[Histonet] Re: Histonet Digest, Vol 43, Issue 35

[Emily Sours]

I just realized something that others may not do--our paraffin is
melted in a vacuum oven, which decreases the amount of bubbles in the
paraffin.  Do you use one of these? This may be more valuable than I
realize, since I've never used paraffin without a vacuum oven.
To answer your other email, we leave our blocks at 4C anywhere from
overnight to a month later.  Again, I've never noticed a difference
when sectioning cold paraffin blocks from room temperature blocks.

Emily
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