From talulahgosh <@t> gmail.com Fri Jun 1 00:07:38 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Jun 1 00:07:42 2007 Subject: [Histonet] certification Message-ID: What exactly makes one a certified HT or HTL tech? Does this vary by state? I've never heard of it and googling it isn't helping. (my apologies to Greg Luck for the multiple emails!) Emily -- Pray, v.: To ask that the laws of the universe be annulled on behalf of a single petitioner confessedly unworthy. -Ambrose Bierce From JMacDonald <@t> mtsac.edu Fri Jun 1 01:08:29 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Jun 1 01:08:35 2007 Subject: [Histonet] certification In-Reply-To: Message-ID: Emily, The ASCP (American Society for Clinical Pathology) certifies laboratory personnel. This is a national certification, recognized by all states, although there are some states that require a license in addition. In order to become certified you are required to take an exam. To be eligible for the HT certification examination, you must have an AS degree and one year of experience in a histology laboratory or graduate from a NAACLS accredited program. For the HTL level certification a BS degree is required and one year experience, or the BS degree and graduation from a NAACLS accredited program. Visit www.ascp.org and go to the Board of Registry (BOR) area Should you have any other questions please feel free to contact me. Jennifer Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu "Emily Sours" Sent by: histonet-bounces@lists.utsouthwestern.edu 05/31/2007 10:07 PM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] certification What exactly makes one a certified HT or HTL tech? Does this vary by state? I've never heard of it and googling it isn't helping. (my apologies to Greg Luck for the multiple emails!) Emily -- Pray, v.: To ask that the laws of the universe be annulled on behalf of a single petitioner confessedly unworthy. -Ambrose Bierce _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> CDC.GOV Fri Jun 1 04:44:57 2007 From: jqb7 <@t> CDC.GOV (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Fri Jun 1 04:45:19 2007 Subject: [Histonet] certification In-Reply-To: References: Message-ID: <34BB307EFC9A65429BBB49E330675F7201B0B78E@LTA3VS003.ees.hhs.gov> All, While Jennifer is absolutely correct I must mention one issue. I know of hospital labs that give their techs the title of HT or HTL based on education (degree vs. non-degree) BUT requires no ASCP examination. Basically, some hospitals simply bestow the title of HTL is you have a Bachelor's and get trained on the job. If you do not have the degree but they train you they give you the title of HT. So unless you see ASCP after that HT or HTL, it may not mean what you think it does. Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Friday, June 01, 2007 2:08 AM To: Emily Sours Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] certification Emily, The ASCP (American Society for Clinical Pathology) certifies laboratory personnel. This is a national certification, recognized by all states, although there are some states that require a license in addition. In order to become certified you are required to take an exam. To be eligible for the HT certification examination, you must have an AS degree and one year of experience in a histology laboratory or graduate from a NAACLS accredited program. For the HTL level certification a BS degree is required and one year experience, or the BS degree and graduation from a NAACLS accredited program. Visit www.ascp.org and go to the Board of Registry (BOR) area Should you have any other questions please feel free to contact me. Jennifer Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu "Emily Sours" Sent by: histonet-bounces@lists.utsouthwestern.edu 05/31/2007 10:07 PM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] certification What exactly makes one a certified HT or HTL tech? Does this vary by state? I've never heard of it and googling it isn't helping. (my apologies to Greg Luck for the multiple emails!) Emily -- Pray, v.: To ask that the laws of the universe be annulled on behalf of a single petitioner confessedly unworthy. -Ambrose Bierce _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Fri Jun 1 07:29:21 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Jun 1 07:29:34 2007 Subject: [Histonet] certification In-Reply-To: <34BB307EFC9A65429BBB49E330675F7201B0B78E@LTA3VS003.ees.hhs.gov> Message-ID: <000001c7a448$7e43c680$d00f7ca5@lurie.northwestern.edu> All, Just remember as of January 2005 the on-the-job training for HT was removed from BOR certification pathway. I don't know of anyone bestowed a title just by education (fortunately) I do have issues with uncertified people doing histo with no knowledge of what they are actually doing and belittling the fact that I made an effort to get my HTL. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Friday, June 01, 2007 4:45 AM To: Jennifer MacDonald; Emily Sours Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] certification All, While Jennifer is absolutely correct I must mention one issue. I know of hospital labs that give their techs the title of HT or HTL based on education (degree vs. non-degree) BUT requires no ASCP examination. Basically, some hospitals simply bestow the title of HTL is you have a Bachelor's and get trained on the job. If you do not have the degree but they train you they give you the title of HT. So unless you see ASCP after that HT or HTL, it may not mean what you think it does. Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Friday, June 01, 2007 2:08 AM To: Emily Sours Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] certification Emily, The ASCP (American Society for Clinical Pathology) certifies laboratory personnel. This is a national certification, recognized by all states, although there are some states that require a license in addition. In order to become certified you are required to take an exam. To be eligible for the HT certification examination, you must have an AS degree and one year of experience in a histology laboratory or graduate from a NAACLS accredited program. For the HTL level certification a BS degree is required and one year experience, or the BS degree and graduation from a NAACLS accredited program. Visit www.ascp.org and go to the Board of Registry (BOR) area Should you have any other questions please feel free to contact me. Jennifer Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu "Emily Sours" Sent by: histonet-bounces@lists.utsouthwestern.edu 05/31/2007 10:07 PM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] certification What exactly makes one a certified HT or HTL tech? Does this vary by state? I've never heard of it and googling it isn't helping. (my apologies to Greg Luck for the multiple emails!) Emily -- Pray, v.: To ask that the laws of the universe be annulled on behalf of a single petitioner confessedly unworthy. -Ambrose Bierce _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Jun 1 07:31:00 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jun 1 07:31:04 2007 Subject: [Histonet] processing fatty tissue In-Reply-To: <387005.30260.qm@web58913.mail.re1.yahoo.com> Message-ID: <768364.8999.qm@web61221.mail.yahoo.com> Robert: Under separate cover I am sending you details of my procedure. I am Sure Maxim will do the same. Ren? J. Robert Chiovetti wrote: Maxim, Rene (and Other Histonetters), That's interesting re: using either a mix of ethanol+isopropanol+mineral oil (Rene) or isopropanol+mineral oil (Maxim) for breast tissue. Could you share your recipes with us? I have a customer (derm path) who could probably benefit from this for larger and thicker skin specimens which sometimes have a lot of subcutaneous fat associated with them. Thanks in advance, if you could share the recipes! Cheers, Bob Robert (Bob) Chiovetti, Ph.D. Southwest Precision Instruments The Desert Southwest's Microscopy Resource Tucson, Arizona USA Tel./Fax 520-546-4986 www.swpinet.com Member, Arizona Small Business Association (www.asba.com) ----- Original Message ---- From: Maxim Peshkov To: ROrr@enh.org Cc: histonet@lists.utsouthwestern.edu; Gervaip@aol.com Sent: Thursday, May 31, 2007 12:05:21 PM Subject: Re: [Histonet] processing fatty tissue I completely agree with Rene. When we processed breasts (and other tissues) with isopropanol+mineral oil that we ceased to feel the difficulties with our samples. We have manually processing. More thanks to Dr. McCormick for cassettes! Sincerely, Maxim Peshkov Russia Taganrog "Rene J Buesa" wrote: Date: Tue, 29 May 2007 06:22:59 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] processing fatty tissue To: "Orr, Rebecca" , histonet@lists.utsouthwestern.edu Cc: Gervaip@aol.com 1- thin slices 2- well fixed after the slices are prepared (at least 8 h) 3- use fresh alcohols for dehydration 4- extend somewaht (20%) the clearing steps time (time x 1.2), and 5- if possible use a 60?C paraffin, OR process with ethanol followed by a mixture of ethanol+propanol+mineral oil, and breats will cut "like butter". Ren? J. ____________________________________________________________________________________ Be a PS3 game guru. Get your game face on with the latest PS3 news and previews at Yahoo! Games. http://videogames.yahoo.com/platform?platform=120121 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better Heartthrob. Get better relationship answers from someone who knows. Yahoo! Answers - Check it out. From jqb7 <@t> CDC.GOV Fri Jun 1 07:31:38 2007 From: jqb7 <@t> CDC.GOV (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Fri Jun 1 07:31:55 2007 Subject: [Histonet] certification In-Reply-To: <000001c7a448$7e43c680$d00f7ca5@lurie.northwestern.edu> References: <34BB307EFC9A65429BBB49E330675F7201B0B78E@LTA3VS003.ees.hhs.gov> <000001c7a448$7e43c680$d00f7ca5@lurie.northwestern.edu> Message-ID: <34BB307EFC9A65429BBB49E330675F7201B0B792@LTA3VS003.ees.hhs.gov> Amen! Jeanine Bartlett Infectious Disease Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: Bernice Frederick [mailto:b-frederick@northwestern.edu] Sent: Friday, June 01, 2007 8:29 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); 'Jennifer MacDonald'; 'Emily Sours' Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] certification All, Just remember as of January 2005 the on-the-job training for HT was removed from BOR certification pathway. I don't know of anyone bestowed a title just by education (fortunately) I do have issues with uncertified people doing histo with no knowledge of what they are actually doing and belittling the fact that I made an effort to get my HTL. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Friday, June 01, 2007 4:45 AM To: Jennifer MacDonald; Emily Sours Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] certification All, While Jennifer is absolutely correct I must mention one issue. I know of hospital labs that give their techs the title of HT or HTL based on education (degree vs. non-degree) BUT requires no ASCP examination. Basically, some hospitals simply bestow the title of HTL is you have a Bachelor's and get trained on the job. If you do not have the degree but they train you they give you the title of HT. So unless you see ASCP after that HT or HTL, it may not mean what you think it does. Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Friday, June 01, 2007 2:08 AM To: Emily Sours Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] certification Emily, The ASCP (American Society for Clinical Pathology) certifies laboratory personnel. This is a national certification, recognized by all states, although there are some states that require a license in addition. In order to become certified you are required to take an exam. To be eligible for the HT certification examination, you must have an AS degree and one year of experience in a histology laboratory or graduate from a NAACLS accredited program. For the HTL level certification a BS degree is required and one year experience, or the BS degree and graduation from a NAACLS accredited program. Visit www.ascp.org and go to the Board of Registry (BOR) area Should you have any other questions please feel free to contact me. Jennifer Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu "Emily Sours" Sent by: histonet-bounces@lists.utsouthwestern.edu 05/31/2007 10:07 PM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] certification What exactly makes one a certified HT or HTL tech? Does this vary by state? I've never heard of it and googling it isn't helping. (my apologies to Greg Luck for the multiple emails!) Emily -- Pray, v.: To ask that the laws of the universe be annulled on behalf of a single petitioner confessedly unworthy. -Ambrose Bierce _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Jun 1 07:52:38 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jun 1 07:52:46 2007 Subject: Fwd: Re: [Histonet] processing fatty tissue Message-ID: <626934.83157.qm@web61213.mail.yahoo.com> Maxim: My answer to Robert without the attachment. Ren? J. Rene J Buesa wrote: Date: Fri, 1 Jun 2007 05:48:45 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] processing fatty tissue To: Robert Chiovetti Robert: The procedure is attached. The fundament is a GENTLE dehydrant substitution by the infiltrating agent (paraffin) and ELIMINATES the use of an antemedium (xylene). The general function of an antemedium is the ability to be the "connection" between the dehydrant and the wax (paraffin) because it mixes with both. Xylene (as well as white naphtha or some other aromatic chemicals) can do this, BUT the thing is that mineral oil (MO) IS paraffin of a low molecular weight so the antemedium is not needed or a mixture of MO with alcohols constitutes the antemedium. My procedure uses EthOL to dehydrate and later the antemedium is substituted by the mixture of EthOL + Isopropanol + MO Maxim has simplified the procedure because he process manually and don't have the advantage of vacuum or pressure or agitation as I did when developed the method. So it turns out that Maxim's modification is easier and more direct; he just dehydrates with propanol and later goes into the gentle substitution with a mixture of 5 parts of propanol + 1 part of MO heated at 50?C followed by another mixture of 2 parts of propanol + 1 of MO heated also at ?C to obtain the gentle and complete infiltration of ANY type of tissue. The infiltration with MO gives the tissues a softness never achieved with any other antemedium. You will see when you try it. Maxim's method is simpler than mine and, in the long run, will be more acceptable to all histotechs and also meets the objective of eliminating xylene from the histology lab. The procedure uses 2 chemicals that are cheaper and when needing to be disposed off, the propanol can be evaporated, the used MO mixed with used paraffin and both disposed off as a solid, cutting costs also in disposal. Try it, you will like it! Ren? J. Robert Chiovetti wrote: Maxim, Rene (and Other Histonetters), That's interesting re: using either a mix of ethanol+isopropanol+mineral oil (Rene) or isopropanol+mineral oil (Maxim) for breast tissue. Could you share your recipes with us? I have a customer (derm path) who could probably benefit from this for larger and thicker skin specimens which sometimes have a lot of subcutaneous fat associated with them. Thanks in advance, if you could share the recipes! Cheers, Bob Robert (Bob) Chiovetti, Ph.D. Southwest Precision Instruments The Desert Southwest's Microscopy Resource Tucson, Arizona USA Tel./Fax 520-546-4986 www.swpinet.com Member, Arizona Small Business Association (www.asba.com) ____________________________________________________________________________________ Be a PS3 game guru. Get your game face on with the latest PS3 news and previews at Yahoo! Games. http://videogames.yahoo.com/platform?platform=120121 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! oneSearch: Finally, mobile search that gives answers, not web links. --------------------------------- Building a website is a piece of cake. Yahoo! Small Business gives you all the tools to get online. From mirkka.sarparanta <@t> helsinki.fi Fri Jun 1 08:08:26 2007 From: mirkka.sarparanta <@t> helsinki.fi (Mirkka Sarparanta) Date: Fri Jun 1 08:08:42 2007 Subject: [Histonet] ImmEdge pen and Menzel SuperFrost Plus slides Message-ID: <46601A4A.1010000@helsinki.fi> Hi everyone! Does anyone have any experience with using the ImmEdge or DAKO pens on Menzel SuperFrost Plus adhesion slides? Vector doesn't really specify on which glass slides the pen can be used, but some such as the Super PAP pen and DAKO pen should be used only on albumin or polylysine coated slides (according to the manufacturer, anyhow). I know of one group that uses the DAKO pen succesfully on Superfrost Plus slides, but I'd like to have some second opinions before making the purchase. Your help and expertise will be greatly appreciated. Cheers, Mirkka -- Mirkka Sarparanta M.Sc., Ph.D. Student University of Helsinki Laboratory of Radiochemistry Tracers in Molecular Imaging Team e-mail: mirkka.sarparanta@helsinki.fi From rcharles <@t> state.pa.us Fri Jun 1 09:23:43 2007 From: rcharles <@t> state.pa.us (Charles, Roger) Date: Fri Jun 1 09:23:50 2007 Subject: [Histonet] RE: -20C acetone fixative Message-ID: <12E4E17FEF6EBE4BAE95BEB3CDCB57000943BA72@enhbgpri04.backup> Thanks to all that responded to this question. It's interesting to see a variety of reasons why acetone is used at this temp. I was once told by a researcher that "it's possibly due to how acetone was stored many moons ago and the practice just carried thru since." Roger Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 -----Original Message----- From: Charles, Roger Sent: Tuesday, May 22, 2007 2:29 PM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: -20C acetone fixative Does any know or remember why acetone, when used as a fixative, is always used "ice cold" or in our case at -20C? Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 From rjbuesa <@t> yahoo.com Fri Jun 1 09:27:00 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jun 1 09:27:05 2007 Subject: Fwd: RE: Re: [Histonet] processing fatty tissue Message-ID: <41938.33094.qm@web61211.mail.yahoo.com> For "general enjoyment" from 2 "old timers". James McCormick and Ren? J. Rene J Buesa wrote: Date: Fri, 1 Jun 2007 07:18:42 -0700 (PDT) From: Rene J Buesa Subject: RE: Re: [Histonet] processing fatty tissue To: "McCormick, James" CC: Doug Martin , "Drew N. Mehta" , Lamar Jones , Maxim Peshkov Dear Dr. McCormick: I could not have said it better! Your description of tissue components being GENTLY awashed by succesive flows of chemicals in low gradients of different chemical actions is what exactly exists in any procedure that intercalates between the different steps leading to the substitution of tissue water with the infiltrating medium. That is the rationale behind using EthOL at increasing concentrations from a "quite shocking" 60% start (could be gentler, although TAT imposes restrictions and limitations on "tissue processing gentleness"). Once the tissues have been "completely traumatized" at the end of the dehydration, the following "chemical shock" is given by the antemedium, usually the "infamous and noxious xylene" to end with the "soothing" effect of the paraffin. That is the rationale of my procedure of eliminating the harsh xylene effect with the combination of alcohols and "liquid paraffin" = mineral oil. In my procedure by mixing ethanol + isopropanol + mineral oil, and in Maxim's modification by mixing propanol and mineral oil only. Nothing new about using propanol, it has been in use to dehydrate since the early twentieth century, and now recently has been "resurrected" as the dehydrant of choice and sole chemical between the tissue water and the wax, as in the technology in use by the Peloris instrument which uses hight "instantaneous" temperatures to "dry-out" the tissue to eliminate the propanol and leave it "open" to the wax influx. I personally would never treat a tissue sample so harshly but you know how tissue preferences go, they are like beauty, all in the beholder's eyes. For me tissue processing should be gentle though. Being an "old timer" like me, you should remember the fantastic results we used to obtain when clearing tissue with Canada balsam, the precaution to cover the floating dehydrated tissue with a piece of filter paper moisted in ethanol to prevent the tissue to dry-out, and you should also remember that the tissue itself "said" when "I am ready for infiltration", after going to the bottom of the container, before being quickly washed with benzene. Remember those days? And also how long it took to infiltrate? And how soft they were to section? Again TAT chastised all of us and dictated quicker but NOT better procedures. Mixing alcohols and mineral oil, during protocols that take the same time as with other methods that include xylene, ease infiltration and help the histotech to section better, thinner and get rid of xylene as well. Just a thought you "provoqued" with your dancing image! Ren? J. "McCormick, James" wrote: Rene J. As I am familiar with much of the "antique" instruments and tissue processing methods............your writing helps me to understand the 1860's use of essential oils to dehydrate and clear tissue for "tallow" infiltration and later paraffin waxes.....one molecule after another "holds hands and changes partners" that we might call "the line dancing of tissue processing for histotechnology" . Kindest regards, Jim, J.B.McCormick,M.D. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, June 01, 2007 7:53 AM To: Maxim Peshkov; histonet@lists.utsouthwestern.edu Subject: Fwd: Re: [Histonet] processing fatty tissue Maxim: My answer to Robert without the attachment. Ren? J. Rene J Buesa wrote: Date: Fri, 1 Jun 2007 05:48:45 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] processing fatty tissue To: Robert Chiovetti Robert: The procedure is attached. The fundament is a GENTLE dehydrant substitution by the infiltrating agent (paraffin) and ELIMINATES the use of an antemedium (xylene). The general function of an antemedium is the ability to be the "connection" between the dehydrant and the wax (paraffin) because it mixes with both. Xylene (as well as white naphtha or some other aromatic chemicals) can do this, BUT the thing is that mineral oil (MO) IS paraffin of a low molecular weight so the antemedium is not needed or a mixture of MO with alcohols constitutes the antemedium. My procedure uses EthOL to dehydrate and later the antemedium is substituted by the mixture of EthOL + Isopropanol + MO Maxim has simplified the procedure because he process manually and don't have the advantage of vacuum or pressure or agitation as I did when developed the method. So it turns out that Maxim's modification is easier and more direct; he just dehydrates with propanol and later goes into the gentle substitution with a mixture of 5 parts of propanol + 1 part of MO heated at 50?C followed by another mixture of 2 parts of propanol + 1 of MO heated also at ?C to obtain the gentle and complete infiltration of ANY type of tissue. The infiltration with MO gives the tissues a softness never achieved with any other antemedium. You will see when you try it. Maxim's method is simpler than mine and, in the long run, will be more acceptable to all histotechs and also meets the objective of eliminating xylene from the histology lab. The procedure uses 2 chemicals that are cheaper and when needing to be disposed off, the propanol can be evaporated, the used MO mixed with used paraffin and both disposed off as a solid, cutting costs also in disposal. Try it, you will like it! Ren? J. Robert Chiovetti wrote: Maxim, Rene (and Other Histonetters), That's interesting re: using either a mix of ethanol+isopropanol+mineral oil (Rene) or isopropanol+mineral oil (Maxim) for breast tissue. Could you share your recipes with us? I have a customer (derm path) who could probably benefit from this for larger and thicker skin specimens which sometimes have a lot of subcutaneous fat associated with them. Thanks in advance, if you could share the recipes! Cheers, Bob Robert (Bob) Chiovetti, Ph.D. Southwest Precision Instruments The Desert Southwest's Microscopy Resource Tucson, Arizona USA Tel./Fax 520-546-4986 www.swpinet.com Member, Arizona Small Business Association (www.asba.com) ____________________________________________________________________________________ Be a PS3 game guru. Get your game face on with the latest PS3 news and previews at Yahoo! Games. http://videogames.yahoo.com/platform?platform=120121 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! oneSearch: Finally, mobile search that gives answers, not web links. --------------------------------- Building a website is a piece of cake. Yahoo! Small Business gives you all the tools to get online. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. --------------------------------- Ready for the edge of your seat? Check out tonight's top picks on Yahoo! TV. --------------------------------- Be a better Globetrotter. Get better travel answers from someone who knows. Yahoo! Answers - Check it out. From gcallis <@t> montana.edu Fri Jun 1 09:57:58 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Jun 1 10:00:13 2007 Subject: What kind of plastic? Re: [Histonet] Plastic embedding In-Reply-To: <01C7A456.E6CC7450.zumbor@email.cs.nsw.gov.au> References: <01C7A456.E6CC7450.zumbor@email.cs.nsw.gov.au> Message-ID: <6.0.0.22.1.20070601085621.01b51348@gemini.msu.montana.edu> Rosalba, What kind of plastic are you planning to use? Protocols vary for different plastics. At 10:12 PM 5/31/2007, you wrote: >Hi All, >Does anyone know of a reference source either a book or website which >explains plastic embedding from start to finish and all the parphenalia >required. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From dsprshospital <@t> yahoo.com Fri Jun 1 10:09:21 2007 From: dsprshospital <@t> yahoo.com (Des Peres Hospital) Date: Fri Jun 1 10:09:33 2007 Subject: [Histonet] Information about 2009 requirements Message-ID: <612114.5809.qm@web63707.mail.re1.yahoo.com> Hello All: I have been rather encapsulated for many years and out of the loop as far as the new requirements and have been hearing something about regs by 2009. Can anyone share with me what this is all about? I did know of the pathway to registry change that took affect a couple of years ago but nothing more really. Thanks, in advance. Susan --------------------------------- Fussy? Opinionated? Impossible to please? Perfect. Join Yahoo!'s user panel and lay it on us. From gcallis <@t> montana.edu Fri Jun 1 10:14:19 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Jun 1 10:14:04 2007 Subject: [Histonet] certification In-Reply-To: <34BB307EFC9A65429BBB49E330675F7201B0B78E@LTA3VS003.ees.hhs .gov> References: <34BB307EFC9A65429BBB49E330675F7201B0B78E@LTA3VS003.ees.hhs.gov> Message-ID: <6.0.0.22.1.20070601090718.01b6bd98@gemini.msu.montana.edu> Jeanine, Why, for hospital purposes to dictate salary? I am curious though, what would the Bachelor's degree be in? Anything? Did I start a Friday Flame It Up Discussion? If so, I apologize. At 03:44 AM 6/1/2007, you wrote: >All, > >While Jennifer is absolutely correct I must mention one issue. I know >of hospital labs that give their techs the title of HT or HTL based on >education (degree vs. non-degree) BUT requires no ASCP examination. >Basically, some hospitals simply bestow the title of HTL is you have a >Bachelor's and get trained on the job. If you do not have the degree >but they train you they give you the title of HT. So unless you see ASCP >after that HT or HTL, it may not mean what you think it does. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From POWELL_SA <@t> Mercer.edu Fri Jun 1 10:44:25 2007 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Fri Jun 1 10:46:05 2007 Subject: [Histonet] FW: Mealworm larvae In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E273C94@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <01MH9UOT8C8O000C0O@Macon2.Mercer.edu> Thanks so much for all your help. I am going to be ready when the mealworms arrive. Shirley _____ From: Monfils, Paul [mailto:PMonfils@Lifespan.org] Sent: Thursday, May 31, 2007 6:28 PM To: Shirley Powell Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] FW: Mealworm larvae The problems in sectioning mealworms are the same as in sectioning fruitflies and other arthropods, primarily the resistance of their chitinous integument to processing solvents and to sectioning. There are a few good methods for softening chitin which have been mentioned on Histonet previously. Perhaps you can find them in the archives by searching for "chitin". I haven't sectioned mealworms but I have successfully used one such method in sectioning fruitfly heads, which I will be glad to share if you can't locate the information. From gcallis <@t> montana.edu Fri Jun 1 11:09:47 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Jun 1 11:09:38 2007 Subject: Comments and usuage hints Re: [Histonet] ImmEdge pen and Menzel SuperFrost Plus slides In-Reply-To: <46601A4A.1010000@helsinki.fi> References: <46601A4A.1010000@helsinki.fi> Message-ID: <6.0.0.22.1.20070601093828.01b8c9f0@gemini.msu.montana.edu> Mirkka, I have tried them all, and on both the silane coated slides manufactured by Erie Scientific as Superfrost Plus Charge slides or their Polysine (poly l lysine) slides. We now use Vectors Immedge pens exclusively on any glass slide surface, coated or uncoated. In our hands, the DAKO pen, which may not be available in US now, peeled off in sheets, and the Super PAP pen wasn't ideal either. These pens resulted in a clear line of demarcation, but the Immedge pen has a hint of gray, making is much easier to see during staining. There used to be a super PAP pen with color, but the color leached under the coverslip over the sections. I am not sure this is available in the USA anymore either. The Immedge pen is also much cheaper, comes in a package of two. We are very happy with these pens. Hints on successful use: Vector said to vigorously shake ImmEdge pens before using them. This redistributes the goo and solvent inside the pen. I have heard the goo is teflon based, but cannot back up this fact since ingredients generally are proprietary. We vortex ImmEdge pens as hard as possible before drawing the lines. Vector made the agitation suggestion when people began to experience problems with their pens, and it works for us. I would assume shaking any vendors PAP type pen may improve their hydrophobic barrier effectiveness too. My biggest wish: Vendors and/or pen manufacturers, PLEASE make narrower pen tips and not out of such HARD material. My DAKO pen poured out goo like a fire hose resulting in goo flooded sections leading to very bad language habits. I finally crushed and macerated tips with a pliers. I have macerated the tips on other vendors pens too, and have recycled a favorite tip from one empty pan into the new pen - for a soft tip providing an even, minimal flow of goo when drawing the line! Good luck on finding a suitable hydrophobic barrier pen. At 07:08 AM 6/1/2007, you wrote: >Does anyone have any experience with using the ImmEdge or DAKO pens on >Menzel SuperFrost Plus adhesion slides? Vector doesn't really specify on >which glass slides the pen can be used, but some such as the Super PAP pen >and DAKO pen should be used only on albumin or polylysine coated slides >(according to the manufacturer, anyhow). I know of one group that uses the >DAKO pen succesfully on Superfrost Plus slides, but I'd like to have some >second opinions before making the purchase. Your help and expertise will >be greatly appreciated. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From lcastillos <@t> cogenics.com Fri Jun 1 12:17:17 2007 From: lcastillos <@t> cogenics.com (Castillos, Luminita) Date: Fri Jun 1 12:17:25 2007 Subject: [Histonet] DNA extraction method from archival Giemsa stained bone marrow smears Message-ID: Hi everyone, Does anyone have any experience with using a good method to extract DNA from archival Giemsa stained bone marrow smears?. Is there available a commercial kit for the DNA extraction?. Thank you in advance and have a great Friday. Sincerely, Luminita From mpence <@t> grhs.net Fri Jun 1 12:54:56 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Fri Jun 1 12:55:04 2007 Subject: [Histonet] FW: Clinical Use of CoPath Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C610@IS-E2K3.grhs.net> -----Original Message----- From: Mike Pence Sent: Friday, June 01, 2007 12:33 PM To: histonet-bounces@lists.utsouthwestern.edu Subject: Clinical Use of CoPath I was wondering if any of you in the Clinical World are using CoPath as your computer software and if you are, do you use it with barcode readers? Thanks, Mike From MSHERWOOD <@t> PARTNERS.ORG Fri Jun 1 13:02:28 2007 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Fri Jun 1 13:03:26 2007 Subject: [Histonet] DNA extraction method from archival Giemsa stained bonemarrow smears In-Reply-To: Message-ID: <1AF23D0AD12E7444A5DB083CA978B73407A31231@PHSXMB1.partners.org> Luminita, I refer you to Molecular Devices-Arcturus Division. They sell the PicoPure DNA extraction kit--it is used in conjunction with the Paradise Reagent Kit for FFPE tissue. You can either use it for laser capture microdissection (LCM) or tissue you have scraped off the slide. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Castillos, Luminita Sent: Friday, June 01, 2007 1:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DNA extraction method from archival Giemsa stained bonemarrow smears Hi everyone, Does anyone have any experience with using a good method to extract DNA from archival Giemsa stained bone marrow smears?. Is there available a commercial kit for the DNA extraction?. Thank you in advance and have a great Friday. Sincerely, Luminita _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From anh2006 <@t> med.cornell.edu Fri Jun 1 14:03:04 2007 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Fri Jun 1 14:03:20 2007 Subject: [Histonet] ImmEdge pen and Menzel SuperFrost Plus slides In-Reply-To: <6.0.0.22.1.20070601093828.01b8c9f0@gemini.msu.montana.edu> References: <46601A4A.1010000@helsinki.fi> <6.0.0.22.1.20070601093828.01b8c9f0@gemini.msu.montana.edu> Message-ID: [Disclaimer: I haven't used Menzel SuperFrost Plus slides but use VWR with great success.] I love the Vector ImmEdge pen, it is my favorite. My facvorite used to be Zymed's as it was thinner and stayed on the slide no matter what I did but they must have changed their formula as I don't like it anymore and it comes off the slide now. I find the trick is you must gently blow on the pap penned area for a few seconds before putting back in PBS - to dry the pap-pen so to speak. Otherwise it comes off. > >At 07:08 AM 6/1/2007, you wrote: >>Does anyone have any experience with using the ImmEdge or DAKO pens >>on Menzel SuperFrost Plus adhesion slides? Vector doesn't really >>specify on which glass slides the pen can be used, but some such as >>the Super PAP pen and DAKO pen should be used only on albumin or >>polylysine coated slides (according to the manufacturer, anyhow). I >>know of one group that uses the DAKO pen succesfully on Superfrost >>Plus slides, but I'd like to have some second opinions before >>making the purchase. Your help and expertise will be greatly >>appreciated. > -- From lpwenk <@t> sbcglobal.net Fri Jun 1 16:39:24 2007 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Fri Jun 1 16:39:39 2007 Subject: [Histonet] certification In-Reply-To: <000001c7a448$7e43c680$d00f7ca5@lurie.northwestern.edu> Message-ID: <000701c7a495$53432460$0202a8c0@HPPav2> Just clarification - On-the-job (OJT) training for HT was NOT removed from the BOR certification. It is still possible for people to take the ASCP BOR HT exam via the OJT route. Previous to Jan. 2005, there were two (2) OJT routes. - high school diploma + minimum 2 years full time OJT experience in histology - associate degree (or minimum of 60 credit hours) with 12 credits minimum in biology and chemistry + minimum 1 year full time OJT experience in histology After Jan. 2005, the high school route was eliminated. The associate degree OJT route is still available. In addition, there is the route via a NAACLS-accredited histology program, which can have prerequisit requirements anywhere from a high school diploma through an associate degree. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Friday, June 01, 2007 8:29 AM To: 'Bartlett, Jeanine (CDC/CCID/NCZVED)'; 'Jennifer MacDonald'; 'Emily Sours' Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] certification All, Just remember as of January 2005 the on-the-job training for HT was removed from BOR certification pathway. I don't know of anyone bestowed a title just by education (fortunately) I do have issues with uncertified people doing histo with no knowledge of what they are actually doing and belittling the fact that I made an effort to get my HTL. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Friday, June 01, 2007 4:45 AM To: Jennifer MacDonald; Emily Sours Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] certification All, While Jennifer is absolutely correct I must mention one issue. I know of hospital labs that give their techs the title of HT or HTL based on education (degree vs. non-degree) BUT requires no ASCP examination. Basically, some hospitals simply bestow the title of HTL is you have a Bachelor's and get trained on the job. If you do not have the degree but they train you they give you the title of HT. So unless you see ASCP after that HT or HTL, it may not mean what you think it does. Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Friday, June 01, 2007 2:08 AM To: Emily Sours Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] certification Emily, The ASCP (American Society for Clinical Pathology) certifies laboratory personnel. This is a national certification, recognized by all states, although there are some states that require a license in addition. In order to become certified you are required to take an exam. To be eligible for the HT certification examination, you must have an AS degree and one year of experience in a histology laboratory or graduate from a NAACLS accredited program. For the HTL level certification a BS degree is required and one year experience, or the BS degree and graduation from a NAACLS accredited program. Visit www.ascp.org and go to the Board of Registry (BOR) area Should you have any other questions please feel free to contact me. Jennifer Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu "Emily Sours" Sent by: histonet-bounces@lists.utsouthwestern.edu 05/31/2007 10:07 PM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] certification What exactly makes one a certified HT or HTL tech? Does this vary by state? I've never heard of it and googling it isn't helping. (my apologies to Greg Luck for the multiple emails!) Emily -- Pray, v.: To ask that the laws of the universe be annulled on behalf of a single petitioner confessedly unworthy. -Ambrose Bierce _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Maxim_71 <@t> mail.ru Sat Jun 2 04:12:34 2007 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Sat Jun 2 04:14:53 2007 Subject: [Histonet] processing fatty tissue Message-ID: <473331975.20070602131234@mail.ru> Robert: In my protocol I used graduate alcohols (isopropanol) for dehydratation, then mixtures isopropanol:mineral oil 5:1 and 1:2 at 50oC, then mineral oil instead xylene. Paraffin's infiltration as usual. These reagents give the "soft" transitions from dehydratant to paraffin. Difficult tissues will cut more easily. Risk of inhalations toxic chemicals decreased to 5 times. If I had VIP processor and 100% ethanol, that I simple used Rene's protocol. My protocol based on protocol Buesa (RJ Buesa, JOH/Vol.23, ?2/June 2000. Mineral Oil: The Best Xylene Substiute for Tissue Processing Yet?). 1 Isopropanol @70% 1h RT 2 Isopropanol @80% 1h RT 3 Isopropanol @90% 1h RT 4 Isopropanol @92% 1h RT 5 Isopropanol 99% (fresh) 1h RT 6 IM 5:1 1.5h 50?? 7 IM 4:2 1.5h 50?? 8 Mineral oil 50?? overnight 9 Paraffin 1h 60?? 10 Paraffin 1h 60?? 11 Paraffin 1h 60?? 12 Paraffin 1h 60?? Now we routinely process all surgical specimens include bone, eyeball, uterus etc with it. I hope this protocol would like to you. Sincerely, Maxim Peshkov Russia Taganrog. From bbroders <@t> unlnotes.unl.edu Sat Jun 2 06:58:11 2007 From: bbroders <@t> unlnotes.unl.edu (Bruce W Brodersen) Date: Sat Jun 2 06:58:15 2007 Subject: [Histonet] Histology Laboratory Manager Message-ID: HISTOLOGY LAB MANAGER University of Nebraska?Lincoln Veterinary Diagnostic Center, Department of Veterinary and Biomedical Sciences Manage operations of a busy, well equipped modern histology laboratory located on East Campus. The lab provides daily (M-F) histologic services to support veterinary diagnostic pathology and research. Responsible to supervise 4-6 full time staff and manage operations, including work scheduling, workload recording, inventory, equipment maintenance, archives and data. Associate's or equivalent education/experience plus Histotechnician (HT, ASCP) certification and five years histotechnician experience. A thorough knowledge of histologic techniques, including fixation and staining procedures essential. Bachelor's degree, Histotechnologist (HTL, ASCP) certification, supervisory experience and understanding of immunohistochemistry preferred. Excellent benefits including staff/dependent scholarship program. Review of applications will begin June 15th See Requisition 070451 at http://employment.unl.edu for complete description of duties, qualification requirements and to apply for the position. UNL is committed to AA/EEO and ADA/504. If you require an accommodation, please call 402-472-1412. Bruce W. Brodersen, DVM, PhD University of Nebraska Veterinary Diagnostic Center Fair St. & E. Campus Loop Lincoln, NE 68583-0907 voice (402) 472-1434 FAX (402 472-3094 From Laurie <@t> conxis.com Sat Jun 2 13:39:22 2007 From: Laurie <@t> conxis.com (Laurie Popp) Date: Sat Jun 2 13:39:23 2007 Subject: [Histonet] Re: Clinical use of Co-path Message-ID: <002c01c7a545$569aa3d0$9700a8c0@laurie> Hi Mike, We use CoPath with barcode readers in our buttoning area at Mayo in Rochester. The only area in our lab that we are currently using barcodes is our buttoning/gross description area. Laurie Popp HT Candidate Mayo Clinic -----Original Message----- From: Mike Pence Sent: Friday, June 01, 2007 12:33 PM To: histonet-bounces@lists.utsouthwestern.edu Subject: Clinical Use of CoPath I was wondering if any of you in the Clinical World are using CoPath as your computer software and if you are, do you use it with barcode readers? Thanks, Mike From kzhong888 <@t> yahoo.com Sat Jun 2 17:39:24 2007 From: kzhong888 <@t> yahoo.com (dfs dsaf) Date: Sat Jun 2 17:39:29 2007 Subject: [Histonet] Old equipment Message-ID: <673420.35583.qm@web52907.mail.re2.yahoo.com> Hello Histonetters: I am looking for a couple pieces of old equipment. One is a IEC CTD-Harris Cryostat. It is the smaller ice cream cart type cryostat. It is about 30 years old is out of production. I am also looking for a Technicon mono or duo tissue processors, model 2A. These are the carousel style processors that were very popular 40-60 years ago. Any one with any leads please email me at Kzhong888@yahoo.com Thank you Kirk ZHong --------------------------------- Choose the right car based on your needs. Check out Yahoo! Autos new Car Finder tool. From kzhong888 <@t> yahoo.com Sat Jun 2 17:40:32 2007 From: kzhong888 <@t> yahoo.com (dfs dsaf) Date: Sat Jun 2 17:40:37 2007 Subject: [Histonet] Looking for Old IEC CTD harris cryostat and Technicon processor. Message-ID: <986626.36606.qm@web52907.mail.re2.yahoo.com> Hello Histonetters: I am looking for a couple pieces of old equipment. One is a IEC CTD-Harris Cryostat. It is the smaller ice cream cart type cryostat. It is about 30 years old is out of production. I am also looking for a Technicon mono or duo tissue processors, model 2A. These are the carousel style processors that were very popular 40-60 years ago. Any one with any leads please email me at Kzhong888@yahoo.com Thank you Kirk ZHong --------------------------------- Yahoo! oneSearch: Finally, mobile search that gives answers, not web links. From nfournier <@t> sasktel.net Sat Jun 2 21:18:30 2007 From: nfournier <@t> sasktel.net (Neil Fournier) Date: Sat Jun 2 21:18:35 2007 Subject: [Histonet] long-term storage of rat brain at -80 Message-ID: <000f01c7a585$7a71f2f0$07ffc5d8@NEIL> I thought many of you might able to help me devise a protocol for long-term storage of flash-frozen perfused rat brains. I am sure what I am doing currently is not appropriate. Our procedure thus far is to perfuse rats with saline followed by 4% paraformaldehyde. Post-fix for 48 h and then cryoprotect in 30% sucrose until the brain sinks. The brains are then removed from sucrose and dried with a Kim Wipe, blocked in the coronal plane, and placed in cryomolds containing OCT. The mold is then frozen in liquid nitrogen cooled isopentane. The frozen mold is then placed in a empty ziplock bag and placed in the -80 freezer. Several months after I did this procedure and sectioned the brains, I found significant holes indicative of freezing artifact in the tissue. Although I have had some problems in the past with my isopentane freezing protocol, I have a feeling that this issue is probably the result of storage at -80 degree C but I cannot be for certain. (I do not believe the issue is perfusion based since we have no issues with microtome sectioned or vibratome sectioned tissue for IHC). Could anyone share with me their protocol or provide suggestions? Lastly, I have looked high and low but does anyone know where I can find some long plastic forceps that would be of adequate length for flash freezing? Our current one was broken accidentally and none of us know where it came from although we are now beginning to suspect that there might be a mystical histofairy who is responsible for the often magically appearing (and sometimes disappearing) histological chemicals and equipment in labs. Thanks again, Neil From dfinkelstein <@t> mhri.edu.au Sun Jun 3 18:00:11 2007 From: dfinkelstein <@t> mhri.edu.au (David Finkelstein) Date: Sun Jun 3 17:52:59 2007 Subject: [Histonet] long-term storage of rat brain at -80 Message-ID: <000001c7a632$f0893d10$2128a8c0@mhri.edu.au> Dear Neil It sounds like freeze fracture not a storage problem. Long tem freezing causes freeze drying not holes. Two suggestions. 1) Get rid the OCT. Ether suspend the brain in the mold just above the liquid nitrogen or drop the brain into the isopentane for no more than 20 sec. Then place the brain on dry ice. Keep the plastic bags on dry ice. Wrap the brains plastic file kept on dry ice. Then put them in the bags. Always transport them in or on dry ice. 2) use metal forceps. Keep the tips in dry ice. Good luck David Assoc. Professor David Finkelstein, The Mental Health Research Institute of Victoria, 155 Oak Street, Parkville, Victoria 3052 AUSTRALIA dfinkelstein@mhri.edu.au Message: 4 Date: Sat, 02 Jun 2007 20:18:30 -0600 From: Neil Fournier Subject: [Histonet] long-term storage of rat brain at -80 To: histonet@lists.utsouthwestern.edu Message-ID: <000f01c7a585$7a71f2f0$07ffc5d8@NEIL> Content-Type: text/plain; charset=iso-8859-1 I thought many of you might able to help me devise a protocol for long-term storage of flash-frozen perfused rat brains. I am sure what I am doing currently is not appropriate. Our procedure thus far is to perfuse rats with saline followed by 4% paraf ormaldehyde. Post-fix for 48 h and then cryoprotect in 30% sucrose until the brain sinks. The brains are then removed from sucrose and dried with a Kim Wipe, blocked in the coronal plane, and placed in cryomolds containing OCT. The mold is then frozen in liquid nitrogen cooled isopentane. The frozen mold is then placed in a empty ziplock bag and placed in the -80 freezer. Several months after I did this procedure and sectioned the brains, I found significant holes indicative of freezing artifact i n the tissue. Although I have had some problems in the past with my isopentane freezing protocol, I have a feeling that this issue is probably the result of storage at -80 degree C but I cannot be for certain. (I do not believe the issue is perfusio n based si Could anyone share with me their protocol or provide suggestions? Lastly, I have looked high and low but does anyone know where I can find some long plastic forceps that would be of adequate length for flash freezing? Our current one was broken accide ntally and none of us know where it came from although we are now beginning to suspect that there might be a mystical histofairy who is responsible for the often magically appearing (and sometimes disappearing) histological chemicals and equipment in la bs. Thanks again, Neil ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 43, Issue 4 *************************************** From webb3655 <@t> sbcglobal.net Sun Jun 3 19:36:22 2007 From: webb3655 <@t> sbcglobal.net (judy webb) Date: Sun Jun 3 19:36:27 2007 Subject: [Histonet] Out sourcing transcription Message-ID: <92048.1866.qm@web83610.mail.sp1.yahoo.com> Could someone from Swedish Hospital System in Denver Co. contact me off line? Thanks, Judy Webb McKinney JPS Health Network Fort Worth, Texas jwebb01@jpshealthnetwork.org From talulahgosh <@t> gmail.com Mon Jun 4 09:01:53 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Mon Jun 4 09:01:59 2007 Subject: [Histonet] long-term storage of rat brain at -80 In-Reply-To: <000001c7a632$f0893d10$2128a8c0@mhri.edu.au> References: <000001c7a632$f0893d10$2128a8c0@mhri.edu.au> Message-ID: While we don't flash freeze our embryos, we do fix in para and sink in 30% sucrose like you do. Then, instead of using just OCT to embed, we use a 1:1 mix of OCT and 30% sucrose. This helps when sectioning, if that seems to be the problem. I'm not sure if this solution will work with fast freezing, we freeze our embedded embryos on dry ice. Emily -- Pray, v.: To ask that the laws of the universe be annulled on behalf of a single petitioner confessedly unworthy. -Ambrose Bierce From shive003 <@t> umn.edu Mon Jun 4 09:53:22 2007 From: shive003 <@t> umn.edu (Jan Shivers) Date: Mon Jun 4 09:53:27 2007 Subject: [Histonet] research labs only - serial sectioning pricing Message-ID: <002d01c7a6b8$195124d0$a1065486@auxs.umn.edu> Hello, What/how are research/service Histo labs charging for multiple serial sections on research material (meaning, charged to a research budget and not a patient's insurance). Do you charge the same price for every single serial section taken off a block, or is the first slide charged at full recut price, and the subsequent slides are at a reduced rate? Thanks in advance. Jan Shivers UMN Vet Diag Lab From cwscouten <@t> myneurolab.com Mon Jun 4 10:55:39 2007 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Mon Jun 4 10:54:59 2007 Subject: [Histonet] long-term storage of rat brain at -80 References: <000001c7a632$f0893d10$2128a8c0@mhri.edu.au> Message-ID: <5784D843593D874C93E9BADCB87342AB03507297@tpiserver03.Coretech-holdings.com> Freeze fracture is lines through the tissue parallel to the blade passage. The "swiss cheese" holes are distinctly different. Neil, see page 48 of the January 2007 issue of Microscopy today, or the following link, for a full discussion of your problem: http://www.myneurolab.com/global/Manuals/Tips%20and%20Techniques%20Freezing%20Artifact.pdf Fast freezing prevents crystal formation and expansion of the water in tissue. Expansion causes the 'swiss cheese'. However, the vitreous ice formed by fast freezing is an unstable state of nature. At any temperature above something like -200C, it gradually reforms as crystaline ice. The less cold, the faster the reformation. Did you leave it in the cyrostat overnight to get to cutting temperature? Long term frozen organs will gradually develop freezing artifact. No way around it except maybe liquid helium or other outragously expensive storage. You only solution would be cut earlier, don't store so long. Or keep colder at all times, and cut as soon as you reach a warm enough temperature to cut. The 30% sucrose should help some, but you are already doing that. Cordially, Charles W.? Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 x 342 FAX? 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David Finkelstein Sent: Sunday, June 03, 2007 6:00 PM To: histonet@lists.utsouthwestern.edu Cc: nfournier@sasktel.net Subject: [Histonet] long-term storage of rat brain at -80 Dear Neil It sounds like freeze fracture not a storage problem. Long tem freezing causes freeze drying not holes. Two suggestions. 1) Get rid the OCT. Ether suspend the brain in the mold just above the liquid nitrogen or drop the brain into the isopentane for no more than 20 sec. Then place the brain on dry ice. Keep the plastic bags on dry ice. Wrap the brains plastic file kept on dry ice. Then put them in the bags. Always transport them in or on dry ice. 2) use metal forceps. Keep the tips in dry ice. Good luck David Assoc. Professor David Finkelstein, The Mental Health Research Institute of Victoria, 155 Oak Street, Parkville, Victoria 3052 AUSTRALIA dfinkelstein@mhri.edu.au Message: 4 Date: Sat, 02 Jun 2007 20:18:30 -0600 From: Neil Fournier Subject: [Histonet] long-term storage of rat brain at -80 To: histonet@lists.utsouthwestern.edu Message-ID: <000f01c7a585$7a71f2f0$07ffc5d8@NEIL> Content-Type: text/plain; charset=iso-8859-1 I thought many of you might able to help me devise a protocol for long-term storage of flash-frozen perfused rat brains. I am sure what I am doing currently is not appropriate. Our procedure thus far is to perfuse rats with saline followed by 4% paraf ormaldehyde. Post-fix for 48 h and then cryoprotect in 30% sucrose until the brain sinks. The brains are then removed from sucrose and dried with a Kim Wipe, blocked in the coronal plane, and placed in cryomolds containing OCT. The mold is then frozen in liquid nitrogen cooled isopentane. The frozen mold is then placed in a empty ziplock bag and placed in the -80 freezer. Several months after I did this procedure and sectioned the brains, I found significant holes indicative of freezing artifact i n the tissue. Although I have had some problems in the past with my isopentane freezing protocol, I have a feeling that this issue is probably the result of storage at -80 degree C but I cannot be for certain. (I do not believe the issue is perfusio n based si Could anyone share with me their protocol or provide suggestions? Lastly, I have looked high and low but does anyone know where I can find some long plastic forceps that would be of adequate length for flash freezing? Our current one was broken accide ntally and none of us know where it came from although we are now beginning to suspect that there might be a mystical histofairy who is responsible for the often magically appearing (and sometimes disappearing) histological chemicals and equipment in la bs. Thanks again, Neil ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 43, Issue 4 *************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jstaruk <@t> masshistology.com Mon Jun 4 14:07:31 2007 From: jstaruk <@t> masshistology.com (Jim Staruk) Date: Mon Jun 4 14:07:31 2007 Subject: [Histonet] research labs only - serial sectioning pricing In-Reply-To: <002d01c7a6b8$195124d0$a1065486@auxs.umn.edu> References: <002d01c7a6b8$195124d0$a1065486@auxs.umn.edu> Message-ID: <000e01c7a6db$9b33f270$4001a8c0@FrontOffice> Jan, We charge $5.75 for the first H&E-stained slide (this includes tissue processing and paraffin embedding) and then $2.50 for each additional H&E-stained slide. Jim ______________________ Jim Staruk Mass Histology Service www.masshistology.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jan Shivers Sent: Monday, June 04, 2007 10:53 AM To: histonet Subject: [Histonet] research labs only - serial sectioning pricing Hello, What/how are research/service Histo labs charging for multiple serial sections on research material (meaning, charged to a research budget and not a patient's insurance). Do you charge the same price for every single serial section taken off a block, or is the first slide charged at full recut price, and the subsequent slides are at a reduced rate? Thanks in advance. Jan Shivers UMN Vet Diag Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From drkwolfe <@t> telus.net Mon Jun 4 14:10:08 2007 From: drkwolfe <@t> telus.net (Joseph Kapler) Date: Mon Jun 4 14:10:16 2007 Subject: [Histonet] Looking for books needing a new home Message-ID: Good day Y'all I'm currently studying from my BSc in Histo Tech. At the same time, I am working in a histology research lab (a dream job considering my path of studies). The boss expects me to stretch my level of understanding to the breaking point, knowing that I can learn better when under pressure, or the best adage of on the job training. In my case, the training is secondary to my schooling, not the other way around. The basis of my post... I am looking for some additional research text books to give me a step ahead. Two books I am looking for, and would like to keep a copy for future use too. It would be great if any of the texts can be donated to a good cause (my education and the education of those that come after myself), however, I'm willing to pay for additional texts where feasible. Theory and Practice of Histological Techniques 5th Edition, Edited by John Bancroft and Marilyn Gamble Textbook of Histology 1st edition Finn Geneser 1986 Munksgaard, Copenhagen Lynch's Medical Laboratory Technology (3rd edition or better) Vol 1 or Volume 1 and 2 if 4th edition And finally any textbooks relating to Vet Medicine, Zoology, Gross Human Anatomy, and any atlases for mouse, rat, human compatable structures, etc. If you have any of these books, or any others that relate to histology and med lab sciences, especially those that cover many of the older techniques and styles that may not be used as much in this day. Anything that can help woould be great. And I thank everyone in advance, both for the consideration of the books, and for all the great advice I have received just from my membership on the list. And this book, if a copy is available anywhere in the free world, I would be willing to pay for this book. The actual amount, would be up for bargaining. Histological Techniques - Manifred Gabe Laboratoire d'Evolution, Facultedes Sciences, Paris France Translated by Robert E. Blackith Published: Masson 1979 Springer-Verlag Again, thanks Joe Kapler Edmonton Canada From vazquezr <@t> ohsu.edu Mon Jun 4 14:24:53 2007 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Mon Jun 4 14:25:20 2007 Subject: [Histonet] Question about taking test Message-ID: Hello, I have a question to ask. I have a co-worker that is interested in taking the test and has a B.S. We work in a Mohs lab, but not an actual histology lab where we would do special stains. She has been here almost 4 years doing mostly Mohs surgeries, very little paraffin processing. As I am understanding it, is that she would have to have 1 year experience in a histology lab doing special stains and paraffin cutting and processing? Since they don't make you do special stains and send them in, then she would have to prove that she can perform a special stain and that is where the 1 year experience comes in? Thanks Robyn OHSU From bhewlett <@t> cogeco.ca Mon Jun 4 14:37:53 2007 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Mon Jun 4 14:37:56 2007 Subject: [Histonet] Looking for books needing a new home References: Message-ID: <003001c7a6df$d8608330$6500a8c0@mainbox> Joe, These and many other useful texts may be obtained from the following Canadian site; http://www.abebooks.com/ Regards, Bryan ----- Original Message ----- From: "Joseph Kapler" To: "Histonet Listserve" Sent: Monday, June 04, 2007 3:10 PM Subject: [Histonet] Looking for books needing a new home > Good day Y'all > > I'm currently studying from my BSc in Histo Tech. At the same time, I am > working in a histology research lab (a dream job considering my path of > studies). The boss expects me to stretch my level of understanding to the > breaking point, knowing that I can learn better when under pressure, or > the > best adage of on the job training. In my case, the training is secondary > to > my schooling, not the other way around. > > The basis of my post... I am looking for some additional research text > books > to give me a step ahead. Two books I am looking for, and would like to > keep > a copy for future use too. It would be great if any of the texts can be > donated to a good cause (my education and the education of those that come > after myself), however, I'm willing to pay for additional texts where > feasible. > > Theory and Practice of Histological Techniques 5th Edition, > Edited by John Bancroft and Marilyn Gamble > > Textbook of Histology 1st edition > Finn Geneser > 1986 Munksgaard, Copenhagen > > Lynch's Medical Laboratory Technology (3rd edition or better) Vol 1 > or Volume 1 and 2 if 4th edition > > And finally any textbooks relating to Vet Medicine, Zoology, Gross Human > Anatomy, and any atlases for mouse, rat, human compatable structures, etc. > > If you have any of these books, or any others that relate to histology and > med lab sciences, especially those that cover many of the older techniques > and styles that may not be used as much in this day. Anything that can > help > woould be great. > > And I thank everyone in advance, both for the consideration of the books, > and for all the great advice I have received just from my membership on > the > list. > > And this book, if a copy is available anywhere in the free world, I would > be > willing to pay for this book. The actual amount, would be up for > bargaining. > > Histological Techniques - Manifred Gabe > Laboratoire d'Evolution, Facultedes Sciences, Paris France > Translated by Robert E. Blackith > Published: Masson 1979 > Springer-Verlag > > Again, thanks > > Joe Kapler > Edmonton Canada > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From KMENDELL <@t> nhs-healthlink.org Mon Jun 4 14:53:35 2007 From: KMENDELL <@t> nhs-healthlink.org (Kate Mendell) Date: Mon Jun 4 14:53:58 2007 Subject: [Histonet] Biopsy Bags Message-ID: <4664357F020000A800015DD2@mail.NHS-HEALTHLINK.ORG> Does anyone know where you can still get the tea biopsy bags. Fisher no longer carries them and most others that I have found have gone to the nylon bags. We have tried those bags and don't care for them. If someone knows who is still selling the tea bag biopsy could you please send me the information Thanks Kate This message and its contents are confidential and are intended for the use of the addressee only, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the intended recipient, this serves as notice that any unauthorized distribution, duplication, printing, or any other use is strictly prohibited. If you feel you have received this email in error, please delete the message and notify the sender so that we may prevent future occurrences. From gcallis <@t> montana.edu Mon Jun 4 16:00:08 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Jun 4 15:59:49 2007 Subject: [Histonet] paper tea bags for biopsies, a source Re: Biopsy bags In-Reply-To: <4664357F020000A800015DD2@mail.NHS-HEALTHLINK.ORG> References: <4664357F020000A800015DD2@mail.NHS-HEALTHLINK.ORG> Message-ID: <6.0.0.22.1.20070604144326.01b4b3c0@gemini.msu.montana.edu> Kate, I found this website, and it was a winner for our beloved tea bags - they also had muslin bags, and they sell to tea distributors!! http://www.sunburstbottle.com/bags/tea-muslin I suggest you buy them in the biggest lot size to stay safe with supply. It is sad when vendors discontinue little items like this in favor of what? Stiff nylon bags that flatten the biopsy, or are hard to handle, ugh!! We are not happy with the nylon bags either, but do use them for decalcifying larger bones. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From HornHV <@t> archildrens.org Mon Jun 4 16:39:38 2007 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Mon Jun 4 16:39:56 2007 Subject: [Histonet] paper tea bags for biopsies, a source Re: Biopsy bags In-Reply-To: <6.0.0.22.1.20070604144326.01b4b3c0@gemini.msu.montana.edu> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB7180@EMAIL.archildrens.org> Thanks Gail I have filed this away for the future. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Monday, June 04, 2007 4:00 PM To: Kate Mendell; Histonet@lists.utsouthwestern.edu Subject: [Histonet] paper tea bags for biopsies, a source Re: Biopsy bags Kate, I found this website, and it was a winner for our beloved tea bags - they also had muslin bags, and they sell to tea distributors!! http://www.sunburstbottle.com/bags/tea-muslin I suggest you buy them in the biggest lot size to stay safe with supply. It is sad when vendors discontinue little items like this in favor of what? Stiff nylon bags that flatten the biopsy, or are hard to handle, ugh!! We are not happy with the nylon bags either, but do use them for decalcifying larger bones. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From lpwenk <@t> sbcglobal.net Mon Jun 4 17:06:57 2007 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Mon Jun 4 17:07:22 2007 Subject: [Histonet] Question about taking test In-Reply-To: Message-ID: <000901c7a6f4$abf8aa60$0202a8c0@HPPav2> There are two parts to qualifying to take the HTL exam. First, the BS degree with 30 hours of biology AND chemistry combined plus a minimum of 1 year's experience within the last 10 years under pathologist certified by the American Board of Pathology in Anatomic pathology (or the equivalent of a PhD. Second, the experience must be within the last 10 years, and cover fixation, microtomy, processing and staining. There are no other specifications. So the microtomy CAN be just frozen sections (or just plastics, or just paraffin, etc.). So the staining CAN be just H&E. (BTW, This experience criteria is the same for the HT exam.) Now, with that said, the exams WILL have questions covering ALL aspects of histotechnology - fixation, processing, decalcification, microtomy (frozen and paraffin), all special stains, IHC, tissue ID for all tissues, enzymes on the HTL, safety, equipment, lab math, etc. So anyone who works in just one specialty area (Mohs, derm, GI, etc.), will be at a disadvantage. They will need a lot of books, atlases, and a lot of time to learn all that is required. They can pass, but it will take a lot more work than someone who routinely sees a variety of tissues and does a variety of special stains. The last two cycles of HTL exams, 51% and 70% of applicants were able to pass the HTL exam. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robyn Vazquez Sent: Monday, June 04, 2007 3:25 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Question about taking test Hello, I have a question to ask. I have a co-worker that is interested in taking the test and has a B.S. We work in a Mohs lab, but not an actual histology lab where we would do special stains. She has been here almost 4 years doing mostly Mohs surgeries, very little paraffin processing. As I am understanding it, is that she would have to have 1 year experience in a histology lab doing special stains and paraffin cutting and processing? Since they don't make you do special stains and send them in, then she would have to prove that she can perform a special stain and that is where the 1 year experience comes in? Thanks Robyn OHSU _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Laurie <@t> conxis.com Mon Jun 4 17:08:11 2007 From: Laurie <@t> conxis.com (Laurie Popp) Date: Mon Jun 4 17:08:12 2007 Subject: [Histonet] Re: Clinical use of Co-path In-Reply-To: <46642311.1030507@pathology.washington.edu> References: <002c01c7a545$569aa3d0$9700a8c0@laurie> <46642311.1030507@pathology.washington.edu> Message-ID: <003701c7a6f4$d7dfade0$9700a8c0@laurie> Buttoning for us is what the rest of the world calls grossing. Laurie -----Original Message----- From: Victor Tobias [mailto:victor@pathology.washington.edu] Sent: Monday, June 04, 2007 9:35 AM To: Laurie Popp Subject: Re: [Histonet] Re: Clinical use of Co-path What is buttoning? Laurie Popp wrote: >Hi Mike, > >We use CoPath with barcode readers in our buttoning area at Mayo in >Rochester. The only area in our lab that we are currently using >barcodes is our buttoning/gross description area. > >Laurie Popp >HT Candidate >Mayo Clinic > >-----Original Message----- >From: Mike Pence >Sent: Friday, June 01, 2007 12:33 PM >To: histonet-bounces@lists.utsouthwestern.edu >Subject: Clinical Use of CoPath > > >I was wondering if any of you in the Clinical World are using CoPath as >your computer software and if you are, do you use it with barcode >readers? > >Thanks, >Mike > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From HornHV <@t> archildrens.org Mon Jun 4 17:10:42 2007 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Mon Jun 4 17:10:52 2007 Subject: [Histonet] Re: Clinical use of Co-path In-Reply-To: <003701c7a6f4$d7dfade0$9700a8c0@laurie> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB7182@EMAIL.archildrens.org> The first clinical histology job I had one of the pathologist called cassettes, buttons. I had never heard that before. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Popp Sent: Monday, June 04, 2007 5:08 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Clinical use of Co-path Buttoning for us is what the rest of the world calls grossing. Laurie -----Original Message----- From: Victor Tobias [mailto:victor@pathology.washington.edu] Sent: Monday, June 04, 2007 9:35 AM To: Laurie Popp Subject: Re: [Histonet] Re: Clinical use of Co-path What is buttoning? Laurie Popp wrote: >Hi Mike, > >We use CoPath with barcode readers in our buttoning area at Mayo in >Rochester. The only area in our lab that we are currently using >barcodes is our buttoning/gross description area. > >Laurie Popp >HT Candidate >Mayo Clinic > >-----Original Message----- >From: Mike Pence >Sent: Friday, June 01, 2007 12:33 PM >To: histonet-bounces@lists.utsouthwestern.edu >Subject: Clinical Use of CoPath > > >I was wondering if any of you in the Clinical World are using CoPath as >your computer software and if you are, do you use it with barcode >readers? > >Thanks, >Mike > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From mickie25 <@t> netzero.net Mon Jun 4 17:47:49 2007 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Mon Jun 4 17:48:17 2007 Subject: [Histonet] Re: Clinical use of Co-path In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB7182@EMAIL.archildrens.org> References: <003701c7a6f4$d7dfade0$9700a8c0@laurie> <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB7182@EMAIL.archildrens.org> Message-ID: Hi All, Buttons refer to the round (and sometimes square) metal cassettes with snap lids which were commonly used back in the mono and duo Technicon days. This was near the dinosaur era about 30 - 35 or so years ago. Later came plastic cassettes and little metal molds made by Tissue Tek. I remember interviewing for a job at the Los Angeles County General Hospital in 1973 and they still used lead L-molds and had the days supply stacked up about two feet high beside the embedding area. They used a heated pitcher to fill the molds. I thought that was archaic in those days because there were peel-a-way molds and the Tissue Tek embedding center which worked 'better'. Well, so much for stimulating that part of my memory. Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Monday, June 04, 2007 3:11 PM To: Laurie Popp; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Clinical use of Co-path The first clinical histology job I had one of the pathologist called cassettes, buttons. I had never heard that before. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Popp Sent: Monday, June 04, 2007 5:08 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Clinical use of Co-path Buttoning for us is what the rest of the world calls grossing. Laurie -----Original Message----- From: Victor Tobias [mailto:victor@pathology.washington.edu] Sent: Monday, June 04, 2007 9:35 AM To: Laurie Popp Subject: Re: [Histonet] Re: Clinical use of Co-path What is buttoning? Laurie Popp wrote: >Hi Mike, > >We use CoPath with barcode readers in our buttoning area at Mayo in >Rochester. The only area in our lab that we are currently using >barcodes is our buttoning/gross description area. > >Laurie Popp >HT Candidate >Mayo Clinic > >-----Original Message----- >From: Mike Pence >Sent: Friday, June 01, 2007 12:33 PM >To: histonet-bounces@lists.utsouthwestern.edu >Subject: Clinical Use of CoPath > > >I was wondering if any of you in the Clinical World are using CoPath as >your computer software and if you are, do you use it with barcode >readers? > >Thanks, >Mike > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------------- -- The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================ == _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From contact <@t> excaliburpathology.com Mon Jun 4 22:04:06 2007 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Mon Jun 4 22:04:09 2007 Subject: [Histonet] paper tea bags for biopsies, a source Re: Biopsy bags Message-ID: <120338.16068.qm@web50103.mail.re2.yahoo.com> Thank you Gayle!!!!!!!!!! The last ones I found were from an herbal company and the health food store. A little large, but they do work. FYI, I found soap molds that make great large specimen molds too! Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 630 N. Broadway Moore, OK 73160 405-759-3953 contact@excaliburpathology.com From wanpto <@t> aol.com Mon Jun 4 22:08:10 2007 From: wanpto <@t> aol.com (wanpto@aol.com) Date: Mon Jun 4 22:08:22 2007 Subject: [Histonet] Fwd: NCSHT Symposia Duke University June 19th 2007 In-Reply-To: <8C96C85BE02D151-1328-2858@webmail-dd17.sysops.aol.com> References: <8C96B8ABB77D86D-1084-25A2@FWM-M06.sysops.aol.com> <8C96C7FF46A6D16-F94-29A1@WEBMAIL-RB02.sysops.aol.com> <8C96C85BE02D151-1328-2858@webmail-dd17.sysops.aol.com> Message-ID: <8C97535E2174A06-39C-2F13@FWM-D34.sysops.aol.com> Please list for the rest of this week.? I appreciate it so much. Warmest Regards Wanda Jones Subject: NCSHT Symposia Duke University June 19th 2007 ?NCSHT Symposia Duke University? Durham, NC ? June 19th 2007 ? ?Registration/ 8:30 AM ?"Histology - The Evolution of Automation" Speaker Carolyn Doan, Leica Microsystems? ?Complimentary Breakfast ?"Optimizing Tissue Processing" Speaker Matthew Hoskin/TBD, Vision BioSystems? ?"Are Your CDs Earning Interests?" Guest Speaker Lamar Jones, Wake Forest University/Baptist Medical Center ?Registration/ 8:30 AM ?"Histology - The Evolution of Automation" Speaker Carolyn Doan, Leica Microsystems? ?Complimentary Breakfast ?"Optimizing Tissue Processing" Speaker Matthew Hoskin/TBD, Vision BioSystems? ?"Are Your CDs Earning Interests?" Guest Speaker Lamar Jones, Wake Forest University/Baptist Medical Center ? Complimentary Luncheon ? "IHC and ISH: Maximizing Quality and Workflow" Speaker Matthew Hoskin/TBD, Vision BioSystems ?AFTERNOON TEA ?"Use of IHC to Expedite Cancer Drug Discovery" Guest Speaker Ryan Williams, MD Anderson Cancer Center ?"Special Immunohistochemical Applications" Guest Speaker Jim Burchette, Duke University Medical Center ?Wine and Cheese Reception ?Slide Review and Open Discussion ? Cost:? NCSHT asks you to donate a new teddy bear (or other stuffed animal) or children?s book to our children?s hospital charity drive as your admission cost for this event.? Registration: Contact Wendy Gibson at:? wendy.gibson@vision-bio.com or (781) 616-1245 by June 8, 2007 Registration: Contact Wendy Gibson at:? wendy.gibson@vision-bio.com or (781) 616-1245 by June 8, 2007 ?Warmest Regards Wanda AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From immrstambo <@t> hotmail.com Tue Jun 5 05:22:10 2007 From: immrstambo <@t> hotmail.com (Chistine Tambasco) Date: Tue Jun 5 05:20:46 2007 Subject: [Histonet] Re: Clinical use of Co-path References: <003701c7a6f4$d7dfade0$9700a8c0@laurie><9AE8AA9E1F644B4AA6C155FB6FD51C630ACB7182@EMAIL.archildrens.org> Message-ID: I can beat that- when I started in 1987 at my hospital they used the "L" molds on a cold metal tray, pouring out of a pot, using a bunsen burner to flame your foreceps. I had only seen that process in a book!.Needless to say I have completely modernized my lab. We've come a long way!! Memories......... Christine Tambasco, HT (ASCP) St. Mary's Hospital @ Amsterdam, New York ----- Original Message ----- From: "Mickie Johnson" To: "'Horn, Hazel V'" ; "'Laurie Popp'" ; Sent: Monday, June 04, 2007 5:47 PM Subject: RE: [Histonet] Re: Clinical use of Co-path > Hi All, > > Buttons refer to the round (and sometimes square) metal cassettes with > snap > lids which were commonly used back in the mono and duo Technicon days. > This > was near the dinosaur era about 30 - 35 or so years ago. Later came > plastic > cassettes and little metal molds made by Tissue Tek. I remember > interviewing > for a job at the Los Angeles County General Hospital in 1973 and they > still > used lead L-molds and had the days supply stacked up about two feet high > beside the embedding area. They used a heated pitcher to fill the molds. I > thought that was archaic in those days because there were peel-a-way molds > and the Tissue Tek embedding center which worked 'better'. Well, so much > for > stimulating that part of my memory. > > Mickie > > Mickie Johnson, B.S., HTL(ASCP) > Mohs Histology Consulting Services, LLC > & Mohs Lab Staffing > 2507 S. Manito Blvd. > Spokane, WA 99203 > 509-954-7134 > Web: www.mohshistotemp.com & www.mohslabstaffing.com > Email: mickie25@netzero.net > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, > Hazel > V > Sent: Monday, June 04, 2007 3:11 PM > To: Laurie Popp; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Clinical use of Co-path > > The first clinical histology job I had one of the pathologist called > cassettes, buttons. > I had never heard that before. > > Hazel Horn > Hazel Horn, HT/HTL (ASCP) > Supervisor of Histology > Arkansas Children's Hospital > 800 Marshall Slot 820 > Little Rock, AR 72202 > > phone 501.364.4240 > fax 501.364.3912 > > visit us on the web at: www.archildrens.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie > Popp > Sent: Monday, June 04, 2007 5:08 PM > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Clinical use of Co-path > > Buttoning for us is what the rest of the world calls grossing. > > Laurie > > -----Original Message----- > From: Victor Tobias [mailto:victor@pathology.washington.edu] > Sent: Monday, June 04, 2007 9:35 AM > To: Laurie Popp > Subject: Re: [Histonet] Re: Clinical use of Co-path > > What is buttoning? > > Laurie Popp wrote: > >>Hi Mike, >> >>We use CoPath with barcode readers in our buttoning area at Mayo in >>Rochester. The only area in our lab that we are currently using >>barcodes is our buttoning/gross description area. >> >>Laurie Popp >>HT Candidate >>Mayo Clinic >> >>-----Original Message----- >>From: Mike Pence >>Sent: Friday, June 01, 2007 12:33 PM >>To: histonet-bounces@lists.utsouthwestern.edu >>Subject: Clinical Use of CoPath >> >> >>I was wondering if any of you in the Clinical World are using CoPath as > >>your computer software and if you are, do you use it with barcode >>readers? >> >>Thanks, >>Mike >> >> >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > > -- > Victor Tobias > Clinical Applications Analyst > University of Washington Medical Center > Dept of Pathology Room BB220 > 1959 NE Pacific > Seattle, WA 98195 > victor@pathology.washington.edu > 206-598-2792 > 206-598-7659 Fax > ================================================= > Privileged, confidential or patient identifiable information may be > contained in this message. This information is meant only for the use of > the > intended recipients. If you are not the intended recipient, or if the > message has been addressed to you in error, do not read, disclose, > reproduce, distribute, disseminate or otherwise use this transmission. > Instead, please notify the sender by reply e-mail, and then destroy all > copies of the message and any attachments. > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ---------------------------------------------------------------------------- > -- > The information contained in this message may be privileged and > confidential > and protected from disclosure. If the reader of this message is not the > intended recipient, or an employee or agent responsible for delivering > this > message to the intended recipient, you are hereby notified that any > dissemination, distribution or copying of this communication is strictly > prohibited. If you have received this communication in error, please > notify > us immediately by replying to the message and deleting it from your > computer. > Thank you. > ============================================================================ > == > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From BMolinari <@t> heart.thi.tmc.edu Tue Jun 5 05:28:32 2007 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Tue Jun 5 05:28:33 2007 Subject: [Histonet] Re: Clinical use of Co-path Message-ID: My boss calls them "crickets". Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Monday, June 04, 2007 5:11 PM To: Laurie Popp; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Clinical use of Co-path The first clinical histology job I had one of the pathologist called cassettes, buttons. I had never heard that before. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Popp Sent: Monday, June 04, 2007 5:08 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Clinical use of Co-path Buttoning for us is what the rest of the world calls grossing. Laurie -----Original Message----- From: Victor Tobias [mailto:victor@pathology.washington.edu] Sent: Monday, June 04, 2007 9:35 AM To: Laurie Popp Subject: Re: [Histonet] Re: Clinical use of Co-path What is buttoning? Laurie Popp wrote: >Hi Mike, > >We use CoPath with barcode readers in our buttoning area at Mayo in >Rochester. The only area in our lab that we are currently using >barcodes is our buttoning/gross description area. > >Laurie Popp >HT Candidate >Mayo Clinic > >-----Original Message----- >From: Mike Pence >Sent: Friday, June 01, 2007 12:33 PM >To: histonet-bounces@lists.utsouthwestern.edu >Subject: Clinical Use of CoPath > > >I was wondering if any of you in the Clinical World are using CoPath as >your computer software and if you are, do you use it with barcode >readers? > >Thanks, >Mike > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ ------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From olek.michalski <@t> nencki.gov.pl Tue Jun 5 06:48:36 2007 From: olek.michalski <@t> nencki.gov.pl (Olek Michalski) Date: Tue Jun 5 06:46:29 2007 Subject: [Histonet] DAB residues removal Message-ID: Dear Histonetters, There are many procedures using DAB for staining. We apply some in our lab, so sometimes there is need for cleaning Coplin jars covered w/ brown residue. I normally do it by putting glassware into NaOH solution containing some detergent over weekend and cleaing w/ brush afterwards. Recently I heard there used to be more efficient protocol for DAB residue removal but I can't find it. I know Dako provides DAB-away kit, but supposedly it would be too much for couple jars per week. Do you know any better way to deal with this problem? Best regards Olek Michalski -- Laboratory of Neurobiology of Development and Evolution Nencki Institute of Experimental Biology ul. Pasteura 3, 02-093 Warszawa, Poland Tel. +48 22 5892268, Fax +48 22 8225342 From rjbuesa <@t> yahoo.com Tue Jun 5 07:19:42 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jun 5 07:19:47 2007 Subject: [Histonet] DAB residues removal In-Reply-To: Message-ID: <762401.19011.qm@web61213.mail.yahoo.com> If it is just to clean glassware, place it in bleach until the stain is removed. If that glassware is to be used again for IHC procedures, you have to be ABSOLUTELY sure that ALL the bleach resideues have been eliminated. Ren? J. Olek Michalski wrote: Dear Histonetters, There are many procedures using DAB for staining. We apply some in our lab, so sometimes there is need for cleaning Coplin jars covered w/ brown residue. I normally do it by putting glassware into NaOH solution containing some detergent over weekend and cleaing w/ brush afterwards. Recently I heard there used to be more efficient protocol for DAB residue removal but I can't find it. I know Dako provides DAB-away kit, but supposedly it would be too much for couple jars per week. Do you know any better way to deal with this problem? Best regards Olek Michalski -- Laboratory of Neurobiology of Development and Evolution Nencki Institute of Experimental Biology ul. Pasteura 3, 02-093 Warszawa, Poland Tel. +48 22 5892268, Fax +48 22 8225342 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get the free Yahoo! toolbar and rest assured with the added security of spyware protection. From derek.papalegis <@t> tufts.edu Tue Jun 5 07:31:56 2007 From: derek.papalegis <@t> tufts.edu (Derek Papalegis) Date: Tue Jun 5 07:32:03 2007 Subject: [Histonet] Question about taking test In-Reply-To: <000901c7a6f4$abf8aa60$0202a8c0@HPPav2> References: <000901c7a6f4$abf8aa60$0202a8c0@HPPav2> Message-ID: <466557BC.1000900@tufts.edu> I have my BS and have been in histology for over 3 years and I finally found the time to take the HT exam. I found the test to be flawed in many aspects and didn't let me accurately demonstrate my knowledge of histology. I knew Carson's book and Bancroft and Gamble's book from front to back and was extremely confident when I walked into the test.. After going through the test, I didn't feel as confident until I saw the tiny word on the screen that everyone wants to see: "PASS". The problem I had with the test is that histology is a very visual discipline. We are expected to be able to view slides and determine what stain was used and if that stain is adequate. The pictures on the exam were frequently blurry, the colors were skewed (the red in trichrome was a pale brown) and seemed to intentionally try to confuse the test taker instead of merely testing the knowledge of the person taking the exam. Over the years I have seen many slides that were not acceptable and could accurately trouble-shoot them but the slides on the exam were not even close to being acceptable. The ASCP set such high standards for stains when there was a practical portion of the exam but they seemed to drop the ball when they set the standards for the pictures that they use for the computer portion. That is my two cents... I feel that someone could know the subject material extremely well but then be tripped up by the seemingly intentionally misleading pictures and questions on the test. -- Derek Papalegis HT (ASCP) Histotechnician Division of Laboratory Animal Medicine Tufts University 136 Harrison Avenue Boston, MA 02111 phone: 617 636-2971 fax: 617 636-8354 Lee & Peggy Wenk wrote: > There are two parts to qualifying to take the HTL exam. First, the BS degree > with 30 hours of biology AND chemistry combined plus a minimum of 1 year's > experience within the last 10 years under pathologist certified by the > American Board of Pathology in Anatomic pathology (or the equivalent of a > PhD. > > Second, the experience must be within the last 10 years, and cover fixation, > microtomy, processing and staining. There are no other specifications. > > So the microtomy CAN be just frozen sections (or just plastics, or just > paraffin, etc.). > > So the staining CAN be just H&E. > > (BTW, This experience criteria is the same for the HT exam.) > > Now, with that said, the exams WILL have questions covering ALL aspects of > histotechnology - fixation, processing, decalcification, microtomy (frozen > and paraffin), all special stains, IHC, tissue ID for all tissues, enzymes > on the HTL, safety, equipment, lab math, etc. > > So anyone who works in just one specialty area (Mohs, derm, GI, etc.), will > be at a disadvantage. They will need a lot of books, atlases, and a lot of > time to learn all that is required. They can pass, but it will take a lot > more work than someone who routinely sees a variety of tissues and does a > variety of special stains. The last two cycles of HTL exams, 51% and 70% of > applicants were able to pass the HTL exam. > > Peggy A. Wenk, HTL(ASCP)SLS > William Beaumont Hospital > Royal Oak, MI 48073 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robyn > Vazquez > Sent: Monday, June 04, 2007 3:25 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Question about taking test > > > Hello, I have a question to ask. I have a co-worker that is interested in > taking the test and has a B.S. We work in a Mohs lab, but not an actual > histology lab where we would do special stains. She has been here almost 4 > years doing mostly Mohs surgeries, very little paraffin processing. As I am > understanding it, is that she would have to have 1 year experience in a > histology lab doing special stains and paraffin cutting and processing? > Since they don't make you do special stains and send them in, then she would > have to prove that she can perform a special stain and that is where the 1 > year experience comes in? > > Thanks > > Robyn > OHSU > > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From b-frederick <@t> northwestern.edu Tue Jun 5 08:04:02 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Tue Jun 5 08:04:16 2007 Subject: [Histonet] Re: Clinical use of Co-path In-Reply-To: Message-ID: <000101c7a772$00971480$d00f7ca5@lurie.northwestern.edu> All, I started out with the technicons (4) and round cassettes and we did not start with the "normal" cassettes until 1990 when we dumped them and got the Shandon XP. Embedding was a paraffin dispensor,forceps warmer (I still love them except for the flame throwing capabilities) and cold plate with those embedding rings. In histo school we even had an ultratechnicon (yuck) Ah those memories. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chistine Tambasco Sent: Tuesday, June 05, 2007 5:22 AM To: mickie25@netzero.net; 'Horn, Hazel V'; 'Laurie Popp'; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Clinical use of Co-path I can beat that- when I started in 1987 at my hospital they used the "L" molds on a cold metal tray, pouring out of a pot, using a bunsen burner to flame your foreceps. I had only seen that process in a book!.Needless to say I have completely modernized my lab. We've come a long way!! Memories......... Christine Tambasco, HT (ASCP) St. Mary's Hospital @ Amsterdam, New York ----- Original Message ----- From: "Mickie Johnson" To: "'Horn, Hazel V'" ; "'Laurie Popp'" ; Sent: Monday, June 04, 2007 5:47 PM Subject: RE: [Histonet] Re: Clinical use of Co-path > Hi All, > > Buttons refer to the round (and sometimes square) metal cassettes with > snap > lids which were commonly used back in the mono and duo Technicon days. > This > was near the dinosaur era about 30 - 35 or so years ago. Later came > plastic > cassettes and little metal molds made by Tissue Tek. I remember > interviewing > for a job at the Los Angeles County General Hospital in 1973 and they > still > used lead L-molds and had the days supply stacked up about two feet high > beside the embedding area. They used a heated pitcher to fill the molds. I > thought that was archaic in those days because there were peel-a-way molds > and the Tissue Tek embedding center which worked 'better'. Well, so much > for > stimulating that part of my memory. > > Mickie > > Mickie Johnson, B.S., HTL(ASCP) > Mohs Histology Consulting Services, LLC > & Mohs Lab Staffing > 2507 S. Manito Blvd. > Spokane, WA 99203 > 509-954-7134 > Web: www.mohshistotemp.com & www.mohslabstaffing.com > Email: mickie25@netzero.net > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, > Hazel > V > Sent: Monday, June 04, 2007 3:11 PM > To: Laurie Popp; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Clinical use of Co-path > > The first clinical histology job I had one of the pathologist called > cassettes, buttons. > I had never heard that before. > > Hazel Horn > Hazel Horn, HT/HTL (ASCP) > Supervisor of Histology > Arkansas Children's Hospital > 800 Marshall Slot 820 > Little Rock, AR 72202 > > phone 501.364.4240 > fax 501.364.3912 > > visit us on the web at: www.archildrens.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie > Popp > Sent: Monday, June 04, 2007 5:08 PM > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Clinical use of Co-path > > Buttoning for us is what the rest of the world calls grossing. > > Laurie > > -----Original Message----- > From: Victor Tobias [mailto:victor@pathology.washington.edu] > Sent: Monday, June 04, 2007 9:35 AM > To: Laurie Popp > Subject: Re: [Histonet] Re: Clinical use of Co-path > > What is buttoning? > > Laurie Popp wrote: > >>Hi Mike, >> >>We use CoPath with barcode readers in our buttoning area at Mayo in >>Rochester. The only area in our lab that we are currently using >>barcodes is our buttoning/gross description area. >> >>Laurie Popp >>HT Candidate >>Mayo Clinic >> >>-----Original Message----- >>From: Mike Pence >>Sent: Friday, June 01, 2007 12:33 PM >>To: histonet-bounces@lists.utsouthwestern.edu >>Subject: Clinical Use of CoPath >> >> >>I was wondering if any of you in the Clinical World are using CoPath as > >>your computer software and if you are, do you use it with barcode >>readers? >> >>Thanks, >>Mike >> >> >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > > -- > Victor Tobias > Clinical Applications Analyst > University of Washington Medical Center > Dept of Pathology Room BB220 > 1959 NE Pacific > Seattle, WA 98195 > victor@pathology.washington.edu > 206-598-2792 > 206-598-7659 Fax > ================================================= > Privileged, confidential or patient identifiable information may be > contained in this message. This information is meant only for the use of > the > intended recipients. If you are not the intended recipient, or if the > message has been addressed to you in error, do not read, disclose, > reproduce, distribute, disseminate or otherwise use this transmission. > Instead, please notify the sender by reply e-mail, and then destroy all > copies of the message and any attachments. > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------------------------------------------------ ---- > -- > The information contained in this message may be privileged and > confidential > and protected from disclosure. If the reader of this message is not the > intended recipient, or an employee or agent responsible for delivering > this > message to the intended recipient, you are hereby notified that any > dissemination, distribution or copying of this communication is strictly > prohibited. If you have received this communication in error, please > notify > us immediately by replying to the message and deleting it from your > computer. > Thank you. > ======================================================================== ==== > == > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Tracy.Bergeron <@t> crl.com Tue Jun 5 08:01:08 2007 From: Tracy.Bergeron <@t> crl.com (Bergeron, Tracy) Date: Tue Jun 5 08:05:28 2007 Subject: [Histonet] Question about taking test References: <000901c7a6f4$abf8aa60$0202a8c0@HPPav2> <466557BC.1000900@tufts.edu> Message-ID: Congratulations Derek, glad to hear you passed the exam. Having taken both the HT and HTL exams I can completely sympathize with Derek on the photomicrographs that are used in the exam. One photo I saw during my HTL exam was supposed to be a blood smear, but it took me several minutes to realize that's what it was. The colors in this and many of the other photo's were extremely off to the point of making the stain and tissue nearly unrecognizable Now that there is no practical exam I believe very strongly that the photomicrographs used on the computer exam for HT and HTL should be of the highest quality possible. Tracy E. Bergeron, BS, HT, HTL (ASCP) Histotechnologist Charles River Laboratories Wilmington, MA 978-658-6000 x 1229 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Derek Papalegis Sent: Tue 6/5/2007 8:31 AM To: lpwenk@sbcglobal.net Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Question about taking test I have my BS and have been in histology for over 3 years and I finally found the time to take the HT exam. I found the test to be flawed in many aspects and didn't let me accurately demonstrate my knowledge of histology. I knew Carson's book and Bancroft and Gamble's book from front to back and was extremely confident when I walked into the test.. After going through the test, I didn't feel as confident until I saw the tiny word on the screen that everyone wants to see: "PASS". The problem I had with the test is that histology is a very visual discipline. We are expected to be able to view slides and determine what stain was used and if that stain is adequate. The pictures on the exam were frequently blurry, the colors were skewed (the red in trichrome was a pale brown) and seemed to intentionally try to confuse the test taker instead of merely testing the knowledge of the person taking the exam. Over the years I have seen many slides that were not acceptable and could accurately trouble-shoot them but the slides on the exam were not even close to being acceptable. The ASCP set such high standards for stains when there was a practical portion of the exam but they seemed to drop the ball when they set the standards for the pictures that they use for the computer portion. That is my two cents... I feel that someone could know the subject material extremely well but then be tripped up by the seemingly intentionally misleading pictures and questions on the test. -- Derek Papalegis HT (ASCP) Histotechnician Division of Laboratory Animal Medicine Tufts University 136 Harrison Avenue Boston, MA 02111 phone: 617 636-2971 fax: 617 636-8354 Lee & Peggy Wenk wrote: > There are two parts to qualifying to take the HTL exam. First, the BS degree > with 30 hours of biology AND chemistry combined plus a minimum of 1 year's > experience within the last 10 years under pathologist certified by the > American Board of Pathology in Anatomic pathology (or the equivalent of a > PhD. > > Second, the experience must be within the last 10 years, and cover fixation, > microtomy, processing and staining. There are no other specifications. > > So the microtomy CAN be just frozen sections (or just plastics, or just > paraffin, etc.). > > So the staining CAN be just H&E. > > (BTW, This experience criteria is the same for the HT exam.) > > Now, with that said, the exams WILL have questions covering ALL aspects of > histotechnology - fixation, processing, decalcification, microtomy (frozen > and paraffin), all special stains, IHC, tissue ID for all tissues, enzymes > on the HTL, safety, equipment, lab math, etc. > > So anyone who works in just one specialty area (Mohs, derm, GI, etc.), will > be at a disadvantage. They will need a lot of books, atlases, and a lot of > time to learn all that is required. They can pass, but it will take a lot > more work than someone who routinely sees a variety of tissues and does a > variety of special stains. The last two cycles of HTL exams, 51% and 70% of > applicants were able to pass the HTL exam. > > Peggy A. Wenk, HTL(ASCP)SLS > William Beaumont Hospital > Royal Oak, MI 48073 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robyn > Vazquez > Sent: Monday, June 04, 2007 3:25 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Question about taking test > > > Hello, I have a question to ask. I have a co-worker that is interested in > taking the test and has a B.S. We work in a Mohs lab, but not an actual > histology lab where we would do special stains. She has been here almost 4 > years doing mostly Mohs surgeries, very little paraffin processing. As I am > understanding it, is that she would have to have 1 year experience in a > histology lab doing special stains and paraffin cutting and processing? > Since they don't make you do special stains and send them in, then she would > have to prove that she can perform a special stain and that is where the 1 > year experience comes in? > > Thanks > > Robyn > OHSU > > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Tue Jun 5 08:12:59 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Jun 5 08:13:12 2007 Subject: [Histonet] Dymo labels Message-ID: <4665291B02000077000062B7@gwmail.harthosp.org> Does anyone use a Dymo label maker for preparing slide labels? Thanks! Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From b-frederick <@t> northwestern.edu Tue Jun 5 08:24:27 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Tue Jun 5 08:24:38 2007 Subject: [Histonet] Question about taking test In-Reply-To: Message-ID: <000201c7a774$da513af0$d00f7ca5@lurie.northwestern.edu> I agree though it has been many years. Mine were just slides - like for a projector) which I had been trained on anyway (slides made from actual slides). My HTL slides were mostly from the color plates of the Dezna Sheehan book so I still know what the stain is on the cover and why the [athologist would want it!!! Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bergeron, Tracy Sent: Tuesday, June 05, 2007 8:01 AM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Question about taking test Congratulations Derek, glad to hear you passed the exam. Having taken both the HT and HTL exams I can completely sympathize with Derek on the photomicrographs that are used in the exam. One photo I saw during my HTL exam was supposed to be a blood smear, but it took me several minutes to realize that's what it was. The colors in this and many of the other photo's were extremely off to the point of making the stain and tissue nearly unrecognizable Now that there is no practical exam I believe very strongly that the photomicrographs used on the computer exam for HT and HTL should be of the highest quality possible. Tracy E. Bergeron, BS, HT, HTL (ASCP) Histotechnologist Charles River Laboratories Wilmington, MA 978-658-6000 x 1229 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Derek Papalegis Sent: Tue 6/5/2007 8:31 AM To: lpwenk@sbcglobal.net Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Question about taking test I have my BS and have been in histology for over 3 years and I finally found the time to take the HT exam. I found the test to be flawed in many aspects and didn't let me accurately demonstrate my knowledge of histology. I knew Carson's book and Bancroft and Gamble's book from front to back and was extremely confident when I walked into the test.. After going through the test, I didn't feel as confident until I saw the tiny word on the screen that everyone wants to see: "PASS". The problem I had with the test is that histology is a very visual discipline. We are expected to be able to view slides and determine what stain was used and if that stain is adequate. The pictures on the exam were frequently blurry, the colors were skewed (the red in trichrome was a pale brown) and seemed to intentionally try to confuse the test taker instead of merely testing the knowledge of the person taking the exam. Over the years I have seen many slides that were not acceptable and could accurately trouble-shoot them but the slides on the exam were not even close to being acceptable. The ASCP set such high standards for stains when there was a practical portion of the exam but they seemed to drop the ball when they set the standards for the pictures that they use for the computer portion. That is my two cents... I feel that someone could know the subject material extremely well but then be tripped up by the seemingly intentionally misleading pictures and questions on the test. -- Derek Papalegis HT (ASCP) Histotechnician Division of Laboratory Animal Medicine Tufts University 136 Harrison Avenue Boston, MA 02111 phone: 617 636-2971 fax: 617 636-8354 Lee & Peggy Wenk wrote: > There are two parts to qualifying to take the HTL exam. First, the BS degree > with 30 hours of biology AND chemistry combined plus a minimum of 1 year's > experience within the last 10 years under pathologist certified by the > American Board of Pathology in Anatomic pathology (or the equivalent of a > PhD. > > Second, the experience must be within the last 10 years, and cover fixation, > microtomy, processing and staining. There are no other specifications. > > So the microtomy CAN be just frozen sections (or just plastics, or just > paraffin, etc.). > > So the staining CAN be just H&E. > > (BTW, This experience criteria is the same for the HT exam.) > > Now, with that said, the exams WILL have questions covering ALL aspects of > histotechnology - fixation, processing, decalcification, microtomy (frozen > and paraffin), all special stains, IHC, tissue ID for all tissues, enzymes > on the HTL, safety, equipment, lab math, etc. > > So anyone who works in just one specialty area (Mohs, derm, GI, etc.), will > be at a disadvantage. They will need a lot of books, atlases, and a lot of > time to learn all that is required. They can pass, but it will take a lot > more work than someone who routinely sees a variety of tissues and does a > variety of special stains. The last two cycles of HTL exams, 51% and 70% of > applicants were able to pass the HTL exam. > > Peggy A. Wenk, HTL(ASCP)SLS > William Beaumont Hospital > Royal Oak, MI 48073 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robyn > Vazquez > Sent: Monday, June 04, 2007 3:25 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Question about taking test > > > Hello, I have a question to ask. I have a co-worker that is interested in > taking the test and has a B.S. We work in a Mohs lab, but not an actual > histology lab where we would do special stains. She has been here almost 4 > years doing mostly Mohs surgeries, very little paraffin processing. As I am > understanding it, is that she would have to have 1 year experience in a > histology lab doing special stains and paraffin cutting and processing? > Since they don't make you do special stains and send them in, then she would > have to prove that she can perform a special stain and that is where the 1 > year experience comes in? > > Thanks > > Robyn > OHSU > > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PIXLEYSK <@t> UCMAIL.UC.EDU Tue Jun 5 08:51:42 2007 From: PIXLEYSK <@t> UCMAIL.UC.EDU (Pixley, Sarah (pixleysk)) Date: Tue Jun 5 08:51:51 2007 Subject: [Histonet] RE: DAB residue In-Reply-To: <200706051310.BOD67209@mprelay1.uc.edu> Message-ID: Dear Olek: As another said, beware of using bleach. We have had inactivation of DAB after storing/using glassware that was treated with bleach. When we are using small amounts of DAB (we are a research lab and sometimes deal with small amounts) and we freeze the extra to use later, we now use only brand new glass scintillation vials. Oddly, we have even found that some plastic scintillation vials cause precipitation of the DAB during/after freezing. For cleaning glassware, we have had a lot of success with a compound called Contrad which we get from Fisher Scientific. This replaces, for us, the older standby, chromerge (chromic acid/sulfuric acid). You soak either glassware or plastic in diluted Contrad for >24 hours and rinse well and it is nice and clean. But be sure and rinse very well. This is a safe compound (relatively speaking-don't drink it!! :-) ). It won't burn your hands and we have used it to clean glassware for use in cell culture. Contrad 70: https://new.fishersci.com/wps/portal/PRODUCTDETAIL?productId=816933&cata logId=29104&pos=2&catCode=RE_SC&fromCat=yes&keepSessionSearchOutPut=true &brCategoryId=null Sincerely, Sarah Pixley Cincinnati From vazquezr <@t> ohsu.edu Tue Jun 5 09:04:31 2007 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Tue Jun 5 09:05:04 2007 Subject: [Histonet] Question about taking test Message-ID: Thanks, I will forward this onto her! >>> "Lee & Peggy Wenk" 6/4/2007 3:06 PM >>> There are two parts to qualifying to take the HTL exam. First, the BS degree with 30 hours of biology AND chemistry combined plus a minimum of 1 year's experience within the last 10 years under pathologist certified by the American Board of Pathology in Anatomic pathology (or the equivalent of a PhD. Second, the experience must be within the last 10 years, and cover fixation, microtomy, processing and staining. There are no other specifications. So the microtomy CAN be just frozen sections (or just plastics, or just paraffin, etc.). So the staining CAN be just H&E. (BTW, This experience criteria is the same for the HT exam.) Now, with that said, the exams WILL have questions covering ALL aspects of histotechnology - fixation, processing, decalcification, microtomy (frozen and paraffin), all special stains, IHC, tissue ID for all tissues, enzymes on the HTL, safety, equipment, lab math, etc. So anyone who works in just one specialty area (Mohs, derm, GI, etc.), will be at a disadvantage. They will need a lot of books, atlases, and a lot of time to learn all that is required. They can pass, but it will take a lot more work than someone who routinely sees a variety of tissues and does a variety of special stains. The last two cycles of HTL exams, 51% and 70% of applicants were able to pass the HTL exam. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robyn Vazquez Sent: Monday, June 04, 2007 3:25 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Question about taking test Hello, I have a question to ask. I have a co-worker that is interested in taking the test and has a B.S. We work in a Mohs lab, but not an actual histology lab where we would do special stains. She has been here almost 4 years doing mostly Mohs surgeries, very little paraffin processing. As I am understanding it, is that she would have to have 1 year experience in a histology lab doing special stains and paraffin cutting and processing? Since they don't make you do special stains and send them in, then she would have to prove that she can perform a special stain and that is where the 1 year experience comes in? Thanks Robyn OHSU _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tim.morken <@t> thermofisher.com Tue Jun 5 10:32:28 2007 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Tue Jun 5 10:32:59 2007 Subject: [Histonet] Question about taking test In-Reply-To: <466557BC.1000900@tufts.edu> References: <000901c7a6f4$abf8aa60$0202a8c0@HPPav2> <466557BC.1000900@tufts.edu> Message-ID: <6BFF6D137DF6BC43B33891BA96E83B1977F53B@PGHCR-EXMB-VS-1.na.fshrnet.com> Derek, glad to know the ASCP is consistent - We had the same problem when I took the test in the late '80's and the pictures were printed in booklets! I fact, I'll bet they're probably the same pictures!! Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Derek Papalegis Sent: Tuesday, June 05, 2007 5:32 AM To: lpwenk@sbcglobal.net Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Question about taking test I have my BS and have been in histology for over 3 years and I finally found the time to take the HT exam. I found the test to be flawed in many aspects and didn't let me accurately demonstrate my knowledge of histology. I knew Carson's book and Bancroft and Gamble's book from front to back and was extremely confident when I walked into the test.. After going through the test, I didn't feel as confident until I saw the tiny word on the screen that everyone wants to see: "PASS". The problem I had with the test is that histology is a very visual discipline. We are expected to be able to view slides and determine what stain was used and if that stain is adequate. The pictures on the exam were frequently blurry, the colors were skewed (the red in trichrome was a pale brown) and seemed to intentionally try to confuse the test taker instead of merely testing the knowledge of the person taking the exam. Over the years I have seen many slides that were not acceptable and could accurately trouble-shoot them but the slides on the exam were not even close to being acceptable. The ASCP set such high standards for stains when there was a practical portion of the exam but they seemed to drop the ball when they set the standards for the pictures that they use for the computer portion. That is my two cents... I feel that someone could know the subject material extremely well but then be tripped up by the seemingly intentionally misleading pictures and questions on the test. -- Derek Papalegis HT (ASCP) Histotechnician Division of Laboratory Animal Medicine Tufts University 136 Harrison Avenue Boston, MA 02111 phone: 617 636-2971 fax: 617 636-8354 Lee & Peggy Wenk wrote: > There are two parts to qualifying to take the HTL exam. First, the BS > degree with 30 hours of biology AND chemistry combined plus a minimum > of 1 year's experience within the last 10 years under pathologist > certified by the American Board of Pathology in Anatomic pathology (or > the equivalent of a PhD. > > Second, the experience must be within the last 10 years, and cover > fixation, microtomy, processing and staining. There are no other specifications. > > So the microtomy CAN be just frozen sections (or just plastics, or > just paraffin, etc.). > > So the staining CAN be just H&E. > > (BTW, This experience criteria is the same for the HT exam.) > > Now, with that said, the exams WILL have questions covering ALL > aspects of histotechnology - fixation, processing, decalcification, > microtomy (frozen and paraffin), all special stains, IHC, tissue ID > for all tissues, enzymes on the HTL, safety, equipment, lab math, etc. > > So anyone who works in just one specialty area (Mohs, derm, GI, etc.), > will be at a disadvantage. They will need a lot of books, atlases, and > a lot of time to learn all that is required. They can pass, but it > will take a lot more work than someone who routinely sees a variety of > tissues and does a variety of special stains. The last two cycles of > HTL exams, 51% and 70% of applicants were able to pass the HTL exam. > > Peggy A. Wenk, HTL(ASCP)SLS > William Beaumont Hospital > Royal Oak, MI 48073 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robyn > Vazquez > Sent: Monday, June 04, 2007 3:25 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Question about taking test > > > Hello, I have a question to ask. I have a co-worker that is > interested in taking the test and has a B.S. We work in a Mohs lab, > but not an actual histology lab where we would do special stains. She > has been here almost 4 years doing mostly Mohs surgeries, very little > paraffin processing. As I am understanding it, is that she would have > to have 1 year experience in a histology lab doing special stains and paraffin cutting and processing? > Since they don't make you do special stains and send them in, then she > would have to prove that she can perform a special stain and that is > where the 1 year experience comes in? > > Thanks > > Robyn > OHSU > > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Tue Jun 5 10:33:46 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Jun 5 10:33:26 2007 Subject: [Histonet] Re: Clinical use of Co-path In-Reply-To: References: Message-ID: <6.0.0.22.1.20070605092933.01b67b30@gemini.msu.montana.edu> A researcher here called them crickets too - much to my amusement. We used L shaped molds up to 10 years ago for embedding large decalcified bone samples. The aluminum tea pot for pouring paraffin resides on my antique collectors item shelf. Gayle Calis At 04:28 AM 6/5/2007, you wrote: >My boss calls them "crickets". > >Betsy Molinari HT (ASCP) >Texas Heart Institute >Cardiovascular Pathology >6770 Bertner Ave. >Houston,TX 77030 >832-355-6524 >832-355-6812 (fax) Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 From gcallis <@t> montana.edu Tue Jun 5 10:40:32 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Jun 5 10:40:11 2007 Subject: [Histonet] Question about taking test In-Reply-To: <466557BC.1000900@tufts.edu> References: <000901c7a6f4$abf8aa60$0202a8c0@HPPav2> <466557BC.1000900@tufts.edu> Message-ID: <6.0.0.22.1.20070605093458.01b3a9a0@gemini.msu.montana.edu> Derek, There have been many histotechnicians involved with setting up testing for years who have been trying to correct the problem of poor photos, but ASCP is slow to change, so it seems. It is the testing agency who drops the ball here. I suggest you send this email message in the form of a hard copy letter to the testing agency (CAP or ASCP), express your unhappiness about poor photographs. Maybe that will speed things along to aid others when taking the HT or HTL exams. At 06:31 AM 6/5/2007, you wrote: >I have my BS and have been in histology for over 3 years and I finally >found the time to take the HT exam. I found the test to be flawed in many >aspects and didn't let me accurately demonstrate my knowledge of >histology. I knew Carson's book and Bancroft and Gamble's book from front >to back and was extremely confident when I walked into the test.. After >going through the test, I didn't feel as confident until I saw the tiny >word on the screen that everyone wants to see: "PASS". The problem I had >with the test is that histology is a very visual discipline. We are >expected to be able to view slides and determine what stain was used and >if that stain is adequate. The pictures on the exam were frequently >blurry, the colors were skewed (the red in trichrome was a pale brown) and >seemed to intentionally try to confuse the test taker instead of merely >testing the knowledge of the person taking the exam. Over the years I have >seen many slides that were not acceptable and could accurately >trouble-shoot them but the slides on the exam were not even close to being >acceptable. The ASCP set such high standards for stains when there was a >practical portion of the exam but they seemed to drop the ball when they >set the standards for the pictures that they use for the computer portion. > >That is my two cents... I feel that someone could know the subject >material extremely well but then be tripped up by the seemingly >intentionally misleading pictures and questions on the test. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From rsrichmond <@t> aol.com Tue Jun 5 10:44:01 2007 From: rsrichmond <@t> aol.com (rsrichmond@aol.com) Date: Tue Jun 5 10:44:13 2007 Subject: [Histonet] safranin for marking small specimens In-Reply-To: <200706050906.11346655fe7164@rly-mc03.mail.aol.com> References: <200706050906.11346655fe7164@rly-mc03.mail.aol.com> Message-ID: <8C9759F79019124-1FC8-46D1@mblk-r28.sysops.aol.com> Marking small biopsy specimens so that the embedder can find them after processing - this question has been raised several times on Histonet, particularly since a favorite marking dye, Mercurochrome, is no longer available. Recently in my travels I've found a service that uses safranin for this purpose. The tissue is put on lens paper or on a blue biopsy foam pad, and the dye is dabbed onto the tissue with the tip of a wooden applicator stick. Tissue is easily visible to the embedder, and is visible in the finished paraffin block. The technique is particularly valuable on this pathology service, where both the pathologists (including me!) and the histotechnologist have elderly eyes, illumination is insufficient, and magnification is not available. We're not talking about marking margins (india ink and colored inks). The safranin is not visible in the slides. The safranin solution is the one used as the Gram stain counterstain by the microbiology laboratory, so that nothing new needs to be ordered. Specifically, it's safranin (Colour Index 50240, not saffron) 0.6% w/v, in 20% reagent alcohol. Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From buri <@t> seattleskincancer.com Mon Jun 4 10:54:45 2007 From: buri <@t> seattleskincancer.com (Beth Uri) Date: Tue Jun 5 11:11:39 2007 Subject: [Histonet] Mohs position Seattle Message-ID: <006d01c7a6c0$b1d9c880$2500000a@corp.birkby.com> Hi, I have a temporary (3 months guaranteed), part-time position to cut Mohs sections in Seattle. Willing to train. If interested please email me at buri@seattleskincancer.com Thanks, Beth From mber <@t> vt.edu Tue Jun 5 11:22:13 2007 From: mber <@t> vt.edu (mber@vt.edu) Date: Tue Jun 5 11:22:19 2007 Subject: [Histonet] Frozen sectioning of mouse mammary tissue Message-ID: <1181060533.46658db55dc03@webmail.vt.edu> Hi all, I have a grad student who needs help with frozen sectioning of mouse mammary tissue. He has not been able to cut any sections because it is fatty tissue. I haven't been able to watch him cut yet; I will be doing that tomorrow. In the meantime, if someone has suggestions and also a protocol, that would be very helpful. Thanks again. Meg Berger Histologist Animal and Poultry Sciences VA Tech From derek.papalegis <@t> tufts.edu Tue Jun 5 11:30:15 2007 From: derek.papalegis <@t> tufts.edu (Derek Papalegis) Date: Tue Jun 5 11:30:19 2007 Subject: [Histonet] Frozen sectioning of mouse mammary tissue In-Reply-To: <1181060533.46658db55dc03@webmail.vt.edu> References: <1181060533.46658db55dc03@webmail.vt.edu> Message-ID: <46658F97.8010601@tufts.edu> Meg, Drop the temp down to -30. That usually helps. Spray the blade and the anti-roll plate with cryo spray as well. -Derek mber@vt.edu wrote: > Hi all, > I have a grad student who needs help with frozen sectioning of mouse mammary > tissue. He has not been able to cut any sections because it is fatty tissue. I > haven't been able to watch him cut yet; I will be doing that tomorrow. In the > meantime, if someone has suggestions and also a protocol, that would be very > helpful. Thanks again. > Meg Berger > Histologist > Animal and Poultry Sciences > VA Tech > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Derek Papalegis HT (ASCP) Histotechnician Division of Laboratory Animal Medicine Tufts University 136 Harrison Avenue Boston, MA 02111 phone: 617 636-2971 fax: 617 636-8354 From b-frederick <@t> northwestern.edu Tue Jun 5 11:44:19 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Tue Jun 5 11:44:58 2007 Subject: [Histonet] safranin for marking small specimens In-Reply-To: <8C9759F79019124-1FC8-46D1@mblk-r28.sysops.aol.com> Message-ID: <000701c7a790$c6856a70$d00f7ca5@lurie.northwestern.edu> We put eosin in the 95% or in the absolute. on the microwave Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of rsrichmond@aol.com Sent: Tuesday, June 05, 2007 10:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] safranin for marking small specimens Marking small biopsy specimens so that the embedder can find them after processing - this question has been raised several times on Histonet, particularly since a favorite marking dye, Mercurochrome, is no longer available. Recently in my travels I've found a service that uses safranin for this purpose. The tissue is put on lens paper or on a blue biopsy foam pad, and the dye is dabbed onto the tissue with the tip of a wooden applicator stick. Tissue is easily visible to the embedder, and is visible in the finished paraffin block. The technique is particularly valuable on this pathology service, where both the pathologists (including me!) and the histotechnologist have elderly eyes, illumination is insufficient, and magnification is not available. We're not talking about marking margins (india ink and colored inks). The safranin is not visible in the slides. The safranin solution is the one used as the Gram stain counterstain by the microbiology laboratory, so that nothing new needs to be ordered. Specifically, it's safranin (Colour Index 50240, not saffron) 0.6% w/v, in 20% reagent alcohol. Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Tue Jun 5 11:57:58 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Tue Jun 5 11:58:05 2007 Subject: [Histonet] Replacement stropping wheels for H/I 76 Hacker Knife Sharpener? Message-ID: <4EBFF65383B74D49995298C4976D1D5E273C97@LSRIEXCH1.lsmaster.lifespan.org> Anyone know where I can purchase them? From mpence <@t> grhs.net Tue Jun 5 12:23:36 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Jun 5 12:23:41 2007 Subject: [Histonet] safranin for marking small specimens In-Reply-To: <8C9759F79019124-1FC8-46D1@mblk-r28.sysops.aol.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C615@IS-E2K3.grhs.net> We put about 10 mL of our staining eosin in the first ETOH and this is enough to give just some color to the tissue no matter how small for those "aging eyes" Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of rsrichmond@aol.com Sent: Tuesday, June 05, 2007 10:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] safranin for marking small specimens Marking small biopsy specimens so that the embedder can find them after processing - this question has been raised several times on Histonet, particularly since a favorite marking dye, Mercurochrome, is no longer available. Recently in my travels I've found a service that uses safranin for this purpose. The tissue is put on lens paper or on a blue biopsy foam pad, and the dye is dabbed onto the tissue with the tip of a wooden applicator stick. Tissue is easily visible to the embedder, and is visible in the finished paraffin block. The technique is particularly valuable on this pathology service, where both the pathologists (including me!) and the histotechnologist have elderly eyes, illumination is insufficient, and magnification is not available. We're not talking about marking margins (india ink and colored inks). The safranin is not visible in the slides. The safranin solution is the one used as the Gram stain counterstain by the microbiology laboratory, so that nothing new needs to be ordered. Specifically, it's safranin (Colour Index 50240, not saffron) 0.6% w/v, in 20% reagent alcohol. Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Wanda.Smith <@t> HCAhealthcare.com Tue Jun 5 12:23:58 2007 From: Wanda.Smith <@t> HCAhealthcare.com (Smith Wanda) Date: Tue Jun 5 12:24:21 2007 Subject: [Histonet] Twin Placenta's Message-ID: <817C2761C5A1394180709AEEDB775B7E029C05AB@NASEV03.hca.corpad.net> Good Afternoon, My question is: How do you bill for twin placentas that the OB has designated the cords as Baby A and Baby B? Should that be two 88307's for the designation of different "specimens"? Thanks, Wanda Wanda G. Smith, HTL(ASCP)HT Trident Medical Center Pathology Supervisor ph: 843-847-4586 fx: 843-847-4296 email: wanda.smith@hcahealthcare.com From mpence <@t> grhs.net Tue Jun 5 12:26:48 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Jun 5 12:27:00 2007 Subject: [Histonet] safranin for marking small specimens In-Reply-To: <661949901A768E4F9CC16D8AF8F2838CA1C615@IS-E2K3.grhs.net> Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C616@IS-E2K3.grhs.net> Of our tissue processor that is. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Tuesday, June 05, 2007 12:24 PM To: rsrichmond@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] safranin for marking small specimens We put about 10 mL of our staining eosin in the first ETOH and this is enough to give just some color to the tissue no matter how small for those "aging eyes" Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of rsrichmond@aol.com Sent: Tuesday, June 05, 2007 10:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] safranin for marking small specimens Marking small biopsy specimens so that the embedder can find them after processing - this question has been raised several times on Histonet, particularly since a favorite marking dye, Mercurochrome, is no longer available. Recently in my travels I've found a service that uses safranin for this purpose. The tissue is put on lens paper or on a blue biopsy foam pad, and the dye is dabbed onto the tissue with the tip of a wooden applicator stick. Tissue is easily visible to the embedder, and is visible in the finished paraffin block. The technique is particularly valuable on this pathology service, where both the pathologists (including me!) and the histotechnologist have elderly eyes, illumination is insufficient, and magnification is not available. We're not talking about marking margins (india ink and colored inks). The safranin is not visible in the slides. The safranin solution is the one used as the Gram stain counterstain by the microbiology laboratory, so that nothing new needs to be ordered. Specifically, it's safranin (Colour Index 50240, not saffron) 0.6% w/v, in 20% reagent alcohol. Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Tue Jun 5 12:27:48 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Jun 5 12:27:31 2007 Subject: [Histonet] Replacement stropping wheels for H/I 76 Hacker Knife Sharpener? In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E273C97@LSRIEXCH1.lsmaster. lifespan.org> References: <4EBFF65383B74D49995298C4976D1D5E273C97@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <6.0.0.22.1.20070605112538.01b33648@gemini.msu.montana.edu> www.hackerintruments.com or maybe IMEB At 10:57 AM 6/5/2007, you wrote: >Anyone know where I can purchase them? >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From AWempren <@t> skcc.org Tue Jun 5 12:29:32 2007 From: AWempren <@t> skcc.org (Alexina Wempren) Date: Tue Jun 5 12:29:36 2007 Subject: [Histonet] Perfusing Rat Lung Message-ID: Does anyone have a good protocol for perfusing rat lung? We have been flushing with ringers, then 10% NBF, then ringers and ending with 30% sucrose overnight but the morphology is not good. We add 0.8cc of air so the airways and vessels are not flat but it's still not great. We used to use paraformaldehyde but my supervisor did not like it. Any help would be greatly appreciated. Thanks, alexina From mpence <@t> grhs.net Tue Jun 5 12:31:00 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Jun 5 12:31:10 2007 Subject: [Histonet] Twin Placenta's In-Reply-To: <817C2761C5A1394180709AEEDB775B7E029C05AB@NASEV03.hca.corpad.net> Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C617@IS-E2K3.grhs.net> If they designate "a" and "b" then we are charging 88307 x 2 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith Wanda Sent: Tuesday, June 05, 2007 12:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Twin Placenta's Good Afternoon, My question is: How do you bill for twin placentas that the OB has designated the cords as Baby A and Baby B? Should that be two 88307's for the designation of different "specimens"? Thanks, Wanda Wanda G. Smith, HTL(ASCP)HT Trident Medical Center Pathology Supervisor ph: 843-847-4586 fx: 843-847-4296 email: wanda.smith@hcahealthcare.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gvdobbin <@t> ihis.org Tue Jun 5 12:45:12 2007 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Tue Jun 5 12:47:24 2007 Subject: [Histonet] Perfusing Rat Lung Message-ID: I have 2 references for you: Microscopic changes induced by the intratracheal inoculation of amniotic fluid and meconium in the lung of neonatal rats. J. Martinez-Burnes, et al. Histology and Histopathology (2002) 17:1067-76. and Ultrastructural changes in the lungs of neonatal rats intratracheally inoculated with meconium. J. Martinez et al. Histology and Histopathology (2003) 18:1081-94. If you prefer, I can fax you the Materials and Methods section(s) that describe the perfusion protocol that produced amazing results! Cheers! Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 >>> "Alexina Wempren" 6/5/2007 2:29 PM >>> Does anyone have a good protocol for perfusing rat lung? We have been flushing with ringers, then 10% NBF, then ringers and ending with 30% sucrose overnight but the morphology is not good. We add 0.8cc of air so the airways and vessels are not flat but it's still not great. We used to use paraformaldehyde but my supervisor did not like it. Any help would be greatly appreciated. Thanks, alexina _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. Déclaration de confidentialité Le présent message (y compris les annexes) peut contenir des renseignements confidentiels ayant pour objet une personne ou un organisme particulier. Si vous avez reçu la présente communication par erreur, veuillez en informer l'expéditeur immédiatement. Si vous n'êtes pas le destinataire prévu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer complètement de votre système informatique. From pruegg <@t> ihctech.net Tue Jun 5 13:01:52 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Jun 5 13:02:05 2007 Subject: [Histonet] solvent recycler Message-ID: <001701c7a79b$9e3e4900$6501a8c0@Patsy> I am in the market for an alcohol and xylene recycler for a small volume generator lab. I have seen some talk about CGB units. Please if you have experiences to share on these send them to me. Vendors are invited to reply, but I want an out right price for a small volume unit, I do not want to sign up for a monthly service. Are these ever sold as reconditioned or used units? Thank you, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC 12635 Montview Blvd. Ste.215 Aurora, Colorado 80045 Phone: 720-859-4060 Fax: 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org From mcauliff <@t> umdnj.edu Tue Jun 5 13:19:54 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Jun 5 13:20:32 2007 Subject: [Histonet] Perfusing Rat Lung In-Reply-To: References: Message-ID: <4665A94A.5040108@umdnj.edu> Hi alexina: First off, why replace the fixative with Ringer's? Remember formalin works slowly so removing it will work against good morphology. What about the morphology is not to your liking? If you instill fixative down the trachea with the lungs in the animal and the chest cavity closed you might get better results. Geoff Alexina Wempren wrote: > Does anyone have a good protocol for perfusing rat lung? We have been > flushing with ringers, then 10% NBF, then ringers and ending with 30% > sucrose overnight but the morphology is not good. We add 0.8cc of air so > the airways and vessels are not flat but it's still not great. We used > to use paraformaldehyde but my supervisor did not like it. Any help > would be greatly appreciated. > > Thanks, alexina > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From HACKERLAB <@t> aol.com Tue Jun 5 13:45:42 2007 From: HACKERLAB <@t> aol.com (HACKERLAB@aol.com) Date: Tue Jun 5 13:46:02 2007 Subject: [Histonet] Replacement stropping wheels for H/I 76 Hacker Knife Sharpener? Message-ID: In response to your question: You can purchase the honing & stropping wheels from Hacker Instruments & Industries Inc. 1-800-4-HACKER or 803-712-6100 - _hackerlab@aol.com_ (mailto:hackerlab@aol.com) We look forward to assisting you. With best regards, Carla Young Sales Administrator HACKER Instruments & Industries Inc 1132 Kincaid Bridge Road P.O. Box 1176 Winnsboro, SC 29180 USA Tel: ++(803) 712-6100 Fax: ++(803) 712-6116 e-mail: hackerlab@aol.com _www.hackerinstruments.com_ (http://www.hackerinstruments.com) ************************************** See what's free at http://www.aol.com. From Charles.Embrey <@t> carle.com Tue Jun 5 14:25:02 2007 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Tue Jun 5 14:25:10 2007 Subject: [Histonet] solvent recycler In-Reply-To: <001701c7a79b$9e3e4900$6501a8c0@Patsy> Message-ID: <44780C571F28624DBB446DE55C4D733A1FE4EE@EXCHANGEBE1.carle.com> Hi Patsy. CBG has small units that do 2.5 gal and sit on your countertop. I am sure they can set you up with a demo and then you can see first hand if it will meet your needs.. Charles Embrey PA(ASCP) Histology Manager Carle Clinic Illinois -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Tuesday, June 05, 2007 1:02 PM To: 'Histonet' Subject: [Histonet] solvent recycler I am in the market for an alcohol and xylene recycler for a small volume generator lab. I have seen some talk about CGB units. Please if you have experiences to share on these send them to me. Vendors are invited to reply, but I want an out right price for a small volume unit, I do not want to sign up for a monthly service. Are these ever sold as reconditioned or used units? Thank you, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC 12635 Montview Blvd. Ste.215 Aurora, Colorado 80045 Phone: 720-859-4060 Fax: 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dsprshospital <@t> yahoo.com Tue Jun 5 14:28:42 2007 From: dsprshospital <@t> yahoo.com (Des Peres Hospital) Date: Tue Jun 5 14:28:50 2007 Subject: [Histonet] OR Specimens Sent to Lab Message-ID: <319566.56554.qm@web63711.mail.re1.yahoo.com> Hello All: I am gathering data about what types of samples removed from surgery are NOT sent to the lab. All feedback is appreciated. Susan --------------------------------- Boardwalk for $500? In 2007? Ha! Play Monopoly Here and Now (it's updated for today's economy) at Yahoo! Games. From bakerj <@t> umich.edu Tue Jun 5 14:29:17 2007 From: bakerj <@t> umich.edu (John Baker) Date: Tue Jun 5 14:36:00 2007 Subject: [Histonet] Paraffin sectioning rat brain Message-ID: <312a748ffb8ccf8aa2c0dbb0202eaa42@umich.edu> Hello All, Can someone give me any insight into the procedure for getting good 7 micron paraffin sections? I work with bone usually but am doing work for a Neurosurgery group. I process my slices of rat and gerbil brain for 8 hours and then embed in Paraplast Plus. I ice my blocks before taking ribbons and then float the sections on an alcohol 20% EtOH pre-float bath then transfer the sections to a 50 degree C waterbath. I use Superfrost Plus slides and heat them over night on a 45 degree C warming table. I do a Hematoxylin -Gills 3 and alcoholic eosin stain. The doctor is trying to detect small infarctions and areas showing ischemic neuronal changes - eosinophilic cytoplasm and nuclear changes such as pyknosis, karyolysis and karyorrhexis. Cellular detail is good but I am getting fine wrinkles in the tissue. Any suggestions in my method appreciated. Thanks in advance. John John A. Baker The University of Michigan Orthopaedic Research Laboratories Histology Unit 109 Zina Pitcher Place, 2218 BSRB Ann Arbor, MI 48109-2200 734-936-1635 From stamptrain <@t> yahoo.com Tue Jun 5 14:44:52 2007 From: stamptrain <@t> yahoo.com (Roger Moretz) Date: Tue Jun 5 14:44:59 2007 Subject: [Histonet] Paraffin sectioning rat brain In-Reply-To: <312a748ffb8ccf8aa2c0dbb0202eaa42@umich.edu> Message-ID: <408646.86746.qm@web55808.mail.re3.yahoo.com> Wow! My procedure seems trivial, but here goes: Ice the blocks by placing in wet ice (enough water to wet the tissue) for about 10-15min; collect the ribbon and float on a 46C water bath and pick up on either Plus or uncoated slides (I prefer uncoated for brain). Airdry overnight, 60C oven for 10min (it's part of the cycle on the Leica stainer) & stain with hematoxylin & eosin. I may occasionally get some wrinkles but that is usually due to the use of the coated slides. One comment--leaving the brains on ice too long can cause the tissue to expand too fast on the waterbath. Roger Moretz, Ph.D. Dept of Toxicology Boehringer Ingelheim Pharmaceuticals Ridgefield, CT\ --- John Baker wrote: > Hello All, Can someone give me any insight into the > procedure for > getting good 7 micron paraffin sections? I work > with bone usually but > am doing work for a Neurosurgery group. I process > my slices of rat and > gerbil brain for 8 hours and then embed in Paraplast > Plus. I ice my > blocks before taking ribbons and then float the > sections on an alcohol > 20% EtOH pre-float bath then transfer the sections > to a 50 degree C > waterbath. I use Superfrost Plus slides and heat > them over night on a > 45 degree C warming table. I do a Hematoxylin > -Gills 3 and alcoholic > eosin stain. The doctor is trying to detect small > infarctions and > areas showing ischemic neuronal changes - > eosinophilic cytoplasm and > nuclear changes such as pyknosis, karyolysis and > karyorrhexis. > Cellular detail is good but I am getting fine > wrinkles in the tissue. > Any suggestions in my method appreciated. Thanks in > advance. John > > John A. Baker > The University of Michigan > Orthopaedic Research Laboratories > Histology Unit > 109 Zina Pitcher Place, 2218 BSRB > Ann Arbor, MI 48109-2200 > 734-936-1635 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ____________________________________________________________________________________ Don't pick lemons. See all the new 2007 cars at Yahoo! Autos. http://autos.yahoo.com/new_cars.html From CIngles <@t> uwhealth.org Tue Jun 5 16:17:31 2007 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Tue Jun 5 16:20:02 2007 Subject: [Histonet] safranin for marking small specimens References: <200706050906.11346655fe7164@rly-mc03.mail.aol.com> <8C9759F79019124-1FC8-46D1@mblk-r28.sysops.aol.com> Message-ID: <08A0A863637F1349BBFD83A96B27A50A12004B@uwhis-xchng3.uwhis.hosp.wisc.edu> We use a small drop of Hematoxylin on the tissue when grossing. It shows up great, and you're going to stain with Heme later anyway, so it doesn't interfere with subsequent staining. Claire Ingles UW Hospital Madison WI ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of rsrichmond@aol.com Sent: Tue 6/5/2007 10:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] safranin for marking small specimens Marking small biopsy specimens so that the embedder can find them after processing - this question has been raised several times on Histonet, particularly since a favorite marking dye, Mercurochrome, is no longer available. Recently in my travels I've found a service that uses safranin for this purpose. The tissue is put on lens paper or on a blue biopsy foam pad, and the dye is dabbed onto the tissue with the tip of a wooden applicator stick. Tissue is easily visible to the embedder, and is visible in the finished paraffin block. The technique is particularly valuable on this pathology service, where both the pathologists (including me!) and the histotechnologist have elderly eyes, illumination is insufficient, and magnification is not available. We're not talking about marking margins (india ink and colored inks). The safranin is not visible in the slides. The safranin solution is the one used as the Gram stain counterstain by the microbiology laboratory, so that nothing new needs to be ordered. Specifically, it's safranin (Colour Index 50240, not saffron) 0.6% w/v, in 20% reagent alcohol. Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Tue Jun 5 16:25:13 2007 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Tue Jun 5 16:27:43 2007 Subject: [Histonet] Frozen sectioning of mouse mammary tissue References: <1181060533.46658db55dc03@webmail.vt.edu> <46658F97.8010601@tufts.edu> Message-ID: <08A0A863637F1349BBFD83A96B27A50A12004C@uwhis-xchng3.uwhis.hosp.wisc.edu> Liquid Nitrogen helps too! The spray kind, not emersion. The OCT gets too cold and cracks before the tissue gets cold enough. Claire Ingles UW Hospital Madison WI ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Derek Papalegis Sent: Tue 6/5/2007 11:30 AM To: mber@vt.edu Cc: Histonet Subject: Re: [Histonet] Frozen sectioning of mouse mammary tissue Meg, Drop the temp down to -30. That usually helps. Spray the blade and the anti-roll plate with cryo spray as well. -Derek From Charlene.Henry <@t> STJUDE.ORG Tue Jun 5 16:30:03 2007 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Tue Jun 5 16:30:11 2007 Subject: [Histonet] Unsubscribe - Going on Vacation Message-ID: <5CB39BCA5724F349BCB748675C6CA1A214543487@SJMEMXMB02.stjude.sjcrh.local> Charlene From jnocito <@t> satx.rr.com Tue Jun 5 17:25:46 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Jun 5 17:25:56 2007 Subject: [Histonet] Twin Placenta's References: <817C2761C5A1394180709AEEDB775B7E029C05AB@NASEV03.hca.corpad.net> Message-ID: <008701c7a7c0$770a9490$d49eae18@yourxhtr8hvc4p> Wanda, yep 88307 x 2. Just when surgeons designate left an right tonsils in the same container. JTT ----- Original Message ----- From: "Smith Wanda" To: Sent: Tuesday, June 05, 2007 12:23 PM Subject: [Histonet] Twin Placenta's Good Afternoon, My question is: How do you bill for twin placentas that the OB has designated the cords as Baby A and Baby B? Should that be two 88307's for the designation of different "specimens"? Thanks, Wanda Wanda G. Smith, HTL(ASCP)HT Trident Medical Center Pathology Supervisor ph: 843-847-4586 fx: 843-847-4296 email: wanda.smith@hcahealthcare.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Tue Jun 5 17:36:38 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Jun 5 17:36:50 2007 Subject: [Histonet] Question about taking test References: <000901c7a6f4$abf8aa60$0202a8c0@HPPav2> <466557BC.1000900@tufts.edu> Message-ID: <00cb01c7a7c1$fbec90e0$d49eae18@yourxhtr8hvc4p> I agree with Derek on this. Although when I took my HT exam, we used parchment paper and coal, but when I took my PA exam last year, I was livid. All the photomics where gray and pale blue. I almost blew several questions because of the poor quality. I don't think it's so much ASCP as it is the testing center computers may be off color. When I took my exam, others were taking the GRE, GED, and other tests, but our exams are probably the only one that needs critical resolution and color. My 4 cents worth. Derek, congratulations on passing. JTT ----- Original Message ----- From: "Derek Papalegis" To: Cc: Sent: Tuesday, June 05, 2007 7:31 AM Subject: Re: [Histonet] Question about taking test >I have my BS and have been in histology for over 3 years and I finally >found the time to take the HT exam. I found the test to be flawed in many >aspects and didn't let me accurately demonstrate my knowledge of histology. >I knew Carson's book and Bancroft and Gamble's book from front to back and >was extremely confident when I walked into the test.. After going through >the test, I didn't feel as confident until I saw the tiny word on the >screen that everyone wants to see: "PASS". The problem I had with the test >is that histology is a very visual discipline. We are expected to be able >to view slides and determine what stain was used and if that stain is >adequate. The pictures on the exam were frequently blurry, the colors were >skewed (the red in trichrome was a pale brown) and seemed to intentionally >try to confuse the test taker instead of merely testing the knowledge of >the person taking the exam. Over the years I have seen many slides that >were not acceptable and could accurately trouble-shoot them but the slides >on the exam were not even close to being acceptable. The ASCP set such high >standards for stains when there was a practical portion of the exam but >they seemed to drop the ball when they set the standards for the pictures >that they use for the computer portion. > > That is my two cents... I feel that someone could know the subject > material extremely well but then be tripped up by the seemingly > intentionally misleading pictures and questions on the test. > > -- > Derek Papalegis HT (ASCP) > Histotechnician > Division of Laboratory Animal Medicine > Tufts University 136 Harrison Avenue > Boston, MA 02111 > phone: 617 636-2971 > fax: 617 636-8354 > > > > Lee & Peggy Wenk wrote: >> There are two parts to qualifying to take the HTL exam. First, the BS >> degree >> with 30 hours of biology AND chemistry combined plus a minimum of 1 >> year's >> experience within the last 10 years under pathologist certified by the >> American Board of Pathology in Anatomic pathology (or the equivalent of a >> PhD. >> >> Second, the experience must be within the last 10 years, and cover >> fixation, >> microtomy, processing and staining. There are no other specifications. >> >> So the microtomy CAN be just frozen sections (or just plastics, or just >> paraffin, etc.). >> >> So the staining CAN be just H&E. >> >> (BTW, This experience criteria is the same for the HT exam.) >> >> Now, with that said, the exams WILL have questions covering ALL aspects >> of >> histotechnology - fixation, processing, decalcification, microtomy >> (frozen >> and paraffin), all special stains, IHC, tissue ID for all tissues, >> enzymes >> on the HTL, safety, equipment, lab math, etc. >> >> So anyone who works in just one specialty area (Mohs, derm, GI, etc.), >> will >> be at a disadvantage. They will need a lot of books, atlases, and a lot >> of >> time to learn all that is required. They can pass, but it will take a lot >> more work than someone who routinely sees a variety of tissues and does a >> variety of special stains. The last two cycles of HTL exams, 51% and 70% >> of >> applicants were able to pass the HTL exam. >> >> Peggy A. Wenk, HTL(ASCP)SLS >> William Beaumont Hospital >> Royal Oak, MI 48073 >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robyn >> Vazquez >> Sent: Monday, June 04, 2007 3:25 PM >> To: Histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Question about taking test >> >> Hello, I have a question to ask. I have a co-worker that is interested >> in >> taking the test and has a B.S. We work in a Mohs lab, but not an actual >> histology lab where we would do special stains. She has been here almost >> 4 >> years doing mostly Mohs surgeries, very little paraffin processing. As I >> am >> understanding it, is that she would have to have 1 year experience in a >> histology lab doing special stains and paraffin cutting and processing? >> Since they don't make you do special stains and send them in, then she >> would >> have to prove that she can perform a special stain and that is where the >> 1 >> year experience comes in? >> Thanks >> Robyn >> OHSU >> >> >> >> >> >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From immrstambo <@t> hotmail.com Tue Jun 5 18:52:11 2007 From: immrstambo <@t> hotmail.com (Christine Tambasco) Date: Tue Jun 5 18:52:18 2007 Subject: [Histonet] Perfusing Rat Lung In-Reply-To: Message-ID: It's been a few years since I did research, but we used to just use 10% NBF and we never had any complaints...you might want to give it a try. CHristine Tambasco, HT (ASCP) St. Marys Hospital @ Amsterdam, New York ______________________________________________________________ From: "Alexina Wempren" To: Subject: [Histonet] Perfusing Rat Lung Date: Tue, 5 Jun 2007 10:29:32 -0700 >Does anyone have a good protocol for perfusing rat lung? We have been >flushing with ringers, then 10% NBF, then ringers and ending with 30% >sucrose overnight but the morphology is not good. We add 0.8cc of air so >the airways and vessels are not flat but it's still not great. We used >to use paraformaldehyde but my supervisor did not like it. Any help >would be greatly appreciated. > > Thanks, alexina > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ [1]Make every IM count. Download Messenger and join the im Initiative now. Its free. References 1. http://g.msn.com/8HMBENUS/2755??PS=47575 From KatlebMF <@t> ah.org Wed Jun 6 04:28:29 2007 From: KatlebMF <@t> ah.org (Maria Katleba) Date: Wed Jun 6 04:29:25 2007 Subject: [Histonet] at new job Message-ID: <46661BCD.905C.0090.0@ah.org> Hi All, Just a reminder, if you need to contact me I will no longer be here at St Helena Hospital. I am at Queen of the Valley Medical Center , Pathology Dept Mgr my email MariaKatleba@aol.com Keep in touch Maria Katleba, HTL MS From MAUGER <@t> email.chop.edu Wed Jun 6 07:46:27 2007 From: MAUGER <@t> email.chop.edu (Joanne Mauger) Date: Wed Jun 6 07:47:03 2007 Subject: [Histonet] Paraffin sectioning rat brain Message-ID: John, Try drying your slides vertically instead of flat-overnight at room temp or in an oven below 50 C. That shloud help the water drain off better, and the tissue should be less wrinkled. Our brains cut better at room temp than cold. Also allow them to spread out nicely on the warm water bath before you pick them up. Jo From gcallis <@t> montana.edu Wed Jun 6 09:03:15 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed Jun 6 09:02:53 2007 Subject: [Histonet] Question about taking test In-Reply-To: <00cb01c7a7c1$fbec90e0$d49eae18@yourxhtr8hvc4p> References: <000901c7a6f4$abf8aa60$0202a8c0@HPPav2> <466557BC.1000900@tufts.edu> <00cb01c7a7c1$fbec90e0$d49eae18@yourxhtr8hvc4p> Message-ID: <6.0.0.22.1.20070606075606.01b1fe70@gemini.msu.montana.edu> Joe brings up a very good point. However, if you are annoyed by the exam photographs which may not be high quality to begin with, take it up with ASCP. They should update or fix this part of the exam and THEY need to know what you have seen. They should also make sure the testing centers are providing good quality. It makes you wonder if the testing centers are not maintaining/adjusting their monitors for examination photograph needs. Maybe the old paper exams were not so bad after all. Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 At 04:36 PM 6/5/2007, you wrote: >I agree with Derek on this. Although when I took my HT exam, we used >parchment paper and coal, but when I took my PA exam last year, I was livid. >All the photomics where gray and pale blue. I almost blew several >questions because of the poor quality. I don't think it's so much ASCP as >it is the testing center computers may be off color. When I took my exam, >others were taking the GRE, GED, and other tests, but our exams are >probably the only one that needs critical resolution and color. My 4 cents >worth. > Derek, congratulations on passing. > >JTT >----- Original Message ----- From: "Derek Papalegis" > >To: >Cc: >Sent: Tuesday, June 05, 2007 7:31 AM >Subject: Re: [Histonet] Question about taking test > > >>I have my BS and have been in histology for over 3 years and I finally >>found the time to take the HT exam. I found the test to be flawed in many >>aspects and didn't let me accurately demonstrate my knowledge of >>histology. I knew Carson's book and Bancroft and Gamble's book from front >>to back and was extremely confident when I walked into the test.. After >>going through the test, I didn't feel as confident until I saw the tiny >>word on the screen that everyone wants to see: "PASS". The problem I had >>with the test is that histology is a very visual discipline. We are >>expected to be able to view slides and determine what stain was used and >>if that stain is adequate. The pictures on the exam were frequently >>blurry, the colors were skewed (the red in trichrome was a pale brown) >>and seemed to intentionally try to confuse the test taker instead of >>merely testing the knowledge of the person taking the exam. Over the >>years I have seen many slides that were not acceptable and could >>accurately trouble-shoot them but the slides on the exam were not even >>close to being acceptable. The ASCP set such high standards for stains >>when there was a practical portion of the exam but they seemed to drop >>the ball when they set the standards for the pictures that they use for >>the computer portion. >> >>That is my two cents... I feel that someone could know the subject >>material extremely well but then be tripped up by the seemingly >>intentionally misleading pictures and questions on the test. >> >>-- >>Derek Papalegis HT (ASCP) >>Histotechnician >>Division of Laboratory Animal Medicine >>Tufts University 136 Harrison Avenue >>Boston, MA 02111 >>phone: 617 636-2971 >>fax: 617 636-8354 >> >> >> >>Lee & Peggy Wenk wrote: >>>There are two parts to qualifying to take the HTL exam. First, the BS degree >>>with 30 hours of biology AND chemistry combined plus a minimum of 1 year's >>>experience within the last 10 years under pathologist certified by the >>>American Board of Pathology in Anatomic pathology (or the equivalent of a >>>PhD. >>> >>>Second, the experience must be within the last 10 years, and cover fixation, >>>microtomy, processing and staining. There are no other specifications. >>> >>>So the microtomy CAN be just frozen sections (or just plastics, or just >>>paraffin, etc.). >>> >>>So the staining CAN be just H&E. >>> >>>(BTW, This experience criteria is the same for the HT exam.) >>> >>>Now, with that said, the exams WILL have questions covering ALL aspects of >>>histotechnology - fixation, processing, decalcification, microtomy (frozen >>>and paraffin), all special stains, IHC, tissue ID for all tissues, enzymes >>>on the HTL, safety, equipment, lab math, etc. >>> >>>So anyone who works in just one specialty area (Mohs, derm, GI, etc.), will >>>be at a disadvantage. They will need a lot of books, atlases, and a lot of >>>time to learn all that is required. They can pass, but it will take a lot >>>more work than someone who routinely sees a variety of tissues and does a >>>variety of special stains. The last two cycles of HTL exams, 51% and 70% of >>>applicants were able to pass the HTL exam. >>> >>>Peggy A. Wenk, HTL(ASCP)SLS >>>William Beaumont Hospital >>>Royal Oak, MI 48073 >>> >>>-----Original Message----- >>>From: histonet-bounces@lists.utsouthwestern.edu >>>[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robyn >>>Vazquez >>>Sent: Monday, June 04, 2007 3:25 PM >>>To: Histonet@lists.utsouthwestern.edu >>>Subject: [Histonet] Question about taking test >>> >>> Hello, I have a question to ask. I have a co-worker that is interested in >>>taking the test and has a B.S. We work in a Mohs lab, but not an actual >>>histology lab where we would do special stains. She has been here almost 4 >>>years doing mostly Mohs surgeries, very little paraffin processing. As I am >>>understanding it, is that she would have to have 1 year experience in a >>>histology lab doing special stains and paraffin cutting and processing? >>>Since they don't make you do special stains and send them in, then she would >>>have to prove that she can perform a special stain and that is where the 1 >>>year experience comes in? >>> Thanks >>> Robyn >>>OHSU >>> >>> >>> >>> >>> >>> >>> >>> >>> >>>_______________________________________________ >>>Histonet mailing list >>>Histonet@lists.utsouthwestern.edu >>>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >>>_______________________________________________ >>>Histonet mailing list >>>Histonet@lists.utsouthwestern.edu >>>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lao_ji <@t> yahoo.com Wed Jun 6 09:03:34 2007 From: lao_ji <@t> yahoo.com (Jimmy Lao) Date: Wed Jun 6 09:03:42 2007 Subject: [Histonet] combined ISH/IHC In-Reply-To: <5CB39BCA5724F349BCB748675C6CA1A214543487@SJMEMXMB02.stjude.sjcrh.local> Message-ID: <998020.69354.qm@web30712.mail.mud.yahoo.com> Dr. Mikael Niku, I kept this email since 2004, because I thought it might be someday useful for me ( see below). now I really want to try these combined ISH/IHC, can you send me a protocol? By the way, I check your web page, it is very interesting, I like it. Hope you are still in histonelist, so you can help me. I don't have experience in Tyramide reaction. Somebody in our lab used to try PerkinElmer's, but it seems not satisfying. I like tyramide biotinylated, I think then ABC kit + DAB will get strong amplifying signal. Can anybody here share with me some tyramide with DAB staining experience and suggest the vendor you are using? Thank you very much! Jimmy Dear Tora, we are routinely doing combined ISH + IHC. The ISH is for genomic DNA, so with a RNA target this might be slightly more tricky. But RNA in tissues is surprisingly well preserved, so I think this should work. I spent a LOT of time trying to find a useful protocol for a double staining. The only generally useful way I know is to use the tyramide signal amplification system. This is how it goes: 1) Start with IHC: antigen retrieval, primary antibody, biotinylated / HRP-conjugated secondary antibody, and then the tyramide reaction. Now you have a covalently bound label (biotinylated tyramide, for example) in the tissue, so you don't need to worry about antigen destruction or stripping of bound antibodies. 2) Then perform the whole ISH procedure. 3) After you have completed the NBT/BCIP color reaction of ISH, finish the IHC (if using biotinylated tyramide, add HRP-avidin, then DAB). This protocol allows you to begin with IHC (to avoid destruction of antigens in the harsh ISH treatments) but to do the visualization step only after ISH (to avoid false negatives caused by the DAB precipitate). As additional bonus, you get signal amplification for IHC, and a nicely even DAB color intensity (nice provided that you don't need any idea of quantitation). If it's the RNA that is the more sensitive target, I guess you could do this the other way round (to use tyramide for RNA detection). The only drawbacks I can think of are: 1) A few additional incubations due to the tyramide protocol (these are quick to do, though) 2) The commercial tyramide reagents (which you need to use at least if you're doing any commercial stuff, as the technology is patented) are pretty expensive, if you're doing lots of slides. Please let me know if you would like to get a copy of our exact protocol. +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ + Mikael Niku + University of Helsinki, Dept. Basic Veterinary Sciences + URL: www.helsinki.fi/~mniku/ +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ - Mit?k? mielt? olen l?nsimaisesta sivistyksest?? Minusta se olisi erinomainen ajatus! (Gandhi) ____________________________________________________________________________________ Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. http://farechase.yahoo.com/ From lcastillos <@t> cogenics.com Wed Jun 6 09:41:41 2007 From: lcastillos <@t> cogenics.com (Castillos, Luminita) Date: Wed Jun 6 09:41:48 2007 Subject: [Histonet] Laser capture microscopy to isolate sebocytes Message-ID: Hi everybody, Does somebody have experience on laser capture microscopy to isolate human sebocytes? I have some skin punch biopsies and I need to perform LCM to isolate sebocytes for RNA purification, amplification and gene expression analysis. I am worried about: what type of staining I should perform? What kind of RNA extraction kit I should use for these particular cells and what is the RNA yield I should expect? I deeply appreciate any feedback for this particular issue. Thank you in advance. L, From Histolab <@t> lsd.uoguelph.ca Wed Jun 6 09:46:30 2007 From: Histolab <@t> lsd.uoguelph.ca (AHL Histolab) Date: Wed Jun 6 09:47:04 2007 Subject: [Histonet] solvent recycler In-Reply-To: <44780C571F28624DBB446DE55C4D733A1FE4EE@EXCHANGEBE1.carle.com> References: <001701c7a79b$9e3e4900$6501a8c0@Patsy> <44780C571F28624DBB446DE55C4D733A1FE4EE@EXCHANGEBE1.carle.com> Message-ID: <4666908602000029000096B3@lsd.uoguelph.ca> We use the CBG model and it works pretty good. >>> "Charles.Embrey" 6/5/2007 3:25 PM >>> Hi Patsy. CBG has small units that do 2.5 gal and sit on your countertop. I am sure they can set you up with a demo and then you can see first hand if it will meet your needs.. Charles Embrey PA(ASCP) Histology Manager Carle Clinic Illinois -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Tuesday, June 05, 2007 1:02 PM To: 'Histonet' Subject: [Histonet] solvent recycler I am in the market for an alcohol and xylene recycler for a small volume generator lab. I have seen some talk about CGB units. Please if you have experiences to share on these send them to me. Vendors are invited to reply, but I want an out right price for a small volume unit, I do not want to sign up for a monthly service. Are these ever sold as reconditioned or used units? Thank you, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC 12635 Montview Blvd. Ste.215 Aurora, Colorado 80045 Phone: 720-859-4060 Fax: 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From KjLand <@t> usi.edu Wed Jun 6 10:41:48 2007 From: KjLand <@t> usi.edu (Land, Kimberly J) Date: Wed Jun 6 10:40:38 2007 Subject: [Histonet] AO 820 disposable blade holder Message-ID: <5777F3052F84F14296F7B9653A51F24C11ABA5@emailnew.usi.edu> Greetings to all! We are needing some help in locating a disposable blade holder for an AO 820 microtome. We are a university that does some basic histology on a small scale. I would appreciate any help in the location of this item. I have completed some basic searches, but are out of places to look. We currently have a "loner" from a local hospital, but need to purchase one of our own. Thank you, Kim Land University of Southern Indiana 8600 University Blvd. Evansville, IN 47712 From MSHERWOOD <@t> PARTNERS.ORG Wed Jun 6 11:10:47 2007 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Wed Jun 6 11:10:59 2007 Subject: [Histonet] Laser capture microscopy to isolate sebocytes In-Reply-To: Message-ID: <1AF23D0AD12E7444A5DB083CA978B73407A31244@PHSXMB1.partners.org> Luminita, I referred you to Molecular Devices (Arcturus Division). I'm not sure what LCM instrument you are using, but they sell the kits you are interested in. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Castillos, Luminita Sent: Wednesday, June 06, 2007 10:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Laser capture microscopy to isolate sebocytes Hi everybody, Does somebody have experience on laser capture microscopy to isolate human sebocytes? I have some skin punch biopsies and I need to perform LCM to isolate sebocytes for RNA purification, amplification and gene expression analysis. I am worried about: what type of staining I should perform? What kind of RNA extraction kit I should use for these particular cells and what is the RNA yield I should expect? I deeply appreciate any feedback for this particular issue. Thank you in advance. L, _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From zacharyweil <@t> yahoo.com Wed Jun 6 11:23:34 2007 From: zacharyweil <@t> yahoo.com (Zach Weil) Date: Wed Jun 6 11:23:38 2007 Subject: [Histonet] fluoro Jade weak labelling Message-ID: <469521.74527.qm@web50603.mail.re2.yahoo.com> Histonetters, I am staning formalin-fixed paraffin embedded mouse brain with Fluoro Jade C and DAPI. These brains are from animals that had a global ischemic injury. I am getting beautiful bright fluorescence with the FJ but also some cells that are faintly fluorescent with FJ and with DAPI appear slightly shrunken and misshapen and usually have a primary process labelled. To my eye it looks very similar to the 'dark neuron' phenomenon that is often discussed with conventional H&E or Nissl techniques. I am trying to count these labelled cells and my concern is that these cells have been dead longer and thus are no longer taking up the FJ and so if I don't count them I may dramatically underestimate my injury. On the other hand I don't want to count artefactual labelling. Does anyone have a suggestion? I can send pictures if that will help. thanks very much, Zach --------------------------------- Got a little couch potato? Check out fun summer activities for kids. From bjdewe <@t> aol.com Wed Jun 6 11:35:51 2007 From: bjdewe <@t> aol.com (bjdewe@aol.com) Date: Wed Jun 6 11:36:07 2007 Subject: [Histonet] combined ISH/IHC In-Reply-To: <998020.69354.qm@web30712.mail.mud.yahoo.com> Message-ID: <8C9766FE132F15C-1264-7C76@WEBMAIL-MA14.sysops.aol.com> I would love a copy of this protocol! Sorry to bother the list but I?couldn't find the email for Mikael ;-) Cheers, Lorie Live as if you were to die tomorrow. Learn as if you were to live forever. - Gandhi - -----Original Message----- From: Jimmy Lao To: histonet@lists.utsouthwestern.edu Sent: Wed, 6 Jun 2007 6:03 am Subject: [Histonet] combined ISH/IHC Dr. Mikael Niku, kept this email since 2004, because I thought it ight be someday useful for me ( see below). now I eally want to try these combined ISH/IHC, can you end me a protocol? y the way, I check your web page, it is very nteresting, I like it. Hope you are still in istonelist, so you can help me. don't have experience in Tyramide reaction. Somebody n our lab used to try PerkinElmer's, but it seems not atisfying. like tyramide biotinylated, I think then ABC kit + AB will get strong amplifying signal. Can anybody ere share with me some tyramide with DAB staining xperience and suggest the vendor you are using? hank you very much! immy ear Tora, we are routinely doing combined ISH + IHC. The ISH is or genomic DNA, o with a RNA target this might be slightly more ricky. But RNA in issues is surprisingly well preserved, so I think his should work. I spent a LOT of time trying to find a useful protocol or a double taining. The only generally useful way I know is to se the tyramide ignal amplification system. This is how it goes: 1) Start with IHC: antigen retrieval, primary ntibody, biotinylated / RP-conjugated secondary antibody, and then the yramide reaction. Now ou have a covalently bound label (biotinylated yramide, for example) n the tissue, so you don't need to worry about ntigen destruction or tripping of bound antibodies. 2) Then perform the whole ISH procedure. 3) After you have completed the NBT/BCIP color eaction of ISH, finish he IHC (if using biotinylated tyramide, add RP-avidin, then DAB). This protocol allows you to begin with IHC (to avoid estruction of ntigens in the harsh ISH treatments) but to do the isualization step nly after ISH (to avoid false negatives caused by the AB recipitate). s additional bonus, you get signal amplification for HC, and a nicely ven DAB color intensity (nice provided that you don't eed any idea of uantitation). If it's the RNA that is the more sensitive target, I uess you could do his the other way round (to use tyramide for RNA etection). The only drawbacks I can think of are: 1) A few additional incubations due to the tyramide rotocol (these are uick to do, though) ) The commercial tyramide reagents (which you need to se at least if ou're doing any commercial stuff, as the technology s patented) are retty expensive, if you're doing lots of slides. Please let me know if you would like to get a copy of ur exact rotocol. +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Mikael Niku University of Helsinki, Dept. Basic Veterinary ciences URL: www.helsinki.fi/~mniku/ ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ - Mit?k? mielt? olen l?nsimaisesta sivistyksest?? Minusta se olisi erinomainen ajatus! (Gandhi) ___________________________________________________________________________________ ooking for a deal? Find great prices on flights and hotels with Yahoo! areChase. ttp://farechase.yahoo.com/ _______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From nadams <@t> CapeCodHealth.org Wed Jun 6 11:42:26 2007 From: nadams <@t> CapeCodHealth.org (Adams, Nancy) Date: Wed Jun 6 11:42:34 2007 Subject: [Histonet] immuno stain for H pylori Message-ID: <17A1862099540D458C8FE9380C2BC461C09389@fh2xmail.fhdomain1.capecodhealth.org> We currently do no immunostaining in our lab. Pathologists would like to start with h pylori. Looking for advice/suggestions, etc. on starting this. I slao understand we'll have to do immuno section of CAP for inspection. Thank you. Nancy Rutledge Falmouth Hospital Falmouth, MA ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential. And intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please contact the system administrator for Cape Cod Healthcare. Administrator@CapeCodHealth.org From TMcNemar <@t> lmhealth.org Wed Jun 6 12:36:30 2007 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Wed Jun 6 12:34:25 2007 Subject: [Histonet] immuno stain for H pylori In-Reply-To: <17A1862099540D458C8FE9380C2BC461C09389@fh2xmail.fhdomain1.capecodhealth.org> Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F498@lmhsmail.lmhealth.org> Well, we have done H Pylori by IHC for many years. We use the Dako Autostainer with the Dako antibody at a 1:100 dilution and do them every day. Never fails (assuming there are bugs in the control). Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Adams, Nancy Sent: Wednesday, June 06, 2007 12:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] immuno stain for H pylori We currently do no immunostaining in our lab. Pathologists would like to start with h pylori. Looking for advice/suggestions, etc. on starting this. I slao understand we'll have to do immuno section of CAP for inspection. Thank you. Nancy Rutledge Falmouth Hospital Falmouth, MA ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential. And intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please contact the system administrator for Cape Cod Healthcare. Administrator@CapeCodHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> aol.com Wed Jun 6 12:54:24 2007 From: rsrichmond <@t> aol.com (rsrichmond@aol.com) Date: Wed Jun 6 12:54:30 2007 Subject: [Histonet] Re: twin placentas CPT coding In-Reply-To: <200706061043.7c24666c7ec9c@rly-yi03.mx.aol.com> References: <200706061043.7c24666c7ec9c@rly-yi03.mx.aol.com> Message-ID: <8C9767ADA78138A-1FC8-83D8@mblk-r28.sysops.aol.com> Wanda G. Smith at Trident Medical Center asks: >>How do you bill for twin placentas that the OB has designated the cords as Baby A and Baby B? Should that be two 88307's for the designation of different"specimens"?<< The College of American Pathologists has a ruling on this that you can see at their Web site. The basic CPT code for a term or near-term placenta is 88307. If you can tell the cords and placentas of baby A and baby B apart, you can bill 88307 twice. If the information isn't available with the specimen, but you can phone and get the necessary information, that works. The final report does need to make clear that the two sides could be distinguished. Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From LSebree <@t> uwhealth.org Wed Jun 6 14:00:01 2007 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Wed Jun 6 14:00:08 2007 Subject: [Histonet] immuno stain for H pylori In-Reply-To: <17A1862099540D458C8FE9380C2BC461C09389@fh2xmail.fhdomain1.capecodhealth.org> Message-ID: Nancy, Since you're just starting out, I would go with a manual kit that includes everything you need and an antibody from the same company so that they are designed to work well together. Purchasing an automated system is probably jumping the gun a bit. But if the time comes you can query histonet and/or the histonet archives to see the pros and cons of different systems. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adams, Nancy Sent: Wednesday, June 06, 2007 11:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] immuno stain for H pylori We currently do no immunostaining in our lab. Pathologists would like to start with h pylori. Looking for advice/suggestions, etc. on starting this. I slao understand we'll have to do immuno section of CAP for inspection. Thank you. Nancy Rutledge Falmouth Hospital Falmouth, MA ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential. And intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please contact the system administrator for Cape Cod Healthcare. Administrator@CapeCodHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kimtournear <@t> yahoo.com Wed Jun 6 14:22:58 2007 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Wed Jun 6 14:23:01 2007 Subject: [Histonet] Theresa Schult Message-ID: <283643.81068.qm@web50612.mail.re2.yahoo.com> Hello, If anyone knows how I can contact Theresa Schult from Oklahoma City, please email me at this address....She will probably know me from previous names: Mann or Saunders Thanks, Kim Tournear, HT (ASCP), QIHC (ASCP) Specialists in Dermatology Mohs/Histology Supervisor Tucson, AZ --------------------------------- The fish are biting. Get more visitors on your site using Yahoo! Search Marketing. From PatPatterson <@t> mhd.com Wed Jun 6 14:57:12 2007 From: PatPatterson <@t> mhd.com (Patterson, Patricia) Date: Wed Jun 6 14:57:18 2007 Subject: [Histonet] Fat Dissolving Reagent Message-ID: Hi! I need help - we're trying to remember the name of the reagent that we had tried previously to dissolve axillary fat so the Lymph Nodes are easier to find. It had a distinctive "fragrance". Thanks Pat Patterson Methodist Dallas Medical Center 214-947-3538 patpatterson@mhd.com *********************************************************************** This electronic transmission contains information from Methodist Health System and should be considered confidential and privileged. The information contained in the above messages is intended only for the use of the individual(s) and entity(ies) named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution, or use of this information is prohibited. If you receive this transmission in error, please notify the sender immediately by return e-mail. Methodist Health System, its subsidiaries and affiliates hereby claim all applicable privileges related to the transmission of this communication. From jnocito <@t> satx.rr.com Wed Jun 6 17:20:22 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Jun 6 17:20:26 2007 Subject: [Histonet] new job Message-ID: <00df01c7a888$e01f1320$d49eae18@yourxhtr8hvc4p> This is to inform y'all that Friday, June 15 will be my last day at Pathology Reference Lab. Consequently, they are looking for a Histology manager with at least 5 years histo experience, with three years as a supervisor or lead tech. Good benefit package, new lab, new equipment, 10 techs and about 26,000 surgical cases annually. If interested, contact Mr. Mauro Guerra CEO at mguerra@pathreflab.com. JTT From mickie25 <@t> netzero.net Wed Jun 6 21:38:52 2007 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Wed Jun 6 21:39:10 2007 Subject: [Histonet] solvent recycler In-Reply-To: <001701c7a79b$9e3e4900$6501a8c0@Patsy> References: <001701c7a79b$9e3e4900$6501a8c0@Patsy> Message-ID: Hello Everyone! I have a client in Columbus, MS (east central Mississippi) looking for a histotech to staff a new physician office dermatology histology lab. If you are interested, please call the practice manager, Karen Vickers at 662-243-2435. Thanks, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net From mikael.niku <@t> helsinki.fi Thu Jun 7 02:41:03 2007 From: mikael.niku <@t> helsinki.fi (Mikael Niku) Date: Thu Jun 7 02:41:12 2007 Subject: [Histonet] combined ISH/IHC In-Reply-To: <998020.69354.qm@web30712.mail.mud.yahoo.com> References: <998020.69354.qm@web30712.mail.mud.yahoo.com> Message-ID: <4667B68F.4090306@helsinki.fi> Dear Jimmy and others, I'm still here (who would survive without Histonet!) and happy to share my protocols. However, my email address might change in the future, so please use firstname.surname[nospamplease]gmail.com to contact me directly. With best regards, Mikael -- //////////////////////////////////////////////////////////// Mikael Niku URL: mikael.nikunnakki.info University of Helsinki Dept. Basic Veterinary Sciences - Mit?k? mielt? olen l?nsimaisesta sivistyksest?? Minusta se olisi erinomainen ajatus! - Gandhi //////////////////////////////////////////////////////////// From jgutierrez <@t> precisionpath.us Thu Jun 7 05:46:14 2007 From: jgutierrez <@t> precisionpath.us (Juan Gutierrez) Date: Thu Jun 7 05:47:10 2007 Subject: [Histonet] Fat Dissolving Reagent In-Reply-To: References: Message-ID: Carnoy's? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patterson, Patricia Sent: Wednesday, June 06, 2007 2:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fat Dissolving Reagent Hi! I need help - we're trying to remember the name of the reagent that we had tried previously to dissolve axillary fat so the Lymph Nodes are easier to find. It had a distinctive "fragrance". Thanks Pat Patterson Methodist Dallas Medical Center 214-947-3538 patpatterson@mhd.com *********************************************************************** This electronic transmission contains information from Methodist Health System and should be considered confidential and privileged. The information contained in the above messages is intended only for the use of the individual(s) and entity(ies) named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution, or use of this information is prohibited. If you receive this transmission in error, please notify the sender immediately by return e-mail. Methodist Health System, its subsidiaries and affiliates hereby claim all applicable privileges related to the transmission of this communication. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joycefr <@t> frontiernet.net Thu Jun 7 07:03:19 2007 From: joycefr <@t> frontiernet.net (Joyce Friedland) Date: Thu Jun 7 07:00:21 2007 Subject: [Histonet] Syphilis Antibady Message-ID: <976E0499-84E6-4CC8-B163-BA3A6A872549@frontiernet.net> Hi Fellow Histonetters, Our pathologists would like to add a syphilis antibody to our list of available antibodies. Do any of you have a suggestion as to which one (s) we should consider? Thanks, Joyce Friedland Muhlbauer Derm-Path Lab Pittsford, NY 14534 From Marilyn.Tyler <@t> uct.ac.za Thu Jun 7 08:02:18 2007 From: Marilyn.Tyler <@t> uct.ac.za (Marilyn Tyler) Date: Thu Jun 7 08:02:38 2007 Subject: [Histonet] dysadherin Message-ID: <46681DF8.A78E.0090.0@uct.ac.za> Dear Histonetters I am posting this on behalf of a colleague. Has anyone been able to buy a monoclonal antibody to Dysadherin(human) to be used for IHC, in particular clone NCC-M53. My attempts have been unsuccessful. I have only found antibodies for IP and WB Thank you Heather/Marilyn From kimtournear <@t> yahoo.com Thu Jun 7 08:05:36 2007 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Thu Jun 7 08:05:40 2007 Subject: [Histonet] RE: Theresa Schuldt Message-ID: <710110.15632.qm@web50612.mail.re2.yahoo.com> Thanks to those of you who replied to my email....this is just what I needed.... Kim Tournear, HT (ASCP), QIHC (ASCP) Specialists in Dermatology Mohs/Histology Supervisor Tucson, AZ Kim Tournear, HT (ASCP), QIHC ( ASCP) Specialists in Dermatology Histology/Mohs Supervisor Tucson, AZ --------------------------------- Fussy? Opinionated? Impossible to please? Perfect. Join Yahoo!'s user panel and lay it on us. From Rcartun <@t> harthosp.org Thu Jun 7 09:01:35 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Jun 7 09:01:56 2007 Subject: [Histonet] immuno stain for H pylori In-Reply-To: <17A1862099540D458C8FE9380C2BC461C09389@fh2xmail.fhdomain1.capecodhealth.org> References: <17A1862099540D458C8FE9380C2BC461C09389@fh2xmail.fhdomain1.capecodhealth.org> Message-ID: <4667D780020000770000636C@gwmail.harthosp.org> To be perfectly honest, I don't think this is the best immunostain to start with. Infectious disease IHC stains need to be extremely clean and carefully validated. Also, interpretation by the pathologist can be more demanding than looking at a cytokeratin or S-100. Are your pathologists familiar with iintracytoplasmic H. pylori and will they be able to recognize it? I'm not saying it can't be done, but I would rather see you and your laboratory develop some experience with some of the more common immunostains first. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Adams, Nancy" 06/06/07 12:42 PM >>> We currently do no immunostaining in our lab. Pathologists would like to start with h pylori. Looking for advice/suggestions, etc. on starting this. I slao understand we'll have to do immuno section of CAP for inspection. Thank you. Nancy Rutledge Falmouth Hospital Falmouth, MA ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential. And intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please contact the system administrator for Cape Cod Healthcare. Administrator@CapeCodHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From Rcartun <@t> harthosp.org Thu Jun 7 09:18:02 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Jun 7 09:18:20 2007 Subject: [Histonet] Syphilis Antibady In-Reply-To: <976E0499-84E6-4CC8-B163-BA3A6A872549@frontiernet.net> References: <976E0499-84E6-4CC8-B163-BA3A6A872549@frontiernet.net> Message-ID: <4667DB5A0200007700006386@gwmail.harthosp.org> We use BioCare's polyclonal with excellent results. Keep in mind that it will cross-react with Borrelia burgdorferi (Lyme disease), although I don't think this is a problem. Keep in mind that you will need several cases for validation and control purposes to run this antibody routinely? Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> Joyce Friedland 06/07/07 8:03 AM >>> Hi Fellow Histonetters, Our pathologists would like to add a syphilis antibody to our list of available antibodies. Do any of you have a suggestion as to which one (s) we should consider? Thanks, Joyce Friedland Muhlbauer Derm-Path Lab Pittsford, NY 14534 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From jgutierrez <@t> precisionpath.us Thu Jun 7 09:21:10 2007 From: jgutierrez <@t> precisionpath.us (Juan Gutierrez) Date: Thu Jun 7 09:22:08 2007 Subject: [Histonet] Syphilis Antibady In-Reply-To: <976E0499-84E6-4CC8-B163-BA3A6A872549@frontiernet.net> References: <976E0499-84E6-4CC8-B163-BA3A6A872549@frontiernet.net> Message-ID: BioCare Medical has a nice spirochete antibody. www.biocare.net Good luck, Juan C. Gutierrez, HT(ASCP) Precision Pathology (210)646-0890 Ext. 203 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Friedland Sent: Thursday, June 07, 2007 7:03 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Syphilis Antibady Hi Fellow Histonetters, Our pathologists would like to add a syphilis antibody to our list of available antibodies. Do any of you have a suggestion as to which one (s) we should consider? Thanks, Joyce Friedland Muhlbauer Derm-Path Lab Pittsford, NY 14534 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Thu Jun 7 09:53:12 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Jun 7 09:52:50 2007 Subject: [Histonet] Syphilis Antibady In-Reply-To: <976E0499-84E6-4CC8-B163-BA3A6A872549@frontiernet.net> References: <976E0499-84E6-4CC8-B163-BA3A6A872549@frontiernet.net> Message-ID: <6.0.0.22.1.20070607085108.01b65e98@gemini.msu.montana.edu> Joyce, www.virostat-inc.com Dr. Richard Cartun recommends them for his infectious agent antibodies. He is a regular participant on Histonet. At 06:03 AM 6/7/2007, you wrote: >Hi Fellow Histonetters, >Our pathologists would like to add a syphilis antibody to our list of >available antibodies. Do any of you have a suggestion as to which one (s) >we should consider? >Thanks, >Joyce Friedland >Muhlbauer Derm-Path Lab >Pittsford, NY >14534 Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From gcallis <@t> montana.edu Thu Jun 7 10:04:42 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Jun 7 10:04:17 2007 Subject: 2nd message Re: [Histonet] Syphilis Antibady In-Reply-To: <6.0.0.22.1.20070607085108.01b65e98@gemini.msu.montana.edu> References: <976E0499-84E6-4CC8-B163-BA3A6A872549@frontiernet.net> <6.0.0.22.1.20070607085108.01b65e98@gemini.msu.montana.edu> Message-ID: <6.0.0.22.1.20070607085843.01b51148@gemini.msu.montana.edu> Dear All, Richard Cartun recommended Biocare's antibody for Treponema, but he also has used Virostat website for other infectious agent antibodies. I should have waited for his reply!!! At 08:53 AM 6/7/2007, you wrote: >Joyce, > >www.virostat-inc.com > >Dr. Richard Cartun recommends them for his infectious agent >antibodies. He is a regular participant on Histonet. > >At 06:03 AM 6/7/2007, you wrote: >>Hi Fellow Histonetters, >>Our pathologists would like to add a syphilis antibody to our list of >>available antibodies. Do any of you have a suggestion as to which one (s) >>we should consider? >>Thanks, >>Joyce Friedland >>Muhlbauer Derm-Path Lab >>Pittsford, NY >>14534 > >Gayle Callis >MT,HT,HTL(ASCP) >Research Histopathology Supervisor >Veterinary Molecular Biology >Montana State University - Bozeman >PO Box 173610 >Bozeman MT 59717-3610 > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jessgrocki <@t> yahoo.com Thu Jun 7 13:36:40 2007 From: jessgrocki <@t> yahoo.com (Jessica Piche) Date: Thu Jun 7 13:36:45 2007 Subject: [Histonet] Paraffin Block Storage Message-ID: <46304.1066.qm@web82001.mail.mud.yahoo.com> Hi Everyone, We are having a storage problem. Big surprise here! We're not allowed to store our paraffin blocks on wood pallets (fire hazard) and we have starting storing them in a paraffin block storage system (with drawers) which works nicely but we can't have them sitting on the floor. Does anyone have any ideas of what we could store them on so that its not a fire hazard? Thanks!! Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital From FUNKM <@t> mercyhealth.com Thu Jun 7 13:47:42 2007 From: FUNKM <@t> mercyhealth.com (Marcia Funk) Date: Thu Jun 7 13:48:19 2007 Subject: [Histonet] Paraffin Message-ID: <46680C7E020000AC000030C9@nodcmsngwia1.trinity-health.org> Hello, We placed metal rails on the floor and then set the cabinets on the rails. This seem to work and and is line with the fire code. mrf From hmorrow <@t> cogeco.ca Thu Jun 7 14:33:36 2007 From: hmorrow <@t> cogeco.ca (Marg & Vern Morrow) Date: Thu Jun 7 14:33:35 2007 Subject: [Histonet] remove me from the mailing list Message-ID: <000a01c7a93a$be5f4360$5650e218@OLD> Dear Histonet please remove me from the mailing list. Thanks in advance. Margaret Morrow hmorrow@cogeco.ca From Valerie.Hannen <@t> parrishmed.com Thu Jun 7 14:46:49 2007 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Thu Jun 7 14:46:56 2007 Subject: [Histonet] Fat dissolving reagent Message-ID: <5680DA93771F0C48954CC8D38425E72401AB34B1@ISMAIL.parrishmed.local> Hi Patricia, We do not use a " fat dissolving" solution to find our lymph nodes. However, we do use a great product on colonic and axillary adipose tissues, that greatly helps in finding the nodes. It is called "O-FIX", and we purchase it from Surgipath. If time in solution is not extremely critical...we leave the adipose tissue in the solution overnight at room temperature. If time is critical, we place the tissue in the solution and heat it to @ 43 degrees centigrade for approximately 3 hours with the lid loosely on the container (otherwise pressure builds up and the top can "blow" off). Hope this helps. Valerie Hannen, MLT(ASCP),HTL,SU(FL) Parrish Medical Center Titusville, Florida ************************************************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately notify us by telephone at 321-268-6167 and return the original message to us at the listed email address. Thank you From rjbuesa <@t> yahoo.com Thu Jun 7 14:59:45 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jun 7 14:59:50 2007 Subject: [Histonet] Fat dissolving reagent In-Reply-To: <5680DA93771F0C48954CC8D38425E72401AB34B1@ISMAIL.parrishmed.local> Message-ID: <723737.85923.qm@web61223.mail.yahoo.com> Patricia: I think that you are referring to the "lymph node revealing solution", with the follwoing recipe: 100% ethanol ----------2.0 L distilled water ----------0.68 L 40% formalin------------0.320 mL acetic acid --------------0.2 L Ren? J. "Hannen, Valerie" wrote: Hi Patricia, We do not use a " fat dissolving" solution to find our lymph nodes. However, we do use a great product on colonic and axillary adipose tissues, that greatly helps in finding the nodes. It is called "O-FIX", and we purchase it from Surgipath. If time in solution is not extremely critical...we leave the adipose tissue in the solution overnight at room temperature. If time is critical, we place the tissue in the solution and heat it to @ 43 degrees centigrade for approximately 3 hours with the lid loosely on the container (otherwise pressure builds up and the top can "blow" off). Hope this helps. Valerie Hannen, MLT(ASCP),HTL,SU(FL) Parrish Medical Center Titusville, Florida ************************************************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately notify us by telephone at 321-268-6167 and return the original message to us at the listed email address. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Expecting? Get great news right away with email Auto-Check. Try the Yahoo! Mail Beta. From youngir <@t> gmail.com Thu Jun 7 15:17:20 2007 From: youngir <@t> gmail.com (Ian Young) Date: Thu Jun 7 15:17:24 2007 Subject: [Histonet] Prostate cancer Message-ID: <32c00df20706071317i419bdf42s9bfa1dcfa4c07260@mail.gmail.com> Dear Histology Community. I am currently working on a project dealing with Prostate cancer. If anyone has experience working on prostate cancer, please let me know, I have a lot of questions. Anything you all could offer would be much appreciated. Basically I am looking into the Masson Trichrome as a possible technique for diagnosis. Specifics would be preferable, but again, any bit of information would be great. Thank you! -- Ian Young Histotechnology Tutor Argosy University Eagan, MN From jaimie_hk <@t> yahoo.co.uk Fri Jun 8 06:33:00 2007 From: jaimie_hk <@t> yahoo.co.uk (Jaimie Hoh) Date: Fri Jun 8 06:33:08 2007 Subject: [Histonet] job vacancies wanted Message-ID: <20070608113300.27129.qmail@web86912.mail.ukl.yahoo.com> Dear all, I have a Bachelor's degree in Biological Sciences and I have been working in a research institution for 3 years.My job consists mainly of Histological techniques , Immunohistochemistry and immunocluorescence, Immunocytochemistry and i have also learned to do DNA Extraction and purification, PCR amplification, Gel Electrophoresis, Genotyping and some Western Blotting. I am looking for employment in hospitals or research centers in London. Could you kindly email me if you kave any vacancies that match my profile? Your help would be much appreciated. Thanks, Jaimie ___________________________________________________________ Yahoo! Mail is the world's favourite email. Don't settle for less, sign up for your free account today http://uk.rd.yahoo.com/evt=44106/*http://uk.docs.yahoo.com/mail/winter07.html From pruegg <@t> ihctech.net Fri Jun 8 08:36:07 2007 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Fri Jun 8 08:34:23 2007 Subject: [Histonet] long-term storage of rat brain at -80 In-Reply-To: <000001c7a632$f0893d10$2128a8c0@mhri.edu.au> Message-ID: <200706081334.l58DYD87047853@pro12.abac.com> I go to the Hobby stores for forceps, in the Jewelry making department they sell a variety of forceps we use in the lab and they are way cheaper than purchasing for a histology supply company, mostly stainless steel though, not plastic. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David Finkelstein Sent: Sunday, June 03, 2007 5:00 PM To: histonet@lists.utsouthwestern.edu Cc: nfournier@sasktel.net Subject: [Histonet] long-term storage of rat brain at -80 Dear Neil It sounds like freeze fracture not a storage problem. Long tem freezing causes freeze drying not holes. Two suggestions. 1) Get rid the OCT. Ether suspend the brain in the mold just above the liquid nitrogen or drop the brain into the isopentane for no more than 20 sec. Then place the brain on dry ice. Keep the plastic bags on dry ice. Wrap the brains plastic file kept on dry ice. Then put them in the bags. Always transport them in or on dry ice. 2) use metal forceps. Keep the tips in dry ice. Good luck David Assoc. Professor David Finkelstein, The Mental Health Research Institute of Victoria, 155 Oak Street, Parkville, Victoria 3052 AUSTRALIA dfinkelstein@mhri.edu.au Message: 4 Date: Sat, 02 Jun 2007 20:18:30 -0600 From: Neil Fournier Subject: [Histonet] long-term storage of rat brain at -80 To: histonet@lists.utsouthwestern.edu Message-ID: <000f01c7a585$7a71f2f0$07ffc5d8@NEIL> Content-Type: text/plain; charset=iso-8859-1 I thought many of you might able to help me devise a protocol for long-term storage of flash-frozen perfused rat brains. I am sure what I am doing currently is not appropriate. Our procedure thus far is to perfuse rats with saline followed by 4% paraf ormaldehyde. Post-fix for 48 h and then cryoprotect in 30% sucrose until the brain sinks. The brains are then removed from sucrose and dried with a Kim Wipe, blocked in the coronal plane, and placed in cryomolds containing OCT. The mold is then frozen in liquid nitrogen cooled isopentane. The frozen mold is then placed in a empty ziplock bag and placed in the -80 freezer. Several months after I did this procedure and sectioned the brains, I found significant holes indicative of freezing artifact i n the tissue. Although I have had some problems in the past with my isopentane freezing protocol, I have a feeling that this issue is probably the result of storage at -80 degree C but I cannot be for certain. (I do not believe the issue is perfusio n based si Could anyone share with me their protocol or provide suggestions? Lastly, I have looked high and low but does anyone know where I can find some long plastic forceps that would be of adequate length for flash freezing? Our current one was broken accide ntally and none of us know where it came from although we are now beginning to suspect that there might be a mystical histofairy who is responsible for the often magically appearing (and sometimes disappearing) histological chemicals and equipment in la bs. Thanks again, Neil ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 43, Issue 4 *************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Fri Jun 8 08:45:49 2007 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Fri Jun 8 08:44:06 2007 Subject: What kind of plastic? Re: [Histonet] Plastic embedding In-Reply-To: <6.0.0.22.1.20070601085621.01b51348@gemini.msu.montana.edu> Message-ID: <200706081343.l58DhtR3055981@pro12.abac.com> I know of no such reference for plastic embedding and I have been doing this for almost 30 years. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Friday, June 01, 2007 8:58 AM To: zumbor@email.cs.nsw.gov.au; Histonet@lists.utsouthwestern.edu Subject: What kind of plastic? Re: [Histonet] Plastic embedding Rosalba, What kind of plastic are you planning to use? Protocols vary for different plastics. At 10:12 PM 5/31/2007, you wrote: >Hi All, >Does anyone know of a reference source either a book or website which >explains plastic embedding from start to finish and all the parphenalia >required. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Fri Jun 8 08:49:26 2007 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Fri Jun 8 08:47:43 2007 Subject: [Histonet] processing fatty tissue In-Reply-To: <41938.33094.qm@web61211.mail.yahoo.com> Message-ID: <200706081347.l58DlWa7057717@pro12.abac.com> Just curious, how do you dispose of mineral oil and what does it cost compared to xylene? Do you have to pay for haz mat shipping? Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, June 01, 2007 8:27 AM To: histonet@lists.utsouthwestern.edu Subject: Fwd: RE: Re: [Histonet] processing fatty tissue For "general enjoyment" from 2 "old timers". James McCormick and Ren? J. Rene J Buesa wrote: Date: Fri, 1 Jun 2007 07:18:42 -0700 (PDT) From: Rene J Buesa Subject: RE: Re: [Histonet] processing fatty tissue To: "McCormick, James" CC: Doug Martin , "Drew N. Mehta" , Lamar Jones , Maxim Peshkov Dear Dr. McCormick: I could not have said it better! Your description of tissue components being GENTLY awashed by succesive flows of chemicals in low gradients of different chemical actions is what exactly exists in any procedure that intercalates between the different steps leading to the substitution of tissue water with the infiltrating medium. That is the rationale behind using EthOL at increasing concentrations from a "quite shocking" 60% start (could be gentler, although TAT imposes restrictions and limitations on "tissue processing gentleness"). Once the tissues have been "completely traumatized" at the end of the dehydration, the following "chemical shock" is given by the antemedium, usually the "infamous and noxious xylene" to end with the "soothing" effect of the paraffin. That is the rationale of my procedure of eliminating the harsh xylene effect with the combination of alcohols and "liquid paraffin" = mineral oil. In my procedure by mixing ethanol + isopropanol + mineral oil, and in Maxim's modification by mixing propanol and mineral oil only. Nothing new about using propanol, it has been in use to dehydrate since the early twentieth century, and now recently has been "resurrected" as the dehydrant of choice and sole chemical between the tissue water and the wax, as in the technology in use by the Peloris instrument which uses hight "instantaneous" temperatures to "dry-out" the tissue to eliminate the propanol and leave it "open" to the wax influx. I personally would never treat a tissue sample so harshly but you know how tissue preferences go, they are like beauty, all in the beholder's eyes. For me tissue processing should be gentle though. Being an "old timer" like me, you should remember the fantastic results we used to obtain when clearing tissue with Canada balsam, the precaution to cover the floating dehydrated tissue with a piece of filter paper moisted in ethanol to prevent the tissue to dry-out, and you should also remember that the tissue itself "said" when "I am ready for infiltration", after going to the bottom of the container, before being quickly washed with benzene. Remember those days? And also how long it took to infiltrate? And how soft they were to section? Again TAT chastised all of us and dictated quicker but NOT better procedures. Mixing alcohols and mineral oil, during protocols that take the same time as with other methods that include xylene, ease infiltration and help the histotech to section better, thinner and get rid of xylene as well. Just a thought you "provoqued" with your dancing image! Ren? J. "McCormick, James" wrote: Rene J. As I am familiar with much of the "antique" instruments and tissue processing methods............your writing helps me to understand the 1860's use of essential oils to dehydrate and clear tissue for "tallow" infiltration and later paraffin waxes.....one molecule after another "holds hands and changes partners" that we might call "the line dancing of tissue processing for histotechnology" . Kindest regards, Jim, J.B.McCormick,M.D. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, June 01, 2007 7:53 AM To: Maxim Peshkov; histonet@lists.utsouthwestern.edu Subject: Fwd: Re: [Histonet] processing fatty tissue Maxim: My answer to Robert without the attachment. Ren? J. Rene J Buesa wrote: Date: Fri, 1 Jun 2007 05:48:45 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] processing fatty tissue To: Robert Chiovetti Robert: The procedure is attached. The fundament is a GENTLE dehydrant substitution by the infiltrating agent (paraffin) and ELIMINATES the use of an antemedium (xylene). The general function of an antemedium is the ability to be the "connection" between the dehydrant and the wax (paraffin) because it mixes with both. Xylene (as well as white naphtha or some other aromatic chemicals) can do this, BUT the thing is that mineral oil (MO) IS paraffin of a low molecular weight so the antemedium is not needed or a mixture of MO with alcohols constitutes the antemedium. My procedure uses EthOL to dehydrate and later the antemedium is substituted by the mixture of EthOL + Isopropanol + MO Maxim has simplified the procedure because he process manually and don't have the advantage of vacuum or pressure or agitation as I did when developed the method. So it turns out that Maxim's modification is easier and more direct; he just dehydrates with propanol and later goes into the gentle substitution with a mixture of 5 parts of propanol + 1 part of MO heated at 50?C followed by another mixture of 2 parts of propanol + 1 of MO heated also at ?C to obtain the gentle and complete infiltration of ANY type of tissue. The infiltration with MO gives the tissues a softness never achieved with any other antemedium. You will see when you try it. Maxim's method is simpler than mine and, in the long run, will be more acceptable to all histotechs and also meets the objective of eliminating xylene from the histology lab. The procedure uses 2 chemicals that are cheaper and when needing to be disposed off, the propanol can be evaporated, the used MO mixed with used paraffin and both disposed off as a solid, cutting costs also in disposal. Try it, you will like it! Ren? J. Robert Chiovetti wrote: Maxim, Rene (and Other Histonetters), That's interesting re: using either a mix of ethanol+isopropanol+mineral oil (Rene) or isopropanol+mineral oil (Maxim) for breast tissue. Could you share your recipes with us? I have a customer (derm path) who could probably benefit from this for larger and thicker skin specimens which sometimes have a lot of subcutaneous fat associated with them. Thanks in advance, if you could share the recipes! Cheers, Bob Robert (Bob) Chiovetti, Ph.D. Southwest Precision Instruments The Desert Southwest's Microscopy Resource Tucson, Arizona USA Tel./Fax 520-546-4986 www.swpinet.com Member, Arizona Small Business Association (www.asba.com) ____________________________________________________________________________ ________ Be a PS3 game guru. Get your game face on with the latest PS3 news and previews at Yahoo! Games. http://videogames.yahoo.com/platform?platform=120121 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! oneSearch: Finally, mobile search that gives answers, not web links. --------------------------------- Building a website is a piece of cake. Yahoo! Small Business gives you all the tools to get online. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. --------------------------------- Ready for the edge of your seat? Check out tonight's top picks on Yahoo! TV. --------------------------------- Be a better Globetrotter. Get better travel answers from someone who knows. Yahoo! Answers - Check it out. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From caron_fournier <@t> yahoo.ca Fri Jun 8 09:47:48 2007 From: caron_fournier <@t> yahoo.ca (caron fournier) Date: Fri Jun 8 09:47:53 2007 Subject: [Histonet] re: spurrs problem HELP!!! Message-ID: <30896.46967.qm@web35415.mail.mud.yahoo.com> Hi: I have a set of samples the were embedded in Spur's medium but for some reason it will not harden fully. It is really thick but not hard and I was wondering if anyone knows how to remove the spur's so that I can reembed them in new resin to harden them. These are really important samples and I need to salvage them if at all possible. Any help is greatly appreciated. Caron Caron Fournier, BSc, R.T. Department of Orthopaedics, Division of Orthopaedic Engineering Research, U.B.C. Get news delivered with the All new Yahoo! Mail. Enjoy RSS feeds right on your Mail page. Start today at http://mrd.mail.yahoo.com/try_beta?.intl=ca From NMargaryan <@t> childrensmemorial.org Fri Jun 8 09:57:06 2007 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Fri Jun 8 09:54:52 2007 Subject: [Histonet] (no subject) Message-ID: Dear Friends, Is anybody did PSMA staining or know expression of PSMA of the prostate cells of DU145 and PC3 lines? Any information or reference is appreciated. Regards, Naira Naira V. Margaryan, D.V.M., Ph.D. Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3394 Tel: 773-755-6570/ext-8 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 From rkpulsifer <@t> yahoo.com Fri Jun 8 10:08:46 2007 From: rkpulsifer <@t> yahoo.com (Robyn Pulsifer) Date: Fri Jun 8 10:08:50 2007 Subject: [Histonet] Cirrhosis Message-ID: <773291.2496.qm@web53212.mail.re2.yahoo.com> Dear Histonet Community- Currently I am working on a capstone project dealing with Cirrhosis of the liver. If anyone has experienced working with Cirrhosis, please contact me, I have some questions. And anything that anyone could offer me would be great. Right now I am using the Masson Trichrome as a special stain for this disease, any other suggestions that could possibly be a better stain, please let me know. Thank you Robyn Pulsifer Histology Lab Assistant Argosy University Eagan, MN --------------------------------- Building a website is a piece of cake. Yahoo! Small Business gives you all the tools to get online. From afleming <@t> mednet.ucla.edu Fri Jun 8 10:20:05 2007 From: afleming <@t> mednet.ucla.edu (Alice Fleming) Date: Fri Jun 8 10:20:11 2007 Subject: [Histonet] (no subject) Message-ID: <0B9C61BA-AB44-4584-AB04-D80512B721CC@mednet.ucla.edu> Hi, I work in a research lab and have been trying to work out a protocol for doing a double label for two proteins that really co- localize?that is, they bind each other. So far, my results make me think there?s some physical interference going on between the antibodies (and/or the attached biotin-streptavidin, etc)?I see mostly Cy3 or FITC, rarely a yellow merge. I?ve been using Perkin-Elmer?s tyramide amplification for the final step and really like it for each of these proteins individually (and in some other double label experiments where the target proteins are in separate cells or compartments). The company says I should be able to strip the first antibody-biotin-streptavidin off, leaving only the tyramide-fluorochrome bound to the tissue, then proceed to the second antibody. But they don?t have protocols for doing that. Anyone have some ideas? Alternatively, Dako suggested I use their polymer system (since the polymer is very small), with their stripper in between antibodies. They are not sure if this will work and it?s pretty expensive, so I?d like to hear if anyone has tried this. Oh, I?d like to stick to fluorescent signal, and I really have to go with paraffin-embedded tissue. Thank a lot, Alice Alice Fleming, PhD Department of Human Genetics Gonda Building 5524 University of California Los Angeles Los Angeles, CA 90095 310-267-2456 ---------------------------------------------------------- IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the recipient, please immediately notify us by return email, and delete this message from your computer. ---------------------------------------------------------- ---------------------------------------------------------- IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the recipient, please immediately notify us by return email, and delete this message from your computer. ---------------------------------------------------------- From rjbuesa <@t> yahoo.com Fri Jun 8 11:04:24 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jun 8 11:04:28 2007 Subject: [Histonet] Cirrhosis In-Reply-To: <773291.2496.qm@web53212.mail.re2.yahoo.com> Message-ID: <529024.60834.qm@web61219.mail.yahoo.com> When I worked on a cirrhosis project (in 1955!) we used Gomori's silver stain to show the liver "glitterfasern". Ren? J. Robyn Pulsifer wrote: Dear Histonet Community- Currently I am working on a capstone project dealing with Cirrhosis of the liver. If anyone has experienced working with Cirrhosis, please contact me, I have some questions. And anything that anyone could offer me would be great. Right now I am using the Masson Trichrome as a special stain for this disease, any other suggestions that could possibly be a better stain, please let me know. Thank you Robyn Pulsifer Histology Lab Assistant Argosy University Eagan, MN --------------------------------- Building a website is a piece of cake. Yahoo! Small Business gives you all the tools to get online. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ready for the edge of your seat? Check out tonight's top picks on Yahoo! TV. From JWEEMS <@t> sjha.org Fri Jun 8 12:57:41 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Jun 8 12:58:06 2007 Subject: [Histonet] Physician orders Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FF9B2D@sjhaexc02.sjha.org> Our Medical Records Dept states that they need to see the orders for special stains and immunos made by the pathologists as a physician order for billing/compliance regulations. I believe that the stain documented in the report is evidence of that order. We are attempting to find a way to make the order that the pathologist places in our computer system transfer to the hospital system, but I thought I would ask what the rest of you do regarding this issue. Thanks in advance. Happy Friday! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From gcallis <@t> montana.edu Fri Jun 8 13:00:17 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Jun 8 12:59:55 2007 Subject: [Histonet] Cirrhosis In-Reply-To: <773291.2496.qm@web53212.mail.re2.yahoo.com> References: <773291.2496.qm@web53212.mail.re2.yahoo.com> Message-ID: <6.0.0.22.1.20070608115128.01b74eb0@gemini.msu.montana.edu> A workshop director years ago suggested using a reticulin stain in conjunction with a Massons trichrome and the routine H&E stain. They did not counterstain the reticulin stain although an eosin stain would be nice OR nuclear fast red for red/black contrast. You should see correlation between reticulin stain and Masson's trichrome. Remember that Mass Tri stains ALL types of connective tissues that include basement membrane, reticulin and collagen. You could, for fun, do a Verhoeffs van Giesons for just collagen fibers too - you will have an interesting collection of stains in the end. Try to pick up adjacent sections in order to have a closer correlation within 25 um or so of each section Pick up and stain in this order (or what you prefer) 1. H&E 2. Mass Tri 3. Verhoeffs van Gieson 3. reticulin Then repeat the sequence. Good luck At 09:08 AM 6/8/2007, you wrote: >Dear Histonet Community- > > Currently I am working on a capstone project dealing with Cirrhosis of > the liver. If anyone has experienced working with Cirrhosis, please > contact me, I have some questions. And anything that anyone could offer > me would be great. Right now I am using the Masson Trichrome as a special > stain for this disease, any other suggestions that could possibly be a > better stain, please let me know. Thank you > > > > Robyn Pulsifer > Histology Lab Assistant > Argosy University > Eagan, MN > > >--------------------------------- >Building a website is a piece of cake. >Yahoo! Small Business gives you all the tools to get online. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From rjbuesa <@t> yahoo.com Fri Jun 8 13:09:01 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jun 8 13:09:06 2007 Subject: [Histonet] Physician orders In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FF9B2D@sjhaexc02.sjha.org> Message-ID: <468900.29379.qm@web61217.mail.yahoo.com> Our pathologists either requested the special stains after the initial H&E was received as part of their initial report (in the computer) and the secretaries transferred the request to the lab OR the PT wrote the requests in special forms (one for HC and the other for IHC) that were sent to the lab. The lab sent the forms to the office for billing. If the case was a "rush" the initial report was sent to the referring physician with a note of "special stains to follow" and the additional report (as an addendum) was issued on a later date. Ren? J. "Weems, Joyce" wrote: Our Medical Records Dept states that they need to see the orders for special stains and immunos made by the pathologists as a physician order for billing/compliance regulations. I believe that the stain documented in the report is evidence of that order. We are attempting to find a way to make the order that the pathologist places in our computer system transfer to the hospital system, but I thought I would ask what the rest of you do regarding this issue. Thanks in advance. Happy Friday! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Luggage? GPS? Comic books? Check out fitting gifts for grads at Yahoo! Search. From JWEEMS <@t> sjha.org Fri Jun 8 13:11:02 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Jun 8 13:11:51 2007 Subject: [Histonet] Physician orders In-Reply-To: <468900.29379.qm@web61217.mail.yahoo.com> References: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FF9B2D@sjhaexc02.sjha.org> <468900.29379.qm@web61217.mail.yahoo.com> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD320432A70C@sjhaexc02.sjha.org> Did you have to have the actual order scanned into the patient's record as a physician order? j ________________________________ From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Friday, June 08, 2007 1:09 PM To: Weems, Joyce; Histonet Subject: Re: [Histonet] Physician orders Our pathologists either requested the special stains after the initial H&E was received as part of their initial report (in the computer) and the secretaries transferred the request to the lab OR the PT wrote the requests in special forms (one for HC and the other for IHC) that were sent to the lab. The lab sent the forms to the office for billing. If the case was a "rush" the initial report was sent to the referring physician with a note of "special stains to follow" and the additional report (as an addendum) was issued on a later date. Ren? J. "Weems, Joyce" wrote: Our Medical Records Dept states that they need to see the orders for special stains and immunos made by the pathologists as a physician order for billing/compliance regulations. I believe that the stain documented in the report is evidence of that order. We are attempting to find a way to make the order that the pathologist places in our computer system transfer to the hospital system, but I thought I would ask what the rest of you do regarding this issue. Thanks in advance. Happy Friday! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Luggage? GPS? Comic books? Check out fitting gifts for grads at Yahoo! Search. Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From gcallis <@t> montana.edu Fri Jun 8 13:13:54 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Jun 8 13:13:32 2007 Subject: [Histonet] (no subject) In-Reply-To: <0B9C61BA-AB44-4584-AB04-D80512B721CC@mednet.ucla.edu> References: <0B9C61BA-AB44-4584-AB04-D80512B721CC@mednet.ucla.edu> Message-ID: <6.0.0.22.1.20070608120231.01b69ea0@gemini.msu.montana.edu> You lost me on this message, can you tell us exactly how you are doing this? Are you detecting the proteins with antibodies, in a double staining? You may have a problem with quenching (this is NOT photobleaching) where the fluorophores interfer with each other in such cloAt 09:20 AM 6/8/2007, you wrote: >Hi, I work in a research lab and have been trying to work out a >protocol for doing a double label for two proteins that really co- >localize?that is, they bind each other. So far, my results make me >think there?s some physical interference going on between the >antibodies (and/or the attached biotin-streptavidin, etc)?I see >mostly Cy3 or FITC, rarely a yellow merge. > >I?ve been using Perkin-Elmer?s tyramide amplification for the final >step and really like it for each of these proteins individually (and >in some other double label experiments where the target proteins are >in separate cells or compartments). The company says I should be >able to strip the first antibody-biotin-streptavidin off, leaving >only the tyramide-fluorochrome bound to the tissue, then proceed to >the second antibody. But they don?t have protocols for doing that. >Anyone have some ideas? > >Alternatively, Dako suggested I use their polymer system (since the >polymer is very small), with their stripper in between antibodies. >They are not sure if this will work and it?s pretty expensive, so I?d >like to hear if anyone has tried this. > >Oh, I?d like to stick to fluorescent signal, and I really have to go >with paraffin-embedded tissue. > >Thank a lot, > >Alice > > >Alice Fleming, PhD >Department of Human Genetics >Gonda Building 5524 >University of California Los Angeles >Los Angeles, CA 90095 >310-267-2456 > > > > > >---------------------------------------------------------- >IMPORTANT WARNING: This email (and any attachments) is only intended for >the use of the person or entity to which it is addressed, and may contain >information that is privileged and confidential. You, the recipient, are >obligated to maintain it in a safe, secure and confidential >manner. Unauthorized redisclosure or failure to maintain confidentiality >may subject you to federal and state penalties. If you are not the >recipient, please immediately notify us by return email, and delete this >message from your computer. >---------------------------------------------------------- > > >---------------------------------------------------------- >IMPORTANT WARNING: This email (and any attachments) is only intended for >the use of the person or entity to which it is addressed, and may contain >information that is privileged and confidential. You, the recipient, are >obligated to maintain it in a safe, secure and confidential >manner. Unauthorized redisclosure or failure to maintain confidentiality >may subject you to federal and state penalties. If you are not the >recipient, please immediately notify us by return email, and delete this >message from your computer. >---------------------------------------------------------- > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From NSEARCY <@t> swmail.sw.org Fri Jun 8 13:58:48 2007 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Fri Jun 8 13:59:02 2007 Subject: [Histonet] Bone Saw Message-ID: I have been asked to obtain a saw for cutting bone. We have the Mar-Med presently but its not "big" enough for larger bones. In the past the institution had a band saw but found it unsafe. Any suggestions? Thanks Nita Searcy Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 254-724-2438 -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division END:VCARD From rjbuesa <@t> yahoo.com Fri Jun 8 14:03:58 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jun 8 14:04:02 2007 Subject: [Histonet] Bone Saw In-Reply-To: Message-ID: <964347.28081.qm@web61213.mail.yahoo.com> A band saw is the ideal instrument. Just try to "protect (cover)" the saw and use precaution. Also buy the smallest band saw capable of doing the job. I don't think you will find anything better (or cheaper) for large bones. Ren? J. Nita Searcy wrote: I have been asked to obtain a saw for cutting bone. We have the Mar-Med presently but its not "big" enough for larger bones. In the past the institution had a band saw but found it unsafe. Any suggestions? Thanks Nita Searcy Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 254-724-2438 BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division END:VCARD _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- 8:00? 8:25? 8:40? Find a flick in no time with theYahoo! Search movie showtime shortcut. From mpence <@t> grhs.net Fri Jun 8 14:17:33 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Fri Jun 8 14:17:38 2007 Subject: [Histonet] Bone Saw In-Reply-To: <964347.28081.qm@web61213.mail.yahoo.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C620@IS-E2K3.grhs.net> Try this saw. Works great for any size bone and you can't find one cheaper. http://www.thermo.com/com/cda/product/detail/1,,10121267,00.html Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, June 08, 2007 2:04 PM To: Nita Searcy; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Bone Saw A band saw is the ideal instrument. Just try to "protect (cover)" the saw and use precaution. Also buy the smallest band saw capable of doing the job. I don't think you will find anything better (or cheaper) for large bones. Ren? J. Nita Searcy wrote: I have been asked to obtain a saw for cutting bone. We have the Mar-Med presently but its not "big" enough for larger bones. In the past the institution had a band saw but found it unsafe. Any suggestions? Thanks Nita Searcy Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 254-724-2438 BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division END:VCARD _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- 8:00? 8:25? 8:40? Find a flick in no time with theYahoo! Search movie showtime shortcut. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jlinda <@t> ces.clemson.edu Fri Jun 8 14:29:10 2007 From: jlinda <@t> ces.clemson.edu (Linda Jenkins) Date: Fri Jun 8 14:29:22 2007 Subject: [Histonet] re: spurrs problem HELP!!! Message-ID: <6.2.3.4.2.20070608152114.03187260@mailhost.ces.clemson.edu> Caron, Many years ago(before google searches were common place) I had a similar Spurr's experience. I was told at the time that there was no retrieval method available...my sample was garbage. I have never used Spurr's since then;however, using a modern day google search, I found 2 articles that might help. Go to: http://www.emsdiasum.com/microscopy/technical/techtips/re-embedding.aspx Maybe I have been unfair to Spurr's. Regards, Linda Jenkins Bioengineering Clemson University From gu.lang <@t> gmx.at Fri Jun 8 14:38:54 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Jun 8 14:39:02 2007 Subject: AW: [Histonet] Cirrhosis In-Reply-To: <773291.2496.qm@web53212.mail.re2.yahoo.com> Message-ID: <004601c7aa04$a6a26480$6412a8c0@dielangs.at> Our panel for liverdiagnosis is: Reticulin stain - Gomori (architecture) PAS and Amylase-PAS (glycogen and architecture, mallory bodies) Prussian Blue (iron) Hall stain (bile) Trichrom Gomori(onestep-trichrom with anilinblue and chromotrop 2R - ready made by Sigma-Aldrich; collagen fibers, mallory-bodies) Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Robyn Pulsifer Gesendet: Freitag, 08. Juni 2007 17:09 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Cirrhosis Dear Histonet Community- Currently I am working on a capstone project dealing with Cirrhosis of the liver. If anyone has experienced working with Cirrhosis, please contact me, I have some questions. And anything that anyone could offer me would be great. Right now I am using the Masson Trichrome as a special stain for this disease, any other suggestions that could possibly be a better stain, please let me know. Thank you Robyn Pulsifer Histology Lab Assistant Argosy University Eagan, MN --------------------------------- Building a website is a piece of cake. Yahoo! Small Business gives you all the tools to get online. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From afleming <@t> mednet.ucla.edu Fri Jun 8 15:47:55 2007 From: afleming <@t> mednet.ucla.edu (Alice Fleming) Date: Fri Jun 8 15:48:04 2007 Subject: [Histonet] (no subject) double label In-Reply-To: <6.0.0.22.1.20070608120231.01b69ea0@gemini.msu.montana.edu> References: <0B9C61BA-AB44-4584-AB04-D80512B721CC@mednet.ucla.edu> <6.0.0.22.1.20070608120231.01b69ea0@gemini.msu.montana.edu> Message-ID: Hello Gayle, Sorry about the confusion. Yes, I'm detecting the proteins with antibodies in a double staining. I'd like to try completing staining of the first antibody the way I have been doing it (unmasking with citrate buffer, quenching, primary antibody, biotinylated secondary antibody, HRP-conjugated streptavidin, FITC- conjugated tyramide), then somehow strip that primary antibody before continuing a similar process with the second primary antibody and Cy3. Perkin Elmer says the FITC-tyramide will be covalently bound and should stay on through subsequent steps. Do you know a good way to strip the first antibody? Also, could you tell me more about fluorophores interfering with each other (part of your message got cut off)? Thanks, Alice On Jun 8, 2007, at 11:13 AM, Gayle Callis wrote: > > You lost me on this message, can you tell us exactly how you are > doing this? Are you detecting the proteins with antibodies, in a > double staining? > > You may have a problem with quenching (this is NOT photobleaching) > where the fluorophores interfer with each other in such cloAt 09:20 > AM 6/8/2007, you wrote: >> Hi, I work in a research lab and have been trying to work out a >> protocol for doing a double label for two proteins that really co- >> localize?that is, they bind each other. So far, my results make me >> think there?s some physical interference going on between the >> antibodies (and/or the attached biotin-streptavidin, etc)?I see >> mostly Cy3 or FITC, rarely a yellow merge. >> >> I?ve been using Perkin-Elmer?s tyramide amplification for the final >> step and really like it for each of these proteins individually (and >> in some other double label experiments where the target proteins are >> in separate cells or compartments). The company says I should be >> able to strip the first antibody-biotin-streptavidin off, leaving >> only the tyramide-fluorochrome bound to the tissue, then proceed to >> the second antibody. But they don?t have protocols for doing that. >> Anyone have some ideas? >> >> Alternatively, Dako suggested I use their polymer system (since the >> polymer is very small), with their stripper in between antibodies. >> They are not sure if this will work and it?s pretty expensive, so I?d >> like to hear if anyone has tried this. >> >> Oh, I?d like to stick to fluorescent signal, and I really have to go >> with paraffin-embedded tissue. >> >> Thank a lot, >> >> Alice >> >> >> Alice Fleming, PhD >> Department of Human Genetics >> Gonda Building 5524 >> University of California Los Angeles >> Los Angeles, CA 90095 >> 310-267-2456 >> >> >> >> >> >> ---------------------------------------------------------- >> IMPORTANT WARNING: This email (and any attachments) is only >> intended for the use of the person or entity to which it is >> addressed, and may contain information that is privileged and >> confidential. You, the recipient, are obligated to maintain it in >> a safe, secure and confidential manner. Unauthorized redisclosure >> or failure to maintain confidentiality may subject you to federal >> and state penalties. If you are not the recipient, please >> immediately notify us by return email, and delete this message >> from your computer. >> ---------------------------------------------------------- >> >> >> ---------------------------------------------------------- >> IMPORTANT WARNING: This email (and any attachments) is only >> intended for the use of the person or entity to which it is >> addressed, and may contain information that is privileged and >> confidential. You, the recipient, are obligated to maintain it in >> a safe, secure and confidential manner. Unauthorized redisclosure >> or failure to maintain confidentiality may subject you to federal >> and state penalties. If you are not the recipient, please >> immediately notify us by return email, and delete this message >> from your computer. >> ---------------------------------------------------------- >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > > > Alice Fleming, PhD Department of Human Genetics Gonda Building 5524 University of California Los Angeles Los Angeles, CA 90095 310-267-2456 ---------------------------------------------------------- IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the recipient, please immediately notify us by return email, and delete this message from your computer. ---------------------------------------------------------- ---------------------------------------------------------- IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the recipient, please immediately notify us by return email, and delete this message from your computer. ---------------------------------------------------------- From JMyers1 <@t> aol.com Fri Jun 8 17:44:51 2007 From: JMyers1 <@t> aol.com (JMyers1@aol.com) Date: Fri Jun 8 17:45:07 2007 Subject: [Histonet] Prostate cancer Message-ID: Ian: Although trichrome stains can be used as an aid in the for diagnosis/evaluation of prostate tumors, the most commonly accepted methods in use today involve immunohistochemical (IHC) stains for such 'markers' as p63, high molecular weight cytokeratin (and other CK's), and AMACR/racemeace/P504S. I have extensive experience performing IHC procedures for these antigens, and if can offer you any assistance, please let me know... Best Wishes, Joe Myers, M.S., CT(ASCP) ********************************************* Message: 6 Date: Thu, 7 Jun 2007 15:17:20 -0500 From: "Ian Young" Subject: [Histonet] Prostate cancer To: _histonet@lists.utsouthwestern.edu_ (mailto:histonet@lists.utsouthwestern.edu) Dear Histology Community. I am currently working on a project dealing with Prostate cancer. If anyone has experience working on prostate cancer, please let me know, I have a lot of questions. Anything you all could offer would be much appreciated. Basically I am looking into the Masson Trichrome as a possible technique for diagnosis. Specifics would be preferable, but again, any bit of information would be great. Thank you! Ian Young Histotechnology Tutor Argosy University Eagan, MN ************************************** See what's free at http://www.aol.com. From akemiat3377 <@t> yahoo.com Fri Jun 8 17:57:10 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Fri Jun 8 17:57:13 2007 Subject: [Histonet] Prostate cancer In-Reply-To: Message-ID: <439739.51203.qm@web31301.mail.mud.yahoo.com> And what about the PIN 4 Cocktail? I do believe Dr. De Marzo from Johns Hopkins has been using it for some time with great success. He is quite the prostate expert. Akemi Allison-Tacha --- JMyers1@aol.com wrote: > > Ian: > Although trichrome stains can be used as an aid in > the for > diagnosis/evaluation of prostate tumors, the most > commonly accepted methods in use today involve > immunohistochemical (IHC) stains for such 'markers' > as p63, high molecular > weight cytokeratin (and other CK's), and > AMACR/racemeace/P504S. I have > extensive experience performing IHC procedures for > these antigens, and if can offer > you any assistance, please let me know... > Best Wishes, > Joe Myers, M.S., CT(ASCP) > > ********************************************* > > Message: 6 > Date: Thu, 7 Jun 2007 15:17:20 -0500 > From: "Ian Young" > Subject: [Histonet] Prostate cancer > To: _histonet@lists.utsouthwestern.edu_ > (mailto:histonet@lists.utsouthwestern.edu) > > Dear Histology Community. > I am currently working on a project dealing with > Prostate cancer. If anyone > has experience working on prostate cancer, please > let me know, I have a lot > of questions. Anything you all could offer would be > much appreciated. > Basically I am looking into the Masson Trichrome as > a possible technique for > diagnosis. Specifics would be preferable, but again, > any bit of information > would be great. > > Thank you! > > Ian Young > Histotechnology Tutor > Argosy University > Eagan, MN > > > > > > ************************************** See what's > free at http://www.aol.com. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From sbanwait <@t> buckinstitute.org Sat Jun 9 13:00:28 2007 From: sbanwait <@t> buckinstitute.org (Surita Banwait) Date: Sat Jun 9 13:00:52 2007 Subject: [Histonet] temporary entry level job Novato CA Message-ID: Hi There, We are in need of a Temp for our Morphology Department ASAP. Here is the ad. Please contact me if you are interested. Temporary Technician - Morphology Core 35-40 hours/week @$17/hr. ASAP to November 15, 2007 The Buck Institute for Age Research in Novato, CA is looking for an upper-class or recent college graduate needed to assist in biomedical research on Alzheimer's, Parkinson's, Huntington's, and/or ALS. Training will include tissue preparation, sectioning, and staining techniques for brightfield, fluorescence, or confocal with state-of-the-art computerized video imaging. Experience in ANY of these categories is a plus! Courses in histology, biochemistry, biology, chemistry, and/ or physiology required. Proficiency with Microsoft, Macintosh, Adobe Photoshop or Windows NT software a plus. Ideal for those needing temporary work , students, and those interested in a possible career change from industry to university-type setting. From billingconsultants <@t> yahoo.com Sat Jun 9 14:30:08 2007 From: billingconsultants <@t> yahoo.com (Billing Consultants, LLC) Date: Sat Jun 9 15:57:13 2007 Subject: [Histonet] Physician Order Message-ID: <786871.35246.qm@web54206.mail.re2.yahoo.com> Joyce, The documentation in the pathology report is sufficient to document the physicians order. To be compliant, they should bill off the pathology report - not the order forms. If it's not documented on the pathology report, they can't bill it even if an order was placed. Kindest regards, Louri www.billingconsultants.net histonet-request@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Physician orders (Weems, Joyce) 2. Re: Cirrhosis (Gayle Callis) 3. Re: Physician orders (Rene J Buesa) 4. RE: Physician orders (Weems, Joyce) 5. Re: (no subject) (Gayle Callis) 6. Bone Saw (Nita Searcy) 7. Re: Bone Saw (Rene J Buesa) 8. RE: Bone Saw (Mike Pence) 9. re: spurrs problem HELP!!! (Linda Jenkins) 10. AW: [Histonet] Cirrhosis (Gudrun Lang) 11. Re: (no subject) double label (Alice Fleming) 12. Re: Prostate cancer (JMyers1@aol.com) 13. Re: Prostate cancer (Akemi Allison-Tacha) ---------------------------------------------------------------------- Message: 1 Date: Fri, 8 Jun 2007 13:57:41 -0400 From: "Weems, Joyce" Subject: [Histonet] Physician orders To: "Histonet" Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FF9B2D@sjhaexc02.sjha.org> Content-Type: text/plain; charset="utf-8" Our Medical Records Dept states that they need to see the orders for special stains and immunos made by the pathologists as a physician order for billing/compliance regulations. I believe that the stain documented in the report is evidence of that order. We are attempting to find a way to make the order that the pathologist places in our computer system transfer to the hospital system, but I thought I would ask what the rest of you do regarding this issue. Thanks in advance. Happy Friday! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. ------------------------------ Message: 2 Date: Fri, 08 Jun 2007 12:00:17 -0600 From: Gayle Callis Subject: Re: [Histonet] Cirrhosis To: Robyn Pulsifer , Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20070608115128.01b74eb0@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed A workshop director years ago suggested using a reticulin stain in conjunction with a Massons trichrome and the routine H&E stain. They did not counterstain the reticulin stain although an eosin stain would be nice OR nuclear fast red for red/black contrast. You should see correlation between reticulin stain and Masson's trichrome. Remember that Mass Tri stains ALL types of connective tissues that include basement membrane, reticulin and collagen. You could, for fun, do a Verhoeffs van Giesons for just collagen fibers too - you will have an interesting collection of stains in the end. Try to pick up adjacent sections in order to have a closer correlation within 25 um or so of each section Pick up and stain in this order (or what you prefer) 1. H&E 2. Mass Tri 3. Verhoeffs van Gieson 3. reticulin Then repeat the sequence. Good luck At 09:08 AM 6/8/2007, you wrote: >Dear Histonet Community- > > Currently I am working on a capstone project dealing with Cirrhosis of > the liver. If anyone has experienced working with Cirrhosis, please > contact me, I have some questions. And anything that anyone could offer > me would be great. Right now I am using the Masson Trichrome as a special > stain for this disease, any other suggestions that could possibly be a > better stain, please let me know. Thank you > > > > Robyn Pulsifer > Histology Lab Assistant > Argosy University > Eagan, MN > > >--------------------------------- >Building a website is a piece of cake. >Yahoo! Small Business gives you all the tools to get online. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 ------------------------------ Message: 3 Date: Fri, 8 Jun 2007 11:09:01 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Physician orders To: "Weems, Joyce" , Histonet Message-ID: <468900.29379.qm@web61217.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Our pathologists either requested the special stains after the initial H&E was received as part of their initial report (in the computer) and the secretaries transferred the request to the lab OR the PT wrote the requests in special forms (one for HC and the other for IHC) that were sent to the lab. The lab sent the forms to the office for billing. If the case was a "rush" the initial report was sent to the referring physician with a note of "special stains to follow" and the additional report (as an addendum) was issued on a later date. Ren? J. "Weems, Joyce" wrote: Our Medical Records Dept states that they need to see the orders for special stains and immunos made by the pathologists as a physician order for billing/compliance regulations. I believe that the stain documented in the report is evidence of that order. We are attempting to find a way to make the order that the pathologist places in our computer system transfer to the hospital system, but I thought I would ask what the rest of you do regarding this issue. Thanks in advance. Happy Friday! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Luggage? GPS? Comic books? Check out fitting gifts for grads at Yahoo! Search. ------------------------------ Message: 4 Date: Fri, 8 Jun 2007 14:11:02 -0400 From: "Weems, Joyce" Subject: RE: [Histonet] Physician orders To: "Rene J Buesa" , "Histonet" Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD320432A70C@sjhaexc02.sjha.org> Content-Type: text/plain; charset="utf-8" Did you have to have the actual order scanned into the patient's record as a physician order? j ________________________________ From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Friday, June 08, 2007 1:09 PM To: Weems, Joyce; Histonet Subject: Re: [Histonet] Physician orders Our pathologists either requested the special stains after the initial H&E was received as part of their initial report (in the computer) and the secretaries transferred the request to the lab OR the PT wrote the requests in special forms (one for HC and the other for IHC) that were sent to the lab. The lab sent the forms to the office for billing. If the case was a "rush" the initial report was sent to the referring physician with a note of "special stains to follow" and the additional report (as an addendum) was issued on a later date. Ren?? J. "Weems, Joyce" wrote: Our Medical Records Dept states that they need to see the orders for special stains and immunos made by the pathologists as a physician order for billing/compliance regulations. I believe that the stain documented in the report is evidence of that order. We are attempting to find a way to make the order that the pathologist places in our computer system transfer to the hospital system, but I thought I would ask what the rest of you do regarding this issue. Thanks in advance. Happy Friday! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Luggage? GPS? Comic books? Check out fitting gifts for grads at Yahoo! Search. Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. ------------------------------ Message: 5 Date: Fri, 08 Jun 2007 12:13:54 -0600 From: Gayle Callis Subject: Re: [Histonet] (no subject) To: Alice Fleming , Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20070608120231.01b69ea0@gemini.msu.montana.edu> Content-Type: text/plain; charset="iso-8859-1"; format=flowed You lost me on this message, can you tell us exactly how you are doing this? Are you detecting the proteins with antibodies, in a double staining? You may have a problem with quenching (this is NOT photobleaching) where the fluorophores interfer with each other in such cloAt 09:20 AM 6/8/2007, you wrote: >Hi, I work in a research lab and have been trying to work out a >protocol for doing a double label for two proteins that really co- >localize?that is, they bind each other. So far, my results make me >think there?s some physical interference going on between the >antibodies (and/or the attached biotin-streptavidin, etc)?I see >mostly Cy3 or FITC, rarely a yellow merge. > >I?ve been using Perkin-Elmer?s tyramide amplification for the final >step and really like it for each of these proteins individually (and >in some other double label experiments where the target proteins are >in separate cells or compartments). The company says I should be >able to strip the first antibody-biotin-streptavidin off, leaving >only the tyramide-fluorochrome bound to the tissue, then proceed to >the second antibody. But they don?t have protocols for doing that. >Anyone have some ideas? > >Alternatively, Dako suggested I use their polymer system (since the >polymer is very small), with their stripper in between antibodies. >They are not sure if this will work and it?s pretty expensive, so I?d >like to hear if anyone has tried this. > >Oh, I?d like to stick to fluorescent signal, and I really have to go >with paraffin-embedded tissue. > >Thank a lot, > >Alice > > >Alice Fleming, PhD >Department of Human Genetics >Gonda Building 5524 >University of California Los Angeles >Los Angeles, CA 90095 >310-267-2456 > > > > > >---------------------------------------------------------- >IMPORTANT WARNING: This email (and any attachments) is only intended for >the use of the person or entity to which it is addressed, and may contain >information that is privileged and confidential. You, the recipient, are >obligated to maintain it in a safe, secure and confidential >manner. Unauthorized redisclosure or failure to maintain confidentiality >may subject you to federal and state penalties. If you are not the >recipient, please immediately notify us by return email, and delete this >message from your computer. >---------------------------------------------------------- > > >---------------------------------------------------------- >IMPORTANT WARNING: This email (and any attachments) is only intended for >the use of the person or entity to which it is addressed, and may contain >information that is privileged and confidential. You, the recipient, are >obligated to maintain it in a safe, secure and confidential >manner. Unauthorized redisclosure or failure to maintain confidentiality >may subject you to federal and state penalties. If you are not the >recipient, please immediately notify us by return email, and delete this >message from your computer. >---------------------------------------------------------- > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 ------------------------------ Message: 6 Date: Fri, 08 Jun 2007 13:58:48 -0500 From: "Nita Searcy" Subject: [Histonet] Bone Saw To: Message-ID: Content-Type: text/plain; charset="us-ascii" I have been asked to obtain a saw for cutting bone. We have the Mar-Med presently but its not "big" enough for larger bones. In the past the institution had a band saw but found it unsafe. Any suggestions? Thanks Nita Searcy Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 254-724-2438 -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division END:VCARD ------------------------------ Message: 7 Date: Fri, 8 Jun 2007 12:03:58 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Bone Saw To: Nita Searcy , histonet@lists.utsouthwestern.edu Message-ID: <964347.28081.qm@web61213.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 A band saw is the ideal instrument. Just try to "protect (cover)" the saw and use precaution. Also buy the smallest band saw capable of doing the job. I don't think you will find anything better (or cheaper) for large bones. Ren? J. Nita Searcy wrote: I have been asked to obtain a saw for cutting bone. We have the Mar-Med presently but its not "big" enough for larger bones. In the past the institution had a band saw but found it unsafe. Any suggestions? Thanks Nita Searcy Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 254-724-2438 BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division END:VCARD _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- 8:00? 8:25? 8:40? Find a flick in no time with theYahoo! Search movie showtime shortcut. ------------------------------ Message: 8 Date: Fri, 8 Jun 2007 14:17:33 -0500 From: "Mike Pence" Subject: RE: [Histonet] Bone Saw To: "Rene J Buesa" , "Nita Searcy" , Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C620@IS-E2K3.grhs.net> Content-Type: text/plain; charset="iso-8859-1" Try this saw. Works great for any size bone and you can't find one cheaper. http://www.thermo.com/com/cda/product/detail/1,,10121267,00.html Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, June 08, 2007 2:04 PM To: Nita Searcy; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Bone Saw A band saw is the ideal instrument. Just try to "protect (cover)" the saw and use precaution. Also buy the smallest band saw capable of doing the job. I don't think you will find anything better (or cheaper) for large bones. Ren? J. Nita Searcy wrote: I have been asked to obtain a saw for cutting bone. We have the Mar-Med presently but its not "big" enough for larger bones. In the past the institution had a band saw but found it unsafe. Any suggestions? Thanks Nita Searcy Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 254-724-2438 BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division END:VCARD _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- 8:00? 8:25? 8:40? Find a flick in no time with theYahoo! Search movie showtime shortcut. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Fri, 08 Jun 2007 15:29:10 -0400 From: Linda Jenkins Subject: [Histonet] re: spurrs problem HELP!!! To: histonet@lists.utsouthwestern.edu Message-ID: <6.2.3.4.2.20070608152114.03187260@mailhost.ces.clemson.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Caron, Many years ago(before google searches were common place) I had a similar Spurr's experience. I was told at the time that there was no retrieval method available...my sample was garbage. I have never used Spurr's since then;however, using a modern day google search, I found 2 articles that might help. Go to: http://www.emsdiasum.com/microscopy/technical/techtips/re-embedding.aspx Maybe I have been unfair to Spurr's. === message truncated === --------------------------------- Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. From santiagom <@t> nei.nih.gov Sun Jun 10 18:42:22 2007 From: santiagom <@t> nei.nih.gov (Santiago, Maribel (NIH/NEI) [C]) Date: Sun Jun 10 18:42:29 2007 Subject: [Histonet] Question? References: <773291.2496.qm@web53212.mail.re2.yahoo.com> Message-ID: Dear Histonet, Does anyone out there graduated from AFIP knows what is the school code to apply to ASCP? I have a co-worker who needs this and the person who can give her this number is on maternity leave until late august possible September. The person they left in charge is a military who says he doesn't know and told her she has to wait until the person in charge comes back. My coworker doesn't have that much time to apply. Any help would be greatly appreciated. Thanks, Maribel Santiago- Maysonet, HTL (ASCP) From barry_m <@t> ozemail.com.au Sun Jun 10 23:51:27 2007 From: barry_m <@t> ozemail.com.au (Barry Madigan) Date: Sun Jun 10 23:51:38 2007 Subject: [Histonet] Melanin- A in Tasmanian Devil Message-ID: <000001c7abe4$2d7e65e0$887b31a0$@com.au> I have been doing some Immunohistochemistry on tumours from Tasmanian Devils. Did a Melanin -A (Clone A103) which showed nuclear staining. I was wondering if anyone has noticed nuclear staining with this antibody. Could it be cross reactivity? Regards Barry Madigan Immunohistochemistry QHPS-RBH Royal Brisbane Hospital Australia From sarah <@t> als.co.nz Mon Jun 11 01:14:47 2007 From: sarah <@t> als.co.nz (Sarah O'Bryan) Date: Mon Jun 11 00:15:50 2007 Subject: [Histonet] Cyclin D1 antibody from Cell Marque user experiences Message-ID: <03568ED922E71549B7C3D55569A8C089127B2D@dchq.als.local> Hello all, I would like some information or feedback on how people find different Cyclin D1 antibodies? I can see from some previous messages that it can be a difficult antibody to optimize... I am particularly interested in Cell Marque's users, as we have a customer who would like to know of user experiences with this antibody before she decides whether to trial or not. If you use Cyclin D1 in your laboratory, would you please let me know what clone you use, who manufactures it, and what you think of it? What is the leading antibody on the market? Thanks for your help. Sarah From barry_m <@t> ozemail.com.au Mon Jun 11 01:17:11 2007 From: barry_m <@t> ozemail.com.au (Barry Madigan) Date: Mon Jun 11 01:17:20 2007 Subject: [Histonet] Cyclin D1 antibody from Cell Marque user experiences In-Reply-To: <03568ED922E71549B7C3D55569A8C089127B2D@dchq.als.local> References: <03568ED922E71549B7C3D55569A8C089127B2D@dchq.als.local> Message-ID: <000501c7abf0$2765eb40$7631c1c0$@com.au> Hi Sarah, We use Cyclin D1 Clone SP4 from Lab Vision at a dilution of 1:50 using high pH heat retrieval. This is on a BondMax immunostainer with the Polymer Refine detection system. Excellent results. Regards Barry Madigan Immunohistochemistry QHPS-RBH Royal Brisbane Hospital Australia -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah O'Bryan Sent: Monday, 11 June 2007 4:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cyclin D1 antibody from Cell Marque user experiences Hello all, I would like some information or feedback on how people find different Cyclin D1 antibodies? I can see from some previous messages that it can be a difficult antibody to optimize... I am particularly interested in Cell Marque's users, as we have a customer who would like to know of user experiences with this antibody before she decides whether to trial or not. If you use Cyclin D1 in your laboratory, would you please let me know what clone you use, who manufactures it, and what you think of it? What is the leading antibody on the market? Thanks for your help. Sarah _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcbook <@t> gmail.com Mon Jun 11 03:28:15 2007 From: jcbook <@t> gmail.com (J C) Date: Mon Jun 11 03:28:20 2007 Subject: [Histonet] SRY for liver Message-ID: <52cb0f130706110128i500e6419j808de11e1b260909@mail.gmail.com> Dear histonetters! I have really a big problem with ISH on rat liver! I need to do ISH of SRY gene on genomic DNA to distinguish between male and female hepatocytes. Everything I do doesn't work, I have read a lot of articles, tried everything: frozen and paraffin sections, different proteinases, but it doesn't work. Maybe somebody works with SRY on liver cell? Please, it is about half a year that I try, I am absolutely desperate. Thanks, Julie Carmel. From santosma <@t> uni-mainz.de Mon Jun 11 04:57:57 2007 From: santosma <@t> uni-mainz.de (Marina Santos) Date: Mon Jun 11 04:57:44 2007 Subject: [Histonet] Biomaterial embedding in paraffin - sections detach from the slides Message-ID: <200706110957.l5B9vU84014051@server12.ukmainz.de> Hi, I am having problems with sections sliding off from the slide even from the slides with positive charges (treated for frozen sections). However, I am not working with human tissue. I culture cells in a scaffold material, that is a biomaterial made from a blend of starch with polycaprolactone (30/70 wt%), and then I embed it in paraffin. The problem is that after deparaffinization and during the washing steps the material detaches from the slides. This also happens with frozen sections of this biomaterial, therefore I am thinking that the problem is related with the chemistry nature of my biomaterial. Besides being hydrophobic, the biomaterial also has a neutral charge. With human tissue there isn't such big problem because it is hydrophilic, i.e., the same polarity as the glass and besides that is negatively charged so strong interaction will be established with the positively charged glass slides. I already eliminated some of the most common situations that could justify the detachment of sections from glass slides: 1) paraffin sections were very well dried 2) I tried buffers for antigen retrieval with different pH (citrate and EDTA). I even avoided the step of antigen retrieval but even so the sections were detaching 3) The biomaterial was fixed for long time but I also tried short time 4) There were no wrinkles in the sections Therefore, I am really convinced that the problem relies in the chemistry of the biomaterial. Do you have any suggestion how to go around this problem? Any glass slide with a treatment that might improve the adhesion of the sections? Or any other suggestion that might not be related with material chemistry? Thank you in advance. Marina Santos Department of Polymer Engineering University of Minho Portugal From bakerj <@t> umich.edu Mon Jun 11 07:11:40 2007 From: bakerj <@t> umich.edu (John Baker) Date: Mon Jun 11 07:18:31 2007 Subject: [Histonet] Question? In-Reply-To: References: <773291.2496.qm@web53212.mail.re2.yahoo.com> Message-ID: <368a991316d90de9d84f98c1a5b7f488@umich.edu> On Jun 10, 2007, at 7:42 PM, Santiago, Maribel (NIH/NEI) [C] wrote: > Dear Histonet, > > Does anyone out there graduated from AFIP knows what is the school > code to apply to ASCP? I have a co-worker who needs this and the > person who can give her this number is on maternity leave until late > august possible September. The person they left in charge is a > military who says he doesn't know and told her she has to wait until > the person in charge comes back. My coworker doesn't have that much > time to apply. Any help would be greatly appreciated. > > Thanks, > > Maribel Santiago- Maysonet, HTL (ASCP) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > John A. Baker The University of Michigan Orthopaedic Research Laboratories Histology Unit 109 Zina Pitcher Place, 2218 BSRB Ann Arbor, MI 48109-2200 734-936-1635 From doug <@t> ppspath.com Mon Jun 11 08:33:56 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Mon Jun 11 07:33:30 2007 Subject: [Histonet] Question? In-Reply-To: Message-ID: I believe this is the code. 906002 Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Santiago, Maribel (NIH/NEI) [C] Sent: Sunday, June 10, 2007 6:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question? Dear Histonet, Does anyone out there graduated from AFIP knows what is the school code to apply to ASCP? I have a co-worker who needs this and the person who can give her this number is on maternity leave until late august possible September. The person they left in charge is a military who says he doesn't know and told her she has to wait until the person in charge comes back. My coworker doesn't have that much time to apply. Any help would be greatly appreciated. Thanks, Maribel Santiago- Maysonet, HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Mon Jun 11 08:34:33 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Mon Jun 11 07:34:19 2007 Subject: [Histonet] Question? In-Reply-To: Message-ID: Sorry that was not it. Here it is.. DC WASHINGTON ARMED FORCES INST OF PATHOLOGY 908017 HT Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Santiago, Maribel (NIH/NEI) [C] Sent: Sunday, June 10, 2007 6:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question? Dear Histonet, Does anyone out there graduated from AFIP knows what is the school code to apply to ASCP? I have a co-worker who needs this and the person who can give her this number is on maternity leave until late august possible September. The person they left in charge is a military who says he doesn't know and told her she has to wait until the person in charge comes back. My coworker doesn't have that much time to apply. Any help would be greatly appreciated. Thanks, Maribel Santiago- Maysonet, HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From froyer <@t> bitstream.net Mon Jun 11 09:40:00 2007 From: froyer <@t> bitstream.net (Ford Royer) Date: Mon Jun 11 09:40:14 2007 Subject: [Histonet] Bone Saw In-Reply-To: <661949901A768E4F9CC16D8AF8F2838CA1C620@IS-E2K3.grhs.net> References: <964347.28081.qm@web61213.mail.yahoo.com> <661949901A768E4F9CC16D8AF8F2838CA1C620@IS-E2K3.grhs.net> Message-ID: <008c01c7ac36$63f0b7c0$7701a80a@Ford> I agree that the manual saw assembly that Mike points out works just fine and is very economical. I have used a similar home-made device in the past. For power saws, we used a Sears 9? tri-wheel band saw. It was nice and compact so it did not take up much bench space. However it was difficult to clean as it was designed to cut wood and not bone & tissue. Of course in those days, we did not have to worry about many of the pathogens that are present today. A better alternative would be a commercial stainless steel ?butcher?s saw? that could be disinfected, but they were big and clunky and were very cost-prohibitive. In more recent times, there are food quality stainless steel band saws that are designed for easy cleaning and priced more reasonably. Check the link below, or if that does not work go to www.cabelas.com and type in the key word ?band saw?. http://www.cabelas.com/cabelas/en/templates/product/standard-item.jsp?_DARGS =/cabelas/en/common/catalog/item-link.jsp_A&_DAV=search-cat20099&id=00284345 16404a&navCount=2&podId=0028434&parentId=cat20099&masterpathid=&navAction=pu sh&catalogCode=IH&rid=&parentType=index&indexId=cat20099&hasJS=true In regard to considering this type of equipment ?unsafe?, it would depend on how your institution is defining ?unsafe?. If they are concerned that a band saw is unsafe from a bio-hazard stand point, then a stainless steel food-quality instrument would be the answer since it can be easily decontaminated. If they are concerned about operator safety... this can be covered in a proper training protocol and SOPs. With proper training, a motorized band saw is no more dangerous to an operator than a motorized microtome with an exposed blade. ~ Ford Ford M. Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL:? 612-839-1046 Phone:? 763-542-8725 Fax:? 763-546-4830 eMail:? clinicallab@minnesotamedical.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Friday, June 08, 2007 2:18 PM To: Rene J Buesa; Nita Searcy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Bone Saw Try this saw. Works great for any size bone and you can't find one cheaper. http://www.thermo.com/com/cda/product/detail/1,,10121267,00.html Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, June 08, 2007 2:04 PM To: Nita Searcy; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Bone Saw A band saw is the ideal instrument. Just try to "protect (cover)" the saw and use precaution. Also buy the smallest band saw capable of doing the job. I don't think you will find anything better (or cheaper) for large bones. Ren? J. Nita Searcy wrote: I have been asked to obtain a saw for cutting bone. We have the Mar-Med presently but its not "big" enough for larger bones. In the past the institution had a band saw but found it unsafe. Any suggestions? Thanks Nita Searcy Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 254-724-2438 BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division END:VCARD _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- 8:00? 8:25? 8:40? Find a flick in no time with theYahoo! Search movie showtime shortcut. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From santiagom <@t> nei.nih.gov Mon Jun 11 09:52:13 2007 From: santiagom <@t> nei.nih.gov (Santiago, Maribel (NIH/NEI) [C]) Date: Mon Jun 11 09:52:23 2007 Subject: [Histonet] Question? In-Reply-To: <5sshpt$3d6sot@nihcesxway4.hub.nih.gov> References: <5sshpt$3d6sot@nihcesxway4.hub.nih.gov> Message-ID: Thank you so much for the info. I really appreciate it!!! Have a great week!!! Maribel "Minnie" Santiago Maysonet,HTL(ASCP) ? -----Original Message----- From: Douglas D Deltour [mailto:doug@ppspath.com] Sent: Monday, June 11, 2007 9:35 AM To: Santiago, Maribel (NIH/NEI) [C]; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Question? Sorry that was not it. Here it is.. DC WASHINGTON ARMED FORCES INST OF PATHOLOGY 908017 HT Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Santiago, Maribel (NIH/NEI) [C] Sent: Sunday, June 10, 2007 6:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question? Dear Histonet, Does anyone out there graduated from AFIP knows what is the school code to apply to ASCP? I have a co-worker who needs this and the person who can give her this number is on maternity leave until late august possible September. The person they left in charge is a military who says he doesn't know and told her she has to wait until the person in charge comes back. My coworker doesn't have that much time to apply. Any help would be greatly appreciated. Thanks, Maribel Santiago- Maysonet, HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Mon Jun 11 10:23:59 2007 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Mon Jun 11 10:24:14 2007 Subject: [Histonet] Cyclin D1 antibody from Cell Marque user experiences In-Reply-To: <03568ED922E71549B7C3D55569A8C089127B2D@dchq.als.local> Message-ID: Sarah, I use Lab Vision's bcl-1(Cyclin D1) and it looks great! It is an ASR and is a rabbit monoclonal. Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Sarah O'Bryan Sent: Monday, June 11, 2007 1:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cyclin D1 antibody from Cell Marque user experiences Hello all, I would like some information or feedback on how people find different Cyclin D1 antibodies? I can see from some previous messages that it can be a difficult antibody to optimize... I am particularly interested in Cell Marque's users, as we have a customer who would like to know of user experiences with this antibody before she decides whether to trial or not. If you use Cyclin D1 in your laboratory, would you please let me know what clone you use, who manufactures it, and what you think of it? What is the leading antibody on the market? Thanks for your help. Sarah _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rebecca.jones256 <@t> gmail.com Mon Jun 11 10:40:56 2007 From: rebecca.jones256 <@t> gmail.com (Rebecca Jones) Date: Mon Jun 11 10:41:07 2007 Subject: [Histonet] Xylene Substitute Message-ID: I know this topic has been on here before, but of course I paid little attention as it wasn't on top of my busy list! And I rarely converse over the histonet, but definately watch as the conversations move along. I work in a small research lab, but recently got a call from CBG Biotech to try their xylene substitute, Formula 83 for free. I have only ever used xylene, but am fairly new to histologic procecures so I am considering an alternative that is less toxic. Has anyone tried Formula 83 or have any other substitutes they can suggest. I will try my hardest to post the results of my testing so that everyone can know. I think once I get the sample in I can test things fairly quickly. I would like to sample more than one though, just to have a comparisson. Thanks, Rebecca Jones From jessgrocki <@t> yahoo.com Mon Jun 11 10:59:14 2007 From: jessgrocki <@t> yahoo.com (Jessica Piche) Date: Mon Jun 11 10:59:46 2007 Subject: [Histonet] Paraffin Block Storage Message-ID: <388238.47610.qm@web82002.mail.mud.yahoo.com> Hello! Just wanted to say thanks for all the great ideas for block storage!! Jessica From lcastillos <@t> cogenics.com Mon Jun 11 11:01:56 2007 From: lcastillos <@t> cogenics.com (Castillos, Luminita) Date: Mon Jun 11 11:02:08 2007 Subject: [Histonet] glycerol-coated glass slides Message-ID: Hi everybody, I am looking to find a protocol for coating charge-free glass slides with 3% glycerol. Does anybody has a protocol of this type or maybe knows a vendor who delivers ready-to-use glycerol-coated glass slides?. Thank you for any feedback on this issue. Sincerely, L From rjbuesa <@t> yahoo.com Mon Jun 11 11:36:31 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jun 11 11:36:35 2007 Subject: [Histonet] glycerol-coated glass slides In-Reply-To: Message-ID: <796511.54390.qm@web61217.mail.yahoo.com> Luminita: Frankly I am at a loss with your question. Glycerol (propano-triol) is an alcohol and will not stick to the glass, even less at a 3% concentration. Glycerol sometimes is used as a component for some mounting fluids and in those cases it has to be contained within a solid ring (of sealant) to prevent its evaporation. Do you have a specific technique you need the glycerol for? Could you be more specific? Ren? J. "Castillos, Luminita" wrote: Hi everybody, I am looking to find a protocol for coating charge-free glass slides with 3% glycerol. Does anybody has a protocol of this type or maybe knows a vendor who delivers ready-to-use glycerol-coated glass slides?. Thank you for any feedback on this issue. Sincerely, L _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Building a website is a piece of cake. Yahoo! Small Business gives you all the tools to get online. From jcline <@t> wchsys.org Mon Jun 11 11:43:47 2007 From: jcline <@t> wchsys.org (Joyce Cline) Date: Mon Jun 11 11:43:53 2007 Subject: [Histonet] Xylene Substitute, Rebecca In-Reply-To: Message-ID: <001901c7ac47$aeeb7330$1d2a14ac@wchsys.org> I am using Formula 83 and the recycler. Coverslipping with Formula 83 is good and the slides can be filed in 8-10 days. Formula 83 recycles really well also. Formula 83 is more comparable to Xylene then any other Xylene substitute that I have used. I had used Clearite 3 for over 15 years. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rebecca Jones Sent: Monday, June 11, 2007 11:41 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene Substitute I know this topic has been on here before, but of course I paid little attention as it wasn't on top of my busy list! And I rarely converse over the histonet, but definately watch as the conversations move along. I work in a small research lab, but recently got a call from CBG Biotech to try their xylene substitute, Formula 83 for free. I have only ever used xylene, but am fairly new to histologic procecures so I am considering an alternative that is less toxic. Has anyone tried Formula 83 or have any other substitutes they can suggest. I will try my hardest to post the results of my testing so that everyone can know. I think once I get the sample in I can test things fairly quickly. I would like to sample more than one though, just to have a comparisson. Thanks, Rebecca Jones _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From gu.lang <@t> gmx.at Mon Jun 11 11:50:56 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Mon Jun 11 11:51:03 2007 Subject: [Histonet] isolated nuclei suspension for FISH Message-ID: <000001c7ac48$aeda8dd0$6412a8c0@dielangs.at> A question for those who deal with isolated nuclei preparation for FISH. We struggle around with our protocol. We take 2-3 30 ?m slides, deparaffinize them in an Eppendorf cup with Xylol, then ethanol abs., then 2xSSC at 75 degree. Afterwards follows the digestion (Pepsin 4 mg/ml in 0,9% NaCl pH 1,5) for 25 min, 37 degree waterbath. Then the hopefully harvested nuclei are washed in 3 times PBS, given in 0,075 M KCl, washed again and fixed in icecold aceticacid/Methanol. Today we worked 5 hours on the preparation and at the end there was a tiny, clear 1 ml solution. My colleage and me were "a little bit" disappointed -hmm. How should the solution look after the digestion? Are the slides totally solved or can they still be seen? What is the parameter for the correct incubation-time? Is it very important to use a centrifuge without break? Is the end-result turbid or clear? What slides are to be used for spreading the nuclei: Superfrost Plus or untreated glass slides? Wet or dry? I am glad about any hint! Gudrun Lang Histologie Akh Linz, Austria From lcastillos <@t> cogenics.com Mon Jun 11 11:51:08 2007 From: lcastillos <@t> cogenics.com (Castillos, Luminita) Date: Mon Jun 11 11:51:16 2007 Subject: [Histonet] glycerol-coated glass slides In-Reply-To: <796511.54390.qm@web61217.mail.yahoo.com> Message-ID: Hi Rene, It's a very good point. I am following a method found in a publication which is focused on the efficiency of microdissection of skin punch biopsies. The strong intercellular adhesive forces from the skin reduce the adhesive forces of laser film to the tissue and consequently the preparation of the samples for LCM. For the successful lift of cells it is critical that the force of attraction between the tissue and film be greater than that of the tissue to the slide. Electrostatic forces are minimized by the coating of the glass slides prior to cryosection with 3% glycerol and utilizing high-quality charge-free Superfrost slides. Based on this, I was wondering if there is a specific protocol of glycerol-coating slides to help with microdissection. Thanks Rene. L, ________________________________ From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Monday, June 11, 2007 12:37 PM To: Castillos, Luminita; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] glycerol-coated glass slides Luminita: Frankly I am at a loss with your question. Glycerol (propano-triol) is an alcohol and will not stick to the glass, even less at a 3% concentration. Glycerol sometimes is used as a component for some mounting fluids and in those cases it has to be contained within a solid ring (of sealant) to prevent its evaporation. Do you have a specific technique you need the glycerol for? Could you be more specific? Ren? J. "Castillos, Luminita" wrote: Hi everybody, I am looking to find a protocol for coating charge-free glass slides with 3% glycerol. Does anybody has a protocol of this type or maybe knows a vendor who delivers ready-to-use glycerol-coated glass slides?. Thank you for any feedback on this issue. Sincerely, L _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Building a website is a piece of cake. Yahoo! Small Business gives you all the tools to get online. From Charlotte.Kopczynski <@t> baycare.org Mon Jun 11 12:18:34 2007 From: Charlotte.Kopczynski <@t> baycare.org (Kopczynski, Charlotte) Date: Mon Jun 11 12:18:39 2007 Subject: [Histonet] Question about Coverslippers & Barcoded Slides Message-ID: Can anyone out there give me some feedback on auto-coverslippers and Ventana bar-coded slides. We have found that our Leica coverslipper will not work on slides that have a barcode label (example Ventana Immuno stained slides) We thought that the TBS coverslipper would work and did on dry slides with barcode labels. However, after purchasing a TBS coverslipper, we found that after the slides had been dehydrated and cleared in xylene, there is some glue from the barcode label which oozes out the sides of the label and the slides get stuck in the coverslipper. Has anyone else found a coverslipper that works or any recommendations. We are immunostaining 300+ slides per day and manual coverslipping is a problem. Thanks, Charlotte Kopczynski,HTL(ASCP) Regional Pathology Manager BayCare Laboratories Phone: 727-461-8246 Fax: 727-462-7596 No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. Confidential: This electronic message and all contents contain information from BayCare Health System which may be privileged, confidential or otherwise protected from disclosure. The information is intended to be for the addressee only. If you are not the addressee, any disclosure, copy, distribution or use of the contents of this message is prohibited. If you have received this electronic message in error, please notify the sender and destroy the original message and all copies. From settembr <@t> umdnj.edu Mon Jun 11 12:38:21 2007 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Mon Jun 11 12:39:10 2007 Subject: [Histonet] Cyclin D1 antibody from Cell Marque user experiences Message-ID: Sarah, I use the same Lab Vision Cyclin D1, rabbit monoclonal as mentioned below. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> "Dawson, Glen" 06/11/07 11:23 AM >>> Sarah, I use Lab Vision's bcl-1(Cyclin D1) and it looks great! It is an ASR and is a rabbit monoclonal. Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Sarah O'Bryan Sent: Monday, June 11, 2007 1:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cyclin D1 antibody from Cell Marque user experiences Hello all, I would like some information or feedback on how people find different Cyclin D1 antibodies? I can see from some previous messages that it can be a difficult antibody to optimize... I am particularly interested in Cell Marque's users, as we have a customer who would like to know of user experiences with this antibody before she decides whether to trial or not. If you use Cyclin D1 in your laboratory, would you please let me know what clone you use, who manufactures it, and what you think of it? What is the leading antibody on the market? Thanks for your help. Sarah _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Mon Jun 11 12:41:05 2007 From: tkngflght <@t> yahoo.com (Cheryl R. Kerry) Date: Mon Jun 11 12:40:44 2007 Subject: [Histonet] 'Tis the season--a huge number of permanent openings In-Reply-To: Message-ID: <004d01c7ac4f$b062b040$6801a8c0@CHERYLSLAPTOP> Hi All! Summer is the season for change! We have an unprecedented number of permanent job openings! From routine bench through full non-bench supervisor and a few managerial positions to keep things interesting. They are all over the country, in all sorts of labs and on all shifts. As a working histotech I know that not all jobs fit all techs. Rather than post a laundry list I ask that you invest five minutes on the phone with me--share your wish list, likes and dislikes and your career aspirations (I LOVE these conversations!!). If we don't have a job you want, we'll go to work to FIND IT FOR YOU!! It's called 'the agent approach' and it's like having your own job search specialist on staff without having to spend hours each day looking for your next career move. Our services are free and in addition we help with: --Resume support and writing (don't panic if you don't have one--creating one can be FUN!) --Coaching: both pre-phone interview and pre-face-to-face with post interview debriefing. --Information on the lab itself: we'll tell you the truth and if we don't know, we'll do our best to find out. --All travel expenses for interviews paid. --Sponsoring temp-to-perm where applicable --Compensation & offer negotiation (support and coaching or we can handle it for you to help you get the best possible package) --Knowledgeable and experienced histology staff--you won't have to explain what you do or wonder if we 'get it'. We talk the same language: we have the same goal. Five minutes and you'll be on your way to a place where you walk in happy, and happy to be there!! Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. 800.756.3309 fax and phone 281.852.9457 phone admin@fullstaff.org If you're looking for something different--ask about our travel tech opportunities! From burch007 <@t> mc.duke.edu Mon Jun 11 12:41:06 2007 From: burch007 <@t> mc.duke.edu (James L Burchette) Date: Mon Jun 11 12:41:17 2007 Subject: [Histonet] P53 survey In-Reply-To: <000001c7ac48$aeda8dd0$6412a8c0@dielangs.at> Message-ID: Dear Histonetter's I'm curious, what p53 clone do you use and what is your preferred HIER pretreatment on FFPE human tissue? Sincerely, Jim Burchette, HT(ASCP) QIHC "A simple histotech from a little country hospital in North Carolina" From burch007 <@t> mc.duke.edu Mon Jun 11 12:41:06 2007 From: burch007 <@t> mc.duke.edu (James L Burchette) Date: Mon Jun 11 12:45:11 2007 Subject: [Histonet] P53 survey In-Reply-To: <000001c7ac48$aeda8dd0$6412a8c0@dielangs.at> Message-ID: Dear Histonetter's I'm curious, what p53 clone do you use and what is your preferred HIER pretreatment on FFPE human tissue? Sincerely, Jim Burchette, HT(ASCP) QIHC "A simple histotech from a little country hospital in North Carolina" From gu.lang <@t> gmx.at Mon Jun 11 12:48:18 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Mon Jun 11 12:48:25 2007 Subject: AW: [Histonet] Question about Coverslippers & Barcoded Slides In-Reply-To: Message-ID: <000f01c7ac50$b225eea0$6412a8c0@dielangs.at> Our experience is, that if the slides with barcode label stay too long in Xylol or similar reagens, the glue will come out and stick on the edges. We solved the problem by decreasing the reagens-level in the jar and the time. We have a Leica-coverslipper. After a definite period of time, we clean the clamps with Butylacetat. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Kopczynski, Charlotte Gesendet: Montag, 11. Juni 2007 19:19 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Question about Coverslippers & Barcoded Slides Can anyone out there give me some feedback on auto-coverslippers and Ventana bar-coded slides. We have found that our Leica coverslipper will not work on slides that have a barcode label (example Ventana Immuno stained slides) We thought that the TBS coverslipper would work and did on dry slides with barcode labels. However, after purchasing a TBS coverslipper, we found that after the slides had been dehydrated and cleared in xylene, there is some glue from the barcode label which oozes out the sides of the label and the slides get stuck in the coverslipper. Has anyone else found a coverslipper that works or any recommendations. We are immunostaining 300+ slides per day and manual coverslipping is a problem. Thanks, Charlotte Kopczynski,HTL(ASCP) Regional Pathology Manager BayCare Laboratories Phone: 727-461-8246 Fax: 727-462-7596 No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. Confidential: This electronic message and all contents contain information >from BayCare Health System which may be privileged, confidential or otherwise protected from disclosure. The information is intended to be for the addressee only. If you are not the addressee, any disclosure, copy, distribution or use of the contents of this message is prohibited. If you have received this electronic message in error, please notify the sender and destroy the original message and all copies. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From contact <@t> excaliburpathology.com Mon Jun 11 12:14:19 2007 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Mon Jun 11 12:59:15 2007 Subject: [Histonet] glycerol-coated glass slides Message-ID: <101285.21551.qm@web50101.mail.re2.yahoo.com> Nearly 30 years ago I used glycerol mixed with albumin to coat glass slides for eye secctions. This is probably what you are referring to. I have not used this method for about 25 years though. I found chrome alum-gelatin worked much better and never looked back. With albumin-glycerol you can get eosinophilic staining on the slide if the coating is too thick. Paula Pierce, BS, HTL(ASCP)HT Excalibur Pathology, Inc. 630 N. Broadway Moore, OK 73160 405-759-3953 contact@excaliburpathology.com www.excaliburpathology.com From Eric.C.Kellar <@t> questdiagnostics.com Mon Jun 11 12:59:56 2007 From: Eric.C.Kellar <@t> questdiagnostics.com (Kellar, Eric C) Date: Mon Jun 11 13:00:16 2007 Subject: [Histonet] Question about Coverslippers & Barcoded Slides Message-ID: <6843061CE6B98E4B96590D4F299618F801583C2B@qdcws0117.us.qdx.com> Charlotte, I have success with the Sakura Tissue-Tek film coverslipper set on "short" coverslip with the Ventana bar code labels. The glue does deposit on the chain drive, but daily cleaning will avoid, 'gunking up the works'. You may be able to purchase a used one. It's better than coverslipping 300+ slides by hand or standing guard with your finger on "STOP" button over the glass coverslipper. Reduction of the clearing agent levels prior to coverslipping helps too. Eric C. Kellar Quest Diagnostics Histology Laboratory Supervisor South Florida -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kopczynski, Charlotte Sent: Monday, June 11, 2007 1:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question about Coverslippers & Barcoded Slides Can anyone out there give me some feedback on auto-coverslippers and Ventana bar-coded slides. We have found that our Leica coverslipper will not work on slides that have a barcode label (example Ventana Immuno stained slides) We thought that the TBS coverslipper would work and did on dry slides with barcode labels. However, after purchasing a TBS coverslipper, we found that after the slides had been dehydrated and cleared in xylene, there is some glue from the barcode label which oozes out the sides of the label and the slides get stuck in the coverslipper. Has anyone else found a coverslipper that works or any recommendations. We are immunostaining 300+ slides per day and manual coverslipping is a problem. Thanks, Charlotte Kopczynski,HTL(ASCP) Regional Pathology Manager BayCare Laboratories Phone: 727-461-8246 Fax: 727-462-7596 No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. Confidential: This electronic message and all contents contain information from BayCare Health System which may be privileged, confidential or otherwise protected from disclosure. The information is intended to be for the addressee only. If you are not the addressee, any disclosure, copy, distribution or use of the contents of this message is prohibited. If you have received this electronic message in error, please notify the sender and destroy the original message and all copies. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ============================================================================== The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. ============================================================================== From GDawson <@t> dynacaremilwaukee.com Mon Jun 11 13:06:56 2007 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Mon Jun 11 13:07:03 2007 Subject: [Histonet] P53 survey In-Reply-To: Message-ID: Jim, I use Dako's monoclonal p53 (clone DO-7) with Dako's pH 6.0 citrate target retrieval for my HIER. It works very well. Good Luck, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of James L Burchette Sent: Monday, June 11, 2007 12:41 PM To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] P53 survey Dear Histonetter's I'm curious, what p53 clone do you use and what is your preferred HIER pretreatment on FFPE human tissue? Sincerely, Jim Burchette, HT(ASCP) QIHC "A simple histotech from a little country hospital in North Carolina" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From settembr <@t> umdnj.edu Mon Jun 11 13:16:46 2007 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Mon Jun 11 13:17:39 2007 Subject: [Histonet] P53 survey Message-ID: Hello Jim I use Dako's p53 clone DO-7 as well with Dako's Target Retrieval Solutin in a steamer. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> "Dawson, Glen" 06/11/07 2:06 PM >>> Jim, I use Dako's monoclonal p53 (clone DO-7) with Dako's pH 6.0 citrate target retrieval for my HIER. It works very well. Good Luck, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of James L Burchette Sent: Monday, June 11, 2007 12:41 PM To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] P53 survey Dear Histonetter's I'm curious, what p53 clone do you use and what is your preferred HIER pretreatment on FFPE human tissue? Sincerely, Jim Burchette, HT(ASCP) QIHC "A simple histotech from a little country hospital in North Carolina" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From awatanabe <@t> tgen.org Mon Jun 11 13:28:03 2007 From: awatanabe <@t> tgen.org (Aprill Watanabe) Date: Mon Jun 11 13:28:09 2007 Subject: [Histonet] Re: Xylene Substitute In-Reply-To: <20070611170223.5935B2005812@mr1.tgen.org> Message-ID: I also work in research and routinely do H&E's and lots of IHC. I tried Biocare's Slide Brite and found it difficult to use as it requires a specific mounting media. It was also a little greasy to the touch. I then tried StatLab's XS-3. This one I found was severely subject to water in the reagent. If the slides were not cleared well it left water on the slide. Again you have to have the right mounting media which they sell as well. I found this substitute to bleed out some of my IHC DAB staining. It also took at least a day to a day and half to dry. My last one and the one I'm currently using is Pro-Par from Anatech. It also requires their mounting media but the reagents are at a fair price. I do not do any processing so I have tested any of these on a processor but they all worked for H&E and IHC aspects. The Pro-oar I have found is the closest to Xylene that I have tested. It's not limonene based so it's not greasy at all and it dries in a matter of hours not days. Again clearing well in alcohol is critical. I would recommend this one to anyone looking for an alternative. They also sell a dehydrant. This is just my two cents. Aprill Watanabe, B.S. Research Associate Tissue Microarray Center (TMA) Translational Genomics Research Institute (TGen) 602-343-8822 awatanabe@tgen.org www.tgen.org From LSebree <@t> uwhealth.org Mon Jun 11 13:34:11 2007 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Mon Jun 11 13:34:18 2007 Subject: [Histonet] P53 survey In-Reply-To: Message-ID: Jim, We use Biocare's p53 at 1:50 with Biocare's Bull's Eye, Diva or BORG for 2" in their Decloaking chamber (pressure cooker). Or we use Ventana's standard CC1 HIER on our VMS BenchMark and XT. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James L Burchette Sent: Monday, June 11, 2007 12:41 PM To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] P53 survey Dear Histonetter's I'm curious, what p53 clone do you use and what is your preferred HIER pretreatment on FFPE human tissue? Sincerely, Jim Burchette, HT(ASCP) QIHC "A simple histotech from a little country hospital in North Carolina" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From smehta <@t> magenbiosciences.com Mon Jun 11 13:48:04 2007 From: smehta <@t> magenbiosciences.com (Shanu Mehta) Date: Mon Jun 11 13:48:09 2007 Subject: [Histonet] Leica ASP300S carbon filter Message-ID: <4C78CF3C61E6A640888226885709EFE908C290@server01.magen.local> Hello all, We have a Leica ASP300s tissue processor. My question is regarding the activated carbon filter. What is the best way to dispose off the used filer? Also, what is the best way to order a new filter? I just changed my last filter and will need a new one in the next few months. Is it available from any other supplier or does Leica have a monopoly on them? Thanks, Shanu ------------ Shanu Mehta Magen Biosciences 790 Memorial Drive Suite 101 Cambridge, MA 02139 Phone: (617) 494-8732 x 2212 Fax: (617) 494-8752 Email: smehta@magenbiosciences.com http://www.magenbiosciences.com From gcallis <@t> montana.edu Mon Jun 11 14:06:49 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Jun 11 14:06:24 2007 Subject: Propar Re: [Histonet] Re: Xylene Substitute In-Reply-To: References: <20070611170223.5935B2005812@mr1.tgen.org> Message-ID: <6.0.0.22.1.20070611124216.01b39c30@gemini.msu.montana.edu> Propar is a single aliphatic hydrocarbon, derived from petroleum but I think the MSDS gives this information. It is very similar to Clearite 3 (Richard Allan). However, both are still sensitive to water so good dehydration is critical prior to clearing by these two solvents for both staining and processing. We have used both for processing and staining, but pay attention to solvent rotation in order to maintain freshness and no water carryover into these clearing agents changes. At 12:28 PM 6/11/2007, you wrote: >I also work in research and routinely do H&E's and lots of IHC. I tried >Biocare's Slide Brite and found it difficult to use as it requires a >specific mounting media. It was also a little greasy to the touch. I then >tried StatLab's XS-3. This one I found was severely subject to water in the >reagent. If the slides were not cleared well it left water on the slide. >Again you have to have the right mounting media which they sell as well. I >found this substitute to bleed out some of my IHC DAB staining. It also >took at least a day to a day and half to dry. My last one and the one I'm >currently using is Pro-Par from Anatech. It also requires their mounting >media but the reagents are at a fair price. I do not do any processing so I >have tested any of these on a processor but they all worked for H&E and IHC >aspects. The Pro-oar I have found is the closest to Xylene that I have >tested. It's not limonene based so it's not greasy at all and it dries in a >matter of hours not days. Again clearing well in alcohol is critical. I >would recommend this one to anyone looking for an alternative. They also >sell a dehydrant. This is just my two cents. > > > >Aprill Watanabe, B.S. >Research Associate >Tissue Microarray Center (TMA) >Translational Genomics Research Institute (TGen) >602-343-8822 >awatanabe@tgen.org >www.tgen.org > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From Maxim_71 <@t> mail.ru Mon Jun 11 14:12:40 2007 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Mon Jun 11 14:13:33 2007 Subject: [Histonet] Xylene Substitute Message-ID: <538496715.20070611231240@mail.ru> Rebecca: Earlier I used xylene, chloroform and mixture of aliphatics hydrocarbons (C6-C8). All these reagents gave to me good results for years. As dehydratant I used ethanol and acetone. Isopropanol and mineral oil are very gentle for tissues and lowest toxic for personnel. These chemicals is very best for our lab. We processes all our specimens manually. Each year we have approximately 70-80K blocks. Sorry, but all other commercial reagents not available for us... Sincerely, Maxim Peshkov Russia Taganrog. From mari.ann.mailhiot <@t> leica-microsystems.com Mon Jun 11 14:52:29 2007 From: mari.ann.mailhiot <@t> leica-microsystems.com (mari.ann.mailhiot@leica-microsystems.com) Date: Mon Jun 11 14:52:31 2007 Subject: [Histonet] Leica ASP300S carbon filter In-Reply-To: <4C78CF3C61E6A640888226885709EFE908C290@server01.magen.local> Message-ID: Shanu You can dispose of the filter in the regular garbage. The carbon in the filter neutralizes everything that passes through it. You will have to order your carbon filter directly from Leica. Best Regards Mari Ann Mailhiot BA HT ASCP Application Specialist Leica Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com "Shanu Mehta" To Sent by: histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] Leica ASP300S carbon filter 06/11/2007 01:48 PM Hello all, We have a Leica ASP300s tissue processor. My question is regarding the activated carbon filter. What is the best way to dispose off the used filer? Also, what is the best way to order a new filter? I just changed my last filter and will need a new one in the next few months. Is it available from any other supplier or does Leica have a monopoly on them? Thanks, Shanu ------------ Shanu Mehta Magen Biosciences 790 Memorial Drive Suite 101 Cambridge, MA 02139 Phone: (617) 494-8732 x 2212 Fax: (617) 494-8752 Email: smehta@magenbiosciences.com http://www.magenbiosciences.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From sbanwait <@t> buckinstitute.org Mon Jun 11 15:36:15 2007 From: sbanwait <@t> buckinstitute.org (Surita Banwait) Date: Mon Jun 11 15:36:26 2007 Subject: [Histonet] mouse heart, diaphragm, quadriceps, soleus and psoas morphology Message-ID: Hi There, I was wondering if someone has H and E (preferably on paraffin processed) images (labeled) for these regions (mouse heart ,diaphragm, quadriceps, soleus and psoas) or if you can pass along some references that I could check out. I've tried doing an internet search with no avail. Thanks, Surita ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Surita Banwait Morphology & Imaging Core Research Associate II Buck Institute for Age Research 8001 Redwood Blvd. Novato, CA 94945 415-209-2221 sbanwait@buckinstitute.org ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ From rjbuesa <@t> yahoo.com Mon Jun 11 15:54:14 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jun 11 15:54:26 2007 Subject: [Histonet] P53 survey In-Reply-To: Message-ID: <45843.85440.qm@web61215.mail.yahoo.com> Clone from DAKO, diluted 1:80 wit pH6 HIER Ren? J. James L Burchette wrote: Dear Histonetter's I'm curious, what p53 clone do you use and what is your preferred HIER pretreatment on FFPE human tissue? Sincerely, Jim Burchette, HT(ASCP) QIHC "A simple histotech from a little country hospital in North Carolina" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Park yourself in front of a world of choices in alternative vehicles. Visit the Yahoo! Auto Green Center. From ElizabethWyand <@t> texashealth.org Mon Jun 11 16:00:55 2007 From: ElizabethWyand <@t> texashealth.org (Wyand, Elizabeth) Date: Mon Jun 11 15:59:29 2007 Subject: [Histonet] Histology position available In-Reply-To: <002801c7a392$45bd1200$6701a8c0@Histopath.net> References: <002801c7a392$45bd1200$6701a8c0@Histopath.net> Message-ID: <26BE9ACC202D29479B0A144066A733E50276D7C3@phdex01.txhealth.org> Our private histology lab is accepting applications for a certified Histotechnician for our Plano location. Excellent salary and benefits. Shift hours variable but moving to 4 x 10s in late July. Fax your resume to: 972.981.3236 ________________________________________________ Elizabeth Wyand Administrator MD Pathology 6124 West Parker Road, Suite G36 Plano, Texas 75093 972.981.3107 972.981.3236 (fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. From Greg.Bowlay <@t> mater.org.au Tue Jun 12 01:36:01 2007 From: Greg.Bowlay <@t> mater.org.au (Bowlay, Greg) Date: Tue Jun 12 01:36:17 2007 Subject: [Histonet] Cyclin D1 antibody from Cell Marque user experiences References: <03568ED922E71549B7C3D55569A8C089127B2D@dchq.als.local> Message-ID: <499E3F06E3FDA043801D7ACB71B055220174B891@matexch1.mater.org.au> Hi Sarah, We use Cyclin D1 SP4 from Neomarkers, like young Barry, but we use a dilution of 1:200 on Ventana Benchmark requiring only mild CC1 (on-board retrieval), primary incubn of 32 minutes using the iView detection system. regards, Greg Bowlay Scientist Anatomical Pathology Mater Health Services South Brisbane Q 4101 Greg.Bowlay@mater.org.au PH: +61 7 3840 8586 FAX: +61 7 3840 8338 "Far better it is to dare mighty things to win glorious triumphs even though chequered by failure, than to rank with those poor spirits who neither enjoy nor suffer much because they live in the grey twilight that knows neither victory nor defeat." Theodore Roosevelt ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Sarah O'Bryan Sent: Mon 11/6/07 4:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cyclin D1 antibody from Cell Marque user experiences Hello all, I would like some information or feedback on how people find different Cyclin D1 antibodies? I can see from some previous messages that it can be a difficult antibody to optimize... I am particularly interested in Cell Marque's users, as we have a customer who would like to know of user experiences with this antibody before she decides whether to trial or not. If you use Cyclin D1 in your laboratory, would you please let me know what clone you use, who manufactures it, and what you think of it? What is the leading antibody on the market? Thanks for your help. Sarah _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, together with any attachments, is confidential and intended for the named recipient(s) only. If you are not the intended recipient or have received this message in error, you are asked to immediately notify the sender and delete this message and any copies of this message from your computer system network and destroy any printed copies of this email. Any form of unauthorised disclosure, modification, distribution, publication or use of this e-mail message is prohibited. From Jacqueline.Malam <@t> rli.mbht.nhs.uk Tue Jun 12 02:38:02 2007 From: Jacqueline.Malam <@t> rli.mbht.nhs.uk (Malam Jacqueline) Date: Tue Jun 12 02:41:33 2007 Subject: [Histonet] Re: Cyclin D1 Message-ID: We use Lab Vision's SP4 rabbit monoclonal on the Ventana Benchmark XT with standard CC1 heat retrieval and room temperature incubation for 56 minutes at a dilution of 1/100. Excellent results. Jacqui malam Lancaster uk DISCLAIMER: This e-mail is confidential and privileged. If you are not the intended recipient please accept our apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform postmaster@rli.mbht.nhs.uk that this message has gone astray before deleting it. Comments or opinions expressed in this email are those of their respective contributors only. The views expressed do not represent the views of the Trust, its management or employees. University Hospitals of Morecambe Bay NHS Trust is not responsible and disclaims any and all liability for the content of comments written within.Thank you for your co-operation. From nuno_apct <@t> portugalmail.pt Tue Jun 12 04:34:01 2007 From: nuno_apct <@t> portugalmail.pt (nuno_apct@portugalmail.pt) Date: Tue Jun 12 04:34:07 2007 Subject: [Histonet] gram + and brain In-Reply-To: <20070611170718.17FB3339DA@elfhild.portugalmail.pt> References: <20070611170718.17FB3339DA@elfhild.portugalmail.pt> Message-ID: <1181640841.466e688948033@webmail4.portugalmail.pt> I have a co-worker that would to do a Gram counterstaing on brain tissue. The bacteria is agalactium, a Gram + one. The problem is, there are no protocols specific for that. Pleaseeeeeee help :) __________________________________________________________ O email preferido dos portugueses agora com 2 000 MB de espa?o e acesso gratuito ? Internet http://www.portugalmail.pt/2000mb From BMolinari <@t> heart.thi.tmc.edu Tue Jun 12 05:44:04 2007 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Tue Jun 12 05:44:06 2007 Subject: [Histonet] Xylene Substitute Message-ID: Hi Rebecca, I changed from Xylene to Formula 83 for my stainer/coverslipper and I have had no problems. Definitely less fumes! Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rebecca Jones Sent: Monday, June 11, 2007 10:41 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene Substitute I know this topic has been on here before, but of course I paid little attention as it wasn't on top of my busy list! And I rarely converse over the histonet, but definately watch as the conversations move along. I work in a small research lab, but recently got a call from CBG Biotech to try their xylene substitute, Formula 83 for free. I have only ever used xylene, but am fairly new to histologic procecures so I am considering an alternative that is less toxic. Has anyone tried Formula 83 or have any other substitutes they can suggest. I will try my hardest to post the results of my testing so that everyone can know. I think once I get the sample in I can test things fairly quickly. I would like to sample more than one though, just to have a comparisson. Thanks, Rebecca Jones _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rebecca.jones256 <@t> gmail.com Tue Jun 12 08:19:01 2007 From: rebecca.jones256 <@t> gmail.com (Rebecca Jones) Date: Tue Jun 12 08:19:08 2007 Subject: [Histonet] Xylene Substitute In-Reply-To: References: Message-ID: You have all been very helpful. I am going to be trying Formula 83?, by CBG Biotech and Clear Rite 3 by Richard Allan. I had many great suggestions, but only want to do two evaluations, as like many of you I'm so busy with routine work! The samples are on the way and I will post my findings, once I get to the testing. Thanks again, Rebecca Jones On 6/12/07, Molinari, Betsy wrote: > > Hi Rebecca, > I changed from Xylene to Formula 83 for my stainer/coverslipper and I > have had no problems. Definitely less fumes! > > Betsy Molinari HT (ASCP) > Texas Heart Institute > Cardiovascular Pathology > 6770 Bertner Ave. > Houston,TX 77030 > 832-355-6524 > 832-355-6812 (fax) > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rebecca > Jones > Sent: Monday, June 11, 2007 10:41 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Xylene Substitute > > I know this topic has been on here before, but of course I paid little > attention as it wasn't on top of my busy list! And I rarely converse > over > the histonet, but definately watch as the conversations move along. > > I work in a small research lab, but recently got a call from CBG Biotech > to > try their xylene substitute, Formula 83 for free. > > I have only ever used xylene, but am fairly new to histologic procecures > so > I am considering an alternative that is less toxic. > > Has anyone tried Formula 83 or have any other substitutes they can > suggest. > > > I will try my hardest to post the results of my testing so that everyone > can > know. I think once I get the sample in I can test things fairly > quickly. I > would like to sample more than one though, just to have a comparisson. > > Thanks, > > Rebecca Jones > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From relia1 <@t> earthlink.net Tue Jun 12 08:20:22 2007 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Jun 12 08:20:26 2007 Subject: [Histonet] Travelers, Temp Techs, Travel Techs - Histology Message-ID: New Places to see, New people to meet, New opportunities Tired of packing and unpacking; Lonely for folks at home; Not sure where your next assignment might be? Do you want to settle down back home; is there someplace you?ve always wanted to live or is there a place where you?ve temped that you?d like to settle down in? Tired of the Grind? I can help!! My name is Pam Barker and my Company is RELIA. We are the only recruiting firm in the nation working EXCLUSIVELY in nationwide permanent placement of histology professionals. I offer over 25 years of experience in assisting people with making job changes and career transitions and over 5 years working exclusively with histology professionals. Do you want to return to permanent work? Today, tomorrow, 6 months from now, a year from now. I am an expert at transitioning histo techs from temporary to permanent positions. I provide resume assistance, and personal support and coaching from the interview through the entire process and remain available to you once you start your new dream job. Send me an e-mail at relia1@earthlink.net or give me a call at 866-607-3542. Let?s talk about what would appeal to you in a permanent position. If you would like I could send you my career bulletins listing my latest positions. My services are free of charge to you. All fees are paid by my client companies. Remember it never hurts to look!! Thanks-Pam 866-607-3542. Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net From rjbuesa <@t> yahoo.com Tue Jun 12 09:01:06 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jun 12 09:01:11 2007 Subject: [Histonet] gram + and brain In-Reply-To: <1181640841.466e688948033@webmail4.portugalmail.pt> Message-ID: <934584.41705.qm@web61219.mail.yahoo.com> You can find in any book dealing with histotechnique the "Brown-Brenn" procedure for Gram staining of tissue. It is essentially the same Gram procedure, but with the dewaxing and clearing steps. Rem? J. nuno_apct@portugalmail.pt wrote: I have a co-worker that would to do a Gram counterstaing on brain tissue. The bacteria is agalactium, a Gram + one. The problem is, there are no protocols specific for that. Pleaseeeeeee help :) __________________________________________________________ O email preferido dos portugueses agora com 2 000 MB de espa?o e acesso gratuito ? Internet http://www.portugalmail.pt/2000mb _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Fussy? Opinionated? Impossible to please? Perfect. Join Yahoo!'s user panel and lay it on us. From vd38 <@t> georgetown.edu Tue Jun 12 11:29:08 2007 From: vd38 <@t> georgetown.edu (Vernon Dailey) Date: Tue Jun 12 11:29:21 2007 Subject: [Histonet] Brain Pigment Message-ID: <466EC9D4.5080604@georgetown.edu> Hi Histonet, I am trying IHC on some human brain tissue. The region of interest is the substantia nigra. The pigment in the SN cells is interfering with my IHC. I have a few protocols for removing this pigment but they are very damaging to the sections. Does anyone have a protocol that works well for this purpose? Any help would be appreciated. Best, Vernon From liz <@t> premierlab.com Tue Jun 12 11:39:51 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Tue Jun 12 11:40:09 2007 Subject: [Histonet] Brain Pigment In-Reply-To: <466EC9D4.5080604@georgetown.edu> Message-ID: <000001c7ad10$4d0fc6b0$6d00a8c0@PremierLab.local> Vernon I'm assuming that your pigment is brown, if that's the case then I would switch to an alkaline phosphatase detection system with a red chromagen such as fast red. If that's not then there are other colored chromagens that could be used. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vernon Dailey Sent: Tuesday, June 12, 2007 10:29 AM To: Histonet Subject: [Histonet] Brain Pigment Hi Histonet, I am trying IHC on some human brain tissue. The region of interest is the substantia nigra. The pigment in the SN cells is interfering with my IHC. I have a few protocols for removing this pigment but they are very damaging to the sections. Does anyone have a protocol that works well for this purpose? Any help would be appreciated. Best, Vernon _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Jun 12 11:42:31 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jun 12 11:42:36 2007 Subject: [Histonet] Brain Pigment In-Reply-To: <466EC9D4.5080604@georgetown.edu> Message-ID: <170297.97048.qm@web61225.mail.yahoo.com> As you correctly point out, all those treatments (with potassium permanganate followed by oxalic acid) are quite damaging although if you dilute the reagents several times fold from the original recipe and let them act for longer periods of time, after making sure that your sections are well attached to the slides, maybe you will get the pigment removal with less damage. Ren? J. Vernon Dailey wrote: Hi Histonet, I am trying IHC on some human brain tissue. The region of interest is the substantia nigra. The pigment in the SN cells is interfering with my IHC. I have a few protocols for removing this pigment but they are very damaging to the sections. Does anyone have a protocol that works well for this purpose? Any help would be appreciated. Best, Vernon _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- You snooze, you lose. Get messages ASAP with AutoCheck in the all-new Yahoo! Mail Beta. From mhanna <@t> histosearch.com Tue Jun 12 12:24:29 2007 From: mhanna <@t> histosearch.com (Marvin Hanna) Date: Tue Jun 12 12:24:35 2007 Subject: [Histonet] Histosearch Upgrades Message-ID: <595E6ECD-2D6B-47E8-A9CB-B01624D2C2B5@histosearch.com> Hi Histonetters, Shirley Powell and I have upgraded the Histosearch Search Engine to search over 100 histology related websites, quadrupling it's size, with results using Google relevancy rankings. There are a number of new histology resources on the internet and you can search all of them at once at Histosearch. If anyone knows of any educational histology sites we have missed, please let us know so we can include them in the search engine. We have also set up a histology jobs site and a histology auction site. Job postings are free on the job site thru June. The auction site is open to vendors, histologists who think they've developed a better product, histology labs that want to clean out their closets, histologists who provide histology services and clinical labs who would like to sell their good (but expired) antibodies to researchers. State Societies who would like to use the e-commerce capabilities on the site for registration, t-shirts or other fund raising activities can at no cost. Current job openings include: VP Research and Development - Ikonisys Inc., New Haven, Connecticut Supervisor, ARC Histology - St. Jude Children's Research Hospital, Memphis, Tennessee HISTOTECHNOLOGISTS - ProPath Laboratory, Dallas, Texas Histologist - Skaggs Community Health Center, Branson, Missouri HISTOLOGY TECHNICIAN - Alegent Health, OMAHA, Nebraska Laboratory Workflow Specialist - Aperio Technologies, Vista, California IHC Technician - Central Texas Pathology Laboratory, Waco, Texas Technical Support - Histology - Newcomer Supply, Madison, Wisconsin Best Regards, Marvin Hanna mhanna@histosearch.com www.histosearch.com From gcallis <@t> montana.edu Tue Jun 12 12:59:03 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Jun 12 12:58:39 2007 Subject: [Histonet] Brain Pigment In-Reply-To: <000001c7ad10$4d0fc6b0$6d00a8c0@PremierLab.local> References: <466EC9D4.5080604@georgetown.edu> <000001c7ad10$4d0fc6b0$6d00a8c0@PremierLab.local> Message-ID: <6.0.0.22.1.20070612115721.01b30008@gemini.msu.montana.edu> AEC+ from DAKO or Lab Visions AEC are excellent for peroxidase methods and these two are a bit more sensitive than some other than Chris van der Loos's in house AEC recipe if you prefer to do it yourself. At 10:39 AM 6/12/2007, you wrote: >I'm assuming that your pigment is brown, if that's the case then I would >switch to an alkaline phosphatase detection system with a red chromagen such >as fast red. If that's not then there are other colored chromagens that >could be used. > >Liz > >Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC >Manager >Premier Laboratory, LLC >P.O. Box 18592 >Boulder, CO 80308 >phone (303) 735-5001 >fax (303) 735-3540 >liz@premierlab.com >www.premierlab.com > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From Charlotte.Kopczynski <@t> baycare.org Tue Jun 12 16:04:50 2007 From: Charlotte.Kopczynski <@t> baycare.org (Kopczynski, Charlotte) Date: Tue Jun 12 16:04:56 2007 Subject: [Histonet] Ventana Symphony Message-ID: I was also wondering if there are sites out there using the Ventana Symphony and would care to share feedback. Thanks, Charlotte Kopczynski,HTL(ASCP) Regional Pathology Manager BayCare Laboratories Phone: 727-461-8246 Fax: 727-462-7596 No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. Confidential: This electronic message and all contents contain information from BayCare Health System which may be privileged, confidential or otherwise protected from disclosure. The information is intended to be for the addressee only. If you are not the addressee, any disclosure, copy, distribution or use of the contents of this message is prohibited. If you have received this electronic message in error, please notify the sender and destroy the original message and all copies. From burch007 <@t> mc.duke.edu Tue Jun 12 16:08:38 2007 From: burch007 <@t> mc.duke.edu (James L Burchette) Date: Tue Jun 12 16:08:44 2007 Subject: [Histonet] P53 survey results In-Reply-To: <732905.69344.qm@web61223.mail.yahoo.com> Message-ID: Five responses to the p53 survey. Here are the results: 1. I use Dako's monoclonal p53 (clone DO-7) with Dako's pH 6.0 citrate target retrieval for my HIER. 2. I use Dako's p53 clone DO-7 as well with Dako's Target Retrieval Solutin in a steamer. 3. We use Biocare's p53 at 1:50 with Biocare's Bull's Eye, Diva or BORG for 2" in their Decloaking chamber (pressure cooker). Or we use Ventana's standard CC1 HIER on our VMS BenchMark and XT. 4. Clone from DO-7 from DAKO, diluted 1:80 with pH6 HIER 5. Dako clone DO-7 ph 6 (biocare decloaker ie: pressure cooker)1:100 30/15 using envision plus detection system from Dako and stained on Dako autostainer. Breast cancer as control. Dako clone DO-7 ph 6 (biocare decloaker ie: pressure cooker)1:100 30/15 using envision plus detection system from Dako and stained on Dako autostainer. Breast cancer as control. JB From Mary_Joy <@t> smhwecare.com Tue Jun 12 16:49:29 2007 From: Mary_Joy <@t> smhwecare.com (Mary_Joy@smhwecare.com) Date: Tue Jun 12 16:48:16 2007 Subject: [Histonet] cost analysis H&E stain Message-ID: Has anyone ever done a per slide cost analysi so I'd be interested in your cost per slide if y sharing. Thanks From bjdewe <@t> aol.com Tue Jun 12 17:12:50 2007 From: bjdewe <@t> aol.com (bjdewe@aol.com) Date: Tue Jun 12 17:13:00 2007 Subject: [Histonet] STAT- Animal IHC Workshop /San Francisco In-Reply-To: <549268.21838.qm@web82007.mail.mud.yahoo.com> References: <549268.21838.qm@web82007.mail.mud.yahoo.com> Message-ID: <8C97B55F37367F6-1770-1D18@WEBMAIL-DC12.sysops.aol.com> I N N O V E X B I O S C I E N C E S WORKSHOP ANNOUNCEMENT Animal Tissue STAT-IHC staining workshop >From primary to microscope under 2 hours ? PRESENTED by Innovex Biosciences ? San Francisco, Saturday August 18, 2007, 1:00-4:30 pm LEVEL: Open to all levels ?? This is a comprehensive hands-on wet workshop designated to address the major issues facing the veterinary Histologists that perform animal tissue IHC staining.? The topics covered will include eradication of background staining in animal tissues, the techniques for lowering the primary antibody incubation time and lowering the entire staining run for fast turn-around time. Other topics covered will include the selection of appropriate secondary antibodies and co-reagents for IHC staining of animal tissues. This workshop will further address the STAT staining technology available for background ?free quick IHC staining of animal tissues in less than 2 hours. Other covered topics will include novel amplification technologies for maximizing optimal staining results and minimizing troubleshooting. The application of STAT-IHC reagents and automated stainers will also be explored. This workshop is CEU approved CEU: This workshop is CEU approved for 3 contact hours? FEE: This workshop is offered free of charge BENEFICIAL TO: Veterinarian pathologists and histologists, Veterinary IHC Practitioners, Veterinary researchers, Research histotechnologists and research immunohistochemists and to all Cost and time conscious Labs WORKSHOP INSTRUCTORS: Zahra Naser, Ph.D.; M.S, with over 20 years of experience in Immunology, IHC, flow cytometry and Biochemistry and chemistry. Loralei Dewe: Co-Director of Confocal Microscope Core at Shriners Hospital, 10+ years experience with Histology, Immunochemistry and Light and Fluorescent Imaging. ? SPACE: Space is limited to 22 people for maximum personal attention. Reserve your space as soon as possible. ? LOCATION: San Francisco, University of California, San Francisco (UCSF), Laurel heights campus, Parking: Parking available for a fee. ? DATE:?? Saturday August 18, 2007, 1:00-4:30 pm?????????????????? TO REGISTER On line, Visit innvx.com, click on Register for workshop on home page. TO REGISTER via Phone:? Call us at 1-800-622-7808, Main 510-234-6600. TO REGISTER via fax: Fill out the enclosed registration form and fax it to 510-234-4591. HOTEL INFORMATION: If you require accommodation, please contact Innovex immediately.? Also upon registration, hotel and transportation will be emailed or Faxed to you. Contact Innovex at 1-800-622-7808 OR 510-234-6600 Web: innx.com Disclaimer: Innovex reserves all rights for admission of the participants. ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From histotech <@t> charter.net Tue Jun 12 20:59:26 2007 From: histotech <@t> charter.net (histotech@charter.net) Date: Tue Jun 12 20:59:29 2007 Subject: [Histonet] Xylene Substitute Message-ID: <2014889587.1181699966558.JavaMail.root@fepweb03> Have used Formula 83 for 3 years now with absolutely no problems - wish I had changed years ago. DDietz Morristown-Hamblen Hospital ---- "Molinari wrote: > Hi Rebecca, > I changed from Xylene to Formula 83 for my stainer/coverslipper and I > have had no problems. Definitely less fumes! > > Betsy Molinari HT (ASCP) > Texas Heart Institute > Cardiovascular Pathology > 6770 Bertner Ave. > Houston,TX 77030 > 832-355-6524 > 832-355-6812 (fax) > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rebecca > Jones > Sent: Monday, June 11, 2007 10:41 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Xylene Substitute > > I know this topic has been on here before, but of course I paid little > attention as it wasn't on top of my busy list! And I rarely converse > over > the histonet, but definately watch as the conversations move along. > > I work in a small research lab, but recently got a call from CBG Biotech > to > try their xylene substitute, Formula 83 for free. > > I have only ever used xylene, but am fairly new to histologic procecures > so > I am considering an alternative that is less toxic. > > Has anyone tried Formula 83 or have any other substitutes they can > suggest. > > > I will try my hardest to post the results of my testing so that everyone > can > know. I think once I get the sample in I can test things fairly > quickly. I > would like to sample more than one though, just to have a comparisson. > > Thanks, > > Rebecca Jones > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mbmphoto <@t> gmail.com Tue Jun 12 22:25:50 2007 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Tue Jun 12 22:24:15 2007 Subject: [Histonet] coating 50mmx75mm brain glass slides Message-ID: <62ABEB06-B91D-4408-B83D-DAF64D4C14EE@gmail.com> Hello, I'm looking for a vendor that sells superfrost plus or polysine or any other coated 50mmx75mm brain glass slides WITHOUT the frosted edge. In other words, I need plain glass slides that are coated and measure 50mmx75mm. Other than doing the coating myself - which I rather not do because we do a lot of sections and really don't the time to coat. Please if anyone can help let me know. Any information you can provide will be greatly appreciated. Maria Bartola Mejia UCSF Department of Neurosurgery SF CA 94103 From nagorsn <@t> mail.biu.ac.il Wed Jun 13 23:28:01 2007 From: nagorsn <@t> mail.biu.ac.il (Natalie Nagorsky) Date: Wed Jun 13 01:30:29 2007 Subject: [Histonet] TH staining Message-ID: <000601c7ae3c$658e16b0$5e284684@natali> Hi Histonet, I have several protocols for Tirosine Hydroxylase and I was not successful to get good results. May some one share the working protocol and sours for TH. Any help will be appreciate. Natalie Brain Research Center Bar-Ilan University Israel From louise.renton <@t> gmail.com Wed Jun 13 06:24:38 2007 From: louise.renton <@t> gmail.com (louise renton) Date: Wed Jun 13 06:24:47 2007 Subject: [Histonet] IHC background Message-ID: Hi all, This may have been asked before, but i'm struggling to find reference to it. I have a problem with high background when doing IHC on retrospective tisue blocks. The blocks (soft tissue, bone and hydroxyapatite implants)were fixed in alcohol and decalcified in a home brew of formic acid and HCl. The tissue is primate, the antibodies anti human rabbit polyclonals. I have optimised the antibodies to tissue from the same species (formalin fixed and if necessary decalcified electrolytically in Sakura's TDE soln normal overnight processing to wax). I have also run the antibodies on the same TYPE of sample as the retro project (but one where the fixation and decalcification was controlled by me) and got good clean crisp staining in most of the right places. Some staining in serum in blood vessels but could be excluded on morphology. Brief method: dewax Quench in metghanol/H2o2 Normal horse serum block primary overnight at 4 deg C Pan secondary antibody (Vector) biotinylated ABC kit detection/amplification DAB My problem is this: In the retro blocks there is HUGe baxckground, of a muddy dark brown colour (instread of the well advertised golden brown pigment) Everything stains, muscle, bone, bone cells, collagen. Is there any way to retrieve the situation? BSA in the wash buffer? Detergents? I have tried using the Novolink kit acording to their protocol and lost all staining I have access to a Vector Elite kit but have not tried it yet Any suggestions?. I am ready to tear my hair, kick the desk and cause general mayhem by stealing everybody's left shoe. with very best regards -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From Janet.Keeping <@t> cna.nl.ca Wed Jun 13 07:06:17 2007 From: Janet.Keeping <@t> cna.nl.ca (Keeping, Janet) Date: Wed Jun 13 07:06:29 2007 Subject: [Histonet] Poor Eosin staining after prolonged fixation Message-ID: Has anyone experienced poor eosin counterstaining using tissues which have had very prolonged fixation in neutral buffered formalin? The tissues I have for students have been fixed for a very long time. (not generally a problem in the clinical site). Although the pH of the stain is between 4.6-5.0, the slides are well washed following bluing, time in 70% alcohol for differentiation is minimal and eosin staining times have been increased the counterstaining is still poor. I am guessing that the tissue groups that bind with eosin are not accessible due to continued cross linking. Any ideas or suggestions? Janet Keeping A.R.T., B. Voc. Ed Instructor Medical Laboratory Sciences 1 Prince Philip drive P.O. Box 1693, St. John's NL, Canada A1C 5P7 709 758 7657 tel 709 758 7635 fax E-mail; janet.keeping@cna.nl.ca From valeria.berno <@t> embl.it Wed Jun 13 07:34:08 2007 From: valeria.berno <@t> embl.it (Valeria Berno) Date: Wed Jun 13 07:34:15 2007 Subject: [Histonet] neurons Message-ID: Hi all, I am new in the list and I already found it very useful so I hope to solve this issue as well even if maybe is not "histology"related..... I am working on primary neuronal cell culture trying to stain a membrane receptor-GFP (with an anti-GFP antibody) and the MAPK activation. Problems: 1- in the same coverslip if the antiGFP works...the anti MAPK doesn't!(and viceversa). They are Mouse and Rabbit respectively and I am incubating them together (milk 5% in TBS-T). 2- Where I have the membrane receptor working however I would like to see the internalization: I am fixing 15min in PFA4% on ice and permeabilizing 30min 0.2%triton But I don't get a good idea of the localization. Is that ok as protocol or it is too strong? Thanks in advance for all your suggestions or references Valeria Berno Valeria, PhD EMBL Monterotondo Outstation via Ramarini 32 00015 Monterotondo Scalo (RM) Italy Tel: +39 06 90091 287 Fax: +39 06 90091 272 HYPERLINK "mailto:valeria.berno@embl.it"valeria.berno@embl.it No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.5.472 / Virus Database: 269.8.15/847 - Release Date: 12/06/2007 21.42 From rjbuesa <@t> yahoo.com Wed Jun 13 08:54:20 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jun 13 08:54:23 2007 Subject: [Histonet] cost analysis H&E stain In-Reply-To: Message-ID: <426556.15101.qm@web61225.mail.yahoo.com> Mary Under separate cover I am sending a paper I wrote on costs tha include H&E, totally manual vs. with automated steps, from cassette to finished slide and other variables. Ren? J. Mary_Joy@smhwecare.com wrote: Has anyone ever done a per slide cost analysi= s for H&E staining? If so I'd be interested in your cost per slide if y= ou wouldn't mind sharing. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. From PMonfils <@t> Lifespan.org Wed Jun 13 09:52:09 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Jun 13 09:52:19 2007 Subject: [Histonet] coating 50mmx75mm brain glass slides In-Reply-To: <62ABEB06-B91D-4408-B83D-DAF64D4C14EE@gmail.com> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273C9B@LSRIEXCH1.lsmaster.lifespan.org> I believe they can be purchased from brain research laboratories. http://www.brainresearchlab.com/productinform.html From TJJ <@t> Stowers-Institute.org Wed Jun 13 12:37:57 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Wed Jun 13 12:38:29 2007 Subject: [Histonet] Re: IHC background Message-ID: Louise, You are using a protocol (including antibody concentrations) that is optimized for use on formalin fixed tissues which have been TDE decalcified, and it's not working well on alcohol fixed tissues decalcified in formic acid/HCl. That's the problem. Questions to ask: 1 - are you seeing any specific positive signal in the midst of the background on the alcohol fixed samples? 2 - when you tried the Novolink kit, did you lose all staining in the alcohol fixed samples, or also in your formalin fixed samples? Some things to consider: The antigen may not be conformationally preserved using alcohol. So, it's possible the antibody does not recognize the alcohol-fixed protein, but does recognize and bind with the formalin fixed one. The decalcification with Formic/HCl may have additionally altered the protein, and perhaps what you're looking for isn't even in there any more! Alcohol fixed tissues generally do not require any retrieval, since alcohol does not cross-link or coagulate proteins. You might try using the same detection method, but dilute out your primary antibody concentration (use 2-3 serial dilutions from what you currently use) to try to lower the background. If you see no specific signal in any of your antibody stainings, you may think about perhaps a different antibody (polyclonal vs monoclonal) that may recognize the protein in alcohol fixed samples. Good luck! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From: "louise renton" Subject: [Histonet] IHC background To: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hi all, This may have been asked before, but i'm struggling to find reference to it. I have a problem with high background when doing IHC on retrospective tisue blocks. The blocks (soft tissue, bone and hydroxyapatite implants)were fixed in alcohol and decalcified in a home brew of formic acid and HCl. The tissue is primate, the antibodies anti human rabbit polyclonals. I have optimised the antibodies to tissue from the same species (formalin fixed and if necessary decalcified electrolytically in Sakura's TDE soln normal overnight processing to wax). I have also run the antibodies on the same TYPE of sample as the retro project (but one where the fixation and decalcification was controlled by me) and got good clean crisp staining in most of the right places. Some staining in serum in blood vessels but could be excluded on morphology. Brief method: dewax Quench in metghanol/H2o2 Normal horse serum block primary overnight at 4 deg C Pan secondary antibody (Vector) biotinylated ABC kit detection/amplification DAB My problem is this: In the retro blocks there is HUGe baxckground, of a muddy dark brown colour (instread of the well advertised golden brown pigment) Everything stains, muscle, bone, bone cells, collagen. Is there any way to retrieve the situation? BSA in the wash buffer? Detergents? I have tried using the Novolink kit acording to their protocol and lost all staining I have access to a Vector Elite kit but have not tried it yet Any suggestions?. I am ready to tear my hair, kick the desk and cause general mayhem by stealing everybody's left shoe. with very best regards -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From LSPrestridge <@t> seton.org Wed Jun 13 12:54:13 2007 From: LSPrestridge <@t> seton.org (Prestridge, Linda S.) Date: Wed Jun 13 12:54:19 2007 Subject: [Histonet] Magnet for old VIP's Message-ID: <3D79F47DC92B204F9E5D35C885DFC5CB014338C2@AUSEX2VS1.seton.org> Does anyone have a magnet to use on the VIP they no longer use. If you can let go of it, please mail to Seton Medical Center, 1201 W.38th St., Austin, TX 78705. Send to Linda Prestridge, Histology Dept. Thanks, LP From gcallis <@t> montana.edu Wed Jun 13 13:11:55 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed Jun 13 13:11:26 2007 Subject: [Histonet] Magnet for old VIP's In-Reply-To: <3D79F47DC92B204F9E5D35C885DFC5CB014338C2@AUSEX2VS1.seton.o rg> References: <3D79F47DC92B204F9E5D35C885DFC5CB014338C2@AUSEX2VS1.seton.org> Message-ID: <6.0.0.22.1.20070613120753.01b22100@gemini.msu.montana.edu> Linda, You do not need the little pricey gray magnet - just use a magnetic teflon coated stirring bar of the same size. When we bought our VIP K series in 1984, Miles aka Sakura told us to substitute with the stirring bar. Curiousity made me try it and it worked nicely. There are some stirring bars that do not have ridges for closer contact, but the others work too. Have fun At 11:54 AM 6/13/2007, you wrote: >Does anyone have a magnet to use on the VIP they no longer use. If you >can let go of it, please mail to Seton Medical Center, 1201 W.38th St., >Austin, TX 78705. Send to Linda Prestridge, Histology Dept. >Thanks, >LP Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From poulea34 <@t> hotmail.com Wed Jun 13 13:53:34 2007 From: poulea34 <@t> hotmail.com (Liz Poulette) Date: Wed Jun 13 13:53:39 2007 Subject: [Histonet] Are You Paid for Grossing and Autopsy Assistance? Message-ID: Hello All! Just like the subject says: any techs who assist with grossing and autopsy: are you getting paid for the extra work/responsibility? If you are -could you tell me what you are getting paid? thanks! Liz _________________________________________________________________ With Windows Live Hotmail, you can personalize your inbox with your favorite color. www.windowslive-hotmail.com/learnmore/personalize.html?locale=en-us&ocid=TXT_TAGLM_HMWL_reten_addcolor_0607 From mpence <@t> grhs.net Wed Jun 13 14:07:24 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Jun 13 14:07:33 2007 Subject: [Histonet] Are You Paid for Grossing and Autopsy Assistance? In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C62E@IS-E2K3.grhs.net> You need to clarify to what degree of assisting. Some many do all the grossing, a prosector, and some may just snap lids. Likewise, to what degree of autopsy assisting. Some many assist with the pathologist or resident and others may be a diener. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Poulette Sent: Wednesday, June 13, 2007 1:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Are You Paid for Grossing and Autopsy Assistance? Hello All! Just like the subject says: any techs who assist with grossing and autopsy: are you getting paid for the extra work/responsibility? If you are -could you tell me what you are getting paid? thanks! Liz _________________________________________________________________ With Windows Live Hotmail, you can personalize your inbox with your favorite color. www.windowslive-hotmail.com/learnmore/personalize.html?locale=en-us&ocid =TXT_TAGLM_HMWL_reten_addcolor_0607_____________________________________ __________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cindipqr <@t> wowway.com Wed Jun 13 14:19:09 2007 From: cindipqr <@t> wowway.com (cindipqr@wowway.com) Date: Wed Jun 13 14:19:16 2007 Subject: [Histonet] -80 freezer Message-ID: <20070613191506.M66115@wowway.com> Hi All, I'm in the market for a -80 freezer. Does anyone have any opinions- pro or con? Thanks, Cindi HFH Detroit, Mi From rjbuesa <@t> yahoo.com Wed Jun 13 14:36:53 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jun 13 14:36:59 2007 Subject: [Histonet] Magnet for old VIP's In-Reply-To: <3D79F47DC92B204F9E5D35C885DFC5CB014338C2@AUSEX2VS1.seton.org> Message-ID: <417782.49462.qm@web61215.mail.yahoo.com> Almost any magnet "horse-shoe" type can be used. Ren? J. "Prestridge, Linda S." wrote: Does anyone have a magnet to use on the VIP they no longer use. If you can let go of it, please mail to Seton Medical Center, 1201 W.38th St., Austin, TX 78705. Send to Linda Prestridge, Histology Dept. Thanks, LP _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Luggage? GPS? Comic books? Check out fitting gifts for grads at Yahoo! Search. From sbledsoe <@t> iupui.edu Wed Jun 13 15:31:24 2007 From: sbledsoe <@t> iupui.edu (Sharon Bledsoe) Date: Wed Jun 13 15:31:33 2007 Subject: [Histonet] -80 freezer In-Reply-To: <20070613191506.M66115@wowway.com> References: <20070613191506.M66115@wowway.com> Message-ID: Cindi, Yes, I do indeed, have an opinion. We recently bought a Revco 24.4 cu ft Ultra II, it was so loud that we had to buy a sound abatement kit and had to have someone from the company come to install it. It's nice, works great, but it is LOUD. Sharon At 2:19 PM -0500 6/13/07, cindipqr@wowway.com wrote: >Hi All, >I'm in the market for a -80 freezer. Does anyone have any opinions- pro or >con? >Thanks, >Cindi >HFH >Detroit, Mi > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dusko.trajkovic <@t> pfizer.com Wed Jun 13 16:26:59 2007 From: dusko.trajkovic <@t> pfizer.com (Trajkovic, Dusko) Date: Wed Jun 13 16:27:08 2007 Subject: [Histonet] Some IHC/RNA advice Message-ID: <3AD0BD3142459B4E9B12CBEAFF2B89B204A366F6@lajamrexm01.amer.pfizer.com> Hallo everyone, A colleague of mine is looking for information which I could not help him with. If anyone out there could give him any answers, both of us would be very grateful. You can correspond directly with him or you can send the info trough my email and I will forward it to him. Thank you Dusko Trajkovic -----Original Message----- From: Yun Yung [mailto:yyung@scripps.edu] Sent: Wednesday, June 13, 2007 2:03 PM To: Trajkovic, Dusko Subject: some IHC/RNA advice Hi Dusko, Hope things are going well...haven't gotten a chance to talk to you for a while now. I hope you and Natasha will be able to make it to my b-day party. By the way, do you know of anyone who has successfully preserved RNA in tissue with RNA later, then taken the tissue to stain using IHC, then analyzed the RNA content using microarray? I'm curious what you think about IHC with tissue that has been preserved with RNA later. Any help would be appreciated. Thanks! Yun Yun C. Yung Chun Lab, Molecular Biology The Scripps Research Institute 10550 North Torrey Pines Rd. ICND-118 La Jolla, CA 92037 yyung@scripps.edu (858) 784-1000 ext 4-3123 ---------------------------------------------------------------------- LEGAL NOTICE Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this E-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately. From poulea34 <@t> hotmail.com Wed Jun 13 16:34:12 2007 From: poulea34 <@t> hotmail.com (Liz Poulette) Date: Wed Jun 13 16:34:18 2007 Subject: [Histonet] more specific information included-Are You Paid for Grossing and Autopsy Assistance? Message-ID: ORIGIONAL QUESTION Hello All! Just like the subject says: any techs who assist with grossing and autopsy: are you getting paid for the extra work/responsibility? If you are -could you tell me what you are getting paid? thanks! Liz MORE SPECIFIC At the hospital I work at I gross everything but large complex cancer cases. I fill in for the PA when she is on vacation and/or when there are alot of specimens. I assist the PA with autopsy cases. I help remove organs, remove brains, clean up and keep the morgue stocked and in order. I work at a smaller hospital so I don't so this on a regular basis. I am a graduate of a histology program here in NY/ascp certified and also have a BA in Anthropology. I don't get paid for the extra work i do..it falls under "other duties assigned" . I don't mind because I don't do any of the above on a regular basis-its good to have skills that can transfer to another job if need be though. I am just curious what techs who do get paid-how much work they do and what the going rate is. Thanks again! Liz _________________________________________________________________ Live Earth is coming.? Learn more about the hottest summer event - only on MSN. http://liveearth.msn.com?source=msntaglineliveearthwlm From Jeannette.Mitchell <@t> vtmednet.org Wed Jun 13 16:36:23 2007 From: Jeannette.Mitchell <@t> vtmednet.org (Mitchell, Jeannette M.) Date: Wed Jun 13 16:36:28 2007 Subject: [Histonet] Region I Symposium 2008 Message-ID: <8C7B2CE85A8CBA49B3FE05DE478412ED029DC6C2@FAHC14.fahc.fletcherallen.org> Please visit the web site below and click on Vermnot/New Hampshire to view infomation for the Meeting 2008. http://www.geocities.com/nshregion1/index.html Thank you Jeannette Confidentiality Notice: This message, and any attachments, may contain information that is confidential, privileged, and/or protected from disclosure under state and federal laws that deal with the privacy and security of medical information. If you received this message in error or through inappropriate means, please reply to this message to notify the Sender that the message was received by you in error, and then permanently delete this message from all storage media, without forwarding or retaining a copy. From Erin.Martin <@t> ucsf.edu Wed Jun 13 18:01:21 2007 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Wed Jun 13 18:02:58 2007 Subject: [Histonet] JCAHO & CAP Message-ID: Hello, My manager asked me to explain why we need CAP if we are a JCAHO hospital. We just started a dermpath lab, separate from the rest of the pathology department and this is my first time in a non-CAP lab. In other facilities I have worked, I just took for granted that CAP surveys were a necessity. Now I'm not so sure - can anyone tell me if CAP surveys/inspections are necessary? I was under the impression that JCAHO and CAP sort of worked in concert to confirm a lab's compliance with all CLIA regs, but I can't find anything to support that assumption. I know that under CMS some sort of lab accreditation is required, but on the CMS site they list both CAP and JCAHO as accrediting agencies (among others). So why both? Is it necessary to have CAP if the hospital is JCAHO? Thank you, Erin Martin From sheila_adey <@t> hotmail.com Wed Jun 13 18:20:27 2007 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Wed Jun 13 18:20:33 2007 Subject: [Histonet] Are You Paid for Grossing and Autopsy Assistance? In-Reply-To: <661949901A768E4F9CC16D8AF8F2838CA1C62E@IS-E2K3.grhs.net> Message-ID: The techs in our dept were just discussing this issue today. We gross all the "small stuff" G.I , fallopian tubes, tonsils, currettings, TURPs, etc. We were wondering since some HTs don't have to do Cyto or Grossing; if there was a difference in pay scales. We do all of this and the top of our scale is 23.91. We would like to know if this falls within other institutions pay range and job description. Thanks Sheila Adey HT MLT Port Huron Hospital Michigan >From: "Mike Pence" >To: "Liz Poulette" , > >Subject: RE: [Histonet] Are You Paid for Grossing and Autopsy Assistance? >Date: Wed, 13 Jun 2007 14:07:24 -0500 > >You need to clarify to what degree of assisting. Some many do all the >grossing, a prosector, and some may just snap lids. Likewise, to what >degree of autopsy assisting. Some many assist with the pathologist or >resident and others may be a diener. > >Mike > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz >Poulette >Sent: Wednesday, June 13, 2007 1:54 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Are You Paid for Grossing and Autopsy Assistance? > > > >Hello All! > >Just like the subject says: any techs who assist with grossing and >autopsy: are you getting paid for the extra work/responsibility? If you >are -could you tell me what you are getting paid? > >thanks! >Liz > > >_________________________________________________________________ >With Windows Live Hotmail, you can personalize your inbox with your >favorite color. >www.windowslive-hotmail.com/learnmore/personalize.html?locale=en-us&ocid >=TXT_TAGLM_HMWL_reten_addcolor_0607_____________________________________ >__________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Windows Live Hotmail with drag and drop, you can easily move and organize your mail in one simple step. Get it today! www.newhotmail.ca?icid=WLHMENCA153 From jnocito <@t> satx.rr.com Wed Jun 13 18:27:07 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Jun 13 18:27:11 2007 Subject: [Histonet] Magnet for old VIP's References: <417782.49462.qm@web61215.mail.yahoo.com> Message-ID: <00f101c7ae12$5c02cc00$d49eae18@yourxhtr8hvc4p> not only those, but I purchased some round magnets and those work too. I have to slide the magnet back and forth a little, but how can you go wrong for $1.99? Joe, I'm unemployed for now, the Toe. ----- Original Message ----- From: "Rene J Buesa" To: "Prestridge, Linda S." ; Sent: Wednesday, June 13, 2007 2:36 PM Subject: Re: [Histonet] Magnet for old VIP's > Almost any magnet "horse-shoe" type can be used. > Ren? J. > > "Prestridge, Linda S." wrote: > Does anyone have a magnet to use on the VIP they no longer use. If you > can let go of it, please mail to Seton Medical Center, 1201 W.38th St., > Austin, TX 78705. Send to Linda Prestridge, Histology Dept. > Thanks, > LP > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Luggage? GPS? Comic books? > Check out fitting gifts for grads at Yahoo! Search. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Wed Jun 13 18:30:09 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Jun 13 18:30:14 2007 Subject: [Histonet] JCAHO & CAP References: Message-ID: <010501c7ae12$c8b4b980$d49eae18@yourxhtr8hvc4p> Erin, no, you do not need both. One of the inspectors I had a couple of years said during the inspection that he was transferring his lab from CAP to JCAHO. He was getting tired of the political BS with CAP. Hmmm. I like his views. Joe ----- Original Message ----- From: "Martin, Erin" To: Sent: Wednesday, June 13, 2007 6:01 PM Subject: [Histonet] JCAHO & CAP Hello, My manager asked me to explain why we need CAP if we are a JCAHO hospital. We just started a dermpath lab, separate from the rest of the pathology department and this is my first time in a non-CAP lab. In other facilities I have worked, I just took for granted that CAP surveys were a necessity. Now I'm not so sure - can anyone tell me if CAP surveys/inspections are necessary? I was under the impression that JCAHO and CAP sort of worked in concert to confirm a lab's compliance with all CLIA regs, but I can't find anything to support that assumption. I know that under CMS some sort of lab accreditation is required, but on the CMS site they list both CAP and JCAHO as accrediting agencies (among others). So why both? Is it necessary to have CAP if the hospital is JCAHO? Thank you, Erin Martin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Wed Jun 13 18:44:52 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Jun 13 18:44:55 2007 Subject: [Histonet] Black Precipitate Message-ID: <012301c7ae14$d6d8cd60$d49eae18@yourxhtr8hvc4p> Hheeeeeeeeeeeellllllllllllllllllllpppppppppppppppp. Please I've never seen this in all my years of IHC, even though I've been around the block once or twice. Yesterday, we ran a CD panel on a bone marrow biopsy on a Ventana Benchmark XT. The same tonsil control was used for CD-3, CD-5, CD-20 and CD-34. The patient tissue was on the same slide as the controls. When we were QCing the slides, there was a black, crystalline precipitate on the CD-34 control, but not on the patient tissue. It was just on the tissue, but not surrounding the tissue. All other slides from the case were fine. Several other cases were run at the same time, but didn't have any precipitate. The biopsy was B-plus fixed and decaled in IED solution from Biocare Medical, which, in my opinion, (and everyone knows about opinions) has been the best decal solution for immunos. Even my hempath loves it and we don't agree on anything. These solutions have been used in conjunction for months and never had this problem. Any ideas? I'm about to crack the seal on a new bottle of Chivas Scotch and ponder the situation. Before I got too into the bottle, I wanted to ask the experts. Thanks in advance and bottoms up! Joe From Lchausse <@t> nmh.org Wed Jun 13 18:48:41 2007 From: Lchausse <@t> nmh.org (Chaussey, Leslie) Date: Wed Jun 13 18:49:26 2007 Subject: [Histonet] -80 freezer Message-ID: I've had good performance with Sanyo models. You can see them on the Sanyo web site. ----- Original Message ----- From: histonet-bounces@lists.utsouthwestern.edu To: cindipqr@wowway.com ; Histonet Sent: Wed Jun 13 15:31:24 2007 Subject: Re: [Histonet] -80 freezer Cindi, Yes, I do indeed, have an opinion. We recently bought a Revco 24.4 cu ft Ultra II, it was so loud that we had to buy a sound abatement kit and had to have someone from the company come to install it. It's nice, works great, but it is LOUD. Sharon At 2:19 PM -0500 6/13/07, cindipqr@wowway.com wrote: >Hi All, >I'm in the market for a -80 freezer. Does anyone have any opinions- pro or >con? >Thanks, >Cindi >HFH >Detroit, Mi > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- This message and any included attachments are intended only for the addressee. The information contained in this message is confidential and may constitute proprietary or non-public information under international, federal, or state laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail. From talulahgosh <@t> gmail.com Wed Jun 13 21:48:25 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Wed Jun 13 21:48:30 2007 Subject: [Histonet] -80 freezer In-Reply-To: References: Message-ID: We have a Forma--no problems with it since we bought it nine years ago. I would suggest an upright because it saves room. Also, our upright has two large chambers with separate doors, which are both separated into two shelves. This keeps half of the freezer cold while you open the door for the other half, something not available in the chest-type freezer. Emily -- penguins, spoons, and you--what's life like among the flightless? http://www.getafirstlife.com/ From Andrew.Prior <@t> Smith-Nephew.com Thu Jun 14 08:13:36 2007 From: Andrew.Prior <@t> Smith-Nephew.com (Prior, Andrew) Date: Thu Jun 14 08:13:45 2007 Subject: [Histonet] Technovit 7200VLC Message-ID: <7028DD15E14FDC4DB1F3C5AF8735AF37020BBEDC@EHS021.wound.san> Dear Histonetters, I am looking for other people that work with Technovit 7200VLC resin on large (25x25x4mm) samples. At the moment the infiltration takes 3 days for each of the graded stages (30-100%) and the final 100% resin step takes up to 18 days. This is what is recommended by the manufacturers of the Exakt system we use, but seems rather a long time! We have tried reducing the times but have ended up with poor section quality. Unfortunately we cannot reduce the sample size as serial sections have to be cut. Does anyone out there have any experience with this resin, and have a method to reduce the infiltration times? Any suggestions greatly appreciated. Thanks in advance, Andrew Andrew Prior Histologist Smith &Nephew Research Centre York Science Park Heslington York YO10 5DF UK Andrew.Prior@smith-nephew.com Confidentiality. This electronic transmission is strictly confidential to Smith & Nephew and intended solely for the addressee. It may contain information which is covered by legal, professional or other privilege. If you are not the intended addressee, or someone authorised by the intended addressee to receive transmissions on behalf of the addressee, you must not retain, disclose in any form, copy or take any action in reliance on this transmission. If you have received this transmission in error, please notify the sender as soon as possible and destroy this message. Smith & Nephew UK Limited Registered in England and Wales No.4421171 with registered office at 15 Adam Street, London WC2N 6LA From mcauliff <@t> umdnj.edu Thu Jun 14 08:46:45 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Jun 14 08:47:41 2007 Subject: [Histonet] Poor Eosin staining after prolonged fixation In-Reply-To: References: Message-ID: <467146C5.20206@umdnj.edu> Hi Janet: I recall hearing of this problem and I looked in my old copy of Humason's text to find the solution. Naturally, the solution was not there! However, I suggest a short oxidation with 0.25% potassium permanganate followed by bleaching with 0.5% oxalic acid for a minute or two until the sections are colorless. Wash thoroughly. This should improve the staining, but be careful it may improve it too much! Geoff Keeping, Janet wrote: > Has anyone experienced poor eosin counterstaining using tissues which > have had very prolonged fixation in neutral buffered formalin? The > tissues I have for students have been fixed for a very long time. (not > generally a problem in the clinical site). Although the pH of the stain > is between 4.6-5.0, the slides are well washed following bluing, time in > 70% alcohol for differentiation is minimal and eosin staining times > have been increased the counterstaining is still poor. I am guessing > that the tissue groups that bind with eosin are not accessible due to > continued cross linking. Any ideas or suggestions? > > > > Janet Keeping A.R.T., B. Voc. Ed > > Instructor > > Medical Laboratory Sciences > > > > 1 Prince Philip drive > > P.O. Box 1693, St. John's > > NL, Canada A1C 5P7 > > 709 758 7657 tel > > 709 758 7635 fax > > E-mail; janet.keeping@cna.nl.ca > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From Janet.Keeping <@t> cna.nl.ca Thu Jun 14 09:20:37 2007 From: Janet.Keeping <@t> cna.nl.ca (Keeping, Janet) Date: Thu Jun 14 09:20:52 2007 Subject: [Histonet] Poor eosin staining following prolonged fixation - summary Message-ID: Thanks you to all the people who responded. A summary of the suggestions I received follow: * Following bluing-use 1 minute water wash followed by 70% alcohol then place in Eosin * No 70% alcohol after Eosin, 95% is dilute enough * Use fresher tissues * Add phloxine to the Eosin * Treat slides with permanganate and oxalic acid before staining * Check whether Eosin is water or alcohol soluble Janet Keeping A.R.T., B. Voc. Ed Instructor Medical Laboratory Sciences 1 Prince Philip drive P.O. Box 1693, St. John's NL, Canada A1C 5P7 709 758 7657 tel 709 758 7635 fax E-mail; janet.keeping@cna.nl.ca From froyer <@t> bitstream.net Thu Jun 14 09:55:04 2007 From: froyer <@t> bitstream.net (Ford Royer) Date: Thu Jun 14 09:55:15 2007 Subject: [Histonet] Magnet for old VIP's In-Reply-To: <00f101c7ae12$5c02cc00$d49eae18@yourxhtr8hvc4p> References: <417782.49462.qm@web61215.mail.yahoo.com> <00f101c7ae12$5c02cc00$d49eae18@yourxhtr8hvc4p> Message-ID: <002801c7ae93$fe2ebd50$7701a80a@Ford> What Joe is describing is the proper alignment of the magnetic polarity. The magnetic switches on the VIP have a North and South pole just like all magnets. Any magnet with enough strength can be used to activate the magnetic switches but you may have to turn or move the magnet around to match it to poles on the switch. Also Gayle mentioned using a magnetic stir bar which will work if it is strong enough, but it may not ?stick? in place to free you from having to hold it in place while you try to operate the key pad. A small sized horseshoe magnet like Rene mentioned might work better and stay in place by itself. In an emergency one time I found one at a kid?s toy store that was the right size and strength to activate the switch. Refrigerator magnets of the right strength will also work. ~ Ford Ford M. Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL:? 612-839-1046 Phone:? 763-542-8725 Fax:? 763-546-4830 eMail:? clinicallab@minnesotamedical.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, June 13, 2007 6:27 PM To: Rene J Buesa; Prestridge, Linda S.; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Magnet for old VIP's not only those, but I purchased some round magnets and those work too. I have to slide the magnet back and forth a little, but how can you go wrong for $1.99? Joe, I'm unemployed for now, the Toe. ----- Original Message ----- From: "Rene J Buesa" To: "Prestridge, Linda S." ; Sent: Wednesday, June 13, 2007 2:36 PM Subject: Re: [Histonet] Magnet for old VIP's > Almost any magnet "horse-shoe" type can be used. > Ren? J. > > "Prestridge, Linda S." wrote: > Does anyone have a magnet to use on the VIP they no longer use. If you > can let go of it, please mail to Seton Medical Center, 1201 W.38th St., > Austin, TX 78705. Send to Linda Prestridge, Histology Dept. > Thanks, > LP > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Luggage? GPS? Comic books? > Check out fitting gifts for grads at Yahoo! Search. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gentras <@t> vetmed.auburn.edu Thu Jun 14 11:08:10 2007 From: gentras <@t> vetmed.auburn.edu (Atoska Gentry) Date: Thu Jun 14 11:08:18 2007 Subject: [Histonet] cutscum Message-ID: <467167EA.5080308@vetmed.auburn.edu> hello, one of my co-workers needs the name of a neutral detergent referred to as cutscum in an IHC protocol from the late 70's. If anyone has an idea of the actual brand name of this detergent will you please share it with me ASAP? Thanks, Atoska -- Atoska S. Gentry, B.S., HT(ASCP) Research Assistant IV Scott-Ritchey RSCH Center College of Vet. Med Auburn, AL 36849 PH (334) 844-5579 FAX (334) 844-5850 email: gentras@vetmed.auburn.edu From MAUGER <@t> email.chop.edu Thu Jun 14 11:11:33 2007 From: MAUGER <@t> email.chop.edu (Joanne Mauger) Date: Thu Jun 14 11:12:12 2007 Subject: [Histonet] TH staining Message-ID: Hi All, We use TH from Novocastra(now Visionbiosystems) mouse monoclonal at 1:80. We use pH 10 retrieval buffer. It works with ABC Vector and Dako Envision dual detection systems. Jo Mauger From FUNKM <@t> mercyhealth.com Thu Jun 14 11:26:53 2007 From: FUNKM <@t> mercyhealth.com (Marcia Funk) Date: Thu Jun 14 11:27:19 2007 Subject: [Histonet] IHC precipitate Message-ID: <467125FD020000AC00003519@nodcmsngwia1.trinity-health.org> Joe, The scotch didn't help and I'm still trying to figure it out. We have a Ventana Benchmark and XT, the same crystalling preciptate on the same panel. Never has happened again and I really did not come up with a sound reason. I have looked into everything I could think of why it might have happened. Marcia From gcallis <@t> montana.edu Thu Jun 14 11:56:03 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Jun 14 11:55:36 2007 Subject: [Histonet] Biocontainers for carrying vials in and out of Biosafety level 3 facility Message-ID: <6.0.0.22.1.20070614105206.01b07670@gemini.msu.montana.edu> We are looking for a biocontainer or a box (may be clear plastic of some type) with a handle and lock to be used for carrying vials in and out of a Biosafely Level 3 facility. The only one we have found is polycarbonate which means you can't spray alcohol on this plastic, the alcohol eventually eats polycarbonate or damages it. So much for safety!! Any suggestions will be welcomed Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From liz <@t> premierlab.com Thu Jun 14 12:36:26 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Jun 14 12:36:36 2007 Subject: [Histonet] Biocontainers for carrying vials in and out of Biosafety level 3 facility In-Reply-To: <36ECAF7E60314457825B2118133E7892@PremierLab.local> References: <36ECAF7E60314457825B2118133E7892@PremierLab.local> Message-ID: Gayle Mopec has these containers called the pathports. We used them when we = were in the hospital. I'm not sure what kind of plastic they were made = of. They are orange tool boxes with handles and special seals. Here = is the link. http://www.mopec.com/chapter3/pdf/pathport.pdf Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com =20 Ship to Address: =20 Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu = [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle = Callis Sent: Thursday, June 14, 2007 11:16 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Biocontainers for carrying vials in and out of = Biosafety level 3 facility We are looking for a biocontainer or a box (may be clear plastic of some type) with a handle and lock to be used for carrying vials in and out of = a Biosafely Level 3 facility. The only one we have found is polycarbonate which means you can't spray = alcohol on this plastic, the alcohol eventually eats polycarbonate or = damages it. So much for safety!! Any suggestions will be welcomed Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG Free Edition.=20 Version: 7.5.472 / Virus Database: 269.8.15/848 - Release Date: = 6/13/2007 12:50 PM =20 No virus found in this outgoing message. Checked by AVG Free Edition.=20 Version: 7.5.472 / Virus Database: 269.8.15/848 - Release Date: = 6/13/2007 12:50 PM =20 From dellav <@t> musc.edu Thu Jun 14 14:43:11 2007 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Thu Jun 14 14:44:13 2007 Subject: [Histonet] Biocontainers for carrying vials in and out of Biosafety level 3 facility In-Reply-To: <6.0.0.22.1.20070614105206.01b07670@gemini.msu.montana.edu> References: <6.0.0.22.1.20070614105206.01b07670@gemini.msu.montana.edu> Message-ID: <7F6B678A32B0564196138E6B3101996A5E2474@EVS1.clinlan.local> Gayle, You may find something you can use in a Lowes or Home Depot. Look for polyethylene (or is it propylene) toolbox. We did this at a prior facility where we purchased red plastic toolboxes. Sometimes you can find them in yellow or red which kind of goes along with the biohazard theme. Depending upon what you are transporting you can probably get something as cheap as $10, especially if color or transparency is not an issue. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Thursday, June 14, 2007 12:56 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Biocontainers for carrying vials in and out of Biosafety level 3 facility We are looking for a biocontainer or a box (may be clear plastic of some type) with a handle and lock to be used for carrying vials in and out of a Biosafely Level 3 facility. The only one we have found is polycarbonate which means you can't spray alcohol on this plastic, the alcohol eventually eats polycarbonate or damages it. So much for safety!! Any suggestions will be welcomed Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kim.Shields <@t> viha.ca Thu Jun 14 15:40:11 2007 From: Kim.Shields <@t> viha.ca (Shields, Kim) Date: Thu Jun 14 15:40:18 2007 Subject: [Histonet] automated microtome recommendations Message-ID: We are in the market for new automated microtomes. Any recommendations on makes & models? They need to be real workhorses!! Also, has anyone used a microtome that faces the block automatically? We understand it's possible, but don't know who makes them, & if they really can do it. Thanks in advance, we really look forward to your recommendations. Kim Shields VIHA Victoria, BC Canada From MBURTON1 <@t> PARTNERS.ORG Thu Jun 14 16:07:21 2007 From: MBURTON1 <@t> PARTNERS.ORG (Burton, Mark) Date: Thu Jun 14 16:03:59 2007 Subject: [Histonet] Digital Camera Message-ID: Hello, We are looking for a high-end digital camera for documentation and publishing. Has anyone had experience with the Olympus Q-Color cameras and/or related software? Can anyone recommend a comparable camera in the $3-$5k range? Thank you. Mark Burton HTL ASCP Brigham and Women's Hospital Boston, MA The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From liz <@t> premierlab.com Thu Jun 14 16:05:15 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Jun 14 16:05:25 2007 Subject: [Histonet] automated microtome recommendations In-Reply-To: <35AE7D22AC254701BCF2CD087A0584DF@PremierLab.local> References: <35AE7D22AC254701BCF2CD087A0584DF@PremierLab.local> Message-ID: Kim I like the Leica RM2255 that=92s what we have. We also looked at the = Micron and thought that was a nice unit also. The decision came down to = price, we got a better price for the Leica RM2255 than with the Micron. Liz=20 Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com =20 Ship to Address: =20 Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu = [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shields, = Kim Sent: Thursday, June 14, 2007 3:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] automated microtome recommendations We are in the market for new automated microtomes. Any recommendations on makes & models? They need to be real = workhorses!! Also, has anyone used a microtome that faces the block automatically? We = understand it's possible, but don't know who makes them, & if they = really can do it. Thanks in advance, we really look forward to your recommendations. Kim Shields VIHA Victoria, BC Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG Free Edition.=20 Version: 7.5.472 / Virus Database: 269.8.15/848 - Release Date: = 6/13/2007 12:50 PM =20 No virus found in this outgoing message. Checked by AVG Free Edition.=20 Version: 7.5.472 / Virus Database: 269.8.15/848 - Release Date: = 6/13/2007 12:50 PM =20 From RODOCKER_KERRY_B <@t> LILLY.COM Fri Jun 15 06:59:47 2007 From: RODOCKER_KERRY_B <@t> LILLY.COM (Kerry B Rodocker) Date: Fri Jun 15 06:59:59 2007 Subject: [Histonet] Receptor IHC Message-ID: Hello: Does anyone have experience with performing IHC for receptors such as Prolactin Receptor and Growth Hormone Receptor? We would be looking at mouse and rat tissues. Thanks Kerry Rodocker Eli Lilly and Company Greenfield IN 46140 From tkngflght <@t> yahoo.com Fri Jun 15 07:15:27 2007 From: tkngflght <@t> yahoo.com (Cheryl) Date: Fri Jun 15 07:15:32 2007 Subject: [Histonet] In need of a few more temp techs!! In-Reply-To: Message-ID: <476955.23570.qm@web50901.mail.re2.yahoo.com> Good morning! We're in need of a few more great techs who are (or want to be) travelers. Give us a call if interested--working to place folks from early next week through the beginning of July right now with others coming open through the summer. THANKS!! Cheryl Kerry, HT(ASCP) Full Staff Inc. 281.852.9457 800.756.3309 PS. If we've already talked--please don't hesitate to touch base if you're ready to go! From rjbuesa <@t> yahoo.com Fri Jun 15 07:38:10 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jun 15 07:38:15 2007 Subject: [Histonet] automated microtome recommendations In-Reply-To: Message-ID: <185199.64083.qm@web61224.mail.yahoo.com> Ask you Leica representative, they manufacture automated microtomer that face off (in the "thick" mode) the blocks, before starting in the "thin" mode. They are really very good. Ren? J. "Shields, Kim" wrote: We are in the market for new automated microtomes. Any recommendations on makes & models? They need to be real workhorses!! Also, has anyone used a microtome that faces the block automatically? We understand it's possible, but don't know who makes them, & if they really can do it. Thanks in advance, we really look forward to your recommendations. Kim Shields VIHA Victoria, BC Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ready for the edge of your seat? Check out tonight's top picks on Yahoo! TV. From mcauliff <@t> umdnj.edu Fri Jun 15 08:45:29 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Jun 15 08:45:51 2007 Subject: [Histonet] TH staining In-Reply-To: <000601c7ae3c$658e16b0$5e284684@natali> References: <000601c7ae3c$658e16b0$5e284684@natali> Message-ID: <467297F9.4040602@umdnj.edu> Hi Natalie: I use anti-TH from Pel-Freez in Rogers, Arkansas along with a Vector ABC Elite kit. It is relatively easy to get good TH staining in CNS or adrenal medulla. Are you using fixed, frozen sections or wax embedded? Is your peroxide fresh (< 1 year old)? How long is the tissue fixed and in what fixative? Geoff Natalie Nagorsky wrote: > Hi Histonet, > > > > I have several protocols for Tirosine Hydroxylase and I was not successful > to get good results. > > May some one share the working protocol and sours for TH. > > > > Any help will be appreciate. > > Natalie > > Brain Research Center > > Bar-Ilan University > > Israel > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From jflinn <@t> gmu.edu Fri Jun 15 08:58:45 2007 From: jflinn <@t> gmu.edu (Jane M Flinn) Date: Fri Jun 15 08:58:55 2007 Subject: [Histonet] automated microtome recommendations In-Reply-To: <185199.64083.qm@web61224.mail.yahoo.com> References: <185199.64083.qm@web61224.mail.yahoo.com> Message-ID: What thoughts do people have on the relative advantages of high and low profile knives for slicing mouse or rat brain? We use fresh frozen tissue. jane "Life is short - make haste to be kind" Dr. Jane Flinn Director, Biopsychology Program George Mason University, 3F5 4400 University Dr. Fairfax, VA 22030 Phone: 703-993-4107 Fax: 703-993-1359 ----- Original Message ----- From: Rene J Buesa Date: Friday, June 15, 2007 8:38 am Subject: Re: [Histonet] automated microtome recommendations > Ask you Leica representative, they manufacture automated > microtomer that face off (in the "thick" mode) the blocks, before > starting in the "thin" mode. > They are really very good. > Ren? J. > > "Shields, Kim" wrote: > We are in the market for new automated microtomes. > > Any recommendations on makes & models? They need to be real > workhorses!! > Also, has anyone used a microtome that faces the block > automatically? We understand it's possible, but don't know who > makes them, & if they really can do it. > > Thanks in advance, we really look forward to your recommendations. > > Kim Shields > VIHA > Victoria, BC > Canada > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Ready for the edge of your seat? Check out tonight's top picks on > Yahoo! TV. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From vazquezr <@t> ohsu.edu Fri Jun 15 09:07:40 2007 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Jun 15 09:08:04 2007 Subject: [Histonet] -80 freezer Message-ID: Cindi, I have a Thermo Electron Forma -86 ULT freezer. It sits behind me in the lab and I don't even know that it is there. Very quiet and no kit needed to be installed. Robyn OHSU >>> "Sharon Bledsoe" 6/13/2007 1:31 PM >>> Cindi, Yes, I do indeed, have an opinion. We recently bought a Revco 24.4 cu ft Ultra II, it was so loud that we had to buy a sound abatement kit and had to have someone from the company come to install it. It's nice, works great, but it is LOUD. Sharon At 2:19 PM -0500 6/13/07, cindipqr@wowway.com wrote: >Hi All, >I'm in the market for a -80 freezer. Does anyone have any opinions- pro or >con? >Thanks, >Cindi >HFH >Detroit, Mi > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Jun 15 09:27:18 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jun 15 09:27:22 2007 Subject: [Histonet] automated microtome recommendations In-Reply-To: Message-ID: <382134.24506.qm@web61221.mail.yahoo.com> Any "tough subject" requires high profile as a better alternative. Ren? J. Jane M Flinn wrote: What thoughts do people have on the relative advantages of high and low profile knives for slicing mouse or rat brain? We use fresh frozen tissue. jane "Life is short - make haste to be kind" Dr. Jane Flinn Director, Biopsychology Program George Mason University, 3F5 4400 University Dr. Fairfax, VA 22030 Phone: 703-993-4107 Fax: 703-993-1359 ----- Original Message ----- From: Rene J Buesa Date: Friday, June 15, 2007 8:38 am Subject: Re: [Histonet] automated microtome recommendations > Ask you Leica representative, they manufacture automated > microtomer that face off (in the "thick" mode) the blocks, before > starting in the "thin" mode. > They are really very good. > Ren??? J. > > "Shields, Kim" wrote: > We are in the market for new automated microtomes. > > Any recommendations on makes & models? They need to be real > workhorses!! > Also, has anyone used a microtome that faces the block > automatically? We understand it's possible, but don't know who > makes them, & if they really can do it. > > Thanks in advance, we really look forward to your recommendations. > > Kim Shields > VIHA > Victoria, BC > Canada > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Ready for the edge of your seat? Check out tonight's top picks on > Yahoo! TV. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > begin:vcard n:Flinn;Jane M fn:Jane M Flinn tel;fax:703-993-1359 tel;work:703-993-1353 org:George Mason University;Psychology, 3F5 adr:;;4400 University Dr.;Fairfax;VA;22030; version:2.1 email;internet:jflinn@gmu.edu title:Director, Biopsychology Program end:vcard --------------------------------- Never miss an email again! Yahoo! Toolbar alerts you the instant new Mail arrives. Check it out. From andrae <@t> u.washington.edu Fri Jun 15 10:21:44 2007 From: andrae <@t> u.washington.edu (A. Erickson) Date: Fri Jun 15 10:21:50 2007 Subject: [Histonet] TH staining In-Reply-To: <467297F9.4040602@umdnj.edu> References: <000601c7ae3c$658e16b0$5e284684@natali> <467297F9.4040602@umdnj.edu> Message-ID: Geoff- would you be willing to post information to Histonet regarding xTH antibody? Especially order info? Thanks, andra On Fri, 15 Jun 2007, Geoff McAuliffe wrote: > Hi Natalie: > > I use anti-TH from Pel-Freez in Rogers, Arkansas along with a Vector ABC > Elite kit. It is relatively easy to get good TH staining in CNS or adrenal > medulla. > Are you using fixed, frozen sections or wax embedded? > Is your peroxide fresh (< 1 year old)? > How long is the tissue fixed and in what fixative? > > Geoff > > Natalie Nagorsky wrote: >> Hi Histonet, >> >> >> I have several protocols for Tirosine Hydroxylase and I was not successful >> to get good results. >> >> May some one share the working protocol and sours for TH. >> >> >> Any help will be appreciate. >> >> Natalie >> >> Brain Research Center >> >> Bar-Ilan University >> >> Israel >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > > > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583 mcauliff@umdnj.edu > ********************************************** > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gcallis <@t> montana.edu Fri Jun 15 11:10:33 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Jun 15 11:10:03 2007 Subject: [Histonet] automated microtome recommendations In-Reply-To: References: <185199.64083.qm@web61224.mail.yahoo.com> Message-ID: <6.0.0.22.1.20070615095634.01b4e1e0@gemini.msu.montana.edu> Jane, We use high profile disposable blades, Sakura FineTek Accuedge coated blades for all our cryomicrotomy. The high profile is a bit sturdier than low profile and is JUST as sharp as low profile. To satisfy curiousity about any differences of these blades, the thickness of both was measured with a dial caliper, and learned high profiles are just a fraction thicker below the slanted bevel edge than the low profile. We do not experience chatter, vibration with the high profile blades, but have had some problems with low profiles. Be sure to try the different blades available, ask for samples - ThermoFisher, DuraEdge, and Richard Allan EdgeRite are excellent high profiles too. There are more besides these. What is critical is the temperature you set for the brain cryotomy - we cryosection at -16C, and some advise lower temps than that, but this temperature works for brain, spleen and liver. Our cryostat does not have a separate temperature control for sample or blade. At 07:58 AM 6/15/2007, you wrote: >What thoughts do people have on the relative advantages of high and low >profile knives for slicing mouse or rat brain? We use fresh frozen tissue. jane > >"Life is short - make haste to be kind" > >Dr. Jane Flinn >Director, Biopsychology Program >George Mason University, 3F5 >4400 University Dr. >Fairfax, VA 22030 >Phone: 703-993-4107 >Fax: 703-993-1359 Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From Robert.Lott <@t> TriadHospitals.com Fri Jun 15 11:37:14 2007 From: Robert.Lott <@t> TriadHospitals.com (Lott, Robert) Date: Fri Jun 15 11:37:24 2007 Subject: [Histonet] Fixation for Her-2/neu -Where's the Beef??? Message-ID: <673832E27C45FC4D97EF758FFC777C27021AB54B@CPRTEVS01.triadhospitals.net> Hello Everyone, .....been a lot of talk about this already, I'm aware of that. Now I want to know if there is any "real" scientific" basis for the new CAP guideline for Her-2 IHC fixation that states "No less than 6 hrs. and no more than 48 hrs. in aqueous NFB." Any documented, peer-reviewed, published data??? My sense is that this guideline really does represent best practice. Most of us do well to get our breast specimens fixed for 6 hrs. in NBF!! However, is there really some difference in performance of the Her-2 IHC assay if a breast case is fixed for 48 hrs vs. one fixed for 54 hrs. or 72 hrs. (i.e. weekend/ holiday scenario). UNDER-fixation would seem to be so much more of issue than OVER-fixation. It's this top end that bothers me so much!!! I am not aware of real data to support this new guideline! Does anyone out there know anything different????? Robert Robert L. Lott, HTL(ASCP) Manager, Anatomic Pathology LabFirst / Trinity Medical Center - formerly Montclair Baptist Medical Center 800 Montclair Road Birmingham, AL 35213 205-592-5388 phone 205-592-5646 fax robert.lott@triadhospitals.com From mcauliff <@t> umdnj.edu Fri Jun 15 12:18:08 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Jun 15 12:18:29 2007 Subject: [Histonet] TH staining In-Reply-To: References: <000601c7ae3c$658e16b0$5e284684@natali> <467297F9.4040602@umdnj.edu> Message-ID: <4672C9D0.3090806@umdnj.edu> Sure: Pel-Freez Biologicals P.O. box 68 205 North Arkansas Rogers, Ark. 72757 800-643-3426 As I mentioned in my last post, I use the Vector ABC Elite kit which is much more sensitive than the ordinary ABC kit (but the latter works fine). Using the Elite kit we were able to get a good rxn. on FFPE adrenal glands. Also, for TH fixation with the low pH- high pH sequence reported by Berod (J. Histochem. Cytochem. 29:844-850 1981) gives better results, especially for fine nerve fibers in the cortex, than routine buffered formaldehyde. Geoff A. Erickson wrote: > Geoff- would you be willing to post information to Histonet regarding > xTH antibody? Especially order info? Thanks, andra > > On Fri, 15 Jun 2007, Geoff McAuliffe wrote: > >> Hi Natalie: >> >> I use anti-TH from Pel-Freez in Rogers, Arkansas along with a >> Vector ABC Elite kit. It is relatively easy to get good TH staining >> in CNS or adrenal medulla. >> Are you using fixed, frozen sections or wax embedded? >> Is your peroxide fresh (< 1 year old)? >> How long is the tissue fixed and in what fixative? >> >> Geoff >> >> Natalie Nagorsky wrote: >>> Hi Histonet, >>> >>> >>> I have several protocols for Tirosine Hydroxylase and I was not >>> successful >>> to get good results. >>> >>> May some one share the working protocol and sours for TH. >>> >>> >>> Any help will be appreciate. >>> >>> Natalie >>> >>> Brain Research Center >>> >>> Bar-Ilan University >>> >>> Israel >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >>> >> >> >> -- >> -- >> ********************************************** >> Geoff McAuliffe, Ph.D. >> Neuroscience and Cell Biology >> Robert Wood Johnson Medical School >> 675 Hoes Lane, Piscataway, NJ 08854 >> voice: (732)-235-4583 mcauliff@umdnj.edu >> ********************************************** >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From bjdewe <@t> aol.com Fri Jun 15 14:04:20 2007 From: bjdewe <@t> aol.com (bjdewe@aol.com) Date: Fri Jun 15 14:04:28 2007 Subject: [Histonet] Hard Antibodies! Message-ID: <8C97D971CE35C2A-554-49E1@webmail-dd03.sysops.aol.com> Does anyone have any antibodies they consider especially hard to do? I'm curious because I know what we have trouble with in our lab but am curious if anyone else has the same issues... Thanks in advance for your input! Cheers, Lorie Live as if you were to die tomorrow. Learn as if you were to live forever. - Gandhi - ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From talulahgosh <@t> gmail.com Fri Jun 15 14:11:36 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Jun 15 14:11:41 2007 Subject: [Histonet] Hard Antibodies! In-Reply-To: <8C97D971CE35C2A-554-49E1@webmail-dd03.sysops.aol.com> References: <8C97D971CE35C2A-554-49E1@webmail-dd03.sysops.aol.com> Message-ID: rabbit anti-chick Hox D11, definitely!! rabbit anti-chick RALDH-2 also these are not made by companies though, so that could be why. we've also tried antibodies from Chemicon (now Millipore)--rabbit anti-LIM 1 and rabbit anti-neurofilament--and have had trouble with background, even after trouble-shooting the dilution and fixation time. Emily -- penguins, spoons, and you--what's life like among the flightless? http://www.getafirstlife.com/ From rjbuesa <@t> yahoo.com Fri Jun 15 14:38:22 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jun 15 14:38:25 2007 Subject: [Histonet] Hard Antibodies! In-Reply-To: <8C97D971CE35C2A-554-49E1@webmail-dd03.sysops.aol.com> Message-ID: <508746.57185.qm@web61225.mail.yahoo.com> Alk-1; BCL-6; CD4; and EGFR Ren? J. --------------------------------- Sick sense of humor? Visit Yahoo! TV's Comedy with an Edge to see what's on, when. From rjbuesa <@t> yahoo.com Fri Jun 15 14:43:40 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jun 15 14:43:44 2007 Subject: [Histonet] Fixation for Her-2/neu -Where's the Beef??? In-Reply-To: <673832E27C45FC4D97EF758FFC777C27021AB54B@CPRTEVS01.triadhospitals.net> Message-ID: <329825.27255.qm@web61223.mail.yahoo.com> Before this regulation came to be we never left breast to fix for less than 12 hours and weekend specimens could be in NBF for up to 48 hours. There were even cases when we "re-sampled" the breast after several days in NBF and these "re-sampled" pieces gave the same results as the first time. Probably this CAP regulation is intended to standardize the procedure. I do not know of any peer reviewed articles recommending these fixation times (although I may be wrong as in other occasions). Ren? J. --------------------------------- Pinpoint customers who are looking for what you sell. From burch007 <@t> mc.duke.edu Fri Jun 15 15:12:45 2007 From: burch007 <@t> mc.duke.edu (James L Burchette) Date: Fri Jun 15 15:13:01 2007 Subject: [Histonet] Hard Antibodies! In-Reply-To: <8C97D971CE35C2A-554-49E1@webmail-dd03.sysops.aol.com> Message-ID: CD133!!!! Jim Burchette, HT(ASCP) QIHC "A simple histotech from a little country hospital in North Carolina" bjdewe@aol.com Sent by: histonet-bounces@lists.utsouthwestern.edu 06/15/2007 02:04 PM To Histonet@lists.utsouthwestern.edu cc Subject [Histonet] Hard Antibodies! Does anyone have any antibodies they consider especially hard to do? I'm curious because I know what we have trouble with in our lab but am curious if anyone else has the same issues... Thanks in advance for your input! Cheers, Lorie Live as if you were to die tomorrow. Learn as if you were to live forever. - Gandhi - ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Fri Jun 15 15:23:39 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Jun 15 15:23:08 2007 Subject: [Histonet] ImmPACT DAB Message-ID: <6.0.0.22.1.20070615141643.01b20aa0@gemini.msu.montana.edu> Dear all, I am curious to know how people are liking the new ImmPACT DAB from Vector? The idea of a stable working solutions for days and/or weeks depending on storage conditions along with the simplicity of a two reagent system - DAB stock and diluent, is very appealing to those who do manual staining too. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From TJasper <@t> smdc.org Fri Jun 15 15:32:10 2007 From: TJasper <@t> smdc.org (Jasper, Thomas G.) Date: Fri Jun 15 15:32:33 2007 Subject: [Histonet] Fixation for Her-2/neu -Where's the Beef??? In-Reply-To: <673832E27C45FC4D97EF758FFC777C27021AB54B@CPRTEVS01.triadhospitals.net> Message-ID: <1A9F2A6C5762524799A816F1F09744CF0143A5DA@SCREECH.ntcampus.smdc.org> Hey Robert, I've been informed from our pathologists that over-fixation of breast tissue (more than 48 hours) will potentially mask Her2 staining. This in turn impacts our FISH staining, as cases for FISH are selected based on Her2 results. The fear then, is that cases which should've reflexed to FISH will not. The over-fixation could result in under-expression of Her2 run cases. Sounds a bit contradictory but that's how I understand it. I do have to agree with you, my concern would be under-fixation vs. over-fixation. But, I guess under-fixation is more readily apparent and easier to address than over-fixation. This will be a new thing for everybody and we're looking at a new mandatory week-end shift because of it (oh joy). Anyway, this is what I've been told and how I understand it, good luck down there in Bama. Tom Jasper HT (ASCP) BAS SMDC Duluth, MN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Lott, Robert Sent: Friday, June 15, 2007 11:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fixation for Her-2/neu -Where's the Beef??? Hello Everyone, .....been a lot of talk about this already, I'm aware of that. Now I want to know if there is any "real" scientific" basis for the new CAP guideline for Her-2 IHC fixation that states "No less than 6 hrs. and no more than 48 hrs. in aqueous NFB." Any documented, peer-reviewed, published data??? My sense is that this guideline really does represent best practice. Most of us do well to get our breast specimens fixed for 6 hrs. in NBF!! However, is there really some difference in performance of the Her-2 IHC assay if a breast case is fixed for 48 hrs vs. one fixed for 54 hrs. or 72 hrs. (i.e. weekend/ holiday scenario). UNDER-fixation would seem to be so much more of issue than OVER-fixation. It's this top end that bothers me so much!!! I am not aware of real data to support this new guideline! Does anyone out there know anything different????? Robert Robert L. Lott, HTL(ASCP) Manager, Anatomic Pathology LabFirst / Trinity Medical Center - formerly Montclair Baptist Medical Center 800 Montclair Road Birmingham, AL 35213 205-592-5388 phone 205-592-5646 fax robert.lott@triadhospitals.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From Wanda.Smith <@t> HCAhealthcare.com Fri Jun 15 16:02:10 2007 From: Wanda.Smith <@t> HCAhealthcare.com (Smith Wanda) Date: Fri Jun 15 16:02:18 2007 Subject: [Histonet] unsubscribe Message-ID: <817C2761C5A1394180709AEEDB775B7E02B09137@NASEV03.hca.corpad.net> Wanda G. Smith, HTL(ASCP)HT Trident Medical Center Pathology Supervisor ph: 843-847-4586 fx: 843-847-4296 email: wanda.smith@hcahealthcare.com From lscott <@t> sfcn.org Fri Jun 15 16:09:51 2007 From: lscott <@t> sfcn.org (Scott) Date: Fri Jun 15 16:10:09 2007 Subject: [Histonet] Sakura E300 Retort Question Message-ID: <000c01c7af91$836f80f0$0400a8c0@computer> Does anyone use an Sakura E300 tissue processor ? Our lab has one and the top edge of the retort (where you put the specimens to process) is hot in one spot. The left side on top,where an air vent hole is. This is after the process is completed,and the cleaning cycle is finished. I have never noticed this before. I spoke with a Sakura rep. and they didn't seem to know if this is normal. If anyone has an E300 would you please check to see if the retort is hot (in this one spot only) when the processor is just waiting for the next run? Thanks, Scott Hendricksen HT(ASCP) From dellav <@t> musc.edu Fri Jun 15 16:25:46 2007 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Fri Jun 15 16:27:05 2007 Subject: [Histonet] Fixation for Her-2/neu -Where's the Beef??? In-Reply-To: <673832E27C45FC4D97EF758FFC777C27021AB54B@CPRTEVS01.triadhospitals.net> References: <673832E27C45FC4D97EF758FFC777C27021AB54B@CPRTEVS01.triadhospitals.net> Message-ID: <7F6B678A32B0564196138E6B3101996A63084E@EVS1.clinlan.local> Robert, I'm not sure this answers your question but I believe the CAP "guidelines" are based largely upon the article published by Neal Goldstein et. al. entitled " Minimum Formalin Fixation Time for Consistent Estrogen Receptor Immunohistochemical Staining of Invasive Breast Carcinoma" Neal S. Goldstein, MD, Monica Ferkowicz, MT(ASCP), PathA(AAPA), Eva Odish, HTL(IHQ), Anju Mani, MD, and Farnaz Hastah, MD Am J Clin Pathol 2003;120:86-92 This work likely led to the ASCO/CAP publication upon which the current requirements are based "American Society of Clinical Oncology/College of American Pathologists Guideline Recommendations for Human Epidermal Growth Factor Receptor 2 Testing in Breast Cancer" Arch Pathol Lab Med-Vol 131, January 2007 I have both in pdf for anyone who can't get their hands on these publications and would like to email me. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lott, Robert Sent: Friday, June 15, 2007 12:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fixation for Her-2/neu -Where's the Beef??? Hello Everyone, .....been a lot of talk about this already, I'm aware of that. Now I want to know if there is any "real" scientific" basis for the new CAP guideline for Her-2 IHC fixation that states "No less than 6 hrs. and no more than 48 hrs. in aqueous NFB." Any documented, peer-reviewed, published data??? My sense is that this guideline really does represent best practice. Most of us do well to get our breast specimens fixed for 6 hrs. in NBF!! However, is there really some difference in performance of the Her-2 IHC assay if a breast case is fixed for 48 hrs vs. one fixed for 54 hrs. or 72 hrs. (i.e. weekend/ holiday scenario). UNDER-fixation would seem to be so much more of issue than OVER-fixation. It's this top end that bothers me so much!!! I am not aware of real data to support this new guideline! Does anyone out there know anything different????? Robert Robert L. Lott, HTL(ASCP) Manager, Anatomic Pathology LabFirst / Trinity Medical Center - formerly Montclair Baptist Medical Center 800 Montclair Road Birmingham, AL 35213 205-592-5388 phone 205-592-5646 fax robert.lott@triadhospitals.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Sat Jun 16 03:19:13 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Sat Jun 16 03:19:19 2007 Subject: [Histonet] Hard Antibodies! In-Reply-To: References: <8C97D971CE35C2A-554-49E1@webmail-dd03.sysops.aol.com> Message-ID: regarding your hard-to-process antibodies--are they manufactured by a company, or has an individual made them? does this make a difference? in response to my own question, the antibodies we receive from Developmental Studies Hybridoma Bank (DSHB, University of Iowa), Molecular Probes, and Rockland Immuno work very well. our problem is with antibodies from individual labs. could this be a problem with shipping? the antibodies we receive from individual labs are not on dry ice, but a PI told me that since human antibodies can survive at high temperatures, shipped antibodies can survive it as well.* Emily *all of this does not apply to Chemicon antibodies, which suck. -- penguins, spoons, and you--what's life like among the flightless? http://www.getafirstlife.com/ From talulahgosh <@t> gmail.com Sat Jun 16 03:34:02 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Sat Jun 16 03:34:07 2007 Subject: [Histonet] other lists, in addition Message-ID: though i thoroughly appreciate the guidance i find on this list, i was wondering if another list exists specifically for technicians in developmental biology. i have googled what i thought was the correct terms, but came up with nothing. is it not common for email lists of the histonet variety to exist? Emily -- penguins, spoons, and you--what's life like among the flightless? http://www.getafirstlife.com/ From gu.lang <@t> gmx.at Sat Jun 16 07:51:03 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Jun 16 07:51:11 2007 Subject: AW: [Histonet] Fixation for Her-2/neu -Where's the Beef??? In-Reply-To: <673832E27C45FC4D97EF758FFC777C27021AB54B@CPRTEVS01.triadhospitals.net> Message-ID: <001101c7b014$ffe32820$6412a8c0@dielangs.at> On a recent workshop a speaker of NordiQC gave information on their sampled knowledge on this subject. He said, that the receptor-results are most influenced by underfixation. Especially in the comparison of small needle-biopsies to the large-mamma-surgical specimen he said, that there is no difference in the IHC after a minimum fixation of 6 hours. - I think, that is the usual problem with the rush-biopsies, that show differences in the margins and the center; and afterwards to the big speciman. Overfixation should'nt be a problem up to some weeks. And in the clinical histolab the usually used markers all work well with a fixation duration of 5-6 days. So no worries about too long fixation. Some markers like lambda or kappa react sensitive on underfixation and give either false positive or false negative results. If somebody is interested in this organisation for Quality Control in IHC: http://www.nordiqc.org Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Lott, Robert Gesendet: Freitag, 15. Juni 2007 18:37 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Fixation for Her-2/neu -Where's the Beef??? Hello Everyone, .....been a lot of talk about this already, I'm aware of that. Now I want to know if there is any "real" scientific" basis for the new CAP guideline for Her-2 IHC fixation that states "No less than 6 hrs. and no more than 48 hrs. in aqueous NFB." Any documented, peer-reviewed, published data??? My sense is that this guideline really does represent best practice. Most of us do well to get our breast specimens fixed for 6 hrs. in NBF!! However, is there really some difference in performance of the Her-2 IHC assay if a breast case is fixed for 48 hrs vs. one fixed for 54 hrs. or 72 hrs. (i.e. weekend/ holiday scenario). UNDER-fixation would seem to be so much more of issue than OVER-fixation. It's this top end that bothers me so much!!! I am not aware of real data to support this new guideline! Does anyone out there know anything different????? Robert Robert L. Lott, HTL(ASCP) Manager, Anatomic Pathology LabFirst / Trinity Medical Center - formerly Montclair Baptist Medical Center 800 Montclair Road Birmingham, AL 35213 205-592-5388 phone 205-592-5646 fax robert.lott@triadhospitals.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cindipqr <@t> wowway.com Sat Jun 16 11:00:29 2007 From: cindipqr <@t> wowway.com (cindipqr@wowway.com) Date: Sat Jun 16 11:00:36 2007 Subject: [Histonet] Hard Antibodies! In-Reply-To: References: <8C97D971CE35C2A-554-49E1@webmail-dd03.sysops.aol.com> Message-ID: <20070616155900.M92025@wowway.com> Now that you brought it up, has anybody been having problems with Chemicon's MAB1281 and MAB1273? (human nuclei and human mitochondria) ---------- Original Message ----------- From: "Emily Sours" To: "James L Burchette" Cc: Histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Sent: Sat, 16 Jun 2007 04:19:13 -0400 Subject: Re: [Histonet] Hard Antibodies! > regarding your hard-to-process antibodies--are they manufactured by a > company, or has an individual made them? does this make a difference? > in response to my own question, the antibodies we receive from > Developmental Studies Hybridoma Bank (DSHB, University of Iowa), > Molecular Probes, and Rockland Immuno work very well. our problem is > with antibodies from individual labs. > could this be a problem with shipping? the antibodies we receive from > individual labs are not on dry ice, but a PI told me that since human > antibodies can survive at high temperatures, shipped antibodies can > survive it as well.* > > Emily > *all of this does not apply to Chemicon antibodies, which suck. > -- > penguins, spoons, and you--what's life like among the flightless? > http://www.getafirstlife.com/ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------- End of Original Message ------- From liz <@t> premierlab.com Sat Jun 16 11:22:51 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Sat Jun 16 11:23:09 2007 Subject: [Histonet] Hard Antibodies! - long response In-Reply-To: <7501EF4B983F46CDBC8FDC90D09BE203@PremierLab.local> References: <8C97D971CE35C2A-554-49E1@webmail-dd03.sysops.aol.com> <7501EF4B983F46CDBC8FDC90D09BE203@PremierLab.local> Message-ID: Emily When you purchase antibodies from vendors they "hopefully" have gone = through a process in which the antibody has been purified, this = information is on the specification sheet under antibody form, there are = several forms of antibodies, such as: Affinity Purified IgG Stabilized Antisera Ascites Fluid Cell Culture Supernatant Whole Antiserum IgG Fraction=20 Most vendors will tell you what the antibody form is and what the = purification method was. Purification is the removal of unwanted = immunoglobulins or IgG's that do not react with the protein of interest. = Vendors should also determine the specificity and sensitivity of the = antibody which can be done through a series of immunochemical techniques = including but not limited to immunoelectrophoresis, westerns blots, = double diffusion, rocket immunoelectrophoresis and ELISA, this = information is also sometimes listed on the specification sheet. If not = you can always call the vendor. In addition to these techniques they = could also test the antibody on a wide range of positive and negative = tissues with different staining protocols. But ultimately it=92s the = user=92s responsibility to ensure the quality of the primary antibody = prior to use, this is what we do with our titers studies and validation = protocols. =20 Antibodies that are so called "in house" antibodies are or are not = purified. Unpurified antibodies can consist of a wide variety of = different immunoglobulins some that are directed against the protein of = interest and some that are not. It is very difficult to deal with these = types of unpurified antibodies. I have not had the opportunity to work = with many, but overall I would have to say that they don't work that = well. One thing that needs to be considered is what is the protein = concentration of the antibody of interest? How can you determine that = when you have other IgG's in the mix, if you do not know the protein = concentration or form of your antibody how can you possibly set up the = appropriate negative control? When getting an "in house" antibody in = for evaluation I would definitely ask the following questions: Has it = been purifed? what form is it? Whats the protein concentration? And if = any testing has been performed regarding to specificity?=20 Regardless of whether you are dealing with a commerically available = antibody or an "in house" preparation careful review of the = specification sheet along with current literature is necessary. You = need to know as much as possible about what you are working with inorder = to be able to evalute the results of the titer studies. =20 Just my two cents, whatever its worth. I'm stepping off the soap box = now. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com =20 Ship to Address: =20 Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu = [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily = Sours Sent: Saturday, June 16, 2007 2:46 AM To: James L Burchette Cc: Histonet@lists.utsouthwestern.edu; = histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Hard Antibodies! regarding your hard-to-process antibodies--are they manufactured by a = company, or has an individual made them? does this make a difference? in response to my own question, the antibodies we receive from = Developmental Studies Hybridoma Bank (DSHB, University of Iowa), = Molecular Probes, and Rockland Immuno work very well. our problem is = with antibodies from individual labs. could this be a problem with shipping? the antibodies we receive from = individual labs are not on dry ice, but a PI told me that since human = antibodies can survive at high temperatures, shipped antibodies can = survive it as well.* Emily *all of this does not apply to Chemicon antibodies, which suck. -- penguins, spoons, and you--what's life like among the flightless? http://www.getafirstlife.com/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG Free Edition.=20 Version: 7.5.472 / Virus Database: 269.8.17/850 - Release Date: = 6/15/2007 11:31 AM =20 No virus found in this outgoing message. Checked by AVG Free Edition.=20 Version: 7.5.472 / Virus Database: 269.8.17/850 - Release Date: = 6/15/2007 11:31 AM =20 From rchiovetti <@t> yahoo.com Sat Jun 16 13:50:28 2007 From: rchiovetti <@t> yahoo.com (Robert Chiovetti) Date: Sat Jun 16 13:50:32 2007 Subject: [Histonet] other lists, in addition Message-ID: <867604.7388.qm@web58911.mail.re1.yahoo.com> Hi Emily, I'm not sure exactly what you're looking for in a developmental biology group, but the Open Directory Project (dmoz) is a pretty good place to look. You might try, for example: http://dmoz.org/Science/Biology/Developmental_Biology/ Good luck! Bob Robert (Bob) Chiovetti, Ph.D. Southwest Precision Instruments www.swpinet.com Arizona's Microscopy Resource 132 North Elster Drive Tucson, AZ 85710-3212 Tel./Fax 520-546-4986 Member, Arizona Small Business Association (www.asba.com) ----- Original Message ---- From: Emily Sours To: histonet@lists.utsouthwestern.edu Sent: Saturday, June 16, 2007 1:34:02 AM Subject: [Histonet] other lists, in addition though i thoroughly appreciate the guidance i find on this list, i was wondering if another list exists specifically for technicians in developmental biology. i have googled what i thought was the correct terms, but came up with nothing. is it not common for email lists of the histonet variety to exist? Emily -- penguins, spoons, and you--what's life like among the flightless? http://www.getafirstlife.com/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Choose the right car based on your needs. Check out Yahoo! Autos new Car Finder tool. http://autos.yahoo.com/carfinder/ From ancillarypath <@t> mac.com Sat Jun 16 17:12:09 2007 From: ancillarypath <@t> mac.com (ancillarypath@mac.com) Date: Sat Jun 16 17:12:26 2007 Subject: [Histonet] HER2-related issues In-Reply-To: <200706161705.l5GH5Isi023387@mac.com> References: <200706161705.l5GH5Isi023387@mac.com> Message-ID: <3D1F4DF6-AABF-4531-AECE-B5A7DDE6F9E4@mac.com> Dear colleagues, I've been reading the postings on HER2-related issues. Allow me to address the fixation-related issues. 1. Overfixation: 48 versus 72 hours of fixation has no difference on the impact of protein or gene signal. The CAP guidelines will most likely be revised to increase the upper limit of cutoff to 72 hours. That said, overfixation seems to have some impact on hormone receptors, and given that every invasive breast cancer should be tested for both HER2 and hormone receptor, then people will need to address the upper limit of fixation time. 2. Undrefixation: This what really has had devastating effects on the quality of HER2 testing by IHC. The literature is there, but it's the usual, good-old literature published by Fox and others in the 80s and 90s. Everyone knows that formaldehyde penetrates the tissue at a very rapid rate of 1 mm/hour (more or less depending on fat content and other factors). But infiltration is NOT equal to fixation. The 6-hour minimum fixation requirement should be strictly adhered to. There is currently a loop hole in the minimum requirements for fixing core biopsies versus excision specimens. Cores are required to be fixed for a minimum of 1 hour. That will change to. The revised guidelines will most likely increase that to 6 hours. This is a very realistic and scientifically sound requirement. We wrote a document to help colleagues address various issues related to the impact on the guidelines on their lab. It can be found on our website (www.vitromolecular.com). Thanks, ================================ Hadi Yaziji, M.D., Medical Director Vitro Molecular Laboratories President, Ancillary Pathways 7000 62nd Avenue, PH-C Miami, FL 33143 T 305-740-4440 F. 786-513-0175 www.vitromolecular.com www.ancillarypath.com From scoop <@t> mail.nih.gov Sat Jun 16 18:15:59 2007 From: scoop <@t> mail.nih.gov (scoop@mail.nih.gov) Date: Sat Jun 16 18:16:05 2007 Subject: [Histonet] other lists, in addition Message-ID: You could try http://biowww.net/ There are many different user groups there. Good luck! From jnocito <@t> satx.rr.com Sat Jun 16 18:42:53 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Sat Jun 16 18:42:58 2007 Subject: [Histonet] Hard Antibodies! - long response References: <8C97D971CE35C2A-554-49E1@webmail-dd03.sysops.aol.com> <7501EF4B983F46CDBC8FDC90D09BE203@PremierLab.local> Message-ID: <00a901c7b070$0fc7da50$d49eae18@yourxhtr8hvc4p> I have one. Ooops! I'm sorry. I thought the subject line said "hard bodie= s"=20 Guess I should read the whole line. JTT ----- Original Message -----=20 From: "Liz Chlipala" To: "Emily Sours" ; "James L Burchette"=20 Cc: ;=20 Sent: Saturday, June 16, 2007 11:22 AM Subject: RE: [Histonet] Hard Antibodies! - long response Emily When you purchase antibodies from vendors they "hopefully" have gone thro= ugh=20 a process in which the antibody has been purified, this information is on= =20 the specification sheet under antibody form, there are several forms of=20 antibodies, such as: Affinity Purified IgG Stabilized Antisera Ascites Fluid Cell Culture Supernatant Whole Antiserum IgG Fraction Most vendors will tell you what the antibody form is and what the=20 purification method was. Purification is the removal of unwanted=20 immunoglobulins or IgG's that do not react with the protein of interest.=20 Vendors should also determine the specificity and sensitivity of the=20 antibody which can be done through a series of immunochemical techniques=20 including but not limited to immunoelectrophoresis, westerns blots, doubl= e=20 diffusion, rocket immunoelectrophoresis and ELISA, this information is al= so=20 sometimes listed on the specification sheet. If not you can always call = the=20 vendor. In addition to these techniques they could also test the antibod= y=20 on a wide range of positive and negative tissues with different staining=20 protocols. But ultimately it=92s the user=92s responsibility to ensure t= he=20 quality of the primary antibody prior to use, this is what we do with our= =20 titers studies and validation protocols. Antibodies that are so called "in house" antibodies are or are not purifi= ed.=20 Unpurified antibodies can consist of a wide variety of different=20 immunoglobulins some that are directed against the protein of interest an= d=20 some that are not. It is very difficult to deal with these types of=20 unpurified antibodies. I have not had the opportunity to work with many,= =20 but overall I would have to say that they don't work that well. One thin= g=20 that needs to be considered is what is the protein concentration of the=20 antibody of interest? How can you determine that when you have other IgG= 's=20 in the mix, if you do not know the protein concentration or form of your=20 antibody how can you possibly set up the appropriate negative control? W= hen=20 getting an "in house" antibody in for evaluation I would definitely ask t= he=20 following questions: Has it been purifed? what form is it? Whats the=20 protein concentration? And if any testing has been performed regarding to= =20 specificity? Regardless of whether you are dealing with a commerically available antib= ody=20 or an "in house" preparation careful review of the specification sheet al= ong=20 with current literature is necessary. You need to know as much as possib= le=20 about what you are working with inorder to be able to evalute the results= of=20 the titer studies. Just my two cents, whatever its worth. I'm stepping off the soap box now. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu=20 [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sou= rs Sent: Saturday, June 16, 2007 2:46 AM To: James L Burchette Cc: Histonet@lists.utsouthwestern.edu;=20 histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Hard Antibodies! regarding your hard-to-process antibodies--are they manufactured by a=20 company, or has an individual made them? does this make a difference? in response to my own question, the antibodies we receive from Developmen= tal=20 Studies Hybridoma Bank (DSHB, University of Iowa), Molecular Probes, and=20 Rockland Immuno work very well. our problem is with antibodies from=20 individual labs. could this be a problem with shipping? the antibodies we receive from=20 individual labs are not on dry ice, but a PI told me that since human=20 antibodies can survive at high temperatures, shipped antibodies can survi= ve=20 it as well.* Emily *all of this does not apply to Chemicon antibodies, which suck. -- penguins, spoons, and you--what's life like among the flightless? http://www.getafirstlife.com/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.5.472 / Virus Database: 269.8.17/850 - Release Date: 6/15/2007= =20 11:31 AM No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.5.472 / Virus Database: 269.8.17/850 - Release Date: 6/15/2007= =20 11:31 AM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet=20 From pruegg <@t> ihctech.net Sun Jun 17 12:33:50 2007 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Sun Jun 17 12:31:57 2007 Subject: [Histonet] ImmPACT DAB In-Reply-To: <6.0.0.22.1.20070615141643.01b20aa0@gemini.msu.montana.edu> Message-ID: <200706171731.l5HHVlbA012897@pro12.abac.com> I would be interested in this also. I have been using stable DAB from Open BioSystems (developed by Brigati) for years and really like the convenience of it, nothing to mix at all, you keep it in the freezer (-20) but it does not freeze (has methanol or glycerine in it), just pull it out and use as is. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Friday, June 15, 2007 2:24 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] ImmPACT DAB Dear all, I am curious to know how people are liking the new ImmPACT DAB from Vector? The idea of a stable working solutions for days and/or weeks depending on storage conditions along with the simplicity of a two reagent system - DAB stock and diluent, is very appealing to those who do manual staining too. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun Jun 17 12:36:26 2007 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Sun Jun 17 12:34:46 2007 Subject: [Histonet] Hard Antibodies! In-Reply-To: Message-ID: <200706171734.l5HHYMZp013790@pro12.abac.com> My antibody nemesis is CD31 on many species. Some of the phosopho antibodies are giving me big troubles as well. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James L Burchette Sent: Friday, June 15, 2007 2:13 PM To: bjdewe@aol.com Cc: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Hard Antibodies! CD133!!!! Jim Burchette, HT(ASCP) QIHC "A simple histotech from a little country hospital in North Carolina" bjdewe@aol.com Sent by: histonet-bounces@lists.utsouthwestern.edu 06/15/2007 02:04 PM To Histonet@lists.utsouthwestern.edu cc Subject [Histonet] Hard Antibodies! Does anyone have any antibodies they consider especially hard to do? I'm curious because I know what we have trouble with in our lab but am curious if anyone else has the same issues... Thanks in advance for your input! Cheers, Lorie Live as if you were to die tomorrow. Learn as if you were to live forever. - Gandhi - ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jlinda <@t> ces.clemson.edu Sun Jun 17 13:19:50 2007 From: jlinda <@t> ces.clemson.edu (Linda Jenkins) Date: Sun Jun 17 13:20:03 2007 Subject: [Histonet] RE: Technovit 7200VLC Message-ID: <6.2.3.4.2.20070617141122.03d3c078@mailhost.ces.clemson.edu> Andrew, Your recent post ask: "I am looking for other people that work with Technovit 7200VLC resin on large (25x25x4mm) samples. At the moment the infiltration takes 3 days for each of the graded stages (30-100%) and the final 100% resin step takes up to 18 days. This is what is recommended by the manufacturers of the Exakt system we use, but seems rather a long time! We have tried reducing the times but have ended up with poor section quality. Unfortunately we cannot reduce the sample size as serial sections have to be cut. Does anyone out there have any experience with this resin, and have a method to reduce the infiltration times? Any suggestions greatly appreciated." Thanks in advance, Andrew Andrew Prior Histologist Smith &Nephew Research Centre York Science Park Heslington York YO10 5DF UK I have a great deal of experience with this resin and, I agree, your procedure sounds too long;however, are you manually processing? Are you using vacuum and stirring? All these things help when processing samples. I will send you my procedure under separate cover. Good Luck, Linda Jenkins Clemson University Bioengineering Clemson, SC From AnthonyH <@t> chw.edu.au Sun Jun 17 17:53:34 2007 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Jun 17 17:53:51 2007 Subject: [Histonet] Fixation for Her-2/neu -Where's the Beef??? Message-ID: Some comments: Underfixed tissue retrieve poorly (looks like a bomb has hit the section). Overfixed tissues may require extended antigen retrieval, though usually not. It seems that there is no allowance for thickness of tissue nor the vagaries of tissue constituency. Too thick, then the fixation may be equated with an underfixed specimen. Too much connective tissue (eg fibrosis) may impede fixation giving an under-fixed specimen. Hope you all had a good weekend Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jasper, Thomas G. Sent: Saturday, 16 June 2007 6:32 AM To: Lott, Robert Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Fixation for Her-2/neu -Where's the Beef??? Hey Robert, I've been informed from our pathologists that over-fixation of breast tissue (more than 48 hours) will potentially mask Her2 staining. This in turn impacts our FISH staining, as cases for FISH are selected based on Her2 results. The fear then, is that cases which should've reflexed to FISH will not. The over-fixation could result in under-expression of Her2 run cases. Sounds a bit contradictory but that's how I understand it. I do have to agree with you, my concern would be under-fixation vs. over-fixation. But, I guess under-fixation is more readily apparent and easier to address than over-fixation. This will be a new thing for everybody and we're looking at a new mandatory week-end shift because of it (oh joy). Anyway, this is what I've been told and how I understand it, good luck down there in Bama. Tom Jasper HT (ASCP) BAS SMDC Duluth, MN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Lott, Robert Sent: Friday, June 15, 2007 11:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fixation for Her-2/neu -Where's the Beef??? Hello Everyone, .....been a lot of talk about this already, I'm aware of that. Now I want to know if there is any "real" scientific" basis for the new CAP guideline for Her-2 IHC fixation that states "No less than 6 hrs. and no more than 48 hrs. in aqueous NFB." Any documented, peer-reviewed, published data??? My sense is that this guideline really does represent best practice. Most of us do well to get our breast specimens fixed for 6 hrs. in NBF!! However, is there really some difference in performance of the Her-2 IHC assay if a breast case is fixed for 48 hrs vs. one fixed for 54 hrs. or 72 hrs. (i.e. weekend/ holiday scenario). UNDER-fixation would seem to be so much more of issue than OVER-fixation. It's this top end that bothers me so much!!! I am not aware of real data to support this new guideline! Does anyone out there know anything different????? Robert Robert L. Lott, HTL(ASCP) Manager, Anatomic Pathology LabFirst / Trinity Medical Center - formerly Montclair Baptist Medical Center 800 Montclair Road Birmingham, AL 35213 205-592-5388 phone 205-592-5646 fax robert.lott@triadhospitals.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From Marilyn.Tyler <@t> uct.ac.za Mon Jun 18 01:07:14 2007 From: Marilyn.Tyler <@t> uct.ac.za (Marilyn Tyler) Date: Mon Jun 18 01:07:38 2007 Subject: [Histonet] Negative control IHC Message-ID: <46763D31.A78E.0090.0@uct.ac.za> Morning Histonetters What do you use for negative controls for FFPE tissue in IHC, apart from the reagent negative. Thanks Marilyn From louise.renton <@t> gmail.com Mon Jun 18 02:03:08 2007 From: louise.renton <@t> gmail.com (louise renton) Date: Mon Jun 18 02:03:13 2007 Subject: [Histonet] thanks IHC background Message-ID: Thanks to all who reponded to my query regarding excessive background. I still believe that most of the problem is to do with the decalcification of the tissue, as I have got beaytiful clean & specific staining in controlled tissues with the sam Abs and Tris/Tween wash buffer. Well, we do our best with what we got...and try to get the researchers to understand that you gotta plan ahead! -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From ree3 <@t> leicester.ac.uk Mon Jun 18 03:31:21 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Mon Jun 18 03:31:30 2007 Subject: [Histonet] Hard Antibodies! In-Reply-To: <8C97D971CE35C2A-554-49E1@webmail-dd03.sysops.aol.com> References: <8C97D971CE35C2A-554-49E1@webmail-dd03.sysops.aol.com> Message-ID: For people like myself who work primarily on animal tissues every antibody can be a "hard antibody"!!. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of bjdewe@aol.com Sent: 15 June 2007 20:04 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Hard Antibodies! Does anyone have any antibodies they consider especially hard to do? I'm curious because I know what we have trouble with in our lab but am curious if anyone else has the same issues... Thanks in advance for your input! Cheers, Lorie Live as if you were to die tomorrow. Learn as if you were to live forever. - Gandhi - ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Jun 18 07:27:32 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jun 18 07:27:40 2007 Subject: [Histonet] Negative control IHC In-Reply-To: <46763D31.A78E.0090.0@uct.ac.za> Message-ID: <308695.43999.qm@web61220.mail.yahoo.com> A section from the same tested case Ren? J. Marilyn Tyler wrote: Morning Histonetters What do you use for negative controls for FFPE tissue in IHC, apart from the reagent negative. Thanks Marilyn _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Park yourself in front of a world of choices in alternative vehicles. Visit the Yahoo! Auto Green Center. From DOOLEEO <@t> shands.ufl.edu Mon Jun 18 09:26:26 2007 From: DOOLEEO <@t> shands.ufl.edu (Elaine Dooley) Date: Mon Jun 18 09:26:51 2007 Subject: [Histonet] Procollagen I Antibody Message-ID: <46765DD202000097000066AD@gw-fs1.shands.ufl.edu> Dear Histonetters and Vendors, Does anyone out there know of a Vendor for anti-Procollagen 1. I would like one that works on formalin fixed parraffin embedded tissue if possible. One of our pathologists wants it because he says it is better than factor 13a in differentiating DSFP from DF. Thanks in advance Elaine Dooley Shands Teaching Hospital Gainesville FL 352-265-0111 ext 72117 From judi.ford <@t> roche.com Mon Jun 18 09:50:58 2007 From: judi.ford <@t> roche.com (Ford, Judi) Date: Mon Jun 18 09:51:15 2007 Subject: [Histonet] unsubscribe Message-ID: Please take me off the list. I'm on vacation. Thanks, Judi Ford From gcallis <@t> montana.edu Mon Jun 18 10:04:54 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Jun 18 10:04:19 2007 Subject: [Histonet] Negative control IHC In-Reply-To: <46763D31.A78E.0090.0@uct.ac.za> References: <46763D31.A78E.0090.0@uct.ac.za> Message-ID: <6.0.0.22.1.20070618084545.01b44008@gemini.msu.montana.edu> Marilyn, Reagent negative or buffer is NOT a true negative control. You need to have the immunoglobulin, at the same working concentration as your primary antibody, which matches a primary antibody host immunogloblin (either whole or an immunoglobulin isotype). The only time we use PBS, then called a NULL control, is when we test our staining system for background sources. IF you use PBS as a negative control in a submitted manuscript, a reviewer could very well say a proper negative was not used and you would have to repeat the work. This happened to us when using normal serum, when the reviewer demanded an immunoglobulin negative control, in particular, an immunoglobulin isotype. This resulted in our no longer using normal serums anymore. Some people in the clinical area use an irrelevant antibody that will not stain anything in the tissue but provides the immunoglobulin needed. However, with murine or rat species, it is very difficult to find an irrelevant rat or hamster antimouse that doesn't light up some mouse/rat tissue component. Consequently, we use an immunoglobulin control from Rat or hamster (paying attention to WHICH hamster species here) immunoglobulin, IgG, IgG isotype, IgM, Armenian Hamster Ig, Goldern Syrian IgG. Many of these are conjugated to biotin and/or fluorophores for special applications when working with a biotinylated primary or a fluorophore that is directly conjugated to the primary. You can purchase negative immunglobulin controls readily from any vendor selling antibodies. You will find more answers to your question in Histonet archives, as this has been asked many times. At 12:07 AM 6/18/2007, you wrote: >Morning Histonetters >What do you use for negative controls for FFPE tissue in IHC, apart from >the reagent negative. >Thanks >Marilyn Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From pruegg <@t> ihctech.net Mon Jun 18 10:10:30 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Jun 18 10:10:29 2007 Subject: [Histonet] anti rat cd34 Message-ID: <004501c7b1ba$d03adb20$6501a8c0@Patsy> This came up a while ago but I must have missed any answers. Does anyone know of an anti rat cd34 that works on ffpe rat tissue? Thanks, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC 12635 Montview Blvd. Ste.215 Aurora, Colorado 80045 Phone: 720-859-4060 Fax: 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org From njoydobro <@t> aol.com Mon Jun 18 10:54:41 2007 From: njoydobro <@t> aol.com (njoydobro@aol.com) Date: Mon Jun 18 10:54:53 2007 Subject: [Histonet] Cam 5.2 Message-ID: <8C97FD81E278F13-1770-B309@webmail-de10.sysops.aol.com> Does anyone have a protocol for BD's Cam 5.2 on the Ventana Benchmark XT? THanks, Gene ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From Tiffany.Price <@t> thomaswv.org Mon Jun 18 10:31:56 2007 From: Tiffany.Price <@t> thomaswv.org (Price, Tiffany) Date: Mon Jun 18 11:01:20 2007 Subject: [Histonet] release of Pathology specimens Message-ID: <7B5F5D9A00739F43A1819403EC7CF49103C322FE@thm-mail.thomaswv.org> Does anyone have a policy/procedure for the release of pathology specimens to patients? (placenta, extremities, etc) Thanks Tiffany Confidentiality Notice: This email message including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privilege information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy ALL copies of the message. From SDrew <@t> uwhealth.org Mon Jun 18 11:26:44 2007 From: SDrew <@t> uwhealth.org (Drew Sally A.) Date: Mon Jun 18 11:29:56 2007 Subject: [Histonet] Cam 5.2 In-Reply-To: <8C97FD81E278F13-1770-B309@webmail-de10.sysops.aol.com> Message-ID: We use Protease 1 for 8" and 32" ttn at 37 degrees Sally Ann Drew, MT(ASCP) IHC/ISH Laboratory University of Wisconsin Hosp. & Clinics Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of njoydobro@aol.com Sent: Monday, June 18, 2007 10:55 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Cam 5.2 Does anyone have a protocol for BD's Cam 5.2 on the Ventana Benchmark XT? THanks, Gene ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Mon Jun 18 11:46:59 2007 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Mon Jun 18 11:47:03 2007 Subject: [Histonet] Cam 5.2 In-Reply-To: <8C97FD81E278F13-1770-B309@webmail-de10.sysops.aol.com> Message-ID: Here you go Gene. 1 drop of antibody for 32" at 42degrees C with Protease 1/8" and AB block and HemII/8". Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of njoydobro@aol.com Sent: Monday, June 18, 2007 10:55 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Cam 5.2 Does anyone have a protocol for BD's Cam 5.2 on the Ventana Benchmark XT? THanks, Gene ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jjohnson <@t> crispregional.org Mon Jun 18 11:56:04 2007 From: jjohnson <@t> crispregional.org (Jennifer Johnson) Date: Mon Jun 18 11:56:13 2007 Subject: [Histonet] I need a vacation! Message-ID: <000001c7b1c9$8f4ed6c0$2082010a@main.crispregional.org> Is there anybody in the South Georgia area that would like to work one week for me while I go on vacation? From dcrippen <@t> buckinstitute.org Mon Jun 18 11:58:56 2007 From: dcrippen <@t> buckinstitute.org (Danielle Crippen) Date: Mon Jun 18 11:59:04 2007 Subject: [Histonet] unsubscribe Message-ID: From timothy.m.coskran <@t> pfizer.com Mon Jun 18 13:54:54 2007 From: timothy.m.coskran <@t> pfizer.com (Coskran, Timothy M) Date: Mon Jun 18 13:55:05 2007 Subject: [Histonet] Negative control IHC Message-ID: <3F4063B77CC1F445933078B93A0A0C720257B03A@groamrexm05.amer.pfizer.com> What do you do when you have a polyclonal antibody for which the vendor has not determined an Ig concentration? It's easy to match concentration and isotype for a monoclonal but can be impossible for a polyclonal. If we have an Ig concentration, we will match with Rabbit IgG. If not, do you count on a buffer control, irrelevant antibody (not concentration matched), or normal sera? Lots of choices, not sure what is the best for this situation. Tim Coskran Pfizer ---------------------------------------------------------------------- LEGAL NOTICE Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this E-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately. From aselmeczi <@t> eyes.arizona.edu Mon Jun 18 13:55:49 2007 From: aselmeczi <@t> eyes.arizona.edu (Aniko Selmeczi) Date: Mon Jun 18 13:56:50 2007 Subject: [Histonet] (no subject) Message-ID: <4620F5127F9FDE4B80B4A31170CE9BA11A4026@ad2.eyes.arizona.edu> Please take me temporarily off from the list. Thank you, Aniko Selmeczi Aniko Selmeczi Research Specialist University of Arizona, Ophthalmology and Vision Science aselmeczi@eyes.arizona.edu From innvx <@t> sbcglobal.net Mon Jun 18 14:12:41 2007 From: innvx <@t> sbcglobal.net (INNOVEX BIOSCIENCES) Date: Mon Jun 18 14:12:51 2007 Subject: [Histonet] RE: Password Message-ID: <755955.50218.qm@web82004.mail.mud.yahoo.com> I for got password... Innovex , phonne #: 1-800-6227808 Zara Innovex Biosciences From innvx <@t> sbcglobal.net Mon Jun 18 14:15:18 2007 From: innvx <@t> sbcglobal.net (INNOVEX BIOSCIENCES) Date: Mon Jun 18 14:18:57 2007 Subject: [Histonet] Animal Tissue Stat- IHC Workshop Message-ID: <803537.76339.qm@web82003.mail.mud.yahoo.com> I N N O V E X B I O S C I E N C E S WORKSHOP ANNOUNCEMENT Animal Tissue STAT-IHC staining workshop From primary to microscope under 2 hours PRESENTED by Innovex Biosciences San Francisco, Saturday August 18, 2007, 1:00-4:30 pm LEVEL: Open to all levels This is a comprehensive hands-on wet workshop designated to address the major issues facing the veterinary Histologists that perform animal tissue IHC staining. The topics covered will include eradication of background staining in animal tissues, the techniques for lowering the primary antibody incubation time and lowering the entire staining run for fast turn-around time. Other topics covered will include the selection of appropriate secondary antibodies and co-reagents for IHC staining of animal tissues. This workshop will further address the STAT staining technologies available for background ?free quick IHC staining of animal tissues in less than 2 hours. Other covered topics will include novel amplification technologies for maximizing optimal staining results and eliminating troubleshooting. The application of STAT-IHC reagents and automated stainers will also be explored. This workshop is CEU approved CEU: This workshop is CEU approved for 3 contact hours FEE: This workshop is offered free of charge BENEFICIAL TO: Veterinarian pathologists and histologists, Veterinary IHC Practitioners, Veterinary researchers, Research histotechnologists and research immunohistochemists and to all Cost and time conscious Labs WORKSHOP INSTRUCTORS: Zahra Naser, Ph.D.; M.S, with over 20 years of experience in Immunology, IHC, flow cytometry and Biochemistry and chemistry. Loralei Dewe: Co-Director of Confocal Microscope Core at Shriners Hospital, 10+ years experience with Histology, Immunochemistry and Light and Fluorescent Imaging. SPACE: Space is limited to 22 people for maximum personal attention. Reserve your space as soon as possible. LOCATION: San Francisco, University of California, San Francisco (UCSF), Laurel heights campus, Parking: Parking available for a fee. DATE: Saturday August 18, 2007, 1:00-4:30 pm TO REGISTER On line, Visit innvx.com, click on Register for workshop on home page. TO REGISTER via Phone: Call us at 1-800-622-7808, Main 510-234-6600. TO REGISTER via fax: Fill out the enclosed registration form and fax it to 510-234-4591. HOTEL INFORMATION: If you require accommodation, please contact Innovex immediately. Also upon registration, hotel and transportation will be emailed or Faxed to you. Contact Innovex at 1-800-622-7808 OR 510-234-6600 Web: innx.com Disclaimer: Innovex reserves all rights for admission of the participants. From BoozerKA <@t> ah.org Mon Jun 18 14:20:35 2007 From: BoozerKA <@t> ah.org (Kathleen Boozer) Date: Mon Jun 18 14:21:16 2007 Subject: [Histonet] release of Pathology specimens In-Reply-To: <7B5F5D9A00739F43A1819403EC7CF49103C322FE@thm-mail.thomaswv.org> References: <7B5F5D9A00739F43A1819403EC7CF49103C322FE@thm-mail.thomaswv.org> Message-ID: <46767892.4AA8.00C0.0@ah.org> Tissue is released for cultural or religious reasons only. When released, a 5 minute talk about the hazards of formalin is addressed and signed off by the tech and patient. >>> "Price, Tiffany" 06/18/2007 08:31 >>> Does anyone have a policy/procedure for the release of pathology specimens to patients? (placenta, extremities, etc) Thanks Tiffany Confidentiality Notice: This email message including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privilege information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy ALL copies of the message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjr6 <@t> psu.edu Mon Jun 18 14:23:28 2007 From: rjr6 <@t> psu.edu (Roberta Horner) Date: Mon Jun 18 14:23:33 2007 Subject: [Histonet] teeth Message-ID: I just received some teeth to section can anyone tell me if I can just use any decalcification solution or is there something special to do with teeth? These are from a calf and it has been over 20 years since I took class for histology and in the back of my mind I seem to remember that you need to do something different. Roberta Horner HT, HTL Penn State University Animal Diagnostic Lab From beta.amyloid <@t> gmail.com Mon Jun 18 14:25:28 2007 From: beta.amyloid <@t> gmail.com (Katherine Cano) Date: Mon Jun 18 14:25:35 2007 Subject: [Histonet] Problems with rhodanine Message-ID: <7f9d20de0706181225l1d69445eq34e8924614153f07@mail.gmail.com> Hi all, I'm a graduate student in a neuropsychology lab, and we are having some problems with our histology. I was hoping someone might have some advice. My lab is doing staining for metals in fresh frozen mouse brain, sectioned at 10u or 20u. Some of our animals are fed high copper supplements and we see high copper in areas of our tissue with other methods, so we are trying to pilot rhodanine for copper. However, the protocols we have for rhodanine give conflicting instructions. We have unfixed, fresh frozen tissue, which is part of the problem, since ALL our protocols are for FFPE tissue. Two of the protocols call for microwave method, but we don't have an appropriate microwave so we are using the conventional oven method, which says to heat the slides for 18 hours at 37 or 30 degrees centigrade. When we looked at the coverslipped slides, it looked as though the tissue had washed off in place. I have seen this happen before with immuno, and my histology professor said it was a problem with the slide subbing; however, we are using Erie Superfrost Plus slides, which I did not think would have that problem. We DID end up moving the slides between buildings, so maybe the tissue was jostled too much during transport and that's why it washed off? Does anyone have any experience with rhodanine on fresh frozen tissue? Do you need a special protocol? Does it just not work? Any input would be appreciated. Thanks in advance, Katherine From ecpenaflor <@t> ucsf.neuroimmunol.org Mon Jun 18 14:51:10 2007 From: ecpenaflor <@t> ucsf.neuroimmunol.org (Vin Penaflor) Date: Mon Jun 18 14:51:57 2007 Subject: [Histonet] Question: MHC Class II Immunofluorscent Staining on CNS tissue. Message-ID: Hi, I am currently having an issue with trying to stain MHC Class II (IaB/AF6 Clone) on mouse brain tissue for confocal microscopy immunofluorescence. I have been trying to use BD Pharmingens, Biotinylated Anti mouse I-Ab coupled with a secondary molecular probes streptavidin-594. So far I have not been able to get the Iab staining to work. I get completely no staining. I am curious if anyone has a successful protocol with conditions that would produce a very bright staining on confocal microscopy with minimal background for this antibody. Any help would be greatly appreciated. I have tried different perfusion techniques, freezing techniques, fixations, wash steps, and dilutions of the antibody and so far no luck. If anyone has conditions or a protocol that works well with this particular antibody on brain or spinal cord tissue, I would greatly appreciate any input. I have tried using the primary and secondary antibody on mouse spleen tissue and it works PERFECTLY, it's just that somehow in the brain I cannot get it to work. Also if there are better antibodies/secondary from other companies I should try that would also be helpful! Thanks in advance! From FUNKM <@t> mercyhealth.com Mon Jun 18 15:12:34 2007 From: FUNKM <@t> mercyhealth.com (Marcia Funk) Date: Mon Jun 18 15:12:58 2007 Subject: [Histonet] Release Message-ID: <4676A0E2020000AC000036F5@nodcmsngwia1.trinity-health.org> If we need to release tissue to a patient usually bone from knee, it is placed in 95% alc and then the container cap is coated with paraffin. The patient signs a consent form starting the tissue has been released to them. We have had a extremity asked but it was released to a funeral home. Your institution should have a policy that should include what or if they can be released some States I think have laws that you must follow. Hope this might help. Mrf From rjbuesa <@t> yahoo.com Mon Jun 18 15:28:18 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jun 18 15:28:22 2007 Subject: [Histonet] teeth In-Reply-To: Message-ID: <212013.12961.qm@web61220.mail.yahoo.com> The problem with teeth is the enamel. The best fixing/"softening" solution is that of Bodecker (1937): methanol ---100 mL celloidin --6 mL nitric acid---4.5 mL Ren? J. Roberta Horner wrote: I just received some teeth to section can anyone tell me if I can just use any decalcification solution or is there something special to do with teeth? These are from a calf and it has been over 20 years since I took class for histology and in the back of my mind I seem to remember that you need to do something different. Roberta Horner HT, HTL Penn State University Animal Diagnostic Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Moody friends. Drama queens. Your life? Nope! - their life, your story. Play Sims Stories at Yahoo! Games. From rjbuesa <@t> yahoo.com Mon Jun 18 15:32:11 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jun 18 15:32:15 2007 Subject: [Histonet] Problems with rhodanine In-Reply-To: <7f9d20de0706181225l1d69445eq34e8924614153f07@mail.gmail.com> Message-ID: <184663.13599.qm@web61220.mail.yahoo.com> Katherin: The best copper method is Timm's (with ammonium sulfide) and silver nitrate. Rhodamine is usually "very tricky". Ren? J. Katherine Cano wrote: Hi all, I'm a graduate student in a neuropsychology lab, and we are having some problems with our histology. I was hoping someone might have some advice. My lab is doing staining for metals in fresh frozen mouse brain, sectioned at 10u or 20u. Some of our animals are fed high copper supplements and we see high copper in areas of our tissue with other methods, so we are trying to pilot rhodanine for copper. However, the protocols we have for rhodanine give conflicting instructions. We have unfixed, fresh frozen tissue, which is part of the problem, since ALL our protocols are for FFPE tissue. Two of the protocols call for microwave method, but we don't have an appropriate microwave so we are using the conventional oven method, which says to heat the slides for 18 hours at 37 or 30 degrees centigrade. When we looked at the coverslipped slides, it looked as though the tissue had washed off in place. I have seen this happen before with immuno, and my histology professor said it was a problem with the slide subbing; however, we are using Erie Superfrost Plus slides, which I did not think would have that problem. We DID end up moving the slides between buildings, so maybe the tissue was jostled too much during transport and that's why it washed off? Does anyone have any experience with rhodanine on fresh frozen tissue? Do you need a special protocol? Does it just not work? Any input would be appreciated. Thanks in advance, Katherine _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! oneSearch: Finally, mobile search that gives answers, not web links. From Barry.R.Rittman <@t> uth.tmc.edu Mon Jun 18 15:41:04 2007 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Mon Jun 18 15:41:07 2007 Subject: [Histonet] teeth In-Reply-To: Message-ID: Roberta Would you let us know what information you need to obtain from the teeth as there are numerous techniques. If you would like to discuss this in detail you are welcome to contact me at 713-500-4134 office or 713 -542 -8108 cellular. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roberta Horner Sent: Monday, June 18, 2007 2:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] teeth I just received some teeth to section can anyone tell me if I can just use any decalcification solution or is there something special to do with teeth? These are from a calf and it has been over 20 years since I took class for histology and in the back of my mind I seem to remember that you need to do something different. Roberta Horner HT, HTL Penn State University Animal Diagnostic Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Mon Jun 18 16:13:37 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Jun 18 16:13:03 2007 Subject: unknown concetration antibody Re: [Histonet] Negative control IHC In-Reply-To: <3F4063B77CC1F445933078B93A0A0C720257B03A@groamrexm05.amer. pfizer.com> References: <3F4063B77CC1F445933078B93A0A0C720257B03A@groamrexm05.amer.pfizer.com> Message-ID: <6.0.0.22.1.20070618143723.01b3eb60@gemini.msu.montana.edu> Tim, If the vendor can't provide a concentration (shame on them!), we try to use the IgG matching the host of the antibody. Ascites is another story. This is always a guessing game, but PBS buffer is still never used as a negative control. Maybe with a polyclonal, normal serum could be used or an irrelevant antibody - a dilemma to be sure and a choice one has to make. We had one antibody where we decided to use normal serum at same concentration as polyclonal even though that is not a choice we like very much. The vendor (some obscure company) said the antibody was just antisera, and nothing about purification. Frustration ran high, our guess will be as good as the next person's. Here is an email I kept from Deena Hepburn on how to determine protein concentration on ascites and antibodies, and may be important with a pending publication. ************************************************************************************************************************************ Here is the information for the protein assay I promised. I saw this one and it looked like the easiest to do. There are no reagents to prepare. I usually use the BCA or Comassie kit assay but they are a bit more complicated. You will also need to purchase the standards. For antibodies, I would buy the pre-diluted Protein Assay standard : Bovine Gamma Globulin Set. With this assay you will need to dilute your ascites quite a bit. Assuming your ascites is about 10mg/ml, I would do a dilution series something like: 1:10, 1:100, 1:250, 1:500, 1:1000, 1:2500, 1:5000 (or something with a wide range - you should do a range to make sure you are on the curve). You should read them on a plate reader but for a rough guess you can compare the dilution with the standard curve to match colors. For example: If color of your 1:100 dilution matches the color of the 125ug/ml standard your concentration would be approximately 12.5mg/ml 125ug/ml (protein standard) X 100 (dilution factor) = 12,500ug/ml or 12.5mg/ml Company: Pierce Web address: www.piercenet.com http://www.piercenet.com/products/browse.cfm?fldID=02020110 (assay) http://www.piercenet.com/products/browse.cfm?fldID=02020108 (standards) Feel free to call me if you need any additional help. Deena Hepburn Asst. Senior Biologist Eli Lilly and Co. Indianapolis, In 46285 317-276-9374 *********************************************************************************************************************** Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 Some At 12:54 PM 6/18/2007, you wrote: >What do you do when you have a polyclonal antibody for which the vendor >has not determined an Ig concentration? It's easy to match >concentration and isotype for a monoclonal but can be impossible for a >polyclonal. If we have an Ig concentration, we will match with Rabbit >IgG. If not, do you count on a buffer control, irrelevant antibody (not >concentration matched), or normal sera? Lots of choices, not sure what >is the best for this situation. > > > >Tim Coskran > >Pfizer From gcallis <@t> montana.edu Mon Jun 18 16:21:18 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Jun 18 16:20:45 2007 Subject: [Histonet] Problems with rhodanine In-Reply-To: <7f9d20de0706181225l1d69445eq34e8924614153f07@mail.gmail.co m> References: <7f9d20de0706181225l1d69445eq34e8924614153f07@mail.gmail.com> Message-ID: <6.0.0.22.1.20070618151635.01b3e458@gemini.msu.montana.edu> Katherine, Are you even fixing the frozen sections AFTER you pick them up onto the slides and before staining? Sounds as though unfixed frozen sections are washing off the slides At 01:25 PM 6/18/2007, you wrote: >Hi all, > >I'm a graduate student in a neuropsychology lab, and we are having some >problems with our histology. I was hoping someone might have some advice. > >My lab is doing staining for metals in fresh frozen mouse brain, sectioned >at 10u or 20u. Some of our animals are fed high copper supplements and we >see high copper in areas of our tissue with other methods, so we are trying >to pilot rhodanine for copper. However, the protocols we have for rhodanine >give conflicting instructions. > >We have unfixed, fresh frozen tissue, which is part of the problem, since >ALL our protocols are for FFPE tissue. Two of the protocols call for >microwave method, but we don't have an appropriate microwave so we are using >the conventional oven method, which says to heat the slides for 18 hours at >37 or 30 degrees centigrade. > >When we looked at the coverslipped slides, it looked as though the tissue >had washed off in place. I have seen this happen before with immuno, and my >histology professor said it was a problem with the slide subbing; however, >we are using Erie Superfrost Plus slides, which I did not think would have >that problem. We DID end up moving the slides between buildings, so maybe >the tissue was jostled too much during transport and that's why it washed >off? > >Does anyone have any experience with rhodanine on fresh frozen tissue? Do >you need a special protocol? >Does it just not work? Any input would be appreciated. > >Thanks in advance, >Katherine >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From anthony <@t> histotechexchange.com Mon Jun 18 16:24:50 2007 From: anthony <@t> histotechexchange.com (anthony@histotechexchange.com) Date: Mon Jun 18 16:25:21 2007 Subject: [Histonet] HTs. Grossing Larger spcimens, Kidneys etc and the CAP regulations Message-ID: <3686.71.51.6.248.1182201913.squirrel@host4.wfdns.com> Dear All: I am trying to find out the requirements by CAP for a HT to gross larger specimens, such as whole kidneys and livers etc. This person has years of experience but has not sat the ASCP PA exam. The state requires no special training other than the OK from a Pathologist and to be under his/her supervision. I have looked at the cap guidelines amendments for 2006 and they state: Are all macroscopic tissue examinations performed by a pathologist or pathology resident, or under the supervision of a qualified pathologist? I am leaning toward them being able to gross lots of stuff, if the pathologist is satisfied with there competency) except for the specimens requiring staging of cancer severity. Even this if they have the experience and the pathologist is satisfied that the competency and experience is there. Am I wrong? NOTE: Two levels of complexity of macroscopic tissue examination are defined, as follows: ANP.11600 Phase II 1) Processing is defined as a tissue examination limited to description, inking and cutting of the specimen (if applicable), and submission of the entire specimen to histology. Tissue processing can be performed according to standardized protocols. Processing is generally limited to small specimens (skin ellipses, small biopsies, curettings, etc.) and does not require knowledge of anatomy. 2) Grossing (or gross examination) is defined as a tissue examination requiring a greater exercise of judgment and a knowledge of anatomy. Dissection of the specimen and selection of tissue samples for submission to histology are generally required. The specimen description is not necessarily standardized. Specific requirements for supervision of non-pathologists who process specimens, or assist in grossing specimens, are given below. COMMENTARY: N/A What is the difference between the requirements for the second class of specimen? ANP.11605 Phase II When individuals other than a pathologist or pathology resident process specimens, or assist in gross examinations, is the extent of their activities (including the types of specimens examined) defined in a documented protocol? NOTE: This protocol must list the specific types of specimens that non-pathologists are permitted to process, and for which non-pathologists are permitted to assist in the gross examination. The laboratory director is responsible for this protocol. ANP.12350 Phase II For specimens from definitive cancer resections, and in specific biopsies as outlined in the CAP Cancer Protocols, are all scientifically validated data elements needed for standard systems of grading, staging and prognostication included in the pathology report? NOTE: The pathology report must provide data that, within the confines of information available to the pathologist, is sufficient to allow appropriate grading and staging of neoplasms according to standard classification schemes. Lists of the scientifically validated data elements may be found in the CAP Cancer Protocols and Checklists. The use of these checklists is encouraged, but not required, providing that the required data elements are present in the report. The use of synoptic diagnostic reports is recommended, to ensure that all information relevant to staging and grading is included, and to facilitate interpretation of pathology reports by clinicians. COMMENTARY: N/A REFERENCE: College of American Pathologists. Practicing Pathology: Cancer Protocols. http://www.cap.org/cancerprotocols/protocols/intro.html. Yours truly, Anthony Williams HT (ASCP) Histotech Exchange LLC 19 Whitmore Street Lexington, VA 24450 T 1 877 464 8911 F 1 540 301 0071 anthony@histotechexchange.com www.histotechexchange.com From gprogers48 <@t> comcast.net Mon Jun 18 16:43:27 2007 From: gprogers48 <@t> comcast.net (Gary P Rogers) Date: Mon Jun 18 16:43:33 2007 Subject: [Histonet] USED EQUIPMENT Message-ID: I am looking for used Sakura tissue processors, Sakura tape coverslippers, Leica cryostats and stainers. Anyone that has old equipment they are not using and would like to get it out of the laboratory, please respond to: Gary P. Rogers DAOT Enterprises, Inc. Phone: (800) 717-0983 code 00 Office: (610) 627-0508 Fax: (610) 627-0841 Cell: (484) 886-6870 gprogers48@comcast.net Thanks in advance. From Jeannette.Mitchell <@t> vtmednet.org Mon Jun 18 17:47:31 2007 From: Jeannette.Mitchell <@t> vtmednet.org (Mitchell, Jeannette M.) Date: Mon Jun 18 17:48:36 2007 Subject: [Histonet] USED EQUIPMENT References: Message-ID: <8C7B2CE85A8CBA49B3FE05DE478412ED029DC6CA@FAHC14.fahc.fletcherallen.org> We have 3 IHC Autostainers that we are willing to sell. Please email me for details. Jeannette Mitchell FAHC Burlington,Vt ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Gary P Rogers Sent: Mon 6/18/2007 5:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] USED EQUIPMENT I am looking for used Sakura tissue processors, Sakura tape coverslippers, Leica cryostats and stainers. Anyone that has old equipment they are not using and would like to get it out of the laboratory, please respond to: Gary P. Rogers DAOT Enterprises, Inc. Phone: (800) 717-0983 code 00 Office: (610) 627-0508 Fax: (610) 627-0841 Cell: (484) 886-6870 gprogers48@comcast.net Thanks in advance. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This message, and any attachments, may contain information that is confidential, privileged, and/or protected from disclosure under state and federal laws that deal with the privacy and security of medical information. If you received this message in error or through inappropriate means, please reply to this message to notify the Sender that the message was received by you in error, and then permanently delete this message from all storage media, without forwarding or retaining a copy. From innvx <@t> sbcglobal.net Mon Jun 18 17:37:17 2007 From: innvx <@t> sbcglobal.net (INNOVEX BIOSCIENCES) Date: Mon Jun 18 18:20:26 2007 Subject: [Histonet] Workshop, San Francisco , Animal STAT-IHC workshop, FRIDAY, AUG 17 Message-ID: <538623.65176.qm@web82002.mail.mud.yahoo.com> I N N O V E X B I O S C I E N C E S FRIDAY, August 17, 2007 1:00 - 4:30 PM CEU APPROVED WORKSHOP (CEU APPROVED) FRIDAY AUGUST 17, 2007 1:00-4:30 pm Animal Tissue STAT-IHC staining workshop From primary to microscope under 2 hours PRESENTED by Innovex San Francisco, FRIDAY August 17, 2007, 1:00-4:30 pm LEVEL: Open to all levels This is a comprehensive hands-on wet workshop designated to address the major issues facing the veterinary Histologists that perform animal tissue IHC staining. The topics covered will include eradication of background staining in animal tissues, the techniques for lowering the primary antibody incubation time and lowering the entire staining run for fast turn-around time. Other topics covered will include the selection of appropriate secondary antibodies and co-reagents for IHC staining of animal tissues. This workshop will further address the STAT staining technologies available for background ?free quick IHC staining of animal tissues in less than 2 hours. Other covered topics will include novel amplification technologies for maximizing optimal staining results and eliminating troubleshooting. The application of STAT-IHC reagents and automated stainers will also be explored. This workshop is CEU approved CEU: This workshop is CEU approved for 3 contact hours FEE: This workshop is offered free of charge BENEFICIAL TO: Veterinarian pathologists and histologists, Veterinary IHC Practitioners, Veterinary researchers, Research histotechnologists and research immunohistochemists and to all Cost and time conscious Labs WORKSHOP INSTRUCTORS: Zahra Naser, Ph.D.; M.S, with over 20 years of experience in Immunology, IHC, flow cytometry and Biochemistry and chemistry. Loralei Dewe: Co-Director of Confocal Microscope Core at Shriners Hospital, 10+ years experience with Histology, Immunochemistry and Light and Fluorescent Imaging. SPACE: Space is limited to 22 people for maximum personal attention. Reserve your space as soon as possible. LOCATION: San Francisco, University of California, San Francisco (UCSF), Laurel heights campus Parking: Parking available for a fee. DATE: Friday August 17, 2007, 1:00-4:30 pm TO REGISTER On line, Visit innvx.com, click on Register for workshop on home page. TO REGISTER via Phone: Call us at 1-800-622-7808, Main 510-234-6600. TO REGISTER via fax: Fill out the enclosed registration form and fax it to 510-234-4591. HOTEL INFORMATION: If you require accommodation, please contact Innovex immediately. Also upon registration, hotel and transportation will be emailed or Faxed to you. Contact Innovex at 1-800-622-7808 OR 510-234-6600 Web: innx.com Disclaimer: Innovex reserves all rights for admission of the participants. From lpwenk <@t> sbcglobal.net Mon Jun 18 18:29:53 2007 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Mon Jun 18 18:30:10 2007 Subject: [Histonet] release of Pathology specimens In-Reply-To: <7B5F5D9A00739F43A1819403EC7CF49103C322FE@thm-mail.thomaswv.org> Message-ID: <000001c7b200$93d23700$0202a8c0@HPPav2> First of all, check with your Safety department, and find out if they have a policy about releasing specimens that are fresh/biohazardous or fixed/chemical hazard. The Safety department might already have a policy, or if they don't, might be willing to help you write one. You might need to confer with Epidemiology, also. Second, you might need to check with your Legal department. They can check into the legal requirements of your state. Have Safety and Legal talk with each other. In our institution, we had to write up a page to be given to someone requesting their specimen. This page explained about the department's need to hang onto specimens for CAP for 2 weeks after final diagnosis (so the request could not be honored until after that time), the dangers of formaldehdye, and the dangers of fresh specimens. That the specimen should not be removed from the container, and that the specimen must be kept out the reach of animals and children. The person requesting their specimen must read this page. Then we have a check list for the lab people to follow, about washing the specimen, placing in appropriate containers, putting on appropriate labels (formaldehyde warning or biohazard), verifying ID of person picking up specimen (that it belongs to them or their child), and getting pathologist's to sign to release the specimen. Then we have the patient sign a release form in which they acknowledge receiving the page of hazards, that they have read it, that they assume responsibility for following the instructions, and that they cannot hold the hospital or lab legally responsible for any injuries to anyone. All of this had to be approved by Legal, Safety, Lab Compliance, our department (histology, safety officer, pathologist over histology). In other words, all the pages went through many changes before everyone was satisfied. When you think about it, histotechs have to have yearly training on the safety of chemicals and formaldehyde, as well has biohazards. Why should the public be allowed to take home a specimen unawares? Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Price, Tiffany Sent: Monday, June 18, 2007 11:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] release of Pathology specimens Does anyone have a policy/procedure for the release of pathology specimens to patients? (placenta, extremities, etc) Thanks Tiffany Confidentiality Notice: This email message including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privilege information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy ALL copies of the message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> aol.com Mon Jun 18 20:31:43 2007 From: rsrichmond <@t> aol.com (rsrichmond@aol.com) Date: Mon Jun 18 20:31:52 2007 Subject: [Histonet] Re: release of pathology specimens In-Reply-To: <200706181300.8f84676ba18335@rly-md03.mx.aol.com> References: <200706181300.8f84676ba18335@rly-md03.mx.aol.com> Message-ID: <8C98028BA0859F0-180-BBE6@FWM-R06.sysops.aol.com> Tiffany Price (where?) asks: >>Does anyone have a policy/procedure for the release of pathology specimens to patients? (placenta, extremities, etc)<< I've never seen a policy written, though there probably needs to be. Extremities: Requests for amputated legs for burial in the family cemetery are fairly common where I practice in rural southern Appalachia. Expect such requests from highly observant Jews also. Get the help of the family funeral director - this is a situation they're comfortable with. Mastectomy specimens: I've never encountered this situation, but apparently some women want to bury them. Once again, you need a funeral director. Souvenir gallstones: This used to be very common - in one hospital we had to wash and bottle all the gallstones before we left for the day, in case a surgeon demanded them - but this practice seems to have been banned by the infection control people. Souvenir tonsils went the same way. Placentas: you don't want to know. Some lay accoucheurs require their patients to EAT their placentas (rather, their babies' placentas) like animals do. Several years ago while doing a locum in rural southern Misery I had an indignant call from a lay midwife in a distant town. Seems she'd had a delivery with a retained placenta, gotten an OB-GYN to remove it, and sent it to us for examination. What she was indignant about was that the hospital had put the placenta in formalin, and in that condition autoplacentophagy was impossible. Reference: Ober, William B. [of blest memory] A modest proposal for preventing choriocarcinoma among innocent mothers. Obstetrics and Gynecology 1968;31:866-9. Hope you're not reading Histonet over lunch! Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From jmaass <@t> frii.com Mon Jun 18 20:58:05 2007 From: jmaass <@t> frii.com (Janet Maass) Date: Mon Jun 18 20:58:11 2007 Subject: [Histonet] teeth References: Message-ID: <002601c7b215$46dc5290$0200a8c0@Janet03b999f> Roberta I would recommend 10% trifluoroacetic acid for teeth. The procedure is printed in "The Journal of Histotechnology/ Vol. 25, No. 3, September 2002. Article titled "Processing the Complete Canine Tooth". Janet Maass ----- Original Message ----- From: "Roberta Horner" To: Sent: Monday, June 18, 2007 1:23 PM Subject: [Histonet] teeth I just received some teeth to section can anyone tell me if I can just use any decalcification solution or is there something special to do with teeth? These are from a calf and it has been over 20 years since I took class for histology and in the back of my mind I seem to remember that you need to do something different. Roberta Horner HT, HTL Penn State University Animal Diagnostic Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cbass <@t> bidmc.harvard.edu Mon Jun 18 21:12:36 2007 From: cbass <@t> bidmc.harvard.edu (Caroline Bass) Date: Mon Jun 18 21:12:44 2007 Subject: [Histonet] quick and dirty scale bar Message-ID: Hey Guys, I'm trying to finish up some figures for a paper. One collaborator has given me 10X images that appear to have been taken from a standard digital camera. Can anyone suggest a quick and easy way to add a scale bar? Unfortunately the journal requires it and I just don't have it. I have a scale bar for a 40X picture from a different microscope/experiment, any chance that I can use this? I'm guessing it is not as simple as my 40X scale bar being 1/4 of the 10X? Sorry, but I am clueless about this... any suggestions would be appreciated. Thanks, Caroline From Marilyn.Tyler <@t> uct.ac.za Tue Jun 19 00:56:51 2007 From: Marilyn.Tyler <@t> uct.ac.za (Marilyn Tyler) Date: Tue Jun 19 00:57:16 2007 Subject: [Histonet] IHC control Message-ID: <46778C42.A78E.0090.0@uct.ac.za> Thanks to all for their advice Marilyn From ian.montgomery <@t> bio.gla.ac.uk Tue Jun 19 04:27:42 2007 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Tue Jun 19 04:27:50 2007 Subject: FW: [Histonet] quick and dirty scale bar Message-ID: <000301c7b254$168b8db0$6424d182@IBLS.GLA.AC.UK> Caroline, Ask the collaborator if they will take another micrograph of a scale bar using the same settings. If this is impractical, is there a red blood corpuscle in the field? The built in scale bar that will give an approximate figure. I was going too rant and rave about x10 and x40 images and how they bear no relationship to reality but that can keep, the crumpy crabbit auld man is going for coffee. Ian. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Caroline Bass Sent: 19 June 2007 03:13 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] quick and dirty scale bar Hey Guys, I'm trying to finish up some figures for a paper. One collaborator has given me 10X images that appear to have been taken from a standard digital camera. Can anyone suggest a quick and easy way to add a scale bar? Unfortunately the journal requires it and I just don't have it. I have a scale bar for a 40X picture from a different microscope/experiment, any chance that I can use this? I'm guessing it is not as simple as my 40X scale bar being 1/4 of the 10X? Sorry, but I am clueless about this... any suggestions would be appreciated. Thanks, Caroline _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From psoni <@t> post.harvard.edu Tue Jun 19 04:39:30 2007 From: psoni <@t> post.harvard.edu (Pankaj Krish Soni) Date: Tue Jun 19 04:39:42 2007 Subject: [Histonet] Formaldehyde: tissue degradation Message-ID: <003101c7b255$bdf9af90$0d01a8c0@IBMT41> Dear All We are trying to identify some research that studies the degradation of tissue kept in formaldehyde over long periods of time (over 5 years). Ultimately we are trying to assess the number of years beyond which the quality of tissue fixed in formaldehyde would not generally permit valid scientific evaluation. Does anyone know of any relevant research? Any help would be very much appreciated. Thank you. Krish Soni From oshel1pe <@t> cmich.edu Tue Jun 19 08:01:30 2007 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Tue Jun 19 08:01:44 2007 Subject: [Histonet] quick and dirty scale bar In-Reply-To: References: Message-ID: Caroline, No, you can't use the 40X scale bar, and yes, it's not that simple. Even if the image with the 40X scale bar were taken on the same microscope with the same camera and the same settings, it wouldn't be in an exact 1:4 relation with the 10X objective. Further, you don't know what if any zoom your collaborator used. If the image was taken with a standard digital camera (e.g., a Nikon Coolpix) through an eyepiece adapter, there is often vignetting around the edges. This prompts people to use the zoom function to eliminate this. Which of course means the image isn't "10X". Which it may not have been anyway, depending on the lens in the eyepiece adapter. The only way to get images with known scale bars is to take a micrograph of a stage micrometer **without changing the camera**, especially the zoom, for each objective used, *at the time the micrographs are taken*. Otherwise it is nearly impossible to replicate the zoom used. Calculations/measurements based on field of view aren't trustworthy for the same reasons -- you don't know the true field of view. If no zoom was used, then it may be possible to take a photo of a stage micrometer with the same objective, etc., and be OK. But I wouldn't trust it. Given what you have, there are really only 2 courses: first, is there a structure in the micrograph you have that has a well know size? RBCs don't count, they change size by a micrometer or more depending on how they're treated (and yes, still look like nice, biconcave discs). If there is, you can calculate magnification and therefore a scale bar from it. But likely not. The other recourse is to have you collaborator take the image again, and this time also take an image of a stage micrometer. Anything else is just guessing. Phil >Hey Guys, > >I'm trying to finish up some figures for a paper. One collaborator >has given me 10X images that appear to have been taken from a >standard digital camera. Can anyone suggest a quick and easy way to >add a scale bar? Unfortunately the journal requires it and I just >don't have it. I have a scale bar for a 40X picture from a >different microscope/experiment, any chance that I can use this? >I'm guessing it is not as simple as my 40X scale bar being 1/4 of >the 10X? > >Sorry, but I am clueless about this... any suggestions would be appreciated. > >Thanks, > >Caroline > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 From Terry.Marshall <@t> rothgen.nhs.uk Tue Jun 19 08:28:45 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Tue Jun 19 08:28:58 2007 Subject: [Histonet] quick and dirty scale bar Message-ID: <5C0BED61F529364E86309CADEA63FEF25E6C16@TRFT-EX01.xRothGen.nhs.uk> This is a tad bizarre. I had this problem recently, spending hours getting pictures just right for a junior's paper, then finding after submission, that they wanted magnification (useless information) and didn't want the helpful arrows which would point the uninitiated in the right direction. There was nothing for it but to start again. Proper pathology journals gave up putting magnifications or scale bars years ago (EM pictures excepted). It's just the other non-pathology journals which want it, and it's totally meaningless, empty information. I suggest you bullshit. Take a few red cells, call them 6mu, and put on a scale from that. Who's to know the difference? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Philip Oshel Sent: 19 June 2007 14:02 To: Histonet@Pathology.swmed.edu Subject: Re: [Histonet] quick and dirty scale bar Caroline, No, you can't use the 40X scale bar, and yes, it's not that simple. Even if the image with the 40X scale bar were taken on the same microscope with the same camera and the same settings, it wouldn't be in an exact 1:4 relation with the 10X objective. Further, you don't know what if any zoom your collaborator used. If the image was taken with a standard digital camera (e.g., a Nikon Coolpix) through an eyepiece adapter, there is often vignetting around the edges. This prompts people to use the zoom function to eliminate this. Which of course means the image isn't "10X". Which it may not have been anyway, depending on the lens in the eyepiece adapter. The only way to get images with known scale bars is to take a micrograph of a stage micrometer **without changing the camera**, especially the zoom, for each objective used, *at the time the micrographs are taken*. Otherwise it is nearly impossible to replicate the zoom used. Calculations/measurements based on field of view aren't trustworthy for the same reasons -- you don't know the true field of view. If no zoom was used, then it may be possible to take a photo of a stage micrometer with the same objective, etc., and be OK. But I wouldn't trust it. Given what you have, there are really only 2 courses: first, is there a structure in the micrograph you have that has a well know size? RBCs don't count, they change size by a micrometer or more depending on how they're treated (and yes, still look like nice, biconcave discs). If there is, you can calculate magnification and therefore a scale bar from it. But likely not. The other recourse is to have you collaborator take the image again, and this time also take an image of a stage micrometer. Anything else is just guessing. Phil >Hey Guys, > >I'm trying to finish up some figures for a paper. One collaborator has >given me 10X images that appear to have been taken from a standard >digital camera. Can anyone suggest a quick and easy way to add a scale >bar? Unfortunately the journal requires it and I just don't have it. >I have a scale bar for a 40X picture from a different >microscope/experiment, any chance that I can use this? >I'm guessing it is not as simple as my 40X scale bar being 1/4 of the >10X? > >Sorry, but I am clueless about this... any suggestions would be appreciated. > >Thanks, > >Caroline > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nair.ashwin <@t> gmail.com Tue Jun 19 08:32:40 2007 From: nair.ashwin <@t> gmail.com (Ashwin Nair) Date: Tue Jun 19 08:32:45 2007 Subject: [Histonet] quick and dirty scale bar In-Reply-To: References: Message-ID: Hi, You could try clicking the gridlines on a hemacytometer at 10X magnification. You could then use Jruler (which I believe you can download online) and determine the number of pixels that make up a certain length at 10X mag. Draw a line measuring the same number of pixels using photoshop on to the images that you received from the collaborator. I hope this helps. Regards Ashwin On 6/19/07, Philip Oshel wrote: > > Caroline, > > No, you can't use the 40X scale bar, and yes, it's not that simple. > Even if the image with the 40X scale bar were taken on the same > microscope with the same camera and the same settings, it wouldn't be > in an exact 1:4 relation with the 10X objective. > Further, you don't know what if any zoom your collaborator used. If > the image was taken with a standard digital camera (e.g., a Nikon > Coolpix) through an eyepiece adapter, there is often vignetting > around the edges. This prompts people to use the zoom function to > eliminate this. Which of course means the image isn't "10X". > Which it may not have been anyway, depending on the lens in the > eyepiece adapter. > The only way to get images with known scale bars is to take a > micrograph of a stage micrometer **without changing the camera**, > especially the zoom, for each objective used, *at the time the > micrographs are taken*. Otherwise it is nearly impossible to > replicate the zoom used. Calculations/measurements based on field of > view aren't trustworthy for the same reasons -- you don't know the > true field of view. > If no zoom was used, then it may be possible to take a photo of a > stage micrometer with the same objective, etc., and be OK. But I > wouldn't trust it. > > Given what you have, there are really only 2 courses: first, is there > a structure in the micrograph you have that has a well know size? > RBCs don't count, they change size by a micrometer or more depending > on how they're treated (and yes, still look like nice, biconcave > discs). If there is, you can calculate magnification and therefore a > scale bar from it. > But likely not. The other recourse is to have you collaborator take > the image again, and this time also take an image of a stage > micrometer. > Anything else is just guessing. > > Phil > > >Hey Guys, > > > >I'm trying to finish up some figures for a paper. One collaborator > >has given me 10X images that appear to have been taken from a > >standard digital camera. Can anyone suggest a quick and easy way to > >add a scale bar? Unfortunately the journal requires it and I just > >don't have it. I have a scale bar for a 40X picture from a > >different microscope/experiment, any chance that I can use this? > >I'm guessing it is not as simple as my 40X scale bar being 1/4 of > >the 10X? > > > >Sorry, but I am clueless about this... any suggestions would be > appreciated. > > > >Thanks, > > > >Caroline > > > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- > Philip Oshel > Microscopy Facility Supervisor > Biology Department > 024C Brooks Hall > Central Michigan University > Mt. Pleasant, MI 48859 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Thank you. Ashwin Nair From rjbuesa <@t> yahoo.com Tue Jun 19 08:33:56 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jun 19 08:34:05 2007 Subject: [Histonet] quick and dirty scale bar In-Reply-To: <5C0BED61F529364E86309CADEA63FEF25E6C16@TRFT-EX01.xRothGen.nhs.uk> Message-ID: <418859.47176.qm@web61213.mail.yahoo.com> I once had that problem also and this is how I solved it: on a glossy print of the photo I DRAW (with India ink) the appropriate scale with the correct magnification and prepared a "JPEG" file with my scanner which could be used by the publisher. Ren? J. "Marshall Terry Dr, Consultant Histopathologist" wrote: This is a tad bizarre. I had this problem recently, spending hours getting pictures just right for a junior's paper, then finding after submission, that they wanted magnification (useless information) and didn't want the helpful arrows which would point the uninitiated in the right direction. There was nothing for it but to start again. Proper pathology journals gave up putting magnifications or scale bars years ago (EM pictures excepted). It's just the other non-pathology journals which want it, and it's totally meaningless, empty information. I suggest you bullshit. Take a few red cells, call them 6mu, and put on a scale from that. Who's to know the difference? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Philip Oshel Sent: 19 June 2007 14:02 To: Histonet@Pathology.swmed.edu Subject: Re: [Histonet] quick and dirty scale bar Caroline, No, you can't use the 40X scale bar, and yes, it's not that simple. Even if the image with the 40X scale bar were taken on the same microscope with the same camera and the same settings, it wouldn't be in an exact 1:4 relation with the 10X objective. Further, you don't know what if any zoom your collaborator used. If the image was taken with a standard digital camera (e.g., a Nikon Coolpix) through an eyepiece adapter, there is often vignetting around the edges. This prompts people to use the zoom function to eliminate this. Which of course means the image isn't "10X". Which it may not have been anyway, depending on the lens in the eyepiece adapter. The only way to get images with known scale bars is to take a micrograph of a stage micrometer **without changing the camera**, especially the zoom, for each objective used, *at the time the micrographs are taken*. Otherwise it is nearly impossible to replicate the zoom used. Calculations/measurements based on field of view aren't trustworthy for the same reasons -- you don't know the true field of view. If no zoom was used, then it may be possible to take a photo of a stage micrometer with the same objective, etc., and be OK. But I wouldn't trust it. Given what you have, there are really only 2 courses: first, is there a structure in the micrograph you have that has a well know size? RBCs don't count, they change size by a micrometer or more depending on how they're treated (and yes, still look like nice, biconcave discs). If there is, you can calculate magnification and therefore a scale bar from it. But likely not. The other recourse is to have you collaborator take the image again, and this time also take an image of a stage micrometer. Anything else is just guessing. Phil >Hey Guys, > >I'm trying to finish up some figures for a paper. One collaborator has >given me 10X images that appear to have been taken from a standard >digital camera. Can anyone suggest a quick and easy way to add a scale >bar? Unfortunately the journal requires it and I just don't have it. >I have a scale bar for a 40X picture from a different >microscope/experiment, any chance that I can use this? >I'm guessing it is not as simple as my 40X scale bar being 1/4 of the >10X? > >Sorry, but I am clueless about this... any suggestions would be appreciated. > >Thanks, > >Caroline > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Got a little couch potato? Check out fun summer activities for kids. From BJDewe <@t> aol.com Tue Jun 19 08:48:07 2007 From: BJDewe <@t> aol.com (BJDewe@aol.com) Date: Tue Jun 19 08:48:26 2007 Subject: [Histonet] Hard Antibodies! Message-ID: Thanks to everyone who answered! Some very interesting stuff! I too do alot of animal staining and it can be very tricky species to species ;-) Cheers, Lorie ************************************** See what's free at http://www.aol.com. From mcauliff <@t> umdnj.edu Tue Jun 19 09:45:13 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Jun 19 09:45:38 2007 Subject: [Histonet] Formaldehyde: tissue degradation In-Reply-To: <003101c7b255$bdf9af90$0d01a8c0@IBMT41> References: <003101c7b255$bdf9af90$0d01a8c0@IBMT41> Message-ID: <4677EBF9.1000503@umdnj.edu> I would start with the classic texts: *The Microtomist's Vade-Mecum.* Bolles Lee, Arthur. 10th edition, 1937. J.B. Gatenby and T.S. Painter, editors. P. Blakiston's Son and Co., Inc. *The Microtomist's Formulary and Guide.* Gray, Peter; 1954. The Blakiston Co., Inc. *Selected Histochemical and Histopathological Methods.* Thompson, Samuel W. 1966, Charles C. Thomas. *Histological Techniques.* M. Gabe (English edition, transl. E. Blackith and A. Kavoor). Masson, Paris, 1976 Also, how are you going to define "valid scientific evaluation"? Geoff Pankaj Krish Soni wrote: > Dear All > > We are trying to identify some research that studies the degradation of > tissue kept in formaldehyde over long periods of time (over 5 years). > Ultimately we are trying to assess the number of years beyond which the > quality of tissue fixed in formaldehyde would not generally permit valid > scientific evaluation. > > Does anyone know of any relevant research? > > Any help would be very much appreciated. Thank you. > > Krish Soni > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From Barry.R.Rittman <@t> uth.tmc.edu Tue Jun 19 09:51:06 2007 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Tue Jun 19 09:51:07 2007 Subject: [Histonet] teeth In-Reply-To: <002601c7b215$46dc5290$0200a8c0@Janet03b999f> Message-ID: Roberta I have to disagree with both of the suggestions that you have received concerning the processing of teeth. In my hands both these methods produced far inferior results to the use of either Kristensen's Sodium Formate/Formic acid or of disodium EDTA for demineralization of teeth. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janet Maass Sent: Monday, June 18, 2007 8:58 PM To: Roberta Horner Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] teeth Roberta I would recommend 10% trifluoroacetic acid for teeth. The procedure is printed in "The Journal of Histotechnology/ Vol. 25, No. 3, September 2002. Article titled "Processing the Complete Canine Tooth". Janet Maass ----- Original Message ----- From: "Roberta Horner" To: Sent: Monday, June 18, 2007 1:23 PM Subject: [Histonet] teeth I just received some teeth to section can anyone tell me if I can just use any decalcification solution or is there something special to do with teeth? These are from a calf and it has been over 20 years since I took class for histology and in the back of my mind I seem to remember that you need to do something different. Roberta Horner HT, HTL Penn State University Animal Diagnostic Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Barry.R.Rittman <@t> uth.tmc.edu Tue Jun 19 10:01:55 2007 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Tue Jun 19 10:02:02 2007 Subject: [Histonet] Formaldehyde: tissue degradation In-Reply-To: <4677EBF9.1000503@umdnj.edu> Message-ID: Krish Soni Hi. I am not sure precisely the information that you are looking for. There are a plethora of factors that are involved here. For example -are you concerned with loss of microanatomy, of a specific tissue or of components such as amino acids or proteins compared to lipids, compared to carbohydrates? Are you trying to determine the loss of staining or of histochemical or immunochemical reactions? If you could clarify this it would be much appreciated. Thanks Barry Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Geoff McAuliffe Sent: Tuesday, June 19, 2007 9:45 AM To: psoni@post.harvard.edu Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Formaldehyde: tissue degradation I would start with the classic texts: *The Microtomist's Vade-Mecum.* Bolles Lee, Arthur. 10th edition, 1937. J.B. Gatenby and T.S. Painter, editors. P. Blakiston's Son and Co., Inc. *The Microtomist's Formulary and Guide.* Gray, Peter; 1954. The Blakiston Co., Inc. *Selected Histochemical and Histopathological Methods.* Thompson, Samuel W. 1966, Charles C. Thomas. *Histological Techniques.* M. Gabe (English edition, transl. E. Blackith and A. Kavoor). Masson, Paris, 1976 Also, how are you going to define "valid scientific evaluation"? Geoff Pankaj Krish Soni wrote: > Dear All > > We are trying to identify some research that studies the degradation of > tissue kept in formaldehyde over long periods of time (over 5 years). > Ultimately we are trying to assess the number of years beyond which the > quality of tissue fixed in formaldehyde would not generally permit valid > scientific evaluation. > > Does anyone know of any relevant research? > > Any help would be very much appreciated. Thank you. > > Krish Soni > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjr6 <@t> psu.edu Tue Jun 19 11:12:25 2007 From: rjr6 <@t> psu.edu (Roberta Horner) Date: Tue Jun 19 11:12:28 2007 Subject: [Histonet] teeth In-Reply-To: References: Message-ID: We are looking for dentine displaysia (I hope I spelled that right). Roberta -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rittman, Barry R Sent: Monday, June 18, 2007 4:41 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] teeth Roberta Would you let us know what information you need to obtain from the teeth as there are numerous techniques. If you would like to discuss this in detail you are welcome to contact me at 713-500-4134 office or 713 -542 -8108 cellular. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roberta Horner Sent: Monday, June 18, 2007 2:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] teeth I just received some teeth to section can anyone tell me if I can just use any decalcification solution or is there something special to do with teeth? These are from a calf and it has been over 20 years since I took class for histology and in the back of my mind I seem to remember that you need to do something different. Roberta Horner HT, HTL Penn State University Animal Diagnostic Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cornettl <@t> hotmail.com Tue Jun 19 11:13:34 2007 From: cornettl <@t> hotmail.com (Lorraine Cornett) Date: Tue Jun 19 11:13:46 2007 Subject: [Histonet] Re: release of pathology specimens In-Reply-To: <8C98028BA0859F0-180-BBE6@FWM-R06.sysops.aol.com> Message-ID: Unfortunately we found out the hard way that the state of Tennessee has a state statute the defines all pathologic material as infectious waste, therefore it is to be disposed of as biohazardous material. We have adopted a procedure that states no specimens will be released to patients. We do have several exceptions, legal cases, and funeral homes. You can feel free to contact me for the procedure if you are interested. Again please remember this is in the state of TN. I am not sure about other state statutes. Lorraine Cornett, HT (ASCP) Highlands Pathology Blue Ridge Division, Kingsport, TN 423 224-5793 fax 423 224-5349 >From: rsrichmond@aol.com >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Re: release of pathology specimens >Date: Mon, 18 Jun 2007 21:31:43 -0400 > > > Tiffany Price (where?) asks: > >>Does anyone have a policy/procedure for the release of pathology >specimens to patients? (placenta, extremities, etc)<< > >I've never seen a policy written, though there probably needs to be. > >Extremities: Requests for amputated legs for burial in the family cemetery >are fairly common where I practice in rural southern Appalachia. Expect >such requests from highly observant Jews also. Get the help of the family >funeral director - this is a situation they're comfortable with. > >Mastectomy specimens: I've never encountered this situation, but apparently >some women want to bury them. Once again, you need a funeral director. > >Souvenir gallstones: This used to be very common - in one hospital we had >to wash and bottle all the gallstones before we left for the day, in case a >surgeon demanded them - but this practice seems to have been banned by the >infection control people. Souvenir tonsils went the same way. > >Placentas: you don't want to know. Some lay accoucheurs require their >patients to EAT their placentas (rather, their babies' placentas) like >animals do. Several years ago while doing a locum in rural southern Misery >I had an indignant call from a lay midwife in a distant town. Seems she'd >had a delivery with a retained placenta, gotten an OB-GYN to remove it, and >sent it to us for examination. What she was indignant about was that the >hospital had put the placenta in formalin, and in that condition >autoplacentophagy was impossible. > >Reference: Ober, William B. [of blest memory] A modest proposal for >preventing choriocarcinoma among innocent mothers. Obstetrics and >Gynecology 1968;31:866-9. > >Hope you're not reading Histonet over lunch! > >Bob Richmond >Samurai Pathologist >Knoxville TN > > > > >________________________________________________________________________ >AOL now offers free email to everyone. Find out more about what's free >from AOL at AOL.com. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ PC Magazine’s 2007 editors’ choice for best Web mail—award-winning Windows Live Hotmail. http://imagine-windowslive.com/hotmail/?locale=en-us&ocid=TXT_TAGHM_migration_HM_mini_pcmag_0507 From innvx <@t> sbcglobal.net Tue Jun 19 11:55:39 2007 From: innvx <@t> sbcglobal.net (INNOVEX BIOSCIENCES) Date: Tue Jun 19 11:55:43 2007 Subject: [Histonet] Friday Aug 17th, Animal Tissue STAT-IHC staining workshop Message-ID: <46472.38003.qm@web82008.mail.mud.yahoo.com> Animal tissue Stat-IHC workshop will be presented on Friday Aug 17th, 1-4:30 PM in San Francisco at UCSF, Laurel Height campus. For more information, visit innvx.com, click on workshop icon on home page or call 1-800-622-7808 I N N O V E X B I O S C I E N C E S FRIDAY, August 17, 2007 1:00 - 4:30 PM CEU APPROVED WORKSHOP (CEU APPROVED) FRIDAY AUGUST 17, 2007, 1:00-4:30 pm Animal Tissue STAT-IHC staining workshop From primary to microscope under 2 hours PRESENTED by Innovex San Francisco, FRIDAY August 17, 2007, 1:00-4:30 pm LEVEL: Open to all levels This is a comprehensive hands-on wet workshop designated to address the major issues facing the veterinary Histologists that perform animal tissue IHC staining. The topics covered will include eradication of background staining in animal tissues, the techniques for lowering the primary antibody incubation time and lowering the entire staining run for fast turn-around time. Other topics covered will include the selection of appropriate secondary antibodies and co-reagents for IHC staining of animal tissues. This workshop will further address the STAT staining technologies available for background ?Vfree quick IHC staining of animal tissues in less than 2 hours. Other covered topics will include novel amplification technologies for maximizing optimal staining results and eliminating troubleshooting. The application of STAT-IHC reagents and automated stainers will also be explored. This workshop is CEU approved CEU: This workshop is CEU approved for 3 contact hours FEE: This workshop is offered free of charge BENEFICIAL TO: Veterinarian pathologists and histologists, Veterinary IHC Practitioners, Veterinary researchers, Research histotechnologists and research immunohistochemists and to all Cost and time conscious Labs WORKSHOP INSTRUCTORS: Zahra Naser, Ph.D.; M.S, with over 20 years of experience in Immunology, IHC, flow cytometry and Biochemistry and chemistry. Loralei Dewe: Co-Director of Confocal Microscope Core at Shriners Hospital, 10+ years experience with Histology, Immunochemistry and Light and Fluorescent Imaging. SPACE: Space is limited to 22 people for maximum personal attention. Reserve your space as soon as possible. LOCATION: San Francisco, University of California, San Francisco (UCSF), Laurel heights campus Parking: Parking available for a fee. DATE: Friday August 17, 2007, 1:00-4:30 pm TO REGISTER On line, Visit innvx.com, click on Register for workshop on home page. TO REGISTER via Phone: Call us at 1-800-622-7808, Main 510-234-6600. TO REGISTER via fax: Fill out the enclosed registration form and fax it to 510-234-4591. HOTEL INFORMATION: If you require accommodation, please contact Innovex immediately. Also upon registration, hotel and transportation will be emailed or Faxed to you. Contact Innovex at 1-800-622-7808 OR 510-234-6600 Web: innvx.com Disclaimer: Innovex reserves all rights for admission of the participants. From joseph.j.kang <@t> pfizer.com Tue Jun 19 12:20:34 2007 From: joseph.j.kang <@t> pfizer.com (Kang, Joseph J) Date: Tue Jun 19 12:20:42 2007 Subject: [Histonet] baking bone slides for heat retrieval techniques? Message-ID: There have been many shared methods for adhering bone sections to slides and I have probably used them all at one time or another. In my experience it is best to avoid HIER on bone for IHC although I am sure many people do it successfully. I use enzyme digestion instead of heat retriveal for bone IHC (either Proteinase K or Pepsin in most cases). In my hands EIER is more easily controlled and less harsh on tissues than HIER. If I had to use HIER on bone I would choose a more gentle method such as steam rather than boiling. My one cent worth. Patsy Ruegg Patsy, I pulled your message above from Histonet archive and was wondering if you could help me with bone IHC issue. I have been experimenting with bone IHC using different drying times, heat temperatures and using antigen retrieval using the heat and enzyme method. The enzyme method was the only one that kept complete integrity of tissue section, but with no stain. Could you share with me any bone IHC protocol that you use applying the enzyme method that works very well with bone tissue? I really appreciate it. Joe From PMonfils <@t> Lifespan.org Tue Jun 19 14:10:17 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Tue Jun 19 14:10:23 2007 Subject: [Histonet] BrdU kit? Message-ID: <4EBFF65383B74D49995298C4976D1D5E273C9D@LSRIEXCH1.lsmaster.lifespan.org> Can anyone recommend a source of a reliable peroxidase-based anti-BrdU kit? Thanks. From oshel1pe <@t> cmich.edu Tue Jun 19 14:15:54 2007 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Tue Jun 19 14:16:07 2007 Subject: [Histonet] quick and dirty scale bar In-Reply-To: <5C0BED61F529364E86309CADEA63FEF25E6C17@TRFT-EX01.xRothGen.nhs.uk> References: <5C0BED61F529364E86309CADEA63FEF25E6C17@TRFT-EX01.xRothGen.nhs.uk> Message-ID: Terry, They give a needed sense of scale to the subject. This may seem superfluous, but it's valuable. I rather suspect you have a set of neurons tucked away in your cortex that know how big a macrophage fixed with Bouin's is, or a methanol-fixed RBC, etc., so you know what you're looking at and if it's the right size. But. How about a confocal image of the mitochondrial network of a crustacean larva? Or the size range of pollen grains? Or are the pores in that polymer about 1 micron or 10? Obviously you're right that the sizes are "ishy" -- they're ishy even on non-processed samples like a chunk of semiconductor or a ceramic like bone (unless much time and money has been spent properly calibrating the microscopes). I've had more than one dispute about this, where some investigator wants to measure things to 0.01 micrometer or better, and the for-real precision is more like 10%. Still, it's useful to know if the micrograph was taken with a 10X lens a 40X lens or a 100X lens, and if the relay lens was 0.33X or 0.45X or ... get me in the ballpark (sorry, cricket grounds) and I'll have a better shot and figuring out if that little blob is a tiny little one micron green algal cell or a 50 micro green algae -- in, most likey, a completely different kingdom. The meaning really is in your second sentence. You've got lots of known sizes to reference against. Most of us don't. Even in animal tissues, the same kind of cell in a bird isn't going to be as big as in a human or a rat or a lizard or ... . Scale bars become the reference to recognize orders of size and relative sizes. There are few internal standards or references. Phil >Phil, > >And what pray, is their meaning? >One can tell the size of things by reference to known sizes, and >recognize relative sizes. Is this not good enough, even though sizes are >only "ishy"? >If you want to measure things exactly, that's a different matter, but >even then, you only get "ishy" results because of the unknown factor of >shrinking in the processing (and sometimes expansion on the water bath). > >Be that all as it may, I was trying to get a problem solved, which was >to get over the requirement of a scale bar without known magnification >etc. >Nobody surely is going to be in a position to argue unless the scale is >obviously absurd. > >Of course, we don't know (I think) the nature of the photographed >material. It might be something in which size is considered to be >crucial, but I doubt it, as this would have been built into the photos >in the first place. > >Terry (wishing, as usual I had stayed out of it) Marshall > >-----Original Message----- >From: Philip Oshel [mailto:oshel1pe@cmich.edu] >Sent: 19 June 2007 14:46 >To: Marshall Terry Dr, Consultant Histopathologist >Subject: RE: [Histonet] quick and dirty scale bar > >Scale bars may be meaningless to a pathologist, but not to any >microscopist I ever met. Or any biologist or materials scientist working >with micrographs. We certainly don't spend hours in every course >pounding scale bars and plate making into our microscopy majors' heads >because its useless. >Magnification, yeah. Only useful in certain narrow instances. >Not wanting the arrows is more than a tad bizarre, though. What did they >want? Flashing LEDs and hyperlinks to websites? >Phil > >>This is a tad bizarre. I had this problem recently, spending hours >>getting pictures just right for a junior's paper, then finding after >>submission, that they wanted magnification (useless information) and >>didn't want the helpful arrows which would point the uninitiated in the > >>right direction. >>There was nothing for it but to start again. >>Proper pathology journals gave up putting magnifications or scale bars >>years ago (EM pictures excepted). >>It's just the other non-pathology journals which want it, and it's >>totally meaningless, empty information. >> >>I suggest you bullshit. Take a few red cells, call them 6mu, and put on > >>a scale from that. Who's to know the difference? >> >>Terry >> >>-----Original Message----- >>From: histonet-bounces@lists.utsouthwestern.edu >>[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Philip >>Oshel >>Sent: 19 June 2007 14:02 >>To: Histonet@Pathology.swmed.edu >>Subject: Re: [Histonet] quick and dirty scale bar >> >>Caroline, >> >>No, you can't use the 40X scale bar, and yes, it's not that simple. >>Even if the image with the 40X scale bar were taken on the same >>microscope with the same camera and the same settings, it wouldn't be >>in an exact 1:4 relation with the 10X objective. >>Further, you don't know what if any zoom your collaborator used. If the > >>image was taken with a standard digital camera (e.g., a Nikon >>Coolpix) through an eyepiece adapter, there is often vignetting around >>the edges. This prompts people to use the zoom function to eliminate >>this. Which of course means the image isn't "10X". >>Which it may not have been anyway, depending on the lens in the >>eyepiece adapter. >>The only way to get images with known scale bars is to take a >>micrograph of a stage micrometer **without changing the camera**, >>especially the zoom, for each objective used, *at the time the >micrographs are taken*. >>Otherwise it is nearly impossible to replicate the zoom used. >>Calculations/measurements based on field of view aren't trustworthy for > >>the same reasons -- you don't know the true field of view. >>If no zoom was used, then it may be possible to take a photo of a stage > >>micrometer with the same objective, etc., and be OK. But I wouldn't >>trust it. >> >>Given what you have, there are really only 2 courses: first, is there a > >>structure in the micrograph you have that has a well know size? >>RBCs don't count, they change size by a micrometer or more depending on > >>how they're treated (and yes, still look like nice, biconcave discs). >>If there is, you can calculate magnification and therefore a scale bar >>from it. >>But likely not. The other recourse is to have you collaborator take the > >>image again, and this time also take an image of a stage micrometer. >>Anything else is just guessing. >> >>Phil >> >>>Hey Guys, >>> >>>I'm trying to finish up some figures for a paper. One collaborator >>>has >> >>>given me 10X images that appear to have been taken from a standard >>>digital camera. Can anyone suggest a quick and easy way to add a >>>scale >> >>>bar? Unfortunately the journal requires it and I just don't have it. >>>I have a scale bar for a 40X picture from a different >>>microscope/experiment, any chance that I can use this? >>>I'm guessing it is not as simple as my 40X scale bar being 1/4 of the >>>10X? >>> >>>Sorry, but I am clueless about this... any suggestions would be >>appreciated. >>> >>>Thanks, >>> >>>Caroline >>> >>> >>> >>>_______________________________________________ >>>Histonet mailing list >>>Histonet@lists.utsouthwestern.edu >>>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >>-- >>Philip Oshel >>Microscopy Facility Supervisor >>Biology Department >>024C Brooks Hall >>Central Michigan University >>Mt. Pleasant, MI 48859 >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Melissa.Gonzalez <@t> cellgenesys.com Tue Jun 19 16:44:15 2007 From: Melissa.Gonzalez <@t> cellgenesys.com (Melissa Gonzalez) Date: Tue Jun 19 16:44:28 2007 Subject: [Histonet] pRb IHC In-Reply-To: <20070619171357.71C2027F1CE@mail.cellgenesys.com> References: <20070619171357.71C2027F1CE@mail.cellgenesys.com> Message-ID: <2884B897182A1D438C7BA24B9A8F94A20660BB@hqsvr01mail.cgi.com> Hi all, I was wondering for those of you out there familiar with staining for Retinoblastoma (Rb) defects what products do you use and how clean are the results? I tend to get variable cytoplasmic staining (it's supposed to be nuclear only) in the various tissue microarrays we're looking at. [lower ab [] also weakens nuclear expression] This may be perfectly normal, but all the published images show only the very sharp, clean nuclear staining. I also do not frequently see the "internal positive control" staining of endothelial cells. TIA, your input is always appreciated, Melissa From anh2006 <@t> med.cornell.edu Wed Jun 20 00:27:28 2007 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Wed Jun 20 00:27:33 2007 Subject: [Histonet] Osteocalcin Message-ID: Dear All, Is anyone using osteocalcin for detection of osteoblasts in MOUSE tissue sections? If so, can you give me recommendations for your antibody and protocol? I bought an Ab recommended in the archives from Biomedical Technologies, Inc. and it giving me nothing but heinous background (it's a goal polyclonal SERUM). Thank you in advance, Andrea -- From c.weaver <@t> vla.defra.gsi.gov.uk Wed Jun 20 04:26:30 2007 From: c.weaver <@t> vla.defra.gsi.gov.uk (Weaver, Colin) Date: Wed Jun 20 04:26:36 2007 Subject: [Histonet] Staining for heavy metals Message-ID: <7A885E8FE1C71C488D974EC601FAA690017399B3@vla-exchn1.cvlnt.vla.gov.uk> Hi all - At present we use at our lab a Rubeanic acid stain for copper in tissue, have also tried Rhodamine but have never been totally convinced by either. I have seen references to a Timm's stain for heavy metals. Does anyone use it?, is it any good? Would anyone be prepared to send me a protocol. Thanks Colin Veterinary Laboratories Agency (VLA) This email and any attachments is intended for the named recipient only. If you have received it in error you have no authority to use, disclose, store or copy any of its contents and you should destroy it and inform the sender. Whilst this email and associated attachments will have been checked for known viruses whilst within VLA systems we can accept no responsibility once it has left our systems. Communications on VLA's computer systems may be monitored and/or recorded to secure the effective operation of the system and for other lawful purposes. From rjbuesa <@t> yahoo.com Wed Jun 20 07:04:21 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jun 20 07:04:26 2007 Subject: [Histonet] Staining for heavy metals In-Reply-To: <7A885E8FE1C71C488D974EC601FAA690017399B3@vla-exchn1.cvlnt.vla.gov.uk> Message-ID: <960183.17915.qm@web61217.mail.yahoo.com> Colin: It is not that it is "any good", it is just the best for copper! Under separate cover I am sending you the protocol. Ren? J. "Weaver, Colin" wrote: Hi all - At present we use at our lab a Rubeanic acid stain for copper in tissue, have also tried Rhodamine but have never been totally convinced by either. I have seen references to a Timm's stain for heavy metals. Does anyone use it?, is it any good? Would anyone be prepared to send me a protocol. Thanks Colin Veterinary Laboratories Agency (VLA) This email and any attachments is intended for the named recipient only. If you have received it in error you have no authority to use, disclose, store or copy any of its contents and you should destroy it and inform the sender. Whilst this email and associated attachments will have been checked for known viruses whilst within VLA systems we can accept no responsibility once it has left our systems. Communications on VLA's computer systems may be monitored and/or recorded to secure the effective operation of the system and for other lawful purposes. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Don't pick lemons. See all the new 2007 cars at Yahoo! Autos. From HornHV <@t> archildrens.org Wed Jun 20 07:45:57 2007 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Jun 20 07:46:12 2007 Subject: [Histonet] Staining for heavy metals In-Reply-To: <960183.17915.qm@web61217.mail.yahoo.com> References: <7A885E8FE1C71C488D974EC601FAA690017399B3@vla-exchn1.cvlnt.vla.gov.uk> <960183.17915.qm@web61217.mail.yahoo.com> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB71D5@EMAIL.archildrens.org> Colin we use a microwave copper stain that is excellent from American Master Tech Scientific. It's easy and quick and works wonderfully. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- "Weaver, Colin" wrote: Hi all - At present we use at our lab a Rubeanic acid stain for copper in tissue, have also tried Rhodamine but have never been totally convinced by either. I have seen references to a Timm's stain for heavy metals. Does anyone use it?, is it any good? Would anyone be prepared to send me a protocol. Thanks Colin Veterinary Laboratories Agency (VLA) This email and any attachments is intended for the named recipient only. If you have received it in error you have no authority to use, disclose, store or copy any of its contents and you should destroy it and inform the sender. Whilst this email and associated attachments will have been checked for known viruses whilst within VLA systems we can accept no responsibility once it has left our systems. Communications on VLA's computer systems may be monitored and/or recorded to secure the effective operation of the system and for other lawful purposes. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Don't pick lemons. See all the new 2007 cars at Yahoo! Autos. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From Kim.Shields <@t> viha.ca Wed Jun 20 08:21:08 2007 From: Kim.Shields <@t> viha.ca (Shields, Kim) Date: Wed Jun 20 08:21:14 2007 Subject: [Histonet] Tissue Tek Prisma opinions Message-ID: Is anyone using the Tissue Tek Prisma automated combo slide stainer & coverslipper? Please post any opinions or thoughts. Thanks in advance. Kim Shields VIHA Victoria BC, Canada From Rcartun <@t> harthosp.org Wed Jun 20 09:06:38 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Jun 20 09:06:53 2007 Subject: [Histonet] S-100 antibody Message-ID: <4678FC2E0200007700006770@gwmail.harthosp.org> For years we have used polyclonal antibodies for the immunohistochemical detection of S-100 protein in routinely processed human tissue. Does anyone have a recommendation for a good monoclonal antibody to S-100? Thank you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From sonya.martin <@t> soton.ac.uk Wed Jun 20 09:37:40 2007 From: sonya.martin <@t> soton.ac.uk (Martin S.) Date: Wed Jun 20 09:38:02 2007 Subject: [Histonet] Using antiserum for IHC Message-ID: <71437982F5B13A4D9A5B2669BDB89EE40DDCF0C0@ISS-CL-EX-V1.soton.ac.uk> Hi All, Has anyone got some tips for using antiserum for immunolabelling frozen tumour sections? I have some anti asialo GM1 (rabbit polyclonal, reacts with moues NK cells). There are a couple of reports of it being used to label frozen sections but they dont give any details. I have tried it at 1/10, 1/50, 1/100, 1/500, 1/1000 and 1/5000 dilution but for all dilutions there just seems to be background staining of the whole tissue (it does get progressively better with increasing dilution but even at 1/5000 it wouldnt be good enough to sepatae positive staining from the background. I used a biotinylated secondary (goat anti-rabbit from Vector, which works well with another rabbit primary antibody and is clean when used without primary) and blocked with 5% normal goat serum before the primary incubation and then just with PBS in between antibodies. I used Vectors ABC kit with DAB for detection. I havent used antiserum before and was wondering if there are any special considerations or blocking steps that I should try. The secondary is only anti-rabbit IgG wheras the antiserum is polyclonal so maybe thats the reason - however in one of the previous reports they used an anti-rabbit IgG as secondary........... Any suggestions welcomed! Sonya From sonya.martin <@t> soton.ac.uk Wed Jun 20 09:44:49 2007 From: sonya.martin <@t> soton.ac.uk (Martin S.) Date: Wed Jun 20 09:45:30 2007 Subject: [Histonet] NK cells in mouse frozens Message-ID: <71437982F5B13A4D9A5B2669BDB89EE40DDCF0C1@ISS-CL-EX-V1.soton.ac.uk> Hi All, In addition to my other question today (about labelling with antiserum) - has anyone successfully labelled NK cells in frozen sections of mouse tissues? If so which tissue and which antibodies did you use? Thanks Sonya From brett_connolly <@t> merck.com Wed Jun 20 10:32:26 2007 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Wed Jun 20 10:33:01 2007 Subject: [Histonet] Processing frozen tissue to paraffin Message-ID: <63EA0607835FBA4689CEA9EA8B4826923E96A9@usctmx1141.merck.com> Hi all, I have some fresh frozen tissue at -80C I would like to process to paraffin blocks and then run some subsequent IHC. Can I just plop it into 10%NBF and process as usual? Thx, Brett Brett M. Connolly, Ph.D. Research Fellow MRL, Imaging Research Merck & Co., Inc. WP-44K PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com ------------------------------------------------------------------------------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------------ From brett_connolly <@t> merck.com Wed Jun 20 10:37:20 2007 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Wed Jun 20 10:37:43 2007 Subject: [Histonet] microglia in mouse brains Message-ID: <63EA0607835FBA4689CEA9EA8B4826923E96AE@usctmx1141.merck.com> A colleague is looking for an antibody & protocol for staining microglia in mice. Any suggestions? Thx, Brett Brett M. Connolly, Ph.D. Research Fellow MRL, Imaging Research Merck & Co., Inc. WP-44K PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com ------------------------------------------------------------------------------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------------ From hancockestates <@t> yahoo.com Wed Jun 20 10:53:21 2007 From: hancockestates <@t> yahoo.com (MaryAnn Hancock) Date: Wed Jun 20 10:53:27 2007 Subject: [Histonet] BONE MARROW DECAL Message-ID: <20070620155321.72871.qmail@web51601.mail.re2.yahoo.com> I am interested to know what Decal fluid is being used for Bone Marrow Core Biopsies and how long you fix them for. Thanks MaryAnn --------------------------------- Sick sense of humor? Visit Yahoo! TV's Comedy with an Edge to see what's on, when. From asachau <@t> titanmed.com Wed Jun 20 10:57:28 2007 From: asachau <@t> titanmed.com (April Sachau) Date: Wed Jun 20 11:06:11 2007 Subject: [Histonet] Available Histologist In-Reply-To: <20070620155321.72871.qmail@web51601.mail.re2.yahoo.com> Message-ID: <7E3ACD48BA6E26408F3188FBF08693F79E1F57@titansbs1.corp.titanmed.com> Hello Histonetters! I have an awesome Histologist with over 30 years of experience that will be finishing an assignment for me this week. He has done a wonderful job for this hospital and will be available for another assignment as of 07/25/07. Do you have any needs that may be a match? Let me know if you would like to review his profile. April Sachau Titan Medical Group Staff Supervisor Phone (866) 332-9600 Ext. 1023 Fax (402) 332-5181 asachau@titanmed.com see us on the web at www.titanmed.com From liz <@t> premierlab.com Wed Jun 20 11:24:42 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Jun 20 11:25:00 2007 Subject: [Histonet] microglia in mouse brains In-Reply-To: <5F19831F1377478F981F7539798493A0@PremierLab.local> References: <5F19831F1377478F981F7539798493A0@PremierLab.local> Message-ID: Brett I'm not positive but I think that you might be able to use F4/80 as = microglia marker in mouse brain. Liz=20 Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com =20 Ship to Address: =20 Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu = [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of = Connolly, Brett M Sent: Wednesday, June 20, 2007 9:46 AM To: histonet@lists.utsouthwestern.edu; ihcrg@googlegroups.com Subject: [Histonet] microglia in mouse brains A colleague is looking for an antibody & protocol for staining microglia = in mice. Any suggestions? Thx, Brett Brett M. Connolly, Ph.D. Research Fellow MRL, Imaging Research Merck & Co., Inc. WP-44K PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com -------------------------------------------------------------------------= ----- Notice: This e-mail message, together with any attachments, contains = information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, = New Jersey, USA 08889), and/or its affiliates (which may be known = outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD = and in Japan, as Banyu - direct contact information for affiliates is = available at http://www.merck.com/contact/contacts.html) that may be = confidential, proprietary copyrighted and/or legally privileged. It is = intended solely for the use of the individual or entity named on this = message. If you are not the intended recipient, and have received this = message in error, please notify us immediately by reply e-mail and then = delete it from your system. -------------------------------------------------------------------------= ----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG Free Edition.=20 Version: 7.5.472 / Virus Database: 269.9.1/857 - Release Date: 6/20/2007 = 2:18 PM =20 No virus found in this outgoing message. Checked by AVG Free Edition.=20 Version: 7.5.472 / Virus Database: 269.9.1/857 - Release Date: 6/20/2007 = 2:18 PM =20 From PMonfils <@t> Lifespan.org Wed Jun 20 12:02:00 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Jun 20 12:02:08 2007 Subject: [Histonet] Processing frozen tissue to paraffin In-Reply-To: <63EA0607835FBA4689CEA9EA8B4826923E96A9@usctmx1141.merck.com> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273C9F@LSRIEXCH1.lsmaster.lifespan.org> I have done this a number of times, with good results. No artifact should be introduced by the thawing process, if the tissue is thawed in formalin. Of course, if artifacts were introduced in the process of freezing, because of freezing too slow or other factors, those artifacts will remain. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Connolly, Brett M > Sent: Wednesday, June 20, 2007 11:32 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Processing frozen tissue to paraffin > > Hi all, > I have some fresh frozen tissue at -80C I would like to process to > paraffin blocks and then run some subsequent IHC. > > Can I just plop it into 10%NBF and process as usual? > > Thx, > Brett > > Brett M. Connolly, Ph.D. > Research Fellow > MRL, Imaging Research > Merck & Co., Inc. > WP-44K > PO Box 4 > West Point, PA 19486 > PH 215-652-2501 > fax. 215-993-6803 > e-mail. brett_connolly@merck.com > > > ------------------------------------------------------------------------------ > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, > New Jersey, USA 08889), and/or its affiliates (which may be known > outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD > and in Japan, as Banyu - direct contact information for affiliates is > available at http://www.merck.com/contact/contacts.html) that may be > confidential, proprietary copyrighted and/or legally privileged. It is > intended solely for the use of the individual or entity named on this > message. If you are not the intended recipient, and have received this > message in error, please notify us immediately by reply e-mail and then > delete it from your system. > > ------------------------------------------------------------------------------ > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From cbass <@t> bidmc.harvard.edu Wed Jun 20 12:40:04 2007 From: cbass <@t> bidmc.harvard.edu (cbass@bidmc.harvard.edu) Date: Wed Jun 20 12:40:16 2007 Subject: [Histonet] non-mouse anti-flag antibody Message-ID: <4B5700BE37215344A81185F131A848C261EB80@EVS6.its.caregroup.org> Hello, I was hoping someone could suggest a good anti-flag antibody for use in mouse brain. A lot of anti-flags are monoclonal, which is not very good for mouse tissue obviously. Does anyone have a suggestion? The amino acid sequence of the flag is DYKDDDDK. A FITC tag would be helpful as well, but right now just having a non-mouse antibody would be great. Also, has anyone tried the mouse-on-mouse kits in the brain or have suggestions for going this route? Thanks! Caroline From vazquezr <@t> ohsu.edu Wed Jun 20 12:47:21 2007 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Wed Jun 20 12:47:51 2007 Subject: [Histonet] accreditatation question Message-ID: I currently work at Dermatology Surgery at OHSU as a Mohs Tech. Robyn Vazquez is my coworker. I am writing to inquire about the ASCP eligibility requirements for the HTL exam. Would someone know how I could find out, since the ASCP will not verify eligibility until you apply for the exam and pay the fee ($175)? To be eligible for the exam under the Route 2 method you are required to have BS degree and 45 credits of biology and chemistry. I have that covered. Also, they state that you need one year full time acceptable experience in a histopathology lab. "This year of experience must be under the supervision of a pathologist (certified by the American Board of Pathology in Anatomic Pathology) or an appropriately board certified medical scientist." We currently work with the Mohs surgeons, however, the laboratory functions under the license of our Dermatopathologist. Dr. Cliff White is board certified in Dermatology and has a Special Competence Certification in Dermatopathology from the American Boards of Dermatology and Pathology. Now is his accreditation enough for me? That is the question that I am looking for. I am interested in finding someone who has been accepted to take the exam with the same kind of background. Thank you for your time, Maria M. Samaan Med Tech II Derm/Surgical Phone: 503-494-4658 Fax: 503-418-9041 samaanm@ohsu.edu From jennifer.harvey <@t> Vanderbilt.Edu Wed Jun 20 12:47:50 2007 From: jennifer.harvey <@t> Vanderbilt.Edu (Harvey, Jennifer Lynn) Date: Wed Jun 20 12:47:55 2007 Subject: [Histonet] frozen sections on monkey eye Message-ID: Histonet: I thought years of bone work I had done some of the most challenging histology. We move to Nashville and I took a job in eye research. Was I crazy??? This is worse. I need to cut frozen sections on 4%PFA fixed monkey eyes? Any ideas?? Thanks!!!! Jennifer Harvey Vanderbilt Eye Institute RM 8105 MCE North Tower 1215 21st Ave. South Nashville, TN 37232-8808 Phone: 615-936-1486 From sbreeden <@t> nmda.nmsu.edu Wed Jun 20 12:59:33 2007 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Jun 20 12:59:42 2007 Subject: [Histonet] Countertop Color Message-ID: <4D14F0FC9316DD41972D5F03C070908B8F4507@nmdamailsvr.nmda.ad.nmsu.edu> Okay - we're still planning this new lab... what COLOR would you choose for countertops? I'm thinkin' BLACK. I don't even know if I have a choice, but I'm trying to run ahead of the pack... Thanks! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From lpaveli1 <@t> hurleymc.com Wed Jun 20 13:31:48 2007 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Wed Jun 20 13:32:23 2007 Subject: [Histonet] Small cell carcinoma Message-ID: <46793A54020000EE000156D9@smtp-gw.hurleymc.com> Hello, I am curious to see what other institutions are using to diagnose small cell carcinoma in human formalin fixed, paraffin tissue. I have run into some literature that states that synaptophysin will stain positive 90% of the time, and in other articles will stain positive only 50% of the time. Are there other antibodies that are more specific that you have found work better?? Awaiting your knowledge and experience. Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810-257-9948 fax: 810-762-7082 From dpahisto <@t> yahoo.com Wed Jun 20 13:32:58 2007 From: dpahisto <@t> yahoo.com (Cindy DuBois) Date: Wed Jun 20 13:33:05 2007 Subject: [Histonet] Sakura Stainer Equipment Message-ID: <291688.6046.qm@web33411.mail.mud.yahoo.com> We have a Sakura Tissue-Tek Stainer (model DRS601). We are trying to locate another set of racks and staining dishes. Does anyone have a set they would like to sell? Is there a good place to find used equipment such as this? Buying it new is quite pricey. Cindy Dubois Integrated Pathology Services (209)477-4432 --------------------------------- Get the free Yahoo! toolbar and rest assured with the added security of spyware protection. From godsgalnow <@t> aol.com Wed Jun 20 13:56:33 2007 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Wed Jun 20 13:57:03 2007 Subject: [Histonet] Small cell carcinoma In-Reply-To: <46793A54020000EE000156D9@smtp-gw.hurleymc.com> References: <46793A54020000EE000156D9@smtp-gw.hurleymc.com> Message-ID: <8C98183DAD40480-16CC-12D09@FWM-D36.sysops.aol.com> We do not do just the synapto, but also?chromogranin and NSE. Roxanne Soto HT(ASCP)QIHC Lab Supervisor -----Original Message----- From: Lynette Pavelich To: Histonet@lists.utsouthwestern.edu Sent: Wed, 20 Jun 2007 2:31 pm Subject: [Histonet] Small cell carcinoma Hello, am curious to see what other institutions are using to diagnose small ell carcinoma in human formalin fixed, paraffin tissue. I have run into some literature that states that synaptophysin will tain positive 90% of the time, and in other articles will stain ositive only 50% of the time. re there other antibodies that are more specific that you have found ork better?? Awaiting your knowledge and experience. Lynette Pavelich, HT(ASCP) istology Supervisor urley Medical Center ne Hurley Plaza lint, MI 48503 ph: 810-257-9948 ax: 810-762-7082 _______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From LSebree <@t> uwhealth.org Wed Jun 20 14:00:48 2007 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Wed Jun 20 14:00:53 2007 Subject: [Histonet] Small cell carcinoma In-Reply-To: <46793A54020000EE000156D9@smtp-gw.hurleymc.com> Message-ID: Try going to Immunquery for some nice algorithm data. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Wednesday, June 20, 2007 1:32 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Small cell carcinoma Hello, I am curious to see what other institutions are using to diagnose small cell carcinoma in human formalin fixed, paraffin tissue. I have run into some literature that states that synaptophysin will stain positive 90% of the time, and in other articles will stain positive only 50% of the time. Are there other antibodies that are more specific that you have found work better?? Awaiting your knowledge and experience. Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810-257-9948 fax: 810-762-7082 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpaveli1 <@t> hurleymc.com Wed Jun 20 14:03:08 2007 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Wed Jun 20 14:03:40 2007 Subject: [Histonet] Small cell carcinoma Message-ID: <467941AC020000EE000156E2@smtp-gw.hurleymc.com> Sorry if there was any confusion, our docs usually order a sort of panel to decifer small cell v/s non-small cell. They will order AE1/AE3, CK7, CK20, TTF-1, NSE, Synaptophysin, and Chromogranin. Do any of you have any other suggestions for antibodies that might help to pin-point small cell carcinoma? thanks again, Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810-257-9948 fax: 810-762-7082 >>> 06/20/07 2:56 PM >>> We do not do just the synapto, but also chromogranin and NSE. Roxanne Soto HT(ASCP)QIHC Lab Supervisor -----Original Message----- From: Lynette Pavelich To: Histonet@lists.utsouthwestern.edu Sent: Wed, 20 Jun 2007 2:31 pm Subject: [Histonet] Small cell carcinoma Hello, am curious to see what other institutions are using to diagnose small ell carcinoma in human formalin fixed, paraffin tissue. I have run into some literature that states that synaptophysin will tain positive 90% of the time, and in other articles will stain ositive only 50% of the time. re there other antibodies that are more specific that you have found ork better?? Awaiting your knowledge and experience. Lynette Pavelich, HT(ASCP) istology Supervisor urley Medical Center ne Hurley Plaza lint, MI 48503 ph: 810-257-9948 ax: 810-762-7082 _______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From TMSykora <@t> gundluth.org Wed Jun 20 14:10:53 2007 From: TMSykora <@t> gundluth.org (TMSykora@gundluth.org) Date: Wed Jun 20 14:10:59 2007 Subject: [Histonet] Evaluating AP system Message-ID: Hi all, we our pathology department is looking to upgrade our current computer AP system. We have narrowed the search down to four vendors. They are: Cerner DHT- CoPathPlus Impac Medical Systems - PowerPath Psyche Systems Corp - WindoPath SCC Soft Computer - SoftPath Any feedback is welcome. Positive or negative Thanks for your help, Theresa Sykora Histology Laboratory Gundersen Lutheran Medical Center 1900 South Ave. HO4-008 La Crosse, WI 54601 tmsykora@gundluth.org 608-775-3139 From rjbuesa <@t> yahoo.com Wed Jun 20 14:42:15 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jun 20 14:42:18 2007 Subject: [Histonet] Processing frozen tissue to paraffin In-Reply-To: <63EA0607835FBA4689CEA9EA8B4826923E96A9@usctmx1141.merck.com> Message-ID: <842614.37963.qm@web61219.mail.yahoo.com> After thawing, of course! Ren? J. "Connolly, Brett M" wrote: Hi all, I have some fresh frozen tissue at -80C I would like to process to paraffin blocks and then run some subsequent IHC. Can I just plop it into 10%NBF and process as usual? Thx, Brett Brett M. Connolly, Ph.D. Research Fellow MRL, Imaging Research Merck & Co., Inc. WP-44K PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com ------------------------------------------------------------------------------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- We won't tell. Get more on shows you hate to love (and love to hate): Yahoo! TV's Guilty Pleasures list. From rjbuesa <@t> yahoo.com Wed Jun 20 14:43:09 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jun 20 14:43:14 2007 Subject: [Histonet] BONE MARROW DECAL In-Reply-To: <20070620155321.72871.qmail@web51601.mail.re2.yahoo.com> Message-ID: <360168.52633.qm@web61213.mail.yahoo.com> EDTA at pH7; usually overnight. Ren? J. MaryAnn Hancock wrote: I am interested to know what Decal fluid is being used for Bone Marrow Core Biopsies and how long you fix them for. Thanks MaryAnn --------------------------------- Sick sense of humor? Visit Yahoo! TV's Comedy with an Edge to see what's on, when. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get the Yahoo! toolbar and be alerted to new email wherever you're surfing. From sbreeden <@t> nmda.nmsu.edu Wed Jun 20 14:43:29 2007 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Jun 20 14:43:35 2007 Subject: [Histonet] Counter Color Message-ID: <4D14F0FC9316DD41972D5F03C070908B8F450B@nmdamailsvr.nmda.ad.nmsu.edu> Thanks everybody for the ideas. I think the solid-surface-hematoxylin-paraffin-carbol-fuchsin-tissue-biopsy-colored countertops I've picked out will work just fine. Now if I could only decide what color clothes to wear to work that perform the same camouflage... I'll be lucky to even see this lab before I retire in 3.5 years! It's been in the works for 3 years so far. I'll tell you, though, I sure am glad I took that seminar at NSH a couple years ago about planning a new lab space - that's been a PRICELESS resource when working with architects, not to mention the input from Histonet. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From carl.hobbs <@t> kcl.ac.uk Wed Jun 20 14:53:36 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Wed Jun 20 14:53:58 2007 Subject: [Histonet] re: mouse S100 Message-ID: <001e01c7b374$b0ba5c10$4001a8c0@carlba65530bda> I have a sample of mouse anti-S100 ( clone 4B3) from Abcam that I got a year ago: I keep it as a 1/10 diluted 4C stock ; worked beautifully on rat/mouse FFPW sections, after HIER, today.......( final dilution factor ~1/3000) As it is an "anti-human" S100a/b, every reason to think that it will be as good on human equivilent sections. I used a std stABCpxDAB. Abcam no longer sell it. However, it is still available from other sources. Have a look at pic here( image gallery) http://www.immunoportal.com/index.php Carl From carl.hobbs <@t> kcl.ac.uk Wed Jun 20 14:57:43 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Wed Jun 20 14:57:57 2007 Subject: [Histonet] re: microglia in mouse brains Message-ID: <002201c7b375$443fdcd0$4001a8c0@carlba65530bda> Well, if you are talking FFPW sections, after HIER, please see here for examples: http://www.immunoportal.com/index.php Carlos From jmahoney <@t> alegent.org Wed Jun 20 15:04:42 2007 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Wed Jun 20 15:04:53 2007 Subject: [Histonet] Evaluating AP system In-Reply-To: References: Message-ID: <4679420A0200003C00011776@gwia.alegent.org> I'm a Cerner Mellenium user and builder and love the ability I have to make they system work for my lab. Jan >>> 06/20/2007 2:10:53 PM >>> Hi all, we our pathology department is looking to upgrade our current computer AP system. We have narrowed the search down to four vendors. They are: Cerner DHT- CoPathPlus Impac Medical Systems - PowerPath Psyche Systems Corp - WindoPath SCC Soft Computer - SoftPath Any feedback is welcome. Positive or negative Thanks for your help, Theresa Sykora Histology Laboratory Gundersen Lutheran Medical Center 1900 South Ave. HO4-008 La Crosse, WI 54601 tmsykora@gundluth.org 608-775-3139 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Wed Jun 20 15:12:03 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Jun 20 15:12:20 2007 Subject: [Histonet] Small cell carcinoma In-Reply-To: <46793A54020000EE000156D9@smtp-gw.hurleymc.com> References: <46793A54020000EE000156D9@smtp-gw.hurleymc.com> Message-ID: <467951D302000077000067A7@gwmail.harthosp.org> A good H&E and, if necessary, CK8/18 (delicate, sometimes punctate cytoplasmic reactivity) and chromogranin (rare, granular cytoplasmic staining). Synaptophysin is not one of my favorite antibodies due to specificity issues. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Lynette Pavelich" 06/20/07 2:31 PM >>> Hello, I am curious to see what other institutions are using to diagnose small cell carcinoma in human formalin fixed, paraffin tissue. I have run into some literature that states that synaptophysin will stain positive 90% of the time, and in other articles will stain positive only 50% of the time. Are there other antibodies that are more specific that you have found work better?? Awaiting your knowledge and experience. Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810-257-9948 fax: 810-762-7082 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From mpence <@t> grhs.net Wed Jun 20 15:18:28 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Jun 20 15:18:41 2007 Subject: [Histonet] Evaluating AP system In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C641@IS-E2K3.grhs.net> Although the people at Cerner are not the best to work with, CoPath is the "Gold Standard" when it comes to AP systems. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of TMSykora@gundluth.org Sent: Wednesday, June 20, 2007 2:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Evaluating AP system Hi all, we our pathology department is looking to upgrade our current computer AP system. We have narrowed the search down to four vendors. They are: Cerner DHT- CoPathPlus Impac Medical Systems - PowerPath Psyche Systems Corp - WindoPath SCC Soft Computer - SoftPath Any feedback is welcome. Positive or negative Thanks for your help, Theresa Sykora Histology Laboratory Gundersen Lutheran Medical Center 1900 South Ave. HO4-008 La Crosse, WI 54601 tmsykora@gundluth.org 608-775-3139 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJasper <@t> smdc.org Wed Jun 20 15:25:18 2007 From: TJasper <@t> smdc.org (Jasper, Thomas G.) Date: Wed Jun 20 15:25:36 2007 Subject: [Histonet] Evaluating AP system In-Reply-To: Message-ID: <1A9F2A6C5762524799A816F1F09744CF0143A5E1@SCREECH.ntcampus.smdc.org> Hi Theresa, We were users of PowerPath (Impath) and very happy with the system. Unfortunately, we've had to give it up for the sake of the rest of Laboratory Services. The upper administration here wants everyone (all labs) on the same system. Great idea, the only problem now is the AP module for this integrated system isn't even close to as good as PowerPath was. My vote would definitely be for PowerPath. If you wish, I can discuss further off this list server, we are now going through a very difficult transition having given up PowerPath. Tom J. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of TMSykora@gundluth.org Sent: Wednesday, June 20, 2007 2:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Evaluating AP system Hi all, we our pathology department is looking to upgrade our current computer AP system. We have narrowed the search down to four vendors. They are: Cerner DHT- CoPathPlus Impac Medical Systems - PowerPath Psyche Systems Corp - WindoPath SCC Soft Computer - SoftPath Any feedback is welcome. Positive or negative Thanks for your help, Theresa Sykora Histology Laboratory Gundersen Lutheran Medical Center 1900 South Ave. HO4-008 La Crosse, WI 54601 tmsykora@gundluth.org 608-775-3139 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From vic <@t> vetmed.wsu.edu Wed Jun 20 15:33:23 2007 From: vic <@t> vetmed.wsu.edu (Leyva-Grado, Victor) Date: Wed Jun 20 15:33:28 2007 Subject: [Histonet] RE: microglia in mouse brains In-Reply-To: References: Message-ID: <34EFB08480241347BEBE1F095ABB96F4207709@cvm36.vetmed.wsu.edu> Hi Brett, As Liz mentioned, F4/80 (a pan macrophage marker) will work. I've been using Serotec's in frozen sections and it works fine for DAB staining. Not very strong signal though when I used for fluorescence double labeling. Some people also use anti-CD11b. I used it a couple times but in my hands didn't work. Victor H Leyva DVM Department of VCAPP Washington State University From gcallis <@t> montana.edu Wed Jun 20 15:54:55 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed Jun 20 15:55:08 2007 Subject: [Histonet] Countertop Color In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B8F4507@nmdamailsvr.nmda.ad .nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B8F4507@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <6.0.0.22.1.20070620145005.01b6adb8@gemini.msu.montana.edu> Black is best for spilled/splashed stains that seem to turn up willy nilly in histolabs. All our labs have black countertops, and some are chemically resistant too. At 11:59 AM 6/20/2007, you wrote: >Okay - we're still planning this new lab... what COLOR would you choose >for countertops? I'm thinkin' BLACK. I don't even know if I have a >choice, but I'm trying to run ahead of the pack... Thanks! Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From PMonfils <@t> Lifespan.org Wed Jun 20 16:25:29 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Jun 20 16:25:34 2007 Subject: [Histonet] more on BrdU Message-ID: <4EBFF65383B74D49995298C4976D1D5E273CA2@LSRIEXCH1.lsmaster.lifespan.org> Thanks to all who responded to my querry concerning BrdU kits. Buying a separate anti-BrdU antibody instead of a kit sounds like a good idea. Could the folks who mentioned the Dako antibody please let me know which product you are using and where you get it? Someone mentioned that they use a separate antibody because the kits are too expensive. However, I called Dako and the only BrdU antibody they seem to have costs $451 for 1 ml, which is almost twice the price of the complete kit from InVitrogen. Thanks. From liz <@t> premierlab.com Wed Jun 20 16:42:20 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Jun 20 16:42:24 2007 Subject: [Histonet] picro sirius red Message-ID: Hello everyone =20 We are running some picro sirius red for image analysis and were running = into a bit of a problem. We are staining rat heart, the collagen = staining is quite nice but some of the muscle is staining a tad bit = orangish rather than a really nice yellow. Which is not a problem when = you look under the microscope but once the images are captured for = analysis thats were we run into problems. We have tried making up = fresh staining solution, etc. differenciating more in the acetic acid = but the muscle is stil a bit orange in color. Any help would be = appreciated. =20 =20 Thanks in advance =20 Liz =20 Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 HYPERLINK "mailto:liz@premierlab.com"liz@premierlab.com HYPERLINK "http://www.premierlab.com/"www.premierlab.com =20 Ship to Address: =20 Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 =20 No virus found in this outgoing message. Checked by AVG Free Edition.=20 Version: 7.5.472 / Virus Database: 269.9.1/857 - Release Date: 6/20/2007 = 2:18 PM =20 From PMonfils <@t> Lifespan.org Wed Jun 20 17:13:15 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Jun 20 17:13:23 2007 Subject: [Histonet] picro sirius red In-Reply-To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E273CA3@LSRIEXCH1.lsmaster.lifespan.org> I find I get cleaner differentiation by using 1 ml HCl per liter of distilled water instead of the acetic acid solution. 2 minutes differentiation usually works well, but it can be used up to 5 minutes if necessary. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Liz Chlipala > Sent: Wednesday, June 20, 2007 5:42 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] picro sirius red > > Hello everyone > > We are running some picro sirius red for image analysis and were running into a bit of a problem. We are staining rat heart, the collagen staining is quite nice but some of the muscle is staining a tad bit orangish rather than a really nice yellow. Which is not a problem when you look under the microscope but once the images are captured for analysis thats were we run into problems. We have tried making up fresh staining solution, etc. differenciating more in the acetic acid but the muscle is stil a bit orange in color. Any help would be appreciated. > > Thanks in advance > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Manager > Premier Laboratory, LLC > P.O. Box 18592 > Boulder, CO 80308 > phone (303) 735-5001 > fax (303) 735-3540 > HYPERLINK "mailto:liz@premierlab.com"liz@premierlab.com > HYPERLINK "http://www.premierlab.com/"www.premierlab.com > > Ship to Address: > > Premier Laboratory, LLC > University of Colorado at Boulder > MCDB, Room A3B40 > Boulder, CO 80309 > > > No virus found in this outgoing message. > Checked by AVG Free Edition. > Version: 7.5.472 / Virus Database: 269.9.1/857 - Release Date: 6/20/2007 2:18 PM > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jnocito <@t> satx.rr.com Wed Jun 20 17:16:15 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Jun 20 17:16:19 2007 Subject: [Histonet] BONE MARROW DECAL References: <20070620155321.72871.qmail@web51601.mail.re2.yahoo.com> Message-ID: <00e501c7b388$9e8774b0$d49eae18@yourxhtr8hvc4p> Biocare Medical (800) 799-9499 has I.E.D. (ionic exchange decal). It's little beads in solution. You can decal 20 bone marrow biopsies before tossing the solution out. The hempath I used to work for was ecstatic because it didn't interfere with immunos. We place the blocks in the solution for one hour, rinse and process. We also fix our bone marrows in B-Plus and the two solutions are compatible. Joe, out of work for now, bum ----- Original Message ----- From: "MaryAnn Hancock" To: Sent: Wednesday, June 20, 2007 10:53 AM Subject: [Histonet] BONE MARROW DECAL >I am interested to know what Decal fluid is being used for Bone Marrow Core >Biopsies and how long you fix them for. > > Thanks > > MaryAnn > > > --------------------------------- > Sick sense of humor? Visit Yahoo! TV's Comedy with an Edge to see what's > on, when. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Wed Jun 20 18:17:28 2007 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Jun 20 18:17:38 2007 Subject: [Histonet] BONE MARROW DECAL Message-ID: This is our protocol: Solutions: 1. Fixative (Formal Alcohol) 40% Formaldehyde 200ml 95% Ethanol 1800ml Add 3 drops of 1% eosin to colour the solution Warning Toxic - see MSDS 2. Formic Acid/Formalin Decalcification Solution Pathtech Cat No ADF.J (4% Formaldehyde, 33% Formic acid, 0.85% Sodium Chloride) Procedure: 1. Allow trephine to fix in formal alcohol for 3? to 4 hours (best overnight) 2. Rinse the trephine in water and place in Decalcifying solution for 3-4 hours. 3. Place trephinein biopsy cassette. 4. Place in 10% formalin to await processing. 5. If calcium is present when cutting, then surface decal in RDO for 20mion, wash and recut References 1. Blake, J (1970) "Histological Methods of the University of Melbourne" p15. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MaryAnn Hancock Sent: Thursday, 21 June 2007 1:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] BONE MARROW DECAL I am interested to know what Decal fluid is being used for Bone Marrow Core Biopsies and how long you fix them for. Thanks MaryAnn --------------------------------- Sick sense of humor? Visit Yahoo! TV's Comedy with an Edge to see what's on, when. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From koellingr <@t> comcast.net Wed Jun 20 20:47:28 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Wed Jun 20 20:47:35 2007 Subject: [Histonet] non-mouse anti-flag antibody Message-ID: <062120070147.7794.4679D8B00006D26600001E7222070032019D09020704040A0105@comcast.net> Caroline, SigmaAldrich has a large variety of anti-FLAG antibodies. Have the usual mouse culprits but if that is not what you want, I've used their rabbit monoclonal anti-FLAG Ab successfully on mouse tissue (didn't try brain). They also have rabbit polyclonal anti-FLAG but did not try. I'm not a big mouse-on-mouse fan at all. But I have used their directly conjugated mouse monoclonal anti-FLAGs on mouse tissue (again, not brain). Remember, if you don't know, that anti-FLAG antibodies are a bit idiosyncratic. Some MUST have calcium (Ca2+) ions present during incubation or you will get no binding. Some are not like that but many are. Ray Koelling Phenopath Labs Seattle, WA -------------- Original message -------------- From: cbass@bidmc.harvard.edu > Hello, > > I was hoping someone could suggest a good anti-flag antibody for use in mouse > brain. A lot of anti-flags are monoclonal, which is not very good for mouse > tissue obviously. Does anyone have a suggestion? The amino acid sequence of > the flag is DYKDDDDK. A FITC tag would be helpful as well, but right now just > having a non-mouse antibody would be great. Also, has anyone tried the > mouse-on-mouse kits in the brain or have suggestions for going this route? > > Thanks! > > Caroline > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From koellingr <@t> comcast.net Wed Jun 20 21:18:52 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Wed Jun 20 21:18:58 2007 Subject: [Histonet] microglia in mouse brains Message-ID: <062120070218.1100.4679E00B0008B4860000044C22058863609D09020704040A0105@comcast.net> Yes, you can use F4/80 on microglia in brain if you aware of potential problems regarding the activation state of the microglia. Also try BM8 and on most data sheets they will explain the much mis-understood difference between F4/80 and BM8. Using those 2 antibodies can give you very similar but very subtle and observable differences in the stained population of cells whether in brain or spleen in tissue IHC or in flow. Ray Koelling Phenopath Labs Seattle, WA -------------- Original message -------------- From: "Liz Chlipala" > Brett > > I'm not positive but I think that you might be able to use F4/80 as microglia > marker in mouse brain. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Manager > Premier Laboratory, LLC > P.O. Box 18592 > Boulder, CO 80308 > phone (303) 735-5001 > fax (303) 735-3540 > liz@premierlab.com > www.premierlab.com > > Ship to Address: > > Premier Laboratory, LLC > University of Colorado at Boulder > MCDB, Room A3B40 > Boulder, CO 80309 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett > M > Sent: Wednesday, June 20, 2007 9:46 AM > To: histonet@lists.utsouthwestern.edu; ihcrg@googlegroups.com > Subject: [Histonet] microglia in mouse brains > > A colleague is looking for an antibody & protocol for staining microglia in > mice. > > Any suggestions? > > Thx, > Brett > > Brett M. Connolly, Ph.D. > Research Fellow > MRL, Imaging Research > Merck & Co., Inc. > WP-44K > PO Box 4 > West Point, PA 19486 > PH 215-652-2501 > fax. 215-993-6803 > e-mail. brett_connolly@merck.com > > > ------------------------------------------------------------------------------ > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New > Jersey, USA 08889), and/or its affiliates (which may be known outside the United > States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - > direct contact information for affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, > proprietary copyrighted and/or legally privileged. It is intended solely for the > use of the individual or entity named on this message. If you are not the > intended recipient, and have received this message in error, please notify us > immediately by reply e-mail and then delete it from your system. > > ------------------------------------------------------------------------------ > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.5.472 / Virus Database: 269.9.1/857 - Release Date: 6/20/2007 2:18 PM > > > No virus found in this outgoing message. > Checked by AVG Free Edition. > Version: 7.5.472 / Virus Database: 269.9.1/857 - Release Date: 6/20/2007 2:18 PM > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cabramley <@t> students.latrobe.edu.au Wed Jun 20 21:37:07 2007 From: cabramley <@t> students.latrobe.edu.au (CLAIRE ANN KENTLER) Date: Wed Jun 20 21:37:19 2007 Subject: [Histonet] CD4 and CD8 Staining Message-ID: <91DC98B386D8614487170C699BE9299F04FE5B4B@stexchange1.students.ltu.edu.au> Hi all, I'm relatively new to all this, but I am looking for an immunohistochemical stain for CD4 and CD8 in calf tissue. I was hoping to find a protocol for formalin-fixed paraffin embedded sections, as bovine WC1 can be isolated in these sections and I am looking at cell proliferation and T and B lymphocyte percentages in this manner. All the literature I've come across indicates the need for either a modified fixative, or frozen sections. (Thus requiring double the sample collection, or changing of previous protocols and study repetition). Has anyone come across a good retrieval method for CD4 and CD8 in ffpe sections that we could try in bovine tissue, or are frozen sections the way to go? Thanks, Claire ************************************************************************ *************************************** Claire Kentler Bachelor of Animal Science (Hons) Postgraduate Student Department of Agricultural Sciences La Trobe University Phone: 9479 1048 E-mail: cabramley@students.latrobe.edu.au From koellingr <@t> comcast.net Wed Jun 20 22:06:21 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Wed Jun 20 22:06:24 2007 Subject: [Histonet] NK cells in mouse frozens(long technical reply) Message-ID: <062120070306.24742.4679EB2C000DD9E2000060A622058863609D09020704040A0105@comcast.net> If not interested in technical data - please delete. Sonya, I've done NK cells in mouse tissue that were frozen. But I've also used the zinc, formalin free "fixative" of Beckstead that BD sells but I'm not sure what most people call it. It is zinc and calcium ions in a Tris buffer that can be used with paraffin sections. It works for IHC. But lets just talk frozen mouse tissue. Looked in lymphoid tissues; spleen, lymph nodes, Peyers patches, etc with the warning that will follow at the end. Have used Ab's to NK1.1, CD49b, alpha 2 integrin and NKG2D. Just remember that as far as I know, there is no single target that defines NK cells and no other cells. CD3+NK1.1- equals T cells, CD3+NK1.1+ equals NKT cells and CD3-NK1.1+ equals NK cells. NKG2D exists on many NK cells but on minor alpha/beta T-cells. DX5 is a great antibody to the CD49b,alpha 2 integrin complex and while it is not haplotype restricted and works on most strains, some other NK markers are haplotype restricted. So not like staining vimentin or CD4. The short of it is that you can stain with these and other "NK" cell antibodies in murine frozen lymphoid tissue. BUT, remember, there might not be a lot of them around to see in a 6 micron section. To collect and identify NK cells by flow cytometry you cannot put on a single marker and there they are. They are a very, very, very small population electronically gated by a stain out of a very, very small population electronically gated by a different staining marker out of a small population of the whole. Pretty easy to do with a cytometer looking at 25,000 gated events in a matter of seconds but iffy if on that one section you are looking at. Could give yourself a better chance by creating better control tissue. There are many classical in-vivo ways to increase NK cells (IL-2, IL-15) administration, and many others. If you could get someone to get you a mouse that has been manipulated, NK cells might be easier to find by flow and certainly I've found it easier to be confiden t I've found them in 1 or 2 stained sections I was looking at. Ray Koelling Phenopath Labs Seattle, WA -------------- Original message -------------- From: "Martin S." > Hi All, > > In addition to my other question today (about labelling with antiserum) > - has anyone successfully labelled NK cells in frozen sections of mouse > tissues? If so which tissue and which antibodies did you use? > > Thanks > Sonya > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaimie_hk <@t> yahoo.co.uk Thu Jun 21 05:31:58 2007 From: jaimie_hk <@t> yahoo.co.uk (Jaimie Hoh) Date: Thu Jun 21 05:32:04 2007 Subject: [Histonet] frozen sections on monkey eye In-Reply-To: Message-ID: <557793.33839.qm@web25914.mail.ukl.yahoo.com> Hello Jennifer, I've worked in an eye institute before and i have done a lot of frozen mouse and rat eyes. Normally i'll have a lot of difficulties in cutting the lens as when it is fixed in 4% PFA, it hardens. I've read in one of the previous email from histonet that Perenyi's fluid is a good fixative for eye as both Nitric and chromic acids have a softening effect on the tissuesand ethanol is a coagulant fixative. It is good as it fixes retina really well with little separation of the layers and does not harden the lens. I hope this will help. Jaimie --- "Harvey, Jennifer Lynn" wrote: > Histonet: > > > > I thought years of bone work I had done some of the > most challenging > histology. We move to Nashville and I took a job in > eye research. Was I > crazy??? This is worse. I need to cut frozen > sections on 4%PFA fixed > monkey eyes? Any ideas?? > > > > > > Thanks!!!! > > > > > > Jennifer Harvey > > Vanderbilt Eye Institute > > RM 8105 MCE North Tower > > 1215 21st Ave. South > > Nashville, TN 37232-8808 > > Phone: 615-936-1486 > > > > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___________________________________________________________ Yahoo! Mail is the world's favourite email. Don't settle for less, sign up for your free account today http://uk.rd.yahoo.com/evt=44106/*http://uk.docs.yahoo.com/mail/winter07.html From Terry.Marshall <@t> rothgen.nhs.uk Thu Jun 21 06:12:15 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Thu Jun 21 06:12:00 2007 Subject: [Histonet] Small cell carcinoma Message-ID: <5C0BED61F529364E86309CADEA63FEF25E6C1B@TRFT-EX01.xRothGen.nhs.uk> Ah, an H&E. What a good idea. A good H&E - even better idea. In what possible circumstance other than severe crushing artefact, would you possibly need immunochemistry for a diagnosis of small cell carcinoma? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: 20 June 2007 21:12 To: Lynette Pavelich; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Small cell carcinoma A good H&E and, if necessary, CK8/18 (delicate, sometimes punctate cytoplasmic reactivity) and chromogranin (rare, granular cytoplasmic staining). Synaptophysin is not one of my favorite antibodies due to specificity issues. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Lynette Pavelich" 06/20/07 2:31 PM >>> Hello, I am curious to see what other institutions are using to diagnose small cell carcinoma in human formalin fixed, paraffin tissue. I have run into some literature that states that synaptophysin will stain positive 90% of the time, and in other articles will stain positive only 50% of the time. Are there other antibodies that are more specific that you have found work better?? Awaiting your knowledge and experience. Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810-257-9948 fax: 810-762-7082 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pmayo <@t> cscc.edu Thu Jun 21 06:56:57 2007 From: pmayo <@t> cscc.edu (Peggy Mayo) Date: Thu Jun 21 06:57:20 2007 Subject: [Histonet] training for non-certified HTs Message-ID: <467A2F49020000670000E8A4@gwgate.cscc.edu> To all Supervisors and Managers, If you are interested in a formal training program to get your non-certified HTs eligible for certification, please visit my program website at: www.cscc.edu/histology. This is a 3 quarter (approximately 9 months) distance program with an integrated clinical experience. This experience can be fulfilled in approved laboratories (that meet our accreditation standards) and after a clinical affiliate agreement has been signed. The next class will begin Fall, 2008, with applications due May 1, 2008. If you are a supervisor/manager and are interested, you may contact me directly at this email or my phone # below. Thank you. Peggy Mayo Peggy Mayo M.Ed. MLT (ASCP) Multi-Competency Health Technology 614-287-2608 or 800-621-6407 ext. 2608 Fax 614-287-3854 From Lynne.Bell <@t> hitchcock.org Thu Jun 21 07:32:16 2007 From: Lynne.Bell <@t> hitchcock.org (Bell, Lynne) Date: Thu Jun 21 07:32:25 2007 Subject: [Histonet] Countertop Color In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B8F4507@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: We recently moved into our new lab and we have black countertops. The one thing that I have noticed in paraffin REALLY is a glaring contrast and makes the lab look slightly messy at all times........ I had wanted a lighter color (a grey), but black was less expensive so we had to go with that color. Lynne From KARENADAMS <@t> comcast.net Thu Jun 21 07:49:42 2007 From: KARENADAMS <@t> comcast.net (KARENADAMS@comcast.net) Date: Thu Jun 21 07:49:48 2007 Subject: [Histonet] Re: Leica XL staining racks Message-ID: <062120071249.25826.467A73E60007E74C000064E22207021053ACB3BEBBBEB2BAADBEB5@comcast.net> does anyone happen to have a good source (ie NOT 238.00/rack) for the Leica XL staining racks....I just think this is ridiculous! Thanks in advance :) Karen -- Karen Adams Pathology Laboratories West 9303 Park West Blvd Knoxville, TN 37923 (865) 690-2111 FAX (865) 691-1623 From relia1 <@t> earthlink.net Thu Jun 21 07:53:20 2007 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Jun 21 07:53:27 2007 Subject: [Histonet] RELIA Histology Job Alert!!! Histology Manager in Chicago, IL Message-ID: Hello Histonetters, I have an exciting opportunity for an experienced Histology Manager in a hospital environment located in Chicago, IL. The compensation package is excellent, the work is challenging and the sky is the limit. The position is of course full time and day shift Monday - Friday. The requirements of this position are a BS degree with emphasis in the sciences/health care field. HT (ASCP) certification required, HTL (ASCP) desired. Broad knowledge of anatomic pathology usually acquired through at least 5 years of experience, preferably within an academic institution. If you would like more information or know of someone else who might be interested, please contact me at relia1@earthlink.net or 866-607-3542. I am available to discuss the opportunity at your convenience including after hours. Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net From ccpath <@t> gmail.com Thu Jun 21 08:08:26 2007 From: ccpath <@t> gmail.com (j k) Date: Thu Jun 21 08:08:32 2007 Subject: [Histonet] RELIA Histology Job Alert!!! Histology Manager in Chicago, IL In-Reply-To: References: Message-ID: Need locum for vacation help; beautiful coastal Carolina. Looking for cytotechnologist for 1-2 weeks stints this summer. 35-45 paps/day; 1-5 non gyns/day. Call Jim 910.540.1268 From jstaruk <@t> masshistology.com Thu Jun 21 08:18:17 2007 From: jstaruk <@t> masshistology.com (Jim Staruk) Date: Thu Jun 21 08:17:45 2007 Subject: [Histonet] Re: Leica XL staining racks In-Reply-To: <062120071249.25826.467A73E60007E74C000064E22207021053ACB3BEBBBEB2BAADBEB5@comcast.net> References: <062120071249.25826.467A73E60007E74C000064E22207021053ACB3BEBBBEB2BAADBEB5@comcast.net> Message-ID: <000001c7b406$a2772830$4001a8c0@FrontOffice> Can you describe these? I have a Carl Zeiss DS-50 stainer with lots of extra vessels and metal slide carriers that hold 50 slides each. Might these fit? Jim ______________________ Jim Staruk Mass Histology Service www.masshistology.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of KARENADAMS@comcast.net Sent: Thursday, June 21, 2007 8:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Leica XL staining racks does anyone happen to have a good source (ie NOT 238.00/rack) for the Leica XL staining racks....I just think this is ridiculous! Thanks in advance :) Karen -- Karen Adams Pathology Laboratories West 9303 Park West Blvd Knoxville, TN 37923 (865) 690-2111 FAX (865) 691-1623 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly <@t> merck.com Thu Jun 21 08:24:59 2007 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Thu Jun 21 08:25:38 2007 Subject: [Histonet] Thanks- microglia & frozen to paraffin In-Reply-To: <062120070218.1100.4679E00B0008B4860000044C22058863609D09020704040A0105@comcast.net> References: <062120070218.1100.4679E00B0008B4860000044C22058863609D09020704040A0105@comcast.net> Message-ID: <63EA0607835FBA4689CEA9EA8B4826923E98F7@usctmx1141.merck.com> Thanks very much for all of your responses to my questions Brett Brett M. Connolly, Ph.D. Research Fellow MRL, Imaging Research Merck & Co., Inc. WP-44K PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com ------------------------------------------------------------------------------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------------ From mari.ann.mailhiot <@t> leica-microsystems.com Thu Jun 21 09:04:15 2007 From: mari.ann.mailhiot <@t> leica-microsystems.com (mari.ann.mailhiot@leica-microsystems.com) Date: Thu Jun 21 09:04:26 2007 Subject: [Histonet] Re: Leica XL staining racks In-Reply-To: <062120071249.25826.467A73E60007E74C000064E22207021053ACB3BEBBBEB2BAADBEB5@comcast.net> Message-ID: Hi Karen You can order part number 14047533643 a pack of 5 plastice racks for 185.00, or one metal rack part number 14045633919 for 185.00. You can order directly from Leica. Just fax your order to 847 317 7268. Regards Mari Ann Mailhiot BA HT ASCP Application Specialist Leica Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com KARENADAMS@comcas t.net Sent by: To histonet-bounces@ histonet@lists.utsouthwestern.edu lists.utsouthwest cc ern.edu Subject [Histonet] Re: Leica XL staining 06/21/2007 07:49 racks AM does anyone happen to have a good source (ie NOT 238.00/rack) for the Leica XL staining racks....I just think this is ridiculous! Thanks in advance :) Karen -- Karen Adams Pathology Laboratories West 9303 Park West Blvd Knoxville, TN 37923 (865) 690-2111 FAX (865) 691-1623 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From Dorothy.L.Webb <@t> HealthPartners.Com Thu Jun 21 09:24:14 2007 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Thu Jun 21 09:24:57 2007 Subject: [Histonet] cores Message-ID: <0E394B648E5284478A6CCB78E5AFDA2703640DAD@hpes1.HealthPartners.int> We decalcify our bone marrow cores in "Rapid Cal Immuno" for 2 hours. The product is from BBC Biochemical. As the name implies, it is IHC compatible! Also, we use CoPath for our AP computer system and love it. It is user friendly and compatible with many other systems and vendors! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From emerald_lake77 <@t> yahoo.com Thu Jun 21 09:30:32 2007 From: emerald_lake77 <@t> yahoo.com (GT Hebert) Date: Thu Jun 21 09:30:35 2007 Subject: [Histonet] Collagen IV staining pattern in mouse kidney?? Message-ID: <557436.78516.qm@web31714.mail.mud.yahoo.com> Hello, I am using the Chemicon Polyclonal Rabbit anti-mouse Collagen IV on mouse kidney samples that have been fixed in 4% paraformaldehyde, processed rountinely and embedded in paraffin. I cut the sections at 3 microns and ran a routine IHC protocol using Proteinase K (DAKO, ready-to-use, 10mins) as a retreival method and DAKO Envision (HRP) kit as secondary. Is anyone familar with Collagen IV staining pattern within the kidney?? At the moment for all my samples I am receiving areas of weak staining in the tubules closest to the outside edge of the kidney. Otherwise, the remaining basement membranes and areas within the glomeruli are stained very nicely (dark DAB staining). The faint staining appears in patches and I cannot tell if it is artifact (over digestion with Prot. K), a technical error or an actually pathological phenomena. I ran two batches, one baked the day of staining at 65 degree C and another overnight at 37 degrees C thinking that maybe baking at high temps damaged the epitope. But the pattern I received is the same for both batches. Any information would be helpful. I am just not familar with the "normal" pattern that collegen IV stains within the kidney. Thank you very much. Sincerely, Gustave Hebert Scientist II Wyeth Research Cambridge MA --------------------------------- 8:00? 8:25? 8:40? Find a flick in no time with theYahoo! Search movie showtime shortcut. From asmith <@t> mail.barry.edu Thu Jun 21 09:39:25 2007 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Thu Jun 21 09:39:30 2007 Subject: [Histonet] Countertop Color In-Reply-To: Message-ID: <7DBCCC1FBC77C94F99F920D0CA6400B61E40B2@exchsrv01.barrynet.barry.edu> A black countertop (or a sheet of black paper) makes it easier to detect bubbles under a freshly-mounted coverslip. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bell, Lynne Sent: Thursday, June 21, 2007 8:32 AM To: Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Countertop Color We recently moved into our new lab and we have black countertops. The one thing that I have noticed in paraffin REALLY is a glaring contrast and makes the lab look slightly messy at all times........ I had wanted a lighter color (a grey), but black was less expensive so we had to go with that color. Lynne _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From jessgrocki <@t> yahoo.com Thu Jun 21 10:21:34 2007 From: jessgrocki <@t> yahoo.com (Jessica Piche) Date: Thu Jun 21 10:21:40 2007 Subject: [Histonet] Analyte Specific Reagent Message-ID: <331353.39055.qm@web82010.mail.mud.yahoo.com> Hi All, How do I know if an antibody is a Analyte Specific Reagent ? Can I find that on the antibody insert? Thanks, Jessica Piche-Grocki, HT From jessgrocki <@t> yahoo.com Thu Jun 21 10:22:36 2007 From: jessgrocki <@t> yahoo.com (Jessica Piche) Date: Thu Jun 21 10:22:40 2007 Subject: [Histonet] Fwd: Analyte Specific Reagent Message-ID: <214425.40887.qm@web82010.mail.mud.yahoo.com> Note: forwarded message attached. From SDrew <@t> uwhealth.org Thu Jun 21 10:25:52 2007 From: SDrew <@t> uwhealth.org (Drew Sally A.) Date: Thu Jun 21 10:25:56 2007 Subject: [Histonet] Analyte Specific Reagent In-Reply-To: <331353.39055.qm@web82010.mail.mud.yahoo.com> Message-ID: Yes, if the company is thorough, you can usually find it somewhere on the data sheet. If you can't, definitely e-mail the company and get it in writing for your file. Sally Ann Drew, MT(ASCP) IHC/ISH Laboratory University of Wisconsin Hosp. & Clinics Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica Piche Sent: Thursday, June 21, 2007 10:22 AM To: histonet Subject: [Histonet] Analyte Specific Reagent Hi All, How do I know if an antibody is a Analyte Specific Reagent ? Can I find that on the antibody insert? Thanks, Jessica Piche-Grocki, HT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jennifer.harvey <@t> Vanderbilt.Edu Thu Jun 21 10:46:34 2007 From: jennifer.harvey <@t> Vanderbilt.Edu (Harvey, Jennifer Lynn) Date: Thu Jun 21 10:46:40 2007 Subject: [Histonet] what do people for ISH Message-ID: I am working with someone that want to know what people are doing about RNases from the start of killing the animal to cutting the slides and any solutions/ equipment along the way. What is realalistic? They have been doing ISH for years and now have someone in the lab that is asking for everything to be RNase free. Can that even be done? Thanks Jennifer Harvey Vanderbilt Eye Institute RM 8105 MCE North Tower 1215 21st Ave. South Nashville, TN 37232-8808 Phone: 615-936-1486 From godsgalnow <@t> aol.com Thu Jun 21 10:57:40 2007 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Thu Jun 21 10:57:58 2007 Subject: [Histonet] ProVysion Message-ID: <8C9823407DC0A9B-D98-15757@FWM-R18.sysops.aol.com> Is anyone out there doing the ProVysion Multi-color Probe Kit on prostate tissue?? I would be very interested to hear from anyone.... Thank you, Roxanne Soto HT(ASCP)QIHC ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From pmcardle <@t> ebsciences.com Thu Jun 21 11:11:48 2007 From: pmcardle <@t> ebsciences.com (Phil McArdle) Date: Thu Jun 21 11:11:55 2007 Subject: [Histonet] Counter/Clothes Color In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B8F450B@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B8F450B@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <467AA344.5050503@ebsciences.com> How about "paisley?" I'm pretty sure you'll be able to find paisley scrubs, though I doubt a paisley counter exists anywhere... As an ex work-at-home-dad who had to take my then-infant son to clients, I got to LOVE paisley, especially ties: that pattern can hide a multitude of sins. Paisley was so adept at camouflaging spit-up and sippy-cup-collateral-damage, it ought to take a little H&E and paraffin in stride. :-) -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Mobile: 860.597.6796 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com I skate to where the puck is going to be, not to where it's been. - Wayne Gretsky You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it, and it was probably my mistake anyway. So please do the right thing and make this e-mail go away. Thank you. Breeden, Sara wrote: > Thanks everybody for the ideas. I think the > solid-surface-hematoxylin-paraffin-carbol-fuchsin-tissue-biopsy-colored > countertops I've picked out will work just fine. Now if I could only > decide what color clothes to wear to work that perform the same > camouflage... I'll be lucky to even see this lab before I retire in > 3.5 years! It's been in the works for 3 years so far. I'll tell you, > though, I sure am glad I took that seminar at NSH a couple years ago > about planning a new lab space - that's been a PRICELESS resource when > working with architects, not to mention the input from Histonet. > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Mobile: 860.597.6796 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com I skate to where the puck is going to be, not to where it's been. - Wayne Gretsky You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it, and it was probably my mistake anyway. So please do the right thing and make this e-mail go away. Thank you. From ian.montgomery <@t> bio.gla.ac.uk Thu Jun 21 11:28:28 2007 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Thu Jun 21 11:28:36 2007 Subject: FW: [Histonet] Counter/Clothes Color Message-ID: <005701c7b421$331122f0$6424d182@IBLS.GLA.AC.UK> Phil, As a Paisley buddy you've got me upset and just before I go to Paisley. Please, use upper case P, the pattern refers to one that was designed and originally manufactured in Paisley. Paisley is a small town in the west of Scotland, once famous for cotton and weaving. The weavers made silk and cotton shawls enriched with the Paisley pattern. It's also famous for poets and beautiful women. Two miles west is the village of Elderslie, birth place of Scotland's most famous son, William Wallace. Thursday rant over, I'm away home. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, G12 8QQ. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Phil McArdle Sent: 21 June 2007 17:12 To: Breeden, Sara Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Counter/Clothes Color How about "paisley?" I'm pretty sure you'll be able to find paisley scrubs, though I doubt a paisley counter exists anywhere... As an ex work-at-home-dad who had to take my then-infant son to clients, I got to LOVE paisley, especially ties: that pattern can hide a multitude of sins. Paisley was so adept at camouflaging spit-up and sippy-cup-collateral-damage, it ought to take a little H&E and paraffin in stride. :-) -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Mobile: 860.597.6796 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com I skate to where the puck is going to be, not to where it's been. - Wayne Gretsky You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it, and it was probably my mistake anyway. So please do the right thing and make this e-mail go away. Thank you. Breeden, Sara wrote: > Thanks everybody for the ideas. I think the > solid-surface-hematoxylin-paraffin-carbol-fuchsin-tissue-biopsy-colored > countertops I've picked out will work just fine. Now if I could only > decide what color clothes to wear to work that perform the same > camouflage... I'll be lucky to even see this lab before I retire in > 3.5 years! It's been in the works for 3 years so far. I'll tell you, > though, I sure am glad I took that seminar at NSH a couple years ago > about planning a new lab space - that's been a PRICELESS resource when > working with architects, not to mention the input from Histonet. > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Mobile: 860.597.6796 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com I skate to where the puck is going to be, not to where it's been. - Wayne Gretsky You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it, and it was probably my mistake anyway. So please do the right thing and make this e-mail go away. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tim.morken <@t> thermofisher.com Thu Jun 21 11:32:37 2007 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Thu Jun 21 11:32:56 2007 Subject: [Histonet] Analyte Specific Reagent In-Reply-To: <331353.39055.qm@web82010.mail.mud.yahoo.com> References: <331353.39055.qm@web82010.mail.mud.yahoo.com> Message-ID: <6BFF6D137DF6BC43B33891BA96E83B1982B5C8@PGHCR-EXMB-VS-1.na.fshrnet.com> Jessica, If it is an analyate specific reagent it should say so right at the top, or under "intended use." The ASR datasheet will be devoid of any testing parameters (by law, the lab buying an ASR is required to validate it and the vendor is not allowed to provide test parameter information). Tim Morken Technical Support Manager Anatomical Pathology ThermoFisher Scientific / Lab Vision Products -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica Piche Sent: Thursday, June 21, 2007 8:22 AM To: histonet Subject: [Histonet] Analyte Specific Reagent Hi All, How do I know if an antibody is a Analyte Specific Reagent ? Can I find that on the antibody insert? Thanks, Jessica Piche-Grocki, HT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> aol.com Thu Jun 21 12:54:03 2007 From: rsrichmond <@t> aol.com (rsrichmond@aol.com) Date: Thu Jun 21 12:54:17 2007 Subject: [Histonet] Re: countertop colors and patterns In-Reply-To: <200706211300.1ee467aae9c17b@rly-yd01.mx.aol.com> References: <200706211300.1ee467aae9c17b@rly-yd01.mx.aol.com> Message-ID: <8C982444A1B9166-3F8-81FE@WEBMAIL-DC12.sysops.aol.com> Ian Mongtgomery notes: Phil [McArdle}, As a Paisley buddy you've got me upset and just before I go to Paisley. Please, use upper case P, the pattern refers to one that was designed and originally manufactured in Paisley. Paisley is a small town in the west of Scotland, once famous for cotton and weaving. The weavers made silk and cotton shawls enriched with the Paisley pattern. It's also famous for poets and beautiful women. Two miles west is the village of Elderslie, birth place of Scotland's most famous son, William Wallace. Thursday rant over, I'm away home. Ian. Paisley designs were certainly perfected and long manufactured in the Scots town of Paisley, in the mid 19th century. The basic "Paramecium paisleyi" motif is however of southern Asian origin, and antedates the 19th century. It seems to have originally depicted a drooping plant or tree. Transitional forms between the drooping plant and the fully developed Paramecium paisleyi are still to be seen in tribal rugs and fabrics - I've assembled a collection of photos of them in the last few years. The fully developed Paramecium paisleyi reminds me of those things that appear in my eyes right before a migraine attack, or perhaps to one of those pharmacologic states of mind some of us listened to Sergeant Pepper's Lonely Hearts Club Band to, back when that was new... The woods of Arcady are dead, and over is their antique joy... (Yeats) Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From carl.hobbs <@t> kcl.ac.uk Thu Jun 21 13:12:09 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Thu Jun 21 13:12:26 2007 Subject: [Histonet] RE: microglia in mouse brains Message-ID: <000501c7b42f$af9f1710$4001a8c0@carlba65530bda> I understand that the Antigen is designated as F4/80 and BM8 is but a clone designation of a monoclonal against a part of the F4/80 Ag? I tried BM8 on pwax rat/mouse brains and it was negative. Perhaps it does not work in that particular application or, according to AbDSerotec, clone BM8 is reported not to identify microglia, unlike the "original " Ab against F4/80, clone C1:A3-1. I have not yet tried the latter. However, any comments regarding microglial staining in mouse/rat brains( p.wax sections, after HIER) of Wako's Iba1 Ab 01-19741, would be appreciated. Please see here http://www.immunoportal.com/index.php for pics. Navigate to Image gallery and search for "microglia" and then "Wako", for eg. Also, I noted that an anti p-tyrosine Ab ( p-tyr) seems to be highly selective for microglia, in the same application; ( please see same site/Image gallery for pics, also. Any expert comments on these pics would be most welcome. If there are any, perhaps you would be kind enough to post your comments in that forum, also? I am a "jack-of-all trades" Histologist/Immunohistologist , rather than an expert in any particular field. Carl From drbugge <@t> gmail.com Thu Jun 21 13:17:09 2007 From: drbugge <@t> gmail.com (Dawn Bugge) Date: Thu Jun 21 13:17:13 2007 Subject: [Histonet] Microwave Processing Message-ID: <1c4db3750706211117r3b9fa16djede227bbca314fb6@mail.gmail.com> I was wondering if anyone had some pros and cons for microwave processing. We use the VIP processor now but are looking into microwave processing. Haven't found much information on the internet about what people really think about these products. Thanks for any information you have. -- Dawn R Bugge Seattle Histology Laboratory From rjbuesa <@t> yahoo.com Thu Jun 21 14:04:06 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jun 21 14:04:11 2007 Subject: [Histonet] Microwave Processing In-Reply-To: <1c4db3750706211117r3b9fa16djede227bbca314fb6@mail.gmail.com> Message-ID: <495198.43864.qm@web61218.mail.yahoo.com> Dawn: Under separate cover I am sending you an article I wrote with dealing with the overall effect of MW processing in the HL workflow. Ren? J. Dawn Bugge wrote: I was wondering if anyone had some pros and cons for microwave processing. We use the VIP processor now but are looking into microwave processing. Haven't found much information on the internet about what people really think about these products. Thanks for any information you have. -- Dawn R Bugge Seattle Histology Laboratory _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Boardwalk for $500? In 2007? Ha! Play Monopoly Here and Now (it's updated for today's economy) at Yahoo! Games. From gcallis <@t> montana.edu Thu Jun 21 14:32:01 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Jun 21 14:32:12 2007 Subject: [Histonet] frozen sections on monkey eye In-Reply-To: <557793.33839.qm@web25914.mail.ukl.yahoo.com> References: <557793.33839.qm@web25914.mail.ukl.yahoo.com> Message-ID: <6.0.0.22.1.20070621132223.01b35f60@gemini.msu.montana.edu> Ethanol is antifreeze and if you have that in the tissue, you will probably have snap freezing issues that further sectioning problems. This would be eliminated by the sucrose cryoprotection before snap freezing however. You can try raising the cutting temperature so it is higher say at -16C to keep the tissues from being crunchy and harder. If you plan to do immunohistochemistry, you should be aware that Perenyi's fluid is also used for decalcifying bone and it may compromise your tissue antigens when exposed to the nitric acid. Another possibility is to cut the eyes with a tungsten carbide knife, much as one would do for undecalcified bone sections, and they do make c profile tungsten carbide knives at Delaware Diamond Knives. They may even have a demo available to try out, before you spend the big bucks for your own knife. These are designed to cut through some extremely tough tissues even decalcified bone frozens at times might be easier with a TC knife. Another possibility is to use the Cryojane tape transfer system to maintain all parts of the eye. At 04:31 AM 6/21/2007, you wrote: >Hello Jennifer, > >I've worked in an eye institute before and i have done >a lot of frozen mouse and rat eyes. Normally i'll have >a lot of difficulties in cutting the lens as when it >is fixed in 4% PFA, it hardens. >I've read in one of the previous email from histonet >that Perenyi's fluid is a good fixative for eye as >both Nitric and chromic acids have a softening effect >on the tissuesand ethanol is a coagulant fixative. It >is good as it fixes retina really well with little >separation of the layers and does not harden the lens. > >I hope this will help. > >Jaimie > > >--- "Harvey, Jennifer Lynn" > wrote: > > > > I thought years of bone work I had done some of the > > most challenging > > histology. We move to Nashville and I took a job in > > eye research. Was I > > crazy??? This is worse. I need to cut frozen > > sections on 4%PFA fixed > > monkey eyes? Any ideas?? Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From jnocito <@t> satx.rr.com Thu Jun 21 15:33:10 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Jun 21 15:33:19 2007 Subject: [Histonet] Re: countertop colors and patterns References: <200706211300.1ee467aae9c17b@rly-yd01.mx.aol.com> <8C982444A1B9166-3F8-81FE@WEBMAIL-DC12.sysops.aol.com> Message-ID: <008a01c7b443$62cc8e90$d49eae18@yourxhtr8hvc4p> what ever happened to basic black? Joe, no longer the toe. ----- Original Message ----- From: To: Sent: Thursday, June 21, 2007 12:54 PM Subject: [Histonet] Re: countertop colors and patterns > > Ian Mongtgomery notes: > Phil [McArdle}, > As a Paisley buddy you've got me upset and just before I go to > Paisley. Please, use upper case P, the pattern refers to one that was > designed and originally manufactured in Paisley. Paisley is a small town > in > the west of Scotland, once famous for cotton and weaving. The weavers made > silk and cotton shawls enriched with the Paisley pattern. It's also famous > for poets and beautiful women. Two miles west is the village of Elderslie, > birth place of Scotland's most famous son, William Wallace. > Thursday rant over, I'm away home. > Ian. > > Paisley designs were certainly perfected and long manufactured in the > Scots town of Paisley, > in the mid 19th century. > > The basic "Paramecium paisleyi" motif is however of southern Asian origin, > and antedates > the 19th century. It seems to have originally depicted a drooping plant or > tree. > > Transitional forms between the drooping plant and the fully developed > Paramecium paisleyi > are still to be seen in tribal rugs and fabrics - I've assembled a > collection of photos > of them in the last few years. > > The fully developed Paramecium paisleyi reminds me of those things that > appear in my > eyes right before a migraine attack, or perhaps to one of those > pharmacologic states of > mind some of us listened to Sergeant Pepper's Lonely Hearts Club Band to, > back when that was new... > > The woods of Arcady are dead, > and over is their antique joy... (Yeats) > > Bob Richmond > Samurai Pathologist > Knoxville TN > > > ________________________________________________________________________ > AOL now offers free email to everyone. Find out more about what's free > from AOL at AOL.com. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Thu Jun 21 18:16:05 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Jun 21 18:16:10 2007 Subject: [Histonet] Bielschowsky/PAS Message-ID: A colleague of mine is having trouble with the Bielchowsky. It seems the silver is removed after oxidation in the 1% periodic acid for the PAS counter stain. Any suggestions? Thank you. Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu From jqb7 <@t> CDC.GOV Fri Jun 22 04:45:06 2007 From: jqb7 <@t> CDC.GOV (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Fri Jun 22 04:45:19 2007 Subject: [Histonet] Microwave Processing In-Reply-To: <1c4db3750706211117r3b9fa16djede227bbca314fb6@mail.gmail.com> References: <1c4db3750706211117r3b9fa16djede227bbca314fb6@mail.gmail.com> Message-ID: <34BB307EFC9A65429BBB49E330675F7201B0B874@LTA3VS003.ees.hhs.gov> We have the Sakura Xpress and like it very much. Email directly if you have specific questions or concerns. Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawn Bugge Sent: Thursday, June 21, 2007 2:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microwave Processing I was wondering if anyone had some pros and cons for microwave processing. We use the VIP processor now but are looking into microwave processing. Haven't found much information on the internet about what people really think about these products. Thanks for any information you have. -- Dawn R Bugge Seattle Histology Laboratory _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Jun 22 05:18:54 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Jun 22 05:19:05 2007 Subject: [Histonet] Small cell carcinoma Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222E778@wahtntex2.waht.swest.nhs.uk> When you weren't able to differentiate a small cell carcinoma from a lymphoma? Granted it means that your cytological skill is not what it should be but then everyone's not as good as you and me! Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; "The true meaning of life is to plant trees, under whose shade you do not expect to sit. --Nelson Henderson This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From scott.parker <@t> bio.usyd.edu.au Fri Jun 22 06:15:17 2007 From: scott.parker <@t> bio.usyd.edu.au (Scott Parker) Date: Fri Jun 22 06:12:34 2007 Subject: [Histonet] PLEASE UNSUBCRIBE GOING ON HOLIDAY Message-ID: <000601c7b4be$9d02c9a0$5ab14e81@wallacedom.bio.usyd.edu.au> Please unsubscribe me as I will be away on Holiday. Cheers, Scott Parker From Anthony.White <@t> vision-bio.com Fri Jun 22 10:11:14 2007 From: Anthony.White <@t> vision-bio.com (Anthony White) Date: Fri Jun 22 10:18:09 2007 Subject: [Histonet] please unsubscribe In-Reply-To: <000301c7b254$168b8db0$6424d182@IBLS.GLA.AC.UK> Message-ID: Anthony White Product Marketing Manager Vision BioSystems Inc. 1833 Portola Road Ventura CA 93003 United States of America telephone: +1 800 753 7264 facsimile: +1 781 616 1193 cell: +1 781 626 1604 www.vision-bio.com IMPORTANT - This email and any attachments may be confidential. Any retransmissions, dissemination or other use of these materials by persons or entities other than the intended recipient is prohibited. If received in error, please contact us and delete all copies. Before opening or using attachments, check them for viruses and defects. Our liability is limited to resupplying any affected attachments. [Any representations or opinions expressed in this email are those of the individual sender, and not necessarily those of Vision Systems Limited]. From emilie.belanger.trudelle <@t> umontreal.ca Fri Jun 22 10:31:25 2007 From: emilie.belanger.trudelle <@t> umontreal.ca (=?iso-8859-1?Q?B=E9langer_Trudelle_=C9milie?=) Date: Fri Jun 22 10:31:31 2007 Subject: [Histonet] unsuscribe Message-ID: Please unsuscribe me. Emilie B?langer Trudelle Universit? de Montr?al 2900 Boul. Edouard-Montpetit Pavillon Roger-Gaudry H3T 1J4 tel: (514) 343-6111 ext.3169 From anh2006 <@t> med.cornell.edu Fri Jun 22 11:02:23 2007 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Fri Jun 22 11:02:29 2007 Subject: [Histonet] donkey serum Message-ID: Does anyone use donkey serum from another source besides Jackson ImmunoResearch and have excellent results? Their serum is so costly for high volume IHC. Thanks, Andrea -- From ree3 <@t> leicester.ac.uk Fri Jun 22 11:09:07 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Fri Jun 22 11:09:15 2007 Subject: [Histonet] donkey serum In-Reply-To: References: Message-ID: SEROTEC do donkey serum................... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea Hooper Sent: 22 June 2007 17:02 To: Histonet Subject: [Histonet] donkey serum Does anyone use donkey serum from another source besides Jackson ImmunoResearch and have excellent results? Their serum is so costly for high volume IHC. Thanks, Andrea -- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trinimaican2501 <@t> yahoo.com Fri Jun 22 11:28:51 2007 From: trinimaican2501 <@t> yahoo.com (I-sanna Gibbons) Date: Fri Jun 22 11:28:55 2007 Subject: [Histonet] 2% celloidin Message-ID: <20070622162851.90042.qmail@web50312.mail.re2.yahoo.com> Hi everyone, How do I make up 2% celloidin with a solution referred to as collodion (nitrocellulose, ether, alcohol etc.)? I was told the solvent has to be Ether and Absolute Alcohol; but what is the ratio? Thanks I-sanna --------------------------------- Get the free Yahoo! toolbar and rest assured with the added security of spyware protection. From claycal44 <@t> yahoo.com Fri Jun 22 11:29:03 2007 From: claycal44 <@t> yahoo.com (nancy lowen) Date: Fri Jun 22 11:29:07 2007 Subject: [Histonet] CD31 Message-ID: <603171.20436.qm@web60411.mail.yahoo.com> I am working on CD31 IHC antibody staining on rat femurs and I was wondering what other people in Histoland consider to be the best antibody available for this. I have tried a couple from companies that I was not totally satisfied with. Any help would be appreciated. Thanks, Nancy Lowen claycal44@yahoo.com ____________________________________________________________________________________ Be a better Globetrotter. Get better travel answers from someone who knows. Yahoo! Answers - Check it out. http://answers.yahoo.com/dir/?link=list&sid=396545469 From gcallis <@t> montana.edu Fri Jun 22 11:29:43 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Jun 22 11:29:52 2007 Subject: [Histonet] donkey serum In-Reply-To: References: Message-ID: <6.0.0.22.1.20070622102042.01b4a6a8@gemini.msu.montana.edu> Andrea, I "Googled" donkey serum and Biocompare came up with many sources - 500 ml from Biomeda Corp for $90; Equitech-Bio 500 ml for $55. We buy large volumes of serum (goat 500 ml) realiquot upon arrival - in 50 ml centrifuge tubes, freeze down and store at -27C. Sigma might even have it but you pay the price of shipping. I don't know if price reflects quality, but it certainly is cheaper than the lyophilized but excellent serum from Jackson. Have a nice weekend. At 10:02 AM 6/22/2007, you wrote: >Does anyone use donkey serum from another source besides Jackson >ImmunoResearch and have excellent results? Their serum is so costly for >high volume IHC. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From rsrichmond <@t> aol.com Fri Jun 22 13:36:49 2007 From: rsrichmond <@t> aol.com (rsrichmond@aol.com) Date: Fri Jun 22 13:37:00 2007 Subject: [Histonet] Re: Small cell carcinoma In-Reply-To: <200706221301.7f7467c00513c7@rly-yj02.mx.aol.com> References: <200706221301.7f7467c00513c7@rly-yj02.mx.aol.com> Message-ID: <8C983136DBD4C3A-3F8-B836@WEBMAIL-DC12.sysops.aol.com> Well, at the age of 68 I've called a lot of small cell carcinomas without the benefit of immunohistochemistry. Having said that - the therapeutic stakes are just too high to forego any help you can get. Usually either synaptophysin and chromogranin will mark the tumor well enough to make the call. Some folks are adding CD56, with which I have no experience. Neuron specific enolase (NSE) is probably obsolete. A broad spectrum cytokeratin stain such as AE1/AE3 helps make sure it's an epithelial tumor, TTF-1 is of some help in assuring its lung, and a negative CD45 (Leukocyte Common Antigen, LCA) pretty much excludes lymphoma in this diagnostic situation. The cost, both in money and in misery, is just too high to risk being wrong here. IHC is expensive, but a lot less expensive than the wrong treatment. Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From mtitford <@t> aol.com Fri Jun 22 13:45:10 2007 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Fri Jun 22 13:45:26 2007 Subject: [Histonet] Celloidin time Message-ID: <8C9831498D3E35E-544-19143@WEBMAIL-DC11.sysops.aol.com> I-sanna somewhere asks about celloidin preparation: Celloidin is made up in 50% absolute alcohol, 50% ether. When preparing it though, it is best to let the dry celloidin stay in the absolute alcohol for 24 hours, and then add the ether. It dissolves faster that way. Celloidin is frightfully expensive these days, where did you purchase yours from? Michael Titford Pathology UDA Pathology Mobile AL USA ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From liz <@t> premierlab.com Fri Jun 22 15:42:19 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Jun 22 15:42:23 2007 Subject: [Histonet] MAC387 and multi nucleated giant cells Message-ID: Hello all =20 Does anyone out there know if MAC387 (L1 or calprotectin molecule) = stains multi nucleated giant cells. I did a quick internet search and = did not come up with anything definitive. I did find out that CD68 does = stain multi nucleated giant cells, but does MAC387? =20 Thanks in advance =20 Liz =20 Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 HYPERLINK "mailto:liz@premierlab.com"liz@premierlab.com HYPERLINK "http://www.premierlab.com/"www.premierlab.com =20 Ship to Address: =20 Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 =20 No virus found in this outgoing message. Checked by AVG Free Edition.=20 Version: 7.5.472 / Virus Database: 269.9.4/860 - Release Date: 6/21/2007 = 5:53 PM =20 From goinghisto <@t> hotmail.com Fri Jun 22 17:40:31 2007 From: goinghisto <@t> hotmail.com (Kathleen Crawford) Date: Fri Jun 22 17:40:42 2007 Subject: [Histonet] ] PLEASE UNSUBCRIBE In-Reply-To: <000601c7b4be$9d02c9a0$5ab14e81@wallacedom.bio.usyd.edu.au> Message-ID: Please unsubscibe my name from histonet Kathleen A. Crawford 5 Wingate Court Bangor, Maine 04401 Thank You! _________________________________________________________________ Like puzzles? Play free games & earn great prizes. Play Clink now. http://club.live.com/clink.aspx?icid=clink_hotmailtextlink2 From lpwenk <@t> sbcglobal.net Fri Jun 22 20:39:59 2007 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Fri Jun 22 20:40:15 2007 Subject: [Histonet] Vacation Unsubscribe Message-ID: <000501c7b537$6a3b04f0$0202a8c0@HPPav2> A summertime reminder of how to unsubscribe from Histonet when going on vacation. Go to link below http://lists.utsouthwestern.edu/mailman/listinfo/histonet Scroll to bottom of page. Click on "unsubscribe" and follow directions. When returning back from vacation, go back to link, and subscribe again. Have a nice vacation, whenever and wherever you go. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 From koellingr <@t> comcast.net Sat Jun 23 07:15:50 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Sat Jun 23 07:15:57 2007 Subject: [Histonet] donkey serum/many other sera Message-ID: <062320071215.9126.467D0EF600092CF0000023A622058863609D09020704040A0105@comcast.net> Andrea, You might want to try a place whose website is at www.Bioreclamation.com. Besides any standard (mouse, rat, human, etc) serum, they also have odd things like sera from beagle, guinea pig, donkey, ferret, cyno monkey, baboon, etc, etc. Can usually get it whole blood, serum, plasma or any kind of special order. I know many large pharmaceutical companies use them. Products are excellent. But to answer your concern about price comparisons with other companies, I have no idea at all since fortunately or unfortunately, that was not a concern in a former professional life. They are in a 516 telephone area code but providing serums and fluids like that is their main product and purpose so you might check out prices there. Again, products are excellent. Ray Koelling Phenopath Labs Seattle, WA -------------- Original message -------------- From: "Andrea Hooper" > Does anyone use donkey serum from another source besides Jackson > ImmunoResearch and have excellent results? Their serum is so costly > for high volume IHC. > > Thanks, > Andrea > -- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sohail_e <@t> yahoo.com Sat Jun 23 08:42:30 2007 From: sohail_e <@t> yahoo.com (sohail ejaz) Date: Sat Jun 23 08:42:33 2007 Subject: [Histonet] Immunohistochemistry with whole mouse embryos, Message-ID: <862390.44244.qm@web39509.mail.mud.yahoo.com> Hi, Gayle, While searching the histonet archive for information about processing whole mount mouse embryo, I came across your request regarding difficulcity in permiablization during Immunohistochemistry with whole mouse embryos, I have exactly the same problem that hhave encountered while staining E9.5 and E10.5 embryos. So i would like to enquire about some tips regarding permeabilization of embryos. I am using follwoing pattern 5% H2O2 in methanol for 5 hrs (Primary antibody) Rat antimouse CD31, Cat log No. 550274 1/50 (2ndry antibody) HRP conjugated affinipure goat anti Rat IgG code 112-035-167, 1/100 Jakson Immuno Research lab. Vector DAM, Sk 4100, Vector lab While --------------------------------- Park yourself in front of a world of choices in alternative vehicles. Visit the Yahoo! Auto Green Center. From sohail_e <@t> yahoo.com Sat Jun 23 08:47:30 2007 From: sohail_e <@t> yahoo.com (sohail ejaz) Date: Sat Jun 23 08:47:32 2007 Subject: [Histonet] permeabilize the embryos-Immunohistochemistry with whole mouse embryos Message-ID: <430078.10170.qm@web39514.mail.mud.yahoo.com> Dear Friends I am trying to stain whole mount of embryo E9.5 and E10.5 using undermentioned way, but i am unable to get any positive stainning to embryonic vasculature which might be a problem of permiabilization of embryo. So i would like to enquire about some chemicals and their exact concentrations which can be helpful to solve this issue. I am using follwoing pattern 5% H2O2 in methanol for 5 hrs (Primary antibody) Rat antimouse CD31, Cat log No. 550274 1/50 (2ndry antibody) HRP conjugated affinipure goat anti Rat IgG code 112-035-167, 1/100 Jakson Immuno Research lab. Vector DAM, Sk 4100, Vector lab While --------------------------------- Fussy? Opinionated? Impossible to please? Perfect. Join Yahoo!'s user panel and lay it on us. From adesupo2002 <@t> hotmail.com Sat Jun 23 09:12:31 2007 From: adesupo2002 <@t> hotmail.com (ADESUPO ADESUYI) Date: Sat Jun 23 09:12:36 2007 Subject: [Histonet] CD 10 PROCEDURE Message-ID: Hi, How are you guys doing? pls i will appreciate it, if you guys could send me your procedure for running your CD 10 using Dako Antibody. We have been having problem with our CD 10 for some time now. Thanking you for your usual cooperation. Adesupo. _________________________________________________________________ Play free games, earn tickets, get cool prizes! Join Live Search Club.? http://club.live.com/home.aspx?icid=CLUB_wlmailtextlink From koellingr <@t> comcast.net Sat Jun 23 09:51:15 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Sat Jun 23 09:51:26 2007 Subject: [Histonet] RE: microglia in mouse brains Message-ID: <062320071451.15225.467D3362000C80C500003B7922007621949D09020704040A0105@comcast.net> Carl, I agree from what I understand that the BM8 is a clonal designation to the F4/80 antigen and complex. But then I ask not critically, but rhetorically, how can BM8 not be used to detect microglia and F4/80 can? I have used BM8 on mice microglia in paraffin as have others. But you can also see slightly different populations of other types of macrophages elsewhere depending on whether you use F4/80 or BM8, so I'm not sure if anyone has precisely pinned down this entire story, especially for IHC. I'm curious, if you tried BM8 on rat/mice brains with no staining, what was your procedure for the rat since BM8 is a rat IgG2a monoclonal? Rat on rat? Or a conjugate? I've not used, but only know a bit about Iba1(and its alternate names), through literature reading. But I must say that your pictures I see look compelling and great as far as I can visualize them. I suppose I'd be more convinced if I saw another consecutive section with another microglia marker (RCA-1 or something else) and it stained the same population. Sort of a "weight of evidence" thing. The phosphotyrosine picture I find much less compelling. It looks like a bunch of nuclear staining but I'm having trouble seeing the picture clearly. This is not directed at you but in general, I find phosphoprotein targets for IHC to be dubious. The phosphorylation of molecules as part of the signaling transduction pathway is by nature meant to be a very temporally limited activity. Your cells in general don't want its signaling molecules permanently phosphorylated. Indeed, if you do these studies by Westerns, you need to do them immediately, on ice and in the presence of phosphatase inhibitors. Otherwise, the phosphorylated target will be gone. So why should the target stick around in a piece of tissue that is not fixed in-situ and instantaneously at death? I know there are models utilizing axotomy in rodents to upregulate microglial phosphotyrosine activity. But it is my understanding that in controls, there is little to no p-tyr staining and only weak, upregulated p-ty r staining in the manipulated animals. If you look at the data sheets of every single phospho-signalling antibody out there, the IHC picture is always of a hunk of tumor where by definition, pathways are constitutively turned on and upregulated. So for tumor assesment in IHC, I think there is indeed a place for phospho-protein antibodies. The 4G10#05-321 you describe in the picture, if you look on the data sheet, the staining control they suggest for Westerns is a cell line A431, which is a human squamous cell carcinoma and then to be used after boiling so proteins are in a reduced state. I think your Iba1 stains look fantastic and compelling for what little I know about that antibody. The p-tyr, I'm really skeptical of but not having anything to do with you but concerning the whole concept of that kind of staining. But then I'm from Missouri, called the "Show Me", state so perhaps I tend to under-call rather than over-call IHC until I'm really, really convinced. Ray Koelling Phenopath Labs Seattle, WA -------------- Original message -------------- From: "Carl Hobbs" > I understand that the Antigen is designated as F4/80 and BM8 is but a clone > designation of a monoclonal against a part of the F4/80 Ag? > I tried BM8 on pwax rat/mouse brains and it was negative. Perhaps it does > not work in that particular application or, according to AbDSerotec, clone > BM8 is reported not to identify microglia, unlike the "original " Ab against > F4/80, clone C1:A3-1. > I have not yet tried the latter. > However, any comments regarding microglial staining in mouse/rat brains( > p.wax sections, after HIER) of Wako's Iba1 Ab 01-19741, would be > appreciated. > Please see here http://www.immunoportal.com/index.php > for pics. > Navigate to Image gallery and search for "microglia" and then "Wako", for > eg. > Also, I noted that an anti p-tyrosine Ab ( p-tyr) seems to be highly > selective for microglia, in the same application; ( please see same > site/Image gallery for pics, also. > Any expert comments on these pics would be most welcome. > If there are any, perhaps you would be kind enough to post your comments in > that forum, also? > I am a "jack-of-all trades" Histologist/Immunohistologist , rather than an > expert in any particular field. > Carl > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Sat Jun 23 15:21:22 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Sat Jun 23 15:21:58 2007 Subject: [Histonet] Re Microglia Message-ID: <004501c7b5d4$1174a5e0$4001a8c0@carlba65530bda> I agree from what I understand that the BM8 is a clonal designation to the F4/80 antigen and complex. But then I ask not critically, but rhetorically, how can BM8 not be used to detect microglia and F4/80 can?( Carl: As I stated, BM80 is an Ab against the designated F4/80 antigen.One cannot use F4/80...it is an Ag, not an Ab) I have used BM8 on mice microglia in paraffin as have others( Carl: OK..I just cannnot make it work for me, on Pwax sections of rat/mouse HIER-sections). But you can also see slightly different populations of other types of macrophages elsewhere depending on whether you use F4/80 or BM8, so I'm not sure if anyone has precisely pinned down this entire story, especially for IHC( Carl: rhetorically, has anyone? Also, you use the term "slightly"..I am not sure what you mean by this...). I'm curious, if you tried BM8 on rat/mice brains with no staining, what was your procedure for the rat since BM8 is a rat IgG2a monoclonal? Rat on rat? Or a conjugate?( Carl: I am not sure what you mean......I use mouse on mouse, rat on rat etc with no problems, on pwax sections. You have looked at the Immunoportal website...the pictures speak for themselves, surely? Also, there is no such thing as "slightly different populations": they are different or not. You use an oxymoron ;-) I've not used, but only know a bit about Iba1(and its alternate names), through literature reading. ( Carl: I am surprised. I would be grateful for it's alternative name) But I must say that your pictures I see look compelling and great as far as I can visualize them( Carl: I can send to you any pics, of higher quality, of Iba1 that you need ). I suppose I'd be more convinced if I saw another consecutive section with another microglia marker (RCA-1 or something else) and it stained the same population. Sort of a "weight of evidence" thing. ( Carl: sure....I agree. "weight -of-evidence " is also my "philosophy". Please give me details of suppliers of "RCA-1" and "or something else". Also.....can anyone direct me to sites that have ImmunoDAB pics of FFPW sections of microglia?) The phosphotyrosine picture I find much less compelling. It looks like a bunch of nuclear staining but I'm having trouble seeing the picture clearly( Carl: that is clearly untrue: Yes, there appears to be nuclear staining but there is also CLEAR staining of processes....if anyone else has looked at the pic/s please add your comments). This is not directed at you but in general, I find phosphoprotein targets for IHC to be dubious. The phosphorylation of molecules as part of the signaling transduction pathway is by nature meant to be a very temporally limited activity. Your cells in general don't want its signaling molecules permanently phosphorylated. Indeed, if you do these studies by Westerns, you need to do them immediately, on ice and in the presence of phosphatase inhibitors. Otherwise, the phosphorylated target will be gone. So why should the target stick around in a piece of tissue that is not fixed in-situ and instantaneously at death? I know there are models utilizing axotomy in rodents to upregulate microglial phosphotyrosine activity. But it is my understanding that in controls, there is little to no p-tyr staining and only weak, upregulated p-ty( Carl: OK...lol, you state the accepted, theoretical line. So, what is the anti p-Tyr staining in my cell processes due to? That was one of my questions. If one does not have answers....;-) r staining in the manipulated animals. If you look at the data sheets of every single phospho-signalling antibody out there, the IHC picture is always of a hunk of tumor where by definition, pathways are constitutively turned on and upregulated. So for tumor assesment in IHC, I think there is indeed a place for phospho-protein antibodies. The 4G10#05-321 you describe in the picture, if you look on the data sheet, the staining control they suggest for Westerns is a cell line A431, which is a human squamous cell carcinoma and then to be used after boiling so proteins are in a reduced state.( Carl: what do you mean by Boiling? For sections, or Westerns?) ( Carl: hmmmm. So, for eg, why can we easily see Ki67 positivity? It has a 1/2 life approx 30mins....Most people seem to have no problems with detecting that Ag. I have no probs with GOOD p-Abs. I can show you more pics if you wish. Let us not forget that affinity of Ab to Ag is very important. Sure, it is a transient occurence and cannot be quantified, immunohistochemmically, but....at any point in time, there will be positivity, immunohistochemically.( it's the law...according to Rosencrantz and Guildenstern.....;-) I think your Iba1 stains look fantastic and compelling for what little I know about that antibody. The p-tyr, I'm really skeptical of but not having anything to do with you but concerning the whole concept of that kind of staining. But then I'm from Missouri, called the "Show Me", state so perhaps I tend to under-call rather than over-call IHC until I'm really, really convinced.( Carl: hey Friend. I reckon that you are what we Angles call: "bullshitting"? Me too ..I have no concrete support that my results are.....sigh, real.) Thanks for your answer, Ray.Appreciated! I reckon that you are equally in the dark, as they say ;-) Carlos The Clueless From NPLASTER <@t> triad.rr.com Sun Jun 24 15:23:20 2007 From: NPLASTER <@t> triad.rr.com (NPLASTER@triad.rr.com) Date: Sun Jun 24 15:23:26 2007 Subject: [Histonet] please unsubsubscribe Message-ID: nancy plaster 6 stafford oaks dr kernersville nc From amosbrooks <@t> gmail.com Sun Jun 24 15:58:11 2007 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sun Jun 24 15:58:16 2007 Subject: [Histonet] Claudin 20 Message-ID: <582736990706241358n234ccaa9g11f48cc31b41d6dc@mail.gmail.com> Hi, Has anyone worked with Claudin 20 for immunohistochemistry (IHC)? I have a researcher interested in using this antibody (from Abnova) for human formalin fixed paraffin embedded tissues, probably microarrays. The spec sheet is not particularly helpful, I don't think it is intended (or tested) for IHC as there is not much information. I believe the current concentration is 1mg/mL. I assume epitope retrieval with heat will be necessary. I will probably also contact the company tech support but any suggestions from people that hace any good ideas would be more than welcome. Thanks in advance, Amos Brooks From talulahgosh <@t> gmail.com Sun Jun 24 17:34:08 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Sun Jun 24 17:34:13 2007 Subject: [Histonet] donkey serum In-Reply-To: <6.0.0.22.1.20070622102042.01b4a6a8@gemini.msu.montana.edu> References: <6.0.0.22.1.20070622102042.01b4a6a8@gemini.msu.montana.edu> Message-ID: Equitech Bio is definitely the way to go if you want to save on serum costs. We buy all of our serum from them. Their serum is the same quality as Jackson Immuno--we compared the two in an immunofluorescence experiment and both worked well. They have a minimum order of $100 and heat inactivation costs about $10 more than the listed price. We aliquot the extra and freeze it at -20C, it lasts us about a year. Emily -- penguins, spoons, and you--what's life like among the flightless? http://www.getafirstlife.com/ From koellingr <@t> comcast.net Sun Jun 24 20:47:18 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Sun Jun 24 20:47:26 2007 Subject: [Histonet] microglia/philosophy OT Message-ID: <062520070147.17409.467F1EA6000234E40000440122028887449D09020704040A0105@comcast.net> Is a very long answer to Carl as well as some philosophy so just hit delete right now if you have other matters or interests... Hi again Carl, So this is the ultimate demonstration of how e-mail and the internet cannot be used readily for true scientific discussion. The inexactitude of any statement anyone can make in an e-mail simply cannot stand up to the scrutiny of logic and reason in a final sense. People would have you almost believe that we are all so sophisticated now with e-mail questions and answers that something like a theory or thesis or journal article can just be submitted by e-mail and that is somehow the final and lasting story. This instrument, no matter what computer geeks say, was, is and will never meet the requirement of logical expression during dynamic interaction. Not meant for debate, disagreement or thorough discussion. I minored in math as an undergrad and actually I enjoy the certitude and precision of math sometimes more than I do science. Thus it is my lifelong hobby. To me, a complicated problem you intellectually solve in trigonometry is simply very relaxing and mind settling. If we were to discuss different opinions regarding the steps of integration of the complicated integrand in an integral calculus problem, back and forth by e-mail, we'd never reach a conclusion. It just can't be done. Besides e-mail not being able to solve such discrepancies, there is another problem. I assume John Kiernan is out there lurking somewhere although I don't remember seeing him for a while and I certainly don't put my brain anywhere near the same intellectual category as his. But I believe it was he who has cautioned over and over about believing what is said on the Histonet, simply because someone wrote it. I guess that is true when you think about it. After all, it is certainly possible that in reality I am a disgruntled moonshiner, deep in the Ozark Mountains of the "Show Me" state and doing my best to stay hidden from the revenooers. My shack has no electricity and I run my laptop off an old solar panel when I'm not guarding the still with my 12 gauge shotgun. I might have never been in a histo/patho/immunology lab in my life so my answers and thoughts might be pure hogwash. In any event, I'll try to clarify a bit more what I believe I said and follow-up on some of your questions. To pin this down with more precision, we'd have to wait until I make it to the UK and we can sit for an afternoon with some pitchers of ale to discuss it. Or you can get over here, and we will sit for the day while sharing some of my 180 proof hootch. My place is 5 miles just over the ridge from Jed Clampetts old shack. 1) Yes F4/80 is an antigen but indeed there are F4/80 antibodies (that are not BM8 clones). Whether rightly or wrongly called, there are antibodies called F4/80. They exist and because of my thesis project I commonly used an antibody called F4/80. I knew what it was but catalogs are full of such a designation. 2) the term "slightly". Use F4/80 antibody and BM8 antibody on flow in spleen, lymph node and other secondary lymphoid organs. Gate out populations and see "slight" differences in percentages and when you back-gate. Do single color histograms, overlay, and see unexplained "shoulders" or spread out peaks. Sort them on cell sorters onto microscope slide and see very similar but "slightly" different cells. So that is what I mean by "slightly". By pure histology and IHC, visual recognition sensitivity might be below detectable limits but I do not believe the populations to be "the same". So "slightly different". Sometimes oxymorons convey the most precise definition. (3) I agree with the picture again that as far as my resolution goes, it looks commendable for the non p-tyr pictures. I asked about rat-on-rat for as far as I can remember, people have always struggled with the concept of an antibody raised in a host being placed on the same species tissue. Looking at that antibody then with detection against the antibody brings on the problem of picking up endogenous tissue Ig. Everyone I know frets with the problem of using mouse hosted antibodies on mouse tissues, rat hosted antibodies on rat tissues and human backbone configured antibodies on human tissue. If you very easily and readily use a rat antibody (like BM8) on rat tissue, or mouse Ab on mouse or human Ab on human, I'd sure like to know the details and I'm sure the entire IHC world would to. (4) iba1 = ionized calcium binding adapter molecule. It doesn't have an alternative name. It has about 15 alternative names, interferon gamma responsive transcript (irt1), allograft inflammatory factor 1 (AIF1). You can find a host of others I can't possibly remember on a gene search or antibody search or some data sheets. (5)No need to send pictures. I absolutely believe what you say is there and you see staining. I take it as axiom. RCA-1 is ricinus communis agglutinin-1, a lectin. Can get unconjugated, biotin, perox and I usually go to Vector Labs. At first I was going to say I made an error and that RCA-1 could only be used on human microglia but a few papers I see uses RCA on mouse microglia. And if you are in rat there are the famous OX series OX-41,42,11 that stain microglia but others know much more about them than I do. See Bertolotto, JHC vol 41#4 481-487 1993, see Mason, J AJP 164 #5 May 2004 on Oligodendrocytes and precursers, see Hematopoietic Prostaglandin D Synthetase is expressed in microglia in mouse brain, Mohri,I in GLIA 42:263-274 (2003) for frozen, paraffin, EM of microglia staining with "other" antibodies. I found them in 4-5 minutes in PubMed and I HATE, HATE, HATE computers (except for Power Point, word processing and data entry to Excel). All the information is out t here with picture s and alternative markers in abundance. (6)What is the anti p-tyr staining due to? Haven't the slightest idea. Background, false signal, smudge, true signal on inappropriate epitope, 50 other things. One possibility is that it is exactly what you think it is. True anti p-Tyr staining you are looking for. All I'm saying is this. When I look for a membrane bound or housekeeping, constiuitive target (CD4 on T-helper cells, cytokeratins in epithelial cells, etc, etc) that have been characterized around the world in thousands of labs over and over and by myself, when I see the stain and knowing where it should be and shouldn't be in absolute terms, my confidence level is as absolute as is possible that I am seeing true staining. But the whole concept of IHC staining for phosphoylated targets is very new and very tricky. In grad school and in research, I have had opportunity to parse apart several different signal transduction pathways and looking at the phosphorylated intermediates. Used westerns, lysates, cell line s, stimulated cell lines, flow, tissue IHC (by perfusion/paraffin, frozen and dunking into formalin and paraffin) and trying to make concordance with all the data to what I saw under a microscope. While I absolutely agree I found antibodies that MIGHT work as well in IHC as in more standardized molecular techniques, I am still very, very wary of what I see on phospho-protein targeting antibodies on a tissue section. To me, this does not have so much to do with affinity as with specificity. I've looked at signaling transduction proteins in the cascade that were phosphorylated say at sites tyr82, tyr99, tyr118, tyr173 and tyr176. Sometimes individually or alone, or in combinations or all at once and for only a brief few minutes before down-regulating. That is pretty intricate and tough detection science to put on an antibody used in IHC, no matter what a commercial company says or what is the avidity or affinity of the antibody. In short, your staining maybe absolutely real fo r p-tyr. I just can't hang my hat on it without further proof than that something is turning dark brown. (7) The data sheet I accessed from the information uses that p-tyr on Westerns. And they themselves state to boil (as in with heated water or as in soap as in SDS-PAGE) to reduce the protein. That in itself says to me that this target could possibly be a buried, short epitope that needs to be linearized as opposed to an exposed conformational epitope that you can see more naturally (like in a tissue section). If you get hold of them and they tell me you can detect using their antibody on Westerns but can run the gel in a "non-reduced" state, I'm more likely to believe it to be good for standard IHC. Not totally convinced but would ease my mind a bit. (8) Ki-67 does have a short half-life but that cell is not going to be in G1,S,G2 and M very long anyway. Absent from G0, in which that cell might have been for extraordinarily long periods, once the molecules and machinery get the go signal to enter G1, that proliferation goes quickly but assuredly. Entering into anaphase and telophase is toward the end of that proliferation. So yes it is there for a short period, less than an hour, but then it is actually there. Even if you take a half life as 1/2 hour, half is still left after 1/2 hour. And that is a lot. And remember that Ki-67 is in nucleus. Take a "little" of something and concentrate it in a small dot (nucleus) and it might be easy to visualize. Take a "little" of something and spread it out over the whole volume of a cytoplasmic process and it might not be so easy to see. I am not sure I agree with you that at any point there must be positivity. Cells were set up, at least in this instance to respond to small amou nts of phophorylation. So the entire protein milieu does not have to be phosphorylated. Just a little. And if that phosphorylation lasts 1 or 2 minutes before de-phosphorylation, I just don't think it is enough to reliably detect. That is except, as I said before, in the case of tumors, which can have phosphorylation cascades permanently turned on by definition. Notwithstanding Dr Rosencrantz and Dr. Guilensterns ideas on a game of spinning coins --as others have said "the law of probability has no jurisdiction here". And their learned conversations have been noted to be "quite often a series of non-sequitors". Remember, this is just one ordinary Histo-grunts opinion so I think I'm done now and besides, I believe I hear a durn revenooer nosin down by where the still is. Good luck with your project. Ray Koelling Phenopath Labs Seattle, WA From joost.bruijntjes <@t> tno.nl Mon Jun 25 06:59:07 2007 From: joost.bruijntjes <@t> tno.nl (Bruijntjes, J.P. (Joost)) Date: Mon Jun 25 07:02:42 2007 Subject: [Histonet] (no subject) Message-ID: <8865601DD17A554CB489C17FFD8A51B20E9C67@MAIL04.tsn.tno.nl> Hi all I am searching for an antibody directed against human macrophages in formalin fixed and paraffin embedded tissues. Up to now I found a CD68 and a CD163, both reacting with macrophages. Does anyone of you know other markers? Thanks in advance. Joost Bruijntjes TNO Quality of Life Zeist The Netherlands TNO.NL Joost Bruijntjes T +31 30 694 44 80 F +31 30 694 49 86 E joost.bruijntjes@tno.nl Disclaimer This e-mail and its contents are subject to the DISCLAIMER at http://www.tno.nl/disclaimer/email.html From ryakay <@t> shands.ufl.edu Mon Jun 25 07:28:21 2007 From: ryakay <@t> shands.ufl.edu (Kaye Ryan) Date: Mon Jun 25 07:29:05 2007 Subject: [Histonet] Microwave Processing In-Reply-To: <1c4db3750706211117r3b9fa16djede227bbca314fb6@mail.gmail.com> References: <1c4db3750706211117r3b9fa16djede227bbca314fb6@mail.gmail.com> Message-ID: <467F7CA5020000D80000A02F@gw-fs1.shands.ufl.edu> Hi Dawn, We have been using the Milestone RHS1 units for over four years now and absolutely love them. We actually have three of them. I can tell you from first hand experience that it is wonderful for TAT, cutting and saving on processing reagents. We can receive, process, embed and be ready to cut in just a little over an hour. The blocks cut so much better than routine processing and you don't get the chatter that you normally get when cutting gastric biopsies. The tissues also lay very flat. We have purchased the fully automated Milestone PATHOS and have been testing the system for several months. We are just about ready to convert over to fully processing all tissues with this system. We have tested tiny biopsies with large colon and breast samples and they have turned out beautifully. The nuclear detail is great and it is wonderful for our IHC department. We expect to see a huge savings on reagents and paraffin once we convert. We now have our overnight processing down to 5 and ? hours. As you can tell, I am sold on the technology. Hope this helps, Kaye Kaye Ryan Histology Manager/Educational Coordinator Shands Rocky Point Laboratories (352) 265-0111, 72093 >>> "Dawn Bugge" 6/21/2007 2:17 PM >>> I was wondering if anyone had some pros and cons for microwave processing. We use the VIP processor now but are looking into microwave processing. Haven't found much information on the internet about what people really think about these products. Thanks for any information you have. -- Dawn R Bugge Seattle Histology Laboratory _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From derek.papalegis <@t> tufts.edu Mon Jun 25 08:16:02 2007 From: derek.papalegis <@t> tufts.edu (Derek Papalegis) Date: Mon Jun 25 08:16:07 2007 Subject: [Histonet] HT exam Message-ID: <467FC012.9070101@tufts.edu> Does anybody know the score range of the HT exam? I just found out what my actual score was and I want to see where I fall in that range. Thanks, Derek -- Derek Papalegis HT (ASCP) Histotechnician Division of Laboratory Animal Medicine Tufts University 136 Harrison Avenue Boston, MA 02111 phone: 617 636-2971 fax: 617 636-8354 From cmiller <@t> physlab.com Mon Jun 25 08:55:18 2007 From: cmiller <@t> physlab.com (Cheri Miller) Date: Mon Jun 25 08:55:28 2007 Subject: [Histonet] HT exam In-Reply-To: <467FC012.9070101@tufts.edu> References: <467FC012.9070101@tufts.edu> Message-ID: <002401c7b730$7921a5f0$3402a8c0@plab.local> Isn't it 400 - 600 Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Derek Papalegis Sent: Monday, June 25, 2007 8:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT exam Does anybody know the score range of the HT exam? I just found out what my actual score was and I want to see where I fall in that range. Thanks, Derek -- Derek Papalegis HT (ASCP) Histotechnician Division of Laboratory Animal Medicine Tufts University 136 Harrison Avenue Boston, MA 02111 phone: 617 636-2971 fax: 617 636-8354 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From aboris <@t> agh.org Mon Jun 25 09:09:57 2007 From: aboris <@t> agh.org (Anthony F. Boris) Date: Mon Jun 25 09:07:09 2007 Subject: [Histonet] HT exam References: <467FC012.9070101@tufts.edu> Message-ID: <5D942CF4AE61B24E8DDF0DE22F2827551FEA07@mse2.agh.org> Try this link for detailed score info from the ASCP BOR. http://www.ascp.org/Certification/News/newsletter/Newsletter_Spring2007.pdf -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Derek Papalegis Sent: Mon 6/25/2007 9:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT exam Does anybody know the score range of the HT exam? I just found out what my actual score was and I want to see where I fall in that range. Thanks, Derek -- Derek Papalegis HT (ASCP) Histotechnician Division of Laboratory Animal Medicine Tufts University 136 Harrison Avenue Boston, MA 02111 phone: 617 636-2971 fax: 617 636-8354 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From derek.papalegis <@t> tufts.edu Mon Jun 25 09:33:32 2007 From: derek.papalegis <@t> tufts.edu (Derek Papalegis) Date: Mon Jun 25 09:33:39 2007 Subject: [Histonet] HT exam In-Reply-To: <002401c7b730$7921a5f0$3402a8c0@plab.local> References: <467FC012.9070101@tufts.edu> <002401c7b730$7921a5f0$3402a8c0@plab.local> Message-ID: <467FD23C.20809@tufts.edu> Cheri Miller wrote: > Isn't it 400 - 600 > > Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Derek > Papalegis > Sent: Monday, June 25, 2007 8:16 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] HT exam > > Does anybody know the score range of the HT exam? I just found out what > my actual score was and I want to see where I fall in that range. > > Thanks, > Derek > > 400 is passing but I am not sure what a perfect score would be. I know it is higher than 600 however. -- Derek Papalegis HT (ASCP) Histotechnician Division of Laboratory Animal Medicine Tufts University 136 Harrison Avenue Boston, MA 02111 phone: 617 636-2971 fax: 617 636-8354 From crculber <@t> uncc.edu Mon Jun 25 10:12:04 2007 From: crculber <@t> uncc.edu (Culberson, Cathy) Date: Mon Jun 25 10:12:24 2007 Subject: [Histonet] fixative for endothelial cell membrane for protein and lipid staining Message-ID: I need to fix primary liver endothelial cells to stain for cell membrane lipids and also for a membrane protein, caveolin. What fixative would be best for this? Thanks for your help, as always. Cathy Culberson, MS University of North Carolina at Charlotte Dept of Biology 704-687-8675 fax 704-687-3128 email crculber@uncc.edu From JWEEMS <@t> sjha.org Mon Jun 25 11:31:16 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Mon Jun 25 11:31:52 2007 Subject: [Histonet] de Galantha stain for Gout Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FF9C85@sjhaexc02.sjha.org> Anyone have this procedure? I can only find references - not the actual procedure. Need today if possible. Thanks! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From JWEEMS <@t> sjha.org Mon Jun 25 11:59:22 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Mon Jun 25 11:59:47 2007 Subject: [Histonet] de Galantha stain for Gout In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FF9C85@sjhaexc02.sjha.org> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FF9C90@sjhaexc02.sjha.org> Found it - thanks everyone! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Weems, Joyce Sent: Monday, June 25, 2007 12:31 PM To: Histonet Subject: [Histonet] de Galantha stain for Gout Anyone have this procedure? I can only find references - not the actual procedure. Need today if possible. Thanks! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From Eric.C.Kellar <@t> questdiagnostics.com Mon Jun 25 12:11:30 2007 From: Eric.C.Kellar <@t> questdiagnostics.com (Kellar, Eric C) Date: Mon Jun 25 12:11:43 2007 Subject: [Histonet] de Galantha stain for Gout Message-ID: <6843061CE6B98E4B96590D4F299618F801583C2E@qdcws0117.us.qdx.com> De Galantha's Method for Urate Crystals Fixation: Absolute alcohol (4 hours for 2mm thick specimens). Paraffin: From absolute alcohol transfer blocks to xylene-paraffin, equal parts, at 58C for 2 hours. Place in paraffin for 1 hour. Embed. Microtomy: Cut sections at 8 microns. Developing solution: 3% Gelatin in hot water 10 ml 10% Silver Nitrate 3 ml 2% Hydroquinone 2 ml Prepare just before use. 1. Place slides in xylene, then absolute alcohol, 2 changes each. Avoid water. 2. Place in 10% silver nitrate solution. Expose to strong sunlight until urates are a bright rose; approximately 3 hours. 3. Prepare developing solution and pour over slides until the urates turn black and connective tissue is yellow. 4. Wash slides quickly in hot water 58C. 5. Dehydrate slides in absolute alcohol, three changes, clear and mount. Results: Urates Black Background Yellow Reference: Histopathological Methods and Color Atlas of Special Stains and Tissue Artifacts, Lee Luna 1992. Eric C. Kellar Quest Diagnostics Histology Laboratory Supervisor South Florida -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Weems, Joyce Sent: Monday, June 25, 2007 12:31 PM To: Histonet Subject: [Histonet] de Galantha stain for Gout Anyone have this procedure? I can only find references - not the actual procedure. Need today if possible. Thanks! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From talulahgosh <@t> gmail.com Mon Jun 25 13:24:23 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Mon Jun 25 13:24:27 2007 Subject: [Histonet] what do people for ISH In-Reply-To: References: Message-ID: The important things to remember are -always use DEPC water (1 mL DEPC / 1 L water, then autoclave for 30 min) -always bake glassware, utensils, anything not plastic (we bake overnight at 250F) and autoclave plastic -always use gloves when handling anything RNase free -RNase Away is your best friend (but cannot be used on metal) We use separate reagents for RNase free solutions, but some people seem to think that doesn't matter. In regards to starting the RNase free process, when you sacrifice the embryo, it itself has RNases, so making anything before this RNase free is futile. We consider our embryos RNase free after they have been fixed in 4% para (made in DEPC water, in baked bottles, aliquoted into sterile/new Falcon tubes). After this, every solution used is RNase free. Our cryostat is by default RNase free since you have to wear gloves because it's cold. If you use a microtome that isn't RNase free, just use a separate blade and wipe down whatever you use with RNase away. In the case of the glass staining dishes, which can't be baked, we rinse them with RNase Away and reuse the same dishes for each experiment. This way they are separated from not RNase free dishes, and we know they haven't been contaminated in the washing process. Emily -- penguins, spoons, and you--what's life like among the flightless? http://www.getafirstlife.com/ From Dorothy.L.Webb <@t> HealthPartners.Com Mon Jun 25 13:26:40 2007 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Mon Jun 25 13:26:50 2007 Subject: [Histonet] ASCP certification Message-ID: <0E394B648E5284478A6CCB78E5AFDA2703640DC6@hpes1.HealthPartners.int> I have 3 histotechs that still need to achieve their ASCP certification. Are there any practice exams out anywhere? One tech has been around for 15 years and is afraid she will not remember her theory enough to pass a current exam. Thanks ahead of time! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From jgutierrez <@t> precisionpath.us Mon Jun 25 13:40:43 2007 From: jgutierrez <@t> precisionpath.us (Juan Gutierrez) Date: Mon Jun 25 13:41:59 2007 Subject: [Histonet] Grossing dye mordant Message-ID: What can someone use to replace Bouin's as a dye mordant? I'm not looking for commercial stuff, but something I can make here in the lab. Thank you in advance for your replies. Juan C. Gutierrez, HT(ASCP) Precision Pathology (210)646-0890 From sjchtascp <@t> yahoo.com Mon Jun 25 13:43:51 2007 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Mon Jun 25 13:43:56 2007 Subject: [Histonet] Seeking Employment Message-ID: <694585.30847.qm@web38201.mail.mud.yahoo.com> 14 plus years in Histology. Seeking PT, as needed, contract in So.WI or No.Ill. Will do up to a 3 week assignment anywhere else.. I can re contacted at yelkaco@ticon.net for a resume and references. Thanks much everyone, Steve --------------------------------- Shape Yahoo! in your own image. Join our Network Research Panel today! From JCollins <@t> palmbeachpath.com Mon Jun 25 14:14:56 2007 From: JCollins <@t> palmbeachpath.com (Judy Collins) Date: Mon Jun 25 14:15:01 2007 Subject: [Histonet] ASCP Exam Message-ID: The NSH has a wealth of good educational material as well as practice question booklets. Also, ASCP BOR has a study guide with practice tests and they now have online practice tests which can be purchased. Judy Collins Palm Beach Pathology From erweber <@t> maxhealth.com Mon Jun 25 14:17:52 2007 From: erweber <@t> maxhealth.com (Eric Weber) Date: Mon Jun 25 14:15:57 2007 Subject: [Histonet] Histology Techs Needed Message-ID: <9D12D4EF30176D4F839EB47F3D0E8436C1631C@exbk2.maxhealth.com> Good Afternoon Everybody, I hope this email finds you well. My company is in need of three qualified histology techs for temporary assignments. If you are working travel or have considered travel work, please contact me. I would like to discuss the open positions we have available. Thank you in advance. Eric Weber Recruitment Specialist Maxim Staffing Solutions Toll Free (866) 466-2974 Toll Free Fax (866) 466-2981 erweber@maxhealth.com From carl.hobbs <@t> kcl.ac.uk Mon Jun 25 14:24:04 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Mon Jun 25 14:24:23 2007 Subject: [Histonet] Re: microglia/philosophy OT Message-ID: <000501c7b75e$651c97d0$4101a8c0@carlba65530bda> Well, Ray, your answer is worthy of rereading, digestion and cogitation before I am able to give you my reply which I hope is worthy of your eloquence in elaborating your point of view. Perhaps Cicero would have been proud of you.......? I will reply by personal email, as a very poor substitute of that very tempting offer to chew this cud over a few beers. However, if anyone else is willing to pitch in on the Histonet server.......I'm game. Yes..I too miss JKiernan's incisive comments, pearls of wisdom, words of caution ( to name a few of his admirable professional qualities). Carl From pruegg <@t> ihctech.net Mon Jun 25 14:39:56 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Jun 25 14:40:06 2007 Subject: [Histonet] fixative for endothelial cell membrane for protein andlipid staining In-Reply-To: Message-ID: <007201c7b760$9cf517c0$6501a8c0@Patsy> I would think that ordinary NBF would to the job. You might want to consider something other than paraffin processing though to preserve the lipids, perhaps formalin fixed, sucrose infiltrated frozen sections??? Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Culberson, Cathy Sent: Monday, June 25, 2007 9:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fixative for endothelial cell membrane for protein andlipid staining I need to fix primary liver endothelial cells to stain for cell membrane lipids and also for a membrane protein, caveolin. What fixative would be best for this? Thanks for your help, as always. Cathy Culberson, MS University of North Carolina at Charlotte Dept of Biology 704-687-8675 fax 704-687-3128 email crculber@uncc.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From david.kinsley <@t> spcorp.com Mon Jun 25 14:47:54 2007 From: david.kinsley <@t> spcorp.com (Kinsley, David) Date: Mon Jun 25 14:48:00 2007 Subject: [Histonet] Paraffin specimen clamp Message-ID: Hi, I cut paraffin sections on a Microm HM 355 microtome. I recently received some paraffin samples from Europe and they are rectangular paraffin blocks measuring approximately 32x22x22mm without any plastic cassette attached to them. Does anyone know where I may be able to purchase a different type of specimen holder/clamp that would hold the paraffin block directly without the need for a cassette? Unfortunately, I cannot melt the blocks and re-embed them with cassettes. thanks in advance Dave ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From froyer <@t> bitstream.net Mon Jun 25 15:13:13 2007 From: froyer <@t> bitstream.net (Ford Royer) Date: Mon Jun 25 15:13:29 2007 Subject: [Histonet] Paraffin specimen clamp In-Reply-To: References: Message-ID: <005401c7b765$423ccfd0$7701a80a@Ford> You need an adjustable jaw clamp (or ?C? clamp) specimen holder. Check with your local Microm Fisher Healthcare rep. Ford M. Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL:? 612-839-1046 Phone:? 763-542-8725 Fax:? 763-546-4830 eMail:? clinicallab@minnesotamedical.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kinsley, David Sent: Monday, June 25, 2007 2:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin specimen clamp Hi, I cut paraffin sections on a Microm HM 355 microtome. I recently received some paraffin samples from Europe and they are rectangular paraffin blocks measuring approximately 32x22x22mm without any plastic cassette attached to them. Does anyone know where I may be able to purchase a different type of specimen holder/clamp that would hold the paraffin block directly without the need for a cassette? Unfortunately, I cannot melt the blocks and re-embed them with cassettes. thanks in advance Dave ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> flhosp.org Mon Jun 25 15:26:32 2007 From: Janet.Bonner <@t> flhosp.org (Bonner, Janet) Date: Mon Jun 25 15:26:46 2007 Subject: [Histonet] Paraffin specimen clamp References: Message-ID: <5F31F38C96781A4FBE3196EBC22D47802E101D@fhosxchmb006.ADVENTISTCORP.NET> David, We occasionally get some of these blocks, often from South America. Though the paraffin is a different media, we melt them, let them soak/infiltrate for about two hours in our paraffin, and reembed them with a cassette. - But you cannot do that. In this instance, I would embed the entire block as you would a piece of tissue, without melting the existing paraffin, and attach a cassette. Chill, cut, and good luck! -Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Kinsley, David Sent: Mon 6/25/2007 3:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin specimen clamp Hi, I cut paraffin sections on a Microm HM 355 microtome. I recently received some paraffin samples from Europe and they are rectangular paraffin blocks measuring approximately 32x22x22mm without any plastic cassette attached to them. Does anyone know where I may be able to purchase a different type of specimen holder/clamp that would hold the paraffin block directly without the need for a cassette? Unfortunately, I cannot melt the blocks and re-embed them with cassettes. thanks in advance Dave ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From AGrobe2555 <@t> aol.com Mon Jun 25 15:46:48 2007 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Mon Jun 25 15:46:56 2007 Subject: [Histonet] Paraffin specimen clamp Message-ID: "In this instance, I would embed the entire block as you would a piece of tissue, without melting the existing paraffin, and attach a cassette." David, I have tried this in the past with very little success. The new, hot paraffin does not adhere to the old, cold paraffin enough to keep the blocks affixed to the cassette for any length of time when subjected to the shearing pressure of the blade while sectioning. I have had to melt, and re-embed the samples in order to section the samples. The most straight-forward way would be to find the appropriate clamp (hopefully at an inexpensive price). Just curious, why can't you melt and re-embed? Best of luck, Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital ************************************** See what's free at http://www.aol.com. From cottamb <@t> ohsu.edu Mon Jun 25 16:46:25 2007 From: cottamb <@t> ohsu.edu (Benjamin Cottam) Date: Mon Jun 25 16:46:57 2007 Subject: [Histonet] Cresyl Violet Staining Message-ID: I have been staining rodent and moonkey retina fixed frozen sections with cresyl violet, .5% for some time. Recently the staining has been washing out in the rinsing/dehyration steps. I have tried inceasing the time in the stain, increasing the stain concentration to 1%, and even shortening the time in the wash and dehration. I also tried moving right from CV to 95% EtOH but after a few minutes, the staining has washed out of the retina. Any suggestions on what to do next would be most helpful. Benjamin Cottam Sr. Research Assistant OHSU-CEI From int09018 <@t> alphahunt.com Mon Jun 25 16:48:59 2007 From: int09018 <@t> alphahunt.com (special address) Date: Mon Jun 25 16:49:11 2007 Subject: [Histonet] RE:looking for block files Message-ID: <000601c7b772$a3e272f0$6500a8c0@LHBLION> I use the older fisher block files for filing my paraffin blocks and wonder if anyone has some they no longer use. There are the single drawer kind and the five drawer kind which are made of plastic. Please contact me directly at lhbhcs@comcast.net . LeRoy Brown HT(ASCP) HTL Histology Consultation Services,Inc. 360-966-7300 www.histocs.com From lpwenk <@t> sbcglobal.net Mon Jun 25 17:41:24 2007 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Mon Jun 25 17:41:38 2007 Subject: [Histonet] HT exam In-Reply-To: <467FC012.9070101@tufts.edu> Message-ID: <002501c7b779$f6ac95e0$0202a8c0@HPPav2> The Jan - June 2007 results will be published in the Fall 2007. The highest possible HT and HTL score is 999. However, the average highest score for the last 7 years is 771 for the HT exam. The following are the highest scores, taken from the ASCP webpage that has links to exam results by year. http://www.ascp.org/Certification/ForProgramDirectors/default.aspx 2006 - 767 2005 - 776 2004 - 788 2003 - 779 2002 - 764 2001 - 775 2000 - 752 In case someone wants to know the highest HTL exam scores for the last 7 years (average highest score = 680): 2006 - 681 2005 - 679 2004 - 725 2003 - 649 2002 - 702 2001 - 652 2000 - 675 In case someone was wondering why the HTL exam averages 80 points lower than the HT exam - well, it's that much harder of an exam. My HTL students have 5 additional months of lectures and labs in comparison with my HT students, and 2 additional years of college biology and chemistry classes. And yet my HTL students' exam scores are always lower than my HT students. Consistently, year after year, no matter who the students are, no matter how I change the HTL curriculum. The HTL exam is just that much harder than the HT exam. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Derek Papalegis Sent: Monday, June 25, 2007 9:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT exam Does anybody know the score range of the HT exam? I just found out what my actual score was and I want to see where I fall in that range. Thanks, Derek -- Derek Papalegis HT (ASCP) Histotechnician Division of Laboratory Animal Medicine Tufts University 136 Harrison Avenue Boston, MA 02111 phone: 617 636-2971 fax: 617 636-8354 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Mon Jun 25 17:56:57 2007 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Mon Jun 25 17:57:14 2007 Subject: [Histonet] ASCP certification In-Reply-To: <0E394B648E5284478A6CCB78E5AFDA2703640DC6@hpes1.HealthPartners.int> Message-ID: <002601c7b77c$22f8b1e0$0202a8c0@HPPav2> 2006 was the last year requiring both practical exams and written exams. As of 2007, the HT and HTL exams are written exams only. By the way, in case you haven't heard, the new requirements for HT exam is an associate degree (or 60 semester hours) with a minimum of 12 semester hours of biology and chemistry combined, and 1 year full time experience in histotechnology under a pathologist. For more information, go to: http://www.ascp.org/Certification/CertifyingExaminations/cert_procedures/def ault.aspx The change to the associate degree was announced for over 5 years, and went into effect Jan. 1, 2005. There is no "grandfathering" or alternate route (except for going through a NAACLS HT program, which can be high school diploma through associate degree). If she wants help with learning the theory, or if she needs to go through a NAACLS program due to not having an associate degree, there are some HT NAACLS programs available (for a price) that allow the person to take the NAACLS HT courses through the lab they are working at now, via internet and/or teleconferences. Go to the NAACLS web page: http://naacls.org/search/programs.asp Three programs that I am aware of: Maryland - Harford; Ohio - Columbus State; Indiana - IU. I think one of the Florida schools was setting this up, but I don't know where they are in the process (sorry, Florida). Peggy A. Wenk, HT(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Monday, June 25, 2007 2:27 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] ASCP certification I have 3 histotechs that still need to achieve their ASCP certification. Are there any practice exams out anywhere? One tech has been around for 15 years and is afraid she will not remember her theory enough to pass a current exam. Thanks ahead of time! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From scorpionrider <@t> cox.net Mon Jun 25 18:02:41 2007 From: scorpionrider <@t> cox.net (Mark Turner) Date: Mon Jun 25 18:02:46 2007 Subject: [Histonet] ASCP certification Message-ID: <31362708.1182812561589.JavaMail.root@fed1wml13.mgt.cox.net> What about people who passed the HT practical exam but took the written exam and failed it before the new regulations went into effect? A person in our lab did this and is under the impression that she has 5 years to pass the written test to become certified, even though she doesn't have a degree. If there is no "grandfathering," is she incorrect in her assumption? Mark Turner, HT(ASCP) ---- Lee & Peggy Wenk wrote: > 2006 was the last year requiring both practical exams and written exams. > > As of 2007, the HT and HTL exams are written exams only. > > By the way, in case you haven't heard, the new requirements for HT exam is > an associate degree (or 60 semester hours) with a minimum of 12 semester > hours of biology and chemistry combined, and 1 year full time experience in > histotechnology under a pathologist. > > For more information, go to: > http://www.ascp.org/Certification/CertifyingExaminations/cert_procedures/def > ault.aspx > > The change to the associate degree was announced for over 5 years, and went > into effect Jan. 1, 2005. There is no "grandfathering" or alternate route > (except for going through a NAACLS HT program, which can be high school > diploma through associate degree). > > If she wants help with learning the theory, or if she needs to go through a > NAACLS program due to not having an associate degree, there are some HT > NAACLS programs available (for a price) that allow the person to take the > NAACLS HT courses through the lab they are working at now, via internet > and/or teleconferences. > > Go to the NAACLS web page: > http://naacls.org/search/programs.asp > > Three programs that I am aware of: Maryland - Harford; Ohio - Columbus > State; Indiana - IU. I think one of the Florida schools was setting this up, > but I don't know where they are in the process (sorry, Florida). > > Peggy A. Wenk, HT(ASCP)SLS > William Beaumont Hospital > Royal Oak, MI 48073 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, > Dorothy L > Sent: Monday, June 25, 2007 2:27 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] ASCP certification > > I have 3 histotechs that still need to achieve their ASCP certification. > Are there any practice exams out anywhere? One tech has been around for > 15 years and is afraid she will not remember her theory enough to pass a > current exam. Thanks ahead of time! > > Dorothy Webb, HT (ASCP) > Histology Technical Supervisor > Regions Hospital, Pathology Department > 640 Jackson Street, Saint Paul, MN 55101-2595 > Phone: 651-254-2962 > Fax: 651-254-2741 > Regions Hospital is part of the HealthPartners family of care > ________________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they are > addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, please be > advised that you have received this e-mail in error and that any use, > dissemination, forwarding, printing, or copying of this e-mail is strictly > prohibited. > > If you have received this e-mail in error, please immediately notify the > HealthPartners Support Center by telephone at (952) 967-6600. You will be > reimbursed for reasonable costs incurred in notifying us. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jason.Wiese <@t> va.gov Mon Jun 25 18:08:09 2007 From: Jason.Wiese <@t> va.gov (Wiese, Jason VHAROS) Date: Mon Jun 25 18:08:21 2007 Subject: [Histonet] ASCP certification In-Reply-To: <31362708.1182812561589.JavaMail.root@fed1wml13.mgt.cox.net> References: <31362708.1182812561589.JavaMail.root@fed1wml13.mgt.cox.net> Message-ID: <70EEF3D43B3C164C94037D811B2BE193E12AEA@VHAV20MSGA3.v20.med.va.gov> If she received a letter that said "... because you had all your paperwork in before such-n-such date, then you have five years to complete the test..." then she has five years to complete the test. I have seen it a few times, and yes... if they give you five years, then you get five years. However, if she is making an assumption without the letter, she might be mistaken. When she got her results back, it probably came with such a letter, and it wouldn't be the first I saw. Jason -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Turner Sent: Monday, June 25, 2007 4:03 PM To: 'Webb, Dorothy L'; lpwenk@sbcglobal.net; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASCP certification What about people who passed the HT practical exam but took the written exam and failed it before the new regulations went into effect? A person in our lab did this and is under the impression that she has 5 years to pass the written test to become certified, even though she doesn't have a degree. If there is no "grandfathering," is she incorrect in her assumption? Mark Turner, HT(ASCP) ---- Lee & Peggy Wenk wrote: > 2006 was the last year requiring both practical exams and written exams. > > As of 2007, the HT and HTL exams are written exams only. > > By the way, in case you haven't heard, the new requirements for HT exam is > an associate degree (or 60 semester hours) with a minimum of 12 semester > hours of biology and chemistry combined, and 1 year full time experience in > histotechnology under a pathologist. > > For more information, go to: > http://www.ascp.org/Certification/CertifyingExaminations/cert_procedures /def > ault.aspx > > The change to the associate degree was announced for over 5 years, and went > into effect Jan. 1, 2005. There is no "grandfathering" or alternate route > (except for going through a NAACLS HT program, which can be high school > diploma through associate degree). > > If she wants help with learning the theory, or if she needs to go through a > NAACLS program due to not having an associate degree, there are some HT > NAACLS programs available (for a price) that allow the person to take the > NAACLS HT courses through the lab they are working at now, via internet > and/or teleconferences. > > Go to the NAACLS web page: > http://naacls.org/search/programs.asp > > Three programs that I am aware of: Maryland - Harford; Ohio - Columbus > State; Indiana - IU. I think one of the Florida schools was setting this up, > but I don't know where they are in the process (sorry, Florida). > > Peggy A. Wenk, HT(ASCP)SLS > William Beaumont Hospital > Royal Oak, MI 48073 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, > Dorothy L > Sent: Monday, June 25, 2007 2:27 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] ASCP certification > > I have 3 histotechs that still need to achieve their ASCP certification. > Are there any practice exams out anywhere? One tech has been around for > 15 years and is afraid she will not remember her theory enough to pass a > current exam. Thanks ahead of time! > > Dorothy Webb, HT (ASCP) > Histology Technical Supervisor > Regions Hospital, Pathology Department > 640 Jackson Street, Saint Paul, MN 55101-2595 > Phone: 651-254-2962 > Fax: 651-254-2741 > Regions Hospital is part of the HealthPartners family of care > ________________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they are > addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, please be > advised that you have received this e-mail in error and that any use, > dissemination, forwarding, printing, or copying of this e-mail is strictly > prohibited. > > If you have received this e-mail in error, please immediately notify the > HealthPartners Support Center by telephone at (952) 967-6600. You will be > reimbursed for reasonable costs incurred in notifying us. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Mon Jun 25 18:13:42 2007 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Mon Jun 25 18:13:56 2007 Subject: [Histonet] Cresyl Violet Staining In-Reply-To: Message-ID: <002701c7b77e$79812ea0$0202a8c0@HPPav2> Wondering if the dye should be cresyl echt violet, now known as cresyl violet acetate, rather than cresyl violet. This is what we use to demonstrate Nissl. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Benjamin Cottam Sent: Monday, June 25, 2007 5:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cresyl Violet Staining I have been staining rodent and moonkey retina fixed frozen sections with cresyl violet, .5% for some time. Recently the staining has been washing out in the rinsing/dehyration steps. I have tried inceasing the time in the stain, increasing the stain concentration to 1%, and even shortening the time in the wash and dehration. I also tried moving right from CV to 95% EtOH but after a few minutes, the staining has washed out of the retina. Any suggestions on what to do next would be most helpful. Benjamin Cottam Sr. Research Assistant OHSU-CEI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From m.kap.1 <@t> erasmusmc.nl Tue Jun 26 03:48:52 2007 From: m.kap.1 <@t> erasmusmc.nl (M. Kap) Date: Tue Jun 26 03:49:13 2007 Subject: [Histonet] fully automated tissue embedding Message-ID: <1275.10.66.2.46.1182847732.squirrel@webmail.erasmusmc.nl> Dear all, Within the next couple of years we are planning to invest in a fully automated tissue embedding machine. As far as I know there is no such device in operation here in Holland. So it's hard to get by any usefull tips. Can anyone of you send me your experiences and tips&tricks? What are the pitfalls, what are the benefits, how must we adapt our routine to make it work et cetera. Many thanks, Marcel Kap Pathology dept Erasmus University Hospital Rotterdam, The Netherlands From lpwenk <@t> sbcglobal.net Tue Jun 26 04:44:24 2007 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Tue Jun 26 04:44:46 2007 Subject: [Histonet] ASCP certification In-Reply-To: <31362708.1182812561589.JavaMail.root@fed1wml13.mgt.cox.net> Message-ID: <000c01c7b7d6$95a01850$0202a8c0@HPPav2> Yes, the people who took the HT exam before the associate degree deadline still have a total of 5 tries in 3 years, according to the ASCP Exam booklet http://www.ascp.org/Certification/pdf/booklet.pdf If they do not take all the 5 tries in the 3 year period (for example, tried it just 3 times in the 3 year period, and still has 2 tries left), then since they are under a new 3 year time period, the last 2 tries are under the new routes, i.e., the associate degree or NAACLS graduate. Since the last year that people could take the exam under this ruling was Dec. 31, 2004, they have until Dec. 2007 to complete their remaining tries under the "old" route of high school diploma + 2 years OJT. If the high school route person took their HT exam for the first time in, say, May 2004, then the 3 year time period has elapsed (May 2007), and they are now under the new routes (associate degree or NAACLS graduate only). I apologize for not clarifying my previous email, that it was for NEW applicants to take the HT exam. They do not have a grandfather clause for taking the exam with X number of years experience but no associate degree/NAACLS graduate. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 From: Mark Turner [mailto:scorpionrider@cox.net] Sent: Monday, June 25, 2007 7:03 PM To: 'Webb, Dorothy L'; lpwenk@sbcglobal.net; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASCP certification What about people who passed the HT practical exam but took the written exam and failed it before the new regulations went into effect? A person in our lab did this and is under the impression that she has 5 years to pass the written test to become certified, even though she doesn't have a degree. If there is no "grandfathering," is she incorrect in her assumption? Mark Turner, HT(ASCP) ---- Lee & Peggy Wenk wrote: > 2006 was the last year requiring both practical exams and written exams. > > As of 2007, the HT and HTL exams are written exams only. > > By the way, in case you haven't heard, the new requirements for HT exam is > an associate degree (or 60 semester hours) with a minimum of 12 semester > hours of biology and chemistry combined, and 1 year full time experience in > histotechnology under a pathologist. > > For more information, go to: > http://www.ascp.org/Certification/CertifyingExaminations/cert_procedures/def > ault.aspx > > The change to the associate degree was announced for over 5 years, and went > into effect Jan. 1, 2005. There is no "grandfathering" or alternate route > (except for going through a NAACLS HT program, which can be high school > diploma through associate degree). > > If she wants help with learning the theory, or if she needs to go through a > NAACLS program due to not having an associate degree, there are some HT > NAACLS programs available (for a price) that allow the person to take the > NAACLS HT courses through the lab they are working at now, via internet > and/or teleconferences. > > Go to the NAACLS web page: > http://naacls.org/search/programs.asp > > Three programs that I am aware of: Maryland - Harford; Ohio - Columbus > State; Indiana - IU. I think one of the Florida schools was setting this up, > but I don't know where they are in the process (sorry, Florida). > > Peggy A. Wenk, HT(ASCP)SLS > William Beaumont Hospital > Royal Oak, MI 48073 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, > Dorothy L > Sent: Monday, June 25, 2007 2:27 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] ASCP certification > > I have 3 histotechs that still need to achieve their ASCP certification. > Are there any practice exams out anywhere? One tech has been around for > 15 years and is afraid she will not remember her theory enough to pass a > current exam. Thanks ahead of time! > > Dorothy Webb, HT (ASCP) > Histology Technical Supervisor > Regions Hospital, Pathology Department > 640 Jackson Street, Saint Paul, MN 55101-2595 > Phone: 651-254-2962 > Fax: 651-254-2741 > Regions Hospital is part of the HealthPartners family of care > ________________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they are > addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, please be > advised that you have received this e-mail in error and that any use, > dissemination, forwarding, printing, or copying of this e-mail is strictly > prohibited. > > If you have received this e-mail in error, please immediately notify the > HealthPartners Support Center by telephone at (952) 967-6600. You will be > reimbursed for reasonable costs incurred in notifying us. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From susanbachus <@t> verizon.net Tue Jun 26 09:45:49 2007 From: susanbachus <@t> verizon.net (Susan Bachus) Date: Tue Jun 26 09:46:13 2007 Subject: [Histonet] Cresyl Violet Staining References: Message-ID: <000f01c7b800$b06e7c10$2f01a8c0@RESLAPTOP> The pH can be critical for cresyl violet to work--check your protocol to see what pH is recommended for this application. Also, the stain will gradually be depleted from the solution after long use, so sometimes making up a fresh batch can improve your results. Good luck! Susan ----- Original Message ----- From: "Benjamin Cottam" To: Sent: Monday, June 25, 2007 5:46 PM Subject: [Histonet] Cresyl Violet Staining >I have been staining rodent and moonkey retina fixed frozen sections > with cresyl violet, .5% for some time. Recently the staining has been > washing out in the rinsing/dehyration steps. I have tried inceasing the > time in the stain, increasing the stain concentration to 1%, and even > shortening the time in the wash and dehration. I also tried moving > right from CV to 95% EtOH but after a few minutes, the staining has > washed out of the retina. Any suggestions on what to do next would be > most helpful. > Benjamin Cottam > Sr. Research Assistant > OHSU-CEI > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jessgrocki <@t> yahoo.com Tue Jun 26 11:06:15 2007 From: jessgrocki <@t> yahoo.com (Jessica Piche) Date: Tue Jun 26 11:06:29 2007 Subject: [Histonet] Renal Cell Carcinoma Marker Message-ID: <650175.93548.qm@web82010.mail.mud.yahoo.com> Hello, I was wondering if anyone knows of a place to get Renal Cell Carcinoma marker? And also if there is an in vitro diagnostic version of it instead of research only? We got our last batch from NovoCastra. Thanks, Jessica Piche-Grocki, HT(ASCP) From kristinsavage <@t> hotmail.com Tue Jun 26 11:07:40 2007 From: kristinsavage <@t> hotmail.com (Kristin Savage) Date: Tue Jun 26 11:07:51 2007 Subject: [Histonet] (no subject) Message-ID: Hi- my name is Kristin, a histotech out of Bozeman MT and I'm new to Histonet... just wanted to put the word out there about a full-time opening in Kalispell, MT, one of the most gorgeous places on earth right next to Glacier Nat'l park. It's for a solo-tech position for a dermpathologist needing help this mid-end of August. I'm setting up a lab for him this summer and he's asked me to help find someone! Serious inquiries can write to kristinsavage@hotmail.com and leave ph.# Thanks everyone! Kristin Savage HT(ASCP)cm Bozeman, MT 59714 _________________________________________________________________ Don’t miss your chance to WIN $10,000 and other great prizes from Microsoft Office Live http://clk.atdmt.com/MRT/go/aub0540003042mrt/direct/01/ From Alexander.Cummins2 <@t> verizon.net Tue Jun 26 11:28:28 2007 From: Alexander.Cummins2 <@t> verizon.net (Alex Cummins) Date: Tue Jun 26 11:28:27 2007 Subject: [Histonet] HRP Substrates In-Reply-To: <0JK900A973MTCEZ0@vms169133.mailsrvcs.net> References: <0JK900A973MTCEZ0@vms169133.mailsrvcs.net> Message-ID: Hi all. I am looking for a peroxidase substrate that will withstand paraffin embedding. Something other that DAB. AEC and TMB are not stable thru embedding if memory serves me right. Any suggestions? Thanks ahead of time Alex From scoop <@t> mail.nih.gov Tue Jun 26 11:37:08 2007 From: scoop <@t> mail.nih.gov (Sharon Cooperman) Date: Tue Jun 26 11:37:17 2007 Subject: [Histonet] what percentage of cells in bone marrow are erythrocytes Message-ID: Dear All, I know this may be an unanswerable question but does anyone know what percentage of the cells in bone marrow are mature erythrocytes and what percentage are erythroblasts? (in normal marrow) I'm really interested in mouse bone marrow, but if anyone knows the numbers for human or any other species that would at least help me to guesstimate the numbers for mouse. Thanks in advance for any help. Sharon -- Sharon Cooperman NIH, NICHD, CBMB 301.435-8417 Building 18T, room 101 301.402-0078 fax Bethesda, MD 20892 From jfish <@t> gladstone.ucsf.edu Tue Jun 26 11:49:37 2007 From: jfish <@t> gladstone.ucsf.edu (Jo Dee Fish) Date: Tue Jun 26 11:50:13 2007 Subject: [Histonet] Paraffin specimen clamp In-Reply-To: Message-ID: <000601c7b811$fb51def0$2e0d010a@JFISH> Sorry, I don't remember who sent the original email. This can work. You need to let the old paraffin sit in the new, hot paraffin for just a few minutes (3 or 4) before beginning the cooling down. The old paraffin will melt along the outside of the block and it helps it to "blend" with the new paraffin. I do this all of the time to embed small mouse embryos, e.g. 9.5dpc, so that I can maintain orientation without preembedding in agar. Works great for me! Good luck, Jo Dee Jo Dee Fish Research Technologist III Gladstone Institute of Cardiovascular Disease Telephone: (415) 734-2567 Fax: (415) 355-0824 E-mail: jfish@gladstone.ucsf.edu Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of AGrobe2555@aol.com Sent: Monday, June 25, 2007 1:47 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Paraffin specimen clamp "In this instance, I would embed the entire block as you would a piece of tissue, without melting the existing paraffin, and attach a cassette." David, I have tried this in the past with very little success. The new, hot paraffin does not adhere to the old, cold paraffin enough to keep the blocks affixed to the cassette for any length of time when subjected to the shearing pressure of the blade while sectioning. I have had to melt, and re-embed the samples in order to section the samples. The most straight-forward way would be to find the appropriate clamp (hopefully at an inexpensive price). Just curious, why can't you melt and re-embed? Best of luck, Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital ************************************** See what's free at http://www.aol.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Tue Jun 26 12:09:26 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Jun 26 12:09:34 2007 Subject: [Histonet] HRP Substrates In-Reply-To: References: <0JK900A973MTCEZ0@vms169133.mailsrvcs.net> Message-ID: <6.0.0.22.1.20070626110704.01b4adc0@gemini.msu.montana.edu> What about NOVA Red from Vector? It withstands alcohols, but how much I am not certain of. You can contact Craig Pow at VECTOR email is: cspow@vectorlabs.com He is tech services and very helpful At 10:28 AM 6/26/2007, you wrote: >Hi all. I am looking for a peroxidase substrate that will withstand paraffin >embedding. Something other that DAB. AEC and TMB are not stable thru >embedding if memory serves me right. Any suggestions? Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From gcallis <@t> montana.edu Tue Jun 26 12:16:40 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Jun 26 12:16:46 2007 Subject: [Histonet] A publication to help with rodent CD31 dilemma, in particular rat CD31(PECAM) on decalcified bone Message-ID: <6.0.0.22.1.20070626111018.01b20508@gemini.msu.montana.edu> A PUBMED search led to a wonderful publication titled An evaluation of the immunohistochemistry benefits of boric acid antigen retreival on rat decalcified joint. Wilson, Elaine et al. J Immunological Methods V. 322, Issue 1-2, Pages 137-142, 2007. This had excellent information on fixation, low temperature retrievals, and also decalcification of bone to see CD31 and PCNA immunohistochemistry. Since CD31 is in Histonet inquiries so much recently, it may be a help to those trying to stain for this marker. If anyone needs the pdf, I will send privately. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From c.m.vanderloos <@t> amc.uva.nl Tue Jun 26 12:17:04 2007 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Tue Jun 26 12:17:21 2007 Subject: [Histonet] RE: HRP Substrates Message-ID: <6daad26d5389.6d53896daad2@amc.uva.nl> Alex, Try Vector NovaRed or Vector VIP. At least those reaction products are not dissolving in organic mounting media, nicely fitting your paraffin embedding plan. If this doesn't work go to beta-GAL visualization using X-gal instead. That turquoise reaction product withstands everything! Cheers, Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Tue, 26 Jun 2007 12:28:28 -0400 From: "Alex Cummins" Subject: [Histonet] HRP Substrates To: Hi all. I am looking for a peroxidase substrate that will withstand paraffin embedding. Something other that DAB. AEC and TMB are not stable thru embedding if memory serves me right. Any suggestions? Thanks ahead of time Alex From meeva <@t> med.unc.edu Tue Jun 26 12:26:06 2007 From: meeva <@t> med.unc.edu (Mervi Eeva) Date: Tue Jun 26 12:26:30 2007 Subject: [Histonet] p-MEK 1&2 and IRF-7 Message-ID: <46814C2E.1010108@med.unc.edu> Hi, Does anyone have IHC experience using p-MEK 1&2 and IRF-7 antibodies on FFPE human tissue ? I'd appreciate any comments and tips since I have problems with these antibodies. Thanks, Mervi/ UNC From jcline <@t> wchsys.org Tue Jun 26 12:28:38 2007 From: jcline <@t> wchsys.org (Joyce Cline) Date: Tue Jun 26 12:28:49 2007 Subject: [Histonet] slide racks available Message-ID: <000001c7b817$6fc68bf0$1d2a14ac@wchsys.org> Hello Everyone, I have six stainless racks for the old Shandon Varistainer that was two levels and looked like the old autotechnicons. (Does that tell how long I have been doing this?) The racks are triangle shaped. Contact me at jcline@wchsys.org. I also have a brand new foot pedal for a Shandon embedding center. The foot pedal has "Treadlite" cat. No T-51-SC36 on it. Cleaning out drawers, you all know how it goes. ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From Eric.C.Kellar <@t> questdiagnostics.com Tue Jun 26 12:54:01 2007 From: Eric.C.Kellar <@t> questdiagnostics.com (Kellar, Eric C) Date: Tue Jun 26 12:54:23 2007 Subject: [Histonet] what percentage of cells in bone marrow are erythrocytes Message-ID: <6843061CE6B98E4B96590D4F299618F801583C2F@qdcws0117.us.qdx.com> Cell content of mouse bone marrow: Blasts <1% Promyelocytes, myelocytes 10% Metamyelocytes, band neutrophils, segs 24% Eosinophils 3% Monocytes-macrophage 10% Erythroblasts 20% Lymphocytes 32% Megakaryocytes <1% Approximately 99% of cells in the marrow are recognizable precursors of red blood cells, granulocytes, monocytes and B lymphocytes. Other cells in the marrow are blast cells ~1%, which ultimately give rise to the morphologically recognizable precursors. The whole marrow of mice contains ~16x10(9) cells per kg body weight, similar to that of humans. Reticulocytes are released from bone marrow after 2 to 3 days and circulate for an additional 1 or 2 days before maturing into an erythrocyte. Marrow erythron content - * rubriblasts = 1% or less * prorubricytes = 1 to 4% * rubricytes = 10 to 20% * metarubricytes = 5 to 10% About 1% of the circulating erythrocyte mass is generated by the marrow each day so that on a given day there should be 1% reticulocytes in the peripheral blood. These replace the 1% of the erythrocyte mass that dies of old age each day. N:C ratio decreases. The rubriblast and prorubricyte have an N:C ratio of 4:1; the rubricyte and metarubricyte N:C ratio is 1:1. Hope this helps! Eric C. Kellar Quest Diagnostics Histology Laboratory Supervisor South Florida -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon Cooperman Sent: Tuesday, June 26, 2007 12:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] what percentage of cells in bone marrow are erythrocytes Dear All, I know this may be an unanswerable question but does anyone know what percentage of the cells in bone marrow are mature erythrocytes and what percentage are erythroblasts? (in normal marrow) I'm really interested in mouse bone marrow, but if anyone knows the numbers for human or any other species that would at least help me to guesstimate the numbers for mouse. Thanks in advance for any help. Sharon -- Sharon Cooperman NIH, NICHD, CBMB 301.435-8417 Building 18T, room 101 301.402-0078 fax Bethesda, MD 20892 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ============================================================================== The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. ============================================================================== From danarobinmarshal <@t> aol.com Tue Jun 26 12:58:22 2007 From: danarobinmarshal <@t> aol.com (danarobinmarshal@aol.com) Date: Tue Jun 26 12:58:44 2007 Subject: [Histonet] positive control marker for human epithelial cells Message-ID: <8C98632B8B877D6-1E90-521A@WEBMAIL-MA17.sysops.aol.com> Hi everyone, What are you using for a positive staining molecule for human epithelial cell IHC.? I am really looking to check the integrity of some old FFPE blocks for IHC before I try my previously untested antibody from abcam, where I will be looking for a less abundant protein.? I presume that a keratin will do but I don't know which one to pick. Thanks, Dana Marshall Meharry Medical College Nashville, TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From Jackie.O'Connor <@t> abbott.com Tue Jun 26 13:03:06 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Tue Jun 26 13:03:44 2007 Subject: [Histonet] Slide storage index card dividers In-Reply-To: <6daad26d5389.6d53896daad2@amc.uva.nl> Message-ID: I know someone knows a couple of vendors where I can get colored pre-cut slide dividers. I've been racking my brain all morning. Someone please rest my brain. Jackie From dmccaig <@t> ckha.on.ca Tue Jun 26 13:07:13 2007 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Tue Jun 26 13:07:26 2007 Subject: [Histonet] Fast green/light green counterstain Message-ID: Does anyone have a version of fast/light green that could be used for PAS for Fungi. We want to program it on the autostainer and using the traditional light green that requires dilution before use does not work. We are looking for a counterstain that can be loaded when the slides are loaded. Diana From HornHV <@t> archildrens.org Tue Jun 26 13:17:20 2007 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Tue Jun 26 13:17:38 2007 Subject: [Histonet] Slide storage index card dividers In-Reply-To: References: <6daad26d5389.6d53896daad2@amc.uva.nl> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB71FB@EMAIL.archildrens.org> Lab Storage Systems. www.labstore.com Ours are yellow and cat number is SIM-1001 but they come in several other colors are well. Very nice cardboard markers. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Tuesday, June 26, 2007 1:03 PM To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] Slide storage index card dividers I know someone knows a couple of vendors where I can get colored pre-cut slide dividers. I've been racking my brain all morning. Someone please rest my brain. Jackie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From NMargaryan <@t> childrensmemorial.org Tue Jun 26 14:10:30 2007 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Tue Jun 26 14:10:10 2007 Subject: [Histonet] (no subject) Message-ID: Hello Dear Friends, I am looking for very good and stabile Mounting Media on Aqua based. Any suggestion is very appreciated, Naira Naira V. Margaryan, D.V.M., Ph.D. Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3394 Tel: 773-755-6570/ext-8 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 From asmith <@t> mail.barry.edu Tue Jun 26 14:21:33 2007 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Tue Jun 26 14:21:46 2007 Subject: [Histonet] HRP Substrates In-Reply-To: <6.0.0.22.1.20070626110704.01b4adc0@gemini.msu.montana.edu> Message-ID: <7DBCCC1FBC77C94F99F920D0CA6400B61E40B3@exchsrv01.barrynet.barry.edu> Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Tuesday, June 26, 2007 1:09 PM To: Alex Cummins; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] HRP Substrates What about NOVA Red from Vector? It withstands alcohols, but how much I am not certain of. You can contact Craig Pow at VECTOR email is: cspow@vectorlabs.com He is tech services and very helpful At 10:28 AM 6/26/2007, you wrote: >Hi all. I am looking for a peroxidase substrate that will withstand paraffin >embedding. Something other that DAB. AEC and TMB are not stable thru >embedding if memory serves me right. Any suggestions? Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From asmith <@t> mail.barry.edu Tue Jun 26 14:26:24 2007 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Tue Jun 26 14:26:37 2007 Subject: [Histonet] HRP Substrates In-Reply-To: <6.0.0.22.1.20070626110704.01b4adc0@gemini.msu.montana.edu> Message-ID: <7DBCCC1FBC77C94F99F920D0CA6400B61E40B4@exchsrv01.barrynet.barry.edu> I have found that Nova Red (which is really rust-colored) resists both alcohol and warm (45 degrees C) xylene. I have not tried hot xylene. Vector's VIP (very intense purple) also resists alcohol and xylene. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Tuesday, June 26, 2007 1:09 PM To: Alex Cummins; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] HRP Substrates What about NOVA Red from Vector? It withstands alcohols, but how much I am not certain of. You can contact Craig Pow at VECTOR email is: cspow@vectorlabs.com He is tech services and very helpful At 10:28 AM 6/26/2007, you wrote: >Hi all. I am looking for a peroxidase substrate that will withstand paraffin >embedding. Something other that DAB. AEC and TMB are not stable thru >embedding if memory serves me right. Any suggestions? Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From SCOTT.TURNER <@t> SPCORP.COM Tue Jun 26 14:37:04 2007 From: SCOTT.TURNER <@t> SPCORP.COM (Turner, Scott) Date: Tue Jun 26 14:37:16 2007 Subject: [Histonet] HRP Substrates In-Reply-To: <7DBCCC1FBC77C94F99F920D0CA6400B61E40B4@exchsrv01.barrynet.barry.edu> Message-ID: <9A919A5D70313A4D9C56A025710874082907E3@kenmsg40.us.schp.com> While we're on the subject of HRP substrates, does anyone know of an HRP substrate that will fluoresce? I know that some Alk-Phos substrates like Vector's AP Red do, but I'm looking for an HRP that will work for a particular IF assay we're working on. Scott Turner Schering-Plough Biopharma Palo Alto, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith, Allen Sent: Tuesday, June 26, 2007 12:26 PM To: Gayle Callis Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HRP Substrates I have found that Nova Red (which is really rust-colored) resists both alcohol and warm (45 degrees C) xylene. I have not tried hot xylene. Vector's VIP (very intense purple) also resists alcohol and xylene. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Tuesday, June 26, 2007 1:09 PM To: Alex Cummins; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] HRP Substrates What about NOVA Red from Vector? It withstands alcohols, but how much I am not certain of. You can contact Craig Pow at VECTOR email is: cspow@vectorlabs.com He is tech services and very helpful At 10:28 AM 6/26/2007, you wrote: >Hi all. I am looking for a peroxidase substrate that will withstand paraffin >embedding. Something other that DAB. AEC and TMB are not stable thru >embedding if memory serves me right. Any suggestions? Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From GDawson <@t> dynacaremilwaukee.com Tue Jun 26 14:56:06 2007 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Tue Jun 26 14:56:18 2007 Subject: [Histonet] Renal Cell Carcinoma Marker In-Reply-To: <650175.93548.qm@web82010.mail.mud.yahoo.com> Message-ID: Jessica, I use the Renal Cell Carcinoma marker from Cell Marque (Cat. #CMC846) with great success. It is an IVD antibody and it's clone is PN-15. Good Luck, Glen Dawson IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jessica Piche Sent: Tuesday, June 26, 2007 11:06 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Renal Cell Carcinoma Marker Hello, I was wondering if anyone knows of a place to get Renal Cell Carcinoma marker? And also if there is an in vitro diagnostic version of it instead of research only? We got our last batch from NovoCastra. Thanks, Jessica Piche-Grocki, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Tue Jun 26 15:03:44 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Jun 26 15:03:56 2007 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: <6.0.0.22.1.20070626140304.01b19148@gemini.msu.montana.edu> What application, for solvent sensitive chromogens or immunofluorescence? At 01:10 PM 6/26/2007, you wrote: >Hello Dear Friends, > > > >I am looking for very good and stabile Mounting Media on Aqua based. > > > >Any suggestion is very appreciated, > >Naira > >Naira V. Margaryan, D.V.M., Ph.D. > >Children's Memorial Research Center > >2300 Children's Plaza, Box 222 > >Chicago, IL 60614-3394 > >Tel: 773-755-6570/ext-8 > >Fax: 773-755-6594 > >nmargaryan@childrensmemorial.org > > > > >For Express Mail: > >CMRC, Room C.473 > >2430 N. Halsted Street > >Chicago, IL 60614-4314 > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From gcallis <@t> montana.edu Tue Jun 26 15:21:21 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Jun 26 15:21:30 2007 Subject: fluorescing RE: [Histonet] HRP Substrates In-Reply-To: <9A919A5D70313A4D9C56A025710874082907E3@kenmsg40.us.schp.co m> References: <7DBCCC1FBC77C94F99F920D0CA6400B61E40B4@exchsrv01.barrynet.barry.edu> <9A919A5D70313A4D9C56A025710874082907E3@kenmsg40.us.schp.com> Message-ID: <6.0.0.22.1.20070626141140.01b58ea8@gemini.msu.montana.edu> Scott, DAB will fluoresce when excited at same wavelength as FITC. You can discuss details of this with John Kiernan who just wrote a wonderful review of myelin staining protocols for the Journal of Histotechnology special neuropathology edition --- just received this week. John discusses this in his review. Also, if you have the CRi Nuance spectral imaging device, you can make any chromogen appear as fluorescence, even as a single, double or triple staining and also get rid of any autofluorescence. Check out their website for details. At 01:37 PM 6/26/2007, you wrote: >While we're on the subject of HRP substrates, does anyone know of an HRP >substrate that will fluoresce? I know that some Alk-Phos substrates >like Vector's AP Red do, but I'm looking for an HRP that will work for a >particular IF assay we're working on. > >Scott Turner >Schering-Plough Biopharma >Palo Alto, CA > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith, >Allen >Sent: Tuesday, June 26, 2007 12:26 PM >To: Gayle Callis >Cc: histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] HRP Substrates > >I have found that Nova Red (which is really rust-colored) resists both >alcohol and warm (45 degrees C) xylene. I have not tried hot xylene. >Vector's VIP (very intense purple) also resists alcohol and xylene. > >Allen A. Smith, Ph.D. >Professor of Anatomy >Barry University School of Graduate Medical Sciences > Podiatric Medicine and Surgery >Miami Shores, Florida 33161 > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle >Callis >Sent: Tuesday, June 26, 2007 1:09 PM >To: Alex Cummins; Histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] HRP Substrates > >What about NOVA Red from Vector? It withstands alcohols, but how much I >am not certain of. You can contact Craig Pow at VECTOR email >is: cspow@vectorlabs.com > >He is tech services and very helpful > > > >At 10:28 AM 6/26/2007, you wrote: > >Hi all. I am looking for a peroxidase substrate that will withstand >paraffin > >embedding. Something other that DAB. AEC and TMB are not stable thru > >embedding if memory serves me right. Any suggestions? > >Gayle Callis >MT,HT,HTL(ASCP) >Research Histopathology Supervisor >Veterinary Molecular Biology >Montana State University - Bozeman >PO Box 173610 >Bozeman MT 59717-3610 > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >The information transmitted is intended only for the person or entity to >which it is addressed and may contain confidential, and/or privileged >material. No confidentiality or privilege is waived or lost by any >errant transmission. If you receive this message in error, please >immediately delete it and all copies of it from your system and notify >the sender. E-mail transmission cannot be guaranteed to be secure or >error-free as information could be intercepted, corrupted, lost, >destroyed, arrive late or incomplete, or contain viruses. >Barry University - Miami Shores, FL (http://www.barry.edu) > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >********************************************************************* >This message and any attachments are solely for the >intended recipient. If you are not the intended recipient, >disclosure, copying, use or distribution of the information >included in this message is prohibited -- Please >immediately and permanently delete. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From AGrobe2555 <@t> aol.com Tue Jun 26 15:49:31 2007 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Tue Jun 26 15:49:42 2007 Subject: [Histonet] Re: Fluorescent DAB????? Message-ID: Gayle Callis wrote: "DAB will fluoresce when excited at same wavelength as FITC." My questions are: Assuming that all the parameters were kept equivalent within a "batch" of slides: Can it be used in image analysis to quantify expression of a particular epitope of interest among different samples in a batch of stained slides? Or is DAB precipitate more of a random quantity depending on substrate availability/enzyme activity and therefore cannot be used to compare among slides (I realize that enzyme kinetics make the word "random" a bit of an contradiction in this sense)? This is based more upon the premise that the fluorescent secondary only has so many places to bind vs. HRP that creates more color the longer it works......(at least until the reaction is stopped or the substrate/cofactors are exhausted). Thanks, Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital ************************************** See what's free at http://www.aol.com. From rgarhart <@t> system1.net Tue Jun 26 15:49:56 2007 From: rgarhart <@t> system1.net (Robert Garhart) Date: Tue Jun 26 15:50:40 2007 Subject: [Histonet] Histotech and Lab Supervisor Opportunities In-Reply-To: <6.2.5.6.2.20070413112008.01faaaa8@vet.upenn.edu> References: <6.2.5.6.2.20070413112008.01faaaa8@vet.upenn.edu> Message-ID: I am a recruiter in Laboratory Diagnostics with a client who is looking for either histotechs or histotechnologists (also some Lab Supervisor Positions) in the following states. You must meet all state and local licensing requirements as well as CLIA requirements. FL PA NJ NY CT TX OH Lab Supervisor Positions potentially available in the same locations. Relo assist with supervisor positions. Call to further discuss and indicate that you are responding to Histonet. Robert Garhart Executive Recruiter System 1 Search 864.627.0012 Phone 864.627.0013 Fax 866.797.8361 Toll Free rgarhart@system1.net Website: www.system1.net From gcallis <@t> montana.edu Tue Jun 26 16:29:11 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Jun 26 16:29:22 2007 Subject: [Histonet] Re: Fluorescent DAB????? In-Reply-To: References: Message-ID: <6.0.0.22.1.20070626152129.01b31ec0@gemini.msu.montana.edu> I can't answer your question - take it up with John Kiernan per my email to Histonet. This was used as a method to visualize myelinated nerves and fibers - John has more experience with the chemistry and use of this chromogen's fluorescence, and he did send at abstract to me for a European Neuroscience Association meeting in 2000. The subject should certainly generate some interesting discussion though. At 02:49 PM 6/26/2007, you wrote: >Gayle Callis wrote: "DAB will fluoresce when excited at same wavelength as >FITC." > >My questions are: Assuming that all the parameters were kept equivalent >within a "batch" of slides: > >Can it be used in image analysis to quantify expression of a particular >epitope of interest among different samples in a batch of stained slides? > >Or is DAB precipitate more of a random quantity depending on substrate >availability/enzyme activity and therefore cannot be used to compare >among slides >(I realize that enzyme kinetics make the word "random" a bit of an >contradiction in this sense)? This is based more upon the premise that >the fluorescent >secondary only has so many places to bind vs. HRP that creates more color >the longer it works......(at least until the reaction is stopped or the >substrate/cofactors are exhausted). > >Thanks, >Albert > >Albert C. Grobe, PhD >International Heart Institute of Montana Foundation >Tissue Engineering Lab, Saint Patrick Hospital Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From ladylaynah <@t> yahoo.com Tue Jun 26 21:26:30 2007 From: ladylaynah <@t> yahoo.com (Constance McManus) Date: Tue Jun 26 21:26:40 2007 Subject: [Histonet] HRP Substrates Message-ID: <807917.22030.qm@web37013.mail.mud.yahoo.com> I'm using NOVA Red and it works great. It seems to be about like AEC -- has a rich, deep red color -- except that it goes through alcohols and histoclear nicely. The data sheet states that it needs to be prepared in glass distilled water, which I do. I don't know what would happen if you used regular DI, I imagine it would form a precipitate or not be as rich a red color. It also needs to be prepared within a couple of minutes before using. But it's not difficult to use and I highly recommend it. Connie McManus, HT University of Utah School of Medicine, Dept. of Dermatology >What about NOVA Red from Vector? It withstands alcohols, but how much I am >not certain of. You can contact Craig Pow at VECTOR email >is: cspow@vectorlabs.com >He is tech services and very helpful At 10:28 AM 6/26/2007, you wrote: >Hi all. I am looking for a peroxidase substrate that will withstand paraffin >embedding. Something other that DAB. AEC and TMB are not stable thru >embedding if memory serves me right. Any suggestions? Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Moody friends. Drama queens. Your life? Nope! - their life, your story. Play Sims Stories at Yahoo! Games. http://sims.yahoo.com/ From ladylaynah <@t> yahoo.com Tue Jun 26 21:50:43 2007 From: ladylaynah <@t> yahoo.com (Constance McManus) Date: Tue Jun 26 21:50:52 2007 Subject: [Histonet] melanoma IHC with frozen sections Message-ID: <113144.44852.qm@web37014.mail.mud.yahoo.com> Greetings, all! Is anyone out there doing Microphthalmia Transcription Factor (MiTF) IHC for melanocytes on frozen sections (Mohs surgery)? I am doing skin, frozen sections at 3 um. The problem is inconsistent staining. I embed 2 or 3 pieces of tissue in the same block, but one piece will not stain at all while the other piece[s] will stain beautifully. This doesn't happen every time, and when it does, I can't see where i have done anything different from the protocol. I have taken great care to be certain there is no overlapping OCT on the tissue sections, we put the sections in a 54 C oven for 4 minutes, then acetone fixation for 20 sec. before doing the staining. I am using NeoMarkers reagents: Ultra V blocker One step HRP polymer Neomarkers MiTF Does anyone know anything about using alk phos on skin? Would there be any advantage in switching to AP ? Thanks! Connie McManus, HT University of Utah School of Medicine Dept. of Dermatology ____________________________________________________________________________________ Yahoo! oneSearch: Finally, mobile search that gives answers, not web links. http://mobile.yahoo.com/mobileweb/onesearch?refer=1ONXIC From d.fuster <@t> ub.edu Wed Jun 27 06:35:57 2007 From: d.fuster <@t> ub.edu (Dolors) Date: Wed Jun 27 06:36:18 2007 Subject: [Histonet] Xmatrx Systems Message-ID: <46824B9D.4000702@ub.edu> Any references about Xmatrx system from biogenex? From gvdobbin <@t> ihis.org Wed Jun 27 07:28:33 2007 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Wed Jun 27 07:29:55 2007 Subject: [Histonet] Acidic tap water and automated H&E Message-ID: Hi Folks, It seems we have acidic tap water causing our H&E's to stain purple rather than blue. We have the tap water plumbed into the automatic stainer. Is there a cheap solution here? I am thinking I could add slow dissolving calcium carbonate to the tap water wash buckets to gently raise the pH, but the only source of calcium carbonate that I can come up with that is not in powder form, is pellet lime for lawn applications. I'm not ready to go out and buy a bag of pellet lime just yet. Any other suggestions or thoughts? Surely this is not a new problem. Thanks for listening/reading. Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. Déclaration de confidentialité Le présent message (y compris les annexes) peut contenir des renseignements confidentiels ayant pour objet une personne ou un organisme particulier. Si vous avez reçu la présente communication par erreur, veuillez en informer l'expéditeur immédiatement. Si vous n'êtes pas le destinataire prévu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer complètement de votre système informatique. From talulahgosh <@t> gmail.com Wed Jun 27 08:22:39 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Wed Jun 27 08:22:49 2007 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: We use Southern Biotech Fluoromount G for fluorescense and Biomedia Gel/Mount for other aqueous slides (IHC, ISH) We don't seal them with nail polish and they don't dry out, but other labs on our floor have had a problem with drying out using the same media. I think it may be because we use a lot more mounting media when we coverslip--four to six drops on a slide, rather than three. Biomedia Gel/mount recently changed their data sheet to say NOT to use it with IHC and ISH, but we haven't had a problem. Emily -- penguins, spoons, and you--what's life like among the flightless? http://www.getafirstlife.com/ From relia1 <@t> earthlink.net Wed Jun 27 10:28:49 2007 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Jun 27 10:28:58 2007 Subject: [Histonet] RELIA Histology JOB ALERT!!! Message-ID: Hi Histonetters, I am working with a hospital in Southern VA. The client is located close to the NC border and VA beaches. The best of both worlds, an intimate small lab setting with great benefits due to their recent affiliation with a large hospital system. This is a full time day shift permanent position. This client is motivated to move quickly if you are. If you are interested please contact me via e-mail at relia1@earthlink.net or toll free at 866-607-3542. I am available in the evening if that is more convenient for you. New grads are welcome!!!!! Also if you know someone else who might be interested please feel free to pass this along as well. Thanks again - Pam 866-607-3542 Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net From Dorothy.L.Webb <@t> HealthPartners.Com Wed Jun 27 11:22:05 2007 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Wed Jun 27 11:22:15 2007 Subject: [Histonet] Microwave processing Message-ID: <0E394B648E5284478A6CCB78E5AFDA2703640DD9@hpes1.HealthPartners.int> We were having problems with "friable" prostate needle bx.'s and often uneven staining when using our microwave processor. We were sent a sample of an "EBS" product called "polar sheets" that absorb some of the enery when the tissues are in paraffin until it achieves the higher temperature. This product is incredible..our slides from the microwave processing look better than routine processing stained slides. If anyone needs any information or further testimonial info on this product, contact Phil McArdle with Energy Beam Sciences or I will be happy to assist!! IT'S A MIRACLE!!!!!!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From Ngale <@t> bccancer.bc.ca Wed Jun 27 11:54:07 2007 From: Ngale <@t> bccancer.bc.ca (Gale, Nadia) Date: Wed Jun 27 11:54:13 2007 Subject: [Histonet] Nuclear artefact Message-ID: Urgent help required!!! I know that I've seen previous messages regarding this artefact but need some current views on the subject. I am seeing nuclear artefact in some of our formalin fixed paraffin embedded tissues. The artefact appears as vacuoles or bubbles in the nuclei with H&E. I have tried multiple things to fix this to no avail. Please, please provide your wisdom as to what could be causing this. I am at a loss!!! Many thanks in advance, Nadia Gale Histotechnologist Centre for Translational and Applied Genomics (CTAG) 3427-600 West 10th Avenue Vancouver, BC V5Z 4E6 tel 604-877-6000 ext 2426 fax 604-877-6089 ngale@bccancer.bc.ca From Terry.Marshall <@t> rothgen.nhs.uk Wed Jun 27 11:59:19 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Wed Jun 27 11:59:30 2007 Subject: [Histonet] Nuclear artefact Message-ID: <5C0BED61F529364E86309CADEA63FEF25E6C45@TRFT-EX01.xRothGen.nhs.uk> Look more closely. They are not nuclear. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gale, Nadia Sent: 27 June 2007 17:54 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Nuclear artefact Urgent help required!!! I know that I've seen previous messages regarding this artefact but need some current views on the subject. I am seeing nuclear artefact in some of our formalin fixed paraffin embedded tissues. The artefact appears as vacuoles or bubbles in the nuclei with H&E. I have tried multiple things to fix this to no avail. Please, please provide your wisdom as to what could be causing this. I am at a loss!!! Many thanks in advance, Nadia Gale Histotechnologist Centre for Translational and Applied Genomics (CTAG) 3427-600 West 10th Avenue Vancouver, BC V5Z 4E6 tel 604-877-6000 ext 2426 fax 604-877-6089 ngale@bccancer.bc.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Karen.Heckford <@t> CHW.edu Wed Jun 27 12:08:10 2007 From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF) Date: Wed Jun 27 12:08:05 2007 Subject: [Histonet] PowerPath 9.1 Message-ID: I was wondering if anyone is using the new PowerPath 9.1with Tamtron(Impac)? If so how do you like it? I was just informed we are suppose to be getting this new version with the cassettle labeler and slide labeler with barcode by the end of the year. Your input would be greatly appreciated. Cheers, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathology Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu From jcline <@t> wchsys.org Wed Jun 27 12:13:00 2007 From: jcline <@t> wchsys.org (Joyce Cline) Date: Wed Jun 27 12:12:48 2007 Subject: [Histonet] Slide storage index card dividers/Jackie In-Reply-To: Message-ID: <000501c7b8de$6ab13f80$1d2a14ac@wchsys.org> Hi Jackie, Lab Storage Systems, Inc. 1-800-345-4167. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Tuesday, June 26, 2007 2:03 PM To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] Slide storage index card dividers I know someone knows a couple of vendors where I can get colored pre-cut slide dividers. I've been racking my brain all morning. Someone please rest my brain. Jackie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From contact <@t> excaliburpathology.com Wed Jun 27 12:32:01 2007 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Wed Jun 27 12:32:09 2007 Subject: [Histonet] Roche makes offer to buy Ventana Message-ID: <406315.26583.qm@web50102.mail.re2.yahoo.com> Hello, Roche is offering $75/share or $3 billion to buy Ventana! The price has gone as high as $79 today. If you have any Ventana stock, sell now and make your profit. And, if the deal goes thruogh maybe it will be a kinder, gentler company. Paula From making <@t> ufl.edu Wed Jun 27 12:29:39 2007 From: making <@t> ufl.edu (MKing) Date: Wed Jun 27 12:32:42 2007 Subject: [Histonet] fluorescent peroxidase substrate questions Message-ID: <46829E83.7050300@ufl.edu> Look up tyramine/tyramide, the basis for the signal amplification (TSA) for peroxidase. I think it is fluorescent and a great substrate, or you could use biotin-tyramide to pile biotins at the site, then use avidinylated fluors. As for quantifying expression, you'd have to figure out a way to generate concentration standards and react them like your sections, then you _might_ be able to convince skeptics that your image analysis values were meaningful. It ain't trivial though. --Mike King ----------------------- Date: Tue, 26 Jun 2007 15:37:04 -0400 From: "Turner, Scott" Subject: RE: [Histonet] HRP Substrates To: While we're on the subject of HRP substrates, does anyone know of an HRP substrate that will fluoresce? I know that some Alk-Phos substrates like Vector's AP Red do, but I'm looking for an HRP that will work for a particular IF assay we're working on. Scott Turner Schering-Plough Biopharma Palo Alto, CA ------------------ My questions are: Assuming that all the parameters were kept equivalent within a "batch" of slides: Can it be used in image analysis to quantify expression of a particular epitope of interest among different samples in a batch of stained slides? Or is DAB precipitate more of a random quantity depending on substrate availability/enzyme activity and therefore cannot be used to compare among slides (I realize that enzyme kinetics make the word "random" a bit of an contradiction in this sense)? This is based more upon the premise that the fluorescent secondary only has so many places to bind vs. HRP that creates more color the longer it works......(at least until the reaction is stopped or the substrate/cofactors are exhausted). Thanks, Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital From Jackie.O'Connor <@t> abbott.com Wed Jun 27 12:40:30 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed Jun 27 12:41:20 2007 Subject: [Histonet] Roche makes offer to buy Ventana In-Reply-To: <406315.26583.qm@web50102.mail.re2.yahoo.com> Message-ID: Mmmmmm - insider trading? Isn't that what Martha Stewart went to jail for? Paula Pierce Sent by: histonet-bounces@lists.utsouthwestern.edu 06/27/2007 12:32 PM To Histonet cc Subject [Histonet] Roche makes offer to buy Ventana Hello, Roche is offering $75/share or $3 billion to buy Ventana! The price has gone as high as $79 today. If you have any Ventana stock, sell now and make your profit. And, if the deal goes thruogh maybe it will be a kinder, gentler company. Paula _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JSCHUMA1 <@t> Fairview.org Wed Jun 27 14:01:50 2007 From: JSCHUMA1 <@t> Fairview.org (Schumacher, Jennifer J) Date: Wed Jun 27 14:01:59 2007 Subject: [Histonet] Toxoplasma gondii In-Reply-To: <0E394B648E5284478A6CCB78E5AFDA2703640DD9@hpes1.HealthPartners.int> Message-ID: One of my coworkers is looking for a control block for toxoplasma gondii......willing to trade another tissue block for it. Have plenty of BK/polyoma virus, CMV or anaplastic large cell - to name a few. Please reply directly to: Ann Maruska Lead IHC tech University of Minnesota Medical Center, Fairview 612-273-9119 amarusk1@fairview.org Thank you. Jennifer Jennifer Schumacher CLS Lead, Pathology Riverside Campus University of Minnesota Medical Center, Fairview 2450 Riverside Ave Minneapolis, MN 55454 Phone 612.273.2883 Pager 612.899.6038 Fax 612.273.9124 email jschuma1@fairview.org From bamoe <@t> gundluth.org Wed Jun 27 15:13:13 2007 From: bamoe <@t> gundluth.org (bamoe@gundluth.org) Date: Wed Jun 27 15:13:23 2007 Subject: [Histonet] Silver staining technique Message-ID: Hi all - A question has come up in our laboratory regarding silver staining technique. We currently run special stains manually. For the GMS silver stain, control tissue is run on a separate slide from the patient tissue. During the silver step (we use a microwave for heating) the control slide is checked microscopically for adequate development. The question is this: If the control tissue is developed to satisfaction of the technician, do you automatically remove the patient slides from the silver and continue on with the procedure, OR do you individually check each patient slide, giving some more time in the silver solution if needed? Any and all comments are welcome! Thank you! Barb Moe Gundersen Lutheran Medical Center La Crosse WI From vic <@t> vetmed.wsu.edu Wed Jun 27 15:25:53 2007 From: vic <@t> vetmed.wsu.edu (Leyva-Grado, Victor) Date: Wed Jun 27 15:26:00 2007 Subject: [Histonet] Anti-mouse TNFa antibody on mice brains In-Reply-To: References: Message-ID: <34EFB08480241347BEBE1F095ABB96F4207717@cvm36.vetmed.wsu.edu> Dear members, A friend of mine asked me about a TNFa antibody to be used in mice brains. I've been using the R&D anti-rat recombinant TNFa with very good results. The same company has an anti-mouse TNFa antibody. I tried it once with not very good results in the olfactory bulb of mice (DAB staining). Does anybody has experience with this (R&D)or another anti-mouse TNFa on the mouse brain? Thanks, Victor H Leyva DVM VCAPP Washington State University From jennifer.l.hofecker <@t> Vanderbilt.Edu Wed Jun 27 15:33:27 2007 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Wed Jun 27 15:33:38 2007 Subject: [Histonet] Silver staining technique In-Reply-To: Message-ID: <898D946569A27444B65667A49C074052F727AD@mailbe06.mc.vanderbilt.edu> When manually performing silver stains, I always check each slide (pt and control) for adequate development. In the case of GMS, Bielschowsky, or other stains in which the patient tissue may not contain the elements of interest, still check each slide but if in doubt, stop development when the control slide staining is adequate. That, in my opinion, is an advantage of manual staining. It is far easier to attain optimal staining for each patient sample. We currently do all our NP silver stains by hand and I feel much better giving the docs slides which were individually monitored during development. For the record, I am not opposed to automated SS (just trying to ward off the flames...) I hope this has been of some help. Have a great rest of the week! Jennifer L. Hofecker HT (ASCP) Vanderbilt University Medical Center Neuropathology Lab Nashville, Tennessee ph. (615) 343-0083 fax (615) 343-7089 NSH Quality Control Chairperson -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of bamoe@gundluth.org Sent: Wednesday, June 27, 2007 3:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Silver staining technique Hi all - A question has come up in our laboratory regarding silver staining technique. We currently run special stains manually. For the GMS silver stain, control tissue is run on a separate slide from the patient tissue. During the silver step (we use a microwave for heating) the control slide is checked microscopically for adequate development. The question is this: If the control tissue is developed to satisfaction of the technician, do you automatically remove the patient slides from the silver and continue on with the procedure, OR do you individually check each patient slide, giving some more time in the silver solution if needed? Any and all comments are welcome! Thank you! Barb Moe Gundersen Lutheran Medical Center La Crosse WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From FUNKM <@t> mercyhealth.com Wed Jun 27 16:54:22 2007 From: FUNKM <@t> mercyhealth.com (Marcia Funk) Date: Wed Jun 27 16:54:48 2007 Subject: [Histonet] Log for specimens Message-ID: <4682963E020000AC00003D00@nodcmsngwia1.trinity-health.org> Hello, With the new technology -paper no more- how are most labs documenting to the embedding staff how many specimens should be in the cassette to embed. For example there are three small biopsy in the bag to embed and all need to be accounted for when they are being embedding. With the old embedding log it was hand written by clerk or PA now there are times that the gross description is not available at the time of embedding. The PA could write on the side of the cassette but I was interested in how other folks are following through. Thanks to all for your help I know we are all so busy but it is so helpful to have great information and knowledge from our Histo folks for all of us that have been around since, well you know. Mrf From rsrichmond <@t> aol.com Wed Jun 27 17:06:13 2007 From: rsrichmond <@t> aol.com (rsrichmond@aol.com) Date: Wed Jun 27 17:06:35 2007 Subject: [Histonet] Re: Nuclear artefactI In-Reply-To: <200706271300.94e468297ca9@rly-mf03.mail.aol.com> References: <200706271300.94e468297ca9@rly-mf03.mail.aol.com> Message-ID: <8C9871E82F4E338-1EF8-2201@WEBMAIL-DC12.sysops.aol.com> Nadia Gale, Histotechnologist at the Centre for Translational and Applied Genomics (CTAG) in Vancouver, BC asks: >>I am seeing nuclear artefact in some of our formalin fixed paraffin embedded tissues. The artefact appears as vacuoles or bubbles in the nuclei with H&E. I have tried multiple things to fix this to no avail.<< I think we've discussed this topic before on Histonet, and maybe I just wasn't paying attention - but I would really like to know how to trouble shoot this common artifact: just what do you do first, and next, and after that? In my travels as a locum tenens pathologist this is one of the most common problems I see in laboratories. Always everybody is in denial that any such thing exists. In particular, this bubble artifact makes it impossible to assess the nuclear grade of breast cancers and other tumors requiring nuclear grading. Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From Sarah.Jones <@t> dako.com Wed Jun 27 17:19:10 2007 From: Sarah.Jones <@t> dako.com (Sarah Jones) Date: Wed Jun 27 17:19:26 2007 Subject: [Histonet] Re: Nuclear artefactI In-Reply-To: <8C9871E82F4E338-1EF8-2201@WEBMAIL-DC12.sysops.aol.com> References: <200706271300.94e468297ca9@rly-mf03.mail.aol.com> <8C9871E82F4E338-1EF8-2201@WEBMAIL-DC12.sysops.aol.com> Message-ID: <8B07D141BCDE434285DC12B3290E3FB3012F79A7@exbackca.caus.dako.net> Dear Samurai Pathologist and other interested folks: Here is the answer to your question about nuclear bubbling artifact: http://www.anatechltdusa.com/Innovators/2_Innartifact.html Best regards, Sarah A. Jones, HTL(ASCP)CM -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of rsrichmond@aol.com Sent: Wednesday, June 27, 2007 3:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Nuclear artefactI Nadia Gale, Histotechnologist at the Centre for Translational and Applied Genomics (CTAG) in Vancouver, BC asks: >>I am seeing nuclear artefact in some of our formalin fixed paraffin embedded tissues. The artefact appears as vacuoles or bubbles in the nuclei with H&E. I have tried multiple things to fix this to no avail.<< I think we've discussed this topic before on Histonet, and maybe I just wasn't paying attention - but I would really like to know how to trouble shoot this common artifact: just what do you do first, and next, and after that? In my travels as a locum tenens pathologist this is one of the most common problems I see in laboratories. Always everybody is in denial that any such thing exists. In particular, this bubble artifact makes it impossible to assess the nuclear grade of breast cancers and other tumors requiring nuclear grading. Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Wed Jun 27 19:23:44 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Jun 27 19:24:00 2007 Subject: [Histonet] Silver staining technique References: Message-ID: <006501c7b91a$98470d30$d49eae18@yourxhtr8hvc4p> I've been burned in the past (I'm not talking about being flamed now) by just checking the control slide without checking the patient. Like most other places, we cut controls in batches. Some one else might cut the patient. Different techs, different cutting techniques, etc. Me, myself, have performed GMS stains were I just looked at the control (which was perfect I might add) and the patient was over stained because it was loaded with fungi more than the control did. People say I'm a fun guy or was that fungi? Either way, I have a tendency to grow on people. Joe ----- Original Message ----- From: To: Sent: Wednesday, June 27, 2007 3:13 PM Subject: [Histonet] Silver staining technique > > Hi all - > > A question has come up in our laboratory regarding silver staining > technique. > > We currently run special stains manually. For the GMS silver stain, > control tissue is run on a separate slide from the patient tissue. During > the silver step (we use a microwave for heating) the control slide is > checked microscopically for adequate development. > > The question is this: > > If the control tissue is developed to satisfaction of the technician, do > you automatically remove the patient slides from the silver and continue > on > with the procedure, OR do you individually check each patient slide, > giving some more time in the silver solution if needed? > > Any and all comments are welcome! > > Thank you! > > Barb Moe > Gundersen Lutheran Medical Center > La Crosse WI > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Wed Jun 27 19:34:57 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Jun 27 19:35:31 2007 Subject: [Histonet] Silver staining technique In-Reply-To: <006501c7b91a$98470d30$d49eae18@yourxhtr8hvc4p> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FF9CFC@sjhaexc02.sjha.org> Oh no! Toe fungi... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, June 27, 2007 8:24 PM To: bamoe@gundluth.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Silver staining technique I've been burned in the past (I'm not talking about being flamed now) by just checking the control slide without checking the patient. Like most other places, we cut controls in batches. Some one else might cut the patient. Different techs, different cutting techniques, etc. Me, myself, have performed GMS stains were I just looked at the control (which was perfect I might add) and the patient was over stained because it was loaded with fungi more than the control did. People say I'm a fun guy or was that fungi? Either way, I have a tendency to grow on people. Joe ----- Original Message ----- From: To: Sent: Wednesday, June 27, 2007 3:13 PM Subject: [Histonet] Silver staining technique > > Hi all - > > A question has come up in our laboratory regarding silver staining > technique. > > We currently run special stains manually. For the GMS silver stain, > control tissue is run on a separate slide from the patient tissue. During > the silver step (we use a microwave for heating) the control slide is > checked microscopically for adequate development. > > The question is this: > > If the control tissue is developed to satisfaction of the technician, do > you automatically remove the patient slides from the silver and continue > on > with the procedure, OR do you individually check each patient slide, > giving some more time in the silver solution if needed? > > Any and all comments are welcome! > > Thank you! > > Barb Moe > Gundersen Lutheran Medical Center > La Crosse WI > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From Kemlo.Rogerson <@t> waht.swest.nhs.uk Thu Jun 28 01:44:46 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Jun 28 01:44:55 2007 Subject: [Histonet] Re: Nuclear artefactI Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222E7CD@wahtntex2.waht.swest.nhs.uk> Agree with Terry, the bubbles are not nuclear, and yes the problem is fixation and yes we've discussed it many times. Fix longer with adequate amounts of formalin or use another fixative although I prefer the former. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; "The true meaning of life is to plant trees, under whose shade you do not expect to sit. --Nelson Henderson This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From TMcNemar <@t> lmhealth.org Thu Jun 28 05:08:06 2007 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Thu Jun 28 05:08:15 2007 Subject: [Histonet] Log for specimens In-Reply-To: <4682963E020000AC00003D00@nodcmsngwia1.trinity-health.org> Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F4A6@lmhsmail.lmhealth.org> We have a small form (1/2 sheet) that is carboned for multiple copies. The grossing person just writes any special instructions (embedding, stains, etc.) on it. We then have have it for embedding and a copy for each cutter. Works very well.Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Marcia Funk Sent: Wednesday, June 27, 2007 5:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Log for specimens Hello, With the new technology -paper no more- how are most labs documenting to the embedding staff how many specimens should be in the cassette to embed. For example there are three small biopsy in the bag to embed and all need to be accounted for when they are being embedding. With the old embedding log it was hand written by clerk or PA now there are times that the gross description is not available at the time of embedding. The PA could write on the side of the cassette but I was interested in how other folks are following through. Thanks to all for your help I know we are all so busy but it is so helpful to have great information and knowledge from our Histo folks for all of us that have been around since, well you know. Mrf _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dlcowie <@t> prodigy.net Thu Jun 28 05:31:58 2007 From: dlcowie <@t> prodigy.net (Dawn Cowie) Date: Thu Jun 28 05:32:08 2007 Subject: [Histonet] Log for specimens In-Reply-To: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F4A6@lmhsmail.lmhealth.org> Message-ID: <311807.45561.qm@web81009.mail.mud.yahoo.com> We have the PA's write on the side of the cassette the number of pieces that are in the cassette. This is especially helpful if the pieces are very small, you know when you open the cassette how many pieces you are looking for. We have an exception log that we write in if there are missing pieces at embedding. This saves time from having to stop and check a paper log for each cassette. DLC Tom McNemar wrote: We have a small form (1/2 sheet) that is carboned for multiple copies. The grossing person just writes any special instructions (embedding, stains, etc.) on it. We then have have it for embedding and a copy for each cutter. Works very well.Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Marcia Funk Sent: Wednesday, June 27, 2007 5:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Log for specimens Hello, With the new technology -paper no more- how are most labs documenting to the embedding staff how many specimens should be in the cassette to embed. For example there are three small biopsy in the bag to embed and all need to be accounted for when they are being embedding. With the old embedding log it was hand written by clerk or PA now there are times that the gross description is not available at the time of embedding. The PA could write on the side of the cassette but I was interested in how other folks are following through. Thanks to all for your help I know we are all so busy but it is so helpful to have great information and knowledge from our Histo folks for all of us that have been around since, well you know. Mrf _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Thu Jun 28 07:12:46 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Thu Jun 28 07:13:16 2007 Subject: [Histonet] Re: Nuclear artefactI Message-ID: <5C0BED61F529364E86309CADEA63FEF25E6C4E@TRFT-EX01.xRothGen.nhs.uk> That everyone is in denial is exactly my experience. It also makes it impossible to diagnose small cell carcinoma without resorting to immunochemistry:-) Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of rsrichmond@aol.com Sent: 27 June 2007 23:06 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Nuclear artefactI Nadia Gale, Histotechnologist at the Centre for Translational and Applied Genomics (CTAG) in Vancouver, BC asks: >>I am seeing nuclear artefact in some of our formalin fixed paraffin embedded tissues. The artefact appears as vacuoles or bubbles in the nuclei with H&E. I have tried multiple things to fix this to no avail.<< I think we've discussed this topic before on Histonet, and maybe I just wasn't paying attention - but I would really like to know how to trouble shoot this common artifact: just what do you do first, and next, and after that? In my travels as a locum tenens pathologist this is one of the most common problems I see in laboratories. Always everybody is in denial that any such thing exists. In particular, this bubble artifact makes it impossible to assess the nuclear grade of breast cancers and other tumors requiring nuclear grading. Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaina.negandhi <@t> sickkids.ca Thu Jun 28 08:45:36 2007 From: jaina.negandhi <@t> sickkids.ca (jaina.negandhi@sickkids.ca) Date: Thu Jun 28 08:45:47 2007 Subject: [Histonet] c-fos staining Message-ID: I am carrying out c-fos staining on brain tissue, and have run into a bit of a problem. The staining seems to work fine up untill I mount the sample with a xylene based mountant (permount). The stains are there after alcohol washing, but once I mount it, the stains seem to disappear, or become very very faint. Any ideas as to why this is happening, and what I can do about it. Thanks, Jaina Negandhi Research Project Coordinator Neuroscience and Mental Health Hospital for Sick Children Mc Master Building, RM 3006 Tel: 416-813-6551 Fax: 416-813-8724 From LINDA.MARGRAF <@t> childrens.com Thu Jun 28 09:21:57 2007 From: LINDA.MARGRAF <@t> childrens.com (LINDA MARGRAF) Date: Thu Jun 28 09:22:20 2007 Subject: [Histonet] job opportunity in Dallas Message-ID: <46837DB5020000DA0000E8F3@CNET3.CHILDRENS.COM> Here's a job opportunity from the University of Texas Southwestern Medical School my colleagues asked me to post (please contact them not me) Thanks.... Histology Tech - Biology Dept of UTSW Medical Center This is a Full Time, Monday through Friday, 8 AM - 5 PM position, no weekends. The technician besides being responsible for the typical registry eligible Histo Tech job duties, the technician interfaces with 1st yr medical students, maintains their microscopes and other related job duties. Please email your resume to: conchita.delgado@utsouthwestern.edu OR Call Conchita Delgado, Senior Recruiter, at 214 648 9818 From Rcartun <@t> harthosp.org Thu Jun 28 09:24:52 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Jun 28 09:25:10 2007 Subject: [Histonet] Question - HercepTest Message-ID: <46838C740200007700006A1F@gwmail.harthosp.org> For those of you who are using Dako's HercepTest kit for the IHC detection of HER2 in breast CA, has Dako changed the interpretation guidelines? Meaning is "2+" now called "Equivocal" (as recommended by the ASCO/CAP guidelines published in January of this year) or is it still considered "Positive"? Thanks. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From pmarcum <@t> vet.upenn.edu Thu Jun 28 09:43:47 2007 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Thu Jun 28 09:43:58 2007 Subject: [Histonet] Looking for information for a friend Message-ID: <6.2.5.6.2.20070628103650.01c6ebb0@vet.upenn.edu> Hi All, I had a friend call me last night and ask if could make a request of the HistoNet community in the Columbus OH area. She has years of experience as histologist and IHC specialist in both small and large labs. She has been a manager and help start several labs over the years. Currently she is moving and has not immediate computer access or she would have done this herself. Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From bwhitaker <@t> brownpathology.com Thu Jun 28 09:46:33 2007 From: bwhitaker <@t> brownpathology.com (Bonnie Whitaker) Date: Thu Jun 28 09:44:48 2007 Subject: [Histonet] Log for specimens In-Reply-To: <311807.45561.qm@web81009.mail.mud.yahoo.com> Message-ID: <002c01c7b993$1fb16900$3601a8c0@brownpathology.net> One thing that comes to mind as a possibility (I still use paper) is colored glass beads dropped in the cassette (such as red=2, blue =3, green=4, etc.) I use that system to alert the embedding tech(s) for any special instructions on a cassette now. Bonnie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawn Cowie Sent: Thursday, June 28, 2007 5:32 AM To: Tom McNemar; Marcia Funk; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Log for specimens We have the PA's write on the side of the cassette the number of pieces that are in the cassette. This is especially helpful if the pieces are very small, you know when you open the cassette how many pieces you are looking for. We have an exception log that we write in if there are missing pieces at embedding. This saves time from having to stop and check a paper log for each cassette. DLC Tom McNemar wrote: We have a small form (1/2 sheet) that is carboned for multiple copies. The grossing person just writes any special instructions (embedding, stains, etc.) on it. We then have have it for embedding and a copy for each cutter. Works very well.Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Marcia Funk Sent: Wednesday, June 27, 2007 5:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Log for specimens Hello, With the new technology -paper no more- how are most labs documenting to the embedding staff how many specimens should be in the cassette to embed. For example there are three small biopsy in the bag to embed and all need to be accounted for when they are being embedding. With the old embedding log it was hand written by clerk or PA now there are times that the gross description is not available at the time of embedding. The PA could write on the side of the cassette but I was interested in how other folks are following through. Thanks to all for your help I know we are all so busy but it is so helpful to have great information and knowledge from our Histo folks for all of us that have been around since, well you know. Mrf _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LuckG <@t> empirehealth.org Thu Jun 28 10:00:13 2007 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Thu Jun 28 10:00:29 2007 Subject: [Histonet] Looking for Kari Rigg Message-ID: <6BB8BC4519AAB844B174FC739A679BBCCEFE29@IRMEXCH01.irm.inhs.org> Hello All, I am trying to locate a pathologist's assistant named Kari Rigg. My understanding is that she may be currently employed in Texas. She worked at Lovelace Medical Center in Albuquerque before that. Thanks, Greg Greg Luck, B.S., HT(ASCP) Anatomic Pathology Supervisor Deaconess Med Cntr 800 W. 5th Ave Spokane, WA 99204 Offc 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconessmc.org From oshel1pe <@t> cmich.edu Thu Jun 28 10:11:16 2007 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Thu Jun 28 10:11:27 2007 Subject: [Histonet] c-fos staining In-Reply-To: References: Message-ID: Jaina, Most likely it's the Permount (maybe the xylenes). Use an aqueous mount like glycerol, methyl salicylate*, Aquamount, Fluormount, etc. There are others that I don't recall off hand. After mounting, keep the slides flat. Seal with VALAP. *Methyl salicylate is an excellent clearing medium and mountant, go into it using your xylenes schedule. We have specimens that have been in Me-sal for 14+ years and are still nice and bright. Rhodamine-Phalloidin stain, not slide mounted but kept in e.g. microfuge tubes. These aren't brain sections, but it should work for them, too. Phil >I am carrying out c-fos staining on brain tissue, and have run into a bit >of a problem. The staining seems to work fine up untill I mount the sample >with a xylene based mountant (permount). The stains are there after alcohol >washing, but once I mount it, the stains seem to disappear, or become very >very faint. > >Any ideas as to why this is happening, and what I can do about it. > >Thanks, > >Jaina Negandhi >Research Project Coordinator >Neuroscience and Mental Health >Hospital for Sick Children >Mc Master Building, RM 3006 >Tel: 416-813-6551 >Fax: 416-813-8724 -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 From TMcNemar <@t> lmhealth.org Thu Jun 28 10:17:13 2007 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Thu Jun 28 10:17:26 2007 Subject: [Histonet] Silver staining technique In-Reply-To: Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F4A7@lmhsmail.lmhealth.org> We run a positive control on the same slide for each patient. We typically stain for the control. If the control looks good, we take it out. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of bamoe@gundluth.org Sent: Wednesday, June 27, 2007 4:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Silver staining technique Hi all - A question has come up in our laboratory regarding silver staining technique. We currently run special stains manually. For the GMS silver stain, control tissue is run on a separate slide from the patient tissue. During the silver step (we use a microwave for heating) the control slide is checked microscopically for adequate development. The question is this: If the control tissue is developed to satisfaction of the technician, do you automatically remove the patient slides from the silver and continue on with the procedure, OR do you individually check each patient slide, giving some more time in the silver solution if needed? Any and all comments are welcome! Thank you! Barb Moe Gundersen Lutheran Medical Center La Crosse WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Thu Jun 28 10:26:20 2007 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Jun 28 10:26:31 2007 Subject: [Histonet] Looking for an old friend - Eli George Message-ID: Hi Histonetters! I hope everyone is having a great day. I am trying to locate an old friend of mine a histo tech named Eli George. The last I heard he was based out of CT and doing temp work. If anyone knows how to reach him I would really appreciate being put in contact with him. Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net From gu.lang <@t> gmx.at Thu Jun 28 10:40:03 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Jun 28 10:40:13 2007 Subject: [Histonet] SISH - Ventana Message-ID: <002501c7b99a$98c22170$6412a8c0@dielangs.at> I would be interested in people's experience with the SISH-test on the Ventana-benchmark. SISH = Silver in situ hybridisierung for detecting Her2neu-gene copies. The principle is metallography, where the bound HRP catalyses the reduction of Silveracetat. This results in black dots, that can be detected with a light microscope. Thanks in advance Gudrun Lang From TJJ <@t> Stowers-Institute.org Thu Jun 28 10:40:43 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Thu Jun 28 10:41:05 2007 Subject: [Histonet] Re: Silver staining technique Message-ID: Regarding whether to stain for the proper intensity on the control only, or also monitor the patient samples, there are times when stopping the staining because the control was perfect is inappropriate. For instance, some labs do not have a pneumocystis control, and when staining for this microbe, most samples need slightly longer time in the silver than if you were demonstrating aspergillus (commonly used as a fungus control for GMS). Additionally, patients with large balls of fungus in their tissues show normal to weak staining of the fungus as compared to the control, and the center of the fungal colony is almost always very weakly or not stained. In this case, it is helpful to leave the slides in a little longer to try to additionally demonstrate as much of the material as you can (without going too far, of course!). Section thickness can also play a role in staining intensity for some special stains. When possible, always screen the patient slides for positivity. It also gives you a heads up more potential positive control material is available. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From Ngale <@t> bccancer.bc.ca Thu Jun 28 11:05:12 2007 From: Ngale <@t> bccancer.bc.ca (Gale, Nadia) Date: Thu Jun 28 11:05:21 2007 Subject: [Histonet] Nuclear artefact II Message-ID: I've looked at everyone's suggestions/comments and am sad to report that I cannot get rid of the nuclear "bubble" artefact from our slides. I have tried - decreasing water bath temp - increasing/decreasing drying oven time before H&E staining - varying fixation times in formalin I am currently trying - decreasing drying oven temperature - changing the "wrap" that I use for the biopsies when grossing - looking at our wax times on the processor I agree with Bob that it's extremely frustrating that our community cannot seem to agree on a common cause for this (what I am now realizing) common artefact. I'll keep you posted with my results. Thank you so much for all of your ideas and help with this, I am most grateful. Sincerely, Nadia Gale Histotechnologist Centre for Translational and Applied Genomics (CTAG) 3427-600 West 10th Avenue Vancouver, BC V5Z 4E6 tel 604-877-6000 ext 2426 fax 604-877-6089 ngale@bccancer.bc.ca From derek.papalegis <@t> tufts.edu Thu Jun 28 13:40:02 2007 From: derek.papalegis <@t> tufts.edu (Derek Papalegis) Date: Thu Jun 28 13:40:11 2007 Subject: [Histonet] Iron Stain Message-ID: <46840082.2010807@tufts.edu> Am I mistaken or is the Prussian Blue and Perl's Iron stain the same thing? Can someone provide some insight on this? -Derek From denise.woodward <@t> uconn.edu Thu Jun 28 14:30:13 2007 From: denise.woodward <@t> uconn.edu (Woodward, Denise) Date: Thu Jun 28 14:30:22 2007 Subject: [Histonet] job opportunity in IL? In-Reply-To: <46837DB5020000DA0000E8F3@CNET3.CHILDRENS.COM> References: <46837DB5020000DA0000E8F3@CNET3.CHILDRENS.COM> Message-ID: Posting for a co-worker who wishes to return home to IL: Experienced, HTL eligible (ASCP test date scheduled), Histotech available for FT position after July 27th. IHC experienced. Please contact Sarah at (860)486-2745 From Tracey.Lenek <@t> CLS.ab.ca Thu Jun 28 15:03:08 2007 From: Tracey.Lenek <@t> CLS.ab.ca (Tracey.Lenek@CLS.ab.ca) Date: Thu Jun 28 15:03:27 2007 Subject: [Histonet] Ventana Message-ID: <4BC300747AF87A48BCDF8E48BC2885CE040BBC7E@mail1.calgary.com> Hi, Has anyone heard thru the grapevine that Ventana is in the process of being taken over? Tracey CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. From Annette_hall <@t> pa-ucl.com Thu Jun 28 15:55:59 2007 From: Annette_hall <@t> pa-ucl.com (Annette Hall) Date: Thu Jun 28 15:56:16 2007 Subject: [Histonet] Ventana Message-ID: <9FC023A4AB52BB4D87DC6456081A822C012B5F1F@mercury.pa-ucl.com> Roche Announces $3 Billion Offer to Purchase Ventana Medical Systems Yesterday, Roche Holdings (SWX: ROG.VX; RO.S) of Basel, Switzerland announced that it would initiate a tender offer to acquire 100% ownership of Ventana Medical Systems (NASDAQ: VMSI) of Tucson, Arizona. Roche is offering $75 for each share of Ventana Medical. This is a 44% premium over Ventana's closing price of $51.95 on July 22, 2007. It also represents a total cash purchase price of $3 billion for Ventana Medical Systems. For 2006, Ventana Medical Systems had revenue of $238.2 million. Roche's offer is thus based on a large multiple of EBITDA (Earnings Before Interest, Taxes, Depreciation, and Amortization). Roche's offer is unwelcome at Ventana Medical Systems. Executives at Ventana have been tight-lipped since news of the Roche tender offer became public. Earlier this morning, Ventana Medical issued a press release recommending that its shareholders take no action in response to the Roche offer. In the press release, it further stated that its board of directors would consult with financial and legal advisors on the situation. Within 10 days, it will make a recommendation to shareholders. In a conference call yesterday, Roche disclosed that it has approached Ventana Medical Systems numerous times since the first of the year to initiate discussions about acquiring Ventana Medical. These approaches were rebuffed by Ventana's board and executive team. That is why Roche decided to launch a hostile tender offer to acquire all the shares of Ventana. Lab directors and pathologists should not be surprised if another company enters the bidding for Ventana Medical Systems. It has a robust product line in histopathology, a worldwide presence, and there is great respect for the quality of its products and its corporate culture. Because molecular diagnostics is growing at twice the rate of the traditional in vitro diagnostics market, a company like Ventana Medical Systems becomes a highly-desirable acquisition candidate. Stay tuned to Dark Daily. Not only will there be ongoing developments in this hostile takeover action, but it is likely that this story will take some surprising twists before it is resolved. -----Original Message----- From: Tracey.Lenek@CLS.ab.ca [mailto:Tracey.Lenek@CLS.ab.ca] Sent: Thursday, June 28, 2007 3:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana Hi, Has anyone heard thru the grapevine that Ventana is in the process of being taken over? Tracey CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From PMonfils <@t> Lifespan.org Thu Jun 28 16:05:34 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu Jun 28 16:05:47 2007 Subject: [Histonet] Iron Stain In-Reply-To: <46840082.2010807@tufts.edu> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273CAC@LSRIEXCH1.lsmaster.lifespan.org> Yes, Prussian Blue is the pigment formed in the Perl Reaction. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Derek Papalegis > Sent: Thursday, June 28, 2007 2:40 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Iron Stain > > Am I mistaken or is the Prussian Blue and Perl's Iron stain the same > thing? Can someone provide some insight on this? > > -Derek > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From linhines <@t> yahoo.com Thu Jun 28 17:48:04 2007 From: linhines <@t> yahoo.com (Linda Hines) Date: Thu Jun 28 17:48:11 2007 Subject: [Histonet] Eosin on processor Message-ID: <360345.28393.qm@web55414.mail.re4.yahoo.com> I would like to know which of the labs are using Eosin on their tissue processor to mark the small specimens. Also if you have a procedure, if it has interfered with your processing, and the staining of your H&E. I've seen this before and liked it when working with the finite, and some of the needle bx's. This is just one of my sudgestions for our lab. Thanx for your input. Linda TRMC --------------------------------- It's here! Your new message! Get new email alerts with the free Yahoo! Toolbar. From jnocito <@t> satx.rr.com Thu Jun 28 18:31:30 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Jun 28 18:31:44 2007 Subject: [Histonet] Eosin on processor References: <360345.28393.qm@web55414.mail.re4.yahoo.com> Message-ID: <001d01c7b9dc$754fdba0$d49eae18@yourxhtr8hvc4p> Linda, my last lab put 25 mls of Eosin in the first 100% alcohol container on each of the tissue processors. It didn't interfere with the H&E staining, it did stain up the tissue processors, but wasn't a factor as to their operations. Since the lab processed mostly biopsies, the Eosin was a life saver, especially when it came to GI and prostate biopsies. Hope this helps. Joe ----- Original Message ----- From: "Linda Hines" To: Sent: Thursday, June 28, 2007 5:48 PM Subject: [Histonet] Eosin on processor >I would like to know which of the labs are using Eosin on their tissue >processor to mark the small specimens. Also if you have a procedure, if it >has interfered with your processing, and the staining of your H&E. I've >seen this before and liked it when working with the finite, and some of the >needle bx's. This is just one of my sudgestions for our lab. Thanx for >your input. > Linda > TRMC > > > --------------------------------- > It's here! Your new message! > Get new email alerts with the free Yahoo! Toolbar. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Greg.Bowlay <@t> mater.org.au Thu Jun 28 18:42:09 2007 From: Greg.Bowlay <@t> mater.org.au (Bowlay, Greg) Date: Thu Jun 28 18:42:21 2007 Subject: [Histonet] SISH - Ventana References: <002501c7b99a$98c22170$6412a8c0@dielangs.at> Message-ID: <499E3F06E3FDA043801D7ACB71B055220174B8AF@matexch1.mater.org.au> Hi Gudrun, We have been trialling HER2/Ch17 SISH on a Ventana Benchmark and it works very well!! We are very impressed by the contrast between signal and counterstain, particularly in the polysomy amd low amp cases. Another advantage is that the tissue architecture is maintained greatly, and also inclusion of control tissue on same slide. At around six hours for the complete run it increases our TAT's and volume. We are looking at Extended CC2 and four minutes ISH protease 3 for our tissue - which preserves tissue integrity compared to the technique we currently use for CISH (Zymed). We recommend it highly!!! Regards Greg Bowlay Scientist Anatomical Pathology Mater Health Services South Brisbane Q 4101 Greg.Bowlay@mater.org.au PH: +61 7 3840 8586 FAX: +61 7 3840 8338 "Far better it is to dare mighty things to win glorious triumphs even though chequered by failure, than to rank with those poor spirits who neither enjoy nor suffer much because they live in the grey twilight that knows neither victory nor defeat." Theodore Roosevelt ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Gudrun Lang Sent: Fri 29/6/07 1:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] SISH - Ventana I would be interested in people's experience with the SISH-test on the Ventana-benchmark. SISH = Silver in situ hybridisierung for detecting Her2neu-gene copies. The principle is metallography, where the bound HRP catalyses the reduction of Silveracetat. This results in black dots, that can be detected with a light microscope. Thanks in advance Gudrun Lang This e-mail, together with any attachments, is confidential and intended for the named recipient(s) only. If you are not the intended recipient or have received this message in error, you are asked to immediately notify the sender and delete this message and any copies of this message from your computer system network and destroy any printed copies of this email. Any form of unauthorised disclosure, modification, distribution, publication or use of this e-mail message is prohibited. From lpwenk <@t> sbcglobal.net Thu Jun 28 18:55:06 2007 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Jun 28 18:55:44 2007 Subject: [Histonet] Repair of ultramicrotome Message-ID: <001001c7b9df$c1d5d350$0202a8c0@HPPav2> Could someone recommend, or vendors reply to, my need for someone able to repair a Sorvall Dupont MT 5000 ultramicrotome. The Visutrac and the action of the block arm to coordinate is way off. In other words, instead of slowing down during the interval where the diamond knife edge is, the block arm will: - sometimes slow down, - sometimes continue at the same speed, - sometimes slow down in the area above or below the knife edge but go at the fast speed at the knife edge, and, to make it even more fun - sometimes the block arm will reverse direction - go UP instead of continuing down, but just right around the knife edge. Sort of fascinating to watch, as you never know what it is going to do. But this makes it impossible to cut sections. We've tried turning the ultramicrotome on and off, and changed the Visutrac setting, and altered the nanometer thickness (600 angstroms and 1 um) - nothing seems to make a difference. I'm guessing it's a "computer glitch" in the machine, and I'm not about it try to open up the ultramicrome to fix it (coward, I know). If you could email it to my work address (pwenk@beaumont.edu), instead of just replying to this email (home), I would get your reply faster. Thanks in advance. I'm hoping the Histonetters can come through for me on this, as you have on other of my requests. Don't you just love this community? Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 From lpwenk <@t> sbcglobal.net Thu Jun 28 19:20:25 2007 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Jun 28 19:20:49 2007 Subject: [Histonet] Nuclear artefact II In-Reply-To: Message-ID: <001101c7b9e3$4ae784b0$0202a8c0@HPPav2> >From what I've read about nuclear bubbling, it all starts with inadequate fixation. Anything else we do to the tissue just makes it worse. Nothing we can do will ever improve poorly fixed tissue. If there are not enough cross-links of formalin (or any other fixative) due to underfixation, then these few cross-links can be easily broken later on. This causes the proteins and DNA being easily moved around into new locations. So some of the things that can move the proteins and DNA are: - processing (going through alcohols and xylene will warp these under cross-linked proteins/DNA even more) - too much heat in processing (alcohols, xylene, paraffin) - too long of time in hot paraffin - too much heat during slide drying The reason we see this artifact mostly with formalin fixed tissue is two fold: 1) Formalin needs 1-2 days to completely fix 3 mm thick tissue sections. In other words, we need that amount of time to create enough cross-links so that nuclear bubbling and other protein deformities do not occur later with processing, heat, etc. 2) Formalin is a lousy nuclear fixative. Mercury and Zinc are much better fixatives for nuclei. And tend to fix faster than formalin. So B5, Zenker, Zinc-Formalin are less likely to show nuclear bubbling. However, the worst case of nuclear bubbling I ever saw (orphan annie eyes) was in bone marrow biopsies that were underfixed in zinc formalin (tech didn't want to wait the usual amount of time, so only let them fix about half the time required). So, increasing formalin fixative by one hour is NOT going to correct this problem. It's going to take several hours increase in time in formalin before some decrease in nuclear bubbling is seen. So leave the tissue on the processor in the formalin as long as possible at the beginning of the cycle, NOT in the hot paraffin at the end of the cycle. After that, watch the temperatures during processing (keeping at 37 degrees C would be better than 42 degrees, for example for the alcohols/xylene; keep paraffin as low as possible (2 degrees above melting point)). Keep the temperature on the slide dryer down too. 60 degrees C for 30 minutes will be better than 80 degrees C for 15 minutes. And tell the people grossing that they MUST cut thin sections (thickness of a nickle = 2 mm), and NOT overstuff the cassettes. These will not allow formalin to flow around the tissue. So the tissue won't get fixed! So no cross-links, so no protein/DNA stabilization. Ergo, another reason for nuclear bubbling! Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gale, Nadia Sent: Thursday, June 28, 2007 12:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Nuclear artefact II I've looked at everyone's suggestions/comments and am sad to report that I cannot get rid of the nuclear "bubble" artefact from our slides. I have tried - decreasing water bath temp - increasing/decreasing drying oven time before H&E staining - varying fixation times in formalin I am currently trying - decreasing drying oven temperature - changing the "wrap" that I use for the biopsies when grossing - looking at our wax times on the processor I agree with Bob that it's extremely frustrating that our community cannot seem to agree on a common cause for this (what I am now realizing) common artefact. I'll keep you posted with my results. Thank you so much for all of your ideas and help with this, I am most grateful. Sincerely, Nadia Gale Histotechnologist Centre for Translational and Applied Genomics (CTAG) 3427-600 West 10th Avenue Vancouver, BC V5Z 4E6 tel 604-877-6000 ext 2426 fax 604-877-6089 ngale@bccancer.bc.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Thu Jun 28 19:43:14 2007 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Jun 28 19:43:36 2007 Subject: [Histonet] Nuclear artefact II Message-ID: It has also been suggested that this nuclear bubbling could also be due to "boiling-off" of water under the section when it is placed on a hot plate or oven for drying (usually too soon after picking-up of the section from the waterbath). Try allowing the sections to dry at room temp prior to 60oC drying. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Friday, 29 June 2007 10:20 AM To: 'Gale, Nadia'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Nuclear artefact II >From what I've read about nuclear bubbling, it all starts with >inadequate fixation. Anything else we do to the tissue just makes it worse. Nothing we can do will ever improve poorly fixed tissue. If there are not enough cross-links of formalin (or any other fixative) due to underfixation, then these few cross-links can be easily broken later on. This causes the proteins and DNA being easily moved around into new locations. So some of the things that can move the proteins and DNA are: - processing (going through alcohols and xylene will warp these under cross-linked proteins/DNA even more) - too much heat in processing (alcohols, xylene, paraffin) - too long of time in hot paraffin - too much heat during slide drying The reason we see this artifact mostly with formalin fixed tissue is two fold: 1) Formalin needs 1-2 days to completely fix 3 mm thick tissue sections. In other words, we need that amount of time to create enough cross-links so that nuclear bubbling and other protein deformities do not occur later with processing, heat, etc. 2) Formalin is a lousy nuclear fixative. Mercury and Zinc are much better fixatives for nuclei. And tend to fix faster than formalin. So B5, Zenker, Zinc-Formalin are less likely to show nuclear bubbling. However, the worst case of nuclear bubbling I ever saw (orphan annie eyes) was in bone marrow biopsies that were underfixed in zinc formalin (tech didn't want to wait the usual amount of time, so only let them fix about half the time required). So, increasing formalin fixative by one hour is NOT going to correct this problem. It's going to take several hours increase in time in formalin before some decrease in nuclear bubbling is seen. So leave the tissue on the processor in the formalin as long as possible at the beginning of the cycle, NOT in the hot paraffin at the end of the cycle. After that, watch the temperatures during processing (keeping at 37 degrees C would be better than 42 degrees, for example for the alcohols/xylene; keep paraffin as low as possible (2 degrees above melting point)). Keep the temperature on the slide dryer down too. 60 degrees C for 30 minutes will be better than 80 degrees C for 15 minutes. And tell the people grossing that they MUST cut thin sections (thickness of a nickle = 2 mm), and NOT overstuff the cassettes. These will not allow formalin to flow around the tissue. So the tissue won't get fixed! So no cross-links, so no protein/DNA stabilization. Ergo, another reason for nuclear bubbling! Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gale, Nadia Sent: Thursday, June 28, 2007 12:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Nuclear artefact II I've looked at everyone's suggestions/comments and am sad to report that I cannot get rid of the nuclear "bubble" artefact from our slides. I have tried - decreasing water bath temp - increasing/decreasing drying oven time before H&E staining - varying fixation times in formalin I am currently trying - decreasing drying oven temperature - changing the "wrap" that I use for the biopsies when grossing - looking at our wax times on the processor I agree with Bob that it's extremely frustrating that our community cannot seem to agree on a common cause for this (what I am now realizing) common artefact. I'll keep you posted with my results. Thank you so much for all of your ideas and help with this, I am most grateful. Sincerely, Nadia Gale Histotechnologist Centre for Translational and Applied Genomics (CTAG) 3427-600 West 10th Avenue Vancouver, BC V5Z 4E6 tel 604-877-6000 ext 2426 fax 604-877-6089 ngale@bccancer.bc.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From caligirl004 <@t> hotmail.com Thu Jun 28 19:53:50 2007 From: caligirl004 <@t> hotmail.com (Victoria Socin) Date: Thu Jun 28 19:54:01 2007 Subject: [Histonet] Salary Info for North Carolina Message-ID: Hi! I'm relocating to North Carolina, and was wondering what the salary range is for a registered Histo. tech. in North Carolina. If anyone can give me some information on this, it would be greatly appreciated! Thanks, Vicki _________________________________________________________________ Picture this – share your photos and you could win big! http://www.GETREALPhotoContest.com?ocid=TXT_TAGHM&loc=us From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Jun 29 01:25:57 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Jun 29 01:26:06 2007 Subject: [Histonet] Re: Nuclear artefactI Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222E7DD@wahtntex2.waht.swest.nhs.uk> Your coupling is elegantly performed . Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Your job is a manifestation of your spirit in the physical world. You can pretend that this is not the case, basically ignore it, or you can consciously claim it. You get to choose. --Ric Giardina This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Jun 29 01:49:08 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Jun 29 01:49:17 2007 Subject: [Histonet] Iron Stain Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222E7E0@wahtntex2.waht.swest.nhs.uk> Am I mistaken or is the Prussian Blue and Perl's Iron stain the same thing? Can someone provide some insight on this? The Ferrocyanide reaction of M. Perls (Virchows Arch. Pathol. Anat., 39:42 1867)demonstrates Fe2+ as dark blue which I think is 'Turnbull's Blue'. Lillies 1964 Technic for the Prussian Blue and Turnbull's blue reaction for Fe2+ and Fe3+ demonstrates Fe3+ as dark Prussian Blue and Fe2+ as bark blue Turnbull's Blue. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Your job is a manifestation of your spirit in the physical world. You can pretend that this is not the case, basically ignore it, or you can consciously claim it. You get to choose. --Ric Giardina This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Jun 29 02:03:45 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Jun 29 02:03:54 2007 Subject: [Histonet] Nuclear artefact II Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222E7E5@wahtntex2.waht.swest.nhs.uk> Thought we had agreed a common cause for this? Poor fixation from either using a suboptimal fixative, or a fixative for a suboptimal period, followed by processing that may, or may not, cause artefacts. You say that you've varied the time of fixation (formalin) but have you varied the fixative? Cytologists rarely (I think) see this artefact in their preparations and I've certainly not seen it in LBC slides (either Cytyc or Sure Path) but I've seen in many Histology Labs in the UK. The difference? Cytology specimens are 'usually' better fixed (albeit with alcohol and acetic acid) and not processed. Have you tried 'Carnoy's' range of fixative for short periods on thin blocks? You have to try and do this scientifically as I get the feeling that the artefact may be caused by a variety of things but IMHO fixation (poor) is the main causative one. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Your job is a manifestation of your spirit in the physical world. You can pretend that this is not the case, basically ignore it, or you can consciously claim it. You get to choose. --Ric Giardina This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Jacqueline.Malam <@t> rli.mbht.nhs.uk Fri Jun 29 03:28:02 2007 From: Jacqueline.Malam <@t> rli.mbht.nhs.uk (Malam Jacqueline) Date: Fri Jun 29 03:31:45 2007 Subject: [Histonet] EGFR clone with Benchmark XT Message-ID: Has anyone any experience of the best EGFR clone to use with the Ventana Benchmark XT besides 3C6? Many thanks Jacqui malam Royal lancaster infirmary uk DISCLAIMER: This e-mail is confidential and privileged. If you are not the intended recipient please accept our apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform postmaster@rli.mbht.nhs.uk that this message has gone astray before deleting it. Comments or opinions expressed in this email are those of their respective contributors only. The views expressed do not represent the views of the Trust, its management or employees. University Hospitals of Morecambe Bay NHS Trust is not responsible and disclaims any and all liability for the content of comments written within.Thank you for your co-operation. From drkwolfe <@t> telus.net Fri Jun 29 04:14:53 2007 From: drkwolfe <@t> telus.net (Joseph Kapler) Date: Fri Jun 29 04:15:05 2007 Subject: [Histonet] BSc Specialization or BSc with Minors in Histotechnology, Etc., Message-ID: Good Morning Ladies and Gentlement (I heard the Number of true chiveralous gentlemen has been dwindling severely over the last 10 or 20 years... Time to raise the next generaltion of True Gentle Men.) The purposes of this posting is to ask a very important Question for your feedback (Not what you think I would want to hear, or what you think I should do) (I want to hear your suggestions). I am currently working within a Histology Lab here in the City, who will keep me gainfully employeed. The catch, I have to teach myself Histology and Zoology to get buy while I am waiting on classes. So, what would your answers be to my question. Remember, what would YOU do and why, not what you think I should do... The Question is: Being a Student entering into the BSc program, I am looking at whether I should just stay in the BSWc program as general sciences degree or should I go and speciallize somewhere? But Where? Or, would you take your BSc in Science and minor in anothe arts program. My personal opinion would be to generalize, and then find a way to major in Histotechnology, or someting else along those lines. However, I have to take into consideration that I may not be able to continue with specialization unless I return for anotehr goaround. THanks Y'all, and I look forward to hearing your responses on this. Joe "DarkWolfe" Kapler From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Jun 29 05:08:51 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Jun 29 05:09:00 2007 Subject: [Histonet] BSc Specialization or BSc with Minors in Histotechnology, Etc., Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222E7F3@wahtntex2.waht.swest.nhs.uk> My personal opinion would be to generalize, and then find a way to major in Histotechnology, or someting else along those lines. However, I have to take into consideration that I may not be able to continue with specialization unless I return for anotehr go around. Depends on what you want to do. If you aren't sure of your path, then generalise and then find a way as you say. If you're sure of your path then specialise as you say. If you want someone else to make your mind up for your then ask this Forum and end up in a mess. Have you asked your Mum? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Your job is a manifestation of your spirit in the physical world. You can pretend that this is not the case, basically ignore it, or you can consciously claim it. You get to choose. --Ric Giardina This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From oshel1pe <@t> cmich.edu Fri Jun 29 07:10:24 2007 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Fri Jun 29 07:10:41 2007 Subject: [Histonet] Repair of ultramicrotome In-Reply-To: <001001c7b9df$c1d5d350$0202a8c0@HPPav2> References: <001001c7b9df$c1d5d350$0202a8c0@HPPav2> Message-ID: Peggy, This is the same microtome as the RMC MT-5000. RMC bought the DuPont/Sorvall line in the middle of the MT-5000 production run. There were some minor changes, but nothing RMC can't handle. (RMC was then bought by Boeckler Instruments. They also distribute Bal-Tec.) http://www.baltec-rmc.com/cms/index.cfm/path/17933/26003/ This should get you to the service contact page. You can also call (520) 745-0001 -- ask for Dave Roberts, he runs the EM products group. I've always found RMC to be very helpful and good with their customer support. I've opened and worked on a RMC MT-5000. And gave up. It's not a great machine. If you can it would be better to buy one of their new PowerTomes. If you can't ... the problem isn't likely to be a computer glitch. Unless yours is very different, there isn't a computer, just mechanical parts and sensors, some integrated circuits, but no software or the like. Might be a fried IC at that ... Phil >Could someone recommend, or vendors reply to, my need for someone able to >repair a Sorvall Dupont MT 5000 ultramicrotome. > >The Visutrac and the action of the block arm to coordinate is way off. In >other words, instead of slowing down during the interval where the diamond >knife edge is, the block arm will: >- sometimes slow down, >- sometimes continue at the same speed, >- sometimes slow down in the area above or below the knife edge but go at >the fast speed at the knife edge, >and, to make it even more fun >- sometimes the block arm will reverse direction - go UP instead of >continuing down, but just right around the knife edge. > >Sort of fascinating to watch, as you never know what it is going to do. But >this makes it impossible to cut sections. > >We've tried turning the ultramicrotome on and off, and changed the Visutrac >setting, and altered the nanometer thickness (600 angstroms and 1 um) - >nothing seems to make a difference. I'm guessing it's a "computer glitch" in >the machine, and I'm not about it try to open up the ultramicrome to fix it >(coward, I know). > >If you could email it to my work address (pwenk@beaumont.edu), instead of >just replying to this email (home), I would get your reply faster. > >Thanks in advance. I'm hoping the Histonetters can come through for me on >this, as you have on other of my requests. Don't you just love this >community? > >Peggy A. Wenk, HTL(ASCP)SLS >William Beaumont Hospital >Royal Oak, MI 48073 -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 From Terry.Marshall <@t> rothgen.nhs.uk Fri Jun 29 07:42:15 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Jun 29 08:00:29 2007 Subject: [Histonet] Nuclear artefact II Message-ID: <5C0BED61F529364E86309CADEA63FEF25E6C57@TRFT-EX01.xRothGen.nhs.uk> Perhaps when you have the time, people might follow these 2 links to pictures showing bubbles in both a frozen section and a pleural fluid. Then explain how it is due to formalin fixation. Terry http://good-times.webshots.com/photo/2041628840033405579pfBiXf http://good-times.webshots.com/photo/2568678980033405579hWlUOc -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: 29 June 2007 01:20 To: 'Gale, Nadia'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Nuclear artefact II >From what I've read about nuclear bubbling, it all starts with >inadequate fixation. Anything else we do to the tissue just makes it worse. Nothing we can do will ever improve poorly fixed tissue. If there are not enough cross-links of formalin (or any other fixative) due to underfixation, then these few cross-links can be easily broken later on. This causes the proteins and DNA being easily moved around into new locations. So some of the things that can move the proteins and DNA are: - processing (going through alcohols and xylene will warp these under cross-linked proteins/DNA even more) - too much heat in processing (alcohols, xylene, paraffin) - too long of time in hot paraffin - too much heat during slide drying The reason we see this artifact mostly with formalin fixed tissue is two fold: 1) Formalin needs 1-2 days to completely fix 3 mm thick tissue sections. In other words, we need that amount of time to create enough cross-links so that nuclear bubbling and other protein deformities do not occur later with processing, heat, etc. 2) Formalin is a lousy nuclear fixative. Mercury and Zinc are much better fixatives for nuclei. And tend to fix faster than formalin. So B5, Zenker, Zinc-Formalin are less likely to show nuclear bubbling. However, the worst case of nuclear bubbling I ever saw (orphan annie eyes) was in bone marrow biopsies that were underfixed in zinc formalin (tech didn't want to wait the usual amount of time, so only let them fix about half the time required). So, increasing formalin fixative by one hour is NOT going to correct this problem. It's going to take several hours increase in time in formalin before some decrease in nuclear bubbling is seen. So leave the tissue on the processor in the formalin as long as possible at the beginning of the cycle, NOT in the hot paraffin at the end of the cycle. After that, watch the temperatures during processing (keeping at 37 degrees C would be better than 42 degrees, for example for the alcohols/xylene; keep paraffin as low as possible (2 degrees above melting point)). Keep the temperature on the slide dryer down too. 60 degrees C for 30 minutes will be better than 80 degrees C for 15 minutes. And tell the people grossing that they MUST cut thin sections (thickness of a nickle = 2 mm), and NOT overstuff the cassettes. These will not allow formalin to flow around the tissue. So the tissue won't get fixed! So no cross-links, so no protein/DNA stabilization. Ergo, another reason for nuclear bubbling! Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gale, Nadia Sent: Thursday, June 28, 2007 12:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Nuclear artefact II I've looked at everyone's suggestions/comments and am sad to report that I cannot get rid of the nuclear "bubble" artefact from our slides. I have tried - decreasing water bath temp - increasing/decreasing drying oven time before H&E staining - varying fixation times in formalin I am currently trying - decreasing drying oven temperature - changing the "wrap" that I use for the biopsies when grossing - looking at our wax times on the processor I agree with Bob that it's extremely frustrating that our community cannot seem to agree on a common cause for this (what I am now realizing) common artefact. I'll keep you posted with my results. Thank you so much for all of your ideas and help with this, I am most grateful. Sincerely, Nadia Gale Histotechnologist Centre for Translational and Applied Genomics (CTAG) 3427-600 West 10th Avenue Vancouver, BC V5Z 4E6 tel 604-877-6000 ext 2426 fax 604-877-6089 ngale@bccancer.bc.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Jun 29 08:05:14 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Jun 29 08:05:22 2007 Subject: [Histonet] Nuclear artefact II Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222E800@wahtntex2.waht.swest.nhs.uk> I thought she'd said inadequate fixation not just formalin fixation; could it be that the frozen section and pleural fluid were inadequately fixed? Or not fixed at all, which I suppose would prove her point, wouldn't it? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Your job is a manifestation of your spirit in the physical world. You can pretend that this is not the case, basically ignore it, or you can consciously claim it. You get to choose. --Ric Giardina This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Terry.Marshall <@t> rothgen.nhs.uk Fri Jun 29 08:51:43 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Jun 29 09:02:16 2007 Subject: [Histonet] Nuclear artefact II Message-ID: <5C0BED61F529364E86309CADEA63FEF25E6C5A@TRFT-EX01.xRothGen.nhs.uk> You're flapping desperately:-) Terry -----Original Message----- From: Kemlo Rogerson [mailto:Kemlo.Rogerson@waht.swest.nhs.uk] Sent: 29 June 2007 14:05 To: Marshall Terry Dr, Consultant Histopathologist; lpwenk@sbcglobal.net; Gale, Nadia; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Nuclear artefact II I thought she'd said inadequate fixation not just formalin fixation; could it be that the frozen section and pleural fluid were inadequately fixed? Or not fixed at all, which I suppose would prove her point, wouldn't it? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Your job is a manifestation of your spirit in the physical world. You can pretend that this is not the case, basically ignore it, or you can consciously claim it. You get to choose. --Ric Giardina This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From dlcowie <@t> prodigy.net Fri Jun 29 10:03:51 2007 From: dlcowie <@t> prodigy.net (Dawn Cowie) Date: Fri Jun 29 10:04:02 2007 Subject: [Histonet] Eosin on processor In-Reply-To: <001d01c7b9dc$754fdba0$d49eae18@yourxhtr8hvc4p> Message-ID: <383079.369.qm@web81002.mail.mud.yahoo.com> We also do this. It does not interfere at all with the H&E stain or subsequent IHC's. Yes, it is a life saver for small bx's. It makes it much easier to find them in the cassette when embedding and also when they are being cut - they show up very nicely in the block. You can see more easily if you are going deep enough for a good section, etc. DLC Joe Nocito wrote: Linda, my last lab put 25 mls of Eosin in the first 100% alcohol container on each of the tissue processors. It didn't interfere with the H&E staining, it did stain up the tissue processors, but wasn't a factor as to their operations. Since the lab processed mostly biopsies, the Eosin was a life saver, especially when it came to GI and prostate biopsies. Hope this helps. Joe ----- Original Message ----- From: "Linda Hines" To: Sent: Thursday, June 28, 2007 5:48 PM Subject: [Histonet] Eosin on processor >I would like to know which of the labs are using Eosin on their tissue >processor to mark the small specimens. Also if you have a procedure, if it >has interfered with your processing, and the staining of your H&E. I've >seen this before and liked it when working with the finite, and some of the >needle bx's. This is just one of my sudgestions for our lab. Thanx for >your input. > Linda > TRMC > > > --------------------------------- > It's here! Your new message! > Get new email alerts with the free Yahoo! Toolbar. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SecrestK <@t> wvuh.com Fri Jun 29 10:24:26 2007 From: SecrestK <@t> wvuh.com (Secrest, Kimberly) Date: Fri Jun 29 10:24:58 2007 Subject: [Histonet] Good references Message-ID: <7708E0A8E46BDC40B23113F97FB0FB23031DD047@nt-exchange1.wvuh.wvuhs.com> I'm looking to update our histology reference library (routine, special stains and IHC). Does anybody have any suggestions for good reference books? Is "Microscopy, Immunohistochemistry and Antigen Retrieval Methods: For Light and Electron Microscopy" by M.A. Hayat a good book? Thanks Kimberly Secrest HTL, QIHC Histology Technical Specialist West Virginia University Hospital From vsnider <@t> shrinenet.org Fri Jun 29 10:29:35 2007 From: vsnider <@t> shrinenet.org (Snider, Vivian Deanna) Date: Fri Jun 29 10:29:43 2007 Subject: [Histonet] QC sheets for routine cutting and H&E Staining Message-ID: <84BE46B37B314D409C5A17B7BAB022D60153389D@IDC-EX-VS01.shriners.cc> Does anybody have a standard QC sheet for tracking routine cutting and staining of tissue that they could fax or email me? I could make one in Excel, but quite frankly just do not have the time! Thanks in advance, Deanna Snider Lead Tech Shriners Hospital for Children Cincinnati, OH 513-872-6388 513-872-6299 fax CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients. If you are not the intended recipient, (or authorized to receive for the recipient) you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please destroy all copies of this communication and any attachments and contact the sender by reply e-mail or telephone (813) 281-0300. From LRaff <@t> lab.uropartners.com Fri Jun 29 12:26:23 2007 From: LRaff <@t> lab.uropartners.com (Lester Raff) Date: Fri Jun 29 12:26:29 2007 Subject: [Histonet] unsubscribe Message-ID: <5DA1CA5D0B98A84985B545A24423B822052FF1@UPLAB01.uplab.local> Unsubscribe for vacation Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 From dmccaig <@t> ckha.on.ca Fri Jun 29 12:32:13 2007 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Fri Jun 29 12:32:22 2007 Subject: [Histonet] McKesson Histology Message-ID: Is anyone using McKesson histology computer system in their lab? Diana McCaig Histology Lab Chatham Kent Health Alliance Chatham. Ontario N8A 5E1 519-352-6401 (6604) From LuckG <@t> empirehealth.org Fri Jun 29 12:40:01 2007 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Fri Jun 29 12:40:13 2007 Subject: [Histonet] training for non-certified HTs In-Reply-To: <467A2F49020000670000E8A4@gwgate.cscc.edu> Message-ID: <6BB8BC4519AAB844B174FC739A679BBCCEFE37@IRMEXCH01.irm.inhs.org> Hi Peggy, Thanks for the info this morning. Below I have attached my contact info to initiate our string of correspondences. Thanks, Greg Greg Luck, B.S., HT(ASCP) Anatomic Pathology Supervisor Deaconess Med Cntr 800 W. 5th Ave Spokane, WA 99204 Offc 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconessmc.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Peggy Mayo Sent: Thursday, June 21, 2007 4:57 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] training for non-certified HTs To all Supervisors and Managers, If you are interested in a formal training program to get your non-certified HTs eligible for certification, please visit my program website at: www.cscc.edu/histology. This is a 3 quarter (approximately 9 months) distance program with an integrated clinical experience. This experience can be fulfilled in approved laboratories (that meet our accreditation standards) and after a clinical affiliate agreement has been signed. The next class will begin Fall, 2008, with applications due May 1, 2008. If you are a supervisor/manager and are interested, you may contact me directly at this email or my phone # below. Thank you. Peggy Mayo Peggy Mayo M.Ed. MLT (ASCP) Multi-Competency Health Technology 614-287-2608 or 800-621-6407 ext. 2608 Fax 614-287-3854 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Robert.Lott <@t> TriadHospitals.com Fri Jun 29 13:22:47 2007 From: Robert.Lott <@t> TriadHospitals.com (Lott, Robert) Date: Fri Jun 29 13:22:56 2007 Subject: [Histonet] Contact info. for Winsome Garvey Message-ID: <518A08C53ED96D419F498D309E64A36A0CF3C0@CPRTEVS03.triadhospitals.net> Does anyone know how I can contact Winsome... ??? Robert L. Lott, HTL(ASCP) Manager, Anatomic Pathology LabFirst / Trinity Medical Center - formerly Montclair Baptist Medical Center 800 Montclair Road Birmingham, AL 35213 205-592-5388 phone 205-592-5646 fax robert.lott@triadhospitals.com From cindipqr <@t> wowway.com Fri Jun 29 14:27:55 2007 From: cindipqr <@t> wowway.com (cindipqr@wowway.com) Date: Fri Jun 29 14:28:07 2007 Subject: [Histonet] Iron Stain In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBF0222E7E0@wahtntex2.waht.swest.nhs.uk> References: <86ADE4EB583CE64799A9924684A0FBBF0222E7E0@wahtntex2.waht.swest.nhs.uk> Message-ID: <20070629191751.M92848@wowway.com> Does anybody have the Lillie's procedures they could send me? Thanks! Cindi Roberts HFH Detroit, Mi ---------- Original Message ----------- From: "Kemlo Rogerson" To: "Derek Papalegis" , Sent: Fri, 29 Jun 2007 07:49:08 +0100 Subject: RE: [Histonet] Iron Stain > Am I mistaken or is the Prussian Blue and Perl's Iron stain the same > > thing? Can someone provide some insight on this? > > The Ferrocyanide reaction of M. Perls (Virchows Arch. Pathol. Anat., > > 39:42 1867)demonstrates Fe2+ as dark blue which I think is > 'Turnbull's Blue'. > > Lillies 1964 Technic for the Prussian Blue and Turnbull's blue reaction > for Fe2+ and Fe3+ demonstrates Fe3+ as dark Prussian Blue and Fe2+ as > bark blue Turnbull's Blue. > > Kemlo Rogerson > > Pathology Manager > > DD 01934 647057 or extension 3311 > > Mob 07749 754194; Pager 07659 597107; > > Your job is a manifestation of your spirit in the physical world. You > can pretend that this is not the case, basically ignore it, or you > can consciously claim it. You get to choose. --Ric Giardina > > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance > on its contents: to do so is strictly prohibited and may be > unlawful. Please inform me that this message has gone astray before > deleting it. Thank you for your co-operation > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------- End of Original Message ------- From PHORGE1 <@t> Fairview.org Fri Jun 29 15:31:00 2007 From: PHORGE1 <@t> Fairview.org (Horge, Pamela J) Date: Fri Jun 29 15:31:10 2007 Subject: [Histonet] HTL Positions Message-ID: <2CFE87A91F5ACB4DB062CF69A861F8BD0333AB34@digsmxmbx03.Fairview.org> Histotechnologist University of Minnesota Medical Center, Fairview Minneapolis, Minnesota We have an opportunity for two full-time histotechnologists. One position concentrates on immunohistochemistry, the other on training/orientation and procedure writing. They also perform routine and specialized procedures in the Histopathology Laboratory, including in-situ hybridization, same-day rush processing, microwave processing, kidney biopsies, dermatology specimens and research. Qualified candidates must be HTL (ASCP) certified or eligible, experience preferred Are you are interested in working in a vibrant, nationally renowned teaching and research hospital? Do you want to perform rare and esoteric testing, and use state of the art equipment, utilizing many of the cutting edge technologies? Apply online at fairview.org Requisition # 07-19423 and 07-19419 Pamela Horge phorge1@fairview.org Histology Supervisor University of Minnesota Medical Center, Fairview Phone: 612-273-2884 Pager: 612-899-7036 Fax: 612-273-9124 From erweber <@t> maxhealth.com Fri Jun 29 15:47:03 2007 From: erweber <@t> maxhealth.com (Eric Weber) Date: Fri Jun 29 15:45:15 2007 Subject: [Histonet] Need Some Help Message-ID: <9D12D4EF30176D4F839EB47F3D0E8436015A5009@exbk2.maxhealth.com> I work for a staffing agency. Don't worry, I am not soliciting. For compliance reasons, all our employees need to take an exam in their field before they can begin work. Unfortunately, we currently do not have an exam for a histology tech. I do not want to lose creditability by continuing to ask my histology employees to take a MLT exam. Would anyone be interested in providing me a histology technician exam? Please contact me if you would be able to help. Thank you in advance. Eric Weber Recruitment Specialist Maxim Staffing Solutions Toll Free (866) 466-2974 Toll Free Fax (866) 466-2981 erweber@maxhealth.com From Asf2k3 <@t> aol.com Fri Jun 29 23:11:02 2007 From: Asf2k3 <@t> aol.com (Asf2k3@aol.com) Date: Fri Jun 29 23:11:11 2007 Subject: [Histonet] Re: Histonet Digest, Vol 43, Issue 38 Message-ID: 1. How long should tissue paraffin blocks should be soaked in ice before sectioning tissue with microtome? 2. How long should slides stay in oven to dry before they are put in automated stainer? Please let me know.... Thanks ************************************** See what's free at http://www.aol.com. From Asf2k3 <@t> aol.com Fri Jun 29 23:17:44 2007 From: Asf2k3 <@t> aol.com (Asf2k3@aol.com) Date: Fri Jun 29 23:17:52 2007 Subject: [Histonet] Re: Histonet Digest, Vol 43, Issue 35 Message-ID: What is the best way to not have bubbles when embedding tissue in mold. Well I know one way, tap the top cover, the bubble will go away, but this is too time consuming if you have 100 blocks to embed in 1 hr and half time frame. Does any one know what quicker way is possible? A ************************************** See what's free at http://www.aol.com. From talulahgosh <@t> gmail.com Fri Jun 29 23:35:20 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Jun 29 23:35:28 2007 Subject: [Histonet] Re: Histonet Digest, Vol 43, Issue 35 In-Reply-To: References: Message-ID: What are you embedding with? Using paraffin and sucrose/OCT, we just pour slowly and bubbles aren't created. You can also push them to the sides of the embedding mold with a needle or forceps; bubbles near the edge of the block shouldn't interrupt your sectioning because they'll be trimmed off. Emily -- penguins, spoons, and you--what's life like among the flightless? http://www.getafirstlife.com/ From talulahgosh <@t> gmail.com Fri Jun 29 23:45:16 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Jun 29 23:45:20 2007 Subject: [Histonet] Re: Histonet Digest, Vol 43, Issue 38 Message-ID: When we paraffin section stage 24 to 31 chick embryos (which are relatively small from what I know of most sectioning), we leave the blocks overnight at room temperature after embedding and until sectioning. Sometimes we place them at 4C until we section, but I've never noticed a difference between the two temperatures after processing. Because we are a research lab, we don't have the same deadlines as a pathology lab, so we leave our paraffin blocks around indefinitely--this may make a difference. After sectioning, though, we store our slides at 4C because this supposedly helps them keep better. We do not have an automated stainer, but we usually don't let slides dry when paraffin sectioned because they are already dry. If you're worried about sections falling off, you can heat them on a slide warmer at 37C for 15 minutes. This shouldn't be necessary though, since your slides don't have water in them from the paraffin processing. Emily -- penguins, spoons, and you--what's life like among the flightless? http://www.getafirstlife.com/ From talulahgosh <@t> gmail.com Fri Jun 29 23:47:34 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Jun 29 23:47:39 2007 Subject: [Histonet] reply to option Message-ID: Please, please change the default "reply to" option on the histonet list to reply to the list, not the individual. The questions I ask and answer may apply to the whole list, not just the person asking. Emily -- penguins, spoons, and you--what's life like among the flightless? http://www.getafirstlife.com/ From talulahgosh <@t> gmail.com Sat Jun 30 00:02:48 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Sat Jun 30 00:02:54 2007 Subject: [Histonet] Re: Histonet Digest, Vol 43, Issue 35 In-Reply-To: References: Message-ID: I just realized something that others may not do--our paraffin is melted in a vacuum oven, which decreases the amount of bubbles in the paraffin. Do you use one of these? This may be more valuable than I realize, since I've never used paraffin without a vacuum oven. To answer your other email, we leave our blocks at 4C anywhere from overnight to a month later. Again, I've never noticed a difference when sectioning cold paraffin blocks from room temperature blocks. Emily -- penguins, spoons, and you--what's life like among the flightless? http://www.getafirstlife.com/ From kemlo <@t> f2s.com Sat Jun 30 05:32:46 2007 From: kemlo <@t> f2s.com (kemlo) Date: Sat Jun 30 05:33:21 2007 Subject: [Histonet] Nuclear artefact II In-Reply-To: <5C0BED61F529364E86309CADEA63FEF25E6C5A@TRFT-EX01.xRothGen.nhs.uk> Message-ID: <4347E2B425634157822F9E8AAEADED6B@KemloPC> You're ignoring the question furtively:) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: 29 June 2007 14:52 To: Kemlo Rogerson; lpwenk@sbcglobal.net; Gale, Nadia; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Nuclear artefact II You're flapping desperately:-) Terry -----Original Message----- From: Kemlo Rogerson [mailto:Kemlo.Rogerson@waht.swest.nhs.uk] Sent: 29 June 2007 14:05 To: Marshall Terry Dr, Consultant Histopathologist; lpwenk@sbcglobal.net; Gale, Nadia; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Nuclear artefact II I thought she'd said inadequate fixation not just formalin fixation; could it be that the frozen section and pleural fluid were inadequately fixed? Or not fixed at all, which I suppose would prove her point, wouldn't it? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Your job is a manifestation of your spirit in the physical world. You can pretend that this is not the case, basically ignore it, or you can consciously claim it. You get to choose. --Ric Giardina This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sat Jun 30 11:57:34 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Jun 30 11:57:39 2007 Subject: [Histonet] Need Some Help In-Reply-To: <9D12D4EF30176D4F839EB47F3D0E8436015A5009@exbk2.maxhealth.com> Message-ID: <000c01c7bb37$c2a09e10$6501a8c0@Patsy> Eric, Are you not hiring ASCP certified histotechs? If you are they have already taken an exam. If they are not certified they should become so. I would not want anyone working for me who was not ASCP certified. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eric Weber Sent: Friday, June 29, 2007 2:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Need Some Help I work for a staffing agency. Don't worry, I am not soliciting. For compliance reasons, all our employees need to take an exam in their field before they can begin work. Unfortunately, we currently do not have an exam for a histology tech. I do not want to lose creditability by continuing to ask my histology employees to take a MLT exam. Would anyone be interested in providing me a histology technician exam? Please contact me if you would be able to help. Thank you in advance. Eric Weber Recruitment Specialist Maxim Staffing Solutions Toll Free (866) 466-2974 Toll Free Fax (866) 466-2981 erweber@maxhealth.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From drkwolfe <@t> telus.net Sat Jun 30 12:08:41 2007 From: drkwolfe <@t> telus.net (Joseph Kapler) Date: Sat Jun 30 12:08:47 2007 Subject: [Histonet] Re: Histonet Digest, Vol 43, Issue 38 In-Reply-To: Message-ID: Depending upon the quality of the paraffin used, most blocks can now be cut at room temperature with very little noticeable difference to the sectioner. However, I agree that some tissues section better when cooled. Therefore we either simply refridgerate the blocks before sectioning for 15 so minutes, or we use the cold plate from the paraffin blocker for a minute or two. For drying slides (I assume your referrence is for the waterbath and not for water in the paraffin and tissues), we gennerally leave the slides to dry for a couple of hours where possible. As with the earlier response given, we are small research/diagnostics lab and do everything manually. Once the slides have dried (or nearly dried) we bake the slides for 15 minutes (longer for damp slides, 30 minutes). This should be good for the automated system. J.Kapler -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Asf2k3@aol.com Sent: Friday, June 29, 2007 22:11 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 43, Issue 38 1. How long should tissue paraffin blocks should be soaked in ice before sectioning tissue with microtome? 2. How long should slides stay in oven to dry before they are put in automated stainer? Please let me know.... Thanks ************************************** See what's free at http://www.aol.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Sat Jun 30 15:06:10 2007 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sat Jun 30 15:06:22 2007 Subject: [Histonet] Need Some Help In-Reply-To: <9D12D4EF30176D4F839EB47F3D0E8436015A5009@exbk2.maxhealth.com> Message-ID: <000501c7bb52$1b8dd1e0$0202a8c0@HPPav2> Is this a certification exam? Then they need to take the HT or HTL exam from the ASCP Board of Registry. http://www.ascp.org/Certification/CertifyingExaminations/cert_procedures/def ault.aspx Is this a competency exam/assessment? I would suggest the Self-Assessment Booklets from the National Society of Histotechnology (NSH). There are a total of 14 books - 5 on non- staining - fixation, processing, sectioning, safety, laboratory operations (equipment, lab math, management, etc.), as well as 9 booklets on stains (theory/H&E/Giemsa, microorganisms, connective tissue, carbohydrates, etc.). Or, you can buy them all on 1 CD. http://www.nsh.org/organizations.php3?action=printContentItem&orgid=111&type ID=1157&itemID=18326 Pick and chose questions for your competency assessments/exams. Good idea for all histo labs that need to demonstrate competency of their histotechs, such as for CAP requirements. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eric Weber Sent: Friday, June 29, 2007 4:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Need Some Help I work for a staffing agency. Don't worry, I am not soliciting. For compliance reasons, all our employees need to take an exam in their field before they can begin work. Unfortunately, we currently do not have an exam for a histology tech. I do not want to lose creditability by continuing to ask my histology employees to take a MLT exam. Would anyone be interested in providing me a histology technician exam? Please contact me if you would be able to help. Thank you in advance. Eric Weber Recruitment Specialist Maxim Staffing Solutions Toll Free (866) 466-2974 Toll Free Fax (866) 466-2981 erweber@maxhealth.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sharon.osborn <@t> comcast.net Sat Jun 30 15:10:07 2007 From: sharon.osborn <@t> comcast.net (sharon.osborn@comcast.net) Date: Sat Jun 30 15:10:10 2007 Subject: [Histonet] Bubbles in embedding Message-ID: <063020072010.7025.4686B89E000D2E2000001B712213539653029D010D9C01D202019D0E089C@comcast.net> There is a technic that some techs discover/figure out with observation in doing these tasks.You may wish to try what I do: When filling the mold with the melted paraffin, place just enough to make a skin to hold the tissue in orientation. Place the tissue with quick chill then add more melted paraffin to the rim of the mold. Most bubbles occur when the paraffin is below the rim of the block when placing the plastic top on. Gently place the plastic top on the mold with paraffin and tissue. The paraffin immediately creates a connection (seal) with the plastic top. Now, add a thin layer of paraffin and place on the cold plate to partially set up (cool) then add the rest of the paraffin. Rarely do I have bubbles in the paraffin when I use this technic. It can be done quickly, succinctly and successfully with practice. My problem comes when the cold plate becomes full and there is no more room because the first molded blocks are not quite ready to be removed. Solution is to have another cold plate available. It also helps when the cold plates get cold enough and stay that way to begin with. Sharon Osborn LabVision ThermoFisher Scientific Fremont, CA Message: 9 Date: Sat, 30 Jun 2007 00:17:44 EDT From: Asf2k3@aol.com Subject: [Histonet] Re: Histonet Digest, Vol 43, Issue 35 To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" What is the best way to not have bubbles when embedding tissue in mold. Well I know one way, tap the top cover, the bubble will go away, but this is too time consuming if you have 100 blocks to embed in 1 hr and half time frame. Does any one know what quicker way is possible? A From Asf2k3 <@t> aol.com Sat Jun 30 18:37:04 2007 From: Asf2k3 <@t> aol.com (Asf2k3@aol.com) Date: Sat Jun 30 18:37:12 2007 Subject: [Histonet] Re: Histonet Digest, Vol 43, Issue 40 Message-ID: What is the best way to avoid having bubbles in mold when embedding tissue. We just use regular melted parrafin in embedding center. What I did is put parraffin in mold, then put in on cold plate to start freezing a bit, then put the tissue so it will adhere to mold, then put the cassette top on mold, and fill it up with parrafin. I do this step but still see bubbles forming. I tap the cassette top on mold to get rid of bubbles which works, but this step is not convenience if you have to embed quickly because I have to embed like 120 blocks in hr and half. A Please let me know. ************************************** See what's free at http://www.aol.com. From Asf2k3 <@t> aol.com Sat Jun 30 18:40:04 2007 From: Asf2k3 <@t> aol.com (Asf2k3@aol.com) Date: Sat Jun 30 18:40:10 2007 Subject: [Histonet] Re: Histonet Digest, Vol 43, Issue 40 Message-ID: When trimming blocks, do you have to face tissue all the way or just enough? A ************************************** See what's free at http://www.aol.com.