[Histonet] CD4 and CD8 Staining

[CLAIRE ANN KENTLER]

Hi all,

I'm relatively new to all this, but I am looking for an
immunohistochemical stain for CD4 and CD8 in calf tissue.  I was hoping
to find a protocol for formalin-fixed paraffin embedded sections, as
bovine WC1 can be isolated in these sections and I am looking at cell
proliferation and T and B lymphocyte percentages in this manner.  All
the literature I've come across indicates the need for either a modified
fixative, or frozen sections.  (Thus requiring double the sample
collection, or changing of previous protocols and study repetition).

Has anyone come across a good retrieval method for CD4 and CD8 in ffpe
sections that we could try in bovine tissue, or are frozen sections the
way to go?

Thanks,
Claire

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Claire Kentler
Bachelor of Animal Science (Hons)
Postgraduate Student
Department of Agricultural Sciences
La Trobe University
Phone: 9479 1048
E-mail: cabramley <@t> students.latrobe.edu.au