[Histonet] Poor eosin staining following prolonged fixation - summary

[Keeping, Janet]

Thanks you to all the people who responded. A summary of the suggestions
I received follow:

 

*	Following bluing-use 1 minute water wash followed by 70% alcohol
then place in Eosin
*	No 70% alcohol after Eosin, 95%  is dilute enough
*	Use fresher tissues
*	Add phloxine to the Eosin
*	Treat slides with permanganate and oxalic acid before staining
*	Check whether Eosin is water or alcohol soluble 

 

Janet Keeping A.R.T., B. Voc. Ed

Instructor

Medical Laboratory Sciences

 

1 Prince Philip drive

P.O. Box 1693, St. John's 

NL, Canada A1C 5P7

709 758 7657 tel

709 758 7635 fax

E-mail; janet.keeping <@t> cna.nl.ca