From LRaff <@t> lab.uropartners.com Sun Jul 1 08:03:42 2007 From: LRaff <@t> lab.uropartners.com (Lester Raff) Date: Sun Jul 1 08:03:46 2007 Subject: [Histonet] unsubscribe Message-ID: <5DA1CA5D0B98A84985B545A24423B822A642@UPLAB01.uplab.local> unsubscribe Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory ph: 708-486-0076 fax: 708-486-0080 From Histopatty <@t> aol.com Sun Jul 1 09:23:58 2007 From: Histopatty <@t> aol.com (Histopatty@aol.com) Date: Sun Jul 1 09:24:12 2007 Subject: [Histonet] Quality and Turn around, and what about floaters? Message-ID: I recently visited a certain company that claimed to have a solution for consistency in quality stain and an answer to the problem of carry over. The stainer stained slides individually thus eliminating carry over in the stain solution and insuring high quality staining for each slide since the solution was fresh each time. We did a comparison of our facility stained slides with recuts of the same slides stained on their system. There was a marked difference in the quality of the biopsies stained, (more subcellular details, such as villi, and brush boarders are visible) but on the larger specimens the difference was not so evident. I applaud the innovation of this company to bring H&E staining up to higher standards. However this still leaves me wondering if there is a way to individualize/optimize processing of specimens. Obviously the biopsies are easier to optimize because of their smaller size. But when it comes to processing larger specimens the optimization is lost due to many factors: type of tissue (fat, smooth muscle, etc.), thickness variations, and last but not least fixation time. In our facility we train resident pathologist, so that may be part of the problem, as well as the turn around time issues due to regulating organization and customer demands. I am wondering what other facilities do if anything to optimize specimen processing (which seems essential) in order to have consistency in the quality of all slides with a turn around time that is acceptable to all concerned. We currently have 3 routine processing schedules, one for biopies, routine specimens and larger fatty specimens. We utilize 5 processors with staggered starting times. Any insight or suggestion will be appreciated. Patty E. OUMC ************************************** See what's free at http://www.aol.com. From kemlo <@t> f2s.com Sun Jul 1 10:05:44 2007 From: kemlo <@t> f2s.com (kemlo) Date: Sun Jul 1 10:04:05 2007 Subject: [Histonet] Re: Histonet Digest, Vol 43, Issue 40 In-Reply-To: Message-ID: <000c01c7bbf1$4cd999b0$0202a8c0@KEMLOS> Flame it with a bunsen. What? Mr Kemlo Rogerson MSc DMS MIBiol CBiol FIBMS Pathology Services Manager Weston General Hospital North Somerset -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Asf2k3@aol.com Sent: 01 July 2007 00:37 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 43, Issue 40 What is the best way to avoid having bubbles in mold when embedding tissue. We just use regular melted parrafin in embedding center. What I did is put parraffin in mold, then put in on cold plate to start freezing a bit, then put the tissue so it will adhere to mold, then put the cassette top on mold, and fill it up with parrafin. I do this step but still see bubbles forming. I tap the cassette top on mold to get rid of bubbles which works, but this step is not convenience if you have to embed quickly because I have to embed like 120 blocks in hr and half. A Please let me know. ************************************** See what's free at http://www.aol.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kemlo <@t> f2s.com Sun Jul 1 10:06:10 2007 From: kemlo <@t> f2s.com (kemlo) Date: Sun Jul 1 10:04:29 2007 Subject: [Histonet] Re: Histonet Digest, Vol 43, Issue 40 In-Reply-To: Message-ID: <000d01c7bbf1$5c1522f0$0202a8c0@KEMLOS> Just enough. Mr Kemlo Rogerson MSc DMS MIBiol CBiol FIBMS Pathology Services Manager Weston General Hospital North Somerset -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Asf2k3@aol.com Sent: 01 July 2007 00:40 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 43, Issue 40 When trimming blocks, do you have to face tissue all the way or just enough? A ************************************** See what's free at http://www.aol.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kimtournear <@t> yahoo.com Sun Jul 1 11:59:26 2007 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Sun Jul 1 11:59:30 2007 Subject: [Histonet] Need Some Help Message-ID: <732986.83758.qm@web50602.mail.re2.yahoo.com> Eric, I have to agree with Patsy...although, just because a person has passd the exam and becomes certified (lots of book smart people out there), doesn't mean they are good (hands on application) at what they do....I guess it would have to be up to your client as to whether or not they prefer that the tech is certified or not....they might only care that the tech can do the job and do it well....good luck Kim Tournear, HT (ASCP), QIHC ( ASCP) Specialists in Dermatology Histology/Mohs Supervisor Tucson, AZ --------------------------------- Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. From Malcolm.McCallum <@t> tamut.edu Sun Jul 1 12:04:02 2007 From: Malcolm.McCallum <@t> tamut.edu (Malcolm McCallum) Date: Sun Jul 1 12:37:08 2007 Subject: [Histonet] google alternative to ISI impact ratings looks good References: <000d01c7bbf1$5c1522f0$0202a8c0@KEMLOS> Message-ID: Publish or Perish is a new citation rating program based on google scholar that seems to give more sensible results. I think we are all now aware of the Journal IMpact Ratings game. ISI pretty much has control of this market and excludes piles of journals from their analysis. To be included in ISI a journal cannot be over specialized, it cannot skip an issue or be late or slow, it must be older than 2 years, it must be cited in current ISI ranked journals, it should not cite more than 13% of its own articles during the rated interval, its editorial board ideally has an international component, all articles must be in eglish, and there are a number of other factors that can disqualify a journal. The corporate journals have a clear advantage here and they even encourage authors to cite papers from other journals from their corporation in order to inflate ratings (yes, its been published!). Publish or perish (http://www.harzing.com/resources.htm#/pop.htm) is an alternative to ISI, its free, and it uses the Google Scholar database to formulate rankings for journals, authors, and even articles. Granted that scholar isn't complete, but recent analyses reveal it is now more complete than ISI since ISI only includes cites from ISI journals, scholarly books, and ignores non-english publications among other factors. Scholar includes all of these sources, however, it misses some old articles, although this is changing with the expansion of BioOne, JStore, etc. So, from a citation analysis point of view, P&P is probably (in my opinion) more accurate than ISI because it now covers more citations. I did a few searches in the sciences. PP gies a ton of stats, here are 3. h-index = similar to the ISI rating, but not limited to last two years (slightly modified ISI rating) g-index = similar to h-index, slightly diffent formula AWCR = age-weighted citation rate, ave # of citations for a paper adjusted for the age of each paper. Of course, new journals (under 3 yr old) will rate low no matter what. First I list some generalized journals then a mess of herp journals by PP ranking and give their ISI rating in parentheses. If the journal is not rated it will say NR in the parentheses and if it is too new or defunct, it will have new or defunct in the paretheses. It is very interesting how some journals have decent ranking on PP and others are high on ISI. The rating by PP makes more sense to me. IF anyone thinks otherwise, let me know! Generalized journals: Science (30.93): h-index = 601, g-index = 917, AWCR = 70495 Nature(29.27): h-index = 593, g-index = 949, AWCR = 72135 BioScience (4.71): h-index = 114, g-index = 173, AWCR = 2904 Ecology (ISI = 4.51): h-index = 172, g-index = 255, AWCR = 7780 American Naturalist (ISI = 4.46): h-index = 53, g-index = 73, AWCR = 2186 American Midland Naturalist (ISI = 0.77): h-index = 49, g-index = 71, AWCR = 814 PloS Biology (ISI = 14.7): h-index = 24, g-index = 45, AWCR = 605 Southwestern Naturalist (ISI = 0.30): h-index = 23, g-index = 31, AWCR = 318 Southeastern Naturalist (ISI = 0.33):h-index = 7, g-index = 9, AWCR = 2.39 HERP JOURNALS (ranked by AWCR): Copeia (ISI = 0.974): h-index = 51, g-index = 77, AWCR = 1153 Journal of Herpetology (ISI = 0.817): h-index = 33, g-index = 43, AWCR = 841 Herpetologica (ISI = 0.922): h-index = 37, g-index = 51, AWCR = 668 Amphibia-Reptilia (ISI = 0.547): h-index = 20, g-index = 28, AWCR = 331 Herpetological Review (NR): h-index = 14, g-index = 17, AWCR = 216 Chelonian Conservation Biology (NR): h-index = 18, g-index = 25, AWCR = 187 Herpetological Journal(ISI = 0.71): h-index = 15, g-index = 19, AWCR = 152 Alytes (NR): h-index = h-index = 11, g-index = 16, AWCR = 78.09 Herpetological Natural History (defunct): h-index = 9, g-index = 12, AWCR = 32.68 Russian Journal of Herpetology (NR): h-index = 8, g-index = 10, AWCR = 32.34 Applied Herpetology (NR): h-index = 4, g-index = 5, AWCR = 19.45 Cuadernos de herpetologia (NR): h-index = 6, g-index = 7, AWCR = 14.27 Amphibian and Reptile Conservation (NR): h-index = 6, g-index = 8, AWCR = 12.86 British Journal of Herpetology (NR): h-index = 6, g-index = 11, AWCR = 7.34 Acta Herpetologica (new): h-index = 4, g-index = 5, AWCR = 5.78 Contemporary Herpetology (NR): h-index = 4, g-index = 6, AWCR = 5.19 Journal of Kansas Herpetology (NR): h-index = 1, g-index = 1, AWCR = 1.80 Manouria (NR): h-index = 2, g-index = 2, AWCR = 1.59 Brazilian Journal of Herpetology (NR): h-index = 1, g-index = 1, AWCR = 0.06. South American Journal of Herpetology (new) : h-index = 1, g-index = 1, AWCR = 0 Malcolm L. McCallum Assistant Professor Department of Biological Sciences Texas A&M University Texarkana 2600 Robison Rd. Texarkana, TX 75501 O: 1-903-223-3134 H: 1-903-791-3843 Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html VISIT HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org A New Journal Published in Partnership with Partners in Amphibian and Reptile Conservation and the World Congress of Herpetology. Summer Teaching Schedule & Office Hours: Monday-Thursday: Statistics (Math 453) 4-5:50 pm Office Hours: 3-4 pm "Set high standards for yourself, but don't judge others by those standards," - Gary Emmert (University of Memphis) ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of kemlo Sent: Sun 7/1/2007 10:06 AM To: Asf2k3@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Histonet Digest, Vol 43, Issue 40 Just enough. Mr Kemlo Rogerson MSc DMS MIBiol CBiol FIBMS Pathology Services Manager Weston General Hospital North Somerset -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Asf2k3@aol.com Sent: 01 July 2007 00:40 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 43, Issue 40 When trimming blocks, do you have to face tissue all the way or just enough? A ************************************** See what's free at http://www.aol.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From adesupo2002 <@t> hotmail.com Sun Jul 1 12:56:39 2007 From: adesupo2002 <@t> hotmail.com (ADESUPO ADESUYI) Date: Sun Jul 1 12:56:44 2007 Subject: [Histonet] Pipettes Calibration Message-ID: Hi, Pls we are trying to calibrate our pipettes, so i will appreciate it, if you guys could suggest some ideas to me, on how to go about doing this. You guys are the best. Ade. _________________________________________________________________ Play free games, earn tickets, get cool prizes! Join Live Search Club.? http://club.live.com/home.aspx?icid=CLUB_wlmailtextlink From jnocito <@t> satx.rr.com Sun Jul 1 16:00:40 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Sun Jul 1 16:00:45 2007 Subject: [Histonet] Quality and Turn around, and what about floaters? References: Message-ID: <000c01c7bc22$e23829f0$d49eae18@yourxhtr8hvc4p> we did the same thing. We put GI bxs on one program, prostates on another, skins on another and everything else goes on one long processing program. We have staining program for prostate bxs and everything else gets the routine program. I'm curious as to which company this is. Joe ----- Original Message ----- From: To: Sent: Sunday, July 01, 2007 9:23 AM Subject: [Histonet] Quality and Turn around, and what about floaters? >I recently visited a certain company that claimed to have a solution for > consistency in quality stain and an answer to the problem of carry over. > The > stainer stained slides individually thus eliminating carry over in the > stain > solution and insuring high quality staining for each slide since the > solution was > fresh each time. We did a comparison of our facility stained slides with > recuts of the same slides stained on their system. There was a marked > difference > in the quality of the biopsies stained, (more subcellular details, such as > villi, and brush boarders are visible) but on the larger specimens the > difference > was not so evident. I applaud the innovation of this company to bring H&E > staining up to higher standards. However this still leaves me wondering > if there > is a way to individualize/optimize processing of specimens. Obviously the > biopsies are easier to optimize because of their smaller size. But when > it > comes to processing larger specimens the optimization is lost due to many > factors: > type of tissue (fat, smooth muscle, etc.), thickness variations, and last > but > not least fixation time. In our facility we train resident pathologist, > so > that may be part of the problem, as well as the turn around time issues > due to > regulating organization and customer demands. I am wondering what other > facilities do if anything to optimize specimen processing (which seems > essential) > in order to have consistency in the quality of all slides with a turn > around > time that is acceptable to all concerned. We currently have 3 routine > processing schedules, one for biopies, routine specimens and larger fatty > specimens. > We utilize 5 processors with staggered starting times. Any insight or > suggestion will be appreciated. > > Patty E. > OUMC > > > > ************************************** See what's free at > http://www.aol.com. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Sun Jul 1 23:51:34 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Sun Jul 1 23:51:40 2007 Subject: [Histonet] Pipettes Calibration In-Reply-To: References: Message-ID: We have representatives of companies calibrate our pipetmen on site to save on the cost of shipping. I don't know if that is usual for most businesses though. Otherwise, we send our pipetmen to Rainin (the manufacturer) for calibration. Keep in mind that there are newer pipetmen that can be calibrated by anyone (ISC Bioexpress sent us a description of how to calibrate their pipetman ourselves), and replacing something like the O-ring is much less expensive if you do it yourself. Rainin has a booklet on replacing simple items like the O-ring, and then recalibrating your pipetman if you need it. You could also use the density of pure water to calibrate your pipetmen, but that may not be exact enough for your procedures. Emily -- penguins, spoons, and you--what's life like among the flightless? http://www.getafirstlife.com/ From talulahgosh <@t> gmail.com Mon Jul 2 00:24:01 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Mon Jul 2 00:24:07 2007 Subject: [Histonet] Re: Histonet Digest, Vol 43, Issue 40 In-Reply-To: <000d01c7bbf1$5c1522f0$0202a8c0@KEMLOS> References: <000d01c7bbf1$5c1522f0$0202a8c0@KEMLOS> Message-ID: It sounds silly, but I think you might be pouring the paraffin too fast. Try slowing down when you get to that point. Also, you could pour the paraffin down a stirring rod into the mold. If you don't have a vacuum oven for your paraffin, that could also be your problem. I know I said this in the last email, but we've never had bubble problems, and we embed very quickly. The vacuum may make the bubble difference. In fact, the only reason we use the vacuum oven (as opposed to anything that would melt the paraffin) is because it gets rid of bubbles. Emily -- penguins, spoons, and you--what's life like among the flightless? http://www.getafirstlife.com/ From joyce.christopher <@t> bayercropscience.com Mon Jul 2 06:31:02 2007 From: joyce.christopher <@t> bayercropscience.com (Joyce Christopher) Date: Mon Jul 2 06:31:17 2007 Subject: [Histonet] helicobactor, campylobacter stain Message-ID: What is the latest, quickest way to stain for helicobactor, campylobacter? Joyce Christopher, HT (ASCP) Histology Coordinator Bayer CropScience LP 17745 S Metcalf Stilwell, KS 66085-9104 913-433-5205/5244 Fax# 913-433-5125 joyce.christopher@bayercropscience.com _______________________________________________________________________________________________ The information contained in this e-mail is for the exclusive use of the intended recipient(s) and may be confidential, proprietary, and/or legally privileged. Inadvertent disclosure of this message does not constitute a waiver of any privilege. If you receive this message in error, please do not directly or indirectly use, print, copy, forward, or disclose any part of this message. Please also delete this e-mail and all copies and notify the sender. Thank you. For alternate languages please go to http://bayerdisclaimer.bayerweb.com _______________________________________________________________________________________________ From Terry.Marshall <@t> rothgen.nhs.uk Mon Jul 2 06:47:35 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Mon Jul 2 06:52:30 2007 Subject: [Histonet] Nuclear artefact II Message-ID: <5C0BED61F529364E86309CADEA63FEF25E6C67@TRFT-EX01.xRothGen.nhs.uk> OK. The FS was doused in aceto-alcohol as per usual. The pleural fluid was a cytospin (how I now hate that device) fixed in alcohol in our normal way. (It is as you observe no doubt, air dried at the edge from which I took the photo, but that's because the bubbles show better there). Now - there's the basis of a separate thread - how can you tell whether a smear is fixed well or not? What is "fixed well"? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kemlo Sent: 30 June 2007 11:33 To: Marshall Terry Dr, Consultant Histopathologist; 'Kemlo Rogerson'; lpwenk@sbcglobal.net; 'Gale, Nadia'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Nuclear artefact II You're ignoring the question furtively:) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: 29 June 2007 14:52 To: Kemlo Rogerson; lpwenk@sbcglobal.net; Gale, Nadia; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Nuclear artefact II You're flapping desperately:-) Terry -----Original Message----- From: Kemlo Rogerson [mailto:Kemlo.Rogerson@waht.swest.nhs.uk] Sent: 29 June 2007 14:05 To: Marshall Terry Dr, Consultant Histopathologist; lpwenk@sbcglobal.net; Gale, Nadia; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Nuclear artefact II I thought she'd said inadequate fixation not just formalin fixation; could it be that the frozen section and pleural fluid were inadequately fixed? Or not fixed at all, which I suppose would prove her point, wouldn't it? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Your job is a manifestation of your spirit in the physical world. You can pretend that this is not the case, basically ignore it, or you can consciously claim it. You get to choose. --Ric Giardina This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Mon Jul 2 07:22:19 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon Jul 2 07:22:31 2007 Subject: [Histonet] Re: Histonet Digest, Vol 43, Issue 38 In-Reply-To: Message-ID: <001401c7bca3$a55523e0$d00f7ca5@lurie.northwestern.edu> ALL, We are a research lab as well as human pathology and we "cook" our slides for a minimum of an hour (to overnight) in a 60 degree oven prior to IHC and special stains. For H&E they air dry until we put them on an automated stainer in which the dryer/oven is set for 10 minutes and 65 degrees. Unstained slides are stored at 4C and stained inside of 30 days (usually IHC) to avoid loss of antigenicity. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joseph Kapler Sent: Saturday, June 30, 2007 12:09 PM To: Asf2k3@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Histonet Digest, Vol 43, Issue 38 Depending upon the quality of the paraffin used, most blocks can now be cut at room temperature with very little noticeable difference to the sectioner. However, I agree that some tissues section better when cooled. Therefore we either simply refridgerate the blocks before sectioning for 15 so minutes, or we use the cold plate from the paraffin blocker for a minute or two. For drying slides (I assume your referrence is for the waterbath and not for water in the paraffin and tissues), we gennerally leave the slides to dry for a couple of hours where possible. As with the earlier response given, we are small research/diagnostics lab and do everything manually. Once the slides have dried (or nearly dried) we bake the slides for 15 minutes (longer for damp slides, 30 minutes). This should be good for the automated system. J.Kapler -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Asf2k3@aol.com Sent: Friday, June 29, 2007 22:11 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 43, Issue 38 1. How long should tissue paraffin blocks should be soaked in ice before sectioning tissue with microtome? 2. How long should slides stay in oven to dry before they are put in automated stainer? Please let me know.... Thanks ************************************** See what's free at http://www.aol.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Mon Jul 2 07:27:17 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon Jul 2 07:27:29 2007 Subject: [Histonet] Need Some Help In-Reply-To: <732986.83758.qm@web50602.mail.re2.yahoo.com> Message-ID: <001601c7bca4$56ee8880$d00f7ca5@lurie.northwestern.edu> All, When I interviewed for my first job out of histo school, I was sat at a microtome and had to cut sections. Seems like a good test to me!!! I've done it to others, especially if they are new in the field or registry eligible. Since ASCP/BOR has seen fit to remove the practical this is the next best way. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Tournear Sent: Sunday, July 01, 2007 11:59 AM To: Histonet Subject: RE: [Histonet] Need Some Help Eric, I have to agree with Patsy...although, just because a person has passd the exam and becomes certified (lots of book smart people out there), doesn't mean they are good (hands on application) at what they do....I guess it would have to be up to your client as to whether or not they prefer that the tech is certified or not....they might only care that the tech can do the job and do it well....good luck Kim Tournear, HT (ASCP), QIHC ( ASCP) Specialists in Dermatology Histology/Mohs Supervisor Tucson, AZ --------------------------------- Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Mon Jul 2 07:48:26 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Jul 2 07:48:33 2007 Subject: [Histonet] Nuclear artefact II Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222E81A@wahtntex2.waht.swest.nhs.uk> OK. The FS was doused in aceto-alcohol as per usual. The pleural fluid was a cytospin (how I now hate that device) fixed in alcohol in our normal way. (It is as you observe no doubt, air dried at the edge from which I took the photo, but that's because the bubbles show better there). Now - there's the basis of a separate thread - how can you tell whether a smear is fixed well or not? What is "fixed well"? Terry So it dried before it was fixed? My point exactly and that's why the bubbles show better!!! "Fixed well" I assume means that the fixative has either attached itself completely to all those things it ought to attach itself to, altered the tertiary protein structures sufficiently to trap all the substances that are able to be trapped and rendered the matrix of the tissue's proteins sufficiently robust to withstand processing and exposed all those sites needing exposing to facilitate staining then I assume it is fixed optimally. Cytospins are, IMHO, the Devils tool and introduces artefacts and loses cells as you suggest; LBC is the way forward and I assume that means Cytyc? Has SurePath cracked the LBC world for Non-Gynae yet? As a recovering Cell Path Manager I'm losing touch, but oddly Pharmacies and the therapies are fun too. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Your job is a manifestation of your spirit in the physical world. You can pretend that this is not the case, basically ignore it, or you can consciously claim it. You get to choose. --Ric Giardina This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From cbass <@t> bidmc.harvard.edu Mon Jul 2 08:52:19 2007 From: cbass <@t> bidmc.harvard.edu (cbass@bidmc.harvard.edu) Date: Mon Jul 2 08:52:32 2007 Subject: [Histonet] What camera options are available for a Motic Inverted AE30? Message-ID: <4B5700BE37215344A81185F131A848C261EB81@EVS6.its.caregroup.org> Hey guys, I am trying to find a solution for taking photos with a Motic inverted microscope (AE30). As far as I can tell, there are no photo tubes. I believe this is a binocular version (the trinocular would have a photo tube). Are there any options? There is a $300 digital camera add-on that attaches to the eyepiece, but this seems a bit awkward, and I don't want to add a computer to the microscope. Any cheap third party options, maybe something that will allow a real digital camera which can be easily put on and taken off? We're doing neurite extension assays, so we need some way to photograph the cells and count the extensions. If there is another reasonable way (some sort of quantitative kit) I'd love to hear it. Thanks, Caroline From Terry.Marshall <@t> rothgen.nhs.uk Mon Jul 2 08:35:34 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Mon Jul 2 08:53:28 2007 Subject: [Histonet] Nuclear artefact II Message-ID: <5C0BED61F529364E86309CADEA63FEF25E6C6A@TRFT-EX01.xRothGen.nhs.uk> "So it dried before it was fixed? My point exactly and that's why the bubbles show better!!!" Eh? To what do you refer when you say..."that's why the bubbles show better."? The drying? If so, well yes, that's why I took the photo in that area. To what do you refer when you say...."My point exactly.."? That it dried before it was fixed? If so, I do not recall you ever having made a point about drying before fixation. Please remind me of the remark. Your short note on good fixation is fine, but is pure theory, and does not address the problem of how we can tell whether fixation has been "good" or not. Cytologists often call air drying poor fixation, but this is utter nonsense. Pathologists sometimes call autolysis poor fixation, but this too is pure nonsense. Has anybody else bothered to look at the photos on this mightily important issue? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo Rogerson Sent: 02 July 2007 13:48 To: Marshall Terry Dr, Consultant Histopathologist; kemlo; lpwenk@sbcglobal.net; Gale, Nadia; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Nuclear artefact II OK. The FS was doused in aceto-alcohol as per usual. The pleural fluid was a cytospin (how I now hate that device) fixed in alcohol in our normal way. (It is as you observe no doubt, air dried at the edge from which I took the photo, but that's because the bubbles show better there). Now - there's the basis of a separate thread - how can you tell whether a smear is fixed well or not? What is "fixed well"? Terry So it dried before it was fixed? My point exactly and that's why the bubbles show better!!! "Fixed well" I assume means that the fixative has either attached itself completely to all those things it ought to attach itself to, altered the tertiary protein structures sufficiently to trap all the substances that are able to be trapped and rendered the matrix of the tissue's proteins sufficiently robust to withstand processing and exposed all those sites needing exposing to facilitate staining then I assume it is fixed optimally. Cytospins are, IMHO, the Devils tool and introduces artefacts and loses cells as you suggest; LBC is the way forward and I assume that means Cytyc? Has SurePath cracked the LBC world for Non-Gynae yet? As a recovering Cell Path Manager I'm losing touch, but oddly Pharmacies and the therapies are fun too. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Your job is a manifestation of your spirit in the physical world. You can pretend that this is not the case, basically ignore it, or you can consciously claim it. You get to choose. --Ric Giardina This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Mon Jul 2 09:12:40 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Jul 2 09:12:46 2007 Subject: [Histonet] Nuclear artefact II Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222E820@wahtntex2.waht.swest.nhs.uk> "So it dried before it was fixed? My point exactly and that's why the bubbles show better!!!" Eh? To what do you refer when you say..."that's why the bubbles show better."? The drying? If so, well yes, that's why I took the photo in that area. To what do you refer when you say...."My point exactly.."? That it dried before it was fixed? If so, I do not recall you ever having made a point about drying before fixation. Please remind me of the remark. Your short note on good fixation is fine, but is pure theory, and does not address the problem of how we can tell whether fixation has been "good" or not. Cytologists often call air drying poor fixation, but this is utter nonsense. Pathologists sometimes call autolysis poor fixation, but this too is pure nonsense. Has anybody else bothered to look at the photos on this mightily important issue? Terry This is getting hard to follow........ The bubbles show better because the cell was not fixed immediately, but air dried. I concede that air drying is a form of fixation, but depending on how quickly (or not) it dries, then the fixation is optimal or not. Slow drying will allow some of the autolytic changes to happen and maybe the bubbles. My point was that it (the bubbles) was due to poor fixation and that's why the they (the bubbles) were more evident. I've looked at the photos and they remind me of Blackpool. I'm surprised that the theory of fixation, pure that it may be, is not acceptable (for example, I accept Einstein's Theory of Relativity without ever reaching the speed of light but I am putting on weight); if you have an alternate theory then feel free to share it with me. Air drying is fixation but if you wanted it not to be air dried but alcohol fixed then it still is good fixation (albeit not prompt enough) but poor IMHO preservation (the results are artefactual). Although if the time to air dry is protracted then it is both artefactual and WAS poorly fixed IMHO; you eventually fixed something that was not. Does that make sense? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Peace is not the absence of conflict but the presence of creative alternatives for responding to conflict. --Dorothy Thompson This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From michael.owen <@t> fda.hhs.gov Mon Jul 2 10:21:10 2007 From: michael.owen <@t> fda.hhs.gov (Owen, Michael P) Date: Mon Jul 2 10:21:33 2007 Subject: [Histonet] Pipettes Calibration In-Reply-To: Message-ID: <449E51C6DA0AD840B44F57C7A6EB07BF0160A56D@FMD3VS022.fda.gov> Dear Ade, My laboratory is accredited to ISO 17025. It follows the requirements established by A2LA. The facility, like other FDA Field Laboratories, also has to follow requirements by AOAC. AOAC previously required calibration of micropipettors on a quarterly basis. In order to save money, my laboratory decided to have cGMP-compatible service every six months. In between these service calls I would use a photometric system to perform quick calibration checks on the instruments. Due to environmental variables and lack of specialized equipment, a photometric system was used for these quick checks instead of gravimetric methods. Note gravimetric calibration requires a moderate amount of skill, therefore I left it to experts. AOAC and A2LA now specify micropipettors to be calibrated every six months. I have found in my experience the instruments need service, especially O-ring replacement, every six months therefore I have full service and calibration every six months performed by a contractor. In between service calls if an instrument fails I send it to Rainin in California. My laboratory uses Calibrate, Inc. to have its micropipettors calibrated every six months. The company has local employees who come to the laboratory and perform services on-site. The photometric system I mentioned is from Artel, Inc. The disadvantage of the system is the cost of the reagents, however the system is excellent for training and for facilities that need to check their micropipettors frequently. Calibrate, Inc. http://www.pipetpeople.com Artel USA, Inc. http://www.artel-usa.com If you have further questions, you can respond to the list or e-mail me. Sincerely, Michael FDA PRL-NW Micropipettor Monitor Michael P. Owen, Regulatory Microbiologist U.S. FDA Pacific Regional Lab Northwest 22201 23rd Drive SE Bothell, WA 98021-4421 Phone: 425-483-4865 E-Mail: michael.owen@fda.hhs.gov From pmarcum <@t> vet.upenn.edu Mon Jul 2 11:20:05 2007 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Mon Jul 2 11:20:10 2007 Subject: [Histonet] Region II Fall Symposium in Delaware This Year Message-ID: <6.2.5.6.2.20070702120028.01c8a8e0@vet.upenn.edu> Below is the draft copy of the program. We can not complete all full speaker titles at this time. The topics are correct for each speaker and the times. The full abstracts and program will be out in later this month and posted on each states web site. The cost per day is $80.00 or $40.00 per half day. Students and retirees will pay half price with proper identification. We will ask for registration as soon as possible as there will be an on-site registration penalty and you may also send in your registration with "funds pending" if your institution policy is slow and may not be ready before the meeting. As the meeting is being held at the Delaware Technical and Community College we have a list of area hotels at the end of this e-mail. Laboratories with multiple attendees should sent in all registration at the same time for a discount. This will apply to laboratories with 2 or more people attending on any combination of days. We are offering the chance for anyone wishing to only come to see the Exhibits ONLY a fee of $10.00 per day. This will not include any classes or lunch. Please contact me or Michelle Hart at MHart@Christianacare.org for further information Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu Region II Fall Symposium, September 6th, 7th, 8th 2007 Delaware Technical Community College-Stanton, DE Region II states are: Delaware, Pennsylvania, New Jersey, Maryland, and Virginia WE WELCOME ALL STATES IN OUR SURROUNDING AREA TO COME AND JOIN US!! Can?t get to Denver for the NSH Symposium and Convention yet still need CEU?s? Join us for the Region II Fall Symposium in Delaware! Thursday Seminars - will be geared toward management topics. 8:30 AM to 11:30 AM CPT Coding-How Do I Code This One? Bonnie Whitaker 3 hrs CAP Inspection-What?s New and Required Now Charlie Dorner 3 hrs 1:00 PM to 4:30 PM Lean ? Organizing Your Life and Lab Donna Montegue 3 hrs 1:00 PM to 2:30 PM Reducing Immunohistochemistry Expenses Joe Myers 1.5 hrs 3:00 PM to 4:30 PM To Be Announced Friday Seminars 8:30 AM to 12:00 PM Beginning IHC (new to the field or refresher) Bonnie Whitaker 3 hrs 8:30 AM to 10:00 AM Delaware Coroner Cases Dr. Callery 1.5 hrs Brain Diseases and Identification by Histology Dr. Crain 1.5 hrs Sentenial Nodes Dr. Mary Iococca 1.5 hrs 10:30 AM to 12:00 PM Tumor Classification Dr. Gary Witkin 1.5 hrs Deconvolution Microscopy Dr. Robert Akins 1.5 hrs Immunofluorescent for Kidney Biopsies Dr. Robert Garola 1.5 hrs 1:00 PM to 4:30 PM Microwave Processing Connie Wavrin 3 hrs Molecular Biology AbrahAM Joseph 3 hrs 1:00 PM to 2:30 PM FNA Cytology Osman Ouattara 1.5 hrs Round Table to Discuss Options in Histology-Several Speakers will discuss other options For careers in histology, such as Pharmaceutical Industry, Veterinary & Research or Industry Options for Sales and Technical Training 1.5 hrs 3:00 PM to 4:30 PM Bog People of Northern Europe Sandra Olson PhD 1.5 hrs ISH, FISH, PCR Mitch Gore 1.5hrs Saturday Seminars 8:30 AM to 4:30 PM QIHC Preparation Ethel Macrea 6 hrs HT Readiness Linda Foster-Brown 6 hrs 8:30 AM to 12:00 PM IHC-Locating and Validating New Antibodies And IHC Reagents Charles Dorner 3 hrs Ergonomics Jan Minchew 3 hrs 8:30 AM to 10:00 AM Interesting Case ? Can You Figure It Out? David Brenner 1.5 hrs Forensic Entomology Carolyn & Vincent D?AMico 1.5 hrs 10:30 AM to 12:00 PM Interesting Cases in Veterinary Pathology Dr. Perry Habecker 1.5 hrs Flow Cytometry Brenda Rabeno 1.5 hrs 1:00 PM to 4:30 PM IHC for Animal Histology (24 limit-wet) Zahra Naser 3 hrs Safety for Histology Sylvia Casey 3 hrs Grossing for Histology Connie Wavrin 3 hrs 1:00 PM to 2:30 PM Prions and How to Handle Them in Your Lab Dr. Michael Kanzer 1.5 hrs 3:00 PM to 4:30 PM Lab Math and Chemistry Donna Montegue 1.5 hrs All hotels are within approximately 1 mile of Delaware Technical and Community College. As we are not having the meeting in a hotel attendees are free to pick the best hotel to meet their needs. Here are the listings for the hotels in the area of DTCC Stanton CAMpus for the Region II in September: Courtyard by Marriot - 48 Geoffrey Dr - 302-456-3800 - This is the only hotel with a reserved block of rooms for the Region II Meeting. The rooms must be booked by August 9th to get a lower room rate. Christiana Hilton - 100 Continental Dr - 302-454-1500 Days Inn-900 - Churchmans Rd - 302-368-2400 Country Inn and Suites - 1024 Old Churchmans Rd - 302-2666-6400 Fairfield Inn by Marriot - 65 Geoffrey Dr - 302-292-1500 Red Roof Inns - 415 Stanton-Christiana Rd - 302-292-2870 Homestead Studio Suites Hotel - 333 Continental Dr - 302-283-0800 From rchiovetti <@t> yahoo.com Mon Jul 2 11:29:42 2007 From: rchiovetti <@t> yahoo.com (Robert Chiovetti) Date: Mon Jul 2 11:29:48 2007 Subject: [Histonet] What camera options are available for a Motic Inverted AE30? Message-ID: <382153.80108.qm@web58901.mail.re1.yahoo.com> Caroline, If your Motic scope doesn't have a trinocular head on it, you only have two options to attach a camera: 1. Buy a trinocular head. 2. Put a camera on the eyepiece or in the eyepiece tube. As far as attaching a camera, and you don't want to go with the MotiCam, I recommend you talk to Bobby Martin at Martin Microscope (www.martinmicroscope.com). Bobby makes several adapters that can mate just about any camera to any scope, either through an eyepiece tube or at a photo port on a trinocular head. You can take a look at the adapters he offers for consumer and "prosumer" cameras and then shop on the Internet for the camera, I suppose. If you want to do some kind of counting and quantitation of the neurite extensions, but you don't want to connect a computer to the scope, that will definitely be a problem. You could capture and store your images on a consumer camera w/ a memory card in it, then transfer the memory card to a card reader on a computer somewhere and copy the images onto the computer. That brings up the issue of the software you're going to use for the counting and quantitation. One way is cheap, everything else is going to cost you $$. I've not tried exactly what you have in mind, but it's possible that ImageJ might do it. ImageJ was developed at the National Institutes of Health using our tax dollars, so the software is free. It's not the most user-friendly package, but you can't beat the price. You can get started at: If you have the money and you want tech support, and you like talking to a real person who can help you with the imaging and analysis, then consider one of the commercial packages that's available, such as PAX-it (www.paxit.com) Hope this helps! Cheers, Bob Robert (Bob) Chiovetti, Ph.D. Southwest Precision Instruments Arizona's Microscopy Resource 132 North Elster Drive Tucson, AZ 85710-3212 Tel./Fax 520-546-4986 Member, Arizona Small Business Association www.swpinet.com (www.asba.com) ----- Original Message ---- From: "cbass@bidmc.harvard.edu" To: histonet@lists.utsouthwestern.edu Sent: Monday, July 2, 2007 6:52:19 AM Subject: [Histonet] What camera options are available for a Motic Inverted AE30? Hey guys, I am trying to find a solution for taking photos with a Motic inverted microscope (AE30). As far as I can tell, there are no photo tubes. I believe this is a binocular version (the trinocular would have a photo tube). Are there any options? There is a $300 digital camera add-on that attaches to the eyepiece, but this seems a bit awkward, and I don't want to add a computer to the microscope. Any cheap third party options, maybe something that will allow a real digital camera which can be easily put on and taken off? We're doing neurite extension assays, so we need some way to photograph the cells and count the extensions. If there is another reasonable way (some sort of quantitative kit) I'd love to hear it. Thanks, Caroline _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Get the free Yahoo! toolbar and rest assured with the added security of spyware protection. http://new.toolbar.yahoo.com/toolbar/features/norton/index.php From pruegg <@t> ihctech.net Mon Jul 2 12:21:07 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Jul 2 12:21:11 2007 Subject: [Histonet] Need Some Help In-Reply-To: <000501c7bb52$1b8dd1e0$0202a8c0@HPPav2> Message-ID: <005501c7bccd$61b44470$6501a8c0@Patsy> Great ideas Peggy. I know at one time Kathy Davis was working on a comprehensive competency program, did that become something one can get from NSH? I remember that it did not include IHC and our IHC Resource Group committee was trying to put something like this together for IHC. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Saturday, June 30, 2007 2:06 PM To: 'Eric Weber'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Need Some Help Is this a certification exam? Then they need to take the HT or HTL exam from the ASCP Board of Registry. http://www.ascp.org/Certification/CertifyingExaminations/cert_procedures/def ault.aspx Is this a competency exam/assessment? I would suggest the Self-Assessment Booklets from the National Society of Histotechnology (NSH). There are a total of 14 books - 5 on non- staining - fixation, processing, sectioning, safety, laboratory operations (equipment, lab math, management, etc.), as well as 9 booklets on stains (theory/H&E/Giemsa, microorganisms, connective tissue, carbohydrates, etc.). Or, you can buy them all on 1 CD. http://www.nsh.org/organizations.php3?action=printContentItem&orgid=111&type ID=1157&itemID=18326 Pick and chose questions for your competency assessments/exams. Good idea for all histo labs that need to demonstrate competency of their histotechs, such as for CAP requirements. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eric Weber Sent: Friday, June 29, 2007 4:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Need Some Help I work for a staffing agency. Don't worry, I am not soliciting. For compliance reasons, all our employees need to take an exam in their field before they can begin work. Unfortunately, we currently do not have an exam for a histology tech. I do not want to lose creditability by continuing to ask my histology employees to take a MLT exam. Would anyone be interested in providing me a histology technician exam? Please contact me if you would be able to help. Thank you in advance. Eric Weber Recruitment Specialist Maxim Staffing Solutions Toll Free (866) 466-2974 Toll Free Fax (866) 466-2981 erweber@maxhealth.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gamal.akabani <@t> gmail.com Mon Jul 2 12:27:14 2007 From: gamal.akabani <@t> gmail.com (Gamal Akabani) Date: Mon Jul 2 12:27:22 2007 Subject: [Histonet] Differences between sucrose and formalin Message-ID: <5ADF2749-677E-479B-940B-68AF362EE73F@gmail.com> Hi all, I would like to know what are the difference between sucrose and formalin fixation. I am trying to carry out some immunohistochemitrsy and autoradiography. Thank you in advanced Gamal From stamptrain <@t> yahoo.com Mon Jul 2 12:34:59 2007 From: stamptrain <@t> yahoo.com (Roger Moretz) Date: Mon Jul 2 12:35:05 2007 Subject: [Histonet] Differences between sucrose and formalin In-Reply-To: <5ADF2749-677E-479B-940B-68AF362EE73F@gmail.com> Message-ID: <25538.30379.qm@web55801.mail.re3.yahoo.com> Well, to my knowledge, sucrose is not a fixative (I know about the reports on honey, just nothing on sucrose). Formalin contains formaldehyde, a fixative. Sucrose is normally used to replace water for better tissue preservation during freezing when flash freezing is not done. Never heard of it as a fixative, though. I'm open to information to that event.... Roger Moretz, Ph.D. Dept of Toxicology (1 more day) Boehringer Ingelheim Pharmaceuticals, Inc. Ridgefield, CT (then I retire!!!!) --- Gamal Akabani wrote: > Hi all, > > I would like to know what are the difference between > sucrose and > formalin fixation. > I am trying to carry out some immunohistochemitrsy > and autoradiography. > > Thank you in advanced > > Gamal > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ____________________________________________________________________________________ Be a better Globetrotter. Get better travel answers from someone who knows. Yahoo! Answers - Check it out. http://answers.yahoo.com/dir/?link=list&sid=396545469 From mcauliff <@t> umdnj.edu Mon Jul 2 12:37:18 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Mon Jul 2 12:37:44 2007 Subject: [Histonet] Differences between sucrose and formalin In-Reply-To: <5ADF2749-677E-479B-940B-68AF362EE73F@gmail.com> References: <5ADF2749-677E-479B-940B-68AF362EE73F@gmail.com> Message-ID: <468937CE.3010107@umdnj.edu> Sucrose is NOT a fixative, formalin is. One -5% sucrose is sometimes added to formalin fixatives for osmotic effects. Geoff Gamal Akabani wrote: > Hi all, > > I would like to know what are the difference between sucrose and > formalin fixation. > I am trying to carry out some immunohistochemitrsy and autoradiography. > > Thank you in advanced > > Gamal > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From jb <@t> Dal.Ca Mon Jul 2 14:52:54 2007 From: jb <@t> Dal.Ca (Joanna Borowska) Date: Mon Jul 2 14:53:01 2007 Subject: [Histonet] copper localization Message-ID: <20070702165254.0tnj3kv7h3j4g0w4@my2.dal.ca> Hello everyone, Does anyone know of a reliable copper stain (histological or fluorescent) for brain, especially insect (Drosophila) brain? There can be paraffin or cryostat sections and intact whole brain (especially the last). Thanks in advance, Joanna Joanna Borowska, PhD Department of Psychology Life Sciences Centre, Dalhousie University 1355 Oxford Street Halifax, Nova Scotia Canada B3H 4J1 lab: (001) - 902 - 494 - 3746 fax: (001) - 902 - 494 - 6585 http://flylab.psychology.dal.ca/joanna06.html From shroffsm <@t> vcu.edu Mon Jul 2 15:14:53 2007 From: shroffsm <@t> vcu.edu (Seema Shroff) Date: Mon Jul 2 15:15:02 2007 Subject: [Histonet] Ki-67 marker for mouse CNS Message-ID: Hey, I'm looking to stain mouse 4% paraformaldehyde perfused, spinal cord frozen sections with the mitosis marker Ki-67. Does anyone know of a reliable one that works well in the mouse CNS and if you could please give me the company and catalogue number? Thank you, Seema Shroff Graduate Student Anatomy and Neurobiology Virginia Commonwealth University. From dcojita <@t> tampabay.rr.com Mon Jul 2 17:06:16 2007 From: dcojita <@t> tampabay.rr.com (dcojita@tampabay.rr.com) Date: Mon Jul 2 17:08:21 2007 Subject: [Histonet] Need Some Help In-Reply-To: <001601c7bca4$56ee8880$d00f7ca5@lurie.northwestern.edu> Message-ID: I agree. I have a "practical" test for new applicants as well. I have them cut 5 or so blocks, observe their cutting technique, how they choose to label the slide, mount the section, etc. Its great insight on how they will perform. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Monday, July 02, 2007 8:27 AM To: 'Kim Tournear'; 'Histonet' Subject: RE: [Histonet] Need Some Help All, When I interviewed for my first job out of histo school, I was sat at a microtome and had to cut sections. Seems like a good test to me!!! I've done it to others, especially if they are new in the field or registry eligible. Since ASCP/BOR has seen fit to remove the practical this is the next best way. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Tournear Sent: Sunday, July 01, 2007 11:59 AM To: Histonet Subject: RE: [Histonet] Need Some Help Eric, I have to agree with Patsy...although, just because a person has passd the exam and becomes certified (lots of book smart people out there), doesn't mean they are good (hands on application) at what they do....I guess it would have to be up to your client as to whether or not they prefer that the tech is certified or not....they might only care that the tech can do the job and do it well....good luck Kim Tournear, HT (ASCP), QIHC ( ASCP) Specialists in Dermatology Histology/Mohs Supervisor Tucson, AZ --------------------------------- Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Mon Jul 2 17:18:31 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon Jul 2 17:18:41 2007 Subject: [Histonet] Need Some Help In-Reply-To: Message-ID: Is there any concern about the liability of non-employee cutting themselves during this test? Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu Sent by: histonet-bounces@lists.utsouthwestern.edu 07/02/2007 03:06 PM To "'Bernice Frederick'" , "'Kim Tournear'" , "'Histonet'" cc Subject RE: [Histonet] Need Some Help I agree. I have a "practical" test for new applicants as well. I have them cut 5 or so blocks, observe their cutting technique, how they choose to label the slide, mount the section, etc. Its great insight on how they will perform. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Monday, July 02, 2007 8:27 AM To: 'Kim Tournear'; 'Histonet' Subject: RE: [Histonet] Need Some Help All, When I interviewed for my first job out of histo school, I was sat at a microtome and had to cut sections. Seems like a good test to me!!! I've done it to others, especially if they are new in the field or registry eligible. Since ASCP/BOR has seen fit to remove the practical this is the next best way. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Tournear Sent: Sunday, July 01, 2007 11:59 AM To: Histonet Subject: RE: [Histonet] Need Some Help Eric, I have to agree with Patsy...although, just because a person has passd the exam and becomes certified (lots of book smart people out there), doesn't mean they are good (hands on application) at what they do....I guess it would have to be up to your client as to whether or not they prefer that the tech is certified or not....they might only care that the tech can do the job and do it well....good luck Kim Tournear, HT (ASCP), QIHC ( ASCP) Specialists in Dermatology Histology/Mohs Supervisor Tucson, AZ --------------------------------- Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Mon Jul 2 18:01:24 2007 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Jul 2 18:01:37 2007 Subject: [Histonet] helicobactor, campylobacter stain Message-ID: We use a 1% giemsa in dist water for 30min Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Christopher Sent: Monday, 2 July 2007 9:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] helicobactor, campylobacter stain What is the latest, quickest way to stain for helicobactor, campylobacter? Joyce Christopher, HT (ASCP) Histology Coordinator Bayer CropScience LP 17745 S Metcalf Stilwell, KS 66085-9104 913-433-5205/5244 Fax# 913-433-5125 joyce.christopher@bayercropscience.com ________________________________________________________________________ _______________________ The information contained in this e-mail is for the exclusive use of the intended recipient(s) and may be confidential, proprietary, and/or legally privileged. Inadvertent disclosure of this message does not constitute a waiver of any privilege. If you receive this message in error, please do not directly or indirectly use, print, copy, forward, or disclose any part of this message. Please also delete this e-mail and all copies and notify the sender. Thank you. For alternate languages please go to http://bayerdisclaimer.bayerweb.com ________________________________________________________________________ _______________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From mtarango <@t> nvcancer.org Mon Jul 2 18:14:01 2007 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Mon Jul 2 18:14:11 2007 Subject: [Histonet] Ki-67 marker for mouse CNS In-Reply-To: Message-ID: <5AEC610C1CE02945BD63A395BA763EDE011B6D1A@NVCIEXCH02.NVCI.org> I've used dako's M7249 monoclonal rat anti-mouse ki67 followed with a biotinylated goat anti-rat with ABC detection. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Seema Shroff Sent: Monday, July 02, 2007 1:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ki-67 marker for mouse CNS Hey, I'm looking to stain mouse 4% paraformaldehyde perfused, spinal cord frozen sections with the mitosis marker Ki-67. Does anyone know of a reliable one that works well in the mouse CNS and if you could please give me the company and catalogue number? Thank you, Seema Shroff Graduate Student Anatomy and Neurobiology Virginia Commonwealth University. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From JWEEMS <@t> sjha.org Mon Jul 2 19:50:37 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Mon Jul 2 19:51:03 2007 Subject: [Histonet] Need Some Help In-Reply-To: Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FF9D5D@sjhaexc02.sjha.org> We don't do it for that reason... Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital of Atlanta 404-851-7376 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Monday, July 02, 2007 6:19 PM To: dcojita@tampabay.rr.com Cc: 'Histonet'; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Need Some Help Is there any concern about the liability of non-employee cutting themselves during this test? Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu Sent by: histonet-bounces@lists.utsouthwestern.edu 07/02/2007 03:06 PM To "'Bernice Frederick'" , "'Kim Tournear'" , "'Histonet'" cc Subject RE: [Histonet] Need Some Help I agree. I have a "practical" test for new applicants as well. I have them cut 5 or so blocks, observe their cutting technique, how they choose to label the slide, mount the section, etc. Its great insight on how they will perform. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Monday, July 02, 2007 8:27 AM To: 'Kim Tournear'; 'Histonet' Subject: RE: [Histonet] Need Some Help All, When I interviewed for my first job out of histo school, I was sat at a microtome and had to cut sections. Seems like a good test to me!!! I've done it to others, especially if they are new in the field or registry eligible. Since ASCP/BOR has seen fit to remove the practical this is the next best way. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Tournear Sent: Sunday, July 01, 2007 11:59 AM To: Histonet Subject: RE: [Histonet] Need Some Help Eric, I have to agree with Patsy...although, just because a person has passd the exam and becomes certified (lots of book smart people out there), doesn't mean they are good (hands on application) at what they do....I guess it would have to be up to your client as to whether or not they prefer that the tech is certified or not....they might only care that the tech can do the job and do it well....good luck Kim Tournear, HT (ASCP), QIHC ( ASCP) Specialists in Dermatology Histology/Mohs Supervisor Tucson, AZ --------------------------------- Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From zumbor <@t> email.cs.nsw.gov.au Mon Jul 2 20:09:54 2007 From: zumbor <@t> email.cs.nsw.gov.au (Rosalba) Date: Mon Jul 2 20:15:06 2007 Subject: [Histonet] (no subject) Message-ID: <01C7BD62.AFF3E5B0.zumbor@email.cs.nsw.gov.au> Hi All Just wondering if anyone has used any of the following antibodies on formalin fixed paraffin blocks. Wnt-1 Fgf-3 Fgf-10 Thanks Rosalba Zumbo Supervisor Histology Dept Department of Forensic Medicine 42-50 Parramatta Rd Glebe NSW 2037 Australia PH: 61 2 85847842 FAX: 61 2 95664573 zumbor@email.cs.nsw.gov.au "This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please destroy it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of the Sydney South West Area Health Service." From sarah <@t> als.co.nz Mon Jul 2 22:00:35 2007 From: sarah <@t> als.co.nz (Sarah O'Bryan) Date: Mon Jul 2 20:58:44 2007 Subject: [Histonet] ALK Protein - what do you use and how does it compare? Message-ID: <03568ED922E71549B7C3D55569A8C089127E2E@dchq.als.local> Hello all, I am interested in information on ALK Protein antibodies. Who's do you use, and how do you find it? Thanks in advance, Sarah O'Bryan Technical Specialist ALS NZ Ltd From amosbrooks <@t> gmail.com Mon Jul 2 22:38:04 2007 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Mon Jul 2 22:38:11 2007 Subject: [Histonet] Pipette Caliberation Message-ID: <582736990707022038j634fb2d6n9e0038891a72fc8b@mail.gmail.com> Ade, The easy way is to send them to a company to have them caliberated. Ranin does a great job of this. The hard way is to calibrate them yourself. Got time on your hands? Good, Use 3 pipette intervals at 25%, 50% and 75% of the pipette's volume. Set a weigh boat on a very sensitive electronic scale (which should also be calibrated professionally). Draw deionized H2O 10 times for each pipette at each interval. This also validates your drawing technique and consistency. (DON'T FUDGE THE DATA). Enter this data into Excel or some such spread sheet program. Now the fun part. Remember high school & college statistics and all that number crunching. The spread sheet can do it all for you, but if you don't know how the numbers are arrived at you won't know if the statistics formulae are right. You really should get at least the average for each interval, and the standard deviation at a pre-determined confidence interval (at the very least 95%). I really recommend doing this at least once for the truly geeky of us. This really justifies sending it to Ranin to business offices considering how long it can take. The upshot is that the results are beautiful with graphs and all. CAP totally leaves you alone if you can talk statistics. Usually scares the hell out of them. All the best, Amos Brooks From Kemlo.Rogerson <@t> waht.swest.nhs.uk Tue Jul 3 01:55:22 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Jul 3 01:55:30 2007 Subject: [Histonet] Differences between sucrose and formalin Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222E827@wahtntex2.waht.swest.nhs.uk> Ice cold Sucrose is used for 30 min prior to formalin in a 30% solution in order to prevent intrafixation diffusion or disruption of cell organelles. The formalin solution comprises of formalin 10ml, calcium acetate monohydrate 2g, sucrose 30g, make up to 100 ml with dist water. Fix for 18 hrs at 2 to 5 degrees C. Wash for 4 hours BEFORE at 20 to 30 degrees C to remove sucrose before processing or the block won't cut. Sucrose is also used with gum acacia in enzyme preservation. The sucrose must therefore be acting as a 'fixative' assume a lot like putting fruit in high strength sugar as a preservative; or maybe that's it, it preserves prior to fixation. Maybe it stops the formation of bubbles too (g). Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Peace is not the absence of conflict but the presence of creative alternatives for responding to conflict. --Dorothy Thompson This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From b-frederick <@t> northwestern.edu Tue Jul 3 07:24:19 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Tue Jul 3 07:24:31 2007 Subject: [Histonet] Need Some Help In-Reply-To: Message-ID: <001e01c7bd6d$1429ff10$d00f7ca5@lurie.northwestern.edu> If you've been training you should be wise enough to NOT cut yourself. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: Jennifer MacDonald [mailto:JMacDonald@mtsac.edu] Sent: Monday, July 02, 2007 5:19 PM To: dcojita@tampabay.rr.com Cc: 'Bernice Frederick'; 'Histonet'; histonet-bounces@lists.utsouthwestern.edu; 'Kim Tournear' Subject: RE: [Histonet] Need Some Help Is there any concern about the liability of non-employee cutting themselves during this test? Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu Sent by: histonet-bounces@lists.utsouthwestern.edu 07/02/2007 03:06 PM To "'Bernice Frederick'" , "'Kim Tournear'" , "'Histonet'" cc Subject RE: [Histonet] Need Some Help I agree. I have a "practical" test for new applicants as well. I have them cut 5 or so blocks, observe their cutting technique, how they choose to label the slide, mount the section, etc. Its great insight on how they will perform. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Monday, July 02, 2007 8:27 AM To: 'Kim Tournear'; 'Histonet' Subject: RE: [Histonet] Need Some Help All, When I interviewed for my first job out of histo school, I was sat at a microtome and had to cut sections. Seems like a good test to me!!! I've done it to others, especially if they are new in the field or registry eligible. Since ASCP/BOR has seen fit to remove the practical this is the next best way. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Tournear Sent: Sunday, July 01, 2007 11:59 AM To: Histonet Subject: RE: [Histonet] Need Some Help Eric, I have to agree with Patsy...although, just because a person has passd the exam and becomes certified (lots of book smart people out there), doesn't mean they are good (hands on application) at what they do....I guess it would have to be up to your client as to whether or not they prefer that the tech is certified or not....they might only care that the tech can do the job and do it well....good luck Kim Tournear, HT (ASCP), QIHC ( ASCP) Specialists in Dermatology Histology/Mohs Supervisor Tucson, AZ --------------------------------- Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LINDA.MARGRAF <@t> childrens.com Tue Jul 3 07:57:10 2007 From: LINDA.MARGRAF <@t> childrens.com (LINDA MARGRAF) Date: Tue Jul 3 07:57:32 2007 Subject: [Histonet] histologist position Medical College of Wisconsin Message-ID: <468A0156020000DA0000ED6D@CNET3.CHILDRENS.COM> Histonetters: Here is a message I was asked to post. Please contact them (not me) if you are interested. Thanks, Linda M (Histonet administrator) RESEARCH TECHNICIAN/HISTOLOGY The CRI Histology and Imaging Core at the Medical College of Wisconsin is seeking a full time Research Technician/Histology Technologist. Duties will include preparation of microscopic slides from human and animal tissue, primarily for research, including fixation, paraffin and frozen-embedding, and frozen and paraffin sectioning; assistance with quality control and client interactions; histochemical, immunohistochemical, immunofluorescence and in situ hybridization staining; maintenance and calibration of complex imaging systems (confocal, multi-photon, laser capture); help with training of core clients in use of imaging equipment and associated software systems; assistance in programming of processors, immunostainers, and other instrumentation; assistance with performance of widefield and confocal microscopic imaging, photomicroscopy, and image analysis. Skills: Excellent verbal and written communication skills are required, as are the ability to learn complex tasks, fine motor and organizational skills, basic mathematical and reasoning skills, proficiency with computers, and competence in the knowledge and skills required to perform his or her responsibilities. Requirements: Bachelors degree in chemistry, biology or related field required. Associate*s degree with strong relevant experience is acceptable. Three years of relevant general laboratory experience required. Histology Technician or Technologist Certification desired, but not required. Experience with imaging systems is strongly preferred. Apply online or send resume to: Medical College of Wisconsin Attn: Employment Office 8701 Watertown Plank Road Milwaukee, WI 53226 Jobline: 414-456-8193 careers@mcw.edu http://www.mcw.edu/hr EOE/AA M/F/D/V From mpence <@t> grhs.net Tue Jul 3 08:01:36 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Jul 3 08:01:42 2007 Subject: [Histonet] Need Some Help In-Reply-To: <001e01c7bd6d$1429ff10$d00f7ca5@lurie.northwestern.edu> Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C64E@IS-E2K3.grhs.net> But the liability is still there.....isn't it? Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Tuesday, July 03, 2007 7:24 AM To: 'Jennifer MacDonald'; dcojita@tampabay.rr.com Cc: 'Histonet'; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Need Some Help If you've been training you should be wise enough to NOT cut yourself. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: Jennifer MacDonald [mailto:JMacDonald@mtsac.edu] Sent: Monday, July 02, 2007 5:19 PM To: dcojita@tampabay.rr.com Cc: 'Bernice Frederick'; 'Histonet'; histonet-bounces@lists.utsouthwestern.edu; 'Kim Tournear' Subject: RE: [Histonet] Need Some Help Is there any concern about the liability of non-employee cutting themselves during this test? Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu Sent by: histonet-bounces@lists.utsouthwestern.edu 07/02/2007 03:06 PM To "'Bernice Frederick'" , "'Kim Tournear'" , "'Histonet'" cc Subject RE: [Histonet] Need Some Help I agree. I have a "practical" test for new applicants as well. I have them cut 5 or so blocks, observe their cutting technique, how they choose to label the slide, mount the section, etc. Its great insight on how they will perform. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Monday, July 02, 2007 8:27 AM To: 'Kim Tournear'; 'Histonet' Subject: RE: [Histonet] Need Some Help All, When I interviewed for my first job out of histo school, I was sat at a microtome and had to cut sections. Seems like a good test to me!!! I've done it to others, especially if they are new in the field or registry eligible. Since ASCP/BOR has seen fit to remove the practical this is the next best way. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Tournear Sent: Sunday, July 01, 2007 11:59 AM To: Histonet Subject: RE: [Histonet] Need Some Help Eric, I have to agree with Patsy...although, just because a person has passd the exam and becomes certified (lots of book smart people out there), doesn't mean they are good (hands on application) at what they do....I guess it would have to be up to your client as to whether or not they prefer that the tech is certified or not....they might only care that the tech can do the job and do it well....good luck Kim Tournear, HT (ASCP), QIHC ( ASCP) Specialists in Dermatology Histology/Mohs Supervisor Tucson, AZ --------------------------------- Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Tue Jul 3 08:07:55 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Tue Jul 3 08:08:06 2007 Subject: [Histonet] Need Some Help In-Reply-To: <661949901A768E4F9CC16D8AF8F2838CA1C64E@IS-E2K3.grhs.net> References: <001e01c7bd6d$1429ff10$d00f7ca5@lurie.northwestern.edu> <661949901A768E4F9CC16D8AF8F2838CA1C64E@IS-E2K3.grhs.net> Message-ID: but being wise does not prevent one from having accidents, look what happened to the Three Wise Men!. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: 03 July 2007 14:02 To: Bernice Frederick; Jennifer MacDonald; dcojita@tampabay.rr.com Cc: Histonet; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Need Some Help But the liability is still there.....isn't it? Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Tuesday, July 03, 2007 7:24 AM To: 'Jennifer MacDonald'; dcojita@tampabay.rr.com Cc: 'Histonet'; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Need Some Help If you've been training you should be wise enough to NOT cut yourself. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: Jennifer MacDonald [mailto:JMacDonald@mtsac.edu] Sent: Monday, July 02, 2007 5:19 PM To: dcojita@tampabay.rr.com Cc: 'Bernice Frederick'; 'Histonet'; histonet-bounces@lists.utsouthwestern.edu; 'Kim Tournear' Subject: RE: [Histonet] Need Some Help Is there any concern about the liability of non-employee cutting themselves during this test? Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu Sent by: histonet-bounces@lists.utsouthwestern.edu 07/02/2007 03:06 PM To "'Bernice Frederick'" , "'Kim Tournear'" , "'Histonet'" cc Subject RE: [Histonet] Need Some Help I agree. I have a "practical" test for new applicants as well. I have them cut 5 or so blocks, observe their cutting technique, how they choose to label the slide, mount the section, etc. Its great insight on how they will perform. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Monday, July 02, 2007 8:27 AM To: 'Kim Tournear'; 'Histonet' Subject: RE: [Histonet] Need Some Help All, When I interviewed for my first job out of histo school, I was sat at a microtome and had to cut sections. Seems like a good test to me!!! I've done it to others, especially if they are new in the field or registry eligible. Since ASCP/BOR has seen fit to remove the practical this is the next best way. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Tournear Sent: Sunday, July 01, 2007 11:59 AM To: Histonet Subject: RE: [Histonet] Need Some Help Eric, I have to agree with Patsy...although, just because a person has passd the exam and becomes certified (lots of book smart people out there), doesn't mean they are good (hands on application) at what they do....I guess it would have to be up to your client as to whether or not they prefer that the tech is certified or not....they might only care that the tech can do the job and do it well....good luck Kim Tournear, HT (ASCP), QIHC ( ASCP) Specialists in Dermatology Histology/Mohs Supervisor Tucson, AZ --------------------------------- Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charles.Embrey <@t> carle.com Tue Jul 3 08:32:58 2007 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Tue Jul 3 08:33:03 2007 Subject: [Histonet] Need Some Help In-Reply-To: <001e01c7bd6d$1429ff10$d00f7ca5@lurie.northwestern.edu> Message-ID: <44780C571F28624DBB446DE55C4D733A1FE503@EXCHANGEBE1.carle.com> I think this is a real concern and should not be blown off so readily. Histotechs, on occasion, cut themselves through inattention, over confidence or other factors such as say, nervousness during testing for a new job. If an employee cuts himself during work then you have to refer them to Occupational Medicine follow up with infection control and possibly workman's comp and your liability is covered because they work for you. But if you set a non-employee at a microtome (a hazardous position) and they cut their finger they might just end up owning a large part of your lab. Not really a question of wisdom on the non-employee's part but should be a question of wisdom on the employer's part. Charles Embrey, PA(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Tuesday, July 03, 2007 7:24 AM To: 'Jennifer MacDonald'; dcojita@tampabay.rr.com Cc: 'Histonet'; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Need Some Help If you've been training you should be wise enough to NOT cut yourself. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: Jennifer MacDonald [mailto:JMacDonald@mtsac.edu] Sent: Monday, July 02, 2007 5:19 PM To: dcojita@tampabay.rr.com Cc: 'Bernice Frederick'; 'Histonet'; histonet-bounces@lists.utsouthwestern.edu; 'Kim Tournear' Subject: RE: [Histonet] Need Some Help Is there any concern about the liability of non-employee cutting themselves during this test? Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu Sent by: histonet-bounces@lists.utsouthwestern.edu 07/02/2007 03:06 PM To "'Bernice Frederick'" , "'Kim Tournear'" , "'Histonet'" cc Subject RE: [Histonet] Need Some Help I agree. I have a "practical" test for new applicants as well. I have them cut 5 or so blocks, observe their cutting technique, how they choose to label the slide, mount the section, etc. Its great insight on how they will perform. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Monday, July 02, 2007 8:27 AM To: 'Kim Tournear'; 'Histonet' Subject: RE: [Histonet] Need Some Help All, When I interviewed for my first job out of histo school, I was sat at a microtome and had to cut sections. Seems like a good test to me!!! I've done it to others, especially if they are new in the field or registry eligible. Since ASCP/BOR has seen fit to remove the practical this is the next best way. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Tournear Sent: Sunday, July 01, 2007 11:59 AM To: Histonet Subject: RE: [Histonet] Need Some Help Eric, I have to agree with Patsy...although, just because a person has passd the exam and becomes certified (lots of book smart people out there), doesn't mean they are good (hands on application) at what they do....I guess it would have to be up to your client as to whether or not they prefer that the tech is certified or not....they might only care that the tech can do the job and do it well....good luck Kim Tournear, HT (ASCP), QIHC ( ASCP) Specialists in Dermatology Histology/Mohs Supervisor Tucson, AZ --------------------------------- Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Tue Jul 3 09:13:28 2007 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Tue Jul 3 09:13:39 2007 Subject: [Histonet] ALK Protein - what do you use and how does it compare? In-Reply-To: <03568ED922E71549B7C3D55569A8C089127E2E@dchq.als.local> Message-ID: Sarah, I use the Alk-1 from Dako. Catalogue # is M719501 for a 1ml vial of concentrate. You can find more info. on this at www.dakousa.com Good Luck, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Sarah O'Bryan Sent: Monday, July 02, 2007 10:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ALK Protein - what do you use and how does it compare? Hello all, I am interested in information on ALK Protein antibodies. Who's do you use, and how do you find it? Thanks in advance, Sarah O'Bryan Technical Specialist ALS NZ Ltd _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trinimaican2501 <@t> yahoo.com Tue Jul 3 09:50:25 2007 From: trinimaican2501 <@t> yahoo.com (I-sanna Gibbons) Date: Tue Jul 3 09:50:30 2007 Subject: [Histonet] Celloidin time In-Reply-To: <8C9831498D3E35E-544-19143@WEBMAIL-DC11.sysops.aol.com> Message-ID: <904992.14874.qm@web50304.mail.re2.yahoo.com> Michael, I do apologize for my tardy response! I didn't do the ordering myself but I checked the label: Collodion (Pyroxylin, Diethyl Ether) Mallinckrodt Baker, Inc. 7001 Bypass Road Paris, KY 40362 I-sanna mtitford@aol.com wrote: I-sanna somewhere asks about celloidin preparation: Celloidin is made up in 50% absolute alcohol, 50% ether. When preparing it though, it is best to let the dry celloidin stay in the absolute alcohol for 24 hours, and then add the ether. It dissolves faster that way. Celloidin is frightfully expensive these days, where did you purchase yours from? Michael Titford Pathology UDA Pathology Mobile AL USA ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Finding fabulous fares is fun. Let Yahoo! FareChase search your favorite travel sites to find flight and hotel bargains. From SDrew <@t> uwhealth.org Tue Jul 3 10:09:43 2007 From: SDrew <@t> uwhealth.org (Drew Sally A.) Date: Tue Jul 3 10:09:49 2007 Subject: [Histonet] RE: Plasma Cell Markers In-Reply-To: <661949901A768E4F9CC16D8AF8F2838CA1C64F@IS-E2K3.grhs.net> Message-ID: We use CD138(clone B-A38) from Serotec on our Ventana instruments with great results. We used to do CD56 for NK cells, but dropped it because of low request volume. We've not been asked to bring on CD38. Sally Ann Drew, MT(ASCP) IHC/ISH Laboratory University of Wisconsin Hosp. & Clinics Madison, WI 53792 (608)265-6596 -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Tuesday, July 03, 2007 9:59 AM To: Shawn Salesky; Cazares, Ruth; Gudrun Lang; Henry, Charlene; Mierow, Brett T.; Milne, Katy; Prescott, Nichole; Yeo, Richard; Anna Inman; Barnes, Sue; Becky Johnson; Boozer, Kathleen; Donna Lawson; Drew Sally A.; Dusko Trajkovic; Hall, Annette; Malam, Jacqueline; Marcia Funk; Mighnon Lashus; Mike Pence; Misty Lackey; Renee Fisher Subject: Plasma Cell Markers Hi Group, I am looking at bringing in-house 3 new plasma cell markers. CD38, CD56 and CD138. Are any of you using these and how do they work for you. Thanks for any input, Mike Pence GRMC Dept. of Path. From trinimaican2501 <@t> yahoo.com Tue Jul 3 10:10:23 2007 From: trinimaican2501 <@t> yahoo.com (I-sanna Gibbons) Date: Tue Jul 3 10:10:27 2007 Subject: [Histonet] Reprocessing? Message-ID: <41589.86737.qm@web50301.mail.re2.yahoo.com> Hi all, One of the technicians in the lab is experiencing a problem. He has processed the spleen of a rat and is encountering sectioning problems - good paraffin ribbons but without the tissue, just the outline of where the tissue should be. Is it possible for him to reverse the processing procedure, re-process? Will the tissue be able to withstand it? Thanks much I-sanna --------------------------------- Get the Yahoo! toolbar and be alerted to new email wherever you're surfing. From algranth <@t> u.arizona.edu Tue Jul 3 10:17:24 2007 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Tue Jul 3 10:19:31 2007 Subject: [Histonet] Mallory's trichrome Message-ID: <6.2.3.4.0.20070703081243.01ee8d88@algranth.inbox.email.arizona.edu> I usually make up my stains but I am admitting laziness this time - does anyone know who might sell a pre-made kit to do Mallory's Trichrome? Andi ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From derek.papalegis <@t> tufts.edu Tue Jul 3 10:29:43 2007 From: derek.papalegis <@t> tufts.edu (Derek Papalegis) Date: Tue Jul 3 10:29:51 2007 Subject: [Histonet] Reprocessing? In-Reply-To: <41589.86737.qm@web50301.mail.re2.yahoo.com> References: <41589.86737.qm@web50301.mail.re2.yahoo.com> Message-ID: <468A6B67.1080406@tufts.edu> I-sanna Gibbons wrote: > Hi all, > > One of the technicians in the lab is experiencing a problem. He has processed the spleen of a rat and is encountering sectioning problems - good paraffin ribbons but without the tissue, just the outline of where the tissue should be. > > Is it possible for him to reverse the processing procedure, re-process? Will the tissue be able to withstand it? > > Thanks much > > I-sanna > > > --------------------------------- > Get the Yahoo! toolbar and be alerted to new email wherever you're surfing. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This is a common occurance with rodent tissue in general. It is because the tissue is so lean. Put a paper towel on the cold tray and soak it with water and let the tissue sit on there for 20-30 minutes then try cutting again. It should cut no problem then. If you have to take multiple sections and go deep into the block, you might have to do this a few times. If that doesn't work, put the block in the heated water bath for a minute or 2 then put back on the cold tray. That should help as well. -Derek -- Derek Papalegis HT (ASCP) Histotechnician Division of Laboratory Animal Medicine Tufts University 136 Harrison Avenue Boston, MA 02111 phone: 617 636-2971 fax: 617 636-8354 From mpence <@t> grhs.net Tue Jul 3 10:39:34 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Jul 3 10:39:44 2007 Subject: [Histonet] Mallory's trichrome In-Reply-To: <6.2.3.4.0.20070703081243.01ee8d88@algranth.inbox.email.arizona.edu> Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C650@IS-E2K3.grhs.net> Try Poly Scientific. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea Grantham Sent: Tuesday, July 03, 2007 10:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mallory's trichrome I usually make up my stains but I am admitting laziness this time - does anyone know who might sell a pre-made kit to do Mallory's Trichrome? Andi ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> u.arizona.edu Tue Jul 3 10:52:26 2007 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Tue Jul 3 10:54:38 2007 Subject: [Histonet] Mallory's trichrome In-Reply-To: <661949901A768E4F9CC16D8AF8F2838CA1C650@IS-E2K3.grhs.net> References: <6.2.3.4.0.20070703081243.01ee8d88@algranth.inbox.email.arizona.edu> <661949901A768E4F9CC16D8AF8F2838CA1C650@IS-E2K3.grhs.net> Message-ID: <6.2.3.4.0.20070703085025.01ee9a58@algranth.inbox.email.arizona.edu> It is not in my Poly Scientific catalog - that's the first place I looked. Andi At 08:39 AM 7/3/2007, Mike Pence wrote: >Try Poly Scientific. > >Mike > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea >Grantham >Sent: Tuesday, July 03, 2007 10:17 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Mallory's trichrome > > >I usually make up my stains but I am admitting laziness this time - >does anyone know who might sell a pre-made kit to do Mallory's >Trichrome? > >Andi > > > >..................................................................... >: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : >: Sr. Research Specialist University of Arizona : >: (office: AHSC 4212) P.O. Box 245044 : >: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : >: (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : >:...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From cbass <@t> bidmc.harvard.edu Tue Jul 3 10:59:44 2007 From: cbass <@t> bidmc.harvard.edu (cbass@bidmc.harvard.edu) Date: Tue Jul 3 11:00:27 2007 Subject: [Histonet] setting up old fluorescent microscope Message-ID: <4B5700BE37215344A81185F131A848C261EB82@EVS6.its.caregroup.org> Hey Guys, I've inherited an old fluorescent microscope that I'd like to get working. I believe it is an Zeiss axioplan 2. I don't know much about it other than that. I figured I'd just plug everything in to see if it powers up first. Do you have any recommendations for powering a microscope that has been off for several years? Do I have to worry about blowing anything up!?! Thanks, Caroline From akemiat3377 <@t> yahoo.com Tue Jul 3 11:02:17 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Tue Jul 3 11:02:23 2007 Subject: [Histonet] RE: workman's comp and your liability coverage In-Reply-To: <44780C571F28624DBB446DE55C4D733A1FE503@EXCHANGEBE1.carle.com> Message-ID: <781443.92699.qm@web31309.mail.mud.yahoo.com> Charles, you put it so very eliquently! I don't think the histology managers took this fact into consideration. They may not have been aware that all states have these laws in affect. It is very difficult to assess the skills a potential candidate for a job has. Especially when the practical exam has been taken away, but there are liability issues to take into consideration. Akemi Allison-Tacha --- "Charles.Embrey" wrote: > I think this is a real concern and should not be > blown off so readily. > Histotechs, on occasion, cut themselves through > inattention, over > confidence or other factors such as say, nervousness > during testing for > a new job. If an employee cuts himself during work > then you have to > refer them to Occupational Medicine follow up with > infection control and > possibly workman's comp and your liability is > covered because they work > for you. But if you set a non-employee at a > microtome (a hazardous > position) and they cut their finger they might just > end up owning a > large part of your lab. Not really a question of > wisdom on the > non-employee's part but should be a question of > wisdom on the employer's > part. > > Charles Embrey, PA(ASCP) > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Bernice > Frederick > Sent: Tuesday, July 03, 2007 7:24 AM > To: 'Jennifer MacDonald'; dcojita@tampabay.rr.com > Cc: 'Histonet'; > histonet-bounces@lists.utsouthwestern.edu > Subject: RE: [Histonet] Need Some Help > > If you've been training you should be wise enough to > NOT cut yourself. > > Bernice Frederick HTL (ASCP) > Northwestern University > Pathology Core Facility > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > > -----Original Message----- > From: Jennifer MacDonald > [mailto:JMacDonald@mtsac.edu] > Sent: Monday, July 02, 2007 5:19 PM > To: dcojita@tampabay.rr.com > Cc: 'Bernice Frederick'; 'Histonet'; > histonet-bounces@lists.utsouthwestern.edu; 'Kim > Tournear' > Subject: RE: [Histonet] Need Some Help > > > Is there any concern about the liability of > non-employee cutting > themselves during this test? > > Jennifer MacDonald > Director, Histotechnician Training Program > Mt. San Antonio College > 1100 N. Grand Ave. > Walnut, CA 91789 > (909) 594-5611 ext. 4884 > jmacdonald@mtsac.edu > > > > > Sent by: histonet-bounces@lists.utsouthwestern.edu > 07/02/2007 03:06 PM > > To > "'Bernice Frederick'" > , "'Kim Tournear'" > , "'Histonet'" > > > cc > > > Subject > RE: [Histonet] Need Some Help > > > > > > > > I agree. I have a "practical" test for new > applicants as well. I have > them > cut 5 or so blocks, observe their cutting technique, > how they choose to > label the slide, mount the section, etc. Its great > insight on how they > will > perform. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Bernice > Frederick > Sent: Monday, July 02, 2007 8:27 AM > To: 'Kim Tournear'; 'Histonet' > Subject: RE: [Histonet] Need Some Help > > All, > When I interviewed for my first job out of histo > school, I was sat at a > microtome and had to cut sections. Seems like a good > test to me!!! I've > done it to others, especially if they are new in the > field or registry > eligible. Since ASCP/BOR has seen fit to remove the > practical this is > the next best way. > Bernice > > Bernice Frederick HTL (ASCP) > Northwestern University > Pathology Core Facility > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Kim > Tournear > Sent: Sunday, July 01, 2007 11:59 AM > To: Histonet > Subject: RE: [Histonet] Need Some Help > > Eric, > I have to agree with Patsy...although, just because > a person has passd > the exam and becomes certified (lots of book smart > people out there), > doesn't mean they are good (hands on application) at > what they do....I > guess it would have to be up to your client as to > whether or not they > prefer that the tech is certified or not....they > might only care that > the tech can do the job and do it well....good luck > > > Kim Tournear, HT (ASCP), QIHC ( ASCP) > Specialists in Dermatology > Histology/Mohs Supervisor > Tucson, AZ > > > > > --------------------------------- > Looking for a deal? Find great prices on flights and > hotels with Yahoo! > FareChase. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From JMacDonald <@t> mtsac.edu Tue Jul 3 11:29:32 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Jul 3 11:29:38 2007 Subject: [Histonet] Need Some Help In-Reply-To: <001e01c7bd6d$1429ff10$d00f7ca5@lurie.northwestern.edu> Message-ID: How many experienced histotechs can say that they have never cut themselves? Most people in an interview situation are nervous and unfamiliar with the equipment being asked to use for an impromptu demonstration of skills. Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu "Bernice Frederick" Sent by: histonet-bounces@lists.utsouthwestern.edu 07/03/2007 05:24 AM To "'Jennifer MacDonald'" , cc 'Histonet' , histonet-bounces@lists.utsouthwestern.edu Subject RE: [Histonet] Need Some Help If you've been training you should be wise enough to NOT cut yourself. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: Jennifer MacDonald [mailto:JMacDonald@mtsac.edu] Sent: Monday, July 02, 2007 5:19 PM To: dcojita@tampabay.rr.com Cc: 'Bernice Frederick'; 'Histonet'; histonet-bounces@lists.utsouthwestern.edu; 'Kim Tournear' Subject: RE: [Histonet] Need Some Help Is there any concern about the liability of non-employee cutting themselves during this test? Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu Sent by: histonet-bounces@lists.utsouthwestern.edu 07/02/2007 03:06 PM To "'Bernice Frederick'" , "'Kim Tournear'" , "'Histonet'" cc Subject RE: [Histonet] Need Some Help I agree. I have a "practical" test for new applicants as well. I have them cut 5 or so blocks, observe their cutting technique, how they choose to label the slide, mount the section, etc. Its great insight on how they will perform. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Monday, July 02, 2007 8:27 AM To: 'Kim Tournear'; 'Histonet' Subject: RE: [Histonet] Need Some Help All, When I interviewed for my first job out of histo school, I was sat at a microtome and had to cut sections. Seems like a good test to me!!! I've done it to others, especially if they are new in the field or registry eligible. Since ASCP/BOR has seen fit to remove the practical this is the next best way. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Tournear Sent: Sunday, July 01, 2007 11:59 AM To: Histonet Subject: RE: [Histonet] Need Some Help Eric, I have to agree with Patsy...although, just because a person has passd the exam and becomes certified (lots of book smart people out there), doesn't mean they are good (hands on application) at what they do....I guess it would have to be up to your client as to whether or not they prefer that the tech is certified or not....they might only care that the tech can do the job and do it well....good luck Kim Tournear, HT (ASCP), QIHC ( ASCP) Specialists in Dermatology Histology/Mohs Supervisor Tucson, AZ --------------------------------- Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Tue Jul 3 11:34:50 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Jul 3 11:34:59 2007 Subject: [Histonet] Need Some Help In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FF9D5D@sjhaexc02.sjha.org> Message-ID: One of the things that I suggest my students do is prepare a portfolio of their work. An array of their H&Es of various tissue types and special stains. This will demonstrate the way they pick up the tissue, as well as the labeling. I also tell them to be prepared to describe the tissue and the stain they are presenting. Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu "Weems, Joyce" Sent by: histonet-bounces@lists.utsouthwestern.edu 07/02/2007 05:50 PM To "Jennifer MacDonald" , cc Histonet , histonet-bounces@lists.utsouthwestern.edu Subject RE: [Histonet] Need Some Help We don't do it for that reason... Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital of Atlanta 404-851-7376 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Monday, July 02, 2007 6:19 PM To: dcojita@tampabay.rr.com Cc: 'Histonet'; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Need Some Help Is there any concern about the liability of non-employee cutting themselves during this test? Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu Sent by: histonet-bounces@lists.utsouthwestern.edu 07/02/2007 03:06 PM To "'Bernice Frederick'" , "'Kim Tournear'" , "'Histonet'" cc Subject RE: [Histonet] Need Some Help I agree. I have a "practical" test for new applicants as well. I have them cut 5 or so blocks, observe their cutting technique, how they choose to label the slide, mount the section, etc. Its great insight on how they will perform. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Monday, July 02, 2007 8:27 AM To: 'Kim Tournear'; 'Histonet' Subject: RE: [Histonet] Need Some Help All, When I interviewed for my first job out of histo school, I was sat at a microtome and had to cut sections. Seems like a good test to me!!! I've done it to others, especially if they are new in the field or registry eligible. Since ASCP/BOR has seen fit to remove the practical this is the next best way. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Tournear Sent: Sunday, July 01, 2007 11:59 AM To: Histonet Subject: RE: [Histonet] Need Some Help Eric, I have to agree with Patsy...although, just because a person has passd the exam and becomes certified (lots of book smart people out there), doesn't mean they are good (hands on application) at what they do....I guess it would have to be up to your client as to whether or not they prefer that the tech is certified or not....they might only care that the tech can do the job and do it well....good luck Kim Tournear, HT (ASCP), QIHC ( ASCP) Specialists in Dermatology Histology/Mohs Supervisor Tucson, AZ --------------------------------- Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. 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Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From derek.papalegis <@t> tufts.edu Tue Jul 3 11:38:12 2007 From: derek.papalegis <@t> tufts.edu (Derek Papalegis) Date: Tue Jul 3 11:38:16 2007 Subject: [Histonet] Need Some Help In-Reply-To: References: Message-ID: <468A7B74.7020606@tufts.edu> Jennifer MacDonald wrote: > How many experienced histotechs can say that they have never cut > themselves? Most people in an interview situation are nervous and > unfamiliar with the equipment being asked to use for an impromptu > demonstration of skills. > > > Jennifer MacDonald > Director, Histotechnician Training Program > Mt. San Antonio College > 1100 N. Grand Ave. > Walnut, CA 91789 > (909) 594-5611 ext. 4884 > jmacdonald@mtsac.edu > > > > "Bernice Frederick" > Sent by: histonet-bounces@lists.utsouthwestern.edu > 07/03/2007 05:24 AM > > To > "'Jennifer MacDonald'" , > cc > 'Histonet' , > histonet-bounces@lists.utsouthwestern.edu > Subject > RE: [Histonet] Need Some Help > > > > > > > If you've been training you should be wise enough to NOT cut yourself. > > Bernice Frederick HTL (ASCP) > Northwestern University > Pathology Core Facility > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > > -----Original Message----- > From: Jennifer MacDonald [mailto:JMacDonald@mtsac.edu] > Sent: Monday, July 02, 2007 5:19 PM > To: dcojita@tampabay.rr.com > Cc: 'Bernice Frederick'; 'Histonet'; > histonet-bounces@lists.utsouthwestern.edu; 'Kim Tournear' > Subject: RE: [Histonet] Need Some Help > > > Is there any concern about the liability of non-employee cutting > themselves during this test? > > Jennifer MacDonald > Director, Histotechnician Training Program > Mt. San Antonio College > 1100 N. Grand Ave. > Walnut, CA 91789 > (909) 594-5611 ext. 4884 > jmacdonald@mtsac.edu > > > > > Sent by: histonet-bounces@lists.utsouthwestern.edu > 07/02/2007 03:06 PM > > To > "'Bernice Frederick'" , "'Kim Tournear'" > , "'Histonet'" > > > cc > > > Subject > RE: [Histonet] Need Some Help > > > > > > > > I agree. I have a "practical" test for new applicants as well. I have > them > cut 5 or so blocks, observe their cutting technique, how they choose to > label the slide, mount the section, etc. Its great insight on how they > will > perform. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice > Frederick > Sent: Monday, July 02, 2007 8:27 AM > To: 'Kim Tournear'; 'Histonet' > Subject: RE: [Histonet] Need Some Help > > All, > When I interviewed for my first job out of histo school, I was sat at a > microtome and had to cut sections. Seems like a good test to me!!! I've > done it to others, especially if they are new in the field or registry > eligible. Since ASCP/BOR has seen fit to remove the practical this is > the next best way. > Bernice > > Bernice Frederick HTL (ASCP) > Northwestern University > Pathology Core Facility > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim > Tournear > Sent: Sunday, July 01, 2007 11:59 AM > To: Histonet > Subject: RE: [Histonet] Need Some Help > > Eric, > I have to agree with Patsy...although, just because a person has passd > the exam and becomes certified (lots of book smart people out there), > doesn't mean they are good (hands on application) at what they do....I > guess it would have to be up to your client as to whether or not they > prefer that the tech is certified or not....they might only care that > the tech can do the job and do it well....good luck > > > Kim Tournear, HT (ASCP), QIHC ( ASCP) > Specialists in Dermatology > Histology/Mohs Supervisor > Tucson, AZ > > > > > --------------------------------- > Looking for a deal? Find great prices on flights and hotels with Yahoo! > FareChase. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Are you only including cuts from a microtome? -- Derek Papalegis HT (ASCP) Histotechnician Division of Laboratory Animal Medicine Tufts University 136 Harrison Avenue Boston, MA 02111 phone: 617 636-2971 fax: 617 636-8354 From Jackie.O'Connor <@t> abbott.com Tue Jul 3 12:31:13 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Tue Jul 3 12:33:54 2007 Subject: [Histonet] Need Some Help In-Reply-To: Message-ID: In 37 years, I have cut myself twice - once 22 years ago when I was trying to turn around to gossip and cut at the same time (with a 6" steel blade with no knife edge guards that I was supposed to have in place) The second time was because I wasn't paying attention to what I was doing - again, trying to clean the edge of the blade with my fingertip instead of a gauze, turning the flywheel at the same time. Jennifer MacDonald Sent by: histonet-bounces@lists.utsouthwestern.edu 07/03/2007 11:29 AM To "Bernice Frederick" cc 'Histonet' , histonet-bounces@lists.utsouthwestern.edu, dcojita@tampabay.rr.com Subject RE: [Histonet] Need Some Help How many experienced histotechs can say that they have never cut themselves? Most people in an interview situation are nervous and unfamiliar with the equipment being asked to use for an impromptu demonstration of skills. Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu "Bernice Frederick" Sent by: histonet-bounces@lists.utsouthwestern.edu 07/03/2007 05:24 AM To "'Jennifer MacDonald'" , cc 'Histonet' , histonet-bounces@lists.utsouthwestern.edu Subject RE: [Histonet] Need Some Help If you've been training you should be wise enough to NOT cut yourself. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: Jennifer MacDonald [mailto:JMacDonald@mtsac.edu] Sent: Monday, July 02, 2007 5:19 PM To: dcojita@tampabay.rr.com Cc: 'Bernice Frederick'; 'Histonet'; histonet-bounces@lists.utsouthwestern.edu; 'Kim Tournear' Subject: RE: [Histonet] Need Some Help Is there any concern about the liability of non-employee cutting themselves during this test? Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu Sent by: histonet-bounces@lists.utsouthwestern.edu 07/02/2007 03:06 PM To "'Bernice Frederick'" , "'Kim Tournear'" , "'Histonet'" cc Subject RE: [Histonet] Need Some Help I agree. I have a "practical" test for new applicants as well. I have them cut 5 or so blocks, observe their cutting technique, how they choose to label the slide, mount the section, etc. Its great insight on how they will perform. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Monday, July 02, 2007 8:27 AM To: 'Kim Tournear'; 'Histonet' Subject: RE: [Histonet] Need Some Help All, When I interviewed for my first job out of histo school, I was sat at a microtome and had to cut sections. Seems like a good test to me!!! I've done it to others, especially if they are new in the field or registry eligible. Since ASCP/BOR has seen fit to remove the practical this is the next best way. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Tournear Sent: Sunday, July 01, 2007 11:59 AM To: Histonet Subject: RE: [Histonet] Need Some Help Eric, I have to agree with Patsy...although, just because a person has passd the exam and becomes certified (lots of book smart people out there), doesn't mean they are good (hands on application) at what they do....I guess it would have to be up to your client as to whether or not they prefer that the tech is certified or not....they might only care that the tech can do the job and do it well....good luck Kim Tournear, HT (ASCP), QIHC ( ASCP) Specialists in Dermatology Histology/Mohs Supervisor Tucson, AZ --------------------------------- Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Tue Jul 3 12:34:31 2007 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Tue Jul 3 12:34:37 2007 Subject: [Histonet] Histology Wounds In-Reply-To: Message-ID: Wow, I usually get about one good cut a year. It's like someone who paints houses for a living...if you work with paint all day long, you're going to get some paint on you. What I really love is when you have to fill out all of the paperwork for your safety officer after the cut and they ask how you can avoid cutting yourself in the future. Having run out of ideas, all I could write in the blank for my last cut was "Find a career outside of the world of histology...too many sharp edges." I'm sure she loved that answer. Glen Dawson IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jennifer MacDonald Sent: Tuesday, July 03, 2007 11:30 AM To: Bernice Frederick Cc: 'Histonet'; histonet-bounces@lists.utsouthwestern.edu; dcojita@tampabay.rr.com Subject: RE: [Histonet] Need Some Help How many experienced histotechs can say that they have never cut themselves? Most people in an interview situation are nervous and unfamiliar with the equipment being asked to use for an impromptu demonstration of skills. Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu "Bernice Frederick" Sent by: histonet-bounces@lists.utsouthwestern.edu 07/03/2007 05:24 AM To "'Jennifer MacDonald'" , cc 'Histonet' , histonet-bounces@lists.utsouthwestern.edu Subject RE: [Histonet] Need Some Help If you've been training you should be wise enough to NOT cut yourself. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: Jennifer MacDonald [mailto:JMacDonald@mtsac.edu] Sent: Monday, July 02, 2007 5:19 PM To: dcojita@tampabay.rr.com Cc: 'Bernice Frederick'; 'Histonet'; histonet-bounces@lists.utsouthwestern.edu; 'Kim Tournear' Subject: RE: [Histonet] Need Some Help Is there any concern about the liability of non-employee cutting themselves during this test? Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu Sent by: histonet-bounces@lists.utsouthwestern.edu 07/02/2007 03:06 PM To "'Bernice Frederick'" , "'Kim Tournear'" , "'Histonet'" cc Subject RE: [Histonet] Need Some Help I agree. I have a "practical" test for new applicants as well. I have them cut 5 or so blocks, observe their cutting technique, how they choose to label the slide, mount the section, etc. Its great insight on how they will perform. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Monday, July 02, 2007 8:27 AM To: 'Kim Tournear'; 'Histonet' Subject: RE: [Histonet] Need Some Help All, When I interviewed for my first job out of histo school, I was sat at a microtome and had to cut sections. Seems like a good test to me!!! I've done it to others, especially if they are new in the field or registry eligible. Since ASCP/BOR has seen fit to remove the practical this is the next best way. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Tournear Sent: Sunday, July 01, 2007 11:59 AM To: Histonet Subject: RE: [Histonet] Need Some Help Eric, I have to agree with Patsy...although, just because a person has passd the exam and becomes certified (lots of book smart people out there), doesn't mean they are good (hands on application) at what they do....I guess it would have to be up to your client as to whether or not they prefer that the tech is certified or not....they might only care that the tech can do the job and do it well....good luck Kim Tournear, HT (ASCP), QIHC ( ASCP) Specialists in Dermatology Histology/Mohs Supervisor Tucson, AZ --------------------------------- Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From settembr <@t> umdnj.edu Tue Jul 3 12:37:14 2007 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Tue Jul 3 12:38:04 2007 Subject: [Histonet] ALK Protein - what do you use and how does it compare? Message-ID: Hello Sarah, I use Dako's IVD Alk Protein Cat#: M7195 I find it works well under the following conditions: I use it at a dilution of 1:45. I pretreat the slides with Dako's Target Retrieval Solution in a steamer for 40 minutes. I incubate for 30 minutes at Room Temp and the detections system I use is Dako's Envision + mouse Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Sarah O'Bryan 07/02/07 11:00 PM >>> Hello all, I am interested in information on ALK Protein antibodies. Who's do you use, and how do you find it? Thanks in advance, Sarah O'Bryan Technical Specialist ALS NZ Ltd _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trinimaican2501 <@t> yahoo.com Tue Jul 3 15:04:22 2007 From: trinimaican2501 <@t> yahoo.com (I-sanna Gibbons) Date: Tue Jul 3 15:04:27 2007 Subject: [Histonet] vacuum impregnation shedule Message-ID: <213824.42936.qm@web50303.mail.re2.yahoo.com> Hi everyone, I'm processing the brainstem of a bat (about 7-10mm long, 3mm thick) using a double impregnation method. The paraffin wax stage comprises 4 changes of wax, 9 hours total duration. However, I would like to use 2 wax changes in a vacuum at this stage but am not sure of how to modify/ decrease the duration. Also, what pressure should the vacuum be set at? Thanks again I-sanna --------------------------------- Sick sense of humor? Visit Yahoo! TV's Comedy with an Edge to see what's on, when. From Shawn.Polson <@t> noaa.gov Tue Jul 3 16:31:31 2007 From: Shawn.Polson <@t> noaa.gov (Shawn Polson) Date: Tue Jul 3 16:31:34 2007 Subject: [Histonet] Autofluorescence Issue Message-ID: <468AC033.9080707@noaa.gov> Hello, We are running into a problem with what appears to be fairly broad spectrum autofluorescence (showing up on DAPI, FITC, and TRITC filter sets) emnating from tissues in our samples. The tissues in question are acroporid coral fixed in Z-fix. Samples were all processed by enrobing in agarose, decalcifying with EDTA, and embedding in paraffin. Our intent is to use 16S rRNA FISH to identify bacteria within these tissue sections. Does anyone know of an effective way to subtract out such autofluorescence either chemically (without damaging FISH targets) or by use of some virtual subtraction technique. Thank you, Shawn -- ========================================================================= Shawn W. Polson, M.S. Molecular Biologist, Coral Health Program Center for Coastal Environmental Health and Biomolecular Research NOAA National Ocean Service Contractor, JHT Incorporated -------------------------------- Ph.D. Candidate Marine Biomedicine and Environmental Sciences Center Molecular and Cellular Biology and Pathobiology Program Medical University of South Carolina Mailing Address: Hollings Marine Laboratory 331 Fort Johnson Rd Charleston, SC 29412 Office (843)762-8840 Lab (843)762-8959 Fax (843)762-8737 From dcojita <@t> tampabay.rr.com Tue Jul 3 17:20:58 2007 From: dcojita <@t> tampabay.rr.com (dcojita@tampabay.rr.com) Date: Tue Jul 3 17:23:08 2007 Subject: [Histonet] Reprocessing? In-Reply-To: <41589.86737.qm@web50301.mail.re2.yahoo.com> Message-ID: I found an excellent way to reprocess tissue that does not seem to harm it at all. Melt the block/tissue down, put the tissue back in the cassette and put the cassette lid back on. Through the block back into paraffin and reprocess. No need to remove the paraffin from the tissue. This process is used often in our lab and works out extremely well without harming the tissue. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of I-sanna Gibbons Sent: Tuesday, July 03, 2007 11:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reprocessing? Hi all, One of the technicians in the lab is experiencing a problem. He has processed the spleen of a rat and is encountering sectioning problems - good paraffin ribbons but without the tissue, just the outline of where the tissue should be. Is it possible for him to reverse the processing procedure, re-process? Will the tissue be able to withstand it? Thanks much I-sanna --------------------------------- Get the Yahoo! toolbar and be alerted to new email wherever you're surfing. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jason.Wiese <@t> va.gov Tue Jul 3 17:27:37 2007 From: Jason.Wiese <@t> va.gov (Wiese, Jason VHAROS) Date: Tue Jul 3 17:27:44 2007 Subject: [Histonet] Reprocessing? In-Reply-To: References: <41589.86737.qm@web50301.mail.re2.yahoo.com> Message-ID: <70EEF3D43B3C164C94037D811B2BE193E12B19@VHAV20MSGA3.v20.med.va.gov> So you are going from paraffin back into formalin? I can't see how that would possibly give you any better fixation then you already had. In fact, I can't think of any reason at all to do this process... JW -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of dcojita@tampabay.rr.com Sent: Tuesday, July 03, 2007 3:21 PM To: 'I-sanna Gibbons'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Reprocessing? I found an excellent way to reprocess tissue that does not seem to harm it at all. Melt the block/tissue down, put the tissue back in the cassette and put the cassette lid back on. Through the block back into paraffin and reprocess. No need to remove the paraffin from the tissue. This process is used often in our lab and works out extremely well without harming the tissue. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of I-sanna Gibbons Sent: Tuesday, July 03, 2007 11:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reprocessing? Hi all, One of the technicians in the lab is experiencing a problem. He has processed the spleen of a rat and is encountering sectioning problems - good paraffin ribbons but without the tissue, just the outline of where the tissue should be. Is it possible for him to reverse the processing procedure, re-process? Will the tissue be able to withstand it? Thanks much I-sanna --------------------------------- Get the Yahoo! toolbar and be alerted to new email wherever you're surfing. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmilne <@t> bccancer.bc.ca Tue Jul 3 17:37:55 2007 From: kmilne <@t> bccancer.bc.ca (Milne, Katy) Date: Tue Jul 3 17:38:01 2007 Subject: [Histonet] Shipping FFPE mouse blocks to US Message-ID: <07979E76B0869D4E8C9FE4AA9FC065780240D6F0@srvex03.phsabc.ehcnet.ca> Hi there, I need to ship some formalin-fixed paraffin embedded mouse tissue blocks to the US from Canada. I am a bit of an idiot when it comes to shipping and regulations. Essentially I would think that it wouldn't qualify under transport of dangerous goods as it is mouse tissue and formalin-fixed but I'm wondering if anyone out there has experience with shipping FFPE mouse tissue to the US that could give me a quick rundown on what is required. Thanks, Katy Milne Deeley Research Centre BC Cancer Agency From jnocito <@t> satx.rr.com Tue Jul 3 17:41:32 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Jul 3 17:41:38 2007 Subject: [Histonet] Need Some Help References: Message-ID: <001e01c7bdc3$4e7a2a90$d49eae18@yourxhtr8hvc4p> I can count the number of times I cut myself. I do remember once I just finished hand stropping my knife and was taking off the handle when I dropped it and instinctively caught it (Hey, I worked on the knife for almost an hour). It was a pretty share knife too. But my question is this: if the interviewee cuts themselves, do you hire that tech any way? Hey, I'm out of work. I have all day to think of these things. Joe ----- Original Message ----- From: "Jackie M O'Connor" To: "Jennifer MacDonald" Cc: "'Histonet'" ; ; Sent: Tuesday, July 03, 2007 12:31 PM Subject: RE: [Histonet] Need Some Help > In 37 years, I have cut myself twice - once 22 years ago when I was trying > to turn around to gossip and cut at the same time (with a 6" steel blade > with no knife edge guards that I was supposed to have in place) > The second time was because I wasn't paying attention to what I was doing > - again, trying to clean the edge of the blade with my fingertip instead > of a gauze, turning the flywheel at the same time. > > > > > Jennifer MacDonald > Sent by: histonet-bounces@lists.utsouthwestern.edu > 07/03/2007 11:29 AM > > To > "Bernice Frederick" > cc > 'Histonet' , > histonet-bounces@lists.utsouthwestern.edu, dcojita@tampabay.rr.com > Subject > RE: [Histonet] Need Some Help > > > > > > > How many experienced histotechs can say that they have never cut > themselves? Most people in an interview situation are nervous and > unfamiliar with the equipment being asked to use for an impromptu > demonstration of skills. > > > Jennifer MacDonald > Director, Histotechnician Training Program > Mt. San Antonio College > 1100 N. Grand Ave. > Walnut, CA 91789 > (909) 594-5611 ext. 4884 > jmacdonald@mtsac.edu > > > > "Bernice Frederick" > Sent by: histonet-bounces@lists.utsouthwestern.edu > 07/03/2007 05:24 AM > > To > "'Jennifer MacDonald'" , > cc > 'Histonet' , > histonet-bounces@lists.utsouthwestern.edu > Subject > RE: [Histonet] Need Some Help > > > > > > > If you've been training you should be wise enough to NOT cut yourself. > > Bernice Frederick HTL (ASCP) > Northwestern University > Pathology Core Facility > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > > -----Original Message----- > From: Jennifer MacDonald [mailto:JMacDonald@mtsac.edu] > Sent: Monday, July 02, 2007 5:19 PM > To: dcojita@tampabay.rr.com > Cc: 'Bernice Frederick'; 'Histonet'; > histonet-bounces@lists.utsouthwestern.edu; 'Kim Tournear' > Subject: RE: [Histonet] Need Some Help > > > Is there any concern about the liability of non-employee cutting > themselves during this test? > > Jennifer MacDonald > Director, Histotechnician Training Program > Mt. San Antonio College > 1100 N. Grand Ave. > Walnut, CA 91789 > (909) 594-5611 ext. 4884 > jmacdonald@mtsac.edu > > > > > Sent by: histonet-bounces@lists.utsouthwestern.edu > 07/02/2007 03:06 PM > > To > "'Bernice Frederick'" , "'Kim Tournear'" > , "'Histonet'" > > > cc > > > Subject > RE: [Histonet] Need Some Help > > > > > > > > I agree. I have a "practical" test for new applicants as well. I have > them > cut 5 or so blocks, observe their cutting technique, how they choose to > label the slide, mount the section, etc. Its great insight on how they > will > perform. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice > Frederick > Sent: Monday, July 02, 2007 8:27 AM > To: 'Kim Tournear'; 'Histonet' > Subject: RE: [Histonet] Need Some Help > > All, > When I interviewed for my first job out of histo school, I was sat at a > microtome and had to cut sections. Seems like a good test to me!!! I've > done it to others, especially if they are new in the field or registry > eligible. Since ASCP/BOR has seen fit to remove the practical this is > the next best way. > Bernice > > Bernice Frederick HTL (ASCP) > Northwestern University > Pathology Core Facility > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim > Tournear > Sent: Sunday, July 01, 2007 11:59 AM > To: Histonet > Subject: RE: [Histonet] Need Some Help > > Eric, > I have to agree with Patsy...although, just because a person has passd > the exam and becomes certified (lots of book smart people out there), > doesn't mean they are good (hands on application) at what they do....I > guess it would have to be up to your client as to whether or not they > prefer that the tech is certified or not....they might only care that > the tech can do the job and do it well....good luck > > > Kim Tournear, HT (ASCP), QIHC ( ASCP) > Specialists in Dermatology > Histology/Mohs Supervisor > Tucson, AZ > > > > > --------------------------------- > Looking for a deal? Find great prices on flights and hotels with Yahoo! > FareChase. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Tue Jul 3 17:42:52 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Jul 3 17:42:56 2007 Subject: [Histonet] Histology Wounds References: Message-ID: <002a01c7bdc3$7e1adab0$d49eae18@yourxhtr8hvc4p> I like (give me a raise because I can't pay attention). ----- Original Message ----- From: "Dawson, Glen" To: "Histonet" ; Sent: Tuesday, July 03, 2007 12:34 PM Subject: [Histonet] Histology Wounds Wow, I usually get about one good cut a year. It's like someone who paints houses for a living...if you work with paint all day long, you're going to get some paint on you. What I really love is when you have to fill out all of the paperwork for your safety officer after the cut and they ask how you can avoid cutting yourself in the future. Having run out of ideas, all I could write in the blank for my last cut was "Find a career outside of the world of histology...too many sharp edges." I'm sure she loved that answer. Glen Dawson IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jennifer MacDonald Sent: Tuesday, July 03, 2007 11:30 AM To: Bernice Frederick Cc: 'Histonet'; histonet-bounces@lists.utsouthwestern.edu; dcojita@tampabay.rr.com Subject: RE: [Histonet] Need Some Help How many experienced histotechs can say that they have never cut themselves? Most people in an interview situation are nervous and unfamiliar with the equipment being asked to use for an impromptu demonstration of skills. Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu "Bernice Frederick" Sent by: histonet-bounces@lists.utsouthwestern.edu 07/03/2007 05:24 AM To "'Jennifer MacDonald'" , cc 'Histonet' , histonet-bounces@lists.utsouthwestern.edu Subject RE: [Histonet] Need Some Help If you've been training you should be wise enough to NOT cut yourself. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: Jennifer MacDonald [mailto:JMacDonald@mtsac.edu] Sent: Monday, July 02, 2007 5:19 PM To: dcojita@tampabay.rr.com Cc: 'Bernice Frederick'; 'Histonet'; histonet-bounces@lists.utsouthwestern.edu; 'Kim Tournear' Subject: RE: [Histonet] Need Some Help Is there any concern about the liability of non-employee cutting themselves during this test? Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu Sent by: histonet-bounces@lists.utsouthwestern.edu 07/02/2007 03:06 PM To "'Bernice Frederick'" , "'Kim Tournear'" , "'Histonet'" cc Subject RE: [Histonet] Need Some Help I agree. I have a "practical" test for new applicants as well. I have them cut 5 or so blocks, observe their cutting technique, how they choose to label the slide, mount the section, etc. Its great insight on how they will perform. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Monday, July 02, 2007 8:27 AM To: 'Kim Tournear'; 'Histonet' Subject: RE: [Histonet] Need Some Help All, When I interviewed for my first job out of histo school, I was sat at a microtome and had to cut sections. Seems like a good test to me!!! I've done it to others, especially if they are new in the field or registry eligible. Since ASCP/BOR has seen fit to remove the practical this is the next best way. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Tournear Sent: Sunday, July 01, 2007 11:59 AM To: Histonet Subject: RE: [Histonet] Need Some Help Eric, I have to agree with Patsy...although, just because a person has passd the exam and becomes certified (lots of book smart people out there), doesn't mean they are good (hands on application) at what they do....I guess it would have to be up to your client as to whether or not they prefer that the tech is certified or not....they might only care that the tech can do the job and do it well....good luck Kim Tournear, HT (ASCP), QIHC ( ASCP) Specialists in Dermatology Histology/Mohs Supervisor Tucson, AZ --------------------------------- Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Tue Jul 3 17:47:38 2007 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Jul 3 17:47:51 2007 Subject: [Histonet] Reprocessing? Message-ID: Give it a go, It works very well. See: Johnson (2003) Histologic 36(1):21-22. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wiese, Jason VHAROS Sent: Wednesday, 4 July 2007 8:28 AM To: dcojita@tampabay.rr.com; I-sanna Gibbons; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Reprocessing? So you are going from paraffin back into formalin? I can't see how that would possibly give you any better fixation then you already had. In fact, I can't think of any reason at all to do this process... JW -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of dcojita@tampabay.rr.com Sent: Tuesday, July 03, 2007 3:21 PM To: 'I-sanna Gibbons'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Reprocessing? I found an excellent way to reprocess tissue that does not seem to harm it at all. Melt the block/tissue down, put the tissue back in the cassette and put the cassette lid back on. Through the block back into paraffin and reprocess. No need to remove the paraffin from the tissue. This process is used often in our lab and works out extremely well without harming the tissue. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of I-sanna Gibbons Sent: Tuesday, July 03, 2007 11:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reprocessing? Hi all, One of the technicians in the lab is experiencing a problem. He has processed the spleen of a rat and is encountering sectioning problems - good paraffin ribbons but without the tissue, just the outline of where the tissue should be. Is it possible for him to reverse the processing procedure, re-process? Will the tissue be able to withstand it? Thanks much I-sanna --------------------------------- Get the Yahoo! toolbar and be alerted to new email wherever you're surfing. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From jnocito <@t> satx.rr.com Tue Jul 3 17:48:25 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Jul 3 17:48:29 2007 Subject: [Histonet] Reprocessing? References: <41589.86737.qm@web50301.mail.re2.yahoo.com> <70EEF3D43B3C164C94037D811B2BE193E12B19@VHAV20MSGA3.v20.med.va.gov> Message-ID: <005001c7bdc4$447c6070$d49eae18@yourxhtr8hvc4p> That might be a miss print. We melt the block down, put the tissue back in the block with a lid and place the block in 10% NBF. The theory is that what was processed the firs time, won't be processed because of the paraffin. The areas that did not process will go through formalin, alcohols and Xyelene. Then the entire block gets infiltatred with paraffin. It works pretty well and doesn't seem to affect immunos. Joe ----- Original Message ----- From: "Wiese, Jason VHAROS" To: ; "I-sanna Gibbons" ; Sent: Tuesday, July 03, 2007 5:27 PM Subject: RE: [Histonet] Reprocessing? So you are going from paraffin back into formalin? I can't see how that would possibly give you any better fixation then you already had. In fact, I can't think of any reason at all to do this process... JW -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of dcojita@tampabay.rr.com Sent: Tuesday, July 03, 2007 3:21 PM To: 'I-sanna Gibbons'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Reprocessing? I found an excellent way to reprocess tissue that does not seem to harm it at all. Melt the block/tissue down, put the tissue back in the cassette and put the cassette lid back on. Through the block back into paraffin and reprocess. No need to remove the paraffin from the tissue. This process is used often in our lab and works out extremely well without harming the tissue. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of I-sanna Gibbons Sent: Tuesday, July 03, 2007 11:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reprocessing? Hi all, One of the technicians in the lab is experiencing a problem. He has processed the spleen of a rat and is encountering sectioning problems - good paraffin ribbons but without the tissue, just the outline of where the tissue should be. Is it possible for him to reverse the processing procedure, re-process? Will the tissue be able to withstand it? Thanks much I-sanna --------------------------------- Get the Yahoo! toolbar and be alerted to new email wherever you're surfing. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jason.Wiese <@t> va.gov Tue Jul 3 17:50:51 2007 From: Jason.Wiese <@t> va.gov (Wiese, Jason VHAROS) Date: Tue Jul 3 17:50:58 2007 Subject: [Histonet] Reprocessing? In-Reply-To: <005001c7bdc4$447c6070$d49eae18@yourxhtr8hvc4p> References: <41589.86737.qm@web50301.mail.re2.yahoo.com> <70EEF3D43B3C164C94037D811B2BE193E12B19@VHAV20MSGA3.v20.med.va.gov> <005001c7bdc4$447c6070$d49eae18@yourxhtr8hvc4p> Message-ID: <70EEF3D43B3C164C94037D811B2BE193E12B1B@VHAV20MSGA3.v20.med.va.gov> Cool.... I'll try it... thanks! JW -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: Tuesday, July 03, 2007 3:48 PM To: Wiese, Jason VHAROS; dcojita@tampabay.rr.com; I-sanna Gibbons; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Reprocessing? That might be a miss print. We melt the block down, put the tissue back in the block with a lid and place the block in 10% NBF. The theory is that what was processed the firs time, won't be processed because of the paraffin. The areas that did not process will go through formalin, alcohols and Xyelene. Then the entire block gets infiltatred with paraffin. It works pretty well and doesn't seem to affect immunos. Joe ----- Original Message ----- From: "Wiese, Jason VHAROS" To: ; "I-sanna Gibbons" ; Sent: Tuesday, July 03, 2007 5:27 PM Subject: RE: [Histonet] Reprocessing? So you are going from paraffin back into formalin? I can't see how that would possibly give you any better fixation then you already had. In fact, I can't think of any reason at all to do this process... JW -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of dcojita@tampabay.rr.com Sent: Tuesday, July 03, 2007 3:21 PM To: 'I-sanna Gibbons'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Reprocessing? I found an excellent way to reprocess tissue that does not seem to harm it at all. Melt the block/tissue down, put the tissue back in the cassette and put the cassette lid back on. Through the block back into paraffin and reprocess. No need to remove the paraffin from the tissue. This process is used often in our lab and works out extremely well without harming the tissue. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of I-sanna Gibbons Sent: Tuesday, July 03, 2007 11:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reprocessing? Hi all, One of the technicians in the lab is experiencing a problem. He has processed the spleen of a rat and is encountering sectioning problems - good paraffin ribbons but without the tissue, just the outline of where the tissue should be. Is it possible for him to reverse the processing procedure, re-process? Will the tissue be able to withstand it? Thanks much I-sanna --------------------------------- Get the Yahoo! toolbar and be alerted to new email wherever you're surfing. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sjt2104 <@t> columbia.edu Tue Jul 3 17:54:15 2007 From: sjt2104 <@t> columbia.edu (Sebastien Thuault) Date: Tue Jul 3 17:54:03 2007 Subject: [Histonet] HA tag detection - alternative fixative to PFA Message-ID: <005801c7bdc5$14d467e0$6a24a8c0@seblaptop> Hi, We have been trying to detect an HA-tagged protein in mouse brain slices using immunohistochemistry but we failed to detect any signal so far. There was nothing there, zero. We have tested the antibody in primary neuronal cultures and it works fine. The antibody is a monoclonal from Covance (clone 16B12 , Number MMS-101P). Does anybody have some experience with this antibody or with HA-tag detection in brain slices? Our procedure is a standard immunohistology procedure that works with many antibodies. We perfuse the brain with PFA 4% in PBS and then store the tissue overnight in PFA 4%. We cut 50 microns slices on a vibratome. The secondary antibody is coupled to Alexa 568. We excite the dye with a laser. Recently we tried to apply the exact same protocol we use for the cultures to the mouse tissue, we fixed fresh frozen section cut on a cryostat with PFA 4% for only a short amount of time (a few minutes instead of 24 hours or so). When we did that we could detect some neurons labeled but very few, in fact much fewer than expected which suggest that even with these modifications the detection was far from optimal. We think that the fixation method might be altering the antigenicity of the HA tag, what are the other fixation method that we could try for these staining? Any other suggestions? Thanks for your help! Sebastien Thuault, PhD Columbia University From talulahgosh <@t> gmail.com Tue Jul 3 22:12:47 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Jul 3 22:12:50 2007 Subject: [Histonet] Shipping FFPE mouse blocks to US In-Reply-To: References: <07979E76B0869D4E8C9FE4AA9FC065780240D6F0@srvex03.phsabc.ehcnet.ca> Message-ID: You should be able to call your EH&S people and ask (do all research facilities have a department for Environmental Health and Safety? I work for a university so I don't know if that's common procedure for everyone) A quick google search on ship formalin fixed tissue says that you can ship it regular, but I don't know if I'd rely on a google search. You could also try searching the USPS or UPS site for regulations. You could also lie about what's in the package, but that would be illegal, wouldn't it? Emily -- penguins, spoons, and you--what's life like among the flightless? http://www.getafirstlife.com/ From wulan <@t> med.kobe-u.ac.jp Wed Jul 4 00:52:58 2007 From: wulan <@t> med.kobe-u.ac.jp (Dyah Wulan Anggrahini) Date: Wed Jul 4 00:52:18 2007 Subject: [Histonet] Whole mount staining for mouse artery - NEED HELP for protocol In-Reply-To: <002301c73a57$fbc75960$6501a8c0@Patsy> Message-ID: <000001c7bdff$9342d7d0$0cc8a8c0@woelan> Dear All, I am working with mouse arteries. Recently I need to do whole mount staining of CD 31 of these arteries. Is anybody can help me with detailed protocol? I read some method from some articles but they were not clear enough for me. I would so much appreciate those who help me. I need help badly. Thanks before. Regards, Wulan Anggrahini Kobe University Japan From zumbor <@t> email.cs.nsw.gov.au Wed Jul 4 02:06:16 2007 From: zumbor <@t> email.cs.nsw.gov.au (Rosalba) Date: Wed Jul 4 02:12:41 2007 Subject: [Histonet] Premier cassettes supplier Message-ID: <01C7BE5D.A2C03550.zumbor@email.cs.nsw.gov.au> Hi All Does anyone know of a supplier for premier cassettes. Thanks Rosalba Zumbo Supervisor Histology Dept Department of Forensic Medicine 42-50 Parramatta Rd Glebe NSW 2037 Australia PH: 61 2 85847842 FAX: 61 2 95664573 zumbor@email.cs.nsw.gov.au "This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please destroy it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of the Sydney South West Area Health Service." From dcojita <@t> tampabay.rr.com Wed Jul 4 10:16:02 2007 From: dcojita <@t> tampabay.rr.com (dcojita@tampabay.rr.com) Date: Wed Jul 4 10:18:11 2007 Subject: [Histonet] Reprocessing? In-Reply-To: <70EEF3D43B3C164C94037D811B2BE193E12B1B@VHAV20MSGA3.v20.med.va.gov> Message-ID: Sorry, yes that was a misprint, I meant to say put back in formalin not paraffin. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wiese, Jason VHAROS Sent: Tuesday, July 03, 2007 6:51 PM To: Joe Nocito; dcojita@tampabay.rr.com; I-sanna Gibbons; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Reprocessing? Cool.... I'll try it... thanks! JW -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: Tuesday, July 03, 2007 3:48 PM To: Wiese, Jason VHAROS; dcojita@tampabay.rr.com; I-sanna Gibbons; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Reprocessing? That might be a miss print. We melt the block down, put the tissue back in the block with a lid and place the block in 10% NBF. The theory is that what was processed the firs time, won't be processed because of the paraffin. The areas that did not process will go through formalin, alcohols and Xyelene. Then the entire block gets infiltatred with paraffin. It works pretty well and doesn't seem to affect immunos. Joe ----- Original Message ----- From: "Wiese, Jason VHAROS" To: ; "I-sanna Gibbons" ; Sent: Tuesday, July 03, 2007 5:27 PM Subject: RE: [Histonet] Reprocessing? So you are going from paraffin back into formalin? I can't see how that would possibly give you any better fixation then you already had. In fact, I can't think of any reason at all to do this process... JW -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of dcojita@tampabay.rr.com Sent: Tuesday, July 03, 2007 3:21 PM To: 'I-sanna Gibbons'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Reprocessing? I found an excellent way to reprocess tissue that does not seem to harm it at all. Melt the block/tissue down, put the tissue back in the cassette and put the cassette lid back on. Through the block back into paraffin and reprocess. No need to remove the paraffin from the tissue. This process is used often in our lab and works out extremely well without harming the tissue. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of I-sanna Gibbons Sent: Tuesday, July 03, 2007 11:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reprocessing? Hi all, One of the technicians in the lab is experiencing a problem. He has processed the spleen of a rat and is encountering sectioning problems - good paraffin ribbons but without the tissue, just the outline of where the tissue should be. Is it possible for him to reverse the processing procedure, re-process? Will the tissue be able to withstand it? Thanks much I-sanna --------------------------------- Get the Yahoo! toolbar and be alerted to new email wherever you're surfing. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Wed Jul 4 10:30:25 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Jul 4 10:30:33 2007 Subject: [Histonet] FISH on imprints and isolated nuclei Message-ID: <004d01c7be50$3ef986f0$6412a8c0@dielangs.at> Hi, I would like to know if anyone had similar experiences. We tried to establish FISH on imprints and isolated nuclei in our lab. First we used superfrost-plus-slides like we do with the FISH-paraffin test. After hybridization we saw an awful background around the cells, really thick green or red fluorescence (depending on the filter). It looks like the nuclei were cemented in this stuff. We were clueless because with Paraffin-FISH there was no such background. Now we found out, that after heating the slides in the oven (baking slides at 56 degrees), the background disappears. So it seems, that the slide-surface is oxidised in the heat and that the hybridization-reagens can't adhere on it. On the other side the slides, that were only airdried at roomtemperature, showed the background. Can anyone confirm this? Bye Gudrun Lang From trinimaican2501 <@t> yahoo.com Wed Jul 4 11:18:32 2007 From: trinimaican2501 <@t> yahoo.com (I-sanna Gibbons) Date: Wed Jul 4 11:18:38 2007 Subject: [Histonet] Reprocessing? In-Reply-To: Message-ID: <633756.10044.qm@web50302.mail.re2.yahoo.com> Thanks a lot! Sounds simple - we'll give it a try I-sanna dcojita@tampabay.rr.com wrote: Sorry, yes that was a misprint, I meant to say put back in formalin not paraffin. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wiese, Jason VHAROS Sent: Tuesday, July 03, 2007 6:51 PM To: Joe Nocito; dcojita@tampabay.rr.com; I-sanna Gibbons; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Reprocessing? Cool.... I'll try it... thanks! JW -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: Tuesday, July 03, 2007 3:48 PM To: Wiese, Jason VHAROS; dcojita@tampabay.rr.com; I-sanna Gibbons; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Reprocessing? That might be a miss print. We melt the block down, put the tissue back in the block with a lid and place the block in 10% NBF. The theory is that what was processed the firs time, won't be processed because of the paraffin. The areas that did not process will go through formalin, alcohols and Xyelene. Then the entire block gets infiltatred with paraffin. It works pretty well and doesn't seem to affect immunos. Joe ----- Original Message ----- From: "Wiese, Jason VHAROS" To: ; "I-sanna Gibbons" ; Sent: Tuesday, July 03, 2007 5:27 PM Subject: RE: [Histonet] Reprocessing? So you are going from paraffin back into formalin? I can't see how that would possibly give you any better fixation then you already had. In fact, I can't think of any reason at all to do this process... JW -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of dcojita@tampabay.rr.com Sent: Tuesday, July 03, 2007 3:21 PM To: 'I-sanna Gibbons'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Reprocessing? I found an excellent way to reprocess tissue that does not seem to harm it at all. Melt the block/tissue down, put the tissue back in the cassette and put the cassette lid back on. Through the block back into paraffin and reprocess. No need to remove the paraffin from the tissue. This process is used often in our lab and works out extremely well without harming the tissue. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of I-sanna Gibbons Sent: Tuesday, July 03, 2007 11:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reprocessing? Hi all, One of the technicians in the lab is experiencing a problem. He has processed the spleen of a rat and is encountering sectioning problems - good paraffin ribbons but without the tissue, just the outline of where the tissue should be. Is it possible for him to reverse the processing procedure, re-process? Will the tissue be able to withstand it? Thanks much I-sanna --------------------------------- Get the Yahoo! toolbar and be alerted to new email wherever you're surfing. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better Heartthrob. Get better relationship answers from someone who knows. Yahoo! Answers - Check it out. From dfinkelstein <@t> mhri.edu.au Thu Jul 5 06:10:36 2007 From: dfinkelstein <@t> mhri.edu.au (David Finkelstein) Date: Thu Jul 5 06:02:30 2007 Subject: [Histonet] Second hand: vibratome, lenses, microscopes and spare parts Message-ID: Dear Histonet, I am searching for suppliers of second hand microscopy and histology equipment. Are there such companies? Kind Regards David Finkelstein, The Mental Health Research Institute of Victoria, 155 Oak Street, Parkville, Victoria 3052 AUSTRALIA dfinkelstein@mhri.edu.au From SecrestK <@t> wvuh.com Thu Jul 5 07:13:16 2007 From: SecrestK <@t> wvuh.com (Secrest, Kimberly) Date: Thu Jul 5 07:13:55 2007 Subject: FW: [Histonet] ALK Protein - what do you use and how does it compare? Message-ID: <7708E0A8E46BDC40B23113F97FB0FB23031DD056@nt-exchange1.wvuh.wvuhs.com> Sarah, I know Ventana offers a predilute. Although I haven't used it yet, it will eliminate the dilution process. Kim Secrest WVUH -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Dana Settembre Sent: Tuesday, July 03, 2007 1:37 PM To: Sarah O'Bryan; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] ALK Protein - what do you use and how does it compare? Hello Sarah, I use Dako's IVD Alk Protein Cat#: M7195 I find it works well under the following conditions: I use it at a dilution of 1:45. I pretreat the slides with Dako's Target Retrieval Solution in a steamer for 40 minutes. I incubate for 30 minutes at Room Temp and the detections system I use is Dako's Envision + mouse Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Sarah O'Bryan 07/02/07 11:00 PM >>> Hello all, I am interested in information on ALK Protein antibodies. Who's do you use, and how do you find it? Thanks in advance, Sarah O'Bryan Technical Specialist ALS NZ Ltd _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From azdudley <@t> hotmail.com Thu Jul 5 07:31:47 2007 From: azdudley <@t> hotmail.com (anita dudley) Date: Thu Jul 5 07:31:54 2007 Subject: [Histonet] breast fixation Message-ID: just wondering how everyone is going to deal with the cap and oncology standards that are about to come about that breast cannot fix more that 48 hrs for her2 testing? we do not work on the weekends and a breast that comes in on friday will fix too long till monday. I guess we will have to do something on sat!!!! just wondering what others are going to do? hope all had a good 4th!!!!! anita _________________________________________________________________ Don't get caught with egg on your face. Play Chicktionary!?? http://club.live.com/chicktionary.aspx?icid=chick_wlmailtextlink From Terry.Marshall <@t> rothgen.nhs.uk Thu Jul 5 07:40:56 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Thu Jul 5 07:41:48 2007 Subject: [Histonet] breast fixation Message-ID: <5C0BED61F529364E86309CADEA63FEF25E6C84@TRFT-EX01.xRothGen.nhs.uk> Don't worry, it will autolyse not fix. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita dudley Sent: 05 July 2007 13:32 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] breast fixation just wondering how everyone is going to deal with the cap and oncology standards that are about to come about that breast cannot fix more that 48 hrs for her2 testing? we do not work on the weekends and a breast that comes in on friday will fix too long till monday. I guess we will have to do something on sat!!!! just wondering what others are going to do? hope all had a good 4th!!!!! anita _________________________________________________________________ Don't get caught with egg on your face. Play Chicktionary! http://club.live.com/chicktionary.aspx?icid=chick_wlmailtextlink________ _______________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Thu Jul 5 07:43:22 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Jul 5 07:43:29 2007 Subject: [Histonet] breast fixation Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222E864@wahtntex2.waht.swest.nhs.uk> Don't worry, it will autolyse not fix. Only in the middle!! Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Peace is not the absence of conflict but the presence of creative alternatives for responding to conflict. --Dorothy Thompson This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From NSEARCY <@t> swmail.sw.org Thu Jul 5 07:49:11 2007 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Thu Jul 5 07:49:27 2007 Subject: [Histonet] breast fixation Message-ID: We are now working on Saturdays---to do the breasts. Even though his is the first place that I have worked that did not work on Saturdays----talk about a culture shock , among other things! >>> "Marshall Terry Dr,Consultant Histopathologist" 7/5/2007 7:40:56 AM >>> Don't worry, it will autolyse not fix. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita dudley Sent: 05 July 2007 13:32 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] breast fixation just wondering how everyone is going to deal with the cap and oncology standards that are about to come about that breast cannot fix more that 48 hrs for her2 testing? we do not work on the weekends and a breast that comes in on friday will fix too long till monday. I guess we will have to do something on sat!!!! just wondering what others are going to do? hope all had a good 4th!!!!! anita _________________________________________________________________ Don't get caught with egg on your face. Play Chicktionary! http://club.live.com/chicktionary.aspx?icid=chick_wlmailtextlink________ _______________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Thu Jul 5 07:52:12 2007 From: tkngflght <@t> yahoo.com (Cheryl) Date: Thu Jul 5 07:52:18 2007 Subject: [Histonet] Help--we need a short-term travel tech!! In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBF0222E864@wahtntex2.waht.swest.nhs.uk> Message-ID: <721307.75946.qm@web50909.mail.re2.yahoo.com> Hi Everyone! A lab I work with has a tech who is considering a permanent and had been delayed in accepting. This leaves them with an unexpected and critical staffing shortage. We need a temp tech for western Ohio for at least two weeks starting ASAP. It's an 11PM-7:30AM shift and you'll be embedding and cutting well-processed tissue in a big airy lab with good equipment and good processing. I've benched there a few months ago and have two of our regular temp techs already in place so you'll walk in to friendly faces. (the regulars are good folks, too--I LIKE this lab!) Please call if interested--I'm working in a lab this week so Paula will catch your call before 3 and I'll pick up after 3. Even if you're just curious--we'd like to talk with you! Cheryl Kerry, HT(ASCP) Full Staff Inc. 281.852.9457 800.756.3309 Please leave a message if you get voice mail and we'll call you back within the day. From denise.woodward <@t> uconn.edu Thu Jul 5 10:24:50 2007 From: denise.woodward <@t> uconn.edu (Woodward, Denise) Date: Thu Jul 5 10:25:59 2007 Subject: [Histonet] CISH reagent kits In-Reply-To: References: Message-ID: Hello all, We are looking to add C-ISH to our Veterinary Diagnostic Service and would like to know what C-ISH kits people are using out in the Clinical diagnostic world. Surprisingly, the vendors out there have been less than helpful with information about their products. I don't know how these people stay in business! Thanks, Denise Long Woodward, MS, HT (ASCP), HTL, QIHC Connecticut Veterinary Medical Diagnostic Laboratory University of Connecticut Dept. of Pathobiology and Veterinary Sciences 61 N. Eagleville Road Storrs, CT 06269-3089 From CIngles <@t> uwhealth.org Thu Jul 5 11:31:50 2007 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Thu Jul 5 11:32:19 2007 Subject: [Histonet] Please unsubscribe until 8/1 Message-ID: <08A0A863637F1349BBFD83A96B27A50A120062@uwhis-xchng3.uwhis.hosp.wisc.edu> From AGrobe2555 <@t> aol.com Thu Jul 5 15:55:46 2007 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Thu Jul 5 15:55:58 2007 Subject: [Histonet] Van Gieson question.... Message-ID: Good afternoon, We are doing an Elastic Van Gieson stain on FFPE tissues and have come up against a bit of a question. While the elastin seems to be staining quite nicely, the nuclei either are very faintly stained or not at all. We have shortened the differentiation time in the FeCl, with little difference. Any ideas? Thanks, Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital ************************************** See what's free at http://www.aol.com. From akemiat3377 <@t> yahoo.com Thu Jul 5 16:58:39 2007 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Thu Jul 5 16:58:48 2007 Subject: [Histonet] Van Gieson question.... In-Reply-To: Message-ID: <6799.69564.qm@web31308.mail.mud.yahoo.com> The VVG ELASTIC Stain is used for the demonstration of pathologic changes in elastic fibers. These include atrophy of the elastic tissue; thinning or loss that may result from arteriosclerotic changes; and reduplication, breaks, or splitting that may be used to demonstrate normal elastic tissue, as in the identification of veins and arteries, and to determine whether or not the blood vessels have been invaded by tumor. PRINCIPLE OF TEST: The tissue is over-stained with a soluble lake of hematoxylin-ferric chloride-iodine. Both ferric chloride and iodine serve as mordants, but they also have an oxidizing function that assists in converting hematoxylin to hematein. The mechanism of dye binding is probably by formation of hydrogen bonds, but the chemical groups reacting with the hematoxylin have not been identified. Because this method requires that the section be over-stained and then differentiated, it is a regressive method. Differentiation is accomplished by using excess mordant, or ferric chloride, to break the tissue-mordant-dye complex. The dye will be attracted to the larger amount of mordant in the differentiating solution and will be removed from the tissue. The elastic tissue has the strongest affinity for the iron-hematoxylin complex and will retain the dye longer than the other tissue elements. This allows other elements to be decolorized and the elastic fibers to remain stained. Sodium thiosulfate is used to remove excess iodine. van Gieson solution is the most commonly used counterstain, but others may be used. COMMENTS AND PRECAUTIONS: NOTE: It is not necessary to remove mercury deposits if tissues have been fixed in a mercury-containing fixative, since they will be removed by the staining solution. NOTE: To prepare Working ELASTIC Stain, the reagents must be added in order given. Prepare in a flask, swirling as each ingredient is added. This is critical! DO NOT JUST ADD IN A GRADUATED CYLINDER AND THEN POUR INTO A COPLIN JAR. This technic will give inconsistent results!! Check sections microscopically for adequate differentiation. Repeat till desired end-point. Sections may be left a little darker than actually desired, since they become slightly lighter after the iodine is removed in sodium thiosulfate. If differentiation has been carried too far, the sections may be restained, provided they have not been treated with alcohol. ***Do not leave in van Gieson Solution for extended time. ***The picric acid component decolorizes the elastic fibers. SPECIMEN REQUIREMENTS: Specimen consists of any well-fixed tissue. 10% formalin or Zenker's preferred. Cut section 3-5 microns. SOLUTIONS: 1. Hematoxylin Solution: "A" 2. Ferric Chloride Solution: "B" 3. Iodine/Iodide Solution: "C" Working ELASTIC Stain: Solution "A" 22.0 ml or 30.0 ml Solution "B" 8.0 ml or 12.0 ml Solution "C" 8.0 ml or 12.0 ml 4. Differentiation Solution: 5. 5% Sodium Thiosulfate Solution: 6. van Gieson Counterstain: STORAGE AND STABILITY: 7. Store Solutions in a dark place at room temperature (18-26?C). Hematoxylin Solution: "A", Ferric Chloride Solution: "B", Iodine/Iodide Solution: "C", Differentiation Solution are stable for 18 months. 5% Sodium Thiosulfate Solution and van Gieson Counterstain are stable for 12 months. QUALITY ASSURANCE AND CORRECTIVE ACTION GUIDELINES: Use a section of aorta embedded on edge or a cross section of a large artery. Check control slide microscopically after differentiating in ferric chloride for proper result. STANDARD STAINING METHOD: 1. Deparaffinize and hydrate to water. 2. Place slides in Working ELASTIC Stain for 15 to 30 minutes. (Working ELASTIC Stain is good for at least 24hrs). 3. Wash sections in running water until no excess stain remains on slides. Tissue sections should be intense black and slides will look dirty where they have been immersed in the staining solution. 4. Dip sections in differentiation solution 15-30 times and transfer to tap water. Check sections microscopically for adequate differentiation. Repeat till desired end-point. Sections may be left a little darker than actually desired, since they become slightly lighter after the iodine is removed in sodium thiosulfate. If differentiation has been carried too far, the sections may be restained, provided they have not been treated with alcohol. 5. Wash well in running water. 6. Place slides in Sodium thiosulfate Solution for 1 minute. 7. Wash in tap water. 8. Counterstain in van Gieson's Solution 2-5 minutes. *Longer time as solution ages. 9. Differentiate in 95% alcohol (2 changes), followed by dehydration in absolute alcohols. 10. Clear in several changes of clearing agent. 11. Mount with resinous mounting media. RESULTS: Elastic fibers Blue-black to black Nuclei Blue to black Collagen Red Other tissue elements Yellow REFERENCES: Sheehan, D.C. and Hrapchack, B.B., Eds. Theory and Practice of Histotechnology, 2nd Ed. Mosby, St. Louis, MO. Pp. 196-197 E. B. Prophet, B. Mills, J.B. Arrington, L.H. Sobin, A.F.I.P. Laboratory Methods in Histotechnology, 1994, pp134 Histotechnology A Self-Instructional Text, 2nd Ed. F. L. Carson, pp.138-139, 1996 Modifications by A. Allison, BIOCARE MEDICAL, Walnut Creek, CA, 2001 Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting & Staffing Specializing in Histology, SS, IHC, & TMA Tele: (925) 788-0900 E-Mail: akemiat3377@yahoo.com --- AGrobe2555@aol.com wrote: > Good afternoon, > We are doing an Elastic Van Gieson stain on FFPE > tissues and have come up > against a bit of a question. While the elastin > seems to be staining quite > nicely, the nuclei either are very faintly stained > or not at all. We have > shortened the differentiation time in the FeCl, with > little difference. Any ideas? > Thanks, > Albert > > Albert C. Grobe, PhD > International Heart Institute of Montana Foundation > Tissue Engineering Lab, Saint Patrick Hospital > > > > > > ************************************** See what's > free at http://www.aol.com. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mikael.niku <@t> helsinki.fi Fri Jul 6 02:50:19 2007 From: mikael.niku <@t> helsinki.fi (Mikael Niku) Date: Fri Jul 6 02:50:31 2007 Subject: [Histonet] what do people for ISH In-Reply-To: References: Message-ID: <468DF43B.60503@helsinki.fi> Hello Jennifer, Emily, and others, I think it's really best to be as careful as possible when one is starting RNA work. But if the lab has been working succesfully for years, I don't see why they should change the practices. Only if they are now looking at very rare RNAs, requiring top quality materials for maximal sensitivity, this might be relevant. DEPC treatment of solutions is in my experience unnecessary, if one uses high quality materials. So one can do without this highly toxic stuff. MilliQ water is as such RNAse free (I tested ours by incubating my RNA probes a few days at room temp... no detectable degradation). Using RNAse free glassware & tools, RNAse free chemicals, and of course gloves, the solutions will be RNAse free without DEPC. The endogenous RNAses are probably really the most serious threat to RNA. Tissues like pancreas are a nightmare, due to high enzyme content. So after killing the animal, you need to work quickly and use effective RNAse inhibitors / inactivators. We don't have separate processors, microtomes, or cryotomes for RNA work, and I'm fairly satisfied with the results. With best regards, Mikael From valeria.berno <@t> embl.it Fri Jul 6 04:27:02 2007 From: valeria.berno <@t> embl.it (Valeria Berno) Date: Fri Jul 6 04:27:13 2007 Subject: [Histonet] BUFFER In-Reply-To: <001301c7bd43$b23206f0$31ac810a@dsoclab7ros2> Message-ID: Hi there, Maybe this is due only to my little experience but I am getting a little bit confuse on the use of BUFFER!! Someone use PBS with Ca++ and Mg++, someone else w/o, others KPBS, other PEM other TBS.......and most of the time the answer of my question is "this is what we have","It always worked","there is no differences",and so on.... I believe that for instance TBS is used to modify the pH, that PEM and KPBS eliminates mild aspecific protein binding (because you have more free charged ions?) and Ca and Mg can bind to cytoskeleton protein. But I was wondering...what is the rationale in using one compared to another one. Thanks in advance for all the comments GRAZIE Valeria Berno Valeria, PhD EMBL Monterotondo Outstation via Ramarini 32 00015 Monterotondo Scalo (RM) Italy Tel: +39 06 90091 287 Fax: +39 06 90091 272 valeria.berno@embl.it No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.5.476 / Virus Database: 269.10.0/887 - Release Date: 05/07/2007 13.55 From ASelf <@t> gmhsc.com Fri Jul 6 06:14:56 2007 From: ASelf <@t> gmhsc.com (Amy Self) Date: Fri Jul 6 06:12:17 2007 Subject: [Histonet] Pathology Cost Analysis Message-ID: <39836CD6DB61654E8F95A35898C9218602E4F179@exchange.gmhpost.com> Good morning Histonetters Hope everyone had a safe and happy 4th of July... I need your help once again..... I have to do a cost analysis for histology and cytology and was wandering if anyone could help me with this? Thanks in advance.. Amy Amy Self Georgetown Hospital Systems NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From Jackie.O'Connor <@t> abbott.com Fri Jul 6 07:34:41 2007 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Jul 6 07:35:18 2007 Subject: [Histonet] Shopping for histology item In-Reply-To: <39836CD6DB61654E8F95A35898C9218602E4F179@exchange.gmhpost.com> Message-ID: I'm trying to find a vendor for foam slide file 'bumpers' or a reasonable facsimile - the kind that fit in metal slide drawers. We deal with moving thousands of slides per week, and would spend a whole day cutting up foam ourselves. They don't have to be foam - just some kind of stopper to separate groups of slides. Thanks. From talulahgosh <@t> gmail.com Fri Jul 6 07:37:51 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Jul 6 07:37:57 2007 Subject: [Histonet] what do people for ISH-depc Message-ID: The funny thing about DEPC's toxicity is that recently is has been recently demoted from highly toxic (skull and cross bones symbol) to irritant (X symbol). Same with powder paraformaldehyde. I wonder how these decisions are made. Emily -- these things happen, you know, you go for a walk in the park one day and wheelchair ninjas and nazis and pots-and-pans robots show up to kill you and dinosaurs show up to eat the remains. From gu.lang <@t> gmx.at Fri Jul 6 09:43:43 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Jul 6 09:43:53 2007 Subject: AW: [Histonet] Van Gieson question.... In-Reply-To: Message-ID: <000901c7bfdc$0d6a7760$6412a8c0@dielangs.at> The decreasing of the nuclei stain is due to the acid pH of the vanGieson. Try to prolong the incubation time in the Weigert's iron hemtaxylin, afterwards only wash in water, no differentiation and go on with the protocol to the elastica-dyesolution. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von AGrobe2555@aol.com Gesendet: Donnerstag, 05. Juli 2007 22:56 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Van Gieson question.... Good afternoon, We are doing an Elastic Van Gieson stain on FFPE tissues and have come up against a bit of a question. While the elastin seems to be staining quite nicely, the nuclei either are very faintly stained or not at all. We have shortened the differentiation time in the FeCl, with little difference. Any ideas? Thanks, Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital ************************************** See what's free at http://www.aol.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcline <@t> wchsys.org Fri Jul 6 11:52:51 2007 From: jcline <@t> wchsys.org (Joyce Cline) Date: Fri Jul 6 11:53:00 2007 Subject: [Histonet] Shopping for histology item/Jackie In-Reply-To: Message-ID: <000601c7bfee$17a06de0$1d2a14ac@wchsys.org> Try Lab Storage Systems, Inc 1-800-345-4167 Foam retainer blocks Catalog # RB-50 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Friday, July 06, 2007 8:35 AM To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] Shopping for histology item I'm trying to find a vendor for foam slide file 'bumpers' or a reasonable facsimile - the kind that fit in metal slide drawers. We deal with moving thousands of slides per week, and would spend a whole day cutting up foam ourselves. They don't have to be foam - just some kind of stopper to separate groups of slides. Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From Dorothy.L.Webb <@t> HealthPartners.Com Fri Jul 6 12:27:37 2007 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Fri Jul 6 12:27:48 2007 Subject: [Histonet] Inserts for files In-Reply-To: <01MIMRJRZ04U001SSN@Dino.HealthPartners.Com> Message-ID: <0E394B648E5284478A6CCB78E5AFDA2703640E14@hpes1.HealthPartners.int> Black "rubber" inserts for slide file drawers are obtained from Boekel Scientific, Inc. 1-800-896-8200. The part # is 901-0007. They work great!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, July 06, 2007 12:05 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 44, Issue 7 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Van Gieson question.... (AGrobe2555@aol.com) 2. Re: Van Gieson question.... (Akemi Allison-Tacha) 3. Re: what do people for ISH (Mikael Niku) 4. BUFFER (Valeria Berno) 5. Pathology Cost Analysis (Amy Self) 6. Shopping for histology item (Jackie M O'Connor) 7. Re: what do people for ISH-depc (Emily Sours) 8. AW: [Histonet] Van Gieson question.... (Gudrun Lang) 9. RE: Shopping for histology item/Jackie (Joyce Cline) ---------------------------------------------------------------------- Message: 1 Date: Thu, 5 Jul 2007 16:55:46 EDT From: AGrobe2555@aol.com Subject: [Histonet] Van Gieson question.... To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Good afternoon, We are doing an Elastic Van Gieson stain on FFPE tissues and have come up against a bit of a question. While the elastin seems to be staining quite nicely, the nuclei either are very faintly stained or not at all. We have shortened the differentiation time in the FeCl, with little difference. Any ideas? Thanks, Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital ************************************** See what's free at http://www.aol.com. ------------------------------ Message: 2 Date: Thu, 5 Jul 2007 14:58:39 -0700 (PDT) From: Akemi Allison-Tacha Subject: Re: [Histonet] Van Gieson question.... To: AGrobe2555@aol.com, histonet@lists.utsouthwestern.edu Message-ID: <6799.69564.qm@web31308.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 The VVG ELASTIC Stain is used for the demonstration of pathologic changes in elastic fibers. These include atrophy of the elastic tissue; thinning or loss that may result from arteriosclerotic changes; and reduplication, breaks, or splitting that may be used to demonstrate normal elastic tissue, as in the identification of veins and arteries, and to determine whether or not the blood vessels have been invaded by tumor. PRINCIPLE OF TEST: The tissue is over-stained with a soluble lake of hematoxylin-ferric chloride-iodine. Both ferric chloride and iodine serve as mordants, but they also have an oxidizing function that assists in converting hematoxylin to hematein. The mechanism of dye binding is probably by formation of hydrogen bonds, but the chemical groups reacting with the hematoxylin have not been identified. Because this method requires that the section be over-stained and then differentiated, it is a regressive method. Differentiation is accomplished by using excess mordant, or ferric chloride, to break the tissue-mordant-dye complex. The dye will be attracted to the larger amount of mordant in the differentiating solution and will be removed from the tissue. The elastic tissue has the strongest affinity for the iron-hematoxylin complex and will retain the dye longer than the other tissue elements. This allows other elements to be decolorized and the elastic fibers to remain stained. Sodium thiosulfate is used to remove excess iodine. van Gieson solution is the most commonly used counterstain, but others may be used. COMMENTS AND PRECAUTIONS: NOTE: It is not necessary to remove mercury deposits if tissues have been fixed in a mercury-containing fixative, since they will be removed by the staining solution. NOTE: To prepare Working ELASTIC Stain, the reagents must be added in order given. Prepare in a flask, swirling as each ingredient is added. This is critical! DO NOT JUST ADD IN A GRADUATED CYLINDER AND THEN POUR INTO A COPLIN JAR. This technic will give inconsistent results!! Check sections microscopically for adequate differentiation. Repeat till desired end-point. Sections may be left a little darker than actually desired, since they become slightly lighter after the iodine is removed in sodium thiosulfate. If differentiation has been carried too far, the sections may be restained, provided they have not been treated with alcohol. ***Do not leave in van Gieson Solution for extended time. ***The picric acid component decolorizes the elastic fibers. SPECIMEN REQUIREMENTS: Specimen consists of any well-fixed tissue. 10% formalin or Zenker's preferred. Cut section 3-5 microns. SOLUTIONS: 1. Hematoxylin Solution: "A" 2. Ferric Chloride Solution: "B" 3. Iodine/Iodide Solution: "C" Working ELASTIC Stain: Solution "A" 22.0 ml or 30.0 ml Solution "B" 8.0 ml or 12.0 ml Solution "C" 8.0 ml or 12.0 ml 4. Differentiation Solution: 5. 5% Sodium Thiosulfate Solution: 6. van Gieson Counterstain: STORAGE AND STABILITY: 7. Store Solutions in a dark place at room temperature (18-26?C). Hematoxylin Solution: "A", Ferric Chloride Solution: "B", Iodine/Iodide Solution: "C", Differentiation Solution are stable for 18 months. 5% Sodium Thiosulfate Solution and van Gieson Counterstain are stable for 12 months. QUALITY ASSURANCE AND CORRECTIVE ACTION GUIDELINES: Use a section of aorta embedded on edge or a cross section of a large artery. Check control slide microscopically after differentiating in ferric chloride for proper result. STANDARD STAINING METHOD: 1. Deparaffinize and hydrate to water. 2. Place slides in Working ELASTIC Stain for 15 to 30 minutes. (Working ELASTIC Stain is good for at least 24hrs). 3. Wash sections in running water until no excess stain remains on slides. Tissue sections should be intense black and slides will look dirty where they have been immersed in the staining solution. 4. Dip sections in differentiation solution 15-30 times and transfer to tap water. Check sections microscopically for adequate differentiation. Repeat till desired end-point. Sections may be left a little darker than actually desired, since they become slightly lighter after the iodine is removed in sodium thiosulfate. If differentiation has been carried too far, the sections may be restained, provided they have not been treated with alcohol. 5. Wash well in running water. 6. Place slides in Sodium thiosulfate Solution for 1 minute. 7. Wash in tap water. 8. Counterstain in van Gieson's Solution 2-5 minutes. *Longer time as solution ages. 9. Differentiate in 95% alcohol (2 changes), followed by dehydration in absolute alcohols. 10. Clear in several changes of clearing agent. 11. Mount with resinous mounting media. RESULTS: Elastic fibers Blue-black to black Nuclei Blue to black Collagen Red Other tissue elements Yellow REFERENCES: Sheehan, D.C. and Hrapchack, B.B., Eds. Theory and Practice of Histotechnology, 2nd Ed. Mosby, St. Louis, MO. Pp. 196-197 E. B. Prophet, B. Mills, J.B. Arrington, L.H. Sobin, A.F.I.P. Laboratory Methods in Histotechnology, 1994, pp134 Histotechnology A Self-Instructional Text, 2nd Ed. F. L. Carson, pp.138-139, 1996 Modifications by A. Allison, BIOCARE MEDICAL, Walnut Creek, CA, 2001 Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting & Staffing Specializing in Histology, SS, IHC, & TMA Tele: (925) 788-0900 E-Mail: akemiat3377@yahoo.com --- AGrobe2555@aol.com wrote: > Good afternoon, > We are doing an Elastic Van Gieson stain on FFPE > tissues and have come up > against a bit of a question. While the elastin > seems to be staining quite > nicely, the nuclei either are very faintly stained > or not at all. We have > shortened the differentiation time in the FeCl, with > little difference. Any ideas? > Thanks, > Albert > > Albert C. Grobe, PhD > International Heart Institute of Montana Foundation > Tissue Engineering Lab, Saint Patrick Hospital > > > > > > ************************************** See what's > free at http://www.aol.com. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 3 Date: Fri, 06 Jul 2007 10:50:19 +0300 From: Mikael Niku Subject: Re: [Histonet] what do people for ISH To: "Harvey, Jennifer Lynn" Cc: histonet@lists.utsouthwestern.edu Message-ID: <468DF43B.60503@helsinki.fi> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hello Jennifer, Emily, and others, I think it's really best to be as careful as possible when one is starting RNA work. But if the lab has been working succesfully for years, I don't see why they should change the practices. Only if they are now looking at very rare RNAs, requiring top quality materials for maximal sensitivity, this might be relevant. DEPC treatment of solutions is in my experience unnecessary, if one uses high quality materials. So one can do without this highly toxic stuff. MilliQ water is as such RNAse free (I tested ours by incubating my RNA probes a few days at room temp... no detectable degradation). Using RNAse free glassware & tools, RNAse free chemicals, and of course gloves, the solutions will be RNAse free without DEPC. The endogenous RNAses are probably really the most serious threat to RNA. Tissues like pancreas are a nightmare, due to high enzyme content. So after killing the animal, you need to work quickly and use effective RNAse inhibitors / inactivators. We don't have separate processors, microtomes, or cryotomes for RNA work, and I'm fairly satisfied with the results. With best regards, Mikael ------------------------------ Message: 4 Date: Fri, 6 Jul 2007 11:27:02 +0200 From: "Valeria Berno" Subject: [Histonet] BUFFER To: Message-ID: Content-Type: text/plain; charset="windows-1250" Hi there, Maybe this is due only to my little experience but I am getting a little bit confuse on the use of BUFFER!! Someone use PBS with Ca++ and Mg++, someone else w/o, others KPBS, other PEM other TBS.......and most of the time the answer of my question is "this is what we have","It always worked","there is no differences",and so on.... I believe that for instance TBS is used to modify the pH, that PEM and KPBS eliminates mild aspecific protein binding (because you have more free charged ions?) and Ca and Mg can bind to cytoskeleton protein. But I was wondering...what is the rationale in using one compared to another one. Thanks in advance for all the comments GRAZIE Valeria Berno Valeria, PhD EMBL Monterotondo Outstation via Ramarini 32 00015 Monterotondo Scalo (RM) Italy Tel: +39 06 90091 287 Fax: +39 06 90091 272 valeria.berno@embl.it No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.5.476 / Virus Database: 269.10.0/887 - Release Date: 05/07/2007 13.55 ------------------------------ Message: 5 Date: Fri, 6 Jul 2007 07:14:56 -0400 From: "Amy Self" Subject: [Histonet] Pathology Cost Analysis To: Message-ID: <39836CD6DB61654E8F95A35898C9218602E4F179@exchange.gmhpost.com> Content-Type: text/plain; charset="iso-8859-1" Good morning Histonetters Hope everyone had a safe and happy 4th of July... I need your help once again..... I have to do a cost analysis for histology and cytology and was wandering if anyone could help me with this? Thanks in advance.. Amy Amy Self Georgetown Hospital Systems NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. ------------------------------ Message: 6 Date: Fri, 6 Jul 2007 07:34:41 -0500 From: "Jackie M O'Connor" Subject: [Histonet] Shopping for histology item To: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" I'm trying to find a vendor for foam slide file 'bumpers' or a reasonable facsimile - the kind that fit in metal slide drawers. We deal with moving thousands of slides per week, and would spend a whole day cutting up foam ourselves. They don't have to be foam - just some kind of stopper to separate groups of slides. Thanks. ------------------------------ Message: 7 Date: Fri, 6 Jul 2007 08:37:51 -0400 From: "Emily Sours" Subject: Re: [Histonet] what do people for ISH-depc To: "Mikael Niku" , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1; format=flowed The funny thing about DEPC's toxicity is that recently is has been recently demoted from highly toxic (skull and cross bones symbol) to irritant (X symbol). Same with powder paraformaldehyde. I wonder how these decisions are made. Emily -- these things happen, you know, you go for a walk in the park one day and wheelchair ninjas and nazis and pots-and-pans robots show up to kill you and dinosaurs show up to eat the remains. ------------------------------ Message: 8 Date: Fri, 6 Jul 2007 16:43:43 +0200 From: "Gudrun Lang" Subject: AW: [Histonet] Van Gieson question.... To: , Message-ID: <000901c7bfdc$0d6a7760$6412a8c0@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" The decreasing of the nuclei stain is due to the acid pH of the vanGieson. Try to prolong the incubation time in the Weigert's iron hemtaxylin, afterwards only wash in water, no differentiation and go on with the protocol to the elastica-dyesolution. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von AGrobe2555@aol.com Gesendet: Donnerstag, 05. Juli 2007 22:56 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Van Gieson question.... Good afternoon, We are doing an Elastic Van Gieson stain on FFPE tissues and have come up against a bit of a question. While the elastin seems to be staining quite nicely, the nuclei either are very faintly stained or not at all. We have shortened the differentiation time in the FeCl, with little difference. Any ideas? Thanks, Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital ************************************** See what's free at http://www.aol.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Fri, 6 Jul 2007 12:52:51 -0400 From: "Joyce Cline" Subject: RE: [Histonet] Shopping for histology item/Jackie To: Message-ID: <000601c7bfee$17a06de0$1d2a14ac@wchsys.org> Content-Type: text/plain;charset="US-ASCII" Try Lab Storage Systems, Inc 1-800-345-4167 Foam retainer blocks Catalog # RB-50 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Friday, July 06, 2007 8:35 AM To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] Shopping for histology item I'm trying to find a vendor for foam slide file 'bumpers' or a reasonable facsimile - the kind that fit in metal slide drawers. We deal with moving thousands of slides per week, and would spend a whole day cutting up foam ourselves. They don't have to be foam - just some kind of stopper to separate groups of slides. Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 44, Issue 7 *************************************** ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From KHilburn <@t> compucyte.com Fri Jul 6 13:05:46 2007 From: KHilburn <@t> compucyte.com (Kate Hilburn) Date: Fri Jul 6 13:05:11 2007 Subject: [Histonet] Quantitative Imaging Cytometry Seminar in Cambridge, UK Message-ID: I want to inform you of the symposium devoted to Quantitative Imaging Cytometry on the afternoon of Wednesday, July 18 at the Cambridge Research Institute of Cancer Research UK in Cambridge, England. The registration deadline is Thursday, July 12 and cannot be extended, as the Cancer Research Institute in Cambridge is a secure facility, and arrangements to allow access to the building must be put in place in advance. No last-minute registration requests can be honored. Scheduled presentations include: * "Laser Scanning Cytometry Technology for Life Sciences and Drug Discovery" (with focus on cell-based applications, e.g., cell cycle, apoptosis, high-content pre-clinical safety and toxicology assays, rare cell analysis, immunophenotyping, etc.) - Ed Luther, Principal Scientist, CompuCyte Corporation * "LSC analysis of tissue microarrays: Application to subcellular localization of p27 and prostate cancer recurrence" - Peter Gann, Director, Department of Pathology, College of Medicine, University of Illinois at Chicago, USA * "Use of LSC technology in the department of ultrastructural pathology" - David Krull, Senior Scientist, GlaxoSmithKline, NC, USA A demonstration of the iCys(r) Research Imaging Cytometer equipped with three lasers (Violet 405nm, Blue 488nm, Red 633nm), 3PMTs, laser scatter and laser light loss detectors and confocal option based on Yokogawa spinning disk technology will be offered by appointment at the Microscopy and Imaging facility of Cancer Research UK, Cambridge Research Institute, Robinson Way, Cambridge CB2 0RE. This will be an excellent opportunity to learn from the most advanced users of Quantitative Imaging Cytometry technology in academic institutions and pharmaceutical companies. The meeting and demonstration are free of charge. Please register at http://www.compucyte.com/RegistrationForm-UK-2007.htm by Thursday, July 12. More information is available at www.compucyte.com . From mindye <@t> belairinc.com Fri Jul 6 13:24:36 2007 From: mindye <@t> belairinc.com (Mindy Ewing) Date: Fri Jul 6 13:24:47 2007 Subject: [Histonet] Unsubscribe In-Reply-To: <200707061715.l66HFMHt031683@ns-mr19.netsolmail.com> References: <200707061715.l66HFMHt031683@ns-mr19.netsolmail.com> Message-ID: <000701c7bffa$e8f15dd0$6900a8c0@1500v80> Please unscribe my email address mindye@belairinc.com Please confirm. Thanks Mindy Ewing Sales Belair Instrument Company (800) 783-9424 x146 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, July 06, 2007 1:15 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 44, Issue 7 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Van Gieson question.... (AGrobe2555@aol.com) 2. Re: Van Gieson question.... (Akemi Allison-Tacha) 3. Re: what do people for ISH (Mikael Niku) 4. BUFFER (Valeria Berno) 5. Pathology Cost Analysis (Amy Self) 6. Shopping for histology item (Jackie M O'Connor) 7. Re: what do people for ISH-depc (Emily Sours) 8. AW: [Histonet] Van Gieson question.... (Gudrun Lang) 9. RE: Shopping for histology item/Jackie (Joyce Cline) ---------------------------------------------------------------------- Message: 1 Date: Thu, 5 Jul 2007 16:55:46 EDT From: AGrobe2555@aol.com Subject: [Histonet] Van Gieson question.... To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Good afternoon, We are doing an Elastic Van Gieson stain on FFPE tissues and have come up against a bit of a question. While the elastin seems to be staining quite nicely, the nuclei either are very faintly stained or not at all. We have shortened the differentiation time in the FeCl, with little difference. Any ideas? Thanks, Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital ************************************** See what's free at http://www.aol.com. ------------------------------ Message: 2 Date: Thu, 5 Jul 2007 14:58:39 -0700 (PDT) From: Akemi Allison-Tacha Subject: Re: [Histonet] Van Gieson question.... To: AGrobe2555@aol.com, histonet@lists.utsouthwestern.edu Message-ID: <6799.69564.qm@web31308.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 The VVG ELASTIC Stain is used for the demonstration of pathologic changes in elastic fibers. These include atrophy of the elastic tissue; thinning or loss that may result from arteriosclerotic changes; and reduplication, breaks, or splitting that may be used to demonstrate normal elastic tissue, as in the identification of veins and arteries, and to determine whether or not the blood vessels have been invaded by tumor. PRINCIPLE OF TEST: The tissue is over-stained with a soluble lake of hematoxylin-ferric chloride-iodine. Both ferric chloride and iodine serve as mordants, but they also have an oxidizing function that assists in converting hematoxylin to hematein. The mechanism of dye binding is probably by formation of hydrogen bonds, but the chemical groups reacting with the hematoxylin have not been identified. Because this method requires that the section be over-stained and then differentiated, it is a regressive method. Differentiation is accomplished by using excess mordant, or ferric chloride, to break the tissue-mordant-dye complex. The dye will be attracted to the larger amount of mordant in the differentiating solution and will be removed from the tissue. The elastic tissue has the strongest affinity for the iron-hematoxylin complex and will retain the dye longer than the other tissue elements. This allows other elements to be decolorized and the elastic fibers to remain stained. Sodium thiosulfate is used to remove excess iodine. van Gieson solution is the most commonly used counterstain, but others may be used. COMMENTS AND PRECAUTIONS: NOTE: It is not necessary to remove mercury deposits if tissues have been fixed in a mercury-containing fixative, since they will be removed by the staining solution. NOTE: To prepare Working ELASTIC Stain, the reagents must be added in order given. Prepare in a flask, swirling as each ingredient is added. This is critical! DO NOT JUST ADD IN A GRADUATED CYLINDER AND THEN POUR INTO A COPLIN JAR. This technic will give inconsistent results!! Check sections microscopically for adequate differentiation. Repeat till desired end-point. Sections may be left a little darker than actually desired, since they become slightly lighter after the iodine is removed in sodium thiosulfate. If differentiation has been carried too far, the sections may be restained, provided they have not been treated with alcohol. ***Do not leave in van Gieson Solution for extended time. ***The picric acid component decolorizes the elastic fibers. SPECIMEN REQUIREMENTS: Specimen consists of any well-fixed tissue. 10% formalin or Zenker's preferred. Cut section 3-5 microns. SOLUTIONS: 1. Hematoxylin Solution: "A" 2. Ferric Chloride Solution: "B" 3. Iodine/Iodide Solution: "C" Working ELASTIC Stain: Solution "A" 22.0 ml or 30.0 ml Solution "B" 8.0 ml or 12.0 ml Solution "C" 8.0 ml or 12.0 ml 4. Differentiation Solution: 5. 5% Sodium Thiosulfate Solution: 6. van Gieson Counterstain: STORAGE AND STABILITY: 7. Store Solutions in a dark place at room temperature (18-260C). Hematoxylin Solution: "A", Ferric Chloride Solution: "B", Iodine/Iodide Solution: "C", Differentiation Solution are stable for 18 months. 5% Sodium Thiosulfate Solution and van Gieson Counterstain are stable for 12 months. QUALITY ASSURANCE AND CORRECTIVE ACTION GUIDELINES: Use a section of aorta embedded on edge or a cross section of a large artery. Check control slide microscopically after differentiating in ferric chloride for proper result. STANDARD STAINING METHOD: 1. Deparaffinize and hydrate to water. 2. Place slides in Working ELASTIC Stain for 15 to 30 minutes. (Working ELASTIC Stain is good for at least 24hrs). 3. Wash sections in running water until no excess stain remains on slides. Tissue sections should be intense black and slides will look dirty where they have been immersed in the staining solution. 4. Dip sections in differentiation solution 15-30 times and transfer to tap water. Check sections microscopically for adequate differentiation. Repeat till desired end-point. Sections may be left a little darker than actually desired, since they become slightly lighter after the iodine is removed in sodium thiosulfate. If differentiation has been carried too far, the sections may be restained, provided they have not been treated with alcohol. 5. Wash well in running water. 6. Place slides in Sodium thiosulfate Solution for 1 minute. 7. Wash in tap water. 8. Counterstain in van Gieson's Solution 2-5 minutes. *Longer time as solution ages. 9. Differentiate in 95% alcohol (2 changes), followed by dehydration in absolute alcohols. 10. Clear in several changes of clearing agent. 11. Mount with resinous mounting media. RESULTS: Elastic fibers Blue-black to black Nuclei Blue to black Collagen Red Other tissue elements Yellow REFERENCES: Sheehan, D.C. and Hrapchack, B.B., Eds. Theory and Practice of Histotechnology, 2nd Ed. Mosby, St. Louis, MO. Pp. 196-197 E. B. Prophet, B. Mills, J.B. Arrington, L.H. Sobin, A.F.I.P. Laboratory Methods in Histotechnology, 1994, pp134 Histotechnology A Self-Instructional Text, 2nd Ed. F. L. Carson, pp.138-139, 1996 Modifications by A. Allison, BIOCARE MEDICAL, Walnut Creek, CA, 2001 Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting & Staffing Specializing in Histology, SS, IHC, & TMA Tele: (925) 788-0900 E-Mail: akemiat3377@yahoo.com --- AGrobe2555@aol.com wrote: > Good afternoon, > We are doing an Elastic Van Gieson stain on FFPE > tissues and have come up > against a bit of a question. While the elastin > seems to be staining quite > nicely, the nuclei either are very faintly stained > or not at all. We have > shortened the differentiation time in the FeCl, with > little difference. Any ideas? > Thanks, > Albert > > Albert C. Grobe, PhD > International Heart Institute of Montana Foundation > Tissue Engineering Lab, Saint Patrick Hospital > > > > > > ************************************** See what's > free at http://www.aol.com. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 3 Date: Fri, 06 Jul 2007 10:50:19 +0300 From: Mikael Niku Subject: Re: [Histonet] what do people for ISH To: "Harvey, Jennifer Lynn" Cc: histonet@lists.utsouthwestern.edu Message-ID: <468DF43B.60503@helsinki.fi> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hello Jennifer, Emily, and others, I think it's really best to be as careful as possible when one is starting RNA work. But if the lab has been working succesfully for years, I don't see why they should change the practices. Only if they are now looking at very rare RNAs, requiring top quality materials for maximal sensitivity, this might be relevant. DEPC treatment of solutions is in my experience unnecessary, if one uses high quality materials. So one can do without this highly toxic stuff. MilliQ water is as such RNAse free (I tested ours by incubating my RNA probes a few days at room temp... no detectable degradation). Using RNAse free glassware & tools, RNAse free chemicals, and of course gloves, the solutions will be RNAse free without DEPC. The endogenous RNAses are probably really the most serious threat to RNA. Tissues like pancreas are a nightmare, due to high enzyme content. So after killing the animal, you need to work quickly and use effective RNAse inhibitors / inactivators. We don't have separate processors, microtomes, or cryotomes for RNA work, and I'm fairly satisfied with the results. With best regards, Mikael ------------------------------ Message: 4 Date: Fri, 6 Jul 2007 11:27:02 +0200 From: "Valeria Berno" Subject: [Histonet] BUFFER To: Message-ID: Content-Type: text/plain; charset="windows-1250" Hi there, Maybe this is due only to my little experience but I am getting a little bit confuse on the use of BUFFER!! Someone use PBS with Ca++ and Mg++, someone else w/o, others KPBS, other PEM other TBS.......and most of the time the answer of my question is "this is what we have","It always worked","there is no differences",and so on.... I believe that for instance TBS is used to modify the pH, that PEM and KPBS eliminates mild aspecific protein binding (because you have more free charged ions?) and Ca and Mg can bind to cytoskeleton protein. But I was wondering...what is the rationale in using one compared to another one. Thanks in advance for all the comments GRAZIE Valeria Berno Valeria, PhD EMBL Monterotondo Outstation via Ramarini 32 00015 Monterotondo Scalo (RM) Italy Tel: +39 06 90091 287 Fax: +39 06 90091 272 valeria.berno@embl.it No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.5.476 / Virus Database: 269.10.0/887 - Release Date: 05/07/2007 13.55 ------------------------------ Message: 5 Date: Fri, 6 Jul 2007 07:14:56 -0400 From: "Amy Self" Subject: [Histonet] Pathology Cost Analysis To: Message-ID: <39836CD6DB61654E8F95A35898C9218602E4F179@exchange.gmhpost.com> Content-Type: text/plain; charset="iso-8859-1" Good morning Histonetters Hope everyone had a safe and happy 4th of July... I need your help once again..... I have to do a cost analysis for histology and cytology and was wandering if anyone could help me with this? Thanks in advance.. Amy Amy Self Georgetown Hospital Systems NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. ------------------------------ Message: 6 Date: Fri, 6 Jul 2007 07:34:41 -0500 From: "Jackie M O'Connor" Subject: [Histonet] Shopping for histology item To: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" I'm trying to find a vendor for foam slide file 'bumpers' or a reasonable facsimile - the kind that fit in metal slide drawers. We deal with moving thousands of slides per week, and would spend a whole day cutting up foam ourselves. They don't have to be foam - just some kind of stopper to separate groups of slides. Thanks. ------------------------------ Message: 7 Date: Fri, 6 Jul 2007 08:37:51 -0400 From: "Emily Sours" Subject: Re: [Histonet] what do people for ISH-depc To: "Mikael Niku" , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1; format=flowed The funny thing about DEPC's toxicity is that recently is has been recently demoted from highly toxic (skull and cross bones symbol) to irritant (X symbol). Same with powder paraformaldehyde. I wonder how these decisions are made. Emily -- these things happen, you know, you go for a walk in the park one day and wheelchair ninjas and nazis and pots-and-pans robots show up to kill you and dinosaurs show up to eat the remains. ------------------------------ Message: 8 Date: Fri, 6 Jul 2007 16:43:43 +0200 From: "Gudrun Lang" Subject: AW: [Histonet] Van Gieson question.... To: , Message-ID: <000901c7bfdc$0d6a7760$6412a8c0@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" The decreasing of the nuclei stain is due to the acid pH of the vanGieson. Try to prolong the incubation time in the Weigert's iron hemtaxylin, afterwards only wash in water, no differentiation and go on with the protocol to the elastica-dyesolution. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr|ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von AGrobe2555@aol.com Gesendet: Donnerstag, 05. Juli 2007 22:56 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Van Gieson question.... Good afternoon, We are doing an Elastic Van Gieson stain on FFPE tissues and have come up against a bit of a question. While the elastin seems to be staining quite nicely, the nuclei either are very faintly stained or not at all. We have shortened the differentiation time in the FeCl, with little difference. Any ideas? Thanks, Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital ************************************** See what's free at http://www.aol.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Fri, 6 Jul 2007 12:52:51 -0400 From: "Joyce Cline" Subject: RE: [Histonet] Shopping for histology item/Jackie To: Message-ID: <000601c7bfee$17a06de0$1d2a14ac@wchsys.org> Content-Type: text/plain;charset="US-ASCII" Try Lab Storage Systems, Inc 1-800-345-4167 Foam retainer blocks Catalog # RB-50 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Friday, July 06, 2007 8:35 AM To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] Shopping for histology item I'm trying to find a vendor for foam slide file 'bumpers' or a reasonable facsimile - the kind that fit in metal slide drawers. We deal with moving thousands of slides per week, and would spend a whole day cutting up foam ourselves. They don't have to be foam - just some kind of stopper to separate groups of slides. Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 44, Issue 7 *************************************** From JosefaNava <@t> texashealth.org Fri Jul 6 13:31:46 2007 From: JosefaNava <@t> texashealth.org (Nava, Josefa) Date: Fri Jul 6 13:31:59 2007 Subject: [Histonet] unsubscribe Message-ID: <2C515C1049EAF5459EFD8C9B929078A401DA14FB@phdex03.txhealth.org> Please unsubscribe. Thank you The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. From Jason.Wiese <@t> va.gov Fri Jul 6 13:43:54 2007 From: Jason.Wiese <@t> va.gov (Wiese, Jason VHAROS) Date: Fri Jul 6 13:43:59 2007 Subject: [Histonet] Pancreas quick question Message-ID: <70EEF3D43B3C164C94037D811B2BE193E12B25@VHAV20MSGA3.v20.med.va.gov> Where is the highest concentration of islets of Langerhans found in the pancreas? Is it the head, the body, or the tail? Thank You! JW From galinadeyneko <@t> yahoo.com Fri Jul 6 14:52:37 2007 From: galinadeyneko <@t> yahoo.com (Galina Deyneko) Date: Fri Jul 6 14:52:43 2007 Subject: [Histonet] sucrose and formalin Message-ID: <295651.53660.qm@web33102.mail.mud.yahoo.com> Hi Kemlo and Colleagues, Very interesting information about using sucrose before formalin fixation, but do you mind to explain ,what is an application of this method and advantage over formalin fixation alone? Also in the recipe you wrote "10 ml formalin" - is it 10 %NBF or 37%.What the role of Calcium acetate ? As well I would like to share my occasionally experience. I processed formalin fixed kidneys, which were in 30% for week without intensive wash and I did not see any difference in the sectioning ( 2 microns)and H&E staining. Thank you in advance. Galina Deyneko Novartis, Cambridge MA 617-871-7613. --------------------------------- 8:00? 8:25? 8:40? Find a flick in no time with theYahoo! Search movie showtime shortcut. From scoop <@t> mail.nih.gov Fri Jul 6 15:18:57 2007 From: scoop <@t> mail.nih.gov (scoop@mail.nih.gov) Date: Fri Jul 6 15:19:05 2007 Subject: [Histonet] marker to measure if sorted or FACSed cells are contaminated? Message-ID: Dear Histonetters, I'm sorting mouse bone marrow cells (using magnetic beads). I would like to do a western on my sorted cells to determine whether the erythroid cells are contaminated with macrophages or B-cells. Does anyone know of an antibody to a mouse bone marrow macrophage marker and a mouse bone marrow B-cell marker that I can use for this purpose? I was thinking about F4/80 and CD11 but I don't know if they work in a western. Thanks in advance! Sharon From jhabecke <@t> fhcrc.org Fri Jul 6 16:05:13 2007 From: jhabecke <@t> fhcrc.org (Randolph-Habecker, Julie) Date: Fri Jul 6 16:05:21 2007 Subject: [Histonet] Canine CD56 Message-ID: Folks, Has anyone had any luck with a CD56 antibody that recognizes canine cells in either flow or IHC? Thanks in advance for your help! Julie Julie Randolph-Habecker, Ph.D. Staff Scientist - Director Experimental Histopathology Shared Resource Fred Hutchinson Cancer Research Center 1100 Fairview Ave, N. M5-A803 Seattle WA 98109-1024 206-667-6119 jhabecke@fhcrc.org From innvx <@t> sbcglobal.net Fri Jul 6 17:05:39 2007 From: innvx <@t> sbcglobal.net (INNOVEX BIOSCIENCES) Date: Fri Jul 6 17:05:45 2007 Subject: [Histonet] Animal IHC workshop Message-ID: <333263.30245.qm@web82007.mail.mud.yahoo.com> There is still space available for Animal IHC wet workshop on Friday August 17th at :1:00-4:30 PM in San Francisco, UCSF campus. This workshop is free and is CEU approved, it covers the A to Z of animal tissue IHC staining. This workshop is highly educational and it teaches the basic principals of animal tissue IHC staining. This workshop is in high demand and may not be presented in California for quite sometime. reserve your space early, To register visit Innovex at innvx.com or call 1-800-622-7808 From msviapiano <@t> yahoo.com Fri Jul 6 17:40:10 2007 From: msviapiano <@t> yahoo.com (Mariano S. Viapiano) Date: Fri Jul 6 17:40:15 2007 Subject: [Histonet] Flash-freezing samples in OCT? Message-ID: <466225.49464.qm@web54505.mail.re2.yahoo.com> Hi everyone, We just received some frozen brain tumor samples to process for IHC but are a bit confused about how they were prepared. We fix our samples in 4% PFA, change them to 30% sucrose until they sink, embed in OCT and freeze them at -80C until cryostat sectioning. On the other hand, the samples we received were fresh specimens (just out of the OR) dipped in OCT and snap-frozen in liquid nitrogen. What would be the best thing to do? Section them as they came, or thaw them, remove the OCT and do our fixation procedure before sectioning? I am a bit concerned about the OCT having acted as a ?retardant? for the snap-freezing of those samples when they were submerged in nitrogen (or am I just imagining things?) Thank you! Mariano Mariano S. Viapiano, PhD Assistant Professor Center for Molecular Neurobiology and Department of Neurological Surgery The Ohio State University 226B Rightmire Hall 1060 Carmack Rd., Columbus OH 43210 Tel (614) 292-4362 Fax (614) 292-5379 ____________________________________________________________________________________ Need a vacation? Get great deals to amazing places on Yahoo! Travel. http://travel.yahoo.com/ From jb <@t> Dal.Ca Fri Jul 6 21:54:51 2007 From: jb <@t> Dal.Ca (Joanna Borowska) Date: Fri Jul 6 21:54:59 2007 Subject: [Histonet] copper - brain Message-ID: <20070706235451.mad8q5qeynx2cgoo@my2.dal.ca> Hi everyone, Does anyone know some efficient copper stain for invertebrates tissue, especially Drosophila brain? I am interested in the histological techniques as well as fluorescent methods. Thanks in advance, Joanna Borowska Department of Psychology Life Sciences Centre, Dalhousie University From histo20 <@t> hotmail.com Sat Jul 7 08:28:43 2007 From: histo20 <@t> hotmail.com (Paula Wilder) Date: Sat Jul 7 08:28:51 2007 Subject: [Histonet] Procedure for CAM5.2 Message-ID: Hi Histonet! Does anyone have a good procedure for BD's CAM5.2 with Ventana's Ultraview kit? Thank you, Paula Wilder St. Joseph Medical Center Towson, Maryland 21204 410-337-1741 _________________________________________________________________ http://im.live.com/messenger/im/home/?source=hmtextlinkjuly07 From ladylaynah <@t> yahoo.com Sun Jul 8 17:39:41 2007 From: ladylaynah <@t> yahoo.com (Constance McManus) Date: Sun Jul 8 17:39:47 2007 Subject: [Histonet] Flash-freezing samples in OCT? Message-ID: <548652.84311.qm@web37003.mail.mud.yahoo.com> Mariano, I have been doing IHC on frozen sections and one thing I can tell you --- and I stress this point emphatically --- do not thaw the tissues, then freeze them again and try to do IHC on them. This causes the cells to lyse which in turn will destroy epitopes. Keep the tissue frozen. I would section them as they came. Connie McManus, HT Univerisity of Utah School of Medicine Dept of Dermatology ----- Original Message ---- From: Mariano S. Viapiano To: histonet@lists.utsouthwestern.edu Sent: Friday, July 6, 2007 4:40:10 PM Subject: [Histonet] Flash-freezing samples in OCT? Hi everyone, We just received some frozen brain tumor samples to process for IHC but are a bit confused about how they were prepared. We fix our samples in 4% PFA, change them to 30% sucrose until they sink, embed in OCT and freeze them at -80C until cryostat sectioning. On the other hand, the samples we received were fresh specimens (just out of the OR) dipped in OCT and snap-frozen in liquid nitrogen. What would be the best thing to do? Section them as they came, or thaw them, remove the OCT and do our fixation procedure before sectioning? I am a bit concerned about the OCT having acted as a ?retardant? for the snap-freezing of those samples when they were submerged in nitrogen (or am I just imagining things?) Thank you! Mariano Mariano S. Viapiano, PhD Assistant Professor Center for Molecular Neurobiology and Department of Neurological Surgery The Ohio State University 226B Rightmire Hall 1060 Carmack Rd., Columbus OH 43210 Tel (614) 292-4362 Fax (614) 292-5379 ____________________________________________________________________________________ Need a vacation? Get great deals to amazing places on Yahoo! Travel. http://travel.yahoo.com/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ No need to miss a message. Get email on-the-go with Yahoo! Mail for Mobile. Get started. http://mobile.yahoo.com/mail From cherie.stayner <@t> stonebow.otago.ac.nz Sun Jul 8 21:34:22 2007 From: cherie.stayner <@t> stonebow.otago.ac.nz (Cherie Stayner) Date: Sun Jul 8 21:43:35 2007 Subject: [Histonet] lectin staining Message-ID: Hi all, We are wanting to develop a lectin staining protocol using dolichos biflorus (DBA) lectin to recognize kidney collecting duct cells in culture (using immunofluorescence). We have a biotinylated lectin and a streptavidin conjugated fluorophore. We are optimising our procedure using IMCD cells but not all the cells are coming up positive. Does anyone know if the production of the sugar moieties being recognized by a lectin vary depending on the stage of the cell cycle (our cells are not synchronized), or do we just need to better optimize our protocol? Thanks, Cherie -- ------------------------------------------------------------- Cherie Stayner, PhD Research Fellow Developmental Genetics Group Department of Pathology University of Otago Dunedin 9054 NEW ZEALAND phone: +64 3 479 4129 fax: +64 3 479 7136 email: cherie.stayner@stonebow.otago.ac.nz ------------------------------------------------------------- From koellingr <@t> comcast.net Sun Jul 8 22:02:20 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Sun Jul 8 22:02:27 2007 Subject: [Histonet] marker to measure if sorted or FACSed cells are contaminated? Message-ID: <070920070302.5687.4691A53C0001F52F0000163722070206539D09020704040A0105@comcast.net> Sharon, If for CD11, you mean something like CD11b, CD11b and F4/80 would cover monocytes, granulocyte, macrophage lineage type cells and but there are other antibodies available that would show cells from that kind of linage on earlier BM cells better. Both do work in FACS, Westerns and IP as I've used them. But they won't touch your B-cell population for which you might want to try CD20 or CD45R (the B220 A-exon restricted isoform component of the CD45 complex). Also, picking out bands on a Western from (minimally) contaminating cells that have been lysed along with your target population can be problematic. Could you sort (flow) them? Magnetic beads are great and fun and doable and quick, but a cell sort I think would be better. Your e-mail appears to be NIH so there must be high-speed cell sorters in abundance around. And with the abundance of mouse BM cells, it wouldn't be a long sort. And anyone who sorts would know the antibody Ter119 which is a fantastic MOUSE erythroid marker from early to late erythroid cells. Sorting with CD11b, F4/80, B-cell markers and Ter119, gated properly, will give you some demonstrably pure erythroid populations and you would't have to search for contaminating cell lysates in Westerns. And for the IHC people Ter119, does work great by IHC, on mouse. Ray Koelling Phenopath Labs Seattle, WA -------------- Original message -------------- From: scoop@mail.nih.gov > Dear Histonetters, > > I'm sorting mouse bone marrow cells (using magnetic beads). I would > like to do a western on my sorted cells to determine whether the > erythroid cells are contaminated with macrophages or B-cells. Does > anyone know of an antibody to a mouse bone marrow macrophage marker > and a mouse bone marrow B-cell marker that I can use for this > purpose? I was thinking about F4/80 and CD11 but I don't know if > they work in a western. > > Thanks in advance! > Sharon > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Barry.R.Rittman <@t> uth.tmc.edu Sun Jul 8 22:30:22 2007 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Sun Jul 8 22:33:30 2007 Subject: [Histonet] lectin staining References: Message-ID: Cherie I would suspect that the sugar moieties do. The glycocalyx changes both during preparation for mitosis and also during all stages of differentiation for most cells. Lots of useful infomation in "Lectins". by Sharon and Lis. Will try to look for more specific information tomorrow as my copy of the book is at work. Barry ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Cherie Stayner Sent: Sun 7/8/2007 9:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] lectin staining Hi all, We are wanting to develop a lectin staining protocol using dolichos biflorus (DBA) lectin to recognize kidney collecting duct cells in culture (using immunofluorescence). We have a biotinylated lectin and a streptavidin conjugated fluorophore. We are optimising our procedure using IMCD cells but not all the cells are coming up positive. Does anyone know if the production of the sugar moieties being recognized by a lectin vary depending on the stage of the cell cycle (our cells are not synchronized), or do we just need to better optimize our protocol? Thanks, Cherie -- ------------------------------------------------------------- Cherie Stayner, PhD Research Fellow Developmental Genetics Group Department of Pathology University of Otago Dunedin 9054 NEW ZEALAND phone: +64 3 479 4129 fax: +64 3 479 7136 email: cherie.stayner@stonebow.otago.ac.nz ------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kappeler <@t> patho.unibe.ch Mon Jul 9 00:30:39 2007 From: kappeler <@t> patho.unibe.ch (Andi Kappeler) Date: Mon Jul 9 00:30:51 2007 Subject: [Histonet] Blood group antigens: IHC on FFPE tissue Message-ID: <016d01c7c1ea$490375a0$27955c82@patho.unibe.ch> Hi all We used to work with monoclonal Abs from Dako against blood group antigens A (clone 81FR2.2), B (clone 3E7), and H (clone 92FR-A2). Unfortunately, Dako has discontinued these products. Does anybody have a source for such antibodies (not necessarily same clones) that work reliably on FFPE human tissues? Many thanks! Andi Kappeler Institute of Pathology, University of Bern, Switzerland From gvdobbin <@t> ihis.org Mon Jul 9 05:57:21 2007 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Mon Jul 9 06:01:12 2007 Subject: [Histonet] Flash-freezing samples in OCT? Message-ID: Hi Mariano, I think histologically the tissues will look fine. However, snap freezing by submersion in N2 quite frequently causes the tissue blocks to crack which may present some mild sectioning issues. Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 >>> "Mariano S. Viapiano" 7/6/2007 7:40:10 PM >>> Hi everyone, We just received some frozen brain tumor samples to process for IHC but are a bit confused about how they were prepared. We fix our samples in 4% PFA, change them to 30% sucrose until they sink, embed in OCT and freeze them at -80C until cryostat sectioning. On the other hand, the samples we received were fresh specimens (just out of the OR) dipped in OCT and snap-frozen in liquid nitrogen. What would be the best thing to do? Section them as they came, or thaw them, remove the OCT and do our fixation procedure before sectioning? I am a bit concerned about the OCT having acted as a ?retardant? for the snap-freezing of those samples when they were submerged in nitrogen (or am I just imagining things?) Thank you! Mariano Mariano S. Viapiano, PhD Assistant Professor Center for Molecular Neurobiology and Department of Neurological Surgery The Ohio State University 226B Rightmire Hall 1060 Carmack Rd., Columbus OH 43210 Tel (614) 292-4362 Fax (614) 292-5379 ____________________________________________________________________________________ Need a vacation? Get great deals to amazing places on Yahoo! Travel. http://travel.yahoo.com/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ayant pour objet une personne ou un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer compl?tement de votre syst?me informatique. From LSebree <@t> uwhealth.org Mon Jul 9 07:30:12 2007 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Mon Jul 9 07:30:18 2007 Subject: [Histonet] Procedure for CAM5.2 In-Reply-To: Message-ID: Paula, You don't say for which Ventana instrument but we've always used 1 drop after Protease 1/8". Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Wilder Sent: Saturday, July 07, 2007 8:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Procedure for CAM5.2 Hi Histonet! Does anyone have a good procedure for BD's CAM5.2 with Ventana's Ultraview kit? Thank you, Paula Wilder St. Joseph Medical Center Towson, Maryland 21204 410-337-1741 _________________________________________________________________ http://im.live.com/messenger/im/home/?source=hmtextlinkjuly07 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jzack1967 <@t> yahoo.com Mon Jul 9 08:43:29 2007 From: jzack1967 <@t> yahoo.com (Jackie Zack) Date: Mon Jul 9 08:43:33 2007 Subject: [Histonet] looking for medical equip buyer Message-ID: <568650.31887.qm@web35803.mail.mud.yahoo.com> Hello all, We have a Leica TP 1050 processor, Leitz 1512 microtome and table top ventilation hood that my clinic would like to sell. Can anyone recommend a used medical equipment buyer in the Southwest. We're located in Albuquerque. Or if you are interested, feel free to contact me. Thanks, Jackie Zack Histo/Mohs Lab Manager Albuquerque Dermatology From Shirley_PHUA <@t> hsa.gov.sg Mon Jul 9 13:03:50 2007 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Mon Jul 9 13:04:15 2007 Subject: [Histonet] Shirley is away 09 July 2007 afternoon ... Message-ID: I will be out of the office from 09-07-2007 to 10-07-2007. I will return on 10 July 2007. Pathologists : I will process your requests when I return. Otherwise, if urgent, please forward your mail to henry_kyaw@hsa.gov.sg Regrets for the inconveniences caused. From audrabilbo <@t> yahoo.com Mon Jul 9 13:06:33 2007 From: audrabilbo <@t> yahoo.com (audra bilbo) Date: Mon Jul 9 13:06:38 2007 Subject: [Histonet] random review of surgical cases Message-ID: <755617.22794.qm@web32015.mail.mud.yahoo.com> How do you do a random review of surgical cases if there is only one pathologist? Audra Bilbo Pathology Services of Texarkana Texarkana, Texas ____________________________________________________________________________________ Be a better Globetrotter. Get better travel answers from someone who knows. Yahoo! Answers - Check it out. http://answers.yahoo.com/dir/?link=list&sid=396545469 From dellav <@t> musc.edu Mon Jul 9 13:31:01 2007 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Mon Jul 9 13:30:34 2007 Subject: [Histonet] Phosphofructokinase histochemical staining in skeletal muscle In-Reply-To: <755617.22794.qm@web32015.mail.mud.yahoo.com> References: <755617.22794.qm@web32015.mail.mud.yahoo.com> Message-ID: <7F6B678A32B0564196138E6B3101996A723A75@EVS1.clinlan.local> I would appreciate hearing from anyone with experience in performing phosphofructokinase staining in frozen skeletal muscle biopsies. Is phosphofructokinase especially labile or at risk for false negative staining? If so, what circumstances might contribute to this? Our muscles are transported on ice to the lab and stored in the refrigerator until they can be embedded and snap frozen. Our muscles are embedded protruding from gum tragacanth and are snap frozen in liquid nitrogen cooled 2-methyl butane. If freezing is delayed, could this contribute to negative staining? How quickly should these samples be frozen in order to prevent any diminished enzyme staining? Other issues to be concerned with when performing this technique? Sigma Chemical, our regular source, is backordered indefinitely on Fructose 1.6 diphosphate Can someone recommend another supplier for this item? thanks Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 From dprpic <@t> tripathimaging.com Mon Jul 9 14:04:41 2007 From: dprpic <@t> tripathimaging.com (Prpic, Nikki) Date: Mon Jul 9 14:05:04 2007 Subject: [Histonet] HPV typing on FFPE Message-ID: <63B33BFCAA3D434B9E5452837D523AC10147BF6D@corp-email.tripathimaging.corp> Hi all, Does anyone perform HPV typing on archived FFPE cervical samples? If so, what do you use, and how successful is it? Nikki Prpic, HT(ASCP) From ccross6032 <@t> aol.com Mon Jul 9 14:18:05 2007 From: ccross6032 <@t> aol.com (Cheryl Cross) Date: Mon Jul 9 14:18:24 2007 Subject: [Histonet] anyone doing IHC for neural migration? LAMP? Message-ID: <451A5F58-B50A-4F86-98B8-19CFBA984EDA@aol.com> HI there histonetters! Is anyone currently working with IHC markers for a neural migration study/research? I'm interested in LAMP in FFPE fetal rats to stain neurons that will become a part of the hippocampus. Thanks in advance! Cheryl Cheryl Cross, DVM, Dipl. ACVP Researcher University Corporation for Atmospheric Research College of Veterinary Medicine University of Tennessee Department of Pathology 2407 River Drive, Room A201 Knoxville, TN 37996-4542 (423) 967-2724 fax: 865-974-5616 ccross@ucar.edu From jamie.erickson <@t> abbott.com Mon Jul 9 15:36:44 2007 From: jamie.erickson <@t> abbott.com (Jamie E Erickson) Date: Mon Jul 9 15:36:51 2007 Subject: [Histonet] Freezing human skin with dry ice methanol? Message-ID: Hi All, I have a question for anyone out there that is working with frozen tissue. I have a clinical lab that I am working with to obtain skin biopsies and they wants to freeze skin biopsies with dry-ice methanol instead of the dry-ice isopentane, Will this be a problem for me sectioning and doing IHC for cytokines and membrane bound antibodies? I do not like the idea of this but I don't have any information on if it would be deleterious to freezing or do IHC this way. Anyone have any thoughts our suggestions? Thanks Jamie _______________________________ Jamie Erickson Sr. Research Associate Department: DSMP Abbott Bioresearch Center 100 Research Drive Worcester, MA 01605-4341 508-688-3134 FAX: 508-793-4895 e-mail: jamie.erickson@abbott.com From mpence <@t> grhs.net Mon Jul 9 15:47:50 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Mon Jul 9 15:47:56 2007 Subject: [Histonet] Freezing human skin with dry ice methanol? In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C660@IS-E2K3.grhs.net> WHY! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jamie E Erickson Sent: Monday, July 09, 2007 3:37 PM To: histonet histonet Subject: [Histonet] Freezing human skin with dry ice methanol? Hi All, I have a question for anyone out there that is working with frozen tissue. I have a clinical lab that I am working with to obtain skin biopsies and they wants to freeze skin biopsies with dry-ice methanol instead of the dry-ice isopentane, Will this be a problem for me sectioning and doing IHC for cytokines and membrane bound antibodies? I do not like the idea of this but I don't have any information on if it would be deleterious to freezing or do IHC this way. Anyone have any thoughts our suggestions? Thanks Jamie _______________________________ Jamie Erickson Sr. Research Associate Department: DSMP Abbott Bioresearch Center 100 Research Drive Worcester, MA 01605-4341 508-688-3134 FAX: 508-793-4895 e-mail: jamie.erickson@abbott.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Barry.R.Rittman <@t> uth.tmc.edu Mon Jul 9 15:52:59 2007 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Mon Jul 9 15:53:07 2007 Subject: [Histonet] Unsubscribe Message-ID: Thanks Barry From dcojita <@t> tampabay.rr.com Mon Jul 9 16:20:32 2007 From: dcojita <@t> tampabay.rr.com (dcojita@tampabay.rr.com) Date: Mon Jul 9 16:22:45 2007 Subject: [Histonet] random review of surgical cases In-Reply-To: <755617.22794.qm@web32015.mail.mud.yahoo.com> Message-ID: We have a few clients in that position (one pathologist at one facility) and they send their random reviews to each other. Otherwise you would have to select an outside pathologist to send to. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of audra bilbo Sent: Monday, July 09, 2007 2:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] random review of surgical cases How do you do a random review of surgical cases if there is only one pathologist? Audra Bilbo Pathology Services of Texarkana Texarkana, Texas ____________________________________________________________________________ ________ Be a better Globetrotter. Get better travel answers from someone who knows. Yahoo! Answers - Check it out. http://answers.yahoo.com/dir/?link=list&sid=396545469 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Crystalmo <@t> fmchealth.org Mon Jul 9 14:13:29 2007 From: Crystalmo <@t> fmchealth.org (Crystal Morris) Date: Mon Jul 9 20:26:16 2007 Subject: [Histonet] Surgery Requisitions Message-ID: Our surgery department has recently acquired HSM by McKesson for clinical documentation, this has caused a problem with them printing an adequate requisition from this system which does not have a single page with all the information that is required by the lab for order entry. Is there anyone else who is using this same system that could give me some insight as to how they are handling this? Thanks "Confidentiality Notice: This e-mail message, including any attachments is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review; use; disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message." From Crystalmo <@t> fmchealth.org Tue Jul 10 06:06:56 2007 From: Crystalmo <@t> fmchealth.org (Crystal Morris) Date: Tue Jul 10 06:10:17 2007 Subject: [Histonet] Methods for flash freezing Message-ID: Good morning, I am looking to get some info on flash freezing for frozen sections. Currently we use the can freeze it spray, along with the heat extractor in the cryostat, to do it faster, however I am concerned with safety and feel that this poses a huge exposure risk. We have found that using the heat extractor alone is not fast enough in cases with multiple specimens and tend to get freeze artifact, most likely due to overzealous pathologists. What is everyone else using? Do you use the canned spray? Thanks Crystal Morris M.T.(ASCP) Anatomical Supervisor Fairfield Medical Center Laboratory 401 N. Ewing St. Lancaster, Ohio 43130 (740) 687-8807 "Confidentiality Notice: This e-mail message, including any attachments is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review; use; disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message." From vazquezr <@t> ohsu.edu Tue Jul 10 08:54:52 2007 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Tue Jul 10 08:55:18 2007 Subject: [Histonet] Methods for flash freezing Message-ID: Crystal, Our department has purchased a LN2 tank and guns from a company called Polar Cryogenics. They may have other affiliates in Ohio. Saves on a lot of cost in the long run. Robyn Surgical Dermatology OHSU/Center for Health and Healing 503-494-2314 >>> "Crystal Morris" 7/10/2007 4:06 AM >>> Good morning, I am looking to get some info on flash freezing for frozen sections. Currently we use the can freeze it spray, along with the heat extractor in the cryostat, to do it faster, however I am concerned with safety and feel that this poses a huge exposure risk. We have found that using the heat extractor alone is not fast enough in cases with multiple specimens and tend to get freeze artifact, most likely due to overzealous pathologists. What is everyone else using? Do you use the canned spray? Thanks Crystal Morris M.T.(ASCP) Anatomical Supervisor Fairfield Medical Center Laboratory 401 N. Ewing St. Lancaster, Ohio 43130 (740) 687-8807 "Confidentiality Notice: This e-mail message, including any attachments is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review; use; disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message." From relia1 <@t> earthlink.net Tue Jul 10 09:14:05 2007 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Jul 10 09:14:09 2007 Subject: [Histonet] RELIA's Histology Career Bulletin 7/10/07 What are you doing for your summer vacation? Message-ID: Hi Histonetters, I hope you had a Happy 4th of July!!! What are you doing on your summer vacation? I am enjoying the beaches and the theme parks!!!!!!! If you are contemplating making a job change let me help while you kick back and enjoy a glass of iced tea. I can keep you posted on opportunities as they come open, assist you with your resume and coach you through the interview process. All of the positions I work with are fulltime 40 hour per week positions with top hospitals, labs and doctors offices. My clients offer excellent compensation including competitive salaries, great benefits, relocation assistance and in some cases sign on bonuses. My services are FREE of charge to you. All of my fees are paid by my clients, the facilities that I represent. I represent companies nationwide that are in need of histology supervisors, histotechnologists and histotechnicians. NEW GRADS are welcome to apply. Here is a list of my most exciting current openings: HISTOLOGY SUPERVISORS/MANAGERS Histology Manager ? Chicago, IL Histology Manager ? Central CA Lead Histo Tech ? San Francisco Bay Area - CA HISTOTECHNICIANS/HISTOTECHNOLOGISTS: Histo Tech - Naples, FL Dermpath Histo Tech ? Orlando, FL Histo Tech ? Maryland ? NW of DC Research Histo Tech ? Boston, MA Histo Tech South ? South TX Inside Tech Support Rep - WI Histo Tech ? Southern OK Histo Tech- Northern IL Histo Tech ? San Francisco Bay Area, CA Jr. Mohs Tech ? Los Angeles, CA Histo Tech ? WA Histo Tech ? Pittsburgh, PA Histo Tech ? KY Histo Tech ? Rochester, MN If you are interested in any of these positions please call me at 866-607-3542 or e-mail me at relia1@earthlink.net If you would like you can e-mail me your resume and a number where I can reach you at a time that is convenient for you. If you are interested in looking into new job opportunities in other areas that are not mentioned above please contact me as well. I work with facilities nationwide and I will keep your resume confidential. I will only represent you to jobs you tell me you are interested in looking into. Remember It never hurts to keep an eye open even if you are happy in your present job. Also if you know anyone else that might be interested I would really appreciate it if you would pass my information along to them as well. Thank you, Pam ? 866-607-3542 (866-60RELIA) Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net From lpaveli1 <@t> hurleymc.com Tue Jul 10 09:14:55 2007 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Tue Jul 10 09:15:44 2007 Subject: [Histonet] Methods for flash freezing Message-ID: <46935C1F020000EE00015F0B@smtp-gw.hurleymc.com> Crystal, We had very similar freezing artifacts until we purchased a Histobath from Sakuraa few years ago. Fits right on the counter. You fill it with 2-methylbutane (isopentane) and it cools it down to at least -50C. You occasionally have to replace it with fresh (I'd say every 3 months or so) as it will frost up causing the temp to rise. I wanna tell you tho', it gives the pathologists much better nuclear detail..........happy campers. Tissue samples freeze in less than 1 minute. Pricing (from Cardinal Health) was around $4440 as of 1/07. Hope this helped, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810-257-9948 fax: 810-762-7082 >>> "Crystal Morris" 07/10/07 7:06 AM >>> Good morning, I am looking to get some info on flash freezing for frozen sections. Currently we use the can freeze it spray, along with the heat extractor in the cryostat, to do it faster, however I am concerned with safety and feel that this poses a huge exposure risk. We have found that using the heat extractor alone is not fast enough in cases with multiple specimens and tend to get freeze artifact, most likely due to overzealous pathologists. What is everyone else using? Do you use the canned spray? Thanks Crystal Morris M.T.(ASCP) Anatomical Supervisor Fairfield Medical Center Laboratory 401 N. Ewing St. Lancaster, Ohio 43130 (740) 687-8807 "Confidentiality Notice: This e-mail message, including any attachments is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review; use; disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message." From asmith <@t> mail.barry.edu Tue Jul 10 09:27:15 2007 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Tue Jul 10 09:27:21 2007 Subject: [Histonet] Pancreas quick question In-Reply-To: <70EEF3D43B3C164C94037D811B2BE193E12B25@VHAV20MSGA3.v20.med.va.gov> Message-ID: <7DBCCC1FBC77C94F99F920D0CA6400B61E40B8@exchsrv01.barrynet.barry.edu> Tail. (fide 11th edition of Bloom and Fawcett, p. 721) Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wiese, Jason VHAROS Sent: Friday, July 06, 2007 2:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pancreas quick question Where is the highest concentration of islets of Langerhans found in the pancreas? Is it the head, the body, or the tail? Thank You! JW _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From mrsseagle <@t> yahoo.com Tue Jul 10 09:46:00 2007 From: mrsseagle <@t> yahoo.com (MICHELLE SEAGLE) Date: Tue Jul 10 09:46:08 2007 Subject: [Histonet] Cell Block Procedure?? Message-ID: <409904.69275.qm@web51804.mail.re2.yahoo.com> Our Histo Lab is currently in the process of starting to do cell blocks. We would be interested in knowing about any procedures that you may like to share with us about doing these. We are interested in knowing the products and companys that you use to perform these as well as the procedure. Any info that you may be able to share with uswould be greatly appreciated. Thanks, Michelle Seagle HT(ASCP) Rutherford Hospital --------------------------------- Don't pick lemons. See all the new 2007 cars at Yahoo! Autos. From froyer <@t> bitstream.net Tue Jul 10 09:50:45 2007 From: froyer <@t> bitstream.net (Ford Royer) Date: Tue Jul 10 09:51:08 2007 Subject: Need Dewar Flask (was) RE: [Histonet] Methods for flash freezing In-Reply-To: <46935C1F020000EE00015F0B@smtp-gw.hurleymc.com> References: <46935C1F020000EE00015F0B@smtp-gw.hurleymc.com> Message-ID: <003b01c7c301$b252c160$7701a80a@Ford> I?ve been following this thread and it dawned on me that I need to buy a Dewar flask to store liquid nitrogen, but I don?t need a new one from Fisher. Does anyone have a surplus cryogenic storage flask sitting around that they would like to sell? You can contact me off-List. Thanks! ~ Ford Ford M. Royer, MT(ASCP) Minnesota Medical 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL:? 612-839-1046 Phone:? 763-542-8725 Fax:? 763-546-4830 eMail:? clinicallab@minnesotamedical.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Tuesday, July 10, 2007 9:15 AM To: Crystalmo@fmchealth.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Methods for flash freezing Crystal, We had very similar freezing artifacts until we purchased a Histobath from Sakuraa few years ago. Fits right on the counter. You fill it with 2-methylbutane (isopentane) and it cools it down to at least -50C. You occasionally have to replace it with fresh (I'd say every 3 months or so) as it will frost up causing the temp to rise. I wanna tell you tho', it gives the pathologists much better nuclear detail..........happy campers. Tissue samples freeze in less than 1 minute. Pricing (from Cardinal Health) was around $4440 as of 1/07. Hope this helped, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810-257-9948 fax: 810-762-7082 From tom.read <@t> utoronto.ca Tue Jul 10 09:52:12 2007 From: tom.read <@t> utoronto.ca (Thomas Read) Date: Tue Jul 10 09:52:23 2007 Subject: [Histonet] Membrane label, fixable Message-ID: Hello, We are trying to develop a protocol to fluorescently label the cell membrane of live cultured HUVECs cells, to which we attach 4um fluorescent beads. Ideally the dye would be fixable. We are using confocal microscopy and/or deconvolution microscopy to visualize the cell membrane/bead relationship. So far we have tied RH414, WGA and even eosin and Evan's blue but not had much success. Any suggestions would be greatly appreciated. Thanks very much. Tom -- Thomas Read, PhD University of Toronto Gage Institute of Research Glaucoma Research Lab 223 College Street, Rm 308 Toronto, ON M5T 1R4 Canada E-mail: tom.read@utoronto.ca From HoustonR <@t> chi.osu.edu Tue Jul 10 11:24:29 2007 From: HoustonR <@t> chi.osu.edu (Houston, Ronald) Date: Tue Jul 10 11:25:11 2007 Subject: [Histonet] SV-40 control Message-ID: <979FF5962E234F45B06CF0DB7C1AABB210A53B4A@chi2k3ms01.columbuschildrens.net> Can someone oblige with a control block for SV-40, or polyomavirus? Thanks Ronnie Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Columbus Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 houstonr@chi.osu.edu ----------------------------------------- Confidentiality Notice: This e-mail message, from Children's Hospital, Columbus, Ohio, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes pursuant to Children's Hospital's confidentiality policies. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From plucas <@t> biopath.org Tue Jul 10 11:41:24 2007 From: plucas <@t> biopath.org (Paula Lucas) Date: Tue Jul 10 11:41:33 2007 Subject: [Histonet] glassware cleaner Message-ID: <20070710164124.E3D9E1D3B@courageux.cnchost.com> I would appreciate if anyone could recommend an excellent cleaning solution for glassware and plastics, especially for silver (gms) stained glassware. If there is a non-toxic brand available, that would be fantastic. Thank you, Paula Lucas Bio-Path Medical Group From mhanna <@t> histosearch.com Tue Jul 10 12:05:04 2007 From: mhanna <@t> histosearch.com (Marvin Hanna) Date: Tue Jul 10 12:05:11 2007 Subject: [Histonet] Histology Job Openings Message-ID: <3703448C-AC1F-43A8-A521-74FD26CBCE46@histosearch.com> Hi Histonetters, These are the latest job openings listed on Histosearch: Histologist, Molecular Pathology Laboratory Network, Inc., Maryville, Tennessee Histologist, Bronson Hospital, Kalamazoo, Michigan Histology Specialist II, Columbus Children?s Hospital, Columbus, Ohio Histotechnician (HT), Histopath, Inc., Corpus Christi, Texas Histologic Technician, Carle Clinic Association, Urbana, Illinois Vice President, Research and Development, Ikonisys, New Haven, Connecticut Supervisor- ARC Histology, St. Jude Children's Research Hospital, Memphis, Tennessee Histotechnologists, ProPath Laboratory, Dallas, Texas Best Regards, Marvin Hanna www.histosearch.com jobs.histosearch.com www.histoauctions.com From rsrichmond <@t> aol.com Tue Jul 10 12:27:13 2007 From: rsrichmond <@t> aol.com (rsrichmond@aol.com) Date: Tue Jul 10 12:27:23 2007 Subject: [Histonet] Re: random review of surgical cases In-Reply-To: <200707101300.5d4693bb39253@rly-xa03.mx.aol.com> References: <200707101300.5d4693bb39253@rly-xa03.mx.aol.com> Message-ID: <8C9912EB0159668-2B0-4830@WEBMAIL-MC05.sysops.aol.com> Audra Bilbo in Texarkana, Texas asks: >>How do you do a random review of surgical cases if there is only one pathologist?<< In my travels as a locum tenens pathologist, I have never seen this done, much less by exchanging cases between two solo pathologists. What regulatory agency requires such a review? Is there any alternative to it? It's likely to be a waste of everybody's time (except of course for the paper-pushers who require it). Bob Richmond Samurai Pathologist Knoxville, Tennessee ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From Sandra.Harrison3 <@t> va.gov Tue Jul 10 12:39:46 2007 From: Sandra.Harrison3 <@t> va.gov (Harrison, Sandra C.) Date: Tue Jul 10 12:39:51 2007 Subject: [Histonet] Formula 83 Clearing Solvent Message-ID: Dear Pathology People, If anyone has used Formula 83 Clearing Solvent, I'd like to hear your thoughts. I just received a flyer on it and it sounds of interest. Not toxic, dissolves paraffin 3 x faster than xylene substitutes, etc. I'd particularly like to know if it had any impact on your subsequent IHC testing. Thanks, Sandy From Karen.Heckford <@t> CHW.edu Tue Jul 10 13:04:25 2007 From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF) Date: Tue Jul 10 13:04:39 2007 Subject: [Histonet] HHV-8 Antibody Message-ID: I was wondering if anyone is using HHV-8 antibody with Dako Envision Dual Link on their autostainer is so which vendor are you getting your antibody from? Thanks, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathology Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu From sjt2104 <@t> columbia.edu Tue Jul 10 13:12:26 2007 From: sjt2104 <@t> columbia.edu (Sebastien Thuault) Date: Tue Jul 10 13:12:19 2007 Subject: [Histonet] alternative fixative to PFA - HA tag detection Message-ID: <001801c7c31d$e281e520$6a24a8c0@seblaptop> Hi, We have been trying to detect an HA-tagged protein in mouse brain slices using immunohistochemistry but we failed to detect any signal so far. There was nothing there, zero. We have tested the antibody in primary neuronal cultures and it works fine. The antibody is a monoclonal from Covance (clone 16B12 , Number MMS-101P). Does anybody have some experience with this antibody or with HA-tag detection in brain slices? Our procedure is a standard immunohistology procedure that works with many antibodies. We perfuse the brain with PFA 4% in PBS and then store the tissue overnight in PFA 4%. We cut 50 microns slices on a vibratome. The secondary antibody is coupled to Alexa 568. We excite the dye with a laser. Recently we tried to apply the exact same protocol we use for the cultures to the mouse tissue, we fixed fresh frozen section cut on a cryostat with PFA 4% for only a short amount of time (a few minutes instead of 24 hours or so). When we did that we could detect some neurons labeled but very few, in fact much fewer than expected which suggest that even with these modifications the detection was far from optimal. We think that the fixation method might be altering the antigenicity of the HA tag, what are the other fixation method that we could try for these staining? Any other suggestions? Thanks for your help! Sebastien Thuault, PhD Columbia University From kimtournear <@t> yahoo.com Tue Jul 10 13:14:27 2007 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Tue Jul 10 13:14:33 2007 Subject: [Histonet] Re: random review of surgical cases Message-ID: <676489.4502.qm@web50601.mail.re2.yahoo.com> Check the CLIA Manual. CLIA requires our office to do this.... probably because we process our own histology and do Mohs and we are a private lab, therefore, CAP doesn't apply to us......we have 5 dermatologists and 2 PA's in house, one of which is a board certified dermatopathologist....we pull 5 cases for each doctor and they review each others slides....we have only 1 Mohs surgeon....I pull 2 cases and send it to another local Mohs surgeon for review.....CLIA accepts this as part of our QA Plan.... this is done every 6 months and documented in a "Slide Verification" book... So the answer to your question would be to find a local pathologist to help you out on reviewing your slides....hope this helps.... Kim Tournear, HT (ASCP), QIHC ( ASCP) Specialists in Dermatology Histology/Mohs Supervisor Tucson, AZ --------------------------------- Ready for the edge of your seat? Check out tonight's top picks on Yahoo! TV. From b-frederick <@t> northwestern.edu Tue Jul 10 13:25:31 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Tue Jul 10 13:25:43 2007 Subject: [Histonet] CD31 for rabbit Message-ID: <000801c7c31f$b34f4e30$d00f7ca5@lurie.northwestern.edu> Anybody out there know of a CD31 that will cross react with rabbit? I'm hoping I can work out the mouse anti-human. Right!!! Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 From AGrobe2555 <@t> aol.com Tue Jul 10 13:50:49 2007 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Tue Jul 10 13:50:58 2007 Subject: [Histonet] RE: Membrane label, fixable Message-ID: Tom, If I recollect rightly, there are compounds that can be incorporated into the membrane of living cells and used for tracking. I have info on 2 products from SIGMA that may work for you. They are: PKH26 Red Fluorescent Cell Linker kit for general membrane labeling (PKH26-GL) PKH67 Green Fluorescent Cell Linker Kit for general membrane labeling (Stock # PKH67-GL) I have not used these products, so I can't speak to how well they work or if they are fixable, but it may get you started.... Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. ************************************** See what's free at http://www.aol.com. From HoustonR <@t> chi.osu.edu Tue Jul 10 13:55:10 2007 From: HoustonR <@t> chi.osu.edu (Houston, Ronald) Date: Tue Jul 10 13:55:50 2007 Subject: [Histonet] Formula 83 Clearing Solvent In-Reply-To: Message-ID: <979FF5962E234F45B06CF0DB7C1AABB210A53C61@chi2k3ms01.columbuschildrens.net> I tried it in a previous job in Virginia. No negative impact on processing, staining or IHC; however one of my techs was constantly nauseous with it so we never pursued past the evaluation Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Columbus Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 houstonr@chi.osu.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Harrison, Sandra C. Sent: Tuesday, July 10, 2007 1:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formula 83 Clearing Solvent Dear Pathology People, If anyone has used Formula 83 Clearing Solvent, I'd like to hear your thoughts. I just received a flyer on it and it sounds of interest. Not toxic, dissolves paraffin 3 x faster than xylene substitutes, etc. I'd particularly like to know if it had any impact on your subsequent IHC testing. Thanks, Sandy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: This e-mail message, from Children's Hospital, Columbus, Ohio, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes pursuant to Children's Hospital's confidentiality policies. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From jcline <@t> wchsys.org Tue Jul 10 16:05:21 2007 From: jcline <@t> wchsys.org (Joyce Cline) Date: Tue Jul 10 16:05:26 2007 Subject: [Histonet] Formula 83 Clearing Solvent/Sandra In-Reply-To: Message-ID: <001601c7c336$0763f4b0$1d2a14ac@wchsys.org> Hello Sandy, I am currently using Formula 83, closest to Xylene that I have found. We can file our slides in 7 to 10 days. Beats the two weeks with other substitutes and it can be recycled extremely well. No affect on IHC. We use it in the processor and the stainer. We rarely have to recoverslip. I have used substitutes for over 25 years and this so far has been the best. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Harrison, Sandra C. Sent: Tuesday, July 10, 2007 1:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formula 83 Clearing Solvent Dear Pathology People, If anyone has used Formula 83 Clearing Solvent, I'd like to hear your thoughts. I just received a flyer on it and it sounds of interest. Not toxic, dissolves paraffin 3 x faster than xylene substitutes, etc. I'd particularly like to know if it had any impact on your subsequent IHC testing. Thanks, Sandy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From AnthonyH <@t> chw.edu.au Tue Jul 10 16:50:15 2007 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Jul 10 16:50:28 2007 Subject: [Histonet] Methods for flash freezing Message-ID: We use liquid notrogen, easy and very quick. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Crystal Morris Sent: Tuesday, 10 July 2007 9:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Methods for flash freezing Good morning, I am looking to get some info on flash freezing for frozen sections. Currently we use the can freeze it spray, along with the heat extractor in the cryostat, to do it faster, however I am concerned with safety and feel that this poses a huge exposure risk. We have found that using the heat extractor alone is not fast enough in cases with multiple specimens and tend to get freeze artifact, most likely due to overzealous pathologists. What is everyone else using? Do you use the canned spray? Thanks Crystal Morris M.T.(ASCP) Anatomical Supervisor Fairfield Medical Center Laboratory 401 N. Ewing St. Lancaster, Ohio 43130 (740) 687-8807 "Confidentiality Notice: This e-mail message, including any attachments is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review; use; disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message." ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Tue Jul 10 17:03:02 2007 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Jul 10 17:03:13 2007 Subject: [Histonet] Cell Block Procedure?? Message-ID: The following notes might be of help: Fibrin Clot Method Reference: Furtado (1970) Principle: The Thromboplastin cell-block is very gentle on cells and allows a wide range of routine immunohistochemical stains to be done. Plasma, treated with EDTA, is mixed with the specimen in the presence of thromboplastin and calcium ions to form a cell clot. This method is not suitable for cells fixed in formalin or specimens of bile fluid (bile enzymes prevent clot formation). In these instances the agar cell block method is preferred. Solutions: 1. Normal Plasma, containing EDTA, use out of date plasma obtained from Blood Bank. Freeze in 2ml aliquots. This plasma has been tested for known viruses and is considered safe. 2. Thromboplastin, use "out of date" thromboplastin obtained from Haematology. 3. 1% calcium chloride 4. 10% formalin Method: 1. Spin fluid using a centrifuge. 2. Decant off supernatant 3. Resuspend pellet in 3 drops of plasma. 4. Add 3 drops of thromboplastin, mix 5. Add 3 drops of Calcium Chloride, mix gently 6. Allow to stand undisturbed for 15-20 minutes 7. Add 5-8ml 10% formalin and allow to fix 8. Place in a labelled tissue cassette and process as usual. Egg Albumin Cell Block Method Reference: Yamamoto et al (1985) Principle: In this method, egg albumin coagulates in the presence of ethanol to form a cellblock. Solutions: 1. Egg albumin stock: Dissolve 1g of Egg albumin in a minimum of glycerol (about 1/10 V/V). 2. 95% ethanol Procedure: 1. Centrifuge cell suspension and decant supernatant. 2. Add 250-500ul Egg albumin stock and mix gently. 3. Add an equal volume of 95% ethanol and allow to gel. 4. Add 5ml fixative and allow to fix for 4-24 hours. 5. Process as usual. Agar Cell Block Method Reference: Olson et al (1986) Solutions: 1. 3% Agar 2. Fixative Procedure: 1. Fix material in preferred fixative, 50% ethanol 30min. 2. Heat 3% agar in an oven until molten. 3. Centrifuge specimen and decant supernatant. 4. Add 0.5ml molten agar 5. Allow to cool, add fixative, remove block and process. Mini-processing Cell Block Method Reference: Krogerus & Andersson (1988) Principle: This is a mini-processing method where cell centrifugation and processing is undertaken in the same centrifuge tube. The xylene step is omitted, being replaced by acetone and drying. Acetone evaporates rapidly at 60oC while fast drying is not achieved with xylene. Samples containing abundant mucus cause some problems since these were not efficiently infiltrated by paraffin. Solutions: 1. Fixative Acetone 900ml Ethanol 100ml Trichloroacetic acid 0.5ml or 10% buffered formalin or 50% ethanol can be used. 2. Acetone 3. Melted paraffin Procedure: 1. Fix cell pellet in fixative for at least one hour. 2. Centrifuge cell suspension and decant supernatant. 3. Add acetone, mix gently and leave for 10min. 4. Centrifuge specimen; remove supernatant and dry tubes in 60oC oven for one hour. 5. Add melted paraffin to the warmed cell pellets, gently mix, centrifuge and leave tube to cool to room temperature. 6. Remove paraffin cellblock by gently tapping the bottom of the tube. The blocks are now ready to section. Notes: A similar technique to the above has been reported by Domagala et al (1990) Specific Processing Method for Cell Blocks Reference: Kung et al (1989) Principle: Often the cytological preservation in cellblock sections was far inferior to that of surgical specimens fixed and processed according to the standard histological processing schedule. The cells appear shrunken, nuclei were small and dark rendering assessment of chromatin pattern difficult, and the nucleoli were less conspicuous. The following technique uses a shorter time in xylene and a more gradual change over from alcohol. The use of 7.5% rather than 10% formalin is also recommended. Procedure: 1. 7.5% formalin 6-12hrs. 2. 70% ethanol 30min. 3. 95% ethanol 30min. 4. Absolute ethanol 1hr. 5. Absolute ethanol 1hr. 6. Absolute ethanol 1hr. 7. Ethanol: xylene 1hr. 8. Xylene 1hr. 9. Xylene 1hr. 10. Wax 2hr. 11. Wax 3 1/2 hr. OCT Cell Block Method Reference: Arisio (1989) Solutions: 1. Methacarn fixative Methanol 75ml Chloroform 20ml Acetic acid 5ml 2. OCT Compound 4583 (Miles) Procedure: 1. Centrifuge specimen and add 10ml methacarn fixative. 2. Mix and allow fixing for 1-2min. 3. Add 0.5-1ml OCT compound to the fixative. 4. Cap the tube and gently agitate the tube until the OCT clots. 5. Centrifuge the tube, remove the supernatant and add fresh fixative. 6. Process the cellblock as usual. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MICHELLE SEAGLE Sent: Wednesday, 11 July 2007 12:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cell Block Procedure?? Our Histo Lab is currently in the process of starting to do cell blocks. We would be interested in knowing about any procedures that you may like to share with us about doing these. We are interested in knowing the products and companys that you use to perform these as well as the procedure. Any info that you may be able to share with uswould be greatly appreciated. Thanks, Michelle Seagle HT(ASCP) Rutherford Hospital --------------------------------- Don't pick lemons. See all the new 2007 cars at Yahoo! Autos. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From caligirl004 <@t> hotmail.com Tue Jul 10 17:19:00 2007 From: caligirl004 <@t> hotmail.com (Victoria Socin) Date: Tue Jul 10 17:19:07 2007 Subject: [Histonet] Greensboro, NC Message-ID: Hi, I'm moving to the Greensboro, Nc. area in the next 2 months, and am looking for Histology labs in and around the Greensboro area. If anyone knows of some labs, please let me know! Thank You so much! Victoria _________________________________________________________________ http://liveearth.msn.com From mtarango <@t> nvcancer.org Tue Jul 10 18:58:02 2007 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Tue Jul 10 18:58:28 2007 Subject: [Histonet] CISH reagent kits In-Reply-To: Message-ID: <5AEC610C1CE02945BD63A395BA763EDE011B6D25@NVCIEXCH02.NVCI.org> Why not just buy what you need and do it yourself, I mean if its animal tissue... finding an A-Z type of kit might be hard. What probes are you looking to offer? I like lots of the things that BIOCARE Medical sells. You might want to check out their kinetic stainer, I don't have one, only because I bought something else before I heard about theirs. You might be able keep it easy by using FITC conjugated probes, perform FISH as usual and use an anti-FITC antibody /IHC for demonstration. If you aren't already performing FISH, maybe I should give a more detailed sort of response. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Woodward, Denise Sent: Thursday, July 05, 2007 8:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CISH reagent kits Hello all, We are looking to add C-ISH to our Veterinary Diagnostic Service and would like to know what C-ISH kits people are using out in the Clinical diagnostic world. Surprisingly, the vendors out there have been less than helpful with information about their products. I don't know how these people stay in business! Thanks, Denise Long Woodward, MS, HT (ASCP), HTL, QIHC Connecticut Veterinary Medical Diagnostic Laboratory University of Connecticut Dept. of Pathobiology and Veterinary Sciences 61 N. Eagleville Road Storrs, CT 06269-3089 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From arvidsonkristen <@t> yahoo.com Tue Jul 10 19:29:50 2007 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Tue Jul 10 19:29:57 2007 Subject: [Histonet] looking fo lab assistant info Message-ID: <548450.82554.qm@web61315.mail.yahoo.com> Hello All, Does anyone have Lab Assistant job description and qualification info? We are looking into hiring for this position and we need more info. Thanks! -Kristen --------------------------------- Yahoo! oneSearch: Finally, mobile search that gives answers, not web links. From carl.hobbs <@t> kcl.ac.uk Wed Jul 11 07:27:05 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Wed Jul 11 07:26:54 2007 Subject: [Histonet] re: anyone doing IHC for neural migration? LAMP? Message-ID: Not as such but I immunostain migratory progenitor neurones in postnatal rat/mouse brains in FFPW sections. I use anti Drebrin , which is a great marker for these cells. I've never heard of LAMP ( but have checked it out, in the Ab data sheets of various Ab Suppliers); is it highly expressed in normal, migratory cells? Your info on LAMP would be much appreciated. Carl Histology Manager, Wolfson CARD Kings College London Guys Campus London SE1 1UL England From asmith <@t> mail.barry.edu Wed Jul 11 08:27:44 2007 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Wed Jul 11 08:27:49 2007 Subject: [Histonet] glassware cleaner In-Reply-To: <20070710164124.E3D9E1D3B@courageux.cnchost.com> Message-ID: <7DBCCC1FBC77C94F99F920D0CA6400B61E40B9@exchsrv01.barrynet.barry.edu> The choice of cleaners usually depends on what is being cleaned off the glassware. The best remover of silver deposits is nitric acid, which is toxic, corrosive, and slightly volatile: you will need a fume hood, gloves, and a neoprene or chloroprene apron. Citranox or Eradistain are good for removing the last traces of basic dyes: they are as close to non-toxic as cleaning agents get. Hydrochloric acid will also remove basic dyes, but it is quite volatile and requires a good fume hood. Most acid dye residues wash out with water. The few that don't can be removed with Alconox. Alconox is also good for removing traces of protein. Alconox is only slightly toxic. For really stubborn organic residues, chromic acid is the cleaner of last resort. Chromic acid is a 5% solution of chromium trioxide or sodium dichromate in concentrated sulfuric acid. It is frightfully corrosive (gloves and apron!) and toxic as hell! Even the residues of chromic acid are toxic and must be SLOWLY neutralized with sodium hydroxide and saved for a waste hauler. (If you neutralize it too fast, the heat will break the container.) Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Tuesday, July 10, 2007 12:41 PM To: histonet@pathology.swmed.edu Subject: [Histonet] glassware cleaner I would appreciate if anyone could recommend an excellent cleaning solution for glassware and plastics, especially for silver (gms) stained glassware. If there is a non-toxic brand available, that would be fantastic. Thank you, Paula Lucas Bio-Path Medical Group _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From kmerriam2003 <@t> yahoo.com Wed Jul 11 08:33:24 2007 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Wed Jul 11 08:33:29 2007 Subject: [Histonet] Tracheal Whole Mounts Message-ID: <714847.2712.qm@web50303.mail.re2.yahoo.com> Hello everyone, I have been asked to do some IHC staining on mouse tracheal whole mounts. I was wondering if anyone has done this; I am looking for information on how to flatten out the trachea once it is removed from the animal. Do you think I should fix it first and then flatten it or fix and flatten at the same time. Any thoughts or suggestions on how to flatten a mouse trachea would be greatly appreciated. Thanks, Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA ____________________________________________________________________________________ 8:00? 8:25? 8:40? Find a flick in no time with the Yahoo! Search movie showtime shortcut. http://tools.search.yahoo.com/shortcuts/#news From KHilburn <@t> compucyte.com Wed Jul 11 09:02:31 2007 From: KHilburn <@t> compucyte.com (Kate Hilburn) Date: Wed Jul 11 09:01:56 2007 Subject: [Histonet] Quantitative Imaging Cytometry Seminar in Cambridge, UK Message-ID: I want to inform you of the symposium devoted to Quantitative Imaging Cytometry on the afternoon of Wednesday, July 18 at the Cambridge Research Institute of Cancer Research UK in Cambridge, England. The registration deadline is Thursday, July 12 and cannot be extended, as the Cancer Research Institute in Cambridge is a secure facility, and arrangements to allow access to the building must be put in place in advance. No last-minute registration requests can be honored. Scheduled presentations include: * "Laser Scanning Cytometry Technology for Life Sciences and Drug Discovery" (with focus on cell-based applications, e.g., cell cycle, apoptosis, high-content pre-clinical safety and toxicology assays, rare cell analysis, immunophenotyping, etc.) - Ed Luther, Principal Scientist, CompuCyte Corporation * "LSC analysis of tissue microarrays: Application to subcellular localization of p27 and prostate cancer recurrence" - Peter Gann, Director, Department of Pathology, College of Medicine, University of Illinois at Chicago, USA * "Use of LSC technology in the department of ultrastructural pathology" - David Krull, Senior Scientist, GlaxoSmithKline, NC, USA A demonstration of the iCys(r) Research Imaging Cytometer equipped with three lasers (Violet 405nm, Blue 488nm, Red 633nm), 3PMTs, laser scatter and laser light loss detectors and confocal option based on Yokogawa spinning disk technology will be offered by appointment at the Microscopy and Imaging facility of Cancer Research UK, Cambridge Research Institute, Robinson Way, Cambridge CB2 0RE. This will be an excellent opportunity to learn from the most advanced users of Quantitative Imaging Cytometry technology in academic institutions and pharmaceutical companies. The meeting and demonstration are free of charge. Please register at http://www.compucyte.com/RegistrationForm-UK-2007.htm by Thursday, July 12. More information is available at www.compucyte.com . From kerry.l.crabb <@t> gsk.com Wed Jul 11 09:23:17 2007 From: kerry.l.crabb <@t> gsk.com (kerry.l.crabb@gsk.com) Date: Wed Jul 11 09:23:41 2007 Subject: [Histonet] Histology Position Message-ID: GlaxoSmithKline has an open position in our Molecular and Ultrastructure Pathology (MUP) group. If interested, you will need to apply on-line at the GSK web site (www.gsk.com). Job Title: Scientist/Associate Scientist The position basic qualifications include: Bachelor's degree in a related science and a minimum of 3 years histotechnology experience Knowledge and experience in immunohistochemical, molecular pathology, routine histotechnology and/or electron microscopic procedures Knowledge and experience with Standard Operating Procedures and Good Laboratory Practice regulation and guidelines Knowledge and experience in instrument use and maintenance (including automated IHC stainers) Knowledge and experience in lab safety, occupational health, and environmental protection policies and regulations Detail and accuracy oriented with high quality standards in work performance Strong written and oral communication skills Very good teamwork and interpersonal relationship skills High standards of personal integrity and performance Preferred Qualifications include: HT(ASCP), HTL(ASCP) and/or QIHC(ASCP) certification desirable Experience in development of IHC or other molecular pathology methods/techniques desirable This person will: Perform IHC, molecular pathology, routine histotechnology and/or EM Perform work in a quality and quantity to meet Safety Assessment, Pathology, and MUP group requirements for report deadlines Make observations, enter/record findings and review data in electronic and/or written form to meet protocol, SOP, GLP and pathology requirements Participate in histotechnology, multidisciplinary teams (cross-site, or within SA) and in meeting with Pathology, MUP group and other SA co-workers Ensure facilities, equipment, and reagents are maintained and used in a safe manner and administered in accordance with applicable safety, occupational health and environmental protection guidelines, policies and regulations Maintain up-to-date knowledge of lab techniques, lab safety, and Good Laboratory Practices You may want to check out our web site for additional information. The GSK web site access for the job posting is: www.gsk.com Click Careers at GSK under the Quick Links (right side) in the blue section part way down the page Click US under the Careers section on the left side of the new window that opens Click Search and Apply for Jobs in the new window that opens Click Search for Jobs (left side) in a new window that opens Enter Requisition # 44125 in the box titled Req ID at the bottom of the new window and click search The link to the job position will appear in a new window. Just clink on the job listing (Scientist/Associate Scientist) The job posting will appear along with a box that allows you to apply. From christiegowan <@t> msn.com Wed Jul 11 09:51:37 2007 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Wed Jul 11 09:51:45 2007 Subject: [Histonet] Job openings in Birmingham, AL Message-ID: We have two histology openings at the University of Alabama Medical Center. The jobs are full time 40 hour week positions. One job is in the routine histology lab and the other is in the IHC lab. You can apply directly online at www.uab.edu or if you would like more info about the positions you can contact me at cgowan@uabmc.edu or call me at 205 934 4991. Birmingham is a beautiful city and working for the UAB health system opens the doors for many possibilities. Our salaries are competitive and there is opportunity for growth. We look forward to hearing from you! Christie Gowan HT (ASCP) UABMC Surgical Pathology Histology Supervisor Birmingham, AL cgowan@uabmc.edu 205 934 4991 From mlm11 <@t> cornell.edu Wed Jul 11 10:40:19 2007 From: mlm11 <@t> cornell.edu (Mary Lou Norman) Date: Wed Jul 11 10:40:24 2007 Subject: [Histonet] auto technicon Message-ID: <6.2.1.2.2.20070711113703.0482ff68@postoffice9.mail.cornell.edu> Hi Histonet, I'm back using the tried and true but I need directions. I have plenty of cards. The model number is 2A. Serial number is 6615. Thanks for all the help. fax 607-253-3435 Mary Lou From dahmed <@t> mdanderson.org Wed Jul 11 10:55:41 2007 From: dahmed <@t> mdanderson.org (dahmed@mdanderson.org) Date: Wed Jul 11 11:53:51 2007 Subject: [Histonet] Automated Special Stainers Message-ID: Anyone have recommendations on automated special stainers for the laboratory? David S. Ahmed, HT(ASCP) Supervisor, Clinical Histology Laboratory Department of Pathology M.D. Anderson Cancer Center From jcline <@t> wchsys.org Wed Jul 11 12:06:07 2007 From: jcline <@t> wchsys.org (Joyce Cline) Date: Wed Jul 11 12:06:15 2007 Subject: [Histonet] Geimsa using Dif Quik Message-ID: <000201c7c3dd$c5fd9eb0$1d2a14ac@wchsys.org> I would like to know the staining times used on Dif Quik for a Geimsa special stain. We currently use 15 seconds for each solution. I would like to tweak this and was wondering what everyone else was doing. ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From rjr6 <@t> psu.edu Wed Jul 11 12:17:23 2007 From: rjr6 <@t> psu.edu (Roberta Horner) Date: Wed Jul 11 12:17:28 2007 Subject: [Histonet] Geimsa using Dif Quik In-Reply-To: <000201c7c3dd$c5fd9eb0$1d2a14ac@wchsys.org> References: <000201c7c3dd$c5fd9eb0$1d2a14ac@wchsys.org> Message-ID: My Dif Quik says 5 dips for each solution and that is what I do. Roberta Horner HT/HTL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Wednesday, July 11, 2007 1:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Geimsa using Dif Quik I would like to know the staining times used on Dif Quik for a Geimsa special stain. We currently use 15 seconds for each solution. I would like to tweak this and was wondering what everyone else was doing. ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpaveli1 <@t> hurleymc.com Wed Jul 11 12:22:13 2007 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Wed Jul 11 12:23:21 2007 Subject: [Histonet] Geimsa using Dif Quik Message-ID: <4694D985020000EE00015FD9@smtp-gw.hurleymc.com> Hi Joyce, Our doctors like 2 minutes in the pink and 10 minutes in the Giemsa (no rinsing inbetween). Then we rinse for about 10 seconds in distilled water, air dry and coverslip. P.S. Watch for fungus growing in the solutions. It always shows up before the expiration date. We've added a thymol granule to each as a preservative. hope this helped, Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810-257-9948 fax: 810-762-7082 >>> "Joyce Cline" 07/11/07 1:06 PM >>> I would like to know the staining times used on Dif Quik for a Geimsa special stain. We currently use 15 seconds for each solution. I would like to tweak this and was wondering what everyone else was doing. ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Wed Jul 11 12:49:48 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Jul 11 12:49:46 2007 Subject: [Histonet] lab assistant looking for work in Portland, OR In-Reply-To: <548450.82554.qm@web61315.mail.yahoo.com> Message-ID: <002f01c7c3e3$e0b6fe30$6501a8c0@Patsy> One of my students trained as a lab assistant in my Histology lab is moving to Portland, OR and looking for a job. She has an AA degree and is planning on continuing school to get her bachelor's degree. She is trained in tissue processing, embedding, accessioning, general lab chemistry for making reagents, the use of a ph meter and balance, pipetting, etc. She is very computer literate and does all of our inventory organizing using spread sheets and general IT work. Best regards, Patsy Ruegg IHCtech 12635 Montview Blvd.Ste.215 Aurora, CO 80045 720-859-4060 pruegg@ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kristen arvidson Sent: Tuesday, July 10, 2007 6:30 PM To: histonet Subject: [Histonet] looking fo lab assistant info Hello All, Does anyone have Lab Assistant job description and qualification info? We are looking into hiring for this position and we need more info. Thanks! -Kristen --------------------------------- Yahoo! oneSearch: Finally, mobile search that gives answers, not web links. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Wed Jul 11 13:00:10 2007 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Wed Jul 11 13:00:22 2007 Subject: [Histonet] Automated Special Stainers In-Reply-To: Message-ID: David, The best special stainer that I know of is the Artisan sold by Dako. Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of dahmed@mdanderson.org Sent: Wednesday, July 11, 2007 10:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automated Special Stainers Anyone have recommendations on automated special stainers for the laboratory? David S. Ahmed, HT(ASCP) Supervisor, Clinical Histology Laboratory Department of Pathology M.D. Anderson Cancer Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NMargaryan <@t> childrensmemorial.org Wed Jul 11 14:35:51 2007 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Wed Jul 11 14:35:19 2007 Subject: [Histonet] process of the ISH In-Reply-To: References: Message-ID: Hello all, We are looking for a lab in/near Chicago who could kindly show/teach me whole process of the ISH. We are thinking to do in situ hybridization on mouse tissue with human tumor in (the Melanoma model). The probes (non-radioactive) will be house made. Hope to hear as soon as possible. Regards, Naira From kmilne <@t> bccancer.bc.ca Wed Jul 11 14:40:29 2007 From: kmilne <@t> bccancer.bc.ca (Milne, Katy) Date: Wed Jul 11 14:40:35 2007 Subject: [Histonet] Transferring tissue from OCT to NBF Message-ID: <07979E76B0869D4E8C9FE4AA9FC06578046688FD@srvex03.phsabc.ehcnet.ca> Hi everyone. We have some extra ovarian tumour samples frozen in OCT that we would like to transfer to formalin so that we can then process and use as part of a TMA. I'm just wondering if there are any special protocols that should be followed or if we're ok with just trimming off excess OCT then rinsing in NBF and fixing O/N in NBF? Thanks, Katy Milne Deeley Research Centre BC Cancer Agency From JGREWE <@t> OhioHealth.com Wed Jul 11 15:05:40 2007 From: JGREWE <@t> OhioHealth.com (JGREWE@OhioHealth.com) Date: Wed Jul 11 15:05:51 2007 Subject: [Histonet] Jacquelyn Grewe/Staff/OhioHealth is out of the office . Message-ID: I will be out of the office starting 07/11/2007 and will not return until 07/22/2007. I will respond to your message when I return. Thanks, Jackie From Erin.Martin <@t> ucsf.edu Wed Jul 11 15:32:03 2007 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Wed Jul 11 15:32:56 2007 Subject: [Histonet] Re: looking for lab assistant info Message-ID: Hi Kristen, I think that would depend on what kind of work you want them to do - "Lab assistant" in my experience is a catch all title that varies greatly from hospital to hospital. I have worked at labs where they were limited to filing slides and stocking supplies, and at others where they did cyto prep, stain maintenance, specimen accessioning, etc, and one where they did tissue grossing. It really depends where you are and how your job titles work; a union shop would have strict rules (last place I worked the title "lab assistant" meant licensed phlebotomist under the contract). The more complicated the work, the more education/experience they would probably need. Good luck, Erin Martin Hello All, Does anyone have Lab Assistant job description and qualification info? We are looking into hiring for this position and we need more info. Thanks! -Kristen From innvx <@t> sbcglobal.net Wed Jul 11 15:39:18 2007 From: innvx <@t> sbcglobal.net (INNOVEX BIOSCIENCES) Date: Wed Jul 11 15:39:23 2007 Subject: [Histonet] Stat Animal IHC Workshop/San Francisco Message-ID: <591216.78868.qm@web82008.mail.mud.yahoo.com> Stat Animal IHC workshop will be held in San Francisco on Aug 17th, 1-4:30 PM. A few spaces are still available for this hands-on wet workshop that aims at teaching methods of background -free animal IHC and Mouse-on-mouse IHC in less than 2 hours. This workshop is CEU approved. A rare opportunity for northern California and adjoining states, this workshop will not be given in California for some time. Call 1-800-622-7808 or innvx@sbcglobal.net or innvx.com ABSTRACT: This is a comprehensive hands-on wet workshop designed to address the major issues associated with IHC staining of animal tissues. The topics covered will include methods for eradication of background staining in animal IHC, the techniques for lowering the primary antibody incubation time and decreasing the IHC staining run to under 2 hours. This workshop will further provide helpful information for background-free staining of identical species primaries- and-identical species tissues such as Mouse-On-Mouse, Rabbit-On-Rabbit, Mouse-On-Rat, etc. Other topics covered will include the selection of appropriate secondary antibodies and Co-reagents for animal IHC staining and also novel Room temperature amplification techniques for maximizing optimal staining and eliminating troubleshooting. The application of animal STAT-IHC system for both manual staining and automated stainers will be explored. This workshop is CEU approved. From rjbuesa <@t> yahoo.com Wed Jul 11 15:55:59 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jul 11 15:56:02 2007 Subject: [Histonet] Transferring tissue from OCT to NBF In-Reply-To: <07979E76B0869D4E8C9FE4AA9FC06578046688FD@srvex03.phsabc.ehcnet.ca> Message-ID: <565809.11681.qm@web61212.mail.yahoo.com> Trim the excess OCT and place it in NBF (to complete thawing). Leave in NBF as usual. Ren? J. "Milne, Katy" wrote: Hi everyone. We have some extra ovarian tumour samples frozen in OCT that we would like to transfer to formalin so that we can then process and use as part of a TMA. I'm just wondering if there are any special protocols that should be followed or if we're ok with just trimming off excess OCT then rinsing in NBF and fixing O/N in NBF? Thanks, Katy Milne Deeley Research Centre BC Cancer Agency _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Moody friends. Drama queens. Your life? Nope! - their life, your story. Play Sims Stories at Yahoo! Games. From ladylaynah <@t> yahoo.com Wed Jul 11 20:47:12 2007 From: ladylaynah <@t> yahoo.com (Constance McManus) Date: Wed Jul 11 20:47:15 2007 Subject: [Histonet] MYSTERY SOLVED!! Message-ID: <693063.67472.qm@web37002.mail.mud.yahoo.com> Well, gang, I posted a question some time ago about mysteriously skipping staining of MiTF IHC frozen sections. Some of you responded with some really good information, but no one could tell me why the stain skipped on some tissue and stained other tissue in the same block. I figured it out myself as I was checking my e-mail over breakfast one morning. In a word or two -- freeze-thaw and freeze again. During our embedding we were freezing the tissue to get the epidermis flat and as we worked the next piece of tissue, the first one thawed. Then we froze both tissues which caused freeze artifact. This is deadly to epitopes. I have always taken great care to keep our control tissues frozen (to the extent that I keep them encapsulated in OCT and carry them to the ultra low freezer in a LN2 cooled container) and I store the cut control slides in a -20C freezer, taking out only what I need. But I never noticed the freeze/thaw going on as we embedded our tissues. What can I say, except that I feel so much like a newbie. Anyway, thanks for the responses. Connie McManus, HT University of Utah School of Medicine Dept. of Dermatology ____________________________________________________________________________________ Bored stiff? Loosen up... Download and play hundreds of games for free on Yahoo! Games. http://games.yahoo.com/games/front From galinadeyneko <@t> yahoo.com Thu Jul 12 09:06:54 2007 From: galinadeyneko <@t> yahoo.com (Galina Deyneko) Date: Thu Jul 12 09:07:01 2007 Subject: [Histonet] CD 31 for rabbit Message-ID: <20070712140655.58852.qmail@web33103.mail.mud.yahoo.com> Bernice, CD 31 mouse anti-human Dako #M 0823 works great in my hands on FFPE and frozen rabbit's aorta. On frozen I do not use pre-treatment. My protocol: IHC protocol for rabbit???s tissues using CD31 and Polymer ???HRP. 1.De-wax and hydrate the slides. 2 Place slides in Tris Buffer 4. Preatretment with Proteinase K for 15 min. at RT W.Volume : 10 000??l) ?? (Promega # V3021) Concentration: 10mg/1ml (100mg is diluted in 10 ml of 1mM Tris (Ambion #9856,pH8.0) Final concentration: 50 ??g/1ml Preparation: 10000 ??g of concentration : 50 ??g of final concentration = 200 diluent factor. Dilution of Proteinase K 1:200. 3.000 ??l working volume : 200 = 15 ??l of concentrated Proteinase K + 2.985 ??L of 1 mM Tris Buffer. Preparation of 1mM of Tris Buffer: 1 ??l of 1M Tris Buffer + 999 ??l of D water 3 ??l of 1M Tris + 2.997 ??l of D water Optional: Proteinase K (Dako, cat.# S3004) 40 ??l ( 1 drop) of concentrated proteinase K mix with 4 ml of 0,05 M Tris, pH 8 (manufarcture instruction) 2 drops of concentrated proteinase K with 10 ml of 0,05 M Tris Preparation of 0,05 mol/L Tris: 1M Tris x X ??l = 0,05 M Tris x w.volume: 1M Tris x X ??l = 0,05 M Tris x 10 000 ??L X = 0,05M x 10 000 ??l : 1M = 500 ??l of 1M Tris + 9500 ??l of D water 5. Rinse with Tris Buffer 6. Rinse with Tris Buffer. 7. Block peroxidase activity with Peroxidazed 1 for 5 min. at RT??. (Biocare #PX968) 8. Rinse with Tris Buffer 9. Protein block with Background Sniper for 20 min at RT?? ( Biocare #BS966). 10. Rinse with Tris Buffer 10.Incubation with primary AB overnight at 4 C ( Monoclonal Mouse anti-Human CD 31, Endothelial cell, Dako Cytomation #M0823) Dilution 1:100 Diluent: Da Vincy Green, Biocare #PD900 8.500 ??l working volume :100 =85??l of AB 85??l of primary AB +8415 ??l of diluent. Day two 11. Rinse with Tris Buffer. 12. Incubation with MACH 2 Poly HRP Conjugate for 30 min. at RT?? (Biocare, # MHRP520). 13. Rinse twice with Tris Buffer 14.Developing with DAB for min at RT?? (Biocare # DB801,kit) 1000 ??l of Substrate Buffer + 50 ??l (1 drop) of Chromogen 15.Rinse with water. 16.Developing with DABSparkle for 2 min at RT?? (Biocare, # DS830) 17. Counterstain with Gill???s Hematoxylin dilution 1:5 (Poly Scientific, cat# s211)for min 18. Dehydrate, coverslip. Galina Deyneko Novartis ,Cambridge MA --------------------------------- The fish are biting. Get more visitors on your site using Yahoo! Search Marketing. From rneuenhoff <@t> yahoo.com Thu Jul 12 09:53:24 2007 From: rneuenhoff <@t> yahoo.com (Rachel Neuenhoff) Date: Thu Jul 12 09:53:29 2007 Subject: [Histonet] predentine Message-ID: <917741.56407.qm@web56503.mail.re3.yahoo.com> I am trying to visualize the predentine layer in teeth. It is made up of a matrix of phosphoprotein and glycosaminoglycans (chondroitin-6-sulfate) and scattered type 1 collagen fibers. I've tried H&E with mixed results. Does someone know of stains that are specific to all three of these? I've read that alcain blue may work. Thanks! Rachel Neuenhoff Texas A&M University at Galveston rneuenhoff@yahoo.com --------------------------------- Never miss an email again! Yahoo! Toolbar alerts you the instant new Mail arrives. Check it out. From jjohnson <@t> crispregional.org Thu Jul 12 10:19:46 2007 From: jjohnson <@t> crispregional.org (Jennifer Johnson) Date: Thu Jul 12 10:19:54 2007 Subject: [Histonet] Histotech position available Message-ID: <000001c7c498$14afff20$2082010a@main.crispregional.org> Histotech position available at Crisp Regional Hospital in Cordele, Georgia. Requirements: Bachelor's or Associate*s degree with strong relevant experience in Chemistry & Biology acceptable. Relevant pathology laboratory experience desired. Histology Technician or Technologist Certification desired, but not required. I have been the sole Histotech in the lab and I am leaving. If you need any information, directly reply to me. From mcauliff <@t> umdnj.edu Thu Jul 12 10:41:57 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Jul 12 10:42:51 2007 Subject: [Histonet] predentine In-Reply-To: <917741.56407.qm@web56503.mail.re3.yahoo.com> References: <917741.56407.qm@web56503.mail.re3.yahoo.com> Message-ID: <46964BC5.5040905@umdnj.edu> You could put Alcian Blue, Safranin O or Toluidine blue O in the fixative (yes, in the fixative) to improve retention of GAGs. There is no one stain that will demonstrate all connective tissue components. Geoff Rachel Neuenhoff wrote: > I am trying to visualize the predentine layer in teeth. It is made up of a matrix of phosphoprotein and glycosaminoglycans (chondroitin-6-sulfate) and scattered type 1 collagen fibers. I've tried H&E with mixed results. Does someone know of stains that are specific to all three of these? I've read that alcain blue may work. Thanks! > > Rachel Neuenhoff > Texas A&M University at Galveston > rneuenhoff@yahoo.com > > > --------------------------------- > Never miss an email again! > Yahoo! Toolbar alerts you the instant new Mail arrives. Check it out. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From lesley.bechtold <@t> jax.org Thu Jul 12 11:00:32 2007 From: lesley.bechtold <@t> jax.org (Lesley Bechtold) Date: Thu Jul 12 11:02:25 2007 Subject: [Histonet] Clinical cryostat info needed Message-ID: <20070712120032635.00000005664@spikey> Hi, I need to provide information and approximate pricing for a clinical cryostat - one with a decontamination system. I would welcome responses from vendors but any info on the best model out there - particularly any models that are user-friendly for a wide range of potential users - would be welcome. Thank you Lesley Lesley S. Bechtold Senior Manager, Histopathology & Microscopy Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 From cmiller <@t> physlab.com Thu Jul 12 11:25:07 2007 From: cmiller <@t> physlab.com (Cheri Miller) Date: Thu Jul 12 11:25:15 2007 Subject: [Histonet] Automated Special Stainers In-Reply-To: References: Message-ID: <000f01c7c4a1$37b97bf0$3402a8c0@plab.local> Big fan of the Ventana here Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of dahmed@mdanderson.org Sent: Wednesday, July 11, 2007 10:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automated Special Stainers Anyone have recommendations on automated special stainers for the laboratory? David S. Ahmed, HT(ASCP) Supervisor, Clinical Histology Laboratory Department of Pathology M.D. Anderson Cancer Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From gcallis <@t> montana.edu Thu Jul 12 11:25:34 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Jul 12 11:25:22 2007 Subject: [Histonet] Predentine staining, a publication Message-ID: <6.0.0.22.1.20070712102106.01b44e40@gemini.msu.montana.edu> If you have access to Calcified Tissue Internation, this publication may be of help. An oldie but looks like a good article. Mallory triple, alcian blue and other stains are discussed. Everett MM and Miller WA. Histochemical studies on calcified tissues. II. amino acid histochemistry on developing dentine and bone. Calcificied Tissue International 16(1):73-88, 1974 Also, contact "Barry R Rittman" , he is an expert on teeth. He may even have this article on file. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From jmahoney <@t> alegent.org Thu Jul 12 11:42:12 2007 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Thu Jul 12 11:42:30 2007 Subject: [Histonet] Automated Special Stainers In-Reply-To: <000f01c7c4a1$37b97bf0$3402a8c0@plab.local> References: <000f01c7c4a1$37b97bf0$3402a8c0@plab.local> Message-ID: <469613940200003C0001388A@gwia.alegent.org> Ventana for me too. Jan Alegent Health, Omaha NE >>> "Cheri Miller" 07/12/2007 11:25 AM >>> Big fan of the Ventana here Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of dahmed@mdanderson.org Sent: Wednesday, July 11, 2007 10:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automated Special Stainers Anyone have recommendations on automated special stainers for the laboratory? David S. Ahmed, HT(ASCP) Supervisor, Clinical Histology Laboratory Department of Pathology M.D. Anderson Cancer Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Thu Jul 12 12:00:27 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Jul 12 12:00:38 2007 Subject: [Histonet] Automated Special Stainers In-Reply-To: <469613940200003C0001388A@gwia.alegent.org> Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C669@IS-E2K3.grhs.net> Ventana here also! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: Thursday, July 12, 2007 11:42 AM To: histonet@lists.utsouthwestern.edu; dahmed@mdanderson.org; Cheri Miller Subject: RE: [Histonet] Automated Special Stainers Ventana for me too. Jan Alegent Health, Omaha NE >>> "Cheri Miller" 07/12/2007 11:25 AM >>> Big fan of the Ventana here Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of dahmed@mdanderson.org Sent: Wednesday, July 11, 2007 10:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automated Special Stainers Anyone have recommendations on automated special stainers for the laboratory? David S. Ahmed, HT(ASCP) Supervisor, Clinical Histology Laboratory Department of Pathology M.D. Anderson Cancer Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HoustonR <@t> chi.osu.edu Thu Jul 12 12:16:35 2007 From: HoustonR <@t> chi.osu.edu (Houston, Ronald) Date: Thu Jul 12 12:17:40 2007 Subject: [Histonet] Automated Special Stainers In-Reply-To: <661949901A768E4F9CC16D8AF8F2838CA1C669@IS-E2K3.grhs.net> Message-ID: <979FF5962E234F45B06CF0DB7C1AABB210BC8024@chi2k3ms01.columbuschildrens.net> Never in a month of Sundays; Artisan is head and shoulders better Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Columbus Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 houstonr@chi.osu.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Thursday, July 12, 2007 1:00 PM To: Janice Mahoney; histonet@lists.utsouthwestern.edu; dahmed@mdanderson.org; Cheri Miller Subject: RE: [Histonet] Automated Special Stainers Ventana here also! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: Thursday, July 12, 2007 11:42 AM To: histonet@lists.utsouthwestern.edu; dahmed@mdanderson.org; Cheri Miller Subject: RE: [Histonet] Automated Special Stainers Ventana for me too. Jan Alegent Health, Omaha NE >>> "Cheri Miller" 07/12/2007 11:25 AM >>> Big fan of the Ventana here Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of dahmed@mdanderson.org Sent: Wednesday, July 11, 2007 10:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automated Special Stainers Anyone have recommendations on automated special stainers for the laboratory? David S. Ahmed, HT(ASCP) Supervisor, Clinical Histology Laboratory Department of Pathology M.D. Anderson Cancer Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: This e-mail message, from Children's Hospital, Columbus, Ohio, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes pursuant to Children's Hospital's confidentiality policies. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From BFicher <@t> chomp.org Thu Jul 12 12:28:45 2007 From: BFicher <@t> chomp.org (Fischer, R. B) Date: Thu Jul 12 12:28:54 2007 Subject: [Histonet] Microwave processing References: Message-ID: I have a question regarding IHC staining using tissues from a microwave processor. I recently attended an IHC workshop at Ventana. and was surprised to learn that IHC staining should not be carried out on any microwaved processed tissues. I have been pro microwave processing since they hit the marketplace, and am anxious to purchase one, but the info not to stain IHC from one has me worried. Can anyone share info in support of this statement? Thanks, R. Brian Fischer Histology Lead Tech Community Hospital of the Monterey Peninsula PO Box H.H. Monterey, Ca. 93942 831-625-4791 Fax: 831-625-4793 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Bartlett, Jeanine Sent: Wed 2/22/2006 3:12 PM To: John PJ Coleman; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Microwave processing We have the Sakura Xpress and we are still doing side-by-side comparisons on a variey of tissue types, IHC testing and special stains. So far the tissues form the Xpress have been as good as, and is some cases superior to, the traditional processing method. Jeanine Bartlett CDC Atlanta -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of John PJ Coleman Sent: Wed 2/22/2006 5:04 PM To: histonet@lists.utsouthwestern.edu Cc: Subject: [Histonet] Microwave processing I am the Senior tech of a large hospital corporation. My administration has just won funding for 4 Sakura Microwave rapid processing units. We run FISH her 2 on formalin fixed paraffin embedded tissue as per FDA protocol. As I tech, I am not in favor of tossing routine processing wholesale in favor of a completely new technology without thorough testing and parallel processing. Also, we are a regional reference lab for IHC and have a panel of 115 antibodies, all optimized for formalin fixed paraffin embedded tissue. We run an average of 350 IHC slides a day, max 580 per day and would have to re-optimize these to use in the new formalin free world while keeping our FFPE procedures in parallel for our reference lab work. Much like running 2 labs. If anyone has any insight, or if anyone currently uses these instruments for routine and/or IHC, feel free to call or email, and I'll check the postings on this string. We are also taking invitations to come out and see these things in use real time. John PJ Coleman-757 335-2159 http://members.cox.net/captainmadman/ http://journals.aol.com/jcolclefa/Waytoomuchboringcraptosayusually/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This is a transmission from Community Hospital of the Monterey Peninsula. This message and any attached documents may be confidential and contain information protected by state and federal medical privacy statutes. They are intended only for the use of the addressee. If you are not the intended recipient, any disclosure, copying, or distribution of this information is strictly prohibited. If you received this transmission in error, please accept our apologies and notify the sender. Thank you. From jqb7 <@t> CDC.GOV Thu Jul 12 12:33:15 2007 From: jqb7 <@t> CDC.GOV (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Thu Jul 12 12:37:00 2007 Subject: [Histonet] Microwave processing References: Message-ID: <34BB307EFC9A65429BBB49E330675F7201B0AAD8@LTA3VS003.ees.hhs.gov> Brian, We have the Sakura Xpress and we performed side-by-side testing (IHC, specials and H&Es) of traditionally processed tissues and Xpress processed tissues. The only difference we noted was slightly crisper IHC staining with the Xpress over the traditional processor. Jeanine Bartlett CDC Atlanta ________________________________ From: Fischer, R. B [mailto:BFicher@chomp.org] Sent: Thu 7/12/2007 1:28 PM To: Bartlett, Jeanine (CDC/CCID/NCZVED); John PJ Coleman; histonet@lists.utsouthwestern.edu Cc: Delcambre, Linda V; Keating, Jeffrey - MD Subject: RE: [Histonet] Microwave processing I have a question regarding IHC staining using tissues from a microwave processor. I recently attended an IHC workshop at Ventana. and was surprised to learn that IHC staining should not be carried out on any microwaved processed tissues. I have been pro microwave processing since they hit the marketplace, and am anxious to purchase one, but the info not to stain IHC from one has me worried. Can anyone share info in support of this statement? Thanks, R. Brian Fischer Histology Lead Tech Community Hospital of the Monterey Peninsula PO Box H.H. Monterey, Ca. 93942 831-625-4791 Fax: 831-625-4793 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Bartlett, Jeanine Sent: Wed 2/22/2006 3:12 PM To: John PJ Coleman; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Microwave processing We have the Sakura Xpress and we are still doing side-by-side comparisons on a variey of tissue types, IHC testing and special stains. So far the tissues form the Xpress have been as good as, and is some cases superior to, the traditional processing method. Jeanine Bartlett CDC Atlanta -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of John PJ Coleman Sent: Wed 2/22/2006 5:04 PM To: histonet@lists.utsouthwestern.edu Cc: Subject: [Histonet] Microwave processing I am the Senior tech of a large hospital corporation. My administration has just won funding for 4 Sakura Microwave rapid processing units. We run FISH her 2 on formalin fixed paraffin embedded tissue as per FDA protocol. As I tech, I am not in favor of tossing routine processing wholesale in favor of a completely new technology without thorough testing and parallel processing. Also, we are a regional reference lab for IHC and have a panel of 115 antibodies, all optimized for formalin fixed paraffin embedded tissue. We run an average of 350 IHC slides a day, max 580 per day and would have to re-optimize these to use in the new formalin free world while keeping our FFPE procedures in parallel for our reference lab work. Much like running 2 labs. If anyone has any insight, or if anyone currently uses these instruments for routine and/or IHC, feel free to call or email, and I'll check the postings on this string. We are also taking invitations to come out and see these things in use real time. John PJ Coleman-757 335-2159 http://members.cox.net/captainmadman/ http://journals.aol.com/jcolclefa/Waytoomuchboringcraptosayusually/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This is a transmission from Community Hospital of the Monterey Peninsula. This message and any attached documents may be confidential and contain information protected by state and federal medical privacy statutes. They are intended only for the use of the addressee. If you are not the intended recipient, any disclosure, copying, or distribution of this information is strictly prohibited. If you received this transmission in error, please accept our apologies and notify the sender. Thank you. From rjbuesa <@t> yahoo.com Thu Jul 12 12:48:57 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jul 12 12:49:01 2007 Subject: [Histonet] Microwave processing In-Reply-To: Message-ID: <544925.23270.qm@web61217.mail.yahoo.com> Theoretically there should be NO difference in IHC reactions on tissue processed using microwave technology versus "conventional" tissue processing. You should have asked Ventana why do they "issued" that theoretically invalid warning, what tests have they done to support it. Ren? J. "Fischer, R. B" wrote: I have a question regarding IHC staining using tissues from a microwave processor. I recently attended an IHC workshop at Ventana. and was surprised to learn that IHC staining should not be carried out on any microwaved processed tissues. I have been pro microwave processing since they hit the marketplace, and am anxious to purchase one, but the info not to stain IHC from one has me worried. Can anyone share info in support of this statement? Thanks, R. Brian Fischer Histology Lead Tech Community Hospital of the Monterey Peninsula PO Box H.H. Monterey, Ca. 93942 831-625-4791 Fax: 831-625-4793 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Bartlett, Jeanine Sent: Wed 2/22/2006 3:12 PM To: John PJ Coleman; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Microwave processing We have the Sakura Xpress and we are still doing side-by-side comparisons on a variey of tissue types, IHC testing and special stains. So far the tissues form the Xpress have been as good as, and is some cases superior to, the traditional processing method. Jeanine Bartlett CDC Atlanta -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of John PJ Coleman Sent: Wed 2/22/2006 5:04 PM To: histonet@lists.utsouthwestern.edu Cc: Subject: [Histonet] Microwave processing I am the Senior tech of a large hospital corporation. My administration has just won funding for 4 Sakura Microwave rapid processing units. We run FISH her 2 on formalin fixed paraffin embedded tissue as per FDA protocol. As I tech, I am not in favor of tossing routine processing wholesale in favor of a completely new technology without thorough testing and parallel processing. Also, we are a regional reference lab for IHC and have a panel of 115 antibodies, all optimized for formalin fixed paraffin embedded tissue. We run an average of 350 IHC slides a day, max 580 per day and would have to re-optimize these to use in the new formalin free world while keeping our FFPE procedures in parallel for our reference lab work. Much like running 2 labs. If anyone has any insight, or if anyone currently uses these instruments for routine and/or IHC, feel free to call or email, and I'll check the postings on this string. We are also taking invitations to come out and see these things in use real time. John PJ Coleman-757 335-2159 http://members.cox.net/captainmadman/ http://journals.aol.com/jcolclefa/Waytoomuchboringcraptosayusually/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This is a transmission from Community Hospital of the Monterey Peninsula. This message and any attached documents may be confidential and contain information protected by state and federal medical privacy statutes. They are intended only for the use of the addressee. If you are not the intended recipient, any disclosure, copying, or distribution of this information is strictly prohibited. If you received this transmission in error, please accept our apologies and notify the sender. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Building a website is a piece of cake. Yahoo! Small Business gives you all the tools to get online. From HoustonR <@t> chi.osu.edu Thu Jul 12 12:59:01 2007 From: HoustonR <@t> chi.osu.edu (Houston, Ronald) Date: Thu Jul 12 13:00:06 2007 Subject: [Histonet] Microwave processing In-Reply-To: <544925.23270.qm@web61217.mail.yahoo.com> Message-ID: <979FF5962E234F45B06CF0DB7C1AABB210BC807B@chi2k3ms01.columbuschildrens.net> I have never heard so much drivel; Ventana come out with some good ones every so often, but this takes the biscuit. Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Columbus Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 houstonr@chi.osu.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, July 12, 2007 1:49 PM To: Fischer, R. B; Bartlett, Jeanine; John PJ Coleman; histonet@lists.utsouthwestern.edu Cc: Keating, Jeffrey - MD; Delcambre,Linda V Subject: RE: [Histonet] Microwave processing Theoretically there should be NO difference in IHC reactions on tissue processed using microwave technology versus "conventional" tissue processing. You should have asked Ventana why do they "issued" that theoretically invalid warning, what tests have they done to support it. Ren? J. "Fischer, R. B" wrote: I have a question regarding IHC staining using tissues from a microwave processor. I recently attended an IHC workshop at Ventana. and was surprised to learn that IHC staining should not be carried out on any microwaved processed tissues. I have been pro microwave processing since they hit the marketplace, and am anxious to purchase one, but the info not to stain IHC from one has me worried. Can anyone share info in support of this statement? Thanks, R. Brian Fischer Histology Lead Tech Community Hospital of the Monterey Peninsula PO Box H.H. Monterey, Ca. 93942 831-625-4791 Fax: 831-625-4793 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Bartlett, Jeanine Sent: Wed 2/22/2006 3:12 PM To: John PJ Coleman; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Microwave processing We have the Sakura Xpress and we are still doing side-by-side comparisons on a variey of tissue types, IHC testing and special stains. So far the tissues form the Xpress have been as good as, and is some cases superior to, the traditional processing method. Jeanine Bartlett CDC Atlanta -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of John PJ Coleman Sent: Wed 2/22/2006 5:04 PM To: histonet@lists.utsouthwestern.edu Cc: Subject: [Histonet] Microwave processing I am the Senior tech of a large hospital corporation. My administration has just won funding for 4 Sakura Microwave rapid processing units. We run FISH her 2 on formalin fixed paraffin embedded tissue as per FDA protocol. As I tech, I am not in favor of tossing routine processing wholesale in favor of a completely new technology without thorough testing and parallel processing. Also, we are a regional reference lab for IHC and have a panel of 115 antibodies, all optimized for formalin fixed paraffin embedded tissue. We run an average of 350 IHC slides a day, max 580 per day and would have to re-optimize these to use in the new formalin free world while keeping our FFPE procedures in parallel for our reference lab work. Much like running 2 labs. If anyone has any insight, or if anyone currently uses these instruments for routine and/or IHC, feel free to call or email, and I'll check the postings on this string. We are also taking invitations to come out and see these things in use real time. John PJ Coleman-757 335-2159 http://members.cox.net/captainmadman/ http://journals.aol.com/jcolclefa/Waytoomuchboringcraptosayusually/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This is a transmission from Community Hospital of the Monterey Peninsula. This message and any attached documents may be confidential and contain information protected by state and federal medical privacy statutes. They are intended only for the use of the addressee. If you are not the intended recipient, any disclosure, copying, or distribution of this information is strictly prohibited. If you received this transmission in error, please accept our apologies and notify the sender. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Building a website is a piece of cake. Yahoo! Small Business gives you all the tools to get online. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: This e-mail message, from Children's Hospital, Columbus, Ohio, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes pursuant to Children's Hospital's confidentiality policies. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From mrsseagle <@t> yahoo.com Thu Jul 12 13:44:18 2007 From: mrsseagle <@t> yahoo.com (MICHELLE SEAGLE) Date: Thu Jul 12 13:44:21 2007 Subject: [Histonet] Xylene Substitutes??? Message-ID: <305373.14624.qm@web51808.mail.re2.yahoo.com> Has anyone had a good experience with any Xylene substitutes?? If so what kind are you using?? Thanks Michelle Seagle HT(ASCP) --------------------------------- Boardwalk for $500? In 2007? Ha! Play Monopoly Here and Now (it's updated for today's economy) at Yahoo! Games. From dshtilki <@t> uci.edu Thu Jul 12 13:46:56 2007 From: dshtilki <@t> uci.edu (Shtilkind, Dmitriy) Date: Thu Jul 12 13:47:14 2007 Subject: [Histonet] Van Gieson question.... Message-ID: <2937D5939ABCCA4F96C14BBF3E463A1C02C8EA2B@GUS.hs.uci.edu> You can try different kind of alcohol for mixing Hematoxyline. Pure ethanol is the best, but reagent alcohol also works. And don't forget to filter Hematoxylin + FeCl. Dmitriy Shtilkind, HTL (ASCP) Histology Supervisor UCI Medical Center Orange, CA, 92868 (714) 456-8731 dshtilki@uci.edu From gcallis <@t> montana.edu Thu Jul 12 13:54:02 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Jul 12 13:53:50 2007 Subject: [Histonet] RE: Microwave processing and IHC Message-ID: <6.0.0.22.1.20070712123706.01b1e560@gemini.msu.montana.edu> Could it be the Ventana workshop director was inexperienced with their subject matter? It sounds as though they are misinformed or simply unfamiliar with publications written on this subject. A good start would be to read articles written by Dr. Anthony S.-Y Leong on microwave fixation and successful IHC, and probably many others too. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From gcallis <@t> montana.edu Thu Jul 12 14:19:12 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Jul 12 14:18:59 2007 Subject: [Histonet] Xylene Substitutes??? In-Reply-To: <305373.14624.qm@web51808.mail.re2.yahoo.com> References: <305373.14624.qm@web51808.mail.re2.yahoo.com> Message-ID: <6.0.0.22.1.20070712131541.01b48b90@gemini.msu.montana.edu> We use them all the time for both processing and staining. Clearite 3 (Richard Allan) and have used and liked Propar (Anatech) too, both are single aliphatic hydrocarbons. We avoid the orange juice (citrus) smelling limonene type since they tend to make some of use ill. Some people have become sensitive to these. At 12:44 PM 7/12/2007, you wrote: >Has anyone had a good experience with any Xylene substitutes?? If so what >kind are you using?? > Thanks > > Michelle Seagle HT(ASCP) > > Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 From mpence <@t> grhs.net Thu Jul 12 14:34:42 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Jul 12 14:34:59 2007 Subject: [Histonet] Xylene Substitutes??? In-Reply-To: <305373.14624.qm@web51808.mail.re2.yahoo.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C66D@IS-E2K3.grhs.net> ProPar from Anatech http://anatechltdusa.com/ Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MICHELLE SEAGLE Sent: Thursday, July 12, 2007 1:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene Substitutes??? Has anyone had a good experience with any Xylene substitutes?? If so what kind are you using?? Thanks Michelle Seagle HT(ASCP) --------------------------------- Boardwalk for $500? In 2007? Ha! Play Monopoly Here and Now (it's updated for today's economy) at Yahoo! Games. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bwhitaker <@t> brownpathology.com Thu Jul 12 14:50:18 2007 From: bwhitaker <@t> brownpathology.com (Bonnie Whitaker) Date: Thu Jul 12 14:48:11 2007 Subject: [Histonet] Microwave processing In-Reply-To: <979FF5962E234F45B06CF0DB7C1AABB210BC807B@chi2k3ms01.columbuschildrens.net> Message-ID: <000a01c7c4bd$e0a4d3b0$3601a8c0@brownpathology.net> Hi All, I just wanted to throw my $0.02 in on this one. I suspect that what Ventana may have been referring to was the fixation used. I know that at least some of the microwave processing done out there is being done with "proprietary" reagents and not with formalin. Since virtually all of the literature (as well as the IVD kits) are based on formalin fixation for paraffin embedded tissue, then it is reasonable for an IHC company to say that you should use formalin fixation (especially in light of the CAP/oncologist fixation requirements for HER-2/neu). And, for the record, I'm just playing "devil's advocate" here, not defending Ventana in any form or fashion : ) Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Thursday, July 12, 2007 12:59 PM To: Rene J Buesa; Fischer, R. B; Bartlett, Jeanine; John PJ Coleman; histonet@lists.utsouthwestern.edu Cc: Keating, Jeffrey - MD; Delcambre,Linda V Subject: RE: [Histonet] Microwave processing I have never heard so much drivel; Ventana come out with some good ones every so often, but this takes the biscuit. Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Columbus Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 houstonr@chi.osu.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, July 12, 2007 1:49 PM To: Fischer, R. B; Bartlett, Jeanine; John PJ Coleman; histonet@lists.utsouthwestern.edu Cc: Keating, Jeffrey - MD; Delcambre,Linda V Subject: RE: [Histonet] Microwave processing Theoretically there should be NO difference in IHC reactions on tissue processed using microwave technology versus "conventional" tissue processing. You should have asked Ventana why do they "issued" that theoretically invalid warning, what tests have they done to support it. Ren? J. "Fischer, R. B" wrote: I have a question regarding IHC staining using tissues from a microwave processor. I recently attended an IHC workshop at Ventana. and was surprised to learn that IHC staining should not be carried out on any microwaved processed tissues. I have been pro microwave processing since they hit the marketplace, and am anxious to purchase one, but the info not to stain IHC from one has me worried. Can anyone share info in support of this statement? Thanks, R. Brian Fischer Histology Lead Tech Community Hospital of the Monterey Peninsula PO Box H.H. Monterey, Ca. 93942 831-625-4791 Fax: 831-625-4793 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Bartlett, Jeanine Sent: Wed 2/22/2006 3:12 PM To: John PJ Coleman; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Microwave processing We have the Sakura Xpress and we are still doing side-by-side comparisons on a variey of tissue types, IHC testing and special stains. So far the tissues form the Xpress have been as good as, and is some cases superior to, the traditional processing method. Jeanine Bartlett CDC Atlanta -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of John PJ Coleman Sent: Wed 2/22/2006 5:04 PM To: histonet@lists.utsouthwestern.edu Cc: Subject: [Histonet] Microwave processing I am the Senior tech of a large hospital corporation. My administration has just won funding for 4 Sakura Microwave rapid processing units. We run FISH her 2 on formalin fixed paraffin embedded tissue as per FDA protocol. As I tech, I am not in favor of tossing routine processing wholesale in favor of a completely new technology without thorough testing and parallel processing. Also, we are a regional reference lab for IHC and have a panel of 115 antibodies, all optimized for formalin fixed paraffin embedded tissue. We run an average of 350 IHC slides a day, max 580 per day and would have to re-optimize these to use in the new formalin free world while keeping our FFPE procedures in parallel for our reference lab work. Much like running 2 labs. If anyone has any insight, or if anyone currently uses these instruments for routine and/or IHC, feel free to call or email, and I'll check the postings on this string. We are also taking invitations to come out and see these things in use real time. John PJ Coleman-757 335-2159 http://members.cox.net/captainmadman/ http://journals.aol.com/jcolclefa/Waytoomuchboringcraptosayusually/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This is a transmission from Community Hospital of the Monterey Peninsula. This message and any attached documents may be confidential and contain information protected by state and federal medical privacy statutes. They are intended only for the use of the addressee. If you are not the intended recipient, any disclosure, copying, or distribution of this information is strictly prohibited. If you received this transmission in error, please accept our apologies and notify the sender. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Building a website is a piece of cake. Yahoo! Small Business gives you all the tools to get online. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: This e-mail message, from Children's Hospital, Columbus, Ohio, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes pursuant to Children's Hospital's confidentiality policies. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Thu Jul 12 14:55:20 2007 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Jul 13 20:44:01 2007 Subject: [Histonet] Xylene Substitutes??? In-Reply-To: <305373.14624.qm@web51808.mail.re2.yahoo.com> References: <305373.14624.qm@web51808.mail.re2.yahoo.com> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB7237@EMAIL.archildrens.org> We use XS-3 xylene substitute. We like it a lot. We get it from Statlab. It has very little odor and is a synthetic isoparaffinic hydrocarbon. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MICHELLE SEAGLE Sent: Thursday, July 12, 2007 1:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene Substitutes??? Has anyone had a good experience with any Xylene substitutes?? If so what kind are you using?? Thanks Michelle Seagle HT(ASCP) --------------------------------- Boardwalk for $500? In 2007? Ha! Play Monopoly Here and Now (it's updated for today's economy) at Yahoo! Games. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From jqb7 <@t> CDC.GOV Thu Jul 12 14:58:09 2007 From: jqb7 <@t> CDC.GOV (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Fri Jul 13 20:44:02 2007 Subject: [Histonet] Microwave processing References: <000a01c7c4bd$e0a4d3b0$3601a8c0@brownpathology.net> Message-ID: <34BB307EFC9A65429BBB49E330675F7201B0AADE@LTA3VS003.ees.hhs.gov> All of the microwave processors I am aware of can use formalin as well as any proprietary fixative they offer. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Bonnie Whitaker Sent: Thu 7/12/2007 3:50 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Microwave processing Hi All, I just wanted to throw my $0.02 in on this one. I suspect that what Ventana may have been referring to was the fixation used. I know that at least some of the microwave processing done out there is being done with "proprietary" reagents and not with formalin. Since virtually all of the literature (as well as the IVD kits) are based on formalin fixation for paraffin embedded tissue, then it is reasonable for an IHC company to say that you should use formalin fixation (especially in light of the CAP/oncologist fixation requirements for HER-2/neu). And, for the record, I'm just playing "devil's advocate" here, not defending Ventana in any form or fashion : ) Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Thursday, July 12, 2007 12:59 PM To: Rene J Buesa; Fischer, R. B; Bartlett, Jeanine; John PJ Coleman; histonet@lists.utsouthwestern.edu Cc: Keating, Jeffrey - MD; Delcambre,Linda V Subject: RE: [Histonet] Microwave processing I have never heard so much drivel; Ventana come out with some good ones every so often, but this takes the biscuit. Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Columbus Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 houstonr@chi.osu.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, July 12, 2007 1:49 PM To: Fischer, R. B; Bartlett, Jeanine; John PJ Coleman; histonet@lists.utsouthwestern.edu Cc: Keating, Jeffrey - MD; Delcambre,Linda V Subject: RE: [Histonet] Microwave processing Theoretically there should be NO difference in IHC reactions on tissue processed using microwave technology versus "conventional" tissue processing. You should have asked Ventana why do they "issued" that theoretically invalid warning, what tests have they done to support it. Ren? J. "Fischer, R. B" wrote: I have a question regarding IHC staining using tissues from a microwave processor. I recently attended an IHC workshop at Ventana. and was surprised to learn that IHC staining should not be carried out on any microwaved processed tissues. I have been pro microwave processing since they hit the marketplace, and am anxious to purchase one, but the info not to stain IHC from one has me worried. Can anyone share info in support of this statement? Thanks, R. Brian Fischer Histology Lead Tech Community Hospital of the Monterey Peninsula PO Box H.H. Monterey, Ca. 93942 831-625-4791 Fax: 831-625-4793 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Bartlett, Jeanine Sent: Wed 2/22/2006 3:12 PM To: John PJ Coleman; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Microwave processing We have the Sakura Xpress and we are still doing side-by-side comparisons on a variey of tissue types, IHC testing and special stains. So far the tissues form the Xpress have been as good as, and is some cases superior to, the traditional processing method. Jeanine Bartlett CDC Atlanta -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of John PJ Coleman Sent: Wed 2/22/2006 5:04 PM To: histonet@lists.utsouthwestern.edu Cc: Subject: [Histonet] Microwave processing I am the Senior tech of a large hospital corporation. My administration has just won funding for 4 Sakura Microwave rapid processing units. We run FISH her 2 on formalin fixed paraffin embedded tissue as per FDA protocol. As I tech, I am not in favor of tossing routine processing wholesale in favor of a completely new technology without thorough testing and parallel processing. Also, we are a regional reference lab for IHC and have a panel of 115 antibodies, all optimized for formalin fixed paraffin embedded tissue. We run an average of 350 IHC slides a day, max 580 per day and would have to re-optimize these to use in the new formalin free world while keeping our FFPE procedures in parallel for our reference lab work. Much like running 2 labs. If anyone has any insight, or if anyone currently uses these instruments for routine and/or IHC, feel free to call or email, and I'll check the postings on this string. We are also taking invitations to come out and see these things in use real time. John PJ Coleman-757 335-2159 http://members.cox.net/captainmadman/ http://journals.aol.com/jcolclefa/Waytoomuchboringcraptosayusually/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This is a transmission from Community Hospital of the Monterey Peninsula. This message and any attached documents may be confidential and contain information protected by state and federal medical privacy statutes. They are intended only for the use of the addressee. If you are not the intended recipient, any disclosure, copying, or distribution of this information is strictly prohibited. If you received this transmission in error, please accept our apologies and notify the sender. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Building a website is a piece of cake. Yahoo! Small Business gives you all the tools to get online. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: This e-mail message, from Children's Hospital, Columbus, Ohio, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes pursuant to Children's Hospital's confidentiality policies. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Thu Jul 12 15:08:03 2007 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Jul 13 20:44:03 2007 Subject: [Histonet] cap Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB7238@EMAIL.archildrens.org> For those of you who have experienced the unannounced cap inspections, how long after you received your checklists did your inspectors arrive? Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From TJJ <@t> Stowers-Institute.org Thu Jul 12 15:37:09 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Fri Jul 13 20:44:05 2007 Subject: [Histonet] Flex alcohols - diluting Message-ID: When I trained, I was taught to use 95% alcohol to make all my other alcohol dilutions because it was cheaper than using 100% alcohol (additionally, absolute ethanol had additional denaturants in it). With Richard Allan's Flex alcohols, the cost of the 95% alcohol is only about 3% less than the Flex100. So, is it really cheaper to use the 95% to make the dilutions than using Flex 100? So, if cost isn't the issue, is there any other reason not to buy just the 100% and make all the dilutions out from this one? I can't think of any. Thanks! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From jjohnson <@t> crispregional.org Thu Jul 12 15:43:33 2007 From: jjohnson <@t> crispregional.org (Jennifer Johnson) Date: Fri Jul 13 20:44:07 2007 Subject: [Histonet] (no subject) Message-ID: <000001c7c4c5$505a6010$2082010a@main.crispregional.org> Does anyone have a protocol for Surgipath's SelecTech Staining system? I appreciate it! From histotech <@t> charter.net Thu Jul 12 19:36:51 2007 From: histotech <@t> charter.net (histotech@charter.net) Date: Fri Jul 13 20:48:25 2007 Subject: [Histonet] Xylene Substitutes??? Message-ID: <305104139.1184287011560.JavaMail.root@fepweb10> - Formula 83 from CBG Biotech is excellent !!! DDietz --- Mike Pence wrote: > ProPar from Anatech http://anatechltdusa.com/ > Mike > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MICHELLE > SEAGLE > Sent: Thursday, July 12, 2007 1:44 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Xylene Substitutes??? > > > Has anyone had a good experience with any Xylene substitutes?? If so > what kind are you using?? > Thanks > > Michelle Seagle HT(ASCP) > > > --------------------------------- > Boardwalk for $500? In 2007? Ha! > Play Monopoly Here and Now (it's updated for today's economy) at Yahoo! > Games. _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mickie25 <@t> netzero.net Thu Jul 12 20:06:30 2007 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Fri Jul 13 20:48:26 2007 Subject: [Histonet] Clinical cryostat info needed In-Reply-To: <20070712120032635.00000005664@spikey> References: <20070712120032635.00000005664@spikey> Message-ID: Hi Leslie, The Leica 1850 UV has a UV light for decontamination. It is a great cryostat and easy to use. There are other brands out there some of which have decontamination capability. I'm sure Micron and Thermo/Shandon models do too. The Leica is the most ergonomic and the right height for the average person (5'2" to 5'10") with little stooping over. Use a 2.5 degree knife angle instead of 5 degrees it comes set at. Make sure the knife holder back plate screws and lower horizontal plate screws (also on the knife holder) are good and tight, when you first get it in. I have had chatter because of this a couple of times. Their anti-roll plate when adjusted correctly is the bomb. I have converted several avid 'I only use a brush' individuals. I set up Mohs labs and train Mohs technicians every week and they love Leica! Good Luck. Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lesley Bechtold Sent: Thursday, July 12, 2007 9:01 AM To: Histology Network Subject: [Histonet] Clinical cryostat info needed Hi, I need to provide information and approximate pricing for a clinical cryostat - one with a decontamination system. I would welcome responses from vendors but any info on the best model out there - particularly any models that are user-friendly for a wide range of potential users - would be welcome. Thank you Lesley Lesley S. Bechtold Senior Manager, Histopathology & Microscopy Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From garth <@t> apollosci.co.za Fri Jul 13 03:18:42 2007 From: garth <@t> apollosci.co.za (Garth Jerome) Date: Fri Jul 13 20:48:30 2007 Subject: [Histonet] Microwave processing In-Reply-To: <000a01c7c4bd$e0a4d3b0$3601a8c0@brownpathology.net> Message-ID: <001801c7c526$713694a0$7700a8c0@jhb.apollosci.co.za> Hello Milestone s.r.l produce a 'proprietary' fixative called FineFix. This is an ethanol based fixative. All clinical trials of this reagent have shown that it produces equal to or better IHC results than formalin fixed tissues. We use it occasionally for microwave enhanced fixation of fresh tissues and gross hardening of organs. It works beautifully! No IHC problems here. Regards Garth Jerome Apollo Scientific cc Telephone : 27-11-466 7666 Fascimile : 27-11-466 7672 Email : garth@apollosci.co.za -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonnie Whitaker Sent: 12 July 2007 09:50 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Microwave processing Hi All, I just wanted to throw my $0.02 in on this one. I suspect that what Ventana may have been referring to was the fixation used. I know that at least some of the microwave processing done out there is being done with "proprietary" reagents and not with formalin. Since virtually all of the literature (as well as the IVD kits) are based on formalin fixation for paraffin embedded tissue, then it is reasonable for an IHC company to say that you should use formalin fixation (especially in light of the CAP/oncologist fixation requirements for HER-2/neu). And, for the record, I'm just playing "devil's advocate" here, not defending Ventana in any form or fashion : ) Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Thursday, July 12, 2007 12:59 PM To: Rene J Buesa; Fischer, R. B; Bartlett, Jeanine; John PJ Coleman; histonet@lists.utsouthwestern.edu Cc: Keating, Jeffrey - MD; Delcambre,Linda V Subject: RE: [Histonet] Microwave processing I have never heard so much drivel; Ventana come out with some good ones every so often, but this takes the biscuit. Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Columbus Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 houstonr@chi.osu.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, July 12, 2007 1:49 PM To: Fischer, R. B; Bartlett, Jeanine; John PJ Coleman; histonet@lists.utsouthwestern.edu Cc: Keating, Jeffrey - MD; Delcambre,Linda V Subject: RE: [Histonet] Microwave processing Theoretically there should be NO difference in IHC reactions on tissue processed using microwave technology versus "conventional" tissue processing. You should have asked Ventana why do they "issued" that theoretically invalid warning, what tests have they done to support it. Ren? J. "Fischer, R. B" wrote: I have a question regarding IHC staining using tissues from a microwave processor. I recently attended an IHC workshop at Ventana. and was surprised to learn that IHC staining should not be carried out on any microwaved processed tissues. I have been pro microwave processing since they hit the marketplace, and am anxious to purchase one, but the info not to stain IHC from one has me worried. Can anyone share info in support of this statement? Thanks, R. Brian Fischer Histology Lead Tech Community Hospital of the Monterey Peninsula PO Box H.H. Monterey, Ca. 93942 831-625-4791 Fax: 831-625-4793 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Bartlett, Jeanine Sent: Wed 2/22/2006 3:12 PM To: John PJ Coleman; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Microwave processing We have the Sakura Xpress and we are still doing side-by-side comparisons on a variey of tissue types, IHC testing and special stains. So far the tissues form the Xpress have been as good as, and is some cases superior to, the traditional processing method. Jeanine Bartlett CDC Atlanta -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of John PJ Coleman Sent: Wed 2/22/2006 5:04 PM To: histonet@lists.utsouthwestern.edu Cc: Subject: [Histonet] Microwave processing I am the Senior tech of a large hospital corporation. My administration has just won funding for 4 Sakura Microwave rapid processing units. We run FISH her 2 on formalin fixed paraffin embedded tissue as per FDA protocol. As I tech, I am not in favor of tossing routine processing wholesale in favor of a completely new technology without thorough testing and parallel processing. Also, we are a regional reference lab for IHC and have a panel of 115 antibodies, all optimized for formalin fixed paraffin embedded tissue. We run an average of 350 IHC slides a day, max 580 per day and would have to re-optimize these to use in the new formalin free world while keeping our FFPE procedures in parallel for our reference lab work. Much like running 2 labs. If anyone has any insight, or if anyone currently uses these instruments for routine and/or IHC, feel free to call or email, and I'll check the postings on this string. We are also taking invitations to come out and see these things in use real time. John PJ Coleman-757 335-2159 http://members.cox.net/captainmadman/ http://journals.aol.com/jcolclefa/Waytoomuchboringcraptosayusually/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This is a transmission from Community Hospital of the Monterey Peninsula. This message and any attached documents may be confidential and contain information protected by state and federal medical privacy statutes. They are intended only for the use of the addressee. If you are not the intended recipient, any disclosure, copying, or distribution of this information is strictly prohibited. If you received this transmission in error, please accept our apologies and notify the sender. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Building a website is a piece of cake. Yahoo! Small Business gives you all the tools to get online. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: This e-mail message, from Children's Hospital, Columbus, Ohio, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes pursuant to Children's Hospital's confidentiality policies. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From louise.renton <@t> gmail.com Fri Jul 13 05:50:26 2007 From: louise.renton <@t> gmail.com (louise renton) Date: Fri Jul 13 20:48:33 2007 Subject: [Histonet] staining query Message-ID: Hi all, I have 2 questions to ask. The first is...If I have counterstained my immuno too heaviliy with haematoxylin, can I decolourise with acid alcohol & restain or will it affect the DAB? The second is.....Is there a tinctorial stain I can use to differentiate multinucleated giant cells from muscle? I cannot use immuno, as the tissue has been badly treated (poor things) and thus IHC is muddy & yucky. Thank you, best reagrds & have a great weekend ent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From doug <@t> ppspath.com Fri Jul 13 07:47:03 2007 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Fri Jul 13 20:48:34 2007 Subject: [Histonet] Microwave processing In-Reply-To: Message-ID: No offense Mr. Fisher but the information passed had to be misunderstood. Possibly what was meant was no IHC staining should be carried out on any microwaved processed tissues... until you internally validate it against conventional processing. Tissue must also be validated if you are using an alternative fixative on a conventional processor. We are using the Milestone Pathos microwave processor which still uses 10% NBF for fixation and we are not stuck paying for the outrageous costs of proprietary reagents. Testing has shown no difference in IHC results. If you are still skeptical about it I would make a call to Ventana and speak to them about the statement that was made in the workshop. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fischer, R. B Sent: Thursday, July 12, 2007 12:29 PM To: Bartlett, Jeanine; John PJ Coleman; histonet@lists.utsouthwestern.edu Cc: Keating, Jeffrey - MD; Delcambre,Linda V Subject: RE: [Histonet] Microwave processing I have a question regarding IHC staining using tissues from a microwave processor. I recently attended an IHC workshop at Ventana. and was surprised to learn that IHC staining should not be carried out on any microwaved processed tissues. I have been pro microwave processing since they hit the marketplace, and am anxious to purchase one, but the info not to stain IHC from one has me worried. Can anyone share info in support of this statement? Thanks, R. Brian Fischer Histology Lead Tech Community Hospital of the Monterey Peninsula PO Box H.H. Monterey, Ca. 93942 831-625-4791 Fax: 831-625-4793 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Bartlett, Jeanine Sent: Wed 2/22/2006 3:12 PM To: John PJ Coleman; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Microwave processing We have the Sakura Xpress and we are still doing side-by-side comparisons on a variey of tissue types, IHC testing and special stains. So far the tissues form the Xpress have been as good as, and is some cases superior to, the traditional processing method. Jeanine Bartlett CDC Atlanta -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of John PJ Coleman Sent: Wed 2/22/2006 5:04 PM To: histonet@lists.utsouthwestern.edu Cc: Subject: [Histonet] Microwave processing I am the Senior tech of a large hospital corporation. My administration has just won funding for 4 Sakura Microwave rapid processing units. We run FISH her 2 on formalin fixed paraffin embedded tissue as per FDA protocol. As I tech, I am not in favor of tossing routine processing wholesale in favor of a completely new technology without thorough testing and parallel processing. Also, we are a regional reference lab for IHC and have a panel of 115 antibodies, all optimized for formalin fixed paraffin embedded tissue. We run an average of 350 IHC slides a day, max 580 per day and would have to re-optimize these to use in the new formalin free world while keeping our FFPE procedures in parallel for our reference lab work. Much like running 2 labs. If anyone has any insight, or if anyone currently uses these instruments for routine and/or IHC, feel free to call or email, and I'll check the postings on this string. We are also taking invitations to come out and see these things in use real time. John PJ Coleman-757 335-2159 http://members.cox.net/captainmadman/ http://journals.aol.com/jcolclefa/Waytoomuchboringcraptosayusually/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This is a transmission from Community Hospital of the Monterey Peninsula. This message and any attached documents may be confidential and contain information protected by state and federal medical privacy statutes. They are intended only for the use of the addressee. If you are not the intended recipient, any disclosure, copying, or distribution of this information is strictly prohibited. If you received this transmission in error, please accept our apologies and notify the sender. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From igor.deyneko <@t> gmail.com Fri Jul 13 08:26:26 2007 From: igor.deyneko <@t> gmail.com (Igor Deyneko) Date: Fri Jul 13 20:56:10 2007 Subject: [Histonet] Methyl Green Counterstain Message-ID: <35e16a770707130626v4d0e9f86j4dd16ae8ac6e326c@mail.gmail.com> Hello. I am trying to do a Methyl Green counterstain on some cancer tissues, however the problem that I encounter is that the Methyl reen is quickly washed off the slides when done according to supllied protocol. Methyl Green is from DAKO. Protocol: For optimal results, use the following procedure: 1. Rehydrate tissue. 2. Stain tissue sections for 5 minutes with Methyl Green. 3. Rinse with 95% Ethanol to remove excess reagent. 4. Dehydrate through graded alcohol. 95% Ethanol 30 seconds with dipping 95% Ethanol 30 seconds with dipping 100% Ethanol 30 seconds with dipping 100% Ethanol 30 seconds with dipping Xylene or xylene substitute 2 minutes Xylene or xylene substitute 2 minutes 5. Mount in compatible permanent mounting media Can anyone offer an alternative processing , maybe with different times or alcohols? Thank you. Igor D. From TJJ <@t> Stowers-Institute.org Fri Jul 13 08:44:46 2007 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Fri Jul 13 20:57:48 2007 Subject: [Histonet] Flex alcohols - diluting Message-ID: > When I trained, I was taught to use 95% alcohol to make all my other > alcohol dilutions because it was cheaper than using 100% alcohol > (additionally, absolute ethanol had additional denaturants in it). > With Richard Allan's Flex alcohols, the cost of the 95% alcohol is > only about 3% less than the Flex100. So, is it really cheaper to use > the 95% to make the dilutions than using Flex 100? So, if cost isn't > the issue, is there any other reason not to buy just the 100% and make > all the dilutions out from this one? I can't think of any. > > Thanks! > > Teri Johnson, HT(ASCP)QIHC > Managing Director Histology Facility > Stowers Institute for Medical Research > 1000 E. 50th St. > Kansas City, MO 64110 > From HoustonR <@t> chi.osu.edu Fri Jul 13 08:56:57 2007 From: HoustonR <@t> chi.osu.edu (Houston, Ronald) Date: Fri Jul 13 20:58:38 2007 Subject: Recall: [Histonet] (no subject) Message-ID: <979FF5962E234F45B06CF0DB7C1AABB210BC833B@chi2k3ms01.columbuschildrens.net> Houston, Ronald would like to recall the message, "[Histonet] (no subject)". ----------------------------------------- Confidentiality Notice: This e-mail message, from Children's Hospital, Columbus, Ohio, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes pursuant to Children's Hospital's confidentiality policies. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From HoustonR <@t> chi.osu.edu Fri Jul 13 08:57:42 2007 From: HoustonR <@t> chi.osu.edu (Houston, Ronald) Date: Fri Jul 13 20:58:40 2007 Subject: [Histonet] (no subject) In-Reply-To: <4656575A.00018F.01757@bj126app3.126.com> Message-ID: <979FF5962E234F45B06CF0DB7C1AABB210BC833E@chi2k3ms01.columbuschildrens.net> Chunxia, The antibody has not been shown to work on formalin-fixed paraffin embedded tissue (check the spec sheet). My previous experience with dystroglycans and most other muscular dystrophy markers (Novocastra has the best antibodies for this type of work) is that they only work on frozen sections Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Columbus Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 houstonr@chi.osu.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ??? Sent: Thursday, May 24, 2007 11:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hello, everyone! I stained frozen and paraffin-embedded sections of human skeleton and kidney tissues using the Anti-?-Dystroglycan antibody, clone VIA4-1(catalog #: 05-298) from Upstate biotechnology company by immunohistochemistry. The method is: 3um sections, EDTA 8.0 heat retrieval(pressure cooking) 10min for paraffin section?antibody dilution is 1:50,1:100 and 1:200(25 degree, 2 hours), and addition of EnVision antibody complex, and then DAB. But there was no any positive signals. I do not know what is the matter. Please help me. Now, I need to know whether the dilution or reaction time is suitable. Please give me some good suggestions. I am eager to using the antibody smoothly. Thank you very much. Sincerely, Chunxia Zheng PhD Research Institute of Nephrology, Jinling Hospital 305 East Zhongshan Road Nanjing 210002, China Phone: 86-25-80860218 Fax: 86-25-84801992 ????????????? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: This e-mail message, from Children's Hospital, Columbus, Ohio, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes pursuant to Children's Hospital's confidentiality policies. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From jaina.negandhi <@t> sickkids.ca Fri Jul 13 09:32:22 2007 From: jaina.negandhi <@t> sickkids.ca (jaina.negandhi@sickkids.ca) Date: Fri Jul 13 21:02:48 2007 Subject: [Histonet] c-fos staining Message-ID: Hi, I am carrying out c-fos staining on free-floating cross-sections of the brain. I am trying to map out the entire brain, and need to use a marker to distinguish between the left and right sides, as they are symmetrical. Does anyone know of a marker (eg. dye) that I can use on a full brain sample without causing too much damage. I'd like to be able to mark it without gelatin embedding if possible. Thanks, Jaina Negandhi Research Project Coordinator Neuroscience and Mental Health Hospital for Sick Children Mc Master Building, RM 3006 Tel: 416-813-6551 Fax: 416-813-8724 From JMacDonald <@t> mtsac.edu Fri Jul 13 09:51:54 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Jul 13 21:04:28 2007 Subject: [Histonet] NSH House of Delegates Message-ID: The annual meeting of the House of Delegates of the National Society for Histotechnology will convene on October 31, 2007 at 7:00 PM in Denver, Colorado. The California Society for Histotechnology is looking for delegates to represent the state at this meeting. If you will be attending NSH this year and would like to represent our state please contact Jennifer MacDonald, State Secretary. You must be a current active member in good standing prior to the meeting. Thank you, Jennifer Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu From ryaskovich <@t> dir.nidcr.nih.gov Fri Jul 13 10:08:55 2007 From: ryaskovich <@t> dir.nidcr.nih.gov (Yaskovich, Ruth A (NIH/NIDCR) [E]) Date: Fri Jul 13 21:04:30 2007 Subject: [Histonet] Antigen Retrieval Solutions Message-ID: Does anyone reuse their Antigen Retrieval Solutions after heating? Ruth Yaskovich N.I.H. From msviapiano <@t> yahoo.com Fri Jul 13 12:21:16 2007 From: msviapiano <@t> yahoo.com (Mariano S. Viapiano) Date: Fri Jul 13 21:05:25 2007 Subject: [Histonet] Frozen samples in OCT. Message-ID: <36634.89462.qm@web54503.mail.re2.yahoo.com> Hi all, I just wanted to thank all the people that replied to my post about what to do with samples in OCT flash-frozen in liquid N2. We cut them as they came and had no problems staining them. Thanks! Mariano. Mariano S. Viapiano, PhD Assistant Professor Center for Molecular Neurobiology and Department of Neurological Surgery The Ohio State University 226B Rightmire Hall 1060 Carmack Rd., Columbus OH 43210 Tel (614) 292-4362 Fax (614) 292-5379 ____________________________________________________________________________________ Got a little couch potato? Check out fun summer activities for kids. http://search.yahoo.com/search?fr=oni_on_mail&p=summer+activities+for+kids&cs=bz From MadaryJ <@t> MedImmune.com Fri Jul 13 13:06:41 2007 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Fri Jul 13 21:05:27 2007 Subject: [Histonet] check the archive for xylene sub answers, etc Message-ID: <8F3E1865E343C943BB38506D56FF0151D37434@MD1EV002.medimmune.com> A query on xylene subs is welcome to the histonet since it brings out new answers, but you should also check the archives since it really give much more info since this is a really common question. Just a suggestion to supplement the responses. On a personal note I have had luck with many such as Formula 83, hemo de, d limonene, clear rite, for processing and many others that I cannot recall because of too much exposure to these items! I still like to use xylene for staining and coverslipping but most subs are fine for processing. I hear Richard Allan has great stuff too but I am not much for proprietary systems for many reasons. I know R.Allan has excellent systems that work. I do not use them, I recycle and make my own Hematoxylin and eosin and bluing agent to save money and have complete control over the H&E process. For processing I also use recycled xylene and alcohols except for the 100% which I still have to purchase of course. In seminars I have attended some of the newer techs out there using these proprietary systems have no idea what to do when things go wrong what to do, although the companies are very helpful in troubleshooting. Nick Madary, HT/HTL(ASCP)QIHC Lab Mgr 1, MedImmune, Inc. 301.398.6113/4745 """""""FAX 9745 From carl.hobbs <@t> kcl.ac.uk Fri Jul 13 13:41:32 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Fri Jul 13 21:05:29 2007 Subject: [Histonet] re:Transferring tissue from OCT to NBF Message-ID: <002b01c7c57d$6f63e4f0$4101a8c0@carlba65530bda> I personally prefer to allow the OCT-embedded frozen tissue to come to room temp( melt) before I rinse it in PBS and then place it in fixing fluid. ( eg: if it is a muscle bx, then I might need to re-orient before fixing....hence a reason for thawing before fixing) Depends on the tissue and it's pre-freezing orientation, imho. If it is ovary and it has been optimally frozen ( no ice-crystal artefact) then just do what you suggested. Caveat: One cannot guarantee that any results are of high fidelity unless you have an ovary that was fixed from fresh, as a positive control. Carl From Jessica.Vacca <@t> HCAhealthcare.com Fri Jul 13 14:34:01 2007 From: Jessica.Vacca <@t> HCAhealthcare.com (Vacca Jessica) Date: Fri Jul 13 21:05:31 2007 Subject: [Histonet] CAP unannounced visits Message-ID: <41E16A15CE78374EA45B57E0F94339B802912439@ORLEV01.hca.corpad.net> There was an article recently........can't remember when or where about what a lab did to be prepared for a CAP unannounced inspection. Can anyone tell me where I can find that? Thanks Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Drive Brandon, FL 33511 (813) 681-5551 ext 2454 (813) 571-5169 fax From Maxim_71 <@t> mail.ru Fri Jul 13 15:13:33 2007 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Fri Jul 13 21:05:32 2007 Subject: [Histonet] Xylene Substitutes??? Message-ID: <737742889.20070714001333@mail.ru> Earlier we worked with xylene, chloroform, mixes aliphatics hydrocarbons (C6-C10) for processing. Now we works with mineral oil. Our lab no have any processor. We will never come back to other clearites. Mineral oil is better than other in our opinion as for tissues as for personnel as for environment. Try this and it would like to you. Maxim Peshkov Russia, Taganrog. -----Original Message----- > Date: Thu, 12 Jul 2007 11:44:18 -0700 (PDT) > From: MICHELLE SEAGLE > Subject: [Histonet] Xylene Substitutes??? > To: histonet@lists.utsouthwestern.edu > Has anyone had a good experience with any Xylene substitutes?? If so what kind are you using?? > Thanks > > Michelle Seagle HT(ASCP) From Linke_Noelle <@t> Allergan.com Fri Jul 13 15:48:09 2007 From: Linke_Noelle <@t> Allergan.com (Linke_Noelle) Date: Fri Jul 13 21:05:36 2007 Subject: [Histonet] Davidson' schedule and GLP Message-ID: Hi everyone, I have a question for those of you who conduct GLP studies. When filling out forms, such as one which documents changing solutions (ex. Eyes from Davidson's to 70% etc), do you list all of the study sample numbers on the form or simply state the overall study number, assuming all specimens are included? Any help would be much appreciated. Thank you, No?lle Linke, MS, HTL(ASCP)QIHC Allergan, Inc 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5568 From JMacDonald <@t> mtsac.edu Fri Jul 13 17:36:58 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Jul 13 21:05:37 2007 Subject: [Histonet] NSH House of Delegates In-Reply-To: Message-ID: The annual meeting of the House of Delegates of the National Society for Histotechnology will convene on October 31, 2007 at 7:00 PM in Denver, Colorado. The California Society for Histotechnology is looking for delegates to represent the state at this meeting. If you will be attending NSH this year and would like to represent our state please contact Jennifer MacDonald, State Secretary. You must be a current active member in good standing prior to the meeting. Thank you, Jennifer Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu From jkiernan <@t> uwo.ca Fri Jul 13 22:13:23 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Jul 13 22:13:29 2007 Subject: [Histonet] staining query Message-ID: For your first question, Yes. The brown product of oxidation of DAB is not extracted or otherwise damaged by acidity and/or alcohol, so you can safely differentiate an overdone haemalum counterstain. For your second question, distinguishing multinucleate giant cells from muscle, how about lightly counterstaining with eosin Y after the haemalum? You'll then have an H&E preparation in which those cell types should look quite different. You didn't say what antigen you're immunostaining, or where it is. If it's in the cytoplasm of either muscle fibres or giant cells (osteoclasts? TB? foreign body?), the other other cell-type should be recognizable even if the eosin pink in immunopositive cells is obscured by the brown DAB oxidation product. A green anionic dye such as fast green FCF could be used instead of eosin, especially if the immunopositive material is in distinct granules, fibrils or other domains within the cytoplasm. Green with brown makes a more pleasing contrast than pink with brown. John Kiernan Anatomy, UWO London, Canada. -- ----- Original Message ----- From: louise renton Date: Friday, July 13, 2007 21:49 Subject: [Histonet] staining query To: Histonet@lists.utsouthwestern.edu > Hi all, I have 2 questions to ask. > > The first is...If I have counterstained my immuno too heaviliy with > haematoxylin, can I decolourise with acid alcohol & restain or > will it > affect the DAB? > > The second is.....Is there a tinctorial stain I can use to > differentiatemultinucleated giant cells from muscle? I cannot > use immuno, as the tissue > has been badly treated (poor things) and thus IHC is muddy & yucky. > > Thank you, best reagrds & have a great weekend _______________________________________________ From rjbuesa <@t> yahoo.com Sat Jul 14 07:32:22 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Jul 14 07:32:27 2007 Subject: [Histonet] Flex alcohols - diluting In-Reply-To: Message-ID: <926862.87519.qm@web61215.mail.yahoo.com> Using 95% EthOL to prepare lower EthOL concentrations is cumbersome for many. A 3% less cost does not in any way justify using 95% instead of 100% (absolute) EthOL and preparing the dilutions is straightforward assuring consistency in the lower dilutions. Ren? J. "Johnson, Teri" wrote: When I trained, I was taught to use 95% alcohol to make all my other alcohol dilutions because it was cheaper than using 100% alcohol (additionally, absolute ethanol had additional denaturants in it). With Richard Allan's Flex alcohols, the cost of the 95% alcohol is only about 3% less than the Flex100. So, is it really cheaper to use the 95% to make the dilutions than using Flex 100? So, if cost isn't the issue, is there any other reason not to buy just the 100% and make all the dilutions out from this one? I can't think of any. Thanks! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- It's here! Your new message! Get new email alerts with the free Yahoo! Toolbar. From rjbuesa <@t> yahoo.com Sat Jul 14 07:39:50 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Jul 14 07:39:56 2007 Subject: [Histonet] staining query In-Reply-To: Message-ID: <869758.20845.qm@web61211.mail.yahoo.com> Louise: You can differentiate (lower) the hematoxylin staining and it will not affect DAB staining BUT do it gently: prepare a 0.5% HCl in distilled water. Since hematoxylin acts as a pH indicator, immerse the slides one at a time in the solution until it turns PINK (in a few seconds); take the slide to a 1% aq. sol. of lithium carbonate until it turns BLUE; observe under the microscope to see if the contrat you want has been reached. If still too dark, repeat until you get the contrats you want. For the multinuclear cell use a Giemsa modification for tissue (under separate cover I am sending the protocol). Ren? J. louise renton wrote: Hi all, I have 2 questions to ask. The first is...If I have counterstained my immuno too heaviliy with haematoxylin, can I decolourise with acid alcohol & restain or will it affect the DAB? The second is.....Is there a tinctorial stain I can use to differentiate multinucleated giant cells from muscle? I cannot use immuno, as the tissue has been badly treated (poor things) and thus IHC is muddy & yucky. Thank you, best reagrds & have a great weekend ent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- It's here! Your new message! Get new email alerts with the free Yahoo! Toolbar. From rjbuesa <@t> yahoo.com Sat Jul 14 07:45:51 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Jul 14 07:45:55 2007 Subject: [Histonet] Methyl Green Counterstain In-Reply-To: <35e16a770707130626v4d0e9f86j4dd16ae8ac6e326c@mail.gmail.com> Message-ID: <452743.71407.qm@web61220.mail.yahoo.com> Your problem may reside in "Step 5" (COMPATIBLE permanent mounting medium). I can think of no other cause with the protocol you describe. Ren? J. Igor Deyneko wrote: Hello. I am trying to do a Methyl Green counterstain on some cancer tissues, however the problem that I encounter is that the Methyl reen is quickly washed off the slides when done according to supllied protocol. Methyl Green is from DAKO. Protocol: For optimal results, use the following procedure: 1. Rehydrate tissue. 2. Stain tissue sections for 5 minutes with Methyl Green. 3. Rinse with 95% Ethanol to remove excess reagent. 4. Dehydrate through graded alcohol. 95% Ethanol 30 seconds with dipping 95% Ethanol 30 seconds with dipping 100% Ethanol 30 seconds with dipping 100% Ethanol 30 seconds with dipping Xylene or xylene substitute 2 minutes Xylene or xylene substitute 2 minutes 5. Mount in compatible permanent mounting media Can anyone offer an alternative processing , maybe with different times or alcohols? Thank you. Igor D. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Pinpoint customers who are looking for what you sell. From rjbuesa <@t> yahoo.com Sat Jul 14 07:48:45 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Jul 14 07:48:52 2007 Subject: [Histonet] Antigen Retrieval Solutions In-Reply-To: Message-ID: <307415.97171.qm@web61224.mail.yahoo.com> You should NOT. The problem arises when using commercial (expensive) solutions. I used to make my own retrieval solutions and assured both low cost and the ability to NOT reusing them. Ren? J. "Yaskovich, Ruth A (NIH/NIDCR) [E]" wrote: Does anyone reuse their Antigen Retrieval Solutions after heating? Ruth Yaskovich N.I.H. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Fussy? Opinionated? Impossible to please? Perfect. Join Yahoo!'s user panel and lay it on us. From liz <@t> premierlab.com Sat Jul 14 10:51:31 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Sat Jul 14 10:51:42 2007 Subject: [Histonet] c-fos staining In-Reply-To: <27F06FF992C642569481718DF7BAAADD@PremierLab.local> References: <27F06FF992C642569481718DF7BAAADD@PremierLab.local> Message-ID: Jaina Why don't you make a notch or a small slice with a razor blade on one = side of the brain. Not too big that it would interfere with the = interpretation. We do this all the time with paraffin sections on rat = brain. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com =20 Ship to Address: =20 Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu = [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of = jaina.negandhi@sickkids.ca Sent: Friday, July 13, 2007 8:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] c-fos staining Hi, I am carrying out c-fos staining on free-floating cross-sections of the = brain. I am trying to map out the entire brain, and need to use a marker = to distinguish between the left and right sides, as they are = symmetrical. Does anyone know of a marker (eg. dye) that I can use on a full brain = sample without causing too much damage. I'd like to be able to mark it = without gelatin embedding if possible. Thanks, Jaina Negandhi Research Project Coordinator Neuroscience and Mental Health Hospital for Sick Children Mc Master Building, RM 3006 Tel: 416-813-6551 Fax: 416-813-8724 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG Free Edition.=20 Version: 7.5.476 / Virus Database: 269.10.5/899 - Release Date: = 7/13/2007 3:41 PM =20 No virus found in this outgoing message. Checked by AVG Free Edition.=20 Version: 7.5.476 / Virus Database: 269.10.5/899 - Release Date: = 7/13/2007 3:41 PM =20 From soper <@t> ciwemb.edu Sat Jul 14 11:55:57 2007 From: soper <@t> ciwemb.edu (Sarah Clatterbuck Soper) Date: Sat Jul 14 11:56:03 2007 Subject: [Histonet] Flex alcohols - diluting In-Reply-To: <926862.87519.qm@web61215.mail.yahoo.com> References: <926862.87519.qm@web61215.mail.yahoo.com> Message-ID: <4699001D.1080502@ciwemb.edu> Rene J Buesa wrote: > Using 95% EthOL to prepare lower EthOL concentrations is cumbersome > for many. A 3% less cost does not in any way justify using 95% > instead of 100% (absolute) EthOL and preparing the dilutions is > straightforward assuring consistency in the lower dilutions. Ren? J. When I was doing my lab rotations with an older faculty member he was showing me how to perform some protocol, and he asked suddenly, "So, how would you make 83% alcohol from 95%?" I hemmed and started looking for scratch paper. He shook his head sadly, muttering "what do they teach you all these days?" He explained that if you take a 100 mL graduated cylinder and pour 95% alcohol to 83 mL, then fill with water to 95 mL, you will have exactly 83% alcohol. This works for any percentage, and obviously the math is just as easy to make 950 mL as to make 95 mL. So I guess I disagree that making the dilutions is cumbersome. :-) Sarah From innvx <@t> sbcglobal.net Sat Jul 14 18:24:33 2007 From: innvx <@t> sbcglobal.net (INNOVEX BIOSCIENCES) Date: Sat Jul 14 18:24:40 2007 Subject: [Histonet] Animal IHC workshop, Friday August 17, 2007 Message-ID: <271819.64958.qm@web82004.mail.mud.yahoo.com> There are still a few spaces available for this hands-on wet workshop in San Francisco on August 17th, 2007. This is an oppurtunity for learning animal tissue IHC staining hands-on and it is not scheduled to be repeated in California. Reserve your space ASAP. Contact innvx@sbcglobal.net OR innvx.com From innvx <@t> sbcglobal.net Sat Jul 14 18:25:05 2007 From: innvx <@t> sbcglobal.net (INNOVEX BIOSCIENCES) Date: Sat Jul 14 18:25:10 2007 Subject: [Histonet] Animal IHC workshop, Friday August 17, 2007 Message-ID: <897948.18805.qm@web82007.mail.mud.yahoo.com> There are still a few spaces available for this hands-on wet workshop in San Francisco on August 17th, 2007. This is an oppurtunity for learning animal tissue IHC staining hands-on and it is not scheduled to be repeated in California. Reserve your space ASAP. Contact innvx@sbcglobal.net OR innvx.com From innvx <@t> sbcglobal.net Sat Jul 14 18:25:22 2007 From: innvx <@t> sbcglobal.net (INNOVEX BIOSCIENCES) Date: Sat Jul 14 18:25:25 2007 Subject: [Histonet] Animal IHC workshop, Friday August 17, 2007 Message-ID: <743432.72280.qm@web82004.mail.mud.yahoo.com> There are still a few spaces available for this hands-on wet workshop in San Francisco on August 17th, 2007. This is an opportunity for learning animal tissue IHC staining hands-on and it is not scheduled to be repeated in California. Reserve your space ASAP. Contact innvx@sbcglobal.net OR innvx.com From innvx <@t> sbcglobal.net Sat Jul 14 18:25:46 2007 From: innvx <@t> sbcglobal.net (INNOVEX BIOSCIENCES) Date: Sat Jul 14 18:25:49 2007 Subject: [Histonet] Animal IHC workshop, Friday August 17, 2007 Message-ID: <693048.80214.qm@web82002.mail.mud.yahoo.com> There are still a few spaces available for this hands-on wet workshop in San Francisco on August 17th, 2007. This is an opportunity for learning animal tissue IHC staining hands-on and it is not scheduled to be repeated in California. Reserve your space ASAP. Contact innvx@sbcglobal.net OR innvx.com From MVaughan4 <@t> ucok.edu Sat Jul 14 21:23:49 2007 From: MVaughan4 <@t> ucok.edu (MVaughan4@ucok.edu) Date: Sat Jul 14 21:24:14 2007 Subject: [Histonet] Methyl Green Counterstain at 60 degrees C Message-ID: I use methyl green from Vector Labs but the protocol says stain at 60 degrees C on a hot plate for 1 to 5 minutes. It looks OK and does not affect the immunostain. In fact it seems a bit weak. Mel Melville B. Vaughan, Ph.D. Assistant Professor of Biology University of Central Oklahoma Edmond, OK 73034 ----------------------------------------- **CONFIDENTIALITY** -This email (including any attachments) may contain confidential, proprietary and privileged information. Any unauthorized disclosure or use of this information is prohibited. From koellingr <@t> comcast.net Sat Jul 14 23:10:53 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Sat Jul 14 23:10:59 2007 Subject: [Histonet] Tracheal Whole Mounts Message-ID: <071520070410.12154.46999E4D0006BA6000002F7A22070216339D09020704040A0105@comcast.net> Kim, when I did this, I got them flat first (thin pins) and then immediately fixed them. Details too lengthy. But if you really want to find out about this from someone who is an expert, better than me, and that I worked with.... look up Thibaut De Smedt in PubMed or Google. He is easy to find articles written by him. He was a dendritic cell post-doc where I used to work and don't know where he is now but he is super nice and can give you all the info you want, far better than I on this very subject. Making the mounts and the IHC. His work was beautiful. Ray Koelling Phenopath Labs Seattle, WA -------------- Original message -------------- From: Kim Merriam > Hello everyone, > > I have been asked to do some IHC staining on mouse tracheal whole mounts. I was > wondering if anyone has done this; I am looking for information on how to > flatten out the trachea once it is removed from the animal. > > Do you think I should fix it first and then flatten it or fix and flatten at the > same time. Any thoughts or suggestions on how to flatten a mouse trachea would > be greatly appreciated. > > Thanks, > Kim > > Kim Merriam, MA, HT(ASCP) > Cambridge, MA > > > > ________________________________________________________________________________ > ____ > 8:00? 8:25? 8:40? Find a flick in no time > with the Yahoo! Search movie showtime shortcut. > http://tools.search.yahoo.com/shortcuts/#news > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Sat Jul 14 23:24:37 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Sat Jul 14 23:24:42 2007 Subject: [Histonet] Methyl Green Counterstain; also the name In-Reply-To: <35e16a770707130626v4d0e9f86j4dd16ae8ac6e326c@mail.gmail.com> References: <35e16a770707130626v4d0e9f86j4dd16ae8ac6e326c@mail.gmail.com> Message-ID: Alcohol-water mixtures rather easily remove cationic dyes from stained tissues, and "methyl" green is particularly easily removed. Water alone also removes this dye, especially if it is slightly acidic. Ordinary lab distilled or deionized water is commonly at pH 5 because of dissolved carbon dioxide. This is only slightly less acidic than the "methyl green" staining solution (usually pH 4). NB Kurnick (Stain Technol. 30:213-230, 1955) overcame the problem by transferring the stained slides, without washing, to filter paper, blotting, and then placing in two 5-minute changes of n-butanol. After the second n-butanol the slides can be cleared in xylene and covered in the usual way. A very brief rinse in water (5 sec with agitation) is permissible before blotting the stained slides. n-Butanol is only partly miscible with water and it has an unpleasant, irritating smell. Some people use acetone instead (see J Brachet Quart J. Microsc. Sci. 94: 1-10, 1953). I prefer n-butanol. We shouldn't really be calling the dye methyl green because that dye (CI 42585) has not been manufactured for perhaps 40 years. The dye sold under that name is really ethyl green (CI 42590), and it is greatly superior to the old methyl green. See Conn's Biological Stains, 9th or 10th ed, or The Sigma-Aldrich Handbook of Stains, Dyes and Indicators (FJ Green, 1990). Instead of mislabelling the bottles, vendors should be proudly selling this dye as ethyl green! John Kiernan Anatomy, UWO London, Canada --- ----- Original Message ----- From: Igor Deyneko Date: Friday, July 13, 2007 21:56 Subject: [Histonet] Methyl Green Counterstain To: histonet@lists.utsouthwestern.edu > Hello. > I am trying to do a Methyl Green counterstain on some cancer tissues, > however the problem that I encounter is that the Methyl reen is > quicklywashed off the slides when done according to supllied protocol. > Methyl Green is from DAKO. > Protocol: > > For optimal results, use the following procedure: > > 1. Rehydrate tissue. > > 2. Stain tissue sections for 5 minutes with Methyl Green. > > 3. Rinse with 95% Ethanol to remove excess reagent. > > 4. Dehydrate through graded alcohol. > > 95% Ethanol 30 seconds with dipping > > 95% Ethanol 30 seconds with dipping > > 100% Ethanol 30 seconds with dipping > > 100% Ethanol 30 seconds with dipping > > Xylene or xylene substitute 2 minutes > > Xylene or xylene substitute 2 minutes > > 5. Mount in compatible permanent mounting media > > > > Can anyone offer an alternative processing , maybe with > different times or > alcohols? > > Thank you. > > Igor D. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jkiernan <@t> uwo.ca Sat Jul 14 23:33:31 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Sat Jul 14 23:33:36 2007 Subject: [Histonet] Microwave processing Message-ID: Do the makers say what else is in their "ethanol based fixative", and do they cite peer-reviewed literature to support their "All clinical trials ..." claims? There is a large body of peer-reviewed scientific literature concerning fixatives that are mostly alcohol (methanol or ethanol) mixed with other liquids, notably water, acetic acid, chloroform and xylene. As coagulants of protein and DNA, these fixatives avert the antigen masking caused by aqueous formaldehyde. Alcohol alone is OK for fixing smaears or monolayer cell cultures, but it's no good for bits of tissue. Additives, notably acetic acid, allow ethanol and methanol to fix larger chunks of animal and plant organs. This stuff is all in the books. Any textbook published since about 1900 should be almost up to date on the physics and chemistry of fixation by non-aqueous liquids. Every histotechnical book published since 1950 contains many alcohol-acetic fixatives. It is argued that methanol is superior to ethanol, and the "methacarn" mixture (Carnoy's fluid with methanol replacing ethanol) has been thoroughly studied. It's important use it correctly, and not to pass the fixed tissue through any solvent mixture containing water. See H. Puchtler et al. (1970) Histochemie 21:97-116. Not following the corect procedure can make tissues unduly shrunken and tough to section. John Kiernan Anatomy, UWO London, Canada --- ----- Original Message ----- From: Garth Jerome Date: Friday, July 13, 2007 21:49 Subject: RE: [Histonet] Microwave processing To: histonet@lists.utsouthwestern.edu > Hello > Milestone s.r.l produce a 'proprietary' fixative called FineFix. > This is an > ethanol based fixative. All clinical trials of this reagent have > shown that > it produces equal to or better IHC results than formalin fixed > tissues. > We use it occasionally for microwave enhanced fixation of fresh > tissues and > gross hardening of organs. It works beautifully! > > No IHC problems here. > > Regards > > Garth Jerome > Apollo Scientific cc > Telephone : 27-11-466 7666 > Fascimile : 27-11-466 7672 > Email : garth@apollosci.co.za From jkiernan <@t> uwo.ca Sun Jul 15 01:44:18 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Sun Jul 15 01:44:23 2007 Subject: [Histonet] Tracheal Whole Mounts In-Reply-To: <071520070410.12154.46999E4D0006BA6000002F7A22070216339D09020704040A0105@comcast.net> References: <071520070410.12154.46999E4D0006BA6000002F7A22070216339D09020704040A0105@comcast.net> Message-ID: Dear Ray, Please tell us how you flattened the curved tracheal cartilages. To me, a tracheal whole-mount is just the posterior (= dorsal) wall of the trachea, which doesn't contain any cartilage. The tracheal cartilages of a rat cannot be made to lie flat, and for a mouse the exercise must be (forgive the next silly phrase), even more impossible. John Kiernan Anatomy, UWO London, Canada --- ----- Original Message ----- From: koellingr@comcast.net Date: Sunday, July 15, 2007 0:12 Subject: Re: [Histonet] Tracheal Whole Mounts To: Kim Merriam , Histonet > Kim, when I did this, I got them flat first (thin pins) and then > immediately fixed them. Details too lengthy. But if > you really want to find out about this from someone who is an > expert, better than me, and that I worked with.... look up > Thibaut De Smedt in PubMed or Google. He is easy to find > articles written by him. He was a dendritic cell post-doc > where I used to work and don't know where he is now but he is > super nice and can give you all the info you want, far better > than I on this very subject. Making the mounts and the > IHC. His work was beautiful. > > Ray Koelling > Phenopath Labs > Seattle, WA > > -------------- Original message -------------- > From: Kim Merriam > > > Hello everyone, > > > > I have been asked to do some IHC staining on mouse tracheal > whole mounts. I was > > wondering if anyone has done this; I am looking for > information on how to > > flatten out the trachea once it is removed from the animal. > > > > Do you think I should fix it first and then flatten it or fix > and flatten at the > > same time. Any thoughts or suggestions on how to flatten a > mouse trachea would > > be greatly appreciated. > > > > Thanks, > > Kim > > > > Kim Merriam, MA, HT(ASCP) > > Cambridge, MA > > > > > > > > > ________________________________________________________________________________ > > ____ > > 8:00? 8:25? 8:40? Find a flick in no time > > with the Yahoo! Search movie showtime shortcut. > > http://tools.search.yahoo.com/shortcuts/#news > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From webb3655 <@t> sbcglobal.net Sun Jul 15 09:04:24 2007 From: webb3655 <@t> sbcglobal.net (judy webb) Date: Sun Jul 15 09:04:31 2007 Subject: [Histonet] Job opening Message-ID: <494873.93807.qm@web83607.mail.sp1.yahoo.com> John Peter Smith Hospital, Fort Worth, Texas, has a opening for a Histotech. HT(ASCP) required, 3 years experience preferred. Contact: JPS Health Network, online application or call: Judy McKinney 817-927-1024 From mickie25 <@t> netzero.net Sun Jul 15 10:30:25 2007 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Sun Jul 15 10:31:31 2007 Subject: [Histonet] Job opening In-Reply-To: <494873.93807.qm@web83607.mail.sp1.yahoo.com> References: <494873.93807.qm@web83607.mail.sp1.yahoo.com> Message-ID: Hi All, Just a little more information about anti-roll plates on Leica cryostats. Regarding the anti-roll plate issue with having to adjust it all the time, one might try replacing the yoke portion (including the black adjustment knob). The new anti-roll plates have replaceable glass with 4 usable edges. If the knob has become worn (I experienced this where I trained in Charleston) the plate gets out of adjustment easily. The new yoke and glass should be adaptable to an existing swing arm. Belair Instruments sells both 'swings to the left' and swings toward the user' varieties (cost is about $175). An alternative is to replace the whole anti-roll plate apparatus with a new one (cost is about $250). The black knob on the new ones is stiffer and won't get out of adjustment as easily. If the glass edge is parallel to the knife, correct adjustment is a point just barely behind when the plate rubs on the face of the block so the rubbing stops (no noise on the up swing). If anyone would like more direct help, give me a call. Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net From pruegg <@t> ihctech.net Sun Jul 15 11:41:15 2007 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Sun Jul 15 11:38:52 2007 Subject: [Histonet] disaster plan Message-ID: <200707151638.l6FGcc4p070666@pro12.abac.com> Does anyone have a disaster plan they could share with me? I heard that their was one from CAP or Clia or someone out there but have not been able to find it. We want to have a protocol in place for what to do with precious tissue blocks stored from clinical trials in case of a disaster (flood, fire, earthquake, hurricane, etc.). is it recommended that these things be stored in fire safety cabinets? Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net From rjbuesa <@t> yahoo.com Sun Jul 15 13:21:21 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Jul 15 13:21:30 2007 Subject: [Histonet] disaster plan In-Reply-To: <200707151638.l6FGcc4p070666@pro12.abac.com> Message-ID: <968273.19026.qm@web61214.mail.yahoo.com> Our plan always called for blocks removal from premises. A fire safety cabinet (in case of a fire) will not prevent the blocks from melting. Ren? J. patsy ruegg wrote: Does anyone have a disaster plan they could share with me? I heard that their was one from CAP or Clia or someone out there but have not been able to find it. We want to have a protocol in place for what to do with precious tissue blocks stored from clinical trials in case of a disaster (flood, fire, earthquake, hurricane, etc.). is it recommended that these things be stored in fire safety cabinets? Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Pinpoint customers who are looking for what you sell. From marjoh3 <@t> telus.net Sun Jul 15 14:20:16 2007 From: marjoh3 <@t> telus.net (Marilyn Johnson) Date: Sun Jul 15 14:21:07 2007 Subject: [Histonet] Listeria by Immunohistochemistry Message-ID: <000a01c7c715$2d7bd7c0$6601a8c0@VALUED20606295> Hi Histonetters, I am looking for a primary antisera to stain for Listeria in bovine brain. The only source, now unavailable, was from Biodesign. Please let me know. Thanks in advance. Marilyn Johnson Alberta Agriculture Edmonton, AB. Canada From Rcartun <@t> harthosp.org Sun Jul 15 14:53:39 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Sun Jul 15 14:54:12 2007 Subject: [Histonet] Listeria by Immunohistochemistry In-Reply-To: <000a01c7c715$2d7bd7c0$6601a8c0@VALUED20606295> References: <000a01c7c715$2d7bd7c0$6601a8c0@VALUED20606295> Message-ID: <469A43030200007700006E5B@gwmail.harthosp.org> I use a polyclonal antibody that I obtained from DIFCO Laboratories (Detroit, MI) many years ago. I don't know if it's still available. I would be happy to stain some unstained slides for you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Marilyn Johnson" 07/15/07 3:20 PM >>> Hi Histonetters, I am looking for a primary antisera to stain for Listeria in bovine brain. The only source, now unavailable, was from Biodesign. Please let me know. Thanks in advance. Marilyn Johnson Alberta Agriculture Edmonton, AB. Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Mon Jul 16 00:02:19 2007 From: tkngflght <@t> yahoo.com (Cheryl R. Kerry) Date: Mon Jul 16 00:01:57 2007 Subject: [Histonet] Florida temp opening--seeking a licensed tech In-Reply-To: <494873.93807.qm@web83607.mail.sp1.yahoo.com> Message-ID: <000001c7c766$7d2ad860$0f061705@CHERYLSLAPTOP> Hi All- We're looking for a Florida licensed tech for a temp on the West Coast of the state. Premium pay, housing, per diem and all the other perks of travel work. Up to 13 weeks on day shift and in place as soon as possible... Give me a call at 800.756.3309 Ask for Cheryl. I've staffed this lab in the past--a good place to work. Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab, one great tech at a time. From koellingr <@t> comcast.net Mon Jul 16 00:09:46 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Mon Jul 16 00:09:53 2007 Subject: [Histonet] Tracheal Whole Mounts-long reply Message-ID: <071620070509.7217.469AFD9A000704F000001C3122070216339D09020704040A0105@comcast.net> A long reply at specifically targeted subject in case you are not interested you are notified. John/Kim/other interested parties, I'll give it a shot. With these 2 preamble pointers. 1) I've never done or even seen this with rat trachea so I'm at a loss how mouse and rat compare. 2) While I know a bit about immunology and IHC and consider myself a good anatomist with good dexterity, we had some people with incredible fine skills where I used to work that put me to shame. (Aside-we had someone who was working on ischemia projects and could tie off any part of a mouse aortic arch or put a ligation into the right common carotid, left common carotid or left subclavian, at will and at any part of the vessel, while the mouse was under anesthesia and do it perfectly mouse after mouse-and those are incredibly thin and tiny structures). Mouse whole mount tracheas: We used very, very small, sharp instruments. Very tiny, thin scissors. Very thin pins (I believe they are used by entomologists for delicate work). Etc. Open, with scissors, the trachea along the ventral mid-line and mount with the mucosa up. It is true you have (6 or 8????) curved tracheal rings to deal with. Again I've never done this with rat and I'm not sure if it is intuitive or counter-intuitive but those "springy curved rings" aren't too difficult to deal with in mouse. Maybe in rat they are thicker and springier and tighter to "flatten"???? Yet they do pose a slight problem. We used plates that were coated with Sylgard (Dow chemical silicone elastomer). Is 2 part liquid that sets up to hard silicon base after curing and perfect for holding pins tight and not wobbling. Can be poured to any thickness you want. I believe it comes in black also if you want that for color contrast in photos. So don't pin one edge with pin at 90 degrees. The tissue can just "spring" back up the pin from the hole in the tissue. Pin an edge, at a very acute angle (10 degrees), but do so by going across the tissue into the opposite edge and the silicone so that the pin is pinned but lying tight agross the tissue. Do so near a ring. At next ring, do same thing but in opposite direction. That is point the pin in opposite direction, go acroos tissue, pin into edge and into hard silicone so that that pin is lying tight down across the trachea. Don't need to do every ring but in end you have the trachea down flat (it is not mathematically and perfectly flat) with every other thin pin in opposite directions and each pin lying tight across the trachea but actually pinned down on a severe slant. Then fix in the plate / tissue according to needs. Formalin or in our case we used zinc/tris, fixed pins and all. We used zinc/tris as we were after dendritic cells/immune cells in the mucosa from mouse allergy models and could use all the nor mal flow antibodies and not worry about formalin sensitive epitopes and nasty retrievals. Another reason was that Thibaut that I mentioned was actually in a Belgian group that developed and marketed some sort of "Immunofix" and "immunowax" in Europe. Gayle Callis asked me about those products a while back. I told her and will still give odds that their immunofix was (is) zinc/tris that Beckstead/Nitta developed and BD sells. I got some of their "immunofix". Comapred to my homemade zinc/tris was same pH, same osmolarity, same severe temperature/pH gradient shift characteristic of Tris buffers and precipitated phosphate ions from PBS, presumably as zinc phosphate. I think Thibauts magic Immunofix is really zinc/tris but at 10,000x the true component cost. So back to the whole mounts, just left them pinned and worked on them like that, permealizing with Triton but I'm sure there are many other ways. After immunos would mount on a slide, permount , coverslip and a clamp to hol d coverslip flat till things dried. A second attack that I took was to cut 2 extremely thin pieces of silicon gasket. Then take out esophagous, cut long as before and using some very, very small alligator clips (electronic gear), clip the esophagous to a slide, mucosa up, a thin strip of silicone gasket at one extreme edge of "laid open esophagous" and clipped to the slide. Same thing on other side. So you had the esophagous flat, mucosa up but each edge under a strip og silicone gasket and from alligator clipped flat. Very edges useless but entire rest of structure exposed. Then fix and stain. In short, this was not something condusive to an SOP. Was part Rube Goldberg, part witchcraft, part luck, sweat, hopes and thinking. Certainly wasn't pretty looking but it did work. See Chiang Chieng-Hsun, Quantitative study of the ganglion neurons of mouse trachea, Cell and Tissue Research 246:2 Nov. 1986. Also James D Moffatt Role of epithelium and acetylcholine in mediating t he contraction of 5_HT in mouse isolated trachea. British Journal of Pharmacology (2004) 141 1159-1166. There are several others you can find who seem to do what we did - flat whole mount mouse trachea - although they did not describe the pinning down and some can't seem to spell SYLGARD from Dow which it looks like many people use to do this. Hope the pinning explaination was understandable. It is actually easier to do it than explaining how to do it. Ray Koelling Phenoptah Labs Seattle, WA -------------- Original message -------------- From: John Kiernan Dear Ray, Please tell us how you flattened the curved tracheal cartilages. To me, a tracheal whole-mount is just the posterior (= dorsal) wall of the trachea, which doesn't contain any cartilage. The tracheal cartilages of a rat cannot be made to lie flat, and for a mouse the exercise must be (forgive the next silly phrase), even more impossible. John Kiernan Anatomy, UWO London, Canada --- ----- Original Message ----- From: koellingr@comcast.net Date: Sunday, July 15, 2007 0:12 Subject: Re: [Histonet] Tracheal Whole Mounts To: Kim Merriam , Histonet > Kim, when I did this, I got them flat first (thin pins) and then > immediately fixed them. Details too lengthy. But if > you really want to find out about this from someone who is an > expert, better than me, and that I worked with.... look up > Thibaut De Smedt in PubMed or Google. He is easy to find > articles written by him. He was a dendritic cell post-doc > where I used to work and don't know where he is now but he is > super nice and can give you all the info you want, far better > than I on this very subject. Making the mounts and the > IHC. His work was beautiful. > > Ray Koelling > Phenopath Labs > Seattle, WA > > -------------- Original message -------------- > From: Kim Merriam > > > Hello everyone, > > > > I have been asked to do some IHC staining on mouse tracheal > whole mounts. I was > > wondering if anyone has done this; I am looking for > information on how to > > flatten out the trachea once it is removed from the animal. > > > > Do you think I should fix it first and then flatten it or fix > and flatten at the > > same time. Any thoughts or suggestions on how to flatten a > mouse trachea would > > be greatly appreciated. > > > > Thanks, > > Kim > > > > Kim Merriam, MA, HT(ASCP) > > Cambridge, MA > > > > > > > > > ________________________________________________________________________________ > > ____ > > 8:00? 8:25? 8:40? Find a flick in no time > > with the Yahoo! Search movie showtime shortcut. > > http://tools.search.yahoo.com/shortcuts/#news > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From louise.renton <@t> gmail.com Mon Jul 16 02:44:51 2007 From: louise.renton <@t> gmail.com (louise renton) Date: Mon Jul 16 02:44:55 2007 Subject: [Histonet] staining query II Message-ID: Thanks to all who replied to my query regarding decolourizing haem staining on immuo slides. I will follow up with Rene's r Giemsa procedure on the giant cell question. Thank you all so much!!! From settembr <@t> umdnj.edu Mon Jul 16 06:14:59 2007 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Mon Jul 16 06:15:35 2007 Subject: [Histonet] Oct-4, looking for it Message-ID: Hello, I am looking to find if any one is using or has used Oct-4 on formalin fixed paraffin embedded human tissue. None of the vendors I usually use seem to have it. What vendor? Dilution would be great too. Thank you for any help. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA From rcharles <@t> state.pa.us Mon Jul 16 07:05:29 2007 From: rcharles <@t> state.pa.us (Charles, Roger) Date: Mon Jul 16 07:06:10 2007 Subject: [Histonet] Listeria by Immunohistochemistry Message-ID: <12E4E17FEF6EBE4BAE95BEB3CDCB570006ACBC18@enhbgpri04.backup> In April of 2005 I ordered DIFCO's listeria O Poly Serotype 1&4 antibody from VWR using the catalog #90001-718 with a cost of $85.00 for 1ml. Works great on bovine brains and other species with no antigen retrieval needed. Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Sunday, July 15, 2007 3:54 PM To: histonet@lists.utsouthwestern.edu; Marilyn Johnson Subject: Re: [Histonet] Listeria by Immunohistochemistry I use a polyclonal antibody that I obtained from DIFCO Laboratories (Detroit, MI) many years ago. I don't know if it's still available. I would be happy to stain some unstained slides for you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Marilyn Johnson" 07/15/07 3:20 PM >>> Hi Histonetters, I am looking for a primary antisera to stain for Listeria in bovine brain. The only source, now unavailable, was from Biodesign. Please let me know. Thanks in advance. Marilyn Johnson Alberta Agriculture Edmonton, AB. Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Mon Jul 16 07:46:26 2007 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Mon Jul 16 07:46:32 2007 Subject: [Histonet] Oct-4, looking for it In-Reply-To: Message-ID: Hi Dana, We are using Santa Cruz's OCT 3/4 at 1:100 with Ventana's standard CC1 HIER solution on their instruments or 10" Biocare's BORG HIER solution in a pressure cooker, which is as harsh as we get for off-line retrieval. If you have any more questions let us know. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dana Settembre Sent: Monday, July 16, 2007 6:15 AM To: springrosycloud@126.com; Ronald Houston; histonet@lists.utsouthwestern.edu Cc: Catherine Susan Delia; Meera Hameed Subject: [Histonet] Oct-4, looking for it Hello, I am looking to find if any one is using or has used Oct-4 on formalin fixed paraffin embedded human tissue. None of the vendors I usually use seem to have it. What vendor? Dilution would be great too. Thank you for any help. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Mon Jul 16 08:39:40 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Mon Jul 16 08:39:48 2007 Subject: [Histonet] Oct-4, looking for it In-Reply-To: References: Message-ID: <469B3CDC0200007700006E8D@gwmail.harthosp.org> We have been using a goat pAb to Oct-3/4 (C-20) from Santa Cruz with good results. We use the antibody at a dilution of 1:100 (for 30 minutes) following heat retrieval. The only issue is that the antibody is labeled "RUO". Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Dana Settembre" 07/16/07 7:14 AM >>> Hello, I am looking to find if any one is using or has used Oct-4 on formalin fixed paraffin embedded human tissue. None of the vendors I usually use seem to have it. What vendor? Dilution would be great too. Thank you for any help. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Mon Jul 16 09:15:21 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Mon Jul 16 09:15:48 2007 Subject: [Histonet] c-fos staining In-Reply-To: References: Message-ID: <469B7D79.3090301@umdnj.edu> Hi Jaina: You can "paint" one side of the brain with: 1. a dilute aqueous solution (0.1% should be enough) of Alcian Blue OR 2. a dilute solution of aqueous picric acid. Geoff jaina.negandhi@sickkids.ca wrote: > Hi, > > I am carrying out c-fos staining on free-floating cross-sections of the > brain. I am trying to map out the entire brain, and need to use a marker to > distinguish between the left and right sides, as they are symmetrical. > > Does anyone know of a marker (eg. dye) that I can use on a full brain > sample without causing too much damage. I'd like to be able to mark it > without gelatin embedding if possible. > > Thanks, > > Jaina Negandhi > Research Project Coordinator > Neuroscience and Mental Health > Hospital for Sick Children > Mc Master Building, RM 3006 > Tel: 416-813-6551 > Fax: 416-813-8724 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From tim.morken <@t> thermofisher.com Mon Jul 16 11:08:03 2007 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Mon Jul 16 11:08:40 2007 Subject: [Histonet] Immunohistochemistry Quality Control Scientist position at ThermoFisher Scientific / Lab Vision, Fremont, CA, USA Message-ID: <6BFF6D137DF6BC43B33891BA96E83B19923902@PGHCR-EXMB-VS-1.na.fshrnet.com> Immunohistochemistry Quality Control Scientist Description The Analytical Technologies Group, Clinical Diagnostics Division, located in Fremont, CA has an immediate opening for an Immunohistochemistry (IHC) Quality Control Scientist whose primary responsibility will be to review and interpret IHC laboratory investigations and QC results. This position will review and approve IHC slides for product release and lot verification, provide guidance on troubleshooting of routine QC tests, assist the Product Surveillance (stability testing) program and assist in developing, designing, and performing experimental strategies and procedures for projects. In addition, you will support the transfer and release of new products and assist in developing various assays and transfers the technology to Manufacturing. Requirements Qualified candidates will have a BA/BS in biological sciences or related discipline plus a minimum of 5-6 years of pathology, histology and/or surgical pathology experience in a diagnostic, pharmaceutical, medical device or biotechnology environment. Extensive knowledge and skill in cell biology processes, histology, protein localization in tissue, pathology, and IHC reagent technology is required. Must have demonstrated success in technical proficiency, scientific creativity, and independent thought as well as proven skills in interpretation of scientific literature. The ability to analyze data and summarize results is essential. Strong troubleshooting, communication and organizational skills, as well as documentation of results and procedures. Strong word processing, spreadsheet, and imaging skills. Ability to use statistical techniques and experimental design. In-depth knowledge of IHC reagent technology and tissue staining techniques. Must have an understanding of protein, enzyme, and antibody functions and structures. ThermoFisher Scientific Thermo Fisher Scientific is the world leader in serving science. We provide a complete range of high-end analytical instruments, equipment, reagents and consumables, software and services for research, analysis, discovery and diagnostics. With annual sales of more than $9 billion, we employ 30,000 people in 150 countries worldwide, and serve over 350,000 customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions, government agencies, and environmental and industrial process control settings. All of our employees share a common set of values - Integrity, Intensity, Innovation and Involvement. Our ability to grow year after year is driven by our ability to attract, develop and retain world-class people who will thrive in our environment and share in our desire to improve mankind by enabling our customers to make the world healthier, cleaner and safer. If you share in our values and if you're looking for an employer who is strongly committed to developing talent and rewarding achievement, come grow with us at Thermo Fisher Scientific. Fremont, CA is in the East San Francisco Bay Area near San Jose. To apply online for this position go to www.thermofisher.com, click on "Careers" at the top right of the screen, click on {"Explore current opportunities available now", and enter the following code in the search box. Or search on California - Fremont, or Immunohistochemistry MA20072405-52862 Resumes can also be sent to me directly at tim.morken@thermofisher.com Tim Morken Technical Support Manager Anatomical Pathology ThermoFisher Scientific 47777 Warm Springs Blvd. Fremont, CA 94539 USA tel: 01-510-991-2840 fax: 01 510-991-2826 Tim.Morken@thermofisher.com web: www.labvision.com www.thermofisher.com The World Leader in Serving Science From apatel <@t> NYEE.EDU Mon Jul 16 11:21:58 2007 From: apatel <@t> NYEE.EDU (apatel@NYEE.EDU) Date: Mon Jul 16 11:20:34 2007 Subject: [Histonet] Connexin antibodies on Ventana Message-ID: <430728CDC2C8694886560ACC47D3B5F804AA0043@nyeeimail.nyee.edu> Anyone have experience using connexin antibodies on the Ventana Benchmark XT or equivalent with either frozen or formalin-fixed tissue? Any advice would be greatly appreciated. Anand D. Patel, MD PGY-4 Otolaryngology-Head & Neck Surgery New York Eye and Ear Infirmary (323) 839-8022 From paw555 <@t> yahoo.com Mon Jul 16 12:28:52 2007 From: paw555 <@t> yahoo.com (pam plumlee) Date: Mon Jul 16 12:28:57 2007 Subject: [Histonet] Open Positions/Pfizer, La Jolla, Ca Message-ID: <837036.54887.qm@web31912.mail.mud.yahoo.com> Hi All: Pfizer in La Jolla, Ca has 3 full-time histology technician positions open. In our state of the art laboratory we do necropsy, routine research histology and IHC (Dako and Ventana). Great learning opportunity for the right person! Jobs to be posted on the Pfizer website soon, until then please contact: Pamela Plumlee H.T. pam.plumlee@pfizer.com ____________________________________________________________________________________Ready for the edge of your seat? Check out tonight's top picks on Yahoo! TV. http://tv.yahoo.com/ From laurie.colbert <@t> huntingtonhospital.com Mon Jul 16 13:46:19 2007 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Mon Jul 16 13:46:32 2007 Subject: [Histonet] Earthquake Safety Message-ID: <57BE698966D5C54EAE8612E8941D768301268E33@EXCHANGE3.huntingtonhospital.com> I'm interested in knowing what other people in earthquake-prone areas do to secure their blocks and slides? Laurie Colbert Huntington Hospital Pasadena, CA From ploykasek <@t> phenopath.com Mon Jul 16 14:04:28 2007 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Mon Jul 16 14:04:38 2007 Subject: [Histonet] Blood group antigen Message-ID: HI all. I am desperately looking for an antibody to Blood Group H that will work on paraffin embedded tissue. We have been using 2 different antibodies in the last year, and both have been discontinued (1 discontinued after assurances that it would not be discontinued, but that's another story) I would appreciate any input. Thanks. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From mtarango <@t> nvcancer.org Mon Jul 16 15:14:46 2007 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Mon Jul 16 15:14:59 2007 Subject: [Histonet] Listeria by Immunohistochemistry In-Reply-To: <12E4E17FEF6EBE4BAE95BEB3CDCB570006ACBC18@enhbgpri04.backup> Message-ID: <5AEC610C1CE02945BD63A395BA763EDE011B6D30@NVCIEXCH02.NVCI.org> BIOCARE medical has one that works on FFPE tissue sections. Here is the link to the antibody datasheet. Note: OCT 4 is the same as OCT 3 and OCT 3/4. It's a pre-diluted antibody. http://www.biocare.net/antibodies/Oct_3_4.html Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles, Roger Sent: Monday, July 16, 2007 5:05 AM To: Richard Cartun; histonet@lists.utsouthwestern.edu; Marilyn Johnson Subject: RE: [Histonet] Listeria by Immunohistochemistry In April of 2005 I ordered DIFCO's listeria O Poly Serotype 1&4 antibody from VWR using the catalog #90001-718 with a cost of $85.00 for 1ml. Works great on bovine brains and other species with no antigen retrieval needed. Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Sunday, July 15, 2007 3:54 PM To: histonet@lists.utsouthwestern.edu; Marilyn Johnson Subject: Re: [Histonet] Listeria by Immunohistochemistry I use a polyclonal antibody that I obtained from DIFCO Laboratories (Detroit, MI) many years ago. I don't know if it's still available. I would be happy to stain some unstained slides for you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Marilyn Johnson" 07/15/07 3:20 PM >>> Hi Histonetters, I am looking for a primary antisera to stain for Listeria in bovine brain. The only source, now unavailable, was from Biodesign. Please let me know. Thanks in advance. Marilyn Johnson Alberta Agriculture Edmonton, AB. Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From gagnone <@t> KGH.KARI.NET Mon Jul 16 15:56:03 2007 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Mon Jul 16 15:56:09 2007 Subject: [Histonet] Red Box Slides..Residue Message-ID: We have been using the Red Box slides available from Newcomer Supply for our IHC controls. These slides have a painted red box, in our case on the back, non-frosted side of the slide. We cut the control section, pick up the section in the red box, then dry these slides and use as needed, picking up patient sections on the lower portion of the slide. Recently we have encountered some patchy staining and it was suggested by Ventana's customer service that the slides could be the cause. Their theory is that red paint from the back of a previous slide adheres to the front of the Red Box slide and repels reagents when they are dispensed onto the slide, causing incomplete staining on the patient section. Has anyone else had recent, similar experience with these slides doing the same thing? They are nice to use, so if there are others with the same problem, it would definitely affect our decision to discontinue their use and return to normal charged slides for our control cutting. Thanks for any assistance, Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada From JMacDonald <@t> mtsac.edu Mon Jul 16 16:33:42 2007 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon Jul 16 16:33:50 2007 Subject: [Histonet] Earthquake Safety In-Reply-To: <57BE698966D5C54EAE8612E8941D768301268E33@EXCHANGE3.huntingtonhospital.com> Message-ID: Laurie, At my previous place of employment we purchased locking cabinets for the block storage and the slide storage. We had converted to using cardboard file drawers for the blocks and slides for archive storage. Jennifer Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu "Laurie Colbert" Sent by: histonet-bounces@lists.utsouthwestern.edu 07/16/2007 11:46 AM To cc Subject [Histonet] Earthquake Safety I'm interested in knowing what other people in earthquake-prone areas do to secure their blocks and slides? Laurie Colbert Huntington Hospital Pasadena, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From igor.deyneko <@t> gmail.com Mon Jul 16 22:41:02 2007 From: igor.deyneko <@t> gmail.com (Igor Deyneko) Date: Mon Jul 16 22:41:07 2007 Subject: [Histonet] Methyl Gteen Counterstain Message-ID: <35e16a770707162041k39d84cf8tad0351c58f625acb@mail.gmail.com> Hello fellow colleagues! I am just starting out as a new histologist and I would like to thank everyone who responded.I recently have discovered this interesting and useful web site and I hope to receive in future your professional advices. Igor Deyneko. Infinity Pharmaceutical Cambridge, MA. From David.Lim <@t> vch.ca Mon Jul 16 23:08:28 2007 From: David.Lim <@t> vch.ca (Lim, David [NS]) Date: Mon Jul 16 23:08:45 2007 Subject: [Histonet] Red Box Slides..Residue Message-ID: We use control etched slides for our XT; so far, no apparent issues. The slides are manufactured by Erie and distributed through Fisher (#22-037-228). David Lim Anatomic Pathology Lions Gate Hospital Vancouver Coastal Health From jcarpenter764 <@t> aol.com Tue Jul 17 09:17:40 2007 From: jcarpenter764 <@t> aol.com (jcarpenter764@aol.com) Date: Tue Jul 17 09:17:49 2007 Subject: [Histonet] Any information on the Peloris processor Message-ID: <8C996945E9F43DD-1604-4518@mblk-d15.sysops.aol.com> Good morning Histoneers, I thought I would post an email to see if I could get any useful information from anyone in regards to the Peloris processor. Our lab is interested on this specific processor, but wanted to see if we could get any feedback, pros and/or cons regarding it. Thanks Jennell ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From tbailey <@t> uab.edu Tue Jul 17 09:34:54 2007 From: tbailey <@t> uab.edu (Tammy Bailey) Date: Tue Jul 17 09:35:10 2007 Subject: [Histonet] Good morning from Bama! Message-ID: <20070717143457.31782355502@mail.vsrc.uab.edu> Has anyone had good luck staining with 1% phenylenediamine? I will be staining optic nerve heads of tree shrews..Most of the papers I've found are from the stone age..Any help would be appreciated.. Thanks, Tammy M. Bailey University of Alabama-Birmingham Vision Science Research Center From c.weaver <@t> vla.defra.gsi.gov.uk Tue Jul 17 10:17:18 2007 From: c.weaver <@t> vla.defra.gsi.gov.uk (Weaver, Colin) Date: Tue Jul 17 10:17:35 2007 Subject: [Histonet] Microwave v conventional processing Message-ID: <7A885E8FE1C71C488D974EC601FAA690019E942B@vla-exchn1.cvlnt.vla.gov.uk> Hi - we are trying to go down the microwave route in processing but inevitably some of our veterinary pathologists are questioning whether microwave sections are as "good" as conventional processing. Can anyone point me in the right direction to find any comparison done between microwave processing and conventional overnight processing with regard to section and staining quality. Colin Weaver Veterinary Laboratories Agency (VLA) This email and any attachments is intended for the named recipient only. If you have received it in error you have no authority to use, disclose, store or copy any of its contents and you should destroy it and inform the sender. Whilst this email and associated attachments will have been checked for known viruses whilst within VLA systems we can accept no responsibility once it has left our systems. Communications on VLA's computer systems may be monitored and/or recorded to secure the effective operation of the system and for other lawful purposes. From rjbuesa <@t> yahoo.com Tue Jul 17 11:17:05 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jul 17 11:17:20 2007 Subject: [Histonet] Microwave v conventional processing In-Reply-To: <7A885E8FE1C71C488D974EC601FAA690019E942B@vla-exchn1.cvlnt.vla.gov.uk> Message-ID: <823923.82528.qm@web61220.mail.yahoo.com> Colin: Using the adequate protocols MW processing renders equivalent results to "conventional" tissue processing, that is the general concensus. The thing is that unless you use an automated MW tissue processor, a histotech will have to attend to the process and change reagents manually. This can lead to 2 problems: higher exposure of the HT to (usually hot) chemicals and some degree of inconsistency in the protocol because the time in each reagent could vary slightly different between runs. Consider that a few minutes in a conventional protocol is a much lower percentage of the time in the reagent, than the same amount of time in a much faster protocol completed with a MW tissue processor. MW processing should be an option when TAT is an issue and even then there are numerous manual steps independent of the time the tissue is involved in the processing step; they are independent of the processing technology and usually count for the greater part of the total TAT. Under separate cover I am sending you an article of mine wher I analyze this issue. Ren? J. "Weaver, Colin" wrote: Hi - we are trying to go down the microwave route in processing but inevitably some of our veterinary pathologists are questioning whether microwave sections are as "good" as conventional processing. Can anyone point me in the right direction to find any comparison done between microwave processing and conventional overnight processing with regard to section and staining quality. Colin Weaver Veterinary Laboratories Agency (VLA) This email and any attachments is intended for the named recipient only. If you have received it in error you have no authority to use, disclose, store or copy any of its contents and you should destroy it and inform the sender. Whilst this email and associated attachments will have been checked for known viruses whilst within VLA systems we can accept no responsibility once it has left our systems. Communications on VLA's computer systems may be monitored and/or recorded to secure the effective operation of the system and for other lawful purposes. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Fussy? Opinionated? Impossible to please? Perfect. Join Yahoo!'s user panel and lay it on us. From melissa.mazan <@t> tufts.edu Tue Jul 17 11:38:43 2007 From: melissa.mazan <@t> tufts.edu (Melissa Mazan) Date: Tue Jul 17 11:45:40 2007 Subject: [Histonet] immunostaining for sca-1 In-Reply-To: <200610171710.k9HHAqG2021407@mail-proofpoint-2a.usg.tufts.edu> References: <200610171710.k9HHAqG2021407@mail-proofpoint-2a.usg.tufts.edu> Message-ID: <469CF093.2020506@tufts.edu> Hi all, we're trying to stain simultaneously for sca-1 and SPC in mouse lung tissue - I'm unable to get the sca-1 to work (using a rat anti-mouse clone from BD) using immunofluorescence - so am trying the Vector ABC system. Unfortunately, to get the sca-1 to work, I have to leave out triton-X - without the Triton-X the SPC seems not to be working. Does anyone have advice for trying to simultaneously stain for an intracytoplasmic protein and a membrane protein at the same time? Is there a better fixative than 4% formaldehyde ( we leave the tissues in formaldehyde from 6hours to no more than 48 hours). Many thanks - Melissa Mazan Melissa R. Mazan, DVM, Diplomate ACVIM Associate Professor and Director of Equine Sports Medicine Department of Clinical Sciences Tufts Cumming School of Veterinary Medicine 200 Westborough Road North Grafton, MA 01536 Tel:508-839-5395 Fax:508-839-7922 email: melissa.mazan@tufts.edu histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Milestone Pathos vs Sakura Xpress (Gregor Arlt) > 2. RE: Milestone Pathos vs Sakura Xpress (Jes Strong) > 3. Re: Milestone Pathos vs Sakura Xpress (Rene J Buesa) > 4. RE: Milestone Pathos vs Sakura Xpress > (Bartlett, Jeanine (CDC/CCID/NCZVED)) > 5. seeking hiotology position in DC area (Steven Wilkes) > 6. Re: Vison BioSystems - Peloris (Anthony Reilly) > 7. Hello, fellow histotechies!!! This is my first time posing a > question - so be gentle. Our patholog (Yvonne Jones) > 8. RE: Milestone Pathos vs Sakura Xpress > (Bartlett, Jeanine (CDC/CCID/NCZVED)) > 9. RE: Hello, fellow histotechies!!! This is my first time > posing a question - so be gentle. Our patholog > (rdavis4@rdg.boehringer-ingelheim.com) > 10. RE: Hello, fellow histotechies!!! This is my first time > posing a question - so be gentle. Our patholog (soofia siddiqui) > 11. heat antigen retrieval methods (Peter Rippstein) > 12. problems with mouse brain fixation (Martina Urbanek) > 13. Phospho-S6 Ribosomal Protein (Goodwin, Diana) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 16 Oct 2006 14:53:33 -0500 > From: "Gregor Arlt" > Subject: [Histonet] Milestone Pathos vs Sakura Xpress > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; format=flowed > > Dear Histonetters, > > I have heard the Pathos would use 600 Watts for the microwave processing. I > can't imagine that this is right. In my opinion this would destroy the RNS > structure of any tissue. On the other hand I heard tissues processed in the > Xpress would be easy to use for molecularbiological investigations. > > Has anybody experience with those instruments, or know anybody the power of > the microwave of the instruments. > > Thanks for the help Frank > > _________________________________________________________________ > Get FREE company branded e-mail accounts and business Web site from > Microsoft Office Live > http://clk.atdmt.com/MRT/go/mcrssaub0050001411mrt/direct/01/ > > > > > ------------------------------ > > Message: 2 > Date: Mon, 16 Oct 2006 15:43:55 -0500 > From: "Jes Strong" > Subject: RE: [Histonet] Milestone Pathos vs Sakura Xpress > To: > Message-ID: <00a501c6f163$cc5b0010$0200a8c0@Jes> > Content-Type: text/plain; charset="us-ascii" > > Dear Mr. Arlt, > > If you would like to contact Milestone directly, we would be happy to > explain the principles of Milestone's microwave processors including > magnetron output and how it is dynamically regulated by software to adjust > to each specific load. These are not questions that users would, or should > be expected to be able to answer for you satisfactorily. > > Jes Strong > Western Region Sales Manager > Milestone Medical > (203) 925-4240 (Office) > (847) 323-8373 (Cell) > (847) 655-6009 (Fax) > jes@milestonemed.com > > www.milestonemed.com > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gregor Arlt > Sent: Monday, October 16, 2006 2:54 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Milestone Pathos vs Sakura Xpress > > Dear Histonetters, > > I have heard the Pathos would use 600 Watts for the microwave processing. I > can't imagine that this is right. In my opinion this would destroy the RNS > structure of any tissue. On the other hand I heard tissues processed in the > Xpress would be easy to use for molecularbiological investigations. > > Has anybody experience with those instruments, or know anybody the power of > the microwave of the instruments. > > Thanks for the help Frank > > _________________________________________________________________ > Get FREE company branded e-mail accounts and business Web site from > Microsoft Office Live > http://clk.atdmt.com/MRT/go/mcrssaub0050001411mrt/direct/01/ > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 3 > Date: Mon, 16 Oct 2006 14:19:18 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Milestone Pathos vs Sakura Xpress > To: Gregor Arlt , > histonet@lists.utsouthwestern.edu > Message-ID: <20061016211918.92177.qmail@web61214.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Frank: > The temperature produced by the magnetron is "controlled" by the temperature probe adjusted to the processing protocol; you can have 1200W and a working temperature of 50?C so wattage in itself is not a deletereous agent just provides the capability of getting to high temperatures quickly, if needed.. > Not all technologies are alike and the Xpress uses 60W continuously in the first 2 chambers. PATHOS is pure microwave technology and Xpress is a blend of MW and conventional technology. > In the Xpress the first 2 chambers are identical and using MW technology and bubble agitation. The other 2 are just 2 conventional retorts with convection heat and vacuum capabilities, operated at 65?C. > The molecular integrity does not relate to the process but to the tissue fixation. Formalin greatly prevents RNA studies but any alcoholic fixative like Kryofix, BoonFix or the propietary by Sakura (UMFix) will preserve the macromolecules either if the tissue is going to be processed with MW or conventional technology. Any good alcoholic fixative containin PEG also will preserve the macromolecules. > Both PATHOS and Xpress are "walk away" instruments but Xpress allows for the continuous addition of up to 30 cassettes every 15 minutes, for an overall work flow of 120 cassettes after 105 minutes, and 30 more every 15 minutes afterwards. The limit is the thickness of the sections (have to be 1.5mm thick) and some tissues have to be previously fixed from 4 to 4.5 hours before processing. > PATHOS can process tissues of up to 5mm at a rate of 210 cassettes/4 hours (fixation included) for thick tissue slices or 210 cassettes/1 hour for small biopsies. > Hope this information will help you! > Ren? J. > > Gregor Arlt wrote: > Dear Histonetters, > > I have heard the Pathos would use 600 Watts for the microwave processing. I > can't imagine that this is right. In my opinion this would destroy the RNS > structure of any tissue. On the other hand I heard tissues processed in the > Xpress would be easy to use for molecularbiological investigations. > > Has anybody experience with those instruments, or know anybody the power of > the microwave of the instruments. > > Thanks for the help Frank > > _________________________________________________________________ > Get FREE company branded e-mail accounts and business Web site from > Microsoft Office Live > http://clk.atdmt.com/MRT/go/mcrssaub0050001411mrt/direct/01/ > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > All-new Yahoo! Mail - Fire up a more powerful email and get things done faster. > > ------------------------------ > > Message: 4 > Date: Mon, 16 Oct 2006 18:46:57 -0400 > From: "Bartlett, Jeanine \(CDC/CCID/NCZVED\)" > Subject: RE: [Histonet] Milestone Pathos vs Sakura Xpress > To: "Gregor Arlt" , > > Message-ID: > > Content-Type: text/plain; charset="UTF-8" > > Frank, > > I have no experience with the Pathos but our lab does have the Sakura Xpress. I am attaching a PDF of the brochure for the equipment. Page 4 explains how the microwave itself operates (60 watts). One thing I want to add to the previous response is that the 1.5 mm thickness for the Xpress is not mandatory. There is allowance for thicker specimens but the processing time is then lengthened. But I for one like having an excuse to make pathologists gross properly to begin with. :) > > Jeanine Bartlett > CDC, Atlanta > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Gregor Arlt > Sent: Mon 10/16/2006 3:53 PM > To: histonet@lists.utsouthwestern.edu > Cc: > Subject: [Histonet] Milestone Pathos vs Sakura Xpress > > > > Dear Histonetters, > > I have heard the Pathos would use 600 Watts for the microwave processing. I > can't imagine that this is right. In my opinion this would destroy the RNS > structure of any tissue. On the other hand I heard tissues processed in the > Xpress would be easy to use for molecularbiological investigations. > > Has anybody experience with those instruments, or know anybody the power of > the microwave of the instruments. > > Thanks for the help Frank > > _________________________________________________________________ > Get FREE company branded e-mail accounts and business Web site from > Microsoft Office Live > http://clk.atdmt.com/MRT/go/mcrssaub0050001411mrt/direct/01/ > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 5 > Date: Mon, 16 Oct 2006 22:15:25 -0400 > From: "Steven Wilkes" > Subject: [Histonet] seeking hiotology position in DC area > To: histonet@lists.utsouthwestern.edu > Message-ID: > <8e5827cf0610161915w1f450489mb6aec23f432807ae@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Hello > > I am seeking a histology position in the greater DC area. I have a MA in > biology, 2+ years of histology and immunohistochemistry experience, as well > as a bit histology teaching. Thank you > > Steven > > > ------------------------------ > > Message: 6 > Date: Tue, 17 Oct 2006 12:37:52 +1000 > From: "Anthony Reilly" > Subject: Re: [Histonet] Vison BioSystems - Peloris > To: , > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > Hi Richard > > My peloris was part of the very first relase of the instrument. As a > result there were some initial minor problems which have since been > rectified. As I said these problems were minor and never in any way > affected the processing of our tissue. > > It now runs very well and has had a positive impact on our laboratory. > The instrument has a very powerful mixing ability which not only > improves penetration but guarantees even heating on the steps where heat > is utilised giving shorter processing times even for fatty tissue. This > is aided by using one of their range of specimen baskets which separates > each cassette individually allowing better flow of solution to each > specimen. > > Our laboratory services both heart and lung transplant units requiring > us to run numeroous short cycles throughout the day. The improved > processing combined with a rapid clean cycle means that the one dual > retort peloris can do the work of 3-4 of our prevoius instruments. > > Examples of our improved times include: > > Fatty tissue 18h to 14h > Routine 12h to 9h > Small Biopsy 2h to 1 h > > This has also had an impact on IHC as the small biopsies that are > required urgently can be given an extra 1h in formalin and still be > completed in the same time as the previous protocol. With the faster > processing some tissues such as lletz biopsies need to be processed on > shorter cycles to avoid hardening of the tissue. According to the > manufacturer these times can be reduced further by substituting xylene > with isopropanol but I have not tried that so cannot comment. > > regards > > > > Tony Reilly > Chief Scientist > Anatomical Pathology > QHPS-Prince Charles Hospital > Rode Rd Chermside Q 4032 > Australia > Ph: 07 3139 4543 > Fax: 07 3193 4546 > tony_reilly@health.qld.gov.au > > > >>>> "Richard Cartun" 10/15/06 11:46 pm >>> >>>> > Anyone out there using Vison BioSystems' "Peloris" for tissue > processing? If so, what has been your experience? Thank you. > > Richard > > Richard W. Cartun, Ph.D. > Director, Immunopathology & Histology > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 > (860) 545-0174 Fax > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ***************************************************************** > This email, including any attachments sent with it, is > confidential and for the sole use of the intended recipient(s). > This confidentiality is not waived or lost, if you receive it and > you are not the intended recipient(s), or if it is transmitted/ > received in error. > > Any unauthorised use, alteration, disclosure, distribution or > review of this email is strictly prohibited. The information > contained in this email, including any attachment sent with > it, may be subject to a statutory duty of confidentiality if it > relates to health service matters. > > If you are not the intended recipient(s), or if you have > received this email in error, you are asked to immediately > notify the sender by telephone collect on Australia > +61 1800 198 175 or by return email. You should also > delete this email, and any copies, from your computer > system network and destroy any hard copies produced. > > If not an intended recipient of this email, you must not copy, > distribute or take any action(s) that relies on it; any form of > disclosure, modification, distribution and/or publication of this > email is also prohibited. > > Although Queensland Health takes all reasonable steps to > ensure this email does not contain malicious software, > Queensland Health does not accept responsibility for the > consequences if any person's computer inadvertently suffers > any disruption to services, loss of information, harm or is > infected with a virus, other malicious computer programme or > code that may occur as a consequence of receiving this > email. > > Unless stated otherwise, this email represents only the views > of the sender and not the views of the Queensland Government. > **************************************************************** > > > > > ------------------------------ > > Message: 7 > Date: Tue, 17 Oct 2006 02:43:21 -0400 > From: "Yvonne Jones" > Subject: [Histonet] Hello, fellow histotechies!!! This is my first > time posing a question - so be gentle. Our patholog > To: > Message-ID: <453443490200004F00000225@GWGATE1.ahm.com> > Content-Type: text/plain; charset="US-ASCII" > > Hello, fellow histotechies!!! This is my first time posing a question - > so be gentle. Our pathologists have recently requested that we begin > testing an antibody, p52. I have had a bear of a time finding any > information about this antibody!!! All I could find was one article > on-line, and I am still having a problem finding info and the antibody > itself. > > ----------------------------------------- > > > > > > ------------------------------ > > Message: 8 > Date: Tue, 17 Oct 2006 05:49:06 -0400 > From: "Bartlett, Jeanine \(CDC/CCID/NCZVED\)" > Subject: RE: [Histonet] Milestone Pathos vs Sakura Xpress > To: "Bartlett, Jeanine \(CDC/CCID/NCZVED\)" , "Gregor > Arlt" , > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Apparently the PDF was too large for Histonet to accept. You can go to > the Sakura website and click on Xpress and open the brochure if you need > these details. > > Jeanine > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Bartlett, Jeanine (CDC/CCID/NCZVED) > Sent: Monday, October 16, 2006 6:47 PM > To: Gregor Arlt; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Milestone Pathos vs Sakura Xpress > > Frank, > > I have no experience with the Pathos but our lab does have the Sakura > Xpress. I am attaching a PDF of the brochure for the equipment. Page 4 > explains how the microwave itself operates (60 watts). One thing I want > to add to the previous response is that the 1.5 mm thickness for the > Xpress is not mandatory. There is allowance for thicker specimens but > the processing time is then lengthened. But I for one like having an > excuse to make pathologists gross properly to begin with. :) > > Jeanine Bartlett > CDC, Atlanta > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > Gregor Arlt > Sent: Mon 10/16/2006 3:53 PM > To: histonet@lists.utsouthwestern.edu > Cc: > Subject: [Histonet] Milestone Pathos vs Sakura Xpress > > > > Dear Histonetters, > > I have heard the Pathos would use 600 Watts for the microwave > processing. I > can't imagine that this is right. In my opinion this would > destroy the RNS > structure of any tissue. On the other hand I heard tissues > processed in the > Xpress would be easy to use for molecularbiological > investigations. > > Has anybody experience with those instruments, or know anybody > the power of > the microwave of the instruments. > > Thanks for the help Frank > > > _________________________________________________________________ > Get FREE company branded e-mail accounts and business Web site > from > Microsoft Office Live > http://clk.atdmt.com/MRT/go/mcrssaub0050001411mrt/direct/01/ > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > ------------------------------ > > Message: 9 > Date: Tue, 17 Oct 2006 07:46:17 -0400 > From: rdavis4@rdg.boehringer-ingelheim.com > Subject: RE: [Histonet] Hello, fellow histotechies!!! This is my first > time posing a question - so be gentle. Our patholog > To: yjones2@csmlab.com, histonet@lists.utsouthwestern.edu > Message-ID: > <83BA2D3D42947D48BDAA449453644ABE0865B1@RDGEXM01.am.boehringer.com> > Content-Type: text/plain; charset=us-ascii > > Yvonne, > > Google p52 and lots of hits come up. Also, check out www.abcam.com. They > have p52 as a rabbit polyclonal. > > Rebecca A. Davis, A.A.S., NYS LVT, HT (ASCP) > Toxicology, Histopathology Lab > Boehringer-Ingelheim Pharmaceuticals, Inc. > rdavis4@rdg.boehringer-ingelheim.com > 203-798-5448 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yvonne Jones > Sent: Tuesday, October 17, 2006 2:43 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Hello, fellow histotechies!!! This is my first time > posing a question - so be gentle. Our patholog > > Hello, fellow histotechies!!! This is my first time posing a question - > so be gentle. Our pathologists have recently requested that we begin > testing an antibody, p52. I have had a bear of a time finding any > information about this antibody!!! All I could find was one article > on-line, and I am still having a problem finding info and the antibody > itself. > > ----------------------------------------- > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 10 > Date: Tue, 17 Oct 2006 07:39:31 -0700 (PDT) > From: soofia siddiqui > Subject: RE: [Histonet] Hello, fellow histotechies!!! This is my first > time posing a question - so be gentle. Our patholog > To: rdavis4@rdg.boehringer-ingelheim.com, yjones2@csmlab.com, > histonet@lists.utsouthwestern.edu > Message-ID: <20061017143931.16702.qmail@web39513.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Go to Google and go check for Biocompare.com. Biocompare is a very good source to search for any bio products. I have searched and found all of the antibodies, that Dako has discontinued, through this web site. Good luck! > Soofia > > rdavis4@rdg.boehringer-ingelheim.com wrote: > Yvonne, > > Google p52 and lots of hits come up. Also, check out www.abcam.com. They > have p52 as a rabbit polyclonal. > > Rebecca A. Davis, A.A.S., NYS LVT, HT (ASCP) > Toxicology, Histopathology Lab > Boehringer-Ingelheim Pharmaceuticals, Inc. > rdavis4@rdg.boehringer-ingelheim.com > 203-798-5448 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yvonne Jones > Sent: Tuesday, October 17, 2006 2:43 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Hello, fellow histotechies!!! This is my first time > posing a question - so be gentle. Our patholog > > Hello, fellow histotechies!!! This is my first time posing a question - > so be gentle. Our pathologists have recently requested that we begin > testing an antibody, p52. I have had a bear of a time finding any > information about this antibody!!! All I could find was one article > on-line, and I am still having a problem finding info and the antibody > itself. > > ----------------------------------------- > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Get your email and more, right on the new Yahoo.com > > ------------------------------ > > Message: 11 > Date: Tue, 17 Oct 2006 11:36:56 -0400 > From: "Peter Rippstein" > Subject: [Histonet] heat antigen retrieval methods > To: > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > Hello Histonetters, > > Our lab is in need of some information in regards heat antigen > retrieval methods. Has anyone done a comparison in terms of results & > cost effectiveness obtained from microwave irradiation vs pressure > cooking and steam heating methods. Any recommendations would be > appreciated. Many thanks. > Peter > > > Peter Rippstein ART, MLT > Core Pathology Laboratory > Rm H2102 > University of Ottawa Heart Institute > 40 Ruskin Street > Ottawa, Ontario > Canada, K1Y 4W7 > > Tel: (613) 761-5282 > Fax: (613) 761-5281 > Email: prippstein@ottawaheart.ca > > > > > > > -------------- next part -------------- > BEGIN:VCARD > VERSION:2.1 > X-GWTYPE:USER > FN:Peter Rippstein > EMAIL;WORK;PREF;NGW:PRippstein@ottawaheart.ca > N:Rippstein;Peter > END:VCARD > > BEGIN:VCARD > VERSION:2.1 > X-GWTYPE:USER > FN:Peter Rippstein > EMAIL;WORK;PREF;NGW:PRippstein@ottawaheart.ca > N:Rippstein;Peter > END:VCARD > > BEGIN:VCARD > VERSION:2.1 > X-GWTYPE:USER > FN:Peter Rippstein > EMAIL;WORK;PREF;NGW:PRippstein@ottawaheart.ca > N:Rippstein;Peter > END:VCARD > > BEGIN:VCARD > VERSION:2.1 > X-GWTYPE:USER > FN:Peter Rippstein > EMAIL;WORK;PREF;NGW:PRippstein@ottawaheart.ca > N:Rippstein;Peter > END:VCARD > > > ------------------------------ > > Message: 12 > Date: Tue, 17 Oct 2006 17:51:41 +0200 > From: Martina Urbanek > Subject: [Histonet] problems with mouse brain fixation > To: histonet@lists.utsouthwestern.edu > Message-ID: <1161100301.4534fc0d23f8a@web-mail1.uibk.ac.at> > Content-Type: text/plain; charset=ISO-8859-1 > > Hello everybody on histonet, > > we have some problems with handling mouse (21 and 90 days old) and rat brains. > We do mouse brain perfusion using 4% paraformaldehyde in PBS, pH 7.4. We use > gravity for perfusion for about 30 minutes (volume about 80 ml) and after > perfusion we leave the brains over night in the same fixative. Then we process > in a Shandon tissue processor (70% Alcohol, 80% Alcohol, 95% Alcohol, 3 changes > 100% alcohol, 3 changes xylene, 2 changes paraffin 56?C; time depends on size > of tissue). Now we have the problem that some brains are too hard and some also > seem to shrink more than others, when we cut them and put them on waterbath > they seem to expand and distort and often brittle. So that it looks like only > fibrous tissue is left, the structure is gone. I already had a look on histonet > archive but did not find anything that helps, therefore I hope that someone has > an idea what can be wrong. I have to say that we don?t have any problems when > we immersion fix the brains with 4% formaldehyde. > The problems we have with rat brains are a bit different, because we get the > brains from another group who perfuse the lung with 4% paraformaldehyde in > Hepes-buffer, pH 7.35. We then postfix over night in the same fixative they > use (when the brain is also perfused) or for 3 days (when brain is not > perfused). After dehydration in tissue processor we have the same cutting > problems like we observe with mouse brain tissue, even worse. > Any suggestions are appreciated! > > Thank you very much for your help!!! > > Martina Urbanek > > > > Ms. Martina Urbanek > Forschungslabor der > Klin.Abt. f?r Neonatologie > neonatal neuroscience research laboratory > Med. University Innsbruck > Innrain 66, 4th floor > A-6020 Innsbruck > Tel. +43 (0)512 504 27755/27765 > Fax: +43 (0)512 504 27766 > Email: Martina.Urbanek@uibk.ac.at > > > > > > > > > > > > ------------------------------ > > Message: 13 > Date: Tue, 17 Oct 2006 12:52:24 -0400 > From: "Goodwin, Diana" > Subject: [Histonet] Phospho-S6 Ribosomal Protein > To: , > > Message-ID: > <80CDD9C3FEEAFD4982B114C4A6DFD00E02CB8235@uphsmbx2.UPHS.PENNHEALTH.PRV> > > Content-Type: text/plain; charset="us-ascii" > > For those of us 'Netters using this Ab on human tissue, which one and at > what dilution? > > Thanks! > > Diana Goodwin > Supervisor, Anatomic Pathology > Pennsylvania Hospital > Preston 655-C > ph. 215-829-6532 > pager 215-422-5160 > fax 215-829-7564 > e-mail goodwind@[pahosp.com > > > > The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 35, Issue 28 > **************************************** > From mcauliff <@t> umdnj.edu Tue Jul 17 12:08:09 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Jul 17 12:15:10 2007 Subject: [Histonet] Good morning from Bama! In-Reply-To: <20070717143457.31782355502@mail.vsrc.uab.edu> References: <20070717143457.31782355502@mail.vsrc.uab.edu> Message-ID: <469CF779.1090401@umdnj.edu> Hi Tammy:/ p/-phenlyenediamine is a reducing agent used to reduce osmium and darken the osmophilic tissue elements. I don't think it will stain anything by itself. By the way, it is a serious error to dismiss research simply because it is "old". How do you think we got to current scientific knowledge, by reinventing the wheel every time technology changes? Of course, if you prefer to ignore the "stone age" and make the same mistakes over and over again ..................... Geoff Tammy Bailey wrote: > Has anyone had good luck staining with 1% phenylenediamine? I will be > staining optic nerve heads of tree shrews..Most of the papers I've found are > from the stone age..Any help would be appreciated.. > > > > Thanks, > > Tammy M. Bailey > > University of Alabama-Birmingham > > Vision Science Research Center > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From bsylinda <@t> aol.com Tue Jul 17 12:15:47 2007 From: bsylinda <@t> aol.com (bsylinda@aol.com) Date: Tue Jul 17 12:19:48 2007 Subject: [Histonet] CYTOKERATIN QUESTION Message-ID: <8C996AD409DCB12-17EC-5B6F@mblk-d47.sysops.aol.com> Hello Histoland, Can anyone in histoland inform me of the best cytokeratin antibody to use to differenate squamous cell carcinoma and adenocarcinoma.? Any feedback would be greatly appreciated. Thanks in advance, Sylinda ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From jessgrocki <@t> yahoo.com Tue Jul 17 12:20:39 2007 From: jessgrocki <@t> yahoo.com (Jessica Piche) Date: Tue Jul 17 12:20:44 2007 Subject: [Histonet] Cytology Cell Block Retention Message-ID: <428287.2672.qm@web82002.mail.mud.yahoo.com> Hi Everyone, I was wondering if anyone can tell me what the CAP requirement is for Cell blocks? How long to we need to keep them for? Are they treated like a regular pathology paraffin block? Thanks so much!! Jessica Piche-Grocki, HT(ASCP) From mpence <@t> grhs.net Tue Jul 17 12:32:14 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Jul 17 12:32:25 2007 Subject: [Histonet] Cytology Cell Block Retention In-Reply-To: <428287.2672.qm@web82002.mail.mud.yahoo.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C672@IS-E2K3.grhs.net> 10 years -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica Piche Sent: Tuesday, July 17, 2007 12:21 PM To: histonet Subject: [Histonet] Cytology Cell Block Retention Hi Everyone, I was wondering if anyone can tell me what the CAP requirement is for Cell blocks? How long to we need to keep them for? Are they treated like a regular pathology paraffin block? Thanks so much!! Jessica Piche-Grocki, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From asmith <@t> mail.barry.edu Tue Jul 17 12:34:35 2007 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Tue Jul 17 12:34:43 2007 Subject: [Histonet] RE:papers from the stone age Message-ID: <7DBCCC1FBC77C94F99F920D0CA6400B61E40C0@exchsrv01.barrynet.barry.edu> I am very grateful to journals like Ca: A Cancer Journal for Clinicians for occasionally reprinting classic papers, notably Sir James Paget's original description of Paget's disease from 1874. Comparative anatomy would be next to impossible without interlibrary loans of ancient classics: Garman's 1889 paper is the only description of the odd lateral line organ of the bonnet shark - I was lucky to ger a photocopy from the Bryn Mawr library. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From JPCOLEMA <@t> sentara.com Tue Jul 17 14:00:48 2007 From: JPCOLEMA <@t> sentara.com (JOHN P COLEMAN) Date: Tue Jul 17 14:01:24 2007 Subject: [Histonet] Microwave processors In-Reply-To: <20070717165633.0DB39A831D@spamnet01.sentara.com> References: <20070717165633.0DB39A831D@spamnet01.sentara.com> Message-ID: I did an extensi ve validation on the Sakura Xpress. 60 watts by the way, and what i found is that if grossing is done properly, tissue processes well no matter the system. I have less reprocessing on Xpress than on VIP even when pathologists gross too thick. PA's who have been through a BS or MS program all gross appropriately. Nobody here grosses at 1.5, and we use both the 67 and the 120 min programs depending mainly on tissue type. Short for bxs where no gross cutting happens and for less dense tissue and the long for most surgicals. We have a 7 hospital system, 4 xpress processors. John P.J. Coleman HT(ASCP)QIHC Clinical Specialist Anatomic Pathology Sentara Laboratory Services Cell/Voicemail(757)335-2159 pager: (757)456-6695 >>> 7/17/2007 12:56 PM >>> Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Open Positions/Pfizer, La Jolla, Ca (pam plumlee) 2. Earthquake Safety (Laurie Colbert) 3. Blood group antigen (Patti Loykasek) 4. RE: Listeria by Immunohistochemistry (Tarango, Mark) 5. Red Box Slides..Residue (Gagnon, Eric) 6. Re: Earthquake Safety (Jennifer MacDonald) 7. Methyl Gteen Counterstain (Igor Deyneko) 8. Re: Red Box Slides..Residue (Lim, David [NS]) 9. Any information on the Peloris processor (jcarpenter764@aol.com) 10. Good morning from Bama! (Tammy Bailey) 11. Microwave v conventional processing (Weaver, Colin) 12. Re: Microwave v conventional processing (Rene J Buesa) 13. immunostaining for sca-1 (Melissa Mazan) ---------------------------------------------------------------------- Message: 1 Date: Mon, 16 Jul 2007 10:28:52 -0700 (PDT) From: pam plumlee Subject: [Histonet] Open Positions/Pfizer, La Jolla, Ca To: histonet@lists.utsouthwestern.edu Message-ID: <837036.54887.qm@web31912.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi All: Pfizer in La Jolla, Ca has 3 full-time histology technician positions open. In our state of the art laboratory we do necropsy, routine research histology and IHC (Dako and Ventana). Great learning opportunity for the right person! Jobs to be posted on the Pfizer website soon, until then please contact: Pamela Plumlee H.T. pam.plumlee@pfizer.com ____________________________________________________________________________________Ready for the edge of your seat? Check out tonight's top picks on Yahoo! TV. http://tv.yahoo.com/ ------------------------------ Message: 2 Date: Mon, 16 Jul 2007 11:46:19 -0700 From: "Laurie Colbert" Subject: [Histonet] Earthquake Safety To: Message-ID: <57BE698966D5C54EAE8612E8941D768301268E33@EXCHANGE3.huntingtonhospital.com> Content-Type: text/plain; charset="iso-8859-1" I'm interested in knowing what other people in earthquake-prone areas do to secure their blocks and slides? Laurie Colbert Huntington Hospital Pasadena, CA ------------------------------ Message: 3 Date: Mon, 16 Jul 2007 12:04:28 -0700 From: Patti Loykasek Subject: [Histonet] Blood group antigen To: histonet Message-ID: Content-Type: text/plain; charset="US-ASCII" HI all. I am desperately looking for an antibody to Blood Group H that will work on paraffin embedded tissue. We have been using 2 different antibodies in the last year, and both have been discontinued (1 discontinued after assurances that it would not be discontinued, but that's another story) I would appreciate any input. Thanks. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. ------------------------------ Message: 4 Date: Mon, 16 Jul 2007 13:14:46 -0700 From: "Tarango, Mark" Subject: RE: [Histonet] Listeria by Immunohistochemistry To: "Charles, Roger" , "Richard Cartun" , histonet@lists.utsouthwestern.edu, "Marilyn Johnson" Message-ID: <5AEC610C1CE02945BD63A395BA763EDE011B6D30@NVCIEXCH02.NVCI.org> Content-Type: text/plain; charset=us-ascii BIOCARE medical has one that works on FFPE tissue sections. Here is the link to the antibody datasheet. Note: OCT 4 is the same as OCT 3 and OCT 3/4. It's a pre-diluted antibody. http://www.biocare.net/antibodies/Oct_3_4.html Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles, Roger Sent: Monday, July 16, 2007 5:05 AM To: Richard Cartun; histonet@lists.utsouthwestern.edu; Marilyn Johnson Subject: RE: [Histonet] Listeria by Immunohistochemistry In April of 2005 I ordered DIFCO's listeria O Poly Serotype 1&4 antibody from VWR using the catalog #90001-718 with a cost of $85.00 for 1ml. Works great on bovine brains and other species with no antigen retrieval needed. Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Sunday, July 15, 2007 3:54 PM To: histonet@lists.utsouthwestern.edu; Marilyn Johnson Subject: Re: [Histonet] Listeria by Immunohistochemistry I use a polyclonal antibody that I obtained from DIFCO Laboratories (Detroit, MI) many years ago. I don't know if it's still available. I would be happy to stain some unstained slides for you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Marilyn Johnson" 07/15/07 3:20 PM >>> Hi Histonetters, I am looking for a primary antisera to stain for Listeria in bovine brain. The only source, now unavailable, was from Biodesign. Please let me know. Thanks in advance. Marilyn Johnson Alberta Agriculture Edmonton, AB. Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== ------------------------------ Message: 5 Date: Mon, 16 Jul 2007 16:56:03 -0400 From: "Gagnon, Eric" Subject: [Histonet] Red Box Slides..Residue To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" We have been using the Red Box slides available from Newcomer Supply for our IHC controls. These slides have a painted red box, in our case on the back, non-frosted side of the slide. We cut the control section, pick up the section in the red box, then dry these slides and use as needed, picking up patient sections on the lower portion of the slide. Recently we have encountered some patchy staining and it was suggested by Ventana's customer service that the slides could be the cause. Their theory is that red paint from the back of a previous slide adheres to the front of the Red Box slide and repels reagents when they are dispensed onto the slide, causing incomplete staining on the patient section. Has anyone else had recent, similar experience with these slides doing the same thing? They are nice to use, so if there are others with the same problem, it would definitely affect our decision to discontinue their use and return to normal charged slides for our control cutting. Thanks for any assistance, Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada ------------------------------ Message: 6 Date: Mon, 16 Jul 2007 14:33:42 -0700 From: Jennifer MacDonald Subject: Re: [Histonet] Earthquake Safety To: "Laurie Colbert" Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Laurie, At my previous place of employment we purchased locking cabinets for the block storage and the slide storage. We had converted to using cardboard file drawers for the blocks and slides for archive storage. Jennifer Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu "Laurie Colbert" Sent by: histonet-bounces@lists.utsouthwestern.edu 07/16/2007 11:46 AM To cc Subject [Histonet] Earthquake Safety I'm interested in knowing what other people in earthquake-prone areas do to secure their blocks and slides? Laurie Colbert Huntington Hospital Pasadena, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Mon, 16 Jul 2007 22:41:02 -0500 From: "Igor Deyneko" Subject: [Histonet] Methyl Gteen Counterstain To: histonet@lists.utsouthwestern.edu Message-ID: <35e16a770707162041k39d84cf8tad0351c58f625acb@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hello fellow colleagues! I am just starting out as a new histologist and I would like to thank everyone who responded.I recently have discovered this interesting and useful web site and I hope to receive in future your professional advices. Igor Deyneko. Infinity Pharmaceutical Cambridge, MA. ------------------------------ Message: 8 Date: Mon, 16 Jul 2007 21:08:28 -0700 From: "Lim, David [NS]" Subject: Re: [Histonet] Red Box Slides..Residue To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" We use control etched slides for our XT; so far, no apparent issues. The slides are manufactured by Erie and distributed through Fisher (#22-037-228). David Lim Anatomic Pathology Lions Gate Hospital Vancouver Coastal Health ------------------------------ Message: 9 Date: Tue, 17 Jul 2007 10:17:40 -0400 From: jcarpenter764@aol.com Subject: [Histonet] Any information on the Peloris processor To: histonet@lists.utsouthwestern.edu Message-ID: <8C996945E9F43DD-1604-4518@mblk-d15.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Good morning Histoneers, I thought I would post an email to see if I could get any useful information from anyone in regards to the Peloris processor. Our lab is interested on this specific processor, but wanted to see if we could get any feedback, pros and/or cons regarding it. Thanks Jennell ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. ------------------------------ Message: 10 Date: Tue, 17 Jul 2007 09:34:54 -0500 From: "Tammy Bailey" Subject: [Histonet] Good morning from Bama! To: Message-ID: <20070717143457.31782355502@mail.vsrc.uab.edu> Content-Type: text/plain; charset="us-ascii" Has anyone had good luck staining with 1% phenylenediamine? I will be staining optic nerve heads of tree shrews..Most of the papers I've found are from the stone age..Any help would be appreciated.. Thanks, Tammy M. Bailey University of Alabama-Birmingham Vision Science Research Center ------------------------------ Message: 11 Date: Tue, 17 Jul 2007 16:17:18 +0100 From: "Weaver, Colin" Subject: [Histonet] Microwave v conventional processing To: Message-ID: <7A885E8FE1C71C488D974EC601FAA690019E942B@vla-exchn1.cvlnt.vla.gov.uk> Content-Type: text/plain; charset="us-ascii" Hi - we are trying to go down the microwave route in processing but inevitably some of our veterinary pathologists are questioning whether microwave sections are as "good" as conventional processing. Can anyone point me in the right direction to find any comparison done between microwave processing and conventional overnight processing with regard to section and staining quality. Colin Weaver Veterinary Laboratories Agency (VLA) This email and any attachments is intended for the named recipient only. If you have received it in error you have no authority to use, disclose, store or copy any of its contents and you should destroy it and inform the sender. Whilst this email and associated attachments will have been checked for known viruses whilst within VLA systems we can accept no responsibility once it has left our systems. Communications on VLA's computer systems may be monitored and/or recorded to secure the effective operation of the system and for other lawful purposes. ------------------------------ Message: 12 Date: Tue, 17 Jul 2007 09:17:05 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Microwave v conventional processing To: "Weaver, Colin" , histonet@lists.utsouthwestern.edu Message-ID: <823923.82528.qm@web61220.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Colin: Using the adequate protocols MW processing renders equivalent results to "conventional" tissue processing, that is the general concensus. The thing is that unless you use an automated MW tissue processor, a histotech will have to attend to the process and change reagents manually. This can lead to 2 problems: higher exposure of the HT to (usually hot) chemicals and some degree of inconsistency in the protocol because the time in each reagent could vary slightly different between runs. Consider that a few minutes in a conventional protocol is a much lower percentage of the time in the reagent, than the same amount of time in a much faster protocol completed with a MW tissue processor. MW processing should be an option when TAT is an issue and even then there are numerous manual steps independent of the time the tissue is involved in the processing step; they are independent of the processing technology and usually count for the greater part of the total TAT. Under separate cover I am sending you an article of mine wher I analyze this issue. Ren? J. "Weaver, Colin" wrote: Hi - we are trying to go down the microwave route in processing but inevitably some of our veterinary pathologists are questioning whether microwave sections are as "good" as conventional processing. Can anyone point me in the right direction to find any comparison done between microwave processing and conventional overnight processing with regard to section and staining quality. Colin Weaver Veterinary Laboratories Agency (VLA) This email and any attachments is intended for the named recipient only. If you have received it in error you have no authority to use, disclose, store or copy any of its contents and you should destroy it and inform the sender. Whilst this email and associated attachments will have been checked for known viruses whilst within VLA systems we can accept no responsibility once it has left our systems. Communications on VLA's computer systems may be monitored and/or recorded to secure the effective operation of the system and for other lawful purposes. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Fussy? Opinionated? Impossible to please? Perfect. Join Yahoo!'s user panel and lay it on us. ------------------------------ Message: 13 Date: Tue, 17 Jul 2007 12:38:43 -0400 From: Melissa Mazan Subject: [Histonet] immunostaining for sca-1 To: histonet@lists.utsouthwestern.edu Message-ID: <469CF093.2020506@tufts.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hi all, we're trying to stain simultaneously for sca-1 and SPC in mouse lung tissue - I'm unable to get the sca-1 to work (using a rat anti-mouse clone from BD) using immunofluorescence - so am trying the Vector ABC system. Unfortunately, to get the sca-1 to work, I have to leave out triton-X - without the Triton-X the SPC seems not to be working. Does anyone have advice for trying to simultaneously stain for an intracytoplasmic protein and a membrane protein at the same time? Is there a better fixative than 4% formaldehyde ( we leave the tissues in formaldehyde from 6hours to no more than 48 hours). Many thanks - Melissa Mazan Melissa R. Mazan, DVM, Diplomate ACVIM Associate Professor and Director of Equine Sports Medicine Department of Clinical Sciences Tufts Cumming School of Veterinary Medicine 200 Westborough Road North Grafton, MA 01536 Tel:508-839-5395 Fax:508-839-7922 email: melissa.mazan@tufts.edu histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Milestone Pathos vs Sakura Xpress (Gregor Arlt) > 2. RE: Milestone Pathos vs Sakura Xpress (Jes Strong) > 3. Re: Milestone Pathos vs Sakura Xpress (Rene J Buesa) > 4. RE: Milestone Pathos vs Sakura Xpress > (Bartlett, Jeanine (CDC/CCID/NCZVED)) > 5. seeking hiotology position in DC area (Steven Wilkes) > 6. Re: Vison BioSystems - Peloris (Anthony Reilly) > 7. Hello, fellow histotechies!!! This is my first time posing a > question - so be gentle. Our patholog (Yvonne Jones) > 8. RE: Milestone Pathos vs Sakura Xpress > (Bartlett, Jeanine (CDC/CCID/NCZVED)) > 9. RE: Hello, fellow histotechies!!! This is my first time > posing a question - so be gentle. Our patholog > (rdavis4@rdg.boehringer-ingelheim.com) > 10. RE: Hello, fellow histotechies!!! This is my first time > posing a question - so be gentle. Our patholog (soofia siddiqui) > 11. heat antigen retrieval methods (Peter Rippstein) > 12. problems with mouse brain fixation (Martina Urbanek) > 13. Phospho-S6 Ribosomal Protein (Goodwin, Diana) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 16 Oct 2006 14:53:33 -0500 > From: "Gregor Arlt" > Subject: [Histonet] Milestone Pathos vs Sakura Xpress > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; format=flowed > > Dear Histonetters, > > I have heard the Pathos would use 600 Watts for the microwave processing. I > can't imagine that this is right. In my opinion this would destroy the RNS > structure of any tissue. On the other hand I heard tissues processed in the > Xpress would be easy to use for molecularbiological investigations. > > Has anybody experience with those instruments, or know anybody the power of > the microwave of the instruments. > > Thanks for the help Frank > > _________________________________________________________________ > Get FREE company branded e-mail accounts and business Web site from > Microsoft Office Live > http://clk.atdmt.com/MRT/go/mcrssaub0050001411mrt/direct/01/ > > > > > ------------------------------ > > Message: 2 > Date: Mon, 16 Oct 2006 15:43:55 -0500 > From: "Jes Strong" > Subject: RE: [Histonet] Milestone Pathos vs Sakura Xpress > To: > Message-ID: <00a501c6f163$cc5b0010$0200a8c0@Jes> > Content-Type: text/plain; charset="us-ascii" > > Dear Mr. Arlt, > > If you would like to contact Milestone directly, we would be happy to > explain the principles of Milestone's microwave processors including > magnetron output and how it is dynamically regulated by software to adjust > to each specific load. These are not questions that users would, or should > be expected to be able to answer for you satisfactorily. > > Jes Strong > Western Region Sales Manager > Milestone Medical > (203) 925-4240 (Office) > (847) 323-8373 (Cell) > (847) 655-6009 (Fax) > jes@milestonemed.com > > www.milestonemed.com > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gregor Arlt > Sent: Monday, October 16, 2006 2:54 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Milestone Pathos vs Sakura Xpress > > Dear Histonetters, > > I have heard the Pathos would use 600 Watts for the microwave processing. I > can't imagine that this is right. In my opinion this would destroy the RNS > structure of any tissue. On the other hand I heard tissues processed in the > Xpress would be easy to use for molecularbiological investigations. > > Has anybody experience with those instruments, or know anybody the power of > the microwave of the instruments. > > Thanks for the help Frank > > _________________________________________________________________ > Get FREE company branded e-mail accounts and business Web site from > Microsoft Office Live > http://clk.atdmt.com/MRT/go/mcrssaub0050001411mrt/direct/01/ > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 3 > Date: Mon, 16 Oct 2006 14:19:18 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Milestone Pathos vs Sakura Xpress > To: Gregor Arlt , > histonet@lists.utsouthwestern.edu > Message-ID: <20061016211918.92177.qmail@web61214.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Frank: > The temperature produced by the magnetron is "controlled" by the temperature probe adjusted to the processing protocol; you can have 1200W and a working temperature of 50?C so wattage in itself is not a deletereous agent just provides the capability of getting to high temperatures quickly, if needed.. > Not all technologies are alike and the Xpress uses 60W continuously in the first 2 chambers. PATHOS is pure microwave technology and Xpress is a blend of MW and conventional technology. > In the Xpress the first 2 chambers are identical and using MW technology and bubble agitation. The other 2 are just 2 conventional retorts with convection heat and vacuum capabilities, operated at 65?C. > The molecular integrity does not relate to the process but to the tissue fixation. Formalin greatly prevents RNA studies but any alcoholic fixative like Kryofix, BoonFix or the propietary by Sakura (UMFix) will preserve the macromolecules either if the tissue is going to be processed with MW or conventional technology. Any good alcoholic fixative containin PEG also will preserve the macromolecules. > Both PATHOS and Xpress are "walk away" instruments but Xpress allows for the continuous addition of up to 30 cassettes every 15 minutes, for an overall work flow of 120 cassettes after 105 minutes, and 30 more every 15 minutes afterwards. The limit is the thickness of the sections (have to be 1.5mm thick) and some tissues have to be previously fixed from 4 to 4.5 hours before processing. > PATHOS can process tissues of up to 5mm at a rate of 210 cassettes/4 hours (fixation included) for thick tissue slices or 210 cassettes/1 hour for small biopsies. > Hope this information will help you! > Ren? J. > > Gregor Arlt wrote: > Dear Histonetters, > > I have heard the Pathos would use 600 Watts for the microwave processing. I > can't imagine that this is right. In my opinion this would destroy the RNS > structure of any tissue. On the other hand I heard tissues processed in the > Xpress would be easy to use for molecularbiological investigations. > > Has anybody experience with those instruments, or know anybody the power of > the microwave of the instruments. > > Thanks for the help Frank > > _________________________________________________________________ > Get FREE company branded e-mail accounts and business Web site from > Microsoft Office Live > http://clk.atdmt.com/MRT/go/mcrssaub0050001411mrt/direct/01/ > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > All-new Yahoo! Mail - Fire up a more powerful email and get things done faster. > > ------------------------------ > > Message: 4 > Date: Mon, 16 Oct 2006 18:46:57 -0400 > From: "Bartlett, Jeanine \(CDC/CCID/NCZVED\)" > Subject: RE: [Histonet] Milestone Pathos vs Sakura Xpress > To: "Gregor Arlt" , > > Message-ID: > > Content-Type: text/plain; charset="UTF-8" > > Frank, > > I have no experience with the Pathos but our lab does have the Sakura Xpress. I am attaching a PDF of the brochure for the equipment. Page 4 explains how the microwave itself operates (60 watts). One thing I want to add to the previous response is that the 1.5 mm thickness for the Xpress is not mandatory. There is allowance for thicker specimens but the processing time is then lengthened. But I for one like having an excuse to make pathologists gross properly to begin with. :) > > Jeanine Bartlett > CDC, Atlanta > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Gregor Arlt > Sent: Mon 10/16/2006 3:53 PM > To: histonet@lists.utsouthwestern.edu > Cc: > Subject: [Histonet] Milestone Pathos vs Sakura Xpress > > > > Dear Histonetters, > > I have heard the Pathos would use 600 Watts for the microwave processing. I > can't imagine that this is right. In my opinion this would destroy the RNS > structure of any tissue. On the other hand I heard tissues processed in the > Xpress would be easy to use for molecularbiological investigations. > > Has anybody experience with those instruments, or know anybody the power of > the microwave of the instruments. > > Thanks for the help Frank > > _________________________________________________________________ > Get FREE company branded e-mail accounts and business Web site from > Microsoft Office Live > http://clk.atdmt.com/MRT/go/mcrssaub0050001411mrt/direct/01/ > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 5 > Date: Mon, 16 Oct 2006 22:15:25 -0400 > From: "Steven Wilkes" > Subject: [Histonet] seeking hiotology position in DC area > To: histonet@lists.utsouthwestern.edu > Message-ID: > <8e5827cf0610161915w1f450489mb6aec23f432807ae@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Hello > > I am seeking a histology position in the greater DC area. I have a MA in > biology, 2+ years of histology and immunohistochemistry experience, as well > as a bit histology teaching. Thank you > > Steven > > > ------------------------------ > > Message: 6 > Date: Tue, 17 Oct 2006 12:37:52 +1000 > From: "Anthony Reilly" > Subject: Re: [Histonet] Vison BioSystems - Peloris > To: , > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > Hi Richard > > My peloris was part of the very first relase of the instrument. As a > result there were some initial minor problems which have since been > rectified. As I said these problems were minor and never in any way > affected the processing of our tissue. > > It now runs very well and has had a positive impact on our laboratory. > The instrument has a very powerful mixing ability which not only > improves penetration but guarantees even heating on the steps where heat > is utilised giving shorter processing times even for fatty tissue. This > is aided by using one of their range of specimen baskets which separates > each cassette individually allowing better flow of solution to each > specimen. > > Our laboratory services both heart and lung transplant units requiring > us to run numeroous short cycles throughout the day. The improved > processing combined with a rapid clean cycle means that the one dual > retort peloris can do the work of 3-4 of our prevoius instruments. > > Examples of our improved times include: > > Fatty tissue 18h to 14h > Routine 12h to 9h > Small Biopsy 2h to 1 h > > This has also had an impact on IHC as the small biopsies that are > required urgently can be given an extra 1h in formalin and still be > completed in the same time as the previous protocol. With the faster > processing some tissues such as lletz biopsies need to be processed on > shorter cycles to avoid hardening of the tissue. According to the > manufacturer these times can be reduced further by substituting xylene > with isopropanol but I have not tried that so cannot comment. > > regards > > > > Tony Reilly > Chief Scientist > Anatomical Pathology > QHPS-Prince Charles Hospital > Rode Rd Chermside Q 4032 > Australia > Ph: 07 3139 4543 > Fax: 07 3193 4546 > tony_reilly@health.qld.gov.au > > > >>>> "Richard Cartun" 10/15/06 11:46 pm >>> >>>> > Anyone out there using Vison BioSystems' "Peloris" for tissue > processing? If so, what has been your experience? Thank you. > > Richard > > Richard W. Cartun, Ph.D. > Director, Immunopathology & Histology > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 > (860) 545-0174 Fax > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ***************************************************************** > This email, including any attachments sent with it, is > confidential and for the sole use of the intended recipient(s). > This confidentiality is not waived or lost, if you receive it and > you are not the intended recipient(s), or if it is transmitted/ > received in error. > > Any unauthorised use, alteration, disclosure, distribution or > review of this email is strictly prohibited. The information > contained in this email, including any attachment sent with > it, may be subject to a statutory duty of confidentiality if it > relates to health service matters. > > If you are not the intended recipient(s), or if you have > received this email in error, you are asked to immediately > notify the sender by telephone collect on Australia > +61 1800 198 175 or by return email. You should also > delete this email, and any copies, from your computer > system network and destroy any hard copies produced. > > If not an intended recipient of this email, you must not copy, > distribute or take any action(s) that relies on it; any form of > disclosure, modification, distribution and/or publication of this > email is also prohibited. > > Although Queensland Health takes all reasonable steps to > ensure this email does not contain malicious software, > Queensland Health does not accept responsibility for the > consequences if any person's computer inadvertently suffers > any disruption to services, loss of information, harm or is > infected with a virus, other malicious computer programme or > code that may occur as a consequence of receiving this > email. > > Unless stated otherwise, this email represents only the views > of the sender and not the views of the Queensland Government. > **************************************************************** > > > > > ------------------------------ > > Message: 7 > Date: Tue, 17 Oct 2006 02:43:21 -0400 > From: "Yvonne Jones" > Subject: [Histonet] Hello, fellow histotechies!!! This is my first > time posing a question - so be gentle. Our patholog > To: > Message-ID: <453443490200004F00000225@GWGATE1.ahm.com> > Content-Type: text/plain; charset="US-ASCII" > > Hello, fellow histotechies!!! This is my first time posing a question - > so be gentle. Our pathologists have recently requested that we begin > testing an antibody, p52. I have had a bear of a time finding any > information about this antibody!!! All I could find was one article > on-line, and I am still having a problem finding info and the antibody > itself. > > ----------------------------------------- > > > > > > ------------------------------ > > Message: 8 > Date: Tue, 17 Oct 2006 05:49:06 -0400 > From: "Bartlett, Jeanine \(CDC/CCID/NCZVED\)" > Subject: RE: [Histonet] Milestone Pathos vs Sakura Xpress > To: "Bartlett, Jeanine \(CDC/CCID/NCZVED\)" , "Gregor > Arlt" , > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Apparently the PDF was too large for Histonet to accept. You can go to > the Sakura website and click on Xpress and open the brochure if you need > these details. > > Jeanine > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Bartlett, Jeanine (CDC/CCID/NCZVED) > Sent: Monday, October 16, 2006 6:47 PM > To: Gregor Arlt; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Milestone Pathos vs Sakura Xpress > > Frank, > > I have no experience with the Pathos but our lab does have the Sakura > Xpress. I am attaching a PDF of the brochure for the equipment. Page 4 > explains how the microwave itself operates (60 watts). One thing I want > to add to the previous response is that the 1.5 mm thickness for the > Xpress is not mandatory. There is allowance for thicker specimens but > the processing time is then lengthened. But I for one like having an > excuse to make pathologists gross properly to begin with. :) > > Jeanine Bartlett > CDC, Atlanta > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > Gregor Arlt > Sent: Mon 10/16/2006 3:53 PM > To: histonet@lists.utsouthwestern.edu > Cc: > Subject: [Histonet] Milestone Pathos vs Sakura Xpress > > > > Dear Histonetters, > > I have heard the Pathos would use 600 Watts for the microwave > processing. I > can't imagine that this is right. In my opinion this would > destroy the RNS > structure of any tissue. On the other hand I heard tissues > processed in the > Xpress would be easy to use for molecularbiological > investigations. > > Has anybody experience with those instruments, or know anybody > the power of > the microwave of the instruments. > > Thanks for the help Frank > > > _________________________________________________________________ > Get FREE company branded e-mail accounts and business Web site > from > Microsoft Office Live > http://clk.atdmt.com/MRT/go/mcrssaub0050001411mrt/direct/01/ > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > ------------------------------ > > Message: 9 > Date: Tue, 17 Oct 2006 07:46:17 -0400 > From: rdavis4@rdg.boehringer-ingelheim.com > Subject: RE: [Histonet] Hello, fellow histotechies!!! This is my first > time posing a question - so be gentle. Our patholog > To: yjones2@csmlab.com, histonet@lists.utsouthwestern.edu > Message-ID: > <83BA2D3D42947D48BDAA449453644ABE0865B1@RDGEXM01.am.boehringer.com> > Content-Type: text/plain; charset=us-ascii > > Yvonne, > > Google p52 and lots of hits come up. Also, check out www.abcam.com. They > have p52 as a rabbit polyclonal. > > Rebecca A. Davis, A.A.S., NYS LVT, HT (ASCP) > Toxicology, Histopathology Lab > Boehringer-Ingelheim Pharmaceuticals, Inc. > rdavis4@rdg.boehringer-ingelheim.com > 203-798-5448 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yvonne Jones > Sent: Tuesday, October 17, 2006 2:43 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Hello, fellow histotechies!!! This is my first time > posing a question - so be gentle. Our patholog > > Hello, fellow histotechies!!! This is my first time posing a question - > so be gentle. Our pathologists have recently requested that we begin > testing an antibody, p52. I have had a bear of a time finding any > information about this antibody!!! All I could find was one article > on-line, and I am still having a problem finding info and the antibody > itself. > > ----------------------------------------- > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 10 > Date: Tue, 17 Oct 2006 07:39:31 -0700 (PDT) > From: soofia siddiqui > Subject: RE: [Histonet] Hello, fellow histotechies!!! This is my first > time posing a question - so be gentle. Our patholog > To: rdavis4@rdg.boehringer-ingelheim.com, yjones2@csmlab.com, > histonet@lists.utsouthwestern.edu > Message-ID: <20061017143931.16702.qmail@web39513.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Go to Google and go check for Biocompare.com. Biocompare is a very good source to search for any bio products. I have searched and found all of the antibodies, that Dako has discontinued, through this web site. Good luck! > Soofia > > rdavis4@rdg.boehringer-ingelheim.com wrote: > Yvonne, > > Google p52 and lots of hits come up. Also, check out www.abcam.com. They > have p52 as a rabbit polyclonal. > > Rebecca A. Davis, A.A.S., NYS LVT, HT (ASCP) > Toxicology, Histopathology Lab > Boehringer-Ingelheim Pharmaceuticals, Inc. > rdavis4@rdg.boehringer-ingelheim.com > 203-798-5448 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yvonne Jones > Sent: Tuesday, October 17, 2006 2:43 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Hello, fellow histotechies!!! This is my first time > posing a question - so be gentle. Our patholog > > Hello, fellow histotechies!!! This is my first time posing a question - > so be gentle. Our pathologists have recently requested that we begin > testing an antibody, p52. I have had a bear of a time finding any > information about this antibody!!! All I could find was one article > on-line, and I am still having a problem finding info and the antibody > itself. > > ----------------------------------------- > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Get your email and more, right on the new Yahoo.com > > ------------------------------ > > Message: 11 > Date: Tue, 17 Oct 2006 11:36:56 -0400 > From: "Peter Rippstein" > Subject: [Histonet] heat antigen retrieval methods > To: > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > Hello Histonetters, > > Our lab is in need of some information in regards heat antigen > retrieval methods. Has anyone done a comparison in terms of results & > cost effectiveness obtained from microwave irradiation vs pressure > cooking and steam heating methods. Any recommendations would be > appreciated. Many thanks. > Peter > > > Peter Rippstein ART, MLT > Core Pathology Laboratory > Rm H2102 > University of Ottawa Heart Institute > 40 Ruskin Street > Ottawa, Ontario > Canada, K1Y 4W7 > > Tel: (613) 761-5282 > Fax: (613) 761-5281 > Email: prippstein@ottawaheart.ca > > > > > > > -------------- next part -------------- > BEGIN:VCARD > VERSION:2.1 > X-GWTYPE:USER > FN:Peter Rippstein > EMAIL;WORK;PREF;NGW:PRippstein@ottawaheart.ca > N:Rippstein;Peter > END:VCARD > > BEGIN:VCARD > VERSION:2.1 > X-GWTYPE:USER > FN:Peter Rippstein > EMAIL;WORK;PREF;NGW:PRippstein@ottawaheart.ca > N:Rippstein;Peter > END:VCARD > > BEGIN:VCARD > VERSION:2.1 > X-GWTYPE:USER > FN:Peter Rippstein > EMAIL;WORK;PREF;NGW:PRippstein@ottawaheart.ca > N:Rippstein;Peter > END:VCARD > > BEGIN:VCARD > VERSION:2.1 > X-GWTYPE:USER > FN:Peter Rippstein > EMAIL;WORK;PREF;NGW:PRippstein@ottawaheart.ca > N:Rippstein;Peter > END:VCARD > > > ------------------------------ > > Message: 12 > Date: Tue, 17 Oct 2006 17:51:41 +0200 > From: Martina Urbanek > Subject: [Histonet] problems with mouse brain fixation > To: histonet@lists.utsouthwestern.edu > Message-ID: <1161100301.4534fc0d23f8a@web-mail1.uibk.ac.at> > Content-Type: text/plain; charset=ISO-8859-1 > > Hello everybody on histonet, > > we have some problems with handling mouse (21 and 90 days old) and rat brains. > We do mouse brain perfusion using 4% paraformaldehyde in PBS, pH 7.4. We use > gravity for perfusion for about 30 minutes (volume about 80 ml) and after > perfusion we leave the brains over night in the same fixative. Then we process > in a Shandon tissue processor (70% Alcohol, 80% Alcohol, 95% Alcohol, 3 changes > 100% alcohol, 3 changes xylene, 2 changes paraffin 56?C; time depends on size > of tissue). Now we have the problem that some brains are too hard and some also > seem to shrink more than others, when we cut them and put them on waterbath > they seem to expand and distort and often brittle. So that it looks like only > fibrous tissue is left, the structure is gone. I already had a look on histonet > archive but did not find anything that helps, therefore I hope that someone has > an idea what can be wrong. I have to say that we don?t have any problems when > we immersion fix the brains with 4% formaldehyde. > The problems we have with rat brains are a bit different, because we get the > brains from another group who perfuse the lung with 4% paraformaldehyde in > Hepes-buffer, pH 7.35. We then postfix over night in the same fixative they > use (when the brain is also perfused) or for 3 days (when brain is not > perfused). After dehydration in tissue processor we have the same cutting > problems like we observe with mouse brain tissue, even worse. > Any suggestions are appreciated! > > Thank you very much for your help!!! > > Martina Urbanek > > > > Ms. Martina Urbanek > Forschungslabor der > Klin.Abt. f?r Neonatologie > neonatal neuroscience research laboratory > Med. University Innsbruck > Innrain 66, 4th floor > A-6020 Innsbruck > Tel. +43 (0)512 504 27755/27765 > Fax: +43 (0)512 504 27766 > Email: Martina.Urbanek@uibk.ac.at > > > > > > > > > > > > ------------------------------ > > Message: 13 > Date: Tue, 17 Oct 2006 12:52:24 -0400 > From: "Goodwin, Diana" > Subject: [Histonet] Phospho-S6 Ribosomal Protein > To: , > > Message-ID: > <80CDD9C3FEEAFD4982B114C4A6DFD00E02CB8235@uphsmbx2.UPHS.PENNHEALTH.PRV> > > Content-Type: text/plain; charset="us-ascii" > > For those of us 'Netters using this Ab on human tissue, which one and at > what dilution? > > Thanks! > > Diana Goodwin > Supervisor, Anatomic Pathology > Pennsylvania Hospital > Preston 655-C > ph. 215-829-6532 > pager 215-422-5160 > fax 215-829-7564 > e-mail goodwind@[pahosp.com > > > > The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 35, Issue 28 > **************************************** > ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 44, Issue 18 **************************************** From gagnone <@t> KGH.KARI.NET Tue Jul 17 13:59:31 2007 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Tue Jul 17 14:04:48 2007 Subject: [Histonet] Red Box Slides Followup Message-ID: Thanks to everyone for their contribution of experiences with the Red Box control slides. We will try the Thermo Fisher etched-box slides. It sounds as if there may be factors linked to the reagents dispensing onto the slides. It's one of those nebulous areas that occur with staining results in some cases...is it instrument-related, slide-related or a little bit of both? The box for the control tissue is useful in that the pathologist knows exactly where to look, and there's less chance of mistaking control tissue for patient tissue. Of interest, Thermo Fisher Scientific is working with Ventana to develop a Red Box of a design better optimized to the Benchmark. Thanks again, Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada From Rcartun <@t> harthosp.org Tue Jul 17 14:52:17 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Jul 20 12:34:14 2007 Subject: [Histonet] Babesiosis Message-ID: <469CE5B10200007700006ED7@gwmail.harthosp.org> Anyone interested in Babesiosis? We may have a ruptured spleen due to Babesiosis. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From cwscouten <@t> myneurolab.com Tue Jul 17 17:37:23 2007 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Sun Jul 22 04:15:52 2007 Subject: [Histonet] Clinical cryostat info needed References: <20070712120032635.00000005664@spikey> Message-ID: <5784D843593D874C93E9BADCB87342AB0395E673@tpiserver03.Coretech-holdings.com> Vibratome company also offers a self decontaminating cryostat. It used Peracetic acid, and works like a dishwasher to splash it everywhere, then rinse it away. http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=475102&catdesc=Histology+Equipment&CatThreeID=732&CatOneID=4&subcatdesc=Cryostats&idsubcategory=182 Cordially, Charles W.? Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 x 342 FAX? 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mickie Johnson Sent: Thursday, July 12, 2007 8:07 PM To: 'Lesley Bechtold'; 'Histology Network' Subject: RE: [Histonet] Clinical cryostat info needed Hi Leslie, The Leica 1850 UV has a UV light for decontamination. It is a great cryostat and easy to use. There are other brands out there some of which have decontamination capability. I'm sure Micron and Thermo/Shandon models do too. The Leica is the most ergonomic and the right height for the average person (5'2" to 5'10") with little stooping over. Use a 2.5 degree knife angle instead of 5 degrees it comes set at. Make sure the knife holder back plate screws and lower horizontal plate screws (also on the knife holder) are good and tight, when you first get it in. I have had chatter because of this a couple of times. Their anti-roll plate when adjusted correctly is the bomb. I have converted several avid 'I only use a brush' individuals. I set up Mohs labs and train Mohs technicians every week and they love Leica! Good Luck. Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lesley Bechtold Sent: Thursday, July 12, 2007 9:01 AM To: Histology Network Subject: [Histonet] Clinical cryostat info needed Hi, I need to provide information and approximate pricing for a clinical cryostat - one with a decontamination system. I would welcome responses from vendors but any info on the best model out there - particularly any models that are user-friendly for a wide range of potential users - would be welcome. Thank you Lesley Lesley S. Bechtold Senior Manager, Histopathology & Microscopy Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sqd3f <@t> cms.mail.virginia.edu Tue Jul 17 21:33:24 2007 From: sqd3f <@t> cms.mail.virginia.edu (Sonny Duong) Date: Sun Jul 22 04:18:43 2007 Subject: [Histonet] Unsuccessful Muscle Lipid Staining Message-ID: Hi all, I have been trying to stain mouse muscle tissue for intramuscular triglyceride droplets with Oil Red O (dissolved in triethyl phosphate) for some time now, and my results have been disastrous. I've tried it on both fresh frozen and formaldehyde fixed tissues (and cryoprotected) cryosections, but almost always it seems that the lipid droplets within the fibers (maybe?) leak out of the cells and conglomerate on the edges of the tissue and within gaps between the cells. Very few of the fibers, if any, display any lipid droplet staining and it is always very weak. The lipid droplets all conglomerate in roughly the same positions between serial sections as well, which leads me to believe something is happening during the freezing/fixation process, or the cutting in the cryosection. Does anyone have any suggestion as to what I could be doing wrong here (i.e cutting technique, temperature, fixatives)? Also, does anyone have any experience with using ORO in triethyl phosphate? Thank you all for your time, Sonny Duong University of Virginia Green Lab From garret.t.miyamoto <@t> us.army.mil Tue Jul 17 22:14:59 2007 From: garret.t.miyamoto <@t> us.army.mil (garret.t.miyamoto@us.army.mil) Date: Sun Jul 22 04:21:42 2007 Subject: [Histonet] Re: Histonet Digest, Vol 43, Issue 39 In-Reply-To: <5s9rag$taeik@mxoutps1.us.army.mil> References: <5s9rag$taeik@mxoutps1.us.army.mil> Message-ID: Re: QC sheets for routine cutting and H&E staining Deanna, I will fax you a copy of the QC sheet that we use in our lab. Hope it will be good for you. Garret Miyamoto Histotechnician, Tripler Army Medical Center ----- Original Message ----- From: histonet-request@lists.utsouthwestern.edu Date: Friday, June 29, 2007 7:04 am Subject: Histonet Digest, Vol 43, Issue 39 To: histonet@lists.utsouthwestern.edu > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. QC sheets for routine cutting and H&E Staining > (Snider, Vivian Deanna) > > > ------------------------------------------------------------------- > --- > > Message: 1 > Date: Fri, 29 Jun 2007 11:29:35 -0400 > From: "Snider, Vivian Deanna" > Subject: [Histonet] QC sheets for routine cutting and H&E Staining > To: > Message-ID: > <84BE46B37B314D409C5A17B7BAB022D60153389D@IDC-EX-VS01.shriners.cc> > Content-Type: text/plain; charset="us-ascii" > > Does anybody have a standard QC sheet for tracking routine cutting and > staining of tissue that they could fax or email me? I could make > one in > Excel, but quite frankly just do not have the time! > > Thanks in advance, > > Deanna Snider > > Lead Tech > > Shriners Hospital for Children > > Cincinnati, OH > > 513-872-6388 > > 513-872-6299 fax > > > > CONFIDENTIALITY NOTICE: This e-mail communication and any > attachments may contain confidential and privileged information > for the use of the designated recipients. If you are not the > intended recipient, (or authorized to receive for the recipient) > you are hereby notified that you have received this communication > in error and that any review, disclosure, dissemination, > distribution or copying of it or its contents is prohibited. If > you have received this communication in error, please destroy all > copies of this communication and any attachments and contact the > sender by reply e-mail or telephone (813) 281-0300. > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 43, Issue 39 > **************************************** > From garth <@t> apollosci.co.za Wed Jul 18 01:39:00 2007 From: garth <@t> apollosci.co.za (Garth Jerome) Date: Sun Jul 22 04:21:55 2007 Subject: [Histonet] Microwave v conventional processing In-Reply-To: <7A885E8FE1C71C488D974EC601FAA690019E942B@vla-exchn1.cvlnt.vla.gov.uk> Message-ID: <000401c7c906$58c43970$7700a8c0@jhb.apollosci.co.za> Hello Colin We recently ran an exercise for the University of Pretoria, Veterinary Faculty. We processed a wide range of animal tissues, including, believe it or not crocodile tissues. These tissues were processed in a Milestone RHS-2 / Histos 3 histoprocessor. The results were excellent and in some cases we obtained better morphology on tissues than the current conventional processing. If you would like contact details for the persons required I can happily assist you. Best regards, Garth Jerome Apollo Scientific cc Telephone : 27-11-466 7666 Fascimile : 27-11-466 7672 Email : garth@apollosci.co.za -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weaver, Colin Sent: 17 July 2007 05:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microwave v conventional processing Hi - we are trying to go down the microwave route in processing but inevitably some of our veterinary pathologists are questioning whether microwave sections are as "good" as conventional processing. Can anyone point me in the right direction to find any comparison done between microwave processing and conventional overnight processing with regard to section and staining quality. Colin Weaver Veterinary Laboratories Agency (VLA) This email and any attachments is intended for the named recipient only. If you have received it in error you have no authority to use, disclose, store or copy any of its contents and you should destroy it and inform the sender. Whilst this email and associated attachments will have been checked for known viruses whilst within VLA systems we can accept no responsibility once it has left our systems. Communications on VLA's computer systems may be monitored and/or recorded to secure the effective operation of the system and for other lawful purposes. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Wed Jul 18 09:28:19 2007 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Sun Jul 22 04:55:04 2007 Subject: [Histonet] Kappa and Lambda from Vision Biosystems (Novocastra) Message-ID: Good morning, We recently received samples of these two antibodies (clones KP-53 and HP-6054 respectively). Since we put our antibodies in dispensers to run on our Ventana automated stainers I'm concerned about the stipulation on the data sheet to "prepare working dilutions on the day of use". How are others handling this situation? Is the stability actually better than one might be led to believe by this caveat? Thanks for your responses, Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From minniesann <@t> hotmail.com Wed Jul 18 09:42:44 2007 From: minniesann <@t> hotmail.com (Maribel Santiago) Date: Sun Jul 22 04:55:07 2007 Subject: [Histonet] Questions? Message-ID: Hi everyone, 1) I need help to find a commercial hematoxylin that I can use for GMA, paraffin, frozen and cytospins H&E's and I don't need to filter everyday. I recently bought Harris hematoxylin,acidified (mercury free) from Polysciences, Inc. I don't like it. Does anyone out there has a suggestion? 2) Does anyone out there using tunnel assays? Which is the best tunnel for IHC? 3) Also I need to learn EM where can I learn it? What schools offer EM? I live In Washington,DC area. Thanks so much in advance, Minnie _________________________________________________________________ With Windows Live Hotmail, you can personalize your inbox with your favorite color. www.windowslive-hotmail.com/learnmore/personalize.html?locale=en-us&ocid=TXT_TAGLM_HMWL_reten_addcolor_0607 From gcallis <@t> montana.edu Wed Jul 18 10:10:44 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Sun Jul 22 04:55:12 2007 Subject: [Histonet] Re: Phosphorylation and IHC In-Reply-To: References: <6.0.0.22.1.20070516105917.01b39d10@gemini.msu.montana.edu> Message-ID: <6.0.0.22.1.20070718085445.01b39358@gemini.msu.montana.edu> Roche has PhosStop which should be added to the fixative and to all other buffers to prevent dephosphorylation in formalin fixed tissue sections for immunohistochemical staining. They will send you a free sample. It is called PhosStop, a phosphatase inhibitor cocktail tablet. Catalog # is 04 906 845 001 for 10 tablets, and they have 20 tablet packages too. www.roche-applied-science.com At 01:06 PM 7/17/2007, you wrote: >Hi, >I know phosphoylation was kind of a hot topic at the last NSH. I will >have to start looking at phosphoylation states in the near future. Do you >have any suggestions? >IHC on rat tisssues- spinal cord, brain and possibly dorsal root ganglia. > >Is perfusion or fixation preferred. >What about frozen, how should the tissue be frozen. >Any suggestion about buffers? Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From minniesann <@t> hotmail.com Wed Jul 18 10:21:17 2007 From: minniesann <@t> hotmail.com (Maribel Santiago) Date: Sun Jul 22 04:55:13 2007 Subject: [Histonet] Questions? Message-ID: Hi everyone, 1) I need help to find a commercial hematoxylin that I can use for GMA, paraffin, frozen and cytospins H&E's and I don't need to filter everyday. I recently bought Harris hematoxylin,acidified (mercury free) from Polysciences, Inc. I don't like it. Does anyone out there has a suggestion? 2) Does anyone out there using tunnel assays? Which is the best tunnel for IHC? 3) Also I need to learn EM where can I learn it? What schools offer EM? I live In Washington,DC area. Thanks so much in advance, Minnie Make every IM count. Download Windows Live Messenger and join the i'm Initiative now. It's free. Make it count! _________________________________________________________________ Express yourself instantly with MSN Messenger! Download today it's FREE! http://messenger.msn.click-url.com/go/onm00200471ave/direct/01/ From tkngflght <@t> yahoo.com Wed Jul 18 10:22:13 2007 From: tkngflght <@t> yahoo.com (Cheryl) Date: Sun Jul 22 04:55:26 2007 Subject: [Histonet] FL licensed Histotech In-Reply-To: <013b01c7d44c$584776f0$d49eae18@yourxhtr8hvc4p> Message-ID: <249645.47051.qm@web50912.mail.re2.yahoo.com> Hi, Seeking a Florida licensed Histotech for a temporary position - Day Shift with the length of the contract being negotiable. Thank you, Chery Kerry, HT(ASCP) (800) 756-3309 From plott <@t> uab.edu Wed Jul 18 12:49:14 2007 From: plott <@t> uab.edu (Patricia F Lott) Date: Sun Jul 22 05:07:21 2007 Subject: [Histonet] processing samples with bone cement Message-ID: <4850136AECAE5447B2E7FF80CA9225300C3177@UABEXMB4.ad.uab.edu> Does anyone have a protocol for processing samples with bone cement into plastic? We need to retain the bone cement! From ROrr <@t> enh.org Wed Jul 18 13:43:03 2007 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Sun Jul 22 05:09:30 2007 Subject: [Histonet] cytokeratin question Message-ID: Sylinda, Depending on the tissue type, look into the following CK7 CK20 (or CDX2) CK 5/6 HMW CK (34beta E 12) Hope this helps. Becky Orr CLA,HT(ASCP)QIHC Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 From: bsylinda@aol.com Subject: [Histonet] CYTOKERATIN QUESTION To: histonet@lists.utsouthwestern.edu Hello Histoland, Can anyone in histoland inform me of the best cytokeratin antibody to use to differenate squamous cell carcinoma and adenocarcinoma.? Any feedback would be greatly appreciated. Thanks in advance, Sylinda From jbrod <@t> tvmdl.tamu.edu Wed Jul 18 13:44:40 2007 From: jbrod <@t> tvmdl.tamu.edu (Jordan Brod) Date: Sun Jul 22 05:09:32 2007 Subject: [Histonet] H&E Stain for Linear Stainer... Message-ID: <20070718T134440Z_3035000D0000@tvmdl.tamu.edu> I am trying to develop our H&E stain. We have a linear stainer from Hacker. I would like to look at some other H&E Procedures. If you don't mind and you have a linear stainer, please send me your procedure. Thanks, Jordan From Debra.Reade <@t> lhsc.on.ca Wed Jul 18 14:22:34 2007 From: Debra.Reade <@t> lhsc.on.ca (Debra Reade) Date: Sun Jul 22 05:14:20 2007 Subject: [Histonet] background staining on flourescent slides Message-ID: <469E303A020000BD0000A0E2@lhscgwiao.lhsc.on.ca> Has anyone experienced background flourescense with Primate Pancreas Slides from IMMCO Diagnostics? When doing ICA's we have recently experienced so much background (dirty flecks) that they are unreadable. Any suggestions?? We have tried using new secondary antibody with no difference. From rosenfeldtek <@t> hotmail.com Wed Jul 18 17:55:35 2007 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Sun Jul 22 05:18:50 2007 Subject: [Histonet] Ripples in Arterial sections Message-ID: I am cutting 5 micron sections from formalin fixed, paraffin embedded segments of rabbit abdominal aorta. I float my ribbons in a warm water bath then pick them up with a Superfrost Plus slide. When I check my sections uner the microscope, the sections have a wavy, or rippled appearance--It is not really "wrinkles, in the sense of the paraffin folding and sticking to itself--it is as if the arterial segment is contracting after being cut. It looks like a circular roadway that dips down then up, then down, then up... Has anyone ever seen this artifact with arterial sections? Any ideas abiut why uit happens and what I could do to prevent it? Thanks Jerry L. Ricks Research Scientist U.W. Medicine at South Lake Union 815 Mercer Street Seattle, WA 98109 (206)-685-7190 _________________________________________________________________ [1]See what youre getting intobefore you go there References 1. http://g.msn.com/8HMAENUS/2734??PS=47575 From Asf2k3 <@t> aol.com Wed Jul 18 18:05:16 2007 From: Asf2k3 <@t> aol.com (Asf2k3@aol.com) Date: Sun Jul 22 05:18:52 2007 Subject: [Histonet] Re: Histonet Digest, Vol 44, Issue 18 Message-ID: Any open positions for Histo Tech in GA? ************************************** Get a sneak peek of the all-new AOL at http://discover.aol.com/memed/aolcom30tour From abarral <@t> nereuspharm.com Wed Jul 18 18:36:12 2007 From: abarral <@t> nereuspharm.com (Ana M. Barral) Date: Sun Jul 22 05:18:53 2007 Subject: [Histonet] please unsusbcribe In-Reply-To: References: Message-ID: <5C51E14B2B7FF14890F4EA489C302C7201D3702D@npserver.nereuspharm.com> Ouf of office until 8/2. Thanks, Ana From mbmphoto <@t> gmail.com Wed Jul 18 22:40:25 2007 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Sun Jul 22 05:21:37 2007 Subject: [Histonet] need protocol for poly-L-Lysine subbing Message-ID: <41E75B64-A438-4F22-A4DC-13A064C66CF8@gmail.com> Could someone please send me a a working protocol for making poly-L- Lysine solution so I can sub coat 50x75mm glass slides. Please include from whom you purchase the sub solution. Many thanks in advance for your time and help. Maria Bartola Mejia UCSF Department of Neurosurgery SF CA 94103 From Tanni.Ahmed <@t> intervet.com Thu Jul 19 03:03:15 2007 From: Tanni.Ahmed <@t> intervet.com (Ahmed, T (Tanni)) Date: Sun Jul 22 05:21:46 2007 Subject: [Histonet] CYTOKERATIN QUESTION Message-ID: Hello Histoland, Can anyone in histoland inform me of the best cytokeratin antibody to use to differenate squamous cell carcinoma and adenocarcinoma.? Any feedback would be greatly appreciated. Thanks in advance, Sylinda ________________________________ Hi Sylinda, The best cytokeratin markers are either CK5/6 or CK7 for differentiating squamous cell carcinoma and adenocarcinoma. The immunohistochemical profiling panel usually consists of: CK7 -ve, CK5/6 +ve, CK20 -ve and TTF1 -ve for squamous cell carcinoma CK7 +ve, CK5/6 -ve, CK20 -ve and TTF1 +ve for adenocarcinoma Hope this helps, Tanni Tanni S Ahmed Histopathology, R&D Intervet UK Ltd E-mail: tanni.ahmed@intervet.com -------------------------------------- This message, including attachments, is confidential and may be privileged. If you are not an intended recipient, please notify the sender then delete and destroy the original message and all copies. You should not copy, forward and/or disclose this message, in whole or in part, without permission of the sender. -------------------------------------- From JCarpenter764 <@t> aol.com Thu Jul 19 06:47:37 2007 From: JCarpenter764 <@t> aol.com (JCarpenter764@aol.com) Date: Sun Jul 22 05:24:21 2007 Subject: [Histonet] just a test Message-ID: ************************************** Get a sneak peek of the all-new AOL at http://discover.aol.com/memed/aolcom30tour From JCarpenter764 <@t> aol.com Thu Jul 19 07:15:23 2007 From: JCarpenter764 <@t> aol.com (JCarpenter764@aol.com) Date: Sun Jul 22 05:24:23 2007 Subject: [Histonet] please help i am not receiving mails from the histonet??? Message-ID: ************************************** Get a sneak peek of the all-new AOL at http://discover.aol.com/memed/aolcom30tour From cdemarinis <@t> SARATOGACARE.ORG Thu Jul 19 08:44:52 2007 From: cdemarinis <@t> SARATOGACARE.ORG (Demarinis, Carolyn) Date: Sun Jul 22 05:35:58 2007 Subject: [Histonet] inventory Message-ID: Does anyone have any suggestions for improving the inventory process for ordering and monitoring supplies for both anatomic and clinical laboratory? I would like to purchase software to improve the management of our inventory. Any suggestions? Thanks. From jcarpenter764 <@t> aol.com Thu Jul 19 09:39:43 2007 From: jcarpenter764 <@t> aol.com (jcarpenter764@aol.com) Date: Sun Jul 22 05:42:39 2007 Subject: [Histonet] please help Message-ID: <8C99829C7D029C3-F10-3@FWM-M37.sysops.aol.com> For some reason or another i am not receiving my histonet mail. I have not unsubscribed. Can you tell what is wrong. Thanks Jennell ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From mward <@t> wfubmc.edu Thu Jul 19 10:42:13 2007 From: mward <@t> wfubmc.edu (Martha Ward) Date: Sun Jul 22 05:44:51 2007 Subject: [Histonet] LYVE-1 and VEGFR-3 antibodies Message-ID: <61135F0455D33347B5AAE209B903A3041C372FCA@EXCHVS2.medctr.ad.wfubmc.edu> I have been asked by my Dermatopathologist to work up these two antibodies. Could someone point me in the direction of a vendor for these, and also dilutions, etc? Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center Medical Center Blvd. Winston-Salem, NC 27157 336-716-2756 mward@wfubmc.edu From HornHV <@t> archildrens.org Thu Jul 19 11:16:26 2007 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Sun Jul 22 05:44:52 2007 Subject: [Histonet] vials for autostainer Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB7254@EMAIL.archildrens.org> I remember someone on this list saying there were vials for the dako autostainer by another company that were cheaper. Will someone send that information? Thanks so much!! Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From MBURTON1 <@t> PARTNERS.ORG Thu Jul 19 11:28:28 2007 From: MBURTON1 <@t> PARTNERS.ORG (Burton, Mark) Date: Sun Jul 22 05:44:54 2007 Subject: [Histonet] collagen measurement Message-ID: Hello, A colleague of mine is trying to measure collagen in mouse skin. Can anyone recommend a quantitative or semi-quantitative method to do this? Thank you. Mark Burton HTL ASCP Brigham & Women's Hospital Boston, MA The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From MMargiotta <@t> bmhmc.org Thu Jul 19 12:54:27 2007 From: MMargiotta <@t> bmhmc.org (Margiotta, Michele) Date: Sun Jul 22 05:45:11 2007 Subject: [Histonet] test Message-ID: <922CE5B88F398948B4076A9A4340E7AF03411B0E@bmh_exchange.bmhmc.org> Haven't gotten any e-mails? Just checking. This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. From tkngflght <@t> yahoo.com Thu Jul 19 19:34:32 2007 From: tkngflght <@t> yahoo.com (Cheryl) Date: Sun Jul 22 05:52:40 2007 Subject: [Histonet] Question about Histology OJT facilities In-Reply-To: <661949901A768E4F9CC16D8AF8F2838CA1C672@IS-E2K3.grhs.net> Message-ID: <329858.93826.qm@web50906.mail.re2.yahoo.com> Hi All-- A couple of people have asked if there are still facilities that train histologist in-house starting the trainee out as an assistant and teaching them while they take classes through an accredited program like Hartford or IUPUI. I know these exist but with all the recent changes in licensure, I'm sure things have changed. If you could respond with this information about any facility you know that has an in-house On The Job training program either affiliated with a school or not, I'll pass it on. This is a more and more frequent question and as not all OJT programs are NCCLS accredited, they can be hard to locate. We appreciate your help in advance! Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab, one great tech at a time! 800.756.3309 From wilson_c <@t> ricerca.com Fri Jul 20 09:16:11 2007 From: wilson_c <@t> ricerca.com (Wilson, Carol) Date: Sun Jul 22 06:07:59 2007 Subject: [Histonet] Provantis software - Research (CRO) - Trimming station set up Message-ID: <9D443EB9D0270143B5AAF190CB1A58A305064E73@dogwood.ricerca.com> Hi All, Is there anyone out there using the Provantis software? If so, is it a very useful system in the actual histology lab? Also, for those folks with trimming stations, would you not say that they would require a sink, running water, direct lighting at bare minimum? I'm trying to get through to those who designed my new lab that a counter with some nice ventilation is a good start, but needs some improvements. Thanks in advance, and for those who followed my earlier postings... I've made the switch and am trying to get the hang of my new research lab. Carol Carol Wilson Team Leader - Histology Ricerca Biosciences, LLC From donna <@t> milestonemed.com Fri Jul 20 10:25:12 2007 From: donna <@t> milestonemed.com (Donna Willis) Date: Sun Jul 22 06:12:31 2007 Subject: [Histonet] Test Message-ID: <002701c7cae2$2fc46c10$8decd9a6@LAPTOP02> Is there problems with the histonet? Donna Willis,HT(ASCP)HTL Milestone Medical North American Application Manager 2100 N. Hwy 360 Suite 506 Grand Prairie, Tx 75050 972-606-9986 office 214-725-6184 cell From yvan_lindekens <@t> yahoo.com Fri Jul 20 10:26:09 2007 From: yvan_lindekens <@t> yahoo.com (yvan lindekens) Date: Sun Jul 22 06:12:34 2007 Subject: [Histonet] Spanish pioneers on histology Message-ID: <526121.2696.qm@web30911.mail.mud.yahoo.com> Hi all, Spain has some illustrous pioneers on histological technique. I suppose Pio Del Rio Orega and Ramon Y Cajal are the most famous amongst them. As we are about to take off for a weeks holliday in Madrid, I was wondering if someone could recomend some musea to learn something on histology/microscopy in Spain... If there would be Spanish microscopists wanting to meet: we (my girlfriend, my lovely baby daughter and I) will probably spending the evenings at the Plaza de Santa Ana, Madrid. My girlfriend and me, we'll drinking ice chilled Cointreau there. It's some kind of a habbit. Or an addiction. Or something in between :-))). You're welcome to join us! Thanks in advance for every advice you can give on Spanish microscopical technique! Yvan. ____________________________________________________________________________________ Boardwalk for $500? In 2007? Ha! Play Monopoly Here and Now (it's updated for today's economy) at Yahoo! Games. http://get.games.yahoo.com/proddesc?gamekey=monopolyherenow From TownsendD <@t> childrensdayton.org Fri Jul 20 10:31:03 2007 From: TownsendD <@t> childrensdayton.org (Dolores Townsend) Date: Sun Jul 22 06:12:37 2007 Subject: [Histonet] Problems with the Histonet? Message-ID: I have not received any message from the Histonet since Wednesday morning. Anyone else having this problem? Dolores From rjbuesa <@t> yahoo.com Fri Jul 20 10:45:46 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Jul 22 06:15:28 2007 Subject: [Histonet] MWO validation. In-Reply-To: <004401c7cae3$e7f616c0$6801a8c0@Patsy> Message-ID: <484779.21679.qm@web61221.mail.yahoo.com> Hi Patsy: I completely agree with you. A few days ago I advised an MD do to hold using MWO for breast that was going to be used with IHC tests. My argument was as follows: 1- FDA has approved Her2Neu for FFPE tissues, and they say NOTHING about MWO although it could be assumed that it was using conventional processig 2-DAKO developed their protocol (later approved by FDA) using conventional tissue processors 3- IF something happens and a lawsuit is brought, any savy lawyer (and they are savy enough) could "dig-out" the type of processing used and IF it was MWO it is likely that their argument could be accepted by a jury. I don't think that a lab individual validaiton could hold in court (against an FDA approved procedure). NEW guidelines by the FDA would be needed (as you point out). MWO users beware! Ren? J. Patsy Ruegg wrote: Rene, Has mw processing been validated for IHC (especially for her2neu) by running 25-100 cases side by side (one piece conventionally processed and the other mw processed from each of the 25-100 cases) then running the IHC? Until this is done and reported the new guidelines for her2 testing will require that any deviation from conventional processing and fixation for 6-48hr using the FDA approved her2neu kits, labs using mw processing will not be in compliance. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, July 17, 2007 10:17 AM To: Weaver, Colin; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Microwave v conventional processing Colin: Using the adequate protocols MW processing renders equivalent results to "conventional" tissue processing, that is the general concensus. The thing is that unless you use an automated MW tissue processor, a histotech will have to attend to the process and change reagents manually. This can lead to 2 problems: higher exposure of the HT to (usually hot) chemicals and some degree of inconsistency in the protocol because the time in each reagent could vary slightly different between runs. Consider that a few minutes in a conventional protocol is a much lower percentage of the time in the reagent, than the same amount of time in a much faster protocol completed with a MW tissue processor. MW processing should be an option when TAT is an issue and even then there are numerous manual steps independent of the time the tissue is involved in the processing step; they are independent of the processing technology and usually count for the greater part of the total TAT. Under separate cover I am sending you an article of mine wher I analyze this issue. Ren? J. "Weaver, Colin" wrote: Hi - we are trying to go down the microwave route in processing but inevitably some of our veterinary pathologists are questioning whether microwave sections are as "good" as conventional processing. Can anyone point me in the right direction to find any comparison done between microwave processing and conventional overnight processing with regard to section and staining quality. Colin Weaver Veterinary Laboratories Agency (VLA) This email and any attachments is intended for the named recipient only. If you have received it in error you have no authority to use, disclose, store or copy any of its contents and you should destroy it and inform the sender. Whilst this email and associated attachments will have been checked for known viruses whilst within VLA systems we can accept no responsibility once it has left our systems. Communications on VLA's computer systems may be monitored and/or recorded to secure the effective operation of the system and for other lawful purposes. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Fussy? Opinionated? Impossible to please? Perfect. Join Yahoo!'s user panel and lay it on us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Need a vacation? Get great deals to amazing places on Yahoo! Travel. From igor.deyneko <@t> gmail.com Fri Jul 20 10:46:06 2007 From: igor.deyneko <@t> gmail.com (Igor Deyneko) Date: Sun Jul 22 06:15:30 2007 Subject: [Histonet] Shandon Sequenza(r) Immunostaining Center Message-ID: <35e16a770707200846v4c873163v6cbda858caa93950@mail.gmail.com> Hello. I was just wondering whether or not someone uses Shandon Sequenza(r) Immunostaining Center for IHC? The reason i am asking is that do you use it for all your IHC or in any specific cases. In my experience, I found that 2 slices of tissue on the same slide get different amount of reagents, so when viewed under the microscope, they light up differently, showing different levels of saturation with a chromogen. Thank you. Igor Deyneko. Infinity Pharmaceutical Cambridge,MA. From llewllew <@t> shaw.ca Fri Jul 20 11:42:50 2007 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Sun Jul 22 06:15:31 2007 Subject: [Histonet] Placenta returns Message-ID: <000501c7caed$02beb120$ff144246@yourlk4rlmsu> I thought this might be of interest. We had a discussion on this issue several months ago, I believe. http://www.ctv.ca/servlet/ArticleNews/story/CTVNews/20070720/placenta_hospital_070720/20070720?hub=Health Bryan Llewellyn From minniesann <@t> hotmail.com Fri Jul 20 13:45:26 2007 From: minniesann <@t> hotmail.com (Maribel Santiago) Date: Sun Jul 22 06:33:27 2007 Subject: [Histonet] Question? Message-ID: What is going on with histonet? I'm not receiving emails since wednesday afternoon! Minnie _________________________________________________________________ Express yourself instantly with MSN Messenger! Download today it's FREE! http://messenger.msn.click-url.com/go/onm00200471ave/direct/01/ From sjchtascp <@t> yahoo.com Fri Jul 20 18:18:10 2007 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Sun Jul 22 06:40:42 2007 Subject: [Histonet] paraffin heater Message-ID: <20070720231810.88168.qmail@web38203.mail.mud.yahoo.com> I'm looking for the maker of a heater used to remove paraffin from the sides of paraffin tissue cassettes. I used one a a facility I temped at in Milwaukee but didn't get the manufacturer. Thanks, Steve --------------------------------- Be a better Globetrotter. Get better travel answers from someone who knows. Yahoo! Answers - Check it out. From bakevictoria <@t> gmail.com Sat Jul 21 09:29:40 2007 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Sun Jul 22 06:46:20 2007 Subject: [Histonet] Looking for Angie Bailey Message-ID: <4f016b690707210729v4e91e191qab17916456c2a662@mail.gmail.com> Hi I am posting this for a friend if anyone know how I can locate Angie Bailey, please let me know! Thanks in advance. Vikki Baker From machocraig <@t> hotmail.com Sat Jul 21 15:41:39 2007 From: machocraig <@t> hotmail.com (machocraig) Date: Sun Jul 22 06:48:18 2007 Subject: [Histonet] No posts Message-ID: Hi, I am not getting any posts. Is histonet out of commission? Craig From marjoh3 <@t> telus.net Sun Jul 22 08:26:03 2007 From: marjoh3 <@t> telus.net (Marilyn Johnson) Date: Sun Jul 22 08:26:08 2007 Subject: [Histonet] Listeria By Immunohistochemistry Message-ID: <000801c7cc63$da331d40$6401a8c0@VALUED20606295> Hi Histonetters, Thanks to all of you who replied to my request. Greatly appreciated. Marilyn Johnson Food Safety Division Alberta Agriculture Edmonton, AB. Canada From jnocito <@t> satx.rr.com Sun Jul 22 09:10:42 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Sun Jul 22 09:10:47 2007 Subject: [Histonet] Placenta returns References: <000501c7caed$02beb120$ff144246@yourlk4rlmsu> Message-ID: <004401c7cc6a$176b0dc0$d49eae18@yourxhtr8hvc4p> Bryan, two things. First, the placenta was frozen and not placed in formalin. Every hospital I have worked in always placed the placenta in formalin (ok, some might have placed 15 mls in the container, maybe 20). The second thing I noticed was that Nevada has no law about returning specimens to the owner. I've worked at placed were we were being sued by a guy because he wanted his finger back, 18 months ago. Even though we sent him copies of he Texas law and MSDS on 10% NBF, he still found a sleazy lawyer to file suit. Also, I've had experiences where the patient has requested their specimen back because of religious reasons. So here's my point. People who want their specimens back because of religious reasons do so up front. People who want to place their specimen on the coffee table for a conversational piece will request it back as an after thought. Like Billy Bob who wanted his finger back. "Hey Bubba, look what I took home from the hospital". "No you idiot, it's not chicken liver, it's my hemorrhoids". That's my story and I'm sticking to it. JTT ----- Original Message ----- From: "Bryan Llewellyn" To: "Histonet" Sent: Friday, July 20, 2007 11:42 AM Subject: [Histonet] Placenta returns >I thought this might be of interest. We had a discussion on this issue >several months ago, I believe. > > http://www.ctv.ca/servlet/ArticleNews/story/CTVNews/20070720/placenta_hospital_070720/20070720?hub=Health > > Bryan Llewellyn > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Sun Jul 22 09:13:00 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Sun Jul 22 09:13:06 2007 Subject: [Histonet] Question? References: Message-ID: <005701c7cc6a$69ae9fc0$d49eae18@yourxhtr8hvc4p> I think the Histonet went on vacation. Hey, it is summer you know. Except in Texas, it's monsoon season. I picked a real winner of a summer to take off. Okay, if you must know, I AM walking around with the a big L on my forehead. JTT ----- Original Message ----- From: "Maribel Santiago" To: Sent: Friday, July 20, 2007 1:45 PM Subject: [Histonet] Question? What is going on with histonet? I'm not receiving emails since wednesday afternoon! Minnie _________________________________________________________________ Express yourself instantly with MSN Messenger! Download today it's FREE! http://messenger.msn.click-url.com/go/onm00200471ave/direct/01/_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Sun Jul 22 11:13:27 2007 From: liz <@t> premierlab.com (Liz Chlipala) Date: Sun Jul 22 11:13:36 2007 Subject: [Histonet] Unsuccessful Muscle Lipid Staining In-Reply-To: <5053060AF7154CA4A17DE7A1F7241680@PremierLab.local> References: <5053060AF7154CA4A17DE7A1F7241680@PremierLab.local> Message-ID: Son We did this in the past, we post fixed in osmium, it turned out quite = nice. I'll send images in a different e-mail. We fixed in formalin and = then post fixed the muscle in osmium overnight, we were working on mouse = tibialis anterior so we kept the entire muscle intact and processed the = whole muscle and then cut the muscle in half prior to embedding. We = found that the unstained sections turned out the best for demonstration = of the fat. I might have a material and methods some where since I think = they published on it. I'll send that to you with the images. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com =20 Ship to Address: =20 Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu = [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sonny = Duong Sent: Sunday, July 22, 2007 3:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Unsuccessful Muscle Lipid Staining Hi all, I have been trying to stain mouse muscle tissue for intramuscular = triglyceride droplets with Oil Red O (dissolved in triethyl phosphate) = for some time now, and my results have been disastrous. I've tried it = on both fresh frozen and formaldehyde fixed tissues (and cryoprotected) = cryosections, but almost always it seems that the lipid droplets within = the fibers (maybe?) leak out of the cells and conglomerate on the edges = of the tissue and within gaps between the cells. Very few of the = fibers, if any, display any lipid droplet staining and it is always very = weak. The lipid droplets all conglomerate in roughly the same positions = between serial sections as well, which leads me to believe something is = happening during the freezing/fixation process, or the cutting in the = cryosection. Does anyone have any suggestion as to what I could be = doing wrong here (i.e cutting technique, temperature, fixatives)? Also, = does anyone have any experience with using ORO in triethyl phosphate? Thank you all for your time, Sonny Duong University of Virginia Green Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG Free Edition.=20 Version: 7.5.476 / Virus Database: 269.10.12/910 - Release Date: = 7/21/2007 3:52 PM =20 No virus found in this outgoing message. Checked by AVG Free Edition.=20 Version: 7.5.476 / Virus Database: 269.10.12/910 - Release Date: = 7/21/2007 3:52 PM =20 From JWEEMS <@t> sjha.org Sun Jul 22 11:43:11 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Sun Jul 22 11:43:39 2007 Subject: [Histonet] paraffin heater In-Reply-To: <20070720231810.88168.qmail@web38203.mail.mud.yahoo.com> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FF9EE6@sjhaexc02.sjha.org> ThermoFisher Scientific - formerly ThermoElectronFisherScientificThermoShandonShandonLipshawLabVision... Etc! I would look on the web site. I'm not sure if the product number changed after all the merges... Good luck! j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven Coakley Sent: Friday, July 20, 2007 7:18 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] paraffin heater I'm looking for the maker of a heater used to remove paraffin from the sides of paraffin tissue cassettes. I used one a a facility I temped at in Milwaukee but didn't get the manufacturer. Thanks, Steve --------------------------------- Be a better Globetrotter. Get better travel answers from someone who knows. Yahoo! Answers - Check it out. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From Shirley_PHUA <@t> hsa.gov.sg Sun Jul 22 13:01:44 2007 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Sun Jul 22 13:03:29 2007 Subject: [Histonet] Shirley Phua is away on 23/07/2007 (Monday) Message-ID: I will be out of the office from 23-07-2007 to 23-07-2007. I'll be away on 23/07/2007 (Monday). I'll be back on 24/07/2007 (Tuesday). Pathologists: I will process your requests when I return. If urgent, please forward your email to Henry_Kyaw@hsa.gov.sg From denjo <@t> persona.ca Sun Jul 22 14:11:06 2007 From: denjo <@t> persona.ca (Dennis & Mary Jo) Date: Sun Jul 22 14:09:53 2007 Subject: [Histonet] (no subject) Message-ID: <002e01c7cc94$0e4ecd60$39f1a7d8@customer2wtvod> please unsubscribe me Thanks From MVaughan4 <@t> ucok.edu Sun Jul 22 14:19:59 2007 From: MVaughan4 <@t> ucok.edu (MVaughan4@ucok.edu) Date: Sun Jul 22 14:28:04 2007 Subject: [Histonet] Slide subbing vs superfrost plus, pros/cons? Message-ID: Histonetters, Since Maria brought up poly-l-lysine subbing, what are the advantages/disadvantages of subbing slides versus purchasing the superfrost plus slides? I used to sub slides back in the day, but when I have money I will buy the commercial slides. However, recently I have had variable results with tissues falling off during processing, especially protease treatments. I am considering returning to subbing, or at least training undergraduates to do the subbing as coursework. Mel ----------------------------------------- **CONFIDENTIALITY** -This email (including any attachments) may contain confidential, proprietary and privileged information. Any unauthorized disclosure or use of this information is prohibited. From ccross6032 <@t> aol.com Sun Jul 22 16:45:28 2007 From: ccross6032 <@t> aol.com (Cheryl Cross) Date: Sun Jul 22 16:45:41 2007 Subject: [Histonet] IHC for nestin, vesicular inhibitory amino acid transporter, and/or glutamate receptors? Message-ID: Hi histonetters - is anyone doing IHC for any of the above on FFPE rodent brains (or any other mammal for that matter)? If so, any protocol/antibody recommendations? Many thanks! Cheryl Cheryl Cross, DVM, Dipl. ACVP Researcher University Corporation for Atmospheric Research College of Veterinary Medicine University of Tennessee Department of Pathology 2407 River Drive, Room A201 Knoxville, TN 37996-4542 (423) 967-2724 fax: 865-974-5616 ccross@ucar.edu From Joanne.Malinowski <@t> crl.com Mon Jul 23 06:35:30 2007 From: Joanne.Malinowski <@t> crl.com (Malinowski, Joanne O,) Date: Mon Jul 23 06:35:44 2007 Subject: [Histonet] Plastics Bubbles and Troubles Message-ID: <59652984D45D9241B798663CBD2EEA370155C365@wor-crl-exch1.na01.crl.com> Hello All, I have some unanswered questions that I would like to throw out to everyone who works in plastics. * Has anyone figured out yet what the gas is (is it a gas?) that causes the bubbles during polymerization of the MMA3(embedding)? * Can these be de-gassed by hooking up a vacuum to the solution before pouring into the bottle for embedding? * If keeping them in a cooler environment (refrigerator) is a help, is it more helpful to throw in ice-packs to assist? * Is keeping them in a water bath in the refrigerator any help? * Has any real breakthroughs that are realistic, cost effective and efficient been realized? * Has there been an assessment of Exakt vs. Buehler vs. Isomet various equipments been done for the various steps or is the expense too prohibitive to expect? (Who can afford to purchase and evaluate this kind of equipment objectively?) * And, if etching the slides is beneficial to staining, what acid is preferred? (If it is acid.) Is Hydrochloric too harsh? If decolorizing, is hydrochloric used on plastic? So many questions, so few answers! The more we know, the more we need to know... Thank you all in advance for any comments. Joanne Malinowski,HT ASCP Plastics Lab Manager, Medical Devices Division Charles River Laboratories Pathology Associates 15 Worman's Mill Court, Suite I Frederick, Maryland 21701 Phone 301-624-2034 Fax 301-663-8994 Email: joanne.malinowski@us.crl.com From b-frederick <@t> northwestern.edu Mon Jul 23 07:56:34 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon Jul 23 07:56:43 2007 Subject: [Histonet] Problems with the Histonet? In-Reply-To: Message-ID: <002201c7cd28$e5f345e0$d00f7ca5@lurie.northwestern.edu> All, I was having problems last week too. This is the first day I've received messages since last week. And I thought at first it was because IT was playing around with our server. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dolores Townsend Sent: Friday, July 20, 2007 10:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Problems with the Histonet? I have not received any message from the Histonet since Wednesday morning. Anyone else having this problem? Dolores _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vazquezr <@t> ohsu.edu Mon Jul 23 08:42:20 2007 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Mon Jul 23 08:49:58 2007 Subject: [Histonet] Problems with the Histonet? Message-ID: Same here!!! Robyn >>> "Dolores Townsend" 7/20/2007 8:31 AM >>> I have not received any message from the Histonet since Wednesday morning. Anyone else having this problem? Dolores _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pwg1 <@t> CDC.GOV Mon Jul 23 08:42:48 2007 From: pwg1 <@t> CDC.GOV (Greer, Patricia (CDC/CCID/NCZVED)) Date: Mon Jul 23 08:50:00 2007 Subject: [Histonet] vials for autostainer In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB7254@EMAIL.archildrens.org> References: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB7254@EMAIL.archildrens.org> Message-ID: <34BB307EFC9A65429BBB49E330675F7201BA9DCC@LTA3VS003.ees.hhs.gov> Hazel, We order from Sarstedt, a German company that has a distributor in North Carolina. Catalog number is 62-732-012 "tubes, polypropylene, 76 X 20 mm". The last time we ordered they were $92.00 for a case of 500. Below is contact information Sarstedt - US Office P.O. Box 468 Newton, NC (USA) 28658 Phone: 828.465.4000 Fax: 828.465.0718 Toll Free: 800.257.5101 www.sarstedt.com Pat Greer Infectious Disease Pathology Branch Centers for Disease Control and Prevention Mail Stop G-32 Atlanta, GA 30333 404-639-2811 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, July 19, 2007 12:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] vials for autostainer I remember someone on this list saying there were vials for the dako autostainer by another company that were cheaper. Will someone send that information? Thanks so much!! Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org ------------------------------------------------------------------------ ------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From failm <@t> musc.edu Mon Jul 23 08:49:04 2007 From: failm <@t> musc.edu (Mildred Fail) Date: Mon Jul 23 08:51:36 2007 Subject: [Histonet] DAKO autostainer problem Message-ID: Has anyone had problems with the stainer arm stopping in the far right position. Rena Fail From rjbuesa <@t> yahoo.com Mon Jul 23 09:11:28 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jul 23 09:11:33 2007 Subject: [Histonet] DAKO autostainer problem In-Reply-To: Message-ID: <514968.97166.qm@web61218.mail.yahoo.com> It happened to me. Call DAKO to schedule service. Ren? J. Mildred Fail wrote: Has anyone had problems with the stainer arm stopping in the far right position. Rena Fail _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better Heartthrob. Get better relationship answers from someone who knows. Yahoo! Answers - Check it out. From failm <@t> musc.edu Mon Jul 23 09:18:18 2007 From: failm <@t> musc.edu (Mildred Fail) Date: Mon Jul 23 09:18:36 2007 Subject: [Histonet] DAKO autostainer problem Message-ID: Rena Fail >>> Rene J Buesa 07/23/07 10:12 AM >>> It happened to me. Call DAKO to schedule service. Ren? J. Mildred Fail wrote: Has anyone had problems with the stainer arm stopping in the far right position. Rena Fail _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better Heartthrob. Get better relationship answers from someone who knows. Yahoo! Answers - Check it out. From failm <@t> musc.edu Mon Jul 23 09:18:08 2007 From: failm <@t> musc.edu (Mildred Fail) Date: Mon Jul 23 09:21:19 2007 Subject: [Histonet] DAKO autostainer problem Message-ID: were they able to fix it? Rena Fail >>> Rene J Buesa 07/23/07 10:12 AM >>> It happened to me. Call DAKO to schedule service. Ren? J. Mildred Fail wrote: Has anyone had problems with the stainer arm stopping in the far right position. Rena Fail _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better Heartthrob. Get better relationship answers from someone who knows. Yahoo! Answers - Check it out. From gcallis <@t> montana.edu Mon Jul 23 10:04:09 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Jul 23 10:04:14 2007 Subject: [Histonet] Slide subbing vs superfrost plus, pros/cons? In-Reply-To: References: Message-ID: <6.0.0.22.1.20070723085435.01b7c078@gemini.msu.montana.edu> For me, it is a time consideration. Time is money and I do not have the time to order slides, make up reagent, dip, dry etc, etc. For us, the cost of buying Plus charge or PolySine slides is costing my department less than me doing it, and it doesn't take me away from more important tasks when the histo lab does not have extra laboratory staff to do the work. If the researcher does not want to buy the more expensive coated slides, then they have to deal with coatings in their own labs and any attendant problems with poor coating results. Researchers here have to buy all their own slides anyway with their funding. However, some people have to hand sub or coat with silane or poly l lysine when working with large slides or special tissues. With huge decalcified bone sections in the past, we did special gelatin subbing of 4 X 5 inch slides. I think it is important that students and even histotechs should know how to do subbing or plus charge coatings of slides though. it is a good learning experience. At 01:19 PM 7/22/2007, you wrote: >Histonetters, >Since Maria brought up poly-l-lysine subbing, what are the >advantages/disadvantages of subbing slides versus purchasing the superfrost >plus slides? I used to sub slides back in the day, but when I have money I >will buy the commercial slides. However, recently I have had variable >results with tissues falling off during processing, especially protease >treatments. I am considering returning to subbing, or at least training >undergraduates to do the subbing as coursework. >Mel > >----------------------------------------- >**CONFIDENTIALITY** -This email (including any attachments) may >contain confidential, proprietary and privileged information. Any >unauthorized disclosure or use of this information is prohibited. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From AGrobe2555 <@t> aol.com Mon Jul 23 10:14:02 2007 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Mon Jul 23 10:15:02 2007 Subject: [Histonet] need protocol for poly-L-Lysine subbing Message-ID: Maria, Check SIGMA. They sell a pre-diluted poly-L-lysine for slide coating and they also have a protocol/instruction sheet. Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital ************************************** Get a sneak peek of the all-new AOL at http://discover.aol.com/memed/aolcom30tour From TMcNemar <@t> lmhealth.org Mon Jul 23 10:47:22 2007 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Mon Jul 23 10:46:53 2007 Subject: [Histonet] Input on automated microtomes.... Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F4B5@lmhsmail.lmhealth.org> It's budget time and I am in need of at least one automated microtome. I would appreciate any thoughts or recommendations you might have. I would like one that also works manually. I budgeted for a Leica last year but it didn't get approved. This year, I'm asking for two... (maybe I'll get one). I never really thought I'd like an automated microtome but the pain in my shoulder is telling me that I might like it now. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org From relia1 <@t> earthlink.net Mon Jul 23 10:49:39 2007 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Jul 23 10:49:45 2007 Subject: [Histonet] RELIA Histology Careers Bulletin Update 7-23-0-2007 Message-ID: Hi Histonetters, I hope everybody had a great weekend. This is just a quick update on some of my newest positions. All of the positions that I work with are full time permanent positions with premier companies setting the mark to be ?employers of choice? in their respective areas. My clients offer competitive salaries excellent benefits and relocation assistance. New Grads and Experienced Techs are welcome to apply! Here are some of my newest openings: Histo Tech ? Ohio near Columbus Histo Tech ? Berkeley, CA Histo Tech ? VA near Virginia Beach Histo Tech ? MD south of Hagerstown Histo Tech ? Naples, FL Histo Tech ? Orlando, FL 3rd shift (2 positions dermpath lab) I also am recruiting for several great management positions: Histology Manager ? CA Santa Barbara area Histology Manager ? Chicago, IL I also have openings in Texas, Pennsylvania, Washington State, Wisconsin, Massachusetts and California. If you are interested in any of these positions or would like to talk about jobs in other areas please shoot me an e-mail at relia1@earthlink.net or call me toll free at 866-607-3542. (I am available after hours) Also if you know of anyone else who might be interested please feel free to pass this information along to them as well. Have a great day!!! Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net From jqb7 <@t> CDC.GOV Mon Jul 23 10:51:03 2007 From: jqb7 <@t> CDC.GOV (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Mon Jul 23 10:51:18 2007 Subject: [Histonet] Input on automated microtomes.... In-Reply-To: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F4B5@lmhsmail.lmhealth.org> References: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F4B5@lmhsmail.lmhealth.org> Message-ID: <34BB307EFC9A65429BBB49E330675F7202BDBE49@LTA3VS003.ees.hhs.gov> I really like my Microm HM 360. Jeanine Bartlett Infectious Disease Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Monday, July 23, 2007 11:47 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Input on automated microtomes.... It's budget time and I am in need of at least one automated microtome. I would appreciate any thoughts or recommendations you might have. I would like one that also works manually. I budgeted for a Leica last year but it didn't get approved. This year, I'm asking for two... (maybe I'll get one). I never really thought I'd like an automated microtome but the pain in my shoulder is telling me that I might like it now. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Jul 23 10:51:12 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jul 23 10:51:19 2007 Subject: [Histonet] Input on automated microtomes.... In-Reply-To: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F4B5@lmhsmail.lmhealth.org> Message-ID: <787509.35956.qm@web61218.mail.yahoo.com> Keep pushing for the Leica(s). Ren? J. Tom McNemar wrote: It's budget time and I am in need of at least one automated microtome. I would appreciate any thoughts or recommendations you might have. I would like one that also works manually. I budgeted for a Leica last year but it didn't get approved. This year, I'm asking for two... (maybe I'll get one). I never really thought I'd like an automated microtome but the pain in my shoulder is telling me that I might like it now. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Sick sense of humor? Visit Yahoo! TV's Comedy with an Edge to see what's on, when. From sbryant <@t> labpath.com Mon Jul 23 11:39:59 2007 From: sbryant <@t> labpath.com (Susan Bryant) Date: Mon Jul 23 11:43:06 2007 Subject: [Histonet] Input on automated microtomes.... References: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F4B5@lmhsmail.lmhealth.org> Message-ID: <008c01c7cd48$3f3dc2a0$8d01a8c0@labpath.com> Tom, We have 7 Leica automated microtomes and love them!!! S Bryant Knoxville Dermatopathology ----- Original Message ----- From: "Tom McNemar" To: Sent: Monday, July 23, 2007 10:47 AM Subject: [Histonet] Input on automated microtomes.... > It's budget time and I am in need of at least one automated microtome. I would appreciate any thoughts or recommendations you might have. I would like one that also works manually. I budgeted for a Leica last year but it didn't get approved. This year, I'm asking for two... (maybe I'll get one). > > I never really thought I'd like an automated microtome but the pain in my shoulder is telling me that I might like it now. > > Tom McNemar, HT(ASCP) > Histology Co-ordinator > Licking Memorial Health Systems > (740) 348-4163 > (740) 348-4166 > tmcnemar@lmhealth.org > www.LMHealth.org > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ---------------------------------------------------------------------------- ---- From jcline <@t> wchsys.org Mon Jul 23 11:53:24 2007 From: jcline <@t> wchsys.org (Joyce Cline) Date: Mon Jul 23 11:53:35 2007 Subject: [Histonet] paraffin heater/steve In-Reply-To: <20070720231810.88168.qmail@web38203.mail.mud.yahoo.com> Message-ID: <001001c7cd49$fc1cefd0$1d2a14ac@wchsys.org> It's called a ParaTrimmer and it is from ThermoShandon/Fisher whatever !!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven Coakley Sent: Friday, July 20, 2007 7:18 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] paraffin heater I'm looking for the maker of a heater used to remove paraffin from the sides of paraffin tissue cassettes. I used one a a facility I temped at in Milwaukee but didn't get the manufacturer. Thanks, Steve --------------------------------- Be a better Globetrotter. Get better travel answers from someone who knows. Yahoo! Answers - Check it out. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From indytreegers <@t> sbcglobal.net Mon Jul 23 12:21:57 2007 From: indytreegers <@t> sbcglobal.net (Lyn Treeger) Date: Mon Jul 23 12:22:05 2007 Subject: [Histonet] Blue background on H & E slides... In-Reply-To: <001001c7cd49$fc1cefd0$1d2a14ac@wchsys.org> Message-ID: <691082.19131.qm@web81915.mail.mud.yahoo.com> Hi Everyone, A while back, I saw some tips posted for removing a blue background from H & E slides. One tip was to rinse in warm H20. We tried that, and it seemed to be working, but recently, we've starting seeing the bacground tint again. Are there any other things that we could try? Thanks so much, Lyn Lyn Treeger, B.S. HT(ASCP) From drkwolfe <@t> telus.net Mon Jul 23 12:26:51 2007 From: drkwolfe <@t> telus.net (Joseph Kapler) Date: Mon Jul 23 12:26:55 2007 Subject: [Histonet] Blue background on H & E slides... In-Reply-To: <691082.19131.qm@web81915.mail.mud.yahoo.com> Message-ID: A quick dip in an acid bath helps. 1 quick dip, no longer than a second or two should clear the haze Joe Kapler -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Lyn Treeger Sent: Monday, July 23, 2007 11:22 To: Histo Net Subject: [Histonet] Blue background on H & E slides... Hi Everyone, A while back, I saw some tips posted for removing a blue background from H & E slides. One tip was to rinse in warm H20. We tried that, and it seemed to be working, but recently, we've starting seeing the bacground tint again. Are there any other things that we could try? Thanks so much, Lyn Lyn Treeger, B.S. HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hhawkins <@t> utmb.edu Mon Jul 23 12:36:35 2007 From: hhawkins <@t> utmb.edu (Hawkins, Hal K.) Date: Mon Jul 23 12:36:40 2007 Subject: [Histonet] galactosidase control Message-ID: We are using immunohistochemistry to detect expression of beta-galactosidase from a transgene applied to healing wounds. We would really like to have a good positive control. Does anyone have a block they could spare for this purpose that shows selective expression of galactosidase, or know of a commercial source? Thanks, Hal Hawkins, UTMB Galveston From JPCOLEMA <@t> sentara.com Mon Jul 23 12:49:23 2007 From: JPCOLEMA <@t> sentara.com (JOHN P COLEMAN) Date: Mon Jul 23 12:49:49 2007 Subject: [Histonet] Microtomes/ Cryostats In-Reply-To: <20070723165924.7A80E1B36D6@spamnet01.sentara.com> References: <20070723165924.7A80E1B36D6@spamnet01.sentara.com> Message-ID: We tried the Leica and Surgipath Motorized microtomes and the leica instrument is great. The Surgipath instrument seemed like a duct tape and chewing gum retrofit, and the mechanism was clumsy. I've seen that the quality at all levels of microtome leica sells is great. within the last year we've gotten 6 including one motorized and all the techs like them. Does anyone offer a tabletop cryostat? Hacker Bright used to sell one that was awful. I need one that works. John P.J. Coleman HT(ASCP)QIHC Clinical Specialist Anatomic Pathology Sentara Laboratory Services Cell/Voicemail(757)335-2159 pager: (757)456-6695 From lblazek <@t> digestivespecialists.com Mon Jul 23 13:09:38 2007 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Mon Jul 23 13:06:27 2007 Subject: [Histonet] Input on automated microtomes.... In-Reply-To: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F4B5@lmhsmail.lmhealth.org> References: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F4B5@lmhsmail.lmhealth.org> Message-ID: <1F937FB30BDB7C4A9F39F83FEA8D379F3E4B55@bruexchange1.digestivespecialists.com> I love my Microm! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Monday, July 23, 2007 11:47 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Input on automated microtomes.... It's budget time and I am in need of at least one automated microtome. I would appreciate any thoughts or recommendations you might have. I would like one that also works manually. I budgeted for a Leica last year but it didn't get approved. This year, I'm asking for two... (maybe I'll get one). I never really thought I'd like an automated microtome but the pain in my shoulder is telling me that I might like it now. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Malcolm.McCallum <@t> tamut.edu Mon Jul 23 13:43:01 2007 From: Malcolm.McCallum <@t> tamut.edu (Malcolm McCallum) Date: Mon Jul 23 13:46:43 2007 Subject: [Histonet] skeletochronology question References: Message-ID: hello histoland, A student and I recently did some skeletochronology on some salamanders. We found a segment of animals with no growth lines (< 1 yr) and then animals up to 11 yrs old. There was no relationship between age and body size. Still, the growth rings were present in the bone. Differences in growth between years was evident, but clearly they were not growing! This seems to suggest that the growth rings are not useful for modeling growth in this species, or that they reach maximum growth within their first year. ANy thoughts??? Malcolm L. McCallum Assistant Professor Department of Biological Sciences Texas A&M University Texarkana 2600 Robison Rd. Texarkana, TX 75501 O: 1-903-223-3134 H: 1-903-791-3843 Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html VISIT HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org A New Journal Published in Partnership with Partners in Amphibian and Reptile Conservation and the World Congress of Herpetology. Summer Teaching Schedule & Office Hours: Monday-Thursday: Statistics (Math 453) 4-5:50 pm Office Hours: 3-4 pm "Set high standards for yourself, but don't judge others by those standards," - Gary Emmert (University of Memphis) ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of ADESUPO ADESUYI Sent: Sun 7/1/2007 12:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pipettes Calibration Hi, Pls we are trying to calibrate our pipettes, so i will appreciate it, if you guys could suggest some ideas to me, on how to go about doing this. You guys are the best. Ade. _________________________________________________________________ Play free games, earn tickets, get cool prizes! Join Live Search Club. http://club.live.com/home.aspx?icid=CLUB_wlmailtextlink_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Mon Jul 23 14:02:22 2007 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Mon Jul 23 14:02:47 2007 Subject: [Histonet] re: IHC for nestin, vesicular inhibitory amino acid Message-ID: <000e01c7cd5c$00372dd0$4101a8c0@carlba65530bda> http://www.immunoportal.com/index.php If you care to, please have a look in the IHC Gallery on this site. I hope that they are useful. Join and post any further queries there or, just post here........ ....if any pics are of interest to you. Carl From rjbuesa <@t> yahoo.com Mon Jul 23 14:23:28 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jul 23 14:23:32 2007 Subject: [Histonet] Blue background on H & E slides... In-Reply-To: <691082.19131.qm@web81915.mail.mud.yahoo.com> Message-ID: <262462.78269.qm@web61222.mail.yahoo.com> Take the slides and dip them (just IN and OUT) of the same solution you use to differentiate the hematoxylin. Ren? J. Lyn Treeger wrote: Hi Everyone, A while back, I saw some tips posted for removing a blue background from H & E slides. One tip was to rinse in warm H20. We tried that, and it seemed to be working, but recently, we've starting seeing the bacground tint again. Are there any other things that we could try? Thanks so much, Lyn Lyn Treeger, B.S. HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Choose the right car based on your needs. Check out Yahoo! Autos new Car Finder tool. From oshel1pe <@t> cmich.edu Mon Jul 23 14:58:00 2007 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Mon Jul 23 14:58:17 2007 Subject: [Histonet] skeletochronology question In-Reply-To: References: Message-ID: Bone acts as a calcium reserve for metabolism, so maybe calcium resorption then redeposition, depending on time of year? E.g., times of low food, the critters use bone calcium to meet metabolic needs. Any correlations between sex and "age", distinctness of the bands, or width of the bands? Females would have greater need for calcium during ovipositing, so should show greater resorption/redeposition effects than males. Do the bands correlate with seasons as opposed to calendar years? Phil >hello histoland, > >A student and I recently did some skeletochronology on some >salamanders. We found a segment of animals with no growth lines (< >1 yr) and then animals up to 11 yrs old. There was no relationship >between age and body size. Still, the growth rings were present in >the bone. Differences in growth between years was evident, but >clearly they were not growing! This seems to suggest that the >growth rings are not useful for modeling growth in this species, or >that they reach maximum growth within their first year. > >ANy thoughts??? > > >Malcolm L. McCallum >Assistant Professor >Department of Biological Sciences >Texas A&M University Texarkana >2600 Robison Rd. >Texarkana, TX 75501 >O: 1-903-223-3134 >H: 1-903-791-3843 >Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html >VISIT HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org > >A New Journal Published in Partnership with Partners in Amphibian >and Reptile Conservation >and the World Congress of Herpetology. >Summer Teaching Schedule & Office Hours: >Monday-Thursday: > Statistics (Math 453) 4-5:50 pm > Office Hours: 3-4 pm > >"Set high standards for yourself, but don't judge others by those standards," >- Gary Emmert (University of Memphis) -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 From asachau <@t> titanmed.com Mon Jul 23 15:04:40 2007 From: asachau <@t> titanmed.com (April Sachau) Date: Mon Jul 23 15:06:08 2007 Subject: [Histonet] skeletochronology question In-Reply-To: Message-ID: <7E3ACD48BA6E26408F3188FBF08693F7AA166C@titansbs1.corp.titanmed.com> Hello Histonetters! I am in need of a NY licensed Histologist for two months. It is in upstate NY. Let me know if you know of anyone that might be interested. Thanks! April Sachau Titan Medical Group Staff Supervisor Phone (866) 332-9600 Ext. 1023 Fax (402) 332-5181 asachau@titanmed.com see us on the web at www.titanmed.com From koellingr <@t> comcast.net Mon Jul 23 16:04:27 2007 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Mon Jul 23 16:04:34 2007 Subject: [Histonet] galactosidase control Message-ID: <072320072104.8417.46A517DB0001D284000020E122007504389D09020704040A0105@comcast.net> Hal, Since you are working with transgenes in wound healing, I'm assuming this is animal work. What I used to use for a great control was to get a mouse from (I think Jackson labs have them-but certainly one of the major mouse vendors) that is an FVB mouse with a lacZ transgene that is under control of a Tie2 promotor. Tie2 is endothelial, so can look for beta-gal expression anywhere there is endothelial cells. So essentially 1 mouse gives you all the controls, many tissues are fantastic, you could ever need and the staining on endothelium is specific and exquisite. Ray Koelling PhenoPath Laboratories Seattle, WA -------------- Original message -------------- From: "Hawkins, Hal K." > > > We are using immunohistochemistry to detect expression of > beta-galactosidase from a transgene applied to healing wounds. We would > really like to have a good positive control. Does anyone have a block > they could spare for this purpose that shows selective expression of > galactosidase, or know of a commercial source? > > > > Thanks, > > > > Hal Hawkins, UTMB Galveston > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amian <@t> thermage.com Mon Jul 23 16:12:41 2007 From: amian <@t> thermage.com (Arshia Mian) Date: Mon Jul 23 16:12:57 2007 Subject: [Histonet] re: Saturated lithium carbonate Message-ID: <74D1E69EF46F3445B710962F3E10D1AC02ECBC55@thmail.thermage.com> Hello, Has anyone had problems getting lithium carbonate to dissolve in H2O to make a saturated aqueous soln? I have tried 2 different concentrations of lithium carbonate to go into solution. 1. 1.54g in 100ml H2O 2. 1g in 100ml H2O I am trying to make this solution for the Herovici stain, but with little success. I have called the manufacturer of the lithium carbonate and they said that the solution is soluble at 20degC in H2O at a conc. of 1.3g in 100ml H2O. Any help would be appreciated. Thanks. Arshia From PMonfils <@t> Lifespan.org Mon Jul 23 16:37:41 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Mon Jul 23 16:37:48 2007 Subject: [Histonet] Ripples in Arterial sections In-Reply-To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E273CB4@LSRIEXCH1.lsmaster.lifespan.org> This is common when cutting sections of artery. Actually the section isn't contracting after being cut. Rather, just the opposite. Virtually all tissues undergo substantial shrinkage during dehydration. The cut sections then rehydrate when floated on the water bath, and expand to approximately the original size of the tissue sample. Some tissues rehydrate quickly, others more slowly, and as a result, sections of some organs which are composed of different kinds of tissue may not rehydrate at a uniform rate. Muscle tends to rehydrate slowly, which is why arterial sections, either alone or within sections of various organs, develop these "waves". The connective tissues, collagen, and other components around the muscle fibers expand rapidly on the water bath, pulling on the muscle tissue which is more resistant to expansion. There are a few options that will help eliminate this artifact. First - patience! If your water bath is sufficiently warm, such "waves" in the tissue will usually flatten considerably if not completely, if you wait long enough. You may have to let the sections float a couple of minutes or more. Second, if they don't flatten sufficiently after a couple of minutes, increase the temperature of your water bath, close to the melting point of the paraffin you are using. That will cause the sections to spread more quickly. And/or, third, use a "slide warmer". If you are not familiar with that piece of equipment, it's basically a low temperature hotplate (maximum temp usually about 80 degrees C), with a larger surface area than a standard hotplate. You cut your sections, drain them briefly, then lay the slides flat on the slide warmer, while there is still some moisture between the section and the slide.. At 80 degrees the wax in the sections will melt immediately, and the tissue will quickly spread and adhere to the slide. Leave them a couple of minutes or more, until all the water has evaporated from the slide. 80 degrees may be too warm for more delicate tissues, which may overspread, but relatively tough tissues like artery will do well at that temperature. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of JR R > Sent: Wednesday, July 18, 2007 6:55 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Ripples in Arterial sections > > > I am cutting 5 micron sections from formalin fixed, paraffin embedded > segments of rabbit abdominal aorta. > > I float my ribbons in a warm water bath then pick them up with a > Superfrost Plus slide. > > When I check my sections uner the microscope, the sections have a > wavy, or rippled appearance--It is not really "wrinkles, in the sense > of the paraffin folding and sticking to itself--it is as if the > arterial segment is contracting after being cut. It looks like a > circular roadway that dips down then up, then down, then up... > > Has anyone ever seen this artifact with arterial sections? Any > ideas abiut why uit happens and what I could do to prevent it? > > Thanks > > > Jerry L. Ricks > > Research Scientist > > U.W. Medicine at South Lake Union > > 815 Mercer Street > > Seattle, WA 98109 > > (206)-685-7190 > _________________________________________________________________ > > [1]See what youre getting intobefore you go there > > References > > 1. http://g.msn.com/8HMAENUS/2734??PS=47575 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From jkiernan <@t> uwo.ca Mon Jul 23 16:53:19 2007 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Mon Jul 23 16:53:28 2007 Subject: [Histonet] re: Saturated lithium carbonate In-Reply-To: <74D1E69EF46F3445B710962F3E10D1AC02ECBC55@thmail.thermage.com> References: <74D1E69EF46F3445B710962F3E10D1AC02ECBC55@thmail.thermage.com> Message-ID: The solubility of lithium carbonate in water is 1.3g per 100 ml, according to Lange's Handbook of Chemistry. Add any quantity greater than this to a bottle containing 100 ml of water, shake and wait for the undissolved material to settle on the bottom. As long as some undissolved lithium carbonate is present, the solution is saturated. When I make this solution, I put in a large excess of lithium carbonate, and top up with water when the bottle is getting low. As far as I know, the only reason for using lithium carbonate rather than the cheaper sodium carbonate is that Li2CO3 is less soluble than Na2CO3 (solubility = 7.1g per 100 ml.). It's always easy to work with saturated solutions because weighing is not needed. Saturated Li2CO3 is 0.18M whereas saturated Na2CO3 is 0.67M and therefore much more strongly alkaline. A 1.86% solution of anhydrous sodium carbonate is 0.18M and should behave the same as a saturated lithium carbonate solution for differentiating anionic dyes etc. John Kiernan Anatomy, UWO London, Canada --- ----- Original Message ----- From: Arshia Mian Date: Monday, July 23, 2007 17:14 Subject: [Histonet] re: Saturated lithium carbonate To: histonet@lists.utsouthwestern.edu > Hello, > > Has anyone had problems getting lithium carbonate to dissolve in > H2O to > make a saturated aqueous soln? > > > > I have tried 2 different concentrations of lithium carbonate to > go into > solution. > > 1. 1.54g in 100ml H2O > > 2. 1g in 100ml H2O > > > > I am trying to make this solution for the Herovici stain, but with > little success. > > I have called the manufacturer of the lithium carbonate and they said > that the solution is soluble at 20degC in H2O at a conc. of 1.3g in > 100ml H2O. > > > > Any help would be appreciated. > > Thanks. > > Arshia > > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From barbaraaalbert <@t> yahoo.com Mon Jul 23 18:33:25 2007 From: barbaraaalbert <@t> yahoo.com (Barbara Albert) Date: Mon Jul 23 18:33:30 2007 Subject: [Histonet] forced air section dryer Message-ID: <79452.65516.qm@web63707.mail.re1.yahoo.com> Hi all, Do any of you use a forced air section dryer on your slides before H&E? We're looking at switching from an oven when we move in a few months and would appreciate any feedback you can offer. We're looking at the Shandon High Capacity model. Thanks, Barbara Albert Lead Histotechnologist UCSF Medical Center San Francisco, CA --------------------------------- Park yourself in front of a world of choices in alternative vehicles. Visit the Yahoo! Auto Green Center. From mauger <@t> email.chop.edu Tue Jul 24 09:18:40 2007 From: mauger <@t> email.chop.edu (Joanne Mauger) Date: Tue Jul 24 09:19:18 2007 Subject: [Histonet] vials for autostainer Message-ID: Hazel, We order reagent vials from Labvision through Fisher#S20002. You get 100-15 ml. vials for $40. They are exactly the same as the ones from Dako. Jo From NSEARCY <@t> swmail.sw.org Tue Jul 24 11:10:10 2007 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Tue Jul 24 11:10:29 2007 Subject: [Histonet] ISH- Kappa / Lambda Message-ID: Am seeking information from the group regarding the use of ISH for Kappa and Lambda instead of immunohistochemistry. Anyone doing this methodology? Thanks From gcallis <@t> montana.edu Tue Jul 24 11:23:11 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Jul 24 11:22:48 2007 Subject: [Histonet] re: Saturated lithium carbonate In-Reply-To: <74D1E69EF46F3445B710962F3E10D1AC02ECBC55@thmail.thermage.c om> References: <74D1E69EF46F3445B710962F3E10D1AC02ECBC55@thmail.thermage.com> Message-ID: <6.0.0.22.1.20070724100924.01b5bc60@gemini.msu.montana.edu> A saturated solution means you should have MORE lithium carbonate than can go into solution and you will see some of the lithium carbonate salt sitting on the bottom of the container. Only so much salt can be dissolved and with a saturated solution, you exceed that amount that is in solution. Merck Index: 1 gm dissolves in 78 ml of cold water. If you add MORE salt than 1 gm for that amount of water, it will be a saturated solution where the LiCO3 cannot go totally into solution. To use a saturated solution, then decant (pour off) some of the liquid from the top of the salt sitting on the bottom of the bottle but be careful to not agitate the saturated solution when you do this. At 03:12 PM 7/23/2007, you wrote: >Hello, > >Has anyone had problems getting lithium carbonate to dissolve in H2O to >make a saturated aqueous soln? > >I have tried 2 different concentrations of lithium carbonate to go >intosolution. > >1. 1.54g in 100ml H2O >2. 1g in 100ml H2O >I am trying to make this solution for the Herovici stain, but with >little success. >I have called the manufacturer of the lithium carbonate and they said >that the solution is soluble at 20degC in H2O at a conc. of 1.3g in >100ml H2O. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From cgfields <@t> lexhealth.org Tue Jul 24 12:02:09 2007 From: cgfields <@t> lexhealth.org (Carole Fields) Date: Tue Jul 24 12:02:26 2007 Subject: [Histonet] Unsubscribe Message-ID: Thanks Carole Fields, HT,ASCP Pathology Supervisor Lexington Medical Center 2720 Sunset Blvd. W. Columbia, SC 29169 (803) 936-8214 From failm <@t> musc.edu Tue Jul 24 12:28:32 2007 From: failm <@t> musc.edu (Mildred Fail) Date: Tue Jul 24 12:29:11 2007 Subject: [Histonet] ISH- Kappa / Lambda Message-ID: We are in the process of testing a Benchmark, one of the products weare using on the machine is the ISH for Kappa and Lambda, The results are very imppressive. More cells are staining. There is NO background staining. It is very clean. i chose bone marrow cases that had been stained IHC for Kappa and Lambda to test with the ISH for Kappa and Lambda. even though our controls are clearly cleaner with only a few more cells staining with ISH. The pt cases run in the last 4 weeks clearly had more cells staining with ISH. Our controls are from bxs on previous pts chosen by the pathologist. I know after reviewing these slides I am uncomfortable with the results using IHC Rena Fail >>> "Nita Searcy" 07/24/07 12:12 PM >>> Am seeking information from the group regarding the use of ISH for Kappa and Lambda instead of immunohistochemistry. Anyone doing this methodology? Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From crochieresteve <@t> aol.com Tue Jul 24 12:31:38 2007 From: crochieresteve <@t> aol.com (crochieresteve@aol.com) Date: Tue Jul 24 12:31:54 2007 Subject: [Histonet] Stainer for sale Message-ID: <8C99C2F9FE03980-1614-D3ED@FWM-D37.sysops.aol.com> Hello, I have a "gently used" Thermo Gemini Varistainer for sale. If interested please contact: Steve Crochiere Histology Supervisor LifePath Partners, LLC / New England Pathology Assoc. 299 Carew St. Springfield, MA 01104 413-748-9541 ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From rbruggeman <@t> psu.edu Tue Jul 24 12:58:57 2007 From: rbruggeman <@t> psu.edu (Richard Bruggeman) Date: Tue Jul 24 12:59:32 2007 Subject: [Histonet] Dako's Seymour Slide Labels Message-ID: <46A605A1.48B8.00C6.0@psu.edu> I tried to place an order with Dako for Seymour slide labels and printer supplies and Dako claims to be out of this product and can not provide me with an expected ship date or alternate vendor. Has anyone found an alternate source for the printer supplies and slide labels to use with the Dako Seymour slide labeler. Thanks. Trey Bruggeman Milton S. Hershey Medical Center Penn State College of Medicine Department of Pathology 500 University Drive Hershey, PA 17033 (717) 531-1044 From christiegowan <@t> msn.com Tue Jul 24 14:03:25 2007 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Tue Jul 24 14:03:30 2007 Subject: [Histonet] Leica Autostainer Message-ID: Hi Folks, I was hoping someone out in histoland would have some used or new wash station containers they would like to part with. Ours are corroded and the water is not flowing well. If someone has an old or not in use machine and they wouldn't mind parting with some containers, I would be happy to take them off their hands. I'm just looking to save some money on an old machine. Thanks! Christie Gowan From SRazz <@t> brch.com Tue Jul 24 14:17:09 2007 From: SRazz <@t> brch.com (Shirlene Razz) Date: Tue Jul 24 14:17:27 2007 Subject: [Histonet] p120 catenin antibody, clone 98 Message-ID: Hi, I'd like to know if anyone out there is using the antibody p120 catenin, clone 98 by paraffin section. Is useful in assisting diagnosing lobular vs ductal breast neoplasms. Used in conjuction with ecadherin independently. I'd like to get it working for the Dr's here. Thank for your help in advance. Shirlene Razz Boca Raton Community Hospital 561-955-4773 e-mail: srazz@brch.com From sjchtascp <@t> yahoo.com Tue Jul 24 15:12:08 2007 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Tue Jul 24 15:12:11 2007 Subject: [Histonet] microtomy instruction resources Message-ID: <863698.50760.qm@web38201.mail.mud.yahoo.com> Can anyone suggest where I can get a good visual instruction resource for microtomy. The lab I'm contracting in is made up of untrained HT's that really need the fine tuning. We do have allot of good text books on microtomy. Thanks --------------------------------- Get the free Yahoo! toolbar and rest assured with the added security of spyware protection. From pruegg <@t> ihctech.net Tue Jul 24 15:15:22 2007 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Jul 24 15:15:29 2007 Subject: [Histonet] neutralizing formalin with formalex Message-ID: <008401c7ce2f$5e183ce0$6801a8c0@Patsy> If anyone uses formalex to neutralize formalin could you give me the ratio of formalex to waste formalin to polymerize the aldehyde? Thank you Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC 12635 Montview Blvd. Ste.215 Aurora, Colorado 80045 Phone: 720-859-4060 Fax: 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org From marysia33 <@t> hotmail.com Tue Jul 24 15:20:06 2007 From: marysia33 <@t> hotmail.com (Mary Mullinax) Date: Tue Jul 24 15:20:13 2007 Subject: [Histonet] Tampa Florida job opening Message-ID: I am placing this ad for my employer. This is a great place to work. Fast paced, great people! We only want professionals that care to do GREAT work. Every slide counts, as we all know each slide is attached to a person (patient). AmeriPath is currently looking for an HT or HTL. Full time position. Email resume to ACFStaffing@ameripath.com or fax to 407.587.4213 The ad is also placed on Monster.com for more info. From HoustonR <@t> chi.osu.edu Tue Jul 24 15:20:05 2007 From: HoustonR <@t> chi.osu.edu (Houston, Ronald) Date: Tue Jul 24 15:20:49 2007 Subject: [Histonet] microtomy instruction resources In-Reply-To: <863698.50760.qm@web38201.mail.mud.yahoo.com> Message-ID: <979FF5962E234F45B06CF0DB7C1AABB210FA8432@chi2k3ms01.columbuschildrens.net> There's nothing that can compare to one-on-one instruction from a competent microtomist Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Columbus Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 houstonr@chi.osu.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven Coakley Sent: Tuesday, July 24, 2007 4:12 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] microtomy instruction resources Can anyone suggest where I can get a good visual instruction resource for microtomy. The lab I'm contracting in is made up of untrained HT's that really need the fine tuning. We do have allot of good text books on microtomy. Thanks --------------------------------- Get the free Yahoo! toolbar and rest assured with the added security of spyware protection. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: This e-mail message, from Children's Hospital, Columbus, Ohio, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes pursuant to Children's Hospital's confidentiality policies. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From Wanda.Smith <@t> HCAhealthcare.com Tue Jul 24 15:37:59 2007 From: Wanda.Smith <@t> HCAhealthcare.com (Smith Wanda) Date: Tue Jul 24 15:38:12 2007 Subject: [Histonet] Input on automated microtomes.... In-Reply-To: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F4B5@lmhsmail.lmhealth.org> References: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F4B5@lmhsmail.lmhealth.org> Message-ID: <817C2761C5A1394180709AEEDB775B7E02FB48E3@NASEV03.hca.corpad.net> Tom, I have only used the Microm HM 355S, and loved it. At one point I could cut faster on it than a manual microtome. Easy to use and the ones I have had were little to no maintenance. Wanda Wanda G. Smith, HTL(ASCP)HT Trident Medical Center Pathology Supervisor ph: 843-847-4586 fx: 843-847-4296 email: wanda.smith@hcahealthcare.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Monday, July 23, 2007 11:47 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Input on automated microtomes.... It's budget time and I am in need of at least one automated microtome. I would appreciate any thoughts or recommendations you might have. I would like one that also works manually. I budgeted for a Leica last year but it didn't get approved. This year, I'm asking for two... (maybe I'll get one). I never really thought I'd like an automated microtome but the pain in my shoulder is telling me that I might like it now. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sqd3f <@t> cms.mail.virginia.edu Tue Jul 24 15:44:37 2007 From: sqd3f <@t> cms.mail.virginia.edu (Sonny Duong) Date: Tue Jul 24 15:44:42 2007 Subject: [Histonet] OCT tissuetek Message-ID: <4D66F259C30FBE3B842431BE@[192.168.1.136]> Hi all, How long can samples be kept in OCT embedding media in a -80 freezer before they go bad? Forever? A day? I've been trying to stain for intramuscular triglyceride droplets with Oil Red O and it seems that after a couple of days the tissues embedded in OCT stain weaker and eventually don't stain at all. Any help would be appreciated! Sonny Duong Green Lab University of Virginia From rjbuesa <@t> yahoo.com Tue Jul 24 16:24:41 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jul 24 16:24:44 2007 Subject: [Histonet] OCT tissuetek In-Reply-To: <4D66F259C30FBE3B842431BE@[192.168.1.136]> Message-ID: <809336.51470.qm@web61223.mail.yahoo.com> I used to have them for years although they were not intended to save fat. It was a tumor bank used from time to time in research, always successfully. As long as they don't thaw (electrical failure causing the freezer to thaw) the samples can be kept for years. Ren? J. Sonny Duong wrote: Hi all, How long can samples be kept in OCT embedding media in a -80 freezer before they go bad? Forever? A day? I've been trying to stain for intramuscular triglyceride droplets with Oil Red O and it seems that after a couple of days the tissues embedded in OCT stain weaker and eventually don't stain at all. Any help would be appreciated! Sonny Duong Green Lab University of Virginia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get the Yahoo! toolbar and be alerted to new email wherever you're surfing. From mcauliff <@t> umdnj.edu Tue Jul 24 16:35:15 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Jul 24 16:35:50 2007 Subject: [Histonet] microtomy instruction resources In-Reply-To: <863698.50760.qm@web38201.mail.mud.yahoo.com> References: <863698.50760.qm@web38201.mail.mud.yahoo.com> Message-ID: <46A67093.80504@umdnj.edu> Get a digital camera, either still or video, and make your own instructional materials. Geoff Steven Coakley wrote: > Can anyone suggest where I can get a good visual instruction resource for microtomy. > The lab I'm contracting in is made up of untrained HT's that really need the fine tuning. > We do have allot of good text books on microtomy. > > Thanks > > > --------------------------------- > Get the free Yahoo! toolbar and rest assured with the added security of spyware protection. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From jstaruk <@t> masshistology.com Tue Jul 24 17:12:12 2007 From: jstaruk <@t> masshistology.com (Jim Staruk) Date: Tue Jul 24 17:08:51 2007 Subject: [Histonet] neutralizing formalin with formalex In-Reply-To: <008401c7ce2f$5e183ce0$6801a8c0@Patsy> References: <008401c7ce2f$5e183ce0$6801a8c0@Patsy> Message-ID: <000f01c7ce3f$b020f580$4001a8c0@FrontOffice> Hi Patsy, We add 250 ml of Formalex to a gallon of 10% formalin. Jim ______________________ Jim Staruk Mass Histology Service www.masshistology.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Tuesday, July 24, 2007 4:15 PM To: 'Histonet' Subject: [Histonet] neutralizing formalin with formalex If anyone uses formalex to neutralize formalin could you give me the ratio of formalex to waste formalin to polymerize the aldehyde? Thank you Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC 12635 Montview Blvd. Ste.215 Aurora, Colorado 80045 Phone: 720-859-4060 Fax: 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Tue Jul 24 18:06:36 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Jul 24 18:06:40 2007 Subject: [Histonet] ISH- Kappa / Lambda References: Message-ID: <000701c7ce47$49453260$d49eae18@yourxhtr8hvc4p> Nita, mon ami, pisano. The lab uses Ventana's XT with their probes and detection system. If you don't have that, I can't help you. JTT ----- Original Message ----- From: "Nita Searcy" To: Cc: "Juden Francois" Sent: Tuesday, July 24, 2007 11:10 AM Subject: [Histonet] ISH- Kappa / Lambda > Am seeking information from the group regarding the use of ISH for Kappa > and Lambda instead of immunohistochemistry. Anyone doing this > methodology? > > Thanks > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mickie25 <@t> netzero.net Tue Jul 24 19:33:26 2007 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Tue Jul 24 19:34:04 2007 Subject: [Histonet] OCT tissuetek In-Reply-To: <4D66F259C30FBE3B842431BE@[192.168.1.136]> References: <4D66F259C30FBE3B842431BE@[192.168.1.136]> Message-ID: Dear Sonny, We performed IHC on frozen LN for lymphoma immunophenotyping routinely at Sacred Heart Medical Center. We found the samples stained consistently for up to 6 months is not thawed and stored at -70 degrees C. We saved the tissue right on the chuck. I don't have any experience with IM trygliceride droplets. Good Luck! Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sonny Duong Sent: Tuesday, July 24, 2007 1:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] OCT tissuetek Hi all, How long can samples be kept in OCT embedding media in a -80 freezer before they go bad? Forever? A day? I've been trying to stain for intramuscular triglyceride droplets with Oil Red O and it seems that after a couple of days the tissues embedded in OCT stain weaker and eventually don't stain at all. Any help would be appreciated! Sonny Duong Green Lab University of Virginia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rshooki_99 <@t> yahoo.com Wed Jul 25 06:25:15 2007 From: rshooki_99 <@t> yahoo.com (richard shook) Date: Wed Jul 25 06:25:27 2007 Subject: [Histonet] unsiscribe Message-ID: <804445.67829.qm@web36107.mail.mud.yahoo.com> please unsiscribe me please thank you rshooki_99@yahoo.com ____________________________________________________________________________________Ready for the edge of your seat? Check out tonight's top picks on Yahoo! TV. http://tv.yahoo.com/ From stamptrain <@t> yahoo.com Wed Jul 25 06:44:18 2007 From: stamptrain <@t> yahoo.com (Roger Moretz) Date: Wed Jul 25 06:44:23 2007 Subject: [Histonet] unsiscribe In-Reply-To: <804445.67829.qm@web36107.mail.mud.yahoo.com> Message-ID: <374302.62871.qm@web55802.mail.re3.yahoo.com> Well, not knowing what that word is, I can't help. Read your original sign-on message for how to ... whatever. Roger Moretz, Ph.D. (ret.) --- richard shook wrote: > please unsiscribe me please > > > thank you > rshooki_99@yahoo.com > > > > ____________________________________________________________________________________Ready > for the edge of your seat? > Check out tonight's top picks on Yahoo! TV. > http://tv.yahoo.com/ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ____________________________________________________________________________________ Yahoo! oneSearch: Finally, mobile search that gives answers, not web links. http://mobile.yahoo.com/mobileweb/onesearch?refer=1ONXIC From RCazares <@t> schosp.org Wed Jul 25 09:49:03 2007 From: RCazares <@t> schosp.org (Cazares, Ruth) Date: Wed Jul 25 09:49:20 2007 Subject: [Histonet] Use of molecular sieve beads Message-ID: <913FAC2B773C19488E26AE6572180FA509BF0241@exch01.schosp.org> Hello histonetters, I have just read an article about molecular sieve beads being used in xylene to extract the water, and therefore extending the life of the xylene indefinitely. This article was given to me by a cytologist. She came across it on the cytology equivalent to the Histonet. So my question is, has anybody in histology used this method to keep xylene moisture free? And if so, do it really extend the life of the xylene indefinitely? Any and all information is greatly appreciated! Ruth Cazares, Histology Supervisor Department of Pathology Swedish Covenant Hospital Chicago, IL 60625 *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. From JWEEMS <@t> sjha.org Wed Jul 25 09:57:00 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Jul 25 09:57:27 2007 Subject: [Histonet] Use of molecular sieve beads In-Reply-To: <913FAC2B773C19488E26AE6572180FA509BF0241@exch01.schosp.org> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FF9F52@sjhaexc02.sjha.org> Yes, used it for years. It works in xylene, absolute alcohol and xylene substitutes. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Cazares, Ruth Sent: Wednesday, July 25, 2007 10:49 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Use of molecular sieve beads Hello histonetters, I have just read an article about molecular sieve beads being used in xylene to extract the water, and therefore extending the life of the xylene indefinitely. This article was given to me by a cytologist. She came across it on the cytology equivalent to the Histonet. So my question is, has anybody in histology used this method to keep xylene moisture free? And if so, do it really extend the life of the xylene indefinitely? Any and all information is greatly appreciated! Ruth Cazares, Histology Supervisor Department of Pathology Swedish Covenant Hospital Chicago, IL 60625 *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From Terry.Marshall <@t> rothgen.nhs.uk Wed Jul 25 10:01:13 2007 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Wed Jul 25 10:02:08 2007 Subject: [Histonet] Use of molecular sieve beads Message-ID: <5C0BED61F529364E86309CADEA63FEF25E6CA4@TRFT-EX01.xRothGen.nhs.uk> That's two questions:-) Which one did Joyce answer? :-) Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cazares, Ruth Sent: 25 July 2007 15:49 To: histonet@pathology.swmed.edu Subject: [Histonet] Use of molecular sieve beads Hello histonetters, I have just read an article about molecular sieve beads being used in xylene to extract the water, and therefore extending the life of the xylene indefinitely. This article was given to me by a cytologist. She came across it on the cytology equivalent to the Histonet. So my question is, has anybody in histology used this method to keep xylene moisture free? And if so, do it really extend the life of the xylene indefinitely? Any and all information is greatly appreciated! Ruth Cazares, Histology Supervisor Department of Pathology Swedish Covenant Hospital Chicago, IL 60625 *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Jul 25 10:03:56 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jul 25 10:04:03 2007 Subject: [Histonet] Use of molecular sieve beads In-Reply-To: <913FAC2B773C19488E26AE6572180FA509BF0241@exch01.schosp.org> Message-ID: <658251.76237.qm@web61212.mail.yahoo.com> Ruth: Xylene is usually mixed with ethanol and some amount of water during staining, and with ethanol, water and tissue components (specialy fat) during processing, which means that it is not only water what the xylene mixes with, UNLESS you are referring to xylene just in storage before being used for any purpose. USED xylene (i.e. the one used in tissue processing or staining) cannot be "purified" with molecular sieve beads, and the one in storage will only requiere tighten caps and short storage times. Used xylene can be "purified" only by distillation, that will eliminate both water and ethanol, and will produce a "waste" containing oils (from animal tissues and paraffin waxes). I would not attemp using molecular sieve beads as a routine procedure. Ren? J. "Cazares, Ruth" wrote: Hello histonetters, I have just read an article about molecular sieve beads being used in xylene to extract the water, and therefore extending the life of the xylene indefinitely. This article was given to me by a cytologist. She came across it on the cytology equivalent to the Histonet. So my question is, has anybody in histology used this method to keep xylene moisture free? And if so, do it really extend the life of the xylene indefinitely? Any and all information is greatly appreciated! Ruth Cazares, Histology Supervisor Department of Pathology Swedish Covenant Hospital Chicago, IL 60625 *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Shape Yahoo! in your own image. Join our Network Research Panel today! From JWEEMS <@t> sjha.org Wed Jul 25 10:06:02 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Jul 25 10:06:30 2007 Subject: [Histonet] Use of molecular sieve beads In-Reply-To: <5C0BED61F529364E86309CADEA63FEF25E6CA4@TRFT-EX01.xRothGen.nhs.uk> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FF9F55@sjhaexc02.sjha.org> Picky, picky... used it - it works!!! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Wednesday, July 25, 2007 11:01 AM To: Cazares, Ruth; histonet@pathology.swmed.edu Subject: RE: [Histonet] Use of molecular sieve beads That's two questions:-) Which one did Joyce answer? :-) Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cazares, Ruth Sent: 25 July 2007 15:49 To: histonet@pathology.swmed.edu Subject: [Histonet] Use of molecular sieve beads Hello histonetters, I have just read an article about molecular sieve beads being used in xylene to extract the water, and therefore extending the life of the xylene indefinitely. This article was given to me by a cytologist. She came across it on the cytology equivalent to the Histonet. So my question is, has anybody in histology used this method to keep xylene moisture free? And if so, do it really extend the life of the xylene indefinitely? Any and all information is greatly appreciated! Ruth Cazares, Histology Supervisor Department of Pathology Swedish Covenant Hospital Chicago, IL 60625 *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From derek.papalegis <@t> tufts.edu Wed Jul 25 10:21:09 2007 From: derek.papalegis <@t> tufts.edu (Derek Papalegis) Date: Wed Jul 25 10:21:18 2007 Subject: [Histonet] hemoglobin Message-ID: <46A76A65.6010908@tufts.edu> Hi All, An investigator has asked me to stain some sections for hemoglobin. I have already provided him with an iron stain and he wants to go further with it. Can anyone recommend a stain specifically for hemoglobin? I have found some for hemosiderin but I am unsure as to if they will be sufficient. If someone could let me know what stain they use and what the procedure is I would greatly appreciate it. Thanks! -Derek -- Derek Papalegis HT (ASCP) Histotechnician Division of Laboratory Animal Medicine Tufts University 136 Harrison Avenue Boston, MA 02111 phone: 617 636-2971 fax: 617 636-8354 From mcauliff <@t> umdnj.edu Wed Jul 25 10:45:48 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Jul 25 10:46:27 2007 Subject: [Histonet] hemoglobin In-Reply-To: <46A76A65.6010908@tufts.edu> References: <46A76A65.6010908@tufts.edu> Message-ID: <46A7702C.9000002@umdnj.edu> There are several stains for hemoglobin. I recall using benzidine in the past but the details escape me at the moment. You can stain hemoglobin red and hemosiderin blue with a method developed by Puchtler and Sweat (Archiv. Pathol. 75:588, 1963). A simpler stain is the Dunn-Thompson method (Archiv. Pathol. 39:49, 1945). Geoff Geoff Derek Papalegis wrote: > Hi All, > An investigator has asked me to stain some sections for hemoglobin. I > have already provided him with an iron stain and he wants to go > further with it. Can anyone recommend a stain specifically for > hemoglobin? I have found some for hemosiderin but I am unsure as to if > they will be sufficient. If someone could let me know what stain they > use and what the procedure is I would greatly appreciate it. > > Thanks! > -Derek > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From Rcartun <@t> harthosp.org Wed Jul 25 10:49:49 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Jul 25 10:50:18 2007 Subject: [Histonet] hemoglobin In-Reply-To: <46A76A65.6010908@tufts.edu> References: <46A76A65.6010908@tufts.edu> Message-ID: <46A738DE020000770000717C@gwmail.harthosp.org> You can do IHC for hemoglobin. We use a polyclonal antibody from Dako (A0118). Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> Derek Papalegis 07/25/07 11:21 AM >>> Hi All, An investigator has asked me to stain some sections for hemoglobin. I have already provided him with an iron stain and he wants to go further with it. Can anyone recommend a stain specifically for hemoglobin? I have found some for hemosiderin but I am unsure as to if they will be sufficient. If someone could let me know what stain they use and what the procedure is I would greatly appreciate it. Thanks! -Derek -- Derek Papalegis HT (ASCP) Histotechnician Division of Laboratory Animal Medicine Tufts University 136 Harrison Avenue Boston, MA 02111 phone: 617 636-2971 fax: 617 636-8354 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Jul 25 10:50:08 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jul 25 10:50:21 2007 Subject: [Histonet] hemoglobin In-Reply-To: <46A76A65.6010908@tufts.edu> Message-ID: <835835.36155.qm@web61220.mail.yahoo.com> Derek: There is a method (Ralph, 1941) that uses 1% benzidine. I would NOT use since benzidine is highly toxic. Goulliart (1941) uses acetic acid with KI to later examine under polarized light (looking for the so called Teichmann crystals). Dunn (1946) stains frozen sections with a rippen solution of cyanol in acidified water, counterstained with an acid safranin solution. O'Brien (1961) describes a method using the hemoglobin catalase reaction with peroxide and o-dianisidine. All these methods could make "havoc" into your well structured and "safe" routine. Try to convince your investigator that Prussian blue is enough (that is what I would do). Ren? J. Derek Papalegis wrote: Hi All, An investigator has asked me to stain some sections for hemoglobin. I have already provided him with an iron stain and he wants to go further with it. Can anyone recommend a stain specifically for hemoglobin? I have found some for hemosiderin but I am unsure as to if they will be sufficient. If someone could let me know what stain they use and what the procedure is I would greatly appreciate it. Thanks! -Derek -- Derek Papalegis HT (ASCP) Histotechnician Division of Laboratory Animal Medicine Tufts University 136 Harrison Avenue Boston, MA 02111 phone: 617 636-2971 fax: 617 636-8354 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get the free Yahoo! toolbar and rest assured with the added security of spyware protection. From PMonfils <@t> Lifespan.org Wed Jul 25 11:23:07 2007 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Jul 25 11:23:54 2007 Subject: [Histonet] hemoglobin In-Reply-To: <46A76A65.6010908@tufts.edu> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273CB6@LSRIEXCH1.lsmaster.lifespan.org> I have used the Okajima Technique successfully, to demonstrate that large round inclusions in the cytoplasm of large mysterious cells in a skin lesion were in fact phagocytized red blood cells, based on hemoglobin staining, thereby identifying the mystery cells as parasitic amebae. It's a pretty simple technique, and while I have not used it much, it worked well on the couple of occasions when I did use it. I can send it if you don't have it. From thoward <@t> unm.edu Wed Jul 25 11:34:45 2007 From: thoward <@t> unm.edu (Tamara A Howard) Date: Wed Jul 25 11:35:01 2007 Subject: [Histonet] RE: microtomy instruction resources Message-ID: Does NMSH have any kind of instructional videos? I know the education outreach "arm" of MSA has a microtomy video, but I've never actually watched the thing & don't know if it covers paraffin/cryostat sectioning or just ultramicrotomy. Check under education; there is a video list. http://www.microscopy.org/ Good luck! Tamara *************************** Tamara Howard Cell Biology & Physiology UNM-HSC Albuquerque, NM *************************** From oshel1pe <@t> cmich.edu Wed Jul 25 11:47:07 2007 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Wed Jul 25 11:47:31 2007 Subject: [Histonet] RE: microtomy instruction resources In-Reply-To: References: Message-ID: The MSA video is on ultramicrotomy. Useful, but not a waxy show. Phil >Does NMSH have any kind of instructional videos? I know the >education outreach "arm" of MSA has a microtomy video, but I've >never actually watched the thing & don't know if it covers >paraffin/cryostat sectioning or just ultramicrotomy. Check under >education; there is a video list. > >http://www.microscopy.org/ > >Good luck! > >Tamara > >*************************** >Tamara Howard >Cell Biology & Physiology >UNM-HSC >Albuquerque, NM >*************************** -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 From PIXLEYSK <@t> UCMAIL.UC.EDU Wed Jul 25 12:26:26 2007 From: PIXLEYSK <@t> UCMAIL.UC.EDU (Pixley, Sarah (pixleysk)) Date: Wed Jul 25 12:26:33 2007 Subject: [Histonet] RE: OCT tissuetek In-Reply-To: <200707251602.BEV97668@mprelay3.uc.edu> Message-ID: Dear Sonny Duong: It appears that your antigen does not withstand the freezing. That may be unusual, but I am not surprised - have seen odder things. I can think of a couple of things to try; 1) Do you save your samples in air-tight containers? i.e. a Zip-loc type bag? This, plus putting a few pieces of wet ice into the bag can prevent freeze-drying from occurring, which could be part of the problem. 2) For long term storage, we now keep our tissues in a cryoprotectant solution in the refrigerator (then soak them in sucrose and freeze them one day before cutting). This preserves all of the antigens we have worked with to date. The person that I got this procedure from has had preservation of antigenicity for at least a year in some cases. But this may not work for you because of the lag between taking out of cryoprotectant and freezing. See procedure below. Another hint: After sectioning a frozen block, we thaw the tissue and reimmerse in sucrose and then if we need the tissue again, we refreeze and cut. This actually works better than putting the frozen block back in the freezer and cutting later (it gets too hard and brittle). Antigenicity of BrdU has not been affected, but we haven't tested anything else this way. 3) If you are just waiting for a few days only, then just keep your tissue in sucrose and freeze just before cutting! Our general procedure is: Fix: Perfuse animal (or fix tissues by immersion), post fix for 2-24 hrs, 4% paraformaldehyde. Rinse with PBS or PB Immerse in cryoprotectant and store in fridge until needed. Immerse in sucrose solution (10-30% sucrose in 0.1M phosphate buffer) for 1-3 days. Immerse in OCT and freeze on dry ice. Cut the next day (if we cut the same day, the samples are brittle from the low temp of the dry ice-but we haven't tried freezing in the cryostat, which should allow same day cutting). Cryoprotectant: (30% ethylene glycol v/v; 30 % sucrose, 0.02% Na Azide, 0.04 M PB) For Total volume of 1 liter, add, in order: 1. 200 ml dd water 2. 200 ml 0.2 M phosphate buffer (final conc. 0.04M PB) 3. 300 ml Ethylene Glycol 4. 10 ml of 2% Na Azide 5. 300 g sucrose: add slowly (~100 g at a time) with stirring until dissolved. 6. Stir until dissolved 7. Check volume, but should be 1 liter. 8. Refrigerate Sarah Pixley From erweber <@t> maxhealth.com Wed Jul 25 12:27:44 2007 From: erweber <@t> maxhealth.com (Eric Weber) Date: Wed Jul 25 12:27:05 2007 Subject: [Histonet] Histo Tech Needed In Texas Message-ID: <9D12D4EF30176D4F839EB47F3D0E8436015A53B1@exbk2.maxhealth.com> Histoneters, One of my facilities in Texas is looking for a histology supervisor. 5 years experience (preferable in immunochemistry) is required as well as ASCP certification. Shift is 6:30 am-3:30pm. Please call me for more details. Thank you in advance. Eric Weber Recruitment Specialist Maxim Staffing Solutions Toll Free (866) 466-2974 Toll Free Fax (866) 466-2981 erweber@maxhealth.com From Mary.Vaughan <@t> RoswellPark.org Wed Jul 25 13:05:23 2007 From: Mary.Vaughan <@t> RoswellPark.org (Vaughan, Mary) Date: Wed Jul 25 13:05:29 2007 Subject: [Histonet] RE: DAKO labels In-Reply-To: Message-ID: <6FF91AE4F1DC7743A6466E334EB865AE1A6E91B1@VERITY.roswellpark.org> Hi Trey - It's funny you should post a question about these labels as we here in our lab were just discussing them this morning. We have 3 older and 1 new DAKO autostainers. I had worked with a rep from Shamrock labels and she custom made some labels for our older autostainer [Seymour] use. They were around $11/roll [Shamrock's price] instead of $90.00/roll [DAKO's price]. They're not solvent resistant and they don't have the protective sheet of plastic on them, but I put them on after I do the staining so that's OK with me[hence the lower price]. As you can imagine, we do quite a volume of slides with 4 machines, AND we're in research so our dollars are quite tight [everybody's dollars are tight these days]. Well, when we rec'd our new DAKO machine I was surprised to see a change in the Seymour labeling system.... It seems that it's been re-designed with a sensor so you have to use their labels. Now you're telling me that they're not available.... What's up with this? I'm still working with the Shamrock rep to try and figure out a new label for use with this sensor containing Seymour labeler. If you come up with an alternative please let me know. For those of you who want the Shamrock info please e-mail me off the list, thanks. Best Regards, Mary Vaughan HT (ASCP) Research Specialist Roswell Park Cancer Institute Sci-601 Elm & Carlton Streets Buffalo, N Y 14263 phone: 716.845.3006 FAX: 716.845.4076 ........................................................................ ............. Message: 4 Date: Tue, 24 Jul 2007 13:58:57 -0400 From: "Richard Bruggeman" Subject: [Histonet] Dako's Seymour Slide Labels To: Message-ID: <46A605A1.48B8.00C6.0@psu.edu> Content-Type: text/plain; charset=US-ASCII I tried to place an order with Dako for Seymour slide labels and printer supplies and Dako claims to be out of this product and can not provide me with an expected ship date or alternate vendor. Has anyone found an alternate source for the printer supplies and slide labels to use with the Dako Seymour slide labeler. Thanks. Trey Bruggeman Milton S. Hershey Medical Center Penn State College of Medicine Department of Pathology 500 University Drive Hershey, PA 17033 (717) 531-1044 This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. From mpence <@t> grhs.net Wed Jul 25 13:31:46 2007 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Jul 25 13:31:54 2007 Subject: [Histonet] RE: DAKO labels In-Reply-To: <6FF91AE4F1DC7743A6466E334EB865AE1A6E91B1@VERITY.roswellpark.org> Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C682@IS-E2K3.grhs.net> Go Ventana! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vaughan, Mary Sent: Wednesday, July 25, 2007 1:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: DAKO labels Hi Trey - It's funny you should post a question about these labels as we here in our lab were just discussing them this morning. We have 3 older and 1 new DAKO autostainers. I had worked with a rep from Shamrock labels and she custom made some labels for our older autostainer [Seymour] use. They were around $11/roll [Shamrock's price] instead of $90.00/roll [DAKO's price]. They're not solvent resistant and they don't have the protective sheet of plastic on them, but I put them on after I do the staining so that's OK with me[hence the lower price]. As you can imagine, we do quite a volume of slides with 4 machines, AND we're in research so our dollars are quite tight [everybody's dollars are tight these days]. Well, when we rec'd our new DAKO machine I was surprised to see a change in the Seymour labeling system.... It seems that it's been re-designed with a sensor so you have to use their labels. Now you're telling me that they're not available.... What's up with this? I'm still working with the Shamrock rep to try and figure out a new label for use with this sensor containing Seymour labeler. If you come up with an alternative please let me know. For those of you who want the Shamrock info please e-mail me off the list, thanks. Best Regards, Mary Vaughan HT (ASCP) Research Specialist Roswell Park Cancer Institute Sci-601 Elm & Carlton Streets Buffalo, N Y 14263 phone: 716.845.3006 FAX: 716.845.4076 ........................................................................ ............. Message: 4 Date: Tue, 24 Jul 2007 13:58:57 -0400 From: "Richard Bruggeman" Subject: [Histonet] Dako's Seymour Slide Labels To: Message-ID: <46A605A1.48B8.00C6.0@psu.edu> Content-Type: text/plain; charset=US-ASCII I tried to place an order with Dako for Seymour slide labels and printer supplies and Dako claims to be out of this product and can not provide me with an expected ship date or alternate vendor. Has anyone found an alternate source for the printer supplies and slide labels to use with the Dako Seymour slide labeler. Thanks. Trey Bruggeman Milton S. Hershey Medical Center Penn State College of Medicine Department of Pathology 500 University Drive Hershey, PA 17033 (717) 531-1044 This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leba <@t> hawaii.edu Wed Jul 25 14:59:42 2007 From: leba <@t> hawaii.edu (Heather A Leba) Date: Wed Jul 25 14:59:52 2007 Subject: [Histonet] histology samples In-Reply-To: <835835.36155.qm@web61220.mail.yahoo.com> References: <46A76A65.6010908@tufts.edu> <835835.36155.qm@web61220.mail.yahoo.com> Message-ID: Hello all, I've been doing histology for only about a year and am in the middle of a study on reproductive biology of goatfish, Mullidae, and most of my stuff has turned out well, with only minor problems. But, my question is this...does anyone know a reliable source to which I can send preserved ovaries to be embedded, sectioned, made into slides and stained with H & E?? I've been doing this myself but it would cut a lot of time out of my project if I could send them out, and unfortunately our university's histological prowess is lacking. A fellow grad student of mine said she'd sent them to Louisiana State I believe, but I cannot get a hold of her. Any suggestions would be welcomed! Thanks! Heather Leba Graduate Student University of Hawaii, Manoa 2538 The Mall, 152 Edmondson Hall Honolulu, HI 96822 ----- Original Message ----- From: Rene J Buesa Date: Wednesday, July 25, 2007 6:28 am Subject: Re: [Histonet] hemoglobin To: Derek Papalegis , histonet@lists.utsouthwestern.edu > Derek: > There is a method (Ralph, 1941) that uses 1% benzidine. I would > NOT use since benzidine is highly toxic. > Goulliart (1941) uses acetic acid with KI to later examine under > polarized light (looking for the so called Teichmann crystals). > Dunn (1946) stains frozen sections with a rippen solution of > cyanol in acidified water, counterstained with an acid safranin > solution. O'Brien (1961) describes a method using the hemoglobin > catalase reaction with peroxide and o-dianisidine. > All these methods could make "havoc" into your well structured > and "safe" routine. Try to convince your investigator that Prussian > blue is enough (that is what I would do). > Ren? J. > > Derek Papalegis wrote: > Hi All, > An investigator has asked me to stain some sections for hemoglobin. > I > have already provided him with an iron stain and he wants to go > further > with it. Can anyone recommend a stain specifically for hemoglobin? > I > have found some for hemosiderin but I am unsure as to if they will > be > sufficient. If someone could let me know what stain they use and > what > the procedure is I would greatly appreciate it. > > Thanks! > -Derek > > -- > Derek Papalegis HT (ASCP) > Histotechnician > Division of Laboratory Animal Medicine > Tufts University > 136 Harrison Avenue > Boston, MA 02111 > phone: 617 636-2971 > fax: 617 636-8354 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Get the free Yahoo! toolbar and rest assured with the added > security of spyware protection. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jflinn <@t> gmu.edu Wed Jul 25 15:22:45 2007 From: jflinn <@t> gmu.edu (Jane M Flinn) Date: Wed Jul 25 15:22:53 2007 Subject: [Histonet] histology samples In-Reply-To: References: <46A76A65.6010908@tufts.edu> <835835.36155.qm@web61220.mail.yahoo.com> Message-ID: Please unsubscibe me to histonet. Thaks jane Flinn "Life is short - make haste to be kind" Dr. Jane Flinn Director, Biopsychology Program George Mason University, 3F5 4400 University Dr. Fairfax, VA 22030 Phone: 703-993-4107 Fax: 703-993-1359 ----- Original Message ----- From: Heather A Leba Date: Wednesday, July 25, 2007 9:59 pm Subject: [Histonet] histology samples > Hello all, > > I've been doing histology for only about a year and am in the > middle of a study on reproductive biology of goatfish, Mullidae, > and most of my stuff has turned out well, with only minor > problems. But, my question is this...does anyone know a reliable > source to which I can send preserved ovaries to be embedded, > sectioned, made into slides and stained with H & E?? I've been > doing this myself but it would cut a lot of time out of my project > if I could send them out, and unfortunately our university's > histological prowess is lacking. A fellow grad student of mine > said she'd sent them to Louisiana State I believe, but I cannot > get a hold of her. Any suggestions would be welcomed! > > Thanks! > > Heather Leba > > Graduate Student > University of Hawaii, Manoa > 2538 The Mall, 152 Edmondson Hall > Honolulu, HI 96822 > > > ----- Original Message ----- > From: Rene J Buesa > Date: Wednesday, July 25, 2007 6:28 am > Subject: Re: [Histonet] hemoglobin > To: Derek Papalegis , > histonet@lists.utsouthwestern.edu > > Derek: > > There is a method (Ralph, 1941) that uses 1% benzidine. I would > > NOT use since benzidine is highly toxic. > > Goulliart (1941) uses acetic acid with KI to later examine > under > > polarized light (looking for the so called Teichmann crystals). > > Dunn (1946) stains frozen sections with a rippen solution of > > cyanol in acidified water, counterstained with an acid safranin > > solution. O'Brien (1961) describes a method using the > hemoglobin > > catalase reaction with peroxide and o-dianisidine. > > All these methods could make "havoc" into your well structured > > and "safe" routine. Try to convince your investigator that > Prussian > > blue is enough (that is what I would do). > > Ren? J. > > > > Derek Papalegis wrote: > > Hi All, > > An investigator has asked me to stain some sections for > hemoglobin. > > I > > have already provided him with an iron stain and he wants to go > > further > > with it. Can anyone recommend a stain specifically for > hemoglobin? > > I > > have found some for hemosiderin but I am unsure as to if they > will > > be > > sufficient. If someone could let me know what stain they use and > > what > > the procedure is I would greatly appreciate it. > > > > Thanks! > > -Derek > > > > -- > > Derek Papalegis HT (ASCP) > > Histotechnician > > Division of Laboratory Animal Medicine > > Tufts University > > 136 Harrison Avenue > > Boston, MA 02111 > > phone: 617 636-2971 > > fax: 617 636-8354 > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > > > --------------------------------- > > Get the free Yahoo! toolbar and rest assured with the added > > security of spyware protection. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From sbruce <@t> vetpathservicesinc.com Wed Jul 25 17:45:24 2007 From: sbruce <@t> vetpathservicesinc.com (Suzanne Bruce) Date: Wed Jul 25 17:45:28 2007 Subject: [Histonet] Technovit 7200 H&E staining Message-ID: <26DB1FDFBF9EE14AB3AACC873519A4A2220D8B@vpss1.VetPathServicesInc.local> I am using Technovit 7200. I have been trying to get a good H&E stain, but have been unsuccessful (the hematoxylin is barely visible). Does anyone have any suggestions or techniques I could try? Thanks in advance Suzanne From Rcartun <@t> harthosp.org Wed Jul 25 19:07:47 2007 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Jul 25 19:08:02 2007 Subject: [Histonet] New Position - Hartford Hospital Message-ID: <46A7AD9302000077000071A6@gwmail.harthosp.org> We are looking for a "Team Leader" for my Immunopathology Laboratory at Hartford Hospital in Hartford, CT. Anyone interested in the position should contact our Anatomic Pathology Manager, Cathy Kosciol, at (860) 545-2466 for additional information and requirements. Or, feel free to contact me as well. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From gervaip <@t> aol.com Wed Jul 25 19:27:53 2007 From: gervaip <@t> aol.com (gervaip@aol.com) Date: Wed Jul 25 19:28:01 2007 Subject: [Histonet] unsubscrbe Message-ID: <8C99D32F0C9F340-C88-2216@WEBMAIL-MC06.sysops.aol.com> ________________________________________________________________________ AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com. From Jason.PALMER <@t> svhm.org.au Wed Jul 25 19:59:50 2007 From: Jason.PALMER <@t> svhm.org.au (PALMER Jason (SVHM)) Date: Wed Jul 25 19:59:58 2007 Subject: [Histonet] Rand D Systems anti rat CD34 on FFPE? Message-ID: Hi all. A look throught the archives gives me the impression that no one has been able to immunohistochemically label CD34 in FFPE rat tissue. We came across recently came across an R and D anti rat CD34 antibody (goat anti rat, AF4117) that they say works for immuno, though no mention of material used. Has anyone perchance tried it on paraffin embedded material? Thanks, Jason Jason Palmer Bernard O'Brien Institute of Microsurgery 42 Fitzroy St, Fitzroy Victoria 3065 Australia tel +61 3 9288 4018 fax +61 3 9416 0926 email: jason.palmer@svhm.org.au Disclaimer : The contents of this e-mail including any attachments are intended only for the person or entity to which this e-mail is addressed and may contain confidential, privileged and/or commercially sensitive material. If you are not, or believe you may not be, the intended recipient, please advise the sender immediately by return e-mail, delete this e-mail and destroy any copies. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From mickie25 <@t> netzero.net Wed Jul 25 21:30:51 2007 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Wed Jul 25 21:31:19 2007 Subject: [Histonet] Use of molecular sieve beads In-Reply-To: <913FAC2B773C19488E26AE6572180FA509BF0241@exch01.schosp.org> References: <913FAC2B773C19488E26AE6572180FA509BF0241@exch01.schosp.org> Message-ID: Hi Ruth, Creative Waste Solutions has a molecular sieve product for extending the life of xylene or xylene substitute on stainers, for the steps at the end, after 100% ethanol. It does work and with two sets, you just rotate dried ones for the wet ones after about 200 to 400 slides. You can call Rex Johnson (no relation to me) 1-503-784-1232. They also have a great product for recycling alcohol and buffered formalin that many labs use and save a ton of money with in reagents they don't have to purchase as often. He can fill you in on the details. Good Luck, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cazares, Ruth Sent: Wednesday, July 25, 2007 7:49 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Use of molecular sieve beads Hello histonetters, I have just read an article about molecular sieve beads being used in xylene to extract the water, and therefore extending the life of the xylene indefinitely. This article was given to me by a cytologist. She came across it on the cytology equivalent to the Histonet. So my question is, has anybody in histology used this method to keep xylene moisture free? And if so, do it really extend the life of the xylene indefinitely? Any and all information is greatly appreciated! Ruth Cazares, Histology Supervisor Department of Pathology Swedish Covenant Hospital Chicago, IL 60625 *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mbmphoto <@t> gmail.com Wed Jul 25 22:58:18 2007 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Wed Jul 25 22:56:45 2007 Subject: [Histonet] need anti-human Neurturin (NTN) protocol for staining primate 40um sections Message-ID: <9A614584-768F-4D71-9B94-D402E4F49BCA@gmail.com> Can someone please send me a protocol for labeling anti-human Neurturin (NTN) on Zamboni fixed cryostat 40um sections (free-floating). We have tried using the NTN antibody from RD Systems (polyclonal anti-goat) & Chemicon's polyclonal without success. Both antibodies work on rat tissue (fixed in 10% NBF), but not the primate sections fixed in Zamboni fixative (can't change the choice of fixative). Any information, suggestions & tips anyone can provide will be greatly appreciated. Maria Bartola Mejia UCSF Department of Neurosurgery SF CA, 94103 From ljwh2 <@t> cam.ac.uk Thu Jul 26 05:24:48 2007 From: ljwh2 <@t> cam.ac.uk (Laura Harris) Date: Thu Jul 26 05:25:03 2007 Subject: [Histonet] help - unsubscribe Message-ID: <46A87670.8030204@cam.ac.uk> please unsubscribe me from the list many thanks From ASelf <@t> gmhsc.com Thu Jul 26 07:06:16 2007 From: ASelf <@t> gmhsc.com (Amy Self) Date: Thu Jul 26 07:06:00 2007 Subject: [Histonet] Decal Message-ID: <39836CD6DB61654E8F95A35898C9218602E4F1A8@exchange.gmhpost.com> Good Morning Histonetters, I was wandering what protocols/procedures where being used for decal of bones? We only decal knee bones and femurs. Here lately we have been having a time.... It seems like nothing we try works. Thanks in advance, Amy Amy Self Georgetown Hospital Systems NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From erweber <@t> maxhealth.com Thu Jul 26 07:29:40 2007 From: erweber <@t> maxhealth.com (Eric Weber) Date: Thu Jul 26 07:28:08 2007 Subject: [Histonet] (no subject) Message-ID: <9D12D4EF30176D4F839EB47F3D0E8436015A53CF@exbk2.maxhealth.com> Hello Histoneters, We are looking for a histology manager/supervisor for a hospital lab in western North Carolina. Recent supervisor or management experience is required. They are willing to take a temp or temp to perm. Great area of NC, please call me for more details. Thank you in advance. Eric Weber Recruitment Specialist Maxim Staffing Solutions Toll Free (866) 466-2974 Toll Free Fax (866) 466-2981 erweber@maxhealth.com From sheila_adey <@t> hotmail.com Thu Jul 26 08:34:37 2007 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Thu Jul 26 08:34:54 2007 Subject: [Histonet] Decal In-Reply-To: <39836CD6DB61654E8F95A35898C9218602E4F1A8@exchange.gmhpost.com> Message-ID: The Dr.s cut a slice of the femur and it is fixed overnight in 10% NBF. The next day we place the cassette in STAT Decal for the 8hr shift. If it is cut to the nice 4mm, this should decal nicely. Sheila Adey HT MLT Port Huron Hospital Michigan >From: "Amy Self" >To: >Subject: [Histonet] Decal >Date: Thu, 26 Jul 2007 08:06:16 -0400 > > > > Good Morning Histonetters, > > I was wandering what protocols/procedures where being used for decal of >bones? We only decal knee bones and femurs. Here lately we have been >having a time.... It seems like nothing we try works. > > Thanks in advance, Amy > > > Amy Self > Georgetown Hospital Systems > > >NOTE: >The information contained in this message may be privileged, >confidential and protected from disclosure. >If the reader of this message is not the intended recipient, >or an employee or agent responsible for delivering this message >to the intended recipient, >you are hereby notified that any dissemination, distribution or >copying of this communication is strictly prohibited. >If you have received this communication in error, >please notify us immediately by replying to this message and >deleting it from your computer. >Thank you. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Put Your Face In Your Space with Windows Live Spaces http://spaces.live.com/?mkt=en-ca From mcauliff <@t> umdnj.edu Thu Jul 26 09:21:46 2007 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Jul 26 09:22:37 2007 Subject: [Histonet] Decal In-Reply-To: <39836CD6DB61654E8F95A35898C9218602E4F1A8@exchange.gmhpost.com> References: <39836CD6DB61654E8F95A35898C9218602E4F1A8@exchange.gmhpost.com> Message-ID: <46A8ADFA.9080908@umdnj.edu> Hi Amy: 5% formic acid works well. Geoff Amy Self wrote: > Good Morning Histonetters, > > I was wandering what protocols/procedures where being used for decal of bones? We only decal knee bones and femurs. Here lately we have been having a time.... It seems like nothing we try works. > > Thanks in advance, Amy > > > Amy Self > Georgetown Hospital Systems > > > NOTE: > The information contained in this message may be privileged, > confidential and protected from disclosure. > If the reader of this message is not the intended recipient, > or an employee or agent responsible for delivering this message > to the intended recipient, > you are hereby notified that any dissemination, distribution or > copying of this communication is strictly prohibited. > If you have received this communication in error, > please notify us immediately by replying to this message and > deleting it from your computer. > Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From gcallis <@t> montana.edu Thu Jul 26 09:54:18 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Jul 26 09:53:58 2007 Subject: Block storage Re: [Histonet] OCT tissuetek In-Reply-To: <4D66F259C30FBE3B842431BE@[192.168.1.136]> References: <4D66F259C30FBE3B842431BE@[192.168.1.136]> Message-ID: <6.0.0.22.1.20070726084808.01b5faf0@gemini.msu.montana.edu> We store our tissue samples for years, 7 years or more - with excellent staining for murine CD markers. After snap freezing, the blocks are left inside the Tissue Tek molds, the sides are cut away from mold edges so the embedded blocks can be put into a 50 ml centrifuge tube, tightly capped. You can also wrap a frozen block (make sure no tissue is left uncovered by the OCT) in aluminum foil, and store in a resealable baggie, or the tube. If you cut a block, be sure to RESEAL the surface (block face) with a thin layer of OCT (put a drop on thumb or tongue depressor stick, and rub across the block face, then remove block from metal disk, wrap in foil, put in tube. You can mark on the outside of the foil to identify the block. At 02:44 PM 7/24/2007, you wrote: >Hi all, > >How long can samples be kept in OCT embedding media in a -80 freezer >before they go bad? Forever? A day? I've been trying to stain for >intramuscular triglyceride droplets with Oil Red O and it seems that after >a couple of days the tissues embedded in OCT stain weaker and eventually >don't stain at all. Any help would be appreciated! > >Sonny Duong >Green Lab >University of Virginia > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From boor <@t> email.cz Thu Jul 26 10:38:00 2007 From: boor <@t> email.cz (Boor Peter) Date: Thu Jul 26 10:38:08 2007 Subject: [Histonet] rat-Col.1 hybridoma Message-ID: <006501c7cf9a$f2eed4c0$ae0b8286@ZION> Hi everybody! I'm searching for a hybridoma producing antibody directed or cross-reactive with rat collagen type 1 (event. type 3). Up till now I haven't found any single one on the internet. I'll be very glad for any suggestions! Peter Peter Boor University Clinic of Aachen, Dept. of Nephrology and Immunology, Pauwelsstr. 30; 52074 Aachen; Germany Home: Kullenhofstr. 54A, App. 549; 52074 Aachen; Germany Mob.: 0049 (0) 177 2345 163 Lab: 0049 (0) 241 80 89670 Fax: 0049 (0) 241 80 82446 ICQ Nr.: 259 368 084 From gcallis <@t> montana.edu Thu Jul 26 10:46:55 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Jul 26 10:46:32 2007 Subject: [Histonet] Decal In-Reply-To: <39836CD6DB61654E8F95A35898C9218602E4F1A8@exchange.gmhpost. com> References: <39836CD6DB61654E8F95A35898C9218602E4F1A8@exchange.gmhpost.com> Message-ID: <6.0.0.22.1.20070726094618.01b91528@gemini.msu.montana.edu> From what species? At 06:06 AM 7/26/2007, you wrote: > Good Morning Histonetters, > > I was wandering what protocols/procedures where being used for > decal of bones? We only decal knee bones and femurs. Here lately we > have been having a time.... It seems like nothing we try works. > > Thanks in advance, Amy > > > Amy Self > Georgetown Hospital Systems Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From gu.lang <@t> gmx.at Thu Jul 26 11:19:12 2007 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Jul 26 11:19:25 2007 Subject: [Histonet] interesting pictures Message-ID: <000001c7cfa0$b4a86400$6412a8c0@dielangs.at> Hi all, I found these interesting pictures of a Russian pathology in the web. http://www.freewebs.com/ruspat1/biospylaboratory.htm Gudrun From nsnwl <@t> neuro.hfh.edu Thu Jul 26 11:28:44 2007 From: nsnwl <@t> neuro.hfh.edu (Nancy Lemke) Date: Thu Jul 26 11:42:54 2007 Subject: [Histonet] interesting pictures Message-ID: I think that everyone who feels underequipped should look at these fascinating photographs. It is like turning the clock back 60+ years. Thank you so much for putting them on Histonet. Nancy Lemke Research Coordinator Hermelin Brain Tumor Center Henry Ford Hospital Detroit -----Original message----- From: "Gudrun Lang" gu.lang@gmx.at Date: Thu, 26 Jul 2007 12:20:11 -0400 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] interesting pictures > Hi all, > > I found these interesting pictures of a Russian pathology in the web. > > http://www.freewebs.com/ruspat1/biospylaboratory.htm > > > > > > Gudrun > > ============================================================================== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================== From lesley.bechtold <@t> jax.org Thu Jul 26 12:12:56 2007 From: lesley.bechtold <@t> jax.org (Lesley Bechtold) Date: Thu Jul 26 12:13:56 2007 Subject: [Histonet] interesting pictures In-Reply-To: Message-ID: <20070726131256398.00000004584@spikey> Could these be real and current????? If so, it's scarey! Lesley S. Bechtold Senior Manager, Histopathology & Microscopy Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Lemke Sent: Thursday, July 26, 2007 12:29 PM To: gu.lang@gmx.at; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] interesting pictures I think that everyone who feels underequipped should look at these fascinating photographs. It is like turning the clock back 60+ years. Thank you so much for putting them on Histonet. Nancy Lemke Research Coordinator Hermelin Brain Tumor Center Henry Ford Hospital Detroit -----Original message----- From: "Gudrun Lang" gu.lang@gmx.at Date: Thu, 26 Jul 2007 12:20:11 -0400 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] interesting pictures > Hi all, > > I found these interesting pictures of a Russian pathology in the web. > > http://www.freewebs.com/ruspat1/biospylaboratory.htm > > > > > > Gudrun > > ============================================================================== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From judi.ford <@t> roche.com Thu Jul 26 12:31:00 2007 From: judi.ford <@t> roche.com (Ford, Judi) Date: Thu Jul 26 12:31:16 2007 Subject: [Histonet] interesting pictures In-Reply-To: <000001c7cfa0$b4a86400$6412a8c0@dielangs.at> References: <000001c7cfa0$b4a86400$6412a8c0@dielangs.at> Message-ID: I passed this website around to my coworkers this morning. We're all really grateful for what we have. It's hard to complain now....... Judi Ford Research Associate III Roche Palo Alto, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Thursday, July 26, 2007 9:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] interesting pictures Hi all, I found these interesting pictures of a Russian pathology in the web. http://www.freewebs.com/ruspat1/biospylaboratory.htm Gudrun From rjbuesa <@t> yahoo.com Thu Jul 26 12:31:31 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jul 26 12:31:43 2007 Subject: [Histonet] interesting pictures In-Reply-To: <20070726131256398.00000004584@spikey> Message-ID: <963034.71351.qm@web61221.mail.yahoo.com> Lesley: These photos I am sure are real and current. I have received similar photos from a Russian colleague some time ago and they reflect how histology is practiced today in the majority of histology labs. There are labs in universities and academic institutions somewhat better equiped, but the general practice is as shown in these pictures. To these conditions you have to add that the salary of one of our colleagues ranges between 112 and 145 US$ per month paid for 21 days of work with 6 hours each. This is equivalent to from 0.88 to 1.15 US$ per hour. This salary can cover betwee 0.83 and 1.08 of the cost of the average bread-basket (US$ 134/month). Add shortages of supplies and other "inconveniences" and you would have to agree that in Russia our colleagues have to be really dedicated to their profession to "persevere" in it. Ren? J. Lesley Bechtold wrote: Could these be real and current????? If so, it's scarey! Lesley S. Bechtold Senior Manager, Histopathology & Microscopy Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Lemke Sent: Thursday, July 26, 2007 12:29 PM To: gu.lang@gmx.at; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] interesting pictures I think that everyone who feels underequipped should look at these fascinating photographs. It is like turning the clock back 60+ years. Thank you so much for putting them on Histonet. Nancy Lemke Research Coordinator Hermelin Brain Tumor Center Henry Ford Hospital Detroit -----Original message----- From: "Gudrun Lang" gu.lang@gmx.at Date: Thu, 26 Jul 2007 12:20:11 -0400 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] interesting pictures > Hi all, > > I found these interesting pictures of a Russian pathology in the web. > > http://www.freewebs.com/ruspat1/biospylaboratory.htm > > > > > > Gudrun > > ============================================================================== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get the free Yahoo! toolbar and rest assured with the added security of spyware protection. From burch007 <@t> mc.duke.edu Thu Jul 26 12:55:45 2007 From: burch007 <@t> mc.duke.edu (James L Burchette) Date: Thu Jul 26 12:55:02 2007 Subject: [Histonet] interesting pictures In-Reply-To: <000001c7cfa0$b4a86400$6412a8c0@dielangs.at> Message-ID: Gudrun and all, Very interesting photographs. Thank you for sharing. It brings back memories. I visited Murmansk Russia in 1991. Between fly fishing trips, my host arraigned for me to visit the Murmansk Regional Medical Center. I was the first foreigner to visit the pathology Department. Among the gifts I shared with them were a 10 oz. case of glass cover slips. You would have thought they were gold. The pathologist and tech's said they would be saved and used on consult slides to send to St. Petersburg. The pathologists desk was located at a window where they would use the sun light to reflect on their microscope mirror... and I had two 'clunker' microscopes back home. Tissues were processed in a 'Dunka' processor, pan embedded and attached to a wooden block and cut on a Sledge microtome. Their water bath was a bowl of water. The mounted tissues were flame dried. Yes, very crude. In comparison to the photographs that Gudrum shared, the Murmansk MC Path Lab was very clean and organized and was apparent the techs and Dr's took pride in their surroundings, but were limited due to lack of resources. I was invited to the home of the pathologist and histotech wife for dinner. While sitting at their table and watching the evening news (just like here!) was President Bush and Gorbachev meeting in Moscow. I had my own summit in Murmansk. We dined on borsch and chicken from the USA called 'Leg's of Bush' by the Russian citizens. I look back on that trip with fond memories of the people I met, places I visited, to much vodka and the beautiful Atlantic salmon I caught. Jim Burchette, HT(ASCP) QIHC "A simple histotech from a little country hospital in North Carolina" "Gudrun Lang" Sent by: histonet-bounces@lists.utsouthwestern.edu 07/26/2007 12:19 PM Please respond to gu.lang@gmx.at To cc Subject [Histonet] interesting pictures Hi all, I found these interesting pictures of a Russian pathology in the web. http://www.freewebs.com/ruspat1/biospylaboratory.htm Gudrun _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jjohnson <@t> crispregional.org Thu Jul 26 13:12:32 2007 From: jjohnson <@t> crispregional.org (Jennifer Johnson) Date: Thu Jul 26 13:13:49 2007 Subject: [Histonet] Lymph Node Message-ID: <000001c7cfb0$89ba7340$2082010a@main.crispregional.org> I am having problems with lymph nodes. First of all, when cutting frozens, my Pathologist says that they are chattering too much. When I am cutting them, they seem to be cutting smoothly with no problem and look good on the slide when I cover slip. I have increased the micron, decreased the micron, warmed for a few seconds with my thumb before cutting and they still appear to chatter. On the same lymph node, additional sections that were NOT frozen are showing the same chatter as well as poor staining with Hematoxylin. Any help would be appreciated! Thank you, Jennifer Johnson P.S. In that Russian Path lab, I noticed that they had three pieces of equipment that were newer than mine (the microtome, cryostat, and embedding center) we just upgraded from the carousel (dip & dunk) rotary processor to a refurbished VIP unit. Fortunately, our hospital will pay for cassettes and we do not have to mix our reagents in old pickle jars. From chris <@t> biocare.net Thu Jul 26 14:56:55 2007 From: chris <@t> biocare.net (Chris Von Vedder) Date: Thu Jul 26 14:56:25 2007 Subject: [Histonet] unsubscribe Message-ID: <000801c7cfbf$1e9f5490$9400640a@biocaredomain.com> I'll be on vacation. Best regards, Chris Von Vedder Technical Sales Representative Biocare Medical LLC chris@biocare.net (800)799-9499, #8021 From bell0036 <@t> mc.duke.edu Thu Jul 26 15:03:49 2007 From: bell0036 <@t> mc.duke.edu (Cynthia C Bell) Date: Thu Jul 26 15:03:01 2007 Subject: [Histonet] (no subject) Message-ID: I will be out of the office starting 07/25/2007 and will not return until 08/06/2007. From Histologia <@t> aol.com Thu Jul 26 15:54:00 2007 From: Histologia <@t> aol.com (Histologia@aol.com) Date: Thu Jul 26 15:54:25 2007 Subject: [Histonet] interesting pictures Message-ID: Lesley I assure you that these photos are a true representation of what is happening in all of the former Soviet Countries. For more information please go to my website that discusses what my husband and I are doing in Ukraine. _www.aump.org_ (http://www.aump.org) All volunteer and non profit. For many years now the two of us travel to the hospitals there to help in any way we can with the pathology departments. Occasionally we will have the good fortune of another expert in other fields join us and help in their small way. Through these people we have seen that all the departments are similar. So we spend our vacation days and all the money we can save to bring the cast offs from America. To them anything is brand new and much cherished. I might add that there is no money or economy now. So they exist off the charity of others. Sincerely Terry ************************************** Get a sneak peek of the all-new AOL at http://discover.aol.com/memed/aolcom30tour From ladylaynah <@t> yahoo.com Thu Jul 26 16:20:25 2007 From: ladylaynah <@t> yahoo.com (Constance McManus) Date: Thu Jul 26 16:20:33 2007 Subject: [Histonet] Use of molecular sieve beads Message-ID: <926555.94989.qm@web37005.mail.mud.yahoo.com> Ruth, My husband -- an electron Microscopist -- uses them all the time for alcohols and other reagents. I've used them before in histology and they really do a great job, but you need to allow time for them to work. I would add mine on a Friday morning and by Monday or Tues of the next week the reagent (I was dehydrating alcohol, not xylene) was fine. You need to use them in a certain ratio to the volume of reagent to be deydrated. Also, there are different kinds of Molec seives used for different purposes. I got mine from Sigma. call them and they can help you out. Connie McManus, HT University of Utah School of Medicine Dept. of Dermatology ----- Original Message ---- From: "Cazares, Ruth" To: histonet@pathology.swmed.edu Sent: Wednesday, July 25, 2007 8:49:03 AM Subject: [Histonet] Use of molecular sieve beads Hello histonetters, I have just read an article about molecular sieve beads being used in xylene to extract the water, and therefore extending the life of the xylene indefinitely. This article was given to me by a cytologist. She came across it on the cytology equivalent to the Histonet. So my question is, has anybody in histology used this method to keep xylene moisture free? And if so, do it really extend the life of the xylene indefinitely? Any and all information is greatly appreciated! Ruth Cazares, Histology Supervisor Department of Pathology Swedish Covenant Hospital Chicago, IL 60625 *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Need a vacation? Get great deals to amazing places on Yahoo! Travel. http://travel.yahoo.com/ From conniegrubaugh <@t> hotmail.com Thu Jul 26 21:32:32 2007 From: conniegrubaugh <@t> hotmail.com (connie grubaugh) Date: Thu Jul 26 21:32:37 2007 Subject: [Histonet] someone selling a linear stainer Message-ID: A couple of days ago someone posted an email about selling a linear stainer. If you could email me privately I would appreciate it. Connie Grubaugh _________________________________________________________________ PC Magazine?s 2007 editors? choice for best web mail?award-winning Windows Live Hotmail. http://imagine-windowslive.com/hotmail/?locale=en-us&ocid=TXT_TAGHM_migration_HMWL_mini_pcmag_0707 From garth <@t> apollosci.co.za Fri Jul 27 01:01:31 2007 From: garth <@t> apollosci.co.za (Garth Jerome) Date: Fri Jul 27 01:02:15 2007 Subject: [Histonet] Decal In-Reply-To: <6.0.0.22.1.20070726094618.01b91528@gemini.msu.montana.edu> Message-ID: <000c01c7d013$95c5c230$7700a8c0@jhb.apollosci.co.za> Hi Gayle You didn't mention the size or species of bones. We are currently using 5% formic acid for decal of human bone. We are using a formic acid / HCL combo for animal bones. Regards Garth Jerome Apollo Scientific cc Telephone : 27-11-466 7666 Fascimile : 27-11-466 7672 Email : garth@apollosci.co.za -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: 26 July 2007 05:47 PM To: Amy Self; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Decal From what species? At 06:06 AM 7/26/2007, you wrote: > Good Morning Histonetters, > > I was wandering what protocols/procedures where being used for > decal of bones? We only decal knee bones and femurs. Here lately we > have been having a time.... It seems like nothing we try works. > > Thanks in advance, Amy > > > Amy Self > Georgetown Hospital Systems Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From louise.renton <@t> gmail.com Fri Jul 27 04:28:34 2007 From: louise.renton <@t> gmail.com (louise renton) Date: Fri Jul 27 04:28:46 2007 Subject: [Histonet] shark histology Message-ID: Dear all (especially fishy people) We are trying to identify some cellular stuctures in sections of newborn shark head. Is there a good histology atlas (online resource even better) that anyone can recommend? We are way out of our depth on this one (ha ha). Unless there is someone out there who wouln't mind helping us if we provide a micrograph???? best regards & have a great weekend -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From asmith <@t> mail.barry.edu Fri Jul 27 07:38:33 2007 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Jul 27 07:38:40 2007 Subject: [Histonet] shark histology In-Reply-To: Message-ID: <7DBCCC1FBC77C94F99F920D0CA6400B61E40D2@exchsrv01.barrynet.barry.edu> Some years ago I was doing some work on shark anatomy. I found that the only serious book on either the gross anatomy or the histology of sharks was THE ELASMOBRANCH FISHES by J. Frank Daniel (University of California Press, 1934). There have about a dozen papers since then, mostly in obscure journals. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise renton Sent: Friday, July 27, 2007 5:29 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] shark histology Dear all (especially fishy people) We are trying to identify some cellular stuctures in sections of newborn shark head. Is there a good histology atlas (online resource even better) that anyone can recommend? We are way out of our depth on this one (ha ha). Unless there is someone out there who wouln't mind helping us if we provide a micrograph???? best regards & have a great weekend -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Jul 27 07:43:32 2007 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Jul 27 07:43:38 2007 Subject: [Histonet] shark histology Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222EA3F@wahtntex2.waht.swest.nhs.uk> I thought Lawyers had the same anatomy as human beings? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; If the world seems cold to you, kindle fires to warm it. --Lucy Larcom This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From jnocito <@t> satx.rr.com Fri Jul 27 07:47:05 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Jul 27 07:47:16 2007 Subject: [Histonet] shark histology References: Message-ID: <001201c7d04c$3d27d120$d49eae18@yourxhtr8hvc4p> are we talking about lawyers here? Sorry, couldn't resist. Must be Friday. JTT ----- Original Message ----- From: "louise renton" To: Sent: Friday, July 27, 2007 4:28 AM Subject: [Histonet] shark histology > Dear all (especially fishy people) > > > We are trying to identify some cellular stuctures in sections of newborn > shark head. Is there a good histology atlas (online resource even better) > that anyone can recommend? We are way out of our depth on this one (ha > ha). > Unless there is someone out there who wouln't mind helping us if we > provide > a micrograph???? > > > best regards & have a great weekend > > -- > Louise Renton > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > "There are nights when the wolves are silent and only the moon howls". > George Carlin > No trees were killed in the sending of this message. > However, many electrons were terribly inconvenienced. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Robinsoc <@t> mercyhealth.com Fri Jul 27 08:08:59 2007 From: Robinsoc <@t> mercyhealth.com (Cindy Robinson) Date: Fri Jul 27 08:09:35 2007 Subject: [Histonet] shark histology In-Reply-To: <001201c7d04c$3d27d120$d49eae18@yourxhtr8hvc4p> References: <001201c7d04c$3d27d120$d49eae18@yourxhtr8hvc4p> Message-ID: <46A9A81B020000AF00001496@nodcmsngwia1.trinity-health.org> I'm a histotech with a daughter who is a lawyer. She has heard me and my husband (who is an engineer) rant and rave about lawyers and frivolous lawsuits during her formative years. Needless to say, when she gets a client who wants to proceed with legal action for questionable reasons she tries to counsel them more like she is a psychologist rather than an attorney. Lawyers get a bad rep mostly because ethical does not equal moral values. There are a few who try to blend the ethical with the moral. Just my 2 cents. Cindi Robinson HT(ASCP) MMC-Sioux City Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 >>> "Joe Nocito" 7/27/2007 7:47 AM >>> are we talking about lawyers here? Sorry, couldn't resist. Must be Friday. JTT ----- Original Message ----- From: "louise renton" To: Sent: Friday, July 27, 2007 4:28 AM Subject: [Histonet] shark histology > Dear all (especially fishy people) > > > We are trying to identify some cellular stuctures in sections of newborn > shark head. Is there a good histology atlas (online resource even better) > that anyone can recommend? We are way out of our depth on this one (ha > ha). > Unless there is someone out there who wouln't mind helping us if we > provide > a micrograph???? > > > best regards & have a great weekend > > -- > Louise Renton > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > "There are nights when the wolves are silent and only the moon howls". > George Carlin > No trees were killed in the sending of this message. > However, many electrons were terribly inconvenienced. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ryaskovich <@t> dir.nidcr.nih.gov Fri Jul 27 08:21:09 2007 From: ryaskovich <@t> dir.nidcr.nih.gov (Yaskovich, Ruth A (NIH/NIDCR) [E]) Date: Fri Jul 27 08:21:16 2007 Subject: [Histonet] shark histology In-Reply-To: <46A9A81B020000AF00001496@nodcmsngwia1.trinity-health.org> References: <001201c7d04c$3d27d120$d49eae18@yourxhtr8hvc4p> <46A9A81B020000AF00001496@nodcmsngwia1.trinity-health.org> Message-ID: Cindy, I agree and when you need one. They are wonderful. Ruth Yaskovich N.I.H. -----Original Message----- From: Cindy Robinson [mailto:Robinsoc@mercyhealth.com] Sent: Friday, July 27, 2007 9:09 AM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] shark histology I'm a histotech with a daughter who is a lawyer. She has heard me and my husband (who is an engineer) rant and rave about lawyers and frivolous lawsuits during her formative years. Needless to say, when she gets a client who wants to proceed with legal action for questionable reasons she tries to counsel them more like she is a psychologist rather than an attorney. Lawyers get a bad rep mostly because ethical does not equal moral values. There are a few who try to blend the ethical with the moral. Just my 2 cents. Cindi Robinson HT(ASCP) MMC-Sioux City Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 >>> "Joe Nocito" 7/27/2007 7:47 AM >>> are we talking about lawyers here? Sorry, couldn't resist. Must be Friday. JTT ----- Original Message ----- From: "louise renton" To: Sent: Friday, July 27, 2007 4:28 AM Subject: [Histonet] shark histology > Dear all (especially fishy people) > > > We are trying to identify some cellular stuctures in sections of newborn > shark head. Is there a good histology atlas (online resource even better) > that anyone can recommend? We are way out of our depth on this one (ha > ha). > Unless there is someone out there who wouln't mind helping us if we > provide > a micrograph???? > > > best regards & have a great weekend > > -- > Louise Renton > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > "There are nights when the wolves are silent and only the moon howls". > George Carlin > No trees were killed in the sending of this message. > However, many electrons were terribly inconvenienced. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Fri Jul 27 08:56:14 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Fri Jul 27 08:56:20 2007 Subject: [Histonet] shark histology In-Reply-To: References: Message-ID: How about "The Insides of the Great White Shark" by A Jonah?. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise renton Sent: 27 July 2007 10:29 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] shark histology Dear all (especially fishy people) We are trying to identify some cellular stuctures in sections of newborn shark head. Is there a good histology atlas (online resource even better) that anyone can recommend? We are way out of our depth on this one (ha ha). Unless there is someone out there who wouln't mind helping us if we provide a micrograph???? best regards & have a great weekend -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Fri Jul 27 09:19:53 2007 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri Jul 27 09:20:01 2007 Subject: [Histonet] Ribboning problems Message-ID: <4D14F0FC9316DD41972D5F03C070908B8F45AA@nmdamailsvr.nmda.ad.nmsu.edu> I need some help! I have a Leitz 1512 with over 100,000 miles on it (my new microtome's "in the mail"). Suddenly I get a full face followed by a compression on the left side of the ribbon, then another full face, compression, etc., etc. The 'tome is lubed and the track greased; knife holder firmly set; back of knife clean; phase of moon in sync with planetary alignment. I have to cut at 5 microns to begin with because 4 no longer gives anything but an accordion pleat. What am I missing??? Help!! I still have 60 blocks to cut and get out before 9:30! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From AGrobe2555 <@t> aol.com Fri Jul 27 10:31:14 2007 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Fri Jul 27 10:31:35 2007 Subject: [Histonet] Fat issues Message-ID: Hello All, I have some vascular graft tissues that we have paraffin embedded and some of the tissues have extra-vascular fat. The practice sample I sectioned this morning seems to section well (other than the suture issues), however the fat "blows up" as soon as I place the sections into the flotation bath. Is there some way to minimize this? I will try cooling down the water bath temperature, as it may have been a bit warm. Any suggestions are welcome. Thanks, Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital ************************************** Get a sneak peek of the all-new AOL at http://discover.aol.com/memed/aolcom30tour From asachau <@t> titanmed.com Fri Jul 27 10:39:35 2007 From: asachau <@t> titanmed.com (April Sachau) Date: Fri Jul 27 10:41:07 2007 Subject: [Histonet] Histology Positions In-Reply-To: Message-ID: <7E3ACD48BA6E26408F3188FBF08693F7AC920B@titansbs1.corp.titanmed.com> Hello Histonetters! I have three Histology temporary positions available in Minnesota currently. All travel expenses paid (Airfare, food, housing and car) if you have ever thought about traveling, or know someone that might be interested, please let me know! April Sachau Titan Medical Group Staff Supervisor Phone (866) 332-9600 Ext. 1023 Fax (402) 332-5181 asachau@titanmed.com see us on the web at www.titanmed.com From jnocito <@t> satx.rr.com Fri Jul 27 11:15:08 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Jul 27 11:15:31 2007 Subject: [Histonet] shark histology References: Message-ID: <002801c7d069$52475ae0$d49eae18@yourxhtr8hvc4p> that book bites. JTT ----- Original Message ----- From: "Edwards, R.E." To: "louise renton" ; Sent: Friday, July 27, 2007 8:56 AM Subject: RE: [Histonet] shark histology How about "The Insides of the Great White Shark" by A Jonah?. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise renton Sent: 27 July 2007 10:29 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] shark histology Dear all (especially fishy people) We are trying to identify some cellular stuctures in sections of newborn shark head. Is there a good histology atlas (online resource even better) that anyone can recommend? We are way out of our depth on this one (ha ha). Unless there is someone out there who wouln't mind helping us if we provide a micrograph???? best regards & have a great weekend -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Fri Jul 27 11:20:05 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Jul 27 11:20:10 2007 Subject: [Histonet] Fat issues References: Message-ID: <002e01c7d069$fe979940$d49eae18@yourxhtr8hvc4p> Albert, could be the fat tissues didn't completely process. I read about a technique a few years ago (I can't remember the author,sorry) where you melt the block done, put the tissue between several pieces of paper towels and gently press on the tissue to expel any remaining xylene and re-embed. I did this for a couple of colon cases and it worked for me. It beats reprocessing the entire block. JTT ----- Original Message ----- From: To: Sent: Friday, July 27, 2007 10:31 AM Subject: [Histonet] Fat issues > Hello All, > I have some vascular graft tissues that we have paraffin embedded and some > of the tissues have extra-vascular fat. The practice sample I sectioned > this > morning seems to section well (other than the suture issues), however the > fat > "blows up" as soon as I place the sections into the flotation bath. Is > there some way to minimize this? I will try cooling down the water bath > temperature, as it may have been a bit warm. > > Any suggestions are welcome. > Thanks, > Albert > > Albert C. Grobe, PhD > International Heart Institute of Montana Foundation > Tissue Engineering Lab, Saint Patrick Hospital > > > > > > ************************************** Get a sneak peek of the all-new AOL > at > http://discover.aol.com/memed/aolcom30tour > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jmahoney <@t> alegent.org Fri Jul 27 11:30:06 2007 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Fri Jul 27 11:30:28 2007 Subject: [Histonet] shark histology In-Reply-To: <002801c7d069$52475ae0$d49eae18@yourxhtr8hvc4p> References: <002801c7d069$52475ae0$d49eae18@yourxhtr8hvc4p> Message-ID: <46A9D73E0200003C00015019@gwia.alegent.org> It is now officially Friday. Good one Jo. Actually I thought it was a book I could really sink my teeth into. Jan Omaha >>> "Joe Nocito" 07/27/2007 11:15 AM >>> that book bites. JTT ----- Original Message ----- From: "Edwards, R.E." To: "louise renton" ; Sent: Friday, July 27, 2007 8:56 AM Subject: RE: [Histonet] shark histology How about "The Insides of the Great White Shark" by A Jonah?. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise renton Sent: 27 July 2007 10:29 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] shark histology Dear all (especially fishy people) We are trying to identify some cellular stuctures in sections of newborn shark head. Is there a good histology atlas (online resource even better) that anyone can recommend? We are way out of our depth on this one (ha ha). Unless there is someone out there who wouln't mind helping us if we provide a micrograph???? best regards & have a great weekend -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ladylaynah <@t> yahoo.com Fri Jul 27 12:41:04 2007 From: ladylaynah <@t> yahoo.com (Constance McManus) Date: Fri Jul 27 12:41:09 2007 Subject: [Histonet] interesting pictures Message-ID: <766412.31625.qm@web37005.mail.mud.yahoo.com> gudrun thank you for sharing these photos! One of the PAs in our dept lived in Latvia for 2 years and he has interesting stories about the decrepit and poor condition the infrastructural elements are in the former USSR countries. It's not just medicine, it's also construction, manufacturing, agriculture, etc. And, from what I understand, it's not getting better. Sadly, my first impulse was to laugh at these pictures. I say "sadly" because one should not mock another's misfortune. The more I looked at those picutres, then glanced around the lab I work in, I began to feel a sense of gratitude for all that I generally take fore granted. My heart is filled with compassion for those people over there and I salute all of you who are donating your time, talents & expertise, money, your lives to help the people from these countries make a better life. If the day ever comes that I have the means to do something like that, I would hop like a frog on a hot pan to do it. thanks for opening my eyes to what I have!!! Connie McManus, HT University of Utah School of Medicine Dept. of Dermatology ----- Original Message ---- From: Nancy Lemke To: gu.lang@gmx.at; histonet@lists.utsouthwestern.edu Sent: Thursday, July 26, 2007 10:28:44 AM Subject: Re: [Histonet] interesting pictures I think that everyone who feels underequipped should look at these fascinating photographs. It is like turning the clock back 60+ years. Thank you so much for putting them on Histonet. Nancy Lemke Research Coordinator Hermelin Brain Tumor Center Henry Ford Hospital Detroit -----Original message----- From: "Gudrun Lang" gu.lang@gmx.at Date: Thu, 26 Jul 2007 12:20:11 -0400 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] interesting pictures > Hi all, > > I found these interesting pictures of a Russian pathology in the web. > > http://www.freewebs.com/ruspat1/biospylaboratory.htm > > > > > > Gudrun > > ============================================================================== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Building a website is a piece of cake. Yahoo! Small Business gives you all the tools to get online. http://smallbusiness.yahoo.com/webhosting From sbreeden <@t> nmda.nmsu.edu Fri Jul 27 13:17:09 2007 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri Jul 27 13:17:17 2007 Subject: [Histonet] Ribboning Problem Figgered Out! Message-ID: <4D14F0FC9316DD41972D5F03C070908B8F45B9@nmdamailsvr.nmda.ad.nmsu.edu> First of all, thank you to all who offered their input on my ribboning problem. The advice and counsel was very much appreciated and I looked into every solution - so my warm fuzzy thanks! I did finally figure out what was going on. Despite the high mileage vehicle and the moon phases, as I was disassembling it for its usual Friday Cleaning and Putting Away for the Weekend, I discovered that I had somehow earlier this week bumped the KNIFE HOLDER against the chuck, giving me a tiny indention at each side of that part in the center of the knife holder that... well, I'm sure it has a name but I don't know what it is. I could not see these dents until I took off that little unnamed part to clean it and there they were! I always have a new knife holder squirreled away, so I am positive that will solve the problems I was anticipating on Monday! My only unpleasant anticipation for Monday is telling The Boss I need a new knife holder... gulp! Wish I could blame this on another tech, but I am IT! Rats and mice! Too bad there's not some entrepreneur out there that can machine out those little denty things, thereby saving about $650 for a new one! By the way, JTT recommended an alcoholic tincture applied to the technician to solve the problem.... Darn! I'm out of it! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From vazquezr <@t> ohsu.edu Fri Jul 27 14:00:27 2007 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Jul 27 14:00:52 2007 Subject: [Histonet] Ribboning Problem Figgered Out! Message-ID: Sara, If it is on the knife holder, take your dremel and lightly sand or buff the nicks out...hope it works before you have to buy a new one... Robyn OHSU >>> "Breeden, Sara" 7/27/2007 11:17 AM >>> First of all, thank you to all who offered their input on my ribboning problem. The advice and counsel was very much appreciated and I looked into every solution - so my warm fuzzy thanks! I did finally figure out what was going on. Despite the high mileage vehicle and the moon phases, as I was disassembling it for its usual Friday Cleaning and Putting Away for the Weekend, I discovered that I had somehow earlier this week bumped the KNIFE HOLDER against the chuck, giving me a tiny indention at each side of that part in the center of the knife holder that... well, I'm sure it has a name but I don't know what it is. I could not see these dents until I took off that little unnamed part to clean it and there they were! I always have a new knife holder squirreled away, so I am positive that will solve the problems I was anticipating on Monday! My only unpleasant anticipation for Monday is telling The Boss I need a new knife holder... gulp! Wish I could blame this on another tech, but I am IT! Rats and mice! Too bad there's not some entrepreneur out there that can machine out those little denty things, thereby saving about $650 for a new one! By the way, JTT recommended an alcoholic tincture applied to the technician to solve the problem.... Darn! I'm out of it! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jeanne.reis <@t> biogenidec.com Fri Jul 27 17:54:49 2007 From: jeanne.reis <@t> biogenidec.com (Jeanne Reis) Date: Fri Jul 27 17:55:00 2007 Subject: [Histonet] Jeanne Reis is out of the office. Message-ID: I will be out of the office starting 07/27/2007 and will not return until 07/31/2007. I will respond to your message when I return. From ralphpu <@t> alleninstitute.org Fri Jul 27 18:36:58 2007 From: ralphpu <@t> alleninstitute.org (Ralph Puchalski) Date: Fri Jul 27 18:37:02 2007 Subject: [Histonet] mouse spinal cord atlas Message-ID: I am looking for an image or sketch of the mouse cord and column showing the relationship of the cord to the column, and possibly to the spinal nerves. I have a textbook sketch for human, which shows for example that L1 (the first of the lumbar segment) of the cord corresponds in space (is adjacent) to T11 (the eleventh of the thoracic segment) of the ventral column. Does anyone have or know where I could find such a picture? The atlases I have seen don't have the entire cord or column, just tissue sections cut periodically throughout the cord. Ralph Ralph B. Puchalski, Ph.D. Research Alliance Manager Allen Institute for Brain Science 551 N. 34th Street, Suite 200 Seattle, WA 98103 ralphpu@alleninstitute.org Tel: 206-548-7041 Fax: 206-548-7083 www.brain-map.org , www.alleninstitute.org From mickie25 <@t> netzero.net Fri Jul 27 20:11:59 2007 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Fri Jul 27 20:12:17 2007 Subject: [Histonet] Fat issues In-Reply-To: <002e01c7d069$fe979940$d49eae18@yourxhtr8hvc4p> References: <002e01c7d069$fe979940$d49eae18@yourxhtr8hvc4p> Message-ID: Hello Albert, Joe and all you dedicated Histonetters! Happy Friday! I wrote an article in the Histo-Logic about 4 years ago, (It is in the archives) about a very useful technique for coping with this problem. Here it is in a nut shell. 1. Melt the block down. Save the cassette 2. Blot off all wax. Don't press as this distorts the tissue 3. Put the specimen back in its original cassette and put a lid on. 4. Put it in the with the current days run starting in formalin. 5. Process as usual. 6. embed as usual. You will find that the under processed part is now processed and the part that was ok to begin with will not be harmed by the reprocessing. That part was protected by the paraffin that had infiltrated it originally. Good Luck. Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Friday, July 27, 2007 9:20 AM To: AGrobe2555@aol.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Fat issues Albert, could be the fat tissues didn't completely process. I read about a technique a few years ago (I can't remember the author,sorry) where you melt the block done, put the tissue between several pieces of paper towels and gently press on the tissue to expel any remaining xylene and re-embed. I did this for a couple of colon cases and it worked for me. It beats reprocessing the entire block. JTT ----- Original Message ----- From: To: Sent: Friday, July 27, 2007 10:31 AM Subject: [Histonet] Fat issues > Hello All, > I have some vascular graft tissues that we have paraffin embedded and some > of the tissues have extra-vascular fat. The practice sample I sectioned > this > morning seems to section well (other than the suture issues), however the > fat > "blows up" as soon as I place the sections into the flotation bath. Is > there some way to minimize this? I will try cooling down the water bath > temperature, as it may have been a bit warm. > > Any suggestions are welcome. > Thanks, > Albert > > Albert C. Grobe, PhD > International Heart Institute of Montana Foundation > Tissue Engineering Lab, Saint Patrick Hospital > > > > > > ************************************** Get a sneak peek of the all-new AOL > at > http://discover.aol.com/memed/aolcom30tour > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sjchtascp <@t> yahoo.com Fri Jul 27 20:31:08 2007 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Fri Jul 27 20:31:12 2007 Subject: [Histonet] mayers bluing Message-ID: <464443.40361.qm@web38210.mail.mud.yahoo.com> I just started working in a Derm. lab that uses Mayers, 3 stations of 5 minutes each followed by a 10 minute water rinse on an automatic stainer. I've never worked with Mayers as the primary hematoxylin. Can Mayers be used with a bluing agent instead of the long water rince? Longest darn H&E I've ever had to wait on!!!! --------------------------------- Ready for the edge of your seat? Check out tonight's top picks on Yahoo! TV. From dcojita <@t> tampabay.rr.com Fri Jul 27 21:34:07 2007 From: dcojita <@t> tampabay.rr.com (dcojita@tampabay.rr.com) Date: Fri Jul 27 21:36:40 2007 Subject: [Histonet] Histology position in sunny Florida (Tampa) In-Reply-To: <7E3ACD48BA6E26408F3188FBF08693F7AC920B@titansbs1.corp.titanmed.com> Message-ID: Our Independent laboratory has a position open for a histologist. Early shift, embedding, cutting, special stains, etc. It is a beautiful state-of-the art facility with new equipment. The lab is located 5 minutes from the Tampa airport. Our focus is on the patient and producing quality slides. We are CAP accredited. We have great benefits and salary. If anyone is interested please forward your resume to cojitadi@reliancepathology.com or call 813-490-7215 and ask for Diane. Thanks! From mbmphoto <@t> gmail.com Sat Jul 28 01:14:17 2007 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Sat Jul 28 01:12:43 2007 Subject: [Histonet] need protocol for neurturin (NTN) IHC labeling for primate sections Message-ID: Hello, Early this week, I posted this message, however I received no responses. So, I'm sending the message again in the hopes that someone out there is able to help me. I'm in need of a protocol using neurturin (NTN) IHC antibody labeling on primate 40um cryostat free-floating sections that have been aldehyde fixed. We have tried NTN antibodies from both Chemicon & RD Systems without success. I would greatly appreciate any assistance. Best Regards Maria Bartola Mejia UCSF Department of Neurosurgery SA CA 94103 From mickie25 <@t> netzero.net Sat Jul 28 07:16:39 2007 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Sat Jul 28 07:17:34 2007 Subject: [Histonet] mayers bluing In-Reply-To: <464443.40361.qm@web38210.mail.mud.yahoo.com> References: <464443.40361.qm@web38210.mail.mud.yahoo.com> Message-ID: Hi Steve, Mayer's is a lot like Gill I. Yes you can use a bluing solution like ammonia water or Scott's tap water very well and cut you time down considerably. Send me your protocol and I will review if you like. Good Luck Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven Coakley Sent: Friday, July 27, 2007 6:31 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] mayers bluing I just started working in a Derm. lab that uses Mayers, 3 stations of 5 minutes each followed by a 10 minute water rinse on an automatic stainer. I've never worked with Mayers as the primary hematoxylin. Can Mayers be used with a bluing agent instead of the long water rince? Longest darn H&E I've ever had to wait on!!!! --------------------------------- Ready for the edge of your seat? Check out tonight's top picks on Yahoo! TV. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sat Jul 28 07:52:44 2007 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Jul 28 07:52:49 2007 Subject: [Histonet] mayers bluing In-Reply-To: <464443.40361.qm@web38210.mail.mud.yahoo.com> Message-ID: <703892.27435.qm@web61221.mail.yahoo.com> Mayer's, as you know, is a progressive hematoxylin, meaning that you do not have to differentiate it; you just need to determine an adequate staining time for it to stain. After that washing has 2 purposes: eliminate excess hematoxylin and turn it blue (hematoxylin is a pH indicator). To you short question, a short answer: YES, after washing the excess, you can "blue" it (let it act as the indicator it is in an alkaline or basic solution) in what are kown as "blueing solutions". Ren? J. Steven Coakley wrote: I just started working in a Derm. lab that uses Mayers, 3 stations of 5 minutes each followed by a 10 minute water rinse on an automatic stainer. I've never worked with Mayers as the primary hematoxylin. Can Mayers be used with a bluing agent instead of the long water rince? Longest darn H&E I've ever had to wait on!!!! --------------------------------- Ready for the edge of your seat? Check out tonight's top picks on Yahoo! TV. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better Heartthrob. Get better relationship answers from someone who knows. Yahoo! Answers - Check it out. From AnthonyH <@t> chw.edu.au Sun Jul 29 17:57:28 2007 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Jul 29 17:57:59 2007 Subject: [Histonet] Fat issues Message-ID: Mickie, We used your technique many times and it works a treat. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mickie Johnson Sent: Saturday, 28 July 2007 11:12 AM To: 'Joe Nocito'; AGrobe2555@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Fat issues Hello Albert, Joe and all you dedicated Histonetters! Happy Friday! I wrote an article in the Histo-Logic about 4 years ago, (It is in the archives) about a very useful technique for coping with this problem. Here it is in a nut shell. 1. Melt the block down. Save the cassette 2. Blot off all wax. Don't press as this distorts the tissue 3. Put the specimen back in its original cassette and put a lid on. 4. Put it in the with the current days run starting in formalin. 5. Process as usual. 6. embed as usual. You will find that the under processed part is now processed and the part that was ok to begin with will not be harmed by the reprocessing. That part was protected by the paraffin that had infiltrated it originally. Good Luck. Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Friday, July 27, 2007 9:20 AM To: AGrobe2555@aol.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Fat issues Albert, could be the fat tissues didn't completely process. I read about a technique a few years ago (I can't remember the author,sorry) where you melt the block done, put the tissue between several pieces of paper towels and gently press on the tissue to expel any remaining xylene and re-embed. I did this for a couple of colon cases and it worked for me. It beats reprocessing the entire block. JTT ----- Original Message ----- From: To: Sent: Friday, July 27, 2007 10:31 AM Subject: [Histonet] Fat issues > Hello All, > I have some vascular graft tissues that we have paraffin embedded and > some of the tissues have extra-vascular fat. The practice sample I > sectioned this morning seems to section well (other than the suture > issues), however the > fat > "blows up" as soon as I place the sections into the flotation bath. > Is there some way to minimize this? I will try cooling down the > water bath temperature, as it may have been a bit warm. > > Any suggestions are welcome. > Thanks, > Albert > > Albert C. Grobe, PhD > International Heart Institute of Montana Foundation > Tissue Engineering Lab, Saint Patrick Hospital > > > > > > ************************************** Get a sneak peek of the all-new > AOL > at > http://discover.aol.com/memed/aolcom30tour > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From mbmphoto <@t> gmail.com Mon Jul 30 00:13:37 2007 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Mon Jul 30 00:12:02 2007 Subject: [Histonet] test Message-ID: <4D8F27F8-30D1-42BA-BBE6-4DA5F61B32A8@gmail.com> Apparently I can receive histonet messages, however I've been unable to post any messages. Can someone help? Maria Bartola Mejia UCSF Department of Neurosurgery SF CA. 94103 From talulahgosh <@t> gmail.com Mon Jul 30 01:15:30 2007 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Mon Jul 30 01:15:34 2007 Subject: [Histonet] mouse spinal cord atlas In-Reply-To: References: Message-ID: Have you tried looking through google? I know it sounds silly, but many people have their data posted online, whether through journals or their institute. Also, I would consult someone who publishes frequently on mouse spinal cord development. For example, my PI knows that the lumbar region in mice corresponds to certain anatomical features--this is through extensive research on pubmed, not any book she has. I can also suggest two names for research online or correspondence: Mario Capecchi and Tom Jessell. There are many other labs dealing with mouse spinal cord development, but these come to mind first. PLEASE note that I'm saying this personally, not through my lab or my institution. Emily -- these things happen, you know, you go for a walk in the park one day and wheelchair ninjas and nazis and pots-and-pans robots show up to kill you and dinosaurs show up to eat the remains. From garth <@t> apollosci.co.za Mon Jul 30 01:54:09 2007 From: garth <@t> apollosci.co.za (Garth Jerome) Date: Mon Jul 30 01:54:51 2007 Subject: [Histonet] Decal In-Reply-To: <6.0.0.22.1.20070728085830.01b40e58@gemini.msu.montana.edu> Message-ID: <000601c7d276$6f791a90$7700a8c0@jhb.apollosci.co.za> Hello Gayle My mistake, apologies! Garth Jerome Apollo Scientific cc Telephone : 27-11-466 7666 Fascimile : 27-11-466 7672 Email : garth@apollosci.co.za -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: 28 July 2007 05:00 PM To: Garth Jerome Subject: RE: [Histonet] Decal I was not the one asking the original question, I replied to Amy Self and asked her what species was being decalcified. She replied human bone. Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 At 12:01 AM 7/27/2007, you wrote: >Hi Gayle >You didn't mention the size or species of bones. >We are currently using 5% formic acid for decal of human bone. >We are using a formic acid / HCL combo for animal bones. > >Regards > >Garth Jerome >Apollo Scientific cc >Telephone : 27-11-466 7666 >Fascimile : 27-11-466 7672 >Email : garth@apollosci.co.za > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis >Sent: 26 July 2007 05:47 PM >To: Amy Self; Histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] Decal > > From what species? > >At 06:06 AM 7/26/2007, you wrote: > > > > Good Morning Histonetters, > > > > I was wandering what protocols/procedures where being used for > > decal of bones? We only decal knee bones and femurs. Here lately we > > have been having a time.... It seems like nothing we try works. > > > > Thanks in advance, Amy > > > > > > Amy Self > > Georgetown Hospital Systems > >Gayle Callis >MT,HT,HTL(ASCP) >Research Histopathology Supervisor >Veterinary Molecular Biology >Montana State University - Bozeman >PO Box 173610 >Bozeman MT 59717-3610 > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From louise.renton <@t> gmail.com Mon Jul 30 02:58:16 2007 From: louise.renton <@t> gmail.com (louise renton) Date: Mon Jul 30 02:58:58 2007 Subject: [Histonet] interesting pictures In-Reply-To: <766412.31625.qm@web37005.mail.mud.yahoo.com> References: <766412.31625.qm@web37005.mail.mud.yahoo.com> Message-ID: Looking at the pictures of the Russian lab one thing struck me forcibly. They have a WINDOW, with pot plants, and sunshine and green trees outside. For all the mod cons in my lab, we work in a windowless, artificially lit space, and the only windows (in the secretary's office) look out onto the concrete side of another building! Sometimes, its the little things that count! On 7/27/07, Constance McManus wrote: > > gudrun > thank you for sharing these photos! One of the PAs in our dept lived in > Latvia for 2 years and he has interesting stories about the decrepit and > poor condition the infrastructural elements are in the former USSR > countries. It's not just medicine, it's also construction, manufacturing, > agriculture, etc. And, from what I understand, it's not getting better. > > Sadly, my first impulse was to laugh at these pictures. I say "sadly" > because one should not mock another's misfortune. The more I looked at > those picutres, then glanced around the lab I work in, I began to feel a > sense of gratitude for all that I generally take fore granted. My heart is > filled with compassion for those people over there and I salute all of you > who are donating your time, talents & expertise, money, your lives to help > the people from these countries make a better life. If the day ever comes > that I have the means to do something like that, I would hop like a frog on > a hot pan to do it. > > thanks for opening my eyes to what I have!!! > > Connie McManus, HT > University of Utah > School of Medicine > Dept. of Dermatology > > > > > ----- Original Message ---- > From: Nancy Lemke > To: gu.lang@gmx.at; histonet@lists.utsouthwestern.edu > Sent: Thursday, July 26, 2007 10:28:44 AM > Subject: Re: [Histonet] interesting pictures > > I think that everyone who feels underequipped should look at these > fascinating photographs. It is like turning the clock back 60+ > years. Thank you so much for putting them on Histonet. > > Nancy Lemke > Research Coordinator > Hermelin Brain Tumor Center > Henry Ford Hospital > Detroit > -----Original message----- > From: "Gudrun Lang" gu.lang@gmx.at > Date: Thu, 26 Jul 2007 12:20:11 -0400 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] interesting pictures > > > Hi all, > > > > I found these interesting pictures of a Russian pathology in the web. > > > > http://www.freewebs.com/ruspat1/biospylaboratory.htm > > > > > > > > > > > > Gudrun > > > > > > > > ============================================================================== > CONFIDENTIALITY NOTICE: This email contains information from the sender > that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise > protected from disclosure. This email is intended for use only by the person > or entity to whom it is addressed. If you are not the intended recipient, > any use, disclosure, copying, distribution, printing, or any action taken in > reliance on the contents of this email, is strictly prohibited. If you > received this email in error, please contact the sending party by reply > email, delete the email from your computer system and shred any paper > copies. > > Note to Patients: There are a number of risks you should consider before > using e-mail to communicate with us. See our Privacy Policy and Henry Ford > My Health at www.henryford.com for more detailed information. If you do > not believe that our policy gives you the privacy and security protection > you need, do not send e-mail or Internet communications to us. > > > ============================================================================== > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > ____________________________________________________________________________________ > Building a website is a piece of cake. Yahoo! Small Business gives you all > the tools to get online. > http://smallbusiness.yahoo.com/webhosting > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From Janet.Bonner <@t> FLHosp.org Mon Jul 30 06:45:26 2007 From: Janet.Bonner <@t> FLHosp.org (Bonner, Janet) Date: Mon Jul 30 06:46:39 2007 Subject: [Histonet] interesting pictures References: <766412.31625.qm@web37005.mail.mud.yahoo.com> Message-ID: <5F31F38C96781A4FBE3196EBC22D47802E10A4@fhosxchmb006.ADVENTISTCORP.NET> AND....They can open that window and get a fresh air (albeit possibly warm/hot) cross breeze. I can remember working in the night in NY because there was no air conditioning and we actually had areas that looked like that when I started in Histology!!!! By 11AM, we were actually so high on the fumes, the Pathologists had to send us home because we were so noisy (laughing at everything.) -What a way to go, that was 35 years ago and I'm still alive and OK! (well, still alive anyway). These pictures from Russia made me count my blessings, now I don't think I could stand the fumes and exposure!! Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of louise renton Sent: Mon 7/30/2007 3:58 AM To: Constance McManus Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] interesting pictures Looking at the pictures of the Russian lab one thing struck me forcibly. They have a WINDOW, with pot plants, and sunshine and green trees outside. For all the mod cons in my lab, we work in a windowless, artificially lit space, and the only windows (in the secretary's office) look out onto the concrete side of another building! Sometimes, its the little things that count! On 7/27/07, Constance McManus wrote: > > gudrun > thank you for sharing these photos! One of the PAs in our dept lived in > Latvia for 2 years and he has interesting stories about the decrepit and > poor condition the infrastructural elements are in the former USSR > countries. It's not just medicine, it's also construction, manufacturing, > agriculture, etc. And, from what I understand, it's not getting better. > > Sadly, my first impulse was to laugh at these pictures. I say "sadly" > because one should not mock another's misfortune. The more I looked at > those picutres, then glanced around the lab I work in, I began to feel a > sense of gratitude for all that I generally take fore granted. My heart is > filled with compassion for those people over there and I salute all of you > who are donating your time, talents & expertise, money, your lives to help > the people from these countries make a better life. If the day ever comes > that I have the means to do something like that, I would hop like a frog on > a hot pan to do it. > > thanks for opening my eyes to what I have!!! > > Connie McManus, HT > University of Utah > School of Medicine > Dept. of Dermatology > > > > > ----- Original Message ---- > From: Nancy Lemke > To: gu.lang@gmx.at; histonet@lists.utsouthwestern.edu > Sent: Thursday, July 26, 2007 10:28:44 AM > Subject: Re: [Histonet] interesting pictures > > I think that everyone who feels underequipped should look at these > fascinating photographs. It is like turning the clock back 60+ > years. Thank you so much for putting them on Histonet. > > Nancy Lemke > Research Coordinator > Hermelin Brain Tumor Center > Henry Ford Hospital > Detroit > -----Original message----- > From: "Gudrun Lang" gu.lang@gmx.at > Date: Thu, 26 Jul 2007 12:20:11 -0400 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] interesting pictures > > > Hi all, > > > > I found these interesting pictures of a Russian pathology in the web. > > > > http://www.freewebs.com/ruspat1/biospylaboratory.htm > > > > > > > > > > > > Gudrun > > > > > > > > ============================================================================== > CONFIDENTIALITY NOTICE: This email contains information from the sender > that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise > protected from disclosure. This email is intended for use only by the person > or entity to whom it is addressed. If you are not the intended recipient, > any use, disclosure, copying, distribution, printing, or any action taken in > reliance on the contents of this email, is strictly prohibited. If you > received this email in error, please contact the sending party by reply > email, delete the email from your computer system and shred any paper > copies. > > Note to Patients: There are a number of risks you should consider before > using e-mail to communicate with us. See our Privacy Policy and Henry Ford > My Health at www.henryford.com for more detailed information. If you do > not believe that our policy gives you the privacy and security protection > you need, do not send e-mail or Internet communications to us. > > > ============================================================================== > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > ____________________________________________________________________________________ > Building a website is a piece of cake. Yahoo! Small Business gives you all > the tools to get online. > http://smallbusiness.yahoo.com/webhosting > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From relia1 <@t> earthlink.net Mon Jul 30 07:21:38 2007 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Jul 30 07:22:39 2007 Subject: [Histonet] RELIA's Histology Careers Bulletin 7-30-07 Message-ID: Hi Histonetters, I hope you are having a great summer. I just wanted to drop you a line and tell you about my current histology openings. All the positions I represent are permanent positions with great companies who offer excellent compensation, benefits and relocation/sign on bonuses. Wherever you want to go, wherever you want to stay if you are looking for a new opportunity I can help. I offer over 20 years of recruiting experience and a recruiting practice solely dedicated to permanent placement in the Histology profession. In addition to representing you to the positions you are interested in with my clients I offer to you FREE of Charge: ? Assistance with updating or creating your resume ? Tips on interviewing ?Encouragement and assistance during the course of your job search ?Complete Confidentiality ( I will only represent you to jobs you tell me you are interested in looking into) Here is a list of my current openings: HISTOLOGY SUPERVISORS/MANAGERS Histology Manager ? Chicago, IL Histology Manager ? Central CA Histology Manager - MA New Grads Welcome to Apply! HISTOTECHNICIANS/HISTOTECHNOLOGISTS: Histo Tech - Naples, FL Dermpath Histo Tech ? Orlando, FL Histo Tech ? Virginia, near Virginia Beach Research Histo Tech ? Boston, MA Histo Tech South ? South TX Inside Tech Support Rep - WI Histo Tech ? Southern OK Histo Tech- Northern IL Histo Tech ? San Francisco Bay Area, CA Jr. Mohs Tech ? Los Angeles, CA Histo Tech ? WA Histo Tech ? Pittsburgh, PA Histo Tech ? near Columbus, OH If you or any of your friends would like more information on any of these positions. Or if you would like to discuss opportunities in other areas or future job searches please contact me. I would be more than happy to assist you. You can reach me at 866-607-3542 or relia1@earthlink.net I work with facilities nationwide and I will keep your resume confidential. I will only represent you to jobs you tell me you are interested in looking into. Remember It never hurts to keep an eye open even if you are happy in your present job. Thank you, Pam ? 866-607-3542 (866-60RELIA) Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net From cmiller <@t> physlab.com Mon Jul 30 07:11:55 2007 From: cmiller <@t> physlab.com (Cheri Miller) Date: Mon Jul 30 07:33:38 2007 Subject: [Histonet] hemoglobin In-Reply-To: <46A76A65.6010908@tufts.edu> References: <46A76A65.6010908@tufts.edu> Message-ID: <00dc01c7d2a2$d5ee07f0$3402a8c0@plab.local> This may be a dumb question, But why would the inspector insist you do a hemoglobin stain if it's not part of your testing menu?? What entity is inspecting your lab? Just curious, Cheri Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Derek Papalegis Sent: Wednesday, July 25, 2007 10:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] hemoglobin Hi All, An investigator has asked me to stain some sections for hemoglobin. I have already provided him with an iron stain and he wants to go further with it. Can anyone recommend a stain specifically for hemoglobin? I have found some for hemosiderin but I am unsure as to if they will be sufficient. If someone could let me know what stain they use and what the procedure is I would greatly appreciate it. Thanks! -Derek -- Derek Papalegis HT (ASCP) Histotechnician Division of Laboratory Animal Medicine Tufts University 136 Harrison Avenue Boston, MA 02111 phone: 617 636-2971 fax: 617 636-8354 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From sonya.martin <@t> soton.ac.uk Mon Jul 30 08:55:13 2007 From: sonya.martin <@t> soton.ac.uk (Martin S.) Date: Mon Jul 30 08:58:49 2007 Subject: [Histonet] Cytospin - misshapen cells Message-ID: <71437982F5B13A4D9A5B2669BDB89EE40F504087@ISS-CL-EX-V1.soton.ac.uk> Hi All, I'm having some trouble with cytospins - the cells/nuclei look very misshapen after spinning. I have used a number of different cell lines and have the same problem with them all. I have tried fixing the cells before spinning with 4% PFA in PBS (for this I centrifge the cells, resuspend in PBS, centrifuge, resuspend in PFA for 10min on ice, centrifuge, resuspend in PBS and cytospin). Unfixed cells were cytopsun in media. The cytospin is set to 500rpm for 5min with low acceleration. After spinning I let them air dry (~5min), wash PBS, fix 4% PFA (if not post-fixed), wash PBS and continue with staining. When staining the nuceli just with Dapi I get some very strange looking nuclei, please see some pics on www.histonet.org the file is called Cytospin Dapi.jpg. Is this normal for cytospins? Any ideas? Thanks Sonya From AGrobe2555 <@t> aol.com Mon Jul 30 09:17:39 2007 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Mon Jul 30 09:55:25 2007 Subject: [Histonet] RE: Fat Issues Message-ID: My thanks to all who helped me out with this issue. I received many helpful suggestions and may implement them if my tissues prove to be problematic. I appreciate all the help! Albert Albert C. Grobe, PhD International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital ************************************** Get a sneak peek of the all-new AOL at http://discover.aol.com/memed/aolcom30tour From sonya.martin <@t> soton.ac.uk Mon Jul 30 09:00:53 2007 From: sonya.martin <@t> soton.ac.uk (Martin S.) Date: Mon Jul 30 10:17:25 2007 Subject: [Histonet] Recycling lab equipment? Message-ID: <71437982F5B13A4D9A5B2669BDB89EE40F5040AF@ISS-CL-EX-V1.soton.ac.uk> In light of the recent pictures of the under-equipped Russian labs, does anyone know of any organisations (in the UK) which recycle lab equipment? We have a couple of old microscopes and loads of slide-staining racks etc which are gathering dust. The microscopes are very old (1950's possibly!) and not great optically but with a bit of TLC they may be of use to someone. Someone suggested trying to sell them on ebay as antiques!! Sonya From jmahoney <@t> alegent.org Mon Jul 30 07:45:40 2007 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Mon Jul 30 10:32:07 2007 Subject: [Histonet] hemoglobin In-Reply-To: <00dc01c7d2a2$d5ee07f0$3402a8c0@plab.local> References: <46A76A65.6010908@tufts.edu> <00dc01c7d2a2$d5ee07f0$3402a8c0@plab.local> Message-ID: <46AD97240200003C00015377@gwia.alegent.org> I think Derek is talking about a request to do a stain by an investigator, not a lab inspector. Jan Omaha >>> "Cheri Miller" 07/30/2007 7:11 AM >>> This may be a dumb question, But why would the inspector insist you do a hemoglobin stain if it's not part of your testing menu?? What entity is inspecting your lab? Just curious, Cheri Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Derek Papalegis Sent: Wednesday, July 25, 2007 10:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] hemoglobin Hi All, An investigator has asked me to stain some sections for hemoglobin. I have already provided him with an iron stain and he wants to go further with it. Can anyone recommend a stain specifically for hemoglobin? I have found some for hemosiderin but I am unsure as to if they will be sufficient. If someone could let me know what stain they use and what the procedure is I would greatly appreciate it. Thanks! -Derek -- Derek Papalegis HT (ASCP) Histotechnician Division of Laboratory Animal Medicine Tufts University 136 Harrison Avenue Boston, MA 02111 phone: 617 636-2971 fax: 617 636-8354 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sonya.martin <@t> soton.ac.uk Mon Jul 30 09:56:51 2007 From: sonya.martin <@t> soton.ac.uk (Martin S.) Date: Mon Jul 30 10:38:19 2007 Subject: [Histonet] Cytospin - misshapen cells In-Reply-To: References: <71437982F5B13A4D9A5B2669BDB89EE40F504087@ISS-CL-EX-V1.soton.ac.uk> Message-ID: <71437982F5B13A4D9A5B2669BDB89EE40F50419B@ISS-CL-EX-V1.soton.ac.uk> Yes, I wondered if it was just a cytospin problem - although other people seem to get great results with cytospins! They just don't seem to be able to let the rest of us in on the secret! I wanted to use the cells as a positive control so they're not that important (I already have some positively stained cells (nice adherent cells!), a negative control and an isotype control so........) For future reference whats your protocol for embedding in agarose and when you say to treat like a tissue block do you mean a paraffin embedded tissue block or frozen? - I have previously embedded cell pellets in sucrose for ultrathin cryosectioning but this seems like a lot of hassle.......... Thanks Sonya -----Original Message----- From: Edwards, R.E. [mailto:ree3@leicester.ac.uk] Sent: 30 July 2007 15:28 To: Martin S. Subject: RE: [Histonet] Cytospin - misshapen cells Many years ago I tried cytospinning various types of cells, cytospinning introduces so many of its own variables, such as rpm, time of spinning and cell concentration, which markedly affected the outcome of the quality of the cell prep, and the morphology of the cell depending on its position on within the ring that I abandoned it in favour of just smearing the cells onto coated slides or made cell pellets embedded them in agarose and treated it like a tissue block... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin S. Sent: 30 July 2007 14:55 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cytospin - misshapen cells Hi All, I'm having some trouble with cytospins - the cells/nuclei look very misshapen after spinning. I have used a number of different cell lines and have the same problem with them all. I have tried fixing the cells before spinning with 4% PFA in PBS (for this I centrifge the cells, resuspend in PBS, centrifuge, resuspend in PFA for 10min on ice, centrifuge, resuspend in PBS and cytospin). Unfixed cells were cytopsun in media. The cytospin is set to 500rpm for 5min with low acceleration. After spinning I let them air dry (~5min), wash PBS, fix 4% PFA (if not post-fixed), wash PBS and continue with staining. When staining the nuceli just with Dapi I get some very strange looking nuclei, please see some pics on www.histonet.org the file is called Cytospin Dapi.jpg. Is this normal for cytospins? Any ideas? Thanks Sonya _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ASelf <@t> gmhsc.com Mon Jul 30 11:06:45 2007 From: ASelf <@t> gmhsc.com (Amy Self) Date: Mon Jul 30 11:17:36 2007 Subject: [Histonet] Excelsior Tissue Processor Message-ID: I am looking for information from anyone that has an Excelsior tissue processor from Shandon. I'd like to know the pros and cons and how it compares to the VIP-5. Thanks in advance for your help, Amy Amy Self Georgetown Hospital System Georgetown, SC NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From ralphpu <@t> alleninstitute.org Mon Jul 30 11:34:50 2007 From: ralphpu <@t> alleninstitute.org (Ralph Puchalski) Date: Mon Jul 30 11:47:58 2007 Subject: [Histonet] mouse spinal cord atlas In-Reply-To: References: Message-ID: Thank you for the suggestions. Yes, I have checked Google and Tom. I will look into Mario's work. We are developing a gene expression atlas. A histological atlas is being created now by others, which will help a great deal. Ralph Ralph B. Puchalski, Ph.D. Research Alliance Manager Allen Institute for Brain Science 551 N. 34th Street, Suite 200 Seattle, WA 98103 ralphpu@alleninstitute.org Tel: 206-548-7041 Fax: 206-548-7083 www.brain-map.org, www.alleninstitute.org -----Original Message----- From: Emily Sours [mailto:talulahgosh@gmail.com] Sent: Sunday, July 29, 2007 11:16 PM To: Ralph Puchalski; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] mouse spinal cord atlas Have you tried looking through google? I know it sounds silly, but many people have their data posted online, whether through journals or their institute. Also, I would consult someone who publishes frequently on mouse spinal cord development. For example, my PI knows that the lumbar region in mice corresponds to certain anatomical features--this is through extensive research on pubmed, not any book she has. I can also suggest two names for research online or correspondence: Mario Capecchi and Tom Jessell. There are many other labs dealing with mouse spinal cord development, but these come to mind first. PLEASE note that I'm saying this personally, not through my lab or my institution. Emily -- these things happen, you know, you go for a walk in the park one day and wheelchair ninjas and nazis and pots-and-pans robots show up to kill you and dinosaurs show up to eat the remains. From ralphpu <@t> alleninstitute.org Mon Jul 30 11:49:44 2007 From: ralphpu <@t> alleninstitute.org (Ralph Puchalski) Date: Mon Jul 30 11:50:51 2007 Subject: [Histonet] mouse spinal cord atlas In-Reply-To: References: Message-ID: Dear John, Thank you for your detailed reply. I filmed a person removing an intact cord without the roots in ~10 min, so maybe instruments are a little better these days. I also just learned that a mouse spinal cord histological atlas is being created, and should be completed later this year. I am getting help from the scientific lead. Ralph ________________________________ From: John Kiernan [mailto:jkiernan@uwo.ca] Sent: Friday, July 27, 2007 9:45 PM To: Ralph Puchalski Subject: Re: [Histonet] mouse spinal cord atlas Dear Ralph P., You'll need a mouse anatomy book. You can't transfer cord-vertebra correlations from one species to another. There's a blue rat anatomy book that provides a diagram similar to the familiar ones seen in textbooks of human anatomy and neuroanatomy. [I have the rat book, but not at home, and forget the author; you couldn't assume the levels are the same in rat and mouse, but it might give approximate guidance. There could also be variation among different strains of lab mouse.] The only way to be certain is to dissect the animal from the dorsal aspect, which is a 2-day job requiring much patience and the right instruments. That's what I was told 20 years ago by someone who regularly removed mouse spinal cords. I've never tried! I have removed rat spinal cords the easy way (Remove head above vertebra C1, clip across the lumbo-sacral junction and inject saline into the vertebral canal; catch the ejected cord in a dish of saline or fixative). The cervical and thoracic nerve roots are lost, but many lumbosacral and caudal roots come out still attached to the cord. This can probably be done with the mouse, and you could identify lower segments of the cord by counting roots upward from the conus medullaris, assuming that you know the numbers of caudal, sacral and lumbar nerves in the mouse. This method of removing the spinal cord won't allow accurate collection of cervical and thoracic segments. Bear in mind that the levels of vertebral bodies are different from the levels of the tips of their dorsal spines. The sacral segments of the human cord (with which I have quite a lot of experience) are very short: all five occupy about an inch, which is level with the T12-L1 disk and about half of the vertebral bodies T12 and L1 above and below. Anatomical details other than skeletal level are used for obtaining specimens of specific sacral and lower lumbar segments of the human spinal cord. If you need exact cord segments you will probably have to do the laborious dissection with instruments that can remove mouse erector spinae muscles and vertebral laminae. John Kiernan Anatomy, UWO London, Canada -- ----- Original Message ----- From: Ralph Puchalski Date: Friday, July 27, 2007 20:00 Subject: [Histonet] mouse spinal cord atlas To: histonet@lists.utsouthwestern.edu > I am looking for an image or sketch of the mouse cord and column > showingthe relationship of the cord to the column, and possibly > to the spinal > nerves. I have a textbook sketch for human, which shows > for example > that L1 (the first of the lumbar segment) of the cord > corresponds in > space (is adjacent) to T11 (the eleventh of the thoracic > segment) of the > ventral column. Does anyone have or know where I could > find such a > picture? The atlases I have seen don't have the entire > cord or column, > just tissue sections cut periodically throughout the cord. > > Ralph > > > > Ralph B. Puchalski, Ph.D. > > Research Alliance Manager > > Allen Institute for Brain Science > > 551 N. 34th Street, Suite 200 > > Seattle, WA 98103 > > > > ralphpu@alleninstitute.org > > Tel: 206-548-7041 Fax: 206-548-7083 > > www.brain-map.org , > www.alleninstitute.org > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From cmiller <@t> physlab.com Mon Jul 30 08:39:40 2007 From: cmiller <@t> physlab.com (Cheri Miller) Date: Mon Jul 30 12:25:39 2007 Subject: [Histonet] hemoglobin In-Reply-To: <46AD97240200003C00015377@gwia.alegent.org> References: <46A76A65.6010908@tufts.edu> <00dc01c7d2a2$d5ee07f0$3402a8c0@plab.local> <46AD97240200003C00015377@gwia.alegent.org> Message-ID: <00e301c7d2af$17dfa130$3402a8c0@plab.local> That makes more sense....Investigator = Pathologist ?? You had me worried for a few. Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: Janice Mahoney [mailto:jmahoney@alegent.org] Sent: Monday, July 30, 2007 7:46 AM To: histonet@lists.utsouthwestern.edu; Cheri Miller; 'Derek Papalegis' Subject: RE: [Histonet] hemoglobin I think Derek is talking about a request to do a stain by an investigator, not a lab inspector. Jan Omaha >>> "Cheri Miller" 07/30/2007 7:11 AM >>> This may be a dumb question, But why would the inspector insist you do a hemoglobin stain if it's not part of your testing menu?? What entity is inspecting your lab? Just curious, Cheri Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Derek Papalegis Sent: Wednesday, July 25, 2007 10:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] hemoglobin Hi All, An investigator has asked me to stain some sections for hemoglobin. I have already provided him with an iron stain and he wants to go further with it. Can anyone recommend a stain specifically for hemoglobin? I have found some for hemosiderin but I am unsure as to if they will be sufficient. If someone could let me know what stain they use and what the procedure is I would greatly appreciate it. Thanks! -Derek -- Derek Papalegis HT (ASCP) Histotechnician Division of Laboratory Animal Medicine Tufts University 136 Harrison Avenue Boston, MA 02111 phone: 617 636-2971 fax: 617 636-8354 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. 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From cmiller <@t> physlab.com Mon Jul 30 07:59:58 2007 From: cmiller <@t> physlab.com (Cheri Miller) Date: Mon Jul 30 12:25:49 2007 Subject: [Histonet] Fat issues In-Reply-To: References: <002e01c7d069$fe979940$d49eae18@yourxhtr8hvc4p> Message-ID: <00e101c7d2a9$8a3a96f0$3402a8c0@plab.local> I melt down the block, recap and run it thru my clean cycle on the VIP and then place back in formalin and run with the next days cases. It works beautifully. Cheri Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mickie Johnson Sent: Friday, July 27, 2007 8:12 PM To: 'Joe Nocito'; AGrobe2555@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Fat issues Hello Albert, Joe and all you dedicated Histonetters! Happy Friday! I wrote an article in the Histo-Logic about 4 years ago, (It is in the archives) about a very useful technique for coping with this problem. Here it is in a nut shell. 1. Melt the block down. Save the cassette 2. Blot off all wax. Don't press as this distorts the tissue 3. Put the specimen back in its original cassette and put a lid on. 4. Put it in the with the current days run starting in formalin. 5. Process as usual. 6. embed as usual. You will find that the under processed part is now processed and the part that was ok to begin with will not be harmed by the reprocessing. That part was protected by the paraffin that had infiltrated it originally. Good Luck. Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Friday, July 27, 2007 9:20 AM To: AGrobe2555@aol.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Fat issues Albert, could be the fat tissues didn't completely process. I read about a technique a few years ago (I can't remember the author,sorry) where you melt the block done, put the tissue between several pieces of paper towels and gently press on the tissue to expel any remaining xylene and re-embed. I did this for a couple of colon cases and it worked for me. It beats reprocessing the entire block. JTT ----- Original Message ----- From: To: Sent: Friday, July 27, 2007 10:31 AM Subject: [Histonet] Fat issues > Hello All, > I have some vascular graft tissues that we have paraffin embedded and some > of the tissues have extra-vascular fat. The practice sample I sectioned > this > morning seems to section well (other than the suture issues), however the > fat > "blows up" as soon as I place the sections into the flotation bath. Is > there some way to minimize this? I will try cooling down the water bath > temperature, as it may have been a bit warm. > > Any suggestions are welcome. > Thanks, > Albert > > Albert C. Grobe, PhD > International Heart Institute of Montana Foundation > Tissue Engineering Lab, Saint Patrick Hospital > > > > > > ************************************** Get a sneak peek of the all-new AOL > at > http://discover.aol.com/memed/aolcom30tour > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From cmiller <@t> physlab.com Mon Jul 30 07:57:11 2007 From: cmiller <@t> physlab.com (Cheri Miller) Date: Mon Jul 30 12:26:01 2007 Subject: [Histonet] interesting pictures In-Reply-To: <766412.31625.qm@web37005.mail.mud.yahoo.com> References: <766412.31625.qm@web37005.mail.mud.yahoo.com> Message-ID: <00e001c7d2a9$27274720$3402a8c0@plab.local> Beautifully spoken! My sentiments exactly. I wont complain again for what I in the past have referred to as "garage sale rejects" AKA my equipment....Cheri Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Constance McManus Sent: Friday, July 27, 2007 12:41 PM To: Nancy Lemke; gu.lang@gmx.at; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] interesting pictures gudrun thank you for sharing these photos! One of the PAs in our dept lived in Latvia for 2 years and he has interesting stories about the decrepit and poor condition the infrastructural elements are in the former USSR countries. It's not just medicine, it's also construction, manufacturing, agriculture, etc. And, from what I understand, it's not getting better. Sadly, my first impulse was to laugh at these pictures. I say "sadly" because one should not mock another's misfortune. The more I looked at those picutres, then glanced around the lab I work in, I began to feel a sense of gratitude for all that I generally take fore granted. My heart is filled with compassion for those people over there and I salute all of you who are donating your time, talents & expertise, money, your lives to help the people from these countries make a better life. If the day ever comes that I have the means to do something like that, I would hop like a frog on a hot pan to do it. thanks for opening my eyes to what I have!!! Connie McManus, HT University of Utah School of Medicine Dept. of Dermatology ----- Original Message ---- From: Nancy Lemke To: gu.lang@gmx.at; histonet@lists.utsouthwestern.edu Sent: Thursday, July 26, 2007 10:28:44 AM Subject: Re: [Histonet] interesting pictures I think that everyone who feels underequipped should look at these fascinating photographs. It is like turning the clock back 60+ years. Thank you so much for putting them on Histonet. Nancy Lemke Research Coordinator Hermelin Brain Tumor Center Henry Ford Hospital Detroit -----Original message----- From: "Gudrun Lang" gu.lang@gmx.at Date: Thu, 26 Jul 2007 12:20:11 -0400 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] interesting pictures > Hi all, > > I found these interesting pictures of a Russian pathology in the web. > > http://www.freewebs.com/ruspat1/biospylaboratory.htm > > > > > > Gudrun > > ============================================================================ == CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. 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Small Business gives you all the tools to get online. http://smallbusiness.yahoo.com/webhosting _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From cmiller <@t> physlab.com Mon Jul 30 08:03:29 2007 From: cmiller <@t> physlab.com (Cheri Miller) Date: Mon Jul 30 12:26:10 2007 Subject: [Histonet] Fat issues References: <002e01c7d069$fe979940$d49eae18@yourxhtr8hvc4p> Message-ID: <00e201c7d2aa$0860bfa0$3402a8c0@plab.local> I meant to add that the x-tra time in 100% and xylene melts the fat away. Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: Cheri Miller [mailto:cmiller@physlab.com] Sent: Monday, July 30, 2007 8:00 AM To: 'mickie25@netzero.net'; 'Joe Nocito'; 'AGrobe2555@aol.com'; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Fat issues I melt down the block, recap and run it thru my clean cycle on the VIP and then place back in formalin and run with the next days cases. It works beautifully. Cheri Cheri Miller HT ASCP Histology Supervisor Physicians Laboratory Services, Inc. Omaha, NE 68117 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mickie Johnson Sent: Friday, July 27, 2007 8:12 PM To: 'Joe Nocito'; AGrobe2555@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Fat issues Hello Albert, Joe and all you dedicated Histonetters! Happy Friday! I wrote an article in the Histo-Logic about 4 years ago, (It is in the archives) about a very useful technique for coping with this problem. Here it is in a nut shell. 1. Melt the block down. Save the cassette 2. Blot off all wax. Don't press as this distorts the tissue 3. Put the specimen back in its original cassette and put a lid on. 4. Put it in the with the current days run starting in formalin. 5. Process as usual. 6. embed as usual. You will find that the under processed part is now processed and the part that was ok to begin with will not be harmed by the reprocessing. That part was protected by the paraffin that had infiltrated it originally. Good Luck. Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Friday, July 27, 2007 9:20 AM To: AGrobe2555@aol.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Fat issues Albert, could be the fat tissues didn't completely process. I read about a technique a few years ago (I can't remember the author,sorry) where you melt the block done, put the tissue between several pieces of paper towels and gently press on the tissue to expel any remaining xylene and re-embed. I did this for a couple of colon cases and it worked for me. It beats reprocessing the entire block. JTT ----- Original Message ----- From: To: Sent: Friday, July 27, 2007 10:31 AM Subject: [Histonet] Fat issues > Hello All, > I have some vascular graft tissues that we have paraffin embedded and some > of the tissues have extra-vascular fat. The practice sample I sectioned > this > morning seems to section well (other than the suture issues), however the > fat > "blows up" as soon as I place the sections into the flotation bath. Is > there some way to minimize this? I will try cooling down the water bath > temperature, as it may have been a bit warm. > > Any suggestions are welcome. > Thanks, > Albert > > Albert C. Grobe, PhD > International Heart Institute of Montana Foundation > Tissue Engineering Lab, Saint Patrick Hospital > > > > > > ************************************** Get a sneak peek of the all-new AOL > at > http://discover.aol.com/memed/aolcom30tour > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From gcallis <@t> montana.edu Mon Jul 30 12:03:59 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Jul 30 12:50:45 2007 Subject: [Histonet] Cytospin - misshapen cells In-Reply-To: <71437982F5B13A4D9A5B2669BDB89EE40F50419B@ISS-CL-EX-V1.soto n.ac.uk> References: <71437982F5B13A4D9A5B2669BDB89EE40F504087@ISS-CL-EX-V1.soton.ac.uk> <71437982F5B13A4D9A5B2669BDB89EE40F50419B@ISS-CL-EX-V1.soton.ac.uk> Message-ID: <6.0.0.22.1.20070730105036.01b329e8@gemini.msu.montana.edu> We prefer to do a cell block and then frozen sections instead of cytospins. No matter how careful, gentle and little spin time, we use, the seem to ball up like little "BB's", and cytoplasm is very difficult to see. We have done cell preparations where the cells relax and adhere to the slide surface, and that is far superior to having the cells smashed against the slide. This is more time consuming but it give better cellular morphology. This is with fresh, unfixed cells. For a cell block, take 1 to 3 x 107 cells, spin down and wash three times with PBS, then resuspend the last cell pellet in 1 ml OCT, immerse end of tube into liquid nitrogen to snap freeze. To remove the frozen cell block, warm end of tube slightly by holding it, then pop the side of the tube against side of cryostat chamber. The block will pop right out. IF you get too many cells, cut thinner sections - it can be done at 3 um. You can use the wider bottom microcentrifuge tubes that hold 2 ml for a slightl conical but broader block face, but the method works well with other tubes as well. A note: Chris van der Loos does this method also. Good luck Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 At 08:56 AM 7/30/2007, you wrote: >Yes, I wondered if it was just a cytospin problem - although other >people seem to get great results with cytospins! They just don't seem to >be able to let the rest of us in on the secret! >I wanted to use the cells as a positive control so they're not that >important (I already have some positively stained cells (nice adherent >cells!), a negative control and an isotype control so........) > >For future reference whats your protocol for embedding in agarose and >when you say to treat like a tissue block do you mean a paraffin >embedded tissue block or frozen? - I have previously embedded cell >pellets in sucrose for ultrathin cryosectioning but this seems like a >lot of hassle.......... > >Thanks >Sonya > >-----Original Message----- >From: Edwards, R.E. [mailto:ree3@leicester.ac.uk] >Sent: 30 July 2007 15:28 >To: Martin S. >Subject: RE: [Histonet] Cytospin - misshapen cells > >Many years ago I tried cytospinning various types of cells, >cytospinning introduces so many of its own variables, such as rpm, >time of spinning and cell concentration, which markedly affected >the outcome of the quality of the cell prep, and the morphology >of the cell depending on its position on within the ring that I >abandoned it in favour of just smearing the cells >onto coated slides or made cell pellets embedded them in >agarose and treated it like a tissue block... > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin >S. >Sent: 30 July 2007 14:55 >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Cytospin - misshapen cells > > >Hi All, > >I'm having some trouble with cytospins - the cells/nuclei look very >misshapen after spinning. I have used a number of different cell lines >and have the same problem with them all. I have tried fixing the cells >before spinning with 4% PFA in PBS (for this I centrifge the cells, >resuspend in PBS, centrifuge, resuspend in PFA for 10min on ice, >centrifuge, resuspend in PBS and cytospin). Unfixed cells were cytopsun >in media. The cytospin is set to 500rpm for 5min with low acceleration. >After spinning I let them air dry (~5min), wash PBS, fix 4% PFA (if not >post-fixed), wash PBS and continue with staining. > >When staining the nuceli just with Dapi I get some very strange looking >nuclei, please see some pics on www.histonet.org the file is called >Cytospin Dapi.jpg. > >Is this normal for cytospins? Any ideas? > >Thanks >Sonya > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Mon Jul 30 12:11:04 2007 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Mon Jul 30 13:06:10 2007 Subject: [Histonet] not histology related (well, almost) Message-ID: <002701c7d2cc$a11d45c0$d49eae18@yourxhtr8hvc4p> Last night I went back in time 30 years (or so). Went to a concert that had Peaches & Herb, Maxine Nightingale, The Village People and KC & The Sunshine Band. I have to tell you, we have been dancing the YMCA song wrong all these years. I was taught by the horses themselves. At the NSH banquet, I'm gonna have to give everyone a lesson (this ought to be enough to get y'all to Denver. I just have to go to Denver. JTT is giving us a dance lesson). That and 2 bucks ought to get you a cup of coffee. Stay tuned. JTT From wilson_c <@t> ricerca.com Mon Jul 30 14:28:31 2007 From: wilson_c <@t> ricerca.com (Wilson, Carol) Date: Mon Jul 30 15:15:52 2007 Subject: [Histonet] Histology duties in necropsy Message-ID: <9D443EB9D0270143B5AAF190CB1A58A305064E84@dogwood.ricerca.com> Hi All, Taking an informal survey of anyone involved in vet histology, primarily research. How many histotechs out there actually perform necropsy or have histology departments that oversee that area? Thanks, Carol Carol Wilson Team Leader - Associate Scientist III - Histopathology Ricerca Biosciences, LLC From judi.ford <@t> roche.com Mon Jul 30 16:08:33 2007 From: judi.ford <@t> roche.com (Ford, Judi) Date: Mon Jul 30 16:10:39 2007 Subject: [Histonet] stains for myocardial injury Message-ID: Hi All, My co-workers and I were wondering what people use for staining myocardial injuries. We are planning on trying both Masson's and Gomori's Trichrome, but were told that there is a Gomori's that is optimized for myocardial injuries. Has anyone heard of this method? I've done the Gomori's on frozen tissue but we are using formalin fixed paraffin embedded tissue. Thanks for any input. Judi Ford Roche Palo Alto Palo Alto, CA From azdudley <@t> hotmail.com Mon Jul 30 16:32:46 2007 From: azdudley <@t> hotmail.com (anita dudley) Date: Mon Jul 30 16:35:55 2007 Subject: [Histonet] breast Message-ID: I asked this question a few weeks ago and got 1 answer, what is everyone doing about the new cap requirements about fixing breast tissue no more that 48 hrs if a her2 is to be done? we do not work on the week end and friday that would be a problem if we had a breast. thanks for your input. anita _________________________________________________________________ See what you?re getting into?before you go there. http://newlivehotmail.com From algranth <@t> u.arizona.edu Mon Jul 30 16:47:09 2007 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Mon Jul 30 16:50:59 2007 Subject: [Histonet] stains for myocardial injury In-Reply-To: References: Message-ID: <6.2.3.4.1.20070730144421.01f42d00@algranth.inbox.email.arizona.edu> This is the stain I have done for one of the investigators here: JOH, Vol 17, No2, June 1994 pages 127-130 Detection of Early Myocardial Infarction by Gomori Trichrome Aniline Blue Stain (GTAB) Andi At 02:08 PM 7/30/2007, Ford, Judi wrote: >Hi All, > > > >My co-workers and I were wondering what people use for staining >myocardial injuries. We are planning on trying both Masson's and >Gomori's Trichrome, but were told that there is a Gomori's that is >optimized for myocardial injuries. Has anyone heard of this method? >I've done the Gomori's on frozen tissue but we are using formalin fixed >paraffin embedded tissue. > > > >Thanks for any input. > > > >Judi Ford > >Roche Palo Alto > >Palo Alto, CA > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From dhall <@t> incytepathology.com Mon Jul 30 16:50:27 2007 From: dhall <@t> incytepathology.com (Diane Cadorette-Hall) Date: Mon Jul 30 16:52:28 2007 Subject: [Histonet] Reading FISH with Bio View Message-ID: <706224670091FE47997AEF88EFADE7CA2D8760@EXCHANGE-SRV.PAI.E-PATHOLOGY.COM> Hello histonetters. We use the Vysis Her-2/neu kit and are experiencing problems reading the slides on the Bio View. Does anyone else use this combination of test and automated reading? Thanks so much! Diane Diane Cadorette-Hall Histology Laboratory Supervisor InCyte Pathology 509-892-2744 dhall@incytepathology.com From ander093 <@t> tc.umn.edu Mon Jul 30 16:41:30 2007 From: ander093 <@t> tc.umn.edu (LuAnn Anderson) Date: Mon Jul 30 17:12:39 2007 Subject: [Histonet] breast In-Reply-To: References: Message-ID: Hi Anita, It seems to me that I actually saw several responses to the question about fixing breast tissue. Check the archives-the responses should be there. At 04:32 PM 7/30/2007, anita dudley wrote: >I asked this question a few weeks ago and got 1 >answer, what is everyone doing about the new >cap requirements about fixing breast tissue no >more that 48 hrs if a her2 is to be done? we do >not work on the week end and friday that would >be a problem if we had a breast. thanks for your input. anita >_________________________________________________________________ >See what you?re getting into before you go there. >http://newlivehotmail.com_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Mon Jul 30 17:48:56 2007 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Jul 30 17:50:18 2007 Subject: [Histonet] Cytospin - misshapen cells Message-ID: Sonya, This seems very complex. Why make it too difficult. From reading your post the problem could lie in: 1. Is the PBS isotonic 2 Freezing artefact from the (?inadequate) 10min fixation on ice. Why not cytospin directly from the culture medium? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin S. Sent: Monday, 30 July 2007 11:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cytospin - misshapen cells Hi All, I'm having some trouble with cytospins - the cells/nuclei look very misshapen after spinning. I have used a number of different cell lines and have the same problem with them all. I have tried fixing the cells before spinning with 4% PFA in PBS (for this I centrifge the cells, resuspend in PBS, centrifuge, resuspend in PFA for 10min on ice, centrifuge, resuspend in PBS and cytospin). Unfixed cells were cytopsun in media. The cytospin is set to 500rpm for 5min with low acceleration. After spinning I let them air dry (~5min), wash PBS, fix 4% PFA (if not post-fixed), wash PBS and continue with staining. When staining the nuceli just with Dapi I get some very strange looking nuclei, please see some pics on www.histonet.org the file is called Cytospin Dapi.jpg. Is this normal for cytospins? Any ideas? Thanks Sonya _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From pereirafamily <@t> cox.net Mon Jul 30 16:28:59 2007 From: pereirafamily <@t> cox.net (pereirafamily) Date: Mon Jul 30 17:53:45 2007 Subject: [Histonet] copath plus Message-ID: <000601c7d2f0$a4a26a80$09650744@pereirafarm> Our laboratory is getting a new computer system and would like to see if any other histo labs are using it and whether or not they like it. The histology program is copath plus by cerner. If anyone has any information good or bad could you please email Marilyn Weiss at Marilyn.A.Weiss@kp.org. Thank you all for your time. From Lynne.Bell <@t> hitchcock.org Mon Jul 30 14:30:16 2007 From: Lynne.Bell <@t> hitchcock.org (Bell, Lynne) Date: Mon Jul 30 19:48:13 2007 Subject: [Histonet] HER2 FISH Message-ID: Our lab is considering performing HER2 FISH. Presently, we are not doing any insitu hybridization, so forgive me if I ask some very elementary questions. I have read the procedure for this test and is certainly is "doable". I do have a couple of questions, however. If any of you are performing this test, are you doing it in conjunction with the HER2 immuno stain, or as a stand alone test? If you are doing it instead of the immuno stain, what are you using for controls? That's all I can think of now. Thank you for any and all assistance. Lynne Lynne A. Bell, HT (ASCP) Central Vermont Medical Center P. O. Box 547 130 Fisher Road Barre, VT  05641 802-371-4923 From funderwood <@t> mcohio.org Mon Jul 30 13:48:02 2007 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Mon Jul 30 19:49:35 2007 Subject: [Histonet] not histology related (well, almost) Message-ID: All right Joe! Way to Shake Your Goove Thing and Get It Right Back Where It Started. >>> "Joe Nocito" 7/30/2007 1:11:04 PM >>> Last night I went back in time 30 years (or so). Went to a concert that had Peaches & Herb, Maxine Nightingale, The Village People and KC & The Sunshine Band. I have to tell you, we have been dancing the YMCA song wrong all these years. I was taught by the horses themselves. At the NSH banquet, I'm gonna have to give everyone a lesson (this ought to be enough to get y'all to Denver. I just have to go to Denver. JTT is giving us a dance lesson). That and 2 bucks ought to get you a cup of coffee. Stay tuned. JTT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From garth <@t> apollosci.co.za Tue Jul 31 03:22:57 2007 From: garth <@t> apollosci.co.za (Garth Jerome) Date: Tue Jul 31 04:25:09 2007 Subject: [Histonet] Recycling lab equipment? In-Reply-To: <71437982F5B13A4D9A5B2669BDB89EE40F5040AF@ISS-CL-EX-V1.soton.ac.uk> Message-ID: <000901c7d34c$058647d0$7700a8c0@jhb.apollosci.co.za> Hello Sonya All I can say is that ebay works well. Its amazing what you find there. I have purchased microscope spares, bodies, objectives and a few other bits and pieces at a fraction of the cost. I would suggest giving it a go! Regards Garth Jerome Histologist Apollo Scientific cc Telephone : 27-11-466 7666 Fascimile : 27-11-466 7672 Email : garth@apollosci.co.za -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin S. Sent: 30 July 2007 04:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycling lab equipment? In light of the recent pictures of the under-equipped Russian labs, does anyone know of any organisations (in the UK) which recycle lab equipment? We have a couple of old microscopes and loads of slide-staining racks etc which are gathering dust. The microscopes are very old (1950's possibly!) and not great optically but with a bit of TLC they may be of use to someone. Someone suggested trying to sell them on ebay as antiques!! Sonya _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From louise.renton <@t> gmail.com Tue Jul 31 03:30:49 2007 From: louise.renton <@t> gmail.com (louise renton) Date: Tue Jul 31 05:18:06 2007 Subject: [Histonet] not histology related (well, almost) In-Reply-To: References: Message-ID: Well, THATS an incentive to get my funding organized. Can't wait to see JTT with his Zimmer frame! Louise in South Africa On 7/30/07, Fred Underwood wrote: > > All right Joe! Way to Shake Your Goove Thing and Get It Right Back > Where It Started. > > >>> "Joe Nocito" 7/30/2007 1:11:04 PM >>> > Last night I went back in time 30 years (or so). Went to a concert that > had Peaches & Herb, Maxine Nightingale, The Village People and KC & The > Sunshine Band. > I have to tell you, we have been dancing the YMCA song wrong all > these years. I was taught by the horses themselves. At the NSH banquet, > I'm gonna have to give everyone a lesson (this ought to be enough to get > y'all to Denver. I just have to go to Denver. JTT is giving us a dance > lesson). That and 2 bucks ought to get you a cup of coffee. Stay tuned. > > JTT > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From garth <@t> apollosci.co.za Tue Jul 31 05:32:22 2007 From: garth <@t> apollosci.co.za (Garth Jerome) Date: Tue Jul 31 07:04:36 2007 Subject: [Histonet] not histology related (well, almost) In-Reply-To: Message-ID: <000f01c7d35e$18653160$7700a8c0@jhb.apollosci.co.za> Hello Louise Nice to see a fellow South African Histonetter! Regards Garth Jerome Histologist Apollo Scientific Telephone : 27-11-466 7666 Fascimile : 27-11-466 7672 Email : garth@apollosci.co.za -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise renton Sent: 31 July 2007 10:31 AM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] not histology related (well, almost) Well, THATS an incentive to get my funding organized. Can't wait to see JTT with his Zimmer frame! Louise in South Africa On 7/30/07, Fred Underwood wrote: > > All right Joe! Way to Shake Your Goove Thing and Get It Right Back > Where It Started. > > >>> "Joe Nocito" 7/30/2007 1:11:04 PM >>> > Last night I went back in time 30 years (or so). Went to a concert that > had Peaches & Herb, Maxine Nightingale, The Village People and KC & The > Sunshine Band. > I have to tell you, we have been dancing the YMCA song wrong all > these years. I was taught by the horses themselves. At the NSH banquet, > I'm gonna have to give everyone a lesson (this ought to be enough to get > y'all to Denver. I just have to go to Denver. JTT is giving us a dance > lesson). That and 2 bucks ought to get you a cup of coffee. Stay tuned. > > JTT > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From John.MacDougall <@t> ipi.com Tue Jul 31 09:01:49 2007 From: John.MacDougall <@t> ipi.com (John MacDougall) Date: Tue Jul 31 09:33:15 2007 Subject: [Histonet] Smushed tubing Message-ID: <781E27B31A89A040BC9AEE38D04AF42EA0F990@ipimail02.infinitypharm1.com> Hi All We have a Tissue Tek VIP 3000 tissue processor and are in need of some replacement tubing...the tubing we need runs from a regent reservoir (i.e. xylene, ethanol...) into the back of the machine. One of the reservoirs was replaced improperly and deformed the tubing. I figure we need the inner diameter, outer diameter and the material from which the tubing is made and we should be able to order from Fisher or VWR... Thanks John MacDougall This message and any attachments may contain confidential information and/or privileged material. If you received this message in error, please contact the sender and delete this message and any attachments immediately. Thank you. From gvdobbin <@t> ihis.org Tue Jul 31 08:52:05 2007 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Tue Jul 31 09:39:26 2007 Subject: [Histonet] Attn BondMax users-Can I ask a few questions? Message-ID: Hi Folks, I am currently considering the BondMax automated stainer for IHC in my lab. I would like to ask some current users a few questions about your experience with the instrument to help me with my decision. I can either call you on the telephone or if you prefer, I can provide my questions in an e-mail back to you. I have about 10 questions and it should only take 5 or 10 mins to answer them. If any of you can spare the time it would be most helpful. Thank you. Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. Déclaration de confidentialité Le présent message (y compris les annexes) peut contenir des renseignements confidentiels ayant pour objet une personne ou un organisme particulier. Si vous avez reçu la présente communication par erreur, veuillez en informer l'expéditeur immédiatement. Si vous n'êtes pas le destinataire prévu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer complètement de votre système informatique. From ree3 <@t> leicester.ac.uk Tue Jul 31 09:32:50 2007 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Tue Jul 31 09:47:06 2007 Subject: [Histonet] interesting pictures In-Reply-To: <000001c7cfa0$b4a86400$6412a8c0@dielangs.at> References: <000001c7cfa0$b4a86400$6412a8c0@dielangs.at> Message-ID: One wonders at the motivation of putting such information on the web, was it to elicit sympathy for or to heap derision on the unfortunate workers. There were many contradictions for example a relatively new Leica cryostat, embedding centre and rotary microtome, also a relatively new tissue processor next to ancient incubators and sledges and not a disposable/paper towel in sight. The lab personnel obviously treated the lab as a home from home with domestic kettles and irons present, flowers on the window sill, daily newspaper on the desk, together with plastic drinking water bottles and coca cola, which suggests a certain amount of disposable income above the minimum that is assuming they do not refill the bottles from the tap!!!) Although the facilities are largely basic, the level of even basic laboratory housekeeping is deplorable, which is indicative of poor management or poor morale or both, also labelling of chemical/solutions is either minimal or completely non existent, but the 3 women pictured are at least mostly wearing lab coats and have access to disposable gloves even if they are not wearing them in most of the photos, and in the one case they are they look like the modern neoprene sort, but they are all at least smiling for the camera. The electrics are also a mishmash with some quite modern switches and lights contracting with the spaghetti like mess near some power points, also a relatively modern portable/mobile 'phone is visible on one desk. Among the mess that is the celloidin embedding is a very modern looking metal "hockey stick" perhaps picked up on an away day to the West!!. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: 26 July 2007 17:19 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] interesting pictures Hi all, I found these interesting pictures of a Russian pathology in the web. http://www.freewebs.com/ruspat1/biospylaboratory.htm Gudrun From b-frederick <@t> northwestern.edu Tue Jul 31 08:03:10 2007 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Tue Jul 31 11:24:15 2007 Subject: [Histonet] stains for myocardial injury In-Reply-To: Message-ID: <000e01c7d373$289d84a0$d00f7ca5@lurie.northwestern.edu> Judi, You could also try a Goldner's trichrome. We had a researcher using for dog hearts and it is a pretty stain. The research was for following a nerve pathway. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ford, Judi Sent: Monday, July 30, 2007 4:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] stains for myocardial injury Hi All, My co-workers and I were wondering what people use for staining myocardial injuries. We are planning on trying both Masson's and Gomori's Trichrome, but were told that there is a Gomori's that is optimized for myocardial injuries. Has anyone heard of this method? I've done the Gomori's on frozen tissue but we are using formalin fixed paraffin embedded tissue. Thanks for any input. Judi Ford Roche Palo Alto Palo Alto, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Tue Jul 31 11:35:13 2007 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Tue Jul 31 12:18:38 2007 Subject: [Histonet] stains for myocardial injury In-Reply-To: <000e01c7d373$289d84a0$d00f7ca5@lurie.northwestern.edu> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3203FF9FD7@sjhaexc02.sjha.org> Our research techs use Movat's Pentachrome..do you need details? Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Bernice Frederick Sent: Tuesday, July 31, 2007 9:03 AM To: 'Ford, Judi'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] stains for myocardial injury Judi, You could also try a Goldner's trichrome. We had a researcher using for dog hearts and it is a pretty stain. The research was for following a nerve pathway. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ford, Judi Sent: Monday, July 30, 2007 4:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] stains for myocardial injury Hi All, My co-workers and I were wondering what people use for staining myocardial injuries. We are planning on trying both Masson's and Gomori's Trichrome, but were told that there is a Gomori's that is optimized for myocardial injuries. Has anyone heard of this method? I've done the Gomori's on frozen tissue but we are using formalin fixed paraffin embedded tissue. Thanks for any input. Judi Ford Roche Palo Alto Palo Alto, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From kmilne <@t> bccancer.bc.ca Tue Jul 31 13:27:47 2007 From: kmilne <@t> bccancer.bc.ca (Milne, Katy) Date: Tue Jul 31 13:28:50 2007 Subject: [Histonet] McLetchie's Trichrome Message-ID: <07979E76B0869D4E8C9FE4AA9FC06578046689A3@srvex03.phsabc.ehcnet.ca> Hi everyone, I'm wondering if anyone has ever used the McLetchie's trichrome stain (no Bouin's!)? How did it compare to Masson's/Gomori's? I'd like to show the stroma (preferrably reactive stroma) in mouse FFPE tumours. Thanks, Katy Milne Deeley Research Centre BC Cancer Agency From gcallis <@t> montana.edu Tue Jul 31 10:15:25 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Jul 31 14:02:23 2007 Subject: Hemtoxylin basic fuchsin picric acid stain for myocardial infarction Re: [Histonet] stains for myocardial injury In-Reply-To: <6.2.3.4.1.20070730144421.01f42d00@algranth.inbox.email.ari zona.edu> References: <6.2.3.4.1.20070730144421.01f42d00@algranth.inbox.email.arizona.edu> Message-ID: <6.0.0.22.1.20070731090607.01b14c10@gemini.msu.montana.edu> I have done the Hematoxylin Basic fuchsin Picric acid stain for human myocardial infarction, but do not have the protocol on computer (old hard copy only left over from the "BC" aka before computers). It was published in Laboratory Medicine back in the 70's but I do not have the specific reference for it. I will be happy to copy and send or retype it on computer, it is not difficult to do. There is an alum hematoxylin used with mercuric oxide in the recipe for staining nuclei. I recommend trying a commericial hematoxylin or use a Weigerts iron hematoxylin as substitutes to avoid the toxic chemical omponent. An acutely ischemic myocardium will be crimson red with light brown normal mycardium surrounding it. Question for Andi: Does the JOH publication list this as a reference? Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 At 03:47 PM 7/30/2007, you wrote: >This is the stain I have done for one of the investigators here: > >JOH, Vol 17, No2, June 1994 pages 127-130 >Detection of Early Myocardial Infarction by Gomori Trichrome Aniline Blue >Stain (GTAB) > >Andi > > > > >At 02:08 PM 7/30/2007, Ford, Judi wrote: >>Hi All, >> >> >> >>My co-workers and I were wondering what people use for staining >>myocardial injuries. We are planning on trying both Masson's and >>Gomori's Trichrome, but were told that there is a Gomori's that is >>optimized for myocardial injuries. Has anyone heard of this method? >>I've done the Gomori's on frozen tissue but we are using formalin fixed >>paraffin embedded tissue. >> >> >> >>Thanks for any input. >> >> >> >>Judi Ford >> >>Roche Palo Alto >> >>Palo Alto, CA >> >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >..................................................................... >: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : >: Sr. Research Specialist University of Arizona : >: (office: AHSC 4212) P.O. Box 245044 : >: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : >: (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : >:...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Tue Jul 31 14:28:55 2007 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Jul 31 14:30:01 2007 Subject: more on RE: [Histonet] stains for myocardial injury In-Reply-To: <6.0.0.22.1.20070731090607.01b14c10@gemini.msu.montana.edu> References: <6.2.3.4.1.20070730144421.01f42d00@algranth.inbox.email.arizona.edu> <6.0.0.22.1.20070731090607.01b14c10@gemini.msu.montana.edu> Message-ID: <6.0.0.22.1.20070731132454.01b005b0@gemini.msu.montana.edu> After some digging into the protocol, Hrapchak and Sheehan's 2nd edition of Theory and Practice of Histotechnology, p 199 (with original #39 cited publication from 1971 by Lie et al) has the hematoxylin, basic fuchsin, picric acid stain for early myocardial ischemia. It is easy to do and has worked well for us on human tissue. It can be used for animal tissue. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 From algranth <@t> u.arizona.edu Tue Jul 31 10:58:39 2007 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Tue Jul 31 14:35:28 2007 Subject: Hemtoxylin basic fuchsin picric acid stain for myocardial infarction Re: [Histonet] stains for myocardial injury In-Reply-To: <6.0.0.22.1.20070731090607.01b14c10@gemini.msu.montana.edu> References: <6.2.3.4.1.20070730144421.01f42d00@algranth.inbox.email.arizona.edu> <6.0.0.22.1.20070731090607.01b14c10@gemini.msu.montana.edu> Message-ID: <6.2.3.4.1.20070731084724.01ed4b38@algranth.inbox.email.arizona.edu> Gayle, The article lists a reference from Poley RW, Forbes CD, hall MJ: Fushsinophilia in early myocardial infarction, a method for the demonstration of early myocardial infarction using acid fuchsin staining. Arch Path 77:325-329, 1964 also Lie JT: Detection of early myocardial infarction by the acid fuchsin staining technic. Am J Ckin Path 50:317-319, 1968 I would like to see a copy of your Lab Medicine protocol since I'm currently involved in a project trying to demonstrate early MI using mouse hearts - I was doing Mallory trichrome at the request of the PI and getting strange results. In fact he was just here looking at the results with me. Jury is still out on the stain. Thanks. Andi At 08:15 AM 7/31/2007, Gayle Callis wrote: >I have done the Hematoxylin Basic fuchsin Picric acid stain for >human myocardial infarction, but do not have the protocol on >computer (old hard copy only left over from the "BC" aka before >computers). It was published in Laboratory Medicine back in the >70's but I do not have the specific reference for it. > >I will be happy to copy and send or retype it on computer, it is not >difficult to do. There is an alum hematoxylin used with mercuric >oxide in the recipe for staining nuclei. I recommend trying a >commericial hematoxylin or use a Weigerts iron hematoxylin as >substitutes to avoid the toxic chemical omponent. > >An acutely ischemic myocardium will be crimson red with light brown >normal mycardium surrounding it. > >Question for Andi: Does the JOH publication list this as a reference? > >Gayle Callis HTL, HT, MT(ASCP) >Research Histopathology Supervisor >Veterinary Molecular Biology >Montana State University >Bozeman MT 59717 > > >At 03:47 PM 7/30/2007, you wrote: >>This is the stain I have done for one of the investigators here: >> >>JOH, Vol 17, No2, June 1994 pages 127-130 >>Detection of Early Myocardial Infarction by Gomori Trichrome >>Aniline Blue Stain (GTAB) >> >>Andi >> >> >> >> >>At 02:08 PM 7/30/2007, Ford, Judi wrote: >>>Hi All, >>> >>> >>> >>>My co-workers and I were wondering what people use for staining >>>myocardial injuries. We are planning on trying both Masson's and >>>Gomori's Trichrome, but were told that there is a Gomori's that is >>>optimized for myocardial injuries. Has anyone heard of this method? >>>I've done the Gomori's on frozen tissue but we are using formalin fixed >>>paraffin embedded tissue. >>> >>> >>> >>>Thanks for any input. >>> >>> >>> >>>Judi Ford >>> >>>Roche Palo Alto >>> >>>Palo Alto, CA >>> >>> >>> >>>_______________________________________________ >>>Histonet mailing list >>>Histonet@lists.utsouthwestern.edu >>>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >>..................................................................... >>: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : >>: Sr. Research Specialist University of Arizona : >>: (office: AHSC 4212) P.O. Box 245044 : >>: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : >>: (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : >>:...................................................................: >> http://www.cba.arizona.edu/histology-lab.html >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From kmerriam2003 <@t> yahoo.com Tue Jul 31 11:27:25 2007 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Tue Jul 31 15:27:37 2007 Subject: [Histonet] Histology duties in necropsy Message-ID: <82465.91188.qm@web50312.mail.re2.yahoo.com> Hi Carol, I have worked in several different companies (biotech/pharma/CRO) and where the line is drawn between necropsy and histology can vary greatly. This is what I have seen: at a CRO that I worked at (we did primarily GLP tox-path) the pathology department included technicians that did the necropsies and organ weights (and everything that came after that), but the in-vivo group did all of the blood collections at a biotech company I worked at, the in vivo technicians did all of the necropsies and gave us tissues in formalin (sometimes they would trim it, sometimes the histologists would need to trim it; it depended on the study and the type of tissue) at my current job (early-discovery research at a biotech) and my previous job (research (not tox) at a big pharma), there is a combination of both. Sometimes the pathology department does the necropsies, sometimes not; it depends on the skill level of the researcher, their availablity (and our availability) and the type of study. If the researchers are uncomfortable doing them, we do the necropsies for them. So the take-home message based on my experience at 4 different companies is that it just depends on the situation. Kim Merriam, MA, HT(ASCP) Cambridge, MA ----- Original Message ---- From: "Wilson, Carol" To: histonet@lists.utsouthwestern.edu Sent: Monday, July 30, 2007 3:28:31 PM Subject: [Histonet] Histology duties in necropsy Hi All, Taking an informal survey of anyone involved in vet histology, primarily research. How many histotechs out there actually perform necropsy or have histology departments that oversee that area? Thanks, Carol Carol Wilson Team Leader - Associate Scientist III - Histopathology Ricerca Biosciences, LLC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Boardwalk for $500? In 2007? Ha! Play Monopoly Here and Now (it's updated for today's economy) at Yahoo! Games. http://get.games.yahoo.com/proddesc?gamekey=monopolyherenow From contact <@t> excaliburpathology.com Tue Jul 31 11:53:41 2007 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Tue Jul 31 15:53:51 2007 Subject: [Histonet] Smushed tubing Message-ID: <64563.58376.qm@web50112.mail.re2.yahoo.com> Hi John, I recommend taking a piece to a local tubing and hose company. They will only charge you cents per foot instead of mega bucks plus shipping from a medical supply. Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 630 N. Broadway Moore, OK 73160 405-759-3953 contact@excaliburpathology.com www.excaliburpathology.com From judi.ford <@t> roche.com Tue Jul 31 16:53:18 2007 From: judi.ford <@t> roche.com (Ford, Judi) Date: Tue Jul 31 16:53:26 2007 Subject: [Histonet] Enzyme staining Question Message-ID: Hi Everyone, First I want to thank you all for the information on the different stains for the myocardial ischemia. We have a plan to work with now and alternatives if the pathologist asks for more work. My next question involves enzyme staining. I am doing a Naphthol AS-D Chloroacetate Esterase procedure that requires aqueous mounting media. This may be a dumb question but I was wondering if its better to do the procedure and examine right away or can the finished slide be examined at a later (day or two) without any fading artifact happening? Judi Ford Roche Palo Alto From AnthonyH <@t> chw.edu.au Tue Jul 31 16:55:32 2007 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Jul 31 16:55:54 2007 Subject: [Histonet] Attn BondMax users-Can I ask a few questions? Message-ID: We have had the Bond Max for two years now and it works a treat Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Greg Dobbin Sent: Tuesday, 31 July 2007 11:52 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Attn BondMax users-Can I ask a few questions? Hi Folks, I am currently considering the BondMax automated stainer for IHC in my lab. I would like to ask some current users a few questions about your experience with the instrument to help me with my decision. I can either call you on the telephone or if you prefer, I can provide my questions in an e-mail back to you. I have about 10 questions and it should only take 5 or 10 mins to answer them. If any of you can spare the time it would be most helpful. Thank you. Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ayant pour objet une personne ou un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer compl?tement de votre syst?me informatique. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Tue Jul 31 17:00:37 2007 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Jul 31 17:00:56 2007 Subject: [Histonet] Enzyme staining Question Message-ID: We use the fuchsin method and the fading is not apparent (ie fades as much as other techniques using fuschin). There is no need for an aqueous mountant, just dehydrate, clear and mount with a DPX type of mountant. Method as follows: Non-Specific Esterase Principle Esterase is capable of hydrolysing carboxylic acid esters. The technique is an azo-coupling method where the enzyme releases alpha-napthyl from alpha-napthol acetate, which then couples to para-rosanilin to produce and insoluble azo dye. Fixation notes 1. If tissue fixed in mercury containing fixative, do not remove any mercury pigment with iodine. 2. For smears, fix for 2 minutes in absolute methanol containing 10% formaldehyde by volume and then wash. 3. Do not overheat slides when drying. 4. Do not fix tissue in an acid fixative, e.g. Zenker, Bowins. 5. Do not decalcify tissue in acid solutions use EDTA. Microtomy 5?m paraffin sections Solutions 1. Pararosaniline-HCl stock solution Warning: Suspected Carcinogen - see MSDS 2. 4% sodium nitrite. Warning: Toxic - see MSDS Sodium Nitrite 0.8 g Distilled water 20 ml 3. Sorensen 0.1M Phosphate Buffer, pH 6.5 Sodium dihydrogen orthophosphate (MW 156) 2.648g Disodium hydrogen orthophosphate (MW142) 1.135g Distilled water 500ml 4. Napthol AS-D Chloroacetate. (Sigma N-0758 1g) 5. N,N - Dimethylformamide. Warning: Suspected Carcinogen - see MSDS 6. Harris Haematoxylin Incubation solution 1. Mix 0.2ml of pararosaniline solution (A), with 0.2ml of 4% sodium nitrate (B), mix and let stand for 1 minute. 2. Add 35ml of phosphate buffer (C). 3. Dissolve 0.01gm Napthol Chloroacetate (D), in 1 ml Dimethyl formanide (E). 4. Mix solution prepared in step 2 with 3. Controls Both small intestine and kidney serve as good control material for non-specific esterases that are found in the lysosomes and endoplasmic reticulum of cells. Method 1. Deparaffinise and hydrate sections. 2. Place slides into incubation solution and incubate for 30 minutes. 3. Check microscopically if reaction is not complete, refilter and replace slides for 15-30 minutes more. 4. Wash slides in water for 5 minutes. 5. Counterstain in Harris's haematoxylin 2 min. 6. Wash in tap water 3 min. 7. Dehydrate, clear and mount. Reference 1. Leder, L.D., Klinische Wochenschrift, 1964, 42 (ii); 553. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ford, Judi Sent: Wednesday, 1 August 2007 7:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Enzyme staining Question Hi Everyone, First I want to thank you all for the information on the different stains for the myocardial ischemia. We have a plan to work with now and alternatives if the pathologist asks for more work. My next question involves enzyme staining. I am doing a Naphthol AS-D Chloroacetate Esterase procedure that requires aqueous mounting media. This may be a dumb question but I was wondering if its better to do the procedure and examine right away or can the finished slide be examined at a later (day or two) without any fading artifact happening? Judi Ford Roche Palo Alto _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From Maxim_71 <@t> mail.ru Mon Jul 30 14:01:50 2007 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Wed Aug 1 02:37:32 2007 Subject: [Histonet] interesting pictures Message-ID: <1341615539.20070730230150@mail.ru> Dear histonetters! Pictures, which you saw are really. So or approximately so look the majority histo labs in Russia. Our country has also very progressive and rich labs. I belive that now we today have much possibilities for improvement of the work conditions in our labs. You help to us by your advices. It seems that we shall reach the modern level much quicker, than you came hereto its way. The process of the development does not look at condition purse or wit. If something must be developed, it will is developed earlier or later. It is possible that we can turn out to be in side, if we can not participate in this themselves. Thank you all. Maxim Peshkov Russia, Taganrog.