[Histonet] Re: Histonet Digest, Vol 43, Issue 40

[Asf2k3 <@t> aol.com]

What is the best way to avoid having bubbles in mold when embedding  tissue. 
We just use regular melted parrafin in embedding center. What I did is  put 
parraffin in mold, then put in on cold plate to start freezing a bit, then  put 
the tissue so it will adhere to mold, then put the cassette top on mold, and  
fill it up with parrafin. I do this step but still see bubbles forming. I tap  
the cassette top on mold to get rid of bubbles which works, but this step is 
not  convenience if you have to embed quickly because I have to embed like 120 
blocks  in hr and half.
 
A
 
Please let me know. 



************************************** See what's free at http://www.aol.com.