in isopentane / liquid nitrogen. Any ideas, anyone? Or are we just too late here, could the mucosa be destroyed so soon after death? With best regards, Mikael Niku -- //////////////////////////////////////////////////////////// Mikael Niku URL: www.helsinki.fi/~mniku/ University of Helsinki Dept. Basic Veterinary Sciences - Mit?k? mielt? olen l?nsimaisesta sivistyksest?? Minusta se olisi erinomainen ajatus! - Gandhi //////////////////////////////////////////////////////////// From JosefaNava <@t> texashealth.org Wed Mar 21 07:13:51 2007 From: JosefaNava <@t> texashealth.org (Nava, Josefa) Date: Wed Mar 21 07:14:01 2007 Subject: [Histonet] looking for ERCC1 antibody Message-ID: <2C515C1049EAF5459EFD8C9B929078A401DA1464@phdex03.txhealth.org> Hello Everyone, Can someone please tell me where I can order ERCC1 antibody that works best with Ventana BMK/XT. I appreciate your help. Josie The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. From rjbuesa <@t> yahoo.com Wed Mar 21 07:35:03 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 21 07:35:13 2007 Subject: [Histonet] Kurabo AS-200 In-Reply-To: <8F78639AC56F4143B267FE5F5A1B92C8F0ADED@guyton.phys.mcw.edu> Message-ID: <210099.99367.qm@web61213.mail.yahoo.com> The AS-200 system from Kurado, besides being still experimental from its medical application point of view, could be used to section "non-problematic", serial and "replaceable" blocks like those from large specimens from an autopsy, or for companies dedicated to producing histology slides for students (like the Carolina or the now defunct Turtox). This instrument, or any other similar that may be developed in the future, will never be able to substitute the histotech in the decision making: how deep to go into the block to get the adequate section?; which section to take and which to discard FOR EVER? Does the section just prepared reflect the whole area of the embeded tissue? Of the ones in the water bath, which are the best? Unless the "future" sectioning automaton if also equiped with a computer recognition system, it will not work as reliably as an experienced histotech. Even if that recognition system is incorporated, which are the standards to recognize? Each histology specimen has several unique characteristis: it is usually discrete, unique and irreplaceable and if lost is lost for EVER! Iwould not give to an automaton a specimen from a patient and hope that what ever it comes out of the machine is going to include and be representative of the lesion whose diagnostic is fundamental for that patient's care. This automaton "will not fly" in the medical practice with its present capabilities. Just my opinion (as always)! Ren? J. "Bobrowitz, Carol" wrote: Hi, Has anyone seen the Auto Slide Preparation System AS-200? It can replaces a Histotech! It can cut and stain. The company name is Kurabo Bio-Medical. http://www.bio.kurabo.co.jp/English/as200.html Bye, Bye, Carol Ann Bobrowitz MCW _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- We won't tell. Get more on shows you hate to love (and love to hate): Yahoo! TV's Guilty Pleasures list. From rjbuesa <@t> yahoo.com Wed Mar 21 07:39:35 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 21 07:39:40 2007 Subject: [Histonet] Problem with intestinal epithelium In-Reply-To: <46010D9E.7050305@helsinki.fi> Message-ID: <812969.76951.qm@web61220.mail.yahoo.com> 30 minutes from the moment the animal is slaughtered does not seem to be much.Stop all the washing and fix the tissue in NBF and wash after it is fixed. Ren? J. Mikael Niku wrote: Dear Histonetters, we are trying to make good-quality sections (paraffin and cryo) of bovine intestines. The problem is that the epithelium seems to be stripped off whatever we do. This seems to be a problem especially with slaughterhouse material, although the delay from death to sampling is less than 30 min. Currently we are cutting the intestine to pieces, washing with PBS buffer, and fixing immediately in buffered paraformaldehyde or freezing in isopentane / liquid nitrogen. Any ideas, anyone? Or are we just too late here, could the mucosa be destroyed so soon after death? With best regards, Mikael Niku -- //////////////////////////////////////////////////////////// Mikael Niku URL: www.helsinki.fi/~mniku/ University of Helsinki Dept. Basic Veterinary Sciences - Mit?k? mielt? olen l?nsimaisesta sivistyksest?? Minusta se olisi erinomainen ajatus! - Gandhi //////////////////////////////////////////////////////////// _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- TV dinner still cooling? Check out "Tonight's Picks" on Yahoo! TV. From b-frederick <@t> northwestern.edu Wed Mar 21 08:07:06 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Mar 21 08:07:20 2007 Subject: [Histonet] Octreotide Message-ID: <000001c76bb9$d3b254f0$d00f7ca5@lurie.northwestern.edu> Has anyone heard of the above mentioned antibody (Octreotide) let alone where it can be purchased? I have a researcher holding up his own project as his PI is not being very helpfule in giving him any info on it (like a paper from a journal). Thanks, Bernice Bernice Frederick Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 From greg_optimum <@t> yahoo.com Wed Mar 21 08:11:25 2007 From: greg_optimum <@t> yahoo.com (Greg Lyness) Date: Wed Mar 21 08:11:33 2007 Subject: [Histonet] Please remove me from the list Message-ID: <740077.67368.qm@web33611.mail.mud.yahoo.com> Please remove me from the Histonet newsletter list. Thank you, Greg Lyness --------------------------------- Food fight? Enjoy some healthy debate in the Yahoo! Answers Food & Drink Q&A. From HoustonR <@t> chi.osu.edu Wed Mar 21 08:25:31 2007 From: HoustonR <@t> chi.osu.edu (Houston, Ronald) Date: Wed Mar 21 08:25:46 2007 Subject: [Histonet] Octreotide Message-ID: <979FF5962E234F45B06CF0DB7C1AABB20D77D0C2@chi2k3ms01.columbuschildrens.net> I'm astonished that a researcher does not have the wherewithal or common sense to be able to perform his own search for information on a substance. Where would modern medicine be today if researchers of the past waited for someone else to provide them with information related to their area of interest? Point him/her in the direction of PubMed and Google and let them get on with their work. Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Columbus Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 houstonr@chi.osu.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Wednesday, March 21, 2007 9:07 AM To: histonet Subject: [Histonet] Octreotide Has anyone heard of the above mentioned antibody (Octreotide) let alone where it can be purchased? I have a researcher holding up his own project as his PI is not being very helpfule in giving him any info on it (like a paper from a journal). Thanks, Bernice Bernice Frederick Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: This e-mail message, from Children's Hospital, Columbus, Ohio, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes pursuant to Children's Hospital's confidentiality policies. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From vazquezr <@t> ohsu.edu Wed Mar 21 08:53:47 2007 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Wed Mar 21 08:54:15 2007 Subject: [Histonet] Kurabo AS-200 Message-ID: Patrick, Will we have to refer to them as Lord Robot or Hey you Bot? I am sorry couldn't help myself, it is too early for morning humor. I also agree that we cannot be replaced by automation. A machine can't mount it perfectly, rearrange if necessary (and to make that decision)and to recognize any problems as a human can see it. Just my 4 cents (inflation)!!! Robyn OHSU >>> "Patrick Laurie" 3/20/2007 3:42 PM >>> I completely agree with one of my coworkers, who said that he isn't worried until it can cut one of the difficult decals that he skillfully cut this morning. I, for one, am looking forward to servicing and maintaining our robot overlords. Patrick Laurie, HT (ASCP) Neurogenomics Laboratory Benaroya Research Institute 1201 9th Ave Seattle, Wa 98101 (206) 341-0681 Hi, Has anyone seen the Auto Slide Preparation System AS-200? It can replaces a Histotech! It can cut and stain. The company name is Kurabo Bio-Medical. http://www.bio.kurabo.co.jp/English/as200.html Bye, Bye, Carol Ann Bobrowitz MCW _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kappeler <@t> patho.unibe.ch Wed Mar 21 09:40:46 2007 From: kappeler <@t> patho.unibe.ch (Andi Kappeler) Date: Wed Mar 21 09:41:06 2007 Subject: [Histonet] CD138 (Clone B-B4) References: <37DC9F93CF7F864182D0463EF93D571B34AB35@ISLETON2.california.biogenex.com> Message-ID: <008e01c76bc6$e955f610$27955c82@patho.unibe.ch> Dear May I'm sorry, we did not try 5F7 - I had a hint from a colleague that MI15 would probably work well for our purposes, so we didn't spend more time and money to compare several possible clones (our finance people are grateful for that ...) Andi Kappeler ----- Original Message ----- From: "May Wei" To: "Andi Kappeler" Sent: Tuesday, March 20, 2007 7:02 PM Subject: RE: [Histonet] CD138 (Clone B-B4) Dear Andi, Did you try clone 5F7? If so, how is it compared with MI15? Thank you very much! May Wei BioGenex Laboratories -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andi Kappeler Sent: Monday, March 19, 2007 11:18 PM To: Luck, Greg D.; Histonet Subject: Re: [Histonet] CD138 (Clone B-B4) Hi Greg we were confronted with the same problem and have switched now to clone MI15, which gives us comparable results (on human FFPE tissue, mainly bone marrow). MI15 is from Dako, CatNo M7228. We use it at 1.8 ug Ig/ml, HIER in citrate, pH 6.0, pressure cooker. Hope this helps. Andi Kappeler Institute of Pathology, University of Bern, Switzerland ----- Original Message ----- From: "Luck, Greg D." To: Sent: Monday, March 19, 2007 10:16 PM Subject: [Histonet] CD138 (Clone B-B4) Hello all, I'm looking for a source the the Ab. and clone in my subject line. Our previous vendor (Serotec) informed us when we attempted to reorder that it was no longer available from them. Thanks, Greg Greg Luck, B.S., HT(ASCP) Anatomic Pathology Supervisor Deaconess Med Cntr 800 W. 5th Ave Spokane, WA 99204 Offc 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconessmc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victor <@t> pathology.washington.edu Wed Mar 21 09:41:48 2007 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Wed Mar 21 09:41:56 2007 Subject: [Histonet] Kurabo AS-200 In-Reply-To: References: Message-ID: <4601442C.5070709@pathology.washington.edu> The correct salutation is: Domo arigato, *Mr*. *Roboto* That is after he has cut boxes of serial sections for you for controls. Victor Robyn Vazquez wrote: > Patrick, > Will we have to refer to them as Lord Robot or Hey you Bot? I am sorry couldn't help myself, it is too early for morning humor. > I also agree that we cannot be replaced by automation. A machine can't mount it perfectly, rearrange if necessary (and to make that decision)and to recognize any problems as a human can see it. Just my 4 cents (inflation)!!! > > Robyn > OHSU > > >>>> "Patrick Laurie" 3/20/2007 3:42 PM >>> >>>> > > I completely agree with one of my coworkers, who said that he isn't > worried until it can cut one of the difficult decals that he skillfully > cut this morning. I, for one, am looking forward to servicing and > maintaining our robot overlords. > > > > > > Patrick Laurie, HT (ASCP) > Neurogenomics Laboratory > Benaroya Research Institute > 1201 9th Ave > Seattle, Wa 98101 > (206) 341-0681 > > Hi, > > > > > > > > Has anyone seen the Auto Slide Preparation System AS-200? > > > > It can replaces a Histotech! > > > > It can cut and stain. > > > > The company name is Kurabo Bio-Medical. > > > > http://www.bio.kurabo.co.jp/English/as200.html > > > > > > > > Bye, Bye, > > > > Carol Ann Bobrowitz > > > > MCW > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From LRaff <@t> lab.uropartners.com Wed Mar 21 09:54:06 2007 From: LRaff <@t> lab.uropartners.com (Lester Raff) Date: Wed Mar 21 09:54:15 2007 Subject: [Histonet] Kurabo AS-200 In-Reply-To: <4601442C.5070709@pathology.washington.edu> Message-ID: <5DA1CA5D0B98A84985B545A24423B822052DD5@UPLAB01.uplab.local> Last week it was Jimi Hendrix, this week it's Styx. What's next...REO Speedwagon? Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Wednesday, March 21, 2007 9:42 AM To: Robyn Vazquez Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Kurabo AS-200 The correct salutation is: Domo arigato, *Mr*. *Roboto* That is after he has cut boxes of serial sections for you for controls. Victor Robyn Vazquez wrote: > Patrick, > Will we have to refer to them as Lord Robot or Hey you Bot? I am sorry couldn't help myself, it is too early for morning humor. > I also agree that we cannot be replaced by automation. A machine can't mount it perfectly, rearrange if necessary (and to make that decision)and to recognize any problems as a human can see it. Just my 4 cents (inflation)!!! > > Robyn > OHSU > > >>>> "Patrick Laurie" 3/20/2007 3:42 PM >>> >>>> > > I completely agree with one of my coworkers, who said that he isn't > worried until it can cut one of the difficult decals that he skillfully > cut this morning. I, for one, am looking forward to servicing and > maintaining our robot overlords. > > > > > > Patrick Laurie, HT (ASCP) > Neurogenomics Laboratory > Benaroya Research Institute > 1201 9th Ave > Seattle, Wa 98101 > (206) 341-0681 > > Hi, > > > > > > > > Has anyone seen the Auto Slide Preparation System AS-200? > > > > It can replaces a Histotech! > > > > It can cut and stain. > > > > The company name is Kurabo Bio-Medical. > > > > http://www.bio.kurabo.co.jp/English/as200.html > > > > > > > > Bye, Bye, > > > > Carol Ann Bobrowitz > > > > MCW > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Mar 21 10:23:24 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 21 10:23:30 2007 Subject: [Histonet] Kurabo AS-200 In-Reply-To: <4601442C.5070709@pathology.washington.edu> Message-ID: <589597.82365.qm@web61214.mail.yahoo.com> More correct would be: "domo arigat? Roboto-san" Ren? J. Victor Tobias wrote: The correct salutation is: Domo arigato, *Mr*. *Roboto* That is after he has cut boxes of serial sections for you for controls. Victor Robyn Vazquez wrote: > Patrick, > Will we have to refer to them as Lord Robot or Hey you Bot? I am sorry couldn't help myself, it is too early for morning humor. > I also agree that we cannot be replaced by automation. A machine can't mount it perfectly, rearrange if necessary (and to make that decision)and to recognize any problems as a human can see it. Just my 4 cents (inflation)!!! > > Robyn > OHSU > > >>>> "Patrick Laurie" 3/20/2007 3:42 PM >>> >>>> > > I completely agree with one of my coworkers, who said that he isn't > worried until it can cut one of the difficult decals that he skillfully > cut this morning. I, for one, am looking forward to servicing and > maintaining our robot overlords. > > > > > > Patrick Laurie, HT (ASCP) > Neurogenomics Laboratory > Benaroya Research Institute > 1201 9th Ave > Seattle, Wa 98101 > (206) 341-0681 > > Hi, > > > > > > > > Has anyone seen the Auto Slide Preparation System AS-200? > > > > It can replaces a Histotech! > > > > It can cut and stain. > > > > The company name is Kurabo Bio-Medical. > > > > http://www.bio.kurabo.co.jp/English/as200.html > > > > > > > > Bye, Bye, > > > > Carol Ann Bobrowitz > > > > MCW > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Bored stiff? Loosen up... Download and play hundreds of games for free on Yahoo! Games. From akemiat3377 <@t> yahoo.com Wed Mar 21 10:40:13 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Wed Mar 21 10:40:19 2007 Subject: [Histonet] Kurabo AS-200 In-Reply-To: <4601442C.5070709@pathology.washington.edu> Message-ID: <854926.97981.qm@web31309.mail.mud.yahoo.com> I agree with you Victor. Being half Japanese and living and working in Japan, automation and computers are a way of life! This progression in automation was inevitable. The histology field is becoming totally automated and cutting tissues was the last craft we histo-techs performed. In the future there will be a instrument that will be fed a sample of tissue and a total analysis will be given. We dinosaurs are dying off. Better wake-up and smell the coffee and become comfortable with the computer age. DON'T KID YOURSELF. We are being replaced. Histology is becoming like the chemistry and hematology labs automated. What did Vinnie D. say at the NSH banquet? These kids are coming down the birth canal with a I Pod in their hand. There is a shortage of companies supplying the histology industry with quality control slides. Dako and Thermo have now discontinued selling quality control slides. The remaining companies are faced with the same problem as hospitals and private labs, NOT ENOUGH QUALIFIED HISTO-TECHS. This is a perfect answer for that niche. I know that there have been some disgruntled supervisors because of 2-3 month back orders of quality control slides. This may be acceptable with equipment purchases, but quality control slides? Just my 2 cents after working on both sides of the industry. Akemi Allison-Tacha --- Victor Tobias wrote: > The correct salutation is: > > Domo arigato, *Mr*. *Roboto* > > That is after he has cut boxes of serial sections > for you for controls. > > Victor > > Robyn Vazquez wrote: > > Patrick, > > Will we have to refer to them as Lord Robot or Hey > you Bot? I am sorry couldn't help myself, it is too > early for morning humor. > > I also agree that we cannot be replaced by > automation. A machine can't mount it perfectly, > rearrange if necessary (and to make that > decision)and to recognize any problems as a human > can see it. Just my 4 cents (inflation)!!! > > > > Robyn > > OHSU > > > > > >>>> "Patrick Laurie" > 3/20/2007 3:42 PM >>> > >>>> > > > > I completely agree with one of my coworkers, who > said that he isn't > > worried until it can cut one of the difficult > decals that he skillfully > > cut this morning. I, for one, am looking forward > to servicing and > > maintaining our robot overlords. > > > > > > > > > > > > Patrick Laurie, HT (ASCP) > > Neurogenomics Laboratory > > Benaroya Research Institute > > 1201 9th Ave > > Seattle, Wa 98101 > > (206) 341-0681 > > > > Hi, > > > > > > > > > > > > > > > > Has anyone seen the Auto Slide Preparation System > AS-200? > > > > > > > > It can replaces a Histotech! > > > > > > > > It can cut and stain. > > > > > > > > The company name is Kurabo Bio-Medical. > > > > > > > > http://www.bio.kurabo.co.jp/English/as200.html > > > > > > > > > > > > > > > > Bye, Bye, > > > > > > > > Carol Ann Bobrowitz > > > > > > > > MCW > > > > > > > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- > Victor Tobias > Clinical Applications Analyst > University of Washington Medical Center > Dept of Pathology Room BB220 > 1959 NE Pacific > Seattle, WA 98195 > victor@pathology.washington.edu > 206-598-2792 > 206-598-7659 Fax > ================================================= > Privileged, confidential or patient identifiable > information may be > contained in this message. This information is meant > only for the use > of the intended recipients. If you are not the > intended recipient, or > if the message has been addressed to you in error, > do not read, > disclose, reproduce, distribute, disseminate or > otherwise use this > transmission. Instead, please notify the sender by > reply e-mail, and > then destroy all copies of the message and any > attachments. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From AGrobe2555 <@t> aol.com Wed Mar 21 10:40:41 2007 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Wed Mar 21 10:41:05 2007 Subject: [Histonet] Re: Octreotide Message-ID: Having worked in research for the last 20 years or so, I have seen quite a bit of this...... People wish to be spoon-fed the information by others rather than making an effort to find it themselves. It is NOT the responsibility of the "PI" to provide all the answers for the researchers, just a general direction. It is then the responsibility of the researcher to to THEIR job (Research) and try to address the idea in an effective manner. Also, I have a difficulty understanding this persons difficulty in finding information especially with access to the internet. I really does help to have done your homework BEFORE you ask questions, so that you have a good idea whether the information received will be useful, and the answers should help clarify the issues rather than adding to the confusion..... Just my opinion, Albert ************************************** AOL now offers free email to everyone. Find out more about what's free from AOL at http://www.aol.com. From jessgrocki <@t> yahoo.com Wed Mar 21 10:42:44 2007 From: jessgrocki <@t> yahoo.com (Jessica Piche) Date: Wed Mar 21 10:42:52 2007 Subject: [Histonet] Paraffin Block Disposal Message-ID: <42911.85269.qm@web82011.mail.mud.yahoo.com> Hi, How is everyone disposing of Paraffin blocks? Are they considered Biohazardous waste or can they go in the regular trash? I cannot find anything on the CAP website and they are coming soon. Thanks! Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital From jessgrocki <@t> yahoo.com Wed Mar 21 10:44:17 2007 From: jessgrocki <@t> yahoo.com (Jessica Piche) Date: Wed Mar 21 10:44:27 2007 Subject: [Histonet] Sakura DRS-60 Stainer Message-ID: <20070321154417.73130.qmail@web82002.mail.mud.yahoo.com> Hello, We have a Sakura DRS-60 H&E stainer. For some reason the metal staining racks have suddenly become warped and the slides have started to fall out of the racks. Just wondering if anyone has any old racks hanging around that we could get our hands on. Anyone? Thanks, Jessica Piche-Grocki, HT(ASCP) From TMcNemar <@t> lmhealth.org Wed Mar 21 10:51:37 2007 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Wed Mar 21 10:51:33 2007 Subject: [Histonet] Sakura DRS-60 Stainer In-Reply-To: <20070321154417.73130.qmail@web82002.mail.mud.yahoo.com> Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F46D@lmhsmail.lmhealth.org> Jessica, It just so happens that I have three of these racks and 2 hangers. If you send me your mailing info, I'd be happy to send them to you. In the meantime, you can adjust them using the screws. It's a pain but can be done. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jessica Piche Sent: Wednesday, March 21, 2007 11:44 AM To: histonet Subject: [Histonet] Sakura DRS-60 Stainer Hello, We have a Sakura DRS-60 H&E stainer. For some reason the metal staining racks have suddenly become warped and the slides have started to fall out of the racks. Just wondering if anyone has any old racks hanging around that we could get our hands on. Anyone? Thanks, Jessica Piche-Grocki, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Wed Mar 21 11:04:01 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Mar 21 11:04:12 2007 Subject: AW: [Histonet] Kurabo AS-200 In-Reply-To: <4601442C.5070709@pathology.washington.edu> Message-ID: <001801c76bd2$8b4ea600$6412a8c0@dielangs.at> Why not *Mrs.* *Robota*? Gudrun The correct salutation is: Domo arigato, *Mr*. *Roboto* That is after he has cut boxes of serial sections for you for controls. Victor Robyn Vazquez wrote: > Patrick, > Will we have to refer to them as Lord Robot or Hey you Bot? I am sorry couldn't help myself, it is too early for morning humor. > I also agree that we cannot be replaced by automation. A machine can't mount it perfectly, rearrange if necessary (and to make that decision)and to recognize any problems as a human can see it. Just my 4 cents (inflation)!!! > > Robyn > OHSU > > >>>> "Patrick Laurie" 3/20/2007 3:42 PM >>> >>>> > > I completely agree with one of my coworkers, who said that he isn't > worried until it can cut one of the difficult decals that he skillfully > cut this morning. I, for one, am looking forward to servicing and > maintaining our robot overlords. > > > > > > Patrick Laurie, HT (ASCP) > Neurogenomics Laboratory > Benaroya Research Institute > 1201 9th Ave > Seattle, Wa 98101 > (206) 341-0681 > > Hi, > > > > > > > > Has anyone seen the Auto Slide Preparation System AS-200? > > > > It can replaces a Histotech! > > > > It can cut and stain. > > > > The company name is Kurabo Bio-Medical. > > > > http://www.bio.kurabo.co.jp/English/as200.html > > > > > > > > Bye, Bye, > > > > Carol Ann Bobrowitz > > > > MCW > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Wed Mar 21 11:09:54 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Mar 21 11:10:02 2007 Subject: [Histonet] background staining with ultra view from Ventana Message-ID: <001901c76bd3$5d5324f0$6412a8c0@dielangs.at> Hi to the Ventana users, Have any of you experienced increased background staining with the ultraview-DAB kit from Ventana in comparison to the iview-DAB. We have problems with this and I would like to know, if it is due to the reagens or to the machine (Benchmark XT). Thanks in advance Gudrun Lang From JMacDonald <@t> mtsac.edu Wed Mar 21 11:10:09 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Mar 21 11:10:20 2007 Subject: [Histonet] Problem with intestinal epithelium In-Reply-To: <46010D9E.7050305@helsinki.fi> Message-ID: We get bovine intestine from a local university that has an agriculture program. We provide them with a large container of formalin and ask that they open the intestine and place it immediately in the formalin. We have had great preservation of the epithelium. The piece of intestine is about 8-10 inches long. Jennifer Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu Mikael Niku Sent by: histonet-bounces@lists.utsouthwestern.edu 03/21/2007 03:49 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Problem with intestinal epithelium Dear Histonetters, we are trying to make good-quality sections (paraffin and cryo) of bovine intestines. The problem is that the epithelium seems to be stripped off whatever we do. This seems to be a problem especially with slaughterhouse material, although the delay from death to sampling is less than 30 min. Currently we are cutting the intestine to pieces, washing with PBS buffer, and fixing immediately in buffered paraformaldehyde or freezing in isopentane / liquid nitrogen. Any ideas, anyone? Or are we just too late here, could the mucosa be destroyed so soon after death? With best regards, Mikael Niku -- //////////////////////////////////////////////////////////// Mikael Niku URL: www.helsinki.fi/~mniku/ University of Helsinki Dept. Basic Veterinary Sciences - Mit?k? mielt? olen l?nsimaisesta sivistyksest?? Minusta se olisi erinomainen ajatus! - Gandhi //////////////////////////////////////////////////////////// _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Mar 21 11:12:54 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 21 11:13:04 2007 Subject: [Histonet] Paraffin Block Disposal In-Reply-To: <42911.85269.qm@web82011.mail.mud.yahoo.com> Message-ID: <363387.94890.qm@web61217.mail.yahoo.com> My SOP specified that old blocks (with more than 9 years in the files) were disposed off as used paraffin in red bags to be incinerated. That aspect of our SOP was acceptable to CAP inspectors. Ren? j. Jessica Piche wrote: Hi, How is everyone disposing of Paraffin blocks? Are they considered Biohazardous waste or can they go in the regular trash? I cannot find anything on the CAP website and they are coming soon. Thanks! Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- We won't tell. Get more on shows you hate to love (and love to hate): Yahoo! TV's Guilty Pleasures list. From Janet.Bonner <@t> FLHosp.org Wed Mar 21 11:13:47 2007 From: Janet.Bonner <@t> FLHosp.org (Bonner, Janet) Date: Wed Mar 21 11:17:11 2007 Subject: [Histonet] Kurabo AS-200 References: <8F78639AC56F4143B267FE5F5A1B92C8F0ADED@guyton.phys.mcw.edu> <08A0A863637F1349BBFD83A96B27A50A12000F@uwhis-xchng3.uwhis.hosp.wisc.edu> Message-ID: <5F31F38C96781A4FBE3196EBC22D478004A7BD@fhosxchmb006.ADVENTISTCORP.NET> We may not be interested but, unfortunately, replacing us with a machine eliminates a higher cost (our wages) over five years compared to the cost of the machine, benefits with possible workman's comp ( you know, the human aspect), and a unit that will work 24-7 -for starters. @:( ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Ingles Claire Sent: Tue 3/20/2007 6:25 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Kurabo AS-200 Why would we be interested in something that would replace us? This add makes me feel like some of the guys at the auto production plants when they started to become automated. I am NOT a robot. (and I don't work on unfeeling metal parts). Sorry, I also have a strong aversion to absolutes.(no, not the Vodka) Is it Friday yet? Claire Ingles UW Hospital and Clinics Madison WI ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Bobrowitz, Carol Sent: Tue 3/20/2007 3:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kurabo AS-200 Hi, Has anyone seen the Auto Slide Preparation System AS-200? It can replaces a Histotech! It can cut and stain. The company name is Kurabo Bio-Medical. http://www.bio.kurabo.co.jp/English/as200.html Bye, Bye, Carol Ann Bobrowitz MCW _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From ttruscot <@t> vetmed.wsu.edu Wed Mar 21 11:47:45 2007 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Wed Mar 21 11:47:58 2007 Subject: [Histonet] background staining with ultra view from Ventana In-Reply-To: <001901c76bd3$5d5324f0$6412a8c0@dielangs.at> References: <001901c76bd3$5d5324f0$6412a8c0@dielangs.at> Message-ID: <35CF12B690D8CA4E95375A36B4E7B44C06CECB@cvm36.vetmed.wsu.edu> Hi Gudrun, I've mostly run AEC on a older Benchmark, and then tried iView AlkPhos with mixed results varying with tissue type. I have then tried ultraView DAB and UltraView AlkPhos and immediately ran into background problems using my routine times and Ab concentrations. Debbie at Ventana tech support led me through some things to try. For the uView DAB, my best improvement came with less cell conditioning, shorter incubation times, my regular Ab concentrations, and Ventana Ab diluent alone in the ultrablock step. Happy Trials, Tom Truscott -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Wednesday, March 21, 2007 8:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] background staining with ultra view from Ventana Hi to the Ventana users, Have any of you experienced increased background staining with the ultraview-DAB kit from Ventana in comparison to the iview-DAB. We have problems with this and I would like to know, if it is due to the reagens or to the machine (Benchmark XT). Thanks in advance Gudrun Lang From ttruscot <@t> vetmed.wsu.edu Wed Mar 21 12:00:05 2007 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Wed Mar 21 12:00:12 2007 Subject: [Histonet] Kurabo AS-200 In-Reply-To: <5F31F38C96781A4FBE3196EBC22D478004A7BD@fhosxchmb006.ADVENTISTCORP.NET> References: <8F78639AC56F4143B267FE5F5A1B92C8F0ADED@guyton.phys.mcw.edu><08A0A863637F1349BBFD83A96B27A50A12000F@uwhis-xchng3.uwhis.hosp.wisc.edu> <5F31F38C96781A4FBE3196EBC22D478004A7BD@fhosxchmb006.ADVENTISTCORP.NET> Message-ID: <35CF12B690D8CA4E95375A36B4E7B44C06CECC@cvm36.vetmed.wsu.edu> Some automated machines create as much work as they save, and need a well trained person to operate, troubleshoot, and maintain; as well as plan and maintain it's workload. So just adjust and don't look for any big job losses or big money savings yet. Tom Truscott -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonner, Janet Sent: Wednesday, March 21, 2007 8:14 AM To: Ingles Claire; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Kurabo AS-200 We may not be interested but, unfortunately, replacing us with a machine eliminates a higher cost (our wages) over five years compared to the cost of the machine, benefits with possible workman's comp ( you know, the human aspect), and a unit that will work 24-7 -for starters. @:( ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Ingles Claire Sent: Tue 3/20/2007 6:25 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Kurabo AS-200 Why would we be interested in something that would replace us? This add makes me feel like some of the guys at the auto production plants when they started to become automated. I am NOT a robot. (and I don't work on unfeeling metal parts). Sorry, I also have a strong aversion to absolutes.(no, not the Vodka) Is it Friday yet? Claire Ingles UW Hospital and Clinics Madison WI ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Bobrowitz, Carol Sent: Tue 3/20/2007 3:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kurabo AS-200 Hi, Has anyone seen the Auto Slide Preparation System AS-200? It can replaces a Histotech! It can cut and stain. The company name is Kurabo Bio-Medical. http://www.bio.kurabo.co.jp/English/as200.html Bye, Bye, Carol Ann Bobrowitz MCW _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Wed Mar 21 12:04:34 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Wed Mar 21 12:04:47 2007 Subject: AW: [Histonet] Kurabo AS-200 In-Reply-To: <001801c76bd2$8b4ea600$6412a8c0@dielangs.at> Message-ID: <613485.29829.qm@web31310.mail.mud.yahoo.com> Gundrun, gomenasei, but I prefer *Ms*. Always have, always will!! Women rock without being an appendage!! We just need to value ourselves. Akemi Allison-Tacha --- Gudrun Lang wrote: > Why not *Mrs.* *Robota*? > > Gudrun > > > > The correct salutation is: > > Domo arigato, *Mr*. *Roboto* > > That is after he has cut boxes of serial sections > for you for controls. > > Victor > > Robyn Vazquez wrote: > > Patrick, > > Will we have to refer to them as Lord Robot or Hey > you Bot? I am sorry > couldn't help myself, it is too early for morning > humor. > > I also agree that we cannot be replaced by > automation. A machine can't > mount it perfectly, rearrange if necessary (and to > make that decision)and to > recognize any problems as a human can see it. Just > my 4 cents > (inflation)!!! > > > > Robyn > > OHSU > > > > > >>>> "Patrick Laurie" > 3/20/2007 3:42 PM >>> > >>>> > > > > I completely agree with one of my coworkers, who > said that he isn't > > worried until it can cut one of the difficult > decals that he skillfully > > cut this morning. I, for one, am looking forward > to servicing and > > maintaining our robot overlords. > > > > > > > > > > > > Patrick Laurie, HT (ASCP) > > Neurogenomics Laboratory > > Benaroya Research Institute > > 1201 9th Ave > > Seattle, Wa 98101 > > (206) 341-0681 > > > > Hi, > > > > > > > > > > > > > > > > Has anyone seen the Auto Slide Preparation System > AS-200? > > > > > > > > It can replaces a Histotech! > > > > > > > > It can cut and stain. > > > > > > > > The company name is Kurabo Bio-Medical. > > > > > > > > http://www.bio.kurabo.co.jp/English/as200.html > > > > > > > > > > > > > > > > Bye, Bye, > > > > > > > > Carol Ann Bobrowitz > > > > > > > > MCW > > > > > > > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- > Victor Tobias > Clinical Applications Analyst > University of Washington Medical Center > Dept of Pathology Room BB220 > 1959 NE Pacific > Seattle, WA 98195 > victor@pathology.washington.edu > 206-598-2792 > 206-598-7659 Fax > ================================================= > Privileged, confidential or patient identifiable > information may be > contained in this message. This information is meant > only for the use > of the intended recipients. If you are not the > intended recipient, or > if the message has been addressed to you in error, > do not read, > disclose, reproduce, distribute, disseminate or > otherwise use this > transmission. Instead, please notify the sender by > reply e-mail, and > then destroy all copies of the message and any > attachments. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From akemiat3377 <@t> yahoo.com Wed Mar 21 13:03:33 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Wed Mar 21 13:03:41 2007 Subject: AW: [Histonet] Kurabo AS-200 In-Reply-To: <613485.29829.qm@web31310.mail.mud.yahoo.com> Message-ID: <675955.12034.qm@web31302.mail.mud.yahoo.com> OK guys, uncle! I have been seriously thinking of taking the TACHA off my name after the divorce!! I have always had my own insecurity issues to deal with. After all, my mother was a subservient old world Japanese women who gave up everything for her man. Looks may be deceiving, we all ware masks! Akemi Hungerford --- Akemi Allison-Tacha wrote: > Gundrun, gomenasei, but I prefer *Ms*. Always have, > always will!! Women rock without being an > appendage!! > We just need to value ourselves. > > Akemi Allison-Tacha > > --- Gudrun Lang wrote: > > > Why not *Mrs.* *Robota*? > > > > Gudrun > > > > > > > > The correct salutation is: > > > > Domo arigato, *Mr*. *Roboto* > > > > That is after he has cut boxes of serial sections > > for you for controls. > > > > Victor > > > > Robyn Vazquez wrote: > > > Patrick, > > > Will we have to refer to them as Lord Robot or > Hey > > you Bot? I am sorry > > couldn't help myself, it is too early for morning > > humor. > > > I also agree that we cannot be replaced by > > automation. A machine can't > > mount it perfectly, rearrange if necessary (and to > > make that decision)and to > > recognize any problems as a human can see it. > Just > > my 4 cents > > (inflation)!!! > > > > > > Robyn > > > OHSU > > > > > > > > >>>> "Patrick Laurie" > > > 3/20/2007 3:42 PM >>> > > >>>> > > > > > > I completely agree with one of my coworkers, who > > said that he isn't > > > worried until it can cut one of the difficult > > decals that he skillfully > > > cut this morning. I, for one, am looking > forward > > to servicing and > > > maintaining our robot overlords. > > > > > > > > > > > > > > > > > > Patrick Laurie, HT (ASCP) > > > Neurogenomics Laboratory > > > Benaroya Research Institute > > > 1201 9th Ave > > > Seattle, Wa 98101 > > > (206) 341-0681 > > > > > > Hi, > > > > > > > > > > > > > > > > > > > > > > > > Has anyone seen the Auto Slide Preparation > System > > AS-200? > > > > > > > > > > > > It can replaces a Histotech! > > > > > > > > > > > > It can cut and stain. > > > > > > > > > > > > The company name is Kurabo Bio-Medical. > > > > > > > > > > > > http://www.bio.kurabo.co.jp/English/as200.html > > > > > > > > > > > > > > > > > > > > > > > > Bye, Bye, > > > > > > > > > > > > Carol Ann Bobrowitz > > > > > > > > > > > > MCW > > > > > > > > > > > > > > > > > > > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > -- > > Victor Tobias > > Clinical Applications Analyst > > University of Washington Medical Center > > Dept of Pathology Room BB220 > > 1959 NE Pacific > > Seattle, WA 98195 > > victor@pathology.washington.edu > > 206-598-2792 > > 206-598-7659 Fax > > ================================================= > > Privileged, confidential or patient identifiable > > information may be > > contained in this message. This information is > meant > > only for the use > > of the intended recipients. If you are not the > > intended recipient, or > > if the message has been addressed to you in error, > > do not read, > > disclose, reproduce, distribute, disseminate or > > otherwise use this > > transmission. Instead, please notify the sender by > > reply e-mail, and > > then destroy all copies of the message and any > > attachments. > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From JosefaNava <@t> texashealth.org Wed Mar 21 13:18:13 2007 From: JosefaNava <@t> texashealth.org (Nava, Josefa) Date: Wed Mar 21 13:18:27 2007 Subject: [Histonet] Looking for P57 , mammoglobin antibodies Message-ID: <2C515C1049EAF5459EFD8C9B929078A401DA1469@phdex03.txhealth.org> Hello, Please tell me where I can get P57, and Mammoglobin (MGB1) antibodies that I can run on our Ventana BMK/XT machines. Thank you. Josie The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. From gu.lang <@t> gmx.at Wed Mar 21 13:22:59 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Mar 21 13:23:11 2007 Subject: AW: [Histonet] background staining with ultra view from Ventana In-Reply-To: <35CF12B690D8CA4E95375A36B4E7B44C06CECB@cvm36.vetmed.wsu.edu> Message-ID: <001301c76be5$f4d82fc0$6412a8c0@dielangs.at> Hi Tom, most of my protocols are with CC1 mild (30min) and 24 min Antibody-incubation. Do you think this durations should be shortened? Wouldn't it need an increase of the antibody-concentration on the other hand? Have you seen a change in the background from lot to lot? Because we have used the u-view for about six months now, and never had this big problems. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: Truscott, Tom [mailto:ttruscot@vetmed.wsu.edu] Gesendet: Mittwoch, 21. M?rz 2007 17:48 An: gu.lang@gmx.at; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] background staining with ultra view from Ventana Hi Gudrun, I've mostly run AEC on a older Benchmark, and then tried iView AlkPhos with mixed results varying with tissue type. I have then tried ultraView DAB and UltraView AlkPhos and immediately ran into background problems using my routine times and Ab concentrations. Debbie at Ventana tech support led me through some things to try. For the uView DAB, my best improvement came with less cell conditioning, shorter incubation times, my regular Ab concentrations, and Ventana Ab diluent alone in the ultrablock step. Happy Trials, Tom Truscott -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Wednesday, March 21, 2007 8:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] background staining with ultra view from Ventana Hi to the Ventana users, Have any of you experienced increased background staining with the ultraview-DAB kit from Ventana in comparison to the iview-DAB. We have problems with this and I would like to know, if it is due to the reagens or to the machine (Benchmark XT). Thanks in advance Gudrun Lang From DennisH <@t> cookchildrens.org Wed Mar 21 14:17:09 2007 From: DennisH <@t> cookchildrens.org (Dennis Hahn) Date: Wed Mar 21 14:17:31 2007 Subject: [Histonet] Kurabo AS-200 Message-ID: I would like to try one of these. If it could go through my email and pick out the important ones from all of the garbage, then I'll move it over to the embedding station. With only large, simple tissues first, of course. Then he, or she, can graduate up to embedding biopsies. I'll even create an orientation and training packet just in case CAP wants to see my employee training files....and you all know that would be added to the checklist if we actually brought in robots! Dennis Dennis Hahn, HT (ASCP) Histology Lab Supervisor Cook Children's Medical Center 801 7th Avenue Ft Worth, Tx 76104-2796 682-885-6168 dennish@cookchildrens.org >>> "Bonner, Janet" 3/21/2007 11:13 AM >>> We may not be interested but, unfortunately, replacing us with a machine eliminates a higher cost (our wages) over five years compared to the cost of the machine, benefits with possible workman's comp ( you know, the human aspect), and a unit that will work 24-7 -for starters. @:( ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Ingles Claire Sent: Tue 3/20/2007 6:25 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Kurabo AS-200 Why would we be interested in something that would replace us? This add makes me feel like some of the guys at the auto production plants when they started to become automated. I am NOT a robot. (and I don't work on unfeeling metal parts). Sorry, I also have a strong aversion to absolutes.(no, not the Vodka) Is it Friday yet? Claire Ingles UW Hospital and Clinics Madison WI ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Bobrowitz, Carol Sent: Tue 3/20/2007 3:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kurabo AS-200 Hi, Has anyone seen the Auto Slide Preparation System AS-200? It can replaces a Histotech! It can cut and stain. The company name is Kurabo Bio-Medical. http://www.bio.kurabo.co.jp/English/as200.html Bye, Bye, Carol Ann Bobrowitz MCW _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------------------------------------ Cook Children's Health Care System This e-mail, facsimile, or letter and any files or attachments transmitted may contain information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Cook Children's Health Care System immediately at (682)885-4000 or via e-mail at compliance@cookchildrens.org. Cook Children's Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. ----------------------------------------------------------------------------------------------------------- From RCazares <@t> schosp.org Wed Mar 21 14:55:30 2007 From: RCazares <@t> schosp.org (Cazares, Ruth) Date: Wed Mar 21 14:55:53 2007 Subject: [Histonet] Re: Kurabo AS 200 Message-ID: <913FAC2B773C19488E26AE6572180FA509BF013F@exch01.schosp.org> Well I have to admit, curiosity got the best of me and I went on the website to checkout my (and every histo tech's) competition. It does seem impressive, and it can crank out 200 slides in 2 hours (one run equals 2 hours), BUT those are serial sections from one block. If you want different blocks cut it can handle up to; WAIT FOR IT...... HERE IT COMES........ 20, YES! Twenty blocks every run or 2 hours. So, if your lab averages 200 blocks a day it would take 10 hours to get them cut and stained. I don't think that would work in my lab, but that's just my opinion. I have enclosed the website in case anyone else is curious. http://www.bio.kurabo.co.jp/English/as200.html Ruth Cazares, Histology Supervisor (and histo tech, for the time being) Department of Pathology Swedish Covenant Hospital Chicago, IL 60625 *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. From pmarcum <@t> vet.upenn.edu Wed Mar 21 15:09:41 2007 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Wed Mar 21 15:09:49 2007 Subject: [Histonet] Program for NSH in Denver Message-ID: <6.2.5.6.2.20070321160608.01c8e370@vet.upenn.edu> Has anyone gotten an actual program from NSH for the meeting? It really does not matter to me in one way as it is too late to apply to go with the University at this point. I don't know about other facilities requirements however we have to justify why we are going and show the classes before it is cleared. That must be done at least by the end of February for my area. It has gotten later every year!! Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From Janet.Bonner <@t> flhosp.org Wed Mar 21 15:11:27 2007 From: Janet.Bonner <@t> flhosp.org (Bonner, Janet) Date: Wed Mar 21 15:12:30 2007 Subject: [Histonet] Re: Kurabo AS 200 References: <913FAC2B773C19488E26AE6572180FA509BF013F@exch01.schosp.org> Message-ID: <5F31F38C96781A4FBE3196EBC22D478004A7C1@fhosxchmb006.ADVENTISTCORP.NET> Well, we have 1000 blocks per day! I feel much better right about now!!!! ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Cazares, Ruth Sent: Wed 3/21/2007 3:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Kurabo AS 200 Well I have to admit, curiosity got the best of me and I went on the website to checkout my (and every histo tech's) competition. It does seem impressive, and it can crank out 200 slides in 2 hours (one run equals 2 hours), BUT those are serial sections from one block. If you want different blocks cut it can handle up to; WAIT FOR IT...... HERE IT COMES........ 20, YES! Twenty blocks every run or 2 hours. So, if your lab averages 200 blocks a day it would take 10 hours to get them cut and stained. I don't think that would work in my lab, but that's just my opinion. I have enclosed the website in case anyone else is curious. http://www.bio.kurabo.co.jp/English/as200.html Ruth Cazares, Histology Supervisor (and histo tech, for the time being) Department of Pathology Swedish Covenant Hospital Chicago, IL 60625 *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From HoustonR <@t> chi.osu.edu Wed Mar 21 15:14:00 2007 From: HoustonR <@t> chi.osu.edu (Houston, Ronald) Date: Wed Mar 21 15:14:41 2007 Subject: [Histonet] Re: Kurabo AS 200 Message-ID: <979FF5962E234F45B06CF0DB7C1AABB20D77D0D0@chi2k3ms01.columbuschildrens.net> Makes more sense why someone would want to purchase this if you go to the Japanese translation site Ronnie Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Columbus Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 houstonr@chi.osu.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cazares, Ruth Sent: Wednesday, March 21, 2007 3:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Kurabo AS 200 Well I have to admit, curiosity got the best of me and I went on the website to checkout my (and every histo tech's) competition. It does seem impressive, and it can crank out 200 slides in 2 hours (one run equals 2 hours), BUT those are serial sections from one block. If you want different blocks cut it can handle up to; WAIT FOR IT...... HERE IT COMES........ 20, YES! Twenty blocks every run or 2 hours. So, if your lab averages 200 blocks a day it would take 10 hours to get them cut and stained. I don't think that would work in my lab, but that's just my opinion. I have enclosed the website in case anyone else is curious. http://www.bio.kurabo.co.jp/English/as200.html Ruth Cazares, Histology Supervisor (and histo tech, for the time being) Department of Pathology Swedish Covenant Hospital Chicago, IL 60625 *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: This e-mail message, from Children's Hospital, Columbus, Ohio, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes pursuant to Children's Hospital's confidentiality policies. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From LGaliotto <@t> nch.org Wed Mar 21 15:21:55 2007 From: LGaliotto <@t> nch.org (Galiotto, Laura) Date: Wed Mar 21 15:22:03 2007 Subject: [Histonet] delivery time of last tray of H&E Message-ID: <270614B321ACB44D8C1D91F4F921FDC304B163C5@NCH01EX02.nch.org> Hello Fellow Histo Tech's, Will anyone be willing to share the delivery time of the last tray of H&E for the days workload to the pathologist reading them? What is your cutoff time for specimen acceptance? What is your annual case workload? I am trying to improve the TAT from specimen gross to the H&E delivered to the pathologist. Unless we change the cutoff time I can not see how I can do this? Any suggestions? We process 22,000 cases a year an average of 280 blks a day consisting of types of tissue. Please Help Laura ******************* PLEASE NOTE ******************* This E-Mail/telefax message and any documents accompanying this transmission may contain information that is privileged, confidential, and/or exempt from disclosure under applicable law and is intended solely for the addressee(s) named above. If you are not the intended addressee/recipient, you are hereby notified that any use of, disclosure, copying, distribution, or reliance on the contents of this E-Mail/telefax information is strictly prohibited and may result in legal action against you. Please reply to the sender advising of the error in transmission and immediately delete/destroy the message and any accompanying documents. Thank you. From doug <@t> ppspath.com Wed Mar 21 16:37:33 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Wed Mar 21 15:36:03 2007 Subject: [Histonet] delivery time of last tray of H&E In-Reply-To: <270614B321ACB44D8C1D91F4F921FDC304B163C5@NCH01EX02.nch.org> Message-ID: Laura, There are many other variables that need to be known like how many processors you have, how many techs you have, what are their schedules, when is the first processor loaded and when does it come off. Please elaborate. Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Galiotto, Laura Sent: Wednesday, March 21, 2007 3:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] delivery time of last tray of H&E Hello Fellow Histo Tech's, Will anyone be willing to share the delivery time of the last tray of H&E for the days workload to the pathologist reading them? What is your cutoff time for specimen acceptance? What is your annual case workload? I am trying to improve the TAT from specimen gross to the H&E delivered to the pathologist. Unless we change the cutoff time I can not see how I can do this? Any suggestions? We process 22,000 cases a year an average of 280 blks a day consisting of types of tissue. Please Help Laura ******************* PLEASE NOTE ******************* This E-Mail/telefax message and any documents accompanying this transmission may contain information that is privileged, confidential, and/or exempt from disclosure under applicable law and is intended solely for the addressee(s) named above. If you are not the intended addressee/recipient, you are hereby notified that any use of, disclosure, copying, distribution, or reliance on the contents of this E-Mail/telefax information is strictly prohibited and may result in legal action against you. Please reply to the sender advising of the error in transmission and immediately delete/destroy the message and any accompanying documents. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Wed Mar 21 16:46:38 2007 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed Mar 21 16:46:42 2007 Subject: [Histonet] Program for NSH in Denver References: <6.2.5.6.2.20070321160608.01c8e370@vet.upenn.edu> Message-ID: I went to their website and the actual workshops are not yet listed. I would email them directly, but I don't remember ever getting a program this early. Jeanine Bartlett CDC/Atlanta -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Pamela Marcum Sent: Wed 3/21/2007 4:09 PM To: histonet@lists.utsouthwestern.edu Cc: Subject: [Histonet] Program for NSH in Denver Has anyone gotten an actual program from NSH for the meeting? It really does not matter to me in one way as it is too late to apply to go with the University at this point. I don't know about other facilities requirements however we have to justify why we are going and show the classes before it is cleared. That must be done at least by the end of February for my area. It has gotten later every year!! Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nsnwl <@t> neuro.hfh.edu Wed Mar 21 17:00:09 2007 From: nsnwl <@t> neuro.hfh.edu (Nancy Lemke) Date: Wed Mar 21 17:00:30 2007 Subject: [Histonet] Program for NSH in Denver Message-ID: The educational sessions are listed in detail on the website. Nancy Lemke Research Coordinator Hermelin Brain Tumor Center Henry Ford Hospital Detroit -----Original message----- From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" jqb7@cdc.gov Date: Wed, 21 Mar 2007 17:48:10 -0400 To: "Pamela Marcum" pmarcum@vet.upenn.edu Subject: RE: [Histonet] Program for NSH in Denver > I went to their website and the actual workshops are not > yet listed. I would email them directly, but I don't rem> ember ever getting a program this early. Jeanine Bart> lett CDC/Atlanta -----Original Message----- From:> histonet-bounces@lists.utsouthwestern.edu on behalf of P> amela Marcum Sent: Wed 3/21/2007 4:09 PM To: histon> et@lists.utsouthwestern.edu Cc: Subject: [Histonet]> Program for NSH in Denver Has anyone gotte> n an actual program from NSH for the meeting? It reall> y does not matter to me in one way as it is too late to a> pply to go with the University at this point. I don't > know about other facilities requirements however we hav> e to justify why we are going and show the classes befo> re it is cleared. That must be done at least by the en> d of February for my area. It has gotten later every yea> r!! Best Regards, Pamela A Marcum Manag> er, Histology Special Procedures University of Pennsylv> ania School of Veterinary Medicine R.S. Reynolds Jr. > CORL New Bolton Center 382 West Street Road Kennet> t Square, PA 19348 Phone - 610-925-6278 Fax - > 610-925-8120 E-mail - pmarcum@vet.upenn.edu ___> ____________________________________________ Histonet m> ailing list Histonet@lists.utsouthwestern.edu http://> lists.utsouthwestern.edu/mailman/listinfo/histonet > ============================================================================== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================== From jqb7 <@t> cdc.gov Wed Mar 21 17:05:59 2007 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed Mar 21 17:06:04 2007 Subject: [Histonet] Program for NSH in Denver References: Message-ID: Thanks. I just looked under schedule and all the workshops were simply listed as "workshops". Jeanine Bartlett CDC/Atlanta -----Original Message----- From: Nancy Lemke [mailto:nsnwl@neuro.hfh.edu] Sent: Wed 3/21/2007 6:00 PM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Pamela Marcum; histonet@lists.utsouthwestern.edu Cc: Subject: RE: [Histonet] Program for NSH in Denver The educational sessions are listed in detail on the website. Nancy Lemke Research Coordinator Hermelin Brain Tumor Center Henry Ford Hospital Detroit -----Original message----- From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" jqb7@cdc.gov Date: Wed, 21 Mar 2007 17:48:10 -0400 To: "Pamela Marcum" pmarcum@vet.upenn.edu Subject: RE: [Histonet] Program for NSH in Denver > I went to their website and the actual workshops are not > yet listed. I would email them directly, but I don't rem> ember ever getting a program this early. Jeanine Bart> lett CDC/Atlanta -----Original Message----- From:> histonet-bounces@lists.utsouthwestern.edu on behalf of P> amela Marcum Sent: Wed 3/21/2007 4:09 PM To: histon> et@lists.utsouthwestern.edu Cc: Subject: [Histonet]> Program for NSH in Denver Has anyone gotte> n an actual program from NSH for the meeting? It reall> y does not matter to me in one way as it is too late to a> pply to go with the University at this point. I don't > know about other facilities requirements however we hav> e to justify why we are going and show the classes befo> re it is cleared. That must be done at least by the en> d of February for my area. It has gotten later every yea> r!! Best Regards, Pamela A Marcum Manag> er, Histology Special Procedures University of Pennsylv> ania School of Veterinary Medicine R.S. Reynolds Jr. > CORL New Bolton Center 382 West Street Road Kennet> t Square, PA 19348 Phone - 610-925-6278 Fax - > 610-925-8120 E-mail - pmarcum@vet.upenn.edu ___> ____________________________________________ Histonet m> ailing list Histonet@lists.utsouthwestern.edu http:// > lists.utsouthwestern.edu/mailman/listinfo/histonet > ============================================================================== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================== From jnocito <@t> satx.rr.com Wed Mar 21 17:20:34 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Mar 21 17:20:41 2007 Subject: [Histonet] background staining with ultra view from Ventana References: <001901c76bd3$5d5324f0$6412a8c0@dielangs.at> Message-ID: <004101c76c07$2633ac40$11614542@yourxhtr8hvc4p> oh no, not again!! Last time I answered a question regarding this, I almost lost my job and was told to get off the Histonet. Nah, not worth it. I'll keep my mouth shut this time. See, you can teach an ole dog new tricks. JTT ----- Original Message ----- From: "Gudrun Lang" To: Sent: Wednesday, March 21, 2007 11:09 AM Subject: [Histonet] background staining with ultra view from Ventana > Hi to the Ventana users, > > Have any of you experienced increased background staining with the > ultraview-DAB kit from Ventana in comparison to the iview-DAB. We have > problems with this and I would like to know, if it is due to the reagens > or > to the machine (Benchmark XT). > > > > Thanks in advance > > Gudrun Lang > > -------------------------------------------------------------------------------- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jnocito <@t> satx.rr.com Wed Mar 21 17:24:16 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Mar 21 17:26:16 2007 Subject: [Histonet] Kurabo AS-200 References: <001801c76bd2$8b4ea600$6412a8c0@dielangs.at> Message-ID: <008f01c76c07$e60b7570$11614542@yourxhtr8hvc4p> yeah but, can it tell jokes? JTT ----- Original Message ----- From: "Gudrun Lang" To: Sent: Wednesday, March 21, 2007 11:04 AM Subject: AW: [Histonet] Kurabo AS-200 > Why not *Mrs.* *Robota*? > > Gudrun > > > > The correct salutation is: > > Domo arigato, *Mr*. *Roboto* > > That is after he has cut boxes of serial sections for you for controls. > > Victor > > Robyn Vazquez wrote: >> Patrick, >> Will we have to refer to them as Lord Robot or Hey you Bot? I am sorry > couldn't help myself, it is too early for morning humor. >> I also agree that we cannot be replaced by automation. A machine can't > mount it perfectly, rearrange if necessary (and to make that decision)and > to > recognize any problems as a human can see it. Just my 4 cents > (inflation)!!! >> >> Robyn >> OHSU >> >> >>>>> "Patrick Laurie" 3/20/2007 3:42 PM >>> >>>>> >> >> I completely agree with one of my coworkers, who said that he isn't >> worried until it can cut one of the difficult decals that he skillfully >> cut this morning. I, for one, am looking forward to servicing and >> maintaining our robot overlords. >> >> >> >> >> >> Patrick Laurie, HT (ASCP) >> Neurogenomics Laboratory >> Benaroya Research Institute >> 1201 9th Ave >> Seattle, Wa 98101 >> (206) 341-0681 >> >> Hi, >> >> >> >> >> >> >> >> Has anyone seen the Auto Slide Preparation System AS-200? >> >> >> >> It can replaces a Histotech! >> >> >> >> It can cut and stain. >> >> >> >> The company name is Kurabo Bio-Medical. >> >> >> >> http://www.bio.kurabo.co.jp/English/as200.html >> >> >> >> >> >> >> >> Bye, Bye, >> >> >> >> Carol Ann Bobrowitz >> >> >> >> MCW >> >> >> >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > -- > Victor Tobias > Clinical Applications Analyst > University of Washington Medical Center > Dept of Pathology Room BB220 > 1959 NE Pacific > Seattle, WA 98195 > victor@pathology.washington.edu > 206-598-2792 > 206-598-7659 Fax > ================================================= > Privileged, confidential or patient identifiable information may be > contained in this message. This information is meant only for the use > of the intended recipients. If you are not the intended recipient, or > if the message has been addressed to you in error, do not read, > disclose, reproduce, distribute, disseminate or otherwise use this > transmission. Instead, please notify the sender by reply e-mail, and > then destroy all copies of the message and any attachments. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Wed Mar 21 17:27:05 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Mar 21 17:27:14 2007 Subject: [Histonet] Re: Kurabo AS 200 References: <913FAC2B773C19488E26AE6572180FA509BF013F@exch01.schosp.org> Message-ID: <00ad01c76c08$0ef78870$11614542@yourxhtr8hvc4p> it's still faster than some techs I know. JTT ----- Original Message ----- From: "Cazares, Ruth" To: Sent: Wednesday, March 21, 2007 2:55 PM Subject: [Histonet] Re: Kurabo AS 200 Well I have to admit, curiosity got the best of me and I went on the website to checkout my (and every histo tech's) competition. It does seem impressive, and it can crank out 200 slides in 2 hours (one run equals 2 hours), BUT those are serial sections from one block. If you want different blocks cut it can handle up to; WAIT FOR IT...... HERE IT COMES........ 20, YES! Twenty blocks every run or 2 hours. So, if your lab averages 200 blocks a day it would take 10 hours to get them cut and stained. I don't think that would work in my lab, but that's just my opinion. I have enclosed the website in case anyone else is curious. http://www.bio.kurabo.co.jp/English/as200.html Ruth Cazares, Histology Supervisor (and histo tech, for the time being) Department of Pathology Swedish Covenant Hospital Chicago, IL 60625 *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Luis.Chiriboga <@t> med.nyu.edu Thu Mar 22 08:34:27 2007 From: Luis.Chiriboga <@t> med.nyu.edu (Luis Chiriboga) Date: Thu Mar 22 07:28:00 2007 Subject: [Histonet] NYSHS Spring Meeting - Canandaigua NY - May 4th - 5th Message-ID: The New York State Histotechnological Society is pleased to announce it's Annual Symposium Convention May 4-5th, 2007 at The Inn on the Lake in Canandaigua, NY. The program and registration information are available on our website at: nyhisto.org/meetings We hope to see you there! ____________________________________ From b-frederick <@t> northwestern.edu Thu Mar 22 07:58:48 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Mar 22 07:59:09 2007 Subject: [Histonet] Kurabo AS-200 In-Reply-To: <008f01c76c07$e60b7570$11614542@yourxhtr8hvc4p> Message-ID: <000c01c76c81$d9383930$d00f7ca5@lurie.northwestern.edu> To All: I can see this: Receive block from outside institution (anywhere in world) deidentify block by giving unique ID Save "trims" for DNA Etch slides so that each slide is an aliquot of the block with its own unique ID Cut 12 (or more) slides and place on slide so they can be scanned into ACISII Cut sections for RNA And may I mention ALL under sterile conditions which is clean blade,fresh water, wipe down microtome w/alcohol and forceps etc. after every block? Then, after H&E is QC'ed and area of interest indicated, a TMA must be mapped and made. Not to mention IHC and extrapolation of data from Path reports!!! A robot- FAT CHANCE Bernice -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, March 21, 2007 4:24 PM To: gu.lang@gmx.at; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Kurabo AS-200 yeah but, can it tell jokes? JTT ----- Original Message ----- From: "Gudrun Lang" To: Sent: Wednesday, March 21, 2007 11:04 AM Subject: AW: [Histonet] Kurabo AS-200 > Why not *Mrs.* *Robota*? > > Gudrun > > > > The correct salutation is: > > Domo arigato, *Mr*. *Roboto* > > That is after he has cut boxes of serial sections for you for controls. > > Victor > > Robyn Vazquez wrote: >> Patrick, >> Will we have to refer to them as Lord Robot or Hey you Bot? I am sorry > couldn't help myself, it is too early for morning humor. >> I also agree that we cannot be replaced by automation. A machine can't > mount it perfectly, rearrange if necessary (and to make that decision)and > to > recognize any problems as a human can see it. Just my 4 cents > (inflation)!!! >> >> Robyn >> OHSU >> >> >>>>> "Patrick Laurie" 3/20/2007 3:42 PM >>> >>>>> >> >> I completely agree with one of my coworkers, who said that he isn't >> worried until it can cut one of the difficult decals that he skillfully >> cut this morning. I, for one, am looking forward to servicing and >> maintaining our robot overlords. >> >> >> >> >> >> Patrick Laurie, HT (ASCP) >> Neurogenomics Laboratory >> Benaroya Research Institute >> 1201 9th Ave >> Seattle, Wa 98101 >> (206) 341-0681 >> >> Hi, >> >> >> >> >> >> >> >> Has anyone seen the Auto Slide Preparation System AS-200? >> >> >> >> It can replaces a Histotech! >> >> >> >> It can cut and stain. >> >> >> >> The company name is Kurabo Bio-Medical. >> >> >> >> http://www.bio.kurabo.co.jp/English/as200.html >> >> >> >> >> >> >> >> Bye, Bye, >> >> >> >> Carol Ann Bobrowitz >> >> >> >> MCW >> >> >> >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > -- > Victor Tobias > Clinical Applications Analyst > University of Washington Medical Center > Dept of Pathology Room BB220 > 1959 NE Pacific > Seattle, WA 98195 > victor@pathology.washington.edu > 206-598-2792 > 206-598-7659 Fax > ================================================= > Privileged, confidential or patient identifiable information may be > contained in this message. This information is meant only for the use > of the intended recipients. If you are not the intended recipient, or > if the message has been addressed to you in error, do not read, > disclose, reproduce, distribute, disseminate or otherwise use this > transmission. Instead, please notify the sender by reply e-mail, and > then destroy all copies of the message and any attachments. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cgfields <@t> lexhealth.org Thu Mar 22 08:34:58 2007 From: cgfields <@t> lexhealth.org (Carole Fields) Date: Thu Mar 22 08:35:25 2007 Subject: [Histonet] delivery time of last tray of H&E Message-ID: We come in starting at 2 am to embed. The techs cutting are here starting at 4am and ... all are here by 6:30 so all seven docs have slides by 7-7:30 and we are usually finished by 9am. We run 250-325 blocks daily. We have 6 techs cutting and one labeling and distributing slides. The grossing area is here until 6pm and we run 3 VIPs ....loading the first one at noon -30ish so the embedders have work when they come in at 2am. The second machine is loaded at 3 or 4pm and the last is loaded when gross is finished by 6pm. If we had 300 blocks we have about 600 slides. We do have a good team that works well together and they work hard to get the work out and still have nice sections. Hope this helps Carole Fields, HT,ASCP Pathology Supervisor Lexington Medical Center 2720 Sunset Blvd. W. Columbia, SC 29169 -----Original Message----- From: Galiotto, Laura [mailto:LGaliotto@nch.org] Sent: Wednesday, March 21, 2007 4:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] delivery time of last tray of H&E Hello Fellow Histo Tech's, Will anyone be willing to share the delivery time of the last tray of H&E for the days workload to the pathologist reading them? What is your cutoff time for specimen acceptance? What is your annual case workload? I am trying to improve the TAT from specimen gross to the H&E delivered to the pathologist. Unless we change the cutoff time I can not see how I can do this? Any suggestions? We process 22,000 cases a year an average of 280 blks a day consisting of types of tissue. Please Help Laura ******************* PLEASE NOTE ******************* This E-Mail/telefax message and any documents accompanying this transmission may contain information that is privileged, confidential, and/or exempt from disclosure under applicable law and is intended solely for the addressee(s) named above. If you are not the intended addressee/recipient, you are hereby notified that any use of, disclosure, copying, distribution, or reliance on the contents of this E-Mail/telefax information is strictly prohibited and may result in legal action against you. Please reply to the sender advising of the error in transmission and immediately delete/destroy the message and any accompanying documents. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. From histology <@t> gradymem.org Thu Mar 22 09:04:22 2007 From: histology <@t> gradymem.org (histology@gradymem.org) Date: Thu Mar 22 09:04:30 2007 Subject: [Histonet] Protein U on Breast Panel ??? Message-ID: We had a surgeon order a breast panel with Her2Neu, IR-PR, Ploidy, and Protein U. What is "Protein U"? We send out for these test but the place we send them hasn't heard of Protein U. Can anyone shed a light on this for me? Angie Barnett, HTL(ASCP) Grady Memorial Hospital Pathology Department 405/224-2258 histology@gradymem.org From pmcardle <@t> ebsciences.com Thu Mar 22 09:18:12 2007 From: pmcardle <@t> ebsciences.com (Phil McArdle) Date: Thu Mar 22 09:18:23 2007 Subject: [Histonet] Protein U on Breast Panel ??? In-Reply-To: References: Message-ID: <46029024.9080206@ebsciences.com> Hi: While I have heard of a nuclear (or ribonuclear) "Protein U," this is a long shot, but perhaps they want the serum protein (c-erB2) expressed in U/mg or U/mL? Phil McArdle -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Mobile: 860.597.6796 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it, and it was probably my mistake anyway. So please do the right thing and make this e-mail go away. Thank you. histology@gradymem.org wrote: > We had a surgeon order a breast panel with Her2Neu, IR-PR, Ploidy, and > Protein U. > What is "Protein U"? We send out for these test but the place we send > them hasn't heard of Protein U. > Can anyone shed a light on this for me? > > Angie Barnett, HTL(ASCP) > Grady Memorial Hospital > Pathology Department > 405/224-2258 > histology@gradymem.org > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From eileen.lonergan <@t> verizon.net Thu Mar 22 09:25:29 2007 From: eileen.lonergan <@t> verizon.net (eileen.lonergan@verizon.net) Date: Thu Mar 22 09:25:43 2007 Subject: [Histonet] background staining with ultra view from Ventana Message-ID: <12977865.3456751174573535197.JavaMail.root@vms071.mailsrvcs.net> Yes. I just got in 2 new Benchmarks and started with the UltraView. I had Benchmarks before and used the iView with no background. I am considering a switchback to iView if I can't clear this up. I am calling Ventana today on this issue. I thought I was crazy, so thanks for the posting. If anyone else is experiencing this, please keep in touch. Eileen Lonergan Histopathology Manager ConVerge Diagnostic Services 200 Corporate Place Peabody, MA elonergan@convergedx.com (978) 548-5206 >From: Joe Nocito >Date: 2007/03/21 Wed PM 05:20:34 CDT >To: gu.lang@gmx.at, histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] background staining with ultra view from Ventana >oh no, not again!! Last time I answered a question regarding this, I almost >lost my job and was told to get off the Histonet. Nah, not worth it. I'll >keep my mouth shut this time. > See, you can teach an ole dog new tricks. > >JTT > >----- Original Message ----- >From: "Gudrun Lang" >To: >Sent: Wednesday, March 21, 2007 11:09 AM >Subject: [Histonet] background staining with ultra view from Ventana > > >> Hi to the Ventana users, >> >> Have any of you experienced increased background staining with the >> ultraview-DAB kit from Ventana in comparison to the iview-DAB. We have >> problems with this and I would like to know, if it is due to the reagens >> or >> to the machine (Benchmark XT). >> >> >> >> Thanks in advance >> >> Gudrun Lang >> >> > > >-------------------------------------------------------------------------------- > > >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Eileen Lonergan (207) 749-9617 cell (207) 324-6468 home From Jacqueline.Malam <@t> rli.mbht.nhs.uk Thu Mar 22 09:25:20 2007 From: Jacqueline.Malam <@t> rli.mbht.nhs.uk (Malam Jacqueline) Date: Thu Mar 22 09:27:39 2007 Subject: [Histonet] Ig kappa with Benchmark XT Message-ID: Hi Has anyone out there got a good protocol for demonstrating Ig kappa and lambda by IHC on the Ventana Benchmark XT using Dako's antisera codes A1091 and A 1093 and what is the dilution? I will willingly swap any of my protocols in exchange. The UKNEQAS-ICC do not like the ISH protocol we use (ISH protease 3 for 32 minutes) plus we are consistently getting needle-like deposition of the iView blue chromogen despite excluding water from our dehydrating reagents. I will be trying their ISH protease 2 shortly with the ISH protocol so that may solve my problem but any info in the meantime would be most welcome. Jacqui Lancaster uk DISCLAIMER: This e-mail is confidential and privileged. If you are not the intended recipient please accept our apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform postmaster@rli.mbht.nhs.uk that this message has gone astray before deleting it. Comments or opinions expressed in this email are those of their respective contributors only. The views expressed do not represent the views of the Trust, its management or employees. University Hospitals of Morecambe Bay NHS Trust is not responsible and disclaims any and all liability for the content of comments written within.Thank you for your co-operation. From jhr1x <@t> clinmed.gla.ac.uk Thu Mar 22 09:40:06 2007 From: jhr1x <@t> clinmed.gla.ac.uk (Jim Reilly) Date: Thu Mar 22 09:40:15 2007 Subject: [Histonet] Decalc Solutions Message-ID: <004d01c76c8f$fc10c8b0$1c7ed182@ssd4.gla.ac.uk> Hello histonet world Has anyone had experience of using ImmunocalT for decalcifying bone for immunohistochemistry? Jim Reilly From gu.lang <@t> gmx.at Thu Mar 22 10:31:46 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Mar 22 10:31:56 2007 Subject: [Histonet] Re:background staining with ultra view from Ventana In-Reply-To: <12977865.3456751174573535197.JavaMail.root@vms071.mailsrvcs.net> Message-ID: <001e01c76c97$33ff2850$6412a8c0@dielangs.at> The today's run was better than yesterday but background was still there. I have cleaned the Benchy inside, but didn't change anything with the protocols. Now I have swiched on the "ultra-wash" in the protocols. I have overlooked this option and hope, that tomorrow I will have a cleaner result. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: eileen.lonergan@verizon.net [mailto:eileen.lonergan@verizon.net] Gesendet: Donnerstag, 22. M?rz 2007 15:25 An: Joe Nocito; gu.lang@gmx.at; histonet@lists.utsouthwestern.edu Betreff: Re: Re: [Histonet] background staining with ultra view from Ventana Yes. I just got in 2 new Benchmarks and started with the UltraView. I had Benchmarks before and used the iView with no background. I am considering a switchback to iView if I can't clear this up. I am calling Ventana today on this issue. I thought I was crazy, so thanks for the posting. If anyone else is experiencing this, please keep in touch. Eileen Lonergan Histopathology Manager ConVerge Diagnostic Services 200 Corporate Place Peabody, MA elonergan@convergedx.com (978) 548-5206 >From: Joe Nocito >Date: 2007/03/21 Wed PM 05:20:34 CDT >To: gu.lang@gmx.at, histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] background staining with ultra view from Ventana >oh no, not again!! Last time I answered a question regarding this, I almost >lost my job and was told to get off the Histonet. Nah, not worth it. I'll >keep my mouth shut this time. > See, you can teach an ole dog new tricks. > >JTT > >----- Original Message ----- >From: "Gudrun Lang" >To: >Sent: Wednesday, March 21, 2007 11:09 AM >Subject: [Histonet] background staining with ultra view from Ventana > > >> Hi to the Ventana users, >> >> Have any of you experienced increased background staining with the >> ultraview-DAB kit from Ventana in comparison to the iview-DAB. We have >> problems with this and I would like to know, if it is due to the reagens >> or >> to the machine (Benchmark XT). >> >> >> >> Thanks in advance >> >> Gudrun Lang >> >> > > >--------------------------------------------------------------------------- ----- > > >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Eileen Lonergan (207) 749-9617 cell (207) 324-6468 home From dlcowie <@t> prodigy.net Thu Mar 22 10:50:58 2007 From: dlcowie <@t> prodigy.net (Dawn Cowie) Date: Thu Mar 22 10:51:07 2007 Subject: [Histonet] delivery time of last tray of H&E In-Reply-To: Message-ID: <278576.91140.qm@web81002.mail.mud.yahoo.com> We also stagger our tissue processors. The first 1 ends at 10:30pm then midnight and finally 4am. We gross until about 7:30pm. We have a wide variety of tissue types every day. We separate small bx's from large tissues and process the bx's on a short run. 2 techs start at 10:30pm, 1 at midnight and 1 at 7am. We average 450 - 500 blocks a day. We are short staffed at this time with only 4 techs and myself (supv) embedding and cutting. We also have 2 full time lab aides that match up slides to gross dictation, label and distribute. First lab aide starts at 4am and 2nd at 6am. We are usually finished cutting around 8am and last slides are delivered to docs around 10 to 10:am. After that we start recuts and IHC's. We run about 40 - 50 IHC slides each day. It's a big challenge to get this much work out in a short time when you're so short staffed but we also have a very good team and a great bunch of pathologists - this really makes a big difference Hope this info is useful. Oh, and by the way - we're looking for a good tech. Anybody interested? Weather's beautiful here in northwest Florida and the housing market is the best it's been in a long time. Dawn L. Cowie, HT (ASCP) Pensacola Pathologists 5150 Bayou Blvd. Suite 2C Pensacola, Florida 32503 850-416-7251 Carole Fields wrote: We come in starting at 2 am to embed. The techs cutting are here starting at 4am and ... all are here by 6:30 so all seven docs have slides by 7-7:30 and we are usually finished by 9am. We run 250-325 blocks daily. We have 6 techs cutting and one labeling and distributing slides. The grossing area is here until 6pm and we run 3 VIPs ....loading the first one at noon -30ish so the embedders have work when they come in at 2am. The second machine is loaded at 3 or 4pm and the last is loaded when gross is finished by 6pm. If we had 300 blocks we have about 600 slides. We do have a good team that works well together and they work hard to get the work out and still have nice sections. Hope this helps Carole Fields, HT,ASCP Pathology Supervisor Lexington Medical Center 2720 Sunset Blvd. W. Columbia, SC 29169 -----Original Message----- From: Galiotto, Laura [mailto:LGaliotto@nch.org] Sent: Wednesday, March 21, 2007 4:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] delivery time of last tray of H&E Hello Fellow Histo Tech's, Will anyone be willing to share the delivery time of the last tray of H&E for the days workload to the pathologist reading them? What is your cutoff time for specimen acceptance? What is your annual case workload? I am trying to improve the TAT from specimen gross to the H&E delivered to the pathologist. Unless we change the cutoff time I can not see how I can do this? Any suggestions? We process 22,000 cases a year an average of 280 blks a day consisting of types of tissue. Please Help Laura ******************* PLEASE NOTE ******************* This E-Mail/telefax message and any documents accompanying this transmission may contain information that is privileged, confidential, and/or exempt from disclosure under applicable law and is intended solely for the addressee(s) named above. If you are not the intended addressee/recipient, you are hereby notified that any use of, disclosure, copying, distribution, or reliance on the contents of this E-Mail/telefax information is strictly prohibited and may result in legal action against you. Please reply to the sender advising of the error in transmission and immediately delete/destroy the message and any accompanying documents. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Mar 22 11:47:38 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Mar 22 11:47:45 2007 Subject: [Histonet] About the Kurabo AS-200 sectoining instrument. Message-ID: <409659.32291.qm@web61220.mail.yahoo.com> Dear Colleagues: Yesterday I e-mailed Kurabo Industries Ltd. asking for additional information about the AS-200 instrument and today I received their answer. Mr. Hiroomi Ipposhi from the Bio-Medical Department sent me a "pdf" that pritty much says the same thing as was posted. The instrument can prepare 200 sections (1 per slide) from 1 slide in 2 hours or 10 slides with 1 section each from 20 blocks in the same 2 hours for an average of 10 blocks/hour. The sections are transferred on a film. The national sectioning productivity average ranges from 17 to 70 blocks/hour with an average of 31 blocks/hour, which means that this instrument's productivity is 58% of the lower end productivity, 14% of the upper end, and just 32% of the overall average. Starts sectioning at a pre-determined depth so its main application would be on large specimens several mm thick to produce probably control slides. I don't think could be in any way be used for the tiny biopsies received daily in the histology lab. Kurabo Bio-Medical can be reached at: bio@ad.kurabo.co.jp Phone:+81-6-6266-501l Osaka, Japan. It is a textiles manufacturing company founded more than 100 years ago during the Meiji Revolution that started diversifiying into biomedical instruments only a few years ago. I thought you may be interested in knowing! Ren? J. --------------------------------- Need Mail bonding? Go to the Yahoo! Mail Q&A for great tips from Yahoo! Answers users. From rjbuesa <@t> yahoo.com Thu Mar 22 11:52:41 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Mar 22 11:52:46 2007 Subject: [Histonet] delivery time of last tray of H&E In-Reply-To: <270614B321ACB44D8C1D91F4F921FDC304B163C5@NCH01EX02.nch.org> Message-ID: <244247.35699.qm@web61220.mail.yahoo.com> Laura: For a laboratory I used to manage with quite similar annual workload we had the following schedule: 1- last tissue accepted at 7:00 PM; 2- two VIPs atarting with delay to be finished at 4:00 AM next day; 3- Rushes ready for the pathologists at 8:00 AM next day; 4-All slides ready, the latest, at 1:00 PM. 5- All IHC ready by 2 PM 6- all HC ready by noon. Ren? J. "Galiotto, Laura" wrote: Hello Fellow Histo Tech's, Will anyone be willing to share the delivery time of the last tray of H&E for the days workload to the pathologist reading them? What is your cutoff time for specimen acceptance? What is your annual case workload? I am trying to improve the TAT from specimen gross to the H&E delivered to the pathologist. Unless we change the cutoff time I can not see how I can do this? Any suggestions? We process 22,000 cases a year an average of 280 blks a day consisting of types of tissue. Please Help Laura ******************* PLEASE NOTE ******************* This E-Mail/telefax message and any documents accompanying this transmission may contain information that is privileged, confidential, and/or exempt from disclosure under applicable law and is intended solely for the addressee(s) named above. If you are not the intended addressee/recipient, you are hereby notified that any use of, disclosure, copying, distribution, or reliance on the contents of this E-Mail/telefax information is strictly prohibited and may result in legal action against you. Please reply to the sender advising of the error in transmission and immediately delete/destroy the message and any accompanying documents. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Sucker-punch spam with award-winning protection. Try the free Yahoo! Mail Beta. From George.Adams <@t> UTSouthwestern.edu Thu Mar 22 12:10:20 2007 From: George.Adams <@t> UTSouthwestern.edu (George Adams) Date: Thu Mar 22 12:10:38 2007 Subject: [Histonet] use of anti-chondroitin sulfate antibody CS56 with chondroitinase-treated samples Message-ID: <4602722C0200001800014ADC@swnw126.swmed.edu> Histonetters: Thanks for helping me out on recent queries. Has anyone tested the use of the anti-chondroitin sulfate antibody CS56 (available from Sigma, GeneTex among others) with tissues that have been treated with chondroitinase ABC and subsequently viewed by immunohistochemistry (IHC)?. Please note "ABC" refers to the specific isoform of the enzyme chondroitinase, not avidin biotin complex! Is binding with this antibody abolished or does the chondroitinase treatment make no difference (i.e., eptiope not destroyed with treatment)? If anyone has seen no difference in treated vs. untreated (control) samples with this antibody, can you recommend an Ab to chondroitin sulfate where I will see a difference? I have found one Ab to the NG2 chondroitin sulfate that is supposed to bind "mature chondroitin sulfate proteoglycan core glycoprotein as well asprecursor peptides of 210, 220 and 240 kDa" according to US Biological. Has anyone had experience with this particular Ab? George Adams UT Southwestern Medical Center From mlgiebel <@t> vcu.edu Thu Mar 22 12:11:47 2007 From: mlgiebel <@t> vcu.edu (Mary L Giebel/FS/VCU) Date: Thu Mar 22 12:11:56 2007 Subject: [Histonet] About the Kurabo AS-200 sectioning instrument. Message-ID: Has anyone found out the price in US dollars including setup and shipping? -----his To: histonet@lists.utsouthwest From: Rene J Buesa Sent by: histone Date: 03/22/2007 11:47AM Subject: Dear Collea Yesterday I e-mailed Kurabo Industries Ltd. asking for addit information about the AS-200 instrument and today I received their an swer. Mr. Hiroomi Ipposhi from the Bio-Medical Department sent me that pritty much says the same thing as was posted. The in in 2 hours or same 2 hours for an ave transferred on a film. Th blocks/hour instrument's prod of the upper end, and ju Starts sectioning at a pre-determin would be on large specimens several mm thi control slides. I don't think could be in a received daily in the histology lab. Kurabo Bio-Medical can be reached at: bio@ad.kurabo.co.jp It is a textiles manufac ago during the Meiji Revolution biomedical instruments only a few years ago I thought you may be interested in knowing! Ren? J. --------------------------------- Need Mail bonding?Go to the Yahoo! Mail Q&A for great tips from Yahoo! Answers users._____________________ 5F__ _______________________ Hi Histonet@lists.utsouthwestern.edu [1]htt References 1. file://localhost/tmp/3D"http From rjbuesa <@t> yahoo.com Thu Mar 22 12:23:27 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Mar 22 12:23:40 2007 Subject: [Histonet] About the Kurabo AS-200 sectioning instrument. In-Reply-To: Message-ID: <681189.62630.qm@web61221.mail.yahoo.com> Kurabo Ltd. will exhibit in Tampa at ABRF 2007 from 1 to 3 April, but NOT this instrument. Ren? J. Mary L Giebel/FS/VCU wrote: Has anyone found out the price in US dollars including setup and shipping? -----histonet-bounces@lists.utsouthwestern.edu wrote: ----- To: histonet@lists.utsouthwestern.edu From: Rene J Buesa Sent by: histonet-bounces@lists.utsouthwestern.edu Date: 03/22/2007 11:47AM Subject: [Histonet] About the Kurabo AS-200 sectoining instrument. Dear Colleagues: Yesterday I e-mailed Kurabo Industries Ltd. asking for additional information about the AS-200 instrument and today I received their answer. Mr. Hiroomi Ipposhi from the Bio-Medical Department sent me a "pdf" that pritty much says the same thing as was posted. The instrument can prepare 200 sections (1 per slide) from 1 slide in 2 hours or 10 slides with 1 section each from 20 blocks in the same 2 hours for an average of 10 blocks/hour. The sections are transferred on a film. The national sectioning productivity average ranges from 17 to 70 blocks/hour with an average of 31 blocks/hour, which means that this instrument's productivity is 58% of the lower end productivity, 14% of the upper end, and just 32% of the overall average. Starts sectioning at a pre-determined depth so its main application would be on large specimens several mm thick to produce probably control slides. I don't think could be in any way be used for the tiny biopsies received daily in the histology lab. Kurabo Bio-Medical can be reached at: bio@ad.kurabo.co.jp Phone:+81-6-6266-501l Osaka, Japan. It is a textiles manufacturing company founded more than 100 years ago during the Meiji Revolution that started diversifiying into biomedical instruments only a few years ago. I thought you may be interested in knowing! Ren? J. --------------------------------- Need Mail bonding? Go to the Yahoo! Mail Q&A for great tips from Yahoo! Answers users. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Don't pick lemons. See all the new 2007 cars at Yahoo! Autos. From akemiat3377 <@t> yahoo.com Thu Mar 22 12:24:35 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Thu Mar 22 12:24:43 2007 Subject: [Histonet] delivery time of last tray of H&E In-Reply-To: <244247.35699.qm@web61220.mail.yahoo.com> Message-ID: <409001.76905.qm@web31308.mail.mud.yahoo.com> Ren?--That was similar to the protocol I followed in the old days before 24/6 . We provided rush's 1st, followed by bx's by 8AM, we did however, provide special stains and recuts and IHC's by 2-3 PM, depending if it wasn't a lengthy procedure. If you have off site facilities to provide for, that is a different situation. You have to work around courier sheduals for delivering to off site facilities. Everyone needs their slides and time is money!! With the new microwave processing technology that is now in use, there has been a shift to having the majority of staff starting at 10 PM at night. MUCH TO THE DISLIKING OF HISTOTECH"S. The day shift is minimum. IHC is done during the day shift, after the pathologist has determined to do so. In most situations, a battery of IHC's tests are pre-set, as well as with special stains. Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting & Staffing Specializing in Histology, SS, IHC, & Microarray Madison, WI Tele: (925) 788-0900 E-Mail: akemiat3377@yahoo.com --- Rene J Buesa wrote: > Laura: > For a laboratory I used to manage with quite > similar annual workload we had the following > schedule: > 1- last tissue accepted at 7:00 PM; > 2- two VIPs atarting with delay to be finished at > 4:00 AM next day; > 3- Rushes ready for the pathologists at 8:00 AM > next day; > 4-All slides ready, the latest, at 1:00 PM. > 5- All IHC ready by 2 PM > 6- all HC ready by noon. > Ren? J. > > "Galiotto, Laura" wrote: > Hello Fellow Histo Tech's, > Will anyone be willing to share the delivery time of > the last tray of H&E for the days workload to the > pathologist reading them? > What is your cutoff time for specimen acceptance? > What is your annual case workload? > I am trying to improve the TAT from specimen gross > to the H&E delivered to the pathologist. Unless we > change the cutoff time I can not see how I can do > this? Any suggestions? > We process 22,000 cases a year an average of 280 > blks a day consisting of types of tissue. > Please Help > Laura > > > ******************* PLEASE NOTE ******************* > This E-Mail/telefax message and any documents > accompanying this transmission may contain > information that is privileged, confidential, and/or > exempt from disclosure under applicable law and is > intended solely for the addressee(s) named above. If > you are not the intended addressee/recipient, you > are hereby notified that any use of, disclosure, > copying, distribution, or reliance on the contents > of this E-Mail/telefax information is strictly > prohibited and may result in legal action against > you. Please reply to the sender advising of the > error in transmission and immediately delete/destroy > the message and any accompanying documents. Thank > you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Sucker-punch spam with award-winning protection. > Try the free Yahoo! Mail Beta. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Terry.Marshall <@t> rothgen.nhs.uk Thu Mar 22 12:28:03 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Thu Mar 22 12:28:20 2007 Subject: [Histonet] delivery time of last tray of H&E Message-ID: <407F05A128805F4C879A33DBA32E618E20C2A3@TRFT-EX01.xRothGen.nhs.uk> This is all bloody unbelievable. Don't any of you sleep? What is the point of working at night? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: 22 March 2007 17:25 To: Rene J Buesa; Galiotto, Laura; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] delivery time of last tray of H&E Ren?--That was similar to the protocol I followed in the old days before 24/6 . We provided rush's 1st, followed by bx's by 8AM, we did however, provide special stains and recuts and IHC's by 2-3 PM, depending if it wasn't a lengthy procedure. If you have off site facilities to provide for, that is a different situation. You have to work around courier sheduals for delivering to off site facilities. Everyone needs their slides and time is money!! With the new microwave processing technology that is now in use, there has been a shift to having the majority of staff starting at 10 PM at night. MUCH TO THE DISLIKING OF HISTOTECH"S. The day shift is minimum. IHC is done during the day shift, after the pathologist has determined to do so. In most situations, a battery of IHC's tests are pre-set, as well as with special stains. Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting & Staffing Specializing in Histology, SS, IHC, & Microarray Madison, WI Tele: (925) 788-0900 E-Mail: akemiat3377@yahoo.com --- Rene J Buesa wrote: > Laura: > For a laboratory I used to manage with quite similar annual workload > we had the following > schedule: > 1- last tissue accepted at 7:00 PM; > 2- two VIPs atarting with delay to be finished at 4:00 AM next day; > 3- Rushes ready for the pathologists at 8:00 AM next day; > 4-All slides ready, the latest, at 1:00 PM. > 5- All IHC ready by 2 PM > 6- all HC ready by noon. > Ren? J. > > "Galiotto, Laura" wrote: > Hello Fellow Histo Tech's, > Will anyone be willing to share the delivery time of the last tray of > H&E for the days workload to the pathologist reading them? > What is your cutoff time for specimen acceptance? > What is your annual case workload? > I am trying to improve the TAT from specimen gross to the H&E > delivered to the pathologist. Unless we change the cutoff time I can > not see how I can do this? Any suggestions? > We process 22,000 cases a year an average of 280 blks a day consisting > of types of tissue. > Please Help > Laura > > > ******************* PLEASE NOTE ******************* This > E-Mail/telefax message and any documents accompanying this > transmission may contain information that is privileged, confidential, > and/or exempt from disclosure under applicable law and is intended > solely for the addressee(s) named above. If you are not the intended > addressee/recipient, you are hereby notified that any use of, > disclosure, copying, distribution, or reliance on the contents of this > E-Mail/telefax information is strictly prohibited and may result in > legal action against you. Please reply to the sender advising of the > error in transmission and immediately delete/destroy the message and > any accompanying documents. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Sucker-punch spam with award-winning protection. > Try the free Yahoo! Mail Beta. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From KMENDELL <@t> nhs-healthlink.org Thu Mar 22 12:45:34 2007 From: KMENDELL <@t> nhs-healthlink.org (Kate Mendell) Date: Thu Mar 22 12:45:54 2007 Subject: [Histonet] BerEP4 Message-ID: <4602887E020000A800012BC8@mail.NHS-HEALTHLINK.ORG> I was wondering if anyone is using this antibody. I am working it up and using Skin as control but it's not staining. Any suggestions. Anyone want to share their protocol. My product is from Dako Kate ??is message and its contents are confidential and are intended for the use of the addressee only, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the intended recipient, this serves as notice that any unauthorized distribution, duplication, printing, or any other use is strictly prohibited. If you feel you have received this email in error, please delete the message and notify the sender so that we may prevent future occurrences. From David.Edmondson <@t> christie-tr.nwest.nhs.uk Thu Mar 22 12:47:10 2007 From: David.Edmondson <@t> christie-tr.nwest.nhs.uk (Edmondson David (RBV) NHS Christie Tr) Date: Thu Mar 22 12:49:21 2007 Subject: [Histonet] BerEP4 References: <4602887E020000A800012BC8@mail.NHS-HEALTHLINK.ORG> Message-ID: <8B54F5975FC1314BBE8105F8FFC595FD2EC48D@cht-mail3-2k.xchristie.nhs.uk> We would recommend Trypsinisation and using a section of Ileum as control material and the Dako Ab will come up a treat Dave Christie Hosp, Manchester. UK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kate Mendell Sent: 22 March 2007 17:46 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] BerEP4 I was wondering if anyone is using this antibody. I am working it up and using Skin as control but it's not staining. Any suggestions. Anyone want to share their protocol. My product is from Dako Kate ??is message and its contents are confidential and are intended for the use of the addressee only, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the intended recipient, this serves as notice that any unauthorized distribution, duplication, printing, or any other use is strictly prohibited. If you feel you have received this email in error, please delete the message and notify the sender so that we may prevent future occurrences. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************** This e-mail and any files transmitted with it are confidential and solely for the use of the intended recipient. If you have received this e-mail in error you should not disseminate, distribute or copy it. Please notify the sender immediately and delete this e-mail from your system. ******************************************************************************************************************* From doug <@t> ppspath.com Thu Mar 22 13:54:23 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Thu Mar 22 12:52:45 2007 Subject: [Histonet] delivery time of last tray of H&E In-Reply-To: <407F05A128805F4C879A33DBA32E618E20C2A3@TRFT-EX01.xRothGen.nhs.uk> Message-ID: I don't get it either. We have a little histo pixies that comes in while we sleep and the slides magically appear on the pathologist desk in the morning. :) Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Thursday, March 22, 2007 12:28 PM To: Akemi Allison-Tacha; Rene J Buesa; Galiotto, Laura; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] delivery time of last tray of H&E This is all bloody unbelievable. Don't any of you sleep? What is the point of working at night? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: 22 March 2007 17:25 To: Rene J Buesa; Galiotto, Laura; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] delivery time of last tray of H&E Ren?--That was similar to the protocol I followed in the old days before 24/6 . We provided rush's 1st, followed by bx's by 8AM, we did however, provide special stains and recuts and IHC's by 2-3 PM, depending if it wasn't a lengthy procedure. If you have off site facilities to provide for, that is a different situation. You have to work around courier sheduals for delivering to off site facilities. Everyone needs their slides and time is money!! With the new microwave processing technology that is now in use, there has been a shift to having the majority of staff starting at 10 PM at night. MUCH TO THE DISLIKING OF HISTOTECH"S. The day shift is minimum. IHC is done during the day shift, after the pathologist has determined to do so. In most situations, a battery of IHC's tests are pre-set, as well as with special stains. Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting & Staffing Specializing in Histology, SS, IHC, & Microarray Madison, WI Tele: (925) 788-0900 E-Mail: akemiat3377@yahoo.com --- Rene J Buesa wrote: > Laura: > For a laboratory I used to manage with quite similar annual workload > we had the following > schedule: > 1- last tissue accepted at 7:00 PM; > 2- two VIPs atarting with delay to be finished at 4:00 AM next day; > 3- Rushes ready for the pathologists at 8:00 AM next day; > 4-All slides ready, the latest, at 1:00 PM. > 5- All IHC ready by 2 PM > 6- all HC ready by noon. > Ren? J. > > "Galiotto, Laura" wrote: > Hello Fellow Histo Tech's, > Will anyone be willing to share the delivery time of the last tray of > H&E for the days workload to the pathologist reading them? > What is your cutoff time for specimen acceptance? > What is your annual case workload? > I am trying to improve the TAT from specimen gross to the H&E > delivered to the pathologist. Unless we change the cutoff time I can > not see how I can do this? Any suggestions? > We process 22,000 cases a year an average of 280 blks a day consisting > of types of tissue. > Please Help > Laura > > > ******************* PLEASE NOTE ******************* This > E-Mail/telefax message and any documents accompanying this > transmission may contain information that is privileged, confidential, > and/or exempt from disclosure under applicable law and is intended > solely for the addressee(s) named above. If you are not the intended > addressee/recipient, you are hereby notified that any use of, > disclosure, copying, distribution, or reliance on the contents of this > E-Mail/telefax information is strictly prohibited and may result in > legal action against you. Please reply to the sender advising of the > error in transmission and immediately delete/destroy the message and > any accompanying documents. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Sucker-punch spam with award-winning protection. > Try the free Yahoo! Mail Beta. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sheri.Meilus <@t> va.gov Thu Mar 22 13:00:33 2007 From: Sheri.Meilus <@t> va.gov (Meilus, Sheri D.) Date: Thu Mar 22 13:00:59 2007 Subject: [Histonet] Degree in Histotechnology Message-ID: Dear Histonetters, St. Petersburg College is seriously considering starting AS and BS programs in Histotechnology. I will be attending their next Advisory Committee meeting. They would like the following information: * Number of unfilled HT/HTL positions in your laboratory * Average length of time to fill HT/HTL positions in your laboratory * Projected annual need for new HT/HTL positions in your laboratory * Salary ranges for HT/HTL in your laboratory If as many of you as possible will provide me with this information, I will be glad to represent you at the meeting. Thanks and good luck to all of us! S Sheri Meilus, HT(ASCP)QIHC Anatomic Pathology Supervisor Bay Pines VAMC Bay Pines, FL 33744 727-398-6661 4596 From HoustonR <@t> chi.osu.edu Thu Mar 22 13:07:17 2007 From: HoustonR <@t> chi.osu.edu (Houston, Ronald) Date: Thu Mar 22 13:07:51 2007 Subject: [Histonet] BerEP4 Message-ID: <979FF5962E234F45B06CF0DB7C1AABB20D77D0DF@chi2k3ms01.columbuschildrens.net> 4 minutes proteinase K at room temp, and adenocarcinoma as control - works a treat Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Columbus Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 houstonr@chi.osu.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kate Mendell Sent: Thursday, March 22, 2007 1:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] BerEP4 I was wondering if anyone is using this antibody. I am working it up and using Skin as control but it's not staining. Any suggestions. Anyone want to share their protocol. My product is from Dako Kate ??is message and its contents are confidential and are intended for the use of the addressee only, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the intended recipient, this serves as notice that any unauthorized distribution, duplication, printing, or any other use is strictly prohibited. If you feel you have received this email in error, please delete the message and notify the sender so that we may prevent future occurrences. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: This e-mail message, from Children's Hospital, Columbus, Ohio, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes pursuant to Children's Hospital's confidentiality policies. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From Charles.DiComo <@t> AUREON.com Thu Mar 22 13:19:10 2007 From: Charles.DiComo <@t> AUREON.com (Charles J. DiComo) Date: Thu Mar 22 13:19:54 2007 Subject: [Histonet] Job Posting - Histotechnician Message-ID: <6ED54DA7BF8AAE4194687E4B008687B9CB14DD@aureon-ex02.UNIVERSE.AUREON.COM> At Aureon Laboratories, our mission is to be the leader in personalized predictive pathology. It is our passion to provide patients and physicians with improved decision making tools that enable improved patient care. At Aureon, we use a "Systems Pathology" approach integrating clinical information, biomarkers and computer analysis; taken together we create the most advanced and accurate prediction of an individual's disease available today. Under general supervision, you will perform technical duties in the preparation of surgical biopsy specimens for microscopic examination and prepare histologic slides from tissue sections for microscopic examination and diagnosis by a Pathologist. Successful candidate will gross, process, embed, cut and stain surgical biopsies and perform complex histologic procedures and analyze and correct problems using scientific principles. Must be proficient working with an automated tissue processor, H&E autostainer, cover-slipper, microtome, and embedding stations. Perform accurate, reliable and high level work efficiently, must have good organizational skills with attention to detail, and must communicate well verbally and in writing. Prefer candidate with experience in immunohistochemistry and immunofluorescence. Experience working independently with challenging small biopsy specimens in a specialized diagnostic lab setting a plus. Minimum of 3-5 years full-time experience in Histopathology working in a clinical setting under the direction of a Board Certified Pathologist. Should have ASCP Histotechnician certification. This is a full time position, however per diem opportunities exist. We work in a modern state-of-the-art laboratory with a fully integrated laboratory information system and computer network. We are located one block from the Hudson River Line Metro North Railroad just 20 minutes outside of New York City. For our employees, we offer an innovative environment, comprehensive resources, ongoing career development. We offer all of our colleagues an opportunity to participate in an outstanding benefits package that includes Health, Dental, Disability, 401(k) Retirement Plan, and pre-tax Flexible Spending Accounts. Aureon Laboratories is dedicated to providing the people we serve-patients and physicians with the highest degree of service. If you are seeking a rewarding, challenging career in a laboratory environment with an organization that will develop your strengths and help you meet your personal and professional goals, we want to talk with you. Please submit a summary of experience, CV/Resume, names of three (3) references and salary history to recruiter@aureon.com or fax to 914-377-4001 or mail to: Aureon Laboratories, Inc. 28 Wells Avenue Yonkers, New York 10701 From gu.lang <@t> gmx.at Thu Mar 22 13:51:36 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Mar 22 13:51:44 2007 Subject: AW: [Histonet] delivery time of last tray of H&E In-Reply-To: <407F05A128805F4C879A33DBA32E618E20C2A3@TRFT-EX01.xRothGen.nhs.uk> Message-ID: <000001c76cb3$1e9d2770$6412a8c0@dielangs.at> Lucky Austrian histotechs. The earliest beginning is 5am I think. My lab is medium in range with 150 to 250 blocks a day. One histotech begins at 6.30 with embedding. We deliver our first slides at 9.30. The last HE is usually delivered before noon. There are three pathologists, who look at the slides until afternoon. And we do the recuts and preperations for IHC parallel. Grossing is from 11am until 3pm, with breaks. My personel shift is from 7.30 to 3.30. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Marshall Terry Dr,Consultant Histopathologist Gesendet: Donnerstag, 22. M?rz 2007 18:28 An: Akemi Allison-Tacha; Rene J Buesa; Galiotto, Laura; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] delivery time of last tray of H&E This is all bloody unbelievable. Don't any of you sleep? What is the point of working at night? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: 22 March 2007 17:25 To: Rene J Buesa; Galiotto, Laura; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] delivery time of last tray of H&E Ren?--That was similar to the protocol I followed in the old days before 24/6 . We provided rush's 1st, followed by bx's by 8AM, we did however, provide special stains and recuts and IHC's by 2-3 PM, depending if it wasn't a lengthy procedure. If you have off site facilities to provide for, that is a different situation. You have to work around courier sheduals for delivering to off site facilities. Everyone needs their slides and time is money!! With the new microwave processing technology that is now in use, there has been a shift to having the majority of staff starting at 10 PM at night. MUCH TO THE DISLIKING OF HISTOTECH"S. The day shift is minimum. IHC is done during the day shift, after the pathologist has determined to do so. In most situations, a battery of IHC's tests are pre-set, as well as with special stains. Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting & Staffing Specializing in Histology, SS, IHC, & Microarray Madison, WI Tele: (925) 788-0900 E-Mail: akemiat3377@yahoo.com --- Rene J Buesa wrote: > Laura: > For a laboratory I used to manage with quite similar annual workload > we had the following > schedule: > 1- last tissue accepted at 7:00 PM; > 2- two VIPs atarting with delay to be finished at 4:00 AM next day; > 3- Rushes ready for the pathologists at 8:00 AM next day; > 4-All slides ready, the latest, at 1:00 PM. > 5- All IHC ready by 2 PM > 6- all HC ready by noon. > Ren? J. > > "Galiotto, Laura" wrote: > Hello Fellow Histo Tech's, > Will anyone be willing to share the delivery time of the last tray of > H&E for the days workload to the pathologist reading them? > What is your cutoff time for specimen acceptance? > What is your annual case workload? > I am trying to improve the TAT from specimen gross to the H&E > delivered to the pathologist. Unless we change the cutoff time I can > not see how I can do this? Any suggestions? > We process 22,000 cases a year an average of 280 blks a day consisting > of types of tissue. > Please Help > Laura > > > ******************* PLEASE NOTE ******************* This > E-Mail/telefax message and any documents accompanying this > transmission may contain information that is privileged, confidential, > and/or exempt from disclosure under applicable law and is intended > solely for the addressee(s) named above. If you are not the intended > addressee/recipient, you are hereby notified that any use of, > disclosure, copying, distribution, or reliance on the contents of this > E-Mail/telefax information is strictly prohibited and may result in > legal action against you. Please reply to the sender advising of the > error in transmission and immediately delete/destroy the message and > any accompanying documents. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Sucker-punch spam with award-winning protection. > Try the free Yahoo! Mail Beta. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From japoteete <@t> saintfrancis.com Thu Mar 22 14:01:44 2007 From: japoteete <@t> saintfrancis.com (Poteete, Jacquie A.) Date: Thu Mar 22 14:03:44 2007 Subject: [Histonet] IHC orders Message-ID: Just curious - how many places do IHCs the same day they are requested? What is your cut-off time for same day testing? From Robinsoc <@t> mercyhealth.com Thu Mar 22 14:12:49 2007 From: Robinsoc <@t> mercyhealth.com (Cindy Robinson) Date: Thu Mar 22 14:13:15 2007 Subject: [Histonet] IHC orders In-Reply-To: References: Message-ID: <46028EE1020000AF00000145@nodcmsngwia1.trinity-health.org> We try to do same day TAT for all orders placed by 11AM. After that time we call the ordering pathologist to see if we can put it on when we start the following day at 5AM so they can have it early...usually by 8AM. Cindi Robinson, HT(ASCP) MMC-Sioux City Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 >>> "Poteete, Jacquie A." 3/22/2007 2:01 PM >>> Just curious - how many places do IHCs the same day they are requested? What is your cut-off time for same day testing? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lhadley <@t> iupui.edu Thu Mar 22 14:19:13 2007 From: lhadley <@t> iupui.edu (Baldridge, Lee Ann) Date: Thu Mar 22 14:19:23 2007 Subject: [Histonet] IHC orders In-Reply-To: References: Message-ID: <1E3324ECFB5D5F4DAFEDD17203EEBF21D36022@iu-mssg-mbx105.ads.iu.edu> If we get the request by 9:30am they will get them the same day. Lee Ann Baldridge Clarian Health IU,Methodist,Riley Indpls.,IN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Poteete, Jacquie A. Sent: Thursday, March 22, 2007 3:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC orders Just curious - how many places do IHCs the same day they are requested? What is your cut-off time for same day testing? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charlene.Henry <@t> STJUDE.ORG Thu Mar 22 14:46:10 2007 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Thu Mar 22 14:46:15 2007 Subject: [Histonet] IHC orders. . In-Reply-To: Message-ID: <5CB39BCA5724F349BCB748675C6CA1A2099A206F@SJMEMXMB02.stjude.sjcrh.local> We complete all IHC tests that are ordered by 9:30 the same day and all that come in after 9:30 but before 2:30 are put on overnight runs so they will be completed by 8:00am following morning. Charlene Henry HT (ASCP), QIHC Anatomic Pathology Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Poteete, Jacquie A. Sent: Thursday, March 22, 2007 2:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC orders. . Just curious - how many places do IHCs the same day they are requested? What is your cut-off time for same day testing? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BMolinari <@t> heart.thi.tmc.edu Thu Mar 22 14:47:33 2007 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Thu Mar 22 14:47:37 2007 Subject: [Histonet] Von Kossa Message-ID: Hi, Has anyone run a Von Kossa on a tissue culture? Thanks, Betsy Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) From Shirley_PHUA <@t> hsa.gov.sg Thu Mar 22 15:02:08 2007 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Thu Mar 22 15:06:14 2007 Subject: [Histonet] Shirley is away 23 March 2007 ... Message-ID: I will be out of the office from 23-03-2007 to 23-03-2007. I will return on 26 March 2007. Pathologists : I will process your requests when I return. Otherwise, if urgent, please forward your mail to henry_kyaw@hsa.gov.sg Regrets for the inconveniences caused. From bills <@t> icpmr.wsahs.nsw.gov.au Thu Mar 22 15:38:01 2007 From: bills <@t> icpmr.wsahs.nsw.gov.au (Bill Sinai) Date: Thu Mar 22 15:55:02 2007 Subject: [Histonet] delivery time of last tray of H&E In-Reply-To: <000001c76cb3$1e9d2770$6412a8c0@wsahs.nsw.gov.au> Message-ID: <003601c76cc1$fbee39d0$3967080a@wsahs.nsw.gov.au> Our workload is up to 700 surgical blocks daily (we also process Forensic Pathology tissues), the first H&E available by 8:30am, only on the busiest days or staff shortage days do we still have H&E after 1:00pm. The number of Frozen Sections and Sentinel Node imprints severely impacts on this, some days particularly Wednesday and Thursday we can have up to 5-6 Frozen Sections and the same number of Sentinel Node Imprints. Specimen Dissection, two rooms runs all day from 8:30am till 6:00pm, virtually non-stop. We have one staffer begin at 6:00am another at 6:30am (embedding)and a third at 7:30am (set-up Specimen Dissection) with the rest coming in between 8:00am and 9:30am (Specimen Reception and Microtomy). Recuts begin at 8:30ish and the immunos begin as soon as a full run is available, we do about 180 per day + the overnight runs (approx 50 slides). Special stains we have two runs daily (one staffer) the first begins about 11-11:30am the second about 1-1:30pm with all stains completed before 4:00pm. We have 8 Full time staff and four half time. Keeps us busy. Bill Sinai Chief Hospital Scientist Tissue Pathology ICPMR Westmead Hospital Westmead NSW 2145 Phone 02 9845 7774 Fax 02 9687 2330 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Friday, 23 March 2007 5:52 AM To: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] delivery time of last tray of H&E Lucky Austrian histotechs. The earliest beginning is 5am I think. My lab is medium in range with 150 to 250 blocks a day. One histotech begins at 6.30 with embedding. We deliver our first slides at 9.30. The last HE is usually delivered before noon. There are three pathologists, who look at the slides until afternoon. And we do the recuts and preperations for IHC parallel. Grossing is from 11am until 3pm, with breaks. My personel shift is from 7.30 to 3.30. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Marshall Terry Dr,Consultant Histopathologist Gesendet: Donnerstag, 22. M?rz 2007 18:28 An: Akemi Allison-Tacha; Rene J Buesa; Galiotto, Laura; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] delivery time of last tray of H&E This is all bloody unbelievable. Don't any of you sleep? What is the point of working at night? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: 22 March 2007 17:25 To: Rene J Buesa; Galiotto, Laura; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] delivery time of last tray of H&E Ren?--That was similar to the protocol I followed in the old days before 24/6 . We provided rush's 1st, followed by bx's by 8AM, we did however, provide special stains and recuts and IHC's by 2-3 PM, depending if it wasn't a lengthy procedure. If you have off site facilities to provide for, that is a different situation. You have to work around courier sheduals for delivering to off site facilities. Everyone needs their slides and time is money!! With the new microwave processing technology that is now in use, there has been a shift to having the majority of staff starting at 10 PM at night. MUCH TO THE DISLIKING OF HISTOTECH"S. The day shift is minimum. IHC is done during the day shift, after the pathologist has determined to do so. In most situations, a battery of IHC's tests are pre-set, as well as with special stains. Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting & Staffing Specializing in Histology, SS, IHC, & Microarray Madison, WI Tele: (925) 788-0900 E-Mail: akemiat3377@yahoo.com --- Rene J Buesa wrote: > Laura: > For a laboratory I used to manage with quite similar annual workload > we had the following > schedule: > 1- last tissue accepted at 7:00 PM; > 2- two VIPs atarting with delay to be finished at 4:00 AM next day; > 3- Rushes ready for the pathologists at 8:00 AM next day; > 4-All slides ready, the latest, at 1:00 PM. > 5- All IHC ready by 2 PM > 6- all HC ready by noon. > Ren? J. > > "Galiotto, Laura" wrote: > Hello Fellow Histo Tech's, > Will anyone be willing to share the delivery time of the last tray of > H&E for the days workload to the pathologist reading them? > What is your cutoff time for specimen acceptance? > What is your annual case workload? > I am trying to improve the TAT from specimen gross to the H&E > delivered to the pathologist. Unless we change the cutoff time I can > not see how I can do this? Any suggestions? > We process 22,000 cases a year an average of 280 blks a day consisting > of types of tissue. > Please Help > Laura > > > ******************* PLEASE NOTE ******************* This > E-Mail/telefax message and any documents accompanying this > transmission may contain information that is privileged, confidential, > and/or exempt from disclosure under applicable law and is intended > solely for the addressee(s) named above. If you are not the intended > addressee/recipient, you are hereby notified that any use of, > disclosure, copying, distribution, or reliance on the contents of this > E-Mail/telefax information is strictly prohibited and may result in > legal action against you. Please reply to the sender advising of the > error in transmission and immediately delete/destroy the message and > any accompanying documents. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Sucker-punch spam with award-winning protection. > Try the free Yahoo! Mail Beta. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________________________ This electronic message and any attachments may be confidential. If you are not the intended recipient of this message would you please delete the message and any attachments and advise the sender. 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From jnocito <@t> satx.rr.com Thu Mar 22 17:30:15 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Mar 22 17:30:25 2007 Subject: [Histonet] Decalc Solutions References: <004d01c76c8f$fc10c8b0$1c7ed182@ssd4.gla.ac.uk> Message-ID: <003801c76cd1$aa92caa0$11614542@yourxhtr8hvc4p> no, but we use IED from Biocare Medical (1-800-799-9499). My hempath says it's the first time that he gets immunos on bone marrows that really work. JTT ----- Original Message ----- From: "Jim Reilly" To: Sent: Thursday, March 22, 2007 9:40 AM Subject: [Histonet] Decalc Solutions > Hello histonet world > > Has anyone had experience of using ImmunocalT for decalcifying bone for > immunohistochemistry? > > Jim Reilly > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Thu Mar 22 17:31:55 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Mar 22 17:32:00 2007 Subject: [Histonet] IHC orders References: Message-ID: <006501c76cd1$e5e39350$11614542@yourxhtr8hvc4p> we take requests up until 3:00 PM, after that, they get run the next day. JTT ----- Original Message ----- From: "Poteete, Jacquie A." To: Sent: Thursday, March 22, 2007 2:01 PM Subject: [Histonet] IHC orders Just curious - how many places do IHCs the same day they are requested? What is your cut-off time for same day testing? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Annette_hall <@t> pa-ucl.com Thu Mar 22 19:07:21 2007 From: Annette_hall <@t> pa-ucl.com (Annette Hall) Date: Thu Mar 22 19:07:27 2007 Subject: [Histonet] IHC orders Message-ID: <9FC023A4AB52BB4D87DC6456081A822C012B5BA8@mercury.pa-ucl.com> We run 2 stainers and 2 runs on both each day. The first cutoff is 1030 for an Artisan (Special Stains & IHC) run at 1200. A Benchmark run (IHC only) is then loaded with a cutoff time of 1130. Both these machines finish by 1500. Overnight runs are then put on both machines. The cutoff is 1530 and the slides are taken off in the morning and delivered to the pathologists by 0730. Annette J Hall Micro/Cyto/Histo Supervisor United Clinical Labs 205 Bluff Street Dubuque, IA 52001 Phone: 563.556.2010 x131 -----Original Message----- From: Poteete, Jacquie A. [mailto:japoteete@saintfrancis.com] Sent: Thursday, March 22, 2007 2:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC orders Just curious - how many places do IHCs the same day they are requested? What is your cut-off time for same day testing? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From dani_1351 <@t> yahoo.com Thu Mar 22 22:22:13 2007 From: dani_1351 <@t> yahoo.com (Dan Malzahn) Date: Thu Mar 22 22:22:20 2007 Subject: [Histonet] Looking to relocate to Youngstown, Oh area Message-ID: <616543.91894.qm@web62405.mail.re1.yahoo.com> Hello All, Just wondering if anyone knows of any jobs that might be in the Youngstown, OH area. I am looking to relocate to that area and I am having a hard time finding any Histology jobs in that area. Any help would be greatly appreciated. Thankyou --------------------------------- Finding fabulous fares is fun. Let Yahoo! FareChase search your favorite travel sites to find flight and hotel bargains. From koellingr <@t> comcast.net Fri Mar 23 00:47:08 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Fri Mar 23 00:47:16 2007 Subject: [Histonet] Von Kossa Message-ID: <032320070547.16487.460369DC000550CC0000406722070229339D09020704040A0105@comcast.net> Betsy, In case you haven't had specific procedural reply's, I'll say yes. Did it for several years but back where I can't get at notes and 15 years of memory loss. Did VK on my cultures I maintained for project. Performed on 8-well tissue culture slides, both glass and plastic and tissue culture plates of all sizes. The procedure is basically the same as the VK you know-it just needs a bit of inginuity. Anything plastic obviously you can't go through xylenes but that didn't matter as after NFR, washed in water, got a coverlip on somehow with aqueous media and photographed right on plate with inverted scope or slide as usual. Didn't use "the sun" but a great lamp. Make sure you have a good positive control to work up procedure. I made up my own pos and neg tissue culture controls for VK as my experiments went forward. Look in PubMed and find van Griensven M, Exp Toxiicol Pathol 2002 Jul; 54 (1) 25-9. Or the Journal Calcified Tissue International vol 72, Number 5, May 2003 537-547 "Von Kossa Staining alone is not sufficient to confirm that mineralization in vitro represents bone formation; the authors of which (Bonewald, Harris, Rosser, Dallas)came out of Texas Health Sciences Center down your way. There are many other such references in a PubMed search. Would that they had published years earlier so I wouldn't have had so many nights and weekends in the lab. But my thesis adviser honestly did not say there would be much glory and stardom, just lots of hard work. Raymond Koelling Phenopath Laboratories Seattle, WA -------------- Original message -------------- From: "Molinari, Betsy" > Hi, > > Has anyone run a Von Kossa on a tissue culture? > > Thanks, > > Betsy > > > > > > Betsy Molinari HT (ASCP) > > Texas Heart Institute > > Cardiovascular Pathology > > 6770 Bertner Ave. > > Houston,TX 77030 > > 832-355-6524 > > 832-355-6812 (fax) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dlcowie <@t> prodigy.net Fri Mar 23 02:55:34 2007 From: dlcowie <@t> prodigy.net (Dawn Cowie) Date: Fri Mar 23 02:55:39 2007 Subject: [Histonet] IHC orders In-Reply-To: <46028EE1020000AF00000145@nodcmsngwia1.trinity-health.org> Message-ID: <813762.29342.qm@web81007.mail.mud.yahoo.com> IHC requests received by 11am are done the same day. After that they're put on the Benchmark to come off when the night shift arrives and delivered to the docs early next morning. Dawn L. Cowie, HT (ASCP) Histology Supervisor Pensacola Pathologists, PA Pensacola, Florida 32503 850-416-7251 Cindy Robinson wrote: We try to do same day TAT for all orders placed by 11AM. After that time we call the ordering pathologist to see if we can put it on when we start the following day at 5AM so they can have it early...usually by 8AM. Cindi Robinson, HT(ASCP) MMC-Sioux City Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 >>> "Poteete, Jacquie A." 3/22/2007 2:01 PM >>> Just curious - how many places do IHCs the same day they are requested? What is your cut-off time for same day testing? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dlcowie <@t> prodigy.net Fri Mar 23 03:03:46 2007 From: dlcowie <@t> prodigy.net (Dawn Cowie) Date: Fri Mar 23 03:03:50 2007 Subject: [Histonet] delivery time of last tray of H&E In-Reply-To: <200703221756.l2MHusm8011504@nlpi046.sbcis.sbc.com> Message-ID: <20070323080346.3665.qmail@web81001.mail.mud.yahoo.com> We do what we are required to do by the pathologists. I don't know why they think they need all H&E's so early - they can't look at them all at the same time and sign them out - except that they have them just in case a clinician calls. The trend these days is certainly toward faster and faster turnaround time. Dawn L. Cowie, HT (ASCP) Histology Supervisor Pensacola Pathologists, PA Pensacola, Florida 32503 850-416-7251 Douglas D Deltour wrote: I don't get it either. We have a little histo pixies that comes in while we sleep and the slides magically appear on the pathologist desk in the morning. :) Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Thursday, March 22, 2007 12:28 PM To: Akemi Allison-Tacha; Rene J Buesa; Galiotto, Laura; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] delivery time of last tray of H&E This is all bloody unbelievable. Don't any of you sleep? What is the point of working at night? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: 22 March 2007 17:25 To: Rene J Buesa; Galiotto, Laura; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] delivery time of last tray of H&E Ren?--That was similar to the protocol I followed in the old days before 24/6 . We provided rush's 1st, followed by bx's by 8AM, we did however, provide special stains and recuts and IHC's by 2-3 PM, depending if it wasn't a lengthy procedure. If you have off site facilities to provide for, that is a different situation. You have to work around courier sheduals for delivering to off site facilities. Everyone needs their slides and time is money!! With the new microwave processing technology that is now in use, there has been a shift to having the majority of staff starting at 10 PM at night. MUCH TO THE DISLIKING OF HISTOTECH"S. The day shift is minimum. IHC is done during the day shift, after the pathologist has determined to do so. In most situations, a battery of IHC's tests are pre-set, as well as with special stains. Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting & Staffing Specializing in Histology, SS, IHC, & Microarray Madison, WI Tele: (925) 788-0900 E-Mail: akemiat3377@yahoo.com --- Rene J Buesa wrote: > Laura: > For a laboratory I used to manage with quite similar annual workload > we had the following > schedule: > 1- last tissue accepted at 7:00 PM; > 2- two VIPs atarting with delay to be finished at 4:00 AM next day; > 3- Rushes ready for the pathologists at 8:00 AM next day; > 4-All slides ready, the latest, at 1:00 PM. > 5- All IHC ready by 2 PM > 6- all HC ready by noon. > Ren? J. > > "Galiotto, Laura" wrote: > Hello Fellow Histo Tech's, > Will anyone be willing to share the delivery time of the last tray of > H&E for the days workload to the pathologist reading them? > What is your cutoff time for specimen acceptance? > What is your annual case workload? > I am trying to improve the TAT from specimen gross to the H&E > delivered to the pathologist. Unless we change the cutoff time I can > not see how I can do this? Any suggestions? > We process 22,000 cases a year an average of 280 blks a day consisting > of types of tissue. > Please Help > Laura > > > ******************* PLEASE NOTE ******************* This > E-Mail/telefax message and any documents accompanying this > transmission may contain information that is privileged, confidential, > and/or exempt from disclosure under applicable law and is intended > solely for the addressee(s) named above. If you are not the intended > addressee/recipient, you are hereby notified that any use of, > disclosure, copying, distribution, or reliance on the contents of this > E-Mail/telefax information is strictly prohibited and may result in > legal action against you. Please reply to the sender advising of the > error in transmission and immediately delete/destroy the message and > any accompanying documents. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Sucker-punch spam with award-winning protection. > Try the free Yahoo! Mail Beta. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dlcowie <@t> prodigy.net Fri Mar 23 03:24:07 2007 From: dlcowie <@t> prodigy.net (Dawn Cowie) Date: Fri Mar 23 03:24:14 2007 Subject: [Histonet] Degree in Histotechnology In-Reply-To: Message-ID: <20070323082407.59234.qmail@web81009.mail.mud.yahoo.com> Sheri, We have 2 FTE's available now for preferably HTL (Florida licensed). It usually takes us about 6-9 months to fill a position - sometimes longer. I still think the Florida license scares people from out of state even when you explain the rules to them regarding ASCP certification and getting a license. Our HT's salary range is approx. $20-25 per hour. Our HTL's salary range is approx. $22-28 per hour. Both positions salaries dependent on number of years in the field, actual work experience, etc. We will probably need to replace 1, possibly 2 HTL's in the next couple of years due to retirement. The majority of my staff are over 50. We definitely need another histo program in Florida - there aren't enough techs to fill all the positions in this state. Hope my 2 cents helps. Dawn L. Cowie, HT (ASCP) Histology Supervisor Pensacola Pathologists, PA Pensacola, Florida 32503 850-416-7251 "Meilus, Sheri D." wrote: Dear Histonetters, St. Petersburg College is seriously considering starting AS and BS programs in Histotechnology. I will be attending their next Advisory Committee meeting. They would like the following information: * Number of unfilled HT/HTL positions in your laboratory * Average length of time to fill HT/HTL positions in your laboratory * Projected annual need for new HT/HTL positions in your laboratory * Salary ranges for HT/HTL in your laboratory If as many of you as possible will provide me with this information, I will be glad to represent you at the meeting. Thanks and good luck to all of us! S Sheri Meilus, HT(ASCP)QIHC Anatomic Pathology Supervisor Bay Pines VAMC Bay Pines, FL 33744 727-398-6661 4596 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BMolinari <@t> heart.thi.tmc.edu Fri Mar 23 05:42:27 2007 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri Mar 23 05:42:34 2007 Subject: [Histonet] Von Kossa In-Reply-To: <032320070547.16487.460369DC000550CC0000406722070229339D09020704040A0105@comcast.net> Message-ID: Ralph, Thank you so much for your input. Now that I know it has been done and with your advice I feel I can move forward with more confidence. Thanks again. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: koellingr@comcast.net [mailto:koellingr@comcast.net] Sent: Friday, March 23, 2007 12:47 AM To: Molinari, Betsy; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Von Kossa Betsy, In case you haven't had specific procedural reply's, I'll say yes. Did it for several years but back where I can't get at notes and 15 years of memory loss. Did VK on my cultures I maintained for project. Performed on 8-well tissue culture slides, both glass and plastic and tissue culture plates of all sizes. The procedure is basically the same as the VK you know-it just needs a bit of inginuity. Anything plastic obviously you can't go through xylenes but that didn't matter as after NFR, washed in water, got a coverlip on somehow with aqueous media and photographed right on plate with inverted scope or slide as usual. Didn't use "the sun" but a great lamp. Make sure you have a good positive control to work up procedure. I made up my own pos and neg tissue culture controls for VK as my experiments went forward. Look in PubMed and find van Griensven M, Exp Toxiicol Pathol 2002 Jul; 54 (1) 25-9. Or the Journal Calci fied Tissue International vol 72, Number 5, May 2003 537-547 "Von Kossa Staining alone is not sufficient to confirm that mineralization in vitro represents bone formation; the authors of which (Bonewald, Harris, Rosser, Dallas)came out of Texas Health Sciences Center down your way. There are many other such references in a PubMed search. Would that they had published years earlier so I wouldn't have had so many nights and weekends in the lab. But my thesis adviser honestly did not say there would be much glory and stardom, just lots of hard work. Raymond Koelling Phenopath Laboratories Seattle, WA -------------- Original message -------------- From: "Molinari, Betsy" > Hi, > > Has anyone run a Von Kossa on a tissue culture? > > Thanks, > > Betsy > > > > > > Betsy Molinari HT (ASCP) > > Texas Heart Institute > > Cardiovascular Pathology > > 6770 Bertner Ave. > > Houston,TX 77030 > > 832-355-6524 > > 832-355-6812 (fax) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BMolinari <@t> heart.thi.tmc.edu Fri Mar 23 06:29:49 2007 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri Mar 23 06:29:55 2007 Subject: [Histonet] Von Kossa In-Reply-To: Message-ID: Sorry Raymond..(not Ralph) Friday..I have had my Starbucks yet . Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Friday, March 23, 2007 5:42 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Von Kossa Ralph, Thank you so much for your input. Now that I know it has been done and with your advice I feel I can move forward with more confidence. Thanks again. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: koellingr@comcast.net [mailto:koellingr@comcast.net] Sent: Friday, March 23, 2007 12:47 AM To: Molinari, Betsy; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Von Kossa Betsy, In case you haven't had specific procedural reply's, I'll say yes. Did it for several years but back where I can't get at notes and 15 years of memory loss. Did VK on my cultures I maintained for project. Performed on 8-well tissue culture slides, both glass and plastic and tissue culture plates of all sizes. The procedure is basically the same as the VK you know-it just needs a bit of inginuity. Anything plastic obviously you can't go through xylenes but that didn't matter as after NFR, washed in water, got a coverlip on somehow with aqueous media and photographed right on plate with inverted scope or slide as usual. Didn't use "the sun" but a great lamp. Make sure you have a good positive control to work up procedure. I made up my own pos and neg tissue culture controls for VK as my experiments went forward. Look in PubMed and find van Griensven M, Exp Toxiicol Pathol 2002 Jul; 54 (1) 25-9. Or the Journal Calci fied Tissue International vol 72, Number 5, May 2003 537-547 "Von Kossa Staining alone is not sufficient to confirm that mineralization in vitro represents bone formation; the authors of which (Bonewald, Harris, Rosser, Dallas)came out of Texas Health Sciences Center down your way. There are many other such references in a PubMed search. Would that they had published years earlier so I wouldn't have had so many nights and weekends in the lab. But my thesis adviser honestly did not say there would be much glory and stardom, just lots of hard work. Raymond Koelling Phenopath Laboratories Seattle, WA -------------- Original message -------------- From: "Molinari, Betsy" > Hi, > > Has anyone run a Von Kossa on a tissue culture? > > Thanks, > > Betsy > > > > > > Betsy Molinari HT (ASCP) > > Texas Heart Institute > > Cardiovascular Pathology > > 6770 Bertner Ave. > > Houston,TX 77030 > > 832-355-6524 > > 832-355-6812 (fax) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histology <@t> gradymem.org Fri Mar 23 08:35:45 2007 From: histology <@t> gradymem.org (histology@gradymem.org) Date: Fri Mar 23 08:35:54 2007 Subject: AW: [Histonet] delivery time of last tray of H&E In-Reply-To: <000001c76cb3$1e9d2770$6412a8c0@dielangs.at> References: <407F05A128805F4C879A33DBA32E618E20C2A3@TRFT-EX01.xRothGen.nhs.uk> <000001c76cb3$1e9d2770$6412a8c0@dielangs.at> Message-ID: We are a rural hospital path lab. We used to try to get the biopsies out by 8:30 but the pathologist usually drags in around 10:30am now, so we don't worry much about the time anymore. Angie Barnett, HTL(ASCP) Grady Memorial Hospital Pathology Department 405/224-2258 histology@gradymem.org ----- Original Message ----- From: Gudrun Lang Date: Thursday, March 22, 2007 2:00 pm Subject: AW: [Histonet] delivery time of last tray of H&E To: histonet@lists.utsouthwestern.edu > Lucky Austrian histotechs. The earliest beginning is 5am I think. > My lab is medium in range with 150 to 250 blocks a day. One > histotech begins > at 6.30 with embedding. We deliver our first slides at 9.30. The > last HE is > usually delivered before noon. There are three pathologists, who > look at the > slides until afternoon. And we do the recuts and preperations for IHC > parallel. > Grossing is from 11am until 3pm, with breaks. My personel shift is > from 7.30 > to 3.30. > > Gudrun Lang > > Biomed. Analytikerin > Histolabor > Akh Linz > Krankenhausstr. 9 > 4020 Linz > +43(0)732/7806-6754 > > > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von > MarshallTerry Dr,Consultant Histopathologist > Gesendet: Donnerstag, 22. M?rz 2007 18:28 > An: Akemi Allison-Tacha; Rene J Buesa; Galiotto, Laura; > histonet@lists.utsouthwestern.edu > Betreff: RE: [Histonet] delivery time of last tray of H&E > > This is all bloody unbelievable. Don't any of you sleep? > What is the point of working at night? > > Terry > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi > Allison-Tacha > Sent: 22 March 2007 17:25 > To: Rene J Buesa; Galiotto, Laura; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] delivery time of last tray of H&E > > Ren?--That was similar to the protocol I followed in the old days > before24/6 . We provided rush's 1st, followed by bx's by 8AM, we > did however, > provide special stains and recuts and IHC's by 2-3 PM, depending > if it > wasn't a lengthy procedure. > > If you have off site facilities to provide for, that is a different > situation. You have to work around courier sheduals for > delivering to off > site facilities. Everyone needs their slides and time is money!! > > With the new microwave processing technology that is now in use, > there has > been a shift to having the majority of staff starting at 10 PM at > night.MUCH TO THE DISLIKING OF HISTOTECH"S. > > The day shift is minimum. IHC is done during the day shift, after the > pathologist has determined to do so. > In most situations, a battery of IHC's tests are pre-set, as well > as with > special stains. > > Akemi Allison-Tacha BS, HT (ASCP) HTL > President > Phoenix Lab Consulting & Staffing > Specializing in Histology, SS, IHC, & Microarray Madison, WI > Tele: (925) 788-0900 > E-Mail: akemiat3377@yahoo.com > > --- Rene J Buesa wrote: > > > Laura: > > For a laboratory I used to manage with quite similar annual > workload > > we had the following > > schedule: > > 1- last tissue accepted at 7:00 PM; > > 2- two VIPs atarting with delay to be finished at 4:00 AM next > day;> 3- Rushes ready for the pathologists at 8:00 AM next day; > > 4-All slides ready, the latest, at 1:00 PM. > > 5- All IHC ready by 2 PM > > 6- all HC ready by noon. > > Ren? J. > > > > "Galiotto, Laura" wrote: > > Hello Fellow Histo Tech's, > > Will anyone be willing to share the delivery time of the last > tray of > > H&E for the days workload to the pathologist reading them? > > What is your cutoff time for specimen acceptance? > > What is your annual case workload? > > I am trying to improve the TAT from specimen gross to the H&E > > delivered to the pathologist. Unless we change the cutoff time I > can > > not see how I can do this? Any suggestions? > > We process 22,000 cases a year an average of 280 blks a day > consisting > > of types of tissue. > > Please Help > > Laura > > > > > > ******************* PLEASE NOTE ******************* This > > E-Mail/telefax message and any documents accompanying this > > transmission may contain information that is privileged, > confidential, > > and/or exempt from disclosure under applicable law and is > intended > > solely for the addressee(s) named above. If you are not the > intended > > addressee/recipient, you are hereby notified that any use of, > > disclosure, copying, distribution, or reliance on the contents > of this > > E-Mail/telefax information is strictly prohibited and may result > in > > legal action against you. Please reply to the sender advising of > the > > error in transmission and immediately delete/destroy the message > and > > any accompanying documents. Thank you. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > > > --------------------------------- > > Sucker-punch spam with award-winning protection. > > Try the free Yahoo! Mail Beta. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From CIngles <@t> uwhealth.org Fri Mar 23 08:37:44 2007 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Fri Mar 23 08:40:32 2007 Subject: [Histonet] Looking to relocate References: <616543.91894.qm@web62405.mail.re1.yahoo.com> Message-ID: <08A0A863637F1349BBFD83A96B27A50A120011@uwhis-xchng3.uwhis.hosp.wisc.edu> I'm looking to relocate to the Bahamas... Oh sorry, it's Friday and I worked a 12 yesterday. :) At least most of the snow is gone and it's going to be 60 today! Oh yea, I have to work in a windowless lab... Claire Ingles UW Hospital and Clinics Madison WI ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Dan Malzahn Sent: Thu 3/22/2007 10:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Looking to relocate to Youngstown, Oh area Hello All, Just wondering if anyone knows of any jobs that might be in the Youngstown, OH area. I am looking to relocate to that area and I am having a hard time finding any Histology jobs in that area. Any help would be greatly appreciated. Thankyou From cmiller <@t> physlab.com Fri Mar 23 08:43:41 2007 From: cmiller <@t> physlab.com (Cheri Miller) Date: Fri Mar 23 08:43:54 2007 Subject: [Histonet] delivery time of last tray of H&E In-Reply-To: <407F05A128805F4C879A33DBA32E618E20C2A3@TRFT-EX01.xRothGen.nhs.uk> References: <407F05A128805F4C879A33DBA32E618E20C2A3@TRFT-EX01.xRothGen.nhs.uk> Message-ID: <002601c76d51$46cd0b90$db01a8c0@plab.local> Turn around time Terry. For us it's about getting the slides to the Pathologist early so they may order whatever additional testing needed and still get a hard copy report to the Clinician by the end of the day. We have 24 hour turnaround for most cases. Cheri Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Thursday, March 22, 2007 12:28 PM To: Akemi Allison-Tacha; Rene J Buesa; Galiotto, Laura; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] delivery time of last tray of H&E This is all bloody unbelievable. Don't any of you sleep? What is the point of working at night? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: 22 March 2007 17:25 To: Rene J Buesa; Galiotto, Laura; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] delivery time of last tray of H&E Ren?--That was similar to the protocol I followed in the old days before 24/6 . We provided rush's 1st, followed by bx's by 8AM, we did however, provide special stains and recuts and IHC's by 2-3 PM, depending if it wasn't a lengthy procedure. If you have off site facilities to provide for, that is a different situation. You have to work around courier sheduals for delivering to off site facilities. Everyone needs their slides and time is money!! With the new microwave processing technology that is now in use, there has been a shift to having the majority of staff starting at 10 PM at night. MUCH TO THE DISLIKING OF HISTOTECH"S. The day shift is minimum. IHC is done during the day shift, after the pathologist has determined to do so. In most situations, a battery of IHC's tests are pre-set, as well as with special stains. Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting & Staffing Specializing in Histology, SS, IHC, & Microarray Madison, WI Tele: (925) 788-0900 E-Mail: akemiat3377@yahoo.com --- Rene J Buesa wrote: > Laura: > For a laboratory I used to manage with quite similar annual workload > we had the following > schedule: > 1- last tissue accepted at 7:00 PM; > 2- two VIPs atarting with delay to be finished at 4:00 AM next day; > 3- Rushes ready for the pathologists at 8:00 AM next day; > 4-All slides ready, the latest, at 1:00 PM. > 5- All IHC ready by 2 PM > 6- all HC ready by noon. > Ren? J. > > "Galiotto, Laura" wrote: > Hello Fellow Histo Tech's, > Will anyone be willing to share the delivery time of the last tray of > H&E for the days workload to the pathologist reading them? > What is your cutoff time for specimen acceptance? > What is your annual case workload? > I am trying to improve the TAT from specimen gross to the H&E > delivered to the pathologist. Unless we change the cutoff time I can > not see how I can do this? Any suggestions? > We process 22,000 cases a year an average of 280 blks a day consisting > of types of tissue. > Please Help > Laura > > > ******************* PLEASE NOTE ******************* This > E-Mail/telefax message and any documents accompanying this > transmission may contain information that is privileged, confidential, > and/or exempt from disclosure under applicable law and is intended > solely for the addressee(s) named above. If you are not the intended > addressee/recipient, you are hereby notified that any use of, > disclosure, copying, distribution, or reliance on the contents of this > E-Mail/telefax information is strictly prohibited and may result in > legal action against you. Please reply to the sender advising of the > error in transmission and immediately delete/destroy the message and > any accompanying documents. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Sucker-punch spam with award-winning protection. > Try the free Yahoo! Mail Beta. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nancy.troiano <@t> yale.edu Fri Mar 23 08:51:56 2007 From: nancy.troiano <@t> yale.edu (Nancy W. Troiano) Date: Fri Mar 23 08:52:06 2007 Subject: [Histonet] Von Kossa on tissue culture Message-ID: <5.2.1.1.2.20070323085122.02819fd0@email.med.yale.edu> Hi - we do VK on tissue cultures routinely - send me your fax number and I will fax our protocol to you. From gu.lang <@t> gmx.at Fri Mar 23 10:43:54 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Mar 23 10:44:20 2007 Subject: AW: [Histonet] delivery time of last tray of H&E In-Reply-To: <002601c76d51$46cd0b90$db01a8c0@plab.local> Message-ID: <000301c76d62$19503d20$6412a8c0@dielangs.at> And then ... the patient has to get an appointment for the talk about the report with his/her doctor or has to get a date for the necessary surgery. In most cases there is no reason for such short TATs. Why all this hurry? The specimen aren't properly fixed, people have to work in nightshifts; There is no tumor, that grows in such a speed ... My point of view. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Cheri Miller Gesendet: Freitag, 23. M?rz 2007 14:44 An: 'Marshall Terry Dr,Consultant Histopathologist'; 'Akemi Allison-Tacha'; 'Rene J Buesa'; 'Galiotto, Laura'; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] delivery time of last tray of H&E Turn around time Terry. For us it's about getting the slides to the Pathologist early so they may order whatever additional testing needed and still get a hard copy report to the Clinician by the end of the day. We have 24 hour turnaround for most cases. Cheri Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Thursday, March 22, 2007 12:28 PM To: Akemi Allison-Tacha; Rene J Buesa; Galiotto, Laura; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] delivery time of last tray of H&E This is all bloody unbelievable. Don't any of you sleep? What is the point of working at night? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: 22 March 2007 17:25 To: Rene J Buesa; Galiotto, Laura; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] delivery time of last tray of H&E Ren?--That was similar to the protocol I followed in the old days before 24/6 . We provided rush's 1st, followed by bx's by 8AM, we did however, provide special stains and recuts and IHC's by 2-3 PM, depending if it wasn't a lengthy procedure. If you have off site facilities to provide for, that is a different situation. You have to work around courier sheduals for delivering to off site facilities. Everyone needs their slides and time is money!! With the new microwave processing technology that is now in use, there has been a shift to having the majority of staff starting at 10 PM at night. MUCH TO THE DISLIKING OF HISTOTECH"S. The day shift is minimum. IHC is done during the day shift, after the pathologist has determined to do so. In most situations, a battery of IHC's tests are pre-set, as well as with special stains. Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting & Staffing Specializing in Histology, SS, IHC, & Microarray Madison, WI Tele: (925) 788-0900 E-Mail: akemiat3377@yahoo.com --- Rene J Buesa wrote: > Laura: > For a laboratory I used to manage with quite similar annual workload > we had the following > schedule: > 1- last tissue accepted at 7:00 PM; > 2- two VIPs atarting with delay to be finished at 4:00 AM next day; > 3- Rushes ready for the pathologists at 8:00 AM next day; > 4-All slides ready, the latest, at 1:00 PM. > 5- All IHC ready by 2 PM > 6- all HC ready by noon. > Ren? J. > > "Galiotto, Laura" wrote: > Hello Fellow Histo Tech's, > Will anyone be willing to share the delivery time of the last tray of > H&E for the days workload to the pathologist reading them? > What is your cutoff time for specimen acceptance? > What is your annual case workload? > I am trying to improve the TAT from specimen gross to the H&E > delivered to the pathologist. Unless we change the cutoff time I can > not see how I can do this? Any suggestions? > We process 22,000 cases a year an average of 280 blks a day consisting > of types of tissue. > Please Help > Laura > > > ******************* PLEASE NOTE ******************* This > E-Mail/telefax message and any documents accompanying this > transmission may contain information that is privileged, confidential, > and/or exempt from disclosure under applicable law and is intended > solely for the addressee(s) named above. If you are not the intended > addressee/recipient, you are hereby notified that any use of, > disclosure, copying, distribution, or reliance on the contents of this > E-Mail/telefax information is strictly prohibited and may result in > legal action against you. Please reply to the sender advising of the > error in transmission and immediately delete/destroy the message and > any accompanying documents. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Sucker-punch spam with award-winning protection. > Try the free Yahoo! Mail Beta. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JHAPPEL <@t> PARTNERS.ORG Fri Mar 23 10:49:23 2007 From: JHAPPEL <@t> PARTNERS.ORG (Happel, James F.) Date: Fri Mar 23 10:49:34 2007 Subject: [Histonet] Question about historical photographs Message-ID: Good Day, I am putting together a presentation on the past, present and future of histology/pathology and am looking for historical pictures, drawings, advertisements, etc. to include in the presentation. Does anyone have images I could use or know where I can find some? Thank you. James Happel Massachusetts General Hospital The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From Terry.Marshall <@t> rothgen.nhs.uk Fri Mar 23 10:53:54 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Mar 23 10:54:07 2007 Subject: [Histonet] delivery time of last tray of H&E Message-ID: <407F05A128805F4C879A33DBA32E618E20C2B0@TRFT-EX01.xRothGen.nhs.uk> Absolutely. This comes about when the lab is looking after the interests of the Doctors, not the patients. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: 23 March 2007 15:44 To: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] delivery time of last tray of H&E And then ... the patient has to get an appointment for the talk about the report with his/her doctor or has to get a date for the necessary surgery. In most cases there is no reason for such short TATs. Why all this hurry? The specimen aren't properly fixed, people have to work in nightshifts; There is no tumor, that grows in such a speed ... My point of view. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Cheri Miller Gesendet: Freitag, 23. M?rz 2007 14:44 An: 'Marshall Terry Dr,Consultant Histopathologist'; 'Akemi Allison-Tacha'; 'Rene J Buesa'; 'Galiotto, Laura'; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] delivery time of last tray of H&E Turn around time Terry. For us it's about getting the slides to the Pathologist early so they may order whatever additional testing needed and still get a hard copy report to the Clinician by the end of the day. We have 24 hour turnaround for most cases. Cheri Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Thursday, March 22, 2007 12:28 PM To: Akemi Allison-Tacha; Rene J Buesa; Galiotto, Laura; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] delivery time of last tray of H&E This is all bloody unbelievable. Don't any of you sleep? What is the point of working at night? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: 22 March 2007 17:25 To: Rene J Buesa; Galiotto, Laura; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] delivery time of last tray of H&E Ren?--That was similar to the protocol I followed in the old days before 24/6 . We provided rush's 1st, followed by bx's by 8AM, we did however, provide special stains and recuts and IHC's by 2-3 PM, depending if it wasn't a lengthy procedure. If you have off site facilities to provide for, that is a different situation. You have to work around courier sheduals for delivering to off site facilities. Everyone needs their slides and time is money!! With the new microwave processing technology that is now in use, there has been a shift to having the majority of staff starting at 10 PM at night. MUCH TO THE DISLIKING OF HISTOTECH"S. The day shift is minimum. IHC is done during the day shift, after the pathologist has determined to do so. In most situations, a battery of IHC's tests are pre-set, as well as with special stains. Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting & Staffing Specializing in Histology, SS, IHC, & Microarray Madison, WI Tele: (925) 788-0900 E-Mail: akemiat3377@yahoo.com --- Rene J Buesa wrote: > Laura: > For a laboratory I used to manage with quite similar annual workload > we had the following > schedule: > 1- last tissue accepted at 7:00 PM; > 2- two VIPs atarting with delay to be finished at 4:00 AM next day; > 3- Rushes ready for the pathologists at 8:00 AM next day; > 4-All slides ready, the latest, at 1:00 PM. > 5- All IHC ready by 2 PM > 6- all HC ready by noon. > Ren? J. > > "Galiotto, Laura" wrote: > Hello Fellow Histo Tech's, > Will anyone be willing to share the delivery time of the last tray of > H&E for the days workload to the pathologist reading them? > What is your cutoff time for specimen acceptance? > What is your annual case workload? > I am trying to improve the TAT from specimen gross to the H&E > delivered to the pathologist. Unless we change the cutoff time I can > not see how I can do this? Any suggestions? > We process 22,000 cases a year an average of 280 blks a day consisting > of types of tissue. > Please Help > Laura > > > ******************* PLEASE NOTE ******************* This > E-Mail/telefax message and any documents accompanying this > transmission may contain information that is privileged, confidential, > and/or exempt from disclosure under applicable law and is intended > solely for the addressee(s) named above. If you are not the intended > addressee/recipient, you are hereby notified that any use of, > disclosure, copying, distribution, or reliance on the contents of this > E-Mail/telefax information is strictly prohibited and may result in > legal action against you. Please reply to the sender advising of the > error in transmission and immediately delete/destroy the message and > any accompanying documents. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Sucker-punch spam with award-winning protection. > Try the free Yahoo! Mail Beta. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Fri Mar 23 11:38:18 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Mar 23 11:38:37 2007 Subject: [Histonet] Problem with intestinal epithelium In-Reply-To: Message-ID: <003401c76d69$aa75a9a0$6501a8c0@Patsy> I would skip the pbs wash and get them into fixative asap and fix for a minium of 24hrs. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Wednesday, March 21, 2007 10:10 AM To: Mikael Niku Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Problem with intestinal epithelium We get bovine intestine from a local university that has an agriculture program. We provide them with a large container of formalin and ask that they open the intestine and place it immediately in the formalin. We have had great preservation of the epithelium. The piece of intestine is about 8-10 inches long. Jennifer Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu Mikael Niku Sent by: histonet-bounces@lists.utsouthwestern.edu 03/21/2007 03:49 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Problem with intestinal epithelium Dear Histonetters, we are trying to make good-quality sections (paraffin and cryo) of bovine intestines. The problem is that the epithelium seems to be stripped off whatever we do. This seems to be a problem especially with slaughterhouse material, although the delay from death to sampling is less than 30 min. Currently we are cutting the intestine to pieces, washing with PBS buffer, and fixing immediately in buffered paraformaldehyde or freezing in isopentane / liquid nitrogen. Any ideas, anyone? Or are we just too late here, could the mucosa be destroyed so soon after death? With best regards, Mikael Niku -- //////////////////////////////////////////////////////////// Mikael Niku URL: www.helsinki.fi/~mniku/ University of Helsinki Dept. Basic Veterinary Sciences - Mit?k? mielt? olen l?nsimaisesta sivistyksest?? Minusta se olisi erinomainen ajatus! - Gandhi //////////////////////////////////////////////////////////// _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Fri Mar 23 11:45:34 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Mar 23 11:45:53 2007 Subject: [Histonet] Problem with intestinal epithelium In-Reply-To: <003401c76d69$aa75a9a0$6501a8c0@Patsy> Message-ID: <003c01c76d6a$adacc350$6501a8c0@Patsy> Oh yea, and certainly open the intestine up so the epithelium mucosa is readily exposed to the fixative. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Friday, March 23, 2007 10:38 AM To: 'Histonet' Subject: RE: [Histonet] Problem with intestinal epithelium I would skip the pbs wash and get them into fixative asap and fix for a minium of 24hrs. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Wednesday, March 21, 2007 10:10 AM To: Mikael Niku Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Problem with intestinal epithelium We get bovine intestine from a local university that has an agriculture program. We provide them with a large container of formalin and ask that they open the intestine and place it immediately in the formalin. We have had great preservation of the epithelium. The piece of intestine is about 8-10 inches long. Jennifer Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu Mikael Niku Sent by: histonet-bounces@lists.utsouthwestern.edu 03/21/2007 03:49 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Problem with intestinal epithelium Dear Histonetters, we are trying to make good-quality sections (paraffin and cryo) of bovine intestines. The problem is that the epithelium seems to be stripped off whatever we do. This seems to be a problem especially with slaughterhouse material, although the delay from death to sampling is less than 30 min. Currently we are cutting the intestine to pieces, washing with PBS buffer, and fixing immediately in buffered paraformaldehyde or freezing in isopentane / liquid nitrogen. Any ideas, anyone? Or are we just too late here, could the mucosa be destroyed so soon after death? With best regards, Mikael Niku -- //////////////////////////////////////////////////////////// Mikael Niku URL: www.helsinki.fi/~mniku/ University of Helsinki Dept. Basic Veterinary Sciences - Mit?k? mielt? olen l?nsimaisesta sivistyksest?? Minusta se olisi erinomainen ajatus! - Gandhi //////////////////////////////////////////////////////////// _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ryaskovich <@t> dir.nidcr.nih.gov Fri Mar 23 11:57:15 2007 From: ryaskovich <@t> dir.nidcr.nih.gov (Yaskovich, Ruth A (NIH/NIDCR) [E]) Date: Fri Mar 23 11:57:27 2007 Subject: [Histonet] Problem with intestinal epithelium In-Reply-To: <003c01c76d6a$adacc350$6501a8c0@Patsy> References: <003401c76d69$aa75a9a0$6501a8c0@Patsy> <003c01c76d6a$adacc350$6501a8c0@Patsy> Message-ID: Make sure you have plenty of volume and change fix after 1st day to fresh. May-be give them a second day of fix. (sometimes tissues are bloody and dirty) Ruth Yaskovich National Institutes of Health National Institute of Dental and Craniofacial Research Neurobiology and Pain Therapeutics Branch -----Original Message----- From: Patsy Ruegg [mailto:pruegg@ihctech.net] Sent: Friday, March 23, 2007 12:46 PM To: 'Patsy Ruegg'; 'Histonet' Subject: RE: [Histonet] Problem with intestinal epithelium Oh yea, and certainly open the intestine up so the epithelium mucosa is readily exposed to the fixative. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Friday, March 23, 2007 10:38 AM To: 'Histonet' Subject: RE: [Histonet] Problem with intestinal epithelium I would skip the pbs wash and get them into fixative asap and fix for a minium of 24hrs. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Wednesday, March 21, 2007 10:10 AM To: Mikael Niku Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Problem with intestinal epithelium We get bovine intestine from a local university that has an agriculture program. We provide them with a large container of formalin and ask that they open the intestine and place it immediately in the formalin. We have had great preservation of the epithelium. The piece of intestine is about 8-10 inches long. Jennifer Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu Mikael Niku Sent by: histonet-bounces@lists.utsouthwestern.edu 03/21/2007 03:49 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Problem with intestinal epithelium Dear Histonetters, we are trying to make good-quality sections (paraffin and cryo) of bovine intestines. The problem is that the epithelium seems to be stripped off whatever we do. This seems to be a problem especially with slaughterhouse material, although the delay from death to sampling is less than 30 min. Currently we are cutting the intestine to pieces, washing with PBS buffer, and fixing immediately in buffered paraformaldehyde or freezing in isopentane / liquid nitrogen. Any ideas, anyone? Or are we just too late here, could the mucosa be destroyed so soon after death? With best regards, Mikael Niku -- //////////////////////////////////////////////////////////// Mikael Niku URL: www.helsinki.fi/~mniku/ University of Helsinki Dept. Basic Veterinary Sciences - Mit?k? mielt? olen l?nsimaisesta sivistyksest?? Minusta se olisi erinomainen ajatus! - Gandhi //////////////////////////////////////////////////////////// _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Fri Mar 23 11:57:30 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Mar 23 11:58:36 2007 Subject: AW: [Histonet] Question about historical photographs In-Reply-To: Message-ID: <000001c76d6c$79613de0$6412a8c0@dielangs.at> http://www.unifi.it/unifi/dbag/oldtools/en/microtomes.html http://micro.magnet.fsu.edu/primer/museum/index.html http://caliban.mpiz-koeln.mpg.de/~stueber/frey/index.html perhaps something you can use Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Happel, James F. Gesendet: Freitag, 23. M?rz 2007 16:49 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Question about historical photographs Good Day, I am putting together a presentation on the past, present and future of histology/pathology and am looking for historical pictures, drawings, advertisements, etc. to include in the presentation. Does anyone have images I could use or know where I can find some? Thank you. James Happel Massachusetts General Hospital The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMcCormick <@t> schosp.org Fri Mar 23 12:26:46 2007 From: JMcCormick <@t> schosp.org (McCormick, James) Date: Fri Mar 23 12:27:38 2007 Subject: [Histonet] Question about historical photographs In-Reply-To: <000001c76d6c$79613de0$6412a8c0@dielangs.at> References: <000001c76d6c$79613de0$6412a8c0@dielangs.at> Message-ID: <913FAC2B773C19488E26AE6572180FA50AE80428@exch01.schosp.org> Mr. Happel, We have an extensive library of books and illustrations that concern the history of Histotechnology.....PAST PRESENT FUTURE.... I am an Author of many articles and teach workshops for the NSH on the history of our craft. Contact Mr. Drew Mehta for source images that will be helpful. These come from my Science Heritage Library Collection. email dnmehta@Coretech-holdings.com . I have copied Drew this note. GOOD LUCK, J.B.McCormick,M.D. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Friday, March 23, 2007 10:58 AM To: 'Happel, James F.' Cc: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] Question about historical photographs http://www.unifi.it/unifi/dbag/oldtools/en/microtomes.html http://micro.magnet.fsu.edu/primer/museum/index.html http://caliban.mpiz-koeln.mpg.de/~stueber/frey/index.html perhaps something you can use Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Happel, James F. Gesendet: Freitag, 23. M?rz 2007 16:49 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Question about historical photographs Good Day, I am putting together a presentation on the past, present and future of histology/pathology and am looking for historical pictures, drawings, advertisements, etc. to include in the presentation. Does anyone have images I could use or know where I can find some? Thank you. James Happel Massachusetts General Hospital The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. From yelkaco <@t> ticon.net Fri Mar 23 13:18:00 2007 From: yelkaco <@t> ticon.net (S. Coakley) Date: Fri Mar 23 13:09:50 2007 Subject: [Histonet] Part time Wisconsin-Illinois Message-ID: <000a01c76d77$98308180$98a05f45@yelkaco> Good afternoon, I'm looking for a HT position, perferably PT or as needed in the Janesville/Madison, WI or Rockford Ill area. I have over 14 years as an HT(ASCP). Thanks From b-frederick <@t> northwestern.edu Fri Mar 23 13:13:35 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Mar 23 13:13:43 2007 Subject: [Histonet] IHC orders Message-ID: <000501c76d76$f969fdb0$d00f7ca5@lurie.northwestern.edu> Geez, I feel privileged. Working I research, both human and animal we have the luxury of only having to give quarterly reports to our coordinating center for clinical trials. Of course, half the time we are running around at the last minute as we were not told the due dates on a lot of it- our IHC is ongoing and then gets scored and scanned into ACISII for review. Most studies we do have a minimum of 4 stains plus "central review" If any of you ae familiar with ECOG you know from whence I speak!!! Bernice Bernice Frederick Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 From BMolinari <@t> heart.thi.tmc.edu Fri Mar 23 13:14:34 2007 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri Mar 23 13:14:38 2007 Subject: [Histonet] Von Kossa In-Reply-To: Message-ID: Thank you Lesley. I now have several options to work with. Have a good weekend. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: Lesley Weston [mailto:leswes@shaw.ca] Sent: Friday, March 23, 2007 11:38 AM To: Molinari, Betsy Subject: Re: [Histonet] Von Kossa Yes, some time ago. After fixing the cells in their dishes, I did the rest of the staining as usual by pouring the reagents in and out of the dishes, and then counterstained very lightly with toluidine blue. It worked very well. -- Lesley Weston > From: "Molinari, Betsy" > Date: Thu, 22 Mar 2007 14:47:33 -0500 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Von Kossa > > Hi, > > Has anyone run a Von Kossa on a tissue culture? > > Thanks, > > Betsy > > > > > > Betsy Molinari HT (ASCP) > > Texas Heart Institute > > Cardiovascular Pathology > > 6770 Bertner Ave. > > Houston,TX 77030 > > 832-355-6524 > > 832-355-6812 (fax) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dlcowie <@t> prodigy.net Fri Mar 23 14:58:33 2007 From: dlcowie <@t> prodigy.net (Dawn Cowie) Date: Fri Mar 23 14:58:41 2007 Subject: [Histonet] delivery time of last tray of H&E In-Reply-To: <407F05A128805F4C879A33DBA32E618E20C2B0@TRFT-EX01.xRothGen.nhs.uk> Message-ID: <244430.48477.qm@web81007.mail.mud.yahoo.com> You're right. And don't forget, it's business. Clients (clinicians) are promised faster and faster turnaround time and you'd better deliver, or they'll send their specimens to another lab. I don't mean to sound so cynical, but its true. The thing is though, I don't think the clinician necessarily gets back to the patient in such short time with the findings, so what is the point? Gudrun is also right in saying that the specimens aren't properly fixed, you have to go back and reprocess, etc. Now it's taken longer from start to finish than it would have done had it been allowed to fix adequately in the first place. The beat goes on....... Dawn "Marshall Terry Dr, Consultant Histopathologist" wrote: Absolutely. This comes about when the lab is looking after the interests of the Doctors, not the patients. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: 23 March 2007 15:44 To: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] delivery time of last tray of H&E And then ... the patient has to get an appointment for the talk about the report with his/her doctor or has to get a date for the necessary surgery. In most cases there is no reason for such short TATs. Why all this hurry? The specimen aren't properly fixed, people have to work in nightshifts; There is no tumor, that grows in such a speed ... My point of view. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Cheri Miller Gesendet: Freitag, 23. M?rz 2007 14:44 An: 'Marshall Terry Dr,Consultant Histopathologist'; 'Akemi Allison-Tacha'; 'Rene J Buesa'; 'Galiotto, Laura'; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] delivery time of last tray of H&E Turn around time Terry. For us it's about getting the slides to the Pathologist early so they may order whatever additional testing needed and still get a hard copy report to the Clinician by the end of the day. We have 24 hour turnaround for most cases. Cheri Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Thursday, March 22, 2007 12:28 PM To: Akemi Allison-Tacha; Rene J Buesa; Galiotto, Laura; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] delivery time of last tray of H&E This is all bloody unbelievable. Don't any of you sleep? What is the point of working at night? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: 22 March 2007 17:25 To: Rene J Buesa; Galiotto, Laura; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] delivery time of last tray of H&E Ren?--That was similar to the protocol I followed in the old days before 24/6 . We provided rush's 1st, followed by bx's by 8AM, we did however, provide special stains and recuts and IHC's by 2-3 PM, depending if it wasn't a lengthy procedure. If you have off site facilities to provide for, that is a different situation. You have to work around courier sheduals for delivering to off site facilities. Everyone needs their slides and time is money!! With the new microwave processing technology that is now in use, there has been a shift to having the majority of staff starting at 10 PM at night. MUCH TO THE DISLIKING OF HISTOTECH"S. The day shift is minimum. IHC is done during the day shift, after the pathologist has determined to do so. In most situations, a battery of IHC's tests are pre-set, as well as with special stains. Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting & Staffing Specializing in Histology, SS, IHC, & Microarray Madison, WI Tele: (925) 788-0900 E-Mail: akemiat3377@yahoo.com --- Rene J Buesa wrote: > Laura: > For a laboratory I used to manage with quite similar annual workload > we had the following > schedule: > 1- last tissue accepted at 7:00 PM; > 2- two VIPs atarting with delay to be finished at 4:00 AM next day; > 3- Rushes ready for the pathologists at 8:00 AM next day; > 4-All slides ready, the latest, at 1:00 PM. > 5- All IHC ready by 2 PM > 6- all HC ready by noon. > Ren? J. > > "Galiotto, Laura" wrote: > Hello Fellow Histo Tech's, > Will anyone be willing to share the delivery time of the last tray of > H&E for the days workload to the pathologist reading them? > What is your cutoff time for specimen acceptance? > What is your annual case workload? > I am trying to improve the TAT from specimen gross to the H&E > delivered to the pathologist. Unless we change the cutoff time I can > not see how I can do this? Any suggestions? > We process 22,000 cases a year an average of 280 blks a day consisting > of types of tissue. > Please Help > Laura > > > ******************* PLEASE NOTE ******************* This > E-Mail/telefax message and any documents accompanying this > transmission may contain information that is privileged, confidential, > and/or exempt from disclosure under applicable law and is intended > solely for the addressee(s) named above. If you are not the intended > addressee/recipient, you are hereby notified that any use of, > disclosure, copying, distribution, or reliance on the contents of this > E-Mail/telefax information is strictly prohibited and may result in > legal action against you. Please reply to the sender advising of the > error in transmission and immediately delete/destroy the message and > any accompanying documents. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Sucker-punch spam with award-winning protection. > Try the free Yahoo! Mail Beta. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Fri Mar 23 15:01:31 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Mar 23 15:02:00 2007 Subject: [Histonet] delivery time of last tray of H&E In-Reply-To: <244430.48477.qm@web81007.mail.mud.yahoo.com> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D38F2@sjhaexc02.sjha.org> Also, throw in the billing factor. We have 3 days to get the charges in before they are considered late...sometimes we're late before we begin.... The beat goes on some more... Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Dawn Cowie Sent: Friday, March 23, 2007 3:59 PM To: Marshall Terry Dr,Consultant Histopathologist; gu.lang@gmx.at; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] delivery time of last tray of H&E You're right. And don't forget, it's business. Clients (clinicians) are promised faster and faster turnaround time and you'd better deliver, or they'll send their specimens to another lab. I don't mean to sound so cynical, but its true. The thing is though, I don't think the clinician necessarily gets back to the patient in such short time with the findings, so what is the point? Gudrun is also right in saying that the specimens aren't properly fixed, you have to go back and reprocess, etc. Now it's taken longer from start to finish than it would have done had it been allowed to fix adequately in the first place. The beat goes on....... Dawn "Marshall Terry Dr, Consultant Histopathologist" wrote: Absolutely. This comes about when the lab is looking after the interests of the Doctors, not the patients. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: 23 March 2007 15:44 To: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] delivery time of last tray of H&E And then ... the patient has to get an appointment for the talk about the report with his/her doctor or has to get a date for the necessary surgery. In most cases there is no reason for such short TATs. Why all this hurry? The specimen aren't properly fixed, people have to work in nightshifts; There is no tumor, that grows in such a speed ... My point of view. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Cheri Miller Gesendet: Freitag, 23. M?rz 2007 14:44 An: 'Marshall Terry Dr,Consultant Histopathologist'; 'Akemi Allison-Tacha'; 'Rene J Buesa'; 'Galiotto, Laura'; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] delivery time of last tray of H&E Turn around time Terry. For us it's about getting the slides to the Pathologist early so they may order whatever additional testing needed and still get a hard copy report to the Clinician by the end of the day. We have 24 hour turnaround for most cases. Cheri Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Thursday, March 22, 2007 12:28 PM To: Akemi Allison-Tacha; Rene J Buesa; Galiotto, Laura; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] delivery time of last tray of H&E This is all bloody unbelievable. Don't any of you sleep? What is the point of working at night? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: 22 March 2007 17:25 To: Rene J Buesa; Galiotto, Laura; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] delivery time of last tray of H&E Ren?--That was similar to the protocol I followed in the old days before 24/6 . We provided rush's 1st, followed by bx's by 8AM, we did however, provide special stains and recuts and IHC's by 2-3 PM, depending if it wasn't a lengthy procedure. If you have off site facilities to provide for, that is a different situation. You have to work around courier sheduals for delivering to off site facilities. Everyone needs their slides and time is money!! With the new microwave processing technology that is now in use, there has been a shift to having the majority of staff starting at 10 PM at night. MUCH TO THE DISLIKING OF HISTOTECH"S. The day shift is minimum. IHC is done during the day shift, after the pathologist has determined to do so. In most situations, a battery of IHC's tests are pre-set, as well as with special stains. Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting & Staffing Specializing in Histology, SS, IHC, & Microarray Madison, WI Tele: (925) 788-0900 E-Mail: akemiat3377@yahoo.com --- Rene J Buesa wrote: > Laura: > For a laboratory I used to manage with quite similar annual workload > we had the following > schedule: > 1- last tissue accepted at 7:00 PM; > 2- two VIPs atarting with delay to be finished at 4:00 AM next day; > 3- Rushes ready for the pathologists at 8:00 AM next day; > 4-All slides ready, the latest, at 1:00 PM. > 5- All IHC ready by 2 PM > 6- all HC ready by noon. > Ren? J. > > "Galiotto, Laura" wrote: > Hello Fellow Histo Tech's, > Will anyone be willing to share the delivery time of the last tray of > H&E for the days workload to the pathologist reading them? > What is your cutoff time for specimen acceptance? > What is your annual case workload? > I am trying to improve the TAT from specimen gross to the H&E > delivered to the pathologist. Unless we change the cutoff time I can > not see how I can do this? Any suggestions? > We process 22,000 cases a year an average of 280 blks a day consisting > of types of tissue. > Please Help > Laura > > > ******************* PLEASE NOTE ******************* This > E-Mail/telefax message and any documents accompanying this > transmission may contain information that is privileged, confidential, > and/or exempt from disclosure under applicable law and is intended > solely for the addressee(s) named above. If you are not the intended > addressee/recipient, you are hereby notified that any use of, > disclosure, copying, distribution, or reliance on the contents of this > E-Mail/telefax information is strictly prohibited and may result in > legal action against you. Please reply to the sender advising of the > error in transmission and immediately delete/destroy the message and > any accompanying documents. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Sucker-punch spam with award-winning protection. > Try the free Yahoo! Mail Beta. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From JPCOLEMA <@t> sentara.com Fri Mar 23 14:58:42 2007 From: JPCOLEMA <@t> sentara.com (JOHN P COLEMAN) Date: Fri Mar 23 15:19:22 2007 Subject: [Histonet] last tray etc. In-Reply-To: <200703231805.l2NI53V06099@sdclin01.sdc.sentara.com> References: <200703231805.l2NI53V06099@sdclin01.sdc.sentara.com> Message-ID: First of all, insomnia is an HT requirement and personality trait. Second here goes nothing: 7 hospital system, 2 processing/cutting hubs. 18,000/annum cases north hub, 62,000/annum cases south hub, average 280 blocks per 100 cases, 800/annum bone marrow cases 2.5 blocks per case. 100% microwave processing, 2 Sakura units at each hub. South hub-1st run up to 60 cases grossed 8a-9a, cut 10a-12A, 100% grossing by 3 registered PA's from 8Am to 4:30 PM, slides on 180 -220 blocks to one hospital cut 10 p to 3A delivered by 7:00 AM, biopsy blocks, 30-40 cases sent to second site by 7 am, 250-500 main blocks daily to South hub building docs by 10 am, IHC's ordered by 10 AM out by 4 PM avg 400 /day serving all hospitals from 1 central location, North hub- 100% of 250 block load ready and delivered onsite and to second north site by 7:30 AM. Staffing south hub- 3 night HTs, 4 AM hts, 3 evening HTs 3 IHC HT's Staffing North hub- 2 day HT's, 1 ft night HT, 1 pt nite HT. As stated by somebody else, majority is cut by evening/nights,dayshift handles same day processing plus specials recuts, overflow, etc. >>> 3/23/2007 2:05 PM >>> Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Looking to relocate (Ingles Claire) 2. RE: delivery time of last tray of H&E (Cheri Miller) 3. Von Kossa on tissue culture (Nancy W. Troiano) 4. AW: [Histonet] delivery time of last tray of H&E (Gudrun Lang) 5. Question about historical photographs (Happel, James F.) 6. RE: delivery time of last tray of H&E (Marshall Terry Dr, Consultant Histopathologist) 7. RE: Problem with intestinal epithelium (Patsy Ruegg) 8. RE: Problem with intestinal epithelium (Patsy Ruegg) 9. RE: Problem with intestinal epithelium (Yaskovich, Ruth A (NIH/NIDCR) [E]) 10. AW: [Histonet] Question about historical photographs (Gudrun Lang) 11. RE: Question about historical photographs (McCormick, James) ---------------------------------------------------------------------- Message: 1 Date: Fri, 23 Mar 2007 08:37:44 -0500 From: "Ingles Claire" Subject: RE: [Histonet] Looking to relocate To: Message-ID: <08A0A863637F1349BBFD83A96B27A50A120011@uwhis-xchng3.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="iso-8859-1" I'm looking to relocate to the Bahamas... Oh sorry, it's Friday and I worked a 12 yesterday. :) At least most of the snow is gone and it's going to be 60 today! Oh yea, I have to work in a windowless lab... Claire Ingles UW Hospital and Clinics Madison WI ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Dan Malzahn Sent: Thu 3/22/2007 10:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Looking to relocate to Youngstown, Oh area Hello All, Just wondering if anyone knows of any jobs that might be in the Youngstown, OH area. I am looking to relocate to that area and I am having a hard time finding any Histology jobs in that area. Any help would be greatly appreciated. Thankyou ------------------------------ Message: 2 Date: Fri, 23 Mar 2007 08:43:41 -0500 From: "Cheri Miller" Subject: RE: [Histonet] delivery time of last tray of H&E To: "'Marshall Terry Dr, Consultant Histopathologist'" , "'Akemi Allison-Tacha'" , "'Rene J Buesa'" , "'Galiotto, Laura'" , Message-ID: <002601c76d51$46cd0b90$db01a8c0@plab.local> Content-Type: text/plain; charset="iso-8859-1" Turn around time Terry. For us it's about getting the slides to the Pathologist early so they may order whatever additional testing needed and still get a hard copy report to the Clinician by the end of the day. We have 24 hour turnaround for most cases. Cheri Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Thursday, March 22, 2007 12:28 PM To: Akemi Allison-Tacha; Rene J Buesa; Galiotto, Laura; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] delivery time of last tray of H&E This is all bloody unbelievable. Don't any of you sleep? What is the point of working at night? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: 22 March 2007 17:25 To: Rene J Buesa; Galiotto, Laura; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] delivery time of last tray of H&E Ren?--That was similar to the protocol I followed in the old days before 24/6 . We provided rush's 1st, followed by bx's by 8AM, we did however, provide special stains and recuts and IHC's by 2-3 PM, depending if it wasn't a lengthy procedure. If you have off site facilities to provide for, that is a different situation. You have to work around courier sheduals for delivering to off site facilities. Everyone needs their slides and time is money!! With the new microwave processing technology that is now in use, there has been a shift to having the majority of staff starting at 10 PM at night. MUCH TO THE DISLIKING OF HISTOTECH"S. The day shift is minimum. IHC is done during the day shift, after the pathologist has determined to do so. In most situations, a battery of IHC's tests are pre-set, as well as with special stains. Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting & Staffing Specializing in Histology, SS, IHC, & Microarray Madison, WI Tele: (925) 788-0900 E-Mail: akemiat3377@yahoo.com --- Rene J Buesa wrote: > Laura: > For a laboratory I used to manage with quite similar annual workload > we had the following > schedule: > 1- last tissue accepted at 7:00 PM; > 2- two VIPs atarting with delay to be finished at 4:00 AM next day; > 3- Rushes ready for the pathologists at 8:00 AM next day; > 4-All slides ready, the latest, at 1:00 PM. > 5- All IHC ready by 2 PM > 6- all HC ready by noon. > Ren? J. > > "Galiotto, Laura" wrote: > Hello Fellow Histo Tech's, > Will anyone be willing to share the delivery time of the last tray of > H&E for the days workload to the pathologist reading them? > What is your cutoff time for specimen acceptance? > What is your annual case workload? > I am trying to improve the TAT from specimen gross to the H&E > delivered to the pathologist. Unless we change the cutoff time I can > not see how I can do this? Any suggestions? > We process 22,000 cases a year an average of 280 blks a day consisting > of types of tissue. > Please Help > Laura > > > ******************* PLEASE NOTE ******************* This > E-Mail/telefax message and any documents accompanying this > transmission may contain information that is privileged, confidential, > and/or exempt from disclosure under applicable law and is intended > solely for the addressee(s) named above. If you are not the intended > addressee/recipient, you are hereby notified that any use of, > disclosure, copying, distribution, or reliance on the contents of this > E-Mail/telefax information is strictly prohibited and may result in > legal action against you. Please reply to the sender advising of the > error in transmission and immediately delete/destroy the message and > any accompanying documents. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Sucker-punch spam with award-winning protection. > Try the free Yahoo! Mail Beta. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Fri, 23 Mar 2007 08:51:56 -0500 From: "Nancy W. Troiano" Subject: [Histonet] Von Kossa on tissue culture To: histonet@lists.utsouthwestern.edu Message-ID: <5.2.1.1.2.20070323085122.02819fd0@email.med.yale.edu> Content-Type: text/plain; format=flowed; charset=us-ascii Hi - we do VK on tissue cultures routinely - send me your fax number and I will fax our protocol to you. ------------------------------ Message: 4 Date: Fri, 23 Mar 2007 16:43:54 +0100 From: "Gudrun Lang" Subject: AW: [Histonet] delivery time of last tray of H&E To: Message-ID: <000301c76d62$19503d20$6412a8c0@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" And then ... the patient has to get an appointment for the talk about the report with his/her doctor or has to get a date for the necessary surgery. In most cases there is no reason for such short TATs. Why all this hurry? The specimen aren't properly fixed, people have to work in nightshifts; There is no tumor, that grows in such a speed ... My point of view. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Cheri Miller Gesendet: Freitag, 23. M?rz 2007 14:44 An: 'Marshall Terry Dr,Consultant Histopathologist'; 'Akemi Allison-Tacha'; 'Rene J Buesa'; 'Galiotto, Laura'; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] delivery time of last tray of H&E Turn around time Terry. For us it's about getting the slides to the Pathologist early so they may order whatever additional testing needed and still get a hard copy report to the Clinician by the end of the day. We have 24 hour turnaround for most cases. Cheri Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Thursday, March 22, 2007 12:28 PM To: Akemi Allison-Tacha; Rene J Buesa; Galiotto, Laura; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] delivery time of last tray of H&E This is all bloody unbelievable. Don't any of you sleep? What is the point of working at night? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: 22 March 2007 17:25 To: Rene J Buesa; Galiotto, Laura; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] delivery time of last tray of H&E Ren?--That was similar to the protocol I followed in the old days before 24/6 . We provided rush's 1st, followed by bx's by 8AM, we did however, provide special stains and recuts and IHC's by 2-3 PM, depending if it wasn't a lengthy procedure. If you have off site facilities to provide for, that is a different situation. You have to work around courier sheduals for delivering to off site facilities. Everyone needs their slides and time is money!! With the new microwave processing technology that is now in use, there has been a shift to having the majority of staff starting at 10 PM at night. MUCH TO THE DISLIKING OF HISTOTECH"S. The day shift is minimum. IHC is done during the day shift, after the pathologist has determined to do so. In most situations, a battery of IHC's tests are pre-set, as well as with special stains. Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting & Staffing Specializing in Histology, SS, IHC, & Microarray Madison, WI Tele: (925) 788-0900 E-Mail: akemiat3377@yahoo.com --- Rene J Buesa wrote: > Laura: > For a laboratory I used to manage with quite similar annual workload > we had the following > schedule: > 1- last tissue accepted at 7:00 PM; > 2- two VIPs atarting with delay to be finished at 4:00 AM next day; > 3- Rushes ready for the pathologists at 8:00 AM next day; > 4-All slides ready, the latest, at 1:00 PM. > 5- All IHC ready by 2 PM > 6- all HC ready by noon. > Ren? J. > > "Galiotto, Laura" wrote: > Hello Fellow Histo Tech's, > Will anyone be willing to share the delivery time of the last tray of > H&E for the days workload to the pathologist reading them? > What is your cutoff time for specimen acceptance? > What is your annual case workload? > I am trying to improve the TAT from specimen gross to the H&E > delivered to the pathologist. Unless we change the cutoff time I can > not see how I can do this? Any suggestions? > We process 22,000 cases a year an average of 280 blks a day consisting > of types of tissue. > Please Help > Laura > > > ******************* PLEASE NOTE ******************* This > E-Mail/telefax message and any documents accompanying this > transmission may contain information that is privileged, confidential, > and/or exempt from disclosure under applicable law and is intended > solely for the addressee(s) named above. If you are not the intended > addressee/recipient, you are hereby notified that any use of, > disclosure, copying, distribution, or reliance on the contents of this > E-Mail/telefax information is strictly prohibited and may result in > legal action against you. Please reply to the sender advising of the > error in transmission and immediately delete/destroy the message and > any accompanying documents. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Sucker-punch spam with award-winning protection. > Try the free Yahoo! Mail Beta. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Fri, 23 Mar 2007 11:49:23 -0400 From: "Happel, James F." Subject: [Histonet] Question about historical photographs To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Good Day, I am putting together a presentation on the past, present and future of histology/pathology and am looking for historical pictures, drawings, advertisements, etc. to include in the presentation. Does anyone have images I could use or know where I can find some? Thank you. James Happel Massachusetts General Hospital The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. ------------------------------ Message: 6 Date: Fri, 23 Mar 2007 15:53:54 -0000 From: "Marshall Terry Dr, Consultant Histopathologist" Subject: RE: [Histonet] delivery time of last tray of H&E To: , Message-ID: <407F05A128805F4C879A33DBA32E618E20C2B0@TRFT-EX01.xRothGen.nhs.uk> Content-Type: text/plain; charset="iso-8859-1" Absolutely. This comes about when the lab is looking after the interests of the Doctors, not the patients. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: 23 March 2007 15:44 To: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] delivery time of last tray of H&E And then ... the patient has to get an appointment for the talk about the report with his/her doctor or has to get a date for the necessary surgery. In most cases there is no reason for such short TATs. Why all this hurry? The specimen aren't properly fixed, people have to work in nightshifts; There is no tumor, that grows in such a speed ... My point of view. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Cheri Miller Gesendet: Freitag, 23. M?rz 2007 14:44 An: 'Marshall Terry Dr,Consultant Histopathologist'; 'Akemi Allison-Tacha'; 'Rene J Buesa'; 'Galiotto, Laura'; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] delivery time of last tray of H&E Turn around time Terry. For us it's about getting the slides to the Pathologist early so they may order whatever additional testing needed and still get a hard copy report to the Clinician by the end of the day. We have 24 hour turnaround for most cases. Cheri Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Thursday, March 22, 2007 12:28 PM To: Akemi Allison-Tacha; Rene J Buesa; Galiotto, Laura; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] delivery time of last tray of H&E This is all bloody unbelievable. Don't any of you sleep? What is the point of working at night? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: 22 March 2007 17:25 To: Rene J Buesa; Galiotto, Laura; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] delivery time of last tray of H&E Ren?--That was similar to the protocol I followed in the old days before 24/6 . We provided rush's 1st, followed by bx's by 8AM, we did however, provide special stains and recuts and IHC's by 2-3 PM, depending if it wasn't a lengthy procedure. If you have off site facilities to provide for, that is a different situation. You have to work around courier sheduals for delivering to off site facilities. Everyone needs their slides and time is money!! With the new microwave processing technology that is now in use, there has been a shift to having the majority of staff starting at 10 PM at night. MUCH TO THE DISLIKING OF HISTOTECH"S. The day shift is minimum. IHC is done during the day shift, after the pathologist has determined to do so. In most situations, a battery of IHC's tests are pre-set, as well as with special stains. Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting & Staffing Specializing in Histology, SS, IHC, & Microarray Madison, WI Tele: (925) 788-0900 E-Mail: akemiat3377@yahoo.com --- Rene J Buesa wrote: > Laura: > For a laboratory I used to manage with quite similar annual workload > we had the following > schedule: > 1- last tissue accepted at 7:00 PM; > 2- two VIPs atarting with delay to be finished at 4:00 AM next day; > 3- Rushes ready for the pathologists at 8:00 AM next day; > 4-All slides ready, the latest, at 1:00 PM. > 5- All IHC ready by 2 PM > 6- all HC ready by noon. > Ren? J. > > "Galiotto, Laura" wrote: > Hello Fellow Histo Tech's, > Will anyone be willing to share the delivery time of the last tray of > H&E for the days workload to the pathologist reading them? > What is your cutoff time for specimen acceptance? > What is your annual case workload? > I am trying to improve the TAT from specimen gross to the H&E > delivered to the pathologist. Unless we change the cutoff time I can > not see how I can do this? Any suggestions? > We process 22,000 cases a year an average of 280 blks a day consisting > of types of tissue. > Please Help > Laura > > > ******************* PLEASE NOTE ******************* This > E-Mail/telefax message and any documents accompanying this > transmission may contain information that is privileged, confidential, > and/or exempt from disclosure under applicable law and is intended > solely for the addressee(s) named above. If you are not the intended > addressee/recipient, you are hereby notified that any use of, > disclosure, copying, distribution, or reliance on the contents of this > E-Mail/telefax information is strictly prohibited and may result in > legal action against you. Please reply to the sender advising of the > error in transmission and immediately delete/destroy the message and > any accompanying documents. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Sucker-punch spam with award-winning protection. > Try the free Yahoo! Mail Beta. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Fri, 23 Mar 2007 10:38:18 -0600 From: "Patsy Ruegg" Subject: RE: [Histonet] Problem with intestinal epithelium To: "'Histonet'" Message-ID: <003401c76d69$aa75a9a0$6501a8c0@Patsy> Content-Type: text/plain; charset="iso-8859-1" I would skip the pbs wash and get them into fixative asap and fix for a minium of 24hrs. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Wednesday, March 21, 2007 10:10 AM To: Mikael Niku Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Problem with intestinal epithelium We get bovine intestine from a local university that has an agriculture program. We provide them with a large container of formalin and ask that they open the intestine and place it immediately in the formalin. We have had great preservation of the epithelium. The piece of intestine is about 8-10 inches long. Jennifer Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu Mikael Niku Sent by: histonet-bounces@lists.utsouthwestern.edu 03/21/2007 03:49 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Problem with intestinal epithelium Dear Histonetters, we are trying to make good-quality sections (paraffin and cryo) of bovine intestines. The problem is that the epithelium seems to be stripped off whatever we do. This seems to be a problem especially with slaughterhouse material, although the delay from death to sampling is less than 30 min. Currently we are cutting the intestine to pieces, washing with PBS buffer, and fixing immediately in buffered paraformaldehyde or freezing in isopentane / liquid nitrogen. Any ideas, anyone? Or are we just too late here, could the mucosa be destroyed so soon after death? With best regards, Mikael Niku -- //////////////////////////////////////////////////////////// Mikael Niku URL: www.helsinki.fi/~mniku/ University of Helsinki Dept. Basic Veterinary Sciences - Mit?k? mielt? olen l?nsimaisesta sivistyksest?? Minusta se olisi erinomainen ajatus! - Gandhi //////////////////////////////////////////////////////////// _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Fri, 23 Mar 2007 10:45:34 -0600 From: "Patsy Ruegg" Subject: RE: [Histonet] Problem with intestinal epithelium To: "'Patsy Ruegg'" , "'Histonet'" Message-ID: <003c01c76d6a$adacc350$6501a8c0@Patsy> Content-Type: text/plain; charset="iso-8859-1" Oh yea, and certainly open the intestine up so the epithelium mucosa is readily exposed to the fixative. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Friday, March 23, 2007 10:38 AM To: 'Histonet' Subject: RE: [Histonet] Problem with intestinal epithelium I would skip the pbs wash and get them into fixative asap and fix for a minium of 24hrs. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Wednesday, March 21, 2007 10:10 AM To: Mikael Niku Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Problem with intestinal epithelium We get bovine intestine from a local university that has an agriculture program. We provide them with a large container of formalin and ask that they open the intestine and place it immediately in the formalin. We have had great preservation of the epithelium. The piece of intestine is about 8-10 inches long. Jennifer Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu Mikael Niku Sent by: histonet-bounces@lists.utsouthwestern.edu 03/21/2007 03:49 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Problem with intestinal epithelium Dear Histonetters, we are trying to make good-quality sections (paraffin and cryo) of bovine intestines. The problem is that the epithelium seems to be stripped off whatever we do. This seems to be a problem especially with slaughterhouse material, although the delay from death to sampling is less than 30 min. Currently we are cutting the intestine to pieces, washing with PBS buffer, and fixing immediately in buffered paraformaldehyde or freezing in isopentane / liquid nitrogen. Any ideas, anyone? Or are we just too late here, could the mucosa be destroyed so soon after death? With best regards, Mikael Niku -- //////////////////////////////////////////////////////////// Mikael Niku URL: www.helsinki.fi/~mniku/ University of Helsinki Dept. Basic Veterinary Sciences - Mit?k? mielt? olen l?nsimaisesta sivistyksest?? Minusta se olisi erinomainen ajatus! - Gandhi //////////////////////////////////////////////////////////// _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Fri, 23 Mar 2007 12:57:15 -0400 From: "Yaskovich, Ruth A \(NIH/NIDCR\) [E]" Subject: RE: [Histonet] Problem with intestinal epithelium To: "Patsy Ruegg" , "Histonet" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Make sure you have plenty of volume and change fix after 1st day to fresh. May-be give them a second day of fix. (sometimes tissues are bloody and dirty) Ruth Yaskovich National Institutes of Health National Institute of Dental and Craniofacial Research Neurobiology and Pain Therapeutics Branch -----Original Message----- From: Patsy Ruegg [mailto:pruegg@ihctech.net] Sent: Friday, March 23, 2007 12:46 PM To: 'Patsy Ruegg'; 'Histonet' Subject: RE: [Histonet] Problem with intestinal epithelium Oh yea, and certainly open the intestine up so the epithelium mucosa is readily exposed to the fixative. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Friday, March 23, 2007 10:38 AM To: 'Histonet' Subject: RE: [Histonet] Problem with intestinal epithelium I would skip the pbs wash and get them into fixative asap and fix for a minium of 24hrs. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Wednesday, March 21, 2007 10:10 AM To: Mikael Niku Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Problem with intestinal epithelium We get bovine intestine from a local university that has an agriculture program. We provide them with a large container of formalin and ask that they open the intestine and place it immediately in the formalin. We have had great preservation of the epithelium. The piece of intestine is about 8-10 inches long. Jennifer Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu Mikael Niku Sent by: histonet-bounces@lists.utsouthwestern.edu 03/21/2007 03:49 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Problem with intestinal epithelium Dear Histonetters, we are trying to make good-quality sections (paraffin and cryo) of bovine intestines. The problem is that the epithelium seems to be stripped off whatever we do. This seems to be a problem especially with slaughterhouse material, although the delay from death to sampling is less than 30 min. Currently we are cutting the intestine to pieces, washing with PBS buffer, and fixing immediately in buffered paraformaldehyde or freezing in isopentane / liquid nitrogen. Any ideas, anyone? Or are we just too late here, could the mucosa be destroyed so soon after death? With best regards, Mikael Niku -- //////////////////////////////////////////////////////////// Mikael Niku URL: www.helsinki.fi/~mniku/ University of Helsinki Dept. Basic Veterinary Sciences - Mit?k? mielt? olen l?nsimaisesta sivistyksest?? Minusta se olisi erinomainen ajatus! - Gandhi //////////////////////////////////////////////////////////// _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Fri, 23 Mar 2007 17:57:30 +0100 From: "Gudrun Lang" Subject: AW: [Histonet] Question about historical photographs To: "'Happel, James F.'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <000001c76d6c$79613de0$6412a8c0@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" http://www.unifi.it/unifi/dbag/oldtools/en/microtomes.html http://micro.magnet.fsu.edu/primer/museum/index.html http://caliban.mpiz-koeln.mpg.de/~stueber/frey/index.html perhaps something you can use Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Happel, James F. Gesendet: Freitag, 23. M?rz 2007 16:49 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Question about historical photographs Good Day, I am putting together a presentation on the past, present and future of histology/pathology and am looking for historical pictures, drawings, advertisements, etc. to include in the presentation. Does anyone have images I could use or know where I can find some? Thank you. James Happel Massachusetts General Hospital The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Fri, 23 Mar 2007 12:26:46 -0500 From: "McCormick, James" Subject: RE: [Histonet] Question about historical photographs To: , "Happel, James F." Cc: histonet@lists.utsouthwestern.edu Message-ID: <913FAC2B773C19488E26AE6572180FA50AE80428@exch01.schosp.org> Content-Type: text/plain; charset="utf-8" Mr. Happel, We have an extensive library of books and illustrations that concern the history of Histotechnology.....PAST PRESENT FUTURE.... I am an Author of many articles and teach workshops for the NSH on the history of our craft. Contact Mr. Drew Mehta for source images that will be helpful. These come from my Science Heritage Library Collection. email dnmehta@Coretech-holdings.com . I have copied Drew this note. GOOD LUCK, J.B.McCormick,M.D. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Friday, March 23, 2007 10:58 AM To: 'Happel, James F.' Cc: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] Question about historical photographs http://www.unifi.it/unifi/dbag/oldtools/en/microtomes.html http://micro.magnet.fsu.edu/primer/museum/index.html http://caliban.mpiz-koeln.mpg.de/~stueber/frey/index.html perhaps something you can use Gudrun Lang -----Urspr??ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Happel, James F. Gesendet: Freitag, 23. M??rz 2007 16:49 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Question about historical photographs Good Day, I am putting together a presentation on the past, present and future of histology/pathology and am looking for historical pictures, drawings, advertisements, etc. to include in the presentation. Does anyone have images I could use or know where I can find some? Thank you. James Happel Massachusetts General Hospital The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 40, Issue 35 **************************************** John P.J. Coleman HT(ASCP)QIHC Clinical Specialist Anatomic Pathology Sentara Laboratory Services Cell/Voicemail(757)287-6657 pager: (757)456-6695 office:-(757)388-3295 From srwilkes <@t> gmail.com Fri Mar 23 16:11:43 2007 From: srwilkes <@t> gmail.com (Steven Wilkes) Date: Fri Mar 23 16:11:51 2007 Subject: [Histonet] seeking immunohistochemistry position in NYC area Message-ID: <8e5827cf0703231411q5428426cma2671f75ef4f8daa@mail.gmail.com> Greetings I am seeking a IHC position in the NYC area. I have a masters in Biology, and a good amount of IHC and general histology experience, both hands on and teaching. Thank you Steven From katri <@t> cogeco.ca Fri Mar 23 18:15:59 2007 From: katri <@t> cogeco.ca (Katri Tuomala) Date: Fri Mar 23 18:15:58 2007 Subject: [Histonet] delivery time of last tray of H&E References: <000301c76d62$19503d20$6412a8c0@dielangs.at> Message-ID: <005b01c76da1$378f5110$6a9a9618@Katri> I totally agree with you Gudrun, I think too many samples get inadequate fixation and processing compromising the patients' samples. There has been an improvement in our lab in regards to larger specimens. Most of them are now opened up, fixed overnight and processed in an "extended program" in VIP. What a joy to cut them, no more fatty holes to hand in! However many biopsies still don't get adequate fixation, particularly if IHC is requested. Katri Katri Tuomala, Hamilton, Ontario ----- Original Message ----- From: "Gudrun Lang" To: Sent: Friday, March 23, 2007 11:43 AM Subject: AW: [Histonet] delivery time of last tray of H&E And then ... the patient has to get an appointment for the talk about the report with his/her doctor or has to get a date for the necessary surgery. In most cases there is no reason for such short TATs. Why all this hurry? The specimen aren't properly fixed, people have to work in nightshifts; There is no tumor, that grows in such a speed ... My point of view. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Cheri Miller Gesendet: Freitag, 23. M?rz 2007 14:44 An: 'Marshall Terry Dr,Consultant Histopathologist'; 'Akemi Allison-Tacha'; 'Rene J Buesa'; 'Galiotto, Laura'; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] delivery time of last tray of H&E Turn around time Terry. For us it's about getting the slides to the Pathologist early so they may order whatever additional testing needed and still get a hard copy report to the Clinician by the end of the day. We have 24 hour turnaround for most cases. Cheri Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Thursday, March 22, 2007 12:28 PM To: Akemi Allison-Tacha; Rene J Buesa; Galiotto, Laura; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] delivery time of last tray of H&E This is all bloody unbelievable. Don't any of you sleep? What is the point of working at night? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: 22 March 2007 17:25 To: Rene J Buesa; Galiotto, Laura; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] delivery time of last tray of H&E Ren?--That was similar to the protocol I followed in the old days before 24/6 . We provided rush's 1st, followed by bx's by 8AM, we did however, provide special stains and recuts and IHC's by 2-3 PM, depending if it wasn't a lengthy procedure. If you have off site facilities to provide for, that is a different situation. You have to work around courier sheduals for delivering to off site facilities. Everyone needs their slides and time is money!! With the new microwave processing technology that is now in use, there has been a shift to having the majority of staff starting at 10 PM at night. MUCH TO THE DISLIKING OF HISTOTECH"S. The day shift is minimum. IHC is done during the day shift, after the pathologist has determined to do so. In most situations, a battery of IHC's tests are pre-set, as well as with special stains. Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting & Staffing Specializing in Histology, SS, IHC, & Microarray Madison, WI Tele: (925) 788-0900 E-Mail: akemiat3377@yahoo.com --- Rene J Buesa wrote: > Laura: > For a laboratory I used to manage with quite similar annual workload > we had the following > schedule: > 1- last tissue accepted at 7:00 PM; > 2- two VIPs atarting with delay to be finished at 4:00 AM next day; > 3- Rushes ready for the pathologists at 8:00 AM next day; > 4-All slides ready, the latest, at 1:00 PM. > 5- All IHC ready by 2 PM > 6- all HC ready by noon. > Ren? J. > > "Galiotto, Laura" wrote: > Hello Fellow Histo Tech's, > Will anyone be willing to share the delivery time of the last tray of > H&E for the days workload to the pathologist reading them? > What is your cutoff time for specimen acceptance? > What is your annual case workload? > I am trying to improve the TAT from specimen gross to the H&E > delivered to the pathologist. Unless we change the cutoff time I can > not see how I can do this? Any suggestions? > We process 22,000 cases a year an average of 280 blks a day consisting > of types of tissue. > Please Help > Laura > > > ******************* PLEASE NOTE ******************* This > E-Mail/telefax message and any documents accompanying this > transmission may contain information that is privileged, confidential, > and/or exempt from disclosure under applicable law and is intended > solely for the addressee(s) named above. If you are not the intended > addressee/recipient, you are hereby notified that any use of, > disclosure, copying, distribution, or reliance on the contents of this > E-Mail/telefax information is strictly prohibited and may result in > legal action against you. Please reply to the sender advising of the > error in transmission and immediately delete/destroy the message and > any accompanying documents. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Sucker-punch spam with award-winning protection. > Try the free Yahoo! Mail Beta. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tam_melville <@t> yahoo.com Fri Mar 23 22:01:33 2007 From: tam_melville <@t> yahoo.com (Tamara Melville) Date: Fri Mar 23 22:01:35 2007 Subject: [Histonet] Remove me from histonet email list In-Reply-To: <6966B0AB401CE845B977613354CEDA91254C77@lap-ex-01.lapitoy.fi> Message-ID: <753520.21501.qm@web38010.mail.mud.yahoo.com> Hello, Please, remove from Histonetters email lists. Thank you. Tamara Parkkisenniemi Paivi LSHP wrote: Hello, Please, remove from Histonetters email lists. Thank you. P?ivi _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- No need to miss a message. Get email on-the-go with Yahoo! Mail for Mobile. Get started. From jpastor1 <@t> nycap.rr.com Sat Mar 24 14:53:50 2007 From: jpastor1 <@t> nycap.rr.com (joseph pastore) Date: Sat Mar 24 14:53:36 2007 Subject: [Histonet] H&E artifact Message-ID: <000e01c76e4e$25029860$7864a8c0@JGPONLINE> Hi Guys and Girls, I really need some help here.We have been experiencing some artifact in our H&E stains. What it is are patches where the nuclei are very pale ble...that is the nuclei are just empty and a very pale blue and show no chromatin. Now tis is not throughout the section....only in spots. It also seems restricted to the biopsies. The routine stuff is fine. I mean in a batch of slides with both regular surgical specimens and biopsies, the artifact would be in only some of the biopsies not the routine stuff and not all the biopsies. Now at first we thought it was the stain. I checked that and whent through several variations but still the same. Then we looked at the processor. My Excelsior got rolly skrewed up in a recent power outage So we gutted it and cleaned it out. During this time We used our old Leica processor. This worked like a charm. Until last Fri. The pathologist noted that 3 of the biopsies showed the artifact. OK I was on leave but my tech said that the pathologist said the rest were fine. This week everything was fine until Fri. Again the routine specimens were OK but I saw this artifact in the biopsies. I really need some input here From rjbuesa <@t> yahoo.com Sat Mar 24 15:30:14 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Mar 24 15:30:18 2007 Subject: [Histonet] H&E artifact In-Reply-To: <000e01c76e4e$25029860$7864a8c0@JGPONLINE> Message-ID: <678588.11016.qm@web61220.mail.yahoo.com> The "empty nuclei" are very likely due to the sections NOT drained before they are dryed in the oven. Place the slides vertical (against the water bath for instance) for a few minutes. Then gently shake them to eliminate any water that may remain in the slide (and underneath the section) BEFORE taking the slides to the drying oven. If you go to HistoNet archieves you will find previous discussions on this "drying artifact". Ren? J. joseph pastore wrote: Hi Guys and Girls, I really need some help here.We have been experiencing some artifact in our H&E stains. What it is are patches where the nuclei are very pale ble...that is the nuclei are just empty and a very pale blue and show no chromatin. Now tis is not throughout the section....only in spots. It also seems restricted to the biopsies. The routine stuff is fine. I mean in a batch of slides with both regular surgical specimens and biopsies, the artifact would be in only some of the biopsies not the routine stuff and not all the biopsies. Now at first we thought it was the stain. I checked that and whent through several variations but still the same. Then we looked at the processor. My Excelsior got rolly skrewed up in a recent power outage So we gutted it and cleaned it out. During this time We used our old Leica processor. This worked like a charm. Until last Fri. The pathologist noted that 3 of the biopsies showed the artifact. OK I was on leave but my tech said that the pathologist said the rest were fine. This week everything was fine until Fri. Again the routine specimens were OK but I saw this artifact in the biopsies. I really need some input here _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a PS3 game guru. Get your game face on with the latest PS3 news and previews at Yahoo! Games. From Lynne.Bell <@t> hitchcock.org Mon Mar 26 07:14:10 2007 From: Lynne.Bell <@t> hitchcock.org (Bell, Lynne) Date: Mon Mar 26 07:14:14 2007 Subject: [Histonet] Hollande's solution Message-ID: We are thinking of changing from Bouin's solution to Hollande's solution for our GI biopsies. I have looked at the MSDS and do not see any real issues. My biggest question is disposal of this solution. Can it safely go down the drain? Also, Freida Carson's book suggests washing the specimens before processing them. Does everyone do that? Thanks for any and all help. Lynne A. Bell, HT (ASCP) Central Vermont Medical Center P. O. Box 547 130 Fisher Road Barre, VT 05641 802-371-4923 From Eric.C.Kellar <@t> questdiagnostics.com Mon Mar 26 07:37:45 2007 From: Eric.C.Kellar <@t> questdiagnostics.com (Kellar, Eric C) Date: Mon Mar 26 07:38:08 2007 Subject: [Histonet] Hollande's solution Message-ID: <6843061CE6B98E4B96590D4F299618F80271D267@qdcws0117.us.qdx.com> Lynne, This is a modification of Bouin's solution. It is stable and will decalcify small bone specimens. Tissue that is fixed with Hollande's can be stained successfully with most stains, and the cupric acetate in the solution stabilizes red blood cell membranes and eosinophil and endocrine cell granules so that less lysis occurs than with Bouin's solution. Thorough washing of the fixative prior to placing the specimen in a phosphate buffered formalin solution is necessary because the salts present in the solution will form an insoluble phosphate precipitate. Eric C. Kellar Quest Diagnostics Histology Laboratory Supervisor South Florida -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bell, Lynne Sent: Monday, March 26, 2007 8:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hollande's solution We are thinking of changing from Bouin's solution to Hollande's solution for our GI biopsies. I have looked at the MSDS and do not see any real issues. My biggest question is disposal of this solution. Can it safely go down the drain? Also, Freida Carson's book suggests washing the specimens before processing them. Does everyone do that? Thanks for any and all help. Lynne A. Bell, HT (ASCP) Central Vermont Medical Center P. O. Box 547 130 Fisher Road Barre, VT 05641 802-371-4923 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ============================================================================== The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. ============================================================================== From drgeoffreywhite <@t> yahoo.com Mon Mar 26 08:13:06 2007 From: drgeoffreywhite <@t> yahoo.com (Geoffrey) Date: Mon Mar 26 08:13:13 2007 Subject: [Histonet] cover slipping by hand Message-ID: <100814.58787.qm@web63501.mail.re1.yahoo.com> Hi Histonetters, does anyone have a good procedure for cover slipping by hand? At the time our research group use normal glass cover slips. As everyone know this are not really the finest art of work. After I used Goggle, I found a new kind of cover slips, names DiscoverSlip. Have anyone heard or used this product? Thanks for your help. --------------------------------- TV dinner still cooling? Check out "Tonight's Picks" on Yahoo! TV. From Richard.Breckenridge <@t> uth.tmc.edu Mon Mar 26 10:45:18 2007 From: Richard.Breckenridge <@t> uth.tmc.edu (Breckenridge, Richard A) Date: Mon Mar 26 10:45:23 2007 Subject: [Histonet] Job opening at UT Health Science Center- Houston Message-ID: Hello Everyone, We have a new position opening here and any help in filling it would be most appreciated. We are opening a new morphoproteomic/ image analysis laboratory. I need a tech that has IHC and image analysis experience. We may be willing to train the right person to do image analysis if other experience is satisfactory. Please inquire and apply at www.uthouston.edu . Follow the links to job openings. The position is a Research Associate, job code 4411. Salary is negotiable. Thanks. Richard A. Breckenridge, HT(ASCP) Technical Director/ Chief Histology Technician University of Texas- Houston Medical School Histology/ IHC Laboratory Office 713-500-6792 IHC Lab 713-500-5096 Histology Lab 713-500-5363 FAX 713-500-0733 Email - Richard.Breckenridge@uth.tmc.edu From POWELL_SA <@t> Mercer.edu Mon Mar 26 11:10:22 2007 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Mon Mar 26 11:24:04 2007 Subject: [Histonet] Question about historical photographs In-Reply-To: Message-ID: <01MEOA1ZH8468WYB5K@Macon2.Mercer.edu> Hi James, I have just returned from attending a great meeting of Region III in NC. We had a great turn out. Wanda Grace Jones presented almost the same named presentation. It was titled "IHC, Past, present and Future Where we have been and Where we are going" in which he had many images of the historical founders of histology/IHC. She is the new president of NCSHT. She may be able to help you with this. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Happel, James F. Sent: Friday, March 23, 2007 11:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question about historical photographs Good Day, I am putting together a presentation on the past, present and future of histology/pathology and am looking for historical pictures, drawings, advertisements, etc. to include in the presentation. Does anyone have images I could use or know where I can find some? Thank you. James Happel Massachusetts General Hospital The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> aol.com Mon Mar 26 13:50:01 2007 From: rsrichmond <@t> aol.com (rsrichmond@aol.com) Date: Mon Mar 26 13:50:25 2007 Subject: [Histonet] Re: Hollande's fixative In-Reply-To: <200703261401.c346080a6f276@rly-mb01.mail.aol.com> References: <200703261401.c346080a6f276@rly-mb01.mail.aol.com> Message-ID: <8C93DEEB1ED3412-D74-A087@webmail-da14.sysops.aol.com> Lynne A. Bell, HT (ASCP) at Central Vermont Medical Center in Barre VT asks: >>We are thinking of changing from Bouin's solution to Hollande's solution for our GI biopsies. I have looked at the MSDS and do not see any real issues. My biggest question is disposal of this solution. Can it safely go down the drain? Also, Freida Carson's book suggests washing the specimens before processing them. Does everyone do that?<< and Eric C. Kellar, Histology Laboratory Supervisor at Quest Diagnostics in South Florida replies that Hollande's fixative >>is stable and will decalcify small bone specimens. Tissue that is fixed with Hollande's can be stained successfully with most stains, and the cupric acetate in the solution stabilizes red blood cell membranes and eosinophil and endocrine cell granules so that less lysis occurs than with Bouin's solution.Thorough washing of the fixative prior to placing the specimen in a phosphate buffered formalin solution is necessary because the salts present in the solution will form an insoluble phosphate precipitate.<< **************************** HOLLANDE'S FIXATIVE: Water 220 mL Cupric acetate 25 g Picric acid, saturated aqueous 780 mL Formalin 37% 100 mL Acetic acid, glacial 10 mL Dissolve the cupric acetate into the water. Add the picric acid and filter. Add the rest of the ingredients and mix. Original reference, cited at Stainsfile: Hollande: Comptes rendus hebdomadaires des s?ances et m?moires de la soci?t? de biologie [Paris] 1918;81:17. -- R.D. Lillie (3rd ed., 1965) cites Hartz, Am J Clin Path 1947;17:50 - the complete 1918 reference might be available here. present reference: http://stainsfile.info/StainsFile/prepare/fix/copper/hollande.htm Note that Bryan D. Llewellyn, compiler of Stainsfile, has recalculated the formula to use ready-made saturated picric acid in water rather than dry chemical, which because of its explosion hazard should not be in your laboratory! The formula is in Freida Carson's book, but I've just lent my copy to a couple of histotechs studying for the registry exam. Hollande's fixative cannot go down the drain because of the formaldehyde, the picric acid, and possibly the copper. (You should look for a better MSDS than the one you have.) Pre-packaged bottles of it are available commercially. In the many years I've done locum tenens pathology in many different laboratories, I have never seen Hollande's fixative in use. I'd be reluctant to handle it because of the picric acid, which is not only toxic and (when in anhydrous form) explosive, but which stains fingers, clothing, and anything else it can get to. If you were going to use Hollande's fixative, you would have to investigate its compatibility with your immunostains. Bob Richmond Samurai Pathologist Knoxville TN March 26th, 2007 ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From thoward <@t> unm.edu Mon Mar 26 14:17:39 2007 From: thoward <@t> unm.edu (Tamara A Howard) Date: Mon Mar 26 14:17:46 2007 Subject: [Histonet] tape transfer paraffin question Message-ID: Help! I've been given 2 tissue array slides that were made using one of the tape transfer systems. I've never had tape slides - isn't the tape supposed to be removed before staining? The supplier of the slides says to just proceed as usual, but these look to me as if they still have tape, and I can see the "this side to adhesive" & "knife edge" (or whatever the other message is) - shouldn't those messages be gone if the tape has been removed? Maybe they are supposed to look this way, and I'm just being paranoid (they are ungodly expensive samples). Thanks! Tamara *************************** Tamara Howard Cell Biology & Physiology UNM-HSC Albuquerque, NM *************************** From meligroc <@t> zgi.com Mon Mar 26 14:42:56 2007 From: meligroc <@t> zgi.com (CRME (Criss Meligro)) Date: Mon Mar 26 14:43:15 2007 Subject: [Histonet] unsucribe Message-ID: <746E68D5CBB205409B12EC32A61EE4010249885D@ned.zgi.com> From tp2 <@t> medicine.wisc.edu Mon Mar 26 14:59:00 2007 From: tp2 <@t> medicine.wisc.edu (Thomas Pier) Date: Mon Mar 26 15:00:38 2007 Subject: [Histonet] tape transfer paraffin question Message-ID: <4607DFB4020000DF000060C8@gwmail.medicine.wisc.edu> Sometimes those parts of the tape stick to the slide. As long as the pink tape is gone, the area over the cores should be free of tape. Tom Pier >>> "Tamara A Howard" 03/26/07 2:17 PM >>> Help! I've been given 2 tissue array slides that were made using one of the tape transfer systems. I've never had tape slides - isn't the tape supposed to be removed before staining? The supplier of the slides says to just proceed as usual, but these look to me as if they still have tape, and I can see the "this side to adhesive" & "knife edge" (or whatever the other message is) - shouldn't those messages be gone if the tape has been removed? Maybe they are supposed to look this way, and I'm just being paranoid (they are ungodly expensive samples). Thanks! Tamara *************************** Tamara Howard Cell Biology & Physiology UNM-HSC Albuquerque, NM *************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From atebo <@t> aahs.org Mon Mar 26 15:31:41 2007 From: atebo <@t> aahs.org (Tebo, Andrea) Date: Mon Mar 26 15:31:48 2007 Subject: [Histonet] unsubscribe Message-ID: <00790F10D0600A41AEE8899901E533A61044B587@aamcexch.aamc.org> Please remove me from the list for now. I will re-subscribe at a later date. Thanks so much, Andrea Tebo ---------------------------------------------------------------------- This message [including any attachments] contains information intended for a specific individual[s] and purpose that may be confidential or otherwise legally protected from disclosure. Any review, use, distribution, disclosure of contents, or copying of the message is strictly prohibited. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately without copying or disclosing the information. From gayatrikrishna <@t> msn.com Mon Mar 26 18:50:06 2007 From: gayatrikrishna <@t> msn.com (Gayatri Krishna) Date: Mon Mar 26 18:50:12 2007 Subject: [Histonet] will do at a later time. Message-ID: Hello Plese remove my name from the histonet list. I will subscribe again at a later date. thanks gayatri From tissuearray <@t> hotmail.com Tue Mar 27 07:24:10 2007 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Tue Mar 27 07:24:22 2007 Subject: [Histonet] tape transfer paraffin question Message-ID: The tape transfer system often leaves a resin on the slides. If folks don't soak the tape in the solution that removes the tape then you will get left over glue and possibly tape left on the slide. I don't recommend the tape method for cutting array blocks. It is mostly used by techs who are either inexperienced or have a hard time cutting array blocks. The samples are often uninterpretable because of the Tape Sectioning Aid System. Thom arrayworkshop.com ______________________________________________________________ From: "Thomas Pier" To: , Subject: Re: [Histonet] tape transfer paraffin question Date: Mon, 26 Mar 2007 14:59:00 -0500 MIME-Version: 1.0 Received: from swlx162.swmed.edu ([199.165.152.162]) by bay0-mc8-f4.bay0.hotmail.com with Microsoft SMTPSVC(6.0.3790.2668); Mon, 26 Mar 2007 13:00:59 -0700 Received: from localhost ([127.0.0.1] helo=swlx162.swmed.edu)by swlx162.swmed.edu with esmtp (Exim 4.34)id 1HVvMt-0001Bv-3I; Mon, 26 Mar 2007 15:00:39 -0500 Received: from [199.242.236.160] (helo=swlx160.swmed.edu)by swlx162.swmed.edu with esmtp (Exim 4.34) id 1HVvMr-0001Bq-6afor histonet@lists.utsouthwestern.edu; Mon, 26 Mar 2007 15:00:37 -0500 Received: from lists.medicine.wisc.edu ([128.104.208.5]helo=mailout.medicine.wisc.edu)by swlx160.swmed.edu with esmtp (Exim 4.62)(envelope-from ) id 1HVvMn-0008Cx-3Vfor histonet@lists.utsouthwestern.edu; Mon, 26 Mar 2007 15:00:37 -0500 Received: from gwmail.medicine.wisc.edu (gwmail.medicine.wisc.edu[128.104.208.34])by mailout.medicine.wisc.edu (8.13.1/8.13.1) with ESMTP idl2QJxFCi032290for ; Mon, 26 Mar 2007 14:59:20 -0500 Received: from Medicine1-MTA by gwmail.medicine.wisc.eduwith Novell_GroupWise; Mon, 26 Mar 2007 14:59:17 -0500 >Sometimes those parts of the tape stick to the slide. As long as the >pink tape is gone, the area over the cores should be free of tape. > >Tom Pier > > >>> "Tamara A Howard" 03/26/07 2:17 PM >>> >Help! > >I've been given 2 tissue array slides that were made using >one of the tape transfer systems. I've never had tape >slides - isn't the tape supposed to be removed before >staining? The supplier of the slides says to just proceed >as usual, but these look to me as if they still have tape, >and I can see the "this side to adhesive" & "knife edge" >(or whatever the other message is) - shouldn't those >messages be gone if the tape has been removed? Maybe they >are supposed to look this way, and I'm just being paranoid >(they are ungodly expensive samples). > >Thanks! > >Tamara > > >*************************** >Tamara Howard >Cell Biology & Physiology >UNM-HSC >Albuquerque, NM >*************************** > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ [1]The average US Credit Score is 675. The cost to see yours: $0 by Experian. References 1. http://g.msn.com/8HMAENUS/2743??PS=47575 From rjbuesa <@t> yahoo.com Tue Mar 27 08:53:46 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 27 08:53:53 2007 Subject: [Histonet] Safety survey. Message-ID: <44843.99045.qm@web61216.mail.yahoo.com> Dear Colleagues: I am conducting a survey about safety in the histology laboratory. Surprisingly enough the initial tabulation is showing that the histology environment is not as safe as we all thought it was. The more answers I get the better it will be to understand our safety characteristics. Would you like to participate? I you do please e-mail me and I will send you the quesionnaire. As soon as the results are completed I will send you the results. Ren? J. --------------------------------- Looking for earth-friendly autos? Browse Top Cars by "Green Rating" at Yahoo! Autos' Green Center. From christiegowan <@t> msn.com Tue Mar 27 09:14:49 2007 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Tue Mar 27 09:15:00 2007 Subject: [Histonet] Positive IF controls Message-ID: Does anyone know of a company that supplies positive immunofluorescence controls? Thanks, Christie From nic.leipzig <@t> utoronto.ca Tue Mar 27 09:46:37 2007 From: nic.leipzig <@t> utoronto.ca (Nic Leipzig) Date: Tue Mar 27 09:47:29 2007 Subject: [Histonet] Neuron specific beta III tubulin antibody Message-ID: We are looking for an antibody for IHC on paraformaldehyde fixed cells (differentiated rat neurospheres) that will stain only neurons. We have had recent troubles with glial cells staining positive with beta III tubulin antibodies. Up until ~5 months ago we had an antibody to beta III tubulin (TUJ1) from Chemicon that worked beautifully for differentiated Rat (Sprague Dawley) neurospheres. Then the lots changed and it doesn't work at all and they can't do anything about it besides sending us more vials of the same lot. So we have tried a beta III tubulin TUJ1 antibody from Stem Cell Technologies, but this stains neurons and glial cells (mostly astrocytes). Sigma's SDL clone has even more background staining. Any suggestions are greatly appreciated. Thanks, Nic **************************************************************** Nic Leipzig, PhD Postdoctoral Research Fellow University of Toronto Donnelly Centre for Cellular and Biomolecular Research Shoichet Lab, 530-160 College Street Toronto, ON M5S 3E1 Telephone: (416) 978-0352 Voicemail: (416) 978-6924 x224 E-mail: nic.leipzig@utoronto.ca **************************************************************** From sbreeden <@t> nmda.nmsu.edu Tue Mar 27 10:29:00 2007 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Mar 27 10:29:09 2007 Subject: [Histonet] Temp/Traveler Agencies Message-ID: <4D14F0FC9316DD41972D5F03C070908B8F43C5@nmdamailsvr.nmda.ad.nmsu.edu> Could agencies that handle temps/travelers/per diem registered techs please contact me OFFLINE? I am investigating the possibility of this type of coverage during a vacation period. The information gathered will be presented to management for review. Please do not reply via Histonet. Thank you! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 From stamptrain <@t> yahoo.com Tue Mar 27 11:15:31 2007 From: stamptrain <@t> yahoo.com (Roger Moretz) Date: Tue Mar 27 11:15:44 2007 Subject: [Histonet] Positive IF controls In-Reply-To: Message-ID: <716810.90272.qm@web55812.mail.re3.yahoo.com> We have used Newcomer supply http//:newcomersupply.com and are satisfied customers. Depends what you need , of course. No financial interest, just a customer. Roger Moretz, Ph.D. Dept of Toxicology Boehringer Ingelheim Pharmaceuticals, Inc. Ridgefield, CT --- CHRISTIE GOWAN wrote: > Does anyone know of a company that supplies positive > immunofluorescence > controls? > Thanks, > Christie > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ____________________________________________________________________________________ It's here! Your new message! Get new email alerts with the free Yahoo! Toolbar. http://tools.search.yahoo.com/toolbar/features/mail/ From cervantes <@t> bendres.com Tue Mar 27 11:48:30 2007 From: cervantes <@t> bendres.com (Cervantes, Jessica) Date: Tue Mar 27 11:48:44 2007 Subject: [Histonet] Problem with intestinal epithelium In-Reply-To: References: <003401c76d69$aa75a9a0$6501a8c0@Patsy><003c01c76d6a$adacc350$6501a8c0@Patsy> Message-ID: <8943D65F9AD70E4488AD6DE09F15088768C402@BRIEX04A> Rene, Patsy, Jennifer, and Ruth- I followed this thread with some interest as we have a project in which we'd like to preserve the mucosal layer in rat intestines. Would any of you be open to sending me a protocol of the steps following the formalin fix? I'd like to know specifically what kind of formalin you are using (percentage, buffer), and what stains (if any). We have tried PAS/Alcian blue staining for the mucous, but since the mucosal layer was destroyed during our sample prep, we only saw mucous in the goblet cells. Any details you can provide will be much appreciated. Thank you, Jessica Cervantes Bend Research, Inc Bend, OR 97701 (541) 382-4100 cervantes@bendres.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Wednesday, March 21, 2007 10:10 AM To: Mikael Niku Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Problem with intestinal epithelium We get bovine intestine from a local university that has an agriculture program. We provide them with a large container of formalin and ask that they open the intestine and place it immediately in the formalin. We have had great preservation of the epithelium. The piece of intestine is about 8-10 inches long. Jennifer Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu Mikael Niku Sent by: histonet-bounces@lists.utsouthwestern.edu 03/21/2007 03:49 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Problem with intestinal epithelium Dear Histonetters, we are trying to make good-quality sections (paraffin and cryo) of bovine intestines. The problem is that the epithelium seems to be stripped off whatever we do. This seems to be a problem especially with slaughterhouse material, although the delay from death to sampling is less than 30 min. Currently we are cutting the intestine to pieces, washing with PBS buffer, and fixing immediately in buffered paraformaldehyde or freezing in isopentane / liquid nitrogen. Any ideas, anyone? Or are we just too late here, could the mucosa be destroyed so soon after death? With best regards, Mikael Niku -- //////////////////////////////////////////////////////////// Mikael Niku URL: www.helsinki.fi/~mniku/ University of Helsinki Dept. Basic Veterinary Sciences - Mit?k? mielt? olen l?nsimaisesta sivistyksest?? Minusta se olisi erinomainen ajatus! - Gandhi //////////////////////////////////////////////////////////// _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Eric.C.Kellar <@t> questdiagnostics.com Tue Mar 27 13:16:47 2007 From: Eric.C.Kellar <@t> questdiagnostics.com (Kellar, Eric C) Date: Tue Mar 27 13:17:03 2007 Subject: [Histonet] Histotechnologist Job Opportunity at Quest Diagnostics in South Florida Message-ID: <6843061CE6B98E4B96590D4F299618F801583C13@qdcws0117.us.qdx.com> There's quite a distance between wondering and knowing. And for patients waiting for answers to important health questions, it's a road they want to travel as quickly as possible. At Quest Diagnostics Incorporated, we understand urgency. But more than speed, we focus our energies on accuracy. Currently we are seeking a Histotechnologist I. As a Histotechnologist I, you will perform the daily activities as described below: 1. Capable of performing all of the duties/responsibilities of a Histotechnician I and II. 2. Ensure proper accessioning and labeling of all tissue samples. 3. Process paperwork associated with accessioning and reporting. 4. Ensure proper tissue processing. 5. Embed processed tissue in paraffin. 6. Perform microtomy of embedded tissue. 7. Prepare slides for routine Hematoxylin and Eosin staining. 8. Perform coverslipping of stained slides either manually or automated. 9. Prepare solutions and reagents for special stain procedures. 10. Obtain and validate tissue used in special stains. 11. Perform all special stain procedures. 12. May prepare solutions and reagents for IHC procedures. 13. May obtain and validate control material used in IHC procedures. 14. May perform IHC testing. 15. Perform filing of finished blocks and slides. 16. Perform routine maintenance and cleaning of equipment and troubleshoot minor equipment failures. Document remedial actions such as repairs or repeated tests. 17. Provide training and guidance to Histotechnicians, students and lab aides. 18. Adhere to laboratory's quality control policies and document all quality control activities. 19. Ensure all corporate safety, quality control and quality assurance standards are met. 21. Ensure compliance with all local, federal, CLIA and CAP regulations. 22. Maintain a clean and well-organized work area. 23. Other duties, as assigned by supervisor. Shift: Day M-F. No weekends or Holidays Education: AA, AS or BS degree or equivalent training and experience. Work Experience: 1-3 years experience as a Histotechnician or Histotechnologist. Special Requirements: HT/HTL (ASCP), prefer QIHC (ASCP) and Florida State Licensure as a Clinical Laboratory Technician/Technologist. Quest Diagnostics has many career opportunities for individuals whose talent, initiative and dedication will complement our belief that the patient comes first and that values do matter. We work to earn our customers' trust every day by providing the highest quality products and services in a professional, accessible and informative way. Our workforce is diverse and talented and believes in our vision: "Dedicated people improving the health of patients through unsurpassed diagnostic insights." Please contact Human Resources at 954-378-5000 or on the web at http://www.questdiagnostics.com ============================================================================== The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. ============================================================================== From carl.hobbs <@t> kcl.ac.uk Tue Mar 27 13:38:34 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Tue Mar 27 13:39:08 2007 Subject: [Histonet] re: neurone specific beta III Message-ID: <000501c7709f$20a71400$4101a8c0@carlba65530bda> Two examples of Suppliers: Abcam : both their current TUJ1 Mouse and their polyclonal rabbit seem very good, IMHO. Covance: also do a very good TUJ1-like rabbit poly. I haven't seen astrocyte staining in any of the above in tissue sections: I would be grateful for your feedback, if you try out any of the above, on cells Carl From jnocito <@t> satx.rr.com Tue Mar 27 13:59:50 2007 From: jnocito <@t> satx.rr.com (jnocito@satx.rr.com) Date: Tue Mar 27 14:00:05 2007 Subject: [Histonet] Positive IF controls In-Reply-To: References: Message-ID: Christie, what I had to do was get a fresh tonsil and use that. My understanding is that companies do not want the liabilty of the controls not working. Too much can go wrong during shipping, etc. JTT ----- Original Message ----- From: CHRISTIE GOWAN Date: Tuesday, March 27, 2007 9:18 am Subject: [Histonet] Positive IF controls To: histonet@lists.utsouthwestern.edu > Does anyone know of a company that supplies positive > immunofluorescence > controls? > Thanks, > Christie > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Melissa.Gonzalez <@t> cellgenesys.com Tue Mar 27 15:01:44 2007 From: Melissa.Gonzalez <@t> cellgenesys.com (Melissa Gonzalez) Date: Tue Mar 27 15:01:55 2007 Subject: [Histonet] RE: Positive IF controls In-Reply-To: <20070327182236.1E36027F058@mail.cellgenesys.com> References: <20070327182236.1E36027F058@mail.cellgenesys.com> Message-ID: Christie, if you mean, pre-stained slides, Chroma makes a pack of 4 slides with tissues stained immunofluorescently to use as positive control checks for your equipment. Invitrogen also makes them but they are fluorescent beads, and IMO, are way too bright and not especially realistic. Melissa ------------------------------------------ Message: 9 Date: Tue, 27 Mar 2007 14:14:49 +0000 From: "CHRISTIE GOWAN" Subject: [Histonet] Positive IF controls To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; format=flowed Does anyone know of a company that supplies positive immunofluorescence controls? Thanks, Christie From JMacDonald <@t> mtsac.edu Tue Mar 27 17:33:30 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Mar 27 17:33:37 2007 Subject: [Histonet] Problem with intestinal epithelium In-Reply-To: <8943D65F9AD70E4488AD6DE09F15088768C402@BRIEX04A> Message-ID: Jessica, After the intestine was put into the formalin we left it for a day or two minimum. It was opened to ensure that the formalin reached the epithelium. Remember, we had bovine intestine. We took samples from the intestine, just as you would for a human intestine. They were processed overnight and embedded on edge to show all layers. We did H&E and mucicarmine staining on them. The epithelium was fully intact. Jennifer Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu "Cervantes, Jessica" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/27/2007 09:48 AM To cc Subject RE: [Histonet] Problem with intestinal epithelium Rene, Patsy, Jennifer, and Ruth- I followed this thread with some interest as we have a project in which we'd like to preserve the mucosal layer in rat intestines. Would any of you be open to sending me a protocol of the steps following the formalin fix? I'd like to know specifically what kind of formalin you are using (percentage, buffer), and what stains (if any). We have tried PAS/Alcian blue staining for the mucous, but since the mucosal layer was destroyed during our sample prep, we only saw mucous in the goblet cells. Any details you can provide will be much appreciated. Thank you, Jessica Cervantes Bend Research, Inc Bend, OR 97701 (541) 382-4100 cervantes@bendres.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Wednesday, March 21, 2007 10:10 AM To: Mikael Niku Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Problem with intestinal epithelium We get bovine intestine from a local university that has an agriculture program. We provide them with a large container of formalin and ask that they open the intestine and place it immediately in the formalin. We have had great preservation of the epithelium. The piece of intestine is about 8-10 inches long. Jennifer Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu Mikael Niku Sent by: histonet-bounces@lists.utsouthwestern.edu 03/21/2007 03:49 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Problem with intestinal epithelium Dear Histonetters, we are trying to make good-quality sections (paraffin and cryo) of bovine intestines. The problem is that the epithelium seems to be stripped off whatever we do. This seems to be a problem especially with slaughterhouse material, although the delay from death to sampling is less than 30 min. Currently we are cutting the intestine to pieces, washing with PBS buffer, and fixing immediately in buffered paraformaldehyde or freezing in isopentane / liquid nitrogen. Any ideas, anyone? Or are we just too late here, could the mucosa be destroyed so soon after death? With best regards, Mikael Niku -- //////////////////////////////////////////////////////////// Mikael Niku URL: www.helsinki.fi/~mniku/ University of Helsinki Dept. Basic Veterinary Sciences - Mit?k? mielt? olen l?nsimaisesta sivistyksest?? Minusta se olisi erinomainen ajatus! - Gandhi //////////////////////////////////////////////////////////// _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From holmberg.katie <@t> gmail.com Tue Mar 27 19:07:59 2007 From: holmberg.katie <@t> gmail.com (katie holmberg) Date: Tue Mar 27 19:08:04 2007 Subject: [Histonet] traveling histotech Message-ID: Does anyone have any experience or insight as a traveling histotech? Although this isn't a technical question, I would greatly appreciate any input. From sheila_adey <@t> hotmail.com Tue Mar 27 19:53:29 2007 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Tue Mar 27 19:53:35 2007 Subject: [Histonet] IHC buffers Message-ID: Hello All, We currently have 2 IHC stainers up and running while we are doing our cross over studies. (We are transitioning from the Artisan to the Biogenex i6000) My question is; we use a Tris buffer with the Artisan and we use PBS with the i6000, is it ok to allow slides that may have sat in one during the prestaining period to then be exposed to the other during staining? They are both the same ph, I just don't want any unnecessary variables but I know this has happened with some slides. Any opinions? Thanks in advance Sheila Adey HT MLT Port Huron Hospital Michigan _________________________________________________________________ RealLiveMoms: Share your experience with Real Live Moms just like you http://www.reallivemoms.ca/ From koellingr <@t> comcast.net Tue Mar 27 20:09:58 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Tue Mar 27 20:10:03 2007 Subject: [Histonet] A hepatoma cell line grow in mouse liver, How can I distinguish these two cells? Message-ID: <032820070109.22942.4609C066000A0E8E0000599E22070032019D09020704040A0105@comcast.net> Yan Gao, This is a great way to do it as has been described by Jackie with the one caveat that even if you are doing this with SCID mice, remember that you can have some background problems depending on the genetic background of the SCID. B & T cell "leakage" in SCID's is a well known fact due to some small amount of residual immunocompentant activity. Indeed, plasma Ig can be found in SCIDS, so B cells can certainly be there. Depends on the genetic background (CB.17, C3H, ICR, etc). Some are very clean (no leakage) so you would not have to worry about putting a mouse monoclonal on a SCID when using xenografts. But some are very "leaky" and so mouse Ig antibodies will give you a bit of a problem. Depends on your mouse model/background strain. Ray Koelling Phenopath Laboratories Seattle, WA -------------- Original message -------------- From: "Jackie M O'Connor" > Human mitochondria marker will stain only human cells. > Chemicon - catalog number MAB1273. Your mouse is immunocompromised, i.e., > SCID? No need to block mouse Ig to use this mAB. > I use this constantly to distinguish human tumor xenografts in mice. > > > > "yan gao" > Sent by: histonet-bounces@lists.utsouthwestern.edu > 03/19/2007 08:49 AM > > To > histonet@lists.utsouthwestern.edu > cc > > Subject > [Histonet] A hepatoma cell line grow in mouse liver, How can I > distinguish these two cells? > > > > > > > > Hi, Histonet. > > We have a human hepatoma cell line injected into mouse liver. The > cell line grown in mouse liver for three weeks. The human hepatoma > cells mixed into mouse liver. Does anyone have any idea how to > distinguish the human hepatoma cell line in the mouse liver? What > marker I should use to distinguish two kinds of cells? Thanks. > > > Yan Gao > _________________________________________________________________ > > [1]Get a FREE Web site, company branded e-mail and more from Microsoft > Office Live! > > References > > 1. http://g.msn.com/8HMAENUS/2740??PS=47575 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hycento <@t> netscape.net Wed Mar 28 03:07:52 2007 From: hycento <@t> netscape.net (hycento@netscape.net) Date: Wed Mar 28 03:07:58 2007 Subject: [Histonet] Shandon Cadenza Message-ID: <8C93F27516C6FD0-1D64-2143@mblk-d42.sysops.aol.com> Hello. Anybody know where I can get a Reservoir, Slide Rack and Manual for a Shandon Cadenza? I have tried Thermo-Shandon already and they are unable to assist. Thanks, Hycento. ________________________________________________________________________ Check Out the new free AIM(R) Mail -- 2 GB of storage and industry-leading spam and email virus protection. From psanquin <@t> lugo.usc.es Wed Mar 28 07:09:30 2007 From: psanquin <@t> lugo.usc.es (Pablo =?iso-8859-1?Q?S=E1nchez?= Quinteiro) Date: Wed Mar 28 07:09:37 2007 Subject: [Histonet] Background In-Reply-To: <200703090009.l2909nsw065582@pro12.abac.com> References: <6.0.0.22.1.20070308165113.01b52aa0@gemini.msu.montana.edu> Message-ID: <3.0.6.32.20070328140930.010842f0@pop.lugo.usc.es> Dear Histonetters, I am doing OMP immunohistochemistry in paraffin-embedded sections of sheep olfactory mucosa. In my positive control -mouse olfactory tissue- there is not any background, but in he sheep sections I get lot of unspecific labelling (vessels, connective tissue, respiratory mucosae). Being my primary antibody raised in goat I guess that there is an undesired cross reaction with the sheep tissue. Could I improve my blocking? I do half an hour in 2% BSA and 5% Horse normal serum. Should I use Goat normal serum? Thanks in advance for your input. Pablo Sanchez-Quinteiro Lugo (Spain) From doug <@t> ppspath.com Wed Mar 28 08:23:05 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Wed Mar 28 07:21:02 2007 Subject: [Histonet] Paper Bag Message-ID: Hello All, I am looking for a supplier of the paper biopsy bags. Most of the sources that I use have discontinued the paper for the nylon bags. Thanks. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. From kmerriam2003 <@t> yahoo.com Wed Mar 28 07:37:01 2007 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Wed Mar 28 07:37:04 2007 Subject: [Histonet] human-on-human IHC staining Message-ID: <377116.32908.qm@web50307.mail.re2.yahoo.com> Hi, I did a quick search of the archives to look for information on the use of human antibodies on human tissue, but there was not much information there on this subject. We tried the Zenon kit (biotinylation) from invitrogen (as recommended by Chris van der Loos), but have not had much success as of yet. Any tips or advice would be appreciated. Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA ____________________________________________________________________________________ TV dinner still cooling? Check out "Tonight's Picks" on Yahoo! TV. http://tv.yahoo.com/ From anh2006 <@t> med.cornell.edu Wed Mar 28 07:57:19 2007 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Wed Mar 28 07:57:34 2007 Subject: [Histonet] human-on-human IHC staining In-Reply-To: <377116.32908.qm@web50307.mail.re2.yahoo.com> References: <377116.32908.qm@web50307.mail.re2.yahoo.com> Message-ID: For human on human staining several options work excellently in my hands: (1) Precomplexing primary with secondary antibody before adding to tissue (with appropriate blocking steps) (2) Biotinylating your primary with a kit beforehand (not Zenon exactly but instead biotinylate in larger quantities) (3) FITC labeling your primary (FluoReporter kit from InVitrogen) then use a rabbit anti-FITC "secondary" and an anti-rabbit polymer for amplification. Steps 2 & 3 require that you have sufficient primary in an appropriate concentration to conjugate. Are you sure the antibody you are working with is "good" for IHC ... ??? These kits unfortunately won't make miracles out of antibodies which are not optimized for IHC. I have not tried Zenon personally but have heard good things. If you want, send me your Zenon based protocol and maybe I can help. LMK if you need more details/information on any of the above. >Hi, > >I did a quick search of the archives to look for information on the >use of human antibodies on human tissue, but there was not much >information there on this subject. We tried the Zenon kit >(biotinylation) from invitrogen (as recommended by Chris van der >Loos), but have not had much success as of yet. Any tips or advice >would be appreciated. > >Kim > >Kim Merriam, MA, HT(ASCP) >Cambridge, MA > -- From akbitting <@t> geisinger.edu Wed Mar 28 08:11:44 2007 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Wed Mar 28 08:12:16 2007 Subject: [Histonet] HPV Family 16 controls Message-ID: <460A3150020000C900007B28@GHSGWIANW5V.GEISINGER.EDU> Histonetters, Now that Ventana has dc'd their HPV Family 16 control slides, I need to find a new source. Is anyone out there buying from another source? and would you mind sharing that info with me? Thanks, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From ROrr <@t> enh.org Wed Mar 28 10:12:18 2007 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Wed Mar 28 10:12:27 2007 Subject: [Histonet] Artifact book Message-ID: Hi Everyone, I have heard there is a book that has pictures of "histology artifacts" I'm thinking processing, cutting, staining everything. I'd like to have it for our students. Thanks Becky Orr CLA,HT(ASCP)QIHC Assistant Manager, Anatomic Pathology Evanston Northwestern Healthcare 847-570-2763 From doug <@t> ppspath.com Wed Mar 28 11:34:41 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Wed Mar 28 10:33:30 2007 Subject: [Histonet] Artifact book In-Reply-To: Message-ID: Histopathologic Methods And Color Atlas Of Special Stains And Tissue Artifacts by Lee G. Luna You can get it from... American Histolabs Inc 7605 Airpark Road Suite F, Gaithersburg, MD 20879 (866) 224-6298 Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Orr, Rebecca Sent: Wednesday, March 28, 2007 10:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Artifact book Hi Everyone, I have heard there is a book that has pictures of "histology artifacts" I'm thinking processing, cutting, staining everything. I'd like to have it for our students. Thanks Becky Orr CLA,HT(ASCP)QIHC Assistant Manager, Anatomic Pathology Evanston Northwestern Healthcare 847-570-2763 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Wed Mar 28 11:01:34 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Wed Mar 28 11:01:45 2007 Subject: [Histonet] Artifact book In-Reply-To: Message-ID: <663682.88090.qm@web31314.mail.mud.yahoo.com> Lee Luna had a great book that demonstrated artifacts. I refered to it frequently when running into technical issues. Histopathologic Methods and Color Atlas of Special Stains and Tissue Artifacts American Histolabs Inc. Publications Div. Kolb Center, 7605-F Airport Road, Gaitheresburg, MD 20879 Printed by johnson Printers, Downers Grove, IL Perhaps, calling the NSH office and ask them for details on how to obtain it. I purchased it directly from Lee, but I don't have the tele # now for american Histo labs. Good Luck! Akemi Allison-Tacha Special Stains Ma Ma --- "Orr, Rebecca" wrote: > Hi Everyone, > > I have heard there is a book that has pictures of > "histology artifacts" > > I'm thinking processing, cutting, staining > everything. > > I'd like to have it for our students. > > Thanks > > > > Becky Orr CLA,HT(ASCP)QIHC > > Assistant Manager, Anatomic Pathology > > Evanston Northwestern Healthcare > > 847-570-2763 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Wed Mar 28 11:10:26 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 28 11:10:35 2007 Subject: [Histonet] Artifact book In-Reply-To: Message-ID: <331164.6399.qm@web61213.mail.yahoo.com> Rebecca: You are probably referring to: Thompson & Luna: "An Atlas of Artifacts" published by Charles C. Thomas, Springfield IL 1978, xi + 190 pp It is well illustrated. Ren? J. "Orr, Rebecca" wrote: Hi Everyone, I have heard there is a book that has pictures of "histology artifacts" I'm thinking processing, cutting, staining everything. I'd like to have it for our students. Thanks Becky Orr CLA,HT(ASCP)QIHC Assistant Manager, Anatomic Pathology Evanston Northwestern Healthcare 847-570-2763 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- No need to miss a message. Get email on-the-go with Yahoo! Mail for Mobile. Get started. From oshel1pe <@t> cmich.edu Wed Mar 28 11:13:39 2007 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Wed Mar 28 11:14:02 2007 Subject: [Histonet] Artifact book In-Reply-To: <663682.88090.qm@web31314.mail.mud.yahoo.com> References: <663682.88090.qm@web31314.mail.mud.yahoo.com> Message-ID: $90 at half.com Phil >Lee Luna had a great book that demonstrated artifacts. > I refered to it frequently when running into >technical issues. > >Histopathologic Methods and Color Atlas of Special >Stains and Tissue Artifacts American Histolabs Inc. >Publications Div. Kolb Center, 7605-F Airport Road, >Gaitheresburg, MD 20879 Printed by johnson Printers, >Downers Grove, IL > >Perhaps, calling the NSH office and ask them for >details on how to obtain it. I purchased it directly >from Lee, but I don't have the tele # now for american >Histo labs. > >Good Luck! > >Akemi Allison-Tacha >Special Stains Ma Ma > >--- "Orr, Rebecca" wrote: > >> Hi Everyone, >> >> I have heard there is a book that has pictures of >> "histology artifacts" >> >> I'm thinking processing, cutting, staining >> everything. >> >> I'd like to have it for our students. >> >> Thanks >> >> >> >> Becky Orr CLA,HT(ASCP)QIHC >> >> Assistant Manager, Anatomic Pathology >> >> Evanston Northwestern Healthcare >> >> 847-570-2763 >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 voice: (989) 774-3576 dept. fax: (989) 774-3462 From TownsendD <@t> childrensdayton.org Wed Mar 28 11:13:22 2007 From: TownsendD <@t> childrensdayton.org (Dolores Townsend) Date: Wed Mar 28 11:14:08 2007 Subject: [Histonet] Artifact book Message-ID: Histopathologic Methods And Color Atlas Of Special Stains And Tissue Artifacts If you wonder where to find it, Amazon has a couple of copies of that book, half.com has one copy. Dolores >>> "Douglas D Deltour" 3/28/2007 12:34 PM >>> Histopathologic Methods And Color Atlas Of Special Stains And Tissue Artifacts by Lee G. Luna You can get it from... American Histolabs Inc 7605 Airpark Road Suite F, Gaithersburg, MD 20879 (866) 224-6298 Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Orr, Rebecca Sent: Wednesday, March 28, 2007 10:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Artifact book Hi Everyone, I have heard there is a book that has pictures of "histology artifacts" I'm thinking processing, cutting, staining everything. I'd like to have it for our students. Thanks Becky Orr CLA,HT(ASCP)QIHC Assistant Manager, Anatomic Pathology Evanston Northwestern Healthcare 847-570-2763 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Wed Mar 28 11:18:47 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Wed Mar 28 11:18:59 2007 Subject: [Histonet] Artifact book In-Reply-To: References: Message-ID: <9385DED5-C417-4B19-902D-A791C7D598DF@yahoo.com> Becky, I am looking at Lee's book right now and it has a significant number of images relating to artifacts. Akemi On Mar 28, 2007, at 10:12 AM, Orr, Rebecca wrote: > Hi Everyone, > > I have heard there is a book that has pictures of "histology > artifacts" > > I'm thinking processing, cutting, staining everything. > > I'd like to have it for our students. > > Thanks > > > > Becky Orr CLA,HT(ASCP)QIHC > > Assistant Manager, Anatomic Pathology > > Evanston Northwestern Healthcare > > 847-570-2763 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Wed Mar 28 11:28:12 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Mar 28 11:28:21 2007 Subject: AW: [Histonet] Artifact book In-Reply-To: Message-ID: <000a01c77156$14d79f70$6412a8c0@dielangs.at> Atlas of Microscopic Artifacts and Foreign Materials, by I-Tien Yeh 1997 I know it only by name. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Orr, Rebecca Gesendet: Mittwoch, 28. M?rz 2007 17:12 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Artifact book Hi Everyone, I have heard there is a book that has pictures of "histology artifacts" I'm thinking processing, cutting, staining everything. I'd like to have it for our students. Thanks Becky Orr CLA,HT(ASCP)QIHC Assistant Manager, Anatomic Pathology Evanston Northwestern Healthcare 847-570-2763 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TownsendD <@t> childrensdayton.org Wed Mar 28 11:28:47 2007 From: TownsendD <@t> childrensdayton.org (Dolores Townsend) Date: Wed Mar 28 11:29:40 2007 Subject: [Histonet] Book about Histology Artifacts Message-ID: Another book about Histology Artifacts: Atlas of Microscopic Artifacts and Foreign Materials (1997)By I-Tien Yeh, John S. J. Brooks, Giuseppe G. Pietra, and Rb4Dolores From gu.lang <@t> gmx.at Wed Mar 28 11:38:46 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Mar 28 11:38:56 2007 Subject: AW: [Histonet] Artifact book In-Reply-To: <20070328154100.20034gmx1@mx022.gmx.net> Message-ID: <000b01c77157$8edd26e0$6412a8c0@dielangs.at> Wanna have it ... There are so many good books only available in USA or much more expensive via German stores. Grmm. I've ordered it anyway (184 euro). Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Douglas D Deltour Gesendet: Mittwoch, 28. M?rz 2007 18:35 An: 'Orr, Rebecca'; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] Artifact book Histopathologic Methods And Color Atlas Of Special Stains And Tissue Artifacts by Lee G. Luna You can get it from... American Histolabs Inc 7605 Airpark Road Suite F, Gaithersburg, MD 20879 (866) 224-6298 Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Orr, Rebecca Sent: Wednesday, March 28, 2007 10:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Artifact book Hi Everyone, I have heard there is a book that has pictures of "histology artifacts" I'm thinking processing, cutting, staining everything. I'd like to have it for our students. Thanks Becky Orr CLA,HT(ASCP)QIHC Assistant Manager, Anatomic Pathology Evanston Northwestern Healthcare 847-570-2763 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pereirafamily <@t> nethere.com Wed Mar 28 13:00:06 2007 From: pereirafamily <@t> nethere.com (matt pereira) Date: Wed Mar 28 13:00:21 2007 Subject: [Histonet] jobs in arizona Message-ID: <000601c77162$ebd81890$c29a5545@pereirafarm> Hi all, Wanted to see if anyone could give me any insight as to jobs in Arizona. Planning on moving because California is too expensive! Any job in a hospital, veterinary or research setting. Thanks! From TJJ <@t> Stowers-Institute.org Wed Mar 28 14:56:23 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Wed Mar 28 14:56:49 2007 Subject: [Histonet] Re: background Message-ID: Pablo writes: >>Dear Histonetters, I am doing OMP immunohistochemistry in paraffin-embedded sections of sheep olfactory mucosa. In my positive control -mouse olfactory tissue- there is not any background, but in he sheep sections I get lot of unspecific labelling (vessels, connective tissue, respiratory mucosae). Being my primary antibody raised in goat I guess that there is an undesired cross reaction with the sheep tissue. Could I improve my blocking? I do half an hour in 2% BSA and 5% Horse normal serum. Should I use Goat normal serum? Thanks in advance for your input. Pablo Sanchez-Quinteiro Lugo (Spain)<< Luckily for you, Liz Chlipala covered this very topic in her absolutely wonderful NSH teleconference today. All you attendees out there, look at slide #48 and the answer is right there under her Antibody #2 example. One possibility for this is that your anti-goat secondary antibody shows minimal cross-reactivity with mouse, but considerable cross-reactivity with sheep. Check your antibody spec sheet and see if it's listed there. If not, call the company and ask them. Adding normal goat serum to a pre-primary antibody blocking step will most likely not have any affect if the problem is your secondary antibody. And you definitely want to stay far away from goat serum any time you are applying an anti-goat antibody to your tissues. You will get high background staining all over if you do that. Two possible fixes: Change to an anti-goat antibody that has been cross-adsorbed for sheep (or multiple species including sheep) and see if that fixes it. -or- Add 5-10% normal SHEEP serum to the diluent you use to make up your anti-goat antibody, and incubate your dilution in the tube prior to adding it to your sheep tissue. Thanks Liz! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From bethcoxx <@t> gmail.com Wed Mar 28 15:27:26 2007 From: bethcoxx <@t> gmail.com (Beth Cox) Date: Wed Mar 28 15:27:32 2007 Subject: [Histonet] RE: traveling histotech Message-ID: Hi Katie, I'm a traveling histotech, and have been doing this kind of work for almost 10 years (Wow! time sure flies!). I love working like this. I like the flexibility. I make enough money when I'm on an assignment to be able to take a little time off between assignments. I like the variety. I'm never anywhere so long that the politics or the personalities start getting to me. I learn something new everywhere I go, and I'm constantly gaining new skills. I stay at an assignment long enough to really see the area of the country. (average length of assignment is 13 weeks). I work for a national agency, but I also take some assignments on my own. Most assignments are really good and I've met some great people. Most places are welcoming and friendly. Naturally, there have been a couple bad apples in the bunch, but, after 13 weeks, I move on. I am able to turn down any assignment that they offer, if I choose to. If you are seriously considering this kind of work, I'd be happy to elaborate more on things to consider when choosing the agency to work with. Or if you have specific questions, feel free to contact me. Beth Cox ------------------------------ Message: 6 Date: Tue, 27 Mar 2007 18:07:59 -0600 From: "katie holmberg" Subject: [Histonet] traveling histotech To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1; format=flowed Does anyone have any experience or insight as a traveling histotech? Although this isn't a technical question, I would greatly appreciate any input. From scoop <@t> mail.nih.gov Wed Mar 28 15:53:06 2007 From: scoop <@t> mail.nih.gov (scoop@mail.nih.gov) Date: Wed Mar 28 15:53:11 2007 Subject: [Histonet] thanks for the help with mouse bone marrow fixation Message-ID: Dear All, Thanks very much for the good advice re improving my bone marrow fixation. I broke the femurs in the center as suggested (I wanted the ends) and also tried to fix a little pelvis and some lumbar vertebrae. I put the bones in a 50 ml tube half full of NBF and rotated end over end in the cold overnight (as suggested). I didn't want to use the fixative/decal with EDTA because I was afraid of losing all the iron in the bone marrow so I decaled quickly the next day. I got great bone marrows with only a few tiny areas of brown mush (I think those are the areas that the fixative didn't reach well) as opposed to the brown mush with a few tiny areas of well fixed marrow that I got in the past. I did this as a favor for a coworker. Now my only problem is that they want me to do many more (the bone marrows confirmed the opposite of the result they had hoped for). Next step - teaching someone else how to do it. Anyway, thanks very much for the great advice. Sharon From Allison_Scott <@t> hchd.tmc.edu Wed Mar 28 16:07:49 2007 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Wed Mar 28 16:07:56 2007 Subject: [Histonet] Where's the histonet Message-ID: <1872B4A455B7974391609AD8034C79FC067129@LBEXCH01.hchd.local> I have not received the histonet in a couple of days. What's happening? Allison Scott CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From AGrobe2555 <@t> aol.com Wed Mar 28 17:11:03 2007 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Wed Mar 28 17:11:15 2007 Subject: [Histonet] Sheep TIMPs Message-ID: Good Afternoon, I was wondering if there is anyone who could suggest some anti-sheep TIMP 1, 2 and 3 antibodies that will work for paraffin-embedded tissues. After spending the last few hours looking, I have found some that were published in the literature, but the provenance was vague at best. Thanks, Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital ************************************** See what's free at http://www.aol.com. From mtarango <@t> nvcancer.org Wed Mar 28 18:56:12 2007 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Wed Mar 28 18:56:20 2007 Subject: [Histonet] delivery time of last tray of H&E -FIXATION In-Reply-To: <005b01c76da1$378f5110$6a9a9618@Katri> Message-ID: <5AEC610C1CE02945BD63A395BA763EDE011B6C59@NVCIEXCH02.NVCI.org> Can't histonetters stick together and enforce proper fixation? We could start a campaign with a clever slogan, "Force Fix!" maybe. Get NSH to sponsor us and make buttons or something. It can be the histo theme for 2008. We could have informational flyers explaining to pathologists/dermatologists/whomever that getting the specimen wet with formalin doesn't quite ensure proper fixation. I mean, seriously though, it is a BIG problem. In each lab I've worked, I've seen tons of problems that could have been prevented by simply fixing the tissue adequately. Who is going to do anything about it but us? Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 Mobile (702) 759-9229 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Katri Tuomala Sent: Friday, March 23, 2007 4:16 PM To: gu.lang@gmx.at; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] delivery time of last tray of H&E I totally agree with you Gudrun, I think too many samples get inadequate fixation and processing compromising the patients' samples. There has been an improvement in our lab in regards to larger specimens. Most of them are now opened up, fixed overnight and processed in an "extended program" in VIP. What a joy to cut them, no more fatty holes to hand in! However many biopsies still don't get adequate fixation, particularly if IHC is requested. Katri Katri Tuomala, Hamilton, Ontario ----- Original Message ----- From: "Gudrun Lang" To: Sent: Friday, March 23, 2007 11:43 AM Subject: AW: [Histonet] delivery time of last tray of H&E And then ... the patient has to get an appointment for the talk about the report with his/her doctor or has to get a date for the necessary surgery. In most cases there is no reason for such short TATs. Why all this hurry? The specimen aren't properly fixed, people have to work in nightshifts; There is no tumor, that grows in such a speed ... My point of view. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Cheri Miller Gesendet: Freitag, 23. M?rz 2007 14:44 An: 'Marshall Terry Dr,Consultant Histopathologist'; 'Akemi Allison-Tacha'; 'Rene J Buesa'; 'Galiotto, Laura'; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] delivery time of last tray of H&E Turn around time Terry. For us it's about getting the slides to the Pathologist early so they may order whatever additional testing needed and still get a hard copy report to the Clinician by the end of the day. We have 24 hour turnaround for most cases. Cheri Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Thursday, March 22, 2007 12:28 PM To: Akemi Allison-Tacha; Rene J Buesa; Galiotto, Laura; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] delivery time of last tray of H&E This is all bloody unbelievable. Don't any of you sleep? What is the point of working at night? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: 22 March 2007 17:25 To: Rene J Buesa; Galiotto, Laura; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] delivery time of last tray of H&E Ren?--That was similar to the protocol I followed in the old days before 24/6 . We provided rush's 1st, followed by bx's by 8AM, we did however, provide special stains and recuts and IHC's by 2-3 PM, depending if it wasn't a lengthy procedure. If you have off site facilities to provide for, that is a different situation. You have to work around courier sheduals for delivering to off site facilities. Everyone needs their slides and time is money!! With the new microwave processing technology that is now in use, there has been a shift to having the majority of staff starting at 10 PM at night. MUCH TO THE DISLIKING OF HISTOTECH"S. The day shift is minimum. IHC is done during the day shift, after the pathologist has determined to do so. In most situations, a battery of IHC's tests are pre-set, as well as with special stains. Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting & Staffing Specializing in Histology, SS, IHC, & Microarray Madison, WI Tele: (925) 788-0900 E-Mail: akemiat3377@yahoo.com --- Rene J Buesa wrote: > Laura: > For a laboratory I used to manage with quite similar annual workload > we had the following > schedule: > 1- last tissue accepted at 7:00 PM; > 2- two VIPs atarting with delay to be finished at 4:00 AM next day; > 3- Rushes ready for the pathologists at 8:00 AM next day; > 4-All slides ready, the latest, at 1:00 PM. > 5- All IHC ready by 2 PM > 6- all HC ready by noon. > Ren? J. > > "Galiotto, Laura" wrote: > Hello Fellow Histo Tech's, > Will anyone be willing to share the delivery time of the last tray of > H&E for the days workload to the pathologist reading them? > What is your cutoff time for specimen acceptance? > What is your annual case workload? > I am trying to improve the TAT from specimen gross to the H&E > delivered to the pathologist. Unless we change the cutoff time I can > not see how I can do this? Any suggestions? > We process 22,000 cases a year an average of 280 blks a day consisting > of types of tissue. > Please Help > Laura > > > ******************* PLEASE NOTE ******************* This > E-Mail/telefax message and any documents accompanying this > transmission may contain information that is privileged, confidential, > and/or exempt from disclosure under applicable law and is intended > solely for the addressee(s) named above. If you are not the intended > addressee/recipient, you are hereby notified that any use of, > disclosure, copying, distribution, or reliance on the contents of this > E-Mail/telefax information is strictly prohibited and may result in > legal action against you. Please reply to the sender advising of the > error in transmission and immediately delete/destroy the message and > any accompanying documents. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Sucker-punch spam with award-winning protection. > Try the free Yahoo! Mail Beta. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From katri <@t> cogeco.ca Wed Mar 28 23:04:59 2007 From: katri <@t> cogeco.ca (Katri Tuomala) Date: Wed Mar 28 23:05:04 2007 Subject: [Histonet] delivery time of last tray of H&E -FIXATION References: <5AEC610C1CE02945BD63A395BA763EDE011B6C59@NVCIEXCH02.NVCI.org> Message-ID: <006701c771b7$6baaad90$6a9a9618@Katri> Mark, The constant argument from pathologists is the all important TAT (turn around time). Fortunately the message is slowly getting through due to problems arising with immunohistocemistry and inadequate fixation. Fixation recommendations for Her2 testing is a step in the right direction and lymphoma protocols (fix one piece in NBF minimum overnight) in our lab has been in place for some years now. So, there's hope, but you are right, histotechs have to keep getting the message through to people, who make policy changes. It seems that current TAT is not there to serve the patient, but some other entity unknown to me. Just think, if it were your specimen, surely you could wait an extra day for a proper diagnosis. Just my opinion. Katri Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "Tarango, Mark" To: "Katri Tuomala" ; ; Sent: Wednesday, March 28, 2007 7:56 PM Subject: RE: [Histonet] delivery time of last tray of H&E -FIXATION Can't histonetters stick together and enforce proper fixation? We could start a campaign with a clever slogan, "Force Fix!" maybe. Get NSH to sponsor us and make buttons or something. It can be the histo theme for 2008. We could have informational flyers explaining to pathologists/dermatologists/whomever that getting the specimen wet with formalin doesn't quite ensure proper fixation. I mean, seriously though, it is a BIG problem. In each lab I've worked, I've seen tons of problems that could have been prevented by simply fixing the tissue adequately. Who is going to do anything about it but us? Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 Mobile (702) 759-9229 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Katri Tuomala Sent: Friday, March 23, 2007 4:16 PM To: gu.lang@gmx.at; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] delivery time of last tray of H&E I totally agree with you Gudrun, I think too many samples get inadequate fixation and processing compromising the patients' samples. There has been an improvement in our lab in regards to larger specimens. Most of them are now opened up, fixed overnight and processed in an "extended program" in VIP. What a joy to cut them, no more fatty holes to hand in! However many biopsies still don't get adequate fixation, particularly if IHC is requested. Katri Katri Tuomala, Hamilton, Ontario ----- Original Message ----- From: "Gudrun Lang" To: Sent: Friday, March 23, 2007 11:43 AM Subject: AW: [Histonet] delivery time of last tray of H&E And then ... the patient has to get an appointment for the talk about the report with his/her doctor or has to get a date for the necessary surgery. In most cases there is no reason for such short TATs. Why all this hurry? The specimen aren't properly fixed, people have to work in nightshifts; There is no tumor, that grows in such a speed ... My point of view. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Cheri Miller Gesendet: Freitag, 23. M?rz 2007 14:44 An: 'Marshall Terry Dr,Consultant Histopathologist'; 'Akemi Allison-Tacha'; 'Rene J Buesa'; 'Galiotto, Laura'; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] delivery time of last tray of H&E Turn around time Terry. For us it's about getting the slides to the Pathologist early so they may order whatever additional testing needed and still get a hard copy report to the Clinician by the end of the day. We have 24 hour turnaround for most cases. Cheri Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Thursday, March 22, 2007 12:28 PM To: Akemi Allison-Tacha; Rene J Buesa; Galiotto, Laura; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] delivery time of last tray of H&E This is all bloody unbelievable. Don't any of you sleep? What is the point of working at night? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: 22 March 2007 17:25 To: Rene J Buesa; Galiotto, Laura; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] delivery time of last tray of H&E Ren?--That was similar to the protocol I followed in the old days before 24/6 . We provided rush's 1st, followed by bx's by 8AM, we did however, provide special stains and recuts and IHC's by 2-3 PM, depending if it wasn't a lengthy procedure. If you have off site facilities to provide for, that is a different situation. You have to work around courier sheduals for delivering to off site facilities. Everyone needs their slides and time is money!! With the new microwave processing technology that is now in use, there has been a shift to having the majority of staff starting at 10 PM at night. MUCH TO THE DISLIKING OF HISTOTECH"S. The day shift is minimum. IHC is done during the day shift, after the pathologist has determined to do so. In most situations, a battery of IHC's tests are pre-set, as well as with special stains. Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting & Staffing Specializing in Histology, SS, IHC, & Microarray Madison, WI Tele: (925) 788-0900 E-Mail: akemiat3377@yahoo.com --- Rene J Buesa wrote: > Laura: > For a laboratory I used to manage with quite similar annual workload > we had the following > schedule: > 1- last tissue accepted at 7:00 PM; > 2- two VIPs atarting with delay to be finished at 4:00 AM next day; > 3- Rushes ready for the pathologists at 8:00 AM next day; > 4-All slides ready, the latest, at 1:00 PM. > 5- All IHC ready by 2 PM > 6- all HC ready by noon. > Ren? J. > > "Galiotto, Laura" wrote: > Hello Fellow Histo Tech's, > Will anyone be willing to share the delivery time of the last tray of > H&E for the days workload to the pathologist reading them? > What is your cutoff time for specimen acceptance? > What is your annual case workload? > I am trying to improve the TAT from specimen gross to the H&E > delivered to the pathologist. Unless we change the cutoff time I can > not see how I can do this? Any suggestions? > We process 22,000 cases a year an average of 280 blks a day consisting > of types of tissue. > Please Help > Laura > > > ******************* PLEASE NOTE ******************* This > E-Mail/telefax message and any documents accompanying this > transmission may contain information that is privileged, confidential, > and/or exempt from disclosure under applicable law and is intended > solely for the addressee(s) named above. If you are not the intended > addressee/recipient, you are hereby notified that any use of, > disclosure, copying, distribution, or reliance on the contents of this > E-Mail/telefax information is strictly prohibited and may result in > legal action against you. Please reply to the sender advising of the > error in transmission and immediately delete/destroy the message and > any accompanying documents. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Sucker-punch spam with award-winning protection. > Try the free Yahoo! Mail Beta. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From michael.owen <@t> fda.hhs.gov Thu Mar 29 09:51:37 2007 From: michael.owen <@t> fda.hhs.gov (Owen, Michael P) Date: Thu Mar 29 09:51:54 2007 Subject: [Histonet] Safety Survey In-Reply-To: <44843.99045.qm@web61216.mail.yahoo.com> Message-ID: <15B0433665D1724BAE49F6E4476AD81C4D1108@FMD3VS012.fda.gov> Dear List Members, CDC released the new edition of the BMBL, one of the standard references for biosafety, last month. Two other useful resources are the Third Edition of the WHO Laboratory Biosafety Manual released in December 2004 and the ABSA Web site. CDC: Biosafety in Microbiological and Biomedical Laboratories (BMBL) Fifth Edition http://www.cdc.gov/od/ohs/biosfty/bmbl5/bmbl5toc.htm WHO Publications: Laboratory Biosafety Manual Third Edition http://www.who.int/csr/resources/publications/csrpublications/en/index2. html http://www.who.int/csr/resources/publications/biosafety/WHO_CDS_CSR_LYO_ 2004_11/en/index.html American Biological Safety Association (ABSA) http://www.absa.org Sincerely, Michael Michael P. Owen, Regulatory Microbiologist U.S. FDA Pacific Regional Lab Northwest 22201 23rd Drive SE Bothell, WA 98021-4421 Phone: 425-483-4865 E-Mail: michael.owen@fda.hhs.gov From psanquin <@t> lugo.usc.es Thu Mar 29 10:18:12 2007 From: psanquin <@t> lugo.usc.es (Pablo =?iso-8859-1?Q?S=E1nchez?= Quinteiro) Date: Thu Mar 29 10:18:17 2007 Subject: [Histonet] Re: background In-Reply-To: Message-ID: <3.0.6.32.20070329171812.00d1dae8@pop.lugo.usc.es> Histonetters; Lots of thanks for your helpful answers to my question. I am really grateful. Pablo Sanchez-Quinteiro Department of Anatomy Faculty of Veterinary Sciences Lugo (Spain) From EWURDAK <@t> CSBSJU.EDU Thu Mar 29 11:51:46 2007 From: EWURDAK <@t> CSBSJU.EDU (Wurdak, Elizabeth) Date: Thu Mar 29 11:51:53 2007 Subject: [Histonet] Harris substitute Message-ID: Hello Histonetters, I would like to thank all who generously shared their expertise with regards to my request for information on a mercury-free substitute for Harris hematoxylin. We decided to try Protocol. Liz Elizabeth Wurdak, PhD Biology Department Saint John?s University Collegeville, MN 56321 Tel: (320) 363-3177 From hej01 <@t> health.state.ny.us Thu Mar 29 14:39:01 2007 From: hej01 <@t> health.state.ny.us (Helen E Johnson) Date: Thu Mar 29 14:39:11 2007 Subject: [Histonet] paraformaldehyde Message-ID: Hi Histonetters, Can you please tell me which paraformaldehyde is used for frozen sections and its preparation. Helen Johnson (hej01@health.state.ny.us) From SecrestK <@t> wvuh.com Thu Mar 29 15:16:27 2007 From: SecrestK <@t> wvuh.com (Secrest, Kimberly) Date: Thu Mar 29 15:16:44 2007 Subject: [Histonet] KIT Mutations Message-ID: <7708E0A8E46BDC40B23113F97FB0FB23031DCF2B@nt-exchange1.wvuh.wvuhs.com> Hi all, I have a surgeon requesting testing for exon 9 and 11 mutation analysis of a metastatic tumor suspected of GIST origin. I know that the CD-117 antibody targets that "area"(exon 9,11,13,17) , but I need to have the tissue tested for specific exon mutations. Does anyone know of a lab that does this testing. I've become exasperated searching the internet. I appreciate any suggestions! Kimberly Secrest HTL, QIHC Histology Technical Specialist West Virginia University Hospital From LGeuss <@t> histogenics.com Thu Mar 29 15:54:39 2007 From: LGeuss <@t> histogenics.com (Laura Geuss) Date: Thu Mar 29 15:54:44 2007 Subject: [Histonet] Qtracker labeling Message-ID: <520E4D4FB8A9E545ACB770E37D20E3DA1354CB@histoex01.histogenics.local> Hi all, I am looking to do some work with Invitrogen Qtracker nanocrystals. Has anyone had any experience processing and paraffin embedding samples which have been incubated with Qtracker? Are they affected by the process? Thanks for the help! Laura Geuss Histogenics, Corp. Research & Development Waltham, MA 02451 From rod.coombe <@t> imvs.sa.gov.au Thu Mar 29 19:24:39 2007 From: rod.coombe <@t> imvs.sa.gov.au (Rod Coombe) Date: Thu Mar 29 19:24:52 2007 Subject: [Histonet] IPA Message-ID: <002201c77261$cde74b30$d86d140a@ITP36079> Hello all, I would be interested in feedback on people's experience in processing with IPA instead of xylene. If you are using it, do you find it quicker and does it process fatty blocks (assuming they are properly fixed and cut) adequately. Thankyou Rod Coombe Manager Tissue Pathology IMVS Frome Road, Adelaide South Australia Phone 61 8 8222 3201 Fax 61 8 8222 3204 From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Mar 30 05:18:23 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Mar 30 05:18:30 2007 Subject: [Histonet] John Kerr Message-ID: <86ADE4EB583CE64799A9924684A0FBBF012474A5@wahtntex2.waht.swest.nhs.uk> I've lost your abstract concerning apoptosis, sodium pumps and fixation. Could you send it to me again? Maybe to Kemlo@f2s.com? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; In a world where change is inevitable and continuous, the need to achieve that change without violence is essential for survival. --Andrew Young This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From bakevictoria <@t> gmail.com Fri Mar 30 06:16:40 2007 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Fri Mar 30 06:16:49 2007 Subject: [Histonet] Paper Bag In-Reply-To: <460a5db8.00375f3f.0f81.ffff896dSMTPIN_ADDED@mx.google.com> References: <460a5db8.00375f3f.0f81.ffff896dSMTPIN_ADDED@mx.google.com> Message-ID: <4f016b690703300416j7d7e4c7cp7428317a70251c4e@mail.gmail.com> Douglas, I have also been looking to find the paper biopsy bags, also known as "tea bags". The fellow who had gotten this product on the market passed away not that long ago. What I'm gathering from various vendors is that they are not manufacturing these bags due to some legal ranglings. At this point the only item we've been able to look at that even comes close to what we need comes from Surgipath. It's a tinted sheet that we use for our most delicate biopsies. I'm at home now so I don't have the catalog number, but if you want I would contact Surgipath directly I'm sure they can help you. It's a subsitute, not what you're looking for but it will hold up. Vikki Baker Histology Supervisor NIH/NCI/LP, Bethesda Maryland On 3/28/07, Douglas D Deltour wrote: > Hello All, > > > > I am looking for a supplier of the paper biopsy bags. Most of the sources > that I use have discontinued the paper for the nylon bags. > > > > Thanks. > > > > > > Douglas D. Deltour HT(ASCP) > > Histology Manager > > Professional Pathology Services, PC > > One Science Court > > Suite 200 > > Columbia, SC 29203 > > (803)252-1913 > > Fax (803)254-3262 > > > > ***************************************************** > > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of the individual or entity to > which it is addressed and may contain information that is privileged, > confidential and exempt from disclosure under applicable law. If the reader > of this message is not the intended recipient, you are hereby notified that > any dissemination, distribution, or copying of this communication is > strictly prohibited by law. If you have received this communication in > error, please notify me immediately. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From sonya.martin <@t> soton.ac.uk Fri Mar 30 06:48:20 2007 From: sonya.martin <@t> soton.ac.uk (Martin S.) Date: Fri Mar 30 06:49:33 2007 Subject: [Histonet] Posting images? Message-ID: <71437982F5B13A4D9A5B2669BDB89EE4023E3565@ISS-CL-EX-V1.soton.ac.uk> Hi there, Does anyone know of a site out there where we can post microscopy images? I have an H&E stained image that I want to submit to our local micrograph competition. It is of some unknown structure that appeared in a paraffin embedded mouse kidney sample. The competition is based on artistic merit rather than scientific but I'd like to know if anyone has any clues as to what the structure might be! Thanks Sonya From pmcardle <@t> ebsciences.com Fri Mar 30 07:41:02 2007 From: pmcardle <@t> ebsciences.com (Phil McArdle) Date: Fri Mar 30 07:41:12 2007 Subject: [Histonet] IPA In-Reply-To: <002201c77261$cde74b30$d86d140a@ITP36079> References: <002201c77261$cde74b30$d86d140a@ITP36079> Message-ID: <460D055E.7080300@ebsciences.com> Hello: I am with a vendor of laboratory microwave processors, and have some input re: IPA (2-propanol) in a microwave processing context, for those who are interested. Best regards, Phil McArdle -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Mobile: 860.597.6796 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it, and it was probably my mistake anyway. So please do the right thing and make this e-mail go away. Thank you. Best regards, Phil McArdle Rod Coombe wrote: > Hello all, > > > > I would be interested in feedback on people's experience in processing with > IPA instead of xylene. If you are using it, do you find it quicker and does > it process fatty blocks (assuming they are properly fixed and cut) > adequately. > > > > Thankyou > > > > Rod Coombe > > Manager > > Tissue Pathology > > IMVS > > Frome Road, Adelaide > > South Australia > > Phone 61 8 8222 3201 > > Fax 61 8 8222 3204 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Mobile: 860.597.6796 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it, and it was probably my mistake anyway. So please do the right thing and make this e-mail go away. Thank you. From Jackie.O'Connor <@t> abbott.com Fri Mar 30 07:56:27 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Mar 30 07:56:58 2007 Subject: [Histonet] Murine brain perfusion In-Reply-To: <032820070109.22942.4609C066000A0E8E0000599E22070032019D09020704040A0105@comcast.net> Message-ID: How do you do it? We would like to perfuse whole mouse brains with fixative (what kind?) in order to make 2mm coronal sections to visually examine the brain for therapeutic changes as soon after harvest as possible. Would someone be willing to walk me through their protocol? I don't have any experience with this at all - and they're looking to me for answers. Happy Friday - Jackie From stamptrain <@t> yahoo.com Fri Mar 30 08:19:07 2007 From: stamptrain <@t> yahoo.com (Roger Moretz) Date: Fri Mar 30 08:19:15 2007 Subject: [Histonet] Murine brain perfusion In-Reply-To: Message-ID: <540963.99137.qm@web55804.mail.re3.yahoo.com> It has been years, but here goes. We used a 2% paraform+1% glut mixture in a 0.1M buffer (a Sorensen's phosphate was our choice) at about pH 7.2. The setup was fairly simple: a drip bottle was filled with warm (actually RT) fixative and elevated to about 6ft. A second bottle held saline for doing a flush and the 2 bottles were connected via a simple 2-way valve. A 26ga needle was inserted into the ascending aorta and the abdominal aorta was cut to allow outflow. The flush is run until clear (probably no more than 30-60sec) at which point the valve is switched to the fixative. Fixation is adequate after about 5min (max). The brain is removed and placed in fresh fixative for at least 1hr. (This technique should actually give whole body fixation.) Coronal sections were taken of the brain and either processed for electron microscopy, into paraffin for routine H&E, placed on a vibratome and 50um sections obtained for tracer localization (evaluating blood-brain barrier integrity), etc. Probably the diciest part of the technique is inserting the cannula correctly into the ascending aorta and clamping it in place--we used small vascular clamps for this. Another life, many years ago. Roger Moretz, Ph.D. Dept. of Toxicology Boehringer Ingelheim Pharmaceuticals, Inc. Ridgefield, CT --- Jackie M O'Connor wrote: > How do you do it? We would like to perfuse whole > mouse brains with > fixative (what kind?) in order to make 2mm coronal > sections to visually > examine the brain for therapeutic changes as soon > after harvest as > possible. Would someone be willing to walk me > through their protocol? > I don't have any experience with this at all - and > they're looking to me > for answers. > Happy Friday - Jackie > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ____________________________________________________________________________________ 8:00? 8:25? 8:40? Find a flick in no time with the Yahoo! Search movie showtime shortcut. http://tools.search.yahoo.com/shortcuts/#news From tp2 <@t> medicine.wisc.edu Fri Mar 30 09:10:17 2007 From: tp2 <@t> medicine.wisc.edu (Thomas Pier) Date: Fri Mar 30 09:10:48 2007 Subject: [Histonet] IPA Message-ID: <460CD3F9020000DF00006251@gwmail.medicine.wisc.edu> Since it's Friday I thought that IPA was referring to India Pale Ale. I guess not. >>> "Rod Coombe" 03/29/07 7:24 PM >>> Hello all, I would be interested in feedback on people's experience in processing with IPA instead of xylene. If you are using it, do you find it quicker and does it process fatty blocks (assuming they are properly fixed and cut) adequately. Thankyou Rod Coombe Manager Tissue Pathology IMVS Frome Road, Adelaide South Australia Phone 61 8 8222 3201 Fax 61 8 8222 3204 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From angela.mcnabola <@t> ikonisys.com Fri Mar 30 09:11:11 2007 From: angela.mcnabola <@t> ikonisys.com (Angela McNabola) Date: Fri Mar 30 09:11:20 2007 Subject: [Histonet] Help with FISH Message-ID: <4ECD18F12E443644B1F3C924A2039824134D65@ikoexchange.ikonisys.com> Hi all, I just started a new job (after Bayer here in CT decided to give 400+ researchers the boot) and shut its doors. I need help with FISH. I am familiar with the techniques and background methods, however, the person who was responsible for the probe work-up and validation is leaving shortly. I can "perform" the procedure using purchased probes with good results, but I really need some training. Can anyone make any recommendations as to where we can go, who we can talk to, and some good reference materials that could help us? I should mention that I am on the east coast, so any travel for training would have to be on this end of the country. I should also say that I do have a good foundation of molecular biology and pathology, but really need some advice. Thank you in advance, Angela Angela McNabola, MS HT(ASCP)SLS, QIHC Research Scientist Ikonisys, Inc. 5 Science Park New Haven, CT 06511 203-776-0791 angela.mcnabola@ikonisys.com From oshel1pe <@t> cmich.edu Fri Mar 30 09:30:17 2007 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Fri Mar 30 09:30:34 2007 Subject: [Histonet] IPA In-Reply-To: <460CD3F9020000DF00006251@gwmail.medicine.wisc.edu> References: <460CD3F9020000DF00006251@gwmail.medicine.wisc.edu> Message-ID: Well, I'd rather "process" some India Pale Ale instead of xylene, 'though I'm more of a Stout man. Make whatever pun you wish about that ... Phil >Since it's Friday I thought that IPA was referring to India Pale Ale. I >guess not. > >>>> "Rod Coombe" 03/29/07 7:24 PM >>> >Hello all, > > > >I would be interested in feedback on people's experience in processing >with >IPA instead of xylene. If you are using it, do you find it quicker and >does >it process fatty blocks (assuming they are properly fixed and cut) >adequately. > > > >Thankyou > > > >Rod Coombe > >Manager > >Tissue Pathology > >IMVS > >Frome Road, Adelaide > >South Australia > >Phone 61 8 8222 3201 > >Fax 61 8 8222 3204 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 voice: (989) 774-3576 dept. fax: (989) 774-3462 From jfish <@t> gladstone.ucsf.edu Fri Mar 30 09:50:09 2007 From: jfish <@t> gladstone.ucsf.edu (Jo Dee Fish) Date: Fri Mar 30 09:50:24 2007 Subject: [Histonet] Murine brain perfusion In-Reply-To: <540963.99137.qm@web55804.mail.re3.yahoo.com> Message-ID: <001101c772da$b6a59390$8903010a@JFISH> Dear Histonetters, Roger and Jackie, I follow about the same protocol on mice, but with one major difference. In order to keep the heart beating (facilitates good perfusion, and best fixation), cut the posterior vena cava rather than the ascending aorta. If you cut the aorta you will disrupt the closed vascular system and the fixative won't be able to reach the organs and brain. The whole purpose of perfusion is to get the fixative into the deeper tissues, so you want to keep the vascular circuit closed so the fixative can be pumped through the entire body, but if you cut the aorta you will only be perfusing for a very short distance. The posterior vena cava is a darker purplish blue and the aorta will be paler, DON'T cut the pale one, cut the purple one! You should do well with this. One other thing I do differently is I use an 18 gauge canula and insert it into the left ventricle of the heart, it gives you a bigger target, is easier to hold, and you don't have to spend as much time "finding" the aorta and clamping it in place. You can hold the canula in place while switching the valve from saline buffer to fixative (may take a little practice, but is easily learned). Good luck, Jo Dee Jo Dee Fish Research Technologist III Gladstone Institute of Cardiovascular Disease Telephone: (415) 734-2567 Fax: (415) 355-0824 E-mail: jfish@gladstone.ucsf.edu Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roger Moretz Sent: Friday, March 30, 2007 6:19 AM To: Jackie M O'Connor; histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Murine brain perfusion It has been years, but here goes. We used a 2% paraform+1% glut mixture in a 0.1M buffer (a Sorensen's phosphate was our choice) at about pH 7.2. The setup was fairly simple: a drip bottle was filled with warm (actually RT) fixative and elevated to about 6ft. A second bottle held saline for doing a flush and the 2 bottles were connected via a simple 2-way valve. A 26ga needle was inserted into the ascending aorta and the abdominal aorta was cut to allow outflow. The flush is run until clear (probably no more than 30-60sec) at which point the valve is switched to the fixative. Fixation is adequate after about 5min (max). The brain is removed and placed in fresh fixative for at least 1hr. (This technique should actually give whole body fixation.) Coronal sections were taken of the brain and either processed for electron microscopy, into paraffin for routine H&E, placed on a vibratome and 50um sections obtained for tracer localization (evaluating blood-brain barrier integrity), etc. Probably the diciest part of the technique is inserting the cannula correctly into the ascending aorta and clamping it in place--we used small vascular clamps for this. Another life, many years ago. Roger Moretz, Ph.D. Dept. of Toxicology Boehringer Ingelheim Pharmaceuticals, Inc. Ridgefield, CT --- Jackie M O'Connor wrote: > How do you do it? We would like to perfuse whole > mouse brains with > fixative (what kind?) in order to make 2mm coronal sections to > visually examine the brain for therapeutic changes as soon after > harvest as > possible. Would someone be willing to walk me > through their protocol? > I don't have any experience with this at all - and they're looking to > me for answers. > Happy Friday - Jackie > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ____________________________________________________________________________ ________ 8:00? 8:25? 8:40? Find a flick in no time with the Yahoo! Search movie showtime shortcut. http://tools.search.yahoo.com/shortcuts/#news _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CCross6032 <@t> aol.com Fri Mar 30 09:56:23 2007 From: CCross6032 <@t> aol.com (CCross6032@aol.com) Date: Fri Mar 30 09:56:38 2007 Subject: [Histonet] Murine brain perfusion Message-ID: hi everyone - the only other thing i would add is, unless you are going to do immunohistochemistry and are worried about epitopes falling off in the fix, would be to perfuse as everyone has stated and then decapitate, peel the skin over the skull back, remove the top of the calvarium, and let the brain sit in the calvarium overnight in the fixative you have chosen (i usually use 10% NBF, but i do paraffin embedded histo). this really gives a nice fix to the neurons before removing from the calvarium. I have done it both ways, and feel like there are much fewer compressed neurons (that can mimic or obscure neuronal necrosis) if you let it really fix before you touch it. hope this helps! Cheryl ************************************** See what's free at http://www.aol.com. From PMonfils <@t> Lifespan.org Fri Mar 30 10:03:29 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Mar 30 10:03:38 2007 Subject: [Histonet] Posting images? In-Reply-To: <71437982F5B13A4D9A5B2669BDB89EE4023E3565@ISS-CL-EX-V1.soton.ac.uk> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273C5C@LSRIEXCH1.lsmaster.lifespan.org> There are a number of sites where images of any kind can be posted free of charge. I don't have much experience with them, as I always post images to my personal webspace provided by my internet provider. Are you sure you don't have such personal webspace available? In any case, one such site that I see used a lot is www.badongo.com From scoop <@t> mail.nih.gov Fri Mar 30 10:35:45 2007 From: scoop <@t> mail.nih.gov (scoop@mail.nih.gov) Date: Fri Mar 30 10:35:58 2007 Subject: [Histonet] Murine brain perfusion In-Reply-To: <540963.99137.qm@web55804.mail.re3.yahoo.com> References: <540963.99137.qm@web55804.mail.re3.yahoo.com> Message-ID: Dear Histonetters, I do mouse perfusion using a 24 gauge tubing adaptor (no sharp ends) in the left ventricle and cut the right atrium open (you need somewhere for the perfusion fluid to exit the sytem). But I have always wanted to try cannulating the aorta or vena cava - what size catheter or tubing do people use for that? Also, is it possible to cannulate the aorta through the left ventricle? Thanks, Sharon >It has been years, but here goes. We used a 2% >paraform+1% glut mixture in a 0.1M buffer (a >Sorensen's phosphate was our choice) at about pH 7.2. >The setup was fairly simple: a drip bottle was filled >with warm (actually RT) fixative and elevated to about >6ft. A second bottle held saline for doing a flush >and the 2 bottles were connected via a simple 2-way >valve. A 26ga needle was inserted into the ascending >aorta and the abdominal aorta was cut to allow >outflow. The flush is run until clear (probably no >more than 30-60sec) at which point the valve is >switched to the fixative. Fixation is adequate after >about 5min (max). The brain is removed and placed in >fresh fixative for at least 1hr. (This technique >should actually give whole body fixation.) Coronal >sections were taken of the brain and either processed >for electron microscopy, into paraffin for routine >H&E, placed on a vibratome and 50um sections obtained >for tracer localization (evaluating blood-brain >barrier integrity), etc. Probably the diciest part of >the technique is inserting the cannula correctly into >the ascending aorta and clamping it in place--we used >small vascular clamps for this. > >Another life, many years ago. > >Roger Moretz, Ph.D. >Dept. of Toxicology >Boehringer Ingelheim Pharmaceuticals, Inc. >Ridgefield, CT > >--- Jackie M O'Connor >wrote: > >> How do you do it? We would like to perfuse whole >> mouse brains with >> fixative (what kind?) in order to make 2mm coronal >> sections to visually >> examine the brain for therapeutic changes as soon >> after harvest as >> possible. Would someone be willing to walk me >> through their protocol? >> I don't have any experience with this at all - and >> they're looking to me >> for answers. >> Happy Friday - Jackie >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > >____________________________________________________________________________________ >8:00? 8:25? 8:40? Find a flick in no time >with the Yahoo! Search movie showtime shortcut. >http://tools.search.yahoo.com/shortcuts/#news > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Robert.Lott <@t> TriadHospitals.com Fri Mar 30 10:48:21 2007 From: Robert.Lott <@t> TriadHospitals.com (Lott, Robert) Date: Fri Mar 30 10:48:30 2007 Subject: [Histonet] Retail Price of the Prisma Stainer/Coverslipper Message-ID: <673832E27C45FC4D97EF758FFC777C2701E68D61@CPRTEVS01.triadhospitals.net> Hi All, I need some information "Fast" Does anyone know the approx. retail price for Tissue-Tek Prisma Stainer/ Coverslipper from Sakura I just need ballpark numbers... this morning!!!! THANKS!!!! Robert L. Lott, HTL(ASCP) Manager, Anatomic Pathology LabFirst / Trinity Medical Center - formerly Montclair Baptist Medical Center 800 Montclair Road Birmingham, AL 35213 205-592-5388 phone 205-592-5646 fax robert.lott@triadhospitals.com From Margaret.Perry <@t> sdstate.edu Fri Mar 30 10:58:34 2007 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Fri Mar 30 10:58:44 2007 Subject: [Histonet] RE: background In-Reply-To: <20070329180156.09931B57FB@barracuda2.sdstate.edu> References: <20070329180156.09931B57FB@barracuda2.sdstate.edu> Message-ID: We were not able to see or listen to the NSH conference because our materials did not arrive in time. I will be working with chicken antibodies on other avian species such as turkeys. I am expecting the same problems as Mr. Sanchez-Quinteiro. I am not sure what to use as my secondary antibody. I know one of my antibodies is IgGy so I was thinking of using a biotinylated anti IgGy as my secondary. If we use the antibodies on chickens what would I do? -----Original Message----- Message: 2 Date: Wed, 28 Mar 2007 14:56:23 -0500 From: "Johnson, Teri" Subject: [Histonet] Re: background To: Message-ID: Content-Type: text/plain; charset="us-ascii" Pablo writes: >>Dear Histonetters, I am doing OMP immunohistochemistry in paraffin-embedded sections of sheep olfactory mucosa. In my positive control -mouse olfactory tissue- there is not any background, but in he sheep sections I get lot of unspecific labelling (vessels, connective tissue, respiratory mucosae). Being my primary antibody raised in goat I guess that there is an undesired cross reaction with the sheep tissue. Could I improve my blocking? I do half an hour in 2% BSA and 5% Horse normal serum. Should I use Goat normal serum? Thanks in advance for your input. Pablo Sanchez-Quinteiro Lugo (Spain)<< Luckily for you, Liz Chlipala covered this very topic in her absolutely wonderful NSH teleconference today. All you attendees out there, look at slide #48 and the answer is right there under her Antibody #2 example. One possibility for this is that your anti-goat secondary antibody shows minimal cross-reactivity with mouse, but considerable cross-reactivity with sheep. Check your antibody spec sheet and see if it's listed there. If not, call the company and ask them. Adding normal goat serum to a pre-primary antibody blocking step will most likely not have any affect if the problem is your secondary antibody. And you definitely want to stay far away from goat serum any time you are applying an anti-goat antibody to your tissues. You will get high background staining all over if you do that. Two possible fixes: Change to an anti-goat antibody that has been cross-adsorbed for sheep (or multiple species including sheep) and see if that fixes it. -or- Add 5-10% normal SHEEP serum to the diluent you use to make up your anti-goat antibody, and incubate your dilution in the tube prior to adding it to your sheep tissue. Thanks Liz! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 ------------------------------ From liz <@t> premierlab.com Fri Mar 30 11:33:43 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Mar 30 11:20:22 2007 Subject: [Histonet] RE: background In-Reply-To: Message-ID: <000001c772e9$2ef1e660$0d00a8c0@domain.Premier> Margaret What species is your primary antibody generated in and what is it detecting? I have not done work in chickens or turkeys before, but I might be able to help out. Just give me as much information as possible and I'll see what I can find out. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perry, Margaret Sent: Friday, March 30, 2007 9:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: background We were not able to see or listen to the NSH conference because our materials did not arrive in time. I will be working with chicken antibodies on other avian species such as turkeys. I am expecting the same problems as Mr. Sanchez-Quinteiro. I am not sure what to use as my secondary antibody. I know one of my antibodies is IgGy so I was thinking of using a biotinylated anti IgGy as my secondary. If we use the antibodies on chickens what would I do? -----Original Message----- Message: 2 Date: Wed, 28 Mar 2007 14:56:23 -0500 From: "Johnson, Teri" Subject: [Histonet] Re: background To: Message-ID: Content-Type: text/plain; charset="us-ascii" Pablo writes: >>Dear Histonetters, I am doing OMP immunohistochemistry in paraffin-embedded sections of sheep olfactory mucosa. In my positive control -mouse olfactory tissue- there is not any background, but in he sheep sections I get lot of unspecific labelling (vessels, connective tissue, respiratory mucosae). Being my primary antibody raised in goat I guess that there is an undesired cross reaction with the sheep tissue. Could I improve my blocking? I do half an hour in 2% BSA and 5% Horse normal serum. Should I use Goat normal serum? Thanks in advance for your input. Pablo Sanchez-Quinteiro Lugo (Spain)<< Luckily for you, Liz Chlipala covered this very topic in her absolutely wonderful NSH teleconference today. All you attendees out there, look at slide #48 and the answer is right there under her Antibody #2 example. One possibility for this is that your anti-goat secondary antibody shows minimal cross-reactivity with mouse, but considerable cross-reactivity with sheep. Check your antibody spec sheet and see if it's listed there. If not, call the company and ask them. Adding normal goat serum to a pre-primary antibody blocking step will most likely not have any affect if the problem is your secondary antibody. And you definitely want to stay far away from goat serum any time you are applying an anti-goat antibody to your tissues. You will get high background staining all over if you do that. Two possible fixes: Change to an anti-goat antibody that has been cross-adsorbed for sheep (or multiple species including sheep) and see if that fixes it. -or- Add 5-10% normal SHEEP serum to the diluent you use to make up your anti-goat antibody, and incubate your dilution in the tube prior to adding it to your sheep tissue. Thanks Liz! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1971 (20070110) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From smehta <@t> magenbiosciences.com Fri Mar 30 13:17:29 2007 From: smehta <@t> magenbiosciences.com (Shanu Mehta) Date: Fri Mar 30 13:17:34 2007 Subject: [Histonet] Sebaeous Gland Message-ID: <4C78CF3C61E6A640888226885709EFE908C243@server01.magen.local> Hello all! We are trying to embed & section sebaceous gland from skin. I was wondering if anybody had any idea on how I should go about its processing and sectioning since it is so small! Should I be using any special cassettes for processing? Any help would be greatly appreciated! Thanks, Shanu ------------ Shanu Mehta Magen Biosciences 790 Memorial Drive Suite 101 Cambridge, MA 02139 Phone: (617) 494-8732 x 2212 Fax: (617) 494-8752 Email: smehta@magenbiosciences.com http://www.magenbiosciences.com From liz <@t> premierlab.com Fri Mar 30 13:59:39 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Mar 30 13:46:18 2007 Subject: [Histonet] Sebaeous Gland In-Reply-To: <4C78CF3C61E6A640888226885709EFE908C243@server01.magen.local> Message-ID: <000201c772fd$916a65b0$0d00a8c0@domain.Premier> Shanu Are you dissecting out the sebaceous gland from the skin? How would you do that? I know for topical drug delivery studies we looked at the entire pilosebaceous unit. So we just processed the entire skin specimen and sectioned multiple levels. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shanu Mehta Sent: Friday, March 30, 2007 12:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sebaeous Gland Hello all! We are trying to embed & section sebaceous gland from skin. I was wondering if anybody had any idea on how I should go about its processing and sectioning since it is so small! Should I be using any special cassettes for processing? Any help would be greatly appreciated! Thanks, Shanu ------------ Shanu Mehta Magen Biosciences 790 Memorial Drive Suite 101 Cambridge, MA 02139 Phone: (617) 494-8732 x 2212 Fax: (617) 494-8752 Email: smehta@magenbiosciences.com http://www.magenbiosciences.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1971 (20070110) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From jamie.erickson <@t> abbott.com Fri Mar 30 13:46:55 2007 From: jamie.erickson <@t> abbott.com (Jamie E Erickson) Date: Fri Mar 30 13:47:06 2007 Subject: [Histonet] Murine perfusion for whole autoradiography In-Reply-To: <5p34dm$4q490u@cobra.abbott.com> Message-ID: HI All, I saw this posting of perfusion for mouse brain and had to ask a similar question. I am working on doing biodistrubtion of whole mice with I125 labeled antibodies. The question I have is I want to perfuse out all the radioactive blood from my mice before sectioning the whole mouse so that I can look to see where the binding might be and have little radioactivity due to blood content. If the pressure in the perfusion is to high this may push my radioactive antibody by pressure my into an area that is not where it would have gone in-vivo without perfusion giving me a false positive location. So is there a way to mimic physiological pressure in a mouse so that this would not be an issue. How high would you hang the perfusion bag for a mouse??? Any thoughts??? _______________________________ Jamie Erickson Sr. Research Associate Department: DSMP Abbott Bioresearch Center 100 Research Drive Worcester, MA 01605-4341 508-688-3134 FAX: 508-793-4895 e-mail: jamie.erickson@abbott.com From SBarnes <@t> elch.org Fri Mar 30 13:58:09 2007 From: SBarnes <@t> elch.org (Sue Barnes) Date: Fri Mar 30 13:58:34 2007 Subject: [Histonet] Paper Bag In-Reply-To: <4f016b690703300416j7d7e4c7cp7428317a70251c4e@mail.gmail.com> Message-ID: Fisher Scientific has the bags, they are called Histoprep biopsy bags 500 bags in a unit catalog no. 15-182-506H -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Victoria Baker Sent: Friday, March 30, 2007 7:17 AM To: Douglas D Deltour Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paper Bag Douglas, I have also been looking to find the paper biopsy bags, also known as "tea bags". The fellow who had gotten this product on the market passed away not that long ago. What I'm gathering from various vendors is that they are not manufacturing these bags due to some legal ranglings. At this point the only item we've been able to look at that even comes close to what we need comes from Surgipath. It's a tinted sheet that we use for our most delicate biopsies. I'm at home now so I don't have the catalog number, but if you want I would contact Surgipath directly I'm sure they can help you. It's a subsitute, not what you're looking for but it will hold up. Vikki Baker Histology Supervisor NIH/NCI/LP, Bethesda Maryland On 3/28/07, Douglas D Deltour wrote: > Hello All, > > > > I am looking for a supplier of the paper biopsy bags. Most of the sources > that I use have discontinued the paper for the nylon bags. > > > > Thanks. > > > > > > Douglas D. Deltour HT(ASCP) > > Histology Manager > > Professional Pathology Services, PC > > One Science Court > > Suite 200 > > Columbia, SC 29203 > > (803)252-1913 > > Fax (803)254-3262 > > > > ***************************************************** > > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of the individual or entity to > which it is addressed and may contain information that is privileged, > confidential and exempt from disclosure under applicable law. If the reader > of this message is not the intended recipient, you are hereby notified that > any dissemination, distribution, or copying of this communication is > strictly prohibited by law. If you have received this communication in > error, please notify me immediately. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Fri Mar 30 15:03:53 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Fri Mar 30 14:03:13 2007 Subject: [Histonet] Paper Bag In-Reply-To: Message-ID: I tried to get these but I was told that they were discontinued and replaced with the nylon bags. Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: Sue Barnes [mailto:SBarnes@elch.org] Sent: Friday, March 30, 2007 1:58 PM To: Victoria Baker; Douglas D Deltour Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Paper Bag Fisher Scientific has the bags, they are called Histoprep biopsy bags 500 bags in a unit catalog no. 15-182-506H -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Victoria Baker Sent: Friday, March 30, 2007 7:17 AM To: Douglas D Deltour Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paper Bag Douglas, I have also been looking to find the paper biopsy bags, also known as "tea bags". The fellow who had gotten this product on the market passed away not that long ago. What I'm gathering from various vendors is that they are not manufacturing these bags due to some legal ranglings. At this point the only item we've been able to look at that even comes close to what we need comes from Surgipath. It's a tinted sheet that we use for our most delicate biopsies. I'm at home now so I don't have the catalog number, but if you want I would contact Surgipath directly I'm sure they can help you. It's a subsitute, not what you're looking for but it will hold up. Vikki Baker Histology Supervisor NIH/NCI/LP, Bethesda Maryland On 3/28/07, Douglas D Deltour wrote: > Hello All, > > > > I am looking for a supplier of the paper biopsy bags. Most of the sources > that I use have discontinued the paper for the nylon bags. > > > > Thanks. > > > > > > Douglas D. Deltour HT(ASCP) > > Histology Manager > > Professional Pathology Services, PC > > One Science Court > > Suite 200 > > Columbia, SC 29203 > > (803)252-1913 > > Fax (803)254-3262 > > > > ***************************************************** > > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of the individual or entity to > which it is addressed and may contain information that is privileged, > confidential and exempt from disclosure under applicable law. If the reader > of this message is not the intended recipient, you are hereby notified that > any dissemination, distribution, or copying of this communication is > strictly prohibited by law. If you have received this communication in > error, please notify me immediately. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From stamptrain <@t> yahoo.com Fri Mar 30 14:15:02 2007 From: stamptrain <@t> yahoo.com (Roger Moretz) Date: Fri Mar 30 14:15:12 2007 Subject: [Histonet] Murine perfusion for whole autoradiography In-Reply-To: Message-ID: <649220.71035.qm@web55805.mail.re3.yahoo.com> When I said the drip bottles were at about 6ft, I forgot to emphasize that this was above the floor--the mouse was in a hood, so that's about 3ft, so the differential between the animal and the drip bottle is is the range of 3ft. You might lower the drip bottle a foot to see if that works. We did not see any extravascular spaces in the brain that would have indicated that pressure was too high, and I do not recall having seen any pressure-related artifacts in either liver, kidney or spleen (3 other organs of interest in various studies). I can't think of another way to test this other than microscopically (well, I can, but they don't seem all that attractive or particularly feasible). Roger Moretz, Ph.D. Dept of Toxicology Boehringer Ingelheim Pharmaceuticals, Inc. Ridgefield, CT --- Jamie E Erickson wrote: > HI All, > I saw this posting of perfusion for > mouse brain and had to > ask a similar question. > I am working on doing biodistrubtion of whole mice > with I125 labeled > antibodies. The question I have is I want to perfuse > out all the > radioactive blood from my mice before sectioning the > whole mouse so that > I can look to see where the binding might be and > have little radioactivity > due to blood content. If the pressure in the > perfusion is to high this > may push my radioactive antibody by pressure my > into an area that is not > where it would have gone in-vivo without perfusion > giving me a false > positive location. > So is there a way to mimic physiological > pressure in a mouse so that > this would not be an issue. How high would you hang > the perfusion bag for > a mouse??? > > Any thoughts??? > > _______________________________ > Jamie Erickson > Sr. Research Associate > Department: DSMP > Abbott Bioresearch Center > 100 Research Drive > Worcester, MA 01605-4341 > 508-688-3134 > FAX: 508-793-4895 > e-mail: jamie.erickson@abbott.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ____________________________________________________________________________________ It's here! Your new message! Get new email alerts with the free Yahoo! Toolbar. http://tools.search.yahoo.com/toolbar/features/mail/ From mcauliff <@t> umdnj.edu Fri Mar 30 14:15:11 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Mar 30 14:15:44 2007 Subject: [Histonet] Murine perfusion for whole autoradiography In-Reply-To: References: Message-ID: <460D61BF.3060600@umdnj.edu> Hi Jamie: First off is there so much I125 in the blood that it will obscure your results? Is someone going to complain that washing the blood out skews the "true physiology"? Secondly, if perfusion pressure is too high it will damage the blood vessels but in that case I think any subsequent procesing, washing, etc. would remove the blood and any bound I125? You would have to make a pretty big hole to push antibody molecules through. How high is too high? I suspect blood pressure in a mouse is not much different from any other mammal, ie humans, and such information is available. Converting inches of water (how high to hang the perfusion bottle) to millemeter of mercury is just arithmetic. 1 inch = 25.4 mm and mercury has a specific gravity of 13.6. You could also buy a peristatic pump and hook up a pressure gauge to the output side ............... Geoff Jamie E Erickson wrote: >HI All, > I saw this posting of perfusion for mouse brain and had to >ask a similar question. >I am working on doing biodistrubtion of whole mice with I125 labeled >antibodies. The question I have is I want to perfuse out all the >radioactive blood from my mice before sectioning the whole mouse so that >I can look to see where the binding might be and have little radioactivity >due to blood content. If the pressure in the perfusion is to high this >may push my radioactive antibody by pressure my into an area that is not >where it would have gone in-vivo without perfusion giving me a false >positive location. > So is there a way to mimic physiological pressure in a mouse so that >this would not be an issue. How high would you hang the perfusion bag for >a mouse??? > >Any thoughts??? > >_______________________________ >Jamie Erickson >Sr. Research Associate >Department: DSMP >Abbott Bioresearch Center >100 Research Drive >Worcester, MA 01605-4341 >508-688-3134 >FAX: 508-793-4895 >e-mail: jamie.erickson@abbott.com >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From slappycraw <@t> yahoo.com Fri Mar 30 14:38:25 2007 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Fri Mar 30 14:38:28 2007 Subject: [Histonet] Sebaeous Gland In-Reply-To: <000201c772fd$916a65b0$0d00a8c0@domain.Premier> Message-ID: <567871.41478.qm@web53612.mail.re2.yahoo.com> Just wanted to say, everyone here at Amgen in Seattle really enjoyed your tele-conference the other day. Very informative and some great tips as well, outstanding job! Liz Chlipala wrote: Shanu Are you dissecting out the sebaceous gland from the skin? How would you do that? I know for topical drug delivery studies we looked at the entire pilosebaceous unit. So we just processed the entire skin specimen and sectioned multiple levels. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shanu Mehta Sent: Friday, March 30, 2007 12:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sebaeous Gland Hello all! We are trying to embed & section sebaceous gland from skin. I was wondering if anybody had any idea on how I should go about its processing and sectioning since it is so small! Should I be using any special cassettes for processing? Any help would be greatly appreciated! Thanks, Shanu ------------ Shanu Mehta Magen Biosciences 790 Memorial Drive Suite 101 Cambridge, MA 02139 Phone: (617) 494-8732 x 2212 Fax: (617) 494-8752 Email: smehta@magenbiosciences.com http://www.magenbiosciences.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1971 (20070110) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Don't pick lemons. See all the new 2007 cars at Yahoo! Autos. From tilman.fuldatal <@t> web.de Fri Mar 30 14:40:47 2007 From: tilman.fuldatal <@t> web.de (Tilman Krieger) Date: Fri Mar 30 15:00:54 2007 Subject: [Histonet] Problems using Pap Pen In-Reply-To: References: Message-ID: <460D67BF.9050305@web.de> Hello and thanks a lot for all your advices. I could perform a much better staining of my brain sections now. I used the same protocol as before but this time I made draw the circles with my PAP-Pen as big as possible - about 5 millimeters away from the tissue. and this time I coud avoud staining just fragmants of the brain. Of course I had to use a bit more fluid for every section (40 mycroliters each). But it was worth it. I also wondered if it is necessary to let the PAP-Pen dry or not. I tested this by letting some of my sections dry for 10, others for about 30 seconds, at so I blew, others were directly given into BSA-solution. My result: There is NO difference at all. The idea of using normal wax pencils instead of a PAP-Pen sounds interesting - would be much cheaper. But I did not test this yet. Thanks agagin. Sincerely, Til / MR From katherine-walters <@t> uiowa.edu Fri Mar 30 15:02:25 2007 From: katherine-walters <@t> uiowa.edu (Walters, Katherine S) Date: Fri Mar 30 15:02:32 2007 Subject: [Histonet] DAB & Masson's compatability Message-ID: Question-Will a slide stained with DAB hold up to a Masson's trichrome stain? I am worried about losing the DAB precipitate. Specifically, I want a stain that will demonstrate fibrosis and a cytokine antibody on the same slide. Does anyone have any ideas about how to do this? TIA, Kathy From liz <@t> premierlab.com Fri Mar 30 15:32:49 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Mar 30 15:19:23 2007 Subject: [Histonet] DAB & Masson's compatability In-Reply-To: Message-ID: <000001c7730a$95661170$0d00a8c0@domain.Premier> Kathy I have not personally tried a trichrome, but I have done Acid Fast, Cresyl echt violet, iron, etc on DAB stained slides. We have ran both the IHC first and on other instances we have ran the special first (iron with CD68 IHC with Fast Red Chromagen). Just run your immuno as usual and then rinse in distilled water and follow with the trichrome, the only thing that I'm not sure of is if the DAB will survive the bouins, but I think it might, since I can't think of anything that will remove DAB from a slide. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Walters, Katherine S Sent: Friday, March 30, 2007 2:02 PM To: histonet@pathology.swmed.edu Subject: [Histonet] DAB & Masson's compatability Question-Will a slide stained with DAB hold up to a Masson's trichrome stain? I am worried about losing the DAB precipitate. Specifically, I want a stain that will demonstrate fibrosis and a cytokine antibody on the same slide. Does anyone have any ideas about how to do this? TIA, Kathy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1971 (20070110) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From mcauliff <@t> umdnj.edu Fri Mar 30 15:26:05 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Mar 30 15:25:36 2007 Subject: [Histonet] DAB & Masson's compatability In-Reply-To: References: Message-ID: <460D725D.4020506@umdnj.edu> I would think it would be fine as long as you avoid strong acids. Try it on a spare slide before doing the important stuff. Also, intensifying the DAB with nickel, nickel+cobalt, osmium vapors, silver or a commercial product like Vector's Intense would be worth a try. Geoff Walters, Katherine S wrote: >Question-Will a slide stained with DAB hold up to a Masson's trichrome >stain? I am worried about losing the DAB precipitate. Specifically, I >want a stain that will demonstrate fibrosis and a cytokine antibody on >the same slide. Does anyone have any ideas about how to do this? >TIA, >Kathy > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From ftulenko06 <@t> jcu.edu Fri Mar 30 16:35:46 2007 From: ftulenko06 <@t> jcu.edu (ftulenko06@jcu.edu) Date: Fri Mar 30 16:35:55 2007 Subject: [Histonet] HRP Message-ID: <20070330173546.ABQ65914@mirapoint.jcu.edu> Does anyone know of any commercially available substrates for peroxidase besides DAB or 3-amino 9-ethyl carbazole that yield colored precipitates? Any info would be appreciated. Thanks, Frank From escott8 <@t> houston.rr.com Fri Mar 30 18:54:50 2007 From: escott8 <@t> houston.rr.com (Edward Scott) Date: Fri Mar 30 18:54:53 2007 Subject: [Histonet] CAP Inspection Message-ID: <004701c77326$cf3e0c10$6500a8c0@thescotts> Our lab was just inspected today (unannounced) by CAP. We had no deficiencies or recomendations. It was a tough inspection team from quest Labs. Have your employees training records at hand and make sure all of your reagents have open and received dates on them. What a way to end the week . Margaritas for everyone. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston Texas From dlcowie <@t> prodigy.net Sat Mar 31 05:26:47 2007 From: dlcowie <@t> prodigy.net (Dawn Cowie) Date: Sat Mar 31 05:26:54 2007 Subject: [Histonet] delivery time of last tray of H&E In-Reply-To: <270614B321ACB44D8C1D91F4F921FDC304B163D5@NCH01EX02.nch.org> Message-ID: <534443.11193.qm@web81001.mail.mud.yahoo.com> Hello Laura, Thanks for the info on the RHS. What do you mean by "limitless system"? Where is your lab located? I would like to see another lab running this microwave. Thanks, Dawn "Galiotto, Laura" wrote: Hello Dawn, We have the RHS-1 and are getting a second one. I like the limitless system. We are processing tissue as thick as 3mm starting the run at 10:30 and handing in the slides at 16:00. It also does not restrict you to a different reagent or vendor for reagents. I continue to use 10%NBF for fixation and ethanol & Isopropanol for dehydration, then immediately into paraffin. If you are ever in town stop by to see it. Thanks Laura -----Original Message----- From: Dawn Cowie [mailto:dlcowie@prodigy.net] Sent: Friday, March 23, 2007 10:11 AM To: Galiotto, Laura Subject: RE: [Histonet] delivery time of last tray of H&E Laura, It is definitely hard to find certified techs willing to do those hours. I'm lucky at the moment - the techs that work these hours want to. I'm not sure what we'll do when 1 of them retires probably in the next couple of years. What kind of microwave processor do you run? We are going to look at this again. We tried the RHS-1 a few years ago- docs didn't like results - too labour intensive for the techs. thanks for your response, Dawn "Galiotto, Laura" wrote: Hello Dawn, Yeah, however you do bring up a very valid point. I do get the specialist, ie.. oncologist,pulmonaryologist, that come in very early to check slides before the pathologist gets in. My biggest problem is finding certified technicians that will work the night shift, practically non-existant. Thank goodness for our microwave processor. We now are processing most biopsies sameday. Laura Galiotto, HT(ASCP) Histology Manager --------------------------------- From: Dawn Cowie [mailto:dlcowie@prodigy.net] Sent: Fri 3/23/2007 3:03 AM To: Douglas D Deltour; 'Marshall Terry Dr, Consultant Histopathologist'; 'Akemi Allison-Tacha'; 'Rene J Buesa'; Galiotto, Laura; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] delivery time of last tray of H&E We do what we are required to do by the pathologists. I don't know why they think they need all H&E's so early - they can't look at them all at the same time and sign them out - except that they have them just in case a clinician calls. The trend these days is certainly toward faster and faster turnaround time. Dawn L. Cowie, HT (ASCP) Histology Supervisor Pensacola Pathologists, PA Pensacola, Florida 32503 850-416-7251 Douglas D Deltour wrote: I don't get it either. We have a little histo pixies that comes in while we sleep and the slides magically appear on the pathologist desk in the morning. :) Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Thursday, March 22, 2007 12:28 PM To: Akemi Allison-Tacha; Rene J Buesa; Galiotto, Laura; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] delivery time of last tray of H&E This is all bloody unbelievable. Don't any of you sleep? What is the point of working at night? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: 22 March 2007 17:25 To: Rene J Buesa; Galiotto, Laura; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] delivery time of last tray of H&E Ren?--That was similar to the protocol I followed in the old days before 24/6 . We provided rush's 1st, followed by bx's by 8AM, we did however, provide special stains and recuts and IHC's by 2-3 PM, depending if it wasn't a lengthy procedure. If you have off site facilities to provide for, that is a different situation. You have to work around courier sheduals for delivering to off site facilities. Everyone needs their slides and time is money!! With the new microwave processing technology that is now in use, there has been a shift to having the majority of staff starting at 10 PM at night. MUCH TO THE DISLIKING OF HISTOTECH"S. The day shift is minimum. IHC is done during the day shift, after the pathologist has determined to do so. In most situations, a battery of IHC's tests are pre-set, as well as with special stains. Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting & Staffing Specializing in Histology, SS, IHC, & Microarray Madison, WI Tele: (925) 788-0900 E-Mail: akemiat3377@yahoo.com --- Rene J Buesa wrote: > Laura: > For a laboratory I used to manage with quite similar annual workload > we had the following > schedule: > 1- last tissue accepted at 7:00 PM; > 2- two VIPs atarting with delay to be finished at 4:00 AM next day; > 3- Rushes ready for the pathologists at 8:00 AM next day; > 4-All slides ready, the latest, at 1:00 PM. > 5- All IHC ready by 2 PM > 6- all HC ready by noon. > Ren? J. > > "Galiotto, Laura" wrote: > Hello Fellow Histo Tech's, > Will anyone be willing to share the delivery time of the last tray of > H&E for the days workload to the pathologist reading them? > What is your cutoff time for specimen acceptance? > What is your annual case workload? > I am trying to improve the TAT from specimen gross to the H&E > delivered to the pathologist. Unless we change the cutoff time I can > not see how I can do this? Any suggestions? > We process 22,000 cases a year an average of 280 blks a day consisting > of types of tissue. > Please Help > Laura > > > ******************* PLEASE NOTE ******************* This > E-Mail/telefax message and any documents accompanying this > transmission may contain information that is privileged, confidential, > and/or exempt from disclosure under applicable law and is intended > solely for the addressee(s) named above. If you are not the intended > addressee/recipient, you are hereby notified that any use of, > disclosure, copying, distribution, or reliance on the contents of this > E-Mail/telefax information is strictly prohibited and may result in > legal action against you. Please reply to the sender advising of the > error in transmission and immediately delete/destroy the message and > any accompanying documents. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Sucker-punch spam with award-winning protection. > Try the free Yahoo! Mail Beta. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************* PLEASE NOTE ******************* This E-Mail/telefax message and any documents accompanying this transmission may contain information that is privileged, confidential, and/or exempt from disclosure under applicable law and is intended solely for the addressee(s) named above. If you are not the intended addressee/recipient, you are hereby notified that any use of, disclosure, copying, distribution, or reliance on the contents of this E-Mail/telefax information is strictly prohibited and may result in legal action against you. Please reply to the sender advising of the error in transmission and immediately delete/destroy the message and any accompanying documents. Thank you. ******************* PLEASE NOTE ******************* This E-Mail/telefax message and any documents accompanying this transmission may contain information that is privileged, confidential, and/or exempt from disclosure under applicable law and is intended solely for the addressee(s) named above. If you are not the intended addressee/recipient, you are hereby notified that any use of, disclosure, copying, distribution, or reliance on the contents of this E-Mail/telefax information is strictly prohibited and may result in legal action against you. Please reply to the sender advising of the error in transmission and immediately delete/destroy the message and any accompanying documents. Thank you. From gu.lang <@t> gmx.at Sat Mar 31 05:52:45 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Mar 31 05:52:51 2007 Subject: AW: [Histonet] DAB & Masson's compatability In-Reply-To: <460D725D.4020506@umdnj.edu> Message-ID: <000001c77382$b7478990$6412a8c0@dielangs.at> The trichrome-dyesolutions usually have a ph from 1-3. I think, that is strongly acid. My tipp is, to try it on a control slide and see what happens. Or just rinse the ready stained ihc-slide in an acid solution. If you don't mind, that the trichrome results aren't very brilliant, you can ommit the bouin-step. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Geoff McAuliffe Gesendet: Freitag, 30. M?rz 2007 22:26 An: Walters, Katherine S Cc: histonet@pathology.swmed.edu Betreff: Re: [Histonet] DAB & Masson's compatability I would think it would be fine as long as you avoid strong acids. Try it on a spare slide before doing the important stuff. Also, intensifying the DAB with nickel, nickel+cobalt, osmium vapors, silver or a commercial product like Vector's Intense would be worth a try. Geoff Walters, Katherine S wrote: >Question-Will a slide stained with DAB hold up to a Masson's trichrome >stain? I am worried about losing the DAB precipitate. Specifically, I >want a stain that will demonstrate fibrosis and a cytokine antibody on >the same slide. Does anyone have any ideas about how to do this? >TIA, >Kathy > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> aol.com Sat Mar 31 16:37:03 2007 From: rsrichmond <@t> aol.com (rsrichmond@aol.com) Date: Sat Mar 31 16:37:09 2007 Subject: [Histonet] Re: Paper Bags In-Reply-To: <200703311401.288460ea1ec34d@rly-yg04.mx.aol.com> References: <200703311401.288460ea1ec34d@rly-yg04.mx.aol.com> Message-ID: <8C941F3DB9932BF-17E4-4A98@webmail-da05.sysops.aol.com> Why would anyone want to use paper rather than the present-day nylon specimen bags? I recently looked at the Fisher Web site and was astonished to learn from the fisherhealthcare.com Web site, accessed a few days ago: biopsy bag 30 x 50 mm, Fisherbrand 15-182-116 500 bags for $228.17 in contrast, Fisher offers biopsy foam pads, 22-038221 1000 rectangular pads for $73.91 In other words, those nylon bags list at around 45 cents US each, while the little blue foam pads are around 7 cents each, or half that if you cut them in two as I usually do. Finding this out certainly made me change the way I use these two items - put small discrete biopsy specimens on blue pads (marked with a small drop of safranin solution), and reserve the bags for small curettage specimens, cell blocks, and things like that. I've used these two items in a good man pathology practices, but never knew the cost of them before, since catalogs are always locked up in the lab manager's office and not available to histotechnologists. Will some of you Good Managers enlighten me as to why it's Good Management Practice to have bench techs not know the cost of the items they work with? Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From rsrichmond <@t> aol.com Sat Mar 31 17:07:51 2007 From: rsrichmond <@t> aol.com (rsrichmond@aol.com) Date: Sat Mar 31 17:08:02 2007 Subject: [Histonet] Re: Posting Images In-Reply-To: <200703301401.8bd460d50613a6@rly-ma07.mail.aol.com> References: <200703301401.8bd460d50613a6@rly-ma07.mail.aol.com> Message-ID: <8C941F828893639-17E4-4B1B@webmail-da05.sysops.aol.com> Sonya asks: >>Does anyone know of a site out there where we can post microscopy images?<< Ed Uthman, a surgical pathologist who posts a lot of photomicrographs to the Web, strongly recommends the flickr Web site for this purpose. Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From arvidsonkristen <@t> yahoo.com Sat Mar 31 17:21:20 2007 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Sat Mar 31 17:21:24 2007 Subject: [Histonet] Peloris processor Message-ID: <655396.94922.qm@web61311.mail.yahoo.com> Does anyone use the Peloris double chamber rapid tissue processor? What's it like? --------------------------------- Get your own web address. Have a HUGE year through Yahoo! Small Business. From Eric <@t> ategra.com Thu Mar 29 18:27:17 2007 From: Eric <@t> ategra.com (Eric Dye (ext 223)) Date: Sat Mar 31 19:07:57 2007 Subject: [Histonet] Histology jobs thruout the U.S. updated as of 3/29/07 Message-ID: Hi - Histonetters - How are things at ? I have both permanent and full time (permanent) J0BS for HistoTechs throughout the US. These permanent and temporary HISTOTECH J0BS are being filled quickly, don't miss out on your dream position - callmetoday ----------------------------------- Temporary HISTOTECH J0BS : ----------------------------------- (Updated Mar 29 2007) 1. South Carolina - Bench - 3 months (starting in August) -Interviewing immediately 2. Florida (East coast) bench- 6 weeks - Need Florida Licence 3. Rhode Island- 3 months - R.I license eligible- mohs- HOT 4. Eastern Massachusetts - 3 months - Routine Histology- MUST be ASCP certified 5. New Hampshire - 3 months - Routine Histology 6. California (Hollywood) -3-6 months 7. Ohio (Cleveland area) - 1 month - Routine Histology 8. California - (San diego area) - 2 months -Routine Histology ------------------------------------------------------- New Permanent HISTOTECHJ0BS Listed below ------------------------------------------------------- 1. Central Florida -Histotech-Bench-perm (BrandNew, HOT) 2. Michigan (Ann Arbor Area) Histotech- Supervisor- perm 3. Northern Ohio- Histotech - bench - perm 4. Eastern Pennsylvania - Histotech - Bench - perm 5. Florida( Tampa Bay area) Histotech- bench- perm 6. Massachusetts (Greater Boston Area) Histotech- Bench - perm 7. Massachusetts ( Greater Boston Area) Histotech Supervisor 8. Western Pennsylvania - Bench - perm 9. - filled - 10. Kentucky (Northwestern)- Bench - perm - 2 openings!! 11. Virginia (Close to North Carolina Border!!) bench- perm 12. Wisconsin - Supervisor - perm 13. Filled 14. Ohio (Northern) perm- Bench 15. Ohio (Central) perm- Bench 16 .Central Florida -perm- Histotech (Need Florida License) 17. Florida (Tampa Bay area) need Florida license 18. Las Vegas - Bench Histotech- perm 19. Virginia - Histotech- perm- Bench- 20mins from North Carolina Border 20. Virginia (Central)- Histotech- perm -bench- BrandNew -------------------------- end list of Histotech Opportunities ------------------------------------- If you are interested in any of the HISTOTECH J0BS listed above - please call me at 800-466-9919 ext 223 or cell - 407-756-5507. Thank you, Eric Dye-Sr Allied Healthcare Recruiter 800-466-9919 ext 223 or Cell - 407-756-5507 P.S.: Feel free to pass along this email and my phone number to anyone who you think might be interested. P.S.S.: The clients are currently interviewing - and the positions will close soon - so if you are interested, please call me today at 1-800-466-9919 ext 229 --------------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing 800-466-9919 ext 223 - voice 407 671 6075 - fax eric@ategra.com To Learn More About Ategra: http://www.ategra.com ---------------------------------------------------------------- If you received this by mistake, or if you wish not to hear from me, please shoot me a mail to let me know and I'll not mail you again. ---------------------------------------------------------------- ---------------------------------------------------------------- Note: this message is intended for: Histonetters at histonet@lists.utsouthwestern.edu ----------------------------------------------------------------